LOCUS       DTVD01000001            3538 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10060_length_3538_cov_390.853287, whole genome
            shotgun sequence.
ACCESSION   DTVD01000001 DTVD01000000
VERSION     DTVD01000001.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_3538_cov_390.853287"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="EYO68_00005"
     CDS             <1..687
                     /locus_tag="EYO68_00005"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIC06221.1"
                     /translation="QESIKAVSEDPELELVAQTDLGDNLSESISQSEAQVVVDFTIAS
                     SALKNTVCIIESGARPVIGTSGLLPEEIAELQALCTKHKLGGIIAPNFAIGAVLMMKY
                     SQDAAKYFPHAEVIELHHDGKVDAPSGTAIKTANLLAESRSSVPKKIADKEIISGARG
                     ANAENIRVHSVRLPGLVAHQEVIFGGQSQTLTIRHDSIHRDSFMPGVCLACKKVMDLN
                     ELVYGLEHLL"
     gene            complement(701..1630)
                     /locus_tag="EYO68_00010"
     CDS             complement(701..1630)
                     /locus_tag="EYO68_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase XerD"
                     /protein_id="HIC06222.1"
                     /translation="MQPLVSEFLDTLQGKKYAPNSIQSHRLDLHRFLRWLGIDEEESD
                     SQKLLVKLRKLNRQDIESYLSFLSESYKPRTLARHISSLKLFLDHLELSGLIKTSPAH
                     QIPFPAVLPEAPEILSPEEVVALLEVPSLEHYLGLRDRAMLELLYSSGLKVKELLGLD
                     VEDLFLDLEFLKVRSKRERMVPMTSKAVEVLRTYLEKAREQRLLNPEDKCLFPSRNGI
                     RMSRVGFWAMIKKHALRAGITSRINPRILRHSFAVHLLQNGIDLSDIKDLFGYVSLDA
                     TLQYAHVNRPDFFEVYHELHPRGRKHTEQSSIT"
     gene            complement(1705..1887)
                     /gene="ssrS"
                     /locus_tag="EYO68_00015"
     ncRNA           complement(1705..1887)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="EYO68_00015"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2030..2335)
                     /locus_tag="EYO68_00020"
     CDS             complement(2030..2335)
                     /locus_tag="EYO68_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIC06223.1"
                     /translation="MQENDSKQYQINISGNRFSISSPYGEEHVREVEKFLGQQIQEVS
                     EQTEEFGPSSIALLVALNLADKLLTLQKKVTKFDGIEADLQTLYYRLDKVLAEKKTD"
     gene            2561..3454
                     /locus_tag="EYO68_00025"
     CDS             2561..3454
                     /locus_tag="EYO68_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06224.1"
                     /translation="MKSSPLSQLSMESQQEFGALLLLDQLMRYDLLEVEKDNLTETVS
                     LLEKEVAELKKGFFHSDEQDQELSFEKDELREAKEALSQVEKEMKENDHCRLNLALAE
                     TDDEGLEPLLKFMEERGTLTVSDDNFYQPTKKGREVYQHLVEQLEAYVVHFGIYTYVD
                     LDEGAFGEPKTDLLEGDQWSDLRVAVAEHKGIDQYRVVFLAMLSAERFFENPDWKFDL
                     SMGTLFDEMQQIVQDQLCVEDLGYTDNDGQVSGEDVIRDIIEQGEKLSRERRQQEQET
                     EEKEQAEAEPDEQVIRATYYW"
BASE COUNT         1040 a          755 c          784 g          959 t
ORIGIN      
        1 caggaatcca ttaaagctgt cagtgaagat cctgaattgg aactagttgc tcaaacggat
       61 ttaggtgata atctttctga atcaatcagt caatccgagg cccaggttgt ggtggacttc
      121 actattgcct cttctgcatt gaaaaacact gtctgcatca tcgaatctgg agcccggcca
      181 gtaattggta ccagcggact gcttccggaa gagattgcag aactgcaggc gctttgcaca
      241 aaacataaac ttggcggcat catagctccg aattttgcca ttggcgcagt gctgatgatg
      301 aaatattctc aggatgcagc caaatatttt ccgcacgctg aagtgataga actgcatcat
      361 gacggtaaag tggatgcacc ttccggaact gcaatcaaaa cagcaaattt acttgctgaa
      421 tcacgaagtt cagttccaaa aaagattgct gataaagaaa ttatctccgg agcaagaggg
      481 gccaatgccg aaaatatccg tgttcactca gtccggcttc ccggtttggt tgcacaccag
      541 gaagtgattt ttggcggcca aagccaaacc ttgaccatac gtcacgattc tatccaccgt
      601 gactctttta tgccgggagt ctgcctggcc tgcaaaaaag tgatggattt gaacgaactc
      661 gtttatggac tggagcatct gctttaagtg aaaaaagaaa ctatgttatg gaagattgct
      721 ctgtatgttt tcgtccccgc ggatgcagtt catgatatac ctcgaaaaaa tcgggccggt
      781 ttacatgtgc gtactgcagg gttgcatcaa gactgacgta gccgaacaaa tccttaatat
      841 ctgacaggtc aataccgttt tgcagtagat gtactgcaaa tgaatgacgc agaatccttg
      901 gattgatgcg actggtgatg cctgcacgaa gagcgtgttt tttgatcatt gcccaaaatc
      961 caaccctgct catgcgtatt ccgtttcgac tcggaaataa acatttatct tccggattca
     1021 gcagacgttg ttctcttgcc ttttccagat atgtccgcag tacctccaca gcttttgagg
     1081 tcatcggtac catgcgctcg cgttttgaac gaaccttcaa aaactccaaa tccaaaaaca
     1141 gatcctccac atccagtcca agcagttctt tcactttcaa accgcttgaa tacagaagtt
     1201 ccaacattgc cctgtcccgc aaccccagat aatgctcaag tgaaggcact tcgagcagtg
     1261 cgacaacttc ttccggtgac agtatttcag gagcctccgg caaaactgca ggaaaaggta
     1321 tctgatgtgc cggactggtt tttatcaatc cgctaagttc caggtgatcg agaaaaagtt
     1381 tcagggacga aatgtgtcga gccaaggttc ttggcttata ggattcacta agaaaagaga
     1441 ggtagctttc aatatcctgc cgatttagtt tgcggagctt cacgagaagt ttttggctgt
     1501 cgctttcctc ctcatctatt cccagccacc gcaaaaatct gtgcaggtcc agacggtggc
     1561 tctggatact gtttggagca tattttttgc cctgcagtgt gtctagaaac tccgaaacca
     1621 gcggctgcat atttttttta actggtccgg tggaaagtga aacggctgca ttctaaaata
     1681 tgccgtatga aaaaatgaaa tgaaaagaag cccgcgatgc cgtgtgtaaa atggcaattg
     1741 ttacctcgtt aaacaaggtg gagtaatgtg gtaaaaggtt cgggcttcct gctctacggc
     1801 tggcctgcac acccccttcc agggacgaac tccttttttt taatatcagg tagcaaaagt
     1861 tttacatcac gaacaccgca gactccaatg ctctgtacta agcttactat ctctgcagta
     1921 aaaatcaata acgttaaaaa actaaattat attctgcctc ataaaagatt aacgacatta
     1981 aactcaagac tcccgcttga gattaacagc caattattgt cagggttcct taatcagttt
     2041 tcttttcagc taatactttg tccagtcgat aatataatgt ttgcagatca gcttcaatgc
     2101 cgtcaaactt ggttactttt ttttgcagcg tcagcagttt atcagccaaa ttcaaggcaa
     2161 ccagcaaagc tatgcttgaa ggaccaaatt cttctgtttg ttcagaaact tcctgaatct
     2221 gctggcccag aaacttctcc acctcacgga cgtgttcttc accatacgga ctggaaattg
     2281 agaaacgatt accgctgata ttgatctggt actgcttact gtcgttctcc tgcacaatta
     2341 cctcttttcg actaactgtt taataatccg cattggagca gatcttaaat tatttattcg
     2401 tgacgcacat ccctggaacc tgatgctaat aattgctgga tgtgatacag tagacaccat
     2461 tctaactcat aacttagttt ctaacaggtt ttttttaaga aagcaatatc atattagaat
     2521 aaatgacaaa attgggatca atttgtatta agaataaatt atgaaatctt ctcctttaag
     2581 tcaattgagc atggaatcgc agcaagaatt tggcgcactg ctcctgctgg atcagctgat
     2641 gcgctacgat ctgcttgaag ttgaaaaaga caatttgact gaaacagtca gtctgctgga
     2701 aaaagaagtt gcagaactga aaaaaggttt ctttcactct gatgaacagg atcaggagct
     2761 aagctttgaa aaagatgaac tcagagaagc aaaagaggca ctttctcaag ttgaaaaaga
     2821 aatgaaggaa aacgatcatt gtcgcctgaa tcttgccctt gctgaaactg acgatgaggg
     2881 cctcgagccc ctgttgaaat ttatggagga acgaggcacc ttgactgtca gtgacgataa
     2941 tttttatcag ccgaccaaaa aaggccggga agtatatcag catttggttg agcagcttga
     3001 agcttatgtt gtccattttg gcatctatac ttatgtggat ctggatgagg gtgcattcgg
     3061 ggaacctaaa acggatttgc tggaaggcga tcaatggtcc gatttgcgtg ttgctgttgc
     3121 agaacacaaa ggcatagacc aataccgggt tgtattttta gccatgctga gtgcagaaag
     3181 attttttgaa aacccggact ggaaattcga tttgagcatg ggtacgcttt ttgacgaaat
     3241 gcagcaaatt gtacaggatc aactctgtgt ggaagatttg ggttacactg acaatgacgg
     3301 tcaagtttcc ggagaagatg ttatccgtga cataattgaa caaggcgaga aactatcacg
     3361 tgaacgacgc cagcaggaac aggaaaccga agaaaaagag caagctgagg cagagccgga
     3421 tgagcaggtc ataagggcaa cctactactg gtaaaaaaat atgtacttaa aaaacagaga
     3481 aatagaaggt ccggatcaac ttgggaaagg ttgggacact ccagacataa actggtat
//
LOCUS       DTVD01000002           13323 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1010_length_13323_cov_395.495252, whole genome
            shotgun sequence.
ACCESSION   DTVD01000002 DTVD01000000
VERSION     DTVD01000002.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_13323_cov_395.495252"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(100..999)
                     /gene="rarD"
                     /locus_tag="EYO68_00030"
     CDS             complement(100..999)
                     /gene="rarD"
                     /locus_tag="EYO68_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIC06225.1"
                     /translation="MSNFIKSQHTEAQIGIGYALLAFSAWGFLPIYWKLLDTVPSLEI
                     LAHRMVWSVLFLVGLLAVQKRLGEFRDLLKTPKYIWMLLGTAVLLGVNWFVYIYGVNT
                     NQIVETSLGYFINPLFNVLLGAIFLKERLNYWQSLALGMAALGVLNFLWDFDSLPWIA
                     LSLAFTFSFYGLIRKMIPVKPLVGLLMETLLLAPFAAVMIVVWNVDGTGNIGGDWRIV
                     IFLVGAGVVTSLPLLWFTNAGKRLRYTTLGFIQYMTPSIQLLIGVYLYHEPFTPTHSI
                     TFGLIWAGLVIFSINSLFVQSPS"
     gene            complement(1015..2589)
                     /locus_tag="EYO68_00035"
     CDS             complement(1015..2589)
                     /locus_tag="EYO68_00035"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIC06226.1"
                     /translation="MFQSLRHKIILVAAVCASITLLGFVALYWLTLEQHQSLDRHRDL
                     PAVSLKWFSLNNAVHKASLAQKAWIDSGDLKFIKERDLTWAVDIHPAFKQLDLLYKKV
                     RVWEGERSVERRVFYDLRLMILSLENLQKNTSDPEQKLTLEEAQIIWTDKESPLVQNI
                     SSQVEIMIRWQTDFARQQESELRQGLSGLGIWIWVVASVVLLLVLGLAVYFANRITAP
                     LRKLRGAAQQLIRDQFHKPAEKSIEVEGKADEFPENMDEVQTLTEVFQKMESVIRERT
                     ELLETSNRQLDQASRAKGMYLTNMSHELRTPLNAIIGFAEVLLESGREEQLSDYQIDR
                     LSRILTSGQHLLELINSLLDLSKIEAGQMQIFNSEFQLENLLQDVMEWLEPLMQKKSL
                     QHKLVFLSDSPILLHSDSGKVRQVLINLLGNAIKFTESGGHIKVRSFRDATGVSVEIQ
                     DTGCGITVDQQQQIFEVFHQVNSSGTLKGTGLGLALVQSLMKLLGGSVSLKSKYGKGS
                     TFTLHFPASSRLKHES"
     gene            3054..3803
                     /locus_tag="EYO68_00040"
     CDS             3054..3803
                     /locus_tag="EYO68_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HIC06227.1"
                     /translation="MVRDAGLYALQWFRKLDSLKTEKKGPQDLVSKADREVEKMIRER
                     LQTLFPEDGFLGEESGTRNLDAEGIWIVDPIDGTICFLNGLSSWCVSIAFVVKNQIEI
                     GLIYAPCTDELFASQRGTGATLNGKPMKPSKATTLADGIVGLGYSPKSSIVATQKAFD
                     YLFKNGGVYHDVGSAALMLAYVAAGRYIGVYEFQINSWDCLAGIAMVRETGGWTNDFL
                     ANDGLLTGNPVLVAAPGVKDAMQNLFAAAGH"
     gene            3830..5914
                     /locus_tag="EYO68_00045"
     CDS             3830..5914
                     /locus_tag="EYO68_00045"
                     /inference="COORDINATES: protein motif:HMM:PF00128.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HIC06228.1"
                     /translation="MFQSIRPLPNPSMGGHWRGNSFQFGLIAEHVVSIRYLFYPHFFP
                     QLEGNQALLSDVQLDTSYQFGDVWGWEAEFSGSAEEHMVYLIQLQEKDGNTKFVFDPF
                     ARESFGGEHWGRPIGFVVDEEDFRLSVISRPKSKVFHGMRRLPVLRQETPTDELTASR
                     PHRPHHPLEQSVIYECHVRGMTNSPSISVSGSSQSGTYRGLVECIPYLKALGVTAIEL
                     LPVFDFDENERIITDAEIEHTVLNYWGYSPLLFFAPKQNYASDVVNPVREFKAMVDKF
                     HEADLEIWLDVVFNHTAEFGDNGYADHFKLLAPDQWYLREKDGSYKNYSGCGNTLNCA
                     HPTTRRMIRDCLIYWSHVMGVDGFRFDLASILNRDSAGDIMDFPQLLWELRHEPSLKN
                     IKLISEPWDAAGAYELGKAAEFADWTEWNDQFRDKVRRAVRGDEGMMEGLKESILGSP
                     NIFGSVEKGRKRSLNFVTSHDGMTLMDLVSYSEKHNEANGEENRDGHSADFSDNCGTE
                     GVTDDPEILSLRFRKLRMFHCLLQLSNGIPMLLGGDEFGRTQQGNNNAYCHDSALTWL
                     DWNLTEQNSVLLAFTRRMIAFRKQHAPFLFSEKSKYKWFNASGGPEEFAPYVRTLHFE
                     VTNPGWPDQAMRILINCFDKPVKFNLPEEIAWNVLIDTEKEPAEFVSPVGGIAWLEGF
                     SVQVLRGHSVDG"
     gene            6139..6546
                     /locus_tag="EYO68_00050"
     CDS             6139..6546
                     /locus_tag="EYO68_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIC06229.1"
                     /translation="MQGGCLCGAVKFKLTGELRPVINCHCGQCLHTHGNFAAYTSVEK
                     KNFQLVNDVGLKWFRSSNEARRGFCQECGASIFFERLGGSNISIAAGMLDSSKGLKTV
                     EHIFVDDKPDYYEIEDDLPKFSQYYKRQLESDT"
     gene            complement(6792..7844)
                     /gene="queA"
                     /locus_tag="EYO68_00055"
     CDS             complement(6792..7844)
                     /gene="queA"
                     /locus_tag="EYO68_00055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIC06230.1"
                     /translation="MPSHDWKLSSYDYKLPQSLIAQHPAPKRDQSRMLFLERKSGKIS
                     NRNFTEIVSLLPESSCLVINNTKVLPARIPGKRQSGGKIEALLIEENNNGDWSAIVRK
                     AGRIKQGERLEFCDGKLLAKAKERLEEGEWLLEFEETERLKERLEDVGLPPLPPYIER
                     RNASDLQNTKDRERYQTCFATEPGAIAAPTAGLHFTPEILTEIGNRGIDILEVTLHVG
                     LGTFSSIDQEDIRKHKMHAEFFSVSSQTLRQLQVYQNNNKQMINVGTTSVRVLETLVR
                     QNFQESSGWTDIFIYPPCKFTMTDGLLTNFHLPKSTLMLLVSAFCGREFLLSSYQHAV
                     AEKYRFFSYGDCMLIL"
     gene            complement(7927..8529)
                     /gene="fliN"
                     /locus_tag="EYO68_00060"
     CDS             complement(7927..8529)
                     /gene="fliN"
                     /locus_tag="EYO68_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIC06231.1"
                     /translation="MLGTGVTPFLPINLLNGVKMVDEEVQEEQENAEMKAAETENGDS
                     PDDDMDSLMNELEQQKTSPEGTGGSGDLSSLITEKADSTEENIDEMLDSASDTTSSMD
                     DTPVEEGVDLEFLQKMPLTMTLEVGRAKMTINDLLSLGQGSVLELHRLVGESLDLFAN
                     GKLIAQGEVIIVNEKFGAKLTNIISPEERIKQMDGMDKTS"
     gene            complement(8493..9437)
                     /locus_tag="EYO68_00065"
     CDS             complement(8493..9437)
                     /locus_tag="EYO68_00065"
                     /inference="COORDINATES: protein motif:HMM:PF06999.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06232.1"
                     /translation="MSDSKFCSFLCRHSGEPLAGTAPFAEHFVFISWPKKYWEYEALE
                     SKGGFPTGLKQWMKEKSKISGKISIRLASQAKLENDKVELFIYPGKWYYSNVLPKEIP
                     EVLESHFQHYKTSDFSCKKIEREQIFICTHGRHDKCCAKFGQKLADQMQYHVQKQNSS
                     VEVWESSHLGGHRFAPTMIDFPSGRVYGRMTPDEIPRFLESRKQGQVYGSAYRGSIFL
                     PELEQVAEAHVQHYCSVQQLDCQVKIKNLEKLREDKFRCTAAFEINKRSAVSQNNIPD
                     ELAFSFKLKDFESPSGCDALEEPKLRKCWELELPLSFQ"
     gene            complement(9471..10514)
                     /locus_tag="EYO68_00070"
     CDS             complement(9471..10514)
                     /locus_tag="EYO68_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC06233.1"
                     /translation="MKQEKNANTLLQIRELVREYILPRQHIFIPPKRFRALDGVSLSL
                     NYGESFGIVGESGCGKSTLARTVLALESPQSGEVCFQGKVLHTLKTKDLRRMRRGMQM
                     VFQDPYGSLDPRHSVSQIVSEPLTLLREVTLKIQQHQVAEALENVGLSPADANKFPHE
                     FSGGQRQRIAIARALITRPSLIVADEPVSALDVSVQAQVLNLMMDLREQHGLAYLFIS
                     HDLSIVRHMTTNVAVMYAGRIVETGPTETLFDHAQHPYTRALLEAVPRPDPSRKRTGR
                     LKKSASTQLFPETEGGCAYASRCPLVEEKCREKSPSLLNLNNSSAMESDKNPAEIVHK
                     VACYKPLDSVVSV"
     gene            complement(10511..11371)
                     /locus_tag="EYO68_00075"
     CDS             complement(10511..11371)
                     /locus_tag="EYO68_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06234.1"
                     /translation="MAAFAIMGLLPENMTASGEVMFGKNLLNLPEKELCKIRGNRISM
                     VFQEPMTALNPVQPIGFQVAESLILHKNLSRHQAQRQASKVLDRVGLPAKKFSRSLYP
                     HQLSGGQRQRVVIAIAMVCEPELIIADEPTTALDVTIQEQILALISELVVEENMALML
                     ITHDLGVVAENTDQMLVMYAGRIVEKGPTNKVFEHLAHPYTRGLFASIPDTEKSANDL
                     SLHPENPKNSRLSTIPGRVPEFWERTKGCSFADRCSRAQAKCFSELPDETRISNGHSA
                     ACFFPWEQNA"
     gene            11792..12670
                     /locus_tag="EYO68_00080"
     CDS             11792..12670
                     /locus_tag="EYO68_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06235.1"
                     /translation="MRSAIQEELFGPEQYTRLCSMCRGEFPRSPEFFPPGRCQDRLAS
                     FCRKCANVRAQVRQATKETERRLSQMNLLEKSCEGCGTIKSLREFYMSSHSHDGKTST
                     CKNCIDAKSSERKMRQQRLGDLAWAVYFIQDSRNNRVKIGSCDDPYVALETLQKGSSE
                     TLHLLASHEAGQKESAENIKSTLHSLFQTHYTGHGWFEMVPSLEQYISLIQQEDYDKA
                     EILLHPMENTQNEAAQKTVPKDITEGAVFVEGQKFPSIAAASNATGYSAEQLKKYIQN
                     SEESGISVTSKLKKAV"
     gene            12695..>13323
                     /locus_tag="EYO68_00085"
     CDS             12695..>13323
                     /locus_tag="EYO68_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC06236.1"
                     /translation="MSGSFYSKFVFPYLCELTMSGKSLSGFRQDALQEINGEVLEIGF
                     GTGLNLPHFPETVQKIDAVDVNPRMHRLARKRIDKSGIAVTNYVLSGEHLPMDDASYD
                     YVVCTFTLCSIPDVSQALLEINRVLKPRGNFVFLEHGLSDVTKVQAWQHRLNPLQKKI
                     GDGCHLNRKIDDLVLGASFEITSLKRFHLPSVPKFIGHCYQGIAAKPLSS"
BASE COUNT         3787 a         2751 c         2883 g         3902 t
ORIGIN      
        1 atcttcgctg cggatgcccg ccataataaa aatcagtccc gagatgaaga aacaaatcag
       61 tccaattagt tcaaaataaa atattttcat tgcattaatt tatgaaggac tctgtacgaa
      121 tagagaattg attgaaaata tcaccaaacc ggcccaaatc agtccgaacg tgatgctgtg
      181 agttggcgtg aacggttcat ggtaaagata cacaccaatt aagagttgga tagatggagt
      241 catgtattga ataaaaccca gggtagtata acgaagtcgt tttccggcat ttgtgaacca
      301 gagcagtggt agtgaggtga ccacgcctgc accgactaaa aaaatcacaa ttctccaatc
      361 tcctccaata ttccctgtac catctacatt ccaaaccact atcataactg ctgcgaaagg
      421 agcaagcagt aatgtctcca tcaacagacc caccaatggt tttactggta tcattttgcg
      481 tattaaaccg taaaaagaaa aggtgaatgc caacgataga gcaatccagg gcagtgagtc
      541 aaaatcccag agaaaattca aaacgcccaa agcagccatt ccaagtgcta aactttgcca
      601 ataattgagg cgttccttca aaaaaattgc tcccaataaa acattaaaca agggattgat
      661 aaaatatccc aaactggttt cgacgatttg attcgtgttt acaccataaa tataaacaaa
      721 ccagtttact cccaggagca ctgctgttcc caggagcatc cagatatatt ttggggtctt
      781 cagtaaatcc cgaaattccc ccaaacgctt ctgaactgcc agcaacccca ctaggaaaag
      841 cacggaccaa accattcggt gtgctaaaat ttctaacgaa ggaacggtgt caagaagttt
      901 ccagtaaatg ggaagaaaac cccatgcgct aaaagccaac agggcatatc cgattccaat
      961 ttgtgcttcc gtatgctgag atttaataaa attactcaag tagtaattta agtttcagga
     1021 ttcatgtttc agtctgctgg atgccggaaa gtgcagcgtg aacgtactcc ccttaccgta
     1081 tttgcttttc aatgaaactg aacctccaag caatttcatt aaactttgca cgagcgcaag
     1141 tccaagacct gtccccttta gtgttccgga tgagttgact tgatggaaga cttcaaatat
     1201 ttgctgctgc tgatctacgg taattccgca gcctgtgtct tggatttcaa ctgaaacacc
     1261 tgttgcatca cgaaagctcc ggactttgat gtgacctccg gattcagtaa atttaatggc
     1321 attacccagc agattgatca atacctgccg tacctttccg gaatcagaat gcagtaaaat
     1381 tggtgagtca gagaggaaaa caagtttatg ctgcagagat tttttctgca tcagaggttc
     1441 aagccattcc atcacatcct gcagtaaatt ttccaactga aactctgagt taaatatttg
     1501 catctggcct gcttctattt tggaaagatc gagcaatgaa ttgatcagtt ccaaaagatg
     1561 ttgcccgctt gttaaaattc tactgaggcg gtcaatttga taatctgaaa gctgctcctc
     1621 tcttcctgat tcaagcaaga cttccgcaaa gccgattata gcattaagcg gagtacgcaa
     1681 ctcatgagac atgttggtga ggtacatacc tttggcccgg ctggcttgat caagctgacg
     1741 atttgaggtt tccagtaatt cagtacgttc gcggatgacg gactccattt tttgaaaaac
     1801 ttctgtcaac gtttgaactt catccatatt ttccggaaat tcatctgctt tgccctcgac
     1861 ttcaatggat ttttcagcag gcttgtggaa ctgatcacgg ataagctgct gagccgcacc
     1921 ccgtaatttt ctaagtgggg cagtgattcg gtttgcaaaa taaactgcca atcccaaaac
     1981 aagaagtaaa actacagaag caacgaccca gatccagatt ccaagtcctg acaaaccttg
     2041 ccgcaattca gattcctgct gacgtgcaaa atcggtttgc cagcgaatca ttatttccac
     2101 ctgactgctg atgttctgta ccaaaggcga ctccttgtct gtccagataa tctgtgcttc
     2161 ttccagagta agtttttgtt ccggatcgct tgtgtttttt tgcagatttt caagggataa
     2221 aatcatcaac cgcaaatcat aaaaaacgcg tctctcaact gatcgttctc cttcccacac
     2281 acgtaccttt ttgtaaagca ggtcgagttg cttaaaggca gggtgaatat caactgccca
     2341 tgtaaggtca cgttctttaa tgaacttcaa atctccgctg tcgatccagg ccttttgtgc
     2401 cagtgaggct ttatggactg cattattcag cgaaaaccac ttaagggaaa ctgcagggag
     2461 atcacggtgt ctgtccaaag actgatgctg ttccaatgta agccagtaca gggcaacaaa
     2521 acctaacagg gttattgatg cacaaactgc tgcaaccaga ataattttat ggcgtaaact
     2581 ttggaacatt tttgttacag catgaagaca ggattttaga tttgggataa tttaataaca
     2641 atgttttaca ggtttactcg acagtcaaga gttttatcgt gtcgtcccga acgtatgtga
     2701 gggatcttat aagatatcag cactttatca gatctctccc ttcgctcgaa atgacatgac
     2761 gcagtgaata atatcagtat cttactagaa taaaatactc attagtagga ttgtagcaaa
     2821 cgatttagac tctacaattt gataaaaata agcaacaaag ctcttaaaaa ttagaacata
     2881 aatattgctc attatcgagt gatttgtcct gtaccagatg aaattcatca agccttcagc
     2941 ttgcaagatt tatgtaaaca gggcactctc aaaatgcaac gtttaacgtt tttattttca
     3001 gaaagattat gaaaaataag tccatccagc agcgttttca tgcagctcag gtgttggtcc
     3061 gtgacgcagg tctttatgct cttcagtggt ttagaaaact tgatagtctg aagacggaaa
     3121 aaaaaggacc tcaggacttg gtaagcaaag cagatcgtga agttgaaaaa atgattcgtg
     3181 aacgtctgca gactttattt ccggaagatg ggtttttagg tgaagaaagc ggaaccagaa
     3241 atttggatgc agaaggtatc tggattgttg atcctattga cgggacaatc tgttttctaa
     3301 atggtttgtc atcctggtgt gtatcgattg cctttgttgt taagaaccaa attgagatag
     3361 gtttgattta cgcaccgtgt acggacgaat tgtttgcatc tcagcgtgga actggtgcta
     3421 ccttaaacgg taaaccaatg aaaccgagta aggccactac acttgccgat ggaattgtcg
     3481 ggctgggata ttcaccaaaa agttcgattg ttgcaaccca aaaagccttc gattatttgt
     3541 ttaaaaatgg tggtgtttat cacgacgttg gatctgcagc tttgatgtta gcatacgtag
     3601 ctgccggacg ttacattggg gtttatgaat ttcaaattaa ttcatgggat tgtctggcag
     3661 gtatcgctat ggttcgtgaa accggtggtt ggacaaatga ttttctggcc aatgatggtt
     3721 tactcacagg caatccagtc ttagttgcag ctccgggagt gaaagacgcc atgcagaatt
     3781 tatttgctgc cgccggtcat taattccatc ctgtttttta gttcagccaa tgtttcagtc
     3841 aattcgacct ttgccaaatc cttcgatggg agggcattgg cgtggaaatt cgtttcaatt
     3901 cggattgatt gctgagcatg ttgtcagcat ccgctatttg ttttacccac atttctttcc
     3961 gcaacttgaa ggaaatcagg ccttgctgtc cgatgtccag ttggacacat cgtatcagtt
     4021 tggtgatgta tggggctggg aagctgaatt ttccggctct gcagaagaac acatggttta
     4081 cctgattcag cttcaagaaa aggacggcaa cacaaaattt gtttttgatc catttgccag
     4141 ggaaagcttc ggtggtgagc attgggggag acccattgga tttgttgtgg atgaggagga
     4201 ttttcggctc agtgttattt cccgtcccaa aagtaaggtt tttcatggaa tgcgccggtt
     4261 gcccgttctg cgtcaggaaa caccgacaga tgaattaact gcttcccgtc cacaccgccc
     4321 tcatcaccct ctggagcaga gtgtcattta cgagtgccac gtccgtggca tgaccaatag
     4381 cccatcaatc tcagtttccg gatcttcaca atccgggaca tatcggggac ttgttgaatg
     4441 tattccgtat ttaaaagcac ttggtgtgac tgcaattgag cttttgccgg tttttgattt
     4501 tgatgaaaac gagaggatta ttactgatgc ggaaattgag catactgttc taaactattg
     4561 gggttactca ccgcttttgt tttttgcacc aaaacaaaat tatgcgtcag atgtggtaaa
     4621 tccagttcgc gaatttaaag cgatggttga caaattccat gaagctgacc tggaaatctg
     4681 gctggatgtt gtttttaatc atactgcgga atttggtgat aatggttatg cagatcattt
     4741 taagttactg gcgccggatc agtggtattt gcgggaaaag gacggaagct acaaaaatta
     4801 ttccggatgc ggaaatactt tgaattgtgc tcatccaaca accagacgaa tgattcgtga
     4861 ctgtctcatt tactggtcac acgttatggg tgtggacggt tttcggtttg accttgcttc
     4921 aattctaaat cgggattcgg ctggagacat tatggacttt ccgcagttac tctgggaatt
     4981 aagacatgaa ccttccctga aaaatattaa actgatttca gaaccatggg atgccgcagg
     5041 tgcttacgaa ctgggaaaag cagctgagtt tgcagattgg acagaatgga acgatcaatt
     5101 ccgtgacaaa gttcgtcgtg ctgtgcgtgg agatgagggc atgatggaag ggcttaaaga
     5161 gtcaattctg ggcagtccaa atatttttgg ttcagtagaa aaaggcagga agcggagtct
     5221 aaactttgtt acttctcatg atggtatgac tttgatggac ctggtttcat acagcgaaaa
     5281 acacaacgag gcgaacggcg aagaaaaccg ggatggccac agtgctgatt tttcggacaa
     5341 ctgcggaact gaaggagtaa ctgatgatcc ggaaatactg tcattgcgct tccggaagtt
     5401 acgcatgttt cattgcctgc tgcagctttc caacggaatt cccatgctgt tgggtggtga
     5461 tgaattcggg aggacacaac agggcaacaa caacgcttat tgtcatgact ctgctttaac
     5521 ctggctggac tggaatctga cagaacaaaa ctccgtgctt ttagctttta ctcggcgtat
     5581 gattgccttt cgcaaacagc acgctccatt tttattttca gaaaaaagta aatataaatg
     5641 gtttaatgct tcaggtggac cggaggagtt tgcaccctac gtccggacac tacactttga
     5701 agtaacaaat cctggttggc cagaccaggc aatgcgcatt ttgataaatt gttttgacaa
     5761 gccggttaaa tttaatctgc ctgaagaaat agcctggaac gttctgattg ataccgaaaa
     5821 ggaacctgct gaattcgtct cgccggttgg agggattgcg tggcttgagg gtttttcagt
     5881 acaagttttg aggggacact cagttgatgg ttgacgtttt caaaccaaaa attaaacttg
     5941 tctcagagga gatatatgcc aaaatcaaaa aaacatactc ccgcattggg agcggattac
     6001 agcggaaaaa tgaagagccg ctttgctgaa aaggttaaac cgtctggcaa agtatattca
     6061 aaagcagatc gtcgtgataa tcgagtaaaa gataatttgt cagaggaact gcaacaggaa
     6121 attaaaaaag agaattcaat gcagggcgga tgtttgtgcg gtgcagttaa atttaagctg
     6181 acgggtgaat tacgtccggt tatcaattgt cattgcgggc aatgtctcca tactcatgga
     6241 aattttgcgg cctatacgtc tgtggaaaaa aagaattttc agcttgttaa tgatgttgga
     6301 ctgaaatggt ttcgctcctc aaacgaagca agacggggat tttgtcagga atgtggtgcc
     6361 agtatttttt ttgaacgttt aggcggcagt aatatcagca ttgctgcagg gatgttggat
     6421 tcttcaaaag gtctaaaaac tgttgaacat atttttgttg atgacaaacc tgattattat
     6481 gaaattgagg atgatttgcc aaaattttct caatactaca aaagacaact tgaaagcgat
     6541 acttaacaat aaataatatt ttttattttg atggtttcgt caaaagtctg caatccactc
     6601 cgcacctaaa tcagcaatct ggaaatgaaa aaatagaata atccaggggc tgttaaggtg
     6661 cggggtcatt tcgaccgcag ggagaaatct taatgatatt aatgtaggag cttatacaag
     6721 atttctcgct tcgctcgata tgacactgtt ttacgacttt ttacaggttc atctaatttg
     6781 taggataaaa atcacaaaat caacatgcag tctccatagc tgaaaaaacg gtatttttcg
     6841 gctacggcgt gctgatagga acttaataaa aattcgcggc cacaaaatgc tgaaactaac
     6901 agcatcaatg ttgatttggg aaggtgaaaa tttgtgagaa gcccatccgt cattgtgaat
     6961 ttgcagggtg gataaataaa aatgtctgtc caacccgagc tttcttgaaa attctgccga
     7021 actaaggttt ccaggaccct cacagaagtt gtgccgacat tgatcatctg tttgttattg
     7081 ttttggtaga cttgcagttg acgtagtgtt tgagaagaaa ctgaaaaaaa ttccgcatgc
     7141 attttgtgtt ttcgaatatc ttcttgatca attgaggaaa aggttcccaa accgacatgc
     7201 agagttacct ccaaaatatc gattccacga tttccaattt ctgttaaaat ttcaggcgta
     7261 aagtgtaagc cggcagtggg tgctgcaatt gcgcctggtt cggtagcaaa acaggtttgg
     7321 taacgttctc gatcttttgt attctgcaaa tcacttgcgt ttcgtcgctc aatgtaaggt
     7381 ggcagcggcg gcagaccaac atcttccaat cgctctttca aacgttctgt ttcctcaaac
     7441 tccaaaagcc attcaccctc ttccagccgt tcttttgctt ttgccaaaag ttttccatca
     7501 caaaattcaa gacgctcacc ctgtttaatt cgtccagctt ttcggactat tgcgctccag
     7561 tctccgttgt tattttcttc aatcagcagg gcttcaattt ttccaccgct ttgacgtttt
     7621 cccggaatgc gggcaggtaa aactttggtg ttgttgataa caaggcagga ggattcaggg
     7681 agcagtgaaa caatttcagt aaagttgcgg tttgaaattt ttccgctttt acgttcaaga
     7741 aaaagcatgc gtgactggtc acgttttggg gcaggatgct gggcaatgag ggattgagga
     7801 agcttatagt cgtatgaact gagcttccaa tcgtgagagg gcatttggat tatgagaatt
     7861 aaatcaccag actggaggca gcacttgatg gttgtttctt agaaaaaata gtcctgttgt
     7921 cggctgctaa cttgttttat ccataccgtc catttgtttg atccgctctt caggagaaat
     7981 aatattcgtt aattttgcac caaacttttc attgacaatt ataacttcac cttgagcaat
     8041 caacttaccg ttggcaaata aatccaggct ttcaccaact aaacggtgca attcaagtac
     8101 ggaaccctgt ccgagtgaaa ggaggtcgtt aatggtcatt tttgcacgtc ccacttcaag
     8161 ggtcattgtt aaaggcattt tctgtaaaaa ttccaggtca acaccttctt caaccggtgt
     8221 atcatccatg gacgaggtgg tgtctgaagc tgagtcaagc atttcgtcga tattttcctc
     8281 tgttgaatct gctttttccg ttattaaaga actaaggtca ccgctgcctc ctgtaccttc
     8341 aggacttgtt ttctgctgct ctaactcatt catcaacgaa tccatatcgt catcagggct
     8401 gtctccattc tcggtttcag ctgctttcat ttctgcgttt tcctgctctt cctgaacttc
     8461 ttcgtctacc atttttactc catttaataa atttattgga aggaaagggg taactccagt
     8521 tcccagcatt ttctcagttt cggttcttcg agtgcatcac atccggaagg gctttcaaag
     8581 tctttcaact taaaagaaaa tgctaattca tccggaatat tattttgcga tacagcggaa
     8641 cgtttattaa tctcaaatgc tgcagtacac ctaaatttat cttctcttaa tttttccagg
     8701 ttttttattt tcacctgaca atccaattgc tgaacactgc aataatgctg aacatgagct
     8761 tcagcaactt gttccagttc aggcaaaaaa attgaacccc gataagctga accataaact
     8821 tgtccttgtt ttcgactctc taaaaacctt gggatctcat ccggagtcat acggccatat
     8881 actcgtccgc ttggaaaatc aatcattgtt ggtgcaaaac gatgccctcc aaggtgactg
     8941 ctctcccaaa cttcaacaga agaattttgc ttttgaacat gatattgcat ctgatcagca
     9001 agtttctgtc caaacttggc acagcattta tcatgcctcc catgggtaca aataaaaatc
     9061 tgctcccgct caatcttttt acaggaaaaa tcggaagttt tgtaatgctg aaaatgtgac
     9121 tctaaaactt ctggtatttc tttgggcaga acgtttgaat aataccattt tccgggatag
     9181 ataaataatt ccactttgtc attttcgagc tttgcctgac tggctaaacg gatactgatt
     9241 tttccgctaa ttttactttt ttctttcatc cattgtttta gtccagttgg gaatccgcct
     9301 ttggattcca gagcttcgta ttcccagtat tttttgggcc aggaaataaa aacaaaatgc
     9361 tctgcaaatg gggcagtgcc cgcaagaggt tctcctgaat gtctgcacaa aaaactgcaa
     9421 aactttgaat cggacatttc tggttatctg gtttaagtgt taatagtgtt tcaaactgaa
     9481 acaacagaat ctaagggttt gtaacaggca actttatgaa caatttcagc aggatttttg
     9541 tcagattcca ttgccgaact attattcaga tttaaaagtg acggagattt ttctctgcat
     9601 ttttcctcta caagcggaca acgggaagca taggcgcatc ctccttcagt ttcgggaaaa
     9661 agttgggttg acgctgattt ttttaaacgt cctgtgcgtt tccttgacgg gtcaggacgt
     9721 ggaactgctt caagcagagc tctagtgtat ggatgttgag catgatcaaa cagggtttcc
     9781 gttggacctg tttctacaat tcttcctgca tacatcacag ccacatttgt agtcatgtga
     9841 cgaacgatgc tcaggtcatg actaataaaa agataagcca gtccatgctg ttcccgcaag
     9901 tccatcatta aattcaatac ctgagcctgt actgacacat ccaaagccga taccggttca
     9961 tccgccacaa tcagtgacgg acgagtgatt aaagcacggg caattgcaat tctctggcgc
    10021 tgtcctccgg aaaactcatg cggaaatttg ttcgcgtctg caggtgagag tcctacgttt
    10081 tcaagggctt cagcaacctg atgctgctgt attttcagtg ttacttctct cagcaatgta
    10141 aggggctcag atactatttg acttactgaa tggcggggat ccaacgagcc gtatggatct
    10201 tgaaaaacca tttgcattcc acgacgcata cgtcgcaaat ctttggtttt cagggtgtgc
    10261 agaaccttac cttgaaaaca aacttctccg gactgaggag attcaagtgc taatacagtt
    10321 ctggccaaag tagatttccc gcaaccggac tcgcccacaa tgccaaaact ttcaccgtaa
    10381 tttagcgaca aactcactcc atcaagtgca cggaagcgtt ttggaggaat aaaaatatgc
    10441 tggcgcggca ggatatattc cctcactagt tcgcggattt ggagcaaagt atttgcattc
    10501 ttttcctgtt tcatgcgttt tgctcccagg ggaaaaaaca ggctgcggaa tgtccgttac
    10561 ttatccgggt ttcatccgga agctctgaaa aacattttgc ctgtgcccgt gaacagcggt
    10621 ctgcaaaaga acagcctttg gtgcgttccc agaattccgg aacacgtccg ggaattgttg
    10681 acaacctgct atttttggga ttttcaggat gaaggctaag atcattagca gacttttctg
    10741 tgtcaggaat tgaggcaaaa aggccgcgtg tgtatggatg tgccaaatgt tcaaacactt
    10801 tatttgttgg acctttctct acaatccgtc ctgcgtacat taccagcatt tgatcagtat
    10861 tttctgcaac aacacctaaa tcgtgtgtta tcagcatcag tgccatgttt tcctcaacaa
    10921 ccaattcaga aatcaaagcc agtatttgtt cctgaatcgt gacatccagt gcagttgtag
    10981 gttcgtctgc aataatcaat tccggttcac ataccatagc tatcgcaatc actaccctct
    11041 gtctttgtcc tccggaaagc tgatgtggat aaagtgagcg cgaaaatttt ttggcaggta
    11101 agccgacgcg gtccaacact tttgaagcct gtcgctgcgc ctgatgtcgg gataaatttt
    11161 tgtgcagaat caaactttct gctacctgga atccaattgg ctggactgga tttagtgccg
    11221 tcattggctc ctgaaaaacc atactgatac ggttccccct gattttacat aattcctttt
    11281 caggtagatt cagcaagttt ttaccgaaca taacttcgcc ggaggcagtc atattttccg
    11341 gcaataaacc catgattgca aaggccgcca ttgatttccc gcaaccggat tctccaacta
    11401 ctcccaagct tttgccgctt tcaagtttga aattcaggtt gtccaaaatg ttaattggac
    11461 cagattggtg ttctgtacga acagttaaat tattaacggt caataaattc aaatggttgt
    11521 tgctggatgt atgtattttt gtaaaatgtg aaaattttca ataaatgcga aactgcatgc
    11581 tgcagagaaa agcagctaaa ttgtttttct ggatggcaag ctttttggaa agcttatcct
    11641 atcacggcag tttttgcatt cagaaattga ccattttgtg taatctgcta tttattataa
    11701 cagaaaagtg taaattcatt agcaatctta tgattgtaga aagatattta tatgcccatt
    11761 ttacaaagtg aaacagcaaa ggaatgaaac tatgaggtca gctattcaag aagaattatt
    11821 tggtcctgaa caatacaccc gtttatgcag catgtgccgg ggggaatttc cccgttcgcc
    11881 ggaatttttc cctccaggac gatgtcagga cagactggct tcgttttgtc gtaaatgtgc
    11941 taatgtccgg gcacaggttc ggcaagctac caaagaaacg gaaagaaggt tgtctcaaat
    12001 gaatttgctg gaaaagagct gtgaaggatg tggaacaata aaatcattga gggaattcta
    12061 catgtcttca cattcacatg atggcaaaac gtcaacctgc aaaaactgca tagatgcaaa
    12121 gagcagtgaa agaaagatgc gtcagcagcg tcttggagat ttggcctggg cagtttattt
    12181 tatccaggat tcacgcaata acagagttaa aataggttca tgtgatgatc cttatgtggc
    12241 actggaaact ctgcaaaaag gatcttcaga aactctgcat ttgctggcaa gtcatgaagc
    12301 aggacaaaaa gagtctgcgg aaaatattaa aagtacgctg cacagtttgt ttcaaactca
    12361 ctacacagga cacggatggt ttgaaatggt gccttcgctg gaacagtaca tttcccttat
    12421 tcagcaggaa gattacgaca aggcggaaat tctactccat ccaatggaaa acactcaaaa
    12481 tgaagctgct caaaagacag tcccaaaaga catcacagaa ggagctgtgt ttgttgaggg
    12541 acagaaattc cccagtattg cagcagcatc caacgcaacc ggatattctg ctgaacagtt
    12601 gaaaaaatac atccaaaatt ctgaggaatc cggtatctcc gttacatcaa aactcaaaaa
    12661 agcagtttga ttggtgaatc agtttcgagg cgccatgtcc ggaagttttt attcaaaatt
    12721 tgtatttccc tacctttgtg aacttaccat gtccggaaag tcactgagtg gttttcggca
    12781 ggatgcactt caggaaataa acggggaagt tctggaaatc ggttttggaa caggactcaa
    12841 cctgcctcat tttccggaaa ctgtacagaa aattgacgca gttgatgtca acccccgaat
    12901 gcaccgtctg gcacggaaac ggattgataa gtccggaatt gcagtgacaa attatgttct
    12961 ttccggagag catttgccaa tggatgatgc aagttacgat tatgttgtct gcacttttac
    13021 cttgtgcagt attccggatg tgtcacaggc gttgcttgaa ataaaccgtg tgcttaaacc
    13081 acgagggaat tttgtgtttt tggaacacgg gttgagcgat gtcacgaagg tacaggcttg
    13141 gcagcaccgt ctcaatcctc tacaaaaaaa aattggtgac ggttgtcatc tcaatcgtaa
    13201 aattgatgat cttgtccttg gtgcttcttt tgaaatcact tcattgaagc gatttcatct
    13261 tccgtctgtt ccaaaattca ttggacattg ctatcagggt attgcagcca aaccgttgag
    13321 ttc
//
LOCUS       DTVD01000003           13231 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1027_length_13231_cov_325.875835, whole genome
            shotgun sequence.
ACCESSION   DTVD01000003 DTVD01000000
VERSION     DTVD01000003.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1027_length_13231_cov_325.875835"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="EYO68_00090"
     CDS             complement(<1..661)
                     /locus_tag="EYO68_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HIC06237.1"
                     /translation="MIIGVLKEIHPGERRVAMAPSVAKQCIKNGHSVLLEHGAGIIAN
                     FTDDQYEDSGVEIVESAHKIWEKTDVILKIRPPEETHETEEHEADLLRKGACLICLLN
                     PGRNSDLLKRLAKTGGTAIAVDAIPRISRAQSMDVLSSMANISGYRAVTEAAHNFGRL
                     FTGQITAAGKIPPAKVLVIGAGVAGLSAIGAARSLGAIVRAFDTRTSVREEIQSMGAE
                     YL"
     gene            884..2887
                     /locus_tag="EYO68_00095"
     CDS             884..2887
                     /locus_tag="EYO68_00095"
                     /inference="COORDINATES: protein motif:HMM:PF00773.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIC06238.1"
                     /translation="MLESLTNHYCIYFIDHKMEFGWIEGIQKNKLIIVPPQGKTQFLL
                     PNRVAYSWREKKLPFNAAQAHETLELHMKQAEQYKQTFELETMHSLLENMREYTLEEL
                     AVDFLDKPENSVSKLGLFLALREDSFWFKHNRNLTYTPRTSEELALLEVQFARLQEQQ
                     KRAVNIQKWIKQLESGEWNANTKITAEQQNWLDQQLNLLIEGTESPYWKEMSTLLDWG
                     TSFGIGEENSLKRWLAGAGTPVSTSRLTLLRANVREEFPEKVLADVERVRKLTSAKLT
                     RSPAEMQTFTVDAESTLDYDDAFSVLEWNKAQLIVAVHITDLSHSVHPGDPLFKEAED
                     RISSVYTIEETIPMLPDELSNDFFSLMSGEERAVLSFKFKLNGNGDWSLLEVIPRMIK
                     VHENLSYEQADLLIENNHDFWGLLNKFCKCAQERRLEKGALNLARKEFDFDISDPEQI
                     RIIPLKRNSPASRIIEELAIAVNRETARLFQEADFPGIYRTQSPYELIKEVEDGELLS
                     LENIRIEPAKLTTVPGIHSGLGCEVYMHATSPIRRFADLITQSQLKKLIKKEEPVFST
                     EDMMHWAEEVSFRQRKYNKAERNITQYWKLRYLQQHLGEIYVAKIRKRLPNNNTEIEL
                     LELACVVPAAGLGKNEEGELMLRIDEVGLDPPRIGVHIQTLST"
     gene            complement(2921..3925)
                     /gene="fliM"
                     /locus_tag="EYO68_00100"
     CDS             complement(2921..3925)
                     /gene="fliM"
                     /locus_tag="EYO68_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIC06239.1"
                     /translation="MGSVLSQEEVDALLQGVTSDEEVSEEDEEEYDPEEVVSFDLTAQ
                     DRIIRGRMPTLEIIHDRFVRLFRLTVSNALRRVVDISVRSTELIKFGEYIKTLPVPTS
                     MNLFRMTPLRGNAMMVFETRLVFTLVEMFFGGSGEVETKSEGRDFTEIEARMIKRVII
                     SGLEDLQTSWRPVFPVQVNYARTEVNPQFVSIVPHSEIVVVVTFDIEIGRAPMSITVC
                     VPYSMIEPIRTRLNAGFQSEQDEKDNTWGNRFKQNLQKVDIELIAKLGEINISVRDFL
                     NLQMNDVLYLEHETKDPISVEVNGVKKFTGFQGAYKGHKAINIDELIYEPPVSDEMLV
                     "
     gene            complement(4062..5354)
                     /locus_tag="EYO68_00105"
     CDS             complement(4062..5354)
                     /locus_tag="EYO68_00105"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIC06240.1"
                     /translation="MTEKAKIILPDGQTMDLPVLTGSENEKGIDISGLRKQTGYITLD
                     PGFVNTGSCESSITFLDGEKGVLQFRGYPIEELAEESTFIEVAYLLINGELPKKDQLE
                     NFTNRINLHSMIHEDMRHFFEGFSQNSHPMVILSAMISSLSAYYAEASGKASIENLEI
                     NSARLIAKISTIAAFSYKKSVGQPFVYPRDELPYCAKFLNMMFSVPAESYELDPVIEQ
                     SLELLLILHADHEQNCSASTVRVVGSSMANVYASVASGILALWGPLHGGANQQVVEML
                     QQIHEDKAGISKYIEKAKNQDSSFRLMGFGHRVYKNFDPRAKILKVACDKLLNKLGIN
                     DPLLEIALELEETALTDDYFIERKLYPTLDFYTGIMYRAIGIPLNMFTVMFALGRVPG
                     WIAQWKEMHEDPKQKIARPRQLFTGNTRRSYTTITERH"
     gene            complement(5387..6310)
                     /gene="argF"
                     /locus_tag="EYO68_00110"
     CDS             complement(5387..6310)
                     /gene="argF"
                     /locus_tag="EYO68_00110"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIC06241.1"
                     /translation="MKRDFISILDWTSEEIRSNLDFAVELKHQTKEGKCPQLLQGKTF
                     GLFFHKPSLRTRLSFEVGITQLGGTAIVLTEQDFKIGERETVSDVARVMSRYVDCILI
                     RTFSHQMVLDLSKHATVPVVNMLTDFNHPCQVMSDMLTIKEKLGRIDDVRIAYLGDSN
                     NMTNSWLNIAARIPLDLRIGTSPETLPDMELVRKIQEIGISSIKVTHSATEAVDGAEV
                     LYTDVWASMGEKDKIAERERLLKEFQINSSLLQHAEKNALVLHCLPAERDKEITDEVI
                     EGRQSVVFDQAENRLHAQKAILVQLEKWNQV"
     gene            6511..7269
                     /locus_tag="EYO68_00115"
     CDS             6511..7269
                     /locus_tag="EYO68_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HIC06242.1"
                     /translation="MSLKLPLRLIYPVIVGILWLTGCASLSGTVQSGVSPSQFENLSK
                     SKGNYYVSGRILFKHPEGKHSGEMEIHISADSELRLSIFTPLVGSLIYELRVGPEKFL
                     LLNFQEKNYVLDENNRYVRQTWLGMDLSLTELKWLILGQIPEKTPAWQRTKLPSGELQ
                     LVQGTSVIRLRLNSAGRIQSMNKSVEGLLEYKAQISLYQKHYEILFPRKIRIEDFTGN
                     NHWLMVISGVQAPTGMETLDFIPPEDMKLLISNQ"
     gene            complement(7263..8042)
                     /locus_tag="EYO68_00120"
     CDS             complement(7263..8042)
                     /locus_tag="EYO68_00120"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06243.1"
                     /translation="MSILDVPDPDGFLYIVADSHLDEKNAPAEEFVEMLVQLENPHTI
                     VFLGDLFKIWLAPPKFWSDLHRQVLLGFQSLKDKGCNVVFIAGNREMLLPGKFTDNWK
                     KKLPFTHLIHNDWFLNWGNQHFGFIHGDTINYHDRQYLRWKSVSHSLAVETIFQLMPG
                     PAARWIAERIEAMLVETNKEYKVHFPEKEIQEFAESVLPGVDNYYVGHFHVDREIKVD
                     GCSSVLRVVPDWLSQRKLIRVSAEGTKEVLHFQNGSFENAH"
     gene            8224..9024
                     /locus_tag="EYO68_00125"
     CDS             8224..9024
                     /locus_tag="EYO68_00125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:PRK11880.1-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIC06244.1"
                     /translation="MRLIIIGGGNMGGAILLALVKAEVIPPKNILLIEPDDAKRQNLS
                     KATSCDSQAEFNENVRNYDLLILAVKPQGATPAMELLAQWLLPHQLVISVMAGISLQT
                     MHGALGQSKLVRVMPNIPTLIAEGMSVFFASENVDSDGIKWVKTLFSSCGTCLEAKNE
                     DAIDAATAISGSGPGYLFYFAEQMTESAKSLGFTEEEAQLLVQKTILGATLLWESQQV
                     SPETLRKQVSSPGGTTLAALNHFQSENVGESIKEGIQQAYLRAKELTS"
     gene            9057..10349
                     /locus_tag="EYO68_00130"
     CDS             9057..10349
                     /locus_tag="EYO68_00130"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIC06245.1"
                     /translation="MACVIIVGTQWGDEGKGKIVDLLTERADAVVRYQGGNNAGHTVM
                     FGEKTFILHLLPSGILRKTTSILGNGVVIDLAEMIKEIDELAQMDIAVEDHLHISDRA
                     QLVMPWHKTFDRLSEEQKGKNKIGTTGRGIGPAYEDKVRRSGMRVGDLLEPELFKARL
                     EEIVEEKNKLLELYYKSDEPSFSAEEIFSEFTAYLKILKPYIRDTPLLVNQMIEEGKN
                     ILFEGAQGTFLDVDHGTYPFVTSSNTLAGGACAGTGIGPTRINEVLGIVKAYTTRVGS
                     GPFPTELLDNDGELLQSEGHEFGATTGRPRRCGWFDALLVRQAVRLNGITSMAVMKLD
                     VLDKFDTLKIAVSYRLSNGEHTENLPRSLENVTPVLEEMPGWKCKSAGITEYDQLPRE
                     MLAYLERISELVGAKVSIISTGPKREETIVLNPDQFWL"
     gene            10377..10601
                     /locus_tag="EYO68_00135"
     CDS             10377..10601
                     /locus_tag="EYO68_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06246.1"
                     /translation="MFKRIILVCLIAVIAYYAGAQGLTPGNVVDWFTENDIFQTSKEA
                     LKEIFELAEEQQVVEKAGGVIDSLKEKVTN"
     gene            complement(10619..11983)
                     /gene="murF"
                     /locus_tag="EYO68_00140"
     CDS             complement(10619..11983)
                     /gene="murF"
                     /locus_tag="EYO68_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIC06247.1"
                     /translation="MTKLYTIGEMSQIFNAKTEKIKTDLLVHGFSIDSRTMNAGELFF
                     CIKGENTDGHHYISQALEKGACAVVADPACIPEAIRQSSFPRILVPDPNLALREWAAD
                     IRKRFTGKVLAVTGSNGKTTTKEILAGLCRHLDPNAYATPGNFNNFIGVPLTLLEAPP
                     DTKWWVIEIGTNHFGEVAELSKVVQPTAGIITNIGQSHLEFLQNTEGVAREKSGLFAG
                     MSPGNKVIVPDTLLHLDLVEEEAVKAGVDIVKTTVVTEQASEKNCKIRFFNEWFESSI
                     DSPLMQQNLVLALTLLKEEGVPVALLKQAAAALDLSVKGRYQQIEMDDWILIDDTYNA
                     NPSSFYSVLENLNKTYPGLRKIVVCGAMAELGDLSPELHRQVGETMVKNGVEKMFGLG
                     GAEIKFYLEGWKNGGGKTEAAQHFSDIDDLTKTFREELRASDVVLVKGSRSAQMERFV
                     EAML"
     gene            12020..13096
                     /locus_tag="EYO68_00145"
     CDS             12020..13096
                     /locus_tag="EYO68_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIC06248.1"
                     /translation="MDIERQFEEVFSKITLDNGQPLVSVEPEIVHGCVVNNDFAKITL
                     ILPEDSPLRGSLPAQVEAEIKKIEQIERVAIEVLASPPEEKTASQTTQAQTEPQQPKR
                     TAYLQNYEAVIAVASGKGGVGKSTVSVNLALALSQKGLKVSLFDADIYGPSLPIMTGK
                     RGEKPSVVGNRLVPLESFGMHTMSIGNLVDENDPVIWRGPMVHQAIEQLLRDTDWPGG
                     DIMIIDMPPGTGDTQLSISQLCELTGTVIVSTPQDVALIDAVKAAKMFTKVEIDVLGI
                     VENMSFFACPKCGEETPIFSRYGAEETSKKIQAPFLGAIPIELEVRKGGDDGKPLMAS
                     NLDSPASMAFKNIADNLVKVLEEG"
BASE COUNT         4000 a         2722 c         2656 g         3853 t
ORIGIN      
        1 ccaaatattc tgcccccatg ctctgaattt cttcacgtac ggaagttctt gtatcgaaag
       61 cacgtactat tgcacctaaa ctccgggctg ctcctatagc agacagtccg gcaacgccgg
      121 cgccaatcac caaaacttta gccggaggga ttttccctgc tgccgtaatt tgccctgtaa
      181 agagacgacc aaaattatgt gctgcttcag ttactgctct ataaccagaa atatttgcca
      241 tggaactaag tacatccatc gactgcgcac gtgaaattct aggtatagca tccactgcaa
      301 ttgcagtccc accagttttt gcaagccttt tcaataaatc ggaattgcga cctggattta
      361 acaggcaaat caaacacgcc cccttacgca gtaaatctgc ctcatgttct tcggtttcat
      421 gagtttcttc aggaggacga atcttgagga ttacatctgt cttttcccat attttatgtg
      481 cagactcaac tatttctaca ccagaatctt catattgatc gtctgtaaag ttagctatga
      541 ttcctgctcc gtgttcgagc aggacagaat gcccattttt aatgcattgt ttcgcaacag
      601 aaggtgccat cgcaaccctg cgttcgcctg gatgaatttc tttgagaact ccaatgatca
      661 ttagttactc ctaaatttgt ttgaggtgaa tgtaagcccc tttaatataa catagagtta
      721 aattcatctg cataggaaaa tttacgaaaa aaatagtaga tgtaatctta gaaagtgttt
      781 tttaacaaaa catgctttta ggacttggag atattcgctc ataaatagta cactacagac
      841 cgaacctaga ttattacaat cagcttttct caaattcaaa caaatgctgg aatcattgac
      901 aaaccactat tgcatttatt tcatagatca taaaatggaa tttggatgga tagagggcat
      961 acaaaaaaac aaactgatca tagttccgcc gcaggggaaa acgcaattcc tgcttccaaa
     1021 ccgtgtggcc tacagttggc gtgagaaaaa attgcctttt aacgctgctc aggcacacga
     1081 aaccctagag ctgcacatga aacaggccga gcagtacaaa cagacctttg agctggaaac
     1141 aatgcattca cttttggaaa atatgagaga gtatacactt gaggagttag ctgttgattt
     1201 cctcgataaa cctgaaaatt cagtcagtaa actgggttta tttctggctc ttcgtgagga
     1261 ttcattctgg ttcaagcata acagaaattt aacttataca cccagaactt cagaagaatt
     1321 agcattgctg gaagttcagt ttgcccgcct gcaggaacag cagaaacgtg cagtcaatat
     1381 ccagaaatgg attaaacaac ttgaatccgg agagtggaat gcaaatacaa aaataactgc
     1441 agaacagcaa aattggctgg atcaacagct taatttgtta attgaaggta ccgaatctcc
     1501 gtattggaaa gaaatgtcca ccctgttgga ctggggtaca tcgtttggaa ttggtgaaga
     1561 aaattcactc aaacgctggc ttgcaggagc aggaactcct gtctccacat cccgcttaac
     1621 acttctgcgt gcaaatgttc gagaggaatt tcctgaaaaa gtgttggcgg atgttgaacg
     1681 tgttcggaag ttaacatcag caaaattgac aagatcacct gcggaaatgc aaacctttac
     1741 agttgatgca gaaagtaccc tggattatga cgatgcgttt tctgtgctcg agtggaataa
     1801 agcgcagtta atagttgcag tacacatcac tgatttaagt cattcagttc atccgggaga
     1861 tcctctattt aaagaagctg aagaccggat ttcctccgtt tatacgattg aagaaaccat
     1921 tcccatgtta cctgacgaac tttctaatga ctttttttcc ttgatgtccg gtgaagagag
     1981 agctgtgctt agttttaaat ttaaactgaa tggaaatgga gattggagtt tacttgaagt
     2041 tattccaagg atgatcaagg tacatgaaaa tttgagttat gaacaggctg atttattaat
     2101 tgagaataat catgattttt ggggattact gaacaaattt tgtaagtgtg cccaagaacg
     2161 gcgccttgaa aaaggggcat taaatttggc aagaaaagaa tttgattttg acatcagcga
     2221 tcctgaacaa atccgcatta taccgctcaa acgaaacagt cctgccagta ggattattga
     2281 agagcttgca attgcagtta atagagaaac tgcacgtctt tttcaagaag cagactttcc
     2341 tggaatttat cgaacccagt ccccgtatga gttgatcaag gaggtggagg atggagaact
     2401 gctttcgctg gaaaatatcc ggattgaacc agcaaaatta acaactgttc caggtataca
     2461 ttcagggtta ggctgtgaag tttatatgca tgcaacttca ccaatcagaa ggtttgcaga
     2521 cttaatcacg caaagtcagt tgaagaaact gataaaaaag gaggagcctg ttttcagtac
     2581 cgaagacatg atgcactggg ctgaagaagt cagcttccgt cagagaaagt acaacaaagc
     2641 agagaggaac attactcaat actggaaact aagatattta caacaacatc tgggagaaat
     2701 atatgttgca aaaataagaa aaaggcttcc caacaataat actgaaattg aattattaga
     2761 acttgcttgc gtggtacccg ccgcaggttt ggggaaaaat gaagaaggtg aacttatgct
     2821 gagaattgat gaggttggat tagatcctcc ccggattggt gttcatatac agacattgag
     2881 tacgtaagac tcctctcagc agtttcaaag caacagcctt tcatacaagc atttcatcgg
     2941 acacaggagg ttcataaatt aactcgtcaa tatttattgc tttgtgacct ttataggcac
     3001 cttgaaatcc ggtaaatttc ttgaccccgt ttacttcaac cgaaatcgga tcctttgttt
     3061 catgctccaa atacaaaaca tcattcattt gtaaattcaa aaaatcacgc acagagatat
     3121 taatttcacc cagttttgca attaattcaa tatcaacttt ctgcaaattt tgtttgaacc
     3181 gattgcccca ggtgttatct ttttcgtcct gctcactttg gaatccagca ttcagtctgg
     3241 ttcgaattgg ctcgatcatc gaatacggta cacaaactgt aatagacatg ggtgcccgcc
     3301 cgatttcaat atcaaaagtc accacaacga caatttcact gtgaggtaca attgagacaa
     3361 attgcggatt gacctctgtt cgggcatagt ttacctgaac tggaaaaaca ggtctccatg
     3421 atgtttgtaa atcttcaagc ccactaatta ttacgcgctt gatcatccgt gcttcaattt
     3481 cagtaaaatc acgtccttct gatttagttt caacttcgcc actgccgccg aaaaacattt
     3541 cgactaaggt aaaaaccaac ctggtttcaa agaccatcat ggcattacca cgcaacggtg
     3601 tcattcggaa gagattcatg gaggtaggaa caggcaaggt tttgatgtac tcaccaaatt
     3661 taatcagttc tgtggatcgc acactgatgt ccaccactct gcggagtgca ttggatacag
     3721 ttaaacggaa gaggcgcacg aaacgatcgt gaataatttc tagagtaggc attctcccgc
     3781 gaataatgcg gtcctgagca gtcaggtcaa aagataccac ctcttcaggg tcatactcct
     3841 cctcatcttc ttcacttacc tcttcatcac tggttacccc ttgcaacagc gcatcaactt
     3901 cttcctgtga caaaacagaa cccatgttcg ccctttttat ttaatatttg agtttaatta
     3961 agtaattgca ccaaggtctg tggtctcagc acatgacaag gctaactatg caagtagtgt
     4021 tcaactaaat taaatgttgt tttatacttg tcacctgctg cttaatgacg ctcggttatc
     4081 gtcgtgtagc tgcgcctagt attacctgta aacaactgac gcggacgggc aattttctgc
     4141 ttgggatctt catgcatttc cttccactgg gcaatccatc ccggaacacg acccagagca
     4201 aacatgacag taaacatatt aagtggtatt ccaattgccc gatacataat tccagtatag
     4261 aaatctaatg tgggataaag cttacgctca ataaaataat cgtctgtaag tgcggtttcc
     4321 tcaagttcaa gagcaatttc aagcagcgga tcgttaattc ctaatttatt cagtaacttg
     4381 tcacatgcaa ctttgaggat cttggcgcgt ggatcaaaat ttttgtaaac ccgatgacca
     4441 aaacccatga ggcgaaaact agaatcctga tttttggctt tttcaatata ttttgaaata
     4501 cctgctttgt cttcatgtat ttgttgcagc atttcaacga cttgctgatt agcaccacca
     4561 tgcagaggcc cccataaagc taaaattcct gaagcaacag acgcataaac attcgccata
     4621 ctgctgccga caacccgtac tgttgaagca ctgcagtttt gttcatgatc tgcgtgtaaa
     4681 atcaagagca actccaaaga ttgttctatt accggatcta attcatatga ttctgccgga
     4741 acagaaaaca tcatgtttaa aaattttgca cagtaaggca gttcatctct tggataaaca
     4801 aaaggctggc ctacactttt tttgtaggaa aatgctgcaa tagtactgat ttttgcaatt
     4861 aaacgggcag aatttatctc caggttttca attgatgctt ttccagatgc ttctgcatag
     4921 tatgcagaga gtgaggaaat cattgcagaa agaattacca ttggatgtga gttttgggaa
     4981 aagccttcaa agaagtgtct catatcctca tgaatcattg agtgcaagtt gattcgattg
     5041 gtaaaatttt ccagttgatc ttttttgggc aactctccgt ttatcagcag gtatgcaact
     5101 tcgataaaag tagactcttc tgccaactcc tcaatgggat aacctctaaa ttgcaaaacg
     5161 cctttctctc catccagaaa agtaattgaa ctctcacagg aacctgtatt gacaaatcct
     5221 ggatccaaag tgatgtatcc ggtttgttta cgtaaacctg aaatatcgat ccctttttcg
     5281 ttttctgagc cggtcaaaac cggcaagtcc atagtctgac cgtcaggaag aataatttta
     5341 gctttttctg tcataaaacg aatttatgct ggtggtgaaa atatgctcaa acctgattcc
     5401 atttttctaa ttgaactagt atagcttttt gtgcatggag cctgttctct gcctgatcaa
     5461 aaactactga ctgtcgtcct tcaatcactt catcagtaat ttccttatcc ctttcagcag
     5521 gcaggcaatg taaaacgagg gcattttttt cagcatgctg caacaggctt gagttaattt
     5581 gaaattcttt taataaacgc tctctttcag caattttatc tttttctccc atactggccc
     5641 aaacatccgt gtacaaaact tctgcaccat ccacagcttc tgttgcagag tgtgtaactt
     5701 tgatggagga aattccgatt tcttgaattt tacgtactag ctccatatcc ggcagagttt
     5761 ccggagatgt tccaatacgt aaatccagtg ggattctagc agcaatattc agccaggaat
     5821 tggtcatatt attactgtct ccaagataag cgatgcggac atcatcaatg cgtcccagtt
     5881 tttccttgat agtcagcata tctgacatga cctggcatgg atgattgaaa tccgtaagca
     5941 tattgaccac cggaactgtt gcatgttttg ataaatccag caccatctga tgtgaaaaag
     6001 tacggatcaa tatacaatcc acataacgtg acataacccg tgcaacgtca cttacagttt
     6061 cccgctctcc aattttaaaa tcctgctctg taagaacaat tgcagttccg ccaagttgtg
     6121 tgattccaac ctcaaatgag agtcgtgtcc gtaaagatgg tttatggaaa aacagaccaa
     6181 aagtcttacc ctgcagtaac tgaggacact tcccctcctt tgtctgatgc ttcaattcaa
     6241 ctgcaaagtc aagattgctt cttatttcct cacttgtcca atccaaaatt gaaataaaat
     6301 ctcttttcat atttataaaa aataacaggt tctaataaaa tttattgcat ctgcagaaaa
     6361 taatagttag catgaattct aacattatag aaaccacttg ttaagaggaa tcatgcatga
     6421 aatgaatatt ctagtcacag ttgctgtgat tgcaggcctt tacatctccc tctttctcgt
     6481 tgcagagaaa gtatttatga aagacgaaga ttgagtctca aactcccatt gaggctcatt
     6541 tatccagtca tagtaggcat tctttggtta acaggatgcg catccctttc cggtactgta
     6601 cagtccggag tttcaccctc acaatttgaa aacctgagta aaagcaaggg gaattactat
     6661 gtttccggaa gaatattatt taagcatcct gaaggcaagc acagtgggga aatggaaata
     6721 catatttccg ctgattcaga attaaggctg agcatattta ctccgctcgt cggttcatta
     6781 atatatgagt tgcgagtggg acctgaaaag tttttgctgt tgaattttca ggaaaaaaac
     6841 tatgttttgg atgaaaataa ccggtatgta cgtcagacat ggctggggat ggatctttct
     6901 ctgactgaac taaaatggct gatcctggga caaattccgg aaaaaacccc agcctggcaa
     6961 cgaacaaaat taccttccgg agagttgcag ttggttcaag gtacttcggt tatacgtctt
     7021 cgcttaaatt ctgcaggacg gatacagtcc atgaacaaat cagtggaagg tttgctggaa
     7081 tacaaagcac aaatttcact ctatcaaaaa cactacgaaa ttctgtttcc cagaaaaatc
     7141 cggattgaag attttactgg caacaaccac tggttgatgg tcatcagtgg agtacaggca
     7201 ccaaccggaa tggaaacact tgatttcatt cctcctgaag atatgaaact cctgatcagt
     7261 aatcaatgag cattctcaaa tgaaccgttt tgaaagtgca gaacttcctt agtgccttct
     7321 gcgctcactc tgatgagttt acgttgtgaa agccagtcag gaacaactct caaaacgctt
     7381 gaacatccgt caacctttat ttctctatca acgtggaaat gccccacata ataattatca
     7441 actcccggaa gcacagattc agcaaactct tgtatttctt tttcaggaaa atggactttg
     7501 tattctttat tggtttccac caacattgct tcaatccgtt cggcaatcca acgcgctgca
     7561 ggacccggca tcagctgaaa aattgtttca acggccagac tatgtgaaac agatttccag
     7621 cgcagatatt gtcggtcgtg atagttaatt gtatcaccgt gaataaaacc aaaatgttga
     7681 ttgccccagt tcaagaacca gtcgttgtga atcaaatgtg taaaaggcag cttctttttc
     7741 cagttgtctg taaatttgcc tggtaatagc atttccctgt ttcctgcaat aaacaccaca
     7801 ttgcatcctt tatctttcaa cgactgaaaa cctaacagta cttgacggtg caggtccgac
     7861 cagaattttg gaggtgcgag ccaaatttta aatagatccc ctaaaaatac gatggtatgt
     7921 ggattttcca gttgtaccag catctccaca aactcttcgg caggcgcatt tttctcatct
     7981 aaatgtgaat ctgcaacaat atatagaaaa ccatccggat caggcacgtc taaaatggac
     8041 atctattata atcctatttc aaataatata actgtatatc aggtatttag caaataattg
     8101 caatctaaat attctcttat aatattagat aatctactgg ttttttaata aaattaaaag
     8161 tataatattc agggtttgat tatctcaatt cattatttaa aatctataga ataatagaag
     8221 tccatgcgtt tgataattat tggtggtgga aacatgggag gagcaattct cctggctttg
     8281 gttaaagcgg aagtaattcc tccaaaaaat attttgctga ttgaaccgga tgacgcaaaa
     8341 agacaaaatt tgagtaaagc cacaagttgc gactcccagg cagaatttaa tgaaaacgta
     8401 cgaaactacg atctgcttat attagcagta aaaccacaag gtgcaacacc agcaatggaa
     8461 ctgcttgccc agtggcttct accgcaccag ctcgtaattt cggtaatggc aggaatttca
     8521 ctgcagacta tgcacggagc cctggggcaa tcaaaactgg tgcgtgtaat gcccaatatc
     8581 cctactttga ttgcagaggg tatgagtgtc ttttttgctt cagagaatgt tgattcagat
     8641 ggaatcaagt gggtaaaaac tcttttttct tcctgcggca cgtgtttgga agcaaaaaat
     8701 gaagatgcaa tcgatgcggc caccgctatt tccggaagtg gtccgggcta tttgttctat
     8761 tttgcagagc aaatgactga atctgcaaaa tctcttggtt ttacagaaga agaggctcaa
     8821 cttcttgtac aaaaaactat tcttggtgca acgctgttgt gggagtcaca acaggtgtct
     8881 ccggaaactt tgcgtaaaca ggtttcatct cctggtggaa ccaccttagc agctcttaat
     8941 cattttcagt cagaaaatgt tggtgagtcg atcaaagaag gtatccaaca ggcttatctg
     9001 agggccaaag aacttacttc ataatttcat aataaattca aattctaata actaatatgg
     9061 cttgtgtaat tattgtaggc actcagtggg gtgacgaagg aaaaggtaaa attgtagatt
     9121 tactcacaga acgtgcggat gcagtagtgc gttatcaggg tgggaacaat gcaggacata
     9181 cagtgatgtt tggggaaaaa acattcattt tgcatttgct tccttctgga atattacgta
     9241 aaacaacttc cattcttggt aatggtgttg tcatcgattt ggcagagatg atcaaagaaa
     9301 ttgatgaatt agcgcaaatg gatattgcag tagaagacca tttgcacatc agcgacaggg
     9361 cacaattggt tatgccctgg cacaaaacct ttgaccgcct tagtgaagaa cagaaaggca
     9421 aaaataaaat tggtactaca ggtcgaggaa taggtcctgc ctatgaagat aaagttcgac
     9481 gatccggaat gcgggttgga gatctgctgg aaccggaact gttcaaagct cgcctggagg
     9541 aaattgttga agaaaaaaat aaactgcttg aactttatta caagtctgat gaaccttctt
     9601 tttctgcaga agaaatattt tcagaattta ctgcatatct caagatcctt aaaccataca
     9661 tccgtgacac gccgctgctg gttaatcaga tgattgagga aggaaaaaac attttgtttg
     9721 aaggcgcaca gggtacattt ttggatgtag atcatggaac ttatcctttt gtaacttctt
     9781 ctaacacttt ggccggagga gcctgtgccg gcaccggaat tggtccaaca cgtatcaatg
     9841 aggtactggg gattgtcaaa gcctacacca ctcgggtagg aagcggccct tttccgactg
     9901 aactgcttga taatgatggt gagcttttac agtctgaagg acatgagttt ggtgctacaa
     9961 caggacgtcc ccgtagatgt ggctggtttg atgcattact tgtacgtcaa gcagttcgtc
    10021 ttaatggaat taccagtatg gctgtaatga aactggacgt gctggataag tttgacactc
    10081 tcaaaattgc agtgtcttat cgattatcaa atggagagca tactgaaaat ctaccaagat
    10141 cgctggaaaa cgttactccc gttttagaag aaatgccggg ctggaaatgt aaatctgcgg
    10201 gtatcacgga atatgatcag cttccccggg aaatgctggc ttatcttgag agaatttctg
    10261 agttggtagg agcaaaggta tctattattt ctacgggacc gaaacgtgaa gaaaccattg
    10321 tactgaaccc tgatcaattc tggctctgaa aatcttataa tgctgggtat gaaaagatgt
    10381 ttaaacgaat aattttagtt tgtctcattg ctgtaattgc atactatgca ggagctcaag
    10441 ggctgactcc aggaaacgtt gtagattggt ttacagaaaa tgatattttt cagacctcaa
    10501 aagaggcact gaaggaaatt tttgaactgg ctgaagaaca acaggttgtt gaaaaggctg
    10561 gtggagttat tgacagtttg aaagaaaaag taaccaactg aataaccatt gaattacatt
    10621 aaagcattgc ttcaacaaag cgttccatct gcgcggatcg tgagccttta acaagtacaa
    10681 catctgacgc ccgtaattct tccctgaaag ttttcgttaa atcatcaata tcgctaaagt
    10741 gttgtgcagc ttcggttttt cctcctccat ttttccagcc ttcaagataa aatttaattt
    10801 ctgcaccacc aagtccaaac attttctcta ctccattctt taccattgtt tcaccgacct
    10861 gacgatgtaa ctccgggctt aaatcgccta attcagccat ggcaccacaa acgacaattt
    10921 ttcgaagacc tggataagtt ttgtttagat tttccagcac actgtaaaaa gaagaaggat
    10981 tggcgttata tgtatcatca attaagatcc aatcatccat ttcaatttgc tgatacctgc
    11041 ctttcactga caaatccaag gctgctgctg cctgcttaag aagagctacc ggtacccctt
    11101 cctctttcaa aagcgtcagg gctaaaacca ggttttgctg cataagaggg ctgtcaattg
    11161 aggattcaaa ccattcgtta aaaaagcgta ttttacagtt cttttccgat gcctgttctg
    11221 taaccactgt tgttttaaca atatctacgc ctgcttttac ggcttcttct tccaccaaat
    11281 ccaaatgcag caatgtgtca ggaacaatga ctttatttcc aggtgacatc cctgcaaata
    11341 atcctgattt ttctctggca actccttctg tgttctgcag aaactcaagg tgactttgac
    11401 cgatatttgt gatgattcca gcagttggtt gaacaacttt tgaaagttct gcaacttcac
    11461 caaaatgatt tgtaccaatt tcaattaccc accattttgt atccggaggt gcttccagca
    11521 aggttagagg tacaccaata aagttgttaa aatttccagg agtcgcatat gcattcggat
    11581 caagatgacg acataagccg gcaagtattt ctttggtagt tgtttttcca ttacttccgg
    11641 ttacagccag cacttttccg gtaaaccgtt tacgtatatc tgctgcccat tcccgcaaag
    11701 ccagattggg atccgggacc aaaattcgag gaaaactact ttgacggatg gcttccggaa
    11761 tgcaggcagg atctgccaca accgcacagg cccctttttc cagtgcctga gaaatgtaat
    11821 ggtgtccatc agtattttct cctttgatgc agaaaaacaa ctctcctgca ttcatagtac
    11881 gagaatctat gctgaatcca tgcaccaata aatctgtttt aattttctca gttttggcat
    11941 taaatatttg cgacatttca ccaatagtat ataattttgt catagctttt tatttacaat
    12001 caataaggat aaaaaataca tggacataga aagacagttt gaagaagtat tttctaaaat
    12061 caccttagat aacggccaac ctttggttag tgttgaacct gaaatcgtac atggctgcgt
    12121 agtcaataat gattttgcaa aaatcacgct tattttgccg gaagattctc ctttacgcgg
    12181 atcactacca gcacaagttg aagcagaaat aaaaaaaatt gaacaaatcg agagagttgc
    12241 cattgaagtt ttagcttcac cgcctgaaga aaaaactgct tcacaaacta cacaagctca
    12301 aactgaaccg caacaaccta aacgtacagc atacctgcag aattatgaag ctgtaattgc
    12361 tgtagccagt ggaaaagggg gagtcggtaa atctactgtg tctgtaaatt tagccctcgc
    12421 actttcacaa aaaggtttaa aagttagtct gtttgacgca gatatttatg gaccaagttt
    12481 acctatcatg acaggtaaac gtggcgagaa accatctgtg gtaggtaacc gcctggttcc
    12541 actggaatct tttggcatgc ataccatgtc aatcgggaac ttagttgatg aaaatgatcc
    12601 cgttatctgg agaggaccca tggttcacca ggccattgag caactgctgc gtgacactga
    12661 ctggcccgga ggagatatta tgataataga catgcctccg ggaacaggtg acactcagct
    12721 ttccatttct cagctttgtg aactaactgg aaccgttatc gtttctacac ctcaggatgt
    12781 ggctttaatt gacgcagtca aagcagcaaa gatgtttaca aaagtagaaa tagatgtttt
    12841 aggaattgtc gaaaatatga gttttttcgc ctgtcccaaa tgtggtgagg aaacaccaat
    12901 tttcagccgg tatggtgcag aagaaaccag caaaaaaatt caggcaccat ttcttggtgc
    12961 tattccaatt gagcttgaag tacgtaaagg cggagatgat ggtaaaccgt tgatggcaag
    13021 caatttggac tcaccggctt caatggcctt taagaacatt gcagataatc tagtgaaggt
    13081 tttggaagaa ggttaattct tcattcaatc taaatagtgc caattattta ttgacttttt
    13141 tactctctct gctagtctta aatgtccctt gcgggtcggt agctcagctg gtagagcatc
    13201 tgacttttaa tcaggtggtc cggggttcga g
//
LOCUS       DTVD01000004            3475 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10365_length_3475_cov_427.074269, whole genome
            shotgun sequence.
ACCESSION   DTVD01000004 DTVD01000000
VERSION     DTVD01000004.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_3475_cov_427.074269"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="EYO68_00150"
     CDS             <1..824
                     /locus_tag="EYO68_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06249.1"
                     /translation="SEQILLHNLRKELARTYDFSDQSSFEQALQKLRQSTNKQSCLQL
                     KCILKILKNFPQTSLFLLKSIPRETRLTLVLIGENRKWRVKHEACLECRLTQQDMLKS
                     IVLRMGSYSVHPTAVLDLKPEQPSKSSSPEYSLKKEESDQKIKTEVTNLIPEPKISES
                     EIVPARKAMLPLELLKFKIAQRRYNQLIWKNIKKDLMFFRQKHRNRSSRNLKTRLRLQ
                     IDQFGKVIERRLLKTSGSQKFDKIIMDSVDQLKLPPPMELLIRHPPYVVTILIQP"
     gene            837..1826
                     /locus_tag="EYO68_00155"
     CDS             837..1826
                     /locus_tag="EYO68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HIC06250.1"
                     /translation="MEVILTEDQNSVLKTQENRALEVKQPVLEPGEALNLVFGEYAAN
                     TQRAYARAFRDLQQWANIRELQELENFDPLRMLEYKNQLKASGKKPSTINQTMSALRK
                     VCKVLTEFGYLKENPFKASVIRAEKVDSAASKGALQVSQLNAMIEANEVIHYDGRIAP
                     LLRFRNGLVLKFLYLTAARRGEAADLLWEDIVQDGSFHVAVLRHTKSGKEQRIKLRNE
                     LHAEFEEWHKMLQANKIAAPNVFPSLGFRTLGHKMSGKGINDIVARLGKAIGLNISAH
                     YLRHTAITLALELGEPLQKVQSYARHASANTTIRYFHDRQLLEKNPTDSLPMI"
     gene            complement(1891..3285)
                     /locus_tag="EYO68_00160"
     CDS             complement(1891..3285)
                     /locus_tag="EYO68_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIC06251.1"
                     /translation="MSKAYRIEKDSMGEVKVPQEALYGAQTQRAVENFPVSGICISRP
                     LIHALGVIKQGAAKVNAEMDNIPKDVAHAIQLAAQEVIDGKLDEHFPIDIYQTGSGTS
                     SNMNANEVIAHRAMELVPELKVKVHPNDHINFGQSSNDTFPTAIRIAGFLEAKNTLIP
                     ALKFLRETILKKGAEYSHVVKTGRTHLMDAMPVTFEQEFSGYARQIEIGIQRIESALL
                     QMAELPMGGTAVGTGINTDPEFPPKFAAEISKLTGESFREAENHFEAQSTVDAPVAMG
                     AQLKTLAVSLLKIVNDLRWMNSGPNGGLGEIQLAALQPGSSIMPGKVNPVIEESMAMV
                     CAQVIGYDAAISIGGLSGNFELNVMLPMVAHSLLESIRLLSNASRNLAARSVSKLVVR
                     EDHIKALVGKNPILVTTLNPLIGYDLAAKIAKKAFAENRALKEVALEMCDLSEAELDK
                     ALDPTKMTKGGFVE"
BASE COUNT          971 a          790 c          748 g          966 t
ORIGIN      
        1 tttcggaaca aatcttgctt cataatttac ggaaagaatt agcccggact tatgatttca
       61 gtgaccagtc ttcctttgaa caagcccttc aaaaactgag acagtcaacc aataaacaat
      121 cttgtctgca actaaaatgc atcttgaaga tcctgaaaaa ttttccgcag acaagcttgt
      181 ttttgttgaa aagtatacca agggaaactc ggcttactct ggttctgatt ggagaaaacc
      241 ggaagtggcg tgtaaagcat gaagcctgtt tagagtgcag attgactcag caagatatgt
      301 taaaaagcat cgtgcttcgg atggggagtt attccgtaca tccaacggca gtgctggatt
      361 taaaaccaga gcaaccgtct aaatcatctt ctcctgaata ttcattaaaa aaagaagagt
      421 ctgatcaaaa aattaaaact gaagttacaa atttaattcc tgaaccgaaa atcagtgaaa
      481 gtgaaatagt tcctgccaga aaggcaatgt tgccgctcga actgctaaaa tttaaaattg
      541 cgcagcggcg ttacaatcag ttgatctgga aaaatattaa aaaagattta atgttctttc
      601 gacaaaaaca ccgtaatcgg tccagcagga acttaaaaac acgacttcgt ctgcaaattg
      661 atcaatttgg caaggtcatt gaacgacgac tgttaaaaac aagtggttca caaaagtttg
      721 ataaaattat aatggattct gtggatcagc tgaaactgcc tccgccaatg gagcttttaa
      781 ttcgccatcc accatatgta gtaacaattc tgattcagcc ttgaaattta tattaaatgg
      841 aagtaatttt aacagaagat caaaattctg tactgaaaac tcaggaaaat agagcgctgg
      901 aagttaagca gcccgttctt gaaccgggag aggctttgaa tctggttttt ggtgaatatg
      961 cagccaacac gcagcgtgct tatgcacggg ccttccggga tttacagcaa tgggcaaata
     1021 tcagggagtt gcaggaactg gaaaactttg acccactccg tatgctggaa tacaagaatc
     1081 agttgaaagc ctctggtaaa aaaccatcta caattaacca aaccatgagt gccctgcgca
     1141 aagtctgcaa ggtgctgaca gagtttggct atctaaaaga aaatcctttc aaagcctctg
     1201 tcattcgggc agaaaaagtt gactctgcgg ccagtaaagg cgcactccag gtttcgcagt
     1261 taaatgctat gattgaggca aatgaagtta ttcattatga tggacgaatc gcgccgctgt
     1321 taagatttcg caatggtctt gtgttgaaat tcctgtatct gactgcggca agacgcggcg
     1381 aagcagcaga tttgctttgg gaagatatag tgcaggatgg ttcctttcac gtcgcagtac
     1441 tgcggcatac aaaaagcgga aaggagcagc gtatcaaatt gagaaacgaa ctgcatgcag
     1501 aatttgaaga gtggcacaaa atgcttcagg ctaacaagat tgcagcacca aatgtttttc
     1561 cttctcttgg tttccgtaca cttggtcaca aaatgtcagg aaaaggtatc aatgacattg
     1621 tcgccagact tggcaaagca attggcttaa atatttccgc ccactatctt cggcataccg
     1681 caatcacact tgcactggag ttgggtgaac ctctgcagaa agtccagtcc tatgcccgac
     1741 atgcctccgc caacaccaca atccgctact ttcacgatcg gcagctcctg gaaaaaaatc
     1801 caactgacag ccttccgatg atttaaaaag acgctctctt ccagatcatt ctcctactgg
     1861 ccgatcggaa aaattgatgg aagctgattt ttattccaca aatccgcctt tggtcatttt
     1921 tgtcggatca agcgctttat ccagttctgc ttctgaaaga tcacacattt ccagggcaac
     1981 ttctttgaga gcacgatttt ccgcaaaagc ttttttggca atttttgctg ccagatcata
     2041 accgatcagt ggattgagag ttgttaccag aattgggttt ttgccgacaa gcgccttgat
     2101 gtgatcttca cgcacaacca gctttgaaac ggaacgggca gcgagattcc gggatgcgtt
     2161 cgacagcagg cggatggact cgagcagact gtgcgccacc atcggcaaca tcacattcaa
     2221 ctcaaaattt ccggacaaac cgccgatact gatcgctgca tcgtaaccga taacctgcgc
     2281 gcagaccatt gccattgatt cttcaatcac cggatttacc tttccgggca tgatcgaaga
     2341 tcccggttga agtgccgcca gctgaatttc tccgagcccg ccgtttggtc cggaattcat
     2401 ccagcgcaaa tcgttgacaa ttttcaagag actgactgcc agagttttta attgggctcc
     2461 catggcaacc ggtgcgtcca ccgttgattg tgcttcaaaa tgattttccg cttcccgaaa
     2521 tgactcaccg gtaagttttg aaatttctgc cgcgaatttg ggtggaaatt ccggatcagt
     2581 attaattccg gttccaacag cggtcccgcc catcggcagt tccgccatct gcagcaatgc
     2641 tgattctatg cgctgaatac cgatttcaat ttgccgtgca taaccgctga attcctgttc
     2701 aaatgtcacc ggcattgcgt ccatgaggtg tgtccggccg gttttgacca catgcgaata
     2761 ttcggcccct tttttcaaaa ttgtttccct caaaaattta agtgccggaa tcaaagtgtt
     2821 tttggcctca agaaatcccg caatcctgat ggcagtcgga aaagtgtcat ttgaactttg
     2881 accaaaattt atgtgatcat tcgggtgaac cttgaccttc agttccggaa ccagttccat
     2941 agcgcggtgc gcaattacct cgttggcatt catattactt gacgttccgg atccggtttg
     3001 ataaatgtcg atcggaaaat gctcgtccag cttgccgtca atcacttcct gcgccgcaag
     3061 ctggatcgcg tgtgctacat cttttggaat gttgtccatt tcagcattaa cttttgctgc
     3121 accctgtttg attacgccca gtgcatgaat cagcggacgg ctgatgcaga ttccgctgac
     3181 cggaaagttt tcaaccgcgc gctgggtttg cgctccataa agtgcctcct ggggaacctt
     3241 gacttctccc attgaatctt tttcaatgcg gtatgcttta ctcattttgt tcctttcttt
     3301 tagtttgttg tggttatttg tttttgttta ttcatcacgt tgtttggtga aaatattttt
     3361 gaaacaaata tactatttat ttttgtcagg ataaaaacgt aatatcctct cattgtaagc
     3421 acacccctgg attcccgctt gcgcgggaat gacaactccc taccaagaca gtaga
//
LOCUS       DTVD01000005           13139 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1044_length_13139_cov_205.173341, whole genome
            shotgun sequence.
ACCESSION   DTVD01000005 DTVD01000000
VERSION     DTVD01000005.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_13139_cov_205.173341"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /locus_tag="EYO68_00165"
     CDS             complement(<1..2282)
                     /locus_tag="EYO68_00165"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03150.12,HMM:PF07593.10,HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06252.1"
                     /translation="MLIRGINISGLVLAVCFLLARPFASDGREIVKTFDLPGTGKVSV
                     WSEKLSKQKLSCSAGFITHNLDHYTSVDGDTVDQFEANGAGVALGDLDGDSDLDVVLG
                     NHSGTNTILWNQVTQNMDDGFFPNFISEHMSFGNTRAVNLVDVDADGWLDIVMTRRNG
                     AFNYLRNTGQPDYTDSFNGIQRASGTSFVQQVLPGIAWPAYALNWADLDLDGDLDLVT
                     GSYDASLLENQGNDFLIGNGAGVLIYINQEGKYVPNRLAEKAQAMAIAFFDINRDGSK
                     DIVVGNDFAVPDYAWLRMATTTSSGNINSKEKILEFPWSLQVNGWIPTSFDTTSYSTM
                     SLDVGDVDNDQISELYSTDMMPYDETDTTVTAYEPLMADMDHNRNAGDPQIMANVLLV
                     NTGVVGYQDAARPRGLDATGWSWSAKFGDLDQDGLLDLYVVNGMAESTVFAHLDNHEL
                     IEANQVFRNIGNGYFKPAPEWKLGSTFGGRGMSMGDLDGDGDLDIVVNNLRGPAQLLE
                     NRLCSGESLQVDLHWHNDSPLAFQPQMGEIRNTRAIGTVVYLKTSAGNFTRDVRVASG
                     YLSGDPPRLHFGFPTNTSLYSLEIHWPDNVVSIVTDLSPQTLLKVSRLSGFFRNSRIP
                     QKDSVVDQKKEEKDRNIKQAYPPKIECDALNRQSECLKVTNVLSEEKFDQQLRKIIAQ
                     HGLTGEPRNAHDMPSINEPLAQLGKKLFFSKALGGDLDSACASCHHPLLGGGDALSVS
                     IGVGAHDQDLLGSARTHPEGP"
     gene            complement(2457..3269)
                     /locus_tag="EYO68_00170"
     CDS             complement(2457..3269)
                     /locus_tag="EYO68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIC06253.1"
                     /translation="MKLLAIFAHPDDEVFSCGGTLARYADEGHQVSLICATRGEEGEI
                     VHPDMEPNSYPKGPERGLLRVDELAATCKALGISPPIFLDYQDSGFPIEVGLQNPQAF
                     MNADIFKIEEKILDRIGSIHPEIIITFDPHGMYGHIDHITIHRAALAAFWSAGSVMQP
                     APQRLYYPARTITQVEEMQRRFAGSSNSDLDPNIFGVSDDSICAQIDISRYADRKRLG
                     VYAHRSQFGTEEHINELLDGRPDFFRFETFVLGGARGGFPADRLNDLFDGVG"
     gene            complement(3404..4228)
                     /locus_tag="EYO68_00175"
     CDS             complement(3404..4228)
                     /locus_tag="EYO68_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC06254.1"
                     /translation="MYIKLRRGFLHLVLMLVGLIMLLPFLWMLSTSLKNPKEIFTYPP
                     IWIPSEIRWENYLDALTVVPFGRYYVNSIVVALSVTFIVLITSSLAAFAFARLKFRGR
                     EIIFFTYLATLMIPFPVLLIPNFLIVREFGWYDTYAALILPPAFSAVATFLYRQAFRG
                     IPTVLDDAARIDGASSWQIWWKVIMPNAGPVTAALGIFTFLGNWNEFLWPLVVTNRVE
                     MRTIPVGLSSFQGQYSVQWELLMSAAVIALLPIVIIYLFAQKWIIRGITISGMGGR"
     gene            complement(4383..5201)
                     /locus_tag="EYO68_00180"
     CDS             complement(4383..5201)
                     /locus_tag="EYO68_00180"
                     /inference="COORDINATES: protein motif:HMM:PF00528.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC06255.1"
                     /translation="MEKSTRFYSQWGRWSSRQQSEGYLFLLPSLLGFTAFVLIPVLIS
                     LVLSFTEWNLIQPIKFVGFENYYELLTADRIFLKVAGNTIIYVILIVPVQLLLGLILA
                     VALNQSIRGIIWYRLVIFMPVITNIVAVAIVFQFFLNRDFGIASGWIWQIAEVTGWAV
                     APPDWLNSQELSKSAVAMLTIWKNVGFSMVIYLAGLQGVPESLYDAARVDGAGNWQRF
                     FHVTIPMVSPTTFFLIIIQMIGAFQLFAEPYVLTRGGASAIITIRGSVHLSECF"
     gene            complement(5581..6969)
                     /locus_tag="EYO68_00185"
     CDS             complement(5581..6969)
                     /locus_tag="EYO68_00185"
                     /inference="COORDINATES: protein motif:HMM:PF13416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIC06256.1"
                     /translation="MSEEKIEVQNNISSRRKFITTAAVAGRVKEIQMKITGSLMKTLI
                     GVACGLLVCVGIAQAKTTVTWGMWGSPAEIKTHQRVADAFMASHPDIQIILWGQPWGD
                     YFTKLKTLWAAGDKEGIPDVLFLSPVVTYAGQGVLEPLDPFIKASGYDTSDYWPSLLD
                     FGKLKGTIYGLPRDIGIEVLYYNKDIFDEAGVSYPTNFWTWDDLKAAAKQLSKITTSG
                     RVQRYALGAEGGKYQLFLGQNRGTILDDMVNPTACTLSEPVAVEAISFFAGLMDEKLA
                     MRPSALSQAGGDAGVFQSGQAAMIIQNASRISAFNAAKMNYDVAAVPIPAGGQRSGTA
                     GGAAWTMSKYSDDKDTAWTFLSWLQSTEGGQMVYSTSGEILPALRSTAVSDAFLGINA
                     PPANRAAFIIEGNNAKKGRSGYFPNWGTLNGKVISPNLSRIWSGSEPVRSVLEQTCKE
                     VNSFLQSKGLGF"
     gene            7436..7579
                     /locus_tag="EYO68_00190"
                     /pseudo
     CDS             7436..7579
                     /locus_tag="EYO68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
     gene            7801..10893
                     /locus_tag="EYO68_00195"
     CDS             7801..10893
                     /locus_tag="EYO68_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIC06257.1"
                     /translation="MILDSQKKLYRTKIDQFIKRLEALILSQYELLEAEFANSKDPVP
                     FGERLRLQYQRIEEGQEWGQAWESAWFHLQGRIPEKWQEQEVVAHLDFNGEALIFSPQ
                     GDPLQGLTNGSVFGNVTRHTFILTPKAQGGEPVDLWVEGAANSIVGINQPSDPPRGTV
                     DRQGNYLGKVNRMRIARFHRELYALRLDMELLNGLLDTVPEFSTRHARILKSLVESTN
                     GFRDDPDRAGICREILAPLLAQPAHSSSPTMITVGHAHIDTGFLWPVRETIRKAGRTF
                     ANQLDLIERYPDYVFGASQPQHYQFVKDHYPNLYERIKKAVAEGRWELQGGMWVEPDC
                     NLISGESMVRQVMHGKNFFMDEFGVEVTNCWIPDVFGYSASMPQILKSAGIHYLLTQK
                     MSWSQFNRFPYTTFRWRGIEGTEVLTHFPPEDNYNSELQPCGLQKAERQFVEKSILDE
                     MLCPFGVGDGGGGPTAEMIERGLRQQNLEGVPKVQFGRADHCFARIAKQQDQLPVWSG
                     ELYLEMHRGTYTTQARTKRGNRLLEQSLRETEYLLALGKLKDYPIEWLDGIWKKLLIN
                     QFHDILPGSSIREVYEVTEREHAEALEDCARIRAEAADKILQHDAEGLTVINTLNVPC
                     TREIQLPEDWQGGLVDDSGNPIDMQQDSDGTITAQLQLAPQAICTLHREGRMALAQSV
                     QSLVMENDLVRYEFNEHGQLLSAFDKEMQLEMLAEGEPGNILTLYEDRPHDWDAWEVD
                     ISYEEMALETARGKRWTSTGQGMVRQGLKFELSIGQSTIKQKITLSQKSKALEFDTLV
                     EWQECHRMLRTAFPVNVHTDSATFDIQYGYVRRPTHRNTSWDMARFEVAGQKYADLSN
                     HERGVALINNCKYGHKVHENVLDLNLLRSPTYPDPDADLGLHEFSYCLFIHQHELVKS
                     GVIAEALQFNQPPLLFPGKASVELQPPVRVNGKGVGLEVLKRAEKENCWIFRLVERYG
                     QTGIAQIELLEFEARVQETNLLEWEDMGGLLTSPLTLKFKPFEIRTFKIRSGLRQGPS
                     "
     gene            11297..12628
                     /locus_tag="EYO68_00200"
     CDS             11297..12628
                     /locus_tag="EYO68_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HIC06258.1"
                     /translation="MQGNSLIKEARKRALDVLHNCITPHGFRASALAAGYPQIWARDS
                     MIVSLGATTTGDPDLLAASRASLETMSTHQSSRGLIHLNVNPDTGYVSTENAGGTDSN
                     LWYILGHFLYQQASSDSDFLRSYWPQIERAMIWLGYQDINECGLLEVPEAGNWMDLLA
                     VRYNVLYDNVLYYAATLAYEKMSAQMALQADVQNLIDPAGIHQRINLLLWIERTWDAV
                     HFAEHLEKLKSMHLEWFMLYHNMGTISSRPYYMPWVAFREYGDWCDSFGNLLAILTGV
                     ADYNRSEQILCYMRQVGMTIPLPTKAIHPPIFPGDSNWREYYRSRNLNLPDQYHNGGI
                     WPMIGGFHVAALVSHGCQKKAESLLTLLAEANLTGISDGLEFNEWIHGKSGQPMGYAR
                     QAWSASMFLYADHAVHSGKLPLFDELQSAKPEVVRASEVNEVKYSGGGGPV"
BASE COUNT         3704 a         3107 c         2900 g         3428 t
ORIGIN      
        1 ggaccttctg gatgtgttct agctgaaccc aacagatcct ggtcgtgtgc accaacaccg
       61 atagagaccg aaagggcatc ccctccaccc aacaggggat ggtggcagct cgcgcaggct
      121 gaatccaaat ccccgcccaa agctttactg aaaaacagtt ttttgcctaa ttgagccaac
      181 ggctcattaa tggagggcat gtcatgggca tttcttggct caccagtcag cccatgctgg
      241 gcgatgattt ttcgcaattg ttggtcaaac ttttcttcgg acagtacatt agttaccttc
      301 aaacactcac tctgtctatt tagtgcatca cattcaattt ttggtggata agcctgcttt
      361 atgtttctat ctttttcttc ctttttctga tcaacaaccg agtccttttg cggtattctt
      421 gaatttctga aaaatccaga aaggcgtgag actttcagca acgtctgtgg ggataaatcc
      481 gtcactattg aaacgacatt atcaggccag tgaatttcaa gagaataaag tgatgtattg
      541 gtaggaaatc cgaaatgtaa ccgtggtgga tcacctgaga ggtaaccgct ggcaacacga
      601 acatcacgcg tgaaattgcc tgcactagtt ttcagataga caactgttcc aattgctcga
      661 gtgtttctaa tttcgcccat ttgaggttga aaagccaagg gtgagtcgtt atgccagtgc
      721 aaatcgactt gcaggctttc accactgcaa aggcggttct ctaagagctg agctggtcct
      781 cgtaaattgt tgaccacgat gtcaagatca ccatcaccgt ccaggtcccc catgctcata
      841 ccacgacctc cgaaagttga tccaagtttc cattccggtg ccggcttgaa atagccatta
      901 ccaatgttgc gaaagacttg gttagcttct atcagttcgt gattgtctaa atgagcaaat
      961 acggttgact cagccattcc atttacgaca tacaaatcta gaagaccatc ctgatctaaa
     1021 tcaccgaact tggctgacca actccaacct gtggcatcaa gtccccttgg tcgcgcagca
     1081 tcttgatatc caactactcc agtattaacc aagagaacat tggccatgat ctgaggatca
     1141 cctgcatttc ggttatggtc catgtctgcc atcaaaggct cgtaggctgt cacagtggta
     1201 tccgtctcat cgtagggcat catgtcagta gagtatagtt ccgaaatttg gtcattgtcc
     1261 acgtcaccaa catccaggct catggtactg taacttgtgg tatcaaagga agttggaatc
     1321 caaccattga cttgcagact ccaaggaaac tctaatattt tctctttact attgatattt
     1381 cctgaagagg tggtcgtagc catacgcagc caggcgtaat caggcacagc aaagtcattt
     1441 ccaacaacaa tatctttcga tccgtcccga ttaatatcaa aaaacgcgat agccatagcc
     1501 tgtgcctttt ctgccagccg attggggaca tatttacctt cttggttgat gtatatcaaa
     1561 acacctgcac cgttaccaat caggaaatcg ttgccttggt tctccaatag actggcgtcg
     1621 tatgagccgg taaccaagtc aaggtctccg tcaaggtcaa gatccgccca gttcaaggcg
     1681 taggctggcc aagcaattcc gggcaacacc tgttgaacaa aactagtgcc tgacgctctt
     1741 tgaatgccgt taaaactgtc cgtataatca ggttggccgg tatttcttaa gtagttgaac
     1801 gcaccgttac ggcgggtcat gacgatatca agccagccat ctgcgtccac atctaccaag
     1861 ttgactgctc ttgtattacc aaaggacatg tgttcactta taaaatttgg aaaaaaccca
     1921 tcatccatgt tttgagtaac ctggttccat agtatagtgt tcgtacctga gtgattccct
     1981 agtacaacat caaggtcact atcgccatcc agatcaccca aggcaacacc ggcaccatta
     2041 gcctcgaact ggtccaccgt gtctccgtct acagaggtgt agtggtcaag gttgtgagtg
     2101 atgaagccgg cagaacagga taacttttgt ttggacagtt tttcggacca tacactaacc
     2161 tttcctgttc cgggaaggtc aaatgttttt acaatttccc ggccatcaga tgcgaatggt
     2221 cttgctaaca ggaaacaaac tgccaaaact aatccactaa tgttaattcc tctgatcagc
     2281 attttattct tattcttccg gtttaactta attgtagtca ctcataaagc gataatcgaa
     2341 gcttaaagac gtcaattgga tctaaatgct gtatatgcct ttcaagtcgt caaatataaa
     2401 aatctgttgt aatcaactga aattttgaaa catcaaccat caattcctga gtattgttag
     2461 cctactccat caaagaggtc attcaatcga tcagctggga atcctccacg agcacccccg
     2521 agcacaaagg tttcgaacct gaaaaagtca gggcgaccat cgagcaattc gttaatgtgc
     2581 tcttccgttc caaactgaga ccgatgtgca taaacaccta aacgtttgcg atcagcataa
     2641 cgagaaatat caatttgagc acagattgag tcatcagata ctccgaatat gttagggtca
     2701 agatcggagt ttgaagaacc tgcaaatctc ctttgcattt cttccacctg agtgatagtt
     2761 ctagcaggat aatacagcct ttgtggtgca ggctgcatca cgcttcccgc tgaccagaag
     2821 gccgcaagag cagcacgatg aattgtgatg tgatcgatat gaccatacat accatgagga
     2881 tcaaaggtga tgatgatttc cggatgaata gaaccaatgc gatccagtat tttttcctca
     2941 attttaaaaa tatctgcgtt catgaaggct tgtggattct gcaaaccaac ttcaattggg
     3001 aagcctgaat cttgataatc caagaaaatt ggaggggaga tacctagagc tttgcaagta
     3061 gccgcaagtt catcaacacg taataaaccc cgttccggac cttttgggta actattgggc
     3121 tccatgtctg ggtggacaat ttcaccctct tctcctcgtg tggcacagat aaggctgacc
     3181 tgatggcctt catcagcata tctggcaaga gttcctccgc atgagaaaac ttcgtcatcg
     3241 ggatgtgcaa atatcgctag taatttcata tacaatcaac ctttcattga gaaaaaatct
     3301 gctttgccgt ttgttactgc ttcctcattt ggactttaga ccatttgaat agcaaggatt
     3361 ttagtattag agtaaggacc aatattacaa gatcaggaag aatttagcga cctcccattc
     3421 cagaaatagt aattccacga ataatccatt tttgtgcaaa aagatagatg attacaatcg
     3481 gtaataacgc aatgacagct gctgacataa gaagttccca ttgcacactg tactgtccct
     3541 gaaaagaact aagcccaact gggatcgtgc gcatttctac cctgttagtc accaccaaag
     3601 gccacaagaa ctcgttccaa ttgcccaaaa aagtaaaaat tcccaaagct gccgtcactg
     3661 gacctgcatt gggcataata actttccacc agatttgcca tgaggatgcc ccgtctatcc
     3721 gcgcagcatc atcaagcact gtcggtattc cccggaatgc ttgacggtag agaaaggttg
     3781 caactgctga aaatgctggt ggtagaatta gggcagcata agtgtcgtac caaccaaatt
     3841 ctcgcacgat caggaagttt ggaatgagaa gtacagggaa aggaatcatc aaggtggcaa
     3901 gataagtaaa aaatataatc tctcgcccgc gaaacttaag tcgtgcgaaa gcaaacgctg
     3961 ctaaagacga cgtgatcaaa actatgaaag tcaccgacaa cgctaccacg atactgttga
     4021 catagtatcg gccaaatggc accacagtaa gggcatccaa atagttttcc caacgaatct
     4081 cgcttgggat ccagataggt ggataggtaa atatttcctt tggatttttt agcgatgtgc
     4141 ttaacatcca aagaaaaggc agcagcataa ttaagccgac aagcatcaaa acaagatgta
     4201 aaaatcctct ccgaagcttt atgtacatta tttctttccc ttatgattat tacgagcttc
     4261 aaataagtta agattcatag tgtacccatc gtcgttgcaa ccaggtttga acaatcatca
     4321 gtatcatgat caccaagaag agcagccatg caatagcagc tgccttgccc atcaacccat
     4381 attcaaaagc attctgataa atgtactgaa ccacggatag tgatgattgc gctggccccc
     4441 cccctcgtga gaacataggg ctcagcaaac aattgaaatg ctccaatcat ttggataatg
     4501 atcaagaaaa aggtcgttgg acttaccatt ggaatggtga catgaaaaaa acgttgccaa
     4561 ttcccagctc catcaacacg agcagcatca tataacgact caggtacccc ttggaggcca
     4621 gccaagtaaa tcaccatcga aaaacccacg tttttccaaa tggttagcat ggcaaccgca
     4681 ctttttgata gttcttgact gtttaaccaa tcaggcggtg caaccgccca acctgtgact
     4741 tcagcaatct gccagatcca accacttgca attccaaaat ctctgtttaa aaaaaactga
     4801 aacacaattg caactgccac aatattagtg atgactggca taaagatcac taaccggtac
     4861 caaataattc cacgaataga ctgattcagg gccacagcta agatcagccc caacagtagc
     4921 tgcactggga caattaaaat tacgtaaatt atggtattac ctgctacctt caaaaaaatt
     4981 cggtcggcag ttaacaactc gtagtagttt tcaaaaccta caaattttat aggttgtatt
     5041 agattccact ctgtgaaact aagaactaac gatattaaga caggaatcaa tacgaacgca
     5101 gtaaatccaa gtaaacttgg gagcaagaaa agatacccct cgctctgttg gcgggaactc
     5161 cagcgccccc attgggaata aaagcgagtt gatttttcca tcggggaaat tcgtggggtg
     5221 agtgttcaag aatagatata catgaaccta ctttgataaa cttgaaaaca ttaaagagtt
     5281 ttcatagagt accacagcct gatagacaga gatatcatct cttcagagca acaaacaatt
     5341 gaagtcctgg acatcattct acctaaatga ttaagtgtgt ttgaattgtc ctactcaatt
     5401 gcaattgaac gtttcttcct ataggttgct tctcattggt cagtaacttt gtgaatgcat
     5461 ctcactcttc agtgtaacat acaaccgttc aagttcctta ctttcacaaa tctatcttta
     5521 agtaaacaat ctctattgtg ggttcctcgt tatagacgaa cccacaaaaa tcagtaggaa
     5581 ctagaagccc agtcccttgc tttggaggaa agagtttact tccttacaag tttgctcgag
     5641 tactgaacga acaggctcac tacctgacca gatccgacta agatttggtg agatcacttt
     5701 gccgttaaga gtgccccagt taggaaagta gcctgaacgt cccttttttg cattatttcc
     5761 ttcaattatg aatgcggcac gatttgctgg tggagcattg attccaagga aagcgtcaga
     5821 cactgcagtg gaacgaagcg ctggtaaaat ttcacctgaa gtagagtaaa ccatctggcc
     5881 cccctcggta ctctgcaacc atgagaggaa tgtccatgcg gtatccttgt catcactgta
     5941 cttgctcatt gtccaagctg caccaccggc cgtaccagaa cgctgcccac ctgcaggtat
     6001 aggtactgca gccacatcat aattcatttt ggcagcatta aaagctgaaa ttcgactggc
     6061 attctgaata atcatagctg cttgtccact ctgaaacaca ccagcatcac cgccagcctg
     6121 actaagtgca gagggtcgca tagccaattt ctcatccatc agccccgcaa aaaaactaat
     6181 cgcttcaact gcgactggtt ctgacaatgt acaggccgtt ggattgacca tatcatcaag
     6241 gatagtaccc cggttctgtc ccaggaacaa ctggtacttg cccccttccg ctcccagggc
     6301 atagcgctgt acccgaccac tggtagtgat ctttgaaagt tgcttcgccg cagctttcag
     6361 gtcatcccat gtccaaaaat tggtaggata ggatacacct gcctcgtcaa agatgtcctt
     6421 gttgtagtaa aggacttcaa tcccgatgtc gcgtggcaag ccataaatcg ttcccttcag
     6481 ttttccaaaa tctaaaagtg atggccagta atccgatgtg tcataaccgg aggccttaat
     6541 gaacggatca agcggctcaa gcacaccctg tcccgcatag gtcaccaccg gtgataggaa
     6601 cagcacatcg ggtatgcctt ccttgtcccc tgccgcccag agagtcttga gcttggtaaa
     6661 gtaatctccc caaggctgcc cccacaggat tatctgaata tcggggtgag acgccatgaa
     6721 ggcatccgca actctttgat gggtcttaat ttctgcaggg ctgccccaca ttccccaagt
     6781 caccgttgtt ttggcttggg caatgccgac acaaacaagt aggccgcagg caactcctat
     6841 cagcgttttc atcagactac ctgtaatttt catttgtatc tccttaacac gtccagcgac
     6901 agcggcagtt gtaataaatt tgcgccgact cgatatatta ttttgaactt cgattttttc
     6961 ttcactcata gcactctccg tcttaggtgt tggaggcaaa tcttttttca aagcatttgt
     7021 cctcaaaatt cctgcgtaat tattgacgcc gggttgttgg ggaaatcaca acttgatcat
     7081 agaaaatcag gtcatgtaaa agctaatgta aactaataca aattatttat caagaaaaaa
     7141 taaaattgtt taaaagtttc actttttctc actatgttat gacggcacgt ggaggtcctt
     7201 tttagtatca gtgagattta agaatgtgtt tcagattgag gaggttggtt gccggcatat
     7261 ctgacgtaaa cgaaactgga acaacccatt tcaacccacc aatacttgat atcacctttc
     7321 tcgcttataa gaaaatccgc atttgatggt tcaaatttaa aatataataa ataattcttg
     7381 catttataaa aaattgtgat accgtatgaa ctttctatta atattccatc atttcggtag
     7441 tggactggtt cctggtgaaa tgcacttagc tgatggacag gagccagtcg cagttggaag
     7501 ctgcgacaat ttgaccgata acgacaccat taccggaaca catcgtccac actattttgc
     7561 cattgccaag ggaattcccg atggccttgc tcgatgtgac ctcccaacag gtcatgcaag
     7621 tggtccacaa tctcaacagc agactcatga agtgccttgg attcaagact cccaatgagg
     7681 ccttccgcca gcttgccggc gttgatgctg aaaaaattct tggttatgca cttataacat
     7741 gaattcaccc tatcgaattt cgatacaacc gcttgtattt tcagacttga aaggtgttcc
     7801 ttgattcttg acagccagaa aaagttatac cggaccaaga tcgatcaatt tataaagcgt
     7861 ctggaagcac tcatcctgag tcagtacgag ttgctggagg cagagttcgc caacagcaag
     7921 gatccggttc cttttggaga gaggctccga ctacagtacc agcggattga agaaggtcag
     7981 gaatggggtc aggcctggga aagcgcctgg tttcacctgc aaggccggat tccagaaaaa
     8041 tggcaggagc aggaggtggt ggcccacctg gatttcaatg gcgaggccct tatcttttca
     8101 ccacaaggag atccacttca aggactgacc aacggcagtg tttttggcaa tgtcacccgc
     8161 cacaccttca ttctcacccc caaggcccag ggtggcgagc ctgtcgatct ctgggtcgaa
     8221 ggtgctgcga actccatcgt gggaatcaac caacccagtg atccacctcg cggcactgtt
     8281 gatcggcagg gtaactacct tggaaaggtc aatcggatgc gtatagcgcg cttccatcga
     8341 gaactctatg cactccggct ggatatggag ctgctgaatg gtttgctcga taccgttcct
     8401 gagttcagta cccgtcacgc tcgtatcctc aagagtctgg tggagtccac caacggcttc
     8461 cgggacgatc ccgatcgtgc cgggatttgc cgggagatcc tagcaccgct gcttgctcag
     8521 ccggctcact ccagctcacc cacaatgatt accgttgggc atgcccatat cgacaccggt
     8581 tttctttggc ctgtacgtga aaccatccgt aaagcagggc gtacctttgc caaccaactt
     8641 gatctgattg aacgctatcc ggattacgtc tttggggctt cacagcctca gcattatcag
     8701 tttgtaaagg accactatcc caacctctac gaacgcatca agaaagctgt ggctgaggga
     8761 cgctgggaac tccagggtgg catgtgggtg gagccggact gcaacctgat cagcggtgaa
     8821 tcaatggtgc gtcaggtgat gcatggcaag aacttcttca tggacgaatt cggagtggaa
     8881 gtaaccaact gctggattcc ggacgttttc gggtactctg cttccatgcc gcagatactt
     8941 aagagcgcgg gaatacatta cttgctgaca cagaagatga gctggagtca gttcaaccgg
     9001 ttcccctaca ccaccttccg ctggcgaggc attgaaggca cagaggtgct cactcatttt
     9061 cctccggaag acaactacaa cagtgaactc caaccctgcg gcctgcaaaa ggcggagcgg
     9121 cagttcgtcg agaaatcgat tctggatgaa atgctatgcc cgtttggtgt gggtgatggt
     9181 ggtggcggtc ccactgccga aatgattgaa cgtggacttc gccagcaaaa cctggaagga
     9241 gtgccaaaag tccaattcgg gcgggcggat cattgctttg cacggatcgc caagcaacag
     9301 gatcaactgc ccgtgtggtc gggagagctt tacctcgaga tgcatagagg aacctacacc
     9361 actcaggctc gaaccaagcg cgggaatcga ctgttggaac aaagccttcg ggaaaccgaa
     9421 tatctcttgg ccctgggtaa acttaaggat tatcccatcg agtggctgga cggcatctgg
     9481 aagaaactgc tgatcaatca atttcacgat atcctgccgg gttccagcat cagggaggta
     9541 tatgaggtga cggaacgtga gcatgcggaa gcactcgaag actgcgcccg gattcgggcc
     9601 gaggccgctg acaaaatcct tcagcatgat gcagaagggc tgaccgtcat caacaccctg
     9661 aatgttccct gcacccgtga aatccaactc cctgaagact ggcaaggcgg attagtggat
     9721 gattcgggca accccatcga catgcaacag gacagcgacg gcaccattac cgcacagctg
     9781 cagctggctc ctcaggccat ctgcaccctg catcgagaag gaagaatggc cctcgctcag
     9841 tcagtccagt cactggttat ggaaaatgat ctggtgcgct acgagttcaa cgagcacggt
     9901 caactgctga gtgcctttga taaggaaatg cagcttgaaa tgctggcaga aggggagccc
     9961 gggaacatct tgacgctcta tgaagatcgt cctcacgact gggatgcttg ggaagtggac
    10021 atcagctacg aggaaatggc gctggaaact gccaggggca agcgatggac cagcacaggc
    10081 cagggaatgg ttcgccaggg tctcaagttc gaactcagca taggccagtc aaccatcaag
    10141 caaaaaatca ccctcagtca aaaaagcaag gcactggagt ttgacacttt agtggagtgg
    10201 caggagtgcc atcgcatgct gcgcacggcc ttccccgtca acgtccatac ggattcggcc
    10261 acgtttgaca ttcagtatgg atacgtccgg cgccccactc accggaacac cagttgggat
    10321 atggcgcggt ttgaggttgc aggccaaaag tatgccgatc tctctaacca tgaacgaggg
    10381 gtagcgttga tcaacaactg caaatatgga cacaaggttc atgaaaacgt tcttgatctg
    10441 aacctgctcc gctcccccac ctatcctgat ccggatgctg atttgggtct ccatgaattc
    10501 agttactgtc tctttatcca tcagcacgaa ttggtgaaat caggtgtgat cgcagaggcc
    10561 ctgcaattca atcagcctcc cttgttgttc cccggcaagg cttcagtaga attgcagcca
    10621 cccgttcgag taaacggcaa aggcgttggc ttggaggtac tcaaacgtgc tgaaaaagaa
    10681 aactgctgga tctttcgact ggtcgaacgc tatggacaga caggcattgc ccaaattgaa
    10741 ctgctggaat ttgaagcacg ggttcaggaa accaatctgc tggaatggga agacatgggt
    10801 ggattactca catctccact cactctgaag tttaaaccct ttgaaattcg cacctttaaa
    10861 atacgatcag gactaaggca agggccatct tgaccgctgg agaacacaaa ggtgtgcaaa
    10921 tctctttctc ttcttgaacc taccctattc ttgtaaaaca aatcgaatga tgatatattt
    10981 tgtgtgctcc tgtctgtgtt tagtgcatgt caatttaagt ttgtgctctt gatgaggtca
    11041 ttttttcaat cctcagcgca taaagtcttc actttgaggg aattgtgtta tgtaaaaaat
    11101 gaaaaacact taacaacgtc taagaaggaa ggtagtagaa gataaaggag ctaaaatgtc
    11161 tgcacttaaa aatgtttcaa gttaaaaagt caagggtagg acgatgaaga gttacacaat
    11221 caccatcatg atgaatttgt attgaagccc aatttgcctg aaacaggaat tctgggagta
    11281 aaaaccgaca cttaacatgc aaggaaattc cttgattaaa gaagctcgta aaagagctct
    11341 ggatgtgttg cataactgca tcactccgca tgggtttcgc gcttcagctt tagctgcagg
    11401 atatccacag atatgggcac gagatagtat gatcgtatct ttgggagcga ccactactgg
    11461 tgatcctgat ttactggcag ccagccgcgc ttccttggag acaatgagta cccaccaatc
    11521 ttcacgtggc ctgatccatc ttaatgttaa tccggataca ggttacgtta gtacggaaaa
    11581 tgcaggtgga acagactcga atctatggta cattctcggt cattttcttt accagcaagc
    11641 ctctagtgac tccgattttt tgagatctta ctggcctcag attgaacggg ccatgatctg
    11701 gttaggctat caggatataa atgagtgtgg ccttttggaa gttcctgagg ccggaaactg
    11761 gatggatttg cttgccgtcc gctataacgt tctctacgat aacgtacttt actatgctgc
    11821 cactctagcc tacgaaaaaa tgtctgctca aatggctctt caagcagatg tgcaaaattt
    11881 gattgaccct gcaggcatcc atcaacgcat taacttatta ctgtggattg aaaggacttg
    11941 ggatgctgta cattttgctg agcatttaga gaaactaaag tccatgcact tagagtggtt
    12001 tatgctatac cacaacatgg gaacaatctc aagtcgccca tactatatgc catgggttgc
    12061 ctttcgcgag tacggcgatt ggtgtgacag cttcggaaat ctattggcaa tcttaacagg
    12121 agtcgctgac tataatcgat ctgaacaaat actgtgttac atgcgacagg ttggcatgac
    12181 tatcccttta cccaccaaag caattcaccc accaattttt cccggtgaca gtaactggcg
    12241 cgagtactat cgcagcagga atctaaacct tccggatcaa taccataacg gtggtatttg
    12301 gccaatgatt ggtggattcc atgttgcggc actggttagc cacggttgcc aaaaaaaggc
    12361 tgaatccttg ctgactttgc tggctgaggc aaacctaacg ggcatttctg atggcttaga
    12421 attcaacgaa tggatacacg gcaaaagcgg tcaacccatg ggatatgcac gacaggcttg
    12481 gtctgcgtct atgttccttt atgctgacca tgcggttcat tcagggaaat taccactctt
    12541 cgatgaactc cagtcagcaa aacccgaagt tgtacgtgcc agtgaagtta atgaagttaa
    12601 atattctggt gggggcggac cagtgtgaat caaataattc tgttgcgaat gtattaggga
    12661 ttttaaaatt gttttattgc tccataacat attgacaatt tagaggatta aaaggtgcga
    12721 tcacccttgt cttcaccttt cctcataatt taccattata tatccgataa tattaaagat
    12781 taatgaattg ccaatttcag taatgggacg aatcaaatgg gacaggtttg tccaatcaca
    12841 ttccctctcc aacctatata aatactcaga attagtatat ccatagagtc ccccctttaa
    12901 gtttccgcag aaatatcaag ccctgtctat ctattaaaat tatatacttt gtcaacttaa
    12961 tgaacataag gtggtaaaag ccgtatccgt tatagataaa aagatctcag attttgatgc
    13021 ttaattattc ggtggacttt tgaaaactct aattcattga acctatagat aaaatcaatt
    13081 ggagtatatt acaagaattg ggggaggttg tggatggcta cctgactgtg aagaattag
//
LOCUS       DTVD01000006            3458 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10455_length_3458_cov_220.943579, whole genome
            shotgun sequence.
ACCESSION   DTVD01000006 DTVD01000000
VERSION     DTVD01000006.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10455_length_3458_cov_220.943579"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="EYO68_00205"
     CDS             complement(<1..933)
                     /locus_tag="EYO68_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC06259.1"
                     /translation="MADQRIAKSSTMYLFERLVKNYPKRFGTFLALIGVLVLTPDTMV
                     MRFSNLDRWPLMGWRGVLMGVTLLIIWRFLFVSNTKKKWRTLYTWPGIIVIISFSINS
                     VTFTLGIQETSVMLVLTALATMPIFAAILSSFMLRETQGWLGWLTIMAAMVGVAIVVS
                     DGNNAISQPEGSPVLGAAYGAITALGLALAFTMARKYRDLEVVPAAAVGALISGIIGF
                     AFSTEGSIFNAPIWTVFTMGVVILPVSFAFLITAPRYTTSAIVSLIMLLEMVIGPFWV
                     WFGIGERPSMIMIFGGCIVIVVIAFHIIRTQFYSS"
     gene            complement(1398..1799)
                     /locus_tag="EYO68_00210"
     CDS             complement(1398..1799)
                     /locus_tag="EYO68_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HIC06260.1"
                     /translation="MNKLLIAFQMLLLVFLVGCTNEAEYVGELQNGKPHGKGSITYEN
                     EEIYDGEWKEGLKEGKGTLTSPDGGKYEGEWKNDKMEGQGTYTWPNGNKYEGEWKDGK
                     KHGDGTILFKDGSKMEGEFRDDSPWKTTLSD"
     gene            complement(1908..2528)
                     /locus_tag="EYO68_00215"
     CDS             complement(1908..2528)
                     /locus_tag="EYO68_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIC06261.1"
                     /translation="MKSSHATANDAKHAVPTTYYQFLEISVRELLIEKGVLSAEDIRN
                     AVKEMDSRGPELGAQVVARAWVDPQFKQSLLQDAPSAVKALGLDIGPLQLLVVENTPE
                     VHNLIVCTLCSCYPRSLLGLPPDWYKSREYRSRAVREPRSVLLEFGTDISDEVEVRVH
                     DSTADLRYLVLPQRPEGTGHLTEDELAALVKRDSMIGVTEVCFSAI"
     gene            complement(2525..2824)
                     /locus_tag="EYO68_00220"
     CDS             complement(2525..2824)
                     /locus_tag="EYO68_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIC06262.1"
                     /translation="MTPLFQTGNAVRVRKGSPPGHIRTPFYVRGCKGVIERVAGEYRN
                     PEELAFGRFDTPVVPLYRVRFQQQEVWPDYQGNPQDSLDVEIFEFWLEPANQEKT"
     gene            complement(2821..3156)
                     /locus_tag="EYO68_00225"
     CDS             complement(2821..3156)
                     /locus_tag="EYO68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HIC06263.1"
                     /translation="MERDFTSRSHHDMGGLEAGHIQYNEHDYEFWEKNVDAMLVLLTS
                     MSPKLMSVDQLRKGIESLPPDAYEKMSYYERWIFSITQALQYNGVLTTEELSRKLEEV
                     QSRYETEEV"
BASE COUNT         1008 a          757 c          674 g         1019 t
ORIGIN      
        1 gcttgagtag aattgtgtcc gaataatatg aaaagcgatg actacaatta ctatgcagcc
       61 accaaaaatc ataatcatac tcggtctttc accaatacca aaccagaccc aaaatggtcc
      121 aattaccatt tctaacagca taattaaact tacaatagca gatgttgtat aacgaggagc
      181 tgtaataaga aacgcaaatg acacaggaag tatcacaact cccatagtga atactgtcca
      241 gattggcgca ttaaaaatac tgccttctgt actaaaggca aaaccaatga taccactaat
      301 cagggctcca acagcagctg cagggaccac ctcaagatca cggtattttc gcgccatagt
      361 aaaggcgagt gccagtccaa gtgcagtaat agcaccataa gcagcaccca atacaggaga
      421 cccttcgggt tgactgatgg cgttgttgcc gtcactgaca acaatggcga caccaaccat
      481 tgcagccatt atagttaacc aaccgagcca tccttgtgtt tcgcgcagca taaaacttga
      541 tagaattgct gcaaatatcg gcattgttgc taaggcggtt aatactaaca ttacagaagt
      601 ttcctgaatt ccaagtgtaa aggtaacgga atttatggaa aaagaaatta tgacaattat
      661 tcctggccaa gtataaagcg tacgccattt cttttttgta ttagaaacaa acaaaaagcg
      721 ccagataatc aatagagtaa ctcccattaa tactccacgc cagcccatca gcggccatcg
      781 gtcaagattt gaaaatctca ttaccatggt gtcaggtgta agaaccagca caccaatcag
      841 ggcaagaaaa gtcccaaatc gtttcggata gtttttaact aatctttcaa ataaatacat
      901 tgttgagctt ttagctattc gttgatctgc cataaatcaa aggcagtatc accctcaatt
      961 attaattaat tcaaactcag tgaaaggaag tctgaaaact gtcaaagttg atggaggtaa
     1021 atggtggggg aaaaattatt aagaaatttg atattttttt gcattcgctt aaatattgat
     1081 attacaaagt aaatattgtg atgatgaatg gtcgttaata tttgtacttc gggtttttag
     1141 ttccaagcat gctataataa gcaagtaact ttactgcacg acttatctat aatattttaa
     1201 gccacttgag tttctcatga atcataagtt cccgtaaaaa aattatatta ttgactgtgg
     1261 attgtcattt cgaccgaagg agaaatcgta taaaatgctg ttattgtaca agacccctca
     1321 catatgtttg agaggacacg acaaaattac tgactttcga gttgggtaga gtagtgagtg
     1381 atgtaaataa aagtgtttta gtcagaaagc gtagttttcc agggactgtc atctctgaac
     1441 tcaccttcca ttttacttcc atctttaaac aatatagtac catctccatg cttttttcca
     1501 tccttccact ccccttcata cttattccca ttaggccagg tgtaagttcc ctgaccttcc
     1561 attttgtcat tcttccattc accttcatat ttacctccgt caggtgaagt gagagttcct
     1621 ttaccttctt tcagcccctc cttccattct ccgtcataaa tttcctcatt ttcataggta
     1681 atgcttcctt taccatgtgg tttcccgttt tgcagttctc ctacatattc ggcttcattt
     1741 gtacatccaa ccaaaaatac taataataac atctgaaatg caattagtaa tttgttcata
     1801 gcaccactct gatttaagtg ttttattgaa cctgcagata aagcatattt tgcaatttta
     1861 tgatcaataa ttattctact caggatgtac tgtacaagtg attcttatta aattgcacta
     1921 aaacaaactt ctgtgacgcc gatcattgag tcacgtttta cgagtgcggc aagttcatcc
     1981 tcggtgaggt gtccggtacc ttctggacgt tgcggcagca cgaggtagcg caaatcagct
     2041 gtgctgtcat gtacccggac ttctacttca tcagaaatat ctgtaccgaa ttccagcaac
     2101 accgatctcg gttcgcgtac cgcacgtgaa cggtattcac gacttttgta ccagtccgga
     2161 ggtagaccga gcagtgagcg cggatagcat gagcagaggg tacagacgat taaattgtgg
     2221 acttctggcg tgttctccac taccagcagc tgcaaagggc cgatgtctag tcctagtgcc
     2281 ttgacggcgg atggtgcatc ctgcaaaagc gattgcttga actgaggatc aacccaggca
     2341 cgagcaacaa cttgtgcacc aagttccgga cctcgggaat ccatttcctt gactgcattc
     2401 cggatatcct cagcactcag cactcccttt tcgatcaaca actcacggac tgatatttcc
     2461 aggaattggt aataagttgt gggcacggca tgcttggcat cgtttgcggt agcatgggag
     2521 cttttcatgt tttctcctga tttgcaggtt ctaaccagaa ttcgaaaatc tcgacatcaa
     2581 gagagtcttg tggattaccc tggtaatccg gccatacttc ctgttgttgg aaacgtaccc
     2641 gataaagggg tacaactggc gtatcaaatc gtccaaaggc cagttcctcc ggattacgat
     2701 actctccggc aactcgttca attactcctt tacagcctct cacataaaac ggggtgcgga
     2761 tatgtcctgg aggcgaacct ttgcggaccc gcacggcatt tccagtttga aagagagggg
     2821 tcatacctcc tccgtctcgt agcgggattg tacttcttcc aacttgcggc tgagctcttc
     2881 ggttgtcagc acgccattgt attgcagggc ctgggtgatg gaaaaaatcc aacgctcgta
     2941 gtagctcatc ttttcgtaag catccggcgg gagactttcg attcccttgc gcagttgatc
     3001 aacgctcatc agttttggtg acatggaagt cagaagtacc aacattgcat ctacgttttt
     3061 ttcccaaaac tcatagtcgt gttcgttgta ttgaatatgg cctgcttcca gtccacccat
     3121 gtcgtggtgg ctgcgactag tgaaatctcg ttccatgctc aactatttca aatgggttag
     3181 tgttgcaact ggacagattt tactactttt ttaaaaattg taaaaacttt atttgtcatg
     3241 tttaaggatt tgaggaaaat taatcataat tagactcttt ttaaatctga gggaatataa
     3301 agatcagata attcgaaatt catgtaataa tatttgcact atccaataaa aattatgtaa
     3361 cttttctaac aatagtacaa tattgctatt aagtattgca tgaaccaaat cggcacagtc
     3421 aatgagagaa ggtgctgcaa aacaatttca tttgtcct
//
LOCUS       DTVD01000007            3452 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10475_length_3452_cov_199.184280, whole genome
            shotgun sequence.
ACCESSION   DTVD01000007 DTVD01000000
VERSION     DTVD01000007.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10475_length_3452_cov_199.184280"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="EYO68_00230"
     CDS             <1..101
                     /locus_tag="EYO68_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC06264.1"
                     /translation="GEFRRDKEWNTLRYDKDGNIIEKIVRGKLKKD"
     gene            113..931
                     /locus_tag="EYO68_00235"
     CDS             113..931
                     /locus_tag="EYO68_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HIC06265.1"
                     /translation="MKLITTYRLQRFFGAGIIAGINIATFYYYNLPATGKNMKQDKLT
                     ESAIKEAKPGAKQFKLSDGGGLYLLVHSNGSKYWRFDFRFEGKQKSSSLGVWPEVSLE
                     EARSRRNEAKLKIREGINPIQEKKKKSAQQHEQVWKKENSEERSDPELQQVDQKPGQK
                     QTPQSTGKPTRDAVNLLKSNIYPELGEKPVSEINKQDMTAILKNVYGSRKEVIQIIWN
                     IYPNYPVVQLAVLFILLFVTIDFLPALVTTSLYFIITVCVTVGRDLWIENKQQY"
     gene            complement(1011..1319)
                     /gene="hspQ"
                     /locus_tag="EYO68_00240"
     CDS             complement(1011..1319)
                     /gene="hspQ"
                     /locus_tag="EYO68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HIC06266.1"
                     /translation="MDTFCKFSIGQIIHHLRFDYRGVIVDVDADFQGSEEWYREMAKS
                     KPPRDKPWYHVLVDQSNTTTYVAEQNLEEEPSPQPVLHPLVEQYFNRFEEGCYQTDFC
                     "
     gene            complement(1560..2084)
                     /locus_tag="EYO68_00245"
     CDS             complement(1560..2084)
                     /locus_tag="EYO68_00245"
                     /inference="COORDINATES: protein motif:HMM:PF01323.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06267.1"
                     /translation="MKKKYGLTVHWTHFPLHPDTPDSGLSLEDLFQGHGADIEAMKQR
                     MSALMTEENLAYGNRSHTYNSRFAQELAKWGESFPEGEALNLKLFEAYFAKGRNLAEP
                     VVLLEVAEAAGLDRDAAEEMIRKRSFRDAVDADWKRAHELGVTGVPTFVSGNRGLVGA
                     QPYEALEQLIRTSA"
     gene            complement(2240..3226)
                     /locus_tag="EYO68_00250"
     CDS             complement(2240..3226)
                     /locus_tag="EYO68_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIC06268.1"
                     /translation="MKTRKLGLDGPDVSAIGLGCMSFAGFFGTTDKAESFECLDAARD
                     QGITFLDTAELYGKGYSEELIGEYQQERGHRFSIATKGGIVVGGKRGENDNSESALRT
                     ALEGSLKRLKVEHVELYYIHRRDWSIEIEDVAETLTKFKNEGKIGGFGFSEIAPSSLR
                     RAHAVHPVTAVQNEYSLWTRYPELGMLQACAELGVAFVPFSPLARAMLTDTFPDPTGF
                     PDTDFRKNMPRFLEPNFSANCKMISGFRNFARSRGWSTTEAALAWILDQEEHLIPIPG
                     TRSAAHLKEWAGAAEIKLTDEDRTEIERILPVGFAHGDRYSDEQIVGIERYC"
     gene            complement(3267..>3452)
                     /locus_tag="EYO68_00255"
     CDS             complement(3267..>3452)
                     /locus_tag="EYO68_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIC06269.1"
                     /translation="LDSRKDVQNLDLMNLSGFCRNCLAKWYSAEAVERGEEITVDSAK
                     EIVYGMTYGEWKDRYQK"
BASE COUNT          941 a          827 c          775 g          909 t
ORIGIN      
        1 taggggagtt caggagagac aaagaatgga acacattgcg ctatgacaaa gacggaaata
       61 tcattgaaaa gattgtaaga ggaaaattga aaaaagactg atctgcggtg ttttgaaatt
      121 aattacaact taccgcttgc agaggttttt cggtgctggt ataattgctg gtataaatat
      181 tgctacattt tattattaca atttaccagc aacaggaaaa aacatgaaac aggacaaact
      241 aacagagtca gcgataaaag aggcgaaacc cggagcaaaa caattcaaac tgtcggacgg
      301 cggaggttta tatctgctgg ttcattcaaa tggctcaaaa tattggaggt ttgactttcg
      361 atttgaaggc aaacagaaat catcttcttt aggcgtctgg cccgaagtat cattggagga
      421 agccagatcc aggcggaatg aagccaaatt gaaaattagg gaaggcataa atcctattca
      481 ggaaaaaaag aagaaatcag cgcagcagca tgaacaggtc tggaaaaaag aaaattccga
      541 agagcgcagc gaccctgaat tacaacaagt agatcaaaaa ccagggcaga aacaaactcc
      601 tcaatcgaca ggaaaaccga cccgggatgc agtcaatttg cttaaatcta atatttatcc
      661 tgaacttgga gaaaagcctg tttcggagat caacaaacag gatatgacgg caatcctaaa
      721 gaatgtttac ggtagcagga aagaagtaat tcagattatc tggaatattt atcctaatta
      781 ccctgtcgta caacttgcgg ttctgtttat tttgttattt gtgacaattg atttcttgcc
      841 ggcactggtt acaacgagcc tgtatttcat cattacagtt tgtgttacag ttggcaggga
      901 cttatggatt gagaacaaac agcaatatta aacggctcat aactctcctt caaatcctcc
      961 ggaaaacacg taaatcaatg ttattgaatt gaatttccgt aaccacccgc tcagcagaaa
     1021 tcagtctggt agcagccttc ctcgaaacgg ttgaaatact gttctacaag agggtgcagg
     1081 acaggttggg gagaaggttc ttcttcaagg ttttgttctg ccacataagt tgtggtgttc
     1141 gactgatcga ccagcacatg ataccagggt ttgtcacgcg gaggtttgct ttttgccatt
     1201 tcacggtacc attcttctga tccctgaaag tcggcatcaa catcgacgat tacccccctg
     1261 taatcaaaac ggagatggtg gatgatttgt ccaatactga atttgcagaa agtgtccata
     1321 tgatttattg gggaaatata ttcaaatttc atagatcaaa aacttgtttc actgataagc
     1381 attgttttgt catcccggac ttgacccgga atccagcaca atcgtcacgt ttggaagcgg
     1441 aatgttaagc ctcgcccctg gattccagct tccgcgggaa tgacaacttt tagtggagga
     1501 attaagggta catgatactt aattcaaaaa aataacacac ttcatgtccc cataattctt
     1561 caagcgcttg tccggatcag ctgctccagc gcctcataag gctgtgctcc cacaagtccc
     1621 cgatttccgg aaacgaaagt cgggactccg gtgacaccaa gctcgtgagc acgtttccaa
     1681 tcggcgtcca ccgcatcccg gaaactgcgt ttacggatca tctcttcagc cgcatcacgg
     1741 tccagcccag cagcttcagc aacttccagc agtaccacgg gttcggcgag gttgcggccc
     1801 tttgcgaaat acgcctcaaa gagtttcagg ttcagcgcct ctccttccgg aaacgattct
     1861 ccccacttcg cgagttcctg cgcgaaccgg ctgttatagg tgtgggaacg gtttccgtaa
     1921 gctaagtttt cctctgtcat caaagcagac atgcgttgct tcattgcctc gatgtcagcg
     1981 ccgtgtccct gaaacaaatc ttccagcgaa agcccggaat ccggagtatc cggatgcagc
     2041 ggaaagtgtg tccagtgcac tgtcagtccg tatttctttt tcaatcgttc gacacgcccg
     2101 gtgacgaggt agcaccacgg tcagacgaaa tcgctgaata tttccagaac ttgagtggtt
     2161 tccattgaca taataattaa ggggacacga tacttaattc tgaaaaagga cacacttcat
     2221 gtccccgtaa ttcttcaaat cagcaatagc gttcgattcc cacaatctgc tcatccgagt
     2281 agcggtctcc atgcgcaaat ccgactggta aaatccgttc aatttctgtc cggtcttcat
     2341 cagtaagttt aatttcagct gctcccgccc actcttttag atgtgccgcg cttcgtgttc
     2401 ctggaatcgg tatcagatgc tcttcctggt ctagaatcca agccagtgcg gcttctgttg
     2461 tgctccatcc acgggatcgg gcaaagttcc ggaaaccgga aatcattttg cagttggctg
     2521 agaaatttgg ttccagaaaa cgaggcatgt ttttgcggaa atctgtatca gggaaaccgg
     2581 taggatccgg gaaagtatcc gttagcattg cccttgccag aggcgagaaa ggtacaaaag
     2641 caacacccag ttcagcgcag gcctgcagca ttccaagttc cggataacgg gtccagagcg
     2701 aatattcatt ctgcacggcc gttaccggat gtaccgcatg cgccctgcgc agtgaagacg
     2761 gcgcaatttc cgaaaatccg aagcccccga ttttaccctc gtttttgaat tttgtcagcg
     2821 tttccgcaac gtcttcaatc tcaatcgacc agtcccggcg gtgaatgtag taaagctcca
     2881 catgttcaac tttgaggcgt ttcagcgaac cttccagtgc cgtgcgcaat gcagattcag
     2941 aattgtcatt ttcgccccgc ttccctccga caacgattcc gcctttggtt gcaatactga
     3001 agcggtgtcc ccgttcctgc tggtattccc cgattaattc ttccgagtag ccctttccat
     3061 ataattcggc tgtgtccaga aaagttatgc cctggtcgcg tgcagcgtcg aggcattcga
     3121 agctttctgc tttatctgtg gttccgaaaa atcctgcaaa gctcatgcag ccgaggccaa
     3181 tggccgaaac gtccggtccg tcaaggccca gttttcgtgt tttcattatt tatccttcta
     3241 attttattga tcaaaaaggc tgttaattat ttctgatacc tatctttcca ctctccgtag
     3301 gtcataccat agacgatttc tttggcagaa tcaacagtga tctcctctcc gcgctccacc
     3361 gcctcggccg aataccactt ggccagacag ttgcggcaaa agccggaaag attcatcagg
     3421 tcaaggttct gtacatcctt gcgtgaatca ag
//
LOCUS       DTVD01000008           13034 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1055_length_13034_cov_206.715849, whole genome
            shotgun sequence.
ACCESSION   DTVD01000008 DTVD01000000
VERSION     DTVD01000008.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_13034_cov_206.715849"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            271..1344
                     /gene="mnmA"
                     /locus_tag="EYO68_00260"
     CDS             271..1344
                     /gene="mnmA"
                     /locus_tag="EYO68_00260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIC06270.1"
                     /translation="MARVAMAMSGGVDSSVAAVLLKEQGHEVIGLHMKLYHGPENETR
                     PKSCCSLDEAIDARAACHRLNIPFYVLDCQKEFRESVIDYFVEEYSRGNTPNPCVMCN
                     KEIKSNLLLKKVDELDCDYLATGHYAKIRFNPLIRRQQLIRPQDLRKDQTYFLHSIPA
                     DELHRLMFPLADIIKPEVRKIAGKLNLSAANKPDSQEICFVPKDYRNFLKQELNEVPE
                     HGEFISVSGEVLGEHLGLPFYTIGQRRGLGLSDSTPYYVVKIDKGKNRIVLGKEEDLY
                     SKTIFVSGVNWLSISPPKEKLHVTAKLRYSHQGVAASVFPESDNKVRLELDVPERAVT
                     PGQAAVFYQDEILLGGGWINDLP"
     gene            1369..1890
                     /locus_tag="EYO68_00265"
     CDS             1369..1890
                     /locus_tag="EYO68_00265"
                     /inference="COORDINATES: protein motif:HMM:PF01202.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIC06271.1"
                     /translation="MNIIITGFMGSGKTTIGRKLAKLIEYSFVDTDSEIEDDQGCSVS
                     EIFKYGGEECFREMETRLLEKLKNIDNSVIATGGGIVLREENRKMLKGIGKRVYLRVP
                     EEELARRLKNDRNRPLLMNNNPEIVVQGMLKERGLLYEQAECIIDTTQRSPHQIATEI
                     AKKLCNADHDRNF"
     gene            1865..2098
                     /gene="xseB"
                     /locus_tag="EYO68_00270"
     CDS             1865..2098
                     /gene="xseB"
                     /locus_tag="EYO68_00270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIC06272.1"
                     /translation="MQTTTATFESALKKLEETVDKLEEGSIPLDEALTTFESGVRWSR
                     ECKKFLENAEQRIETILKNENGEYEQKEFVLDK"
     gene            2371..4815
                     /locus_tag="EYO68_00275"
     CDS             2371..4815
                     /locus_tag="EYO68_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIC06273.1"
                     /translation="MTEVDQKILINVEDDETRIALVHGSTLNNLYIEQTHHTQKVGNI
                     YCGKVVKVQPSFQAAFIDYGEERHGFLSLSDINFQVYKPNQKGRGRPSITQILKPGQK
                     VLVQVVKDEIAHKGASLTTNISLAGRFLVFMPDSERGGVSRKIEDEEQRSRLRHLLKG
                     LGGEDSSAIIRTVGVDRSLTELKRDFMILRRTWNEIKDEYEEQSALGLLYQEEIAMVR
                     MIRDYYHENISEVVIDEPVAFQRALEFFQAHMPKDQKKLQLYLGEKSLFSSYDIEGHI
                     EVLHHKQVPLPSGGSLVIMPTEALVAIDVNSGRSTQERNIEATALRTNLEAAEEVAKQ
                     LRLRNLGGLIVVDFIDMDNSKNRLSVEKKIEEAMSADKAKTSFGEISKFGLLELSRQR
                     ISGSLARNSVVLTLVNRILRKIHDSAVEQKVLQVHIRLPLEIASHLLNVKRQRLTQME
                     MDYGIRISITPDTSLGSEEIPEMEVTLREQDGEETRTSVPISASDMRDEKPGGKKGRQ
                     KKEVAKKDSATAAPGSLEMKNKEKQSKQISNDTSKPSSKSKGKRPGTDTSLTQEHPDS
                     GSVVEKDNDKLTATEKKRTPEVEKKQKTVVKKQETKEKTDVGLFKSVHESLEVKDTPN
                     GKKSSQKRIVEENNDPEVSTFRSVHLDENLDSAKKTKRISHENPTKNIIGNPMFSSVH
                     ITDTIKSVPKVVFSKQDAKSRLTSGKTKSDSTKKSGKTAQGKPTAIKTAKKKQDSTKV
                     STRAKIKKTKVESNAVSVTKSKSSLKKVPVTKKETDNSTASRSFNSKNRSTSKSKQDT
                     KPKKNTETLPKKQKTE"
     gene            4879..6279
                     /gene="selA"
                     /locus_tag="EYO68_00280"
     CDS             4879..6279
                     /gene="selA"
                     /locus_tag="EYO68_00280"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:PRK04311.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIC06274.1"
                     /translation="MVKAMAYKNLPSITECDSFLRRHNLAYGKSVREIVNSVLNQVRQ
                     EIYQKKTGSVSYNNTQILERVAAKVRIQSTGAQRVINATGVVVHTNLGRAPLSAELLA
                     NALPRMSSYSTLEFDLSTGKRGSRDSKVRKLLRSLSGAEDAMVVNNNAAAVFLMLKAL
                     TTGLPEDKTPEVIVSRGELVEIGGSFRIPDIMREAGVKLVEVGTTNRCRLSDYEQALT
                     ENTAALLKVHPSNYEIKGFTESVSVGKMAGLAHSKGLFCFHDWGSGSFYRFKQRGLSE
                     YGTAEQELAAGPDLLAFSGDKLLGGIQAGVLLGKAENIQKLRKHALYRALRLDKVTLA
                     LLEETLEVYLDLKRLPELVPTVGMLELTVEEIQKQVKLFLERLKLPNKSALKCELRKT
                     ESRTGGGALPELPIESAALVLSHPEWSANDLQEWFRSQPVPIIVRVHEEQIWLDFRTI
                     LPHDSDELMSVITRLI"
     gene            6479..7762
                     /locus_tag="EYO68_00285"
     CDS             6479..7762
                     /locus_tag="EYO68_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06275.1"
                     /translation="MNSPTFLVKTLLIFLTCLFFTTPGWGQSNNSSGNIFGIQQTQLS
                     GFSDDERVKVLLLNLLPSGMTETAAEEIEKVLQLNVYNTNHFTVVGPSEWNAQIKDRD
                     PTLADCHDIACGVMIGKMFHADKVLVGTIHSEIMLNDNGKEEPSIILSIRLVDTRTNI
                     TDFTDEVQFNDLQMHDELFRMAERISENTLLLGNVLAIKHSGITINLGRAQGIKIGHR
                     LVISRRKSFKSDSVEKTAGKTFQKIALAEIVQVSDLSSEAVIVQKISSVAKGDQIQTY
                     VNNEKLIHLITQTRKELDIQKKLKPKNRVIRLGPTVTKGSTDFSNWSKRFNLTKSEHD
                     RWLYTTAGAGAATILFLSGTFNISGFLNILPWVAGAGSVYSGIKYFHYREMLNELSTE
                     GRSRGFLSGSLTNELPGWHWTPVKKGFNIAWVKHF"
     gene            8092..10401
                     /gene="ptsP"
                     /locus_tag="EYO68_00290"
     CDS             8092..10401
                     /gene="ptsP"
                     /locus_tag="EYO68_00290"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIC06276.1"
                     /translation="MAASETQEQLYFALQTFTEVNNIIASSHNSEETLARTVSMIAKR
                     IQVDACSIYIYDTDRGVLILKATHGLDPSTIESVHMPPSEGLVGLVLERSAPVQESQM
                     QEHPRFKAFPQTKEDSFSSFLGVPLIEHRKSFGVLVVHTTEPRIFSPEEVLILSSIAT
                     QISSLVSKALLLKQLDTATQEPTVAEKRKGTTLRLSGQAVAYGVAVGKAIRLQQSELE
                     EPQQSSTRSAENEMSDFQSAMDHTISDTMDLIEKVSDRVGTEEASIFHAHLMFLEDHH
                     FQSKIKTKIESGNTASWSIYQTVHEYLGAFEEIKDPYLSEKGSDLKDVGYRLLHYLGH
                     EVLSVTKKTGILIMRQLLPGDIARMDTTRIKGIILSTGGIVSHAAILARSLGIPAVCL
                     EDHELELIKDGDPLVIDGDSGLAVAYPEKEVLAEFKHLLVEQQHYFKHLEEFKDILCK
                     TSDGTRINLMANIALGSDQIQLPRYGAEGVGLFRTEIYFLSLDRYPDISEQTQVYREL
                     LDNVAEDKPVIFRTLDVGADKAAPYMGFQDEDNPFLGYRAVRRQLKQKSVLKEQLKAL
                     LLATGTRPNVRLLFPMITNVKEIRQAKELFEECRIEVEASGEAIAKIEVGMMFEVPSA
                     FLICDKFMSEIDFCSIGSNDLTQYVLAVDRNNPQIADLYDPLHPAVLQLIHRLVQTAE
                     EHQKSVELCGEMASDPDGCVILVGLGINSLSMNAPLIPVVKKRLSEISLPDAKILAQN
                     ALNATSPEEVRNLIRECFPDTHSKEKHKQ"
     gene            10398..11462
                     /gene="holA"
                     /locus_tag="EYO68_00295"
     CDS             10398..11462
                     /gene="holA"
                     /locus_tag="EYO68_00295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIC06277.1"
                     /translation="MMTQNDPHKICLIHGNQLLLVEETANTLIDQRLEGREQDWSLER
                     FNAQEMLKTIGESGKNSIDDLLISCETLPMLTDRKVIRIDNFELIKKAANKSDNSQAV
                     RLFEALENIIKNPPESLWFVFTSPAMREQDFSKPLYRSIKQGGGRIQKFVAYDNSTPY
                     NWVIQRGEQKGLPLTADVARLLINIVGNDLTDLDHELEKLSLYLSGKTVSEEMIKEHI
                     RGHKHFSVFRMTEALSRKELLPALEILDHQLQSAPREHVRLFALIIMQFRRLLIIHSM
                     FEQFYKESEIIGKISLPPFLSKQVLAQARNFTSEEVQNIYTELSKLDLRVKFKNTLAP
                     LLLQDLFQRICSGQFQNAVK"
     gene            11449..>13034
                     /locus_tag="EYO68_00300"
     CDS             11449..>13034
                     /locus_tag="EYO68_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC06278.1"
                     /translation="MQLNNDQPELNNENFPRLFSPIDLCGHSLRNRIVFGAHTANMSE
                     QGLPGERHLAYYRERALGGAAMLVIEPIPVHPAAVLTRGNFRNSTDEVIPHFRKITDA
                     VHEEGALILQQLYHVGQHGDSDNSYHANWSPSGMPSYHDSDGSHTMNETEIEETIEGF
                     VQAARRCSDAGFDGVEVWAAYHGMVDQFWTPWSNQRTDRWGGSLENRTRFSREIITRI
                     RKICGDDFIVGIAVNDEPDFEVALQRESIAEIIALHDRDHLLDYVTCGTGSYFDFYKL
                     MPTFLYPERLGAELAEVLKGAVTHALVIAESHIRTPENAEAVLSANQADLVSIVRGQI
                     ADPHLANKAREGRAEDIRTCLSCNQMCWGRRSRDYWISCLVNPSAGREFEWGGDRFQK
                     SKTPKRVLVVGGGPAGLEAARVSAERGHNVTLAEAGDRLGGQFRLAGMQPRRGQIIDL
                     LDWYARQLSQLGVEVRLNAFLDADEIKEFAAEKTVIATGSYPSDTGFQKAIPHVDKLP
                     GIELGGVFSAEDVMARRARPG"
BASE COUNT         4145 a         2577 c         2797 g         3515 t
ORIGIN      
        1 cttcaaaatt ttatttgaag gaacaaaaac agaaagatcg ggaaaagcac aaaagagaga
       61 tgcaggtcta taatgaaagg aataataaga aattcaatga atattttaaa cgcctacaac
      121 aaaaacgatc tcactctgca aactacagcg gtaggcattt cgattacaca agttacaacc
      181 aaaatattca agttgccgcc agtcgttaat ctcatttgag aaagccatct ttttttcttc
      241 caaatcatca ataaattcaa tacaataatt atggcacgtg tagcaatggc aatgagtggc
      301 ggcgttgact cgtctgttgc agcagttctg ctgaaggagc agggacatga agttatcggc
      361 ctgcacatga aactatatca tggaccggag aatgaaactc gtcctaaaag ttgctgttcg
      421 ctcgatgaag caatagatgc acgtgcagcc tgccatcgtc tgaatatccc attttatgtt
      481 cttgattgtc aaaaggaatt tcgagaatct gtaatagatt attttgttga agaatattct
      541 cgcggtaaca caccaaatcc gtgcgtaatg tgcaataaag aaataaaaag taatttatta
      601 ctaaaaaaag tagatgaact tgattgcgac tatttggcca ccggccatta tgccaaaatt
      661 cgcttcaatc ctctcattcg gcgccagcaa ttaatacgtc ctcaggattt gcgtaaagat
      721 caaacatatt ttctacatag cattccagca gatgagcttc accgtctgat gttccctctg
      781 gcagatatca ttaaacctga agtacggaaa attgcaggaa aactgaattt atctgcagca
      841 aataagccgg atagtcagga gatatgtttt gttccaaagg attatcgaaa tttcctaaag
      901 caggaactga atgaggtacc tgaacatgga gagttcatta gtgtatcagg ggaagttctg
      961 ggagaacacc tcggcttgcc attctataca attggacagc gccgtggact tggtctaagt
     1021 gattctacgc cgtattatgt tgttaaaatt gataaaggaa aaaacaggat agttcttgga
     1081 aaagaagaag atttatattc taagactata tttgtttccg gagtgaactg gttgtctata
     1141 tcacctccaa aagagaagtt acatgtaact gcgaagttgc gctactccca tcagggagta
     1201 gccgcttctg tttttccaga gtcagataac aaagtcaggt tggaacttga tgttccggaa
     1261 cgtgccgtta ctcccggaca ggctgcggtt ttttatcaag atgaaatact cttaggtggt
     1321 ggttggatta acgatttacc ttgagggttg tagattaaac attggattat gaatataatt
     1381 ataaccggtt ttatgggatc aggtaaaacc acaattggac gaaaattagc caaattgatt
     1441 gaatattcat ttgtagatac agattctgaa atagaagatg atcaaggttg cagtgttagt
     1501 gaaattttta agtacggagg tgaagagtgt tttcgtgaaa tggaaacacg acttttggaa
     1561 aagttgaaaa atattgataa ttccgtaata gcaactggcg gtggtatagt actgcgtgaa
     1621 gaaaatcgaa agatgctgaa gggaattggt aaacgtgttt atttgagggt gcctgaagaa
     1681 gaactggcgc ggcgtctaaa aaatgaccgg aaccgtccgc ttttgatgaa taataatcct
     1741 gaaattgtag ttcagggaat gttaaaagaa cgtggcttgc tctatgaaca agcagagtgt
     1801 ataattgata caactcaacg atctccacac cagattgcta cagaaatagc taaaaagctt
     1861 tgtaatgcag accacgaccg caacttttga atctgctttg aaaaaacttg aagagactgt
     1921 agataagctg gaagagggtt ctattcctct agatgaagct ttgactactt ttgaatcagg
     1981 tgtacgctgg agtcgagaat gcaaaaaatt tctcgaaaac gcagagcagc gaattgagac
     2041 aattttaaag aatgaaaatg gtgagtatga acaaaaggag tttgtactcg ataaatgaac
     2101 acggctttca acaaatgctt gaaagtcaaa actaatattt cagagatgtt ctgttccact
     2161 tgattgaaag aatgaatggg tggtgctgat ttctgattgc tgattgctga tttctagaaa
     2221 aactgaaaaa cgacgccggt aaaaacgaat gctgagttgt atcttaaagt ttgtttgcgt
     2281 cactgcagtt cctagattcg atttctaatc tgaaaatcaa atataattgc agaacatcct
     2341 gattttaaac ttaataaaat caggtaaata atgacagaag tagatcaaaa gattcttata
     2401 aatgtagagg atgatgaaac acgaattgca ctggtgcatg gttcaacact caataatctc
     2461 tacattgaac agacccacca cacccaaaaa gtcggaaata tctattgtgg aaaagttgta
     2521 aaagtacagc cctcatttca agcagccttc attgattacg gagaggagcg ccatgggttc
     2581 ctttctttat ccgatattaa tttccaagtc tataagccaa atcagaaagg tcgtggacga
     2641 ccttccatta ctcaaatcct gaagccggga caaaaagttc ttgtccaagt cgtcaaagac
     2701 gaaatagccc ataaaggggc atctcttact acaaatatat ctttagccgg acgcttcctt
     2761 gtttttatgc ctgatagtga gcgcggcgga gtctcccgaa aaattgaaga tgaggaacaa
     2821 cgttcccgct tacgacattt actgaaagga cttggtggag aggattcatc agccataatc
     2881 cgtacggtag gagttgaccg ttctcttact gaattaaaac gtgactttat gattttgagg
     2941 cggacctgga atgagataaa agacgagtat gaagagcagt ctgccctcgg actcctttat
     3001 caggaagaaa ttgcgatggt ccgtatgatc agagattact accatgaaaa tatctcagaa
     3061 gtagtaattg atgaacccgt agcttttcag cgtgccttag aattttttca agcacatatg
     3121 cctaaagatc aaaagaagct tcaactttac ctgggcgaaa agtctctttt ttcttcatat
     3181 gatattgaag gtcatattga ggttttacac cataagcaag ttccgttgcc ttccggagga
     3241 tcactggtca taatgcctac agaagctcta gttgcgattg acgttaattc aggcagatcg
     3301 acacaagaga gaaacattga ggctaccgca ttaagaacta atttagaggc tgctgaagaa
     3361 gttgccaagc agttgcgttt aagaaactta ggtgggttga tcgtcgtgga ttttattgac
     3421 atggataatt caaaaaaccg cctctctgta gaaaaaaaga ttgaagaagc aatgtctgct
     3481 gataaagcaa agacttcatt tggagaaatc tcaaaattcg gcttactgga attgagtcga
     3541 cagcgcattt ccggaagttt ggcgcgaaac tctgttgtcc taactttggt aaacagaata
     3601 ttgcgaaaaa ttcacgactc tgcagttgag cagaaggtcc tgcaggttca tatacgtcta
     3661 cctttggaaa tagcttcaca tttgctcaac gtgaagcgtc agcgtttaac acaaatggaa
     3721 atggattatg gaatacggat ttccataaca ccggacacgt cacttggttc tgaagaaatt
     3781 ccagaaatgg aagttacttt gagagaacaa gacggcgaag aaactcgaac ctcagtacca
     3841 atttctgctt cagatatgcg tgacgagaaa cctggtggaa aaaaaggaag gcaaaaaaag
     3901 gaagtagcta aaaaagacag tgcgactgca gcgccgggta gtctggaaat gaaaaataaa
     3961 gaaaaacaaa gtaagcaaat tagcaacgac acttcaaaac catcatccaa atctaaaggc
     4021 aaaaggcccg gaacagatac cagtttaacg caggaacatc cggattccgg atctgtagtt
     4081 gaaaaggata atgataagtt aactgcaact gagaaaaaga ggactcctga agttgagaaa
     4141 aaacaaaaga cagttgtcaa aaaacaagag acaaaagaaa aaacagatgt tggtcttttt
     4201 aagagtgtac atgaatcttt ggaagttaaa gatactccta atggcaaaaa atccagtcag
     4261 aagcggattg ttgaggaaaa taatgatccg gaagtatcaa catttagaag tgtacatctg
     4321 gacgaaaatt tggactctgc taaaaaaact aaaaggatat ctcatgaaaa cccaacaaaa
     4381 aatattattg gaaatccaat gttcagcagc gttcatataa ctgatactat aaaatcggta
     4441 ccgaaggttg ttttttcaaa gcaagacgcg aaatccaggt tgacatctgg aaaaactaaa
     4501 tccgattcga caaaaaagtc tggaaaaact gctcaaggca aaccaacggc aattaaaact
     4561 gctaaaaaga aacaggattc cacaaaagtg agcactagag ctaaaattaa aaaaactaag
     4621 gtggaatcta atgcagtgtc cgtaactaag tcaaaatcat cactaaaaaa agttccagtt
     4681 acaaaaaaag agaccgataa ttcaacagca tccagatcct ttaacagtaa aaacaggtct
     4741 acgtctaaat caaaacaaga cacaaaacca aagaaaaaca ctgaaactct tccaaaaaaa
     4801 cagaagacag aataaacttc tgaagaaagc ctctaatcaa aaatttcaac ttcataaaac
     4861 tgttgataaa tgtgagtcat ggttaaagca atggcttata aaaaccttcc ttctatcaca
     4921 gagtgtgatt ccttcctcag gagacacaat ctcgcttatg ggaaatcagt cagagaaatc
     4981 gtaaatagtg tccttaatca agtacgacag gaaatttacc aaaaaaagac cggatctgtt
     5041 tcatacaata acacccaaat tttagaacgt gttgcagcaa aagtccgaat acagtccacc
     5101 ggggcgcaac gggttatcaa tgcaaccgga gttgttgttc atactaattt gggacgagct
     5161 cctttgtcag cagaattgtt agcgaatgcc ttacctcgaa tgagttctta ttccaccttg
     5221 gaatttgatc ttagcaccgg gaaacgtggt tcacgcgact caaaagtccg caagctgtta
     5281 cgttcacttt ccggtgctga agatgcaatg gtggtcaata acaatgctgc tgccgttttt
     5341 ttaatgctca aggctttgac aacaggtcta ccggaagaca aaacaccaga ggtgattgtc
     5401 tcaaggggcg agttggtgga gatcggtggt tcttttcgaa ttcctgatat catgcgggaa
     5461 gcaggcgtga agttggtcga agttggtact actaatcgct gccgcctttc agattatgag
     5521 caagctctga ccgaaaacac agcagctctg ttaaaagtac atccatccaa ttacgaaatt
     5581 aaaggattta cagagtccgt ttcagttgga aaaatggcag gacttgccca ctccaagggc
     5641 cttttctgtt ttcatgattg gggtagcgga agcttttatc ggttcaagca acgtgggtta
     5701 agtgaatatg gaactgcaga gcaggagctt gcagcaggcc cggatttgct ggcttttagt
     5761 ggtgacaaac ttcttggtgg aatccaggct ggagtgctgc taggtaaagc tgaaaacatc
     5821 cagaaactga gaaaacatgc tctttaccgg gcattacgtt tagacaaggt aacacttgct
     5881 ttgttggaag aaacacttga agtatacctg gatttaaaaa gacttccgga actggttcca
     5941 acagttggca tgctagaatt aactgttgaa gaaattcaaa aacaagtaaa attatttttg
     6001 gagagactca aacttccaaa caaatctgcg ttgaaatgtg agctccgcaa gacagaatct
     6061 cggactggag gtggtgcttt accggaactt cctatagaat cagctgcact tgtattgtca
     6121 catccggaat ggtcagcaaa tgatttacag gaatggtttc gatcacaacc tgtaccaatc
     6181 attgtccgtg ttcatgaaga gcaaatttgg ctggatttcc gaacaattct tccacatgac
     6241 tctgatgagc taatgtcggt aatcactcga cttatttaaa cctgacataa ctggtttatc
     6301 agaagcataa cctaaatctc tgctgccaaa ttcaggtcta tccattctcc ttttacccaa
     6361 ttatctaaaa ggtctttttc ttgcttgtta atcggagcag cataatcttc atttaaagtt
     6421 gaatcaggag ttttcggcag tgtcttttta tatcagacaa ttaaaaatcc tacataacat
     6481 gaattccccc acatttttgg tgaaaacact tctgatattt ctcacttgtc ttttctttac
     6541 tactccgggt tgggggcagt ctaacaattc aagtggaaac atctttggta tacaacagac
     6601 tcaactaagc ggttttagtg atgatgagcg tgtaaaagta ttattactaa acctcctacc
     6661 ttcaggaatg actgagactg cagcagaaga aattgaaaag gttcttcagc tgaatgttta
     6721 caacactaat cattttacag ttgtaggacc ttcagagtgg aatgcacaga tcaaagaccg
     6781 ggaccctaca ctggctgact gtcatgacat tgcttgcgga gttatgattg gcaaaatgtt
     6841 tcatgcagac aaagtattgg ttggtacaat tcattcagaa ataatgctga atgataatgg
     6901 caaagaagag ccgagtatta tcctgtcaat tagactggta gatacacgaa caaacatcac
     6961 agattttact gatgaggtac aattcaacga tttgcaaatg cacgatgagt tgtttcgaat
     7021 ggcagaaagg atttcagaaa acacgttgtt acttggaaat gtgctggcaa taaaacactc
     7081 cggaattacc ataaatttag gacgtgcaca aggtatcaaa atcgggcacc ggttagtcat
     7141 atcccggcga aaatcattta agtcagattc agtagaaaaa acagccggaa aaacttttca
     7201 aaaaatcgca ttagctgaaa tagttcaagt tagcgatttg tcttctgaag cagttattgt
     7261 tcaaaaaatt tcatctgttg ctaagggaga ccaaattcag acttatgtca ataatgagaa
     7321 actaattcac ttgatcacgc aaacacgtaa agaactagat attcaaaaaa aacttaaacc
     7381 aaaaaaccga gttatccgct tagggccaac tgttacaaag ggaagtactg atttttcaaa
     7441 ctggtctaag cgctttaatc tgacaaaatc ggagcacgac cgctggctct acaccactgc
     7501 aggagctgga gctgcgacaa tactatttct cagcggcacc ttcaatatca gcggttttct
     7561 taacatctta ccttgggttg ccggtgccgg aagtgtttat agcggaatta aatatttcca
     7621 ttatcgtgaa atgcttaatg agctttctac agaaggacgt tcccgtggtt tcctttccgg
     7681 ttcgctcacc aatgaattac cgggttggca ttggactcct gttaaaaaag gcttcaatat
     7741 agcttgggta aagcatttct aaaatctgac agcttgattt ttcttttctc tctacaataa
     7801 attcctctcc tgtcaagttt ccaattttcc ggaatcaatt taaccggaca attgaactca
     7861 caatgtaaag aacataaatt acatcttgag tttaagacta aaaacttgga aactgtgaaa
     7921 catcttttag tggcaaactg cttaaagtta ctgcctaatt atctaaaata atccttcaaa
     7981 gttaaaaatc taaaatcatc tattcattcc aaataatgat aatgggaagc acatttatta
     8041 actgcttgat aaaactgacg agcaaaagtt cgcttgatcc gaaaggtgta aatggctgca
     8101 tctgaaacac aagaacagct gtattttgct cttcaaactt ttactgaagt caacaacatc
     8161 attgcctctt cacacaattc tgaagagact ctggccagga ctgtcagcat gatagcaaag
     8221 cgaatacaag tcgatgcatg ttcaatttat atttatgata cagaccgtgg agtactgatc
     8281 ctgaaagcaa ctcacggtct tgatccttcc accattgagt ctgttcacat gcctccttct
     8341 gagggactgg ttggattggt actggagcgc tctgctccag tgcaggaatc tcaaatgcag
     8401 gaacatccaa gatttaaagc atttcctcaa acaaaagaag attcattttc ttcttttctg
     8461 ggagttcctc tgatagaaca tcgaaaatct tttggagtac ttgtagttca cactacagaa
     8521 ccacgcatat tttctcccga ggaagtgcta atactgagtt caatcgcaac tcaaatctcc
     8581 agccttgttt ctaaagccct ccttctaaag caacttgata cagcaacaca ggaaccaact
     8641 gttgctgaga aaagaaaagg aactactctt cgcctgtccg gacaagcggt tgcttatggt
     8701 gttgcagttg gaaaagcaat acgcctgcag caatctgaac ttgaagaacc acagcagagc
     8761 agtaccagat ctgcagaaaa tgaaatgtct gactttcaat ctgcaatgga tcacacaatt
     8821 tctgatacta tggacttgat tgaaaaagtc tcggatcgtg ttggtactga agaagcttct
     8881 atttttcacg cacatctgat gtttttggag gatcatcatt ttcagagcaa aattaagaca
     8941 aaaatagaat ctggaaatac tgcatcatgg tcaatttacc agacagttca cgaatatctg
     9001 ggtgcatttg aagaaattaa agatccttac ctcagtgaaa aaggctcgga tttaaaagat
     9061 gttggctacc gtctcttaca ctatctaggg catgaggtcc tttctgttac caaaaagaca
     9121 gggattctta ttatgcgcca actccttccg ggagatatcg ccaggatgga cacaacacgg
     9181 attaaaggaa ttattttgtc aactggaggt atagtttcac acgcagccat tctggctcgt
     9241 tctttgggga ttcctgcagt ttgtcttgaa gaccatgaat tggagttgat taaagatgga
     9301 gacccgctgg ttattgacgg agattccggg ttagcagtag cttatccgga aaaagaagta
     9361 cttgctgaat ttaaacattt gctggttgaa caacagcatt attttaagca ccttgaagag
     9421 tttaaggata tcctatgcaa aacgagtgat ggtacacgaa taaatttaat ggccaatatt
     9481 gccctgggaa gtgatcaaat tcaattaccc cgctatggtg ctgaaggggt tgggctgttc
     9541 agaaccgaaa tttattttct ttcacttgat cgctaccctg acatttctga gcaaacgcag
     9601 gtatatcgtg aattgcttga taatgttgca gaagataagc ccgtgatttt ccggactttg
     9661 gacgttggtg cagacaaagc agcaccatac atgggttttc aagatgagga taatcccttt
     9721 cttggttatc gtgcagtacg ccgtcagttg aaacaaaaat ctgtactaaa ggagcaattg
     9781 aaggctttac ttttggcaac aggaactcgt cctaacgttc gcttactgtt cccaatgatt
     9841 acaaatgtta aagaaatcag gcaggcaaag gaattattcg aagagtgccg tattgaagtt
     9901 gaagcatccg gtgaagctat agcaaaaata gaagtgggta tgatgtttga ggttccaagt
     9961 gcctttttga tttgtgataa atttatgtcg gaaattgact tttgttcgat tggcagcaac
    10021 gacctcactc aatacgtact ggctgttgat cgtaataatc cccaaattgc tgacctttac
    10081 gaccctctgc atcctgctgt tctgcaatta attcatcgac ttgtgcaaac ggcagaagaa
    10141 catcaaaaat ctgttgaatt gtgtggtgaa atggcttcag atcctgacgg atgtgtgatt
    10201 ctagtcggtc ttggaatcaa ttcactcagc atgaatgcgc ctttaattcc agttgtaaaa
    10261 aaacgtctct cagaaatatc actgcctgat gcaaaaattc tggctcaaaa tgccttgaat
    10321 gcaacctctc cggaggaagt gcgtaacttg attcgtgaat gttttccaga tactcattcc
    10381 aaagaaaaac ataaacaatg atgactcaaa acgatcctca taaaatctgt ttgattcatg
    10441 gtaaccaact gttattggta gaggaaaccg cgaatacttt aatagatcag agacttgagg
    10501 gccgggaaca ggattggagt ttggagcgct tcaatgcgca ggaaatgttg aaaacgattg
    10561 gagaatctgg aaaaaacagt atcgatgacc ttttgatcag ttgtgaaaca ttaccgatgc
    10621 taactgaccg caaagtaatc cggatcgata attttgaact gatcaagaaa gcagcaaata
    10681 aaagtgacaa ttcccaagcg gtcaggcttt ttgaagcact tgaaaacatc ataaaaaatc
    10741 ctccggaaag cttgtggttt gtctttacca gtccggcaat gcgtgaacag gatttcagta
    10801 aaccactcta tcgcagcatc aaacagggcg gtggtcggat acaaaaattt gtagcctacg
    10861 ataatagtac tccatacaac tgggttattc agcgaggaga gcaaaaagga ttgcccctta
    10921 cagcggatgt tgcacgttta ctgataaaca ttgtgggcaa cgatttgact gatcttgacc
    10981 acgaactgga aaaattaagc ctctatttat caggtaaaac cgtctcagaa gaaatgatca
    11041 aagaacacat tcgcggtcac aaacattttt ctgttttccg gatgactgaa gctttgtccc
    11101 ggaaggaatt actgcctgcc ctggaaattc ttgatcatca gctacaatcg gctccccgtg
    11161 aacacgtgcg ccttttcgca ctcatcatta tgcagtttcg ccgccttctc atcattcaca
    11221 gcatgtttga acaattttat aaggaatcag aaattattgg aaaaatatct cttccaccat
    11281 ttctcagcaa acaagttttg gcacaagctc gaaattttac aagtgaagaa gtacaaaata
    11341 tctacacaga actttctaaa ctggacttgc gggtaaagtt caaaaataca cttgcacctc
    11401 ttctacttca ggaccttttt cagcgcattt gttccggaca gttccaaaat gcagttaaat
    11461 aacgatcaac ctgagttgaa caacgaaaac tttccacgtc ttttttctcc aattgatctg
    11521 tgcggacatt ccctgcgtaa ccgcatagtt tttggtgcgc acaccgccaa tatgtctgaa
    11581 cagggccttc cgggagaaag gcatcttgcc tattaccgtg aacgtgcgct gggaggtgca
    11641 gcgatgctgg tcatcgaacc gataccggtg catcctgcag cagttttgac acggggtaat
    11701 tttcggaaca gcactgatga agtcattcct cactttagaa aaatcacgga tgcagttcat
    11761 gaagaaggag ccttgattct gcagcagctt taccatgtag gccagcatgg ggattccgac
    11821 aattcatatc acgccaactg gtctccttcc ggaatgccga gttaccatga ttccgacgga
    11881 agtcacacta tgaatgaaac agaaatagag gagactattg agggcttcgt gcaggccgcc
    11941 agaagatgca gtgacgccgg ttttgacggt gtagaagtct gggctgctta tcacggcatg
    12001 gttgatcaat tttggacacc atggtcaaat cagcgcacag accgttgggg tggatcgctg
    12061 gaaaaccgta cacgcttttc acgggaaatc atcacacgaa tccgtaaaat atgcggtgat
    12121 gattttattg tcggcattgc agtcaacgac gaaccggatt tcgaagtcgc cctccagcgt
    12181 gaatcaattg cggaaatcat agcccttcac gatcgcgatc atctgcttga ttacgtcacc
    12241 tgcggtaccg gaagctactt cgatttctac aagctgatgc ctacatttct ctatccggaa
    12301 cgattgggag cggaacttgc ggaagtgctc aaaggtgctg taacccatgc cttggtaatt
    12361 gcggaaagcc atatccgcac accggagaat gccgaggccg tgttatctgc caaccaggct
    12421 gatctggttt ccatcgtccg cggacaaatc gcggatccgc atttggccaa caaagcccgg
    12481 gaaggacgag ccgaagacat tcgtacctgc ctctcctgca atcaaatgtg ttggggcaga
    12541 cgcagtcgtg attactggat ttcctgtctc gtcaatccgt ctgccggccg tgaatttgaa
    12601 tggggtggag atcgctttca aaaatcaaaa acacctaaac gtgttcttgt agttggagga
    12661 ggacctgcag gcttggaagc agcaagagta tccgcggaac gcggtcataa tgtcactctc
    12721 gcagaggccg gtgaccgcct cggcggccaa ttccgactgg cgggaatgca gcctcggcgt
    12781 ggacaaatta ttgatctgct ggactggtat gcccgacagt tgtcacaact tggcgtcgag
    12841 gtacgactca atgcttttct ggatgcggat gaaatcaagg aattcgccgc agagaaaact
    12901 gtaatagcta caggctcata cccgtcagat accggatttc agaaagcaat accgcatgtt
    12961 gataaactgc cgggaattga actcggtggc gttttttcag cagaagatgt gatggcacgt
    13021 cgggcacgtc ctgg
//
LOCUS       DTVD01000009            3400 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10746_length_3400_cov_190.414350, whole genome
            shotgun sequence.
ACCESSION   DTVD01000009 DTVD01000000
VERSION     DTVD01000009.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10746_length_3400_cov_190.414350"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="EYO68_00305"
     CDS             complement(<1..184)
                     /locus_tag="EYO68_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC06279.1"
                     /translation="MDIELSHEQRMLLETVQGFLEQEIYPHEEEVDRLGRVPLELGRQ
                     IEKRAIDIGLFAANLPE"
     gene            complement(210..1031)
                     /locus_tag="EYO68_00310"
     CDS             complement(210..1031)
                     /locus_tag="EYO68_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009831342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06280.1"
                     /translation="MTKENSLGWNFLRGFAWAGLAFLVLPTLIAVPVSLTPKRYLSMP
                     TGEVSLRHYINLFSSTEWMSSIGQSTIIAGFSALIATVLGTLCAIGLWRISSRASEVV
                     RGILLLPLIIPPIISAMAFYRIWVKLGLLDSYSGMIIAHAILAAPMVLITVSASLANF
                     DLRLEQASCNLGASIGTTLRRVVLPSIKPGIWAGAIFAFILSWDEIVVALFIAKFRIM
                     TLPRRMWDGIRENTDPTVAASAVVLVLVTISALTIYTIMTNHVRIQGSSEREENS"
     gene            complement(1028..1891)
                     /locus_tag="EYO68_00315"
     CDS             complement(1028..1891)
                     /locus_tag="EYO68_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06281.1"
                     /translation="MGPIIFNWKTSNASNAYWLLVVPLFGLIIALYAWPVINILWLGV
                     SDPELGIQNYKLLFTKSSLVKILWTTIRVCLITTLCSVVIGYLIAYAMVHLVGRERNW
                     MLTLVLISFWVSVLVRAFSWLTLLGRNGLVNAALVGSGLAEEPISMIRNEFGVLIGMV
                     HYMVPYAVLPLLANMQGIDHRVMDASRSLGAGPLLTFVRIYLPLTKPGVIAASLLVFI
                     ISLGFYVTPALLGGGRVLMVAEYVSVQILITVQWGVAAMLATLLLSGVLALLYIMSRF
                     MNIGEVFGARA"
     gene            complement(1912..3021)
                     /locus_tag="EYO68_00320"
     CDS             complement(1912..3021)
                     /locus_tag="EYO68_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC06282.1"
                     /translation="MQDKMFYKDLAFTGAKDFKSGKLGRREFLYLCALAGVASSSVIA
                     GDAEAAANEIVLWNWGGDAVKCHTSAFGAAFTKESGLPVKYDTSGPLQGKIKEMVNSG
                     NVTADVADADAFDAISLGKSGHLEKIDYSVVDKKKVLEGFAWDYGVSIIFYGYAFMYD
                     TKKFKGNPPSTWADFFNTKKYPGKRSLYKWANGALEGALMADGVSKDKVYPMDLDRAI
                     NKIKSIKSDTIFWGSGSEAQQMIVNGEVSMGMVWLNRAKAVEADTNGRYRLNMNEAIA
                     MPGAYLVPKGNPAGRANVMKFIASCQVAERQIEILRCHGMTPSNPEAFGMIPDDLKRF
                     AVTSEENLSRVLLNDPIWWAENGGNAVNRFLEAIG"
BASE COUNT          958 a          845 c          718 g          879 t
ORIGIN      
        1 attccggcag atttgcggca aataatccga tatcgatcgc gcgtttctca atctggcgac
       61 caagttctaa cggtactcga cccagacgat caacctcctc ttcgtggggg taaatttctt
      121 gctccaaaaa tccttgtact gtctccaaca acatccgttg ttcgtggcta agctcgatat
      181 ccatttccgt ctaatatttt aaaatatgtt taactattct cttcccgctc agaacttccc
      241 tgtatcctaa catgattagt catgatggtg taaattgtca gtgcgctaat agtgacaaga
      301 accaaaacga cagcggaagc ggccacagtc ggatcagtat tttcacgaat gccgtcccac
      361 attcttcgag gaagagtcat aatccggaac ttggcaataa acaaggcaac gacaatttca
      421 tcccacgaaa gaataaaggc gaaaattgcg ccggcccaaa ttcccggttt aatcgacggc
      481 agcactaccc gccgcaacgt tgtaccaatt gacgcaccaa gattgcacga agcctgttcg
      541 agtctcaggt cgaaattagc gagcgaggca gaaactgtga tcaacaccat tggtgctgct
      601 agaatcgcat gggcaatgat catacctgag taactgtcca gcagtcctag ttttacccaa
      661 attcgataga aggccatcgc cgaaatgatc ggcggaatga tcaagggcag caacagaatc
      721 ccccggacaa cttccgatgc cctagatgat attcgccaca ggccgatggc gcagagcgtc
      781 ccaagtacag tagcaattag tgctgaaaaa ccagctataa tcgtactctg tccaatgctc
      841 gacatccatt ccgtactact aaagagattg atgtagtggc gcagggaaac ctctcctgtc
      901 ggcatagaaa gataacgctt tggtgtcagt gagactggca cggcgatcag cgttggcagg
      961 acgaggaaag caagtccggc ccaagcaaaa ccacgaagaa aattccaacc taaactgttc
     1021 tcttttgtca tgctctagcc ccgaaaactt caccaatgtt cataaatcgg gacatgatgt
     1081 ataaaagcgc aagaactcca gaaagtaaca gagttgctaa cattgccgcc actccccatt
     1141 gcactgtgat gagaatctgc acactcacat attccgcaac catgagcacc cgtcctccgc
     1201 ccagcaaggc tggggtaaca taaaaaccca agctgataat gaaaacgagc agtgatgctg
     1261 caattactcc cggtttcgtc aatggcaggt aaattctcac gaatgttagt aaggggccag
     1321 caccaagact gcgcgacgca tccataaccc gatgatcaat tccttgcata ttggcgagca
     1381 gagggagaac cgcatacgga accatgtagt ggaccatgcc gatcaaaact ccgaattcat
     1441 tgcgaatcat gctaatcggt tcttccgcca ggccactgcc taccagtgca gcgttgacga
     1501 gaccgtttct accaagcagt gtcagccaag aaaaagctct gaccaagacc gagacccaga
     1561 aggaaatcag tacgagtgtc agcatccagt ttcgctctcg tccaaccaga tgaaccatgg
     1621 cataggcaat cagatagccg atcaccacgc tacacagtgt cgtgatgagg catactcgta
     1681 ttgtagtcca gagaatcttg accagcgatg actttgtgaa tagtagcttg taattttgta
     1741 tacctagttc cgggtcagaa actccaagcc aaagaatatt tattaccggc caagcataga
     1801 gagctatgat cagcccaaac aatggtacca cgagcaacca gtatgcgttc gacgcattac
     1861 tggttttcca attaaatatt atcgggccga tcgtcctact cttgcacgtc gttagccaat
     1921 ggcttcgaga aacctattga cggcatttcc accattttcc gcccaccaaa taggatcgtt
     1981 aaggagaact cgcgaaagat tctcttccga agtcactgcg aaacgcttga ggtcgtctgg
     2041 gatcattccg aaggcttcgg ggttagatgg agtcattcca tgacatcgaa gaatttcgat
     2101 ttgacgttca gctacttggc acgaggcaat aaatttcatt acatttgcac gtccagccgg
     2161 attcccctta ggaacgagat aagctccggg cattgcaatc gcctcattca tgttgagtcg
     2221 atagcgtccg ttggtgtcag cctctaccgc ctttgcgcgg ttgagccaga ccatgcccat
     2281 agaaacttca ccattcacaa tcatttgttg tgcttcagaa ccagaacccc agaagattgt
     2341 atctgatttg atcgacttga tcttgttgat cgcacggtca agatccattg gatagacctt
     2401 gtcttttgac acgccatcgg ccatcagggc accttcgagc gcgccattcg cccatttata
     2461 caaggatcgc ttgcccggat acttcttggt attgaaaaaa tccgcccaag tgctcggtgg
     2521 gttacctttg aatttttttg tatcgtacat gaacgcgtaa ccataaaaga tgatcgagac
     2581 tccgtaatcc caagcgaagc cctcgagtac ctttttcttg tcaacgacgg aataatcaat
     2641 tttctccaaa tgaccggact tgcctagtga aattgcatcg aaggcatcgg catctgcgac
     2701 atcagcggta acatttccgg aattcaccat ttccttgatt tttccttgta acggtcctga
     2761 ggtgtcatat ttgactggaa gaccggattc ttttgtgaat gcggcaccaa aagcactagt
     2821 atggcatttg acggcatcgc cgccccagtt ccacaacaca atttcgtttg cagccgcttc
     2881 agcatcgccg gcgatcacgg atgatgaggc aactccggcc aaggcacaaa ggtacaagaa
     2941 ttcgcgccta cccaacttac cgctcttgaa gtccttcgcc cctgtgaatg ccaggtcttt
     3001 ataaaacatc ttgtcttgca tatgtcttct ccttatttag gatttttaaa aaattgattt
     3061 tatagtaaac aattcactga accgtttaca atctgcataa acaacgcatt gagcaagtga
     3121 tacttgaaca ggctcagcgg agttgcttta cgggattcaa tctatctttc attattgtta
     3181 aagtcaatta aaaaaacctg tttttgtttg atggtctcgt aaaaagtcca actttagccg
     3241 ttataccatt cataatttat tgatataatt agtaattata ttattgaatt tgatttttta
     3301 cgatgcaatc ttatgtggaa ataagattgc caaatgactt tttttcttta aatgaagatt
     3361 caatatcttt aactgtaaaa gctgcatccg gtgaccatgt
//
LOCUS       DTVD01000010            3392 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_10788_length_3392_cov_197.220258, whole genome
            shotgun sequence.
ACCESSION   DTVD01000010 DTVD01000000
VERSION     DTVD01000010.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_3392_cov_197.220258"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..3282)
                     /locus_tag="EYO68_00325"
     CDS             complement(<1..3282)
                     /locus_tag="EYO68_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase"
                     /protein_id="HIC06283.1"
                     /translation="MTAPSFRFSIDRGGTFTDVYAEVPGEPGFRVVKLLSEDPQNYPD
                     APREGIRRILESVTGKHIPKASGGGSTTFSSENIEWIRMGTTVATNALLERKGARTVL
                     VTTKGFRDLLQIGNQSRPKIFDLEIRKLDLLYEEVIEVDERVRIFRETVKGSSRNAPA
                     SIVEGTTGEKFEVLSKPNLKEVSRQLEAVFKTGIRAVAVVFLHAYAFQEHERQIGELA
                     REIGYEQISLSHQVMPMVKMVARGDTTTVDAYLTPHIRNYLESFRSGFSDNLENSQLL
                     FMQSDGGLTDSENFKGSNAILSGPAGGVVGYAMTSSLTNEDTEISNSNQQQPKTGRRF
                     PVIGFDMGGTSTDVSRYGDDYELVHETETAGVRIQAPQMYIKTVAAGGGSRLFFRNGL
                     FEVGPESAGAHPGPVCYRKGGHLAVTDANLVLGRLHPEYFPKIFGSNENEALDLEASR
                     FAFEKLTAEINNYSKKRQLPEMSVEEVALGFLRVANEVMVRPIREISVMRGFDIKEHA
                     LACFGGAGGQHACAIARELGISKIFIHRFAGILSAYGMGLADIVVEIQEPSVLVLAES
                     SQDKSLKILLKKLDKLAENARTDLVEQGYKPEQIEIKRYLNLRYQGTDTALMVPEPEP
                     KKNYDTGITNCGLGIAESSPELQKNVQQSPSELPIPDFIGAFRETYRREFGFDLTGRE
                     IIVDDLRVRAVAKSPGLQQFTIAENSTDSQGRDEVPASKQTRCYFYGGWFDTPIYRLE
                     KLGSGLILQGPAILMQDTSTILVEPGCSAEITEYGDVVLSVKTKTYREIGTQADPVQV
                     SIFGNLFMSIAEQMGRTLQRTAISTNIKERLDFSCAIFDETGGLVANAPHQPVHLGAM
                     SEAVRQQVKLQENNLQEGDVLLTNHPIAGGSHLPDITVITPVWKKVSKFQSFKVSEKA
                     EKLKEEKIIFYIASRGHHADIGGISPGSMPPFSRELKEEGACIKTFKVVENGIFNEDG
                     ITELLQAPGKIVRNPGEPAISGTRLLSDNLSDLKAQVAANQRGIDLLLEMVEHYSSED
                     VPGLPIVKAYMHHIQNNAEEAVRNMLKELSEREGLAEVDTVAAKDYLDDGSEIVLHLT
                     IDRRDG"
BASE COUNT          870 a          837 c          713 g          972 t
ORIGIN      
        1 gccgtctctt cgatctatag tgagatgcaa aactatttcg ctgccgtcat ccagataatc
       61 ctttgctgca accgtatcta cttccgccag accttccctt tcagagagtt ccttgagcat
      121 attacgtact gcttcttcgg cattattttg gatatggtgc atatatgcct ttacaatcgg
      181 caggccaggc acatcttccg atgaatagtg ctctaccatt tccaaaagca aatcaattcc
      241 gcgctgattt gcagcaactt gtgctttgag atcggaaagg ttgtccgaaa gcaatcgggt
      301 tccgctgatc gccggctccc ccggattacg tactattttt ccgggcgctt ggagcaattc
      361 ggtaattccg tcttcattga aaattccgtt ttccaccact ttgaaagttt tgatacaagc
      421 accttcttct ttcaattcac gggaaaaagg tggcatcgat cctggagaaa ttccgcctat
      481 atctgcatgg tgtcctctac tggcaatgta aaaaattatc ttctcctctt ttagtttttc
      541 tgccttctct gaaactttga aactttgaaa ctttgaaact tttttccaaa ccggagtaat
      601 gactgtaatg tccggaagat gacttcctcc tgcaatcgga tggtttgtca gcaaaacatc
      661 accttcctga agattgtttt cctgcagttt aacttgttgt cggactgctt cgctcatggc
      721 accaagatga accggctggt gtggtgcatt tgctaccaat cctccggttt cgtcaaaaat
      781 agcacaggaa aaatccaggc gctccttgat gttggtggaa attgcggtac gttgtagggt
      841 acgtcccatc tgctctgcaa tcgacataaa aaggttaccg aaaatggaca cctgaaccgg
      901 atcagcctgg gtgccgattt cacgataggt cttggtctta acgctcaaaa ccacgtcacc
      961 gtattccgtt atttctgccg aacaacctgg ttcaaccaga attgtggaag tatcctgcat
     1021 taaaatcgca gggccttgta aaataagtcc ggaccccaat ttttccaggc ggtaaattgg
     1081 agtatcaaac cagcctccgt agaaataaca tcgggtttgt tttgaggcgg gcacctcatc
     1141 ccgaccttgt gagtcggtgg aattttctgc aattgtgaat tgttgcaggc ctggagactt
     1201 tgccaccgca cgtacacgca aatcatccac aatgatttct ctccctgtca aatcaaaacc
     1261 aaactctctg cggtatgttt ccctgaaggc accgataaag tcaggaatcg gaagctccga
     1321 tggagactgt tgaacatttt tctgcagttc aggactcgat tcagcaatgc ccaatccaca
     1381 attcgtaatt cctgtatcgt aatttttttt aggctccggt tcggggacca tcaatgccgt
     1441 atccgtaccc tgatagcgta aattgaggta gcgctttatt tcgatttgct ccggcttgta
     1501 tccctgctct actaaatctg ttcttgcgtt ttctgctaat ttatcaagtt ttttcaggag
     1561 gatttttaat gatttatcct ggctgctttc cgccagcacc agcacagaag gctcctgtat
     1621 ttctacgaca atatctgcca gccccattcc gtaagctgat aaaataccgg cgaaacggtg
     1681 aatgaaaatt ttggaaatac ctaactctct ggcaatggcg caagcgtgct gaccaccggc
     1741 accaccgaaa caggccaagg catgttcctt gatgtcaaag ccgcgcatca ctgaaatttc
     1801 acgaatcgga cgtaccatga cttcattggc aactcttaaa aaaccgaggg cgacttcttc
     1861 cacggacatt tccggaagtt gccgtttttt ggaatagttg ttgatttcag cagttaattt
     1921 ttcaaaggca aaacgtgagg cttctaagtc gagagcttcg ttttcatttg atccaaatat
     1981 ttttggaaaa tattcaggat gtaaacgtcc caataccaga tttgcgtctg ttacagccaa
     2041 atgcccgcct ttgcggtagc agacgggacc aggatgtgct ccggcggatt ccggaccaac
     2101 ctcaaacaaa ccattgcgaa aaaacagtct cgacccccct ccggcggcaa cggttttgat
     2161 gtacatttgt ggagcctgga tacgaacccc tgcagtttct gtctcatgca caagttcata
     2221 atcgtcaccg taacgggaaa catcagtcga ggtgccaccc atgtcaaaac caatcacagg
     2281 gaaccggcgg ccggtttttg gttgttgttg gttcgagttc gaaatttccg tgtcttcatt
     2341 cgtaagtgac gaagtcatgg catagcctac aactcctccg gcgggtccgg ataatatggc
     2401 attgctgccc ttaaaatttt cggaatcggt caaacctccg tctgactgca taaaaagcaa
     2461 ttgagagttt tccagattat ccgaaaatcc tgaacgaaaa ctttccaggt aattccggat
     2521 atgcggtgtg aggtaggcat ccacagtagt cgtgtcaccg cgtgccacca ttttgaccat
     2581 tggcatgact tgatgcgaaa gagagatttg ctcataaccg atttcgcggg ccagctctcc
     2641 gatttgcctc tcatgttcct gaaaagcata agcatgcaaa aatacgactg caactgcccg
     2701 aattcctgtt ttgaaaactg cttcgagttg ccgacttact tctttcaaat taggctttga
     2761 caatacctca aatttttctc cagttgtgcc ctcaacaatt gatgcaggag cattgcgtga
     2821 cgatccctta actgtttccc tgaaaatcct tacacgctca tcaacttcaa tcacctcttc
     2881 ataaagcaaa tcgagttttc ggatttcaag atcaaaaatt ttagggcggc tctgattccc
     2941 tatttgcagt aaatcccgaa atcccttcgt tgtcaccagt acagttcttg cccctttacg
     3001 ttccaacagt gcgtttgttg ccactgtggt acccatgcga atccactcaa tattttctga
     3061 agaaaaagtg gtagatccgc cgccacttgc tttgggaata tgtttcccgg ttactgattc
     3121 aagaatccgg cgaatgccct ctcgtggcgc atcaggataa ttctgcggat cttcggaaag
     3181 taacttcacg acccggaatc cgggttctcc cggcacttcc gcataaacat cggtgaaagt
     3241 accgccacgg tcaattgaaa aacggaagga aggtgctgtc ataaaattaa tcagtaaatt
     3301 aacaggatat aaagagaagt ttacgcagaa attaacttac ccgttcattg aattgaaatg
     3361 agcccttata ttctctcgcg acgattttag ct
//
LOCUS       DTVD01000011           12783 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1099_length_12783_cov_382.262492, whole genome
            shotgun sequence.
ACCESSION   DTVD01000011 DTVD01000000
VERSION     DTVD01000011.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_12783_cov_382.262492"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="EYO68_00330"
     CDS             <1..255
                     /locus_tag="EYO68_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIC06284.1"
                     /translation="GAEQALMKNKKSLLAKGVTRVEGQFQNKDLVEICNQEGVRLGVG
                     RADCSAKELRHHIKEQNLAKTGGKDPQSKPVIHRNHLFLE"
     gene            259..1698
                     /locus_tag="EYO68_00335"
     CDS             259..1698
                     /locus_tag="EYO68_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIC06285.1"
                     /translation="MTVSQPAFNASNSIIVRLNIDNKIGMLASVTQTISEMHGVVGAI
                     DLVQPEAGVLVRDISIFTHDPDHGKQIVTAIRNLPGVEFINFSDRTFLIHLGGKISIR
                     NRIPVKTRDDLSMAYTPGVARICMDIYEDPEDAYKLTIKGNTVAVISDGTAVLGLGDI
                     GPKAAMPVMEGKAMLFKEFANIDAWPICLDTTDTEEIIRTVKAIAPGFGAINLEDISA
                     PRCFEIERRLREELDIPVFHDDQHGTAVVTTAALINALKFVDKKVEDIKVVVSGAGAA
                     GTACSKMILQLGVKNLIGCDSKGAIHPKRNDLNSSKRWFADSTNPNQDSGTLKDVIAG
                     ADVFVGVSAPNVINQEDVKCMAKDPIVFAMSNPDPEISPDEALPVVAVMATGRSDYPN
                     QINNVLCFPGIFRGALDCQASTINEEMKLATAHAIAGTVKESHLCADYIIPSVFDSSV
                     APKIAKAVREAAIKTGVARRKKSSQQRFS"
     gene            1791..2549
                     /locus_tag="EYO68_00340"
     CDS             1791..2549
                     /locus_tag="EYO68_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06286.1"
                     /translation="MRSVYFQQPLEHQIEVEGESWNQGEVVKGQLRIRNMSSKTVAVK
                     TSQIILAHGLKKAFKEGTGVPWEVLEKQVAAQDIALQAGSELTFGWNFNLSTDCPITD
                     KQGGLYLLFGGEEALTEGGWLNLQINLHPILQNYLQTFTTQFRFLEKYQKRKSEWTEV
                     KLIPPDSREYPNMDYVLCFLRIHDEQLEAHYRFKMSGLGRTGEKMTVTKKNRELEQSI
                     PPEKYLQPGGFPNRACFRENIDQALNIARPEVIF"
     gene            complement(2731..3543)
                     /locus_tag="EYO68_00345"
     CDS             complement(2731..3543)
                     /locus_tag="EYO68_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06287.1"
                     /translation="MSKNKLIFLTAFVGFVGLTVYALWKEVSRGTAQLQYSISGVILS
                     GPGARGIVKTDNAHVLLFDPETFELVASKILNPFLPPSTFSIGQDDASQPLSGSYRLL
                     VLTDKNGDPNLPTAGEVIGPLSQPLPLGTEGVKYSVDHPFQVFPAELLAVETDSPDTS
                     IRGTITVAPELQSKLGLEDRLVIMLFDPQQGRPVAIKMLPSFRPPQNFSIGQTNAMGG
                     QQLSGKYSLRILTDKNSQPFQSVPGEIIGRSQGLISLGTGDLEFELDQPYIR"
     gene            complement(3565..5100)
                     /locus_tag="EYO68_00350"
     CDS             complement(3565..5100)
                     /locus_tag="EYO68_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIC06288.1"
                     /translation="MGKDTGFLEFDKESPPLRPVDERVRDFQEYYTFLSEENLQRQAS
                     RCMDCGVPFCHMGCPLGNMIPDWNDLVYRGHWKEALAALHSTNNFPEFTGRICPAPCE
                     DTCVLNEHYTLDAAEKQQKVNVVTIEEIEKHIAERGWEEGWIQPQLPEKLTGKCIAVV
                     GSGPAGLAAAQQLRRAGHDVTVFEKDNRIGGLLVYGIPDFKLDKKVVTRRVEQLRAEG
                     IKFRTGVHVGVDYSVKELRNSFDAILLATGAQNARKLMVEGSDLEGVHYAMHYLPQRN
                     REVAGDSIPEEQKIESGNKKAVILGGGFTAADCLGNLNRQGADPNIHQFELVDMVPRP
                     TPVHKEANPDCRANILTEKIISDDSGRVKALQAVCVEWKWEAKGNSAPGRMTMQRVPG
                     SEFSVSTDLVFLALGFLGPELDGLLEGLGVELNVRVGEKPFTSEQVKKLLKNAQPMYD
                     IFSDENFMTSKDGVFVAGDANRGASLVVWAIWEGREAARCIDKYLMGTTSLPTTPQAE
                     VLV"
     gene            complement(5116..9618)
                     /gene="gltB"
                     /locus_tag="EYO68_00355"
     CDS             complement(5116..9618)
                     /gene="gltB"
                     /locus_tag="EYO68_00355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIC06289.1"
                     /translation="MYQYPEKQGLYDPQFEHDNCGVGFVVQIKNRQSHQIVEQGLSLL
                     CNLNHRGALGADPDTGDGSGILIQIPHQFFVEECQSSGFHLPESGEYGIASIFLPQDP
                     YARRHCGEVIESHIVEKGMDLLGWRDVPIDRGYIGKQALATMPAMRQLFMGHRRFENY
                     NQDRFENKLYVTRKAIRSSLQDVEGFMIISMSSRTIIYKGMLIPHQMGKFFIDLSDKR
                     LRSALALVHTRFPTNTFPRWDLAQPFRNLAHNGEINTLRGNINWMLGRRATLKSPLYE
                     DISELQPIIIPRGSDSACMDNVFEFLIQSGYSLPHAMMMMVPEAWEQNPEMTPEKQAF
                     YEYHEHLMEPWDGPASLTFTNGVQIGATLDRNGLRPSRYVVTKDDLVIMGSEVGAVEI
                     PPENIKFKGRLQPGKMFLVDTKEGRIIDDTELKANICDAQPYSQWLKENMLELSDLPK
                     PDQVPETDFESLLLRQKIFAYTTEDINLLLTPMFTNGVEASGSMGDDTPLAVLSDKPR
                     LLYDYFKQIFAQVSNPPVDAIREELVMSLTSRLGSERNILDPGANHAKMLKLFHPILS
                     NEELAQIKALDKQDFRSKTLSMHFDAASGVEGFSRALKELCEEAESAVNSGTTFVVLS
                     DRGAAKDQVPIPALLAVGAVHHYLTLKRVRYKTGIIVETGEAREITHFCLLVSYGAGA
                     INPYLAIETIEQMIQQKELPEELTLEKANQNYCKAIRKGMYKVFSKMGISTIQSYRGA
                     QIFEAIGLDEELIRDYFTGTPSRISGVGIREIAQESLLRHETALEKTPETREILSGGG
                     LYHWRRNGEFHQINPVMTNTLQRAVRKNSQDAYNEFSRLVNEQKQRFSTPRSLFEFAE
                     GTPISLDEVEPAAEIVKRFVTGAMSLGSISSESHETMAIAMNRIGGKSNSGEGGEDSA
                     RFEKRTNGDFPNSTIKQVASGRFGVTIHYLVNCTEIQIKVAQGAKPGEGGQLPGNKVS
                     EEIAKIRNSTPGVTLISPPPHHDIYSIEDLAQLIFDLKNANPQAKINVKLVAEAGVGT
                     IAAGVAKAHADVITIAGHDGGTGASPLTSIKHAGVPWELGLSEAQQTLMLNQLRGRVR
                     LQTDGQLKTGRDVAVAALLGAEEFGFATVPLIAIGCIMMRKCHLNTCPVGIATQNPEL
                     RKKFVGKPEHVINYFFFVAEELRNIMAKLGFRKVDEMIGRTDMLKQRTNVNHWKAGKV
                     DLSAILHQIPVGEKDTPYCTQKQDHGLDKQIDLKLIAESKDALEHKKAVEFVLPINNV
                     NRSVGTMLSSHIAKKYGAEGLPDNTIHCKFVGSAGQSFGAFLAHGVTLELEGDANDYT
                     GKGLSGGRLVVYPPKKSTFSASENILIGNTVLYGATGGEVFFCGIAGERFAVRNSGVI
                     AVVEGVGDHGCEYMTGGRVIVLGETGKNFAAGMSGGIAYVLVENQSFHSRCNTEMVEL
                     EIVSELQEQKWLRKWIERHQDYTKSYRAASLLENWEKTLSQFVKVMPIEYRAVLEKMK
                     NKSSIK"
     gene            complement(9877..11646)
                     /locus_tag="EYO68_00360"
     CDS             complement(9877..11646)
                     /locus_tag="EYO68_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIC06290.1"
                     /translation="MDKNTLLAVVLSGAIMVGWYAMFPPPDPPPREIVNSVDQEYTDR
                     TKNSVRQESAELNVEDSSLSSTVPSSSIKEVDSSLPSKEVTIETENYRLVLDTKGGIG
                     KSLQLKHYKHTKPRLTLSTWFPFLTSFIGPDYLDEVTVDNRVQMFGNHLEGVPAFAQE
                     FKGDAKTTEMFRNTVFSASQDHVVLEEGRLNTTLTLTSPIVNGLQMVKIFEANPDSYI
                     LNYRIQLINRSNDVRPLEVLYFFGEQRLADNGGGMQQVSHEGPVFFFDESLQTESTEN
                     IENELPVTQMKWLGVEDQYFIGAAVPMTPVKNGFFRAGSYISEPQANQLGERKLSPYF
                     GVALPKTNLQPNLLVESDFRMFYGPKEDQELLKFGHNLEMSHDMTLEILAAPLLDLLR
                     LIYGYVGNYGVAIIILTIIVRVVLFPLTFKGMKSMKRMQQLTPRMKKLQEKYKKNKEK
                     LNKEMMELYRKNKVNPLGGCLPMLLQIPVFFALYSSLSSAVELRHAPFIFWISDLSQP
                     DGLGITPLLMGASMFIQQKMTPQTAMMDPTQAKIMQMLPFIFTIFTFTFPSGLTLYWV
                     TSNILSIAQQQIINRIKTPELQH"
     gene            complement(11639..11926)
                     /gene="yidD"
                     /locus_tag="EYO68_00365"
     CDS             complement(11639..11926)
                     /gene="yidD"
                     /locus_tag="EYO68_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIC06291.1"
                     /translation="MFLRFILSLIKVYRSCVSPFLPASCRFYPTCSAYALESYQTLGL
                     MRGTYLSLWRILKCNPLHPGGVDPVPGKSGPEPADNAVISERNLHKLKIYG"
     gene            complement(12009..12371)
                     /gene="rnpA"
                     /locus_tag="EYO68_00370"
     CDS             complement(12009..12371)
                     /gene="rnpA"
                     /locus_tag="EYO68_00370"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_602916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIC06292.1"
                     /translation="MSIKETYPKTIRLHHSQTIREVFVQGIYQSLGPIGVKYKIVEAE
                     CSRFAISVKKKVGSAPCRNRIKRLLREAIRKERLRLNCAYDICFFITSPPKSPLDSSY
                     VLCLVRQFFKNLNKESSE"
     gene            complement(12368..12502)
                     /locus_tag="EYO68_00375"
     CDS             complement(12368..12502)
                     /locus_tag="EYO68_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIC06293.1"
                     /translation="MKRTFQPNNRRRKKKHGFRARMATPGGRRVIKRRRAKGRKRLSA
                     "
BASE COUNT         3644 a         2938 c         2530 g         3671 t
ORIGIN      
        1 ggagcagaac aagctcttat gaaaaataaa aagagtttat tagccaaagg tgtaacacgc
       61 gttgaaggac aatttcaaaa caaggacctt gttgaaatat gcaatcagga aggtgtaagg
      121 ctcggagttg gaagagccga ttgttctgcc aaagaattgc gtcatcatat aaaagaacaa
      181 aacctggcaa agaccggtgg gaaagatccc cagagtaaac cggttattca cagaaatcac
      241 ctctttttgg agtaatttat gacagtcagc caacctgctt ttaatgccag caacagtatt
      301 atcgttcgtc tcaatattga taataagatt gggatgttag ccagtgtgac tcagaccatt
      361 tccgagatgc atggagtagt aggagcgata gacctcgtgc agcccgaagc cggggtttta
      421 gttcgtgaca tcagtatttt tactcatgat cctgatcacg gcaaacaaat cgtaacagca
      481 atcaggaatc tccccggagt tgaatttatc aacttttctg accgcacatt tttgatccat
      541 ttgggtggaa aaatcagtat ccgaaaccgt attcccgtca aaacccgtga tgacctttcc
      601 atggcctata ctccgggagt ggctcgaatt tgtatggata tctacgaaga tcctgaagat
      661 gcatacaaac tgaccatcaa aggcaacaca gtcgcagtga tttccgatgg tactgcagta
      721 ttaggacttg gtgacattgg ccctaaagcc gccatgccgg ttatggaagg caaagccatg
      781 ctttttaaag aattcgccaa catagacgca tggccgattt gcctcgatac cacagataca
      841 gaagaaatta tacggactgt taaggccatt gctcctggct ttggtgccat aaatttagaa
      901 gacatttcag cgccacgctg ctttgaaatt gaacgcagac tccgggaaga gctggatatt
      961 ccggtttttc atgatgatca acatggaact gccgttgtca cgactgccgc tttaatcaat
     1021 gctctaaagt tcgttgataa aaaagttgaa gacataaagg tagttgtttc cggtgcaggt
     1081 gcagctggaa ccgcatgttc caagatgatt ctacaacttg gtgtaaaaaa cttgattggc
     1141 tgtgacagca aaggtgcaat acatcctaaa cgaaacgatc tcaattccag taaacgctgg
     1201 tttgctgaca gtacaaatcc aaatcaggac tctggaactc tcaaagatgt aattgcagga
     1261 gcagatgtct ttgtgggggt ttccgctccc aatgtgatca atcaggaaga tgtaaaatgc
     1321 atggccaaag atccaattgt ttttgcaatg tcaaatcctg accctgaaat cagtccagat
     1381 gaagccttac ctgtagtggc cgtgatggca accggacgca gtgattatcc aaatcaaatc
     1441 aataatgtcc tctgttttcc gggaatattc cgtggagctc ttgattgtca ggccagtacg
     1501 ataaatgaag aaatgaaatt ggccactgca cacgccatcg ccggtactgt aaaagaatca
     1561 cacctctgtg cagactatat tattccgagt gtatttgact catcggttgc acctaaaatt
     1621 gctaaagctg tacgggaagc cgctataaaa actggggtcg caagaaggaa gaaaagttca
     1681 cagcagagat tcagctaagg tttacgggaa tctaaactaa taatcacatc cggcctgacc
     1741 taaatttcca tgttttccag gcctttaaaa cagcaaatta aagagatcaa atgagaagcg
     1801 tatattttca gcaacctctg gaacatcaaa ttgaagttga gggagagtca tggaatcagg
     1861 gagaagttgt aaaaggtcaa cttcgaatca ggaacatgag cagcaaaact gttgctgtaa
     1921 aaacttccca aattattctt gcacatggtt taaaaaaagc tttcaaagaa ggtacggggg
     1981 ttccctggga agttctcgaa aaacaagtag cagctcagga tattgcactg caggccggct
     2041 ctgaactaac tttcggatgg aattttaatt tatctacaga ctgtccaata actgacaagc
     2101 agggaggatt atatctgctt tttggcggag aagaagcact aactgaaggt ggatggctca
     2161 atttacagat taacctgcat ccaatcctgc aaaattattt gcaaactttc acgactcaat
     2221 ttcgtttttt ggaaaaatat cagaaacgaa aatcagaatg gactgaggtc aagctcattc
     2281 ctccagattc acgggaatat cccaacatgg actacgtgct ctgtttcctc cggattcatg
     2341 acgaacaact ggaggctcat tatcgattca aaatgagtgg actcggacgt actggagaaa
     2401 agatgaccgt cacaaaaaag aatcgtgagc ttgagcagag catacctccg gaaaaatatc
     2461 ttcagcccgg aggtttccct aaccgtgcct gctttcgtga aaatattgat caagctctca
     2521 atatagccag accggaagta attttttgat caggtttgcc gctttcaatt catctccatt
     2581 atcactccgg acaaagcgca gaaaagacct ggagtccatg aatacagttt aaaatcctgt
     2641 gctcaatctg atcgtgaatc tgaatcccaa atcaagtccg gaatgacaaa ataaatactt
     2701 tttcagtaaa gcctaattat tgccggtgga ttagcgaata tagggctgat ccagttcaaa
     2761 ctccaaatca ccggttccca aagaaatcag accttgtgaa cgacctatta tttctccagg
     2821 aacagattga aacggctgac tgtttttatc agtaagaatt ctgagagaat actttccact
     2881 gagttgttga cctcccatag cattagtttg gccaatactg aaattttgtg gtggcctgaa
     2941 actcgggagc attttgatgg caacaggacg gccttgttgt ggatcaaaga gcatgatcac
     3001 caatctatct tctaaaccta gtttactctg caattcagga gcaacggtga ttgtgccccg
     3061 gatactcgta tctggagaat ctgtctcgac agccaaaagt tccgcaggaa aaacctgaaa
     3121 agggtggtcc acagaatatt ttactccctc agttcctaaa ggtaacggct gagatagagg
     3181 cccaatgact tctccagctg taggaagatt tgggtcccca tttttgtcgg tgagtacaag
     3241 caggcgataa gagccgctga gcggttgact tgcgtcatcc tgtccgatac taaatgttga
     3301 aggcggtaaa aaagggttga gaattttcga agcaaccaat tcaaatgttt ccggatcaaa
     3361 gagcaaaaca tgagcattgt cagttttgac aattcctctt gctcccggac cggaaaggat
     3421 cacaccgctg atgctgtact gtaattgagc agtgccgcgg cttacttctt tccagagagc
     3481 ataaacagtt aggcccacaa aaccaacgaa ggcagtcaaa aatataagtt tgtttttgct
     3541 cacaatattt agttttaacg attgttatac cagcacttct gcctgaggtg tcgttgggag
     3601 acttgtggtt cccatcaaat atttatcgat acaacgtgca gcctcacgcc cttcccaaat
     3661 ggcccaaact acaagcgagg caccacgatt tgcatcacct gctacgaaca caccgtcttt
     3721 actggtcata aaattttcat ctgaaaaaat atcatacatc ggctgcgcgt ttttcagcaa
     3781 ttttttaact tgctcagatg taaaaggttt ttctcctaca cgaacattca actcaactcc
     3841 cagtccttcc agtaatccat ccaattctgg ccccaaaaat cccaatgcca gaaaaaccaa
     3901 atcagtggac acgctaaact ctgaccccgg aacacgctgc atcgtcattc gaccaggcgc
     3961 agagtttccc tttgcttccc atttccattc tacacaaacc gcctgcaaag ctttaacccg
     4021 gcctgagtcg tcgctgatta tcttttccgt caaaatattg gctctacaat ccggattcgc
     4081 ttctttatgc actggagtag gacgcggcac catatcaacc agttcaaact gatgaatgtt
     4141 aggatctgca ccttgccgat ttaggttacc caaacaatca gctgctgtaa atccgccgcc
     4201 gagaattact gcttttttgt ttccggattc gattttttgc tcttccggaa tggaatcccc
     4261 ggcgacttca cgattgcgct gcggaagata gtgcattgca taatggactc cttctaagtc
     4321 actgccttcg accatcaact ttcgcgcatt ttgcgcaccg gttgccaaca ggatagcatc
     4381 aaaactgttc cgcagttctt tgactgaata atccacgcct acatggactc ctgtccggaa
     4441 cttgatcccc tctgcacgga gttgttcaac gcgtcgggta accacttttt tgtccaactt
     4501 gaaatccgga ataccgtaaa ccagtaatcc acctattcta ttgtcttttt caaaaacagt
     4561 cacatcatgt cctgcacgcc gcaactgctg tgccgctgcc agtccggcag gaccggaacc
     4621 cactaccgca atgcacttac cggtaagttt ttcaggcagt tgaggttgta tccagccctc
     4681 ttcccaacct cgctcagcga tgtgcttttc aatttcttca attgtgacaa cattcacttt
     4741 ttgctgtttt tctgctgcat ccaaagtgta atgttcattc aaaacacaag tgtcctcgca
     4801 tggtgcaggg caaatacggc cggtaaactc cgggaaatta ttggtgctgt gtaatgcagc
     4861 cagagcttct ttccagtgtc cacgataaac aagatcattc cagtcgggaa tcatgttgcc
     4921 caggggacag cccatgtgac aaaacggtac accgcaatcc atgcagcggg aggcctgacg
     4981 ctgcaaattt tcttcagaca aaaaagtgta atattcctga aaatcacgca ctctttcatc
     5041 cactggacgc agcggcgggc tctctttatc aaattctaag aaacctgtat cttttcccat
     5101 ttgttaatac tgctgctatt taatcgagga tttatttttc attttttcca ataccgcccg
     5161 gtattctatc ggcattactt ttacaaactg acttaatgtt ttttcccagt tttccaataa
     5221 acttgccgca cggtaacttt ttgtatagtc ttgatgtcgc tcaatccatt tccggagcca
     5281 tttttgttcc tgcagttcac tcacaatttc caattcaacc atttcagtgt tgcagcgtga
     5341 atggaatgat tggttctcta ccaaaacata agcaatccca ccactcattc ctgcggcaaa
     5401 attctttccg gtttctccaa gcacaattac ccttccacct gtcatgtact cacaaccatg
     5461 gtcgccaaca ccctcaacca ctgcaattac gccactgtta cggacagcaa agcgttcgcc
     5521 tgcaataccg cagaaaaaaa cttctccgcc agttgctcca tacagtacag tattgccaat
     5581 taaaatattt tcagaggcac tgaatgtaga ttttttggga ggataaacaa ccagccggcc
     5641 gccgctcaaa ccttttcccg tgtaatcatt ggcatcaccc tcaagctcaa gagtaacacc
     5701 atgagccagg aatgctccaa aactttgccc tgcagaaccc acaaatttgc agtgaattgt
     5761 gttatccggc aaaccttctg caccgtattt tttggctata tgactactta acatagtacc
     5821 gactgagcgg ttcacattgt tgatcggtaa cacaaactct actgcctttt tgtgttccag
     5881 cgcatcttta gattctgcaa tcagcttaag atcaatttgt ttgtccagtc cgtgatcttg
     5941 tttctgagta cagtaaggag tgtccttttc tcccacagga atctgatgca ggattgctga
     6001 taaatcaact tttccagctt tccaatggtt tacgttcgtg cgctgcttca acatatctgt
     6061 tcgtcctatc atttcatcaa ctttgcgaaa acccagtttg gccatgatat tccgtagttc
     6121 ttctgctaca aaaaagaaat agttgatgac atgttccggc ttgccaacaa actttttacg
     6181 taattcagga ttttgtgtgg caataccgac cggacacgta ttgaggtgac atttacgcat
     6241 cataatgcag ccgatagcaa tcaatggaac agtcgcaaaa ccaaattctt ctgcacctaa
     6301 caaagccgct accgctacat cccttccggt tttaagttga ccgtcggtct gtaaacgtac
     6361 ccgaccgcgc aattgattca acattaatgt ctgctgtgct tcagaaagcc caagttccca
     6421 cggcactccc gcatgtttta tcgaggtcaa aggtgacgca ccagtcccgc cgtcgtgacc
     6481 agcgattgtg atgacatcag catgagcttt tgctactccc gcagcaatgg taccaactcc
     6541 tgcttctgcc accagcttaa cgttgatttt tgcttgtgga ttagcatttt tcagatcaaa
     6601 aattaactga gccagatctt cgatagaata aatatcgtgg tgcggaggcg gtgaaatcaa
     6661 ggtcactccg ggagtggaat tgcgaatttt tgcaatttcc tcactgactt tattcccagg
     6721 tagttgaccg ccttctcccg gcttggcacc ctgagcgact ttaatttgga tttctgtaca
     6781 gttgaccaga taatggattg taactccaaa acgtcctgaa gcaacttgtt taatagtact
     6841 gtttgggaaa tctccgtttg ttcgtttttc aaaacgtgca gaatcttcac cgccttctcc
     6901 actgttgctt tttccaccga tgcgattcat ggcaatcgcc atagtttcgt gtgattcaga
     6961 gctgatcgat ccaagtgaca tcgctccagt cacaaatcgc tttacaattt ccgcagcagg
     7021 ttcaacttca tcaagcgaaa ttggagttcc ttctgcaaac tcaaacaagc ttctcggtgt
     7081 agaaaaacgc tgtttctgtt cattgacgag acgtgagaat tcattataag cgtcctgact
     7141 gtttttgcgg acagctctct gtagagtgtt tgtcatgacc ggattaattt gatgaaattc
     7201 tccgtttctg cgccaatgat ataatcctcc tccagacaat atttctctag tttccggagt
     7261 tttttccaaa gccgtttcat gcctcaataa gctttcctgg gcaatttctc ttataccaac
     7321 accgcttatt ctggatggag ttcctgtaaa ataatcccgt atcagttctt catccagtcc
     7381 aatcgcctca aaaatttgag cgccacgata gctttgtatg gtagaaattc ccatcttcga
     7441 gaaaacttta tacattcctt tacgaatggc cttacaataa ttctgattag ctttttccag
     7501 cgttaattcc tcaggcaact ccttttgctg aatcatttgt tctatagttt caatagccag
     7561 atacggattg atcgcacctg caccataact tacaagtaag caaaaatgtg taatttccct
     7621 cgcttctccg gtttccacaa ttatacccgt tttgtagcgt acccttttga gagtcaggta
     7681 atggtgaact gcccctactg ctaataaggc aggaattggg acttgatcct tagctgcacc
     7741 acgatcactc aaaaccacaa aagttgtacc ggaattcact gcactttctg cttcttcaca
     7801 aagttcttta agcgctctgg aaaacccttc aacacctgaa gctgcatcaa aatgcatact
     7861 taaagttttc gatctgaagt cctgtttatc gagtgcttta atttgtgcaa gctcttcatt
     7921 gctcagtatt ggatgaaaga gtttcaacat ctttgcgtga tttgctccgg gatcaagaat
     7981 atttctttca gaccccaacc tgcttgtcag tgacatcacc aattcttccc ggattgcatc
     8041 aacgggagga ttactaactt gtgcaaaaat ttgcttgaag taatcataaa gcagccgcgg
     8101 cttgtctgag agcacggcta acggcgtatc gtcacccatt gaacctgaag cttcaacacc
     8161 gtttgtaaac attggagtta ataataaatt tatatcttcg gtggtataag caaaaatttt
     8221 ctgccttaat aaaagtgatt caaaatccgt ttccggtacc tgatccggtt ttggcaaatc
     8281 agacaattca agcatattct ctttcagcca ctgcgaataa ggttgagcgt cacaaatatt
     8341 agctttcaat tcagtatcat caataatcct tccctctttg gtatcaacaa gaaacatctt
     8401 gcccggctgt aaacgccctt taaatttgat attttccgga ggaatctcga cggctcctac
     8461 ttcagagccc ataatgacca gatcatcttt tgtcactaca tatcgtgacg gacgcaatcc
     8521 gttacgatcc agtgtcgctc caatttgaac accatttgta aaagtgagag atgccggacc
     8581 atcccagggc tccattaaat gttcatggta ttcataaaat gcctgttttt caggagtcat
     8641 ttccgggttt tgctcccatg cttccggcac catcatcatc attgcatgtg ggagagaata
     8701 gccggattga atcaagaatt cgaaaacatt gtccatgcag gcggaatcac tgcctcttgg
     8761 aataatgatg ggctgcagtt cgctgatatc ctcatacaat ggtgacttta aagtcgcacg
     8821 cctgcccagc atccagttta tattaccgcg cagtgtgttt atttctccat tgtgagccaa
     8881 atttctgaaa ggctgagcca aatcccagcg cggaaaggtg ttagtcggga aacgggtgtg
     8941 taccaacgcc aaagcactcc tcaagcgttt atcagataaa tcaatgaaaa atttccccat
     9001 ctgatgtgga atcagcatcc ctttataaat tatggtacgt gaggacatgc tgataatcat
     9061 aaaaccttca acatcctgca gtgatgaacg tatggcttta cgcgttacat acaatttatt
     9121 ttcaaagcgg tcctgattat aattttcaaa cctgcgatgc cccatgaaca attggcgcat
     9181 agctggcatt gtggcaagag cctgttttcc aatgtatcct ctgtcaatcg gaacatcacg
     9241 ccaccccagc aaatccatcc ctttttcaac aatgtgactt tcaatcactt cgccacaatg
     9301 tcttctggca taaggatctt gaggaagaaa aatggaggca atcccatact ctccggattc
     9361 aggcaaatgg aacccggaac tttggcattc ttcaacaaaa aactggtggg gaatttgaat
     9421 caaaattcca gaaccatctc ctgtatctgg atctgcgcca agtgctcccc ggtgattcag
     9481 attgcagagt aaactcagtc cctgctcgac aatttggtga gattgccgat tttttatctg
     9541 aactacaaat cccacaccac aattgtcgtg ctcaaattga ggatcgtaaa gcccttgttt
     9601 ttcaggatat tgatacatat aggaaactaa aattatactt tataaaaagc tctgcactaa
     9661 actttatgaa gttatggttt tattcttttg gtttattcaa ccaactatta aaacaagtca
     9721 aaatatatca tgagtaggca aagcttttcg ctcatgaatg atcctttatt gacactataa
     9781 cttatagaga agaacagaaa ttctgaccaa aatcaagttt tctgctaacc aggaaaattg
     9841 agttttaagg aaaagactga aaaacagaac agctgatcag tgttgtaact cgggtgtttt
     9901 gatccggttg ataatttgct gctgggcaat cgaaaggata ttgctcgtta cccaatacaa
     9961 agtcaatccg gatgggaaag tgaaggtaaa aatagtaaaa atgaagggca gcatttgcat
    10021 gattttcgcc tgtgtggggt ccatcattgc ggtttgtggg gtcatttttt gttgaatgaa
    10081 catagatgcc cccatcagca agggcgtaat ccccaatccg tcaggttgtg acaagtcact
    10141 aatccaaaag atgaaaggtg catgtcgcaa ttcaacggca cttgaaagtg agctataaag
    10201 cgcaaaaaac acagggatct ggagcagcat cggtaaacaa ccaccaagcg gattgacttt
    10261 atttttacga tacagctcca tcatttcctt gttcagcttt tctttgtttt ttttatactt
    10321 ttcctgtagt ttcttcatcc tcggagttaa ctgctgcata cgcttcatac ttttcatgcc
    10381 cttgaaagtc agaggaaaca aaactacacg aactataatc gtcagaataa taatagcaac
    10441 accgtagttt ccaacataac cgtaaatcaa gcgtagtaaa tccaacagtg gtgctgccag
    10501 aatttccaga gtcatgtcgt gagacatttc cagattgtgc ccaaatttca gcaattcttg
    10561 atcttctttt gggccataaa acattctaaa atctgactca acgagaagat ttggctgtaa
    10621 atttgtcttc ggcagagcta caccaaaata tggagacaat tttctctctc ccaactgatt
    10681 agcttgtggt tcagaaatgt atgatcctgc tctaaaaaaa ccgttcttta ccggagtcat
    10741 tggaactgcc gcaccaataa aatattgatc ctcaacacct aaccatttca tttgcgttac
    10801 gggcagttca ttttctatgt tttcagtgct ttcagtctgc agactctcat cgaaaaaaaa
    10861 gactggacct tcatgagaaa cttgttgcat tccgccgcca ttgtctgcaa gccgctgttc
    10921 tccaaaaaaa taaagcactt ccaaaggacg aacatcattg gagcgattga tcaactgaat
    10981 acgataattt aaaatataac tatcaggatt tgcttcgaag attttaacca tctggagccc
    11041 gttcactatg ggtgaggtta aagtgagtgt agtattcagg cgtccttctt ccagcacaac
    11101 atgatcttga cttgcagaaa atacagtatt gcgaaacatt tcagtggttt ttgcatctcc
    11161 cttaaactct tgagcaaaag cgggtactcc ttccaaatga ttcccgaaca tttgtacacg
    11221 gttgtccact gttacttcat caagataatc tggaccaatg aagctggtaa gaaatgggaa
    11281 ccaggtactc aaagtaagcc tgggctttgt atgtttataa tgttttaact gcagactttt
    11341 tccaattcca cctttagtgt ctaaaaccaa cctgtaattc tctgtttcta tggttacttc
    11401 ctttgatggt aaactactgt caacctcctt aattgatgac gaaggtacag ttgaagaaag
    11461 ggaagaatct tcaacattaa gttcagcaga ttcttgtctc accgaatttt tggtcctgtc
    11521 tgtatattcc tgatctacgc tatttacaat ctctcgagga ggcggatccg gtggagggaa
    11581 catggcatac catccaacca taattgcacc tgagagtact actgccagta aagtgttttt
    11641 atccataaat tttaagcttg tgtaagtttc tttcagaaat taccgcatta tcagctggtt
    11701 ctgggccaga ttttcctgga accggatcaa cacctccggg atgcagcgga ttacatttca
    11761 agatccgcca taaactcaga taggtacctc tcatcaaacc cagagtttga taagattcca
    11821 gagcatatgc cgaacaagtg ggataaaacc ggcaggaagc aggcaaaaaa ggagatacac
    11881 aagatctata aaccttgatt aagctcagga tgaaacgaag aaaaatatta ctgttttcgt
    11941 tattcggtga tttcttgcaa caattctcaa aacttggtgc tacccgactt tgtattgcgt
    12001 tagcaggctc actcacttga ttccttgttt aagtttttga aaaactgtct gaccagacac
    12061 aagacataag atgagtccaa gggactcttg ggcgggctcg ttataaaaaa acaaatgtcg
    12121 tatgcgcaat tcaaccgtaa ccgttcctta cgaatcgcct ctctcaacaa acgtttgata
    12181 cgatttcggc aaggtgcact cccgaccttc tttttaacgg agattgcaaa acggctgcat
    12241 tcagcttcta ctattttata tttaacacca atcggcccca aagactgata tatcccctgc
    12301 acaaacacct cccgaatagt ttgactatgg tgtagcctga tcgtcttcgg gtaagtttcc
    12361 tttatgctca tgcactgaga cgttttctgc ctttagcgcg tctacgcttg attactcttc
    12421 gacctccagg agtcgccatg cgagccctga aaccgtgctt ttttttgcgt cgccgattgt
    12481 ttggctggaa agtgcgtttc attgttagat gtcttcaaat taaaaaactt cctaaaaaaa
    12541 ctaattgttc agtagaccct gtttacgtta ataatacaag ctttaatgat ttattgtatt
    12601 aatttggctc acaagaacta cataactaaa gcttagtttg gcaatttttt gaggcagatt
    12661 atcagagtga aattgaattc taaattagcg ccatcttggc agtcttacaa ctggacagca
    12721 ttcgattgca aatctggtcc agccgaaaag tacatttaat gttgttatgt ttaaagagta
    12781 gta
//
LOCUS       DTVD01000012           12717 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1113_length_12717_cov_330.989654, whole genome
            shotgun sequence.
ACCESSION   DTVD01000012 DTVD01000000
VERSION     DTVD01000012.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12717)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12717
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_12717_cov_330.989654"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            114..2227
                     /locus_tag="EYO68_00380"
                     /pseudo
     CDS             114..2227
                     /locus_tag="EYO68_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            2490..3635
                     /gene="arsB"
                     /locus_tag="EYO68_00385"
     CDS             2490..3635
                     /gene="arsB"
                     /locus_tag="EYO68_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIC06294.1"
                     /translation="MKKTTGVKVTEKKLSIFERYLTVWVLLCIVGGIILGKIAPDAAA
                     TLNEFSVYQVSVPIAVCLFFMMYPIMVKIDFSQVLKSAKTPKPVFLTLFINWAIKPFT
                     MFAIAYFFLGYLFVDYLPGTEILKNGEEVELWRSYVAGAILLGIAPCTAMVLMWGHLA
                     KGNAGLTLVMVAINSLIMLVIFGPLGGLLLNINSMPVPWETMLFSVSVYVAFPLIAGY
                     LSRKWILQYKGQEWFNEKFLHWLTPVSITALLVTLLLLFSFKGEVIVNNPLTIIWISI
                     PLFIQTVLIFVIGYFILARWMKLSYHDAAPAALIGASNHFEVAIATAVILFGLSSGAA
                     LATVVGVLIEVPLMLMLVSICKRTCHLFDECHLDYPNCKPVLVSNEI"
     gene            3841..4164
                     /locus_tag="EYO68_00390"
     CDS             3841..4164
                     /locus_tag="EYO68_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HIC06295.1"
                     /translation="MRKVYSSPKTNLVHFAKSVLEANNIESVILREQLAGVVGGIAPL
                     DAWPELWVHDAERLEEARQLLEASMAKSETLDKPWQCQGCGEKIEAQFTQCWKCGTEF
                     WLSES"
     assembly_gap    4240..4339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4366..5037
                     /locus_tag="EYO68_00395"
     CDS             4366..5037
                     /locus_tag="EYO68_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007622131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIC06296.1"
                     /translation="MGIPVHLLQRGNNRQDCFNMNGDYAAYLWWLREYSEKFQVEIHV
                     WSLMKNHIHLLCTPQLEGGLSRMIQALGRQYVRYFNYQNHRTGTLWEGRYRSCLVQPE
                     GYLLKVYRYIELNSVRKKYVSKPSDYHWSSYQINAQGKPSALCKPHAEYLKLGESKAE
                     RTEKYQEMFEQSSREDLLEEIRDSTNKGLAFGDESFKIEVEKMTGRRVRSLKSGRPLG
                     WRKPK"
     gene            5084..5842
                     /locus_tag="EYO68_00400"
     CDS             5084..5842
                     /locus_tag="EYO68_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIC06297.1"
                     /translation="MKKYPKKSKAVYFFGTCLVDLFYPEAGMAGIKLLQREGLEVIFP
                     QQQTCCGQPAFNSGFHDEARKVAAKQVVLFPKDIPIVIPSGSCGAMIRKHYAELFKND
                     DLKTEAESVAERIFELTEFLVYVLKIKLKDLGEPIKLTWHTSCHAKREMGIDTEPKEL
                     LRQLKNVELLELEREDECCGFGGTFAVKEPHISAAMVKDKVDDISKTGASRLLSGDCG
                     CLLNISGAMKHSGISTDHQHIAEFIWERTANNKS"
     gene            5857..6864
                     /locus_tag="EYO68_00405"
     CDS             5857..6864
                     /locus_tag="EYO68_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIC06298.1"
                     /translation="MNTEHMSLAEVESLATDALIGTGTRLESAQSMGRAARAAERDGI
                     RSHGLLYVPTYCEHVKCGKVDGTAVPEIIQPRLSAIKVDAKTGFAHPAIEAGFIELIP
                     LVKSNGCVGLTISNSYNCGVLGYHVERLAEAGLVGIGFTNSPASIAPVGGKKPVIGTN
                     PFALAVPDSAGGVAFVLDQSASVVAKSEIIMRAREGDSIPEGWALDAEGQTTTDPEIA
                     LKGSMAPSGGYKGFGTGLLVEVMAAALSGAMLGLQASPFSGTAGGPPRTGQCFLAFDP
                     NAYSGAEFAERITILTEAIQSQEGARLPGDRRKENRQRIEIEGVEVEKSLIKRIQTFC
                     T"
     gene            complement(6971..7198)
                     /locus_tag="EYO68_00410"
     CDS             complement(6971..7198)
                     /locus_tag="EYO68_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06299.1"
                     /translation="MIHKQKIFNILFLWTTLIFLQMHTGCSREPQYIIFKTGDRNQFQ
                     ERAIKYCYGDFKVLEEEEIGPYTRAHLECKE"
     gene            complement(7203..9395)
                     /locus_tag="EYO68_00415"
     CDS             complement(7203..9395)
                     /locus_tag="EYO68_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC06300.1"
                     /translation="MNLFEHAHDKQIRKEAPLADRMRPRTIEEFVGQSHILAPGRLLR
                     RAIQADQLSSLIFYGPPGTGKTTLARIIANTTKAEFLSINAVLSGIADIRKCIETAKK
                     VRTEHQRHAVLFVDEVHRFNKAQQDALLPHVENGTVILIGATTENPYFEVNKALVSRS
                     RIFQLQSLKSTEVEEIIEQALTDSERGFGDKQVNLAAEARRHLAHVAGGDARAALNAL
                     ELAVLTSQADENDVLQITLEVAEESIQQRAVLYDKDGDAHFDTISAFIKSMRGSDPDA
                     ALFWMAKMVEAGEDPRFIFRRMLIFAGEDVGMADPQALGVVSSAAQAFDYVGMPEGRY
                     HLAQACLYLSTAPKSNSAFAFFDAISVVRNEQADEVPDPLKDGNRDGKAFGHGEGYLY
                     PHAYRDHWVAQQYLPDVLQGRIFYQPSEQGFEASIQENVSRNREAQLAAFFSQTASEQ
                     SEGSIYWEARTLDSSGELLNEFRDHLTEWSEISRNTLALVLNAGEGLLLGEFLRRISE
                     ENVYALVESSNEKKILGTLFKKTTSGIEAKIAVDSSGCPASFEIADLRFERIVGRNVL
                     QKHADKSGFLETLKPWISADGFFVFGETVPALGQRLSDLIPEKLLEPDFRKKLKTAEK
                     GIYLDEENVRSSWTPSTLCDELKGANWNIIRWQVKEYCIPTMIRTEQIEQWFPSHQNS
                     PHSSYGQRLSAYFSPEQLNNLRETFRNEVAGTVVEWHSVSLFMQLNQK"
     gene            9524..11536
                     /gene="ligA"
                     /locus_tag="EYO68_00420"
     CDS             9524..11536
                     /gene="ligA"
                     /locus_tag="EYO68_00420"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIC06301.1"
                     /translation="MPFGSTTQARIRELSTLIHQYNYSYYCGNDSDESEKYCADETYD
                     ALVLELKALENQYPELKLEDSPTLHITGEPHSVFPSRKHLIPMLSLGNVYSLDELKSW
                     DTSIQKLLGGQNTEYVCELKIDGVAISAIYRNGLLSAGVTRGDGSEGEEITPNIKTIS
                     CLPLKINDERDLDVRGEVYFPRKNFDAYNQQRISSGELPFKNPRNAAAGSLRLLDSTE
                     TRRRNLAVFIYTIAEGAPEESHAGNLEFLQQQEFPVNPETKKVGSIEEVLEFCRYWEE
                     HKEELPYDIDGIVLKINSLKQQRQLGFTAKSPRWATAFKFTAEQAFSVLREVEVGVGR
                     TGNLTPVAILDPVELNGTIVGRATLHNYDQVERLNLHLGDHVTLEKGGEIIPKIVAVD
                     PTKRHSAAQKIKPPQCCPSCGTSAVRFQDEVEWRCPNSECPAQQKEQILHFVSRRAMD
                     MDTIGPALIEQLMTKNLLQNAADLYLLRHEDLSGLERMADKSAANVLSSIEKSKKCEL
                     AQFVHALGIANVGEKTASILAQHFGTLERLMSGTPDDFEKVEAIGPVIAESIIDFFQD
                     QRQRQLIADFLERGVSPAEVKILEIVDSPFSGKIVVLTGTLSEPRDVWKKRLIQAGAN
                     VTGSVSKKTDFVLAGENAGSKLEKAEKLEVAVIDETTALNLLEQIS"
     gene            11783..12667
                     /locus_tag="EYO68_00425"
     CDS             11783..12667
                     /locus_tag="EYO68_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIC06302.1"
                     /translation="MKGVILAAGYATRFLPASKTIPKEMFPLIDRPAIDFIVQEMVDS
                     GIHDILLVSSRRKKVMEDYFDREIELSSAFSKSNEIEKLEVIKPIEANIFTLRQQRMM
                     GTGNALMLVEPFVDNQPFVVAYPDDIVLGEKPLSRQLIDAWEKTGNTVLAVKELEEDQ
                     LSRYGVIDPADSGKIMQVNKMVEKPLPGTAPSRFVSYGRYLYTPDLFEALRRSDKSHS
                     HQSEFTQTEAINHLAAQGQVSALQFEGTRYDLGEPLGFLTSAMQIGLLRPEYKTALLD
                     EMRRLLKLHDNPDNENSG"
BASE COUNT         3725 a         2444 c         2821 g         3627 t
ORIGIN      
        1 caaaatgatg cagaactgat caagttttct ctgctttaat gttgtttcca tgtgttgtac
       61 tgtaaggtct gataacattc tctatccggc tcctggttat tagagactcc ttcatgaaaa
      121 acatctgtac aattatttta acttttttgt atttactaat tttcctcgta aatgcccaag
      181 gtaaggaacg acctgttcct gcagtacagc ataaatatac aaaccaactt atccacgaaa
      241 gcagtccata tttgctacag catgcccata atccagtgaa ctggtatcca tggggaaaat
      301 ccggttttga aaaagcagaa aaagaaaata aactgatcat tgtcagtata ggttattcat
      361 cctgccactg gtgtcatgta atggaagtgg aaagttttga aaacgaggaa atagcaaaaa
      421 ttatgaatga aaattatgtt gctattaaag tagacagaga ggaacggccg gacattgatc
      481 agatctacat gaatgctatt cagttgttga caggcagtgg cggctggccc ttgaactgta
      541 ttacactgcc aaatcgagaa cctttttttg cggaacttat ttcccaaaag aaaagtggct
      601 ggaaatccta attaaagtcg cagattttgt taagcaaaat cctgaaaaaa caaaattact
      661 tgcgaggcag gtcacagatg gtgtgcggtc cagtgaaata aattttgcca ctgatgaaaa
      721 gccggaattc agcataaaag atttgaatgg tgtctttgcc gcttggcaaa aaaatataga
      781 ttatctacag ggcggaagca aaggagaacc taaattccca atgccgattg gctaccaatt
      841 tttgctgcac tacaattttc ttgttaaaaa tccggatgta ttgaaggcag taacagttac
      901 tttggacaaa atggcagcag gaggtattta tgatcaggta ggaggaggtt tttcaagata
      961 ttctactgat cagtactgga aagtaccgca ctttgaaaaa atgctctatg acaatgcgct
     1021 tttggtaagc ctgtattccc gtgcctatca gcagacaaaa aatccaaaat acaaaactgt
     1081 tgttcaagaa acacttgctt tcataaaaag agaaatgact tctcatgaag gaggtttttt
     1141 ttcgtcacta gatgctgaca gtgacggtga ggagggtaag tattatgttt ggaaacttga
     1201 ggagttgaaa tacattttgg gccgggaagc tgattttata attgattatt acaatttgac
     1261 tttagaagga aattgggaag acaggaagaa tattttatat atttctgcaa ccgacaaaca
     1321 actggcagat aaatatcaaa tcactgtaag tgaattaaat aaccgagtat ttgcgtcaaa
     1381 agaaatatta ttgaaagaaa gatcagctag agttcatccc gggcttgacg acaaaattat
     1441 cacttcctgg aatgctctga tgcttaaagc atatattgat gcttttgagg ttttcggcaa
     1501 tgaggattat ttgactgccg cacttaagaa tgcagaattt attgccagta aattaaaatc
     1561 tgctgatcat agattaaata gaaattataa aaacgaaaag gcaggcatta acgcatattt
     1621 agatgattat gcctttagca tttctgcttt catttcctta taccaggtta cattttcaga
     1681 aaaatggtta gatactgcac gaaaattaac agactatgca attgatcatt tctttgaaaa
     1741 taaaagcgga atgttttttt acacctctga tcttgatccg gcactgattg caaggaagaa
     1801 agagataatt gataatgtta tgcctgcttc aaattcagaa atggcaaaaa atcttttcgt
     1861 tttggggtat tacttttaca atgaagaata tctggaaaaa tcctcaaaga tgcttaactc
     1921 agttaagaaa tatactttaa acggtggcag ctattttgcg aattgggaca ttttgatgtc
     1981 atggttctcc agtgaaccat atgaagtggc gattgttgga aaagaatttg agcatctgcg
     2041 gagagagttt aacggacact acctgccaaa tgtgtttttt tccggaggga gcagtgaagg
     2101 tactttgtct ttacttgaaa acaaactggt tgaaggggaa actacaattt atgtgtgtca
     2161 gttcaaagtc tgtaaacttc ctgtgactga tacaaaagct gcattgactc aaatctttga
     2221 tcagtaattg tgactcttcc ataaaaaata ttttccagta aagctggaat catgaaatta
     2281 tcaaatgaat taaattttag ataactgctc attgggtaac tcaaaaaaaa tcatgtccgg
     2341 aatgtctgaa aagtattgtt tactttatta aaatccagca aatatttaag gctcactgaa
     2401 aaagtttttc ttttgtcatc ccggatcttt gcttagcttc ccccggaatg acacggttta
     2461 gggactattt actgttttat caataattta tgaaaaaaac tacaggagta aaagtgacag
     2521 aaaaaaaatt atcaatcttt gaacgctatc ttacagtttg ggtattgctc tgcattgtgg
     2581 gcggaattat tctaggtaaa attgcacctg atgctgcagc aacgctgaat gaattttctg
     2641 tttatcaagt atctgttccc atagcagttt gtttgttttt catgatgtat ccaatcatgg
     2701 taaaaataga tttttcacag gttttaaaat cagcaaaaac tccaaagccc gtttttctga
     2761 ccttgtttat aaactgggca atcaagccct tcaccatgtt cgccatagcc tatttcttcc
     2821 tgggttattt gtttgtagat tatcttcccg gaactgagat tctgaaaaat ggtgaagaag
     2881 ttgaattatg gcgtagctat gttgccggtg caattctgct cggaattgct ccatgcactg
     2941 caatggtttt gatgtggggc cacctggcaa aagggaacgc cggcttaaca ctggtaatgg
     3001 tagcaataaa ttcactgata atgctcgtta tttttggacc tttaggagga ttgttattga
     3061 acataaattc catgcccgta ccatgggaaa ctatgctgtt ttcagtatca gtttatgttg
     3121 ctttcccgct gattgccggt tacctgtcaa ggaaatggat tcttcaatac aaaggtcaag
     3181 agtggttcaa tgaaaaattt ctgcattggc tcactccggt cagtataact gcattgcttg
     3241 tcactctgct tttactgttt tcatttaaag gagaagttat cgtcaataat ccgcttacta
     3301 ttatatggat ttctattcca cttttcattc agacagtttt gatttttgtg attggatatt
     3361 ttattttggc aaggtggatg aaattatcct atcacgatgc cgcgccggca gctttaattg
     3421 gtgcttcaaa tcattttgag gttgcaatag cgacggcagt tattttattt ggtttgtcat
     3481 cgggtgcagc actggcgaca gtagtaggag ttttgattga ggttcctctg atgctgatgc
     3541 tggtctcaat ttgtaaaaga acgtgtcatc tttttgatga gtgccacttg gactatccaa
     3601 actgcaaacc tgttcttgtg tctaatgaaa tatgactatc tcgtaaaaag tctgaaatac
     3661 acacagtcat ttctgtcgta aggagaagta ttactgatat tagaatggtc gcttatacaa
     3721 gatttctaac ttcactcgaa atgacaggtt tttagatatt tttacgactg catcaaggat
     3781 gaatattcta tttcatcaag acattcttta taaagacaaa atccaggaat aaggacaatt
     3841 atgcgaaagg tttacagttc tcctaaaacg aatttggtac attttgccaa gagcgttctg
     3901 gaagctaata atatagaaag tgtgatcctg agagaacagt tggctggagt cgtcggcggg
     3961 atagctcctc ttgatgcatg gcctgaactc tgggttcatg acgctgaaag gttggaagag
     4021 gctcgtcagt tacttgaggc ttcgatggcg aagtctgaaa cactggataa accctggcaa
     4081 tgtcagggtt gtggtgaaaa gattgaggcc cagtttaccc agtgctggaa gtgcggtaca
     4141 gagttctggt tatctgaaag ctgatttgat tacctaaatt gcaaaacgaa gcttaatacg
     4201 gttcggctgt tgcatatgtt atatttaggg tcagagtaan nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnt ggcacgtctt caaagagcgg caccaattgg tattcctgtg
     4381 catttattac aaagaggaaa caaccgtcag gattgcttta atatgaatgg agactatgca
     4441 gcatatttgt ggtggctcag ggagtattcc gaaaaattcc aggtagaaat ccacgtatgg
     4501 tctttaatga aaaatcatat ccatttgtta tgtactccac aattggaggg tgggctgagc
     4561 cgtatgatcc aggctttagg caggcaatac gtgcggtatt tcaattatca gaaccatcga
     4621 acaggtactc tttgggaagg gcgctacaga tcatgtttgg tccagcctga aggatatttg
     4681 cttaaagtct atcggtacat tgagctgaat tcggtgcgaa aaaaatatgt tagcaaaccc
     4741 tctgactacc attggtccag ttatcagata aacgcgcagg gaaagccttc cgcactttgt
     4801 aagccccacg ctgaatattt gaagctgggt gaatcaaaag cagaacggac agaaaaatat
     4861 caggaaatgt ttgaacaaag ttccagggaa gatttacttg aagaaatcag ggacagtaca
     4921 aacaaaggac tggcttttgg agatgagagt tttaagatag aggttgaaaa aatgacaggc
     4981 cgccgagtca gatcattgaa aagcgggaga ccgttaggtt ggcgaaaacc aaagtaatct
     5041 tgaaaaggtt tcaaaattat attttaaaaa ataacgtaaa taaatgaaaa aatatccaaa
     5101 aaaatctaaa gccgtctatt tttttggaac ctgcctggtg gacctgtttt atccggaggc
     5161 cggaatggca gggatcaagc ttctgcagcg tgaaggattg gaggtcattt ttccgcagca
     5221 gcaaacatgc tgcgggcagc cggccttcaa ttccgggttc catgacgaag ctcgtaaagt
     5281 tgccgcaaaa caagttgtct tatttccaaa agatattccg attgtgattc catccggctc
     5341 atgcggtgca atgatccgta agcattatgc tgaactattt aaaaatgatg acctcaaaac
     5401 tgaggcagaa tctgtggcag aacgtatttt tgagttaaca gaatttttag tatatgtact
     5461 aaaaataaaa cttaaagacc tgggtgaacc gattaaacta acgtggcata cttcctgtca
     5521 tgcaaagcgg gaaatgggaa ttgacactga accgaaggag ttattgcgtc agttgaaaaa
     5581 cgtcgaatta ctcgaacttg agcgggaaga tgagtgttgc ggatttggcg gtacatttgc
     5641 cgttaaggag cctcatattt ctgcggcaat ggtaaaagac aaggttgatg atatttcaaa
     5701 aactggtgca agtcgcttgc tcagtggaga ctgcggttgt ctgctgaata tttccggtgc
     5761 aatgaaacat tccggaattt cgacagatca tcagcatatt gcggaattca tttgggaacg
     5821 taccgcaaac aataaatctt aaaacaaaag tatacgatga atacagaaca catgagttta
     5881 gctgaagttg agagtctggc aactgacgca ttgatcggca caggaacacg tctggaatcc
     5941 gcgcagtcaa tgggtagggc tgcgcgagcg gctgaacgtg acggtatacg gagtcatgga
     6001 ctgctctacg ttccaacata ctgtgaacat gtgaagtgtg gaaaagtaga tggtactgca
     6061 gttcctgaaa taatccagcc tcgcttatcc gcaattaagg tggatgcaaa aacaggtttt
     6121 gcgcaccctg caattgaagc gggatttatt gaattgatac cgctcgttaa atcgaacggc
     6181 tgtgtcggac tcactataag caattcctac aactgtggag tactgggtta tcatgtggaa
     6241 cgccttgctg aagctggtct ggtaggaatc ggctttacga attcaccggc ctcgattgcg
     6301 cctgtcggag gaaaaaaacc tgtgattggt acaaatccgt ttgctcttgc agttccggat
     6361 tctgcgggag gtgtggcttt cgtgctggac cagagtgcaa gcgttgttgc caagagcgaa
     6421 attattatgc gtgcacggga aggagattcg atccctgaag gctgggcact cgatgctgag
     6481 ggtcaaacca cgacagatcc tgagattgct ctcaagggaa gtatggctcc aagcggaggc
     6541 tataaaggtt tcggtacagg tttgctggtc gaagttatgg ctgccgcact cagtggcgct
     6601 atgcttggtc ttcaggccag tcctttctcc ggcacagcag gcgggcctcc gagaacagga
     6661 cagtgtttcc ttgccttcga tccgaatgct tactctggag cagagtttgc tgagcgaatc
     6721 acaatcctga cagaagcaat ccagagtcag gagggtgcac gcttgccagg tgaccgtcgc
     6781 aaagagaatc gtcaacgtat tgagatcgaa ggtgttgagg ttgagaaatc cttgattaaa
     6841 cgaattcaga ctttttgtac ttgaaaaact cctaattagt taagagttca cagaaaaaaa
     6901 atcctttgtc attccagact tgatcaagga tcaagaaccc cgaacaagtg gattattccg
     6961 tctcaataaa ttactcttta cattcaagat gggcgcgagt gtacggtcca atctcttctt
     7021 cttctaaaac cttaaaatca ccgtaacagt atttaattgc ccgctcctga aactggttac
     7081 gatctcctgt tttgaaaata atgtattggg gttccctgct gcatccggtg tgcatctgca
     7141 gaaaaatcaa ggttgtccaa aggaacaata tattaaaaat tttctgctta tgaatcatta
     7201 ctttattttt ggttcaattg catgaacagg cttacactgt gccactctac aactgttcct
     7261 gcaacttcat ttcggaaagt ctcccgtaaa ttattaagtt gttccggaga aaaatatgct
     7321 gataaccgct gtccgtagct ggaatggggg ctgttctgat gagatggaaa ccattgttct
     7381 atttgttcag ttcgaatcat ggttgggatg cagtactctt taacctgcca gcgaatgata
     7441 ttccaatttg cacctttcag ctcatcacaa agagtggatg gtgtccagct gctgcgcaca
     7501 ttttcttcat ccaggtaaat tcctttttct gcggttttca actttttcct aaaatcgggc
     7561 tcaagcaatt tttccggaat caaatctgaa agacgctgcc ccagagcggg gaccgtctct
     7621 ccaaaaacga aaaagccatc agcagaaatc cacggtttta gagtttcaag aaaacccgat
     7681 ttgtcagcgt gtttttgcag tacattgcgg ccgacaatcc gctcaaaacg taaatctgca
     7741 atttcaaaag acgcaggaca tccggaactg tctactgcaa ttttagcttc aattccggaa
     7801 gtggtttttt tgaacaaagt gccaagtatt tttttttcgt tagaggattc aacaagtgca
     7861 taaacattct cctcagaaat acggcgaaga aattcaccaa ggagcaatcc ttcacctgca
     7921 ttgagcacca gtgcaagagt attgcgtgaa atctcgctcc attccgtcaa atgatcacga
     7981 aactcgttaa gcaattcccc tgaactgtca agtgttctgg cttcccaata aattgaacct
     8041 tcagattgtt cggatgcagt ttgggaaaaa aaagctgcaa gttgtgcttc gcggttccgt
     8101 gatacatttt cctgtataga agcttcaaaa ccttgttctg agggctgata aaaaatccgg
     8161 ccctgcaaaa catccggaag atactgctgt gcgacccaat gatcgcggta ggcatgtgga
     8221 taaagatagc cttctccatg tccaaaggct ttaccgtccc ggtttccatc tttgaggggg
     8281 tccggaactt catcagcctg ttcattccgc acaacagaaa ttgcatcaaa aaaggcaaaa
     8341 gccgaattac tttttggtgc ggtacttaaa tacaaacagg cttgtgccag gtgataacgg
     8401 ccttccggca tgccgacata atcaaaagcc tgggctgcag atgaaaccac acccaacgcc
     8461 tgcggatcag ccattccaac atcttctccg gcaaaaatca gcatcctccg gaaaatgaaa
     8521 cgcgggtctt cgcctgcttc caccattttt gccatccaga aaagtgcggc atccggatca
     8581 gaaccacgca tcgatttgat aaaagcggaa attgtgtcaa aatgcgcatc gccgtctttg
     8641 tcataaagca ctgcacgctg ctgaattgat tcttctgcaa cttccagtgt aatttgcagc
     8701 acgtcatttt cgtcagcttg acttgttaag acggcgagtt caagtgcatt caaggctgct
     8761 ctggcatcac ctccggcaac atgcgccagg tgcctgcgtg cctctgctgc tagatttact
     8821 tgcttgtctc caaaaccacg ttcagaatct gtcagtgcct gctcaattat ttcctccact
     8881 tcagttgatt tgagggactg aagttgaaaa attcgggagc ggctgacgag cgccttattg
     8941 acttcaaaat atggattttc ggttgtcgcg ccgatcagaa taactgtccc gttttccaca
     9001 tgcggaagga gtgcatcctg ttgcgctttg ttgaaacgat gtacttcatc aacaaacaat
     9061 accgcgtggc gctgatgttc tgtacgtact tttttggcag tttcaataca tttgcggata
     9121 tcagcgattc cagacaaaac ggcattgatt gaaagaaatt ctgccttggt agtattggca
     9181 attattcgtg caagtgtagt ttttcctgtt ccgggcggcc cataaaaaat caggcttgac
     9241 agttgatcgg cttgaatcgc acgccgtaaa aggcgtcctg gtgccaggat atgactttgt
     9301 ccgacaaatt cttcaattgt ccgcggacgc atccggtctg ctaaaggagc ctctttgcgg
     9361 atttgtttat cgtgtgcgtg ctcaaataaa ttcacttgaa aactattttc aattagaaat
     9421 gaatggaact ttagctgccg agtttatata gtgtgaggta catttcaagc tgtcattgaa
     9481 aaagattcta tcaacatttt ccataaacgg atactgcaaa aatatgcctt tcggttctac
     9541 aactcaagca cggattcgtg aacttagtac cctgattcat caatataatt attcgtacta
     9601 ctgcggaaat gattcagatg aaagcgaaaa atattgtgct gacgaaactt atgatgccct
     9661 cgtcttagag ctaaaagcat tggaaaatca gtatcctgaa ttgaaactgg aagattctcc
     9721 aaccttgcat attacaggtg aaccgcactc tgtttttccg agccgaaaac atctgattcc
     9781 aatgctcagt ttgggaaatg tttattcatt ggacgaactt aaaagctggg atacaagcat
     9841 tcaaaaactg cttggcggac aaaatacgga atacgtttgc gagttaaaaa tagatggggt
     9901 ggccatttcc gcaatttaca gaaacggatt attgagcgcg ggtgttaccc gtggtgacgg
     9961 cagcgaaggt gaggaaatta caccaaatat caaaacaatc agttgcctgc ctttgaagat
    10021 caatgatgaa cgggatttgg atgtacgggg tgaagtttat tttccacgga aaaattttga
    10081 cgcttacaat cagcaacgca tcagttccgg tgaactgcct tttaaaaatc caagaaacgc
    10141 agctgctggt tctttgcggc ttttggacag tactgaaaca cgacgcagaa acttagctgt
    10201 ctttatctac acaattgcag aaggtgcacc tgaggaatcc catgcaggaa atttggaatt
    10261 tttacaacaa caggaatttc cggtcaatcc ggaaacaaaa aaagttggat caatcgaaga
    10321 ggtgctcgaa ttttgtcgtt actgggaaga acataaagaa gaactgcctt atgatattga
    10381 cggcattgta ctgaaaatca attcacttaa acagcagcgg cagctgggtt ttactgcaaa
    10441 atcacctcgc tgggcgactg cttttaaatt tactgcagaa caggcttttt ctgtactgcg
    10501 ggaagtggaa gtcggggtgg ggcgtaccgg aaatttaaca cctgtggcaa ttcttgatcc
    10561 ggttgaactg aacggaacca tagtaggtcg cgcaacactt cataactatg atcaggtaga
    10621 acgattaaac ctgcatcttg gtgatcatgt gacactggaa aagggcggtg aaattattcc
    10681 aaaaattgtt gcagttgatc caactaagcg ccactcagct gcgcaaaaaa tcaagccgcc
    10741 gcaatgctgt ccttcgtgtg gtacttcagc tgttcgtttt caagatgaag tggagtggcg
    10801 ctgtccaaat tctgaatgtc ctgctcagca aaaagagcag attttacatt ttgtttcacg
    10861 cagagcaatg gatatggaca caattggccc cgcattgatt gaacaattga tgactaaaaa
    10921 tttgctgcaa aatgccgcgg acctttattt gcttcgtcac gaagacttgt caggactcga
    10981 acgtatggct gataaatctg cagcaaatgt actgtccagt atagaaaaat cgaaaaagtg
    11041 tgagttggct caattcgttc atgcacttgg aattgcaaat gttggagaaa aaacagctag
    11101 catcttggca cagcattttg gtactctgga aagactaatg tctggtacgc cggatgattt
    11161 tgaaaaagta gaggccattg gtccagtgat tgctgaaagt ataattgatt tttttcagga
    11221 tcagcggcaa cgtcagctaa ttgctgattt tctcgaacgc ggtgtttctc ctgctgaggt
    11281 aaaaattctg gagattgttg actcgccatt cagcggaaaa attgttgttt tgaccggaac
    11341 gctttctgag ccccgcgatg tttggaaaaa acgcctgatc caggctggag caaacgtaac
    11401 cggcagtgtt tccaaaaaaa ccgactttgt gcttgccggt gaaaatgcag gttcaaagtt
    11461 ggaaaaagca gaaaaacttg aggttgcagt gattgacgaa acaacggcac ttaatctgtt
    11521 ggaacaaatc agttgatttc tcattattga tacaatagta aaaagtttga aatccatact
    11581 gtcatttcga gcgaagcgag aaatcttaat gatattagtg tggtcgctta tacaagattt
    11641 atcgcttcgc tctaaatgac aaggtttcag gacttttttt tagattgaat cactttcaaa
    11701 aatacggttt gaactgatag ctgacaactg acaactgata gctgattgca gaaaacttaa
    11761 aactgattac taaaaactac ccatgaaagg tgtaatttta gctgctggtt atgcaaccag
    11821 atttttacct gcatcaaaaa caattcccaa agaaatgttt ccgcttattg accgtccggc
    11881 cattgatttc attgtgcagg aaatggtgga ttcaggtatt cacgatattt tgctagtttc
    11941 gtcacgcagg aagaaagtga tggaggatta ttttgatcgg gagattgagt tgtcttctgc
    12001 attttcgaag tccaacgaaa ttgaaaaact ggaagtaata aaaccaatcg aagccaacat
    12061 ttttacattg cgccaacaac gaatgatggg cacagggaac gccttgatgc tggttgaacc
    12121 gtttgtagat aatcagccat ttgtcgtggc ctatcctgac gatatagttc ttggtgaaaa
    12181 gcctctcagc aggcaactga ttgatgcctg ggaaaaaacc ggcaacacgg ttttggcagt
    12241 taaagagttg gaggaagatc agctttctcg ctatggagtg attgacccgg cagattccgg
    12301 aaaaattatg caggtcaata aaatggtaga aaaacctctg ccgggtactg cacccagtcg
    12361 ttttgtttcc tatggacgtt acctctacac tccggatctt tttgaggcac tgcgcaggag
    12421 cgataaaagt cattcgcatc agagtgaatt tactcaaacc gaggcgatca atcatctggc
    12481 tgcacagggg caggtgtcgg ccctgcagtt tgaaggaacc cgttatgatt tgggagaacc
    12541 actcggtttt ctgacttctg ccatgcaaat cggcttgttg cgtccggaat acaagactgc
    12601 tttgctggat gaaatgcgga ggttgctcaa actgcacgac aatcccgaca atgaaaacag
    12661 cggttaaagt actcttcttt ttatcccgtt gctgttttta tctctccagt ttaataa
//
LOCUS       DTVD01000013           12671 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1120_length_12671_cov_296.222812, whole genome
            shotgun sequence.
ACCESSION   DTVD01000013 DTVD01000000
VERSION     DTVD01000013.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12671)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12671
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_12671_cov_296.222812"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="EYO68_00430"
     CDS             complement(<1..615)
                     /locus_tag="EYO68_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06303.1"
                     /translation="MNPLFRSGMIVLILLFTWISSAHALIFERRKTYDSEISWFVYPV
                     IGSIPGVQDFYGLGGTVSGIGGSESDITAVSLRGKAKYFDDDFQIDILSIFDIPLFTE
                     HLTFTWFSTKIRNVGWPEGQRGIDSDPDSMYYLLATSVEASGGELSVNILDNQLEAYY
                     AYSDAAVKPYGLVDPNGTFYNAQNAGIIESPRGYRYGLYLDDTDN"
     gene            complement(636..1829)
                     /locus_tag="EYO68_00435"
     CDS             complement(636..1829)
                     /locus_tag="EYO68_00435"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIC06304.1"
                     /translation="MSKIKGTIAILTGGGDVPGLNPAIRAATIRANRNGYRVVGLRNG
                     WEGIMKIDRTKSLENQQYCQILTEQNVQKIARTGGTFLHTSRTKPSKVAKEKVPESLQ
                     EQYNADENDLTAEIIKNLEYLGIEYLVPIGGDDTLSYGLRLHEAGVRCVGIPKTMDND
                     VPGTDYCIGFSTCVTRTIEFSHQLRTCAGSHERLLVIEVMGRYAGYTALVPAMAGASD
                     RCVIPEYEFDIERLTELLVKDRRDNESNYSVVLISEGAMFKGAEMIFSNQETDAYGHK
                     KLGGIGDMVSAELVARAPKYNKGESIQTINQRMSYMTRSGQPDAIDSIVPMVYGNLAM
                     DLIMEGKSGRMVCLKNGRYDHVPFEVVTEYDKKIDVDRFYDTKRYRPHYHNCMGMPQF
                     IMTSD"
     gene            complement(1839..3344)
                     /locus_tag="EYO68_00440"
     CDS             complement(1839..3344)
                     /locus_tag="EYO68_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIC06305.1"
                     /translation="MSLFQKEPKLPERLSIIFAASEVEGFSKSGGLADVARALPLHLK
                     SMGHDVRIVTPLYRLIPENHKTETIIPALGVPLGSEESWCAVRQLEMEGVVDGKMISV
                     PVYFIEHDHYFFRSGYYDDGLHEYPDNAARFGFFSKAVLQLCRALEWFPEIFHANDWH
                     TALLPFYLRQSQQETDEFKQSGSVLTIHNAGYQGNAPGTFRNSLEIPLDYFVAELFED
                     HNQLNLLKGGIYFADQVNTVSPGYAEELRTQLGSHGLHEIYTRKGENFSGILNGCDYD
                     EWNPETDPHLPACFSATDLKGKKQCKKKLQEIVSLPELPDVPVIGMVSRLTWQKGFEY
                     LLPALPQILSLEVQVIVLGSGEPLIANAFDKLQQKFPTKLGWYNGYNNELAHLIEAGS
                     DMFLMPSLYEPCGLNQMYSLRYGTLPIVRRVGGLKDSVIQFDEKLETGNGFIFDDPDS
                     DSLSEEVELAVSVFYNNPRRFKKMMDNAMKQRFAWKTAAEEYVALYRKALN"
     gene            complement(3310..4539)
                     /gene="glgC"
                     /locus_tag="EYO68_00445"
     CDS             complement(3310..4539)
                     /gene="glgC"
                     /locus_tag="EYO68_00445"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIC06306.1"
                     /translation="MSNVLTMILAGGEGARLKPLTAVRTKPAVPFGGNYRIIDFVLSN
                     FVNSGLLKIFVLTQFKSHSLMQHLREGWRFSSGIVKQFIDPVPAQMRTGKKWYEGTAD
                     AIFQNMNLIDDEDPDQVCIFGGDHIYRMDIRQMLKFHSKRQAVLTVAAIPVPVKDAHH
                     FGIIEVDEEWRMVSFEEKPKRNPKTIPGNSDYVLASMGNYIFERTPLVKELKRDAADK
                     KSSHDFGNDILPRMFPNGKVFVYDFSQNYIPGAEEEEKGYWKDVGTLDTFWEANMDLV
                     GLHPHFNLYNKKWPVLTYIPPLPPAKFVHFNDSRTGHAINSMIGSGTIISGALVENSV
                     VGYSVRIHSFAHVEHSVIMNNVEIGREAKIRKAIIDKHVRIAPGANVGYDLDTDRKKF
                     QITENGIVVIPKGAKVA"
     gene            4665..4847
                     /locus_tag="EYO68_00450"
     CDS             4665..4847
                     /locus_tag="EYO68_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06307.1"
                     /translation="MKKEIPVKQHGAARIPSPLPLSSNSKHEEFHFDNDDARILHDAT
                     ECSTGNVKKQISFEKS"
     gene            4980..7280
                     /locus_tag="EYO68_00455"
     CDS             4980..7280
                     /locus_tag="EYO68_00455"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00571.26,HMM:PF00654.18,HMM:PF02080.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIC06308.1"
                     /translation="MKFSKKLLNQCQEFVKSNLSEWRMLDADSSVMLVTSLIVGIGSG
                     LGAVLFRRLIEWFQSLAYRDISGLLTEWYPLHLILIPALGGAIVGPLVYYFAREAKGH
                     GVPEVMEALELRGGKIRPRVVIVKSLASSVCIASGGSVGREGPIAQIGSALGSFVGQY
                     LKLSEDRVRTLVACGAAGGIAATFNAPIAGAVFALEVLLRRFGSVYFGAVVISAVTAD
                     VIAHYFEGDQRTFLTPEYSLNSPWELLLYTLMGFIAALASVGFSRMLYFSEDIWNLIR
                     IPEPTKPIFGGVLLGLLGIASYQIDGFPRVFGVGYETIESTLFSQLTLQVTFGLLILK
                     LIATTLTLGSGGSGGIFAPSLFMGAMLGASFGQIAHTLFPEIVAPSGAYALVGMAAFF
                     SGAAHAPITAILILYEMTGDYQIILPLMLATVISTIVSRNLSHDSIYTLKLKRRGVEL
                     SEDTQAIDLMQGVTAQIAMNSETNAVPFDMPLVELMNAFSSTHYHALPVVKDETKLIG
                     VITIKELDQLRSGGLLESKTIAEILTNKKPATILPHQPVWMALRHLEDQGEGCVPVIS
                     ETSENTLLGVLRRIDIIRAYNKAVSQRAQDQHHDEILNIRKLNRTGLTEVTLGPKSPI
                     VGKRVKELRLGDDTLMVSVRRKGKLRIVRGETILHANDKVTIFSEKPKADFLENYLNG
                     TLDDSELPEESLVCNREVEIPPESSIDGKRIRDLSLPEDCVLVKIIRNRQIILPRGDT
                     VLYSGDIVEIFGVDEKLLEAESNLVS"
     gene            7474..9717
                     /locus_tag="EYO68_00460"
     CDS             7474..9717
                     /locus_tag="EYO68_00460"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIC06309.1"
                     /translation="MTTSPLKIIWTKTDEAPMLASYSLLPIVQAFTQDTGISIESRDI
                     SLSGRILSSFPDKLTENQKVPDELMILGELTQDPEANIIKLPNISASIPQLKAAIAEL
                     QSHGFNIPDYPESPQNETETNIKNRYAKVLGSAVNPVLREGNSDRRAATAVKNYARRH
                     PHSMGAWSADSKSHVATMNGGDFFANEKSTTIKESTSAKIEFVAADGKSTVLKDNLSL
                     EAAEIVDATFMSNKALDTFLDQQIQDAKKQGVLFSLHLKATMMKVSDPVIFGHCVKVF
                     YATVLEKHVETIADLGVNFNNGIGDLYSKMEEDKKISPDKKTEIKADIQALYADRPDL
                     AMVDSDLGITNLNVPSDVIIDASIPAAIRTSGKMWGPDGELHDTKFIIPDSSYAPLYA
                     ATFENCIANGALDPATMGTVQNVGLMAKKAEEYGSHPTTFEAPGNGVIRIVDSEGETI
                     HEHNVEQGDIWRMATVKDAPIKDWIKLAVTRAKITGWPAVFWLDEKRAHGREMIKKVK
                     SYLKIHDTQNLELPIMSVYDAAKYSIERARSGKNTISVTGNVLRDFNTDLYPILELGT
                     SAKMLSIVPLMKGGGLFETGAGGSAPKHVQQFVKESHLRWDSLGEFLAMAESLIHLAR
                     TTDNTKAAVLAKTLNQANEHFLTHNKSPSRKVHELDNRGSHFYLAMFWAQALAGQTED
                     TEIQSRFSGLAKELEEKENIIIEELNSAQGKELDLGGYYDPDEKKVTEAMRPSATLNA
                     LFSAFCG"
     gene            9980..10963
                     /locus_tag="EYO68_00465"
     CDS             9980..10963
                     /locus_tag="EYO68_00465"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIC06310.1"
                     /translation="MKTVKVAVTGAAGQIGYAMLFRLASGEIFGRDTAVELQLLELEQ
                     ALPALEGVKMELDDCAFPLLTNIVTTSDPNVAFKDIDWALLVGSVPRKAGMERNDLLR
                     INGGIFVGQGKALNDNAGQNARVLVVGNPCNTNCLIAMNNAPNISRNNWFAMTALDEN
                     RAKSQLAQKAGAAVRDVSNMTIWGNHSATQYPDFYNAKIQGKDAPAVINDEEWLQGDF
                     IKTVQQRGAAIINARGASSAASAANAALDTILRISTPTSEGDWFSTAIPSDGSYGIPE
                     GLIFSYPLRSKGDGDYEIVQGIELNEFSQEKIRATRDELEMEREAVKEMLS"
     gene            10976..12151
                     /gene="sucC"
                     /locus_tag="EYO68_00470"
     CDS             10976..12151
                     /gene="sucC"
                     /locus_tag="EYO68_00470"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIC06311.1"
                     /translation="MKIHEYQAKQILAKYGVPVPRGSVAYSVDEATKVAEDLGTDVCV
                     VKAQIHAGGRGKGGGVKVSKGMDAVRENAEAILGMQLVTHQTGPQGQKVKQLLVEEGM
                     DIRKELYCSVLVDRGKQRIVLLASTEGGMDIEEVAEKTPEKILKVFVDPAVGLRSYQA
                     NELAFGLKIDKINPKLIRPAAAVFKALYQAFVQEDCSLVEINPLVLTGDGRVVALDAK
                     LSFDDNAMFRHKDNLAFRDKDEEDPLEVEASEADLNYIKLDGSIGCMVNGAGLAMGTM
                     DIIKAYGGDPANFLDVGGAASQENVEKAFRIITMDPNVKCILINIFGGIVRCDMVAAG
                     VIAAFKSVDLQIPVVVRLEGTNSEEAHILVSKSGLGERLQMADGIREAAKKAVAAVS"
     gene            12315..>12671
                     /gene="ssrA"
                     /locus_tag="EYO68_00475"
     tmRNA           12315..>12671
                     /gene="ssrA"
                     /locus_tag="EYO68_00475"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3619 a         2770 c         2763 g         3519 t
ORIGIN      
        1 attatccgta tcatccagat acagtccata gcggtagccc cttggacttt ctataattcc
       61 tgcattttgt gcattgtaaa atgtgccgtt tgggtcgact aatccgtatg gtttgactgc
      121 agcatcactg taagcgtaat atgcctccaa ctgattatct aaaatattta cagaaagttc
      181 tccaccggaa gcttctacac tggtggccag cagataatac atactgtccg ggtcagaatc
      241 tattccacgc tgaccttccg gccagccgac gttacggatt tttgttgaaa accaggtgaa
      301 ggtaagatgt tctgtaaaaa gtggaatatc gaaaattgaa agaatatcta tctgaaaatc
      361 atcatcaaaa tatttcgctt tacctctgag actgactgca gtaatatcag actcgcttcc
      421 tccaattcct gaaactgtac ccccaagtcc ataaaaatcc tgtactccgg ggatactacc
      481 tattacagga taaacaaacc aggaaatctc agaatcataa gttttgcgtc tctcaaaaat
      541 taaagcatga gccgaggata tccaggtaaa caaaagaatc aggacaatca taccgctgcg
      601 aaacagagga ttcatttagt tacaattagt aattttcaat ctgaagtcat aataaactga
      661 ggcatgccca tgcagttatg ataatgtggc ctgtatcgtt ttgtgtcata aaaccgatcc
      721 acatcaattt ttttatcata ttcagtcacc acctcaaatg gtacatgatc gtacctgcca
      781 ttttttaagc aaaccattct accgcttttt ccttccatta tcagatccat cgccaggtta
      841 ccgtaaacca tcggtacaat ggagtcaatt gcatcaggct gtccggagcg agtcatgtaa
      901 ctcattcttt gatttatcgt ttgaatcgat tctcctttgt tgtattttgg agcgcgtgcc
      961 accagttctg cactgaccat atcacctatt ccaccaagtt ttttgtgacc ataggcatca
     1021 gtttcctgat ttgaaaaaat catttccgca cctttaaaca tggcaccttc tgaaatcaat
     1081 acaacggaat agttgctttc attatcccgg cggtctttaa ccaatagttc tgtcaggcgt
     1141 tctatatcaa attcatattc cggaatcacg caacggtctg atgctcccgc cattgccggt
     1201 accaaagccg tatatcccgc ataacgtccc atcacttcaa tcaccagcag cctttcatgt
     1261 gaaccggcac aggtacgcaa ctggtgagag aattcaatag tacgggttac gcaggtgctg
     1321 aaaccgatgc aataatctgt acctggaaca tcattatcca tcgttttcgg tattccaaca
     1381 caacggacac cagcctcatg cagccgcaga ccgtaactga gggtatcatc acctccaatc
     1441 ggaaccagat attcgatccc caggtattcc agatttttga tgatttctgc agtcaaatca
     1501 ttttcatccg cattatattg ttcctgcaat gactccggca ctttttcttt ggcaacttta
     1561 ctgggtttgg tgcgggatgt gtgcagaaaa gttccgccgg ttcgtgcgat tttttgtacg
     1621 ttttgttctg tcaggatctg gcaatactgc tgattttcca gggactttgt tcggtcaatt
     1681 ttcatgatac cttcccagcc gttcctgaga ccaacaactc tgtaaccatt tcgattggca
     1741 cggatagttg ccgcacgaat agccggattt agtcccggta catctccgcc gcccgtcagt
     1801 attgcaatag tgcctttaat ttttgacata ttcctccatt aatttaatgc ttttcgataa
     1861 agagccacat attcttctgc agccgttttc caggcaaatc gctgtttcat ggcattgtcc
     1921 atcatttttt tgaatcgcct tggattattg tagaaaacac tcacagccaa ttcaacctct
     1981 tcacttaaag agtctgaatc cggatcatca aaaatgaatc cgttaccagt ttccagtttt
     2041 tcgtcaaact gaataacgga gtctttcaaa ccaccgactc ttcttacaat cggaagagta
     2101 ccataacgca agctgtacat ttgattcaat ccacatggtt catacagcga gggcatcaga
     2161 aacatatcac tgcctgcttc aatcaagtgt gccaattcat tattgtaacc attataccag
     2221 ccaagttttg tcggaaactt ttgctgcaac ttgtcaaaag catttgcaat caaaggctcg
     2281 ccacttccca gaactatcac ctgcacttcc aaacttaaaa tttgcggcaa agcaggaagc
     2341 agatattcaa aacctttttg ccaggttaag cggctgacca tgccaattac aggaacatcc
     2401 ggcaactcag gcagactaac aatctcctgc agtttttttt tgcactgctt tttgcccttt
     2461 aaatcagttg cactaaaaca tgcaggcaaa tgagggtctg tttccggatt ccattcatca
     2521 taatcacaac cgttcagaat tccggaaaag ttttcacctt ttctagtgta tatttcgtgc
     2581 agaccgtgac tgccaagttg agttctcaat tcttccgcat atcccggact gaccgtgttt
     2641 acctgatcgg caaaataaat acctcctttg agtaaattca attgattgtg gtcttcaaac
     2701 aattccgcaa caaaataatc caggggaatt tcaagggagt tcctgaaagt tccgggagca
     2761 ttgccctggt atccggcatt gtggatggtc agaacagaac cactttgttt gaattcgtca
     2821 gtttcctgct gagactgtct tagataaaac ggcaaaagag cagtgtgcca gtcgtttgca
     2881 tgaaaaattt ccggaaacca ttccaatgct ctgcaaagct gcaaaactgc ttttgagaaa
     2941 aaaccaaacc gtgcagcatt gtccggatat tcgtgcagac cgtcatcgta atatccactt
     3001 ctgaaaaagt agtgatcgtg ttcaataaaa taaactggaa cacttatcat tttcccgtct
     3061 acaacacctt ccatttcaag ttgacgtact gcacaccaac tttcttcgct tcccagcggt
     3121 acaccaagcg caggaatgat cgtttcagtt ttatgatttt cggggattaa gcggtaaagc
     3181 ggcgtaacta tccggacatc atgtcccatg gattttaaat gcaaaggcaa cgcccgtgct
     3241 acatcagcaa gtcctccgct tttggaaaaa ccttcaactt cagatgccgc aaaaataata
     3301 ctgagccgtt caggcaactt tggctccttt tggaataacg acaattccgt tttcggttat
     3361 ctgaaatttt tttctgtctg tatcaagatc atacccgaca tttgcaccag gtgctatgcg
     3421 tacatgtttg tctatgatgg cctttcttat ttttgcctct cgtccaattt cgacattgtt
     3481 catgataaca gagtgttcaa catgagcaaa actgtgaatt cggacactgt agcctaccac
     3541 cgaattttct acaagagccc cgctgatgat agtacctgag ccaatcatcg aattaatagc
     3601 atgtcctgta cgagagtcat tgaaatggac aaactttgcc ggaggaagag gtggaatata
     3661 agtcagcaca ggccattttt tattgtagag attaaaatga ggatgtaagc ctaccaaatc
     3721 catattggct tcccaaaatg tatcgagtgt gccgacatcc ttccagtaac ctttttcctc
     3781 ttcctctgct ccgggtatat aattttggct gaagtcgtaa acaaatactt ttccattagg
     3841 aaacattctg ggcaaaatat cattgccaaa atcatgactt gattttttat cagctgcatc
     3901 cctcttcagt tctttgacca gaggagttcg ttcaaaaatg tagttgccca ttgaggccag
     3961 cacataatca gaattccccg gaatcgtttt tggatttctt tttggttttt cctcaaaact
     4021 aaccatccgc cattcttcat ccacttcaat aataccgaaa tgatgtgcat ctttaacagg
     4081 cacgggaata gccgccaccg taagaaccgc ctgacgcttg ctgtgaaatt ttaacatctg
     4141 tcggatatcc atacggtaaa tatgatctcc tccaaaaatg caaacctggt ccggatcttc
     4201 atcatcaatc aagttcatgt tttgaaaaat agcgtctgca gttccttcat accatttttt
     4261 cccggtccgc atttgtgccg gaacaggatc gatgaactgt tttacaattc cagatgaaaa
     4321 acgccagcct tcacgcaaat gctgcatgag cgaatgtgac ttaaactggg tgaggacaaa
     4381 tattttcaat aaacctgagt ttacaaaatt acttagcaca aaatcgataa ttctgtaatt
     4441 tccgccaaaa ggaactgcgg gcttggttct cactgctgtc aagggtttca gacgggcgcc
     4501 ttcacctcca gccagtatca tagtcaatac attgctcatg attctccttt tctatctttg
     4561 caaatgtgcc gctttgtgat atttttccaa acacacatca gttttgcagg gcagccgaat
     4621 ctgaatacaa tactctcatt atttttgttt tgtaacaagc aaatatgaaa aaggaaattc
     4681 cagtaaaaca acatggtgcc gcccgcatcc cctctccatt accgcttagc agcaattcaa
     4741 aacacgaaga attccatttt gataatgacg atgcgcgcat tctccacgat gcaactgaat
     4801 gttcaacagg caacgtaaaa aaacaaattt cttttgaaaa gtcataaccg catacaagct
     4861 gtttttttga tatatcaaaa gtctgggctg taatattatg aaaagtattc accaaaaaac
     4921 tagttcggtt tggacttaat taagaatcta aatttttttc agatttagaa caatacagta
     4981 tgaaattttc aaaaaaatta ctgaaccaat gtcaagaatt tgttaaatca aatctctcag
     5041 aatggcgcat gcttgatgca gattcatcag tcatgcttgt cacatctcta atcgtcggaa
     5101 tcggttccgg attgggagca gttcttttcc gtcgacttat tgaatggttt cagagtttag
     5161 cctacagaga tatttccgga ttactgacag aatggtatcc actgcatttg atattgatcc
     5221 cagctctggg cggagcaata gtggggcctc tggtctatta ctttgcccgt gaggcaaagg
     5281 ggcatggcgt cccggaagta atggaagctc ttgaactgcg tggtggcaag attcgtcctc
     5341 gtgtagtgat tgttaaatct ctggcgtctt cagtttgtat agcaagcgga ggctcggttg
     5401 gacgcgaggg gccaatcgct caaatcggtt cagctttggg ttcattcgtt ggacagtatt
     5461 taaaactttc tgaagatcgc gtgcgcaccc ttgttgcctg cggtgctgcc ggaggaattg
     5521 cggcaacttt taatgcgccg attgccggag cagtgtttgc gcttgaagtt cttttgcgtc
     5581 gtttcggcag cgtttatttt ggagccgtcg ttatttctgc ggttactgca gatgtaattg
     5641 cacattactt tgagggagat cagcgcactt ttttaactcc ggaatattca ttaaacagtc
     5701 cctgggaact gttgctttac actttgatgg gttttattgc agcactggct tcagttggat
     5761 ttagtcgaat gctctatttt tcggaagata tctggaactt aatccgtatt ccggaaccaa
     5821 caaaacctat atttggagga gtactgctgg gcttgctggg aatagcctcc tatcaaatcg
     5881 acggtttccc gcgtgttttt ggcgtcggct atgaaactat tgaaagcacg ctattcagtc
     5941 aactgactct ccaagttact ttcggtctgc tgattttgaa acttattgcc acaaccctga
     6001 ctctcggttc cggaggttca ggcgggatat ttgcgccctc attgtttatg ggtgccatgc
     6061 ttggagcttc ttttggtcaa atcgctcaca cattatttcc tgaaattgtg gctccgtccg
     6121 gtgcttatgc attggttgga atggcagcgt tttttagcgg cgctgcgcat gctccgataa
     6181 ctgcgattct cattctttat gaaatgactg gcgattacca aataattctg cccctgatgc
     6241 ttgctactgt aatcagtaca attgtttcca ggaatctcag tcatgattca atttatacac
     6301 taaaattgaa gagacgcggt gtagaactgt cggaggatac tcaggcaatt gacctgatgc
     6361 agggtgtaac agcacaaatt gcgatgaact ctgaaaccaa tgctgtacct tttgacatgc
     6421 ctttggttga gttaatgaat gctttttcca gcacacatta tcacgccctg ccggtggtga
     6481 aagatgaaac caaattgatt ggagtgatta ctattaaaga actggatcag cttcgatccg
     6541 gaggtttact tgaatcaaaa accattgctg aaattttgac caacaaaaaa ccggcaacaa
     6601 tacttccaca tcagccggtt tggatggctt tacggcatct ggaagaccag ggcgaaggct
     6661 gcgttcctgt catttctgaa acaagtgaaa acacattact gggggtactg aggagaatcg
     6721 acatcattcg ggcatataac aaagcagtat cccaaagggc tcaggatcaa catcacgatg
     6781 aaattctgaa cattcgaaaa ctaaaccgga caggactgac tgaagttact ttaggcccga
     6841 aatcgccgat tgttggaaag cgggtaaaag aattacgtct tggagacgac acgctgatgg
     6901 tttccgtacg ccgaaaagga aagttgcgca ttgtacgggg tgaaactatt ttacatgcca
     6961 atgataaagt tactatcttt tccgaaaagc ccaaagcaga ctttctggaa aattatctta
     7021 atggaacttt ggatgattca gagctcccgg aagagtcact cgtctgtaac cgtgaagtag
     7081 aaatacctcc ggaatcctcc attgacggaa aacgaatccg agatttgagt ctaccggaag
     7141 attgtgtgtt agtaaaaatt attcgtaacc gtcaaattat cctccctcga ggcgatactg
     7201 ttctgtacag cggagatatt gttgaaatat ttggagttga tgaaaaactc ctggaggccg
     7261 aaagtaattt ggtcagctaa ttcgctgcag caacaccaaa ataccttttt agaattgaaa
     7321 taattgacca atctggtaga ctggattgtg atgtttttga tttccgcata tttggaaatc
     7381 tcttatatcg gggagccaaa atccgaacat tggtggtttt ctgcctctca tttcggacaa
     7441 tgtaatccaa tgtttaggac agtctcaaaa taaatgacaa cttcaccctt aaaaattatc
     7501 tggaccaaaa cagacgaagc tcccatgctc gccagttatt ctttacttcc cattgtacag
     7561 gcatttactc aagacacagg aatttccatt gaaagccgtg atatttcact ttcaggacgc
     7621 attctttcct cctttccgga taagctgact gaaaaccaga aagttccgga cgaactcatg
     7681 atactcggcg agttgactca agaccctgaa gctaatatca tcaaacttcc aaatatcagt
     7741 gcctcgattc cgcagttaaa agcagcaatt gcagaactcc aatcacacgg attcaatatt
     7801 ccagactatc cggaaagccc gcaaaacgag actgaaacca atatcaaaaa tcgctatgct
     7861 aaagttttgg gaagtgcagt caatccggtg cttcgtgaag gtaattctga tcgccgtgct
     7921 gcgactgccg taaaaaatta tgccaggaga catccccact caatgggtgc atggagtgcg
     7981 gattcaaagt ctcatgtagc gacaatgaat ggtggagatt tttttgcgaa tgaaaaatcc
     8041 acaaccatca aggaatcaac ctcggctaaa atcgaatttg tagcagcgga cggaaaaagt
     8101 actgtattga aagacaacct gtcgctggag gccgcggaaa tcgtggatgc aactttcatg
     8161 agcaacaaag ctctggatac gttcctcgat cagcaaatcc aagatgccaa aaagcaggga
     8221 gttctttttt ctctgcacct gaaagcgacg atgatgaaag tctctgatcc ggttattttt
     8281 ggacactgcg ttaaggtttt ttatgccaca gtacttgaaa aacacgtaga gaccattgcc
     8341 gatttaggcg tcaatttcaa caacggcatc ggcgaccttt attcaaaaat ggaggaagat
     8401 aagaaaatat ctccagataa aaaaactgaa attaaagcag acattcaagc tctttatgcg
     8461 gatcgacccg atttggcgat ggttgattct gatctcggga ttacgaattt gaatgttccc
     8521 agtgatgtta ttatcgatgc ctcaattcct gccgcaatcc ggacttccgg taaaatgtgg
     8581 ggtcctgacg gtgaactgca cgataccaaa ttcattattc cggattccag ctacgccccg
     8641 ctttatgcag ccaccttcga gaattgtatc gccaacggtg cattggatcc ggccacaatg
     8701 ggtactgtac agaatgttgg gttaatggcc aaaaaggcgg aagaatatgg ttcacacccg
     8761 accacttttg aggcgcccgg aaatggagtc atacgtatcg tcgactccga gggagagaca
     8821 atccatgaac ataacgtgga acaaggggat atttggcgca tggctactgt caaggatgct
     8881 ccgatcaagg actggatcaa attggctgtc actcgtgcaa aaattacggg atggcctgct
     8941 gttttctggt tggacgagaa gcgggctcac ggacgggaga tgataaagaa agtcaaatcc
     9001 tatctcaaaa ttcatgacac tcagaattta gagcttccga taatgtcggt ctatgatgcc
     9061 gcaaaatatt ccattgaacg tgcacgttca gggaaaaaca ctatttcggt gacaggaaac
     9121 gttctccgtg atttcaacac tgatctctat cctatactcg aacttggcac tagcgccaag
     9181 atgctctcaa tcgtaccatt gatgaaaggc gggggcttgt ttgaaacagg agctggcggt
     9241 tctgcaccaa aacacgtgca gcaatttgta aaggagtcgc atttacgttg ggattctctg
     9301 ggagaatttc tggcaatggc agaatcattg attcaccttg cacgaactac ggacaacacc
     9361 aaggccgccg ttttggcaaa aactctaaat caggccaatg aacattttct gacacacaac
     9421 aaatccccct cgcgtaaagt ccacgaattg gacaatcgag gcagccactt ctaccttgca
     9481 atgttttggg cacaagctct ggcaggccaa acggaagaca ctgaaattca aagccgcttt
     9541 tccggactgg caaaagagct tgaggaaaag gagaatataa ttattgaaga actgaactcc
     9601 gctcaaggca aggaactcga tttgggagga tactatgacc ctgatgagaa aaaagtcact
     9661 gaagctatga gacccagcgc tactttgaat gcgttgtttt ccgcattctg tggctgaata
     9721 ataatgatgg agtgatctgg gaaagctccg tcttaatcta aaataatgaa acccaaattt
     9781 aataattttc gcagtttgtt tgcaaaaaac aaatttagca taaagttact gtgcttacga
     9841 aaatccctag tgcttagtgc ctcagtgcct tagtgtctca gtaccttgat gcctcagtgc
     9901 ctcgctgtca ttgtgtggta ttttagtgaa aaagtaaaaa tacgaattat ttgtttaata
     9961 cctaaaaaaa ggagacccca tgaaaactgt taaagttgct gttaccggcg cagccggaca
    10021 aattggatat gctatgctgt tccggttggc ttccggagaa attttcggca gggacactgc
    10081 agtcgagttg cagttgctgg aactcgaaca agctttacct gcactggaag gtgtaaaaat
    10141 ggaactcgac gattgtgctt ttcccttgct gacgaatatc gtcacgactt ccgatccaaa
    10201 tgttgctttc aaagacatcg actgggcact actggttggt tctgttcctc gaaaagcagg
    10261 tatggaacgc aatgatttac tgcggattaa cggaggaatt tttgttggac agggcaaagc
    10321 tctcaatgac aacgccggcc aaaatgcccg tgttttggtg gttggtaatc catgcaatac
    10381 taattgcctg attgccatga ataacgcacc gaatatttcc agaaataatt ggtttgcaat
    10441 gaccgctttg gacgaaaatc gagcgaaaag tcagcttgcg caaaaggccg gagctgcggt
    10501 gcgggatgtg agcaacatga ctatctgggg caatcacagt gccacacaat atcccgattt
    10561 ctacaacgct aaaatccagg gcaaagacgc tccggctgtg attaacgatg aagaatggct
    10621 gcagggagat ttcattaaaa cagtacaaca gcgcggagcc gccattatca acgctcgcgg
    10681 agcttcttcg gcagcatctg ccgcaaatgc ggcactggat acaatcttgc gaatcagcac
    10741 accaacctcg gaaggtgatt ggttcagcac ggcgattccg agcgatggaa gctatggaat
    10801 tccggaaggg ttaattttca gttatccact cagaagcaaa ggcgatggtg actatgaaat
    10861 tgttcagggc atcgaattga atgaattttc tcaggaaaaa atccgggcta cacgtgatga
    10921 actggaaatg gagcgtgagg cagttaagga aatgcttagc taaataaagg aagcaatgaa
    10981 aatacatgaa tatcaggcca aacaaattct ggcaaaatat ggcgttccgg ttcctcgcgg
    11041 aagtgtggct tattctgtag atgaggcaac taaagttgcg gaggatttgg gaactgacgt
    11101 ttgtgtggta aaagctcaaa tccatgcagg tggtcgtgga aaaggcggag gagtcaaagt
    11161 ttccaaggga atggatgccg tcagggaaaa tgctgaggca attctcggta tgcagttggt
    11221 aacacaccag acaggcccgc agggtcagaa ggtaaaacag ttgcttgtcg aagaaggcat
    11281 ggacatccgt aaagaacttt attgttcagt tctggtagat agaggaaagc aacggattgt
    11341 tttgctggct tccaccgaag gcggaatgga cattgaggaa gttgcggaaa aaactccgga
    11401 gaagattctc aaagttttcg ttgatccggc agtcggcctg cgctcatatc aggcgaatga
    11461 attagcattt ggtttaaaaa ttgacaaaat taaccccaaa ctgattcgcc ctgcagcagc
    11521 tgttttcaag gcactttatc aagcatttgt gcaggaagac tgctcactgg tcgaaatcaa
    11581 tcctctggta cttacaggtg acgggcgggt agtcgcactt gacgcaaagt tgtcttttga
    11641 tgataacgcc atgttccgtc acaaggacaa tctggccttc agagacaagg atgaggaaga
    11701 tccgcttgaa gttgaagcaa gtgaagccga cctgaactac atcaagctgg atggaagtat
    11761 cggctgtatg gtaaatggag ctggcctggc gatgggaacc atggacatca tcaaagccta
    11821 tggaggagat cccgcaaatt ttcttgatgt tggaggtgct gccagtcagg aaaatgtaga
    11881 aaaagctttc agaattatta ccatggatcc aaatgtaaag tgtattttga tcaatatatt
    11941 tggtggaatt gttcgatgcg acatggttgc cgcaggtgta attgctgctt tcaaaagtgt
    12001 tgatttgcaa attcctgtgg tggtgcgtct cgaaggaacc aattctgaag aagcacacat
    12061 tcttgtgagc aaatccggac tgggtgaaag actgcagatg gctgatggaa tccgcgaagc
    12121 cgccaaaaaa gcagttgctg cagtcagcta accagggtga tgttttttac tgaaacaaaa
    12181 cacaaacgct ttagatctga cactttccgg aacttgcaag caagtgtcct gcaaaggaaa
    12241 aactgttgca cagtgctccc ttaaagagta aaatggtcag tctaatcttt ccagtgagtt
    12301 cttacactgt caatgggggt gtcatggaat tcgattacaa gtggaaatat tgggaagcaa
    12361 gtcgtgggtg atgatcgggg tcacgtaaaa acgatcatca aaatgttagc tgcaaataac
    12421 gctgactata cacctgctcg cgtcgcactt gcggcgtaat tgaagcaggc gtctttcgtt
    12481 gagtttgcct gaaacgtagc gaaagatgtt atctaagtca ggctggctgt tgcattcctc
    12541 cgtagcagca gtaagattta aggaggtgtt tcagattgag gaggttggtt gccgacacat
    12601 ctgaagaaaa agaaaaattc caactaaact tgtagatgcc caaatggaaa cttagtaaga
    12661 cgcgggttcg a
//
LOCUS       DTVD01000014            3303 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11250_length_3303_cov_365.014778, whole genome
            shotgun sequence.
ACCESSION   DTVD01000014 DTVD01000000
VERSION     DTVD01000014.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11250_length_3303_cov_365.014778"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            615..1388
                     /locus_tag="EYO68_00480"
     CDS             615..1388
                     /locus_tag="EYO68_00480"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HIC06312.1"
                     /translation="MKTKIEITRDWLPRYTGTEISEFGEYILLTNFSNYLSKFAKRFD
                     CEIKGKGRPMQTATNSNGLSIINFGIGSANAATIMDLLTACNPKGVLFLGKCGGLKQS
                     AEIGRYIIPIAAIRGEGTSNDYLPPEVPALPSFKLHKFVSDTVLDYGQEYRTGVIYTT
                     NRRVWEWDNKFRKRLIDLSAIGIDMETATLFIVGHANKISRGAILLVSDMPLMPEGVK
                     TQEMDKKVTAKYVDMHLEMGIQAMTDIEEKGEEIKHFGY"
     gene            1421..2260
                     /gene="amrB"
                     /locus_tag="EYO68_00485"
     CDS             1421..2260
                     /gene="amrB"
                     /locus_tag="EYO68_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIC06313.1"
                     /translation="MITGNTEKNIRPPAVASMFYPGGTAELRNAVQNYLSNAGTEEDV
                     PKLKKGETAELRTLIVPHAGYIYSGKIAASAYRLLEQNQNRFKRVLLLGPAHRVWLEG
                     AAFPEADAFQTPLGEITLDKELIEKILAEFSWISVSDKAHAEEHCLEVQLPFLQETLG
                     EFKLLPLVVGDAKTELLAALIQQFSEDHETLIVISTDLSHFHDYQTAREIDARTANAI
                     ELLEQNRISTEDACGAYPLRGALLAASQNQWNVHRLGLCNSGDTAGDRGRVVGYGAWA
                     MTA"
     gene            complement(2269..>3303)
                     /gene="amrS"
                     /locus_tag="EYO68_00490"
     CDS             complement(2269..>3303)
                     /gene="amrS"
                     /locus_tag="EYO68_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIC06314.1"
                     /translation="KLDDGRIQCDVCPRLCKLHDGQRGLCFVRQAKGEQIVLTTYGRS
                     SGFCIDPIEKKPLNHFLPGTPVLSFGTAGCNLTCKFCQNWDISKSREFDRLTETASPE
                     MIAKAAKDSGCRSVAFTYNDPVIFLEYAVDIAQACRDSGVHPVAVTAGYILPEAREEF
                     FSNMDAANVDLKAFSEKFYKKICGGKLAAVLETLEYLKHETEVWFELTTLLIPGENDS
                     PEELHAMTEWIVEHLGPDVPLHFSAFHPDWKMRDIENTPHSTLSRAREIALGNGIRYV
                     YVGNVHDKSGSSTYCHQCGELLIGRDWYELSDWNLDGAGLCISCGSECPGLIENHPGD
                     WGSKRLPIRL"
BASE COUNT          933 a          710 c          755 g          905 t
ORIGIN      
        1 attattctcg tgtaatttgt cattccgggt aagctagtcg ttctagcgta acttatcagt
       61 cccgcggaag agagaatcca tgggtgcttc tacaaagcta atcctctttc aaatattgca
      121 gaccttcgat tgtcattccc gcgaagcttg tcctcggcct gattggggag cgggaatcca
      181 ggggaaactt ctacttttcc ttcatatttg tctcttggta aaaaaatgaa gcaaattgta
      241 atttggaagt ggattgtttg agatacttct gaaattatga cacactgtat aaaaatttat
      301 tttttcaaac cggacttgat ctgtgattaa tatttacgat cagtttgagt acgagaattg
      361 aagaacaact cctggattcc gggtctatgc tgcgcttcgc ccggaatgac aatggaggaa
      421 aatctaaggt atgactgtct agaaataaag tttgaaacta aaaatttgtc atcccgaacg
      481 tatgtgaggg atcttctaag atatcagtac tttgacagat ctctcccttc ggtcgagatg
      541 acatgtcaca gtcgataata ttagcacctt tcaagaatta ataactaacg agttattaaa
      601 aaaagagtaa ttttatgaaa accaaaattg agatcacccg cgactggctg ccgcgttata
      661 ccggaacaga gatttccgaa tttggagaat acattttgct gacaaatttc agtaattatt
      721 tgagcaaatt tgcaaaacgt ttcgactgtg aaatcaaggg caaaggccgt cccatgcaga
      781 ctgccacaaa cagcaatgga ctgagcatca taaattttgg aatcggctct gcgaatgcgg
      841 ctacgatcat ggacctgttg actgcctgta atccaaaagg agttttgttt ttaggcaagt
      901 gtggaggttt gaagcaatct gctgaaattg gacgctatat catccctatt gcggcaattc
      961 gcggagaagg aacgagcaat gattatctgc cgccggaagt acctgcttta ccttcattta
     1021 agctgcacaa atttgtctct gataccgtcc tggactatgg gcaggaatat cgtaccggtg
     1081 taatttacac gacaaatcgc cgtgtctggg agtgggacaa taaattccgc aagcggctga
     1141 tagacctttc tgccattggt attgacatgg aaacagctac tttatttata gttggtcatg
     1201 cgaataaaat ttcccgtggt gcaattctcc ttgtttcaga tatgccgctt atgcctgaag
     1261 gagtaaaaac tcaggaaatg gacaaaaaag ttactgcaaa atatgtggat atgcatctcg
     1321 aaatgggcat ccaggccatg accgatatag aggaaaaagg tgaggaaata aaacactttg
     1381 gttactgaca attcggcgaa tgaaaattgt gaggaatacg atgattactg gaaacactga
     1441 aaaaaatatt cgacctcctg cagtggcctc aatgttctat ccgggaggta ctgcagaact
     1501 aaggaatgca gtgcagaatt atttgagcaa tgcaggcaca gaagaagacg ttcccaagtt
     1561 gaaaaaaggt gaaactgctg aattacgcac actgattgtt ccacatgccg gttacattta
     1621 ttccggaaaa atagcggctt ccgcctatcg tcttctagag caaaatcaaa atcgatttaa
     1681 acgggttttg cttttggggc cggcacatcg ggtttggctt gagggtgcgg cttttcctga
     1741 ggcagatgcc tttcaaactc cacttggtga gataactctg gataaagagt tgattgaaaa
     1801 aatactggcg gaattttcat ggatttctgt cagtgacaag gcgcatgccg aagagcattg
     1861 tttggaagtt caactgcctt ttttacagga aactcttggt gaattcaaac tgcttccact
     1921 ggttgtggga gatgcgaaaa cagaactgct tgctgcacta attcagcaat ttagcgaaga
     1981 ccatgaaacg cttattgtga tcagtacgga cctcagtcat tttcatgact atcaaacggc
     2041 acgggaaatc gatgcacgta ccgcaaatgc aatagaactt ctggagcaaa accggatttc
     2101 aacagaagat gcctgtggag catatccgct gcggggagct ttattagccg cttctcaaaa
     2161 ccaatggaac gttcatcggc ttggcctctg taattccgga gatactgcgg gagatcgcgg
     2221 acgtgttgtc ggttatggcg cgtgggcgat gactgcctga ccctggattt ataaacgtat
     2281 cggaagcctc tttgatcccc agtctccggg atgattttca ataagtccgg gacactcaga
     2341 tccgcaggaa atacagagtc cggctccatc caggttccag tcgcttaatt cataccagtc
     2401 cctgccgatt aacaactcac cacattgatg gcagtatgtg cttgatccgg atttgtcatg
     2461 aacatttccg acatagacgt aacgaattcc atttcccaga gcaatttccc ttgcgcggct
     2521 taaagttgag tgcggagtgt tttcgatatc acgcattttc cagtccggat gaaatgcgga
     2581 aaagtgcagg ggaacatccg gaccgaggtg ctccactatc cattccgtca ttgcatgcag
     2641 ttcttccgga gagtcgtttt ctcccggaat gagcagcgtt gtcaattcaa accaaacctc
     2701 agtttcgtgc tttaaatatt ccaaagtttc caaaacagca gcaagttttc ctccgcaaat
     2761 ctttttgtaa aatttttcac tgaaagcctt caggtcaaca tttgccgcat ccatgttgct
     2821 gaaaaattct tcacgtgctt ccggaagaat atagcctgct gtgaccgcaa ccggatgaac
     2881 accagaatcc cggcaggcct gtgcgatatc cactgcatat tccaggaaaa tcaccggatc
     2941 attgtaagtg aaagcgacgc tgcggcagcc ggagtctttt gcagctttgg caatcatttc
     3001 aggagaagcg gtttcggtga gtctgtcaaa ttcacgcgac ttactgatgt cccagttttg
     3061 acaaaattta caggtgagat tacatccggc agttccaaac gataaaaccg gtgtgcccgg
     3121 aaggaaatga ttcagaggtt ttttttcaat cggatcaatg caaaatccgc tggaacgtcc
     3181 ataagttgtc agtacaattt gctccccttt tgcctggcgc acaaaacaaa gtccacgctg
     3241 cccgtcatgt agtttgcaca gcctcgggca gacgtcacat tgaatcctgc cgtcatcaag
     3301 ttt
//
LOCUS       DTVD01000015            3268 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11450_length_3268_cov_269.991285, whole genome
            shotgun sequence.
ACCESSION   DTVD01000015 DTVD01000000
VERSION     DTVD01000015.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11450_length_3268_cov_269.991285"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(193..1677)
                     /locus_tag="EYO68_00495"
     CDS             complement(193..1677)
                     /locus_tag="EYO68_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HIC06315.1"
                     /translation="MALLGSLNTGASGVRTQGKTMQIIGNNIANVNTFGFKTNQVAFE
                     DLMGNSWPQGSAFTKASNGVKIGGVAMDYSQGAFQTTTLNTDMAIAGPGFFTIVSEST
                     GKESYTRNGQFSYDKEGFLSTLRGGRVQALKVDRVTSESKGIPGALKVLGLVDAPRPT
                     GDGSRGTGLIIAANLDANAVVKDVPVDPTNVLDSMYNFATSTTVYDALGNSHAATIAM
                     RKRPDLPEQIDPGTGQPIAGTGVSNQWEYYMMFDGASLGQVPGTMVAVGGGFMQFTAD
                     GKLIAATGGSFEAQPGGVGPDGEPLPAGPPRLIPQPVDPDTGVPQFAVPFGGSSPIIL
                     GLHLGDGYNPDDPTDPRSGLDGITQFAGSYNVLQTSADGNPAGTLESIFLEDNGTVNG
                     VFDAGYTRSIGRLVLTKFDNPGKLAQVGDNMLVETLGSGKKVTGEAGTAGLGEIRSKS
                     LEQSNVDLSDEFVKMVETQRAFQASAKTVTTSDEMIQDLINMKR"
     gene            complement(1807..2586)
                     /locus_tag="EYO68_00500"
     CDS             complement(1807..2586)
                     /locus_tag="EYO68_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HIC06316.1"
                     /translation="MNIGNNVEQALQVSATGLRNRASTNSTDGKELGKQDFLNLLMTQ
                     MANQDPLDPMDSAGMMQQLAAMGTVEQLQNLNNQTAKLMAIQQQIARATAGSLLDKDV
                     EVGASEIPLKNGDSVPVAYKLEGSADRVMLLLQDSTGEMIRKINLESRAQGVHEFSWD
                     GRDNDGDVMPDGNYSFNVFARTDGGEEIGVTLTKSGQVSMIRFEGSKPLLKINGEWVP
                     ADEIVGLGNKSILRYQNAIPLPAQSELTTRKTPAWSSRSSD"
     gene            complement(2598..3251)
                     /locus_tag="EYO68_00505"
     CDS             complement(2598..3251)
                     /locus_tag="EYO68_00505"
                     /inference="COORDINATES: protein motif:HMM:PF02120.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIC06317.1"
                     /translation="MNSAEKSKDISENAFKQPLNQFRSLTSKETQLSSNVKSPVDSVS
                     APGINAVSELSGTSILKGTEVANSPNGNLRSADLPFNMEQVVSRVRILSGNGVEEMTL
                     RLHPEELGQITLKIRQAGGNLTIDMRVDNMLAKQIVESGFDSLRSKFLDQEFSYKDLA
                     LNVDINERDSKYGGDRKYAEFDEEMFSPERGNKEELSALEETPIVRHRTDSGLNLYV"
BASE COUNT          868 a          792 c          647 g          961 t
ORIGIN      
        1 agctgcttag cagcatctcc ttgaacaaca ggaaatgttt acggccgaac tcaaaccgtg
       61 tctttttgag acctgagaaa agaaaaatct cgagtgtttc agtctcgcaa gttttgacaa
      121 ctttttgggg aatttaataa aaggcagggg aaaaattgag ggccaaaaag tggccctcat
      181 tatatcggtt aattaccgtt tcatgttgat caggtcctga atcatctcat cagaagtcgt
      241 tactgtttta gcacttgcct gaaaggcacg ctgggtttca accatcttga caaattcatc
      301 agaaagatca acgtttgatt gctccagact tttactgcga atttcaccca aacctgcagt
      361 accagcctca cctgtcactt tcttgcctga acctaaagtt tctacaagca tgttatctcc
      421 gacttgagca agtttccctg gattgtcaaa tttagtcaaa acgagcctgc caattgagcg
      481 agtgtatcct gcatcaaaga caccattcac agtgccatta tcttccagga aaatactttc
      541 caaagttcct gcagggttac catctgcact ggtttgaaga acattgtagc ttccagcaaa
      601 ctgagtaata ccatccaatc cggatctggg atctgtcgga tcatccggat tatagccatc
      661 tccgaggtgc agtcctagaa ttatcggact actaccacca aaaggaacag caaattgcgg
      721 aacccctgtg tcaggatcta caggctgtgg aatcaatcga ggaggcccgg caggcaaagg
      781 ctcaccatcc ggcccaaccc ctccaggttg agcttcaaaa gaacctcctg tcgcagcaat
      841 taattttcca tcagctgtaa actgcatgaa tccgccgccc actgcgacca tcgttcccgg
      901 aacctgtccc agagaagctc catcaaacat catgtaatat tcccactgat tgctaacgcc
      961 tgttcccgca attggctggc ctgtacccgg atcaatttgt tcaggaaggt ccggacgttt
     1021 tctcatggca attgttgcag cgtgagaatt accaagggca tcataaacag tggttgatgt
     1081 tgcgaaattg tacatgctgt ccagtacatt ggttggatca actggaacat ctttgacgac
     1141 tgcattagcg tcaaggtttg cagctattat aagaccagtt cctcttgatc catccccagt
     1201 tggtcgagga gcatcaacaa gacctaaaac tttaagtgct cccggaatcc ctttactctc
     1261 actggttact cgatccactt tcaaagcctg cacacgtcct ccccgcagag tggaaagaaa
     1321 cccctcttta tcgtaagaaa actgaccgtt acgggtgtat gactctttgc cggtagactc
     1381 actgacgatg gtaaaaaaac cagggccggc aattgccata tcagtgttga gagttgtggt
     1441 ttgaaatgca ccctgtgaat aatccattgc cacaccacca attttcacgc cattactggc
     1501 ctttgtgaag gccgatccct gaggccatga atttcccatt aagtcctcaa atgcaacttg
     1561 gttggttttg aacccaaagg tgttcacatt ggcaatgtta ttaccaataa tctgcatggt
     1621 cttgccctgg gtccttactc cgctggcccc agtatttaat gatccaagta acgccatttt
     1681 atttctcctt aagctgaatt agaaacatac caggcgatac agtccattgc ttcgcgtaga
     1741 tataattaaa aaaatgaata gtttgaaact aatcgtttat ggttgaactt gcatgttcaa
     1801 ccggtcttaa tcagaacttc tggaactcca tgcaggagtt tttcgtgtcg tcagttcaga
     1861 ttgtgctgga agagggattg cgttttgata tcgcaaaatt gatttattac ctaaaccaac
     1921 aatttcatcg gcaggtaccc attcaccatt aattttcagt agtggtttac taccttcaaa
     1981 acggatcatg gatacttgac cagatttggt cagtgtgact ccaatttctt ctccgccatc
     2041 agttcttgca aagacgttga agctgtaatt cccatccggc attacatctc cgtcattgtc
     2101 acggccatcc caggaaaact catgaacgcc ctgtgctctg ctttcaaggt tgatctttcg
     2161 aatcatttca ccagtggaat cctgcaaaag gagcatgacc ctgtcggcac ttccttccaa
     2221 cttgtaggca accggtacag aatcaccatt tttcaaaggt atttcgcttg ctccaacttc
     2281 cacatcttta tccaataaac taccagcagt ggcccgagca atctgctgct gaatcgccat
     2341 caattttgca gtctgattat taaggttctg taactgttcc actgtaccca ttgctgccaa
     2401 ttgctgcatc atgcctgctg aatccatggg atcaagggga tcctggttag ccatttgagt
     2461 catcagcaaa ttcagaaaat cttgtttccc cagttctttt ccgtcggtac tgttcgttga
     2521 agcacggtta cgtaggcctg tcgcgctgac ttgcagggcc tgttcaacgt tgtttccgat
     2581 attcatattt acctttctca gacgtacaga ttaagtccgg aatcagtccg gtgcctaaca
     2641 attggtgttt cttccaatgc agataattct tctttattac ctctctctgg agaaaacatt
     2701 tcttcatcga attcagcata cttacggtca ccgccatatt tggaatcacg ttcgttaata
     2761 tcaacgttga gagctaaatc cttataagaa aattcctgat ccaaaaattt actgcggaga
     2821 gaatcaaatc cggattcgac tatttgtttt gcaagcatgt tgtcaactcg catatctatc
     2881 gtcagatttc ctcctgcttg tctgattttc agagtaattt gccccaattc ttccggatgc
     2941 aaacgaagtg tcatttcttc aacaccatta ccacttaata ttctgacacg acttaccact
     3001 tgttccatgt taaacggaag gtcagcacta cgcaggttcc catttggaga atttgcaacc
     3061 tcggttcctt ttaaaattga tgtaccggaa agttctgaaa cagcattaat tcctggtgcg
     3121 gaaaccgaat ctacaggaga ttttacgttg ctggaaagtt gagtttcttt agaggttaat
     3181 gacctgaact gattcaaagg ctgtttgaat gcattttcag aaatatcttt tgatttctca
     3241 gcactattca tttgtaagcc tgctgtgg
//
LOCUS       DTVD01000016           12537 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1145_length_12537_cov_407.755888, whole genome
            shotgun sequence.
ACCESSION   DTVD01000016 DTVD01000000
VERSION     DTVD01000016.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12537)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12537)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12537
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_12537_cov_407.755888"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(144..1310)
                     /gene="ispG"
                     /locus_tag="EYO68_00510"
     CDS             complement(144..1310)
                     /gene="ispG"
                     /locus_tag="EYO68_00510"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HIC06318.1"
                     /translation="MLPTRRHTNTVSIGSIKIGGSEPVRIQSMLTSDTTNVESCIEEI
                     ARLDAVDCEIIRLTVPNQKSIDSLPAIRTEMKKRGIERPLVADIHFNPQLAVNVCEFV
                     EKVRINPGNYVDRKKFEVREYTDLQYQEELERVEEKLLPLIQNLKKYGCALRIGTNHG
                     SLSDRLMNRFGDSPEGMVESAMEFLRILRKHNFEDTVLSMKSSNPLVMIESYRQLVRV
                     MDDENMHYPLHLGVTEAGNELDGRIKSAVGIGALLCDGFGDTIRVSLTEPSENEIPVA
                     RSILQSTRSRIFNADFISCPSCGRTFFDLESTTEQIKVHTSHLKGVKIAVMGCIVNGP
                     GEMADADFGYVGAAPGKINLFHGKTCKERNVPAEQAVERLIDLIKENGKWVDPA"
     gene            complement(1379..2698)
                     /gene="dgt"
                     /locus_tag="EYO68_00515"
     CDS             complement(1379..2698)
                     /gene="dgt"
                     /locus_tag="EYO68_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HIC06319.1"
                     /translation="MDPWKERRAPSGVQRKDDHREDYERDRARVIHSSAFRRLQAKTQ
                     ILGVLDGDFHRTRLTHSMEVAQIGRGLVLNFVKRYPELKDLLPRLEQIETTGLAHDLG
                     HPPFGHGGETALNFAMCDYGGFEGNGQTLRILTKLESHTPEYGLDLTRRNLLGVLKYP
                     VPYSKLRQKKLPKVSGTSKNTNEQQAWQPPKCYLDTEQEVLNWILEPLSDSDQKHFHE
                     QTLPTQSNHGFSFHKALDTSLLNLADDIAYGVHDFEDGVVLRLLTRDHWQEVTKHLDP
                     EWAEDNNLLQIEKKLFKHGENSGHNRKQAVGALVHALITSAELKLNEKFEEPLLGWNA
                     VLPEEPLQFLKSLKETVSRHVIQLNTVQAATYRGRKVVLSLFETLSSEAEMLLPSSFQ
                     ILWKEAKSVRDQNRIVCDYIAGMTDQYANRFYERLFLPGHGSVFDRL"
     gene            2971..3192
                     /gene="infA"
                     /locus_tag="EYO68_00520"
     CDS             2971..3192
                     /gene="infA"
                     /locus_tag="EYO68_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIC06320.1"
                     /translation="MSEDKLEVEGIVLESSKGIFRVKLENDHVVIGHLSGKMKMYKIR
                     VLPGDTVTIELSPYDLKRGRIVRREKVEG"
     gene            3293..5047
                     /gene="argS"
                     /locus_tag="EYO68_00525"
     CDS             3293..5047
                     /gene="argS"
                     /locus_tag="EYO68_00525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIC06321.1"
                     /translation="MNIFEQTICDWTAKVLNNIDDSRTWDTSSFNELVEKPPNPDMGD
                     YALPCFSFAKSLRRPPFQIAVELAEQLQSYISPETPLISIQAKDSYLNFTVSAAVMAE
                     VVLPDVFCGKYFTEPSNISRERVMIEFSQPNTHKGFHVGHMRNVALGDSLCRIFCYNG
                     YDVVGANYIGDVGAHIAKCLWFYSNYNTETPPEKFRGEWLGELYTHAVKKLDEAEGQE
                     KIKFQKEISQIQQKLEAKDEQFIQEWEKTREWSLLDFQEIYEWLDVSFDHIFYESEVD
                     EEGRQIVIEGEKKGIFQYSEGAIGIDLEEEELGFFMLLKSDGNTLYSSKDLALAQHKF
                     DQFGVKRSIYVVGAEQTLHFKQVFATLKRMGYEQVDKCFHLPYALVMLPEGKMSSRAG
                     NVILFSQLRKEIIESIQSNHLGEHRQEWSKKELEETAQKIAVAAIKYGMLNQDSNKQI
                     VFSMDDWLVSEGDTGTYLVYAYVRIRSICRQISREVVADVDFSLLAHPNEKKLLRQML
                     DFNRTVFKSGEQYRPSLLARMLYEFSKDFSRAYNTCSVKHAETEMLQAARLLLFHCVA
                     ETLLQGLHLIGISPPERM"
     gene            5124..5567
                     /locus_tag="EYO68_00530"
     CDS             5124..5567
                     /locus_tag="EYO68_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06322.1"
                     /translation="MVLEQDSTIRSFMLKWLFILLISLILSTELFADYFKWEILVSEN
                     GYGTESHTIPVDSEGKIKSAANLAECRMVSFWTRIESELLLEGKTLVCVFEDKRQSVS
                     VVCRDNHLNRKYNRVKELYPVAKDGFRINPELGAGSPYFELRCYF"
     gene            complement(5581..6276)
                     /locus_tag="EYO68_00535"
     CDS             complement(5581..6276)
                     /locus_tag="EYO68_00535"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIC06323.1"
                     /translation="MKIGILCAIPKEIHFFELLPNSGQQVGGRTFFKSKHSFHELIIV
                     ECGIGKVNSAMVSTILIQEFKCEILIFSGIAGGIDPDMEIGEVLIGEFLIQYDYGALK
                     DGNIRVYRAGKIPMGLQKNKAEFGLDPVIKEKINTSLPDVRMGTILTGDVFLQCQETR
                     KELYEKFGAQAVEMEGGAIAQVAEQFGIPAIVVRCLSDLAGANGHKLSSTSLKKAAKS
                     SFETVQSILNALL"
     gene            complement(6489..7586)
                     /locus_tag="EYO68_00540"
     CDS             complement(6489..7586)
                     /locus_tag="EYO68_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIC06324.1"
                     /translation="MSDKTVRVLFTGGGSGGPTIPLLALAQEIRGLKKNSEFLFLGSK
                     FGPERKMVEQAEIPFSTIPSGKLRRYWNWRNFIDPLYILGGGITGLIYLLRFRPQVIV
                     SAGSFVSVPVAYAAWLLRIPHVIMQMDLRSGLANRLMAPVSSALVFYFDSTASQFPSI
                     SLKRKIGPVVRQEIYSASAKRANERFGLHPERPLILITGGGQGAVGLNQAIFPLIKHW
                     LVDFQVVHLTGTKWNNSLEQESSAFSHPDYHQIETIHEGMGDLLSRSEIVLTRAGMGI
                     LGELAVLQKDTVLIPLPGTHQEDNALIIKKYKAAEIISQVDLASTGLEWWNSFLEKRV
                     PGEMGKRLHEILQDGGTNDFAQLVFEITDER"
     gene            complement(7607..8446)
                     /locus_tag="EYO68_00545"
     CDS             complement(7607..8446)
                     /locus_tag="EYO68_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIC06325.1"
                     /translation="MELPTWLETRLMQVCDLVFAVIPQQTPTLEKLGECKIISHRGQH
                     DNQEIFENTLTAFDHALETDGIWGIELDFRWTKDLYPVVSHDPDLMRLYGSNTRISET
                     KLEDLNRICPHIPTLEEVLQRYGGKLHLMVEAKEEYYPQPEKQNRILKELFSKLKPQK
                     DFHLLCLIPEMLELVTFTENSAKILVAVFNEKILSNISLNENYAGLGGHYLLLHRKLV
                     KQHLDCGQNIGVGYPRSKNNLFREINRGVEWIFCNDAVGLSSILQAELKKAQSLVVEG
                     SVN"
     gene            complement(8508..10739)
                     /locus_tag="EYO68_00550"
     CDS             complement(8508..10739)
                     /locus_tag="EYO68_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HIC06326.1"
                     /translation="MLKTAIMPVTKKTASSASDKALIAKLVKQIRSYVQEYGTVKDSE
                     LLEQAIDDIRKHHEHQKRKSGQPAIIHPLRVANYICRAGLDAPTVVAAILHDIIEDTK
                     ITHKDIKNRYGAWYADIVRGLTKIKNPESPKEGEADYLDATYQRMLKAMTQDVRALLI
                     KLFDRLDNMRDMEAMPRHKQRRISLETLNVYVPIAERLGLTQICREHTELCFKLLYPK
                     RYNKTLTEIDELKKARTSTINGMRISLLRTLEKNNLAYKTIEPLFVHPASRIQERGPI
                     DHVLEGFRIIVKNSLDCFKALGIVHTSFYVIPLKIRDYISNPLWNGYEGIQTELRIEG
                     EQTCIEIVSEEILKKNQYGIMAHWQGSPTELADYYRIYLNQLDQLAGDKDVRMLEVMS
                     YVQSDQVQVYSPKGDMFVFPKSATVLDFAYGIHSELGNHCIGALVNPSSVGTTKKRVP
                     RERQLFAGEALQILTDRGVQPREEWLEQVKTAKSRAQIKRALEQQKIISARNSGRTLL
                     ERKLRDYGDEQGTEKWIKSKAVKEALKAEKISANKFFQEIGLQKRPLKMFLRKYKLLQ
                     YQYTGRIKEMIGSEFWGNIFGGDKNIFLIEDIQNPLIRLASCCFPIPGDKITGFICND
                     HEIEIHVTNCSKLTRKNKRLQQNKIPIDVAWKISKKITRSHIIHFQVIDGTGILFQIT
                     KIIKDAGVAIINSETAAKKNKEADIRIKLESVSWPVFHKIVEKLRPLKFVKEIWEESP
                     DSV"
     gene            complement(10744..11400)
                     /locus_tag="EYO68_00555"
     CDS             complement(10744..11400)
                     /locus_tag="EYO68_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06327.1"
                     /translation="MHKKRRLNGDFHNLLNLDSLVDIVSNNVGILVILAVFMAMFSLL
                     EKNEQVISKTQSTENIEKLKIPWSHVSQKNILLIFLRSDRLLYLDRTLVYQRLKKYLS
                     GSEPLPKHVNLDQFSIELTVGSGHAHCLKFLPSPRAGQWWHQFSQRNGVMKKLMKKYP
                     PEENYFFFWVDPDSFEMFREIRKSLWDLNFEVGWKPIRIETPLRYCSGVGQAREFLPQ
                     "
     gene            complement(11400..>12537)
                     /locus_tag="EYO68_00560"
     CDS             complement(11400..>12537)
                     /locus_tag="EYO68_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C14 caspase catalytic subunit p20"
                     /protein_id="HIC06328.1"
                     /translation="DDKDDLDSIDAVQTFKYITSNLEAESLQEKEAIFTLGIYCLKNN
                     ETKIAQYSLKRLLMRDKENVDLRCFLVLAISIDGKINEAIKSFQSILGRNPNHRYTNV
                     NMGILLKRKGMIQQSRVRFFTTFRLLEQSKGNYDMKACLENADKLFNNNQKKKALEIY
                     EPLISEITSEVLLHRIAKLYLDKKLLDDALEIYKRIFRKNRQNIEAREGIKSIHTAYL
                     IESENFLKKNDKINAAVKIEKALKIAAGINLIQKAISINLLLKNENRVVELEEMLKDI
                     KRKEIQSKVQEKINKAEEAEKRTDYKGAIRYFQEAIKIEPQNSTLKKLIDLCVRINRP
                     DLTEKITDWFNKYQHSLLEKEKKQAREELKQSKNNGDESQKDGI"
BASE COUNT         3584 a         2541 c         2393 g         4019 t
ORIGIN      
        1 atgatgaacc tgtaaaaagt cctaaatcct tgtcatttcg agcgaagcga gaaatcttgc
       61 atatgctact atactaatat cattaagatt tctcccaacg gtcggaatga catattgttg
      121 atttcagact tattacgaaa ccatcaagca gggtcaaccc atttcccatt ctccttgatg
      181 aggtcaatta atcgttccac agcttgttca gcgggtacat tgcgttcctt acatgttttt
      241 ccgtgaaaga gattgatttt gccaggagca gcgccaacgt agccaaaatc ggcatctgcc
      301 atttcacccg gaccgtttac tatgcacccc atgaccgcaa ttttaactcc cttgaggtgt
      361 gaagtgtgta cttttatttg ttcagtggta ctttctaagt caaaaaacgt acgaccgcat
      421 gatggacaag aaataaaatc tgcattaaaa attctggatc gtgtggactg taaaatagaa
      481 cgggctacag ggatttcatt ttctgacggt tctgtaagtg aaactctgat tgtgtctcca
      541 aaaccgtcgc aaagcaaggc gccgattccc actgcacttt ttatacgtcc atccagttca
      601 ttacctgctt cagtaactcc caaatgcaga ggataatgca tgttttcatc atccattact
      661 cgtacaagct gccgatatga ctcaatcatc accaggggat tagaggattt catggacagt
      721 actgtatcct caaaattatg cttccggaga atacgtaaaa attccatagc agattcaacc
      781 attccttccg gcgaatctcc aaagcggttc atcagtcgat ctgagagtga accgtggttt
      841 gtaccaattc tcagagcgca tccgtatttt ttcaggtttt gtatcagcgg aagcaatttt
      901 tcttcaacac gttccaattc ttcctgatac tgcagatcag tatattcgcg tacttcaaat
      961 tttttccggt cgacataatt gcctggatta atgcggactt tttccacaaa ttcacataca
     1021 ttaactgcca attgcggatt aaaatgaatg tccgctacca ggggacgttc gattccacgc
     1081 ttcttcattt cagttcggat tgcgggcaga ctgtcaattg atttttgatt cgggactgtc
     1141 aggcggataa tttcacaatc cactgcatct aatcgtgcaa tttcttcaat gcatgattca
     1201 acattggtgg tgtcagaggt cagcattgac tgaatccgaa caggttcaga acctccaatc
     1261 tttatggaac cgattgaaac agtgttggta tgacgtctag tggggagcaa tttagcttat
     1321 tgtgttttgg gtttaagttt ttattgagtg tgaaaaaatt tctgcactca acatagtttc
     1381 atagtctgtc aaacacggag ccatgtccag ggagaaacag gcgttcataa aagcgatttg
     1441 cgtattgatc tgtcatgccg gcaatatagt cacaaactat tctattttga tcccgaacac
     1501 tttttgcctc tttccataaa atctgaaaag atgaaggtaa aagcatctca gcttcagatg
     1561 agagtgtttc aaacagggaa agcacaacct tacgtccccg atatgttgca gcttgtacag
     1621 tatttagttg gatgacatga cgcgaaacag tttctttcag tgatttgagg aattgcagtg
     1681 gttcttccgg aagaactgca ttccatccca ggaggggttc ttcaaatttt tcattaagtt
     1741 tcaattctgc agaagtaata agtgcatgaa ccagtgctcc aacagcttgt ttgcggttgt
     1801 gcccggagtt ttcaccatgt ttgaaaagtt ttttttcaat ttgcagaaga ttattatcct
     1861 ctgcccattc tggatccaaa tgttttgtga cttcctgcca gtggtcacga gtcaacaatc
     1921 gcaaaacaac accatcttca aaatcatgaa ccccataagc aatatcatct gccaaattca
     1981 acagtgaggt atccagagct ttgtggaacg aaaaaccatg atttgattga gtaggtaaag
     2041 tttgctcgtg gaaatgtttt tggtctgaat ctgaaagggg ttctaaaatc cagttaagca
     2101 cttcctgctc tgtatccaga taacactttg gaggttgcca agcttgttgc tcatttgtat
     2161 ttttagaagt gccggatact ttcggcagtt ttttttgacg aagtttggaa tagggtacag
     2221 gatattttag aactcccaga aggttacggc gcgttaaatc taatccatac tcgggtgtgt
     2281 gtgattccag ttttgtgaga attcgcaatg tctgtccatt gccttcaaat cctccatagt
     2341 cgcacattgc aaaattgaga gcagtctctc cgccgtgtcc aaaaggtgga tgtcccaaat
     2401 catgggccaa tcctgtcgtt tctatctgtt ccagtcgggg aagcaggtct ttgagttccg
     2461 gatatcgctt tacaaaatta agtacaagac ctcgtccaat ttgagctact tccattgaat
     2521 gtgtcagacg tgtacgatga aaatctccgt ctaacactcc gagtatttgt gtttttgcct
     2581 gcaatcttcg gaatgctgaa gaatgaatta cccgtgctct gtctcgctca taatcctcgc
     2641 ggtgatcgtc tttccgttga acaccggaag gagcgcgacg ttctttccat gggtccattt
     2701 aaacgtttct atccggatgt gagaataatt tatgtttgaa ttttttttat tgactatcat
     2761 ggattataac ggattttaat tagattttca ccggataaaa agaagatata aaaaccggat
     2821 gcatgctaat caattttcgg ttaaggagac aagaaagtct gaactcttca aatcacttga
     2881 tatatccttg aatttaatta gtataagttg ttaaatcttt atattaaact aaaataactg
     2941 agataatttt ttagtaaagg agaacagcat ttgagtgaag ataaactaga agtcgagggg
     3001 attgtacttg aatcctccaa aggcattttt cgggtaaaac ttgaaaatga tcatgttgtt
     3061 attggccatt tgtcgggaaa aatgaaaatg tataagattc gtgtacttcc cggagataca
     3121 gtcacaatag aactttcccc ctatgatctg aaacgtggaa gaatagtgcg gcgggaaaaa
     3181 gttgagggtt gaaagaaatt ttatctttaa agtttcatta ataatgatgg aaagcaggtt
     3241 tgttgggtac tgtggataac tggtttaccc aagatttaac gattgatctt ggatgaatat
     3301 atttgaacaa acgatctgtg attggaccgc aaaggttcta aacaacattg atgattcaag
     3361 aacttgggat acatcatcat tcaacgaatt agttgagaaa ccacccaatc ctgatatggg
     3421 agattatgca ctcccatgct ttagctttgc taaaagtttg cgacgccccc cctttcaaat
     3481 tgctgtggaa ctggcagagc agcttcaatc gtatatctca ccggaaaccc ctttaatttc
     3541 aatccaggca aaagactcct atctgaattt tactgtttct gctgctgtaa tggctgaagt
     3601 agtcttacct gatgttttct gtggaaagta tttcacagag ccctccaaca tttcccgtga
     3661 acgggtgatg attgaatttt ctcaaccaaa tacacacaaa ggttttcatg ttggacatat
     3721 gcgtaatgtg gcgcttggag atagtttgtg ccgtatcttt tgttacaacg gctatgatgt
     3781 agtcggagca aactacattg gtgacgttgg agcacatatc gcaaaatgtt tgtggtttta
     3841 tagtaattac aacactgaaa ctcctccgga aaaatttcgt ggagagtggt taggtgaact
     3901 ttacacgcat gcagtaaaaa aactggatga agcagaaggt caagagaaaa ttaagtttca
     3961 gaaagaaatc agtcaaattc agcaaaaact ggaagcaaaa gacgagcaat tcattcaaga
     4021 atgggaaaaa acgcgggaat ggtctttgtt ggattttcaa gaaatatatg agtggctgga
     4081 tgtaagtttt gaccatattt tctatgagtc cgaggttgat gaagaaggca ggcaaattgt
     4141 aattgaagga gaaaaaaaag ggatatttca gtattcagaa ggtgcaattg ggattgatct
     4201 tgaggaggaa gaacttggtt tttttatgct cctgaaatca gatgggaaca ccctctattc
     4261 ctcaaaagac cttgcacttg cacaacacaa atttgaccag tttggagtga agcgctcaat
     4321 ttatgtggtt ggggctgaac agacgctgca ttttaaacaa gtttttgcca ccctcaaaag
     4381 aatgggctat gagcaagtcg acaagtgttt ccatttgccg tatgcacttg tgatgcttcc
     4441 agaaggtaaa atgagttctc gtgcaggaaa tgtgattctg ttttcgcagt tgcgcaaaga
     4501 aatcattgaa agtatccaat ccaatcatct aggagaacat cggcaggaat ggagtaagaa
     4561 agaacttgaa gaaacggctc aaaaaattgc agtggcagcc attaaatacg gtatgctaaa
     4621 tcaagactca aataagcaga ttgtgttttc aatggacgac tggctggttt ctgaaggaga
     4681 taccggcacc tatctggttt atgcctacgt gcgaatcagg agcatttgtc ggcaaatttc
     4741 gcgtgaagtg gtcgctgatg tggacttttc actattagca catcccaatg aaaaaaagct
     4801 tttacgacag atgctggatt tcaatcgcac tgtatttaaa tccggtgagc aataccgtcc
     4861 ttccttactt gcccggatgc tctatgagtt ttccaaagat ttcagccgtg cctacaacac
     4921 atgttcagta aagcatgctg aaactgaaat gctgcaagct gccagattac ttttatttca
     4981 ctgtgttgct gaaactctcc tgcagggtct tcacctcatt ggaatctcac caccagaacg
     5041 gatgtgacaa gattggaaat atttttggat taaagacttt aggattccag gaccattcaa
     5101 gaaaactata ttttgtgtct tttatggtac tggaacaaga ttctacaata agatcattta
     5161 tgcttaaatg gttgtttatt ttactaattt cactcatcct ctctacggaa ttatttgcag
     5221 actattttaa atgggagatt ctggtttccg aaaatggtta cggaactgag agccacacta
     5281 tccctgttga ttcagaagga aaaatcaaat ctgcagcaaa tttggctgag tgcaggatgg
     5341 taagtttttg gacccggata gaatctgagt tgctgctgga aggaaaaact ttggtctgtg
     5401 tttttgaaga caaaaggcaa tccgtcagcg ttgtttgcag agacaatcac cttaaccgca
     5461 aatacaacag ggtcaaagag ctttatccag ttgcaaaaga tggatttcgg atcaatccgg
     5521 aattaggcgc aggttcaccc tattttgaac tgcgctgcta tttttgaaga tctgctgcaa
     5581 ttagagcaaa gcattcaaaa tggactgcac agtttcgaat gaactctttg cggctttttt
     5641 caatgatgta gaggaaagtt tgtgtccatt tgctccagct aaatcactga gacaacgtac
     5701 cacgattgca ggtattccaa attgttctgc aacctgagca attgctcctc cttccatttc
     5761 aacggcctgc gcaccaaatt tttcatacaa ctctttcctg gtttcttgac actgaagaaa
     5821 aacatctccg gtcaggatag tacccatccg gacatctgga agtgaggtat tgattttttc
     5881 ctttatcacc ggatctagac caaattctgc tttatttttc tgtagcccca tcggtatttt
     5941 ccctgcacgg taaactcgga tatttccgtc tttcagtgca ccgtaatcat actgaatcaa
     6001 aaactcacca atcaaaactt ctccaatttc catatccgga tcaatacctc ccgcgattcc
     6061 ggaaaaaata agaatttcac acttaaattc ctgaattaga atcgtggaaa ccatcgctga
     6121 attgactttc cctatcccac attcaactat aatcagctca tggaaagaat gtttgctttt
     6181 gaaaaaagtt ctaccaccaa cctgctgtcc agaatttgga agcaattcaa aaaaatgtat
     6241 ttctttgggg atggcgcaaa ggattccaat tttcatcaaa gacacaaata taattgacta
     6301 aaaatatttt ttacaaatgg ccgggaattc tctttttttg caccactcgt aaaacttccc
     6361 gcttccaagt gcacaggacg tggtaccaaa gaataataaa aggattacga tccacaaacc
     6421 tttactatgt cgtacttgca aaactagcgc attgaaaact ttgcaacatt ttgattttag
     6481 agccattttc acctttcatc agttatctca aaaaccaact gtgcaaaatc attcgttcca
     6541 ccatcctgca gaatttcatg aagccttttc cccatttctc ccggaacacg tttttccaaa
     6601 aaactattcc accactctag gccagtggaa gctaaatcta cctgcgaaat tatttctgca
     6661 gccttgtatt ttttgataat caaagcatta tcttcctgat gtgttcccgg aagtggaatg
     6721 agaacagtat ctttttgtaa aacagcaagt tcaccgagaa tccccattcc tgcgcgtgtc
     6781 aacactattt cactcctgga aagcaagtca cccatgcctt catggatagt ttctatctgg
     6841 tggtaatccg gatgagaaaa agcggaagat tcttgttcca aagaattatt ccattttgtt
     6901 ccagtgaggt gtacaacttg aaaatcaaca agccaatgtt ttatcaaggg aaagatggct
     6961 tgattcaaac caactgctcc ctgaccgcct cctgtgataa gtataagcgg gcgttccgga
     7021 tgtagaccaa aacgttcatt tgcacgtttt gcactggcag agtatatttc ctgacgtaca
     7081 accgggccta tttttcgttt caaagatatg gaaggaaact gactggcagt actatcaaaa
     7141 tagaatacaa gcgcactgct gactggagcc atcaagcggt tagccagtcc ggagcgcaaa
     7201 tccatttgca taatcacgtg agggatcctt aaaagccagg cggcataagc taccggtaca
     7261 ctaacaaaac ttcctgcaga aacaataacc tgtgggcgga aacggagcaa ataaatcaaa
     7321 cctgttattc cacctccgag gatatacaaa ggatcgataa aatttctcca gttccaataa
     7381 cgcctgagtt tccctgaagg aatggtggaa aacggtatct cagcttgctc taccattttt
     7441 ctttccggac caaatttact gcctaagaaa agaaattcgg agttcttttt tagtcctcgg
     7501 atttcctgtg ccaaagccag caggggtatg gttggaccac cagaacctcc ccctgtgaat
     7561 aaaactcgaa cagttttatc actcataact tgcgtctctt ttcctgttag ttgacacttc
     7621 cttccacaac gagtgattga gccttcttca attctgcttg aagaatgctg ctcaaaccta
     7681 ctgcgtcatt gcagaaaatc cattccactc cacggttaat ttctcgaaaa agattatttt
     7741 tagacctggg ataaccaact ccaatatttt gtccgcagtc caaatgttgc tttactaatt
     7801 ttctgtgaag taacagataa tgcccaccaa gaccggcata gttttcattt aacgagatat
     7861 tacttaatat tttttcatta aaaaccgcaa ccaaaatttt tgctgaattt tcggtaaaag
     7921 tcactaattc caacatttct ggaatcaggc agagcaaatg aaaatctttt tgaggtttta
     7981 acttagaaaa aagttctttc agtatcctgt tttgtttttc gggttgagga taatattctt
     8041 ctttggcctc caccattaaa tgcaattttc ccccgtagcg ctgaagtact tcttccagtg
     8101 ttggaatatg agggcagatt cgatttagat cttccagttt tgtttctgaa atcctggtat
     8161 tagaaccgta caatctcatt aaatccggat catgggaaac aactggatac aagtcttttg
     8221 tccagcgaaa atccagttca ataccccaaa tcccatccgt ttctagcgca tgatcaaatg
     8281 cagtcaaagt gttttcaaaa atttcttggt tgtcatgctg cccgcggtga gaaataattt
     8341 tacattcccc taatttttcc agagtaggag tctgttgggg tatgacagca aagaccaagt
     8401 cacaaacctg catcaagcgg gtttccagcc aggttggaag ttccataaaa aattagagtg
     8461 ctattctttt atttttttgt acacaaacct ctaaaatttc cataccacta aacactatcg
     8521 ggagattctt cccagatttc ctttacaaat tttaatggtc tcaacttctc aactatttta
     8581 tgaaaaactg gccaggaaac cgattcaagt tttatacgaa tatctgcctc tttatttttc
     8641 ttagcggcgg tttcggaatt aattattgct acacctgcat cctttattat tttcgttatt
     8701 tgaaaaagaa tccctgttcc atcaatcact tggaaatgaa taatgtgaga acgagttatt
     8761 tttttagaga ttttccaagc cacatcaatt ggaattttgt tttgctgcag acgtttattt
     8821 ttacgagtta atttggagca gtttgtgacg tgtatttcaa tttcatgatc attacagata
     8881 aaacctgtga ttttgtctcc aggaattggg aaacaacaac tagccaatcg gattaaggga
     8941 ttttgaatat cttcgatcaa aaaaatgttt ttgtcgccac caaatatatt cccccaaaat
     9001 tctgaaccta tcatctcttt tatacgacca gtatattgat actgaagcaa tttatatttt
     9061 ctgagaaaca ttttcagagg acgtttttgc agtccaattt cctgaaaaaa tttgtttgct
     9121 gatatttttt ctgctttaag tgcctctttt acagccttgg atttgatcca tttctctgtg
     9181 ccttgctcat caccataatc tcgcaatttc cgctccaata atgtccgccc agaatttctg
     9241 gcactgatta ttttttgttg ttccaaagca cgtttaattt gggcacggct ctttgcagtt
     9301 ttgacttgtt caagccactc ctctcttggc tgaactccac ggtctgtgag aatctgcaaa
     9361 gcttcccctg caaacaactg acgctcacga gggacccttt tttttgttgt tcctactgaa
     9421 gaaggattta ccagggcacc aatgcaatgg tttccaagtt ctgaatgaat cccataagca
     9481 aaatctagca cagtagcact ttttggaaaa acaaacatat cacctttggg actatagacc
     9541 tgtacctgat cagactgtac atagctcata acttcgagca tgcgaacgtc tttatcacca
     9601 gccaactgat caagttgatt gaggtatatc ctgtaatagt cagctaactc agttggactt
     9661 ccctgccaat gagccatgat tccatactga ttttttttca gtatttcttc tgaaacgatc
     9721 tcaatacatg tttgttcacc ctcaattctt aactctgtct ggattccttc ataaccattc
     9781 cagagagggt tgctgatgta atctctaatt ttcaaaggaa ttacataaaa acttgtatga
     9841 actattccaa gtgctttaaa acagtccaag ctgtttttga caataatcct gaaaccttcc
     9901 aatacatggt caataggacc tctttcctga attctggaag caggatgaac aaatagcggc
     9961 tcgatcgttt tatatgctaa attattcttt tcaagagttc tcagcaaaga aattctcatg
    10021 ccatttattg ttgaagtacg ggcttttttc agttcatcaa tttctgtgag tgttttatta
    10081 tatctctttg ggtacagtag tttgaaacag agttcggtgt gttcccggca aatctgtgtt
    10141 agtcccagtc tttcggctat aggtacatat acatttagag tttcaaggct gattctacgc
    10201 tgtttgtggc ggggcatggc ttccatgtct ctcatgttat cgagtctgtc aaacagcttg
    10261 atcagcaaag cacgaacatc ctgtgtcatg gctttcaaca tacgctgata agttgcgtct
    10321 aaataatctg cttctccttc ttttggactt tccggatttt ttattttagt taaccctctg
    10381 acaatatctg cataccaggc accatatcga tttttgatgt ctttgtgtgt aattttagta
    10441 tcttcaatga tatcgtgaag aatggctgct acaactgttg gggcatccag tcctgctcga
    10501 cagatatagt ttgccacacg aagggggtga ataatggccg gttgtccgga tttgcgtttt
    10561 tgatgttcgt ggtgtttccg gatatcatca atggcttgtt caagcaactc tgaatcttta
    10621 actgtaccgt attcctgaac atagctccga atttgcttga ctaactttgc aatcaaggct
    10681 ttatcactgg ctgaactagc tgtctttttg gtgacaggca ttattgctgt tttgagcata
    10741 ttgttattgt ggtagaaatt ctcgtgcttg accaacccca ctgcagtaac gcaaaggggt
    10801 ttcaattcgg attggcttcc aacctacttc aaagtttaga tcccacaaag attttcttat
    10861 ttcacggaac atttcaaaac tgtctggatc aacccagaaa aaaaagtagt tttcttccgg
    10921 aggatatttt ttcattaatt ttttcatcac cccattacgc tgagaaaact gatgccacca
    10981 ctgtcctgct ctcggagaag gaagaaattt aagacagtgt gcgtgtccac ttccgactgt
    11041 gagttcaatg gaaaattgat ccagattgac gtgttttggt aaaggttcac taccagagag
    11101 atattttttt agcctttgat aaactaatgt acgatctagg tacaataaac ggtcactccg
    11161 cagaaaaatc aaaaggatgt ttttttgtga tacatgagac cagggaattt tcagtttttc
    11221 aatattttct gtcgattgtg tttttgaaat tacctgctca tttttttcca atagtgaaaa
    11281 cattgccatg aacacagcta atataacgag tattcctaca ttgttggaaa caatgtcaac
    11341 caatgaatcc aagtttaata aattatgaaa gtcgccattt aatctccgtt ttttgtgcat
    11401 taaattccat ctttctgaga ttcatcacca ttatttttgc tttgcttcaa ctcctcacga
    11461 gcttgttttt tttctttttc cagaagagaa tgctgatact tgttaaacca atctgttatt
    11521 ttttcagtta aatcaggacg attgatgcgg acgcaaaggt caattaattt ctttaatgtg
    11581 ctgttttgtg gttcaatctt gattgcctct tgaaagtaac ggatagctcc tttgtaatca
    11641 gtcctttttt cagcttcctc tgctttattg attttttcct gaactttact ttgaatctct
    11701 tttcttttga tatctttcag catctcctcc aactccacta ctctattttc atttttgagc
    11761 agcaggttga tactgatagc tttttgaatc agattaatac ctgcggcaat ttttagagct
    11821 ttttcaattt tcactgctgc atttattttg tcattttttt taagaaagtt ttcggattca
    11881 atcaaatatg ctgtatgaat tgatttaata ccttcacggg cctctatatt ttgacggttt
    11941 ttacgaaaaa tacgtttata aatttcaagc gcatcatcca ataatttttt atcaagataa
    12001 agcttggcga ttctatgcag caatacttcc gatgtgattt cagaaataag tggttcataa
    12061 atttcgagag cttttttctt ttgattgttg ttgaaaagtt tatctgcatt ttccaaacag
    12121 gccttcatat cgtagttccc cttagactgc tcaagtagcc tgaaagtagt gaaaaaacga
    12181 actcgggatt gctgaatcat tccttttcgt tttaacagga tccccatgtt tacattggtg
    12241 tagcgatgat taggatttcg accaagtatg ctttgaaaag atttaattgc ttcatttatc
    12301 ttaccatcga ttgaaattgc aagaacaagg aaacaacgta aatccacatt ttctttatcc
    12361 cgcatcagca atcgtttcaa agaatattgg gcgatctttg tttcattatt tttcaaacaa
    12421 taaatgccaa gtgtgaaaat agcttccttt tcttgtaagc tttctgcctc cagattggaa
    12481 gtaatgtatt tgaaagtttg aacagcgtca atactgtcta aatcatcctt gtcatca
//
LOCUS       DTVD01000017            3259 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11493_length_3259_cov_437.838015, whole genome
            shotgun sequence.
ACCESSION   DTVD01000017 DTVD01000000
VERSION     DTVD01000017.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11493_length_3259_cov_437.838015"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            263..727
                     /locus_tag="EYO68_00565"
     CDS             263..727
                     /locus_tag="EYO68_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC06329.1"
                     /translation="MQTLEINGKKYQIDVEAEMPLLWVLRDDLGLTGTKFGCGIGECG
                     ACTVHINGQAVRSCSFPVAAAVGTKITTIEGLSHHNKHPVQQAWLEEQVPQCGYCQSG
                     QIMATAALLAKNPKPTAEEVEDNITNLCRCGTYVRVRRGISRSAELMLKQGV"
     gene            729..2861
                     /locus_tag="EYO68_00570"
     CDS             729..2861
                     /locus_tag="EYO68_00570"
                     /inference="COORDINATES: protein motif:HMM:PF02738.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC06330.1"
                     /translation="MKKQSFKRRTFIKTSTFAGAGLALGFTFVPLVNSATGGRVSVSG
                     EKGAELGLWIRIAPDGTITLIAPSSEMGQGVNTSLSMILAEELEADWQSIKTETAPVN
                     SDYKNPEHPMQITGGSTSTKGFWEPLREVGAAAREMLITAAAQKWGVSVGACKAQSGY
                     IIHQKSSRKLGYGELVEAAGKLDVPSDPTLKAPEEYSLVGKPVPRLDLPDKINGTAKF
                     GIDVRLPGMLFATVRQSPVFEGTVLSHDETAAKSIRGVTAVVSIPNGIAVVANNTWRA
                     KKGMEALNPQFQDGLTKGLDCQKVRKQFRKALDNKGKVELDSPKVLDLEYEVPYLAHA
                     TLEPMNCTASVTDNFCEVWAPTQNQEMSLDVTKEITGLDEDQIRIHTTLLGGGFGRRI
                     EIDYVTQAVTISKNLGKPVQVTWMREEDMQHDFYRPASMSRFQISLGKNGLPEKWKNQ
                     LAAPSILKRYFAPLGWFGFDPTSTEGAKELPYAIPDFDFDFSMVDPGVPVGFWRSVGS
                     SHNAFYIESAIDEASHLAQHNPFEYRRKLLAEKPRYLNVLEKVAQDAKWGRRLQKGHG
                     LGIAIHKSFGSIVGAVIEISSNTSGRLELHNVWISVDCGKVVNPDIVRAQMEGGFIFG
                     LSAAVGEEITLKAGRVEQSNFHDYSVLRMKSTPDISVSIIESGEDIGGVGEPSVPLAA
                     PALANAIFSATGKRYRSLPFNKHGVKFV"
     gene            2915..>3259
                     /locus_tag="EYO68_00575"
     CDS             2915..>3259
                     /locus_tag="EYO68_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06331.1"
                     /translation="MPHLLINWKSFFCVILIFLCVVETAFAGKWRLRAREHFDTVTLK
                     YGNQIEKETTSGIGPTINFWWEEAYENSFGLALGLMYIDFARESESIGRGQRMELWKL
                     GFEGKHDPLQEDG"
BASE COUNT          993 a          636 c          794 g          836 t
ORIGIN      
        1 cacaaatccg gattactacc ggaaaaaact tgaaagaatt acgggtcaat agcaaaactc
       61 gtcttactgt ccaccaactg ttatattatg ttgaacttat aaaaaatcaa aattacaaac
      121 ttgtttttca ttcctatgaa aaagggaagc tattatttaa gatgcatacg agttcctgaa
      181 ttacagatat cgatgggaaa tacaattttt caggaagttg acacactata aaaattaatc
      241 cttaaataat tattaggaag acatgcaaac attggaaata aatgggaaaa aatatcaaat
      301 agatgtggaa gccgagatgc ccctgctttg ggttttgcgt gacgatctgg gtttgactgg
      361 aactaaattt ggctgcggca taggagaatg tggagcctgt acagtacata tcaacggaca
      421 agcagtacgc tcgtgtagtt tcccggttgc tgcagcggtg ggcaccaaaa ttacaactat
      481 agaagggctt tcccaccaca ataaacatcc ggtgcaacaa gcatggcttg aagaacaagt
      541 tccgcagtgc ggctattgtc agtccggaca aataatggcc actgcggcat tgttggcaaa
      601 aaatccaaaa ccgactgctg aggaagttga ggataatatc acaaatttgt gtcgttgcgg
      661 gacttatgtc cgggtgcggc ggggaattag ccgctctgca gaactgatgc tgaagcaagg
      721 agtttgaaat gaaaaaacag tctttcaagc gtcgaacttt cataaaaact tccacttttg
      781 ccggggcagg tttggcactt ggatttactt ttgttccctt ggtgaattca gcaactggtg
      841 gtcgagtttc ggtttcagga gaaaaaggtg cggaactagg actgtggatt cggattgcgc
      901 cggatggaac cattacactg attgctccca gctctgaaat ggggcaaggg gtgaatacct
      961 cactctccat gatattggca gaagagctgg aagcagattg gcaaagtata aaaactgaaa
     1021 ctgcacctgt taattcggat tacaaaaatc cggaacatcc tatgcaaatc accggcggca
     1081 gtaccagtac taagggattc tgggaaccac tacgtgaagt tggagcggca gcacgggaaa
     1141 tgcttataac ggcggcggcc caaaaatggg gtgtcagcgt tggggcatgt aaggcacaat
     1201 ccggatacat tattcatcaa aaaagtagtc gaaaactagg ttatggcgaa ttagtcgaag
     1261 ctgccggaaa actggatgtc ccttcagatc caacactaaa agctccggaa gaatatagtt
     1321 tggtaggcaa accggttcca cgacttgatc ttccggacaa gatcaacgga accgccaaat
     1381 tcggaattga tgtacgtttg cccggaatgc tttttgcgac tgtccggcaa tctcctgttt
     1441 ttgaaggaac agtgttgtct cacgacgaaa ctgcagcaaa aagtatccgc ggcgtgacag
     1501 cagttgtttc aattccaaac ggaattgcag tagtggcaaa taatacctgg cgggcaaaaa
     1561 agggtatgga ggcactgaat ccgcaatttc aagacgggtt gacaaaggga cttgactgtc
     1621 agaaggtccg taaacaattc cgtaaggcac ttgataacaa aggaaaggtt gagcttgatt
     1681 caccaaaagt acttgatttg gaatatgaag tcccatacct ggctcatgcc acactggaac
     1741 cgatgaactg tactgcatct gtaacagata atttttgcga agtttgggca ccaactcaaa
     1801 atcaagaaat gtcacttgat gtcacaaagg aaatcacagg tttggatgaa gatcaaatcc
     1861 ggatacacac caccttgctc ggcggcggtt ttggacgccg cattgaaata gactacgtga
     1921 cccaagcagt tacaatttcc aaaaatttag gaaaacccgt acaggtgacc tggatgcggg
     1981 aagaagacat gcaacatgat ttttaccgtc ctgccagcat gagtcgtttt caaatcagtc
     2041 tgggcaaaaa tggactgccg gaaaaatgga aaaatcaatt agctgcacca tcgattttaa
     2101 aaagatattt tgctccgctg ggatggtttg gatttgatcc aacttccaca gaaggtgcga
     2161 aggaactgcc gtatgcaatt cctgattttg attttgattt ttcaatggtt gaccccggag
     2221 ttccagttgg attttggcgt tcagtgggaa gttcccacaa tgcattctat atagaaagtg
     2281 ctatcgacga agcttctcat ttggctcaac ataatccgtt tgaataccga cgcaaacttc
     2341 tggcagaaaa accccgttac ctgaatgttt tggaaaaagt ggcgcaagat gccaaatggg
     2401 gacggagact tcagaaggga cacggattgg gaatcgcaat tcataaatcg ttcggaagca
     2461 ttgtcggagc agtgattgaa atttcctcga atacatccgg aagattggaa ttgcacaacg
     2521 tgtggatctc tgttgactgc gggaaagtcg tcaatccgga tatagttcgg gcacagatgg
     2581 aaggtggatt tatttttgga ctttcagcgg ctgttggcga agaaattact ttaaaggccg
     2641 gacgagtgga acaaagtaat tttcacgatt actccgtttt acggatgaaa agcactccgg
     2701 acatttctgt cagcatcatc gaaagtggtg aagacatagg aggtgtagga gagccctctg
     2761 ttccattggc cgctcctgct ttagccaatg ccattttttc tgcaacagga aaacgttatc
     2821 gttcattgcc gtttaacaaa catggtgtta agttcgtttg acaataaaac tgtctgtacc
     2881 cgaaaatacc agccattacc gcaatacaag caaaatgccg catttattaa taaattggaa
     2941 gagttttttc tgtgtgatct tgattttttt gtgcgtggtt gaaactgcat ttgcaggaaa
     3001 atggaggctc agagcaaggg agcattttga tactgtaacg ctaaaatacg gtaaccagat
     3061 tgaaaaagaa acaacttccg gaattggtcc gacaataaat ttttggtggg aagaggctta
     3121 tgagaactca ttcggactgg ctcttggttt gatgtacatt gattttgcaa gagaatctga
     3181 aagcatcggc agaggacaac gtatggaact ctggaagctt ggatttgagg gcaaacatga
     3241 tccactccag gaagatggg
//
LOCUS       DTVD01000018            3247 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11562_length_3247_cov_476.979950, whole genome
            shotgun sequence.
ACCESSION   DTVD01000018 DTVD01000000
VERSION     DTVD01000018.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11562_length_3247_cov_476.979950"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            520..1062
                     /locus_tag="EYO68_00580"
     CDS             520..1062
                     /locus_tag="EYO68_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06332.1"
                     /translation="MYWEKIWQLRFFRAVTITIFYFMLFLGTAQAQEKEMSAETEAQS
                     EKSPSSEYTWHYAAVSVTLVSALMSYNAAKSYNDLSAKNSTLATQYANSSSSSEQASY
                     KSEYDSNASKMKSYKSSMQTWDILTLLGLGWEAYLLRSDESGSSAVNSGSIQFPFIPR
                     LAYQTYPYGTKTVLRWNMSF"
     gene            1373..2245
                     /locus_tag="EYO68_00585"
     CDS             1373..2245
                     /locus_tag="EYO68_00585"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06333.1"
                     /translation="MPRQPTRNDYIVLFALGALWGSSFGAIKIALHGVTPLTVMSVRI
                     LLAGAALLLLIRVRKTPFPRGIQNWIKIGWMALFGTLIPFFLVPWGQLQIDSSLAAIL
                     MAVNPLFALTLGHFFSDHESFTLRQLLAMLVGFSGILLVFGENAISSINGNIWAQLAV
                     IGAGFCYTISGVIVSRVRGASADSVSASIFICSSVIVFPLWMILEQPWSLHFETESLL
                     ALTHLGLVSTGMAFLMRYYIILRAGAVFLSYVAFIIPMFGILFGILFLGETISVNTMG
                     AVVLILSGVYLGRK"
     gene            2308..3147
                     /locus_tag="EYO68_00590"
     CDS             2308..3147
                     /locus_tag="EYO68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIC06334.1"
                     /translation="MYSSLITPKIANEHLDDSNWRFLDCRFVLTEPEQKQAEFALSHL
                     PGATYAHVNRDLAVPHIPGKTGRHPLPEKERLIKLFSTWGIDNSVQVVVYDDTGGAHA
                     VRLWWMLRWLGHDAVAVMDGGWPRWIQEGRQVTAALTVSEPKKFKASPRDNWLVTAED
                     IVADLDNYEVRILDARNSDRFQGRNETLDPVAGHIPGAVSAPFVENLDEDGNWKSKSV
                     LRLMYEKLLSGSPAEQAVSYCGSGITACHNILAMYHAGLGDSRLYCGSWSDWIADPKR
                     PVA"
BASE COUNT          891 a          674 c          681 g         1001 t
ORIGIN      
        1 agtatgattc tgtaacgtaa gaatctgcag gaaaaattcg atagctaacc tcaagacctg
       61 aacctgtcaa tccggcatag aaacaataat attaatgtgg tttgagtaac gggatgtgtt
      121 caattgtgtt gatcaatatt atgaaactgg tgaaagaatg atgttgacct attagtaatg
      181 tagatataga aatattcttt tcaaccatct gtttttatat aatttttatt caagaatagt
      241 attttttgtg agattttact tcttgacaaa atacaaatat tcaccgaaag taggtgaaaa
      301 tggttttgtc accgttaatg tagatatagt ttttctgatt ttcatagttt tgtctggaag
      361 cttaattttt tcagtccact gagtccgcat tttttgccgg tggccggtat gcatttccca
      421 caattatatg acttcacaat ttgagccagc ttttccgata ttcaggaatt gattccataa
      481 ttctcgctga aatccgcaag aaactactag agcactaaga tgtattggga aaaaatatgg
      541 caacttcgtt ttttcagggc agtcacgatt accatattct attttatgct gtttttggga
      601 acggcccagg ctcaggaaaa agagatgtcc gcggaaacgg aagcgcaatc cgaaaaaagt
      661 ccgtcctcgg aatatacctg gcattatgct gccgtttccg tcaccctcgt ttcggctttg
      721 atgtcctata atgccgccaa gtcctacaac gatctctcag caaaaaacag cacgctggca
      781 acacaatatg caaactccag cagcagtagt gaacaagcta gttacaaaag tgaatatgac
      841 agcaacgcca gtaaaatgaa atcctataaa agcagcatgc aaacttggga tattttaact
      901 ttgctcggtt taggctggga agcctatttg ctgaggagtg atgaatccgg ttccagtgct
      961 gtgaattccg gaagtattca atttccattc attccccggt tggcatatca aacatatcct
     1021 tacgggacaa aaacggtttt acgctggaac atgagtttct aatacacggg actttatgat
     1081 ttaaatcagt catatcagct ggtctaaccg acttttatat agtgaaatac aatacctccg
     1141 ggaataaaca atggatccgg aatttgggaa cttcgatcag cttaaacata aagctcgttc
     1201 caaatctctt gatgcgtcaa actttttccc tgccgaattt cttttctgcg tagagaaatt
     1261 tatttttgca atgtactgtg tttagattgg ctgagcaaaa gactcaaaaa gtaagaaata
     1321 attacaggaa atcttaagta ctgaaatact atttttttgt taaattacct tcatgccacg
     1381 acaaccaacc cgtaacgact acattgtgct gtttgccctt ggtgccttgt ggggttcttc
     1441 ctttggcgca attaaaattg ctctgcacgg agttactcct ctgacagtaa tgagtgtgag
     1501 aattttgctg gccggtgcag ctttgttgct acttattcga gtacgtaaaa ctcctttccc
     1561 acgtggaata caaaactgga tcaaaattgg ctggatggct cttttcggaa cattgatccc
     1621 tttttttctt gtaccctggg ggcaactgca gattgacagc agtctggccg caatactgat
     1681 ggccgtcaac cctttattcg cacttacact gggacatttt tttagcgacc atgagagttt
     1741 tactttacgc caattactgg caatgctggt tggattttct ggtattttgc tggtattcgg
     1801 tgaaaatgct atttcctcaa taaatggaaa tatctgggca caattagcgg taatcggtgc
     1861 cggattctgt tatactattt ccggagtaat tgtatctcgt gttcgtggtg cttcagccga
     1921 ctcggtcagc gcctcaattt ttatctgctc atctgtgatc gtgtttccgc tgtggatgat
     1981 tcttgaacag ccatggagcc tccactttga aacagaaagt ctgttggccc tgactcacct
     2041 gggcctcgtt tcaaccggaa tggctttttt gatgcgctac tacatcatac tcagagcagg
     2101 tgctgtcttt ttgtcctatg tggcctttat aattcccatg ttcggaatat tgtttggaat
     2161 attgttttta ggcgaaacta tttctgtaaa taccatgggc gcagttgtac tgattctttc
     2221 tggtgtttat ttgggacgca agtaataagc ggatctttag atcaacaaac gcataagttt
     2281 ccaccatcct tctattaaga gattaacatg tacagttcac taatcactcc caaaattgca
     2341 aacgaacatc ttgacgactc aaactggcgt tttctcgatt gccgctttgt attaacagaa
     2401 ccagagcaaa aacaagccga gtttgctctt tctcatcttc ccggtgctac atatgcacat
     2461 gtcaaccgtg atcttgccgt acctcatata cccggaaaaa ctggacgaca tcctttaccg
     2521 gaaaaagaga ggctaataaa attattttca acttggggca ttgataattc tgttcaagtt
     2581 gttgtttatg acgatacagg aggagcacac gcggttcggc tctggtggat gttgcgctgg
     2641 ctcggacatg atgctgttgc tgtgatggat ggtggatggc ccagatggat tcaggaaggc
     2701 cgacaagtaa ccgcagcttt aactgtttcg gaaccaaaaa aattcaaagc ttctccgcgg
     2761 gataattggt tagtcacagc tgaagatatc gtagcagatt tagataatta tgaagtccga
     2821 atccttgatg caagaaactc tgacagattc caaggtagaa acgaaaccct ggacccagtt
     2881 gccggacata ttccgggggc tgtatctgca ccatttgtcg agaatttgga tgaagatgga
     2941 aactggaaat ctaaatccgt actccgcctg atgtatgaaa agttactttc cggttctccg
     3001 gcagaacaag ccgtttctta ttgcggttcc ggaatcaccg cctgtcataa catccttgcc
     3061 atgtaccatg ccggtctggg agactcccgt ctctattgcg gttcctggag tgactggatc
     3121 gccgatccca aacgcccagt agcttaattc agagctgcat agaacaaact cagctcttaa
     3181 gtcaatctta gcaaatttgc gtaagtaaga ttacgaattg tcagattcct ttctgaagtt
     3241 tatcctt
//
LOCUS       DTVD01000019            3227 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11681_length_3227_cov_257.840479, whole genome
            shotgun sequence.
ACCESSION   DTVD01000019 DTVD01000000
VERSION     DTVD01000019.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11681_length_3227_cov_257.840479"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(42..1544)
                     /locus_tag="EYO68_00595"
     CDS             complement(42..1544)
                     /locus_tag="EYO68_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06335.1"
                     /translation="MRENGVIISVDENKIQLLTLEGDIRNIPRFDIIYIAYYPIGNIR
                     IPKNVAAKDSMVITIKTLYEGEVFDLVKGWMIDHSEDQFSFLSTTGTPTVVDTEDIWD
                     IEITPLSQSINSEPSLSSPYEFVHPYPFMECKTGTEGIGAANELKPRIYPQYLLSEPL
                     LIKKELDRLKKGYDRLQKYDENKRFYPVPKLFSNDTSLGMWVNLGSRYGSSRNRNNSF
                     IPFIRSELTEGPFGFQRVLVTGVAPMNFGIHEEPQFQASYQMKSSYVHFSIMVDISRF
                     NIGQEKYKWSPDEMESYDDRENEIHSISGGFDYGEWSVDVSIMNSVYYAIKHDSLFHK
                     DDIEMSKNGIFYHNRFFRIELFQGFGIDKKPEPVPLDGAKNKFEEAAIEAYNESLLSI
                     QNFNTKLNFYRLNLELYNFKFFSPTYSLIQKSLQFKRENAPGESQGEFRYSSKSLTNA
                     LFLDYSLDAELKLRGYFSLESSTRKYSESGSLETSSKAYPKAGLSVAFLF"
BASE COUNT          979 a          605 c          642 g         1001 t
ORIGIN      
        1 gtggttgatt atttcactgc gcccactagg atcgtcttaa ttcaaaaaag aaaggccaca
       61 gacaaaccgg ccttaggata ggctttggat gaagtttcca ggctaccgga ttcagaatat
      121 tttcgagtac tcgattccag tgaaaaatat cctcttagct tgagttctgc gtccagggag
      181 tagtccaaga agagcgcatt ggtcagggac ttactggaat aacggaattc tccttgactc
      241 tctcccggtg cattctcccg tttgaattga agtgattttt gaatgagaga ataagttggt
      301 gaaaaaaact taaaattata taattcaaga tttaatctat aaaaatttaa tttagtatta
      361 aaattctgaa ttgaaagaag actttcgtta taagcttcaa tcgcagcttc ttcaaacttg
      421 ttttttgccc cgtccaatgg aactggttct ggttttttat caatgccaaa tccctgaaat
      481 aattctatcc ggaaaaaacg gttatggtaa aaaattccat tcttgctcat ttcaatatca
      541 tccttatgga atagggaatc atgcttgatg gcataataga cagaattcat aattgagaca
      601 tctacagacc attctccgta atcaaaacct cctgaaattg agtggatctc gttttcccga
      661 tcatcataag attccatctc atcaggagac catttgtatt tttcctgccc gatattaaaa
      721 cgtgaaatat ccaccatgat ggaaaaatga acatagcttg atttcatttg gtaggaagcc
      781 tgaaactgtg gttcttcgtg gattccgaaa ttcatcgggg caactccggt aacgagtacc
      841 cgttgaaatc cgaagggtcc ctctgtcagc tcactcctga tgaagggaat aaaactattg
      901 tttctatttc tggatgaacc ataacggctg cccaggttga cccacattcc aagagatgtg
      961 tcattactga aaagctttgg gacaggataa aatcgtttat tttcatcata tttttgtagc
     1021 cggtcatagc ctttcttcaa tcggtccagc tctttcttga tcagaagagg ttccgatagc
     1081 aggtattgag gatatattct gggcttcaac tcattggcag ccccaatgcc ctctgttcct
     1141 gttttgcact ccatgaaagg gtagggatga acaaattcat atggtgatga aagtgatggt
     1201 tctgaattaa tactttggct cagaggagta atctcaatat cccagatatc ttccgtatct
     1261 actacagttg gcgtcccggt tgttgaaagg aaagagaatt gatcttcaga gtgatcaatc
     1321 atccagcctt ttaccaggtc aaacacttcc ccttcataca aagttttgat agtaataacc
     1381 attgaatctt ttgctgccac atttttagga atacggatat ttcctattgg atagtatgcg
     1441 atgtagatga tgtcgaaacg gggtatgttc cgtatgtctc cttccagggt caacaattga
     1501 atcttatttt catcaacact gatgataact ccattctccc tcatgcaggc agaaaaatag
     1561 actgaggaga tattgataca gtaaacctgc tgtccggtaa ttaataaaac agacagtaag
     1621 aaaaactttt tgcagatgct agagaaaaaa ttcaatcgcg actgcaaatg aagctttttt
     1681 attagaagat tcattattat cgttagctgc ttgggaatgg tagtttctga tgaaatagtt
     1741 aaggttgtat ttgagagaaa cgtaatcttc aaagacttgt ttgagtttca gggatgtaat
     1801 cagttgatcg aatttcaact gatagttttc tccggataat ctttcgtcgt actggcccgc
     1861 gaaatatact tcactgactc caaaatgaat gtcttcgaat aattcatgaa cataatttag
     1921 tctcaggtac tgggaattca aatcaaatga ttccaactgg tttatcaaat ctgtggattg
     1981 ctgcgtgata ctgacttcgt tgaccatttc ttcagctcta atcagtttaa tattggtttc
     2041 agatggatgg ctcgatttga gtctcatcga agaaagtatg acctgggctt ctgttctttc
     2101 agttgtgtat ttaatactgg caactggttt tgactcctca gaaagaagtt cacggaatat
     2161 attgttgccc tgaatggcaa tgattggata atataatcct ccagaaactg aataacttcc
     2221 cacgtcaaag cctgttattg caatttgatt gaactgggat agagccagtg cttcctgggg
     2281 atcatatgtt gaaaaataat cattaaatac agcccttcgc ttgatgatgt aatcgctgcc
     2341 ataggaaaag gcccaaagat ttgcagcaaa agaaagagac aggaaatgat atttcccaga
     2401 gaattgtgca gccaacactg gttcaacatt cggaagcaga gcaacatcac tggatcctaa
     2461 agtcactgtc ccctgaggcc caaaatttct gccggttgac cattgaaagt tcagagggag
     2521 tcccatcggg atttcagcct tgtaatcatc tctttccata atgaaaccaa ttttagtttg
     2581 gcggtcataa agatatggtc gagcataaaa ataggttctg ctttgaaaac ggttcaactg
     2641 atggaagcct ttctgaaaaa ccgaaagaaa ttttgaaata ctgattttat cgctcagcat
     2701 tctggttggt tgaacggtgt cagtagtctt ttccctggta atttgacatt ccggcatatt
     2761 gctgccaaaa ccgaattcgt agggtgtatg ctgtagcaat attccagccg tggaaatgat
     2821 agattcaact gattcaaaac ctttcaactg gtcaatattg accagatgtg ttttaccgtt
     2881 tatgtcgtaa aaaacgatca aatcttcaaa aaaccgaata ggccagccaa tgaaactgaa
     2941 atccggtttt ccctgaactg ttacttttct tggaggaact tttagattgt ttagaaagtc
     3001 aattcgtggg attggatttt ctacggtgtt gaaaataaga acactttcta tttctttttt
     3061 tggcaggact cgaaaagaac cattcttatc aagtaattga atttcattct catcaacatc
     3121 aaagatcagg ccagtatagg agagacattt tttatagata atagagtaaa tctctgaagc
     3181 atgaagggtt gactcaaaga aaattattaa aacaaaagtg cacagaa
//
LOCUS       DTVD01000020           12394 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1169_length_12394_cov_286.199773, whole genome
            shotgun sequence.
ACCESSION   DTVD01000020 DTVD01000000
VERSION     DTVD01000020.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1169_length_12394_cov_286.199773"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            119..502
                     /locus_tag="EYO68_00600"
     CDS             119..502
                     /locus_tag="EYO68_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06336.1"
                     /translation="MVGVAWTFDTGNVVVPTSGSTYWYNMTSDNLSNACSSTYTGTSS
                     IPVILFTRSEAPSVGETELGSGNTVTFYDGRISYGIWTGKIKIDTVTTTAVTGKMYAK
                     GSDSDNEINGTFTLSRCCSGSLCSE"
     gene            complement(594..4193)
                     /locus_tag="EYO68_00605"
     CDS             complement(594..4193)
                     /locus_tag="EYO68_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06337.1"
                     /translation="MQFETRLWSFTQGVRGRIAISVLIGMIAAALGVIRLALLGWLIG
                     LIFLGQSADELVMPIIFAALVMLMRGIFEHWRTMTAHETSAIVQKQLRRRLFDKIVSL
                     GPGYTGRQRSGELVLTLVDGIEQLETYFGEYLPQLFISALTPLMIFAFVAFLDFPVAL
                     ILLVAAFVSLAAPSAWHKFDTNRSLERQQAYAAFASDFLDGIQGLATLKSFGQSGSRT
                     ELLKKRSFELFRSTMWVLATNSLSRGITDTSIAVGAAAGLGYGAYRTMAGEMELTTLL
                     IILMMGVEIFRPLRDLRTVLHQGMVGLSAAQGVYKILDAQPVVLEEHSGNSTTRLEPS
                     IDFDSVSFSYPEHSHIVHNCLNFNISKGERVGIVGSSGCGKSSIVKLLLRFHDPGQGS
                     ISIGGKDLRNITLDQLRREISVVHQDTFLFHGTIEENIRMGNPEASETEITDAAKNAN
                     IHEFVTTLPDRYKTIIGEKGIKLSGGQRQRIAIARALLRDTPILILDEALSAVDAENE
                     YVIQQALDRLMKNRTTLVLAHRLSSIIKCDRILVMDQGRVVESGHHEALMKKDGIYSA
                     LMNEQVREAELKELVNGEEESMFVNNGTVEQIDESLRDLPTEGIIKAEGLNWKQLIVE
                     LMRHIMPWKGRLLLTFCFGVLRVLAFIGVGIFSALIVLALKNGTDFTFQLTGLMVVAS
                     LSGILHWIESWVAHDMAFRLLAEMRINVFRKLDKLAPAYLVRRRTGDLMGIATQDVEL
                     VEYFFAHTVAPVFVSILIPVAVITFMTTVSFWMALALLPFLLAVGFSPLLMRGKVDRL
                     GSKAREAAGELSAHAIDTIQGIAEIVAFQQGFNRGKVFEELTNRHISLRLPFFRELTL
                     QTTLLEVLTGWGGLAVVCTGALLTANGSFDAGLLPLLTILAMSAFLPVSEIAQIGRQL
                     ADTLGATRRVYALENEPVVIADGDGEASSDLSSGFSFQNVSFSYPGRSIRVLQNLNFE
                     IPVGKTLALVGPSGAGKTSLAQLLMRFWDPDKGSISLNGIELRDYKLEDLRSRIALVA
                     QDTYLFNNSLRENILVAKPDATELELSEAVKLSALSEVVESMPDGLETIVGERGASLS
                     GGQRQRVAIARAFLKDAPFLILDEATSHLDALSEHTVHKALNLLKTNRTTIIIAHRLS
                     TIRDADRIIVLNEGRMVETGTHKELLQKTGLYRQLVSKQLSGSGLKDHKVANF"
     gene            complement(4476..4952)
                     /locus_tag="EYO68_00610"
     CDS             complement(4476..4952)
                     /locus_tag="EYO68_00610"
                     /inference="COORDINATES: protein motif:HMM:PF03994.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HIC06338.1"
                     /translation="MVISHFINVSIEILIYFLVSILCLFIGRKVLDWITPYDLNNQTS
                     IEKNLAAGITEAGFYIAMAIIVHASVSGVVDYDMFSFIDSENPSRYSLLGAELITTAI
                     YLLLGLICLSLGRRSLDWVTPFDLNKEIETERNVGVGAIEASIYISIAIIIHGIIV"
     gene            5465..7237
                     /gene="xsc"
                     /locus_tag="EYO68_00615"
     CDS             5465..7237
                     /gene="xsc"
                     /locus_tag="EYO68_00615"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIC06339.1"
                     /translation="MKMTTEEAFIKVLQKHGIEHAFGIIGSAFMPISDYFPQAGITFW
                     DVAHECNGGYMADGFTRTTGKMSMIIGQNGPGITNFVTCVKTAYWNHTPMLLVTPQAA
                     NKSIGQGGFQEVEQMAGFKDMVAYQEEVRDPTRIAEVLNRVITKARRMSAPAQINIPR
                     DIWTQVVDIELPEIIEFELPAGGTDAISSAAKLLSDAKFPVILNGAGVILGNAIPASQ
                     ALAERLDSPVCCNYQHNDAFPGSHPLFVGPLGYNGSKAAMELIKEADVVLALGTRLNP
                     FSTLPGYNLDYWPNEAKLIQVDINPDRIGLTKPVSVGIVGDARQVAEQILSRLAEKAG
                     DAGREERRKLIERKKAVWAETLYSMDFEQDDPGTTWNERVRARQPDHMSPRMAWRAIM
                     KVLPEEAIISSDIGNNCAIGNAYPTFENGRKYLAPGLFGPCGYGFPSIIGAKIGNPET
                     PVVGFAGDGAFGISMNEMTAIGRSEWPGITMIIFRNYQWGAEKRNSTLWYDDNFIGTE
                     LDKQVSFAEIANACGVKGVMVKTMDELTSTLASAVKAQMEKGETTFIEAVVDQELGDP
                     FRRDAMKTPVPVAGISKDDMCAQK"
     gene            7351..8703
                     /locus_tag="EYO68_00620"
     CDS             7351..8703
                     /locus_tag="EYO68_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC06340.1"
                     /translation="METQNMNAPDSLWRPMTQHKMLLQNHPKHIVKAEGCFMINEDGD
                     RILDGTAGLWCVNVGHGRKELAEVAKAQMEKLAYVIPVMTSDPAVELSAKMLEKLGME
                     KGHVYFTSSGSEANETAFKMVRQYHLQTGNPRKHKIISRHRAYHGNTLATMSATGQAE
                     RKIGYEPLAPGFLHIPPPYPYRRHPKLTEEEHGTECVKFLEDTIIYEGEETVAAFIME
                     PMISGGGVMVPPDNYLTEIRKVCDKYNVLLILDEVVSGFGRTGKMFGHEHWGAKGDIF
                     TFAKGMASGYMPVAATVVKENIFEAFYGEANELKHFRHVNTYGGHPVAAAVSLKNIEI
                     VERENLVANAAKMERYIKAGLNEFADHPFVGEIRGKGLLLGIELVSDKESKTPLETAR
                     SNAIVQNCMKQGVHVMRNGYTVPGLDNVLIMAPPLVLQESEADMIVDAIGNALKQALN
                     "
     gene            8813..9925
                     /gene="ald"
                     /locus_tag="EYO68_00625"
     CDS             8813..9925
                     /gene="ald"
                     /locus_tag="EYO68_00625"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIC06341.1"
                     /translation="MRIGVPKEIKEMENRVGATPAGVAELVRNGHQVFVEKDSGLGSG
                     FSNSEYQEAGANMVDTAAEVWSTEMVYKVKEPLPEEYGFFREDLLLFTYLHLAAEEAL
                     TNALMSSKTTSIAYETVQLPDRSLPLLTPMSEVAGRMAVQVGARFLEKPQGGQGVLLG
                     GVPGVEPAEVVIIGGGVVGINSAKMAVGMGAKVTILDVSADRMRYLDDVFNGRVQLVM
                     SNSYNIARAVESADLLVGGVLIPGKKAPHLVTEAMVKTMKQGSVIVDVAIDQGGCIET
                     IDRVTYHSDPVYVQHGVVHYSVGNMPGAVARTSTLALTNVTTPYAVQLANRGWKTACK
                     EDHALALGINTHAGNVICKGVSEAFGYEFVPPEQIL"
     gene            complement(9999..10967)
                     /gene="speB"
                     /locus_tag="EYO68_00630"
     CDS             complement(9999..10967)
                     /gene="speB"
                     /locus_tag="EYO68_00630"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIC06342.1"
                     /translation="MKNEENTPIQPVSGKIVPRYAGPATFARLPELRDVKKCDIAILG
                     IPFDSGTSYRPGARFGPQAIRQASRHLRTQYHPAYDTEPFAELQVADAGDIACNPFNI
                     EEAVGQVQTAATDLLGKVGSIISLGGDHTIALPLLRAVNHFHGSVALVHFDAHLDTWD
                     TYFGAPYTHGTPFRRAAEEKLFLDHASIHVGIRGPLYSRDDLTNDEELGFKVVHCDEF
                     QNEGVEHVIKRIKDRVGNNPMYLSIDIDVLDPAHAPGTGTPEIAGMSSRELLGVLRGL
                     AGLNIISADVVEVAPAYDHAELTSLAAATTVFELINLLAHYRKNNT"
     gene            complement(11076..12095)
                     /locus_tag="EYO68_00635"
     CDS             complement(11076..12095)
                     /locus_tag="EYO68_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06343.1"
                     /translation="MLSPITLRRLQHFRANRRGYWSLWIFLSIFFVCIFAEVVANDKP
                     LLVIFEGHLIFPVIETIPETEFGGEFATEAEYRDEFVRELIEKKGEIYWPMIRYSYDT
                     INYDLPAAAPSHPTGENWFGTDDQGRDVVARLIYGFRISVLFGLVLTILSSIVGVAAG
                     AVQGYFGGRVDILGQRFIEIWSGLPVLFLLIILSSFVEPNFWWLLGIMLLFSWMGLVG
                     VVRAEFLRGRNLEYVRAARALGMSHSRIMKRHILPNAMVATMTFMPFILSGSITTLTA
                     LDFLGFGLPPGSPSLGELLAQGKANLHAPWLGIAGFSVIALQLSLLIFIGEGVRDAFD
                     PRHNA"
     gene            complement(12095..>12394)
                     /locus_tag="EYO68_00640"
     CDS             complement(12095..>12394)
                     /locus_tag="EYO68_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC06344.1"
                     /translation="ITERRVLYKHVFRNAMLIIIAGFPAAFIGMFFTGSLLIEIIFSL
                     DGLGLLGFESTIQRDYPVMFGTLFVFTLLGLIVGIISDLMYTVIDPRIDFETREA"
BASE COUNT         3649 a         2673 c         2790 g         3282 t
ORIGIN      
        1 attttatttt attatcatcc tgatcttcgg tgcatgttca gtaacaaccg aagagaaagc
       61 cactgccacc agctcgacaa ctataccgga ctatgaaacc actactttgt caggaaaaat
      121 ggtaggggta gcatggactt tcgatacggg gaatgtggta gtacccactt ctggctccac
      181 ctattggtat aatatgacaa gcgacaatct cagtaacgcc tgttcctcta cttatactgg
      241 aacttccagc attccagtaa tattatttac tagaagtgaa gcaccttcgg ttggtgaaac
      301 agagcttggt tcgggaaata cagtgacgtt ttatgatggc agaatcagct atggtatttg
      361 gacaggtaaa atcaagatcg atacggttac tactacagca gtgactggaa aaatgtatgc
      421 gaaaggaagc gacagtgaca acgaaatcaa cggcaccttc actcttagcc gctgctgctc
      481 aggttcgctt tgctctgagt gatattatta caattacgga gtttgccagc agaaggtgtt
      541 acgtaacaat tcagcataaa aaatattgag tttcgaaagc tgattatttt ttgtcagaag
      601 ttggccacct tgtgatcctt caaacctgat ccgctgagct gtttggaaac aagttgtcta
      661 taaagaccgg ttttctgcaa caactcttta tgtgtaccgg tttccaccat tcttccctca
      721 ttgaggacta ttatccggtc cgcgtcacgg atggtagaaa gccgatgtgc tatgataatg
      781 gtggttcggt ttgtcttaag cagattaaga gctttgtgga cagtgtgttc gctgagagcg
      841 tcgagatgag aagtggcctc gtctaaaatt aaaaacggag catcctttag gaatgctctg
      901 gcaattgcaa cacgctgtct ttgacctcca gaaaggctgg cgccacgttc accaactata
      961 gtttccaggc catccggcat actttctaca acttcagaca gtgcagacag tttcactgcc
     1021 tcacttaatt ccagctcagt agcatccggt ttagcaacga ggatgttttc ccgcagtgaa
     1081 ttattgaaga gatatgtgtc ctgagcaacc aatgcgatcc gggaacgcaa atcctccaat
     1141 ttataatctc tgagttcaat tccattcaat gagatggaac ccttatctgg atcccagaaa
     1201 cgcattagca gttgagcgag gcttgttttt ccagcccctg atgggccaac taacgcaagc
     1261 gtttttccta caggtatttc gaaattgagg ttctgcagga cacgaattga tcttcccgga
     1321 tatgaaaaac tgacattttg aaaggaaaaa cctgatgaca aatcgctgga tgcttcacca
     1381 tctccatcgg caatcaccac cggttcgttt tccaatgcgt aaaccctcct cgtggcaccc
     1441 agtgtgtcgg ccaattgtcg tcctatctgc gcgatttcgg ataccggtaa aaatgccgac
     1501 atagcgagaa tagtaagcag aggaagcaac cccgcatcaa acgaaccatt cgctgtgagc
     1561 agagctcccg tgcagacaac agcaagacct ccccagccgg tcaggacttc aagcagggtt
     1621 gtttgcagtg tcaactctct gaagaacggc aaccttaacg agatatgccg gttcgtaagt
     1681 tcttcaaaaa cttttcctcg attaaaaccc tgctgaaatg caactatttc tgcaataccc
     1741 tggattgtat caattgcatg ggcagaaagc tctccggcag cttctctggc ttttgatccc
     1801 agtcgatcta ctttgccacg cattagtaaa ggactgaagc ctactgcaag cagaaatggc
     1861 aatagcgcca atgccatcca gaagcttact gttgtcatga acgttatcac tgcaactgga
     1921 ataagaattg aaacaaaaac cggagctact gtatgtgcaa agaagtattc caccagttca
     1981 acatcctgtg tggcgattcc cattaaatct ccagtcctgc gccgaaccag atatgcggga
     2041 gcaagtttat ccagtttcct gaaaacattt atcctcatct cagccagtaa cctgaaggcc
     2101 atgtcatggg ccacccatga ctcaatccag tgaagtattc cggaaagtga tgcaactacc
     2161 attagtcctg tgagttgaaa agtaaaatca gtaccgtttt tcagagcaag aacaattagc
     2221 gcgctgaaga tccccacccc aataaaagca agcacccgca gtacgccgaa acagaaggta
     2281 agtaaaagcc tgcccttcca gggcatgatg tggcgcatta attcaacaat taactgcttc
     2341 cagttgagcc cctctgcttt aataatgccc tctgtcggca agtcacgtaa agattcatct
     2401 atctgttcta cagtcccgtt atttacaaac attgattctt cctcaccatt taccaattct
     2461 tttaattctg cttcgcgaac ctgctcattc atcaaagccg aataaatacc gtctttcttc
     2521 atcagggctt catgatgtcc agactcaaca acccttccct ggtccattac cagtatccgg
     2581 tcacatttga ttatacttga aagccgatgg gccaaaacga gtgtggtgcg gtttttcatc
     2641 aaacgatcaa gggcctgctg gatgacgtat tcgttttctg catcaacagc agacaatgcc
     2701 tcatctaaaa ttaaaatggg cgtatccctc aaaagagccc ttgcaatagc aatccgctgc
     2761 cgctgcccac ctgaaagctt gatgcccttt tcaccaataa tcgttttgta tctgtccgga
     2821 agagttgtta caaactcatg gatgttagca ttttttgctg catcggtaat ctcagtttcg
     2881 cttgcttccg gattccccat tcgtatattc tcctcaattg ttccatgaaa aaggaatgtg
     2941 tcctgatgaa caaccgaaat ttctcttcgt aactggtcga gggtgatgtt tctcaagtcc
     3001 tttccaccaa tgctgatgga tccctgccca ggatcatgga aacgtagtag aagcttaacg
     3061 atcgaagact tgccgcaacc acttgaaccg acaataccta ctctttctcc cttgctgatg
     3121 ttgaaattca gacaattatg gacaatgtgg ctgtgttccg ggtaactgaa gcttactgaa
     3181 tcgaaatcaa ttgaaggttc aagacgggta gtgctgtttc cggaatgctc ctccaataca
     3241 accggttgag catcgagaat tttgtatact ccttgtgctg cagaaagacc taccataccc
     3301 tgatgcagaa ctgtacgcag atcacgcaga ggcctgaaaa tttcaactcc catcattaaa
     3361 attataagca atgtggtgag ttccatttca cctgccatcg tacggtaggc tccatagccc
     3421 agtcccgctg cagcaccgac tgcgatagat gtatctgtga ttcctcttga taaagaattg
     3481 gtggcaagaa cccacatggt actgcgaaaa agctcaaaag atcgtttttt cagcaattct
     3541 gtacgtgatc cgctttgacc aaaggatttg agtgtagcaa ggccttggat tccgtctaaa
     3601 aaatctgatg caaatgctgc atatgcctgc tgcctttcaa gtgatctgtt ggtgtcaaac
     3661 ttgtgccatg ctgatggtgc tgccagtgac acaaaagcgg ctaccagcag gataagtgca
     3721 accggaaagt cgagaaaggc aacaaaagca aatatcatca gtggtgtcag tgcagagata
     3781 aacaattgtg gtaggtattc tccaaaataa gtctccagct gttctattcc gtccactaat
     3841 gtcaatacga gttcgccgga tctttgtctg ccagtatatc caggtccaag gctcactatt
     3901 ttgtcaaaaa gacgacgacg cagttgcttc tgaacaattg ccgatgtctc atgcgctgtc
     3961 attgttcgcc aatgttcaaa aatacctcgc atcagcataa ctaaagcagc aaaaatgatt
     4021 ggcataacta attcgtctgc agactgacct agaaaaataa gaccgatcag ccaaccgagc
     4081 aaagcaagac gaatcactcc gagtgctgct gcaatcattc cgattagtac tgagatggca
     4141 atgcgacctc taacaccctg ggtgaatgac caaagtcgtg tttcaaactg cattaattaa
     4201 tctgggaaaa tattattgag ttgattttct acaagaaatt ccggcaggca actccaaaca
     4261 aatttaactc gtccaagagt tgacggtcac gcaaaaagtc taaagtacac tatttgtcat
     4321 ttcgaccgta gaaaaatctt aatgatatta gtataatagt ctatgcggga tttcttgctt
     4381 tgctcaaaat gtcacgtttt tagggctttt acaacggcat caagagttgg tctgataata
     4441 ttagcttatc aacaaaacga gatgctaagg ttggatcaga ctataatgcc gtgaatgatg
     4501 attgctatgg atatgtagat tgaagcttct attgcaccaa ctccgacatt gcgttctgtt
     4561 tctatttcct tatttaagtc gaaaggagtg acccagtcca gtgaacggcg tcctaaactc
     4621 aggcaaatta agccaagcag caaatagatg gctgtggtta tcaactcagc gccaagcaac
     4681 gaataacgag acggattctc tgaatcgatg aaggaaaaca tatcgtagtc aacaactccg
     4741 gaaacagaag cgtggacgat aattgccata gcaatgtaga atcctgcttc agtaatacct
     4801 gcagcgagat ttttctcaat cgaagtctgg ttgttcaaat cataaggtgt aatccaatcc
     4861 agtactttcc tgccgatgaa caaacacaag atacttacca ggaaatagat caggatttca
     4921 attgaaacat ttatgaaatg actgattacc ataatcaact cctaaaaaaa gtttagataa
     4981 taatatttta caaagttatt taaccgtaat aaaccgcaat gcttcatttt caaactgtca
     5041 actaagttgc acagtcaatt ctactaattt aattattatt cgcaagtcaa aatactgtga
     5101 gcaaaattac atatttttat aatgcctttt ttgaacattg tcaaatattg atttaccaga
     5161 gataaaataa gttgatttaa tttatgttat gaagtataaa tttactatga attctgttta
     5221 gcgagacttc aggtctcact gaatgaaata ttttccggat ttaatgcaaa agtactgttt
     5281 ataaattgct gtctgggtag agtcctacag cagattttac tgtaaattga cttaaagacg
     5341 gaatatattg gtgaattcgg attttatgat aaatctacta gacttatttt gcggaaagtt
     5401 ggattttata taatggattc cgataaccgc ttttttgaaa cacaacatta atggaactca
     5461 gcttatgaaa atgacaacag aagaggcttt cataaaagtc ctgcaaaaac acggtatcga
     5521 acatgctttc ggcataattg gttcggcttt tatgccgata tcagattact ttcctcaagc
     5581 tggcattacg ttttgggacg tagcacacga gtgtaacggg ggttatatgg ctgacggttt
     5641 tactcgcaca acaggtaaaa tgtcgatgat aattggacag aatggacctg gaataaccaa
     5701 ttttgtaacc tgtgtcaaaa ccgcatattg gaatcacaca ccgatgttgt tggttacccc
     5761 acaggcggcc aataaatcga ttggacaggg tggttttcag gaagttgaac aaatggccgg
     5821 tttcaaagac atggtggcct atcaggaaga agttcgtgat ccaacgcgta ttgcggaagt
     5881 actgaatcgt gttatcacta aggccaggcg catgtctgct ccggcacaga tcaatatacc
     5941 acgagatatc tggacacagg tagtcgatat tgaactaccg gaaattattg agtttgaact
     6001 tccagcaggc ggtactgatg caatatccag tgctgctaag ctcttatctg atgcaaaatt
     6061 tccagtaatt ctaaatggtg caggtgtgat tttaggcaat gccattcctg cctcacaagc
     6121 acttgcagaa cgtttagatt cacctgtgtg ctgcaattat cagcacaatg atgcatttcc
     6181 cggttcacat cctttgtttg tcggaccact gggctataat ggttcaaagg cagcaatgga
     6241 gttgattaaa gaggcggatg tggttttggc tcttggtaca cgattgaatc cgttttctac
     6301 gcttccaggt tacaacttgg attactggcc gaatgaggca aaattgatcc aggttgacat
     6361 caatccggat cggatcggat taacaaagcc ggtttctgtc ggaattgtcg gtgatgccag
     6421 acaggtggca gaacaaattc tgagcaggtt ggctgaaaaa gctggtgatg caggacgtga
     6481 ggagaggcgc aaattgattg aaagaaaaaa agctgtctgg gcagaaacac tttactccat
     6541 ggattttgaa caggacgatc cggggactac atggaatgag cgtgttcgtg cgcgtcagcc
     6601 cgatcacatg tcaccaagaa tggcctggcg tgcaataatg aaagttctac ctgaagaggc
     6661 aataatttct tcagatatcg gcaacaactg tgctatcggg aatgcctatc cgacttttga
     6721 aaacggaaga aaatatttgg ctccggggct ttttggacca tgtggttatg gtttcccttc
     6781 tataattggc gcaaaaatcg gtaatccgga aacacctgtt gttggatttg ctggagacgg
     6841 ggccttcgga atttcaatga atgaaatgac tgcaattgga cgcagtgaat ggcccggtat
     6901 cacgatgatc atcttcagaa attaccagtg gggtgctgaa aagcgtaact caacactctg
     6961 gtatgatgac aatttcattg gaacagagct tgataagcaa gtctctttcg cagaaatagc
     7021 aaatgcatgc ggtgtcaaag gagttatggt taagacgatg gatgagttga cgagtacttt
     7081 ggcttccgca gtcaaggcgc aaatggaaaa aggggaaaca acattcattg aagcagttgt
     7141 tgatcaggag cttggagacc ccttccgccg agatgccatg aaaacaccgg tgcctgtggc
     7201 aggaatcagt aaagatgata tgtgtgcaca aaagtgaagc ttactttgtt agtttaaata
     7261 cagccgtaca ttaaaaaaaa tctgataaaa gtgtgtatag atttataata taattgcggc
     7321 taattaatta atgattagaa aagggaaaaa atggaaacac aaaacatgaa cgctccggac
     7381 tctctctggc gtccgatgac tcagcacaag atgctgttgc aaaatcatcc aaaacacatt
     7441 gtgaaagctg aaggctgctt catgatcaat gaagacggag accgtattct tgacggaacg
     7501 gcaggtttat ggtgtgtgaa tgttggtcat ggaagaaaag aactggctga agtggcgaag
     7561 gcacaaatgg aaaaactggc atacgtaatt ccggttatga ccagtgatcc tgctgtagaa
     7621 ctgtcagcaa agatgctgga gaagctgggc atggagaaag gtcacgtata tttcaccagc
     7681 agtggttcag aagccaatga aacagcgttt aaaatggtgc gtcagtatca tttgcaaacc
     7741 ggaaatccgc gcaagcataa aattatttcc cgtcatcgtg cttatcacgg aaacacactg
     7801 gcaactatga gtgctaccgg ccaggctgag cgcaagatcg gctatgagcc acttgctccc
     7861 ggatttttac atattcctcc gccttatccg tatcgacggc atccaaagtt gactgaagaa
     7921 gaacatggta cagaatgtgt caaattcctg gaagacacaa tcatttatga gggggaagaa
     7981 acggttgcgg catttataat ggaacctatg atttccggtg gtggagtgat ggttcctcct
     8041 gataattatt taactgaaat caggaaagtt tgtgataagt acaatgtact tctaatcctg
     8101 gacgaagtag tttccggatt cggcagaaca ggaaaaatgt ttggacacga acactggggg
     8161 gccaaagggg acatttttac ttttgcaaag ggtatggcca gcggctatat gccggttgct
     8221 gctacggtag ttaaggagaa tatctttgaa gcattttacg gtgaagcaaa cgagctcaag
     8281 cattttaggc atgttaacac atacggtggt catcctgttg ctgcggcagt cagtttaaaa
     8341 aatattgaaa ttgtcgagcg tgagaatctg gtagccaatg ctgcaaaaat ggagcgctat
     8401 atcaaggccg gattgaatga attcgcggac catccttttg taggtgaaat ccgtggcaag
     8461 ggcttgcttc tgggaataga actggtttcc gacaaggaaa gtaaaactcc tctggagacg
     8521 gcacgttcca atgcaatagt tcaaaactgt atgaaacagg gagtccatgt gatgcgtaac
     8581 ggttacactg ttccggggct ggataatgtt ttaatcatgg ctccacccct ggtactgcag
     8641 gaaagtgagg cagatatgat tgtggatgca atcggaaatg ccttaaagca ggcattaaac
     8701 taaataaatg gtcgtcctgt ttgaataaat atgcttttta caggaggtta ttattcagta
     8761 tttcaactca tagaaaaaac aattgaaaac aagtacttga gaaaaggata aaatgagaat
     8821 aggtgttccg aaagaaatta aggaaatgga aaatcgggta ggtgctacgc cggcaggagt
     8881 agctgaactc gtgcggaacg gccatcaggt gtttgtagaa aaagactcag gtttgggcag
     8941 tggtttcagc aattcagaat atcaggaagc cggtgcgaac atggttgata cagccgctga
     9001 ggtatggtct accgaaatgg tttacaaagt caaagaaccg cttcctgagg aatatggatt
     9061 ttttcgggaa gatttgcttt tgtttaccta tctgcatttg gccgccgaag aagcattgac
     9121 caatgcgctt atgagcagta agactacctc cattgcatac gaaacagtac agctaccgga
     9181 tcgcagtttg cctctgctca ctccaatgag tgaagtcgcc ggacgcatgg ccgtacaagt
     9241 cggtgcccgt tttctggaga agccgcaggg cggacagggc gtgttgctgg gcggtgttcc
     9301 gggagtagaa ccggcagagg tagtgattat cggcggcgga gtagtcggaa taaactcagc
     9361 caaaatggcc gtcgggatgg gtgcaaaagt taccattctt gatgtaagtg ctgacagaat
     9421 gcgctatctt gatgacgtgt tcaacggacg tgttcaactg gtgatgtcca acagctacaa
     9481 cattgccagg gccgtggaaa gtgcagattt gctggttgga ggtgtgttga ttccggggaa
     9541 aaaagcgcct catctggtaa ctgaagccat ggtcaaaacc atgaaacagg gatccgttat
     9601 tgttgatgtg gctatcgacc agggaggttg tattgaaacc attgatcgtg tcacctatca
     9661 cagtgatcca gtttatgtgc agcacggtgt agttcattac tctgtgggca acatgcccgg
     9721 tgctgtagca agaacctcta cgctggcctt gaccaacgtc actacaccat atgctgttca
     9781 attagccaat agaggctgga aaacggcgtg caaggaagat catgccctgg cattaggtat
     9841 aaacactcat gcaggaaatg ttatctgtaa gggcgtttct gaggcttttg gttatgagtt
     9901 cgttccaccg gaacaaatat tataagtata catgatataa acttatcttg taatttcagt
     9961 gaactaacat gtccactgaa ataaacacaa ttcagttttc aggtattatt tttgcggtaa
    10021 tgcgccaata aattaatgag ttcaaaaact gtggttgccg cagcaagtga agtcaactcg
    10081 gcgtggtcat aggccggagc aacttctaca acatccgcag agataatatt cagtccggct
    10141 aagcccctta aaaccccgag taattcccgc gaagacattc ccgcgatttc cggtgttcct
    10201 gttccaggag cgtgagctgg gtctagcaca tcaatatcaa ttgacaaata cattgggttg
    10261 ttccccaccc gatccttgat gcgcttgatg acgtgttcca caccttcatt ttggaactca
    10321 tcacaatgca caaccttgaa tcctaattct tcatcattag ttagatcatc cctgctatat
    10381 aaaggtcccc ggattccgac atgaattgaa gcatgatcta ggaacagttt ttcttcagca
    10441 gcccgtcgaa acggtgtacc gtgtgtataa ggagcgccaa agtaggtatc ccaggtgtcc
    10501 aggtgcgcgt caaaatgaac gagagcgaca gatccgtgaa aatgatttac cgcccgcagt
    10561 aaaggcaaag caatcgtatg gtcaccaccc agactgataa tgctgcctac ttttcccaaa
    10621 agatctgtcg cagcagtttg gacttgtccg actgcttcct caatattaaa cggattacaa
    10681 gctatgtcgc ctgcatctgc cacctgcagt tctgcaaacg gttcggtgtc ataggccgga
    10741 tgatactgag tccggaggtg ccgtgaagcc tgccggatgg cctgtggtcc aaatctggct
    10801 ccggggcgat agctggttcc tgaatcaaag ggaattccaa gtattgcaat atcgcacttc
    10861 tttacatctc gtaattccgg aagcctggca aaagtagcag ggcccgcgta acgcggaacg
    10921 atttttccgc tgacgggttg aatgggagta ttttcttcat tcttcatttt tttcctttca
    10981 gttaaaattc agcgggcact tacttgagtt gaaacaaagt tttattaatt actgattaaa
    11041 ttaggagtta ttttcaagag agttttattg atatattaag cattatgccg gggatcaaag
    11101 gcatcacgga caccttcgcc gataaaaatc agcaaactta gttgcagtgc aatcactgaa
    11161 aagcctgcaa ttcccagcca tggagcatga agattcgctt ttccctgggc cagtagttca
    11221 cccagagaag gagatcccgg aggtaaaccg aagcccagga aatcaagtgc agtgagtgtg
    11281 gtgattgaac cggaaaggat gaaaggcata aaagtcatag tggcaaccat ggcattcggc
    11341 agaatatggc gtttcatgat tctgctgtga ctcataccca gagcccgtgc tgcccgcacg
    11401 tattccagat tacgccccct taaaaactct gcccgcacga ctccaaccag tcccatccag
    11461 ctgaagagca gcatgatacc tagtagccac cagaaatttg gctctacgaa acttgatagg
    11521 ataatcaaca aaaacaggac tggcagtccg gaccagattt cgatgaaacg ctgtccaaga
    11581 atatctacac gaccaccaaa gtagccctga actgcacctg cggcaactcc aacaatcgaa
    11641 ctcaggattg tcagcaccag tccaaacaaa acagaaatac gaaaaccgta aatcagccga
    11701 gctaccacat cacgtccctg atcgtcagtt ccaaaccagt tttcgccagt aggatgagat
    11761 ggggctgcag caggtaaatc atagttgatc gtatcataac tgtagcgaat catgggccag
    11821 tatatttcac ctttcttttc aattaattct cgtacaaact catcgcgata ttccgcttcg
    11881 gtggcaaatt caccaccaaa ttctgtttcc ggaattgtct caattactgg aaaaatcaag
    11941 tgcccctcaa aaatcaccag cagtggtttg tcatttgcga caacttcggc aaaaatgcaa
    12001 acaaaaaaga tggacagaaa aatccacaaa gaccaatatc ctctgcggtt tgctctaaaa
    12061 tgctgtagac ggcgaagcgt tatgggactc agcattatgc ttctctggtt tcaaagtcaa
    12121 ttctgggatc tatcacagtg tacatcaaat cactgataat cccgacaatc agtcccagca
    12181 aagtaaaaac aaaaagtgtc ccgaacataa ctggataatc gcgttgaatg gttgattcaa
    12241 agcccagtaa acccagacca tcaagtgaaa aaatgatttc aatcaataat gaacctgtga
    12301 aaaacatacc gatgaaggca gcaggaaatc cggctatgat tatcagcatg gcattacgga
    12361 aaacatgttt gtaaagcacc cgcctttccg taat
//
LOCUS       DTVD01000021            3219 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_11719_length_3219_cov_160.624526, whole genome
            shotgun sequence.
ACCESSION   DTVD01000021 DTVD01000000
VERSION     DTVD01000021.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11719_length_3219_cov_160.624526"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            73..1326
                     /gene="phnA"
                     /locus_tag="EYO68_00645"
     CDS             73..1326
                     /gene="phnA"
                     /locus_tag="EYO68_00645"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HIC06345.1"
                     /translation="MVSDIIKLNGVEYAWPKRPVVVVCIDGGDPEYIEAGIKDGIIPN
                     IENFMKNGFYSVAQGSMPSFTCPNNMSIVTGCEPVVHGISGNYYLDRGTSKPVLMTGP
                     DLLRTRSIMSEFSRHGAKVVSITAKDKLRKQLQKDMNLSSGSVSMSSQYAHDCSMEEN
                     GIEKFLEFVGLPQPEMYSADLSLFVLQAGIKFLEERRPDILYLSLTDFIQHTHAPGTD
                     VANQFYKAMDALFGQMVELGAVVALIADHGMGDKADAKGDPKVIWLQDILNAELGEGI
                     CTVICPITDAFVGHHGALGGFVRVYINGRAEREKVVEIVQDQTGIEKAWLAEDVAEEL
                     EQPLDREGDVAVMGDKWTVIGGRAVDHDLSALKGHRLRTHGSLYEANIPFVISEPLTG
                     SYAERHLTTTLRSHEIFDYVLNGVS"
     gene            1522..2418
                     /locus_tag="EYO68_00650"
     CDS             1522..2418
                     /locus_tag="EYO68_00650"
                     /inference="COORDINATES: protein motif:HMM:PF03446.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC06346.1"
                     /translation="MNSITCAFIGLGKMGRELCGRIGGAGYRTLAYDLDPEASKYAEE
                     SYKNVTSSALLPAVKNSAVVFSCLPNSHHVSEVVNNLLQQEGTLNKVQYWVDTTSGHP
                     GESQKIASELMSQDVIFFDCAVSGGPAGATAGTLTAMVGGDHSAFGEVQSIISSFAKN
                     IVHLGPSGAGHAVKAVNNTLLAANICSVSEGLIALKKYGIPLDVALKAISTSSGRSWV
                     TQQRMPEHVLTRGFDYGFSLGLLCKDVDTCMGMLQESNIPAPSLRVTREIIQVAKNIL
                     GDESDHLEIVKIIEDWSGETIE"
     gene            2561..>3219
                     /locus_tag="EYO68_00655"
     CDS             2561..>3219
                     /locus_tag="EYO68_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC06347.1"
                     /translation="MKTPELGFSPKGLYIGGKWEESVKGKTFESINPANMDYLGELPL
                     ADEEDVNRAVIAAKEAFYDWSRMPIKERAGFLDALAEILQDKRDELGLMDCLDSGNTI
                     SGMKGDVDWSADTLRYFAGLIAEIKGETFSEKPGHLNFTRREPYGVVAKINPFNHPLR
                     FCAEKSAAALAAGNTVVIKASEQAPLSSLRFAEICHEVLPPGVVNVLTGDAACGQAMV
                     R"
BASE COUNT          933 a          584 c          756 g          946 t
ORIGIN      
        1 tattacaatt aataataaag ccctcctcga aataatatat tcttataatt caactacatt
       61 caatatctca ttatggtttc agatataata aagctgaacg gggtagaata cgcttggcct
      121 aaaaggccgg ttgttgttgt ttgcattgat ggtggcgatc ctgaatacat tgaagcgggc
      181 atcaaggatg gcattattcc taatattgag aacttcatga aaaatgggtt ttattctgtt
      241 gcacaagggt ctatgccgag ttttacttgt cccaacaata tgtcaattgt tactggatgt
      301 gagcctgttg tgcatgggat ttcgggcaac tattacctgg atcgtgggac ttccaaacct
      361 gtgttaatga ctggccctga tttgcttcgt actcgttcaa tcatgagtga gttttccaga
      421 catggagcta aggtcgtttc tataactgcg aaagacaagt tgcggaaaca acttcaaaaa
      481 gatatgaacc tttctagtgg ttctgtgagc atgtcgtccc aatacgccca cgattgctct
      541 atggaggaaa acggtataga aaaatttctt gaatttgttg gtctgccaca acctgaaatg
      601 tactctgccg acctgtcatt gtttgtccta caagcaggaa taaagtttct tgaagagcga
      661 aggccagata tcctctatct ctcactaact gatttcatac aacataccca cgctcctgga
      721 actgatgtcg caaatcaatt ttataaagca atggacgctc tctttggcca aatggtggaa
      781 ttaggtgctg tggttgcgtt gattgcagat cacggtatgg gtgacaaggc tgacgcaaaa
      841 ggagatccta aagtaatctg gctgcaggat atcttgaatg ccgaacttgg tgagggaata
      901 tgcacagtca tttgcccaat tactgatgcc ttcgtcggtc atcatggcgc tctaggtggt
      961 tttgtcaggg tatatatcaa tggcagagca gagcgcgaaa aagtcgttga aatagtccaa
     1021 gaccaaactg gtatcgagaa ggcctggtta gcagaggatg tcgccgaaga actggaacag
     1081 cctctagatc gagaaggcga cgttgcagta atgggtgaca agtggactgt gattggtggt
     1141 cgagcagtcg atcatgatct ctctgccttg aaaggacaca gacttcgtac ccacggtagt
     1201 ttgtacgagg cgaacattcc tttcgttatc agtgaaccat tgacaggctc ttatgccgag
     1261 cgtcacctta caaccacatt acggagccat gagatatttg actatgtctt gaatggtgtg
     1321 tcttaaacag gagaagatta cttttcctgg tgacaagttc tggaattact caattcccta
     1381 cgatagtctt gagtccagtg cggcgaacct ttgtaaagct gtacatacca tttgaacagc
     1441 tattagttgt tgctgaaaaa atcatcgatt aattcggttg taacaatata actgtgattt
     1501 ttcaatgata atgaaaatat tatgaattca attacttgtg cttttattgg ccttggtaaa
     1561 atggggcgtg aactttgtgg acgaattggt ggtgcagggt ataggactct tgcatatgat
     1621 cttgacccag aggcctcgaa gtacgcagaa gaaagttata aaaatgtaac ctcctccgct
     1681 ttattacctg ctgtgaaaaa ctctgcagta gttttcagtt gccttcctaa ctcacatcat
     1741 gtaagcgaag tagtcaataa tttgcttcaa caggaaggaa ctttaaataa agttcaatac
     1801 tgggtagata caacttcagg gcaccctggg gagtctcaaa aaatagcttc agaactcatg
     1861 tcacaggacg ttatattttt tgactgtgct gtttcaggag ggcctgcagg agcaactgct
     1921 ggtactctta ctgctatggt cggtggagac cacagtgctt tcggtgaagt tcaaagtata
     1981 atatcttcct ttgcaaaaaa tattgttcat ctaggtccaa gcggagcagg tcatgcagtt
     2041 aaagcagtca acaatacatt acttgcagca aatatctgta gtgtttcgga aggtttgatt
     2101 gcattgaaaa aatatggcat tccacttgat gttgcactta aagcaattag tacttcctcg
     2161 ggtcggtctt gggtcactca gcaaaggatg cccgagcatg ttttgacaag aggatttgac
     2221 tatggatttt cccttggttt gttatgtaaa gatgtggata cctgtatggg tatgttgcag
     2281 gaaagtaata tacccgcacc atcgctcagg gttacacgag aaataattca ggttgcaaaa
     2341 aatatcttgg gagatgagtc tgatcatttg gagatagtaa agataattga agattggtct
     2401 ggagaaacaa ttgaatgagt tttcatcact gtacaagtga ttatttcagt tggagaacaa
     2461 cacagtggtg tgaaagccac ttcaaagtca actgagcact gctgtaaacg tcaaacagtg
     2521 taaggctgct tatgctcaaa aaagaagaaa gtatgaatag atgaaaactc cagaattagg
     2581 tttttctcca aaaggacttt atataggtgg aaaatgggag gaaagtgtaa aagggaaaac
     2641 atttgaaagt atcaatcctg caaatatgga ttatttgggt gaattacctt tagctgatga
     2701 agaggatgta aatcgggctg ttattgcagc aaaggaggca ttctatgatt ggagtagaat
     2761 gccaatcaag gaacgtgctg gttttctaga tgctctagct gaaatattac aagacaaacg
     2821 tgatgaactg ggtttgatgg attgtcttga ttcaggtaac actatttctg gaatgaaagg
     2881 agatgttgat tggtctgcag atactttacg ttattttgcc ggtttgattg ctgaaatcaa
     2941 aggtgaaaca ttttcagaaa aacctggtca tttgaatttt acccgtcgtg aaccttatgg
     3001 agttgtggcc aagatcaatc catttaatca tcctctccgt ttttgtgcag agaaatctgc
     3061 agcagctctt gctgcaggaa atactgttgt aatcaaggcc tctgaacaag cacccttatc
     3121 aagtttacgg tttgctgaaa tctgtcacga agtccttccg ccaggtgtgg tgaatgtact
     3181 gacaggcgat gctgcatgtg gtcaggcaat ggtgcgcca
//
LOCUS       DTVD01000022            3168 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_12021_length_3168_cov_167.516865, whole genome
            shotgun sequence.
ACCESSION   DTVD01000022 DTVD01000000
VERSION     DTVD01000022.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12021_length_3168_cov_167.516865"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            277..1857
                     /locus_tag="EYO68_00660"
                     /pseudo
     CDS             277..1857
                     /locus_tag="EYO68_00660"
                     /inference="COORDINATES: protein motif:HMM:PRK13581.3-4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            2001..>3168
                     /gene="serB"
                     /locus_tag="EYO68_00665"
     CDS             2001..>3168
                     /gene="serB"
                     /locus_tag="EYO68_00665"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIC06348.1"
                     /translation="MNTSQNFLISIQGVTTENDVLERFTGQLEHLSFSIQDLHYLNFQ
                     SSLSILMRLELEQSTDKESLNKVLDKVAKQFKLELLIYPLPERSIAASSHPYILTLLS
                     QKLQPQLLTKLFCHLREKQLCVTEINPLDAGDLHVFEIKINADKPIERQQLMKELMGL
                     KSEYQLDLALQDDDLFRRNKRLILLDADMTFIQCEMINDMSRLAGKEAEVAEITQQTM
                     DGKIQFNEALQQRVALLKGVRLSDLERLILNIPYTPGVDRLVYILKTLGYKIGIVSGG
                     FTRVIDHIKQRFDLDYGFANTLEVKNGELTGCILGDILDGHQKGVILREIALKEKILP
                     EQVIAVGDGANDLEMLASASLGIAFNAKRYLRERAAGSLSLPNLDALLYFLGISK"
BASE COUNT          962 a          625 c          704 g          877 t
ORIGIN      
        1 ttatggttaa tttgttttca ccggtttttt aacaaataat cttgatcgac caaccgaaaa
       61 ttggtcaagg caatgaattt tatttgatag ctctcaagcc aactgagggt ccccgctcaa
      121 ccaacccccg atcctatctg tacacaggaa tcctgtccgt taacaattag cacccctgat
      181 ccagatgcag gggtttgcgg agggaggccc cacaaaatgt ggaatgatag tagacggcaa
      241 ttgtttacaa acagaacctg agtaaaaatt atttgaatgt acaaaattct gattaccggt
      301 tcaatacacc aaattggttt ggcaatcctg cgtaaagaaa aagatattga aatccagtat
      361 gctccggatc ttgcttttgc tgaaatattt aaaataatag caccgtttca ctgtattctc
      421 agccgctcgg aaacaccggt aaccagggaa ttgattgacg aggcgccaaa cctgaaagtt
      481 attgcccgtg ctgcagtcgg aataggaaat attgatgtaa attatgccac agaaaaaggg
      541 attcttgtca tcaatacacc aggcaaaaac acaaattctg ctgcagaact tacgatagga
      601 ctgcttctct ctgcaattag aaaaattatt cctgctcaca gccacatgag taagctcaag
      661 tgggaccgac atatttttac cggtatggaa ctgctcggaa aaacgattgg aataatcggt
      721 ctaggaaatg tcggacatct tgtagcacgt tacgcaaaag cctttgaaat ggaagtgctg
      781 gcttatgatc cttatattgc cgaagaagtg tttgagcgtt atcatgctga aaaatgcacc
      841 ctggaggaac tgatttcctc tgctgatata ataacgctgc atgtaccaaa aacggaggaa
      901 actacaggaa tgattggtgc tgaagagttc agtcgaatga aatccggagt ggtgattttg
      961 aacacagctc ggggcggaat tatccaagaa aagccattgt tggaagaatt aaaatcaggc
     1021 agggttgccg cagccggaat tgatacatgg gaagtggagc ctccaaaaca taatcctttt
     1081 cgggatctgc ctcaggtggt gatgtctcct catgttggcg cctctaccac agaggctcaa
     1141 aaaagaattg cggaatcaat tgcgacccag actccacggg ccctccgcgg agaagtagta
     1201 gactatcctg tgaatatgcc gagtgttcag gttttaagca gtggtcctgt cgcatcttat
     1261 gtctcacttg cagaaaaact tggtgtattt tcttcacaat atgttgactt tattcctact
     1321 catttagaaa ttaagtaccg tggtaaatta gccgaacatg atggaacact gcttagactc
     1381 tgttttttga agggcttgct gcaaagcagg aaaaattttg tgagttatgt caatgcagat
     1441 cagcaggcgg aaaatgttgg cctccatatc gaagaaacag atgacccagg atttacagat
     1501 tacgagagtg cactcaaatg cacacttgct ggagcaggaa agaagtttgc cattggtgga
     1561 gtagttttca gcggccccca tcctcgaatc actttgatca atagttttgt ctgtgaattt
     1621 gaagcggagg gtacaatttt ggtgtccact aaccaggatc gtccaggaat ggttggaatt
     1681 ttgggaacct gtttgggaaa aaatggtgtc aatatagacc agtttcaact gtcaagaaat
     1741 acacgtggcg gcgaggcttt ggcactgata cgtgttgatg atgacttgcc gaaatcagtc
     1801 gttgaagaaa ttcgcaaaaa ggaaggaatc acatccgttt ctaaaatagt tctgtaacag
     1861 caaaaggaag ttttatcagt caagccatct gctgctgtgc agagttttgg ttggaaagtt
     1921 caggccaaac cgagcattca agactttgaa caccttgttc aaacactttg tgaaattaat
     1981 ttaaaaaaca ctgactcaag atgaacacgt ctcaaaattt tctgatcagt atccaagggg
     2041 ttactactga gaatgatgtt ttggagcgtt ttacgggaca gttggagcac ctttcctttt
     2101 ccatacagga cctgcattat ctgaactttc agtcatctct ttctatattg atgcgccttg
     2161 aacttgagca atctactgac aaggaatctt tgaataaagt tttggacaaa gtggcaaagc
     2221 aatttaaact tgaactgctg atctacccac ttccggaaag gtctatagct gcttcaagtc
     2281 atccttatat tttaaccttg ttgagccaga aacttcaacc gcaattattg acaaaactgt
     2341 tctgtcacct cagagaaaag caactttgcg taacagaaat caatccattg gacgcaggtg
     2401 atttgcatgt ttttgaaata aaaattaacg cagataaacc cattgagcga cagcagttaa
     2461 tgaaagagtt aatgggattg aagtctgaat atcaacttga tcttgctttg caggatgacg
     2521 atttgtttcg gcgcaacaaa cgtttgattc ttctggatgc agatatgact tttattcaat
     2581 gcgaaatgat taacgatatg agcagattag ctggtaaaga ggcggaagtt gccgaaataa
     2641 ctcaacaaac catggacggg aaaattcaat ttaatgaagc cttgcagcaa cgagtggcct
     2701 tactcaaagg tgttcgactc agcgatttgg aacgtttaat tttgaatatc ccttacactc
     2761 ccggtgttga tcgtctggta tacattctta aaacactcgg ctataaaatc ggaattgtaa
     2821 gtggaggatt taccagagtc atcgatcaca tcaaacagcg tttcgatctc gattatggat
     2881 ttgccaacac actcgaagta aaaaatggag aacttaccgg ttgcatatta ggagacattc
     2941 tcgacggaca ccaaaaaggt gttatcctca gggaaattgc tctcaaagaa aaaattcttc
     3001 cggagcaagt gattgcggtc ggagatggtg ccaatgattt ggaaatgctt gcaagtgcca
     3061 gtttgggcat cgcatttaac gccaaacgtt atttgcggga acgtgccgca gggagcctct
     3121 ctcttcccaa ccttgatgca ttgctttatt ttttaggaat ttcaaaaa
//
LOCUS       DTVD01000023           12191 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1204_length_12191_cov_192.544743, whole genome
            shotgun sequence.
ACCESSION   DTVD01000023 DTVD01000000
VERSION     DTVD01000023.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_12191_cov_192.544743"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="EYO68_00670"
     CDS             <1..894
                     /locus_tag="EYO68_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HIC06349.1"
                     /translation="VISSASKEVVIGFNRPFVMIGERINPTGRKLLAKEMAEGDFSRV
                     EQDTLAQVAAGAHMLDVNAGIPLADEPKILADTIKLVQSLTDLPLSIDSSIVAALEAG
                     LEVYQGKALLNSVTGEEERLEVVLPLVKKYGCAVVAISNDETGISEDPDVRFEVAKKI
                     VEHAADYGIPSSDIVVDPLVMPIGAVNSAVRQVMSLVRRLQDELKVNTTCGASNVSFG
                     LPNRHGVNAAFLTMAIAAGLTSAITNPLHDSVMQAVMGADVMMGNDPHCQKWIKKYRE
                     PVAEGENGGKRERRRTRVKRG"
     gene            920..2290
                     /locus_tag="EYO68_00675"
     CDS             920..2290
                     /locus_tag="EYO68_00675"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIC06350.1"
                     /translation="MNISVFDLFKIGIGPSSSHTVGPMYAAKQFLFNCMEQFPLTKIS
                     SVKIELFGSLALTGKGHGSDTAILLGLEGEEPASVEPEQIPNRLKRIRKNRTLCLMNE
                     HKIYFEEEKSFIFKYEDLLPHHSNGMRFTVFDDDSTKLREENFYSVGGGFILNTEEIQ
                     NDNKDFGAQIPFPFRTCKELFEHCTKTGMTCRELMWINEQTWRSESDIWAGLLEIWGV
                     MQECTRRGMSSTETHLPGGLNVRRRAPELYKELVDNPETSPAEMMDWVSLFAMAVNEE
                     NAAGGRIVTAPTNGGGGVIPAVMHYCHRFRSEFNEDKIITFLLTAAAIGILFKEGASI
                     SAAEVGCQGEIGVACSMAAGGLAAVMGCTTSQIANAAEIGMEHNLGLTCDPVGGLVQI
                     PCIERNTMGAIKAINAARLARRGDGNHIISLDKVINTMHKTGKDMMHIYKETSLGGLA
                     VNIPEC"
     gene            2313..3188
                     /locus_tag="EYO68_00680"
     CDS             2313..3188
                     /locus_tag="EYO68_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC06351.1"
                     /translation="MSDESKGMILGVLGVTAFGLTLPATRFIIPYFDPVFIGLGRAVI
                     ASLVAAVLLIITKQKRPNRHQFHQLIVVASGVVIGFPILTAWAMQTVPASHGGVVLGV
                     LPLATAVAAAVVSNEKPSVGFWICGLAGSAIVILYTLLQGFGSFQFGDFLLIGAIVSA
                     AMGYALGGKLSKELGGWQVICWALVISFPFIVVPAWLEAPQTNIDSLPLSVLLSFLYL
                     ALISQLFGFFLWNKGLALGGISRVSQTQLLQPFVTLVASAYLINETVDLQTIVFAFLV
                     IGIVAIGKKMPVYER"
     gene            3304..4974
                     /locus_tag="EYO68_00685"
     CDS             3304..4974
                     /locus_tag="EYO68_00685"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIC06352.1"
                     /translation="MPSDIEISQNAVMKPIAEISQLLGVKSNDLEQYGKYKAKIEDTL
                     WESIKNNPDGKLILVTAITPTPAGEGKTCTSIGLAQAFGQLGVKHALALREPSMGPTF
                     GIKGGAAGGGYSQVLPMEDINMHFTGDLHAIAAAHNLLSAVLDNSMHFDNPLEIDPEK
                     VTWRRTIDLCDRQLRNAEVGLGSKFDGFPHQTGFDIVAASEVMTIMALAQDTEDLRER
                     LGRIVVAYNTSEKPVYARELNCIGSLCVILKDALKPNLVQTCEHTPVFVHCGPFGNIA
                     HGSNSVRATRLALKLAPYVVTEAGFAADLGAEKFVNIKCRQAGLKPNAAVLVATIRAL
                     KYHGGVEKDDLKIENLEALKTGCENLKTHAENVQKYGLPVVVAINRFPTDTPAELEIV
                     SKYCEEIGVESSLSEVVARGGEGGLDLANKIKRIVDETPGTPNFYYKNTDPIKRKIET
                     VAREIYRADGVDFTEEAEASILRLEENGLADWPICMAKTQASLSDDPKKRNAPRGWRL
                     QVREVKVSNGAGFIVALTGKMMLMPGMPRESAVQRIDIDSEGRITGLS"
     gene            5029..5409
                     /gene="folB"
                     /locus_tag="EYO68_00690"
     CDS             5029..5409
                     /gene="folB"
                     /locus_tag="EYO68_00690"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIC06353.1"
                     /translation="MGKKLSYTIRLNNVVVYGFHGVHPEEKTLGQRFEIDLEYRLKNP
                     ADPWKDEEHSTISYVNAHSIISRVCSEKSFNLIETLGNRIIEEMRESYLVDFIVVRIR
                     KPSVPIQGILDYVEVEVVWQAESE"
     gene            5430..5921
                     /locus_tag="EYO68_00695"
     CDS             5430..5921
                     /locus_tag="EYO68_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIC06354.1"
                     /translation="MTDIYEFLDANNVSYERHDHPAVFTVEESKILSPELNGASTKNL
                     FLRDKKGIRHFLVTVPEDKKVDLKALSSALDSSRLSFASPERLKTHLGIDPGSVSLLA
                     LANDSENNVEAFIDQEIWEDEAILCHPLVNTSTLVVPRKDMKLFLEKTGHGVRLIEVP
                     VGG"
     gene            6018..7331
                     /locus_tag="EYO68_00700"
     CDS             6018..7331
                     /locus_tag="EYO68_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC06355.1"
                     /translation="MNKKADAIIIGAGVIGSSVAFELAKRGYKTLNIDKLPTSGYGST
                     SNSCAIVRAHYSTWDGVAMAYEGFFYWDDWANYLGVEDERGMIKYMKTGSIMFKLQGE
                     DHSKKCLKFFEEIGVEHEIWDLEKLKERMPIYSHDQYDGTTRPDDDEHFFDKTGKKIE
                     GAVFTPGSGYVSDPQLSSHNLQVAAEANGGEFLFNNEIKEIRQEDGKVLGVTLKTGEQ
                     IDAPIVVNVAGPHSFVINRMAGVEEEMNIKTKALRHEVHHVPSPEGFDFEKDGFHTSD
                     GDNYIYFRPETGNTILIGSEDPLCDPKEWIEDPDNYNNQVTESQWRAQVYRCARRIPD
                     LKIPSRTSGVVDLYDVSDDWIPIYDKSALRGFYMAIGSSGNQFKNAPVAGHCMAELID
                     ACEKGHDHDANPLKVKTVYTGLELNMGFYSRNREINPNSSFSVNG"
     gene            7375..8514
                     /locus_tag="EYO68_00705"
     CDS             7375..8514
                     /locus_tag="EYO68_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIC06356.1"
                     /translation="MCGWRHDFHLHPELGFKEHRTAENVADLLEEFGIQIHRGIGGTG
                     IVGILQSGDGTRSIGLRADMDALPITELGTCEYRSQHDGVMHACGHDGHTSMLLGAAK
                     YLADSGNFNGKIVFIFQPNEEHGLGAPAMIKDGLFEQFEVDDVYGIHNIPGMPIGSFA
                     MRSGAITASESLFEINIKAQGGHAALPHMGVDAIIVATEIVQSLQTIVSRKLNPSLNG
                     VVSVTEFITDGSRNILPGSAILRGDARALSHQVNETIEARMRQIVEGVSLAHGVSAEV
                     QYETVFPSVINSPAPVLAAQQAASEVVGSAAVDADCEPKLFSEDFAHLAAAKPGCFVL
                     MGNGTQGENARPLHSSDYDFNDEALTVGSSFWVQLVEQQLSKSVV"
     gene            8514..8723
                     /locus_tag="EYO68_00710"
     CDS             8514..8723
                     /locus_tag="EYO68_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06357.1"
                     /translation="MFAERMERLRVRLAEADVDVALITDDDSVYYFTGFYDYLYMEFG
                     RPTILAVSRDGGSLLITPSTAVGVW"
     gene            8704..8883
                     /locus_tag="EYO68_00715"
                     /pseudo
     CDS             8704..8883
                     /locus_tag="EYO68_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010443227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
     gene            8986..9269
                     /locus_tag="EYO68_00720"
                     /pseudo
     CDS             8986..9269
                     /locus_tag="EYO68_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9285..9575
                     /locus_tag="EYO68_00725"
     CDS             9285..9575
                     /locus_tag="EYO68_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HIC06358.1"
                     /translation="MSFQLNCPNCGKRAVSEFTFKSEFKNRPAAEAEFSEWTDYVYFR
                     ENNMGRQIEWWYHRSGCQSWFLAERDTLNNSDHKSFWYGDLGQHSKNSGGEA"
     gene            9575..>12191
                     /locus_tag="EYO68_00730"
     CDS             9575..>12191
                     /locus_tag="EYO68_00730"
                     /inference="COORDINATES: protein
                     motif:HMM:PF04324.13,HMM:PF13510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC06359.1"
                     /translation="MAKRITKHQNCELPNLGEEISFTFNGKRIKAFAGDSITSALIAS
                     GSTLLSRSFKYHRPRGAYDVFGQGHESLVTVNHEPNMLADRIQVQNGMVVKSQNVWPS
                     VEFDLGEVNDLFVPMLPNGFYYKMFHKPKWLWPIAEHQIRKVAGLGKIDVAGKHVHRR
                     YEKHYRFPDVCVVGGGPSGLAAAKGALDEGKQVLLIDDNPQLGGHSLHSIFPVNNCEN
                     ESLNGIPENQAVQKLIKELAANPNLEVMVNTSVFGLYEDNLVAAQSGPDLFKIRAESV
                     VLAPGATDRHLVFENNDRPGILTARGVEKLIMCHAVLPGEDTVVVTTHDGGFHTALLL
                     KGAGANVVAVVDGRESGSAGEFEEQVRNLAVPVYKGLTVHAAHGKKRIRRIDIGPIAG
                     GDSHKSFDCDLLVMAVGFKPQVNLLSMGSKRPEWDAERQILRVTDLPSGMYSTGEVHG
                     SAGFTRLFSEGIKTGKAAAKKKSVESSKRSEDELIMALPADIESGGKNHFICKCMDVT
                     RYEAQASIDEGYDQVESLKRYSSMGMGPCQGKACHEAVARLAAQDTGLSSLDAVPTTM
                     RPPFSGVSFGNLAGRAPHLSPISRTPFHQCHIDLGVKFLNAGQWKRPDSYIDPQKEVG
                     YVRNGLGMIDVSTLGKIEISGPDAIDFLQFMLPGKYGKFEVGRTRYSIMVGEDGILFE
                     DGTISHLEQGKYYLTTTTGNQDAINSLFQWWLLVKDFDVQVKNLSLANAAVNVTGAES
                     REFLQKLVKIDMSNEAFPYMHCRQASIAGVPVSFYRIGFTGELGYEIHFPAEYGESMW
                     NHLMAEGEEFALKPFGVETQRILRLEKGHLIPGTDSDALSSPYETGVGFAIKDDKADF
                     VGKAFLQHFKKRGIEN"
BASE COUNT         3541 a         2394 c         3004 g         3252 t
ORIGIN      
        1 gtaattagtt ctgccagcaa agaagttgta attggcttca accgaccgtt cgtaatgata
       61 ggcgaacgta tcaatcctac cggacgtaaa cttttagcca aagaaatggc tgaaggtgat
      121 ttcagccgcg tggaacaaga cacgctggca caggttgccg ctggagcaca catgctggat
      181 gtgaatgcag gaattccatt agccgacgaa ccaaaaatat tggctgacac aatcaaactg
      241 gtgcagtcac tgaccgatct tcccctttca atcgactctt caattgttgc ggcactggaa
      301 gccggacttg aagtttatca aggtaaagca ttattaaatt cggtaactgg ggaagaggaa
      361 cggcttgagg tagttttgcc gttggttaaa aaatacggtt gtgcggtggt ggccatttcc
      421 aacgatgaaa ccggaatctc cgaggatccg gatgtgcgtt ttgaagtggc aaaaaaaatt
      481 gtggaacatg ctgcagatta cggaattcca agttccgata tagtagttga tccgttggtg
      541 atgcctattg gagccgtcaa ttcagcggta cgacaagtga tgagtttggt aaggcggtta
      601 caggatgaac tcaaagtcaa cactacttgc ggtgcctcaa acgtcagttt tggcttgccc
      661 aatcgccacg gagtgaatgc agcatttttg acaatggcaa ttgctgccgg actgacttca
      721 gctataacta atccactcca cgattcggtg atgcaagccg taatgggtgc agatgtgatg
      781 atggggaatg atccgcattg ccagaaatgg attaagaaat accgggaacc tgttgcagaa
      841 ggagaaaacg gagggaaacg ggagagaaga cgtacaagag tcaagcgagg ataaatgtgt
      901 ataaaaaaca agaggcaaaa tgaatattag tgtatttgat ctgttcaaaa ttggaattgg
      961 gccttcaagt tcacatactg taggtccaat gtacgcagca aaacaatttt tgtttaattg
     1021 tatggagcag tttccactga ctaaaatttc ctcggtcaaa attgagttgt ttggttctct
     1081 tgcactgaca ggcaagggac atggctctga cacagcaatt ctactgggac tagagggtga
     1141 agaaccagct tctgttgaac cagaacaaat accaaaccgc ttaaaacgta tcaggaaaaa
     1201 cagaactttg tgcctgatga atgaacacaa aatttatttt gaagaagaga agtcttttat
     1261 ctttaaatat gaagatttat tgccgcacca ctcaaatgga atgagattca ctgtttttga
     1321 tgatgacagc acaaaattac gagaagaaaa tttttattca gtaggtggtg gatttatctt
     1381 gaatacagaa gaaattcaaa acgataataa agattttggt gctcaaattc catttccttt
     1441 tcggacatgc aaagagcttt ttgagcactg cacaaaaact ggtatgacat gcagggaatt
     1501 gatgtggatc aatgaacaaa catggcgttc agaatctgat atttgggcgg gactgcttga
     1561 aatttgggga gtaatgcagg aatgcacacg gcgcggaatg tcctcaactg agacacacct
     1621 tccgggtgga ctgaatgtac gcagacgcgc tccggaatta tataaggaat tagtggacaa
     1681 ccctgaaact tcgcctgcag aaatgatgga ttgggtgagt ttatttgcca tggctgtcaa
     1741 tgaagagaat gctgccggag gaagaatagt aaccgccccg acgaatggcg gaggtggagt
     1801 tattcctgcg gttatgcact attgccatcg tttccgatcg gaatttaacg aagacaaaat
     1861 cattacattt ttgctcacgg ccgcagcaat aggcatcctt ttcaaagaag gtgcttctat
     1921 atctgcagca gaagtcggat gccagggaga aattggagtt gcctgttcca tggcggcagg
     1981 aggactcgcc gcggtaatgg gatgtactac cagccaaatc gcaaacgcag cggaaatcgg
     2041 aatggaacat aatttaggcc tgacctgtga tcccgttggc ggccttgttc aaattccgtg
     2101 tattgaacgc aatacgatgg gagctataaa agctataaat gctgcaaggt tggcaagacg
     2161 tggtgacggg aaccatatta tttcacttga taaagtgatc aacacaatgc acaaaaccgg
     2221 caaagatatg atgcatattt acaaggaaac ttcattaggt ggacttgcag tcaatatacc
     2281 tgaatgttga actggaaggt aaatttcaaa gaatgagtga tgaatcgaaa ggtatgatct
     2341 taggagtttt gggtgtgact gcatttggtt tgacactgcc tgccactcgt tttattatcc
     2401 cttactttga tcctgttttc ataggtttag gacgagctgt aattgcttca ttagtagccg
     2461 cagtgctact gataataaca aagcagaaaa gaccaaatcg ccaccagttc caccagctta
     2521 tagttgtagc atcgggtgtg gttatcggtt ttcccatttt aaccgcatgg gcaatgcaaa
     2581 cagttccagc atctcacgga ggagtagtcc tgggtgtgct gcctttggcg acagcagtcg
     2641 ctgctgcagt ggtttccaat gaaaaaccat ctgtaggttt ttggatttgc ggacttgccg
     2701 gaagtgcaat agtcatttta tatacacttc tgcagggctt cgggagtttc cagtttggtg
     2761 attttttact aattggagca atcgtgagtg ctgcaatggg ttatgcttta ggaggaaagc
     2821 tctcaaaaga acttggtggc tggcaggtga tttgttgggc cctggtgatt tcgtttcctt
     2881 tcattgtggt tccagcgtgg cttgaagctc cacaaacaaa tattgacagt ctgcctttga
     2941 gtgtgctttt aagtttttta tatttagcct tgatcagcca gttgtttggt tttttcctct
     3001 ggaacaaagg gctggcttta ggaggaatat cacgtgtcag tcaaacacaa ttattacaac
     3061 cttttgtaac tttagttgct tctgcctact tgatcaacga gacggttgat ttacaaacta
     3121 tcgtctttgc gtttttggtt atcgggatcg tcgcaattgg taaaaaaatg cctgtttatg
     3181 aacgttgaga agaaattatt gatttggaat ttaacttata aaatatttta actttttatt
     3241 tttatcatta gtatgaaaac agacctaaat aaattggaag aactctatcc tgaaatgccc
     3301 agcattccgt ctgatattga aatatcacaa aacgcggtca tgaaaccaat agctgaaatt
     3361 tctcagctac tcggggtaaa atctaatgat cttgagcaat acggcaaata taaagctaaa
     3421 atcgaagaca cgctctggga aagcatcaaa aacaatccgg acggcaagct tattcttgtt
     3481 actgcaatca cacctacacc ggcaggtgaa ggaaaaactt gtacttccat tggtctggca
     3541 caagccttcg gacaacttgg tgtaaaacac gccctcgctt tacgtgaacc aagtatgggg
     3601 ccgacatttg gcatcaaagg aggtgctgca ggtggcggtt attcccaggt tctgcctatg
     3661 gaagatatta acatgcactt taccggtgat cttcatgcaa ttgcagctgc acataattta
     3721 ttgtctgccg ttctggacaa ctcaatgcac ttcgacaacc cgctagagat agatcctgaa
     3781 aaagtgactt ggagacgcac aattgattta tgtgacagac aactccgtaa tgctgaagta
     3841 ggacttggtt caaaattcga tgggtttccc catcaaaccg gtttcgatat tgtagctgca
     3901 tcggaagtaa tgacaatcat ggccctggca caggacacag aagatttacg cgagcggctt
     3961 ggacgaattg ttgtggccta caacacttcg gaaaaaccag tttatgctcg tgaattaaac
     4021 tgtataggtt ctttgtgtgt aatcctgaaa gatgccttaa aaccgaacct tgtgcagacc
     4081 tgtgagcaca ctccggtttt tgtacactgt ggaccttttg gtaacattgc ccatggttca
     4141 aacagtgtca gggctacacg cttggctctt aaacttgcac catacgtggt gacagaagca
     4201 ggttttgcag ctgatttagg tgcagaaaag tttgttaaca tcaagtgccg tcaggccgga
     4261 ttaaaaccca atgccgcagt tttggtcgcc actatcagag ccttgaagta tcacggtggt
     4321 gttgaaaaag atgatttaaa aattgaaaat cttgaggcct taaaaacagg ttgtgaaaat
     4381 ttaaaaacac atgcagaaaa tgttcagaaa tatggactgc ctgtggtagt tgccatcaac
     4441 cgttttccaa cagatactcc tgcagagttg gaaatcgtaa gcaagtattg tgaagaaatt
     4501 ggtgttgaaa gttcactcag cgaagttgtc gcgagaggtg gcgaaggtgg acttgatttg
     4561 gcaaataaaa ttaagcgtat agttgacgaa acaccaggca caccaaattt ttattataaa
     4621 aacacggatc caattaagcg taaaattgaa actgttgccc gtgaaattta ccgggctgac
     4681 ggtgttgatt tcactgaaga agctgaagct tcaattctac gcctggaaga aaatggttta
     4741 gcagactggc cgatttgtat ggcaaaaacg caagcctcgc tttcagatga tcctaaaaaa
     4801 cgcaatgcac cacgtggatg gcgtttacag gttcgtgaag tgaaggtctc aaacggagca
     4861 ggtttcattg tggctctaac aggaaagatg atgctgatgc cgggaatgcc gagagaatcg
     4921 gctgtgcaac gtatcgacat agattcggaa ggacgtatca ccggactgtc ctgatttaat
     4981 aaacagcaaa aatagagttt taaatactac ttatttcagc cgcattatat ggggaaaaaa
     5041 ctaagttata ccatccgatt aaacaatgtt gttgtttacg gatttcatgg agtccatcct
     5101 gaggaaaaaa ccctgggaca gcgttttgaa atagatttgg aatacaggct gaaaaatccg
     5161 gcagatccct ggaaagatga agaacactct actatctctt atgtgaacgc acacagtatt
     5221 ataagtcgag tctgctcaga gaaaagtttt aatttgatag agacccttgg caaccggatt
     5281 attgaagaaa tgagagagag ctatttggtg gacttcatcg ttgtacgtat ccgaaaacct
     5341 tctgtaccta ttcagggtat attggattac gtggaagtag aagtggtctg gcaggcagaa
     5401 tcagaatagg tattaaatga acaattgtaa tgacagatat ttacgaattt cttgatgcta
     5461 acaatgtttc ttacgaacgt catgaccatc cggcagtttt tacagtggag gagtccaaaa
     5521 tactttctcc ggaattgaac ggagcatcca caaaaaattt atttttgcgg gacaaaaaag
     5581 gcatccggca ttttttggta actgttccgg aagataaaaa agttgacctc aaagccttgt
     5641 cttccgcatt ggattcatcc cgtttgagtt ttgcgtctcc ggaacgcttg aaaacgcatt
     5701 tgggcattga tccgggttcg gttagtttgt tggctttggc gaacgatagc gaaaataatg
     5761 tggaggcttt cattgaccag gaaatttggg aagatgaagc aattttgtgt catccgttgg
     5821 tcaataccag caccttggtg gttcctcgca aagacatgaa attattcctc gaaaaaaccg
     5881 gacatggagt gcggttgatt gaagtaccgg ttggtggcta aacaccggaa tctaatattt
     5941 tgcttgctga atattttgta aaacttgttg tgcaggaatt aatttaataa aacaaccatt
     6001 cattaagaag ggaataaatg aacaaaaaag cagatgccat tatcattgga gccggagtga
     6061 tcggttcctc ggttgcgttt gaattggcga aacgcggtta caaaacactc aacatcgaca
     6121 aattgccgac atccggatac ggttcaacga gtaattcgtg tgccattgtg cgagcgcatt
     6181 attcaacttg ggacggagtt gcgatggcct acgagggatt tttctattgg gacgattggg
     6241 cgaattattt gggggtcgaa gacgagcgcg gaatgatcaa atatatgaaa accggttcca
     6301 tcatgttcaa acttcaagga gaagatcaca gcaaaaaatg cttgaaattt tttgaggaaa
     6361 ttggcgttga acacgaaatc tgggacctgg aaaaattgaa ggaacggatg cctatttatt
     6421 cgcatgacca atatgacggc accacccgtc cggatgatga tgaacacttt tttgacaaaa
     6481 ccgggaaaaa aatcgaaggt gcggttttca ctccgggttc cggatatgtc agtgatccgc
     6541 aactgagttc gcacaatttg caggtggctg cagaagccaa cggcggagaa tttttgttca
     6601 acaacgaaat taaggaaatt cgccaggagg atggaaaagt gcttggggtt acactcaaaa
     6661 ccggagaaca aattgatgca cctattgtag tcaatgtagc cgggccgcat tcttttgtga
     6721 taaaccgcat ggcaggtgtt gaggaagaaa tgaacattaa aaccaaagcg ttacgtcacg
     6781 aagtacatca tgtgccttca cctgaaggct ttgattttga gaaagacggc tttcatactt
     6841 ctgatggaga caactacatt tatttccgcc cggaaaccgg aaacactatt ctcatcggca
     6901 gtgaagaccc actatgcgat cctaaagaat ggattgaaga tccggataat tacaacaatc
     6961 aagttacgga atcccaatgg agggcccagg tttaccgttg tgcacgcaga attccggact
     7021 tgaaaattcc ttcacggaca agtggagtgg ttgatttgta tgatgtttct gacgattgga
     7081 ttccgattta tgacaaatcc gccctgagag gtttttacat ggccatcggc tccagcggca
     7141 accagttcaa aaatgctccg gttgccggac actgtatggc agagttgatt gatgcttgcg
     7201 aaaaaggaca cgatcatgat gcaaatcctc tgaaagtcaa aacggtttat acgggtttgg
     7261 aactcaatat gggattttat tcccgcaacc gtgaaatcaa cccgaacagt agtttttcgg
     7321 tgaatggttg atgcaagagg ttgagcagtg aaaaccaaac acttacagga agcaatgtgc
     7381 ggatggagac atgattttca tctccatccg gaactgggtt tcaaggaaca ccggactgcg
     7441 gaaaatgttg ctgatttact ggaagaattt gggattcaga ttcatcgggg aatcggcgga
     7501 acaggcattg taggcatttt gcaatcgggc gacggaacgc ggagtatcgg actccgggca
     7561 gacatggacg ctttgccgat tactgagttg ggcacttgtg agtatcgttc tcagcatgac
     7621 ggagtgatgc acgcctgcgg acacgacgga cacacctcca tgttgttggg agccgcaaaa
     7681 tatcttgccg attcaggaaa ttttaacgga aaaatcgttt tcattttcca acccaatgaa
     7741 gaacacgggt tgggtgctcc ggccatgatt aaagacggac tgtttgagca gtttgaggtg
     7801 gatgatgttt acggaattca caatattccg ggaatgccga tcgggtcttt tgcaatgcgt
     7861 tccggagcaa ttacggcaag tgaaagtttg tttgaaatca atatcaaggc gcagggagga
     7921 cacgccgctt tgccgcacat gggtgttgac gcgattatcg ttgccacaga aatcgtgcaa
     7981 tcattacaaa ctattgtctc ccgtaaactg aatcccagtt tgaacggtgt ggtttccgtg
     8041 acagaattta tcaccgacgg aagccgtaac attctacccg gttcagcgat tttgcgtggg
     8101 gatgcccgcg cactcagcca tcaggtcaac gaaacgattg aagcacgcat gaggcaaatc
     8161 gtggaagggg taagtctggc ccacggtgtt agtgcggaag ttcagtatga aacggtattt
     8221 ccatctgtaa tcaattcacc tgctcctgtt cttgccgctc agcaagccgc atcggaagta
     8281 gtcggttcgg cggcggttga tgccgattgc gaaccaaaac tattctctga agattttgca
     8341 cacctggcgg cggccaagcc gggatgtttt gtgttgatgg gcaacggaac tcagggggag
     8401 aatgcacggc ctttacattc ttctgattat gattttaatg acgaagcatt aaccgttggt
     8461 tcatcatttt gggtgcaatt ggttgaacaa caattatcca agagcgtagt gtaatgtttg
     8521 cggaaagaat ggaacgcctg cgtgtccgat tagcggaagc cgatgtagat gtggcgttga
     8581 ttaccgatga cgacagtgtt tattatttca ccggttttta cgactatctg tacatggagt
     8641 ttggccgccc gacgatttta gcggtttccc gggatggcgg aagcttgttg attacccctt
     8701 ccaccgcagt tggtgtttgg tgacaaaacg attctgcaac ccggaatggt gcttgccgta
     8761 gatggctctg tcagtgtgaa aaaaactttc cgggcgcagg tgggagacag ttttatcgtc
     8821 acagaaaacg gctatgagcc gctgaccgaa tttgccaaag atttagagga tgtagtgtgc
     8881 taaccagctg actatccagc tacttgttta ttgcaacagt tgtaaataca ggagaaatat
     8941 tgtttatatc gtggaatcaa tccgaacagt agtttttcag tcaaaatgga agataaaaag
     9001 ggaattttat ataaaggtgg ttgtctttgt ggtaagataa tttttgaaat tatccgtgga
     9061 attcaagaaa atatttttgc aaacaaggcg gttctccgat tatgagcaaa aaatctacta
     9121 caccggacac aattcgagtc cgacttggta catttgaatc agacataatg gaacatccaa
     9181 ccgcacatat tttcgttgga tcaaaggcaa acggggataa cataacagat gacttgcctc
     9241 aaaatgggtc aaacaaacca aacagctaat tttaaaaacg tactatgagc tttcaactaa
     9301 attgccctaa ctgcggcaaa cgtgccgtga gtgaatttac tttcaaaagc gaattcaaaa
     9361 accgtcctgc tgcagaagca gaattttctg aatggacgga ttatgtttat ttccgagaaa
     9421 acaacatggg acgtcaaata gaatggtggt accaccgcag tggctgccaa agctggtttt
     9481 tagcggaacg agataccttg aataactctg atcacaaaag tttctggtac ggagatttgg
     9541 gacaacactc taaaaacagc ggcggagaag cctgatggca aaacgtatta caaaacatca
     9601 aaattgcgaa ctcccaaatt tgggagaaga gatctctttc acattcaacg gcaaacgaat
     9661 caaagccttt gccggtgaca gcattacctc agcactgatt gcttcaggta gcactttgct
     9721 ttcacgaagt tttaagtatc accgcccccg cggagcatat gacgttttcg gtcagggaca
     9781 tgaatctttg gtcacggtca atcatgaacc gaacatgctg gcagatagaa ttcaggtaca
     9841 gaacggaatg gttgtaaaat cgcaaaatgt ctggccttca gtggagtttg atcttggaga
     9901 ggtcaacgat ctgtttgtgc cgatgctgcc gaatggtttt tattacaaaa tgtttcacaa
     9961 accaaaatgg ctctggccga tcgcagaaca tcaaatccgt aaagttgccg gactgggaaa
    10021 aatcgatgtt gcaggaaaac atgtacatcg gcgctacgaa aagcattacc gttttccgga
    10081 tgtgtgcgtg gttggcggtg gaccttccgg actggccgct gcaaaagggg cactggatga
    10141 aggaaaacaa gttttgttga ttgacgataa tccgcaactc ggcggacatt ctttgcacag
    10201 tatttttccg gttaataatt gcgaaaacga atcactgaac ggaattccgg aaaatcaggc
    10261 agtacaaaaa ttaattaaag aacttgctgc gaatcctaat ctggaagtga tggtcaacac
    10321 cagcgttttc gggctttatg aagacaacct ggtagctgcg cagagtggtc cggatttgtt
    10381 taaaattcgc gctgaatctg tcgtattggc accgggagca accgaccgtc atttggtttt
    10441 tgaaaacaat gatcgaccag gaatcctgac tgcccgcgga gttgaaaaac tcatcatgtg
    10501 ccacgctgtg cttcccggag aggatacggt tgtggtgaca actcacgacg gtggttttca
    10561 cactgcgtta ctgctcaaag gcgcaggagc gaatgttgta gccgtagtag atggacgtga
    10621 atccggaagt gctggagagt ttgaggagca agtccggaat ttggctgttc cggtttacaa
    10681 aggcttaacc gttcatgctg ctcacgggaa aaaacgtatc aggcgcatcg atatcggccc
    10741 aattgccgga ggagattcac ataaatcttt tgattgtgat ttgctggtca tggctgtggg
    10801 ttttaaaccg caggtgaatt tactctcgat gggaagcaag cgtccggaat gggacgcaga
    10861 acgccaaatt ttgcgggtga ctgatttgcc ttcaggaatg tattccacag gagaagttca
    10921 tggatcagca ggatttacac gtttattcag cgaaggaatc aaaaccggaa aagctgctgc
    10981 aaagaaaaaa tcagtagaga gttccaaacg ctctgaagat gaacttatca tggccttgcc
    11041 tgccgacatt gaatcgggcg gcaaaaatca tttcatatgt aagtgcatgg atgttacacg
    11101 ttatgaggcc caggcttcaa ttgacgaggg ctatgatcag gtggaatctc tcaaacgata
    11161 ctccagcatg ggaatgggcc cctgtcaggg caaagcatgt cacgaagctg tagcacgtct
    11221 tgcagcgcag gatacaggac tttcctcact agatgctgta cccactacga tgcgtcctcc
    11281 tttttccggt gtatcatttg gtaacctggc aggtcgcgcc ccgcatttgt ccccgattag
    11341 ccgcacaccg ttccatcagt gtcacattga cttaggcgtg aaatttttga atgcaggaca
    11401 gtggaaacgg ccggattcat acatagatcc tcagaaagag gtcggctatg tccgtaacgg
    11461 tttaggtatg attgatgtca gtacactcgg aaaaatagaa atcagcggac ctgatgcaat
    11521 tgattttctg caatttatgc ttcctgggaa atatggaaaa tttgaagtag gccggacacg
    11581 ttactcaata atggttggtg aagacggaat tctctttgag gacggtacaa tttcccatct
    11641 tgaacaaggg aaatattatc ttactactac taccggaaat caggatgcaa taaattccct
    11701 ttttcagtgg tggctattag tgaaggattt tgatgtgcag gtaaaaaatc tcagtttagc
    11761 aaatgcagca gtcaatgtca ccggagctga atcaagggaa tttttgcaaa aactggtcaa
    11821 gatcgacatg tctaatgaag cctttccata catgcactgc cggcaggcaa gtatcgcagg
    11881 agttccggta tcattttacc gcattggttt caccggagaa ctgggttatg agattcattt
    11941 tccagctgaa tatggggaat caatgtggaa tcatttaatg gccgaaggtg aagaattcgc
    12001 gctcaaacct tttggtgtgg aaacacaacg tattttacgt ttggaaaaag gtcacttgat
    12061 tccgggaact gacagtgatg ccttaagcag tccgtatgaa accggagtcg gcttcgcgat
    12121 taaggatgac aaagcggatt ttgtcggcaa ggcctttttg cagcatttca agaagcgcgg
    12181 catagaaaac a
//
LOCUS       DTVD01000024            3158 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_12086_length_3158_cov_195.195295, whole genome
            shotgun sequence.
ACCESSION   DTVD01000024 DTVD01000000
VERSION     DTVD01000024.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_3158_cov_195.195295"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="EYO68_00735"
     CDS             complement(<1..359)
                     /locus_tag="EYO68_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIC06360.1"
                     /translation="MNVEQFSLREYAGYETSLSEHPDEELTQVGPETPCGEYLRRFWH
                     PVYLTNDLCDLPVALKILGEELVLFRDLSGTLGLVHKRCPHRQASLEYGKCEEHGIRC
                     CYHGWHFDTDGKLLDAPG"
     gene            complement(378..1799)
                     /locus_tag="EYO68_00740"
     CDS             complement(378..1799)
                     /locus_tag="EYO68_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIC06361.1"
                     /translation="MSMSYHENFADALPEMARVKEEKKRLEAAGVKYILSCWIDLLGV
                     PKTKPVPISDFELLCMGKGPQFAVHSISFVPELGPADSDQIPVPDLDSLIICPWDSTC
                     AWMFADLWWEGHPYNLCPRSALKRIIKDTADQGYAVMAGIEPEFMVMKWQDGQPVKAF
                     DNDPNSGQGLRPRRQAFGYDVEYSIDSMSFLGELIDIIEGLGWNLHDVVAEGAYSQFE
                     LDFHYTDVLRMADRFVFLRVLLKEVAKRHGMFVTFMPKTTIGDWRSGAHLNTSMQPID
                     KEGTNFFESKSGGWADSVFNAIGGLLRHGGALTAIACSTVNSYNGLVPRVGGFEGGTV
                     TWAPTHMTYGYNNRSAMLRLPQSRFCIENRAADMCMNPYLSLGLTTAAMTEGIVNNYS
                     PGEPLNEDLYSMSKEDLERSGAQQLPRNLLEAIEILRGDEFARKVLGVAMIDSFLHYK
                     TDEWERYHQAVTDWEVEEYLRLY"
     assembly_gap    1919..2018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2049..>3158
                     /locus_tag="EYO68_00745"
     CDS             2049..>3158
                     /locus_tag="EYO68_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIC06362.1"
                     /translation="MTDLKRTQLFSAFDENENVKHGLPSAAYTSKDFYELESKLVFAK
                     SWTFVGFSHDLAQVGDITPLEVAGQPLILVRSSKNKIRAFHNVCRHRNLKLVDKPRNC
                     KSLITCPYHNWCYNLDGQLRAAPFFGGQKTELPKDFKLKEHGLLEVPCELFHDWIFVN
                     LNCDTVSFESHLAPLRKQLAGIDLDDFVVVVSIEFGEIKTNWKLLMENFIEPYHVQFV
                     HKNTTNQPLVDHYVVSDGHCLGSAVDLTSEQQEQAQEGTLSVSSRYLTLFPNFVLGVY
                     YPDQIGVHLNQPVSAGITCQRRVIYQHKDSDQSTEAVEKISRLWESVHKEDHAMCERL
                     QEGRHSDAAQQGGFLSPYWESSVRSFQELVADAIRP"
BASE COUNT          844 a          745 c          673 g          796 t
ORIGIN      
        1 cccggtgcgt caagcaactt accgtcggtg tcgaagtgcc agccgtgata acaacaacgg
       61 attccatgtt cctcacattt tccgtattcc agagaagcct gccgatgcgg acaacgcttg
      121 tgaaccagac ccaacgttcc ggagaggtct cgaaacaaaa ccagttcttc gccaagaatt
      181 ttaagggcaa caggtagatc acagagatca ttggtgagat aaaccggatg ccagaaccgt
      241 cgtagatatt ctccacaagg agtttccggc ccgacttgag tcagttcctc atcggggtgc
      301 tctgacaaac tggtttcgta tccagcgtat tctcttaaac tgaattgctc tacattcatc
      361 acaatacccc atcgaaatca gtacaatctc aaatattcct cgacttccca atcggtgacg
      421 gcttggtggt agcgttccca ttcgtcagtt ttgtagtgaa ggaacgaatc aatcattgca
      481 acaccaagta ctttccgcgc aaattcatca cctcgcaaaa tttcaatcgc ttccagcaaa
      541 tttctgggaa gttgttgggc accggagcgt tcaaggtctt ctttactcat gctatacaaa
      601 tcctcgttca acggttctcc tggagaatag ttgttgacga tgccttcggt cattgcggcg
      661 gtggtcaagc ccagcgagag gtagggattc atgcacatgt ccgccgcccg gttttcaata
      721 caaaatcgac tctgcggcaa gcgtaacatg gctgatcgat tgttgtagcc gtaagtcatg
      781 tgagtcggtg cccaggttac agtgccccct tcaaaaccac cgactcttgg aacgagtccg
      841 ttgtaagaat tgaccgttga acaggcaatc gcagtcagtg ctccgccgtg tcgcaataac
      901 ccgccgattg cattaaatac cgaatcagcc cagccgccgg atttgctttc aaaaaaattc
      961 gtcccctctt tatcaatcgg ctgcatggag gtattcaaat gggctccgga acgccaatcg
     1021 ccgatggtgg tttttggcat aaatgtcaca aacatgccgt gacgtttggc cacttccttg
     1081 agcaaaacac gcaaaaaaac aaagcggtcg gccatgcgca aaacatccgt gtaatgaaaa
     1141 tccagctcaa actgagagta tgcaccctcc gccacaacat cgtgtaaatt ccatcccagc
     1201 ccttcaataa tgtcaatcaa ctctccgagg aaactcattg aatcaattga atattccacg
     1261 tcgtaaccga aagcctgtcg gcgcggacgc aaaccctgac cggaattcgg atcattatca
     1321 aaggctttga caggctgtcc atcctgccat ttcatcacca tgaactccgg ttcaattccc
     1381 gccataaccg cataaccctg atctgctgta tccttaatga tgcgtttgag tgcactgcgc
     1441 ggacaaaggt tgtaaggatg tccctcccac cacaaatcgg caaacatcca ggcacaggtt
     1501 gaatcccacg ggcagatgat caggctgtcc aaatcaggaa ccggaatctg atcggaatcc
     1561 gccgggccga gttccggaac aaaggaaatg gaatgaaccg caaactgagg tcccttgccc
     1621 atacacaaca attcaaaatc gctgatcggt acaggtttcg ttttcggtac acccaacaga
     1681 tcaatccagc acgacagaat gtatttgact ccggccgctt caagtcgttt cttttcttct
     1741 ttgacacgcg ccatttccgg cagtgcgtcc gcaaaatttt cgtgatagct catgctcatg
     1801 ctgttctcct tcttgagttg ataaaaaaaa tgttgttaaa gtgcctggcg ttttaaaaga
     1861 aactgatttt tgaacatttc aatgccggcc attatttcaa attgatcaat gtcctttgnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa actttccata aaaaaagaga
     2041 attgctttat gaccgattta aagagaacac aattgttttc agcattcgat gaaaacgaaa
     2101 atgttaaaca cggtctaccc tccgcagctt ataccagtaa agatttttat gagctggaat
     2161 caaagcttgt ttttgctaaa tcctggacat tcgtaggttt ctcgcatgac cttgctcagg
     2221 tcggtgacat aactcctttg gaggtggcag gacaaccctt gattctagtg cgtagttcaa
     2281 aaaataaaat ccgtgcattc cacaatgtct gccgccatcg aaacctgaaa ctggtggaca
     2341 aacctcgcaa ttgcaaaagc ctgattacct gtccttatca caattggtgc tataatttag
     2401 acggtcaatt acgagccgct cctttttttg gtggtcaaaa aactgaattg ccgaaagact
     2461 ttaaactgaa agagcacggt ctgctggaag ttccgtgtga actttttcat gattggatat
     2521 ttgttaatct caactgtgac acggtatcat tcgagtcaca ccttgctccc ttgagaaaac
     2581 aattagcagg aattgatctg gatgattttg ttgtggtcgt cagcattgaa tttggtgaaa
     2641 tcaaaacaaa ctggaaactc ttgatggaaa atttcattga accgtatcac gttcaatttg
     2701 tacataaaaa caccacaaat cagccgttag tggatcacta tgttgtaagt gacggacatt
     2761 gtctgggaag cgcagttgac ctgacttcgg agcaacagga gcaagcgcag gaaggaacac
     2821 tgtccgtcag ttcgaggtat ttgactttgt ttccgaactt tgtgctggga gtttactatc
     2881 ctgatcaaat tggagtccat ctcaatcagc ctgtgtccgc tgggataacc tgccaaagac
     2941 gggtgattta tcaacacaaa gattctgatc aaagcactga agcggttgaa aaaatcagca
     3001 gactgtggga aagtgtacac aaagaagatc atgcgatgtg tgaacggctt caggaaggac
     3061 gccactcgga tgcggcccaa caaggcgggt ttttatctcc gtattgggaa tccagtgtcc
     3121 ggagtttcca ggaattggtt gccgatgcca ttcgtccg
//
LOCUS       DTVD01000025           36196 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_121_length_36196_cov_387.797183, whole genome
            shotgun sequence.
ACCESSION   DTVD01000025 DTVD01000000
VERSION     DTVD01000025.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 36196)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36196)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36196
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_36196_cov_387.797183"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="EYO68_00750"
     CDS             <1..870
                     /locus_tag="EYO68_00750"
                     /inference="COORDINATES: protein motif:HMM:PF13413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIC06363.1"
                     /translation="EEEESLQLLGQQIREARITKNLSLESVSGHLHIPLNTLQSIEDG
                     CKGNVLPPVFLRGLVRSYCLFLGLENTGIIDKTDRLLKSDDENEQLNLKSLRPVYENR
                     ESKPIGIILTVLVLIVGGFFVYSFYFLQTPFSFNGDNETITEPLVVEVEPIIEPVQKS
                     TPAIQESVSPAEPSPEIPEVSTEITLVEKKQTENTQIIQPVAVQNTVEPLVLEVEASE
                     DTWISIAVDKKETKDVLLEADKIQQWEAKKQYLLTLGNTHAVRVLLNGREIETNRAHQ
                     LLTDWVIDASLLP"
     gene            894..1838
                     /locus_tag="EYO68_00755"
     CDS             894..1838
                     /locus_tag="EYO68_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HIC06364.1"
                     /translation="MQQFRVFEHGKRYRPLSQHYLERFGEKVFKVSISVAESCPNREG
                     HNGAAVCIFCDEWGSAAYHQQREKPVREQIQINREAIRKRYRAEKFLIYFQAYTNTFG
                     RFRELEYFYKEALKEKDVVGIVVGTRPDCLPKRVLQKFSEIARENYLSVELGVQTFDD
                     AQLQFLSRGHDSASSLKAIRKLKTLPDINLCVHLMFGLPDETDAQIRKTAEILSEQKV
                     DGVKLHNLHVLRNTPLEKLYRESHFKPVDLEEYSRKVALFLENLSPKIAVHRLAAVAS
                     RWDELVAPQWTREKMKTTQFIDDFLAAKETWQGRCFEV"
     gene            1971..2489
                     /gene="rsfS"
                     /locus_tag="EYO68_00760"
     CDS             1971..2489
                     /gene="rsfS"
                     /locus_tag="EYO68_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIC06365.1"
                     /translation="MTDKCGIRTFYHLILSYKRNLVAEKDTVLKNSDNKVAYELNYEN
                     NLDKAIADDSDRALIKRILELLEDAKAEEIVLIDAQHRSSLADYLLICEGRSQLHCRG
                     IAENVEHNLKREGEASLGIEGEREGNWVLLDYGNIILHVFHPEIRKYYRLEELYEQGP
                     GQNSTKMSMTSE"
     gene            2504..3463
                     /gene="murB"
                     /locus_tag="EYO68_00765"
     CDS             2504..3463
                     /gene="murB"
                     /locus_tag="EYO68_00765"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:PRK13905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIC06366.1"
                     /translation="MSNKEKPEWLEELEKAPLTSRLRRKESLKRFNTWRIGGVAECLI
                     DVVNAEDLSLLLPFISKHRIPWFILGKGSNLFIPDTAWPGIILHLSGEFKSWVPLEEN
                     NSSACKNRVTAGAALADVTFAQRCVAQGWGGMEFLIGIPGTIGGAVAMNAGAHGGETA
                     DFLQEAEWMDMEGNLHTSARQNLEFRYRYSDLMGNYGRIITSAVFEFQESDPETVEKK
                     LLECQQFRMEKQPYNQPSCGSVFKNPPGDFAARLIEASGLKGKIRGGAQISPIHANFI
                     VNLGNASSTDILSLIDTVRETVFSKHSINLEPEVQILQSSVYG"
     gene            3561..4655
                     /locus_tag="EYO68_00770"
     CDS             3561..4655
                     /locus_tag="EYO68_00770"
                     /inference="COORDINATES: protein motif:HMM:PF08478.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HIC06367.1"
                     /translation="MQDLKRQSNRRPVVRKLRTSSMRVRKNHSNPIPGLSVKLRKPNT
                     EPGYSNSWLPPYFVENEPAFLRMKMRWLIQKFIGFCGLIFILWLVGGSVWVGQWFFQQ
                     PLSRVNIKGNLLLKDADVLFASGLRQGQRLIDLNPYQVAARLQTHPIVQKTDIRRKFP
                     DEIHLIISEHQPVALLQLSQQTNVFSSASLSKTKYVLIGNNQRLLKLLTFDELRDSPY
                     KNLPLINGLTVPSVTLGSSLDSPVLERGLRFLETFQTMAAAQKAELQNTQIKLEGQFR
                     IVDWSSQPIHIDISDPLNLKINWPLNSSELQPGSPEPLRTVPLTIQMGSRDFGERLRT
                     FQNIYPVLGKQHPGLKSIDLRYKNRVMLVP"
     gene            4717..6264
                     /locus_tag="EYO68_00775"
     CDS             4717..6264
                     /locus_tag="EYO68_00775"
                     /inference="COORDINATES: protein motif:HMM:PF05157.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06368.1"
                     /translation="MTMALLDELVQEEDLDPEVMGQTSSAFKNLPELEKIRRLIQMDL
                     LEEPHLLRNMSDRYNLPLLSTTLDEVQNYENLPLSKQLYERTGILPMRLGSKCACLLS
                     VQSNWLKMNQVAFHLGEPIYWYLAQQQQIEALLETSHLQIEDADISATDRIHHLFEEA
                     VSRRGSDIHLEPEKNQLRVRFRIDGYLQDSAPMPMSFHPLIFSRIKLLAGMDIAVRRR
                     PQDGHYTYSSNSNRIFDVRVSTLPGQFGGKLVLRLLDQTPVQHKLEALGFFKNDLEML
                     YQASQAPSGLILMVGPTGSGKTTTLYAILNALNSQEKNILTIENPVEYHIEGITQVSV
                     KPEQGLGFADTLRAALRQDPDVLLIGEIRDEETANIAVKAALTGHLVLSTLHSSDAVT
                     AIQRLLNLGIAPDLLAETLTVIVSQRLVRRLCSHQNGNKSNPHTSDKNCLRCRGTGYS
                     GRIPVYEILKVNSLVRDRIQAGDTGRKLIRPEPELYFHTMEQTAQRLTKEGLTDWKEV
                     QPLLLNV"
     gene            7160..8125
                     /locus_tag="EYO68_00780"
     CDS             7160..8125
                     /locus_tag="EYO68_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIC06369.1"
                     /translation="MCQTLEAQCTPKLKDQTNPQQTVQWLDQIYQDFLLFNQSLQEFA
                     SNRRSFEEQANTIQQLRGQLVETEQDLSEHMEFLKVNQATVIRLTKKISKTTGIRLSK
                     TGKTKRVKAKTGRGGPTILDSLSLDSPQTLLSSGSLRSFLYNNSDNYYETVAQLEDLS
                     QLIERNQSFWRHTPTVIPVRSRALSDRYGMRKDPFTKKREYHAGIDFRAKRGTPVHAP
                     ADGTVRIADRKSGFGLLVELQHGRGFFPGKKNSVRYRTRFAHLSKIKVRRGQKIKRGD
                     LLGLVGSTGRSTGPHLHYEIIINGRRTNPLFPISRFNPDQRLYRN"
     gene            8147..8503
                     /locus_tag="EYO68_00785"
     CDS             8147..8503
                     /locus_tag="EYO68_00785"
                     /inference="COORDINATES: protein motif:HMM:PF04519.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIC06370.1"
                     /translation="MPNQNTAANKGLGFLGEDLYVEGTIHSQKKMVVSGTVDGSVFCD
                     QEIVVSETGQVKGKIEGNIVLVAGKINGDLLVHERLEVNVTANIKGEVQVPSGKLLIQ
                     EGAKLDAQCTILYDKT"
     gene            8583..9053
                     /locus_tag="EYO68_00790"
     CDS             8583..9053
                     /locus_tag="EYO68_00790"
                     /inference="COORDINATES: protein motif:HMM:PF04977.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HIC06371.1"
                     /translation="MQYPTSKNSLKQQNKPVEQSKLLKNHDRILYVSGLFSPPKVQET
                     PVVGMAKETEEWRSLFSLVLIAGFGVVMLIISVIGSYGILATSRLEAHEQMLLQKLES
                     MKVEKTLMQEEVFALKTSQEYIELIARKELGLVHSTELIYYLPPSAKDSSRTDF"
     gene            complement(9059..9493)
                     /locus_tag="EYO68_00795"
     CDS             complement(9059..9493)
                     /locus_tag="EYO68_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008079344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIC06372.1"
                     /translation="MKELLKTFPVVTKIPVIWGDMDSFQHVNNVIYFRYFESARIQYF
                     ETLGWLKIIEKMGIGPILGSTSCRYRIPLTYPDTVFAGAKITELHEKRFTMEYLLVSE
                     KHADPVAEGTGIVVCYNYQKNQTTPIPEVIQQSIEKLEDRIF"
     gene            9505..10182
                     /locus_tag="EYO68_00800"
     CDS             9505..10182
                     /locus_tag="EYO68_00800"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIC06373.1"
                     /translation="MSEINKQKEGLPQKIALIICNGNPPPEKLLHQLWQEADYRVAAD
                     GGANQLYCYNLIPDAVVGDFDSIKPEVRKKLPNSKLFHVKEQDTNDADKAVRHCLKRE
                     CTEIHILGAEGSRNDQFLSSLEILFKYTPNVRLILWTPLERMEFILDNWKENLPPGTI
                     LSLLPLFGGAQGVVTHGLEFSLDGHDLLPGKTPSGVSNLVISNPVSVTLKKGQLLLVV
                     QHTTNYS"
     gene            complement(10192..11106)
                     /locus_tag="EYO68_00805"
     CDS             complement(10192..11106)
                     /locus_tag="EYO68_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIC06374.1"
                     /translation="MENIGGIFLVFLNVVTPVFILVVIGYFVGPRLKIDARSLSRTAY
                     FVFIPAFVFNIISEAKIDSELALQMLSFILVAQIAVALLGFLVGKALRQSREITAAFV
                     LIATFGNVGNFGLPLIVFRLGETARTFATVYFVATVFISFVICVGVASWARSGGVTAV
                     FSVFKTPALIALIPALVFNITDVEVPIFLSRLSGLLGQAMIPVMLITLGVQMGEIPKI
                     KINFNVFAASTVRLIGGPVLALLIVPYFGLEGLERSTGILQAAMPAAVLASIIALEYK
                     LLPEFVTTTVLFSTLYSILTLTVILTFI"
     gene            complement(11173..11808)
                     /gene="nth"
                     /locus_tag="EYO68_00810"
     CDS             complement(11173..11808)
                     /gene="nth"
                     /locus_tag="EYO68_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIC06375.1"
                     /translation="MKSAKIASLFRKLSDSIPNPKTELCYRNPFELLISVILSAQATD
                     VSVNKVTPELFSAFPTPEEMFEAGSEKVFAYIRSIGLAPTKSKNIAATCGQLVKLHKG
                     EVPDKRKDLEALPGVGRKTANVILNTAFGVPVIAVDTHIFRLSNRTGLAPGETVIKVE
                     QKLMSVVPAKWKKDAHHLLILHGRYVCRARKPDCGHCVISQECELPDKIVT"
     gene            12073..12591
                     /gene="moaB"
                     /locus_tag="EYO68_00815"
     CDS             12073..12591
                     /gene="moaB"
                     /locus_tag="EYO68_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIC06376.1"
                     /translation="MKQLDFLPLRISILVVSDSRTEETDTSGKILTERLTEEGHILAE
                     KCIVPDDIYQIRAVLSRWIVDPAVDIILSTGGTGLTGRDGTPEAVFPLFDKVIEGFGE
                     LFRRISYDEISTSTIQSRVTAGVANGTYIFCLPGSSGACRTGWDKILATQLDIRSRPC
                     NFAELIPRLTEK"
     gene            complement(12682..13785)
                     /locus_tag="EYO68_00820"
     CDS             complement(12682..13785)
                     /locus_tag="EYO68_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HIC06377.1"
                     /translation="MKEEAIILTSGLFDSHNAKTAFGLIRNSEQYEILAVIDQNFAGQ
                     DAGKLVDGQVLDIPIHATINEFLRARSSRNKLALEESGRGVYAIIGIATQGGVIPEGL
                     RIELLEALSSGMHVVNGLHHYLSDDPELVQRAEKNQAKLLDIRKPKSAKDHAFWTGNI
                     LNVRAPRIAMFGMDCALGKRTSSLWLRDACRKQGIRTEMIYTGQTGKLQGGRYGFILD
                     STLNDFVSGELEEAIFRCDQEENPDLMLLEGQSSLRNPSGPCGSEYLCSALAKGVILQ
                     YAPKQKYYLTDDDRKLWPMPPIEEELELIRLYGSQTLAITLNSYELTKKELESEQQKL
                     EERLGLPVVCPMEEGMERLVPVVQEFIASEAEN"
     gene            complement(13995..14486)
                     /locus_tag="EYO68_00825"
     CDS             complement(13995..14486)
                     /locus_tag="EYO68_00825"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC06378.1"
                     /translation="MIPSSAHHIQGISFCLEPSPKEMMKMDSLCFSAAWSEQDYREMQ
                     EQPTFSNWLLQVPTAAQVGMLSFQSVPPELQILRLAVLPDWRKQGLAQFMLEQLEIVA
                     KSANLESLWLEVNSANKAATALYYKQGYQETGIRKNYFINPLGDALLLKKILKYDDKQ
                     AAQ"
     gene            14699..>15652
                     /gene="rpsB"
                     /locus_tag="EYO68_00830"
     CDS             14699..>15652
                     /gene="rpsB"
                     /locus_tag="EYO68_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018023790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIC06379.1"
                     /translation="MAVISMKQFLAAGVHFGHQTKRWHPNMAPYIYGIKNGVHVIDLR
                     QTLTKLKEAYEYVKASAEEGRVPLFVGTKIQIQRIVKEEADRCGCPYINFRWLGGLLT
                     NFGTVKQSIARMKTFEEVAGEDGSYEGVIKKEALMMERKRVKLERSLGGVRNMKSVPS
                     MLFITDCHKEQLALKEAQKLGIPVVGIADTNCDPTGIDFVIPGNDDSPRAVALYANVI
                     ATAVMEGLAARKQTRIESITEEKAAAKKAKPAAKAKEKETDVVSEAKAEEEKKAEAKA
                     KEKKAEETPAKIKEEKTEAAPGPKSAETKVAAEKAKPEVKAK"
     assembly_gap    15653..15752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15768..15956
                     /locus_tag="EYO68_00835"
     CDS             15768..15956
                     /locus_tag="EYO68_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06380.1"
                     /translation="MKAKSEAKAKEKKAEVVTEAKSAVEKTPAKKTAATAKKAETASG
                     EKVPAAKATKAKKLETKK"
     gene            16023..16901
                     /locus_tag="EYO68_00840"
     CDS             16023..16901
                     /locus_tag="EYO68_00840"
                     /inference="COORDINATES: protein motif:HMM:PRK09377.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HIC06381.1"
                     /translation="MSIGSINKMAAITAAAVKELREITGVAMMDCKKALVEADGDLEA
                     AQDYLRKKGQAKALKKSSRETSEGAVSFTVSTDGKRAGLVKVACETDFVARNDIFQDF
                     ISKLANQVSEQGGENVLQQTLVEGEGSVESLLTEKIAELGENMQILDCRKMEISNGVH
                     GGYIHSNGKIGVAVPLETDKPCDDERLKTLARDIAMHIAAFPAEAVNADQVSKEVLEK
                     EKEVFTAQARESGKPENIIEKMIEGRLKKFLKEICVESQPFVKDPQLSVSQLVENTAK
                     ELGVKINFNSFAKFQF"
     gene            16911..17633
                     /locus_tag="EYO68_00845"
     CDS             16911..17633
                     /locus_tag="EYO68_00845"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIC06382.1"
                     /translation="MSPKYKRILLKLSGETLGGKRGTGFDYDTIRALAESVISIHNLG
                     VEVGIVIGGGNIFRGSKSAEGNVGRVAGDHMGMLATVINSICLQEMLEQRGILTRVLS
                     AIELNAIAEPYIKRRADRHLEKKRVVIFAAGTGNPFFTTDTAAVLRASEVGAEIVIKA
                     TKIDGVYDKDPKLHSDAVRYDVLNYDEVLKKHLKVMDATAIAFCRDNQLPILVLDYQE
                     PDAILRAVCGETVGTLIKQRGS"
     gene            17630..18199
                     /locus_tag="EYO68_00850"
     CDS             17630..18199
                     /locus_tag="EYO68_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIC06383.1"
                     /translation="MTMENTQEVEEMVDHKMDITIESLKVDLSGIHAGRVSPAMLDAV
                     KVEYYGNPTPVNQVANISTPEPQMLAISPWEKTMIKEIERSLQAANLGFSISNDGNII
                     RAVTPPFTEERRKDYVKQIKKIGEDSKIAVRNVRREGNDNLKQMEKDKLISQDEEKVA
                     QDHVQKVTDKHTNMVDELVTAKEKELMTF"
     gene            18207..19463
                     /locus_tag="EYO68_00855"
     CDS             18207..19463
                     /locus_tag="EYO68_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIC06384.1"
                     /translation="MLAALLHSIWQYQIFKSVVFRGGMAFLTAYFLIVWVMPSVIRAF
                     RKRGITSDFITTPADIKPYTGAPPIMGGGVLIMGILLGALLWCNLNQYVLALLVIMLS
                     FGIIGAVDDIAKVINKRRVESGHETRKAFSEKADGISGRWRLTAQFAVSLLVVTGLYL
                     FVDIDGHLVVPLVPLKTAYPYLPKYLFIPFMTIIIVGGANAVNLTDGMDSLATVPLMT
                     CAMFVGLVAYIGGDPEFAARLKIPSLSHDIKELAVLSALVISAGVAFLKYNSPPALIY
                     MGDLGALALGSMVSAMFIFVKAELFLPIVGGVFVFSVLSSIIQRTFFRFIVWRKGRPT
                     AERYRFFLRSPYHHHLQRLWTYSEKKQEVVSVWIMLQNKLGIDPVPEENKLLNPQEVN
                     SRVIWHMHLKSIWLFVFTLIIYFKIR"
     gene            19471..20283
                     /gene="fabG"
                     /locus_tag="EYO68_00860"
     CDS             19471..20283
                     /gene="fabG"
                     /locus_tag="EYO68_00860"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIC06385.1"
                     /translation="MSERKSGTITSAQQKSLTVNKTTETAMNFEGQIALITGAGRGIG
                     KTIALKLAGSGADIVLADMSPDVAEVRGEVEALGRKCMTFEVDVTNIETIDAMVKNIT
                     DELSGIHILVNNAGITQDNLFLRMKSEQWSKVIDVNLNGVFNVTKAVIRLMVKQRTGK
                     IINISSVVGFTGNPGQVNYSSTKSALVGFTKSLAREVGVRGITVNAVAPGFIDTAMTQ
                     ALNESQQEVILQQIPLGRMGDAEDIANAVAFLASKEASYITGTILHVNGGMY"
     gene            20382..20618
                     /gene="acpP"
                     /locus_tag="EYO68_00865"
     CDS             20382..20618
                     /gene="acpP"
                     /locus_tag="EYO68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIC06386.1"
                     /translation="MEEIQAKVIQIISEQLGKDESEIKMSSHFIEDLDADSLDTVELV
                     MALEEEFEVDIPDEAAEKITTVQSAVEFIAESKA"
     gene            20707..21942
                     /gene="fabF"
                     /locus_tag="EYO68_00870"
     CDS             20707..21942
                     /gene="fabF"
                     /locus_tag="EYO68_00870"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HIC06387.1"
                     /translation="MGNRVVVTGLGCMSPLGNDVSSSWEACTTGQSGIDRITHFDPEL
                     FRCQIAGEIKNFQLPEIIPIKEAKKMDLFIQYSIGATQEALLDANLEITEDLEEEVGV
                     SMGVGIGGLGCIERYSKIIEEHGPKRVSPFFIPMTLSNLAPGYVSLLFRAKSYTACTV
                     SACASSNHAIGTAARTIERGDAKVVISGGAESSVTPLGIAGFASMHALSTRNDDPQKA
                     SRPFDKDRDGFVMGEGSGMLILEDYEFAKARGARIYCEITGYGFSSDAHHVTSPSSEG
                     PTRAMKMTLKDSGLAPEEIDYVNAHGTSTPVGDLNELRAIKMALGEKAAQQLSISSTK
                     SMTGHLLGAAAAIEAIFSIKALHHNFIPPTINIENLDPECDLDVTPNTGKSKEMRVAM
                     SNAFGFGGTNASIIFQKID"
     gene            22036..23841
                     /locus_tag="EYO68_00875"
     CDS             22036..23841
                     /locus_tag="EYO68_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC06388.1"
                     /translation="MSSTEYQVDRRELQFVVNETLKAGKLCELPDFAEFDEEMFTMII
                     NEASTFSEQVLGPLNENGDRQGCRIENGSVVTPDGFANAWKQLGEGGWLSMNMPPEYG
                     GQGLPEVISMIAKETQLAANQGFTIGASLTSGSANLIYTFGSEEQKITFCEKMFGGKW
                     SGSMCLTEPQAGSAVGDAATSATKQEAGHYLIKGTKQFITNGDHDMAENIIHLLLART
                     TDAPEGTKGISLFIVPKIRMDGTPNDVKVISIEHKMGINGSPTCLLSFGDNASCHGYL
                     LKAENIGMAQMFQLMNEARLLVGLQGLAGAGAAVQNAWAYAKERTQGPSAIHPGEKAA
                     IIEFPDVRRMLMQMKAVTEGLRALMYTTAWYTDMAHHGPDESREKYQDLLDLHIPVCK
                     AFGTDQGFDVARIGIQVLGGVGFTQDFPLEQNARDQKIASIYEGTNGIQALDLVSRKF
                     TAKKGQLLKVLEQELSWFDNHAPEDELAGWVAEWESYRTLMQESIAGLKKIGEEQGKD
                     GYVLYAVNMLDLMGDVLCCFYLLKQADSAQQKWKALLAEATSQAELLEENEEAQFYWN
                     KLRTTEFYVWSVLPRALSNAKTIKNANLAPLNACL"
     gene            23935..24489
                     /locus_tag="EYO68_00880"
     CDS             23935..24489
                     /locus_tag="EYO68_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIC06389.1"
                     /translation="MNLHFATSNPNKLSELSELLEYRLTPLELDLQEIQTTDLQELVK
                     HKLKQAYEHIHAPVIVEDTSLYFKAWNELPGPLVKWFIENLGLAGMVSALSQFKDKSA
                     QAVCCLGYTNNGKSMYLFEGKVKGVIVKPRGSLNFGWDAIFQPSEHEQTFGEMDPEDK
                     NRISPRGKAATKFKHFLTQSAKQT"
     gene            complement(24452..25408)
                     /gene="ispH"
                     /locus_tag="EYO68_00885"
                     /gene_synonym="lytB"
     CDS             complement(24452..25408)
                     /gene="ispH"
                     /locus_tag="EYO68_00885"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIC06390.1"
                     /translation="MNTNSAGAPLNLVLASPRGFCAGVDRAITIVEKALEMYGAPIYV
                     QHEIVHNKHVVQRLRNDGAVFVENIDEIPEGSHAIFSAHGVSPEVRKRAENRKLQVLD
                     ATCPLVTKVHREAQRYAQKEHTIILIGHHNHVEVKGTVGEAPEHIFVVGTVEEVSDLK
                     IPDEKKVGYITQTTLSLDDTAEIITALKERFPEIKGPAKDDICYATQNRQNAVKALSK
                     EVDLVLVVGAQNSSNSVRLLEVAETTGVKARRIESAAELDPEWLEEVRNVGITAGASA
                     PEDIVQGIVAEISKMSSSSSVRDLEIVQEDVTFALPTVLRNA"
     gene            25527..25868
                     /locus_tag="EYO68_00890"
     CDS             25527..25868
                     /locus_tag="EYO68_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06391.1"
                     /translation="MSEEDKRRKASWKRLKGTTNFYPTYVDGNLDKVTPAAATHSTQF
                     QTVIPRYDLSMSETRNAGTGPTFLETAYQPPTENPDPEKQSPDTPPESSERKLKRPRH
                     KKKKIRTDFWE"
     gene            26116..27456
                     /locus_tag="EYO68_00895"
     CDS             26116..27456
                     /locus_tag="EYO68_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIC06392.1"
                     /translation="MFETLSDKLSGSLRKVRGRGRLTEENVSKTLNEVKMALLEADVN
                     FRVVKAFLRSVKSRAVGEEVQGSLTPGQQFIKIVNEELTEMMGGANSGLTEPETAPLV
                     TMLVGLQGSGKTSSAGKLARLYKTEGKRPYLIPADIYRPAAIQQLQVLADTLGISCYP
                     SRTDQNPLDIVQNGLEAAKQEEADAVFIDTAGRLHIDQELMEELSRIKDSVMPHEILF
                     VADAMTGQEAVSVAKGFNDLLDITGVMLTKMDGDARGGAALSIRAITQKPIKFIAVGE
                     KLENLEAFHPERIASRILGMGDMLSLIEKVEDSFSEREALQIQKKLRRNEFTLEDFRN
                     QLRSMRKMGSIKDVIGMLPGMNASSVNDANIDDKKLIHIDAIISSMTIKERNNHKLMN
                     GSRRIRIAKGSGTTVSEINRLLKQFIQMRKMMQKLTKVSNPGKAMRMMQDMMPN"
     gene            27526..27768
                     /gene="rpsP"
                     /locus_tag="EYO68_00900"
     CDS             27526..27768
                     /gene="rpsP"
                     /locus_tag="EYO68_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIC06393.1"
                     /translation="MVKIRLARGGTKKKPYYRVVVADQRCKRDGRYLERIGFYNPMVS
                     ENRFGIDEERVKHWLSVGAQPTERVSKLMKQAKIKV"
     gene            27810..28364
                     /gene="rimM"
                     /locus_tag="EYO68_00905"
     CDS             27810..28364
                     /gene="rimM"
                     /locus_tag="EYO68_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HIC06394.1"
                     /translation="MSVFNDYDELTWVATIVGAHGIKGAVRVKYFTDTPDYYLNVELF
                     FLESEGQLQSLKVLQIRPTKSGWIVLFEEIDSRTAAEKLKSCRLLLPDDQLRPLESSE
                     VFVHQLIGCSVEDKNGRFLGEISDVLETGANNVYEVRNGKSSFMIPDVPHVVLELDVE
                     SRRMKIDPLPGLIEETAPESNVTL"
     gene            28351..29055
                     /gene="trmD"
                     /locus_tag="EYO68_00910"
     CDS             28351..29055
                     /gene="trmD"
                     /locus_tag="EYO68_00910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIC06395.1"
                     /translation="MLRFEVLTLFPDIFTSFLEESLIKRAIEKQYLNVSLVNFRKHGH
                     GKHLQVDDTPYGGGPGMVLRIEPIAQTLKEQKEFHRSSGRETHTILVTPQGRPFSQEK
                     ARELSRKKAVLTLICGRYEGFDERLRSLVDEEISGGDFICMGGEVITMTMIETISRLV
                     PDVLGNQESAVHESFTQPMLEFPQYTRPASFEGMEVPEELKSGNHKIIAQWREEQALE
                     RTKNRRPDLLQCKTKS"
     gene            29105..29473
                     /locus_tag="EYO68_00915"
     CDS             29105..29473
                     /locus_tag="EYO68_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIC06396.1"
                     /translation="MSTVMEKIEQSQLRSGLPELRVGSSVRVNYRITEGNKERIQAFE
                     GVIIGKHCSARNNKATFTVRKVTQGYGVERIFPLHSPRIDSIDVIKVGKVRQAKLYYL
                     RARRGKAARIKERITARPSA"
     gene            29421..30119
                     /locus_tag="EYO68_00920"
     CDS             29421..30119
                     /locus_tag="EYO68_00920"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIC06397.1"
                     /translation="MEKQPGSKNASRHGHQHNFSPTLELETAAQGRGFQQVAGVDEAG
                     RGPLAGPVMVAAVILGKDWNAEHPLNDSKKLSSTKREQLFDVICSEALAFKIVSISAE
                     EIDRLNILQATLHGMLRCLTEIEPAPDYALVDGNRFPQTTIRGEAVVKGDARSKSIAA
                     ASILAKVTRDRVMVENAKRYPEWGFEGHKGYPTKQHRAAIEKYGLSPLHRRSFRLKSL
                     SKLDRSSTAQLNLL"
     gene            30089..30475
                     /locus_tag="EYO68_00925"
     CDS             30089..30475
                     /locus_tag="EYO68_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIC06398.1"
                     /translation="MVYCSIEFAVTKARQQTGAAGEQIACNFLQEQGYRIIERNHRSR
                     LGELDIIAAYGEFLIFCEVKTRRGSSGPHPSLSVTAKKIGKLRQLGELYLSRKHLTYL
                     QPRFDVIAVQLADGKPPNIDHFINAF"
     gene            30494..30763
                     /gene="rpsT"
                     /locus_tag="EYO68_00930"
     CDS             30494..30763
                     /gene="rpsT"
                     /locus_tag="EYO68_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIC06399.1"
                     /translation="MAHHKSALKRVRQTIKRTAQKRSQRADLRTVIKKFRLILDGENM
                     DQAREAYSGVQKNIDKAVTKGILHKRTGARYKSRLALSLSKTVAS"
     gene            30914..31531
                     /locus_tag="EYO68_00935"
     CDS             30914..31531
                     /locus_tag="EYO68_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIC06400.1"
                     /translation="MDSHFLMRRIHSLTGVVPVGLFLVYHLYLQLYLHSGAETYNTAV
                     NSFYDSPLAIWTLVIVVYIPLFFHAFLGVRLIFESTVQPSYTYFSHLLYWLQRISGIG
                     VLLFILAHVWNTQFGPWISGTWGTHFEHLSSGFSDPESGIITKTVYLLGVLGAVFHFA
                     NGFNTFCMTWGIALTPKSQIRVRIFSILLFLILSASALYALSAIW"
     gene            31546..>33143
                     /gene="sdhA"
                     /locus_tag="EYO68_00940"
     CDS             31546..>33143
                     /gene="sdhA"
                     /locus_tag="EYO68_00940"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HIC06401.1"
                     /translation="MSKQRVIVIGGGLAGLAATMKMAELGMSVLLVSFLPVKRSHSVC
                     AQGGINGAVNIKGEGDSPDIHFYDTVKGGDFLAHQPLCKDMCHHAPYIINLMDRLGVT
                     FNRTPEGNLDFRRFGGTLYHRTAFAGATTGQQLLYALDEQVRHYEVQGLVEKMEWHEY
                     LGAVLNGDGRCVGAVIHNLRSGEISTEKGDAVILATGGPGLVYGRSTNSMVCTGTAVT
                     SAYLQGAKYGNGEFIQIHPSAIPGRDKLRLMSESARGEGGRVWVPRKAGDSRKPKEIP
                     EKERFYFLEERYPLFGNLVPRDVGAREIYDICVNDKLGVHGEMKVYLDLTHLTREFLD
                     HRLGGILEIYEKFTGVDPREEPMEIFPAVHYSMGGIWTDYTRTEEGLIDHQSPQNQMT
                     SITGLYAAGEADYQYHGGNRLGANSLLSCIYTGLMMSPGVLNYVKNVPEAADDVPEKL
                     FTEATKQWQEKYAEIKKMSGNENPYALHREMAEEMISNVLIVRENSKLQATLEKIDEF
                     EERWKNVNCVDTTDWSNPVPSFIN"
     assembly_gap    33144..33243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33353..34099
                     /gene="sdhB"
                     /locus_tag="EYO68_00945"
     CDS             33353..34099
                     /gene="sdhB"
                     /locus_tag="EYO68_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIC06402.1"
                     /translation="MPDTITFKIKRQDSESSGSYWETFVIPHVPNSNVISCLMDIQAN
                     PENSKGEKVAPVVWECNCLEEVCGSCTMVINRKVRQSCSALVDQLEKPITLEPMSKFP
                     VIRDLAVDRSRMFGALKKVQAWVTADGYHDLGPGIREPQNHQEIAYKLSECMTCGCCV
                     EACPQYSLNNNFLGAAAISQSRLFNMHPTAKNIADERLDTLMQEGGIADCGNAQNCVE
                     ACPKSIPLTESIAEIGRQVSGKLWSNIFQA"
     gene            34135..35193
                     /locus_tag="EYO68_00950"
     CDS             34135..35193
                     /locus_tag="EYO68_00950"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIC06403.1"
                     /translation="MSTNPSSDSRKRFLLIRTDRVGDTILTFPAVTALRNNYPEAFIS
                     FLAQPYTVPLIEQYAGIDLLLSYEPEGRHRGWKGVLKLSHQLRELNFDAAILFYPRPE
                     LAFALLKAKIPLRIGTGYRWYSFLLNRRIYEHRKDCRKHESEYNLSLLESLMPDKITQ
                     PHYEFKQWTPEPWWDDFQTELKSKDYVIVHSGCGASAPNLNEEQYKLIIRLLLEKTDW
                     TVLLTGVSGEENVVNALAADFPEERVKKTVGRFSLPELFTVIRNASLLIASSTGPLHL
                     ANAAGTPLLGFFCPAKPHTPTRWGPYDQQQWVVTPNLDWPEICELKNCPHGGCLNQLS
                     DDEITEILCTQRLKTIHK"
     gene            35190..>36196
                     /gene="pgi"
                     /locus_tag="EYO68_00955"
     CDS             35190..>36196
                     /gene="pgi"
                     /locus_tag="EYO68_00955"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510852.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIC06404.1"
                     /translation="MNHKQNKGMTKSVTWQNFQQHYLDLPELGMALDTSRMNVPDKLP
                     NELEMLFRNAFQQMQNLEKGGIANPDENRMVGHYWLRNPELAPNAEIQKEISSTLEKI
                     QNFVAEIHRNKIQPQKAASFRNVLLLGIGGSALGPQFVLGALGSGTDRMQFYFLDNTD
                     PDGIDQVMASIGKGLGETLTLVISKSGGTKETRNAMLEVRDVYEKNGLDFTKHAVAVT
                     GANSQLDQLAAQENWLDRFPMWDWVGGRTSVFSAVGLLPAALQGLNIYEFIAGAKTMD
                     EITRSPEVSNNPAALLAWMWHHAGRGQGEKAMVLLPYKDRLLLFSRYLQQLVMESIGK
                     E"
BASE COUNT        10896 a         7476 c         8024 g         9600 t
ORIGIN      
        1 gaggaagaag agtctcttca gttactggga caacaaattc gtgaagcacg aattacaaaa
       61 aatctttcac tagaaagcgt ttccggacat ttacatattc ccctcaacac tctacagtct
      121 atcgaagacg ggtgtaaggg caatgtacta cctccagtat ttttacgtgg tttggtccgc
      181 tcatactgcc tgtttttggg gttggaaaat acaggcatta ttgacaaaac agaccggctt
      241 ttgaaatccg atgatgaaaa tgaacagttg aacctaaaat cgcttaggcc ggtttatgaa
      301 aacagggaat caaagccaat tggaataatt ttgacagttt tggtccttat tgttggagga
      361 ttctttgtct attccttcta ctttttgcag acgccttttt ctttcaacgg agacaatgaa
      421 acaataacag aacccctggt agttgaagtt gaacctataa tcgaaccggt acagaaaagt
      481 actccagcca ttcaggaatc tgtcagtcct gcagagccat ctcctgaaat acctgaagta
      541 tcaacggaga ttactcttgt agaaaaaaaa caaactgaga atacacaaat aattcaaccg
      601 gtagcggttc aaaacactgt ggagccactt gttcttgagg tggaagcctc tgaagacact
      661 tggattagta ttgcagtaga taaaaaagaa actaaagatg ttctcctgga agctgacaaa
      721 attcagcagt gggaagcaaa aaaacagtat ctgctgacac tgggaaatac tcatgctgta
      781 cgggttttgt tgaatggaag agaaatagaa actaaccgcg ctcatcagtt gctcacagac
      841 tgggtaatcg acgcaagttt gttaccgtag atttcttgaa agccaaatat tacatgcagc
      901 aattccgagt gtttgaacac ggtaagcgct accgtccgct tagtcagcac tatctggagc
      961 gatttggcga aaaagtattt aaggtgagta tttctgtcgc agaatcatgt ccaaacagag
     1021 aaggtcacaa tggggcggca gtctgcatct tttgcgatga atggggatcc gccgcctatc
     1081 accagcaacg agaaaaacct gtccgcgaac aaatccaaat taaccgagaa gctatccgga
     1141 agcgttatcg agccgaaaaa tttttgatct attttcaagc ttacaccaac acatttggac
     1201 gtttcaggga attggaatac ttttacaagg aggctttgaa ggaaaaggat gtggtcggaa
     1261 ttgtagtggg aacccgtcct gattgccttc ccaaaagagt tttgcagaaa ttttcagaaa
     1321 tcgcgcgaga gaactatctg tccgttgaac tgggtgtaca aacctttgac gatgcccagc
     1381 tgcagtttct ttcacgtgga catgacagcg cttcctcttt aaaggcaatc cgtaaattaa
     1441 aaacacttcc ggacattaat ttgtgtgtgc atttgatgtt tgggcttccg gacgaaactg
     1501 atgcacaaat tcgcaaaact gcagaaattc tgtctgaaca aaaagttgac ggagtaaaat
     1561 tacacaacct gcatgttctc cgcaataccc cgctggaaaa gttgtatcgc gaatcccatt
     1621 ttaaaccggt tgatctggaa gaatatagtc gtaaagttgc attgtttctt gaaaatcttt
     1681 ctccaaaaat tgctgttcac cgcttggctg cagttgcttc gcgctgggat gagttagtcg
     1741 caccgcaatg gacccgcgaa aaaatgaaaa ccactcaatt tattgatgat tttctggctg
     1801 caaaagaaac atggcaggga cgctgttttg aggtttaagg tttgaaattt gggctttgag
     1861 aatataagta acgtccctga ttttttctgc attgaaattt ttattaaata aatatttaaa
     1921 cttttgggaa aagtagtaac agcttgtcat tcacatgaag cttcgtctgg atgacagata
     1981 agtgtggcat tcgaactttt taccacttaa tcctttcata taaaagaaac ttggttgctg
     2041 aaaaagatac tgttttgaaa aattcagata ataaagtggc ttatgaatta aattatgaaa
     2101 ataatttgga caaggcaatt gctgatgatt cggaccgagc tttaataaag cggatcctgg
     2161 aactgctgga ggatgcaaag gccgaagaaa ttgttttgat tgatgcacag catcgttcaa
     2221 gtctggcgga ttatctgctg atttgtgaag gtcgttcgca gttgcactgc agagggattg
     2281 cagaaaatgt agaacataat ttgaagcggg aaggggaggc ttctttaggc attgaaggtg
     2341 agcgtgaagg gaactgggta cttttagatt atggaaatat tatcctccat gttttccatc
     2401 cggaaatacg taagtattac agattagaag agctttatga gcaggggcca ggccaaaaca
     2461 gcaccaaaat gtccatgacc tcagaataat ttaaggtcga aaaatgtcaa acaaagaaaa
     2521 accggaatgg ttggaggaac tggagaaagc ccccctgacc tcacgattac gcagaaaaga
     2581 atccctgaaa cgcttcaata catggcgtat cggcggagtt gcagagtgtt tgattgacgt
     2641 tgtcaatgct gaagacctga gcctcttact acccttcata agcaaacacc gcattccctg
     2701 gttcatcctt ggcaaaggtt ccaacctgtt tattcccgat actgcctggc ccggaataat
     2761 tttgcattta agcggagagt tcaaaagttg ggttcctctc gaagaaaata attcctccgc
     2821 ctgcaaaaat agagtcactg cgggagcggc actggccgat gtgacatttg ctcaacgctg
     2881 tgtggctcag ggatggggag gtatggaatt cttgatcggc attcccggaa cgattggcgg
     2941 cgctgttgcc atgaatgccg gtgcacacgg tggagaaact gcagattttt tgcaggaagc
     3001 agagtggatg gacatggaag ggaatttaca tacctcagcc agacaaaacc tggaattccg
     3061 ctaccgatat tctgatctta tgggaaatta tggacggatt attaccagtg cagtttttga
     3121 attccaggaa tctgatccgg aaactgtaga aaaaaaactg cttgaatgtc aacagttccg
     3181 gatggaaaaa caaccttaca atcaacccag ctgtggttca gtcttcaaaa atcctcctgg
     3241 agattttgct gcccgcttaa tagaagcctc cggattgaaa ggtaaaatca ggggtggtgc
     3301 tcaaatcagt ccaatacatg ctaattttat tgttaacctt ggaaacgcct cttcaacaga
     3361 tattttatcg ttgattgata ccgtccggga aacagttttc agcaagcatt ccattaattt
     3421 ggagcctgag gttcaaatcc tgcagagttc agtatacgga taactttcag cagtttactg
     3481 taaacggatt gctaacatcc gttttcaatc ctgtctgaaa accatatact gccaaacatt
     3541 tactgaaagc cttgttttcc atgcaggatc tcaaacgaca atcaaatcga cgccctgttg
     3601 tgcgaaagct ccggacttca tcaatgagag tccggaaaaa tcacagcaat ccaatcccgg
     3661 gtctttccgt taaactccgc aaacccaata ccgaacctgg ttacagcaat tcctggctgc
     3721 cgccgtattt tgtcgaaaat gaacccgcat ttttgcgtat gaaaatgcgc tggctgattc
     3781 aaaaatttat tggattctgt ggactgattt ttattttatg gctggttgga ggcagtgttt
     3841 gggttggtca atggtttttt caacaaccac tttcaagggt caatatcaaa ggtaacctgt
     3901 tgctgaaaga tgccgatgtg ctttttgcaa gcggcctgcg tcaaggacaa cgtttgatcg
     3961 atctcaatcc atatcaggtt gcagcacgtc ttcagacaca tcccattgta caaaaaaccg
     4021 acatacgtag aaaatttccg gacgaaattc acctgattat aagtgaacat cagcctgttg
     4081 ctttgcttca gctttcacaa cagacgaacg ttttttcatc tgcgtcctta tcgaaaacaa
     4141 agtacgtcct cattggaaac aatcagcgcc tgctcaaact attaaccttt gatgaactgc
     4201 gggactcacc gtataaaaac ctgccgctga tcaacggact gacagtaccg tccgtaacac
     4261 ttggttccag tttagattca ccggttcttg aaagaggttt aagatttctg gaaacatttc
     4321 aaacaatggc tgcagctcaa aaagctgaac tgcaaaatac acagataaaa cttgaaggtc
     4381 aattcagaat agttgactgg tcttctcagc caattcacat tgatatttct gatccgctaa
     4441 acctgaaaat taactggcca ctgaattcat ctgagcttca acctggttct ccggagcctt
     4501 tgcgtaccgt tccgctgacg atccaaatgg gaagtcgtga tttcggcgaa cgcttacgca
     4561 ccttccaaaa catttatcct gtattgggta aacagcatcc gggattaaaa agtattgacc
     4621 tgcgctacaa aaatagggta atgctagtac cctgatctaa attcaaaaca aaaatactgg
     4681 aacgtagaca ttttgtctgc cggcatttaa tggagcatga caatggcctt gttggatgaa
     4741 ctggtgcagg aagaagattt ggacccggaa gtgatggggc agaccagcag cgctttcaag
     4801 aacctcccgg agcttgagaa aataaggcgt ctgattcaga tggatttgct ggaagaacct
     4861 caccttctcc ggaatatgtc agaccgttat aacctcccgc tgctttcaac aacacttgat
     4921 gaagtccaaa attacgaaaa ccttcctttg tcaaagcaac tttatgaacg caccggcatt
     4981 ttaccgatgc gtctgggatc aaagtgtgcc tgcctgttgt cagttcagtc aaactggttg
     5041 aagatgaatc aggttgcctt tcatctcggc gaaccgattt attggtattt agcacaacag
     5101 cagcaaattg aagcactgct tgaaacttcc catttgcaga ttgaagatgc tgatatttct
     5161 gcaaccgaca gaattcatca tttgtttgaa gaagctgttt cccgaagagg atcagatatt
     5221 catcttgaac ctgaaaaaaa tcagctgaga gtccgattca ggattgacgg ctacctccag
     5281 gactccgctc caatgccgat gagtttccac ccgcttattt tctcacgcat taaattattg
     5341 gccggaatgg acatcgctgt caggcgccgt cctcaagacg gacactacac atacagctcc
     5401 aacagtaatc gcatttttga cgttagagtt tccactttgc cgggtcaatt tgggggaaaa
     5461 ctggtgcttc ggcttttgga tcagacaccg gtacaacaca aattggaagc actcggtttt
     5521 ttcaaaaatg acttggaaat gctttatcag gcaagccagg ctccaagcgg cttgatacta
     5581 atggttgggc cgaccggcag cggcaaaaca actacactct atgcaatcct caatgcactg
     5641 aattcacaag aaaagaatat acttacaatc gaaaatcctg ttgaatatca catagaaggc
     5701 atcacccagg tttcagtcaa accggaacaa ggtttgggtt ttgcggatac actaagagcg
     5761 gcactgcgtc aggatccgga tgttctgttg atcggagaaa taagagatga agagactgca
     5821 aacattgctg tcaaagctgc gcttacggga catttggtac tctcaacact tcacagcagc
     5881 gatgctgtaa ctgcaattca gcgtctgttg aacctcggga ttgctccgga tttattagca
     5941 gaaacactga cagtaatcgt ttcccaaaga ttagtgcgcc gcctctgttc acaccagaac
     6001 ggaaacaagt caaatcctca tacttcggac aaaaactgct tgaggtgcag gggtacgggc
     6061 tactccggcc gcattcctgt ctacgaaatt ctgaaggtga attctctggt acgggaccgg
     6121 attcaggctg gtgacacagg aaggaaattg atacgcccag aacctgaact ttactttcac
     6181 acaatggaac aaactgctca acgcctgacc aaagagggtt tgactgactg gaaagaagta
     6241 cagccgctgt tactgaatgt ttaagtattg aaagaagttt tgagacttta ggctataata
     6301 ataattgaag atgttaatca gttctttctg gacagagatc ttagatttgt ggaaaagcaa
     6361 ttgaatccct tgatcaaaca acttcatgtt actccctcat agctgcaaca ccaaatcacg
     6421 ttggcggaaa caatgcacta actctaaacc tgtctcgtcg ctgaaagcct ttcacaacat
     6481 aactactgtg tgagattaaa tatgcataac ttttgcacga ttttggcagg aaagtctttg
     6541 gggggtgttt tttggcaaaa taaacaaact ctatccgtaa gagccgatta actgaaacct
     6601 attgtatatg ttagtaccga tcgtgctggt gcaggaagaa tttgaatata actggtctgt
     6661 tacggcaacc gcggcagact gatgttattc ggcagatctg tttaaagcta ttctcaaatt
     6721 ggttgtagtt aagttattat agtgttatta ttaagagtta ttttcggcca ttttctatgt
     6781 aaatgtgtgt atgaaaaact tttcaatcct tctgcttaaa gatagtaact ttggagtaag
     6841 ccagtatcat tttggggcaa agtttatgtt ttttttgttg accttgatat tggttggcgc
     6901 tggagcttta ggttggaaat ttttcgacca gcaccagaca atcttgaagc aggaccagtt
     6961 acttgaaaag caggaaaata ctcagatagc atttcaacag cggattgatc tctatgacgg
     7021 ccgggaatca agaatttctt ttcttgaaaa ttatgttgaa gaattgaagc agtcttcaca
     7081 taacagtgac gtaatgttta agaaacattt ggcactattc cgttccaaca cacataagtt
     7141 ggatgaattg catgattaca tgtgccagac attggaggct caatgtacac caaaactcaa
     7201 ggatcaaaca aatcctcaac agaccgtgca gtggctggat cagatatatc aggattttct
     7261 gctctttaat cagtctttgc aggaatttgc ttcaaatcgt agaagttttg aagaacaggc
     7321 aaataccatt cagcagttac gaggacaact cgttgaaaca gaacaggatt tgtctgaaca
     7381 tatggagttc ctcaaagtta atcaggcgac ggtcatccgg ctaacaaaaa agatcagtaa
     7441 aactactgga ataagactca gcaaaaccgg aaaaaccaaa cgagtaaaag ctaaaactgg
     7501 gcgcggaggt cctacaattt tggatagctt gtcactcgac tcaccgcaaa ctttgttaag
     7561 ttccggaagt ttgcgtagtt ttctttacaa taattctgat aactactacg aaaccgttgc
     7621 ccaactggag gatttaagcc agttgattga acgaaatcaa agtttctgga ggcatactcc
     7681 gaccgtgatt ccagttcgaa gcagagcatt atctgatcgt tatggaatgc gaaaggatcc
     7741 gttcaccaag aaacgtgagt atcatgctgg cattgatttc agagcaaaaa gaggcacccc
     7801 agttcacgct cctgcagatg gcactgtccg gattgcagac cgcaaatcag gatttggact
     7861 gcttgtggaa ctacaacatg gaagaggttt ctttcccgga aagaaaaatt ctgttcgcta
     7921 cagaacacgt tttgctcatc tttctaaaat aaaggtaaga cgcggtcaga aaattaaacg
     7981 tggagacttg cttggattag taggaagcac tggacgcagt actggtccac atttacacta
     8041 tgaaataatt attaacggac gacgtacaaa ccctctgttt ccaatttcca ggtttaatcc
     8101 ggaccagcgt ctttatcgaa attgagtgca aagctgaaaa attaacatgc cgaaccaaaa
     8161 tactgcagca aacaaaggac ttggattcct gggtgaggat ctatatgtag agggtaccat
     8221 ccactcacaa aagaaaatgg ttgtgagtgg aacagtagat ggatccgtat tttgcgacca
     8281 ggagattgtt gtttcggaaa ctggacaagt caaaggaaag attgaaggta atatagtact
     8341 ggttgcagga aaaatcaatg gtgatttgct ggtccatgaa cgccttgagg tgaatgtaac
     8401 tgcaaatatt aaaggtgagg tacaagttcc ttccggaaaa ctattaattc aggaaggagc
     8461 taaactggat gctcaatgca ccattctcta cgacaaaact taagacagac aagcatccac
     8521 ctgatttact ttgagcaacg aaacttctgt gtccagcaga tgattatttt taattttcaa
     8581 taatgcaata cccaacttca aaaaatagtc ttaaacagca aaacaagcca gttgaacagt
     8641 caaaactgct taagaatcat gacagaattc tgtatgttag cggactgttt tctccaccaa
     8701 aggtacaaga gactcctgtt gttggcatgg caaaagaaac tgaagagtgg cgctcgttgt
     8761 tctcactggt cttaattgca gggtttggtg tggttatgct cataatctca gtgattggga
     8821 gttatggaat attagccacc agcagactgg aagcacatga acaaatgctt cttcaaaagc
     8881 ttgaatcgat gaaggttgag aaaactttga tgcaagaaga agtttttgca ctgaaaacca
     8941 gtcaggaata tattgaattg attgcacgca aagagttggg cttagtgcat tccaccgagc
     9001 tgatttatta tctccctcct tcagccaaag attcctcccg caccgatttt taattaatct
     9061 aaaaaatgcg gtcttcaagt ttttcaatag attgttgaat gacttccgga ataggggttg
     9121 tctgattttt ttgatagttg tagcacacca caattccagt accttctgca accggatccg
     9181 catgcttctc actgacgagc agatactcca ttgtgaaacg tttttcgtgc aattctgtta
     9241 tttttgcacc cgcaaataca gtgtccggat aagtgagggg aatccgatag cggcaggaag
     9301 tggagcctaa tattggacca atgcccattt tttcaattat tttcagccag cctaaagtct
     9361 caaaatactg gattcgggca ctctcaaaat aacgaaaata gatcacatta tttacgtgct
     9421 gaaatgaatc catatctccc caaattactg gaatttttgt gacaacagga aaagttttta
     9481 agagttcttt cataaatttt ctcaatgagt gaaattaata aacaaaaaga gggcctgccc
     9541 caaaaaatag ccttgattat ctgcaatggt aatccgcctc cggaaaagct gttgcaccag
     9601 ttatggcaag aagcagatta tcgggtagca gccgatgggg gtgcaaatca gctgtactgc
     9661 tataatctga tcccggatgc agtggttggt gattttgatt caattaagcc ggaagtccgg
     9721 aagaaattgc caaactctaa gctatttcat gttaaagaac aagacaccaa cgatgccgac
     9781 aaagcagttc gacattgctt gaaacgcgaa tgcacagaaa tacacattct tggagcagaa
     9841 ggtagtcgta atgatcagtt cctttcaagt ctggaaattc tatttaaata caccccaaat
     9901 gtacgcctca ttttgtggac tccgctggaa cgcatggagt ttattcttga caactggaaa
     9961 gagaatcttc cgccaggaac aattctttct ctgctgccgc ttttcggcgg tgctcaaggt
    10021 gtcgtcacac acggattaga attttctctg gacggtcatg atttgcttcc aggaaaaaca
    10081 ccttccggag tctcaaatct tgtaatcagc aatcctgtat cagtaactct gaaaaaaggt
    10141 cagctgttac ttgttgttca gcacaccaca aattattcat aacactctga atcagataaa
    10201 agtcagaata actgtcaaag ttagaatgct gtaaagcgtt gaaaagagta ccgtcgttgt
    10261 aacaaattca ggaagcagtt tgtattccaa tgcaatgatt gaagccagta cagctgcggg
    10321 cattgctgcc tgaagaatgc cggtactgcg ttccagacct tccagcccaa aatagggtac
    10381 aataagcagc gccaaaacag gacctcctat caaacgtact gtacttgcag caaaaacgtt
    10441 gaagttaatc tttatttttg gaatctctcc catttgaacc ccaagtgtaa tcagcatcac
    10501 agggatcatt gcctgcccca agagtccact caaacgtgac agaaaaattg gtacttctac
    10561 atcagtaata ttgaaaacaa gagccggaat cagggctatt aaagccggtg ttttaaaaac
    10621 agaaaagaca gctgtcacac ctccgctgcg ggcccaactt gccacaccaa cacaaattac
    10681 aaaagaaata aagacagtcg ccacaaaata aacagtggca aaggtccggg ctgtctctcc
    10741 caatcgaaac acaattaacg gcaggccaaa attaccaaca ttgccaaacg ttgcaatcag
    10801 cacgaaagcg gctgtaattt ccctggactg gcgcagggct ttaccaacca aaaaacccag
    10861 caaagcaacc gcaatttggg caacaagaat aaagctgagc atctgtaaag ccaattcaga
    10921 atcaattttc gcctcactga taatattaaa tacaaatgcc ggaatgaaca caaaataggc
    10981 tgttcttgaa agtgaacgtg catcaatttt tagacgtggg ccaacaaaat aacctataac
    11041 aacaagaata aaaactggtg tgacaacatt gaggaaaact aagaagatcc caccaatatt
    11101 ttccataaag ataaaactgc tcattctatg gctaacaaat cattaagtgt aggttatgtt
    11161 aagatcaagg atttaagtta ctattttatc cggaagttcg cattcttggg aaatcacaca
    11221 atgtccacaa tcaggttttc gtgccctgca gacataacgt ccatgcagaa ttaacaagtg
    11281 atgtgcgtct tttttccatt ttgcaggaac tactgacatc aatttttgtt caacttttat
    11341 gacagtttct cctggtgcaa gcccggtacg gtttgaaagc cgaaagatgt gagtatcaac
    11401 tgcaatcaca ggcaccccaa atgcagtgtt taaaatcaca ttcgccgttt ttcgtcccac
    11461 tcccggaagt gcctctaaat cctttctttt gtccggaacc tcccctttgt gcaacttcac
    11521 cagttgtcca caagttgcag caatattttt tgactttgtc ggtgcaagtc caatactccg
    11581 aatgtacgca aatacttttt ctgaaccagc ctcaaacatt tcttcaggcg taggaaatgc
    11641 ggaaaataat tccggagtaa ctttgtttac agaaacgtct gtagcctgcg cactgagaat
    11701 tacggaaatc aataactcga aaggattccg gtagcaaagc tccgtcttcg gattcggaat
    11761 tgagtcgctc aacttccgga aaagactagc tatctttgca gatttcatgg ataaaacatc
    11821 tcaaatttgt ttccttaact gcacattcag ttgcctgtct gcaggttttc actcataact
    11881 tgttttagga accgttccgt atttatttcc aaaactgcag acaatctatc acaaatgtcg
    11941 aacaatgcca gtcatgagtt aaattgacaa ttaacactaa taaactgaca atggacaaca
    12001 atccaataat gaaaccgtcc actatggtct ttcaaaatta ttgaaattaa aaaccgatta
    12061 caggtttagc acatgaaaca acttgatttt ctacctctcc ggatctcaat tctggtggtt
    12121 tctgattctc gaactgaaga aaccgatact tccgggaaaa tacttactga acgtctcaca
    12181 gaagaagggc acattctggc ggaaaaatgc attgtgcctg atgatattta tcagattcgt
    12241 gcagtactgt cccgctggat tgttgatccg gcagtagaca ttatccttag caccggtggc
    12301 accggactca ccggacgtga cggcactcct gaagcagtat tccctttatt tgacaaagta
    12361 atcgagggct tcggtgaatt gttccgccgg atatcatatg atgaaatctc cacctcaact
    12421 atccagtcaa gagtcacagc tggagttgcc aacggaactt atattttttg tttgccgggt
    12481 tcttccggag cttgtcgaac aggttgggac aaaatactag caactcaact tgatatccgc
    12541 agtcgaccct gtaacttcgc agaattaatt ccaagattga ctgaaaaata gtaaataggc
    12601 tcagttgtca gttatcaagc ttaacacttt taaaatactg aaagacttct ttcaatgagt
    12661 caacagctca ctctgtagat tctaattttc tgcttctgat gcaataaact cctgtaccac
    12721 aggaacaagt cgctccatcc cttcttccat aggacagaca accggtaagc ccaaacgctc
    12781 ttccagtttt tgctgttctg actccagttc ttttttagtg agttcgtaac tgttcagtgt
    12841 tatagccaaa gtttgagaac catataaacg aatcagttcc aattcttcct caatcggcgg
    12901 catcggccaa agttttcgat catcatcagt caggtaatat ttctgctttg gcgcatattg
    12961 taaaatcaca cctttggcca gtgcactgca aagatattct gaaccgcatg gtccggaagg
    13021 attgcgtaaa gaagactgac cttcaagcag catcaagtca ggattttcct cctgatcaca
    13081 acgaaaaatt gcctcttcca gttctccgct gacaaaatca ttgagggttg aatcaagaat
    13141 aaatccatag cgtccacctt gcagtttccc ggtctgcccg gtgtaaatca tttcggtgcg
    13201 aatgccctgc ttacgacagg catctctgag ccaaagactg gaagtgcgct tacccaaggc
    13261 acagtccatt ccaaacatgg caattcttgg agcacggaca ttcaggatat ttcctgtcca
    13321 aaatgcgtga tcctttgcac ttttgggttt tcgaatatca agcaatttgg cctgattttt
    13381 ctcagctctt tgtactaatt caggatcatc gctcagatag tggtgcagac cgtttacaac
    13441 atgcatacca ctggacagtg cttccagtaa ctctatacga agtccctcgg ggattacccc
    13501 gccttgagtt gcaatcccaa taattgcgta tacaccacgt ccgctttctt ccaaagccag
    13561 tttgtttctt gaacttctgg ctcttaaaaa ttcattaatg gttgcatgaa ttgggatgtc
    13621 tagcacctga ccgtcaacga gttttcctgc atcctgtccg gcaaagtttt gatcgatgac
    13681 tgcaagaatt tcatattgtt ctgaattacg aatcagacca aatgcagtct ttgcgttatg
    13741 agaatcaaac aatccactgg tgagaataat tgcttcttct ttcaagggaa atctttttaa
    13801 ataagagtta tgatggtttc gttataagtc ttaaatccac aatttgtcgt ttcaaccgta
    13861 gggataaaac tcaatgatat tagcgtagta gcatatgcaa gatttctcac ttcgctcaaa
    13921 atgacaagga tttagaactt attacaggtt catcagttat agttatttga ttatagtatt
    13981 atcggacact ccgtttattg cgcggcctgt ttgtcatcat acttcaagat tttttttagt
    14041 aaaagtgcat ctcccaaagg atttataaaa taattttttc ggatgcctgt ttcttgataa
    14101 ccttgtttgt aatacaatgc agttgcagct ttgtttgcag aattgacttc gagccagagt
    14161 gattccagat tagctgattt cgctactatt tccaactgct ctaacataaa ttgagccaac
    14221 ccttgtttcc tccaatccgg cagaactgct aatctgagga tttgcagttc cggaggtact
    14281 gactggaaag acagcattcc tacttgagct gcagttggaa cttgcagcag ccaatttgag
    14341 aaagtaggct gctcctgcat ttcccgataa tcttgttcac tccatgcagc actgaagcag
    14401 agggagtcca ttttcatcat ttcttttggc gaaggctcca aacaaaaact gatgccctgg
    14461 atatgatgag ctgaagatgg aatcatcact tcttctacta ttttcttgaa aaataacttg
    14521 taaactacca gaagattagc tataaatata ggtttaattt agaatattat taaagagaag
    14581 aggaatctgt ctgtcagggg atgaggacaa cagagccgat tctttatgat tcacttcaga
    14641 gccagttcgt ccgattacat cccgcggaca tttaaccaag ccatcataag gagattttat
    14701 ggctgtcatt tcaatgaagc aatttttagc agctggagtc cacttcgggc accagacaaa
    14761 acgttggcat cccaacatgg cgccgtacat ctacggcata aaaaacggtg ttcacgtcat
    14821 cgatctgcgt cagacactga ccaagctcaa agaagcttac gaatacgtta aagcctctgc
    14881 agaagaagga cgggtaccac tattcgtcgg cacaaaaatc caaatacaac gtatcgttaa
    14941 agaagaagcc gaccggtgtg ggtgccccta catcaatttt cgctggctgg gaggtttgtt
    15001 aacaaacttt ggtactgtaa agcagtcaat cgcacgaatg aaaacctttg aagaagttgc
    15061 tggagaagac ggcagttacg agggtgtgat caaaaaagaa gctctgatga tggagcgaaa
    15121 acgcgtgaag ttagagcgtt cactcggcgg agtacggaat atgaaaagtg taccatcgat
    15181 gcttttcatt acagattgtc ataaagaaca gcttgcattg aaagaggcac aaaagttggg
    15241 aataccagtt gttggtattg ctgatacaaa ttgtgatcct accggtatag acttcgttat
    15301 tcctgggaat gacgattccc cacgtgcagt tgccctttat gcaaacgtaa ttgctactgc
    15361 agtcatggaa ggtcttgctg ctcgaaaaca aactagaata gaatcgatta ctgaagaaaa
    15421 agctgctgct aaaaaagcaa aacctgcagc gaaagcaaag gagaaagaaa ctgatgttgt
    15481 ttctgaggca aaagctgaag aagagaaaaa agctgaagca aaagcaaagg agaaaaaagc
    15541 tgaagaaact cctgccaaaa taaaagagga gaagaccgaa gctgctccgg gaccaaagtc
    15601 tgctgaaaca aaagtggctg ccgaaaaagc aaagcctgaa gtgaaagcaa agnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaagcaaa gcctgaagtg aaagcaaagt
    15781 ctgaagcgaa agcaaaggag aaaaaagctg aggttgttac cgaagccaaa tctgcagtgg
    15841 aaaaaacacc agctaaaaaa actgctgcta ctgcaaaaaa agccgaaact gcttccgggg
    15901 aaaaagttcc cgcagcaaaa gcgactaagg ctaaaaaatt ggaaacaaaa aaataaacag
    15961 tacctaccag ataatctttc cggatttaca acttgtaagt ccggaaacca cataaaaact
    16021 taataagcat tggaagtata aacaagatgg cagcgattac agcagcagca gtaaaagagt
    16081 tacgggaaat caccggagtt gcaatgatgg attgcaagaa ggccctcgtt gaagcagacg
    16141 gagatctgga agcagcacaa gattatctgc ggaaaaaagg acaggcaaaa gccctgaaaa
    16201 aatcttcacg ggaaaccagt gaaggtgcgg taagctttac agtcagtaca gatgggaaac
    16261 gtgccggttt ggtaaaagtt gcctgcgaaa ctgattttgt agcacgtaac gacatatttc
    16321 aggattttat cagcaaactt gcaaatcaag tgtctgagca aggcggggaa aatgttttac
    16381 aacagacatt agtcgaaggt gaaggcagtg ttgaaagttt gttgacagaa aaaattgctg
    16441 aattaggtga gaacatgcag attcttgact gcagaaaaat ggaaataagt aatggtgttc
    16501 atggaggata tatccacagc aatggcaaga tcggagttgc ggtaccactg gaaacggaca
    16561 aaccttgtga cgatgaacgc ctgaagacat tggcccgtga cattgccatg cacattgcag
    16621 catttcctgc agaagcagta aatgctgatc aggtttcaaa agaggtgctt gaaaaagaaa
    16681 aagaggtttt tacggcccag gcacgagaat ccggaaaacc tgaaaatatc attgaaaaaa
    16741 tgattgaagg acggttaaaa aaattcctca aagaaatttg tgttgaaagt caaccttttg
    16801 tcaaagatcc gcaactttct gtgagtcaat tggttgaaaa tactgcaaag gaacttggag
    16861 ttaaaattaa cttcaacagc tttgctaaat tccagttcta gactacctgt atgagtccta
    16921 aatacaaacg tattctactt aaactgagcg gagaaactct gggtgggaaa cgcggtaccg
    16981 ggtttgatta tgatactatt agggcactgg ctgaaagtgt aatctccatt cacaaccttg
    17041 gtgtagaagt tgggattgtg attggaggag gaaacatttt tcgtggatcc aaatctgctg
    17101 aaggtaatgt tggccgagtt gccggagatc atatgggaat gctggctaca gtgatcaaca
    17161 gtatctgcct gcaggaaatg ctggaacagc gaggtatttt aactcgcgtc ttatcagcca
    17221 ttgagctgaa cgcaattgcg gaaccttaca tcaaacgcag agctgaccgg catttggaaa
    17281 aaaaacgagt agtgattttt gccgccggaa cgggaaatcc attttttact acagatacag
    17341 ctgcagtatt acgtgcttct gaggtgggtg ccgaaattgt cataaaagca acaaaaattg
    17401 atggagttta tgacaaagat cccaagctcc actctgatgc ggttcgttac gatgtgttaa
    17461 attacgacga agttctcaaa aaacatctga aagtgatgga cgcaacggcc attgcttttt
    17521 gcagagataa tcaactcccc attttggtcc tggactacca ggaacctgac gcaatcctca
    17581 gggctgtttg tggagaaacc gttggaacac tgatcaagca aagaggctca tgactatgga
    17641 aaacacacag gaagttgaag aaatggttga tcataagatg gatattacca ttgagagtct
    17701 taaagtggat ctcagcggta ttcatgcagg acgtgtttca cctgcaatgt tagatgcagt
    17761 caaggttgaa tattacggaa atccaactcc tgttaatcag gtggcaaaca tatctactcc
    17821 tgaaccacag atgttggcaa tcagtccctg ggaaaaaact atgatcaaag aaattgagcg
    17881 tagtttgcag gccgcaaacc ttggtttcag tatctccaat gatggaaata tcattcgtgc
    17941 ggttactcca cctttcactg aagagagacg aaaggattac gtaaaacaga tcaaaaaaat
    18001 tggagaggac tcgaaaattg cagtacgcaa tgttcgcaga gaagggaatg acaacttgaa
    18061 acaaatggaa aaggataagc tcatttcaca ggacgaagaa aaagtggcgc aggatcacgt
    18121 ccagaaagtt acagataagc atactaacat ggtagatgag ttagtcactg caaaggaaaa
    18181 agaacttatg acattttagg actgaaatgc tggctgcact tctgcattca atttggcaat
    18241 atcagatttt taagtctgtc gtttttcgcg gtggtatggc ttttttgact gcctattttt
    18301 taatagtgtg ggtgatgccg agtgtaatca gggctttcag gaaacgagga attacttctg
    18361 attttatcac aactcctgct gatataaagc cttacacggg agcacctccc atcatgggcg
    18421 gcggcgtttt gattatgggc attttgcttg gagcattact ctggtgcaat ctgaatcaat
    18481 acgttctggc tctgttggtg atcatgcttt cgtttggcat tatcggagca gttgacgata
    18541 tagcaaaagt catcaacaag cggcgcgttg agtccggaca tgaaaccaga aaagcattta
    18601 gtgaaaaagc agatggaatt agcggacgat ggcgactgac tgctcaattt gctgtttcac
    18661 tgctggtagt taccggttta tatttgtttg tcgatattga tggacattta gtagtaccac
    18721 tcgtaccgct gaaaacagca tatccttact tgccaaaata tctttttatt ccattcatga
    18781 ccatcatcat cgttggtgga gcaaatgcag ttaacttgac agacggcatg gactctctgg
    18841 caactgtacc cttgatgact tgtgccatgt ttgtcggact tgtggcctac ataggaggag
    18901 acccagaatt tgcggcaagg ctgaagatcc caagtctttc acatgatatc aaggaattag
    18961 cagttctctc tgccctggtc attagtgcag gtgttgcttt tctaaaatac aattctccgc
    19021 cagcattgat ttacatgggc gacttggggg cacttgcatt gggcagcatg gtttcagcta
    19081 tgtttatttt tgtaaaagca gaattatttt taccgattgt aggtggagtt tttgtatttt
    19141 cagtactttc ctctatcatt caacgtacat ttttcaggtt catagtgtgg cggaaaggca
    19201 ggcctactgc tgagcgatat cgcttttttt tacgctcacc ataccatcat catcttcaac
    19261 gcctgtggac ttattctgaa aagaaacaag aagttgtctc tgtttggata atgttgcaga
    19321 ataaacttgg aatagatcct gttcctgaag agaataagct gttaaatcct caggaagtta
    19381 acagccgcgt tatttggcac atgcatctca aatcaatttg gctgtttgta tttacgctga
    19441 ttatttattt taaaatccgc tgatgtatct ttgtcagaaa gaaaatctgg aacaattaca
    19501 tctgctcaac agaagagttt aactgttaat aaaactacag aaactgccat gaattttgaa
    19561 ggccagattg cacttataac tggagctgga aggggtattg gtaaaactat tgccttaaaa
    19621 ttagcagggt ccggagcaga tatcgtgttg gcggatatga gtccggatgt tgcggaagtg
    19681 cgtggagaag tggaagcatt gggccgtaaa tgcatgactt ttgaggtaga tgtaacaaat
    19741 attgaaacca ttgacgcgat ggttaaaaat ataaccgatg aactgagtgg aattcatatt
    19801 ttagtcaaca atgcaggaat aacgcaggac aatctgtttc tgcgcatgaa atcggagcag
    19861 tggtcaaaag taattgatgt caatctcaat ggagtgttca atgtgaccaa agcagtgatc
    19921 cggctaatgg taaagcagcg tactgggaaa attattaata tttcttctgt ggtcggcttt
    19981 actggaaatc cgggacaagt caattatagt tcaactaaat ctgctctggt tggttttacc
    20041 aagtcactgg cccgtgaagt tggtgtccgg ggaattacag tcaatgctgt tgccccggga
    20101 tttatagata cagcaatgac gcaagctctc aatgaaagtc agcaggaagt aatcctgcag
    20161 caaattcctt tgggccggat gggagatgca gaagatattg ctaatgcagt tgcttttttg
    20221 gcctcaaaag aggcttcgta cattaccgga actattctgc atgtgaatgg aggaatgtac
    20281 taaacattag tacttgcaaa aacagggttg attttttttc atatcttaca aaaaataaac
    20341 ctcgtttaat taatttttca aacctttaat caaaatatat aatggaagaa atacaagcta
    20401 aggtcatcca aatcataagt gagcaattag gtaaggatga atcagaaatc aaaatgagtt
    20461 cacatttcat tgaagattta gatgcagact cattggatac tgtagaactg gtgatggctc
    20521 tggaggagga gtttgaggta gatattcctg atgaggctgc agaaaaaatt accaccgttc
    20581 agtctgcagt ggaattcata gctgaatcca aagcctgagt aacgataaaa aactttaaat
    20641 gaagacgtgg cacagttaac aaataatcgt gcttgttatc atttcacgta atcaatctga
    20701 aagagcatgg gaaaccgcgt tgtagtgacg ggactcgggt gcatgtcccc tctcggaaat
    20761 gatgtctcct cttcctggga ggcctgcacc actggacaat ctggaataga ccggatcact
    20821 cattttgatc ctgaattatt ccgttgtcag attgcaggtg aaataaaaaa tttccagctg
    20881 cctgaaatca ttccgatcaa agaagccaaa aaaatggatc tgttcattca gtattcaatt
    20941 ggtgccacac aggaagcttt gcttgacgca aacttggaga tcactgaaga tcttgaagaa
    21001 gaagtaggtg tttcaatggg cgtcggaatt ggtggactgg gctgtataga acgatactcc
    21061 aaaattattg aagagcacgg ccccaaacgt gtcagcccgt tttttatccc gatgacttta
    21121 agtaatttgg caccgggata tgtctcactt ctttttcgag ccaaaagtta caccgcctgc
    21181 accgtttcag cttgtgcatc ttccaatcac gcaattggca ctgcagcccg aactattgaa
    21241 cgtggtgatg caaaagttgt aatttcaggc ggtgcggaat caagtgtgac accactcggt
    21301 attgcaggtt ttgcgtcaat gcatgctcta agtaccagaa atgatgatcc acagaaagca
    21361 agccggcctt tcgacaaaga ccgtgacggg ttcgtcatgg gcgaaggttc agggatgcta
    21421 attcttgaag actacgaatt tgccaaagca cggggagcac gtatttactg tgaaataaca
    21481 ggatacggtt tcagttcaga cgcacaccat gtgacatctc cctcatcaga aggccccacg
    21541 cgagccatga aaatgaccct caaagattcc ggtttggctc cagaggaaat tgattatgtc
    21601 aatgctcacg gtacatcaac cccagtcggt gaccttaacg aactaagagc cataaaaatg
    21661 gccttgggag aaaaagctgc ccagcaactt tctatcagtt caaccaaatc catgaccgga
    21721 catcttctcg gagctgcagc agcaattgaa gcaatctttt ccattaaagc tttacaccat
    21781 aattttattc cgccaactat caatatcgaa aatttggatc cggaatgtga tttggacgtg
    21841 acaccgaaca ccggaaagtc aaaagaaatg cgcgttgcaa tgtcaaatgc ctttggtttc
    21901 ggtggtacaa atgcctcaat tatatttcag aaaatagact gattcctttt tagtcagtca
    21961 ataaccggaa catcagacag atcaaaatat gaatctcaaa accaatccta tcctctttta
    22021 aactcaaaaa acatcatgag cagcaccgaa tatcaagtcg accgcagaga gcttcaattc
    22081 gtggttaacg aaactttgaa agccggaaaa ttgtgtgaac ttccggactt tgctgaattt
    22141 gacgaagaga tgtttacaat gattatcaac gaggccagta ctttttcgga acaggtactt
    22201 ggccccctca acgaaaatgg agaccgccag ggttgtagaa tagaaaatgg ctctgtggta
    22261 actccagatg gttttgcaaa tgcctggaaa caacttggcg agggtggttg gttgagcatg
    22321 aatatgcctc ccgaatatgg aggacaagga cttccggaag taatttctat gattgctaaa
    22381 gaaacccaat tggcggcaaa ccagggattt accatcggcg catcactcac atccggttcg
    22441 gcaaacctga tttacacttt tggttcagaa gaacaaaaaa tcaccttttg cgaaaaaatg
    22501 ttcggcggca agtggtccgg atcgatgtgt ctgacagaac ctcaggccgg aagtgcagtt
    22561 ggcgatgctg ccaccagtgc tacaaaacag gaagctggac actacctgat aaaaggtaca
    22621 aaacagttta tcacaaacgg tgaccatgat atggcggaaa acatcatcca tctgctgctg
    22681 gcacgcacca cagatgcccc tgaaggaact aaaggcatca gcctttttat agttcctaaa
    22741 attcgaatgg atggaacacc gaatgatgtg aaggtgatca gcattgaaca taaaatgggg
    22801 atcaacggat caccaacttg cttactctca tttggagata atgcaagttg tcacggctat
    22861 ttgctgaaag ccgaaaatat aggtatggcg caaatgttcc agctgatgaa tgaagcccgc
    22921 ttactcgtcg gtttgcaggg acttgccgga gcaggtgcag ctgtgcaaaa tgcatgggca
    22981 tatgcaaaag aacgtactca gggaccatct gcaattcatc ctggtgaaaa agctgcaatc
    23041 attgagtttc cggatgtgcg tagaatgctg atgcagatga aagccgtaac ggaaggactg
    23101 cgtgccctga tgtacacgac agcatggtac acggacatgg cgcatcatgg tccggatgaa
    23161 tcacgcgaaa aatatcaaga cttgcttgac ctacatattc cagtctgcaa agcattcggt
    23221 actgatcagg gatttgacgt ggcgcggatc ggcattcagg ttttgggtgg tgttggattt
    23281 acacaggatt ttccgcttga gcaaaatgca cgtgaccaga aaatcgcctc gatttatgaa
    23341 ggcacaaatg gaattcaggc tcttgatctt gttagcagaa aatttactgc caaaaaagga
    23401 cagttgctca aagttcttga acaggaattg agctggtttg acaatcatgc tccagaagat
    23461 gaactcgccg gctgggttgc agaatgggaa agttaccgga cattgatgca ggaaagcatt
    23521 gccggtttga aaaaaattgg agaagaacag ggaaaagacg gttatgtcct ttatgcagtc
    23581 aatatgctgg atttgatggg cgatgtactg tgttgttttt atctgctcaa acaggctgat
    23641 tcagctcagc aaaaatggaa agccttgctt gctgaagcaa cttctcaggc agaattgctg
    23701 gaggaaaatg aagaagctca gttttactgg aataagctgc gtaccaccga attttatgtt
    23761 tggagtgttc taccccgtgc tttgtcaaat gcaaaaacca ttaaaaacgc aaacctcgca
    23821 cctctaaatg cttgtttgta gaatccatta tttgcagtaa atctgtcaaa gaattcattt
    23881 ctcagtttaa atacaaacga atcgtccgtt acaccgctct ggcctgattt tctcttgaat
    23941 ctccactttg caacctcaaa tcccaataaa ctaagtgagt tatctgagtt attggaatac
    24001 cggttaactc cattagagct ggatttacag gaaatccaaa ctactgatct ccaggaactg
    24061 gtaaaacata aactgaagca ggcatacgaa cacattcatg ctccagtgat cgttgaagac
    24121 acatcactgt attttaaagc atggaatgaa cttcccggac cactcgtcaa gtggtttata
    24181 gagaaccttg gtttggcggg aatggtaagt gctttatctc aatttaaaga taagtccgct
    24241 caggccgttt gttgtttagg atataccaat aatggtaaat ctatgtattt gtttgaagga
    24301 aaagtgaaag gtgtaatcgt caaaccaaga ggctcactta attttggttg ggacgcaatt
    24361 tttcagcctt cagagcatga gcagactttt ggtgaaatgg atccggaaga taaaaatcgc
    24421 atttcacctc ggggaaaagc ggccaccaaa tttaagcatt tcttaacaca gtcggcaaag
    24481 caaacgtaac atcctcttga actatttcaa gatcacggac actgcttgaa gatgacatct
    24541 tactgatttc tgcaactatg ccctgcacga tatcctcagg cgcggatgct cctgcagtaa
    24601 taccaacgtt ccggacctct tccaaccatt ccggatccaa ttctgctgca gattcaatgc
    24661 ggcgggcctt aactcctgtt gtttccgcaa cttccagtaa acgcaccgaa tttgagctgt
    24721 tctgtgcccc cactaccagt accaaatcaa cttccttact caaagccttc actgcatttt
    24781 ggcgattttg tgtagcataa caaatatcat ctttagccgg accctttatt tccgggaagc
    24841 gctctttaag cgcagtgata atttctgcag tatcatccaa agataaagtg gtttgagtga
    24901 tataacctac ctttttctca tccggaatct tcagatccga tacttcttct actgttccaa
    24961 ctacaaaaat atgttcggga gcttctccaa cagtcccttt aacctcaaca tggttatgat
    25021 gtcctataag gataatggtg tgctcttttt gggcatagcg ctgagcttct ctatgaactt
    25081 ttgtaaccag aggacaggtc gcatccagta cctgtaattt acgattctct gctctttttc
    25141 gaacttccgg agaaactcca tgagcagaaa aaattgcatg tgaaccttca gggatttcat
    25201 caatattttc cacaaaaacg gcaccatcgt tccgaaggcg ctgaacaaca tgcttgttat
    25261 ggacaatttc atgctgaaca taaatcggag caccatacat ttccaaagct ttttcaacaa
    25321 tagtgatcgc acgatctact ccggcacaaa atccgcgagg actggccaat accaggttaa
    25381 gtggagcacc tgcgctgttt gtattcatag acaaagtaaa attatgaaga tggtttgcat
    25441 tttgctctaa ttagtttata agggtactga caacatttca atctttcagt aaaaactgag
    25501 aataccacca gaacgaaatc caagtcatga gtgaagaaga taaacgccgc aaggcgagct
    25561 ggaagcgctt gaaaggtacc accaattttt atcccactta tgttgacggg aaccttgaca
    25621 aggtcactcc tgctgctgcg acacacagca cacaatttca aaccgtcatt ccacgttacg
    25681 atctctccat gtctgaaacc cgcaacgcag ggactggtcc aacttttttg gaaacagctt
    25741 accaacctcc caccgagaat ccggatccag aaaaacaatc cccggacaca cctccggaat
    25801 catcggaacg aaagcttaaa cggccccgtc acaagaaaaa gaaaatccgt accgattttt
    25861 gggaataaaa caactcatcc aaacttctaa aaaagcaaat ataactgcca caaataaaat
    25921 tgctaccagt ttgatatact aaacttgtat catttatttg caggcaatag aagcagaaag
    25981 ttgagtaaaa aaaggaattc cttgcgccag cccggacagt atatttgttg atccccaagc
    26041 ataaactgca aaacagcttg acaaaaacac cattaatttg atctaatgtt gtgttaatca
    26101 cttttcaata agcctatgtt tgagaccctg tcagacaaat taagcggttc gttacgtaag
    26161 gtacgtggac ggggtcgttt gacggaagaa aatgtgagta aaactctaaa tgaggtcaaa
    26221 atggcccttt tggaggcgga tgttaatttt agagttgtta aggctttttt acgctcagtt
    26281 aagtccagag cggttggtga agaggtacag ggcagtctta ctccgggtca gcagttcatt
    26341 aaaattgtca acgaggaact gacagagatg atgggcggag ctaattctgg attaaccgaa
    26401 ccggaaactg ctccattggt cacaatgctg gttggcctcc agggatcagg taaaacctca
    26461 tccgcaggaa agttagcacg actttacaaa actgaaggga agcgccctta tttaattccc
    26521 gctgatatct accgaccggc tgctatccag caactgcaag tcttggcgga cacattggga
    26581 atttcatgct acccttcccg tacagatcaa aatccactgg atatagtcca gaatggactc
    26641 gaggctgcaa agcaggaaga agccgatgcc gtctttatcg acactgcagg acggttgcac
    26701 atagaccagg aattgatgga ggaattgagt cggatcaaag attccgtcat gcctcatgaa
    26761 attctttttg tcgctgatgc aatgacaggt caggaagcag tcagtgtggc aaaaggcttc
    26821 aatgatttgc ttgacattac gggtgtgatg ttgacgaaaa tggatggaga cgcccgcggt
    26881 ggcgcagcac tttctatccg ggccattacg caaaaaccga ttaagtttat tgcggtaggc
    26941 gaaaaactcg aaaatttgga agcattccat cctgaaagaa ttgcttccag aattttaggt
    27001 atgggcgata tgctctcact gatcgaaaag gttgaagact ctttcagtga acgtgaagcc
    27061 ctgcagatac agaaaaagct gaggcgcaac gaatttacgc ttgaggattt tagaaatcag
    27121 ttgcgttcga tgcgaaaaat gggatcaata aaagacgtaa ttggaatgct gcccggcatg
    27181 aatgcttctt ctgttaacga tgcaaatatt gacgacaaaa aactgattca tattgatgcc
    27241 atcatttcat cgatgactat taaagaaaga aataaccaca aactgatgaa tggctcgcgg
    27301 agaataagga tcgcaaaagg cagcggcaca accgtcagcg aaataaatag actgcttaag
    27361 caatttatcc aaatgcgtaa aatgatgcaa aagctcacaa aagtttccaa tccgggcaaa
    27421 gccatgcgca tgatgcagga tatgatgcct aattgaaggg tccgggtgct ggattcaggg
    27481 caccggcagg tcaaactgaa aactgaaaac tgaaaactta ttattatggt taaaatacgt
    27541 ctcgccaggg gtggtacaaa aaagaaacct tactaccgtg ttgtagttgc agatcaacgt
    27601 tgtaaacgcg acggtcgtta tcttgaacgc attggatttt acaatccaat ggtctcagaa
    27661 aaccgctttg gaattgatga agagcgagtc aagcactggc tttccgtggg tgcacaaccg
    27721 acggaacgag tcagcaaatt gatgaagcag gcaaaaatca aagtctgata agctttcggg
    27781 gttaatcgtt tttttcgata agtaccgcga tgagtgtttt taatgattat gatgagctga
    27841 cttgggttgc cacaattgtt ggtgctcacg ggatcaaagg agcagttaga gtaaaatact
    27901 ttacagatac tcctgattat tatctgaatg tagagctttt ctttttagaa tctgaaggac
    27961 agttacaaag cttaaaagtt ctgcagattc gaccgacaaa aagtggctgg atcgttctct
    28021 ttgaagaaat tgattcccgt actgctgctg aaaagcttaa aagctgccgc ttattattgc
    28081 cggatgatca gttaaggcct ttggaatcca gcgaagtctt cgtgcatcag cttatagggt
    28141 gcagtgtaga agataaaaac ggacgttttt tgggagaaat ttcagatgtt ctggaaaccg
    28201 gagcaaacaa cgtttatgag gtgcgtaacg gtaaaagcag tttcatgatt ccggatgttc
    28261 cgcatgtagt acttgaactg gatgttgagt cccggcggat gaaaattgac ccgttacctg
    28321 gattgattga agaaacagca ccggagagta atgttacgct ttgaagtttt aacattattt
    28381 ccggatattt ttacttcttt tctggaagaa agcctgatta aacgggcaat tgagaagcag
    28441 tacctgaatg taagtctggt caattttcgc aagcacggac acgggaaaca tctacaggta
    28501 gatgatacac cttacggagg cggaccggga atggtcttgc ggattgagcc gattgctcag
    28561 acacttaagg agcaaaaaga gtttcaccgc agcagtggcc gggaaactca cacaatttta
    28621 gtgactccgc agggacgacc tttttcacaa gaaaaagcaa gagagttaag taggaaaaaa
    28681 gcagtcttga cattgatctg cggccgttac gaaggattcg acgaacggtt acgcagtttg
    28741 gtggatgagg aaatttcagg tggagatttc atctgtatgg gaggtgaggt cattactatg
    28801 acaatgattg aaaccatcag tcgacttgta cccgacgttc ttggtaacca agaatcagct
    28861 gtacatgaat ctttcacgca gcccatgctg gaatttccgc aatacacacg acctgcaagt
    28921 tttgagggta tggaagttcc tgaagaatta aagtcaggaa accataaaat tatcgcccaa
    28981 tggcgtgaag aacaagcttt agaacggaca aaaaatcgcc gtccggactt gcttcaatgc
    29041 aaaactaaaa gttaaaatta aataatacag acaactaatt attcacctta gcaaacgaga
    29101 aaaaatgtct actgtaatgg aaaagattga gcaatcgcaa ttacgcagcg ggttgccgga
    29161 actaagagtc ggatccagcg tccgggttaa ttaccgaatt actgaaggaa acaaagaacg
    29221 catacaggca tttgagggtg tcatcattgg caagcactgc agtgcacgta acaacaaagc
    29281 cactttcact gtacgaaaag taacccaggg gtatggtgtt gaacgaattt ttccgctgca
    29341 ctctccccgg attgatagta ttgatgtcat taaagtcggg aaagtgcgcc aggcaaaact
    29401 ttattacctg agagcacgtc gtggaaaagc agcccggatc aaagaacgca tcacggcacg
    29461 gccatcagca taacttcagt ccaacacttg aacttgaaac agcggctcaa ggccgtggtt
    29521 ttcagcaagt tgctggtgta gatgaagcag gacgaggccc actggccgga ccagtgatgg
    29581 ttgcagcagt tattttagga aaagattgga atgccgagca tccgctgaac gattctaaaa
    29641 agctttcttc aacaaaacgg gaacagttat ttgacgttat ttgttctgag gctctggcat
    29701 ttaaaatcgt ctcaatttct gcagaagaaa ttgacaggct gaatattttg caggctacgc
    29761 ttcatggaat gttacgctgc ctgactgaaa tagaacctgc tccggattat gctcttgttg
    29821 acggaaacag gttcccgcaa actacaattc ggggtgaagc agtagttaag ggagatgctc
    29881 gttccaaaag cattgctgca gcttcaattt tggccaaagt gacacgggac cgagtaatgg
    29941 tcgaaaatgc aaaacgttat ccggaatggg gctttgaagg tcacaaagga tatccgacaa
    30001 aacaacatag ggcagccatt gagaaatatg gattgtcacc cttacataga cgatctttca
    30061 ggctcaaatc cctgtcaaag ctggacagat cgtctactgc tcaattgaat ttgctgtgac
    30121 aaaggcacga cagcagactg gagcagcagg agagcaaatt gcctgtaatt ttttgcaaga
    30181 gcaaggatat cgcattattg aacggaatca ccgttccaga cttggtgaac ttgatattat
    30241 tgcagcttac ggggagttcc ttatattttg tgaagtaaaa acccgcaggg gcagcagtgg
    30301 accgcatccg tctttatcgg taactgcgaa aaaaattgga aagttgcgcc aactgggtga
    30361 gctttatctc agccggaagc atctaacgta cctgcaaccg cgttttgacg tgattgctgt
    30421 acaactggcg gatggaaaac cgccgaacat tgatcatttt atcaacgctt tttaagaaaa
    30481 aaggaaaaca cttatggctc atcacaagtc agcgcttaag cgtgttcgac aaacaataaa
    30541 acgtactgca cagaagcgtt ctcagcgggc agatctgcgc acagtcatca aaaaattccg
    30601 cctgatactg gatggagaaa atatggatca ggcacgtgaa gcttattccg gagttcaaaa
    30661 aaatatcgat aaagctgtca ctaaaggaat cctgcacaaa cgtaccggcg cacgctacaa
    30721 gtcgcgcctt gcactgtccc tttcaaaaac tgtcgcaagc tgacatttct gtcgctttca
    30781 taattgcatt agatcgttct gatccgatag tcacagaaat aaccactttc taatttgctg
    30841 cctttcgtgc aaaaattaat gcagagtttt ctgctggttt atttaacgta tgtattttag
    30901 ttaagggtga tttatggact cacattttct aatgcggcgc attcacagtt tgacgggtgt
    30961 tgtcccggtg ggcctgtttt tggtgtatca tctctacctg caattgtatc tgcattcagg
    31021 ggcagaaaca tacaatactg cagtcaacag tttctatgac agtcccttgg cgatttggac
    31081 cctagtcata gttgtttata ttccgctctt ctttcatgcc ttccttggtg ttcgcctgat
    31141 ttttgaatcc accgtacaac cttcttacac atatttttca cacctgttgt attggctgca
    31201 gcgaatcagc ggcatcggcg tactcctttt cattctcgca catgtttgga acacccagtt
    31261 tgggccttgg atatccggga cctggggaac ccattttgag catctttcca gcggatttag
    31321 tgatcctgaa tcgggtataa taaccaagac ggtttacctg cttggtgtcc tgggagcagt
    31381 ctttcatttt gcaaacggct tcaacacctt ttgcatgacc tggggtatcg ccctcactcc
    31441 aaaatcccaa atacgggttc gtatttttag cattcttctt ttcttgattt tatcggcaag
    31501 tgccctgtat gcactgtctg caatttggta aacagatctt ttcagatgag caaacaacga
    31561 gtaattgtaa ttggaggagg tttagccgga ttggccgcaa ccatgaaaat ggcggaactg
    31621 ggcatgtcag ttttgctggt ttcgtttcta ccggtaaaac gctcgcattc agtttgtgcc
    31681 cagggaggca tcaacggggc agtcaatatc aaaggcgagg gagattctcc ggacattcat
    31741 ttttatgata ccgtgaaggg cggtgatttt ttagcacacc agccgctttg caaagatatg
    31801 tgccatcatg caccgtatat catcaacctg atggacaggc tcggagtaac tttcaaccga
    31861 actcctgaag gcaatcttga ttttcgcaga tttggaggaa cgctctatca ccgcactgca
    31921 tttgccggtg caactacagg ccagcagctt ttgtatgcac tcgacgaaca ggtacgccat
    31981 tacgaagtac agggcctggt cgaaaaaatg gagtggcatg aatatctcgg cgctgtacta
    32041 aacggtgatg gacgctgcgt tggtgcggtc atccacaatt tgcgttcagg cgaaattagc
    32101 acagaaaaag gcgatgccgt tattttagcc accggaggtc cgggactggt ttacggacgt
    32161 tccaccaatt ccatggtttg cacaggaact gcagttactt ctgcatattt acagggcgca
    32221 aaatatggga acggtgaatt tatccaaatt catccatcag ccattcccgg tcgtgataaa
    32281 ttacgtttga tgagtgaatc cgcacgcgga gaaggcggca gagtctgggt tccgcgaaaa
    32341 gccggggatt cacgtaagcc caaagaaatt ccggaaaaag aacgttttta ttttcttgaa
    32401 gagcgctatc cgctttttgg aaatttggtg ccgagagacg ttggagcgcg tgaaatttac
    32461 gatatttgcg tgaacgataa attaggcgtt cacggagaaa tgaaagtcta tctggatttg
    32521 actcatctca cgcgtgaatt tcttgatcat cgtctgggtg ggattcttga gatttacgaa
    32581 aaatttaccg gagtcgatcc tcgtgaagaa ccaatggaaa tttttccggc agtgcattat
    32641 tcaatgggcg gaatctggac tgattacacg agaactgagg aaggtttaat cgaccaccaa
    32701 tcgccacaaa atcaaatgac ttcaatcacc gggctttatg cggctggaga agcggattat
    32761 cagtatcacg gcggaaaccg cctgggagca aattctttgt tgagttgcat ttacaccggt
    32821 ttgatgatga gtccgggcgt gctcaattat gtcaaaaatg tcccggaagc ggctgatgat
    32881 gttcctgaaa aattgtttac ggaggcaact aaacaatggc aggaaaaata tgcggaaatt
    32941 aagaaaatgt ccggaaatga aaacccttat gcactgcaca gagaaatggc agaggaaatg
    33001 atttccaatg ttttgattgt ccgagaaaat tctaagttgc aggcaacgct cgaaaaaatt
    33061 gacgaatttg aagagcgttg gaagaacgta aactgtgttg atacaacaga ttggtcaaat
    33121 ccggttccaa gtttcatcaa ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33241 nnnctcattg cacctcgtcc caggaaatac gactgattca cataacatct gcacaatcgg
    33301 attttgaaaa agcgaaatcc gaaatccgaa aacttaaatc ataaatgata ttatgccgga
    33361 tactattact ttcaaaatta aacgtcagga ttcggaaagt tccggttcct actgggaaac
    33421 tttcgtcatt ccacatgtcc caaactccaa tgtaatttcc tgtttgatgg atattcaggc
    33481 aaatccggaa aactccaaag gagaaaaagt cgcaccggtg gtctgggaat gtaactgcct
    33541 tgaagaagtt tgcggttcat gcacaatggt gattaacaga aaggtgcgtc agtcctgcag
    33601 tgcattagtg gatcaacttg aaaaaccgat cactttggaa cccatgagca agttcccggt
    33661 aattcgtgat ttagcagtgg atcgttcccg gatgtttggt gccttgaaaa aagtacaggc
    33721 ctgggtcaca gcagacggct accacgatct tggacccgga atccgtgaac ctcaaaatca
    33781 tcaggaaatt gcctacaaac tttctgagtg catgacttgc ggatgctgcg tggaagcttg
    33841 tccgcaatac agtctcaaca acaacttcct tggtgctgcg gccatcagtc aatcacgcct
    33901 tttcaacatg catcctactg caaaaaacat agcggatgag cggctcgaca ctcttatgca
    33961 ggaaggtgga attgccgatt gcggcaatgc gcaaaactgt gttgaagcct gtccaaaatc
    34021 catccctctt actgaatcca ttgccgaaat aggacgccaa gtttcaggca aactttggag
    34081 caatattttt caggcatagt aaatgttttt tcctgaatat ttgataatgt attcatgtca
    34141 acaaatcctt catccgattc ccgcaaacgt ttcctgctga tccgcacgga tcgggttggc
    34201 gataccattc tgacttttcc tgcagttaca gctttacgca ataattaccc agaagcgttc
    34261 atctcatttt tggcacagcc ctataccgtt ccgttaattg aacagtatgc gggaatagac
    34321 ctgttgctga gttatgagcc tgagggacgt cacagaggct ggaagggagt attgaaactc
    34381 agtcatcagc tgcgggaact taattttgac gcggcaatac tgttttatcc ccgaccggaa
    34441 ttagcctttg ctctgctcaa ggcaaaaatt cctctccgga tcggtacagg ttaccgctgg
    34501 tattcattcc ttctgaacag gcgcatttac gaacaccgaa aagactgccg gaaacatgag
    34561 tctgaataca atctttccct gcttgaatca cttatgccgg acaaaataac ccagcctcat
    34621 tacgagttta agcaatggac accagaaccg tggtgggatg atttccagac agaactaaaa
    34681 tctaaggatt atgttatagt gcattccgga tgcggtgctt ctgctccaaa tttgaatgag
    34741 gagcaataca aactgataat acgactgtta ctggaaaaaa ctgactggac ggttttatta
    34801 acaggagttt ccggagaaga aaatgtggtg aatgcactgg ctgcggattt tccagaagag
    34861 agagtaaaga agactgttgg acgattttca cttccagaat tatttactgt gatccggaat
    34921 gcttcgttgt tgattgccag cagcaccgga cctttgcatc tggcaaatgc tgcaggaact
    34981 ccgctgcttg gatttttctg tcctgcaaaa ccacatacgc caactcgctg gggaccgtac
    35041 gatcagcaac aatgggtggt gacaccaaat ctggactggc ccgagatttg tgaattgaaa
    35101 aactgtccgc atggcggatg cctgaatcag ctttcagacg atgagattac tgaaattttg
    35161 tgtactcaaa gattaaaaac cattcacaaa tgaatcacaa acaaaataaa ggaatgacaa
    35221 aatcagtaac ctggcaaaat tttcagcagc attaccttga ccttccggag ttggggatgg
    35281 cacttgatac atctcgaatg aatgttccgg ataaactgcc gaatgaactg gaaatgcttt
    35341 tccgtaatgc ttttcaacaa atgcagaacc tggaaaaggg aggtatcgca aatcctgatg
    35401 aaaaccgcat ggtcggacat tactggctga gaaatcctga gcttgctcca aatgccgaga
    35461 ttcaaaaaga aatcagcagc acacttgaaa agattcaaaa ttttgtagca gaaattcacc
    35521 ggaacaaaat ccaaccgcaa aaagctgctt ctttccggaa tgtgctgctg ctcggaatcg
    35581 gaggttccgc gcttggtccg cagttcgtac taggtgcgct tggttctggg acggatcgaa
    35641 tgcaatttta ttttctagac aacactgatc cggacggaat cgatcaggtc atggcctcaa
    35701 tcggcaaagg attaggggaa actttgacgc tggttatttc caagtccgga ggaacaaaag
    35761 aaacccggaa cgcaatgctg gaggttcgag atgtctatga aaagaacgga ctggatttca
    35821 caaaacacgc tgttgcagtc acaggcgcaa acagtcaact tgatcaactt gcagcacagg
    35881 aaaactggct tgaccgcttt ccaatgtggg actgggttgg agggagaaca tcggtatttt
    35941 ctgcagttgg actgcttcct gcagctttac agggtttgaa tatctatgaa tttatagctg
    36001 gcgcaaaaac gatggatgaa attacccgta gtccggaagt cagcaataat cctgctgctc
    36061 tgctggcctg gatgtggcac catgcaggca gaggccaagg tgaaaaagcg atggtgctgc
    36121 tgccttacaa agatagattg ctgctcttca gccgttatct gcagcaactg gtaatggaat
    36181 caatcggtaa ggaaaa
//
LOCUS       DTVD01000026            3131 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_12258_length_3131_cov_315.938882, whole genome
            shotgun sequence.
ACCESSION   DTVD01000026 DTVD01000000
VERSION     DTVD01000026.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12258_length_3131_cov_315.938882"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..1347)
                     /locus_tag="EYO68_00960"
     CDS             complement(1..1347)
                     /locus_tag="EYO68_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC06405.1"
                     /translation="MPQKIQVKNIRHGNPGLSGWYAILPEPEPTHVLEEDLTADWLVI
                     GGGFAGLSAARRLLQLREKESIVLLDSIRIGEGSSGRNSGFMIDLPHDISSDSYAGAV
                     EQDIRQTMQNRYAIAFAAKTAEDYGFSQEVFNPCGKINGAASEKGDRHNREYVVHLNS
                     MGEESTWLDASDMKRITGSDYYLSGLLTPKTVTIQAAAYVRGFAQGLSRNVNIYENSP
                     VIAMKRADGIWKVNTPKGSVSAPKVILCVNGHLQSFGFLKRRLMHIFLYASMTRAMTT
                     EEIRRLGGEPSWGVAPANPFGSSVRKICGVGGHRILMRNKFHFNSTMEASDRDIQRTV
                     RNHDRSFQRRFPMLKNVEMEYRWGGRLCLSWNGVPVFGEIEDGLFAACCQNGVGVSKG
                     TLSGILAAEYASGFQNSYIEDYLNEEQPTRLPPEPLASIGATAYLNWQEWRAGLEK"
     gene            complement(1405..2310)
                     /locus_tag="EYO68_00965"
     CDS             complement(1405..2310)
                     /locus_tag="EYO68_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIC06406.1"
                     /translation="MKFEGIYTPIITPHTEDGSIHKEAFAEVAEFLIDAGVHCIVVGG
                     STGEYYAQTNEERIELMNFAREIINKRVLLMVGTGATRTEDCVMLAKAAKAAGADFLL
                     VNSPPYAVPTDEENAIHALQIDQAANLPIMLYNYPGRTGTHMGEEFLDRVGRSNNFCG
                     IKESSGDINRVHLLAHDYPNIGLCCGMDDQALEFFAWGARCWVCAGSNFLPKEHIALY
                     EACVLEGDYAKGRRIMSALLPLMRVLEQGGKFVQSVKYGCELTGLHPGPPRLPLQPLT
                     TDEKSKLERVIATLKKDISEILVSN"
     gene            2660..2932
                     /locus_tag="EYO68_00970"
     CDS             2660..2932
                     /locus_tag="EYO68_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06407.1"
                     /translation="MYPVVLGVRPEIVHATLGDRKVSTTLGVDAWISNGENMGPDLIV
                     FYEYHDTQLIGRFLKISEKPSSGEQIRVWMDCAGCSIFCQSTGQRL"
     gene            2886..>3131
                     /locus_tag="EYO68_00975"
     CDS             2886..>3131
                     /locus_tag="EYO68_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06408.1"
                     /translation="MQVVQSSANLPDSGYDRGKNPTRPLDNTDPGRAPVSHVAALFRG
                     LALRGGFHPGGHSDAGCRGGHRRDTLPELDIFALPTRL"
BASE COUNT          768 a          799 c          724 g          840 t
ORIGIN      
        1 ctacttttcc aatcctgcac gccattcctg ccaattgagg taagcagtag caccaataga
       61 ggccaatggt tccgggggca atctggtagg ttgttcttca ttgaggtaat cttcaatgta
      121 gctgttctgg aatccggatg cgtactccgc agctagaatt ccggagagcg tccctttgga
      181 aacaccgact ccgttttggc aacaggcggc aaacaatcca tcttcaattt cgccaaatac
      241 cggaactccg ttccaactca ggcacaagcg tccaccccag cggtactcca tttccacgtt
      301 tttaagcatc ggaaagcgtc gctgaaaact acggtcgtgg tttctcaccg tccgttgaat
      361 gtcgcggtcg gaggcttcca tggttgaatt gaagtggaac ttgtttcgca tcaggattcg
      421 atgtcctccc acaccgcaaa tctttctcac cgagctccca aatgggttag ccggcgcaac
      481 accccaactg ggttctcctc cgagcctgcg gatttcctct gtagtcatag cacgtgtcat
      541 tgaggcgtaa agaaaaatgt gcattagcct ccgcttgagg aatccaaaac tttgaaggtg
      601 tccgttgacg cagagaataa cttttggcgc agagacggaa cctttaggcg tgttgacttt
      661 ccagataccg tcagcacgtt tcatcgcaat gactggagaa ttttcgtaaa tattcacatt
      721 ccgggaaagg ccctgcgcaa atccccggac atacgcagcc gcttgaattg taaccgtttt
      781 tggggtaagc aaaccgctca ggtaatagtc acttcccgtg atccgtttca tatcggacgc
      841 atccagccag gttgattctt cccccatgga attaagatgc acgacatact cccggttatg
      901 ccgatctcct ttttcgctgg ccgccccgtt gatttttccg cagggattga aaacttcctg
      961 tgaaaaaccg tagtcctctg cggttttggc ggcaaaggca attgcatagc ggttttgcat
     1021 ggtttggcga atgtcctgtt ccacagcacc ggcatagctg tcgctactga tgtcgtgcgg
     1081 taaatcaatc atgaaaccgg aattgcgtcc ggaactgccc tcacctatgc ggatggaatc
     1141 cagcaaaact atactctctt tttcccggag ttgtaacagc cgtcttgcag cagacaaccc
     1201 agcgaacccc ccaccaatta ccagccagtc ggcagtgagg tcttcttcta aaacatgtgt
     1261 tggttccggt tccgggagaa tcgcatacca accggaaaga ccaggatttc cgtggcggat
     1321 gtttttgacc tgaatcttct gcggcattag tattctgtct gaaattttta aagggaaccc
     1381 ggtacaacca agtttagatt taactcaatt gctgacgaga atttcggaaa tatctttttt
     1441 taatgtagcg atcacccgtt ccagtttgct tttttcgtcc gtagtcagcg gctgaagtgg
     1501 caggcgggga ggtccgggat ggaggcccgt cagttcacag ccgtatttga cgctttgaac
     1561 aaacttccct ccctgctcca gaacacgcat tagtggaagc aacgccgaca tgattcgacg
     1621 cccttttgcg taatcgcctt caagcacaca ggcttcataa agtgcgatgt gctctttcgg
     1681 caaaaagttt gaaccggcgc aaacccagca tctggctccc caggcaaaga actcaagcgc
     1741 ctgatcgtcc atgccgcagc acagcccgat gttgggatag tcgtgcgcga gcaggtgaac
     1801 gcggttaata tctccggaac tttccttgat gccgcagaaa ttgttagagc gtcccacacg
     1861 atccagaaac tcctcaccca tatgagtccc ggtacggccg ggatagttgt aaagcatgat
     1921 aggcaggttt gcagcttgat caatttgaag tgcgtgtatt gcgttttctt catcagtcgg
     1981 caccgcatac ggaggagaat tgaccagcag gaagtccgct cctgctgctt ttgccgcttt
     2041 tgctaacatc acgcaatctt ctgtacgtgt agctcctgtt ccaaccatca gcaaaacacg
     2101 cttattgatg atttcacggg cgaagttcat cagttcaatt cgttcttcgt tggtctgtgc
     2161 gtaatactct ccagttgagc cgcctactac aatgcaatga actccggcgt caatcaggaa
     2221 ctctgctact tcagcaaagg cctccttgtg aatggatccg tcttctgtgt gcggtgtgat
     2281 gatcggtgtg taaattcctt caaatttcat tggtttcctt aaatttagtg tatatttttt
     2341 atgccctgat cttaaataat atactgttca cgtgggcgat gtggttcaga agtttttcat
     2401 tgttgtctca gtccagtttc gattcgtcta ttgtacaaaa cacttccaac attaaatttt
     2461 attatatctc ctcaaaaaaa ggatacaaac cacaaaaaat attttaccat tcaatactta
     2521 tcaaatgaag aaaaatcttt ttcctaaaaa tgaatttaat ctctggaaaa atagctcaat
     2581 ccgagggtaa agtgtcggta caatcaaatg gtctgcttta cccgagaagc cattacccat
     2641 ttcgaaaccg tccagtaaga tgtacccagt ggttttagga gtgcgtcctg aaatcgttca
     2701 tgctactcta ggagatcgaa aagtaagcac cacgcttggc gtggacgctt ggatctccaa
     2761 cggggaaaac atggggcccg acttgattgt cttctacgaa tatcatgaca cacaactcat
     2821 cggacggttc ttgaaaattt ctgagaaacc cagcagcgga gaacaaatcc gtgtctggat
     2881 ggattgtgca ggttgttcaa tcttctgcca atctaccgga cagcggttat gaccgaggca
     2941 aaaacccaac acgtcccctg gacaacactg acccaggaag ggcacctgtc tcgcatgttg
     3001 ctgctttgtt tcggggtttg gctctacgcg gcggattcca ccctggtggc cacagtgatg
     3061 ccggttgccg tggaggacat cggcgggata ccctacctga gttggacata ttcgctctac
     3121 caactcggct c
//
LOCUS       DTVD01000027            3119 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_12341_length_3119_cov_202.105418, whole genome
            shotgun sequence.
ACCESSION   DTVD01000027 DTVD01000000
VERSION     DTVD01000027.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12341_length_3119_cov_202.105418"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            76..534
                     /locus_tag="EYO68_00980"
     CDS             76..534
                     /locus_tag="EYO68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC06409.1"
                     /translation="MHSLKINGILHQVDVEDEMPLLWVLRDELGMTGTKFGCGIGQCG
                     ACTVHVNGEAVRSCSFPVFAAQDKEIITIEGLGNNHPVQQAWIEEQVPQCGYCQTGQI
                     MATSALLSQNLEPSETEIENKITNLCRCGTYVRVRKAIRRSAELIQQQVS"
     gene            536..2653
                     /locus_tag="EYO68_00985"
     CDS             536..2653
                     /locus_tag="EYO68_00985"
                     /inference="COORDINATES: protein motif:HMM:PF02738.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC06410.1"
                     /translation="MGKYSLSRRVFLKKSALTFTGLIIGFTYEPESSLAVKVENAEEL
                     GIWIRIAPDGSTTLIVPSSEMGQGVNTSLSMILAEELEADWQSVKTETAPVNSKYINP
                     ESNSGQMTAGSSSVKGFWNPLRKAGAAVRLMLQKAAAQKWEIPIEECVAKSGYIYRKN
                     SSQKLSYGELAEAAGKIDIPSDPPLKNSKDYNLVGKSIKRIDIPSKTNGSAQFGIDVR
                     LPEMMYATIRQSPVFGGEVLSYDEDAAKSIRGVKKVTLIPNGIAVIADNTWRAKRGME
                     VLNLKFHGGKTIGLESQQVQKELLKALDDEGKTKIEANQVLDLEYEVPFLAHAPLEPM
                     NCTANVTDNFCEVWVPTQSQGGCMDAAKEITELDEEQIQIHTTYLGGGFGRRGETDFV
                     KQSLILAKALGKPIKVTWMREEDMQHDFYRPASMSRFQIGLNKDGFPISWNNQLASPS
                     ILKRFFAPMAWFNIDPLSTEGADEIPYAIEDFNFDYSVVDSGVPVGFWRSVGSSFNAF
                     FTESAIDEAAHLAQQDQFDYRMKLLSGKPRFQKVLEKVMRESKWGRILPKGHGLGIAI
                     HKSFGSIVGAVIEVSTDVNGMLNLNKAWIAIDCGKVINPNTVRAQMEGGFTFGLSATL
                     GEEITLKDGRVEQSNFHDYSILRLKGSPEISVEIIESGNEIGGVGEVAVPLAAPALTN
                     AIFSSSGRRIRSLPLSKHGIKIT"
BASE COUNT         1022 a          526 c          703 g          868 t
ORIGIN      
        1 acaggagatg atatcattga ctttttttaa atagttattt agattttaaa aacacttttt
       61 aaattaaaat aagttatgca ttcattaaaa ataaatggaa ttcttcatca agttgatgtc
      121 gaagatgaaa tgccgttact ctgggttttg agagatgagc ttggaatgac tggaacaaaa
      181 tttgggtgtg gaatcggtca gtgcggtgct tgtaccgtac acgtgaatgg agaggcagtt
      241 cgttcttgca gtttcccagt atttgcggcg caggataaag aaattataac tattgaagga
      301 cttggcaaca atcaccctgt tcaacaagct tggattgaag aacaagttcc tcaatgtggt
      361 tactgtcaga ctgggcaaat catggcaacc tcggctttat tatcccaaaa cttagaacca
      421 agcgaaacag aaatagaaaa taaaattaca aatttatgcc gttgtggaac ttatgttcgg
      481 gtaagaaagg caattcgccg gtcggcagaa ctcattcagc aacaggtaag ctaaaatggg
      541 aaaatattct ctttcacgca gggttttttt gaaaaaatct gcactgactt ttacaggttt
      601 aattatcggc ttcacatatg agccagaatc cagtttggcg gtaaaagttg aaaatgcgga
      661 agaactcggt atttggattc gaattgcacc agatggatca acgacattga ttgttcctag
      721 ttctgaaatg gggcaaggag tgaatacttc actttccatg atattggctg aagaactgga
      781 agcagactgg caaagtgtta aaacggaaac tgcaccagta aattcgaaat acatcaaccc
      841 tgaaagtaat tcaggtcaaa tgacggctgg aagttcaagc gttaagggct tttggaaccc
      901 gttaagaaaa gcaggtgcag cagttcgact aatgcttcaa aaggctgcag ctcaaaaatg
      961 ggaaatacct atagaggaat gtgtagctaa gtcaggatac atatatcgta agaatagcag
     1021 ccaaaaatta agctatggtg agcttgctga agcagcagga aaaatagaca ttccttctga
     1081 tcctccttta aaaaattcta aagactacaa tttagttggc aaatcaataa aaagaattga
     1141 tattccaagc aaaacaaatg gatctgccca attcgggatt gatgtaaggt tgccagaaat
     1201 gatgtatgca accatccgac aatcccctgt ttttggaggt gaagtcctat catatgacga
     1261 ggatgctgca aaaagcatta ggggggtaaa aaaagtgact ctcataccta atgggattgc
     1321 agttatagca gataatacct ggcgtgcaaa aagaggaatg gaagtcttga atcttaaatt
     1381 tcatggaggg aaaactatag ggcttgaaag tcagcaagtt cagaaggaat tgcttaaggc
     1441 tctggatgac gaagggaaaa ctaaaattga agcaaatcaa gttttagatc tagaatacga
     1501 agtacctttt ttagctcatg ctccccttga accaatgaac tgcacggcaa atgttacaga
     1561 taatttttgc gaagtctggg ttccaactca atctcaagga gggtgcatgg atgccgcaaa
     1621 agaaattaca gaattagatg aggaacagat tcaaattcac acaacttatc ttggtggtgg
     1681 gtttgggaga cgaggagaaa ctgattttgt aaaacaatca ttgatacttg ctaaagcatt
     1741 agggaaaccg attaaagtaa cctggatgcg tgaggaagat atgcagcatg atttttatcg
     1801 tccagccagt atgagtcgtt tccagatagg attaaataaa gatggattcc ctatatcttg
     1861 gaataatcag ttggcctcac cttcaattct taaacgcttt tttgcaccaa tggcctggtt
     1921 taatatagat ccattgtcaa ctgaaggcgc ggatgaaata ccttatgcaa tagaggattt
     1981 taattttgat tattctgtag ttgattcagg tgttcctgtt ggattttggc gttcagtagg
     2041 tagttcattc aacgcttttt ttacagaaag tgcaatagat gaagccgccc atctggctca
     2101 acaggatcag tttgattatc gcatgaaact tttgtcagga aaacctcgtt ttcagaaagt
     2161 cttagaaaaa gtgatgagag aatctaaatg gggaagaata cttccaaagg gacacggatt
     2221 gggaatcgca attcataaat cgttcggaag cattgtcgga gcagtgattg aagtttccac
     2281 tgatgttaat ggtatgctaa atttgaacaa agcctggatc gctattgact gtggaaaagt
     2341 aataaatcct aataccgtca gagctcagat ggaaggaggc tttacttttg gtttgtcagc
     2401 aactttgggt gaagagatca cgttgaaaga cggccgggtt gagcaaagta attttcatga
     2461 ttattcaatt ctaagactaa agggttcacc agaaatttcc gttgaaataa ttgaaagtgg
     2521 taacgaaata ggtggtgttg gtgaagtagc cgtaccactt gcagcaccag ctctaaccaa
     2581 tgcaattttt tcttcatctg gaagacgcat ccgatctttg cccttgagta aacatggaat
     2641 aaaaatcacc tgaatacaga ctttagtgag ctccatagta gtttcaattt cggtacttat
     2701 accatcaaat ggaatgtttt agaagaatag agtgatttaa cttggagtgt gtctgattgt
     2761 taagaattag tgggggttga tcaaggagcc aaatatgaaa cactttcata agccttaatg
     2821 cacaagtgca tcaaaaacaa tagacacgct ccaagggggg atttttctcc tgcaaaaagc
     2881 cccagccaaa atttttatag aaggaaaatc aagaagcctt gaacaatttt cttgtgccac
     2941 tcctgtgcca gtagctcaca aaaaaggagg aaaaagaagg ataagagggg aacggtttaa
     3001 acatttaact tgctaatatc attgacctat cttatcatgg attgacgtgg attacccggc
     3061 cccaatgact gttaatcatc gggtcactgg ttcgagtccg gcttggggag ccattattg
//
LOCUS       DTVD01000028            3110 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_12394_length_3110_cov_386.641899, whole genome
            shotgun sequence.
ACCESSION   DTVD01000028 DTVD01000000
VERSION     DTVD01000028.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_3110_cov_386.641899"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            413..1171
                     /locus_tag="EYO68_00990"
     CDS             413..1171
                     /locus_tag="EYO68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIC06411.1"
                     /translation="MKQRLDRLLVEQKLAPSREKAQALVMAGKVRVDGQPAGKSGRVI
                     DVDSHLEVLGDDQPFVSRGGVKLQAALEYFNINPSGLVALDIGSSTGGFSHCLILGGA
                     EKVFAVDVGYGQLAWELQQDSRVVSLERTNFRHLEFERLGETVDLVVIDVSFISLRLI
                     FPKALEFLKPGGTLLALVKPQFEAGAAEVGKRGKVSDTDVHERVLEALKDKAQELGFL
                     VSGQTKSAIVGKKSGNVEFFLWLKKSPKFDSNAS"
     gene            1168..2040
                     /locus_tag="EYO68_00995"
     CDS             1168..2040
                     /locus_tag="EYO68_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIC06412.1"
                     /translation="MNRDHSPLNLSDETLLHVSLEQIIPNPKQPRKHFSQKTLLELAQ
                     SIRTQGILQPLVVRKHQNLTNCYELVAGERRWRALKQLEVNEVPVVLREVGDDEMLEV
                     SLLENIQRENLTIIEEAQAYHDLLKIHDYTQEELAQRIGKDRSTITNLMRLLKLPSAL
                     KNDLETGRITSGHARSILALPGEAFQLEMRQRLLRHSWSVRDTERQVRLKLNSLPAGL
                     ETQGNKKAVSGGPVTEGSVADHDAQLQNLEEKLQHQLQTRVRIKYHEGKGQIQIDYFS
                     LEEFDRLYDLLISS"
     gene            2289..2972
                     /locus_tag="EYO68_01000"
     CDS             2289..2972
                     /locus_tag="EYO68_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIC06413.1"
                     /translation="MRFGSTTTLPRGVTPAASVTTTDRLVFIKKVYSLLAMSMGTAAI
                     GAYLGSGPLLLLVAPNMMLFFILQIALIFFASFAARKPGLNMVALFSFTTVSGLTLGP
                     LLYQVGPSIAAEAFALTAITFAGLSMYVVYSKKDFSFMSGFLMTGLIVLVVGGLLNMF
                     FIQSGMMHFVMSGASVLLFSGFILYDTSNILRYYGTDEYVSATLALYLDVLNLFIALL
                     SILGIMSDE"
BASE COUNT          888 a          606 c          710 g          906 t
ORIGIN      
        1 aatttggctg caagacttga aggcgccaat aaattttata aaacctactc catgatttcc
       61 ggcagtacct atgaactgac caaagaggac gtcgacagtc gtcaactgga tattatccgg
      121 gttgtcggta aaaaagagcc ggttcctgtt cacgaactgc tggcccgtaa aaacgaaaca
      181 agcagtgaaa tgagcggtgt ggttgagcag tacctgaaag gattgaagtt atatcaggat
      241 aaaaattttg ctgatgcagt taaggaattt gaaaaagtct taaccataga tccggaagat
      301 ggtccatccc tgacttatgt taagcgctgc ggcatgttca ttgagacacc tccggaaaaa
      361 gactgggacg gtgtgtttac cttcactgaa aaggggtgag taaaaactat cgatgaaaca
      421 acgtcttgac cgactgcttg ttgaacaaaa acttgcaccc tcccgtgaaa aagcacaggc
      481 gcttgttatg gcggggaaag ttagagttga tggacagccg gcaggtaagt caggacgtgt
      541 aatcgatgta gattcgcatt tggaagtact gggtgatgac cagccttttg tgagtcgggg
      601 aggtgtgaaa ctccaggcag cacttgagta ttttaatatc aatccttcag gtttggttgc
      661 tctggacatt gggtcttcga ctggcggatt ttcacattgt ctgattctag gcggtgcaga
      721 aaaagtgttt gcggttgatg tcggctatgg ccagcttgcc tgggagttgc agcaggattc
      781 gcgtgttgtg tctctggaac gtacaaattt tcgtcatctc gaatttgaac gtcttggtga
      841 aactgtagat ttagtggtga tcgacgtatc tttcatttca ctgcgtttga tatttccgaa
      901 agcgctggaa tttctaaaac ccggaggaac cctgttggct ttggtcaaac ctcaatttga
      961 agctggagca gctgaagttg gtaaacgtgg gaaagtgagc gatactgacg tacacgagag
     1021 agttttggaa gctctaaaag ataaagccca agaactgggg tttctagtgt caggtcagac
     1081 aaaatcagca attgtcggta aaaaaagtgg taacgtagag ttttttctct ggctgaaaaa
     1141 atctccaaaa tttgactcta atgcctcatg aatagagacc actcgcctct caacctttca
     1201 gatgaaactc tgcttcatgt ttctctggaa caaattatcc caaatccaaa acagcctcgc
     1261 aaacattttt ctcaaaaaac actactggag ctggctcagt caattcggac ccagggaatt
     1321 cttcagcctt tggttgttcg aaagcaccag aatcttacga attgttatga gttagttgcc
     1381 ggagaacgtc gctggcgtgc gttaaaacaa cttgaagtaa atgaagttcc ggttgtgttg
     1441 cgagaagttg gcgatgatga aatgctggaa gtgtctctct tggaaaatat tcagcgagaa
     1501 aatctgacaa ttatagaaga ggcacaagct tatcatgacc tcctgaaaat tcacgattac
     1561 actcaggaag agttggctca aagaatcggc aaggaccgtt ccacaatcac aaatctgatg
     1621 cgcctactca aacttccatc tgcattaaaa aatgatctgg aaacagggcg catcacttct
     1681 ggtcatgcca ggtcaatttt ggcacttccc ggtgaggctt ttcaattgga aatgcgtcaa
     1741 cgtcttttgc ggcattcctg gtcggtacgt gatacagaac gacaagtccg gctaaagctc
     1801 aattcactcc ctgcgggtct ggaaacacag ggaaataaaa aagccgtaag tggaggtccc
     1861 gtaactgaag gttcagtagc agatcatgat gcgcagttgc aaaatctgga agaaaaacta
     1921 caacaccaac tgcaaactcg tgtgagaata aaataccatg agggcaaagg tcagatccag
     1981 attgattatt tttctttgga agaatttgac cggctgtatg atttactaat ttcttcctga
     2041 ttatataaag aaaatgcagt ctcatattgt taagtaaaaa atgagaataa ttgagaaaaa
     2101 tgaaaagagt gttcaaagtg attttggttt agatcaaatg ttttggccca atggcaagtg
     2161 gtgcaaatta atgaaaattt cttttacttt tttaacattt tcctgtagaa taagttgaca
     2221 gttagaattg gtttaaccaa tttattgttt tgaatgaatt ttactctaaa aaaaattagg
     2281 aggttattat gagatttgga tcgacaacta ctctcccccg aggcgttaca ccagctgctt
     2341 ctgtaacaac aacagaccga ttggttttta taaaaaaagt ttacagcctg cttgccatgt
     2401 ccatgggaac tgcagctatt ggagcatatt tgggatcagg gccgctgctg cttttagtgg
     2461 caccaaacat gatgctcttt ttcatcctgc aaatcgcact gatcttcttt gcttcctttg
     2521 ctgcacgtaa acctggcctg aacatggtgg cacttttcag cttcaccact gtttcaggat
     2581 taactcttgg tcccttattg tatcaagtag gaccgtctat tgctgcagaa gcatttgctt
     2641 tgactgctat cacgtttgca ggattatcca tgtacgttgt ttattcaaaa aaggatttta
     2701 gtttcatgtc tggattttta atgaccggac ttatagttct tgtcgttgga ggtcttttga
     2761 acatgttctt tattcaaagc ggaatgatgc acttcgtgat gtctggcgca agtgtattac
     2821 tgtttagcgg tttcattctc tatgacactt ctaacatatt gcgctactac ggtacagatg
     2881 aatacgtctc cgccacactg gcattgtatc ttgatgtgct caacctgttc attgcccttt
     2941 tgagtattct tggcataatg agtgatgaat agaagataaa ggaaaagagg ccgggaaact
     3001 ggaggacgta accgttcaga attcccgtgc ctcaattctg ccatcttgat cttcaaatct
     3061 cctagttcct taattgcaaa ttcgctgcaa tctgaacatc agttatttag
//
LOCUS       DTVD01000029           35800 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_126_length_35800_cov_400.394461, whole genome
            shotgun sequence.
ACCESSION   DTVD01000029 DTVD01000000
VERSION     DTVD01000029.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 35800)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35800)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35800
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_35800_cov_400.394461"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="EYO68_01005"
     CDS             <1..1768
                     /locus_tag="EYO68_01005"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIC06414.1"
                     /translation="WSKVMSKSKFSEKIGTEPSGWFEGARLNFAENLLAQGNDENEAL
                     CFIGENGSTSSLSYEELRNAVAQCASGLKSLGVSKNDRVAAVMPNCPEAVIGMLATAS
                     LGAIWSSCSPDFGINGINDRLGQISPKILITVNAYHYNGKEHDCLLKIREIVSRIPSI
                     ELVFIVPFTETTIKFQGNEQAWGKLLNSSAGTVNFAQLPFDHPLYILYSSGTTGLPKS
                     IVHGAGGTLLKHLTEHRYHADVHPRDRMFFFTTCGWMMWNWLVSGLASGATLILYDGA
                     PGFPDIGRMWRLIDEQQITHFGTSPKFLGAVSKTGYSPKQNHQLESLRAILSTGSPLP
                     ADLFSWVYAEVAYVPLMSVAGGTDIAGCFMGGNPNLPVYSEELQCKSLGMNTLAYNSD
                     GESVVCEKGELVCADPFPSMPIYFWNDADGKKYHKAYFEKFPGVWTHGDFIEITERGG
                     IIVYGRSDTVLNPGGVRIGTAEIYRPVEEMQEVEDSLVVAQPWESDVRIVLFVVLRDG
                     IRLDENLVAKIKTVIRSDTTPRHVPARILAIPDVPRTISGKKVEKAALQTIKGEYIEN
                     TSALANPGSLEHFSKLSEELRK"
     gene            complement(1785..3203)
                     /locus_tag="EYO68_01010"
     CDS             complement(1785..3203)
                     /locus_tag="EYO68_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06415.1"
                     /translation="MHRNATFREYSEHFDSLLPDQDSLLIQLCEHLFNSGFVFVDASE
                     NELEAGGWVIRDFFDQEAEVLRTLVPRERYVGFIDYVKQFNWPAEILQKKLSQRIQKS
                     FDQLAKGKHHHFNIRYNISYGLREQEKHPLYDFLLHIETYYSLVLGGETLEQEDQEEE
                     PDFSKTNFKIEDLKQLRSQVRERLEGRELVKGFLKHIAKKRGVSVESLNPVIKQERKN
                     SPIKFLRPSQLNPTAEKRTAPDVAEILIAEEKNDIVDKLDISSTLTEDQNELSEFPLE
                     PDLETDIAETAEVLIESKKAEEGIFEEETVASQTIEDAAVEVEEEDELKEKNNYEPET
                     EADSSTVTTADLLLNFEEDEEDEEIEEDDSGLILVDSDETDVVFGGEKISKAALEKFI
                     RQYPDSTLRFLLRRSLDGRPLSAEIEAIYYSWEKRGLSRGRLKKYLLKLMEWPEVPDL
                     PILDLLQSLKDRIYEDSQKVET"
     gene            3514..5451
                     /locus_tag="EYO68_01015"
     CDS             3514..5451
                     /locus_tag="EYO68_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC06416.1"
                     /translation="MISIADLAMNYGKKTLFEKSSVTFNPGKSYGLVGANGSGKSTLL
                     RLIAGEEKPNSGTISVPRDLKLGVLRQDHFQYENIKVIDVVLLGKPALCEALREKEKI
                     LESENLDSQSGHRLGQLEEMIAEADGYVAEPFAAELLSGLGIGTGFHTGPMSALSGGF
                     KLRVLLAQLLFQQPDVLLLDEPTNHLDIVSIRWLENYLCTQFEGTLIFISHDRNFLNV
                     VARYIIDIDYEELRLYTGNYEQFLEAKTLAETQKRKEIENYERKTAELQAFVDRFRAK
                     ATKARQAQSRVKQLDKMEVPEVKRSSRISPDLSFEQFRPSGKTVLTVKDVSKKFGNTE
                     VLKNVSFEIQRGEKVAIIGPNGIGKSTLLKIILAQLSADEGTFEWGYETHTSYFAQDH
                     HEHLRGKISAYDWLYATAPHEPIGAIRGLLGRVLLSGDEALKQVTALSGGESARLLFA
                     RIMLEKRNILILDEPTNHMDLEGVAALGDALKAFEGTVLVVSHYRHFVSKVATHILEL
                     TPHGVRDFSGSYQEYLEKFGDDYLSRDQPLSTTHRETKATPSTQELSYAERKKLQSEI
                     SKLKKQSTRFETLIEEGEQKISAIEAKFGVENFYQQASPEEIQNLQTEKEKLSAQLST
                     HLSQWETSSHKLEVAQARFGG"
     gene            5644..9144
                     /locus_tag="EYO68_01020"
     CDS             5644..9144
                     /locus_tag="EYO68_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC06417.1"
                     /translation="MRKSNSIFKFGFLGLILTLLQILPLNNTSFSQTTPEKAAELWNL
                     RQSEIQQLYQEGNLEGALNSAQESVSLAEVAFGSGALKTISSMLLQAQIHGELDQLEE
                     ANQIYQVALETSVAAFGESDATTLSVLDNYGGFLKSIDPEMSEPVLQEALRLTTEDDP
                     QRAARLRALGLNLQELGRIGEAEGMLSEALKAYKKVLGENSPETLSSMADLAQLYLTQ
                     GKLKPAQELFETALPLMLEVMNKTSFLILASKENLAEIYRQQGKFSLAEKLFLESMQG
                     AVAEYGEEDSLVIQARSHLAQLYEDTGKLKKALLLHQQVYEVDLALLGEAHPNTAGDL
                     NNLASVRRRLGDYQKAEKLFRQSLKVMIAALGEETPQTVSVMNNLALLLENQGLYDEA
                     EPLFKKAFAISGKIQGEKHPTSLALLNNLAFLNESQGDFIRSELNYKKVIQLNTEVFG
                     ENHTNTIANQNNLAYLYLRDERYNEAQPIFTKVHSQWSKLLGAEHQNTLKALNNLGRV
                     QKSLGNLEAAEKHLMEALAGRTKVFGKRHADTLRSMNDMGELFVVQEKLDEALKMFQE
                     TLKLEEEVLGENHPYTFETLNNLADLLKIMGKTEEAYKLFMTGFERRNAFLDRVLWVA
                     GDNTRQSYISLHRPEQNALIRMLLSLDDERTARDLFEVSLRRKGILLKITSETQQVLM
                     LSGSPELQAITEDLTQTRKTLAGLTLSGPTDENRKEFPKIIFDLEEKVNMLQLKLGEQ
                     SVRFRQSTKQVTVDEVVEGMGENAMVDFMSFRGEDDSLRMLAVVVNAGEFSFVEYEDL
                     ELISAIVMELREIVQDEGAEDEDIKSVAYDLWEILWQPLTESLGETASIFISPDSLLN
                     VLPFDVLTDSDDSYLLENFDLRIISSARDLALDPLLAAKGDLMIIAGPDYDSDKILKT
                     PAAREVTHKRSRSVARGARMGSGLRGLNFDPLPGAEKEGEVIKEVSDTKDRNTTIFSQ
                     RVAEEKLLREMSAPPEVLHIATHGFFLKEDEKLKKRLQGLSRGSSSVPPPADNPLLRA
                     GLAFAGLNANASMLGEIDTDNDGVLTAMEVLSLDLAGTQLVVLSACETGLGEIHEGEG
                     VYGLRRSFQEAGVKNVINSFWEVSDAGTQLLMTKFYDKFLAGMPAREAMREARLEMLD
                     DVQWSAPFYWSAFVMVGRNT"
     gene            9149..9997
                     /locus_tag="EYO68_01025"
     CDS             9149..9997
                     /locus_tag="EYO68_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIC06418.1"
                     /translation="MEQLVIHGGAGSLEGKTTEAQKMHDSLCKIWEETFEVLQKRSAE
                     DAVRHAVRMLEDDPVYNAGTGSKLQADGQIRMSAALMDGTNNRFSGVINVQNIRNPIE
                     AAALLSGEKHSILSSAQATEFCRTKGIPEYNPETEFRLHEYKSNCAGIHGTVGAVALD
                     KDGHIFAGTSTGGIGCEIPGRVSDSPTVAGTYASTKAGVSCTGIGEQITQQAAAAKVV
                     TRVGDGMLLNEAVTRTMHESDDFNYLYGLISLDSEGHVEVGKTAALSAVFYAFHDGEK
                     IRTFFE"
     gene            complement(10013..10555)
                     /locus_tag="EYO68_01030"
     CDS             complement(10013..10555)
                     /locus_tag="EYO68_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06419.1"
                     /translation="MRFFILYLLLILTLSGGCYPESLNYRPGWGTQWIDGTLLEENGN
                     DLKSKGFVVVLEYYSQFIQFKNESPLYAPKARLVFPEEDGRFRIHFDLEASAIELVFV
                     ASGYTMERFRFRRQIGIGKLHFDAKLSQSEMWRNQFLLQTGPFLDNFILEQRYQMPDS
                     QQLFIGEWLDNERSKYSAGN"
     gene            10827..13544
                     /gene="secA"
                     /locus_tag="EYO68_01035"
     CDS             10827..13544
                     /gene="secA"
                     /locus_tag="EYO68_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIC06420.1"
                     /translation="MWKKVKNIFGDENAKTLNGYRKVADKINALEPEIQALADEEFPK
                     KTTAFQTRLEKGVALDELIPEAFALVREASLRVIGERHYDVQLQGGLALHHGNIAEMA
                     TGEGKTLSSTCPVYLNALTGKGVHIITPNDYLAKRDSKWMGAIFEFLGLSVGLIQHGI
                     YDEERRAAYEADITYGTNNEFGFDYLRDNMKFSLEDYVQREFNFAVVDEVDSILIDEA
                     RTPLIISGPTEDNIDKYHQINKFVYGLSREIRKEEVGKLPQDQMHNIAENLEELEDHD
                     IVRDGDYALDERARSISLTDQGSVKIEGRLRDQLVKDTSLFDYENMEILHHVNQALKA
                     HYLFKNDVDYVVQEGQVIIVDEFTGRLMPGRRFSDGLHQALEAKEGVNVERENQTLAT
                     ITFQNYFRLYDKLSGMTGTAETEKNEFKKIYNLGVVVVPTNKPLIRKDLSDVVFKTVD
                     AKYRATVDRIKVLNEKGQPVLVGTVSIEVSEAISKLLKKAGIEHEVLNAKHHEREAEI
                     ISNAGQFGAVTIATNMAGRGTDIKPSPETLEVGGVFVLGTGRHDSRRIDNQFRGRSGR
                     QGDPGASQFLLSLEDDLLRIFGGERISNLMDKLNIEEDEPIEHVFITKAIGNAQKKVE
                     GYHFDIRKHVLEYDDVMEKQRSIIYGRRREILGEEVQNLILEMCDGVVDQLMEQHCQD
                     KYVDQWDVQGFNRAFEGVFSKVPNEKWFEDELNPDEYSEKFYGWIEDLYKEKIEFFRN
                     VAELNFEPDVSTEDRKEILGQMILDLERQILLKVNDNLWKDHLLSMDMLREGIGLVGY
                     AQKKPLDEYRKQAFEMFSDLMNRIDNEAISTFFKLTIARPIAEPEPAPLLKDVEFIHG
                     EVEIPDGTETKKKKPQPVRAQSTVGRNEPCPCGSGKKYKRCCGLAKKIA"
     gene            13641..16316
                     /locus_tag="EYO68_01040"
     CDS             13641..16316
                     /locus_tag="EYO68_01040"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIC06421.1"
                     /translation="MTSLAKAYDPQAFESKIAEQWENSDAFRADAHSNKPAFTISMPP
                     PNATGQLHVGHAVMLALEDIFIRWHRMQGHEALWLPGTDHAAIATENVVLQQIKKEEG
                     IADPREVLGREEILRRIAEYVESSQGTIRNQVKALGSSCDWSRERYTMDPQLNRCVNE
                     TFVRMYENGLIYRGPRIVNWDPNLKTNVSDDEVERKDTQSPFFTFQYGPFQIGTVRPE
                     TKFGDKYVVMHPDDERYTQYKHGDTFDCEWINGKITASIIKDEAVDPEFGTGVMTITP
                     WHDHVDFGIAERHGLDKEPIIDFDGKLLPIAQEFAGMGIDEARPKIMEKLREKGLLVN
                     TDSGYVHSKSFNSRGGGAIEPQIREQWFIDVNKPAVDWHGKKQSLKEVLIDVVRSGDI
                     KLVPERFDSTYFYWVENLQDWCISRQIWWGHRIPVYYKDGETLASIRPPEEFDGWEQD
                     PDTLDTWFSSALWTWSTLIDPELAKNYSLSLEDLLERSPDFQKFHPTNIMETGYDILF
                     FWVARMILMTTYMLQNVPFKTVYLHGLVRTRDGKKMSKSVPETCIDPLESIAEHGTDA
                     LRLALIQGTGPGQDLRLYPEKMESCRRFANKIWNAGRYILLTIPLGTPTAPPEKVHSE
                     LAQWLLHGLNDLIRHSQTGLEAHRLSDVVDSLRSFFWGEFCDWYLEMDKKPERTDEDN
                     QVLAYSYSTLLKLMHPYVPFVTEALWEQFKQPKMLAVSEWPQEQSYSFSESHNRIELL
                     REAISQIRTLREKANIGLDKKIGATLDSEKNAELFRTHQNLIIRLARLSELEINEKTP
                     DSSRDNLGAYFNETLASIEASAVDWKKEIESLQKKLKTESGFVEKSLKKLKNPGFLDK
                     APEKVIIELREKVASTEKTLEALKLQIHELDKLVE"
     gene            16370..17290
                     /locus_tag="EYO68_01045"
     CDS             16370..17290
                     /locus_tag="EYO68_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC06422.1"
                     /translation="MLKTAVIGVGYLGRFHAQKYAALAESELVGVVDVDSVQGQKVAD
                     EIGVPVFNDFHEVLDLVDAVSIVVPTVHHFETAKAFMEKGVHVLLEKPFAANIEEAEE
                     LEQLSRDKNLRLQIGHLERFNPVFTEFQELIDRPKYIENIRIAPFPKRGTDVDVILDL
                     MIHDIDLVLAVVGEYPESVEGTGVNIITSTADLANARLRFPSGCIADLTASRVSDKAE
                     RKMRIFQSGLYLSLDYGTGQARKLHVEPGTAVDPETLRPETFQLEKGDALLTEIESFL
                     LAVREGKNPKVTAEDGLNAMRVGWQIKNQL"
     gene            17266..18411
                     /locus_tag="EYO68_01050"
     CDS             17266..18411
                     /locus_tag="EYO68_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgP/YjgQ family permease"
                     /protein_id="HIC06423.1"
                     /translation="MANQESTLKYFMLLFRSIFIQLIVPFLLALGILTFILSMETVYR
                     LINLIVERGVSVFSVGLMLLYRLPQFLSVTLPLSIVISCVVLMVRLSTDNEISAMNSM
                     GISYWQIGRPFFLFGIVATGITLIITLWLQPAGYAAFEQEKLRVLKTQTAKTIQPLVL
                     NYDFPGKVLYVQDKDKNEDLSGVFITDLKLTQDSMVTLADRGRIEIREGERDLLLNLE
                     DGLIHLQGPADNYRTIAFEQFHYIFRPPQIVPSTKGGHIWGVPTKALLQNSSRSSKME
                     LFLRLTTPWACVAFAVAMVPLGLINIRQGRSGAYLRGLILVVSYYILWIGAKEMTMNM
                     NYQPYVLWMPPLLIWLFGLYGLNKSNLNLQNIFDILSSFGKTGSSVK"
     gene            18451..19104
                     /gene="lolA"
                     /locus_tag="EYO68_01055"
     CDS             18451..19104
                     /gene="lolA"
                     /locus_tag="EYO68_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HIC06424.1"
                     /translation="MKYFTFVFISILLFGFSGQSVSKETDLIQMIQEQYQSIQSFSGH
                     FVQTSYRNNTETVRRAEGLVSYKRPGKMRWLYEVPEEQLLVTNGETLWLFDPLLENVT
                     IQKLEKLTDGTALSFLLGLGDLQADFNRRLISQVLLTSPDALIVELEPKKAAANLSFI
                     QLAVHPVTYNLQIIALMDQEGNYRTIELESMHYNLVLEDNFFEFKVTQDMEVIEAGN"
     gene            19222..21279
                     /locus_tag="EYO68_01060"
     CDS             19222..21279
                     /locus_tag="EYO68_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIC06425.1"
                     /translation="MLKKELTLLNVYAIATGTTLSAGFFLLPGIAFNEAGPAVVLSYM
                     IAAIPLIPAMFSMVELSTAMPRAGGAYYFLDRSMGPFVGTIGGLGTWLALVFKTAFAL
                     IGMGAYLSIFWPEVPIVMLAVILAVLFGIVNLFGAKKTGTFQVFMVFALLLILLAFIG
                     KGIPEINSVHFEGFFDKGTVSIISTAGLVYISYVGVTNIASVAEEIQNPERNLPLGVF
                     LAIGTAIIVYAVGTTIMVGVLPANELAQDLTPVASTAEIIFGEWGKIAVTVAAVIAFA
                     SVANAGILSASRYPLAMSRDHLIPSKLAKLTARNIPHYGIAVTVVLIILLILTLDIAR
                     IAKLASAFQLLMFALICLAVVVMRESQIEAYDPGYRSPFYPWMQIFGVFSPLWLIAEM
                     GALPILFSLGLVTLGTVWYFAYAQNRVVRSGAIYHLFARLGEQRFEGLDRELRGIMKE
                     KGVREEDPFDEVVARAQVMEFTKEHQFGEITRQVAIEFERNLGVDAKELEQGFLKGSR
                     IGATPISRGAALPHIRLPDLKLAEMVIVRVNELCFVEAPDFSGKISLQGPIHAFFYLI
                     SPNENPGQHLRILAQIAGRIDDENFLKDWLDASDEQELKEILLRDERFLSLRIRNNTP
                     SSVFIGRSLKDIRMPEGCLVALIRRRGETIVPRGLTVLLEGDRLTIIGDAQGIEQLNN
                     KYN"
     gene            21284..22030
                     /locus_tag="EYO68_01065"
     CDS             21284..22030
                     /locus_tag="EYO68_01065"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIC06426.1"
                     /translation="MKTTLRTDGRKNNEERPLEISLDYTMHAEGSVLVSSGNTKIICT
                     ASVENKVPDWLLDKDKKPHHGWVTSEYGMLPRSTGSRRKRETGKIDGRSQEIQRLIGR
                     SLRSVVNLSVLGEHTIWIDCDVIQADGGTRTAAITGGFVALILALRNIFKTGNIKEFP
                     VKEYLAAISVGIVNSQPMLDLKYDEDKAAEVDMNVVMLESGEFVEVQATAEGKTYSRE
                     QLDLMLDLAVQGIHSHIAAQKKVLGNVLYG"
     gene            22051..22875
                     /gene="lgt"
                     /locus_tag="EYO68_01070"
     CDS             22051..22875
                     /gene="lgt"
                     /locus_tag="EYO68_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIC06427.1"
                     /translation="MYPYIFSLEIPWLNITLEPRFYGLFYAVAVLIGSQIVIAEIARR
                     RIKLDEDEAMNMTMLIFLAGLIGGRSYEVIFEWSNYYRFQPFWKVFAIWEGGLAIHGG
                     ILGGIVSLYFYCRIKNISLAAILDIGALCMILGQALGRWGNFTNGETAGPVTEFWTGI
                     VFPAGTAVDRYAHGAPVHPTMIYESLGNFLILALLWKLRLRNFRPGMLGALYLLSYSA
                     LRSLLTPLRMDNQYFVVADTKILAAYAISILLVGAGLFWIYQQKLWLPDEALSEKS"
     gene            22872..23873
                     /gene="cobD"
                     /locus_tag="EYO68_01075"
     CDS             22872..23873
                     /gene="cobD"
                     /locus_tag="EYO68_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobD"
                     /protein_id="HIC06428.1"
                     /translation="MNVDLVELLTHPAVILASIIGLDLLFGDPVYSWHPVRLIGNLIS
                     WFETKLRSIGLDGKFGGIILVLLLLFCAFVVCTGISHFLTRIHWSLSWLWSVYLGWSF
                     LALRDLLVHAKHVAEAMETENLSEAKRQVGMLVGRDTKLMDLPACGRATVESVSENLN
                     DGVMAPLLFFCFFGIPGMLLFKVVSTLDSMVGYQNKRYQDFGWCSAKFDDLLSWIPAR
                     ISWLFLSGTAALFPEFSGSNAFKVGWKDHAKLPSSNAGWCEATAAGALKIRLCGPIWR
                     EGKLAHNYWLGSQKDREGATAWDIQLVSRLALATTLIVSGILGYLLWLSEFKPFFSP"
     gene            23870..24367
                     /gene="cobU"
                     /locus_tag="EYO68_01080"
     CDS             23870..24367
                     /gene="cobU"
                     /locus_tag="EYO68_01080"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIC06429.1"
                     /translation="MKILFTGGVKSGKSRHAEKRVLEMSRGKKPVYLATTELLDPEMK
                     ERISEHRRRRGAKFHTLEEPLHLADTLLSTEVPVLVECLTMWLNNAMHYQLSEKKIFA
                     EIENLLLLECDVIFVLNEVGMGIIPDNSLARKFADLSGRIAQQLGEACDEVNLCVAGI
                     LLKIK"
     gene            24382..25488
                     /gene="aroB"
                     /locus_tag="EYO68_01085"
     CDS             24382..25488
                     /gene="aroB"
                     /locus_tag="EYO68_01085"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIC06430.1"
                     /translation="MKTLHVNLPGREYKIDIGLNILDQLLPEAVLSNSTDHVVVVTNT
                     TLHDLYPDRISQVLQNSGVRVSTCVLADGEEYKNLETLSLIFDFLMKFSANRKTLLVA
                     FGGGVIGDMAGFAAATFMRGIPFIQVPTTLLADVDSSVGGKTAVNHPSAKNTIGSFKQ
                     PEYVCIELSFLKTLPSRELKAGFIELLKHGLIHDVNLFEFIQGQTLEPLDFEFLEEAI
                     FRSCGIKGRVVEQDETETGLRAILNFGHTLGHLIETHAGYGTYLHGEAVGAGMCFAAF
                     VSWHCNELSEKDWERISSYLRKMLAPVVLHSLDQDVFRDLILHDKKAQKQAVNFIMLK
                     KLGESFIQQEMPVEKLWDEFKKFTALHPEFVELR"
     gene            complement(25496..26047)
                     /locus_tag="EYO68_01090"
     CDS             complement(25496..26047)
                     /locus_tag="EYO68_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIC06431.1"
                     /translation="MQLSEVPGILVAMPALKDTYFNKSVILLCRYDEEGAFGLVMNHP
                     TTTLVKEILSDEMKENVAADIPLLLGGPVQPESFWAVHSSDFSVEETTILSPKINLSS
                     AQDVLYSLANGQDVQSYHFGSGYAGWGAGQLDREIQEESWWLGPLDELLLLDLDYELR
                     WERTMDNLGFDPLTTTFSQTGMV"
     gene            26075..27142
                     /locus_tag="EYO68_01095"
     CDS             26075..27142
                     /locus_tag="EYO68_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06432.1"
                     /translation="MIMPSLPQQKKDHNLVTQFLRSIHYKSSSGQKPYGNALSILRAI
                     FEASGQQHLLYLVKLRKLWFAEIDSFMARNSYPRNISVLRQFTVNDDFCQELAKASVS
                     PDFLQAVEKLNGESFKRSEQLNGQLQKRLKRLLSPEESELLKHKVRFKHLQTILHLTV
                     YDGSIAQALRFETEAYLRIFHRLLPELKLDSINCHVGDLGQAQYDQRQVALLAKDWNQ
                     VTPPEVHERCMPAFLHRVSRGHAVLVLYVSTEEGLEYLKRTPGSDWLVQHLHKKRTEL
                     QEVLQRIAFVPKPELDLEQIRLRSVVLGEIDSEKLSSVTEITSRKSTASTSKTTLSAK
                     EQFAKIRMHLNEGTSDAVNSA"
     gene            complement(27139..28035)
                     /gene="rsgA"
                     /locus_tag="EYO68_01100"
     CDS             complement(27139..28035)
                     /gene="rsgA"
                     /locus_tag="EYO68_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIC06433.1"
                     /translation="MTQTGTIVRHTKSFYYVDVGEIEPRLCRIRGNLFKESSYINKIA
                     VGDEVEVDLTAAEDAGWILRMFPRRTKLSRRPSVGYPEQVLVSNADTLLMVASMRTPP
                     FRGGLVDRLLVAASCGGLEPVLILSKADLATSDEISPIENLYSSLGYTILVTSIPESR
                     GLESLRDLLQNRTTVLSGHSGVGKSSLIKALFPDWEIRIGRISNKSGKGRHTTRMAEM
                     FRIPIGGFIVDTPGIRELEPLVTPNELDSHYVEFVPYLGQCKFKGCTHIHEPQCLIKD
                     AVASKKITEQRYKSYCTLFESL"
     gene            complement(28058..29554)
                     /locus_tag="EYO68_01105"
     CDS             complement(28058..29554)
                     /locus_tag="EYO68_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIC06434.1"
                     /translation="MVNQPVLIVDDEVEMRIAMSETLKHCGYPVEISHNAIDALKKFK
                     QNEYSLVITDMTMPKRSGLELLKDIKSISPAKPVIMATAYGTVETAVEAMKHGAFDYI
                     RKPINFDSFQFIVQQALNYKGTPGTAAPVAESKTSKSKPINVTKEIITQNQTMLDLLK
                     VAKNVAKSKSTMMIQSESGTGKELLAYYIHENSDRAKRPFVAINCAALPETLLESELF
                     GHAKGAFTGAIQDYRGKFEQAHTGTILLDEISEMALPLQAKLLRVLQEYQIDKVGGKE
                     PIPVDVRVIATTNQNLLEMIDSGKFREDLYFRLNVIPLALPPLRDRMDDVPVLVTYFI
                     KKHSQINKRNCPAVSRETLNILTNYHWRGNVRELENVMERALLLCNGEEIQPSHLLMS
                     SQLGETTINEDLAAITTDYSKTPASYSSQTSFTFSEPQNSPDNGLGIEVGMSMKEAEK
                     RFIFKTLHETKGNRTHAAKTLGISIRTLRNKLNEYRAEGTDFELEPEE"
     gene            29893..30159
                     /locus_tag="EYO68_01110"
     CDS             29893..30159
                     /locus_tag="EYO68_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HIC06435.1"
                     /translation="MSLEEDFNSAAERATQLPKRPPNDIMLQLYALYKQANEGDVTGD
                     RPGFADFEGRVKFDSWNKLQGKSGEDAKQEYISLVEKLEQEVAE"
     gene            30167..31648
                     /gene="gatA"
                     /locus_tag="EYO68_01115"
     CDS             30167..31648
                     /gene="gatA"
                     /locus_tag="EYO68_01115"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIC06436.1"
                     /translation="MPFDLSLIDLVERIKSGELRAEAVMEAVYSRINQYNSSLNVYRS
                     LLPREIAISRARIIDEEISSGKTLGKLAGIPVSIKDNICIKDPELSNACGSQILGEYH
                     SPYNATVVEKLLQEDAVIIGTTNMDEFAMGSSTENSFFSPTRNPWDTERVPGGSSGGA
                     AVSVASGMALLALGSDTGGSVRQPASMCGVSGLKPSYGTVSRYGLVAFGSSLDQIGCL
                     APSAADCRYAFSIIHGHDPHESTSAKIELSTAPDVAKLSGLRVCLPDEYVAAESFDPD
                     VVGSVHELEALLQKHGATVERRSLDFLKYVIPTYYVLAFAEASSNLGRFDGIRYGIRK
                     QDQPSLKSLYTSTRKKGFGVEVKRRIMLGTFVLSSGYYDQYYGRAVQVRAWMEKQVAL
                     LFEEFDFILGPVSPFPAFKLGEKNEDPLAMYLADICSVLANLTRTPAISIPGRPTSDG
                     LPVGLQLMGRRFEDHNLLATASAIQELTAFHKLRPEKVFATKP"
     gene            31716..32984
                     /gene="serS"
                     /locus_tag="EYO68_01120"
     CDS             31716..32984
                     /gene="serS"
                     /locus_tag="EYO68_01120"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIC06437.1"
                     /translation="MLDLKIIRSQPEVIAENCRKRNVDVDIEKLLALDEQVRQITSEV
                     DSVRQRRNEISNKMKVKIPPEERQSLIEESKNLREEESEKDSILRELLEQRLDLHKQV
                     PNLTHPESPEGFTDEENVPIREVGTKREFDFEPKDHVELMEALDLIDFDGGAKVSGQK
                     FYYLKNQAVFLELALAQYALSLLQKEGFTVHITPDLARHQILDGIGFNPRGAETQIYS
                     VEESDLCLIATAEITLGGLLIDQVLQPEQLPILYAGLSHCYRTEAGAAGRESRGLYRV
                     HQFSKIEMFAFTHPDKSEEMHEKMREIEEQIYQDLEIPYQLVNICAGDLGGPAFRKYD
                     LEAWMPGRGKWGEVTSTSNCTDYQSRRMNIRFKDPETGKNRFVHMLNGTAIAVSRTLI
                     ALIENGQQKDGSIKLPRCLNIKDIPAPASG"
     gene            33123..33587
                     /locus_tag="EYO68_01125"
     CDS             33123..33587
                     /locus_tag="EYO68_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIC06438.1"
                     /translation="MPIGEVPPTIVLEGDLGGRLDGTQWSSEELVSGKVLVLFYVDPD
                     ESDLNNHVSEALKAEKYPPENYGSVAVINMAATWLPNFAINIKLKSKQEEYKTTVYVK
                     DLEKTLVQKWGLSDDNSDVVLFSKDGKVLYSVDGKFTDTQVKEIVKTVWDNL"
     gene            complement(33621..34079)
                     /gene="rnhA"
                     /locus_tag="EYO68_01130"
     CDS             complement(33621..34079)
                     /gene="rnhA"
                     /locus_tag="EYO68_01130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIC06439.1"
                     /translation="MLKTSVTIYTDGACSPNPGIGGWGAVLISKEHNLRTELSGSEVD
                     STNNRMEMTAAIKALEALKKSCVVKLYTDSSYLKNAFEKNWLNSWQNNGWKSKARKPV
                     ANKDLWLRLLEVSKKHEVSWHWVKGHSGNPENDRCDELAVQARKKFAKSN"
     gene            34191..34523
                     /locus_tag="EYO68_01135"
     CDS             34191..34523
                     /locus_tag="EYO68_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06440.1"
                     /translation="MNIDFKQFGTWIYVDPDEQSIELLEETLNLDMLCELLRCESTDM
                     EELGGPFLAYFDGEGASQERQTEWEFNENPCWGPILIVKIGKEDSRLETCDEDDLAVI
                     EPLVKFLD"
     gene            34545..35543
                     /locus_tag="EYO68_01140"
     CDS             34545..35543
                     /locus_tag="EYO68_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIC06441.1"
                     /translation="MNVSTFRALVVSKTDEKTFTREITERSISDLPEGEVLIRVHFSS
                     LNYKDGLSCIGNPGVTRNYPHTPGIDASGKVTESSDSQFKTGDSVIVTSYDLGMNTSG
                     GFGEYIRVPADWVVPLPEGLSFKDAMVYGTAGFTAALSVHALQKHGVSPEQGEIVVTG
                     STGGVGSISVALLSHLGYNVVAATGKTNETEYLSGLGAKEIIHRDEVKDDSNKPLLRE
                     RWAGAIDTVGGTTLATLLKATKRGGAVASTGLVDSADLPTSVFPFILRGVSLLGIDSG
                     FTPTKLRREIWNKLAGDWKLSQLGQLKIDCNLEGLDPEIDKILAGGQRGRVVVDLQ"
     gene            35583..>35800
                     /locus_tag="EYO68_01145"
     CDS             35583..>35800
                     /locus_tag="EYO68_01145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503593.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC06442.1"
                     /translation="MSDTIKYLLPETEIPKDWYNIVADLPEPPPPVLHPGTHEPVGPD
                     DLAPLFPMSLIMQEVSGERYLEIPGPVR"
BASE COUNT        10410 a         6777 c         8207 g        10406 t
ORIGIN      
        1 gtggagcaaa gttatgagca aatccaaatt tagtgaaaaa attggtactg aaccttccgg
       61 atggtttgag ggggcaaggt tgaattttgc agaaaatttg ttggcacagg gtaatgatga
      121 aaatgaagca ctctgtttta ttggtgaaaa tggttcaaca agctccctta gttacgaaga
      181 actacggaat gcagtggcac aatgtgcttc cggactgaag tcccttggag tttcaaaaaa
      241 tgatcgtgtc gcagcagtta tgccaaattg tccggaagcc gttatcggga tgctggcaac
      301 tgcgagtctt ggagcaattt ggtcttcctg ctctccggac tttggaatta acggcattaa
      361 cgataggttg ggacaaattt caccaaaaat tttgataact gtcaacgcat atcactacaa
      421 cggtaaagaa catgattgtc ttctcaaaat ccgagaaatc gtcagtcgta ttccttcaat
      481 tgaattggtg tttatagtac cttttacaga aacaacaata aaatttcaag gtaatgagca
      541 ggcatggggc aaattactga actcttctgc aggtactgta aattttgcac aacttccttt
      601 tgatcatccg ctttacattc tttactcttc aggaactacc ggtttgccta aatctatcgt
      661 tcacggagca ggaggtactt tgctgaagca tctcaccgaa catcgttatc atgcagatgt
      721 gcatcccaga gaccggatgt ttttttttac tacttgcgga tggatgatgt ggaattggct
      781 ggtaagcggt cttgcttcag gagcgactct aattttgtat gatggtgcac ctggattccc
      841 ggacattggg cgaatgtggc gcttaataga cgaacagcag atcacacatt ttggaacaag
      901 tccaaagttt cttggtgctg tcagtaaaac cggatattcg ccaaaacaaa atcatcagct
      961 ggaatcatta cgtgcaattt tatcaacagg ttctccactt ccggcagatt tgttttcatg
     1021 ggtttatgcg gaagttgctt atgtcccact aatgtcagtt gcgggcggca ctgatattgc
     1081 cggatgtttt atgggaggta acccgaatct tccggtttat tctgaggaac tacagtgcaa
     1141 aagtctgggt atgaatacac ttgcatacaa ttcagacggc gagtcagtgg tttgtgaaaa
     1201 aggagaattg gtttgtgctg acccttttcc gtctatgccg atttatttct ggaatgatgc
     1261 ggatggtaaa aagtatcata aagcatattt tgaaaaattc ccgggagttt ggacacatgg
     1321 agattttatt gaaattactg aacgcggggg cataattgta tatggcagaa gtgatacagt
     1381 tcttaacccc ggtggagtta gaattggtac tgctgaaata tatcgtcctg ttgaagaaat
     1441 gcaggaggtc gaggacagtt tagttgttgc acagccttgg gagagtgatg tgaggattgt
     1501 gctttttgtg gtattacgtg acggaatccg gttggatgaa aatttagttg ccaaaataaa
     1561 aaccgttatt cgcagtgaca ctactccgcg tcatgttccg gcacggattt tagcgattcc
     1621 ggatgtgcca agaactatca gtggaaaaaa ggtggaaaag gctgcactgc aaactatcaa
     1681 aggcgaatat attgaaaata catcagcact tgctaatccg ggaagcttgg aacatttttc
     1741 aaaattaagt gaagaattga gaaaataaga taataataaa gaggctaggt ctctactttt
     1801 tgtgagtctt cgtatatgcg gtcttttagg ctctgaagca gatcaagaat cggcaaatca
     1861 gggacttccg gccattccat cagtttcagc aggtattttt tcaggcgtcc ccgtgacaat
     1921 ccacgtttct cccagctgta ataaatagcc tcaatttctg cagatagagg tctcccatcc
     1981 aaacttcttc gtaaaaggaa tctgagtgtg ctgtcaggat actggcgaat aaacttttct
     2041 aatgcagcct ttgaaatttt ttctccgcca aataccacat cagtctcgtc agaatcaact
     2101 agaatcaagc cgctgtcatc ttcttcaatc tcttcatctt cttcatcctc ttcaaagtta
     2161 agcaacagat cggcagtagt aacagttgag gaatctgcct cagtttctgg ttcgtaatta
     2221 tttttttctt tcaactcatc ttcttcttca acctcaactg cagcatcttc aatagtttga
     2281 gatgccacag tttcttcctc aaaaattccc tcttctgctt tttttgactc tatcaagact
     2341 tctgcagttt ctgctatatc cgtttccaag tcaggctcta gaggaaattc tgaaagttca
     2401 ttctgatctt cagtcagtgt tgaactgatg tccagtttgt caacaatatc atttttttct
     2461 tccgcaataa gaatttctgc aacatctgga gcggttcgtt tttcagcagt tggatttagt
     2521 tgtgaaggtc gcaaaaattt tattggagag ttttttcgct cctgttttat tacaggattt
     2581 aaactttcga ctgaaacacc acgttttttt gcaatgtgct tcaggaagcc ttttaccagt
     2641 tcacgtcctt caagtcgttc ccggacctgg cttcttaact gtttcaagtc ttcaatcttg
     2701 aagttggttt ttgaaaaatc cggttcctct tcctggtctt cctgttccag tgtctctcca
     2761 cctaaaacca gactataata cgtctcgatg tgcaaaagga aatcatacaa ggggtgtttt
     2821 tcttgctcac ggagtccata cgaaatgttg taacgaatat tgaaatgatg gtgtttccct
     2881 ttagccagtt gatcaaatga tttctgaatt cgttgagaaa gttttttttg cagaatttct
     2941 gcaggccaat taaattgttt aacataatca atgaaaccca cataacgctc tcttggaacg
     3001 agtgtacgaa gtacctcagc ttcctgatca aaaaagtcac gtatcaccca tcctcctgct
     3061 tcaagctcat tttcagaagc atccacaaag acaaatcctg agttgaaaag atgctcgcac
     3121 aactgaataa gaagtgaatc ctgatctgga agcagcgaat caaaatgttc actgtattcc
     3181 ctgaaggtag cattacggtg catgagacga aaagagttta atcagattag aaatttggac
     3241 acaacacaac atagcgtaaa atcttgcgcc gggcaaccac agagtgccag aaacagggaa
     3301 agattaattt tagatatttg ttttagtagt tttgtaaaga cttcaaatta gaattgcgtt
     3361 gagattcaac aagttttttt ctaagcttat tgtttgagaa tcattctgac attaagacga
     3421 aatactgccg attcttcttt gttatacaga aagcaataag ggtagtaatc taacttttaa
     3481 agtaatttcc aattttaaga taattaatta ataatgatca gcattgctga cttagccatg
     3541 aattatggca aaaaaacgct ctttgaaaaa tcatcagtca cctttaatcc aggtaaaagc
     3601 tacgggcttg ttggtgcaaa tggttctgga aagtccacgc tgttgcggct gattgctgga
     3661 gaagaaaaac ctaattccgg aacaatatct gtaccacgtg acttgaaact tggagttctc
     3721 aggcaggatc attttcagta tgagaatatt aaagttattg acgtagtgct cctcggaaag
     3781 cccgctttat gcgaagctct tagagaaaaa gaaaaaattc ttgagtctga aaatctagat
     3841 agtcaatccg gtcacaggct gggtcaactg gaagagatga ttgctgaagc agacggttat
     3901 gtagctgaac cttttgctgc agagttgtta tctggattgg ggattggtac aggatttcac
     3961 actggtccta tgagtgcact ttccggcggc ttcaaattgc gtgtactttt agctcaattg
     4021 ctttttcagc aacccgacgt gctgctgctt gacgaaccaa ccaaccatct ggacattgtt
     4081 tcaatccgct ggctggaaaa ctatttgtgt acacagttcg aaggaaccct gatttttatt
     4141 tcacatgaca gaaattttct caatgtggtt gccaggtaca ttattgatat agattacgaa
     4201 gaattacgac tttacactgg aaattacgaa cagttccttg aagcaaaaac cctggctgaa
     4261 actcagaaac gtaaagaaat cgaaaactat gagcgcaaaa ctgctgaact acaggccttt
     4321 gtagatcgtt ttcgtgcaaa agctaccaaa gctcggcaag cccagtcacg tgtcaaacaa
     4381 ctcgataaaa tggaggtgcc agaggtaaag cgttcctccc ggatatctcc agacctgagc
     4441 tttgagcagt ttcgaccctc cggaaaaact gtgctgactg taaaagatgt gagtaaaaag
     4501 tttgggaaca ctgaggttct gaaaaatgta tcttttgaaa ttcaacgggg tgaaaaagtc
     4561 gctatcattg gtccaaacgg tattggaaaa tcaactttac tgaaaattat tcttgctcag
     4621 ctttctgcag acgaaggtac tttcgagtgg ggttatgaaa ctcatacctc ttattttgcc
     4681 caagatcacc atgaacattt acgtggaaaa atcagtgctt acgactggct ttatgcaact
     4741 gcaccacacg aaccgattgg agctatccgt ggtttgttag gtcgagtttt actgtccgga
     4801 gatgaagcgc ttaaacaggt tactgcattg agtggaggag aatctgcccg tttgctattt
     4861 gcaagaataa tgctggaaaa aaggaatatc ttaatcctcg atgaacctac aaatcatatg
     4921 gatctggaag gtgttgcggc acttggtgat gctttgaaag catttgaagg tactgttttg
     4981 gtggtaagtc actatcggca ttttgtttcc aaagttgcaa ctcatatcct ggaattaact
     5041 ccacatggtg tccgcgactt ttccgggagc tatcaggaat accttgaaaa atttggtgat
     5101 gactatctaa gcagggatca gcctctttct acgacacacc gggaaacaaa agcgacacct
     5161 tcaactcagg aattgagcta tgctgaacga aaaaaacttc agagtgaaat atcaaaatta
     5221 aaaaaacagt ctacacgttt tgaaacactg attgaagagg gtgaacaaaa aatttctgca
     5281 attgaggcaa aatttggagt tgaaaatttt tatcagcaag catcgcctga ggaaatacag
     5341 aatttacaaa ccgaaaaaga aaaattgagc gctcaacttt ctacacatct cagccagtgg
     5401 gagacttcct cacataaatt ggaagttgcc caagcacgtt tcggcggtta atttttcaaa
     5461 aaaagtaacc agcagttttt acatacgccg tttatttagt agctgaagat ttagaacagc
     5521 tttactaatg aggtttaaac atgttaacga tgttgtactc ctcaatcagg cagattttag
     5581 attgttggca aaattgatgt gagtaatgta tgattggtta tacaactaac ttaagaggtc
     5641 cccatgcgaa agagtaattc aatttttaaa ttcggttttt tagggttgat cttaactttg
     5701 cttcagatac ttcccctgaa taatacttcc ttttctcaaa caactccgga aaaggctgca
     5761 gagttatgga atttacgtca gtcagaaatt caacagcttt atcaggaagg taatcttgaa
     5821 ggagccttga attcagcaca ggaatcagtt tcactggcag aagtagcttt tggttcaggt
     5881 gcattaaaaa ccatttccag tatgttgttg caggcacaaa tccatggaga attagatcaa
     5941 ctggaagagg cgaatcagat ttaccaggta gctcttgaaa cttcagttgc tgcttttggt
     6001 gaaagtgatg cgactacact ttccgttctt gacaactatg gtggatttct caaatcaatt
     6061 gatccggaaa tgtcagaacc ggttttacag gaagctctta ggcttaccac tgaagatgat
     6121 ccacaacgtg ctgccagatt gagagctctt ggtctgaatt tacaggaatt gggacgtatc
     6181 ggtgaagcag aaggaatgct tagtgaagca ctgaaagctt acaaaaaggt cttgggcgaa
     6241 aattctccgg aaactctatc ttcaatggct gacttagcac agctgtacct gacacagggg
     6301 aaattaaaac ctgctcagga attgtttgaa acggcattgc ctttaatgct agaggttatg
     6361 aataaaacga gttttctgat tttggcgagc aaggaaaatc ttgctgaaat ttatcgtcag
     6421 caaggaaaat tttctctggc cgaaaaattg tttttagaga gtatgcaggg tgcagttgca
     6481 gaatatggtg aggaagattc tcttgttata caggccagga gtcatcttgc ccagttatat
     6541 gaagatacgg gcaagctaaa aaaggcattg cttttacatc agcaggttta cgaggtcgac
     6601 ttagcattgc ttggagaggc gcatccaaat acagccggcg acctgaataa tttggctagc
     6661 gttcggcgcc gcctgggtga ttatcaaaaa gcagaaaaac tttttagaca atctttaaaa
     6721 gtgatgattg ctgcacttgg tgaagaaaca ccacagacag tttctgtaat gaacaacctt
     6781 gccttgttgt tggaaaatca gggtttgtac gatgaagcag aaccactttt caaaaaagcc
     6841 tttgcaattt ccggaaaaat tcagggagag aaacatccaa cctcattggc tttgctgaac
     6901 aaccttgctt ttcttaatga atctcaaggt gactttattc gttctgaatt gaattataaa
     6961 aaagtaattc aactcaacac agaagtattt ggtgaaaatc acacaaacac cattgcaaat
     7021 caaaacaacc ttgcctatct ttatttacgg gatgaacgtt acaacgaagc ccaaccaatc
     7081 ttcaccaaag ttcactctca atggagcaaa ttacttggtg cagaacatca aaatactttg
     7141 aaagccctta ataacctggg gcgtgttcaa aaatcacttg gaaatttgga agctgcggaa
     7201 aaacatttga tggaggcttt ggcaggaaga accaaggttt ttggtaaacg tcatgccgac
     7261 actttgcgca gtatgaatga tatgggtgag ctttttgtgg tgcaggaaaa gttggacgag
     7321 gcacttaaaa tgtttcagga aacgctaaag ctggaagaag aagttttggg cgaaaatcat
     7381 ccttatactt ttgaaactct gaataacctg gcagatttgc tcaaaattat gggtaaaacg
     7441 gaagaagcat acaaattgtt tatgacaggc tttgaacgca gaaatgcatt tttggatcgg
     7501 gtgctttggg ttgcgggaga caatacacgc caatcttaca tcagtttgca ccgaccggaa
     7561 caaaatgctt tgattcggat gctattgagt ttggatgacg aacgtaccgc gcgtgatctt
     7621 tttgaagtga gtttgcggcg taaaggtatt ttacttaaaa ttaccagtga aactcaacaa
     7681 gtcttaatgc tgtctggttc acctgaattg caggcaataa cggaagactt gacccaaacc
     7741 cgcaaaacat tagcaggact cacattgtcc ggaccaaccg atgaaaaccg gaaggagttt
     7801 ccaaaaatca tttttgattt ggaggaaaaa gttaacatgc tccaactaaa acttggagag
     7861 caaagtgtgc gtttccggca gtccacaaag caagttacag tggacgaggt agttgaggga
     7921 atgggagaaa atgcaatggt tgattttatg agttttcgcg gcgaggatga ttcactgcgt
     7981 atgttagcag ttgtagtaaa tgcaggagag ttcagttttg ttgaatacga agatttagaa
     8041 ttgatcagtg ctattgtcat ggaattgcga gaaattgttc aggatgaggg tgcagaagat
     8101 gaggacatta aaagcgttgc ctatgatctt tgggagattc tttggcaacc actcacagaa
     8161 tcattgggag aaacagcatc gatttttatt tcgccagaca gtctcttaaa tgttttacct
     8221 tttgatgttt taacagattc tgatgacagt tatttgctgg aaaattttga tttacgcatc
     8281 ataagttccg caagagattt ggctctcgat ccattgcttg ccgcaaaagg tgatttgatg
     8341 attattgcgg ggccggacta tgattctgat aaaatcctta aaactccagc agctcgagaa
     8401 gtaactcaca aacgatcccg aagtgttgca cgaggtgctc gcatgggttc cggattgcgc
     8461 ggactgaatt ttgacccgct gcccggagca gaaaaagaag gagaagttat caaggaagtt
     8521 tcagacacaa aggatcggaa caccaccatc ttttcacaaa gagttgcaga agaaaaactg
     8581 ctccgggaaa tgtctgcacc tccagaagtt ctacacattg caactcatgg attttttctc
     8641 aaagaagacg aaaagttgaa aaaacgcctg cagggtttaa gtcggggctc gagcagcgtt
     8701 ccgccgcctg cggataatcc gttgctgcgc gcaggacttg cttttgccgg tttgaacgcg
     8761 aatgcttcca tgcttgggga aattgacacc gacaacgatg gagttttaac agctatggaa
     8821 gttttgagtt tggatttagc cggaacccaa ttggttgttc tttctgcttg cgaaactggt
     8881 ttgggagaga ttcacgaagg cgaaggcgtc tatggtttac gccgctcttt tcaggaagcc
     8941 ggagtaaaaa acgtgatcaa ttcattctgg gaagtcagcg atgccggaac gcaattattg
     9001 atgaccaaat tctatgacaa attccttgct gggatgccag cccgggaagc aatgcgtgaa
     9061 gcgcgtctgg aaatgctgga tgatgttcag tggagtgctc cattttattg gtctgcgttt
     9121 gtgatggttg gacgtaatac ttaaaaacat ggagcaatta gtcattcatg gtggagcagg
     9181 aagtctggaa gggaaaacga ctgaagctca gaaaatgcat gattcccttt gtaagatatg
     9241 ggaagagaca tttgaagtac ttcaaaaacg ctctgcagaa gatgccgttc ggcatgctgt
     9301 ccggatgctg gaggatgatc ctgtttacaa cgccggaact ggatccaagc ttcaggcaga
     9361 cggtcagata cgcatgagcg cagccttaat ggacggcact aataatcggt tttctggcgt
     9421 gatcaatgtt caaaatatcc ggaatccaat tgaagcagca gcattgctgt ccggagaaaa
     9481 acattctatt ttaagttctg cacaggcaac tgaattctgt cgaacaaaag gcattcccga
     9541 atacaatccg gaaactgagt ttcgcctgca cgaatacaaa agtaattgtg ccggaattca
     9601 cggaacagta ggagccgtgg cgcttgacaa agatggacat atttttgccg gaacatcaac
     9661 tggcggaatc ggttgtgaaa ttcctggacg tgtcagtgac agtccaacag tagccggaac
     9721 atatgcctcc acaaaagcag gtgtttcctg tacaggaatt ggtgaacaaa ttacccagca
     9781 agccgctgct gcaaaggtag taaccagggt tggggatgga atgttgctga atgaggcagt
     9841 taccagaaca atgcatgaaa gtgacgactt caattattta tatgggctga ttagtctgga
     9901 ctcggagggt cacgttgagg tgggtaaaac agctgcgctg agtgctgttt tctatgcatt
     9961 tcacgatggt gaaaaaatca gaacattttt tgagtgatta attttttgaa gttcagttgc
    10021 ctgccgaata tttgctgcgc tcattatcta accattctcc gataaaaagt tgttgagaat
    10081 ctggcatctg ataacgctgt tcaagaatga aattgtccaa aaatgggcca gtttgcagaa
    10141 gaaattgatt ccgccacatt tcactttgtg acaattttgc atcaaagtgc agtttgccaa
    10201 ttccaatttg tcgtctaaaa cgaaaacgct ccatagtgta accggatgcg acaaaaacta
    10261 attcaatcgc tgaagcttcg agatcaaagt gtattcgaaa acgtccatcc tcttccggaa
    10321 aaaccagcct tgcctttgga gcataaagag gggattcgtt tttaaattga ataaattgcg
    10381 aataatattc aagaacaacg acgaatcctt tggattttag gtcatttccg ttttcttcca
    10441 gtaaagtacc atcaatccac tgtgttcccc atccggggcg atagttcagt gattccggat
    10501 aacatcctcc tgacaaagtt agtatcaaca gcagatacag aataaaaaat ctcattcctc
    10561 tgggctctct ataaagattg aatctatctt gaagataagc taaaccccca gaattgcaag
    10621 ttattgttca ggaatttaca gtgttgccaa aactttgtag ttcttgaccc tgcacttagg
    10681 ttttgctaaa cttacacgat tggattttag ttagactgag tttgcaaatg agagaattat
    10741 ttttcataaa attatcactt ttcattacat aatcaaacaa actccgtctt ctgtcctcac
    10801 aatgcccaat gtggtaaaag gaatctatgt ggaaaaaagt taaaaatatt tttggtgatg
    10861 aaaacgcaaa aacactgaat ggttaccgga aagtggcgga caaaataaat gcgcttgaac
    10921 cggaaattca ggccttggca gatgaagaat ttccaaaaaa aactacagca tttcaaacac
    10981 gacttgaaaa aggagtagct ctggatgagc tgattccaga agcttttgca ctcgttcggg
    11041 aagcctcact gcgtgttatt ggagaacgcc attacgacgt tcagttacag ggaggtttgg
    11101 cgctgcatca cggaaacatc gctgaaatgg caaccggtga aggaaaaact ttgtcttcaa
    11161 cctgtcctgt ttatctcaat gcacttactg gaaaaggtgt acacatcatc acgcctaatg
    11221 attatctggc gaaacgtgac agcaaatgga tgggagcaat ttttgagttt cttggtttgt
    11281 ctgtcggctt gattcagcat ggaatttatg atgaagaacg ccgcgctgca tatgaagcag
    11341 atattacata cggtacaaac aacgagttcg gttttgatta tttacgcgac aacatgaagt
    11401 tttccctgga ggattatgtt cagcgagaat ttaactttgc agttgtagat gaggttgaca
    11461 gtatactgat agacgaagca cggactccgt tgattatcag tggaccaact gaagataaca
    11521 tagataaata tcatcaaata aataagttcg tatacggact cagtcgtgaa atccgtaagg
    11581 aggaggtggg caaactacct caggatcaaa tgcacaatat tgccgaaaat ctggaagaac
    11641 tggaagacca cgatattgtg cgtgatggag attatgcact cgatgagagg gcccgcagta
    11701 tcagcctgac agaccagggt tctgtaaaaa ttgaagggcg tctcagggat cagctggtta
    11761 aagatacaag tctgtttgat tacgaaaaca tggaaatttt acaccatgtt aaccaggcac
    11821 ttaaagcgca ctacctgttt aaaaatgatg tcgattatgt tgttcaggaa ggtcaggtaa
    11881 tcattgttga tgagtttacc ggacgactga tgccaggacg gcgctttagc gacggactgc
    11941 accaggccct ggaagcaaaa gagggcgtca atgttgagcg tgaaaaccag acactggcga
    12001 cgataacttt ccaaaattat ttccggttgt acgataaact ttctggaatg accggaactg
    12061 cggaaacaga aaaaaatgaa tttaagaaaa tttacaacct gggagttgtt gtagttccca
    12121 caaataaacc gttgattcga aaggatcttt cagatgtagt tttcaaaacg gttgatgcaa
    12181 aatatcgtgc aactgttgat cgtatcaaag tacttaacga aaaggggcag ccggttttag
    12241 tgggtacagt ttccatcgag gtgtctgagg ccatcagtaa attgctcaag aaagcaggta
    12301 ttgaacacga agttcttaat gcaaaacatc atgaaaggga agcggaaata atctcaaatg
    12361 caggccaatt tggggcagtg actattgcaa ccaacatggc tggtcgtggt acagacataa
    12421 agccttcacc tgaaactctt gaagtaggtg gtgtgtttgt tttaggtaca ggaaggcatg
    12481 acagtcgccg aatcgacaac cagttccggg gacgttccgg acggcagggg gatccaggtg
    12541 catctcagtt cctgctctca cttgaagatg atttgctgcg gatatttggt ggagaacgta
    12601 tttcaaactt gatggataag cttaacattg aagaggatga accgattgag catgtattca
    12661 tcaccaaggc tattgggaat gcacagaaga aggtggaagg ttatcacttt gatatccgga
    12721 agcacgttct tgaatatgat gatgtgatgg aaaagcaaag atccatcatc tatggcaggc
    12781 gcagagaaat tcttggagaa gaggtgcaga atctgatttt agaaatgtgc gatggtgtgg
    12841 tagaccaact gatggagcag cattgtcagg ataaatatgt agatcaatgg gatgttcagg
    12901 gattcaaccg tgcttttgaa ggcgtctttt caaaggttcc aaacgaaaaa tggtttgaag
    12961 atgaactaaa tccggatgaa tattcagaaa aattttatgg ttggattgaa gatttgtaca
    13021 aagaaaaaat tgagttcttc cgtaatgttg cagaacttaa ttttgagccg gatgtttcta
    13081 ctgaagatcg caaagaaata ttgggccaaa tgatccttga cctggaaaga caaattttgt
    13141 taaaagttaa cgataatctt tggaaagatc acctgctttc aatggatatg ttgcgggaag
    13201 gaatcggcct cgttggttat gctcagaaga aaccgctgga cgaatacaga aaacaggctt
    13261 ttgaaatgtt ttctgatttg atgaacagaa ttgataatga ggccatcagc acttttttca
    13321 aattaacaat tgcccgcccc attgcagaac ctgaaccggc accgttactg aaggatgtgg
    13381 aattcataca tggagaagtc gagataccgg acggaaccga aacaaaaaag aaaaaaccac
    13441 aacctgtccg tgcacagtcc actgtaggac gcaatgaacc atgtccttgc ggcagtggta
    13501 aaaaatacaa aaggtgctgt ggacttgcca aaaaaattgc ctgaacgttg atgctgattt
    13561 gtactcaaaa cgaggtactc ccagctgttt tcacagttgt agcttcccaa tcttcatccc
    13621 agttttatta aaataggtaa atgacttcat tagccaaagc ttacgacccc caagcttttg
    13681 aatccaaaat tgcagaacaa tgggaaaaca gcgatgcgtt tcgcgccgat gcacattcca
    13741 acaaacctgc tttcaccatt tccatgccgc ctccaaatgc caccggacaa ctgcatgtcg
    13801 gtcatgcagt gatgctggct ttggaagata tttttattcg ctggcaccga atgcagggcc
    13861 atgaagcact ttggctaccg ggaaccgatc atgcagccat tgccactgaa aatgtggttt
    13921 tgcagcaaat taaaaaagag gaaggcattg ctgatccacg ggaagtactg ggacgtgaag
    13981 aaattttgcg gcgtattgca gaatatgtgg aaagctccca gggtaccatc cgcaatcagg
    14041 taaaagcgct gggatcaagt tgtgactgga gccgcgaacg ttatacgatg gatccgcagc
    14101 tcaatcgctg tgtgaatgaa acttttgtgc ggatgtacga aaatggattg atttatcgtg
    14161 gaccacgtat tgtaaactgg gatccgaatc tcaaaaccaa tgtttcagat gatgaagtag
    14221 aacgtaaaga cacgcaatca ccatttttca cctttcaata cggccctttc caaattggta
    14281 ctgtacgtcc ggaaaccaag tttggcgaca aatatgtggt gatgcatcct gatgatgaac
    14341 gatatacgca gtacaaacac ggcgatacat ttgattgcga atggatcaat ggaaaaatca
    14401 ctgccagcat catcaaagat gaagccgttg atccggaatt tggaaccgga gtgatgacaa
    14461 ttactccatg gcacgatcat gttgactttg gtattgcgga gcggcacggt ttggacaaag
    14521 aaccaataat tgattttgat ggtaaattac ttccaattgc acaggaattt gccggaatgg
    14581 gaattgacga ggcacgtccc aaaattatgg aaaaacttcg tgaaaaagga ctgctggtca
    14641 atacagattc tggatacgtg cacagtaaat cattcaacag tcgcggtgga ggtgcaattg
    14701 aaccgcaaat tcgcgaacaa tggtttatag atgtcaataa accggctgtc gattggcatg
    14761 gaaaaaagca aagcctcaaa gaggttttga ttgatgtagt tcgttccggc gatattaaat
    14821 tggttccgga acgctttgat tccacttatt tttattgggt agaaaattta caggattggt
    14881 gtatttcaag gcaaatttgg tggggacacc gtattccggt ttattacaaa gatggagaaa
    14941 cactggccag cattcgtccg ccggaagagt tcgatggttg ggaacaagat ccggacacgc
    15001 tggatacctg gttttcgtct gcactatgga cctggagcac tttgattgat ccggaactgg
    15061 caaaaaatta ctctttgtct ctggaagatt tgttggagcg cagtccagat tttcagaaat
    15121 tccatccaac aaatatcatg gaaacaggtt atgatatatt gtttttctgg gtggcacgga
    15181 tgattttgat gacgacttat atgttgcaga atgtgccgtt taagacagtt tatctgcacg
    15241 gacttgtgcg aacccgtgat ggtaagaaaa tgtcaaaatc agttcctgaa acctgtatag
    15301 acccgttgga aagtattgct gagcacggaa cagatgcctt gaggctggcc ttgattcaag
    15361 gtaccggacc tggtcaggat ttgcggcttt atccggaaaa aatggaaagc tgccgtcgtt
    15421 ttgccaataa aatatggaac gcagggcgtt atattttgct gacaattcca cttggaacac
    15481 caaccgctcc tccggaaaaa gtacattccg agttggcaca atggcttcta catggattga
    15541 atgacttgat ccggcactca caaaccggct tggaagcaca tcgtttatca gatgttgttg
    15601 actcactgcg gagttttttc tggggtgaat tttgtgactg gtatttggaa atggataaaa
    15661 aaccggaacg taccgacgaa gacaatcaag tactggccta ttcttattcg actttgttga
    15721 agctaatgca cccctatgtt ccgtttgtca ccgaagcatt gtgggagcaa tttaaacagc
    15781 caaaaatgct ggccgtcagt gagtggccgc aagaacagtc ttattcgttc tcggaatctc
    15841 acaatcggat cgaacttctt cgtgaggcaa tatctcagat tcgtacattg cgtgaaaaag
    15901 ctaatatcgg cttggataaa aaaattggtg cgacactgga ttcagaaaaa aatgctgaat
    15961 tgttccggac acatcaaaat ttaattatcc ggcttgcacg tttaagtgaa ctggaaatca
    16021 atgaaaaaac tccggactct tcccgggaca accttggtgc atattttaat gaaacccttg
    16081 caagcatcga agcttctgcg gttgattgga aaaaagaaat cgaaagcctg caaaagaaac
    16141 tcaagactga atcaggtttt gtagaaaaaa gtcttaaaaa actcaaaaat cccggatttc
    16201 tcgacaaagc tccagaaaaa gttattattg aattgcggga aaaagttgcg tctaccgaaa
    16261 aaactctgga agccttgaaa ctgcagatcc atgaattaga taaattggtg gagtgagttg
    16321 attacaaatt cttcgtcaat tataaagtaa atcttaaaca aaagataaaa tgttaaaaac
    16381 agcagtaatt ggtgtgggtt atctgggccg ctttcatgca caaaaatatg ctgcacttgc
    16441 tgaatcagaa ctggttggtg tagttgatgt cgattctgta cagggacaaa aagttgcgga
    16501 tgaaattgga gttccggttt ttaatgattt tcatgaagta ctggatttgg ttgatgcagt
    16561 cagcatcgtt gtaccaactg tgcatcattt tgaaactgct aaagccttta tggaaaaggg
    16621 cgttcatgtt ttgctggaaa aaccctttgc ggcaaatatt gaagaagcag aagaactgga
    16681 acagctttca agggataaaa atctgcgcct gcagataggt catctggaac gcttcaatcc
    16741 ggtttttact gaatttcagg agctaattga tcggccaaaa tatattgaaa acatccgcat
    16801 tgcacctttt ccgaaacgtg gaactgatgt ggatgttatt ctagatttaa tgattcatga
    16861 cattgatttg gttttagcag tagttggtga atatccggaa tctgtcgaag gtactggagt
    16921 aaatatcatt acttccactg cagatttggc taatgcacgc ctgcgctttc cttctggctg
    16981 tattgcggat ttaacggcca gtcgagtatc cgacaaagct gaacgaaaaa tgagaatttt
    17041 tcagtcagga ctgtatctat ctctcgatta tggaacaggt caggcacgca agctacatgt
    17101 agaacccgga actgctgttg acccggagac gctccgtccg gagactttcc agttggaaaa
    17161 aggtgatgcc ctgttaacag agattgaaag ttttttgttg gcagttcgtg aagggaaaaa
    17221 tcccaaagtt actgctgaag atggattgaa tgcaatgcgg gtgggttggc aaatcaagaa
    17281 tcaactttaa aatattttat gctgcttttt cgttcaatat ttatacaatt gattgttccg
    17341 tttctgctgg cacttggaat attgactttt atcctttcaa tggaaacagt ttatcgtctg
    17401 attaacttga ttgtggaacg gggggtaagt gtttttagtg tcggattgat gctgctttac
    17461 cgcctgccgc aatttttatc tgtgactttg cccttgtcta ttgtgatttc atgcgtggtg
    17521 ttgatggtcc gtctctcaac ggacaatgaa atttctgcaa tgaattcgat gggaatcagt
    17581 tattggcaga ttgggcgtcc gttttttttg tttggaattg ttgcaacagg aattacttta
    17641 attattactc tctggcttca acctgccggt tatgccgctt ttgagcagga aaaactcagg
    17701 gtgttgaaaa cacaaactgc gaaaaccatt caaccattag tcttgaatta tgattttcct
    17761 ggaaaggttt tgtacgtaca ggacaaggat aaaaatgaag atttgagcgg tgtttttatt
    17821 accgacctga aactgactca ggactcaatg gtaacacttg cagatcgtgg acgaattgaa
    17881 attcgggaag gagagcgtga tttgctttta aacctggaag atggactaat tcatttgcaa
    17941 ggacctgctg ataattaccg tacgattgca tttgagcagt tccactatat tttccgtccg
    18001 cctcagattg ttccgtctac taaaggtggg catatctggg gtgttcccac taaagcacta
    18061 ttgcagaact caagccgctc gtcaaaaatg gaattgttct taagactcac cactccgtgg
    18121 gcttgtgtgg cttttgcggt ggcaatggtt ccattgggtc tgatcaatat acgtcagggg
    18181 cgttccggtg cttatttacg gggcttgatt ctggtggtga gttactacat tttatggatt
    18241 ggcgcaaaag aaatgacgat gaacatgaac tatcagcctt atgttttgtg gatgcctcca
    18301 cttttgattt ggctctttgg gctttatggc ttgaataaaa gcaacctcaa tctgcaaaat
    18361 atcttcgaca ttctgagttc atttggcaaa acgggttcaa gcgttaagta acatgcactc
    18421 gaaatctgag gtatttaaca agttttttgt atgaaatatt ttacatttgt atttatctca
    18481 atattattgt ttggcttttc aggacaatct gtttccaaag aaacagatct aattcaaatg
    18541 attcaggaac aatatcagag tattcaaagt ttttctggac attttgtcca aaccagttat
    18601 cggaacaata ctgagactgt cagaagagca gagggcttgg tttcttacaa acgtccaggc
    18661 aaaatgcgtt ggctttatga agttcctgaa gagcaattgt tggttactaa tggtgaaact
    18721 ttgtggctct tcgatccatt gttggaaaat gtgactatac aaaaactgga aaagctcact
    18781 gatggaacgg cactttcttt tttacttggt ttgggagact tacaggcaga ttttaacagg
    18841 cggttaatca gtcaggtttt gcttactagt ccggatgctt taattgttga actggaacca
    18901 aaaaaagcag ctgcaaattt atcatttatc cagttagcgg tgcatccggt aacatataat
    18961 ttgcagataa tagcactgat ggatcaggag ggtaattacc ggacaatcga actggagtca
    19021 atgcactaca atcttgtgct tgaagataat ttttttgaat tcaaagtcac tcaggatatg
    19081 gaagtgattg aagcaggaaa ttagcacctc cttcaataat tctgtttttt gcacaagtct
    19141 gtaaaagaac aacaagtgaa gtaaaaacct gtgattgtct aattctactg gtaatagttg
    19201 caaccaaaat tccaggatag catgcttaaa aaagaactca ctttacttaa tgtttacgcc
    19261 attgccacag gcacaacttt gagtgcaggg ttttttctgc tccctggaat tgccttcaat
    19321 gaggccggtc cggcagtagt cttgagttat atgattgcag caattccact gattcctgca
    19381 atgttcagta tggtcgagtt atccactgcc atgcccagag caggaggagc atattatttc
    19441 ctcgacagaa gcatggggcc gtttgtcggt acaatcggag ggttgggaac gtggttggca
    19501 ctggttttca aaacagcatt tgccttgatt ggaatggggg cttatctcag tattttctgg
    19561 ccggaagttc ccatcgttat gctggcggtg attttagcgg tcctttttgg aattgtaaat
    19621 ctttttggag caaaaaaaac cgggacgttt caggtattca tggtctttgc cctgctcttg
    19681 attttattag ctttcattgg aaaaggtatt ccggaaataa attcagtaca ttttgaagga
    19741 ttttttgaca aaggtactgt atcaatcatt tcaactgcag gacttgtgta tatcagctat
    19801 gttggagtaa caaacattgc cagtgttgcg gaagaaattc aaaatccaga acgaaatctt
    19861 ccacttggag tttttcttgc tatcggaact gcaattatag tttacgctgt tggtacaacg
    19921 atcatggtcg gagtgcttcc tgcaaatgaa ttggctcagg atttaacacc ggttgcttct
    19981 actgcagaaa ttatttttgg agagtggggt aaaattgctg tgactgtggc agcagttatt
    20041 gcttttgcat ctgtggcaaa tgcagggatt ttaagtgctt ctcgatatcc tctggccatg
    20101 agccgggatc atcttattcc gagtaaatta gctaaactaa ctgcacgtaa tattcctcac
    20161 tatggaatag cagtaactgt tgtattgatt atcctgctga ttcttactct tgatattgcc
    20221 cgcatcgcca agttggccag tgcttttcaa ttattgatgt ttgctttgat ttgtctggca
    20281 gtagttgtga tgcgtgaaag ccaaatagaa gcctatgatc ctggatatcg ttccccgttt
    20341 tatccgtgga tgcaaatatt cggagttttt tctccactgt ggctgattgc tgaaatgggc
    20401 gctcttccca tattattttc tctgggacta gtaacacttg gtacagtctg gtattttgct
    20461 tatgcacaaa atcgggtggt tcgttcaggt gcaatttatc atttatttgc ccgtcttggt
    20521 gaacaaagat ttgaagggct ggatcgtgag ttgcgcggaa ttatgaaaga aaaaggtgtg
    20581 cgggaggagg atccctttga cgaagtagtg gctcgtgcac aagtgatgga atttaccaag
    20641 gaacatcaat ttggtgagat tacacgtcag gttgccattg aatttgaacg caatctgggt
    20701 gtagatgcaa aagaattaga gcaaggattt ttgaagggca gcaggattgg cgcaacccca
    20761 atttcacgtg gtgcggcttt gcctcacatt cgtctgccgg atctcaaact tgcagaaatg
    20821 gtgatcgtcc gtgtaaatga attatgcttt gtagaggcac ctgatttttc gggtaaaata
    20881 tcacttcagg gacctataca tgcttttttc tatttaatca gtcccaatga aaatcctggt
    20941 cagcatctgc ggattctggc tcaaattgca ggaaggattg atgatgaaaa ttttctcaaa
    21001 gattggctgg atgcttcaga tgaacaagaa ttaaaggaaa tattattacg agatgaacgc
    21061 tttttatcac taaggattcg caacaataca ccttcttcag tattcattgg gcgttcattg
    21121 aaagatatac gtatgccaga aggctgcctg gtagccctta ttcgcagaag aggagaaacg
    21181 attgtaccgc gtggactgac ggttttactg gaaggtgacc ggctgactat tattggtgat
    21241 gcacaaggga ttgaacaatt aaacaataaa tataattaaa ttcatgaaaa caaccttaag
    21301 aactgacgga cgaaaaaaca acgaagaacg tccgcttgaa atctcacttg attatacaat
    21361 gcacgcagaa ggctcggtgc ttgtttcctc cggaaatacg aaaataattt gcacagcatc
    21421 tgttgaaaat aaagttccgg attggctttt ggataaagac aaaaagccgc atcacggctg
    21481 ggttacttct gaatacggaa tgcttccgcg ttcaaccgga agccgcagga agagagaaac
    21541 cggaaaaata gacggacgga gccaggaaat tcagcggtta attggtcgtt cgttgcgttc
    21601 cgtggtaaat ctgtcagtac ttggagaaca cactatctgg attgactgtg acgtgatcca
    21661 agctgatgga ggtactcgaa ctgcagcaat tacgggtgga tttgtggctc taattttggc
    21721 tttaagaaat atctttaaaa caggaaacat caaagaattt cctgtaaaag aataccttgc
    21781 tgccatcagt gtaggtatag taaatagcca accgatgctg gatttaaaat atgatgaaga
    21841 caaagctgca gaagtagata tgaacgttgt tatgctggag tccggagaat ttgttgaagt
    21901 ccaggcaact gcagaaggaa aaacatattc tcgtgaacaa ttggatttaa tgcttgatct
    21961 ggccgtacag ggaattcatt cacacattgc tgcacaaaag aaggtgcttg ggaatgtact
    22021 gtacggctaa cttttattcc tgaaataatt atgtatccat atattttttc tcttgaaatt
    22081 ccatggctta acattaccct tgaacctaga ttttacggat tgttttatgc agttgcggtt
    22141 ttaatcggtt cacaaattgt aattgctgaa attgcaaggc ggcgtatcaa gcttgacgag
    22201 gatgaagcaa tgaacatgac aatgctcatt tttttggcag gcttaattgg tggacgaagt
    22261 tatgaagtaa tctttgagtg gtcgaactat tatagatttc aaccattctg gaaagtcttt
    22321 gcaatttggg aaggtggatt ggcgattcat ggggggattc tcggcggaat cgtttcgctc
    22381 tatttttact gtcgaatcaa aaatatttca ttagccgcca ttctggacat aggtgcatta
    22441 tgcatgattc ttggtcaagc tcttggtcga tggggtaact tcaccaatgg agaaactgca
    22501 gggccagtca ctgaattttg gactggaata gtatttcccg caggaacagc cgtagaccgt
    22561 tatgctcatg gtgcaccagt acatccgaca atgatatatg aatcactggg caactttcta
    22621 attctagctc tactctggaa actccggctc cgaaattttc gtccaggaat gcttggcgcg
    22681 ctttatttac tctcttactc cgctttacgt tccttattga cacctctacg tatggacaat
    22741 caatattttg tcgttgctga tactaaaatt cttgcagctt atgctatcag tattttactg
    22801 gtcggtgcgg gcctgttttg gatatatcag caaaaacttt ggcttccgga tgaagcactg
    22861 agtgaaaaat catgaatgtt gatttagtgg aattattgac tcatcctgcc gttatacttg
    22921 cctcgatcat cggacttgat ttgctttttg gagatccggt ttatagctgg catcctgtac
    22981 gcctgatcgg taatttgata tcatggtttg aaaccaaact tcggtcaatc ggcctggatg
    23041 gaaaatttgg cggaattatt ttggtgctgc ttttactgtt ttgtgcattt gtagtctgca
    23101 caggcatttc acatttttta acaagaatac actggagctt aagttggctc tggtccgttt
    23161 atcttgggtg gagttttctg gctttgcgag atttgctggt tcatgcaaaa catgttgctg
    23221 aagccatgga aacagaaaat ctttctgaag caaaacggca agttggaatg ctcgttggcc
    23281 gggacaccaa actgatggat ttaccggctt gcggacgtgc cactgtagaa agtgtcagcg
    23341 agaatttgaa tgacggtgtg atggcacctt tactcttttt ttgttttttt ggaataccgg
    23401 gaatgttgtt atttaaagtt gtcagcacac tggattcaat ggttggttat caaaataagc
    23461 gctatcagga ttttggctgg tgcagtgcaa aatttgatga tctgttgagc tggattcctg
    23521 caaggatcag ttggcttttc ttgtctggaa ctgcagcatt gttcccggaa ttttccggca
    23581 gtaatgcttt caaagttggt tggaaggatc atgcaaaact tcccagctct aatgccggct
    23641 ggtgtgaggc cactgcagca ggagctttga aaattagact ttgcggtcca atctggcgtg
    23701 aaggtaaatt ggcccacaat tattggcttg gcagccagaa agacagagaa ggcgcaactg
    23761 catgggacat acaacttgtt agtagactgg ctttggcaac cactttaatt gtttccggaa
    23821 tcctgggcta tttgttatgg ttgagcgaat tcaaaccatt tttcagccca tgaaaatttt
    23881 attcacaggg ggcgtaaaaa gtggaaagtc ccgtcatgca gaaaaacgtg ttctggaaat
    23941 gtccagaggt aaaaaaccag tttatctggc aacaacagaa ttgcttgatc cggaaatgaa
    24001 ggaacgtatt tcggagcatc gacgccggcg gggggcaaag ttccacacac ttgaagagcc
    24061 actgcattta gcagatacat tactaagtac agaagttcct gtgcttgttg aatgtttgac
    24121 gatgtggctc aacaatgcca tgcattacca actctcagaa aaaaaaatct ttgccgaaat
    24181 tgaaaacttg ctgctgctcg aatgtgatgt aatatttgtg ctgaatgaag ttggaatggg
    24241 gattattcca gataattcat tagccaggaa atttgcagat ttgtccggac ggattgcaca
    24301 gcagcttggt gaggcctgcg atgaggtaaa cttgtgtgta gcaggaatct tattaaagat
    24361 aaaataaatg aggaaaactt aatgaagact cttcatgtaa atttgcctgg acgagaatac
    24421 aagattgata taggtctaaa tattcttgat cagttactac cggaagcagt gttgagtaac
    24481 tcaacagatc atgtggttgt ggttacgaac acaactctgc atgacttgta tccggatcgg
    24541 atttctcagg ttttacaaaa ttccggagta cgagtcagta cgtgtgtttt agcagatggt
    24601 gaggagtata aaaatcttga aacactgtca ctgatttttg attttttaat gaagttcagt
    24661 gcaaatcgaa aaactttact ggttgctttt gggggaggag taattggtga catggctgga
    24721 tttgctgctg caacttttat gcgcggcatt ccgttcattc aagttcccac aacattgctg
    24781 gcagacgtgg acagcagcgt tggcgggaaa accgctgtta atcatccatc agctaaaaac
    24841 acaattggat catttaaaca acctgagtac gtttgcatag aactgtcttt tttgaaaaca
    24901 ttgccctccc gtgaattgaa ggcaggtttt atagaactgc tcaagcatgg attaattcat
    24961 gacgtaaatt tgtttgaatt tattcaggga cagactctgg aaccgctgga ctttgaattt
    25021 ttggaggaag caattttcag gtcatgtgga attaaaggcc gggttgttga acaagatgaa
    25081 acagaaacgg ggttgcgtgc aatactcaat tttggacaca ctttaggaca tttgattgaa
    25141 acgcatgctg gttatggtac atacctgcat ggagaagcgg ttggtgcagg tatgtgtttt
    25201 gctgcatttg tttcatggca ttgtaatgag ctttctgaaa aagactggga gcgcatcagc
    25261 agttatttgc ggaaaatgtt ggctccggtt gttctccaca gtctggatca ggatgtcttt
    25321 cgtgatttga ttttgcatga taaaaaagca caaaagcagg cggtaaattt tattatgcta
    25381 aaaaaattgg gtgaaagttt tattcaacag gaaatgccgg tggaaaagtt gtgggatgag
    25441 ttcaagaaat tcacagcact gcatccagaa tttgtggaat tgagatgaat aatattcaga
    25501 ccattccggt ttgtgaaaaa gttgtggtta gtgggtcaaa cccaagatta tccattgttc
    25561 tttcccacct cagttcgtaa tccagatcga gcagcaaaag ttcatccagt gggccgagcc
    25621 accaggattc ctcctggatt tcccgatcaa gttgtcctgc accccagccg gcatagccgc
    25681 tgccaaagtg ataagattgt acatcttgcc cattagcaag cgaatacaga acatcttgtg
    25741 cagaacttaa gttgattttt ggtgaaagaa tggtcgtttc ttctactgaa aaatcggaac
    25801 tgtgaacagc ccaaaaagat tctggttgaa ctggacctcc gagcagcaaa ggaatatcgg
    25861 cagcaacatt ctccttcatt tcgtctgaga gaatctcttt aacgagagtt gttgttggat
    25921 gattcatgac gagtccgaat gcaccttctt cgtcatatct gcacaaaaga ataacagatt
    25981 tgttaaaata tgtatctttt agtgccggca tggcaaccag gatgcctggg acttcactga
    26041 gctgcatata gttttttgtt tgatttaaaa ttaaatgatt atgcctagtt tacctcaaca
    26101 gaaaaaagat cacaatttgg taacacaatt tctccgctca attcattata aaagctcatc
    26161 cggacaaaaa ccatacggaa atgctctgtc tatattgcgc gcaatttttg aagcatcggg
    26221 tcaacagcat ttgctttatt tagtcaaact gcgcaaactc tggtttgctg agatcgacag
    26281 ttttatggcc aggaactctt atccacgaaa tatttctgta cttcgccaat tcacagtcaa
    26341 tgatgatttt tgtcaagaat tggccaaagc aagtgtttct cctgattttt tgcaggcagt
    26401 agaaaagtta aacggagaaa gcttcaaacg ttcagaacaa ttaaatggtc agttgcaaaa
    26461 aagactgaaa cgtttgttgt ctccagaaga atcagaattg ttgaaacata aagtcaggtt
    26521 taaacatctg caaacaattt tgcacttaac cgtgtatgac ggaagcatcg ctcaggctct
    26581 ccgatttgaa actgaggctt atcttcgcat ctttcatcga ttgttgccag aattaaaatt
    26641 agatagtata aattgccatg ttggagattt ggggcaggca caatatgatc agcgtcaggt
    26701 tgcgttgctg gcaaaagact ggaatcaagt cacaccaccg gaggtgcatg aacgctgtat
    26761 gccggctttc ctacatcgtg tttcacgggg acatgcagtg ttggtgctct atgtctcaac
    26821 tgaggaaggt ttggaatatt tgaaacgcac tcctggatct gattggctgg tccagcattt
    26881 acacaaaaaa cggacagagc tgcaggaagt tttgcagaga atcgcatttg taccaaaacc
    26941 tgaactggat ttggagcaaa ttcgtttgcg ttctgttgta ctgggagaaa ttgattcgga
    27001 aaaactttct tctgttacag aaataacaag caggaagtcg actgccagta cctcaaaaac
    27061 gactctttcg gcaaaagaac agtttgctaa aattagaatg catttaaatg aaggcaccag
    27121 cgatgcagtt aattctgctt aaagagactc aaaaagcgta cagtaacttt tgtagcgttg
    27181 ctccgtaatt tttttagagg cgactgcatc tttaattaaa cattgtggct cgtgaatgtg
    27241 cgtgcagcct ttgaatttac actgccccaa atatggaaca aattctacat aatgagaatc
    27301 cagctcattc ggtgtcacca gtggttccag ttcacggatt ccgggagtat ccactataaa
    27361 gcctcctata ggaatacgaa acatttctgc cattctggta gtgtgacggc ctttaccaga
    27421 tttgttgctt atacgaccga tgcggatttc ccagtcagga aaaagagcct ttatgagcga
    27481 tgattttcct actccagaat gtccggaaag gacggttgtg cgattttgca gtaaatcccg
    27541 cagcgactcg agacctctgg attccggaat acttgtgact aaaatggtat agccgaggga
    27601 agaatagagg ttttcaatcg gagatatttc atctgaagtt gccaaatcag ccttgctcag
    27661 gatgagcaca ggctcgagtc cgccacatga agcagccact aacaagcggt ctaccaatcc
    27721 tcctcggaac ggtggggtac gcatcgaggc aaccatcaga agtgtatctg cattgctgac
    27781 taaaacctgt tccggatacc cgacactcgg gcgacgcgaa agtttggttc gtctgggaaa
    27841 catccggagg atccaccccg catcttccgc tgctgtcaga tcgacttcga cctcatcgcc
    27901 aactgcaatt ttatttatgt aggagctttc tttgaacaag tttccacgga tgcgacatag
    27961 gcggggctct atttctccaa catccacata gtaaaaactt ttggtgtgcc tgacgatggt
    28021 tcctgtttgc gtcaatttcc tccggcaagc gtgtgggtca ttcttccggt tcgagctcga
    28081 aatctgttcc ttctgctcga tattcgttga gcttatttcg cagagtccga atactgatac
    28141 ctaaagtttt tgcagcatga gtacggttac cttttgtctc atggagagtt ttgaaaataa
    28201 aacgcttttc tgcttccttc attgacattc ccacttctat tcccagacca ttatctggtg
    28261 agttttgtgg ttctgaaaaa gtaaacgatg tctgagagga atatgatgct ggagtttttg
    28321 aatagtctgt cgttattgct gccaaatctt catttatggt agtctcccca agttgagaag
    28381 acatgagtaa atgactgggt tggatttcct caccgttaca gagcagcaaa gcacgctcca
    28441 tgacgttttc taattctcga acattaccac gccaatggta atttgtgaga atatttaagg
    28501 tttcacgact tactgcggga caattacgtt tgtttatctg ggagtgcttt ttgataaaat
    28561 atgttaccag taccggaaca tcgtccatcc tgtcccgcag aggaggaagc gcaagaggaa
    28621 ttacgtttaa acgaaaataa aggtcctcgc ggaattttcc agaatcaatc atttccagca
    28681 aattctggtt ggtagtggca atcactctga catccaccgg aataggttct ttaccgccga
    28741 ctttgtcaat ttgatattcc tgcagtacac gtaagagttt tgcctgcaga ggcaaagcca
    28801 tttcactgat ctcatccaaa agaattgtac ctgtgtgggc ttgttcaaat tttccgcgat
    28861 aatcctgaat agctccagta aaggcacctt ttgcgtgtcc gaagagctca ctttccagca
    28921 atgtctccgg aagtgctgca cagtttattg caacaaaagg tctcttcgca cggtcagaat
    28981 tttcatggat ataatatgcc agcagttctt tacctgttcc gctttcactc tgaatcatca
    29041 ttgttgattt actttttgct acattcttgg caacttttag taaatccaac attgtttggt
    29101 tctgcgtgat gatttctttg gtcacattta tcggttttga ctttgaagtc ttgctttcag
    29161 ctactggagc tgcagttcca ggagtgcctt tgtaattcaa ggcttgctgg acaataaact
    29221 gaaatgaatc aaaattaatt ggtttgcgga tgtaatcgaa agcaccgtgc ttcattgctt
    29281 ccactgctgt ttctacagtt ccgtaagcag ttgccataat cacgggcttg gcaggtgaga
    29341 tgctttttat gtctttaagt aattccagtc cactgcgttt tggcatggtc atatctgtaa
    29401 tgaccagaga atattcgttt tgcttgaact ttttgagtgc atcaattgcg ttgtgcgata
    29461 tttctaccgg gtagccgcag tgtttcagtg tttcagacat ggcgatacgc atttcaactt
    29521 catcgtctac aatcaaaacc ggttggttga ccatattaaa ttcctttcat ttgatgtcca
    29581 tcaggcggtc atcttatatg cattcagtgt taaagtttta aaacaggata gtatattagt
    29641 ttggggcagt cgatttagct gacattttgg catgatcata acatatccgg ttggttaatg
    29701 cacgattcat gcgaagtcaa atagtctttt gtttcaaaga tgtaaactgc actatctgaa
    29761 aattaaattt cttgttcaac aacatccacg cttattagtc atgacatagt ataaacagtg
    29821 tttttgagtt gaatattcca agcactctgt tagtgtttat gttatgtttt aataaaataa
    29881 aaaggagata aaatgagcct tgaagaagat ttcaattctg cagctgaacg tgcgacccag
    29941 cttccaaagc gtccaccaaa tgatattatg ctccaacttt atgcacttta taaacaagct
    30001 aacgaaggcg atgtaacagg tgaccgtcca ggctttgccg attttgaagg tcgggtaaag
    30061 tttgatagct ggaataaact ccaggggaaa tctggcgaag atgcgaagca ggaatatatt
    30121 tctcttgtcg aaaaactaga acaagaagtt gccgaataaa aacttcatgc cattcgatct
    30181 ttccttaata gatctagttg aacgcatcaa gtcaggagaa ctccgtgcag aggcagtgat
    30241 ggaggcagtt tattcccgga ttaatcagta caactcttcg ttgaatgtct accgtagtct
    30301 tttgccgcgt gaaattgcaa tttctcgtgc acgtataata gacgaggaaa tttcttccgg
    30361 aaagaccctt ggcaaacttg caggtattcc ggtatcgatt aaagacaata tctgtatcaa
    30421 agatccggag ttgagcaatg catgtggttc acaaattctg ggtgaatatc actctccata
    30481 caatgcaact gtagtagaga aattattgca ggaagatgca gttattattg gaactacgaa
    30541 tatggacgag ttcgcaatgg ggtcgtccac agaaaattct tttttcagtc cgacccgtaa
    30601 tccgtgggat acggaacgtg ttccgggtgg aagtagtggc ggtgcggcgg tttctgttgc
    30661 ttccggaatg gccttgttgg ctctcggttc tgataccgga ggttcggtgc gccaaccggc
    30721 ttcgatgtgc ggtgtttccg gattaaagcc atcttacggc actgtttcgc gttacggcct
    30781 ggtggctttt ggttcgtctc tagatcaaat cggttgcctt gcacccagtg ctgcggattg
    30841 ccgttatgcc ttttcaatca ttcacggaca cgatccgcac gaaagtactt ctgcaaaaat
    30901 agaattatca actgctccag atgttgcaaa actctccggt ttgcgggttt gtcttccgga
    30961 tgaatatgta gctgcagaat cttttgatcc ggatgttgta gggagcgttc atgagttaga
    31021 ggcactgttg cagaaacacg gtgcaacggt tgaacggcgt tcactggatt ttctgaaata
    31081 tgttattcca acttattacg tcctcgcttt tgcagaagcc agttccaacc tgggacgttt
    31141 cgacggaatt cgttacggaa taaggaagca ggatcagcca tccttgaaat cactatacac
    31201 atctaccagg aaaaagggtt ttggtgtgga agtaaaacgc agaatcatgc ttggaacatt
    31261 tgtactcagt tcaggttatt atgatcaata ttatggacga gctgtgcagg tgagggcttg
    31321 gatggaaaaa caagtggcat tgctgtttga ggaatttgat tttatactgg gaccagtttc
    31381 tccttttcct gcattcaagt taggagaaaa aaatgaagat ccactggcaa tgtacttggc
    31441 agatatttgc agtgtacttg caaacctgac acgaacacct gcaatctcaa ttcctggaag
    31501 accaaccagt gacggattac cagttgggtt acaattaatg ggccggcgtt ttgaagatca
    31561 taacctttta gcaaccgcat ctgccattca agaactgact gcatttcata aactgcgacc
    31621 agaaaaagtt tttgcaacaa aaccataaaa catgaaaagt ggattgcaaa tctgcaatca
    31681 aagaatctta aatctcaaac ctgaaacctg actgaatgct tgatctaaaa ataatcagaa
    31741 gccagcctga agtaattgca gagaattgcc gaaaacggaa cgttgacgta gatattgaaa
    31801 aattgttagc acttgatgag caagtaaggc agattacatc tgaagttgat tctgtcagac
    31861 aacgacgtaa tgagatcagt aataaaatga aagtcaaaat tccacctgag gaacgtcagt
    31921 ctctgattga agaatcaaaa aacttgcgtg aagaagagtc ggaaaaagat tcaatacttc
    31981 gagaactgct tgagcagcgg cttgatttgc acaagcaggt tccaaacttg acacatccag
    32041 aatctccgga aggttttact gatgaagaaa atgttccaat tcgggaagtt ggaacaaaac
    32101 gcgaatttga ttttgaacct aaggaccatg ttgagttaat ggaagcactt gatttgattg
    32161 actttgacgg aggtgcaaaa gtttctggac aaaagtttta ctacctcaaa aatcaggctg
    32221 tatttttgga attagctttg gcccaatatg ccctgagttt gctgcaaaaa gaaggattta
    32281 cagtacatat tacaccggac ttggctcggc atcagatttt ggacggtatt ggatttaatc
    32341 cacgtggtgc agaaacacag atttattcag tagaagaatc ggatctctgt ctgattgcga
    32401 ctgcagaaat aactttgggc ggtttgttga tcgatcaggt actccaaccg gaacagttac
    32461 cgattctgta tgcagggctt tctcattgtt accgcacaga agcaggtgcg gccggacgtg
    32521 aatcacgagg attatacagg gtacatcagt tttctaaaat agaaatgttt gcctttaccc
    32581 atccagataa gtctgaagaa atgcatgaaa aaatgcgtga aattgaggaa caaatttacc
    32641 aggatttgga gatcccatat cagctggtga atatttgcgc tggtgacctt ggaggaccag
    32701 cttttcgcaa gtatgatttg gaagcctgga tgccggggcg tggcaaatgg ggcgaggtga
    32761 catccacttc aaactgtaca gattatcagt cacgtcgcat gaacattcgc tttaaagacc
    32821 cggaaacagg aaaaaacaga tttgtccata tgctaaatgg aactgcaatt gcggtttcca
    32881 gaactttgat tgccctcata gagaatggtc aacaaaaaga cggttcaatt aaactcccgc
    32941 gttgtttgaa tattaaggat attccagcac ctgcttccgg ataatagtat ttttgtcttt
    33001 caattgagtt gttttagttt taatgtatgc attcttaata accctttgcc caaaaaaata
    33061 atatgaaaat actcgcaatt ttaacaattt ttattatttc ttcaactttt gtttttgggg
    33121 aattgcccat tggtgaagtt ccgccaacaa ttgtacttga aggtgattta ggaggaagac
    33181 ttgatggtac tcagtggagc agtgaggaat tggtttccgg aaaagtcctt gtcctttttt
    33241 atgtcgatcc ggacgaatct gatctgaaca accatgtaag tgaagctctc aaggcagaaa
    33301 aataccctcc agaaaattat ggttctgttg cagtcatcaa catggcagca acatggcttc
    33361 caaattttgc gattaacatc aaactgaaaa gcaaacaaga ggaatataaa acaacagtct
    33421 atgtaaaaga ccttgaaaaa actcttgttc aaaagtgggg gttaagtgat gataacagtg
    33481 acgtagtgct cttctctaaa gatgggaaag tactttatag tgttgatggt aaattcactg
    33541 atacgcaggt taaggaaata gtaaaaaccg tttgggataa tttgtagtag tggaagactc
    33601 aaaattcggt tgattattaa tcagtttgac ttcgcaaatt tctttcgggc ctgaacagca
    33661 agttcgtcgc agcggtcatt ttcgggattc ccggaatgac ctttgaccca atgccatgaa
    33721 acttcatgct tcttactgac ttcgagcagg cgaagccaga ggtctttatt tgcaacaggt
    33781 tttcttgctt tacttttcca gccgttattc tgccaactat ttaaccagtt tttctcaaat
    33841 gcatttttta gataactgga atctgtgtag agtttaacaa cacatgattt ttttaaggct
    33901 tccagggctt tgatggcagc agtcatttcc atacggttat tagtagagtc aacttcagag
    33961 ccggaaagtt cggtgcggag attgtgttct ttggaaatca aaactgctcc ccaacctcca
    34021 attcccggat taggcgagca ggcaccatcg gtgtaaattg taacagatgt ttttagcatt
    34081 tttttgtgaa tttgtctttt gaagaatcaa agtataacag aaagaacatc tgactgcgat
    34141 ttcaaactgc atttttttag taatttctgc ttgttgagtt tgtggggcag atgaatattg
    34201 attttaaaca atttggaaca tggatctatg ttgatccgga tgaacagagt atagagcttt
    34261 tagaagagac tcttaattta gacatgcttt gtgaattatt acgttgtgaa tctactgata
    34321 tggaagagtt gggcggacca ttcctggctt attttgatgg agaaggagca tcgcaggaaa
    34381 gacagacaga atgggaattt aatgaaaatc catgttgggg tccaatactg attgtcaaaa
    34441 tcggaaaaga agattctagg cttgaaacct gtgatgagga tgatctggca gtaatagagc
    34501 cactggtaaa atttttagac tgacaaaaaa aggaataatt agatatgaat gtttcaactt
    34561 ttcgcgcatt ggtggtcagt aaaactgacg aaaaaacttt tacccgtgaa atcaccgagc
    34621 gctcaatcag cgatcttccg gaaggtgagg tgctcatccg ggtacatttt tcttcactga
    34681 attataaaga cgggctttca tgcattggaa atccaggtgt aactcgcaat tatcctcaca
    34741 cgcctggaat agacgcatcc ggaaaggtta cagaaagttc agattcgcag ttcaagaccg
    34801 gtgattctgt gattgtcacc agttatgatt taggtatgaa tacctccgga ggttttggag
    34861 aatacatacg cgttcctgct gattgggtgg ttccacttcc ggaaggtctt tctttcaaag
    34921 atgcaatggt ttatggtaca gcaggtttca cggctgctct ttcggttcat gcactgcaaa
    34981 aacacggagt gtctccggag cagggtgaaa tcgtagtgac cggttcaaca ggaggtgtcg
    35041 ggagcatatc agtagctcta ttgtcacatt tgggatacaa tgttgtagca gcaacaggaa
    35101 aaacaaatga aacagaatat ttgagcggat taggtgcaaa agaaatcatt catcgagatg
    35161 aagtaaaaga tgattcaaat aaacctttgt tgagagaacg ctgggctgga gcaatagata
    35221 cagttggagg tactacttta gctacgttgc ttaaagcgac aaaacgcgga ggggcagttg
    35281 cgtccacagg gttggttgat tcagctgatt taccgacatc agtttttccg tttattttac
    35341 gcggagtgag tttgttaggt attgattccg gatttacccc gacgaaactg cgtcgggaaa
    35401 tttggaacaa acttgcaggc gactggaaat tatcacaact gggtcagttg aaaattgact
    35461 gtaatctgga gggactggat ccggaaattg acaagatcct tgctggtggt cagcgtggac
    35521 gtgtggtggt ggatctgcag tagattgact gccgtaattt taatacaaat caggagataa
    35581 tcatgagtga tacgattaaa tatttgcttc ccgaaactga aataccaaaa gactggtaca
    35641 atattgttgc agatcttccg gagccgcctc cgccagttct gcatccaggt acacatgaac
    35701 cggtaggtcc ggatgacctt gccccactgt tcccgatgtc cttgattatg caggaagtgt
    35761 ctggcgaacg ctatcttgaa attccgggac cggtacgtga
//
LOCUS       DTVD01000030            3015 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13037_length_3015_cov_265.494257, whole genome
            shotgun sequence.
ACCESSION   DTVD01000030 DTVD01000000
VERSION     DTVD01000030.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13037_length_3015_cov_265.494257"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="EYO68_01150"
     CDS             complement(<1..403)
                     /locus_tag="EYO68_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIC06443.1"
                     /translation="MKKLAKMMAVAIASVVLSAGSLSAVEIKKIHFLIPGGAGGGWDG
                     TARGTGEALTKAGLIDSATFENMSGGGGGKAIGYLIENSKSNHGTLMVNSTPIVIRSL
                     TKVFPQNFRDLTLIAGTIGDYAALVVPAGSSY"
     gene            703..2325
                     /locus_tag="EYO68_01155"
     CDS             703..2325
                     /locus_tag="EYO68_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIC06444.1"
                     /translation="MYSFNTVSLPNFLLPRTLHGRMLLLMFLLLGALVSITWLMVSML
                     VSSILEEYIGRNALNVSKTVSLTTVVHEGLKNKNSTQIQLYAESVRKATGARFVVVGD
                     HEGRRYSHPVPERIGKLMVGGDNPRALERGEAYVSKAVGTLGPSMRGKVPIFANSGKV
                     IGVVSVGYLQETVESVTEGYLQRVLLWVFGLFLLGGIGTWLIARNVKQSIFGLEPVEI
                     ARLFRERNAILDSIREGVVAINDKGQVTMLDHEAAKILKIPPESGIGTSIESILPQTR
                     MLEVLKSGEEQFDQEMIIGGIEVIVNRVPIWQNGRVAGVVSSFRRKDEIDRMAQELTQ
                     IQEYSEVLRTQTHEYSNKLHTLAGLIQLGSHQEALDLIGRETSGYQELLGTLAETVPE
                     PLLSAIILGKYNRAQELRIIFQLDPESRMIDIPKKINREKIVTILGNLLENALEAAQE
                     NTSGKRTVLLSMTDFGNDLIFEVEDSGSGIEDESVDLSLQHGFTTKSGTGRGIGLSLV
                     HENLKYLGGHLTVVRSSLGGMRFTIYIPKHSN"
     gene            2340..>3015
                     /locus_tag="EYO68_01160"
     CDS             2340..>3015
                     /locus_tag="EYO68_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC06445.1"
                     /translation="MNLRVQIIDDDVRIAEIHRQYVEKTEGFEVVGIANTLEDAQSQL
                     DVLEPDLLLLDIFFPEGNGLDFLKKARLHNKQVDVIPITAAKEVHLFNEALHGGVFDY
                     ILKPVVFSRFQATLERYRQTKNQLSSIDQLDQSEVDRALHTVRSGDLHELTELPKGID
                     RLTLEKVQQVFGSSQDSRLTSEEVASHVGASRTTVRRYLEYLVSQIILDVDVSYGGVG
                     RPERRYF"
BASE COUNT          865 a          614 c          695 g          841 t
ORIGIN      
        1 tgtatgaact accggctgga actacaagtg cagcatagtc accaattgtt ccggcgatca
       61 aggtcagatc ccggaagttc tgcggaaaga cctttgtcaa tgaacggatt acgattggtg
      121 ttgaattcac catcagcgta ccgtgattgc ttttggagtt ttcaattaga tagccgattg
      181 cctttcctcc accgccaccg gacatatttt caaaagtggc actgtcaatc agtcctgctt
      241 tagtcagggc ttctccggtt ccgcgtgcgg ttccgtccca tcctcctcct gcaccaccgg
      301 ggataagaaa atgtattttt ttaatttcca ctgcagaaag actgcctgcg gaaagaacaa
      361 cagatgcaat tgcaactgcc atcattttag cgagtttctt cattttgttc tcctatatgg
      421 acaattaata aaaccggctt gaggccggcg gtttagaaaa accagaaacg aaatgcttcc
      481 ggtcagccac gctcttcaca tagaagattt gataccgcag ctctttagtg tcaaacataa
      541 ttgcagatac aatataaatc tgcaagagtg cattaacggg aagctaatgc taaactaata
      601 aaaagatact aacaagatta cgcagttaaa aaattttctt tttgttttaa tcataatgtt
      661 cattttgttc acaattgatt ttctcaaccc taaaaaaata tgatgtattc tttcaatact
      721 gtttcactcc cgaatttttt actgcccaga actctgcatg gacggatgct gttgctgatg
      781 ttccttttac tgggtgcatt ggtcagcatc acctggctga tggtttccat gctggtttct
      841 tcaattctcg aagaatacat tggacggaat gccttaaatg tctcaaaaac ggtttctctg
      901 actactgtag tgcatgaggg attaaaaaac aaaaactcaa cacagataca gctttatgca
      961 gaatcagtac gtaaagcgac aggtgcacga tttgttgtgg ttggagatca cgaaggtcga
     1021 cgctacagtc acccagtacc ggaacggatc ggcaagttaa tggttggcgg agataatcca
     1081 cgtgcgctgg aaagaggtga ggcctatgtt tccaaagcag ttggcactct cggtccgtcg
     1141 atgcgtggga aagtcccaat ttttgctaac tccggtaagg taataggagt agtatctgtt
     1201 ggatatttac aggaaacagt agaaagtgtt acagaaggct atctacaacg tgtattgctt
     1261 tgggtttttg gtctcttttt gttgggagga atcggtacct ggctcattgc gcgtaacgtg
     1321 aagcagtcga tttttggttt ggaaccggtt gagattgccc gcctgttcag ggaacgtaat
     1381 gctattttgg attcaatcag ggaaggtgtt gtcgccatca acgacaaagg acaagtcacc
     1441 atgctcgacc atgaagccgc caagattttg aagatcccgc ctgaatccgg aattggtaca
     1501 tcgattgagt cgattttacc ccagacacgg atgctggaag ttttaaaaag cggagaagaa
     1561 caatttgatc aggaaatgat aattgggggt atcgaggtta tagtcaatcg tgtaccgatt
     1621 tggcaaaatg gtcgagttgc cggtgtcgtt tccagtttcc ggcgaaaaga tgagattgat
     1681 agaatggctc aagaactgac tcaaattcaa gaatattctg aagttttacg gacacaaacc
     1741 catgaatatt ccaataaact gcacacactg gcagggctga ttcaacttgg ctcacaccag
     1801 gaagctcttg atttaatcgg tcgtgaaact tccggatatc aggaactgct tggtacattg
     1861 gcggaaactg ttccggaacc gttattgtct gcaataatcc tgggcaagta caaccgcgca
     1921 caggaattgc ggatcatctt ccaactcgat ccggaaagca gaatgatcga tattccaaaa
     1981 aaaataaatc gggaaaaaat agtaaccatc ctgggcaatc tgctggaaaa tgcattggaa
     2041 gcagcccagg aaaacacttc cggaaaacgt acggtactgc tttcaatgac agattttggc
     2101 aatgatctaa tttttgaggt ggaagattct ggttcaggaa ttgaagatga atcggttgac
     2161 ttatcgcttc aacacggatt tactaccaag tctggtaccg gacgcggaat tggactttca
     2221 ctggttcatg aaaatttgaa atatctggga ggtcatctga cagtagtccg cagttcactc
     2281 ggcgggatgc gctttacgat ctatattcct aaacactcta attaatttgc aggtatgaaa
     2341 tgaatctgcg tgtacaaatc attgacgacg atgttcgtat tgcggaaatt caccggcaat
     2401 atgttgaaaa aacagagggt tttgaagtgg ttggtattgc caacactttg gaagatgctc
     2461 agtctcaact tgatgtgctt gaaccggatt tgctgctgct ggacattttc tttcctgaag
     2521 gtaacggcct ggattttcta aaaaaagccc ggctgcacaa caaacaggtt gatgtaattc
     2581 ctatcactgc ggcaaaagaa gttcatttgt tcaatgaagc acttcacggt ggggtatttg
     2641 attacatcct caaacctgtc gtgttttcgc gatttcaagc aacattagag cgttaccggc
     2701 aaacaaaaaa tcaactttca agtattgatc agctggacca gtcagaagtt gatagagcac
     2761 tgcacaccgt aagaagtggt gacctgcatg agttaacgga acttcctaaa ggaattgaca
     2821 gactcacctt agaaaaggtg caacaggtat tcggttcaag ccaagattcc agattaactt
     2881 ccgaagaagt tgcttcacac gtaggagcaa gccgtaccac tgtccggcgt tatcttgaat
     2941 atttagtttc ccaaattatt ttagatgtag atgtgagtta tggaggggtg ggacgtccgg
     3001 aacgtcgata tttca
//
LOCUS       DTVD01000031            3010 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13071_length_3010_cov_258.234856, whole genome
            shotgun sequence.
ACCESSION   DTVD01000031 DTVD01000000
VERSION     DTVD01000031.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13071_length_3010_cov_258.234856"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="EYO68_01165"
     tRNA            complement(1..77)
                     /locus_tag="EYO68_01165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(41..43),aa:Met,seq:cat)
     gene            complement(108..183)
                     /locus_tag="EYO68_01170"
     tRNA            complement(108..183)
                     /locus_tag="EYO68_01170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(148..150),aa:Gly,seq:tcc)
     gene            complement(326..862)
                     /locus_tag="EYO68_01175"
     CDS             complement(326..862)
                     /locus_tag="EYO68_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIC06446.1"
                     /translation="MRGYFGVGIEQSSKPMNAGNLFRTAHAFGASFLFTVNTEYSVTK
                     AKSDTSAAPRNIPWYDFNSAEDLKLPGGCVLVGIEFLEDAVELPVFRHPPNAAYILGP
                     EMGNLSPEILQRCRHVIKIPTSFSLNVATTGAIILYDRIRTLGNYGERPVSPSGDPLP
                     PLEHVHGGPIRRKTKNKA"
     gene            complement(868..>3010)
                     /locus_tag="EYO68_01180"
     CDS             complement(868..>3010)
                     /locus_tag="EYO68_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC06447.1"
                     /translation="WKALSGHERARYLYALARQLQKHSRLFAVLETMDNGKTIRETRD
                     IDIPLVIRHFYHHSGWAQLLESEFPEHMAIGPIGQIIPWNFPLLMLSWKVAPALAAGN
                     TVVLKPAEYTPLTALLFAEICSQVGLPHGVFNLVTGPGQTGSLMVNHSDLKKIAFTGS
                     TDVGRLIREATADTDKKLTLELGGKSPFIIFEDADLDSAIEGLVDAIWFNQGQVCCAG
                     SRLLVQESVAEKVIDRLKRRMAKLRVGDPLDKSMDMGAIIAPIQKERIQNLVDQGKKE
                     GAAIWQWEGKLPQNAEKKGDGCYFPPTLFTNVSPASIIAQTEIFGPVLVSMTFRTTDE
                     AVMLANNSAYGLAASVWSENINQALHVAPKLKAGVVWINCTNQFDASVGFGGYRESGY
                     GREGGREGMFEYLKPKESGISKNQFRISVAKVKASATTALSLDRTAKLYIGGKQARPD
                     SGYSLNVVNADGSLAGEIGDGNRKDIRNAVEAAHKASGWQSSTPHLRAQILYFLAENL
                     ETRAVEFKNRITQLTGVTEKQAALEVKTAVSRIFTYAALADKHEGLIHQPPMRGLALA
                     LNEPIGVLGLVCPDEAPLLSMLSMLLPAIAMGNSVVLVPSRPFALVATDFYQVLETSD
                     VPSGVVNIVTGDSAKLGTVLAKHDDVAGLWVAGTKTECTEACKLSTGNMKIIWTNNGK
                     QLDWFDNQQSEGREWMRRASQVKNVWIPYGE"
BASE COUNT          764 a          740 c          680 g          826 t
ORIGIN      
        1 tggtggcggg ggaaggattt gaaccttcga ccttcgggtt atgagcccga cgagctacct
       61 gactgctcca ccccgctaca agaggaataa tgaaagaaat cgatttctgg agcgagagac
      121 cgggttcgaa ccggcgaccc tcagcttgga aggctgatgc tctaccaact gagctactct
      181 cgcctcatct tatcagtcat tctagatgct aaacctgcat tctgtcaata catttatttt
      241 cgcagtaatt tattttgtaa aaaaataatg gggactgtct gattaagaaa aaatcattaa
      301 tatgtaattg tgcgtcagac gccttctaag ctttgttttt agtcttacgc ctgattggtc
      361 caccatgaac atgttccagt ggcggcaagg ggtctcctga ggggcttacc ggacgctccc
      421 cataatttcc cagagtacgg atgcggtcat agaggataat cgcaccggtt gtggctacat
      481 taagactgaa actggtgggg atttttatga catgccggca gcgctgtaat atttccggac
      541 ttaaatttcc catttcgggg cccaaaatgt atgcggcatt tggtggatgc cgaaaaactg
      601 gcaattctac agcatcttca aggaactcaa tacctaccag aacacagcct ccgggaagtt
      661 ttaaatcttc tgcagaattg aaatcatacc agggaatgtt ccgaggtgct gcagaagtat
      721 cggattttgc cttggtgacg gaatattcgg tattgacagt aaacagaaag ctcgctccaa
      781 aagcatgagc cgtgcgaaac aggttccctg cattcatcgg tttgcttgat tgttcaattc
      841 ctacaccgaa atatcctcgc ataactttca ctctccatac ggaatccaga cattcttaac
      901 ttgagatgcg cgccgcatcc attcgcgtcc ttctgactgc tgattatcaa accagtcaag
      961 ttgttttccg ttattggtcc agatgatctt catgttgccg gtacttagtt tacaagcctc
     1021 cgtacactca gttttagttc ctgccaccca taatcctgct acatcatcat gtttagccag
     1081 aacggtaccg agctttgcag aatctccggt tacaatattg actactccgg aagggacatc
     1141 tgaggtttcc agaacctgat aaaaatctgt ggcaaccaaa gcaaatggac gcgacggcac
     1201 aagcacaaca gaattcccca ttgcgattgc cggtaaaagc attgacagca tactcaaaag
     1261 cggagcttca tccggacaga caagtcccag cacaccgatc ggttcattca gggctaaagc
     1321 gagtcctcgc atcggcggtt gatgaatcag tccctcgtgc ttatcagcta aagccgcata
     1381 agtaaaaatc ctgctcacgg cagtttttac ttcaagcgcg gcctgttttt cggtgacgcc
     1441 cgttagctga gtaattctgt ttttaaattc tacagcccgt gtttcaaggt tttctgccag
     1501 aaaatataga atttgtgccc gcaagtgagg agtgctgctt tgccatccgg acgctttgtg
     1561 ggcagcttct acggcattcc ggatgtcttt gcggttgccg tcgccgattt ctcctgctag
     1621 tgaaccgtca gcattgacaa cgtttagcga ataaccggaa tccggacgag cctgtttccc
     1681 tccgatgtaa agcttggcag tacgatccag tgataaagca gtagttgcag atgctttaac
     1741 ttttgctacc gaaattcgaa attgattttt cgaaattccg gattcttttg gcttgaggta
     1801 ctcaaacata ccctcacgtc ctccttcgcg gccgtatccg ctttcgcggt aaccgccgaa
     1861 tccaacagat gcgtcgaact gatttgtaca gttaatccag accacaccgg cttttagttt
     1921 cggagcgacg tgcaaagcct gattgatatt ttcgctccag acactggcgg ctaatccgta
     1981 agcactgtta ttggccagca tcactgcttc atccgtggtg cggaaagtca ttgaaaccag
     2041 caccggaccg aaaatttcag tttgggcaat aatcgatgct ggtgaaacat ttgtgaaaag
     2101 cgtaggtgga aaatagcaac cgtcaccttt tttttctgca ttttgaggta atttaccttc
     2161 ccattgccag attgcagcac cttccttttt gccttggtcg accaggttct ggatgcgttc
     2221 cttttgaatt ggggcgatga ttgctcccat gtccatgctt ttgtccagcg gatctccgac
     2281 cctcagtttt gccatacgcc tcttcaagcg atcaatcact ttttcagcaa cgctttcctg
     2341 caccagcaaa cgcgatcccg cacagcagac ctgtccctga ttaaaccaaa ttgcgtccac
     2401 cagaccttca attgcgctgt ccagatcagc atcttcaaaa ataatgaacg gagatttacc
     2461 acctagttca agtgtcagtt ttttatcagt atcagcagta gcttcacgaa ttaaccttcc
     2521 aacatcagtc gatccggtga aagcgatttt tttcaaatca gaatgattta ccattaagga
     2581 tccggtttgg ccgggaccag tcacgagatt aaaaactcca tgcggcaagc ctacttgcga
     2641 acaaatttct gcaaagagga gggcagtcag cggagtgtat tctgcgggct ttaaaactac
     2701 cgtgtttccg gcagccaaag ctggcgctac tttccatgaa agcatcagca aagggaaatt
     2761 ccagggaatt atttgaccta ttggtcctat agccatgtgt tccggaaact cactttccag
     2821 cagttgtgcc cagcctgagt gatgataaaa gtggcggatt acgagcggaa tgtcgatgtc
     2881 acgggtttcg cgaatcgttt ttccgttatc catagtttca aggacagcaa atagccgtga
     2941 gtgtttttgc aactgtctgg cgagagcata taaatagcgg gcacgctcat gtccggaaag
     3001 ggctttccac
//
LOCUS       DTVD01000032            3009 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13081_length_3009_cov_286.298917, whole genome
            shotgun sequence.
ACCESSION   DTVD01000032 DTVD01000000
VERSION     DTVD01000032.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13081_length_3009_cov_286.298917"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="EYO68_01185"
     CDS             complement(<1..853)
                     /locus_tag="EYO68_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC06448.1"
                     /translation="MTAYIIRRLLLVIPTLIGIMTVNFIIIQIAPGGPVEQMIAQLQG
                     EAVSATERLSGGGEDVKGQKSAVSDKPSKYRGAQGLAPELVEEIERMYGFDKPLHVRY
                     FKMMRDYASFNFGESFFRDQKVVDLVLEKMPVSISLGIWSTLAVYLVSIPLGIRKALK
                     HGSRFDVWSSTIVILGNAIPTFLFAILLIVLFSGGSYFSWFPLRGLVSDNFSTLPWWQ
                     QLLDYFWHLVLPITAILIGSFASLTMLTKNSFLDEINKQYVITARAKGITERRVLYKH
                     VFRNAMLI"
     gene            complement(896..2746)
                     /locus_tag="EYO68_01190"
     CDS             complement(896..2746)
                     /locus_tag="EYO68_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC06449.1"
                     /translation="MKISRLVISFIFTATFITSYSISVFAGSDLTKASSIAMNGSAKY
                     PDGFTHFDYVNPDAPKGGSVRLNSIGTYDSFNGFISKGVSADGLGMIYETLTTGSSDE
                     AFTEYGLIADEIEYPEDRTWVIYHLNPKAKFHDGEPIKVEDVIFTFNVLLEKGAPFYK
                     SYYGDIVEVKDLGDRRVKFQFREGVTNRELVLITGQLPVLPKHYWESRDFSKSTLDPP
                     LGSGAYRIKDFKAGKYITYERVKDYWAANHPVNKGSNNFDTVRYDYYRDATVSLEAFK
                     AGEYHFREENNSKMWASMYSGKTFEEKLTIRAEIPHELPRGMQGFAFNLRRSIFKDRR
                     VREAISYAFDFEWSNKNLFYGQYRRTDSFFENSELASSGLPSPEELAILEPFRKDLPE
                     EVFSKKFRPSLTDGTGNIRSQLRAASKLFKEAGWEIKEGKRVNSSTGEQLKFEILLIS
                     PAFERIVLPFKKNLERMGIEASVRVVDTSQYVNRIRSFDFDMFVMVIGQSLSPGNEQD
                     NYWSCKAAETSGTRNFIGICNPAIDKLIRLVIEAPDREKLINRTRALDRALLWGHYVV
                     PHWHINYYRLAYWNKFSRPEITPKYSLGFFTWWVDESKEKTLLAKKPNLK"
BASE COUNT          833 a          642 c          667 g          867 t
ORIGIN      
        1 ttatcagcat ggcattacgg aaaacatgtt tgtaaagcac ccgcctttcc gtaatccctt
       61 ttgcgcgggc cgtaatcacg tattgtttgt ttatttcatc gagaaaggag tttttggtga
      121 gcattgtcag ggaggcaaaa ctaccaatta aaattgcagt aatcggcagc accagatgcc
      181 agaaatagtc aagaagctgc tgccaccagg gcaatgtgga gaagttgtca gatacgagtc
      241 cgcgcagagg aaaccagctg aaatagcttc cgccggaaaa aaggacaatc agtaggatcg
      301 caaacaaaaa tgtgggaatg gcattgccga gaataacaat ggtgctgctc cagacatcaa
      361 agcgtgagcc gtgtttgaga gcttttcgaa ttccgagtgg aatgctgacc agatagacgg
      421 ctaaagtgct ccagatccca agagaaatcg aaactggcat tttttccaga accaggtcaa
      481 ccactttctg atctcggaaa aaactttctc cgaaattgaa tgaagcataa tcacgcatca
      541 tcttaaaata tcgaacatgc agcggcttgt caaaaccata catacgctcg atttcttcta
      601 ccagttccgg agcaagtccc tgtgcgccgc gatatttgga gggtttatct gaaacggcgc
      661 tcttttgacc tttaacgtct tcacctccgc ctgagagacg ctcggttgca ctgactgctt
      721 cgccctgtaa ctgggctatc atctgctcca caggacctcc tggagcaatc tggatgatga
      781 tgaagttgac agtcattatt ccaatcagcg ttggaataac gagcaacaaa cgacggataa
      841 tataggcagt catgtagaat tacgcagggc tataaaatcc tcctcagtgt tttgtttatt
      901 ttagatttgg cttttttgcc aacaaagttt tttcttttga ttcatctacc caccaggtaa
      961 aaaatcccaa actatatttg ggagttattt ccgggcggga aaacttgttc cagtatgcaa
     1021 ggcggtaata gttgatgtgc cagtgcggta cgacgtagtg tccccacaac aacgcacggt
     1081 ccagggctct ggtacggttg attaattttt cacgatcagg tgcctcaatt actagccgaa
     1141 ttagtttgtc gattgcagga ttgcagatac cgatgaagtt gcgtgttcca ctggtttcag
     1201 ctgctttaca actccagtag ttatcctgct cattacccgg tgagagggac tgacctatca
     1261 ccatcacaaa catatcaaaa tcaaaggaac ggatacggtt tacgtattga gaagtatcca
     1321 caactcttac actggcctca attcccatgc gttccaggtt tttcttaaag ggcagtacaa
     1381 tccgttcgaa tgcaggggag atcagtaata tttcaaactt aagttgctcg cctgtactgg
     1441 aattgacacg ctttccttct ttaatctccc aaccggcttc tttgaaaagt tttgaagcgg
     1501 cacgcaactg actacgaata tttccagttc catccgttag tgaagggcga aactttttgc
     1561 taaaaacttc ttccggtaga tctttacgga atggttccag aatcgccagt tcttcaggag
     1621 acggtaagcc gcttgatgcg agctcagaat tttcgaaaaa actgtctgta cgtctatact
     1681 gtccataaaa aaggtttttg ttgctccact caaaatcaaa agcatagcta attgcttctc
     1741 tgaccctgcg gtccttgaaa atcgaccggc gtaaattaaa tgcaaagccc tgcattccac
     1801 gtggcagttc atgcgggatt tccgctcgga ttgtgagttt ttcttcaaat gtttttccgg
     1861 aatacattga ggcccacatt tttgaattat tttcctcccg gaagtgatat tccccagctt
     1921 tgaaggcttc cagagatacg gtggcatcac ggtaataatc gtaacgtact gtatcaaaat
     1981 tgttgctgcc tttgttgacg ggatgattcg cggcccagta atccttgaca cgttcgtaag
     2041 ttatgtattt gcctgccttg aaatctttga tacggtacgc tccactgccc agtggtgggt
     2101 cgagagtgga ttttgaaaaa tccctgcttt cccagtaatg cttaggaaga acaggaagct
     2161 gtcctgtaat cagtaccagt tctctgttag taactccttc acggaactgg aatttaacac
     2221 ggcgatctcc aagatctttg acttcgacaa tgtcaccgta ataggatttg taaaaaggag
     2281 cccctttttc gagtaatacg ttgaaggtga aaatcacgtc ttcaaccttg atcggttcac
     2341 catcgtgaaa tttggctttt ggattgaggt ggtaaatcac ccatgtcctg tcttccggat
     2401 actcaatttc atccgcaatc aatccgtatt cggtaaaagc ctcatcggag gaaccggttg
     2461 tcagtgtttc gtaaatcatg ccaagtccat cagcggaaac gcctttggag ataaatccgt
     2521 tgaaactgtc ataagtaccg atggaattca aacgtacaga accgcctttt ggggcgtccg
     2581 gatttacata gtcaaaatgg gtgaagccgt ccggatattt agcagatccg ttcatcgcaa
     2641 tcgaggaagc tttggtaagg tcagaaccgg caaaaactga aatggagtag cttgtgatga
     2701 aggttgctgt gaatataaat gaaattacga gtcgggatat tttcattgtt cctcttttga
     2761 cttatgattg acggggatta aggcgtggtg ccgtttatta ataactgaaa agtatatttt
     2821 ctatcggagg tgggtttatc tcaagaccaa aatgattata ttgtgaattt tgattttttt
     2881 aactgtttag gactaagacg agaatcagta aattcaaaca aatcttgtag agagagtgta
     2941 atctctatgc taaaaatgtt taaagattaa ctattcagca attgtattga gatgaccttt
     3001 atagcgctc
//
LOCUS       DTVD01000033           11673 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1314_length_11673_cov_420.833448, whole genome
            shotgun sequence.
ACCESSION   DTVD01000033 DTVD01000000
VERSION     DTVD01000033.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_11673_cov_420.833448"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            269..850
                     /locus_tag="EYO68_01195"
     CDS             269..850
                     /locus_tag="EYO68_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06450.1"
                     /translation="MASEPKVEELNEDLIDVSAANRDGEAKEANDESEIMSNEPNQDE
                     INVISALEDVPTLNVDEQMLEEESVDNKAEEPSEEAAESTNLPGFLSTEPAEEQEEIF
                     SNQSMPVPEKAKNQVVVIQSFKKYLSGVSSTDDGVYASDITTNNKDAENKTKPKVRPS
                     IKELKNDLILLSKLIKAAPRREKKNSDQKQSQF"
     gene            complement(1037..2365)
                     /locus_tag="EYO68_01200"
     CDS             complement(1037..2365)
                     /locus_tag="EYO68_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC06451.1"
                     /translation="MKFSSIFQANFPFSPLRFPFFYGWCIVIFTTLGMIASIPGQTMG
                     VGVYTDFLIQNSHLTRMQISMAYMTGTILSSLLLPLAGRYYDLVGGRIMIVFAGIGMG
                     ISLLLFAESLTIIHLVHSLLPNISILTSELLVITVIFLLLRQFGQGIMAMVSRNTLAK
                     WFERRRGMVSGISGIFVAFSFSGAPLFMNIIIEDYGYSGSMILMAIIFGFGMAFIGWL
                     FFRDKPEDCGLLMDGDKITSLDTTNLFSEPEITLKEALRTYNFWIFCLGLCSAAVIVT
                     GLTFHISSIGALAGLSRMEAYGLFLPMSVISVISHFFAGWASDRMPLKYLLMILLAGL
                     AVGSLGILNLESFWFRLMLIIGFGVQGGIWGCLTIVAWPRFYGRKHLGAISGAFMGAQ
                     VFASAIGPPIFGLSESLNGDYSSAAWISVALNLLLLLGTVRAVSYYRPLP"
     gene            complement(2619..2695)
                     /locus_tag="EYO68_01205"
     tRNA            complement(2619..2695)
                     /locus_tag="EYO68_01205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(2659..2661),aa:Met,seq:cat)
     gene            2875..3660
                     /locus_tag="EYO68_01210"
     CDS             2875..3660
                     /locus_tag="EYO68_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06452.1"
                     /translation="MISVQNVVKHFGGVAAVDGVDMKIERGSITGLIGPNGAGKTTLF
                     NLIAGNIAPSKGSIFLDGEDVTGLKSHELFSKGLLRTFQIAHEFHTLSVLDNLMLVPP
                     MQTGESLLGAWFRRKEVVRQETEIRKKAEDVISFLNMKHMTAELAGNLSGGQKKLLEL
                     GRTMMVEPKVVVLDEVGAGVNRTLLREIGDAILSMNRDRGYTFCMIEHDMDFISRLCD
                     PVVVMAEGKILAQGSADEIKSNEDVIEAYLGTGLKNKGEVLAG"
     gene            3657..4361
                     /locus_tag="EYO68_01215"
     CDS             3657..4361
                     /locus_tag="EYO68_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_352987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06453.1"
                     /translation="MSYLTGEKLTGGYGGPDIIRECTVSVDQGEIVVVVGPNGAGKST
                     AMKALLGMLDLREGRVLLDGQDISALSPQQRVAKGMAFVPQNQNVFVTMTVEENLEMG
                     AYLRRDDITETMEQVFELFPVLKTKRRQIAGELSGGQRQQVAVGRALMTKPTVLMLDE
                     PTSGVSPIVMDELFDRIIEIKRTGIAILMVEQNARQALNIADRGFVLVTGENRFTDTG
                     EALLANEEVRKSFLGG"
     gene            4365..5387
                     /locus_tag="EYO68_01220"
     CDS             4365..5387
                     /locus_tag="EYO68_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC06454.1"
                     /translation="MEYSTDLLNALVLISNYVLIPAISYGSQLALGAVGVTLVFGILR
                     FANFAHGEMMAFGCMATIFITWWFQSMGISSGVFPTALLALPFAILITALLAVAIDRS
                     IFSFYRIHKSRPVTFVVASVGVMFMLNGLVRILIGTDEIIFRDGQKFLIKAGVFKKMT
                     GLSEGLALKQTQVLTFVLAVIAVLALFWFLQRTRTGKAMRAFSDNEDLALLSGINPER
                     VVLVTWIIAASLATLAGVLYGLDKSFKPMVYFHLLLPIFAAAIVGGIGQPLGAILGGY
                     IVAFSEITATYAFKKFIKYLAPGDFVPDGLAQLLTTEYKFAVSFFILVLVLLVRPTGI
                     LRGRVL"
     gene            5384..6748
                     /locus_tag="EYO68_01225"
     CDS             5384..6748
                     /locus_tag="EYO68_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC06455.1"
                     /translation="MSNNRRAVISFSIMALMIAIVGFGQSWNVALAIVNLCLISAVMS
                     MGLNMQWGFAGLFNAGVMASTAIGGLTALLISYAPVSKAWSVGGTSLFAALISLLLTI
                     VAGVLVWKKTAKGKIRSWRMTAVVLTGYLFMRFFYEQGTAAIEAVDPAKTGFLGGMGL
                     PIILSWFAGGLAAAGLAWLIGRISLGLRSDYFAIATLGISEIMISILKNEDWLSRGVK
                     NVTGLKRPVPYEVDLQQAEWFIKLVHWFYGTAEDGKSLSADVMHEAMQLSAGVFVKLC
                     YSALFLAVVLIILGLATRALNSPWGRMMRAIRDNETAAAAMGKDVTSRHRQVFIIGSA
                     VIGISGAMLTTLDGQFTPGSYIPLRFTFLIWVMVIIGGSGNNLGAIIGAFITWFVWIE
                     AEPSALWLVSHINVFLDESNPLRQHLQEVAPYMRMVLMGLILVLMLRFRPKGILPEEA
                     PDRY"
     gene            6875..8143
                     /locus_tag="EYO68_01230"
     CDS             6875..8143
                     /locus_tag="EYO68_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIC06456.1"
                     /translation="MAININIIREQFPALAITDEGKARVYLDNPGGTQVPRQVLERME
                     RYLIHTNANHGGPFRTSIESDLVLEEAHQAMADLLNAKFADEIVFGANMTSLTFAVSR
                     SLGRLFKRGDTILLTRMDHDGNVGPWLHLAEDLGLKVRWLNFDLKTYEYDLDEAAALL
                     KDGAVKLAAINYASNCLGTINDVKTITKLAHQSDTLVFVDAVQYVPHGPTDVQYIGCD
                     FLVCSPYKFFGPHQGVLWGKAELLSSLEAYKVRPADNTAPGKFETGTQLHEGQAGTLG
                     VLEYLEWIGKTMATEFQANFPDFSGRRKYLHAAMLAIQDYEETLSKRLISGLQNLPGV
                     DVKGISNQNEFSRRVPTVSFTVKNQNPEEIAQKLAEENIFTWHGHNYALEAIRQMDLE
                     KSGGVVRIGPVHYNTIAEIDHTLEVLKTCW"
     gene            8381..9415
                     /locus_tag="EYO68_01235"
     CDS             8381..9415
                     /locus_tag="EYO68_01235"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIC06457.1"
                     /translation="MTFLEDPHRRKNILSGEWVLVSPQRTKRPWNGSVHSPEGKQRTS
                     YDPQCYLCPGNSRAEGKQNPDYKGIFVFANDFPALLPDIEFSESESPLLQVQSVSGIC
                     RVLCFSERHDLTLPELELDAVERVVGVWVEQVKELGGIFKWVQIFENKGEMMGCSNPH
                     PHGQIWAGNFLPNEICREDRQQRDYFQTHGTQLLADYLEHEIRSRERLVEINEHWLAV
                     VPWWAVWPFETLILPKRQVACFSGITGVEQSALAELLKNLLTRYDNLFKTSFPYSMGW
                     HGTPFSSLSKESWTLHAHIFPPLLRSASIRKFMVGYEMLAEAQRDLTPEQAAGRLREC
                     STQHYLKRTL"
     gene            9412..10563
                     /gene="galK"
                     /locus_tag="EYO68_01240"
     CDS             9412..10563
                     /gene="galK"
                     /locus_tag="EYO68_01240"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIC06458.1"
                     /translation="MSKMAIQVQLAFEERFGSAPEHVIRAPGRVNLIGEHTDYSGGFV
                     LPVALEHAVWIALRPRPDQRVVLHSLDFAETAELDLSEPQKQEKGWQEYLKGITKVLQ
                     EAGWKLTGWEGVMMGDIPQGAGLSSSAALEMACLRAFAEASGKSWQPQIAAKMAQQAE
                     TEWVGISCGIMDQLISACGVAGHALFIDCRNLEMKPVKLPEGIRVMILDTMTRRGLVD
                     SLYNERHSQCLQAAEILGKSILREVSLTELQAQSSKLSPLLYRRAGHVISENQRVKDA
                     VGAMRSNDLKLLGQLMQQSHASLRDNFEVSNFALNTMVECALSAPGCLGARMTGAGFG
                     GCAVAIVKTELEIKFYNSVKDCYRKKSSLNPKIISCNPANGVTRLAPLA"
     gene            10810..>11673
                     /locus_tag="EYO68_01245"
     CDS             10810..>11673
                     /locus_tag="EYO68_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06459.1"
                     /translation="MTESLPVSVKTAVPMWNLFRYLRPHWRFFSWSCFSSILNKVLDL
                     MPPVLVGWVIDSVRREPPEWIANLLGTIDPWRLAVFLAVLGVVIFFFESLFQWAYQYG
                     FMNLAQRVQHQLRTDAYNHLQTREIEFFENHRMGETMAMLNDDVNQIERFLNIGFNEI
                     LQLLVLFIFAGSVMLGVSWELAMVGLLPLPFVLCGSLMYQRLISPRYRKVREAVGALS
                     SRLENNIAGILVIKSFTAEKFESGRVADASHQYKQANYQAIKLSALYVPLIRMAIAIG
                     FGGVLLLGSYWV"
BASE COUNT         3309 a         2285 c         2796 g         3283 t
ORIGIN      
        1 tgcagaagaa gagtcaaaag agactgttga agaagaagtg gaaatttatt ttcaaggttc
       61 gaatgagtca actgaaaata ttgtaaaagt tcgtgaaaaa gaaagtcagg aatacagtca
      121 ggttccagca cctgtcaagg aaaaatcatc tcagtcatat aacgctttta ctgagttttt
      181 caaacagact gaagcattaa aaactcaacc ggaagatgtt ccggcaaatc cttcaacacc
      241 tgcagaggta tcaggtttta caggagatgt ggcctcagag ccaaaagttg aagagttaaa
      301 tgaggattta attgatgttt cggcagccaa ccgggatgga gaggcaaaag aagcaaatga
      361 tgagtctgaa ataatgtcaa atgagccaaa tcaagatgaa ataaatgtga tatctgctct
      421 tgaagatgtt ccaacattaa atgttgatga acaaatgtta gaagaagaat ctgttgataa
      481 taaggccgaa gagccttcag aagaggcggc agaaagcaca aatctgcctg gatttttgtc
      541 aactgaaccg gctgaagagc aggaagaaat tttcagtaac caatcgatgc ctgtacctga
      601 aaaggctaaa aatcaagttg ttgttattca gagttttaaa aagtaccttt ccggggtttc
      661 ttcaacagat gatggggtat atgcttcaga tataacaact aataataaag atgctgaaaa
      721 caaaactaaa ccgaaagtac gaccatcaat taaagaattg aaaaacgatt tgatattatt
      781 gtccaagctg attaaggcag ccccgaggcg agaaaagaag aattctgacc aaaaacaaag
      841 tcagttttga aaatcagact tgtggaaatt aattacagac tttgaagagt gttaaggtct
      901 ttcaggatac tttgttcaac aacatttaag gaactgtgaa tttaaatgtg actgtatgtt
      961 taccgacctt ataaatagaa tttagtatga tccaacgaat tgttatggtg ttcagcttga
     1021 gcaaataaaa atatggttaa ggcaatggac gatagtagct gacagcacgt acagttccaa
     1081 gcagcaacag caaatttaag gctactgaaa tccaggcggc tgaagaatag tcaccattta
     1141 agctttccga caaaccaaat ataggcggcc caatcgcact ggcgaaaact tgagccccca
     1201 taaatgctcc actgattgcc ccaaggtgct tgcgtccata gaatcgcggc caggcaacga
     1261 tggtcaggca gccccaaatt cctccctgca ctccaaaacc aataattaac atgagtctga
     1321 accagaacga ttccaaattt agtattccaa gactgcctac agcaagcccg gcaagcagaa
     1381 tcataagaag atacttaaga ggcattctgt cactggccca gcctgcaaaa aagtggctta
     1441 tcacactgat tacagacata ggaagaaata atccataggc ttccattctt gacagtccgg
     1501 caagtgctcc tattgaactg atatgaaagg tgagaccggt gactatcaca gccgcagagc
     1561 ataaaccaag gcagaatatc cagaaattgt aggttcttaa cgcctcttta agtgttattt
     1621 ctggttcgga aaacaagttt gttgtgtcca gagatgttat tttatcccca tccatgagca
     1681 gtccacaatc ttccggtttg tcacgaaaaa acaaccagcc tataaaggcc atgccgaatc
     1741 cgaaaataat ggccatgagt atcatactgc cagaataacc gtaatcttca ataataatat
     1801 tcatgaaaag cggtgcacca gaaaagctga aggctacaaa gatgccactg ataccactga
     1861 ccatgcctcg tctgcgttca aaccatttag ccaaggtatt acggctaacc atggccatga
     1921 ttccctgtcc aaattgtctc agtaaaagaa aaatcacagt gatcactaga agttcgcttg
     1981 ttaatattga aatgtttgga agcaatgaat gtacgaggtg aatgatggtt aatgactcag
     2041 caaacaaaag cagcgaaatt cccattccaa ttcctgcaaa aacaatcatt atccgtccac
     2101 caacaaggtc ataatagcgt cctgcaaggg gaagaagcag actgcttaat atagttccag
     2161 tcatataggc catgctgatt tgcatacggg ttagatgact gttttgtatg agaaaatctg
     2221 tgtaaactcc aacacccatt gtctgtccgg gtatgctagc aatcataccc agggttgtga
     2281 agataacaat gcaccaccca taaaaaaaag gaaaacgtaa aggactaaaa gggaagtttg
     2341 cctggaagat agatgaaaat ttcatttgca agtataaagg tttctcttat tacatctgag
     2401 taaattgttt gtaaaattta aaaatatcaa cttattaaga aaagtcagcg gagattatat
     2461 tgttttcagg aaatcaggaa gtggtcttgg atattgagtt gctggataag gaagaataat
     2521 gaaaatctag aagcataacc agaacacgga accatggata tctatgagga tctactattt
     2581 atcccccaat tgctgggtcg ataaaactat cttaacactg gtagcaggag gcagatttga
     2641 actgccgacc tctgggttat gaatccagcg ctctcaccaa ctgagctacc ctgccatcaa
     2701 gtgctgctag attatgtcat gtaacgcaaa aataagacaa gtttattgtg gattatatta
     2761 gccgtagatt gatttgaaat tatacaaaac taaactttga ttcaatttga cattacagca
     2821 ttttatttgc agaattgggt ggtctgtgaa agtaaagctt agattagatt tgtcatgatt
     2881 tcagtacaaa atgttgttaa gcactttggc ggtgtagctg ctgttgacgg tgttgatatg
     2941 aagattgagc ggggatccat taccggcttg atcggcccaa acggtgctgg aaaaactacc
     3001 ctttttaatt tgattgccgg aaatattgct cctagcaaag gcagtatttt tttagatggt
     3061 gaggatgtga ccggtcttaa gtcgcatgaa ctgttttcca aaggactgct tcgtactttc
     3121 cagatagccc atgaattcca cactctcagc gttttagata atctgatgct tgttccacca
     3181 atgcagacag gtgaatcttt gcttggggcc tggtttcgca ggaaagaggt agtacgtcag
     3241 gaaactgaaa tcaggaaaaa agctgaggat gtaatttcct tcctgaatat gaagcatatg
     3301 actgctgaac tggctggaaa cctttccggt ggtcagaaaa aactgcttga acttgggcgg
     3361 accatgatgg ttgagccaaa agttgttgtg ctggatgaag tcggtgcagg tgttaaccgg
     3421 acactgctca gagaaattgg agatgcaatt ctgagtatga accgtgaccg tggatatact
     3481 ttttgtatga ttgaacatga tatggatttc atcagcaggc tctgtgatcc tgtcgtggtg
     3541 atggcggaag gtaaaatact ggctcagggt agtgcagatg aaattaagtc caatgaggac
     3601 gtcatagaag cttatttggg aaccgggctc aaaaacaagg gtgaggtact ggccggatga
     3661 gttatttgac tggagaaaaa ttaaccggtg gatatggcgg accggatatc atcagggaat
     3721 gtactgtttc agttgaccag ggagagattg tggtagtagt cggccctaac ggtgccggaa
     3781 aatcaactgc tatgaaagct ttgctgggta tgcttgatct gcgtgaagga cgtgtattgc
     3841 tcgatggtca ggacatttct gccctgtctc cacagcaaag agtagctaaa ggcatggcat
     3901 ttgtgccgca aaaccaaaat gtatttgtta cgatgactgt tgaggaaaac ctggaaatgg
     3961 gcgcttatct gcgccgggat gacatcacag aaactatgga acaggttttt gaattatttc
     4021 ccgtattgaa aaccaaacgc aggcaaattg ccggagaact ttctggagga cagcgacagc
     4081 aggttgctgt cggacgcgct ctgatgacaa agcctaccgt tttgatgctg gatgagccta
     4141 cttccggagt ttcaccaatt gtgatggatg aactctttga ccgtattatt gaaatcaagc
     4201 gtaccggaat cgcgattctg atggtagagc aaaatgcccg tcaagctttg aatattgccg
     4261 atcgtgggtt tgttctggtt acaggtgaaa atcgatttac tgacaccgga gaggctttgc
     4321 tggccaatga agaggtccgg aaatcatttt tgggaggata atagttggaa tattcgactg
     4381 acttgttaaa tgcacttgtg ttgatttcaa attatgtttt gatacctgca atatcctatg
     4441 gttctcaatt ggcgctcgga gcagttgggg tcacacttgt tttcggtatt ttgcgttttg
     4501 cgaatttcgc tcacggtgag atgatggcct ttggctgcat ggccaccatt ttcattacct
     4561 ggtggttcca atcaatgggt atttcaagcg gagttttccc aactgcactg cttgccctgc
     4621 cttttgccat tctcatcacg gcactgcttg cagttgcaat tgaccgttca attttcagtt
     4681 tttaccgtat tcataaaagc cgaccggtga cttttgtggt ggcatccgtc ggtgtgatgt
     4741 ttatgctgaa cggcttggtc cggatcctga ttggtacaga cgaaattatc ttccgggacg
     4801 gtcaaaaatt tttaatcaaa gccggtgtct tcaaaaaaat gactggattg agtgaaggtc
     4861 tggctctaaa gcaaactcaa gttttgacct ttgtattggc ggtaatcgca gtactggcac
     4921 tattctggtt tttgcagcgg acacgtacgg gtaaagccat gcgggctttt tctgataacg
     4981 aagacctcgc attgctttcc ggaatcaatc cggaacgggt tgtactggtg acctggatta
     5041 ttgccgcaag tctggcaacg ttagccggag ttctttatgg gctcgataaa agttttaaac
     5101 caatggtcta ttttcatttg ctactgccca tttttgcggc agctatagtc gggggaatag
     5161 ggcagccact gggagcaatt cttggtggat acattgtggc tttttctgaa attactgcaa
     5221 cctatgcctt taaaaagttt attaaatacc ttgcgccagg ggattttgtg cctgatggac
     5281 tggcacaatt attaactacc gaatacaaat ttgcggtatc ttttttcatt ctggtgcttg
     5341 tgcttcttgt acgaccgact ggaatattac gcgggagggt gctgtgagta ataacagacg
     5401 tgccgtaatc agtttttcca ttatggccct gatgattgca attgtgggtt ttggacaatc
     5461 atggaacgtt gcactggcca tagttaatct atgcctgatt tcagcagtaa tgtcaatggg
     5521 tctcaacatg cagtggggtt ttgcaggctt gtttaatgcc ggagttatgg cttctactgc
     5581 aatcggaggt ctgacggcat tgcttatttc gtatgcaccg gtctcaaaag cctggtctgt
     5641 cggtggtact tcactttttg cggcactgat ttcattgctg ctgacaatag ttgcaggggt
     5701 acttgtatgg aaaaaaacag ctaaaggcaa aataaggtca tggagaatga ctgcagtggt
     5761 gctgacaggt tatctcttca tgcggttttt ctatgaacag ggtactgcag caattgaagc
     5821 tgttgatcct gcaaaaacag gttttttggg aggaatgggg ctgccgatta ttctttcatg
     5881 gtttgcaggt ggactggctg cagcgggttt agcctggctt attggaagaa tttcacttgg
     5941 actgcgctct gattattttg caattgcgac actcggcatt tcagaaatca tgatttcaat
     6001 cctcaaaaac gaggattggc tgtcccgtgg tgtaaagaac gtgaccggac tgaaacgtcc
     6061 tgtgccttat gaggtggatc ttcagcaggc agaatggttt attaagctcg tgcattggtt
     6121 ttacggaact gctgaagacg gcaaatctct cagtgcagac gtaatgcacg aggcaatgca
     6181 gttaagcgca ggcgtgtttg tgaaactttg ctattcagca ttgtttcttg cggtagtact
     6241 gattattttg ggtctggcca cgcgtgccct gaattcgccc tggggtcgaa tgatgcgtgc
     6301 cattcgtgac aatgaaaccg ctgctgctgc aatggggaaa gacgtaacca gcaggcatcg
     6361 acaggttttt atcatcggtt ctgcagtaat cggaatatcc ggtgcaatgc tgaccacact
     6421 tgatggacag ttcactccgg gcagctatat acccctgcgt ttcacctttc tgatttgggt
     6481 tatggtgatt atcggcggca gcgggaataa tttaggcgca ataattggtg cattcattac
     6541 ctggtttgtc tggatagaag cagagccttc agcgctttgg ctggtgagtc atataaatgt
     6601 ttttcttgat gaatccaatc ctttgagaca gcatctgcag gaagttgcac catatatgag
     6661 aatggtgctg atgggcctta ttctggtgct gatgctgaga ttcaggccaa aaggaatatt
     6721 acccgaagaa gctccggatc gttattgatg cagccttaat aagtccttga gtcctatttt
     6781 tataacaatt gttattccag ccgtaaagat gcgtagacct ggaatcaagt attcatgaaa
     6841 ctcacaaaaa tattaaattt accaagcaaa aatcatggca ataaacataa acataattcg
     6901 agaacagttt ccggcactgg ccataacaga tgagggtaaa gcgagggttt acctcgataa
     6961 tccgggagga acacaagttc cgcgacaagt tctggagcgc atggaacgat atctgattca
     7021 caccaatgcg aaccatggag gtccgttccg gacttcgatt gagtcagatt tggtactgga
     7081 agaagcacat caggcaatgg cggatttgct gaatgcaaaa tttgcagatg aaattgtgtt
     7141 tggtgcgaat atgacaagct tgacctttgc agtttcaagg tctttagggc gtcttttcaa
     7201 acgcggagac actatattgc tgaccagaat ggatcatgac ggcaatgttg gtccatggct
     7261 gcatctggct gaggatctgg gtttaaaagt caggtggctg aattttgatt tgaaaacata
     7321 tgaatatgat cttgacgaag ccgcagcttt actcaaggac ggtgcagtaa aactggcggc
     7381 aatcaactat gccagcaatt gtctgggcac aattaatgac gtgaaaacaa tcacaaaact
     7441 ggcccatcag tccgacactc tggtttttgt agatgcagta cagtacgtac ctcacgggcc
     7501 tactgacgtt cagtatatcg gttgtgattt tctagtctgt tcaccctata aatttttcgg
     7561 tccgcaccag ggggttttgt gggggaaagc agaactgctc tcttcactgg aggcctataa
     7621 agtaaggcct gcagacaata ctgcaccagg aaaatttgaa actggaaccc agttgcatga
     7681 aggccaggca ggcacattgg gcgtactgga atatctggag tggatcggca aaacgatggc
     7741 gacagaattt caggcgaatt tccctgactt cagtggtcgc cgaaaatatt tacacgccgc
     7801 aatgctggct atccaggact atgaagagac tctaagcaaa agactgattt ccggtctgca
     7861 aaacttgcct ggagtagatg taaagggaat cagcaatcaa aatgaattct cccgccgtgt
     7921 tccaactgtt tcatttacgg tcaaaaatca aaatccggaa gagattgcgc agaaattggc
     7981 tgaagaaaat attttcacct ggcacggaca caactatgct ctggaagcaa ttcgtcaaat
     8041 ggatctggaa aaatcaggag gtgttgttag aataggcccg gtgcattaca ataccattgc
     8101 tgaaattgac cacacacttg aggtcttgaa aacctgctgg taaagaatta tagccgtagt
     8161 agttcatttc aactgtatta accttgtcta caatttgtct taatttatta attgcaaagt
     8221 tcttactcaa atgctttcat tcctttggaa acacatatga tccgatgtga ttgctgagct
     8281 aaatatgaga ctcctcgaga attagttcta ttcttttgta gtatattttt catccaggag
     8341 agtccactcc ttaaaaccaa tgatttctgc agattacagc atgacatttc ttgaagatcc
     8401 tcatcgtagg aagaacatcc tcagcggcga atgggtactg gtttctccgc agcgtaccaa
     8461 gaggccctgg aatgggagcg tacattctcc ggaaggtaaa caacgcacca gttatgatcc
     8521 gcagtgttac ctctgtccag gaaactcacg ggcagaggga aaacaaaatc ctgactacaa
     8581 aggaattttt gttttcgcca atgattttcc ggctttgctg cctgatatag aattcagtga
     8641 atccgaatca ccacttttac aagttcaatc agtttctggc atttgccgcg tgctttgttt
     8701 ttcggaaagg catgacctga cgcttccgga attggagttg gatgcagtgg aaagagtggt
     8761 gggggtatgg gtggaacagg ttaaagaatt gggaggaatc ttcaaatggg tacagatttt
     8821 tgaaaacaag ggagaaatga tggggtgctc caatccccat ccacacggtc aaatatgggc
     8881 tggtaatttt ctgcccaacg agatctgtag agaagataga caacaacgtg attacttcca
     8941 aactcatgga actcaacttt tagctgatta ccttgaacat gaaataagaa gccgggagag
     9001 attggttgaa ataaatgaac actggctggc agtagttccc tggtgggcag tttggccttt
     9061 cgaaacactt attttaccaa aacgacaggt ggcttgtttt tcaggtatta ccggagttga
     9121 gcagtctgcg ctggcagaat tgctgaagaa tctactgacg cgatacgaca atctttttaa
     9181 aaccagtttc ccttattcga tgggatggca cggtactcct ttttcatcgc tctcaaagga
     9241 gtcatggacg ctacacgcgc acatctttcc tccgttattg cgttcagcca gtatccgaaa
     9301 attcatggta ggttacgaaa tgcttgctga agcacaacgt gatttgactc cagaacaagc
     9361 ggccggacgc ctccgtgaat gttcgactca gcattatctt aagcgtactc tatgagcaaa
     9421 atggccattc aagtccaact agcttttgaa gaacgttttg gttctgctcc agagcatgtg
     9481 attcgggcac caggaagagt caatttgatt ggtgaacata ctgattacag tggagggttt
     9541 gttttgcctg ttgcacttga acatgctgtt tggatcgccc ttcgtcctcg cccggaccaa
     9601 cgtgttgtac tgcattctct ggattttgct gaaaccgcgg aacttgatct ttcggaaccg
     9661 caaaaacaag aaaaaggctg gcaggagtac cttaagggga tcaccaaggt cctgcaggag
     9721 gcaggatgga aactaactgg ttgggaagga gtgatgatgg gagatattcc acagggagct
     9781 ggactttcct cttccgcagc acttgagatg gcctgtctca gagcttttgc agaagcttcc
     9841 ggaaaatcgt ggcagcccca aattgcggca aaaatggcac aacaggcaga aactgagtgg
     9901 gttggaatta gctgcggtat tatggatcaa ctgatttccg catgtggtgt tgctggacat
     9961 gcgcttttta tcgattgccg taatttggaa atgaaaccgg taaagctgcc ggaaggaata
    10021 cgagtcatga ttctcgatac tatgacacga cgtggcctgg ttgactcact ttataacgaa
    10081 cgacacagtc aatgccttca agctgccgaa atcttgggaa aatctatcct gagagaagtg
    10141 tctcttacag aacttcaagc tcaatcgtca aagctaagtc ctttactcta tcgccgggct
    10201 ggacatgtca tcagcgaaaa tcagcgtgtc aaagatgctg taggagcaat gcgaagcaat
    10261 gatctcaaat tacttggtca actgatgcag cagtcacatg ctagtttgcg tgataacttt
    10321 gaagtgtcca atttcgcttt gaatacaatg gtggaatgcg ctctctctgc tccaggatgt
    10381 ctgggggcaa gaatgacagg tgcgggcttt ggaggctgtg ctgttgcaat tgttaaaaca
    10441 gaacttgaaa taaaatttta taactcggtt aaggactgct accgaaaaaa atcaagtttg
    10501 aatccgaaaa ttatttcctg taatcctgcc aatggtgtga ctagattagc acctctcgcc
    10561 taataattat caatgttcaa aattctgcca atactaaggt aaattattgc tgaagttttg
    10621 ggggaagata ataatttaat cttataaatt aaagtgtatt tgcttgattt actggaatag
    10681 tttagctata actcacgtat gtatttttag gtatttcatc agatttgttt ttggctctct
    10741 ccgcaaagtg tcacatagag tgcttaattc gcgctctcaa tataattaaa aatatataat
    10801 tttgcttaaa tgacagaatc attgcctgtt tcggttaaaa ctgccgttcc tatgtggaat
    10861 cttttccgtt atctgcgtcc gcattggcgg tttttttcgt ggtcctgttt ttccagtata
    10921 ttgaataagg ttcttgacct gatgccgcca gtactggtgg gctgggtgat tgactccgtc
    10981 aggcgtgagc ctccggagtg gattgcgaat ttactcggaa ctatagaccc ctggaggttg
    11041 gcggtattcc tggcagtgtt gggtgtagta atttttttct tcgaaagtct tttccagtgg
    11101 gcttatcagt acggattcat gaatcttgcc cagcgtgtac agcatcagtt gcgcacagac
    11161 gcctataatc acttgcagac gcgtgagatt gagtttttcg agaaccaccg aatgggggag
    11221 acgatggcga tgctgaacga tgatgtgaac cagattgagc gtttccttaa catcggcttt
    11281 aacgaaatcc tgcaattgct tgtacttttc atttttgccg gcagtgtaat gttaggagtt
    11341 tcatgggaat tggccatggt ggggttattg cctctgcctt ttgtactttg tggaagcctg
    11401 atgtaccagc gcttaatatc tccacgctac cggaaggttc gagaagcagt cggtgcgctc
    11461 agtagccgtc ttgagaataa tattgcaggt attcttgtaa tcaagagttt cactgcagaa
    11521 aaatttgaat ccggacgcgt ggctgatgct tcacatcaat ataaacaggc aaactaccag
    11581 gcgatcaagc tcagtgcgct ttatgtaccg cttattcgta tggcgattgc aattggtttt
    11641 ggcggtgtac ttttactggg gagttactgg gtg
//
LOCUS       DTVD01000034            2996 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13192_length_2996_cov_258.226114, whole genome
            shotgun sequence.
ACCESSION   DTVD01000034 DTVD01000000
VERSION     DTVD01000034.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13192_length_2996_cov_258.226114"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="EYO68_01250"
     CDS             <1..384
                     /locus_tag="EYO68_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIC06460.1"
                     /translation="LGNIASARNQREGIPVVQSNTYGMTYGALLMLVLAWSTGREFNF
                     EFTVSYVSSLVFLSVFASIIAFWSYLTLLGRVGVERAAYATLIFPLVALGISTIFEGY
                     QWSAYAVIGIVLILGGNLLILKRSI"
     gene            complement(376..1392)
                     /locus_tag="EYO68_01255"
     CDS             complement(376..1392)
                     /locus_tag="EYO68_01255"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIC06461.1"
                     /translation="MEEFIKALPKAELHLHIEGSLEPELMFELAERNNVPLPFASVEE
                     VQAAYVFSDLQSFLDIYYAGAGVLLQVSDFFDLTWAYIQRVQKQNVRHIEIFFDPQAH
                     TDRGIPFQIVITGIHKALVEAKQKFGITSRLILCFLRHLSEEAALATLADALPFRGWI
                     EGVGLDSSEVGFPPEKFERVFAKATDAGLRKVAHAGEEGPPEYIWQALDLLGVSRIDH
                     GVRCMEDPKLVERLAKEKIPLTVCPLSNTKLRVFDKMENHNLKQMLDAGLCVTINSDD
                     PAYFGGYMNENFLAAQKGLNLSQKDIVQVAKNGFTASFLPQEDIDVHLKSIDDFVAEQ
                     IVSD"
     assembly_gap    1679..1778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1825..2562)
                     /locus_tag="EYO68_01260"
     CDS             complement(1825..2562)
                     /locus_tag="EYO68_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC06462.1"
                     /translation="MPGSVLITGASTGIGRALAFEFSRRGFALGLCARRLELLEELEE
                     ELSGENQVAVAHLDVAELESVPEVLNKLADELGGVSIIIANAGIGGRSYPGEGTFHQD
                     RKIIEINVLGAMATIDAGADILKKQGGGQIVGISSIAGFRGLSATPAYSASKAALSTY
                     MEGIRNHLLQHKIAVTVINPGFIDTPINTHRKSRPFLISAEKGAQLMADMIEKQVLSS
                     TVPVWPWAVISRIMYLIPERLWCRINV"
     gene            complement(2564..2770)
                     /locus_tag="EYO68_01265"
     CDS             complement(2564..2770)
                     /locus_tag="EYO68_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06463.1"
                     /translation="MENLIQGTSTKLKEWLALFQRQRQVRYGVLVVYLLIWLFSSGSL
                     STTIWVGVGVGLGWISGRDNEQEN"
BASE COUNT          786 a          652 c          601 g          857 t
ORIGIN      
        1 ctgggtaata tcgcttctgc cagaaatcaa cgcgagggta ttccggtggt acagtcaaac
       61 acttatggga tgacatacgg agcactgctg atgcttgtat tagcatggtc aacaggacgt
      121 gaatttaatt ttgagttcac tgtttcgtac gtcagttcgt tggtgtttct ctccgttttt
      181 gcatccatca ttgccttttg gtcttacctc acattactcg gacgggtagg ggtggagcgt
      241 gcagcatatg caaccttgat ttttccttta gttgcactgg gaatttcaac aatattcgaa
      301 ggttatcaat ggtctgcgta tgcagtaatc ggcatagtat tgattctagg aggtaactta
      361 ttgattctca aacgttcaat ctgaaactat ttgttcagca acaaaatcat caatggattt
      421 caggtgaacg tcaatgtctt cctgtggcag aaaactggct gtaaatccgt ttttagctac
      481 ttgaacaatg tctttctgac tcagatttaa acccttttga gcggccagaa aattctcatt
      541 catgtagccg ccaaaataag ccggatcgtc ggaatttatg gttacgcaaa gtccggcatc
      601 cagcatttgt ttgaggttgt gattctccat tttatcgaaa acacgtaatt ttgtgtttga
      661 aagtggacag acggtcaggg gtattttttc tttagcgaga cgctccacga gttttggatc
      721 ttccatgcag cgcactccat ggtcaatccg ggaaactccg agtaaatcca aagcctgcca
      781 aatgtattcc ggaggccctt cttcgccggc atgtgcgact tttcgtaaac ctgcatcagt
      841 tgctttggca aaaacccgtt caaacttttc cggagggaat ccgacttcag atgaatccaa
      901 cccaactcct tcaatccacc cacggaaagg cagagcatct gctaaagtcg ctaaagcggc
      961 ttcctcgctt aaatgcctta aaaaacacag aatcaaacgc gaagtaattc cgaatttttg
     1021 ttttgcttca accagtgctt tgtggattcc ggtgatcaca atttggaaag gaatacctcg
     1081 atcggtgtgg gcttgaggat caaaaaagat ttcaatatgc cgcacatttt gtttttgaac
     1141 gcgctgtata taagcccatg tcaaatcaaa aaaatcagac acctgtaaaa gcactcctgc
     1201 gccggcatag taaatatcca aaaatgattg taaatcactg aatacatatg ccgcctgtac
     1261 ttcctcaacg gatgcaaacg gcaaaggtac attgttccgt tcagccaact cgaacatcag
     1321 ttcgggttca agagaaccct ctatgtgcag gtgtaattcg gcttttggca gtgctttgat
     1381 aaactcttca atcatttctc tctcctcttg attaagactg aaaattagac acatcctccc
     1441 gatggtactt tgttcagacg cgaatcgcaa ttagtctgca aaactggttc ctttttttta
     1501 tttactatat acccccttac agtataatgg aattacatta acaagcttta taacttgttc
     1561 attatgtttc atcaagcaga gattacaaac aatattagca atattagttt tttgaattaa
     1621 acagctgcga acacatttta aacgtagctt taagccaacc ttactcaaca cggaatatnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aatatctaaa acaaatgatg
     1801 ctttttggag caactctgaa ttactcagac gttgattctg caccagagac gttccggaat
     1861 caggtacatt attcgggaaa ttactgccca tggccaaacc ggaacggtgg aggacaaaac
     1921 ctgcttttca atcatgtcag ccattagctg agctcctttt tctgcagaaa taagaaaggg
     1981 gcggcttttt cgatgggtat tgatgggagt gtcaatgaat cccggattga tgacagtgac
     2041 agctatcttg tgctgcagaa ggtgattccg gataccctcc atgtaagttg aaagtgctgc
     2101 cttggaagca gaataagccg gggttgcgga aagtccccta aaacctgcga ttgaagaaat
     2161 tcctacgatt tgtccgccgc cctgtttttt tagtatatct gcacctgcat caatggtggc
     2221 cattgcgcca agtacgttaa tttctattat cttacggtcc tgatgaaagg tgccttctcc
     2281 cggataactt cttccgccga taccggcatt tgcaattata atactgactc caccaagttc
     2341 atctgcaagc ttgtttaaga cttccggaac actttccagt tccgcaacat caagatgagc
     2401 aaccgcaacc tgattttcac cggacaattc ttcttcgagt tcctccagca attccagacg
     2461 tcttgcacat aagcctaagg caaaacccct gcgtgaaaat tcaaaagcca gagcccgtcc
     2521 aattcctgtg ctggcaccgg ttattaagac tgaacccggc attttaattt tcttgctcgt
     2581 tgtctcttcc gctaatccag ccgagtccga ctccgactcc aacccagatg gttgtagaaa
     2641 gacttccgga tgaaaagagc cagataagga gatagacgac aagcacacca taccggactt
     2701 gacgttgtct ttgaaaaagt gcgagccact cttttaattt tgtggaagtc ccctggataa
     2761 ggttttccat gtaattctcc ttttaattaa tttttatcga ttttaaggat tgcagctttc
     2821 agtctccagc ttacctgtga cagcttcaag tttaacttga atgcacaaaa tagttgctac
     2881 tttattctca atacgatagc attaatgatc tgtgagtgag gccgtagtgg cggaattggt
     2941 agacgcgcta ggttcagggt ctagtgttca ttggacgtgg gagttcgagt ctcccc
//
LOCUS       DTVD01000035            2990 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13223_length_2990_cov_389.210221, whole genome
            shotgun sequence.
ACCESSION   DTVD01000035 DTVD01000000
VERSION     DTVD01000035.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_2990_cov_389.210221"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="EYO68_01270"
     CDS             complement(<1..1814)
                     /locus_tag="EYO68_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIC06464.1"
                     /translation="MKKHYISGIIIFVLGTAFSTNVFAEGDLGRGEAKYKVCAACHGE
                     NGEGRKIANAPRISGQHSWYIARQLNNFKNGVRGTHINDITGMQMRPMAMTLLTDQDI
                     DDVLVYVGSLNGRGKHSGVKGDIKKGKLAYAVCVTCHGANGQGNEALNAPKIAGLPDW
                     YVERQLNNFKSGIRGVHAKDIFGQQMRPMAMTLASDEAVRDMTVFVASLKSSSISKPV
                     TVASSSTAVTNVQAPVSEPLYAPCASCHGSEGEGNQKLGAPRLAGQHAWYLERQLRNW
                     RDGIRGTHSEDLYGIQMRPMAMTLVKDSDLKKVVSFIGTLRGEPSKSTIQGNSDSGKT
                     SYATCIACHGEQGEGNKALNSPKIAGLQDWYIARQIRSFKKGIRGSHEKDVYGMQMRP
                     MAMTLADDESIKNVALYVSTFKEKAQPAITQTAKISTAEPASVSVTSSTENGKTLYGV
                     CASCHGADGAGNKALNAPRISGQKAWYIARQLANFKSGIRGSHEKDIYGQQMRPMSMT
                     LANDQMVADVSAYVSTLKSPASPPTIKGDAAAGKAAYAVCASCHGANGEGNKALNAPA
                     IAGQNDWYIVRQLYNFKNGIRGADPKDSYGQQMRPMAMT"
     gene            complement(1964..2203)
                     /locus_tag="EYO68_01275"
     CDS             complement(1964..2203)
                     /locus_tag="EYO68_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06465.1"
                     /translation="MFNNFVSSFPRFLLCVLLFSIFLLEINGCETSLNQKNYLECRQM
                     LEKDRQRVRAGNGFSYNSQSFEECRASARFQTDAD"
     gene            complement(2220..>2990)
                     /locus_tag="EYO68_01280"
     CDS             complement(2220..>2990)
                     /locus_tag="EYO68_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIC06466.1"
                     /translation="PEKLREVLRPETSLISVMTANNETGNLQPIEEISKIAQENKIPF
                     HTDLVQAYGKMQLDLSLSGVNFASATAHKLGGPCGIGLLFVKQGSVFQSLISGGKQER
                     VRRAGTENVILAVGFAKAVEWYLENQLSLRDRFSKFRDLILNEINKIKGIFLNTDLGN
                     SMPHTLNFGCHGISAESLLISLDLDGIAVSTGSACSSGAMEASPVLLAMGLSRAEAKS
                     SLRVSWGWSTTEADIDFFCQRLTFHIQRLQEKQITEKL"
BASE COUNT          827 a          683 c          577 g          903 t
ORIGIN      
        1 gtcatggcca ttgggcgcat ttgctgacca taagaatctt ttgggtccgc accacgaatt
       61 ccatttttaa aattatatag ttgtctgacg atataccagt cattttggcc agcaatagct
      121 ggagcattca gagctttgtt gccttcacca tttgcaccgt gacaggaggc acagactgca
      181 taagcggctt tccctgcggc agcgtcgcct ttaatcgttg gaggagaagc tggacttttc
      241 agagtactca cataagctga tacatctgca accatctgat cgttggcaag cgtcatagac
      301 atcggtcgca tttgctgtcc atatatgtct ttctcatgag atccgcgtat acctgatttg
      361 aaatttgcca attgtcgggc aatgtaccat gccttttgtc cagaaattct cggtgcgttt
      421 agagccttgt ttccagcacc atcagctccg tgacaggaag cacatactcc atataaagtt
      481 ttgccatttt cggtagaact ggtaacagac actgaggctg gttcagcagt actgatctta
      541 gcagtttgtg taattgcagg ctgagctttt tctttaaaag ttgacacata cagtgcaaca
      601 tttttgatag attcatcgtc agccaaagtc attgccatgg gccgcatttg cattccataa
      661 acatcttttt catggcttcc acggatacct tttttaaaac tgcgtatctg ccttgcgata
      721 taccaatctt gaagtccggc tatttttggt gaattcagtg ccttgttccc ctcaccctgt
      781 tcgccatgac aagctataca agttgcataa cttgtttttc cactgtcact gtttccctgt
      841 attgttgatt ttgaaggttc tcccctaaga gttccaataa aagacactac ttttttcaag
      901 tctgaatctt ttaccaaagt catagccatc gggcgcattt gaatgccata aagatcttcg
      961 gaatgtgttc cacggatacc atcgcgccag ttacgtaact gacgttcgag ataccaggca
     1021 tgctgacctg ccaaacgcgg cgctccaagt ttttgattgc cttcaccctc cgatccatga
     1081 caggaagcac agggagcata aagtggttca cttacaggtg cttgaacatt agttacagcg
     1141 gtgctggaag aagccacggt tacaggtttg gaaattgaac tgctttttaa agacgctaca
     1201 aaaaccgtca tgtcacggac cgcttcatcg cttgcaagag tcatggccat cgggcgcatc
     1261 tgctggccaa aaatatcttt ggcatggaca ccacgaatgc cgcttttgaa attattcaat
     1321 tgcctttcaa cataccagtc aggaagtccg gcaatttttg gtgcattcag tgcctcatta
     1381 ccctgaccgt ttgcgccatg acaagtgaca caaacagcat aagccaattt ccctttcttg
     1441 atatctcctt ttacaccgga gtgttttcct cgaccgttta ggcttcccac atacactaaa
     1501 acatcatcaa tgtcctgatc tgtcaagaga gtcatagcca tcggacgcat ttgcatgcca
     1561 gtaatatcat taatatgtgt tccacgcaca ccatttttaa aattattgag ctgtctagct
     1621 atataccagg aatgttgacc tgaaattctg ggagcatttg cgatttttct gccttcgcca
     1681 ttttctccgt gacaggcagc gcataccttg tactttgctt ctccacgtcc caagtctcct
     1741 tctgcaaaga catttgtcga aaatgcagtt cctagaacaa aaataattat tcctgaaata
     1801 taatgcttct tcatgacaat tctttcgtaa atcaaaacct tgtaagctga gtcagttcaa
     1861 aagataattt ttgactttat gaatttatat aacaaccgag aactctagca aaattttgaa
     1921 aaattggcaa atttattggt atctttgaaa aatatttaac ggattaatct gcatctgttt
     1981 gaaaacgtgc tgaagcccgg cattcttcga atgactgtga attgtaactg aatccatttc
     2041 cagcacgtac cctttgacga tctttctcaa gcatctggcg gcattccaga taatttttct
     2101 ggttaagcga agtttcacat ccattgattt ccagcaaaaa aatactaaaa agcaatacac
     2161 acagtaaaaa acgaggaaaa gagctcacaa aattattgaa cacaaagaaa ctttttggtt
     2221 tagagttttt ctgttatttg tttttcctgc aaacgctgaa tgtgaaaagt caggcgttgg
     2281 caaaaaaaat caatatcagc ttcagtcgtt gaccaacccc aactgaccct caggcttgat
     2341 tttgcttcag cacgagaaag gcccatggcc aaaagcacgg gactggcttc catggctccg
     2401 gaagaacagg ctgagcctgt agacactgca attccatcca aatccagtga aatcaaaaga
     2461 ctttctgcgc taattccgtg gcagccaaag ttgagagtat ggggcatact atttcccaaa
     2521 tcagtgttta gaaatatgcc ttttatttta ttgatttcgt ttagaattag gtcccggaat
     2581 tttgagaagc gatcccggag tgataattga ttttccaggt accactcaac tgcttttgca
     2641 aacccaacgg caaggattac attttcagta ccagctcgtc gaacacgttc ttgtttacca
     2701 ccagagatca atgattggaa tacacttccc tgtttaacaa aaagcaggcc tattccacaa
     2761 ggaccaccaa gtttatgtgc agttgcagaa gcaaaattaa ctccagataa actcaaatcc
     2821 aattgcattt ttccatatgc ctgaacaaga tcggtatgaa atggtatttt attttcttgc
     2881 gcaattttac ttatttcttc tatgggctgg agatttccag tttcattgtt ggcagtcata
     2941 accgaaatca aagatgtttc cggacgaagt acctctcgca atttttccgg
//
LOCUS       DTVD01000036           11646 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1323_length_11646_cov_342.242947, whole genome
            shotgun sequence.
ACCESSION   DTVD01000036 DTVD01000000
VERSION     DTVD01000036.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_11646_cov_342.242947"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="EYO68_01285"
     CDS             <1..926
                     /locus_tag="EYO68_01285"
                     /inference="COORDINATES: protein motif:HMM:PF04577.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HIC06467.1"
                     /translation="VQIMPLILEDALLSAADGLVQSGSKIWFHFQKYPWDLACPEQNP
                     GVGEHRRHIETRLTQGLPDPKLAHVLSGTYHLGISPHIYSYYHLLADLLPHLIASQKF
                     PVLVPEFMPTQFIDLLKEAEFEVQVLSPEFFMVERLLIPEMNFPDWNSEKVKLIQYFF
                     EKIVPLKSLPNSQSSNSEKRIYVSRKLAVKRHLSNEDEFLPLLKKHKFSKIYLEHLSV
                     PEQVQLFRSASHVIAAHGAGLTNILFAPADVKILEIRPVLTSGQFCFENLFSLGWPGS
                     EHLVPPRSSEFALPLELLDEVLERWQGEPKT"
     gene            complement(977..2170)
                     /gene="lhgO"
                     /locus_tag="EYO68_01290"
     CDS             complement(977..2170)
                     /gene="lhgO"
                     /locus_tag="EYO68_01290"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIC06468.1"
                     /translation="MQQTDIIIIGGGIVGLATAYQFTLDYPLLKITLLEKEQQLAAHQ
                     TGHNSGVLHTGIYYKPGSLKALNCLEGKSRMEDFCRKEEIDFEICGKVIVAISEAELP
                     ALETIYQRGRINGVSCEMISTEKLNELEPYVAGIKAVHVPETGIVDYGQVCKRFAKHL
                     QNNEDNQIHCSTKVIGIRHSERIVVETEQGEFEGRFLVNCAGLYSDKITAMTQPPESK
                     IIPFRGEYYEVRPEKHHLCRNLIYPVPDPSFPFLGVHFTRMINGSLECGPNAVLAFAR
                     EGYSRSTVNFLELAEILSYSGFIKLAAKYWRPGVGEMWRSFSKAAFVRALQRLVPEIS
                     ADDLETAPAGIRAQAVLSNGKLVDDFLIQENKNIINVCNAPSPAATSSLNIGKHIVDI
                     VAERF"
     gene            complement(2189..2857)
                     /locus_tag="EYO68_01295"
     CDS             complement(2189..2857)
                     /locus_tag="EYO68_01295"
                     /inference="COORDINATES: protein motif:HMM:PF02589.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HIC06469.1"
                     /translation="MNNTATARENILSNLRISSVVPDITSRKYPVMEYKRWSLQEKVE
                     QLKQKMEAVHTEVLPCSKSSWGRMFRAVVKEKNIEKILYGTNTDLAQKLREMYQQTPE
                     EIPELVYYEKPIEESKDLLFGIDAAITTTLGGIAETGSLILWPTPEEPRLMSLIPPIH
                     IAVLYADTIYNNFLEAMTTENWNSEMPSNALLISGPSKTADIEQTLVYGVHGCKELVV
                     LLIQ"
     gene            complement(2850..4277)
                     /locus_tag="EYO68_01300"
     CDS             complement(2850..4277)
                     /locus_tag="EYO68_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIC06470.1"
                     /translation="MLSPEKFQRDTFSALNNPQLRRNFKRAMSGLMEKRQAVFPDAEE
                     WQLLRELGSLIRANALRKLPELLEQLEANCTANGIQVHWAETVDEANKLVLEIAQRHK
                     VKSVVKGKSMVSEEMELNHYLGQHGIEALEADLGEYIIQVDHELPSHIIMPAIHKNKE
                     QISQMFHEKVDPETLAESAEEMTAIARKVLRKKFYEADMGVSGVNFAVAETGTLCLVE
                     NEGNGRFCTTLPPVHVAVMGIEKVLQRLDDVPPLLSLLTRSATGQAITTYFNMITSPR
                     KSVEKDGPLEVHLILLDNGRSRMHSDELLRDTLLCIRCGACMNHCPVYTRIGGHAYGA
                     TYPGPIGKILTPQIKSLHEAGHLADASSLCGACVEVCPVKIPITDILLRLRHDKAENK
                     RNIPFTESGGLKAKTESLIWKCWGMVYANPILYQLKSYKLSKVGNYMPEWLPLLRNWT
                     SVRSKPRFAKKSLHQLAREEGLIDE"
     gene            complement(4296..5999)
                     /gene="ilvD"
                     /locus_tag="EYO68_01305"
     CDS             complement(4296..5999)
                     /gene="ilvD"
                     /locus_tag="EYO68_01305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIC06471.1"
                     /translation="MKDKTQPKGSGHRRYSKLVTETNARAASRGMLYGVGFKKGDFQK
                     SQVGIASTWGTVTPCNMHINALAEHAARGVRKAKGMPLIFGTITVSDGISMGTEGMKY
                     SLVSREVIADSIETVVGCQSYDGLVTIGGCDKNMPGCLIAIARLNRPAVFVYGGTILP
                     GTHKGQDVDIVSIFEAVGKEAAGSITQNELEEVESCAIPGAGSCGGMYTANTMASAIE
                     ALGMSLPNSSAQEAVSEDKVRDCVEAGEAVLRLIEDNICPRDILTREAFENAITVVMA
                     LGGSTNAVLHLLAMAHAANIKLELDDFLKIGEKAPVLADLKPSGKYFMSNFVKIGGLP
                     PLMKMLLDGGILHGGCMTVTGKTVRENLENVTPYKDDQEIVHSLADPIKKTGHLVILR
                     GNLASEGAVAKISGKEGEYFKGTARVFNSEEEALDRILDGTVVKGDVIVIRYEGPKGG
                     PGMREMLSPTSAIMGRGLGQDVALITDGRFSGGSHGFVIGHITPEAHEGGLLAIVENG
                     DSITIDILNRKIDLEIPEEEVKTRQANWTRPEARYTRGVLAKYAKTVSSASLGAVTDL
                     E"
     gene            complement(6175..7902)
                     /locus_tag="EYO68_01310"
     CDS             complement(6175..7902)
                     /locus_tag="EYO68_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06472.1"
                     /translation="MKQSEWKDHIEFIEAEMLKRGSEQGAPEILKQFGTRLSSTIHHF
                     FAESNSFIPDAPITVEQQAFMHSLQLYDMKSVMRLVANYDATKGLKVVLPGIEKSYRS
                     LMVIQKLEQFTNNSRESTALDAYKSRLEEALSKVLKCRREDLYEEDVLAEKMVVVSGA
                     PEALRNKFFTERLRTLFSSNYRAYLMLKNRYFLKRLKRLSKNPKYYKTQQSHLAKLTS
                     KFKNDEDGSIDLLTENIDFRMIEQLVKEQFSKTSDQAEVREESTKLQTPVDTETEGVP
                     ISDQSKKTETAPQAFTIEEKQVERGNNFDSVSLEISEETMSPYEQLCTSYAEPLEKLS
                     ASFRPVPECSSPYFRSRVEEYRDEFANYFQHLHGGNVQVIAASHNFTLELLMQRGQEY
                     HAAERWGVVPKYKQEEEESIYFRSMEDTARVLDRKAEWSMLIHARLPFRFFFPLEKAK
                     TIEIEKDKLCVFQIGTAFFDQNHQKKLDEYPDVFFKLMFSGHFLTGKNAVIYVEEDPF
                     FTTTLLQMWNCRGLVYLFLMAISHRFAVELSPALQDFFVEVLPNTLHVNGTIAATALP
                     PDLELIKEV"
     gene            complement(7986..9293)
                     /locus_tag="EYO68_01315"
     CDS             complement(7986..9293)
                     /locus_tag="EYO68_01315"
                     /inference="COORDINATES: protein motif:HMM:PF12796.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06473.1"
                     /translation="MRFHLFIYPFLFLAGTLVFAENELDLSQVAGSGTAEQMREMIAS
                     GADLKSKDISGRNALMQAVLFGNHETALVLLENEPDLESKDGLGLTPLTISVRYGDTE
                     MTKILLEHGANVNSTIEDGSTPLYFAALEGQLSAVRLLLEYNSNVNIRTNYGETALIV
                     ASFEGYLGIVRLLLDAGAEINAVNEEGFTALLAAASNGYLEVVNYLTIRGAKSEIVQV
                     QNNLITPENLFGSSREEVDLQVDSTEMRVSEPFYELISASIEGRLSVVNQLLTKGAQV
                     NKSDDVGRTALMEASSFGRYGVVKRLLESGAKVNIADNQKMTALMFATLEGRERVVSL
                     LLDWKADVNAADAEGISSLMLAGNEGRTEIARLLIKHGAKINWQSKSGSSALMEAALE
                     GRTDTVKLLVDANADPSLSDLLERTALVIAEQQGHLDIVEMLK"
     gene            complement(9327..11534)
                     /gene="recD"
                     /locus_tag="EYO68_01320"
     CDS             complement(9327..11534)
                     /gene="recD"
                     /locus_tag="EYO68_01320"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HIC06474.1"
                     /translation="MSSLTDSSELTRPSLISSGWTTAGTARTRFAKESTDDTLKVQIA
                     APALTESLQRLRQQFSQIQLGSEYFFQALDYLCLGESVQKKETEAATLRLHVQLSGLL
                     LMRKAVISGSTCLPWSELQFQIKAILLQTGFFEKEISPDEWEKWLLTSSGTEEGSEGF
                     CAEKSIFVENNSLLYFKKNLSREFKLISLLRMRLDMQPEFPEKSEVENVLKTVLEVNP
                     VRLGEHPLKLAEEQKDAVLSAISNPFLIISGGPGTGKTSVAVTLLRVLKRLGLAKRPA
                     LAAPTGRAAKRMSEAVVSSLRSLENLEQLEEDLELLDVAAEAKTLHRLLEYYPVERSF
                     RHHEYAPLEHDLLIIDEGSMIDQDLMISLLRAAFSELQYQSSVPRIILLGDTQQLPSI
                     GNGAVLQELTAENSDSVPEVAVDNPVPVVRLVHSYRQKISDPAGRNILGVASTVKEME
                     LNPRPELLFETESPNHEIIRRLNGLEEAESEKVMFLNQPNSPNQLLEFAQWWVKRFLR
                     DEKFMFLVQREYLLDAVESDASQLDYLFNYLKRFRILTATQVLSTGAEALNQIILKCW
                     LAENGTKHLFSEHYPGEPVMVSENNYQHKLFNGDQGIFLKFIHPVTKKVELKAVFPVE
                     EEHKTFYVHELHHLHPAYATSVHKSQGSEYDHLALILPAFTTVGENSESEKRTQRELM
                     SREMLYTALTRAKKSVLIMGDQRVLESAAMHKVLRYSGLGAALRSKKHLKVKK"
BASE COUNT         3191 a         2692 c         2243 g         3520 t
ORIGIN      
        1 ctgtccaaat catgccattg attcttgaag atgctttact ttctgcagcc gacggcctgg
       61 ttcagtccgg aagtaaaatc tggttccatt ttcaaaaata tccctgggat cttgcctgcc
      121 cggaacaaaa tcctggtgtt ggagaacatc gaagacacat agaaacccgc ctgactcaag
      181 ggttacctga cccaaaatta gcccatgtcc tttccggaac ttaccattta ggtatttccc
      241 ctcatatata cagctattat catctgcttg cagatttgtt acctcatcta attgcttctc
      301 aaaaatttcc ggtactggtt ccagaattta tgccaactca atttattgat ttattgaaag
      361 aagcagagtt tgaggtacag gtcttgtcac cggaattttt catggtagaa cgacttttaa
      421 ttccggaaat gaactttcct gattggaact ctgagaaagt taaattgata cagtattttt
      481 ttgaaaaaat tgttcctcta aaaagcctgc caaattctca gtcttcaaat tctgaaaaac
      541 ggatttatgt ttcacgaaaa ttagctgtta aacgtcactt atcaaatgaa gatgagtttt
      601 taccattact gaaaaaacac aaattcagta agatttatct ggagcatttg agtgtcccgg
      661 agcaagttca gctatttaga tctgcttcgc acgttattgc agcacatggc gcaggattga
      721 caaatatttt gtttgcgcct gctgatgtca aaattttaga gatacgacca gttctcacaa
      781 gtggccagtt ttgttttgaa aatttatttt ctctgggctg gcccggaagt gaacacctcg
      841 tacctccccg cagcagtgaa tttgcgcttc ctttagaatt actggatgaa gttttggaac
      901 gttggcaagg tgaaccgaaa acctgatttt cctggaagaa taaaataaac accaaaccgt
      961 ctgatcagaa ctcagatcag aaccgttccg ctacaatgtc tacaatgtgt ttgccaatat
     1021 tgagagaaga agttgctgca ggtgatggtg cattacagac attaatgatg tttttgtttt
     1081 cctgaattag aaaatcatca accagttttc cgttggacaa aactgcttgt gctcgaatgc
     1141 ccgccggtgc ggtttccaag tcatctgcag aaatttccgg taccagacgc tgtaatgcac
     1201 gaacaaaagc ggccttgctg aaagaacgcc acatttctcc tactcctgga cgccagtatt
     1261 ttgcagctaa ttttatgaat cctgaataac tcaaaatttc agctagttcc aaaaaattaa
     1321 cagtactgcg actgtaacct tccctggcaa aagccagcac agcatttggt ccacattcca
     1381 gagaaccatt aatcatacgt gtgaaatgta cacctaaaaa gggaaaactt ggatccggaa
     1441 cgggatatat taaatttctg cacaagtgat gtttttcagg ccgtacctcg tagtattcac
     1501 ctcggaacgg aataattttt gattccggag gttgagtcat agctgttatt ttgtcggaat
     1561 ataatccggc acagttaact agaaaacgcc cctcaaattc gccttgctca gtttctacga
     1621 ctatgcgttc agaatggcga ataccaatca cttttgtgga gcaatgaatc tggttatctt
     1681 cgttattctg aagatgcttc gcaaatcgct tgcagacttg tccataatca acgattcctg
     1741 tctccggaac atgaacggct ttaatacctg ctacatacgg ttcaagttcg ttcagttttt
     1801 ctgtactgat catttcacag ctgactccgt tgattcgccc acgttgataa attgtttcta
     1861 aagcaggcaa ctcagcttca gaaattgcta caatcacttt cccgcaaatc tcaaaatcta
     1921 tttcttcttt gcggcaaaaa tcctccatcc tggattttcc ttcaaggcag ttgagcgctt
     1981 ttagagagcc cggtttatag tatattcctg tgtgcaggac tcctgaattg tgtcctgttt
     2041 ggtgggccgc caactgctgt tctttttcca gtagtgtaat tttaagcagt ggataatcaa
     2101 gggtgaattg gtaggccgtg gcgagtccaa caataccccc accgataata atgatgtcag
     2161 tttgctgcat tttgtttggt tctgcacctc actgaatcaa gagcacgacc aattccttac
     2221 agccgtgaac tccataaacg agggtttgct ctatatcagc agtttttgaa ggacctgaaa
     2281 tcaggagcgc attactcggc atttcggaat tccagttttc agtggtcatt gcttccaaaa
     2341 aattgttgta aattgtgtct gcataaagca ccgcgatgtg aatcggcggg atcagcgaca
     2401 tcaaacgagg ttcttccgga gtgggccaaa gtatgagcga accggtttcc gctatgccgc
     2461 caagggttgt agtaatggcc gcatcaatgc caaacagcaa atctttagat tcctcaatcg
     2521 gtttttcata atatactaat tccggtattt cctcaggagt ctgttggtac atctcccgga
     2581 gcttttgggc caaatcagta tttgttccgt aaagtatttt ttcaatattt ttttctttta
     2641 caaccgctct gaacatcctt ccccaggaac ttttcgaaca aggtaaaact tcagtatgaa
     2701 cggcttccat cttttgtttc agctgctcaa ctttctcctg caaagaccag cgcttgtatt
     2761 ccataaccgg atatttccgg gatgtaatat ctggaactac agaggatatt ctgagattcg
     2821 aaagaatatt ttcacgtgca gttgctgtat tattcatcaa tcaatccttc ctcccgggca
     2881 agttgatgta aacttttttt ggcaaaacgc ggctttgagc gtaccgaagt ccaattgcgg
     2941 agaagcggca gccattccgg catataattt ccaaccttgc ttaatttata gcttttcagc
     3001 tgatacaaaa tcggattcgc ataaaccatt ccccaacatt tccaaattaa actttcggtt
     3061 tttgctttca aacctccgga ttcagtgaac ggaatattcc gtttattttc agctttatcg
     3121 tgacgcaggc gcagaaggat atctgtaatc ggaattttga ctggacaaac ctcaacgcag
     3181 gccccgcaca aactcgacgc atctgctaag tgtcccgctt cgtgcagact cttgatttgc
     3241 ggcgtaagaa tttttccgat gggaccgggg tatgtcgcac cgtaggcatg gccgccgatg
     3301 cgagtataaa caggacaatg attcatgcat gctccgcagc ggatgcagag caaagtgtcc
     3361 cgcagcaact cgtcagagtg catccgggaa cgtccattat ccaataaaat taaatgcact
     3421 tcaagtgggc cgtctttctc aactgatttg cgtggagaag tgatcatatt gaaataggtc
     3481 gtgatggcct gtccggttgc ggaacgtgtc aacaaactca acaatggagg aacatcatca
     3541 agccgctgta acactttttc aatacccatc actgccacat gaactggagg caaagtcgtg
     3601 caaaatctgc cgttgccctc gttttcaacc aaacaaaggg ttccggtttc cgccaccgca
     3661 aagttgaccc ctgaaacgcc catatccgct tcataaaact tcttacgcaa aactttccgg
     3721 gcaatggcgg tcatttcctc tgcactctca gctaacgttt caggatctac tttttcgtga
     3781 aacatctgag aaatttgttc cttattttta tgaattgcag gcattattat atgcgaaggc
     3841 aactcatggt caacctgtat aatatattcc cccaagtctg cttcaagagc ttcaatacca
     3901 tgctgtccaa gataatgatt caattccatt tcctcggaaa ccatcgattt acctttgacc
     3961 acacttttca ctttatgccg ctgggcgatt tcaagtacca acttgttggc ctcatcaact
     4021 gtttcagccc aatgaacctg aataccgttt gcggtgcaat tggcctccaa ctgttccagc
     4081 agttccggaa gcttccgtaa agcatttgcc cgaatcaaac tccccaattc ccttagtagt
     4141 tgccattctt ctgcatccgg aaacacggct tgtcgttttt ccatcaatcc ggacatcgcc
     4201 cttttaaaat tacggcgcaa ctgcgggtta ttcagtgcag aaaaagtgtc tctctgaaat
     4261 ttttcagggg aaagcatagg ggctttctat agatttcatt ccaaatcagt taccgcaccc
     4321 agcgatgcag agcttacggt ttttgcatat ttagcaagta ctccgcgggt gtaacgcgct
     4381 tccggacgag tccagtttgc ttgacgtgtt tttacttctt cttccggaat ttccaaatct
     4441 atttttctat tcagaatatc tattgtgatt gagtcaccat tttcaacaat agccagcaaa
     4501 ccaccttcgt gtgcttccgg agttatgtgt ccaatcacaa aaccatgaga acctcctgaa
     4561 aaacgtccat cagtaatcag ggcaacatcc tgacccagac ctcgacccat gattgcagag
     4621 gttggtgata gcatttcacg cattcccggc ccgccttttg gaccttcgta acgaataaca
     4681 atcacatctc ctttaactac ggttccgtct aaaatgcgat ccagcgcttc ttcttcagaa
     4741 ttaaaaacac gtgctgttcc tttgaaatat tcaccctctt taccagaaat tttagcgact
     4801 gcaccttcag atgcaagatt gccacggaga attacaaggt gacctgtttt tttaatggga
     4861 tctgccagtg aatgcacaat ctcttgatcg tccttataag gagtaacgtt ttccagattt
     4921 tctcgaactg tttttccggt aacagtcata cagcccccat gcagtatacc tccatctagc
     4981 agcattttca tcaaaggtgg taatccacct attttgacga aattactcat aaaatatttc
     5041 ccgcttggtt tcaaatcggc cagcactggt gctttttcgc caattttcaa aaagtcatcc
     5101 agttcaagtt tgatgtttgc ggcatgagcc atggccagca aatgcaaaac cgcattggtc
     5161 gaaccaccga gagccataac tactgtgatt gcattttcaa aggcttcgcg tgtcaggatg
     5221 tctcgtgggc aaatgttgtc ttcgatcaag cgcaaaaccg cttcccctgc ttccacacaa
     5281 tcccgaactt tatcctcgga aaccgcttct tgtgcggaac tgttcggtaa gctcatacca
     5341 agagcttcaa tggcggaagc cattgtgtta gccgtgtaca taccaccaca cgatccggca
     5401 cccggaatgg cgcaggattc gacttcttcc agttcgtttt gtgtaatcga acctgcggct
     5461 tctttcccta ccgcctcaaa aatagaaaca atatcaacat cttgaccttt gtgagtcccg
     5521 ggaagaatcg ttcctccata gacaaaaacc gcaggacgat ttagtctggc aatagcaatt
     5581 aaacagccgg gcatattttt gtcacatccg ccaatggtaa ccagcccgtc gtaactttga
     5641 cagccgacaa cagtttcaat tgaatcggca attacttcac gtgaaaccag tgaatatttc
     5701 ataccctcgg ttcccattga tatgccgtct gaaactgtaa ttgttccgaa aattaaaggc
     5761 attccttttg cttttcgaac acctcgtgcg gcatgctcag ctaaagcgtt aatgtgcata
     5821 ttgcatggtg taacagtacc ccatgtggaa gcgatgccaa cctgtgattt ctgaaaatct
     5881 ccttttttga atccaacgcc gtaaagcatc cccctgctgg cagcacgtgc atttgtttca
     5941 gttacaagtt ttgaatagcg acggtgtccg cttccttttg gttgtgtttt atctttcatt
     6001 gaggtcccct ttcggtttaa tgagataagt attaaatctg gtttttagca ttctacaagg
     6061 ccctgtagat tttaatcagc taaacatact atctgtttta tcgcatcaca taaaggaaga
     6121 caagagtttc aacgaagaaa aatatcggga aacaggaatt tccgttcaga attattatac
     6181 ttctttaatt aattctaaat ccggtgggag tgctgtggca gcaatggtgc cgttgacatg
     6241 gagagtgttt ggtaaaactt cgacaaaaaa atcctgtaag gcgggtgaca gttctactgc
     6301 aaagcggtgg gaaatggcca tcaaaaacag ataaaccagt ccacggcaat tccacatttg
     6361 taaaagtgtc gtagtgaaaa aaggatcttc ttcaacataa ataacagcat ttttacctgt
     6421 cagaaaatgg ccgctaaaca tcaatttgaa aaacacgtcc ggatattcgt caagtttttt
     6481 ttgatggttc tggtcaaaaa aagctgtacc aatttgaaaa acacataact tatctttttc
     6541 aatttcgatg gtctttgcct tttccagcgg gaaaaaaaac ctgaaaggca gccgagcatg
     6601 aatcagcatg ctccattctg ctttacgatc caatacacga gccgtatctt ccatcgagcg
     6661 gaaataaata gactcctcct cttcctgttt gtatttaggt actactcccc acctttctgc
     6721 tgcatggtac tcttgtcccc gctgcattag taattccagt gtaaaattgt gacttgcagc
     6781 aattacttgt acatttcctc catgcaaatg ttggaaataa tttgcaaatt catctctata
     6841 ttcttcaacc ctggaacgga aatacggtga agaacattcc ggaactggac gaaaactggc
     6901 ggacaacttt tctaaaggct ccgcataaga ggtgcatagc tgttcatatg gggacatggt
     6961 ttcttcagaa atctctaaac tgactgaatc aaaattatta cccctttcaa cttgcttttc
     7021 ctcaattgta aatgcttgcg gtgctgtctc agttttttta gattgatctg aaattgggac
     7081 accttctgtt tcggtatcta ccggagtttg tagctttgtt gactcttcac gtacttcagc
     7141 ctggtctgaa gtttttgaaa actgttcctt taccaactgc tctatcatcc ggaaatcaat
     7201 attttccgtc aatagatcaa tggatccatc ttcatcgttt ttaaacttag aggtcagctt
     7261 tgctaagtga ctttgctgtg ttttgtaata ttttggattt ttacttaacc gttttaaacg
     7321 tttcaaaaaa taacggtttt taagcattag gtatgcacga taatttgatg aaaataacgt
     7381 tcgcagacgt tccgtaaaaa atttgttccg gagcgcctcc ggagccccgg aaaccacaac
     7441 cattttttct gccagcacat cttcttcata cagatcttcc cgccgacatt tgaggacttt
     7501 actcaaggcc tcttccagac gggatttgta ggcatcaaga gccgtagatt cacgtgagtt
     7561 atttgtaaac tgttctaact tctgaatgac cattaaagaa cggtaacttt tttctattcc
     7621 aggaagtaca acttttaagc ctttggtggc atcatagttc gcaaccaggc gcattactga
     7681 cttcatgtca tagagttgca gggagtgcat gaaggcctgc tgttcaacag ttataggagc
     7741 atcaggtata aatgaattgg attctgcaaa aaaatgatga attgttgagg ataatcttgt
     7801 gccaaactgc tttagaattt ccggagcacc ctgctcactt cctcgtttta gcatttctgc
     7861 ttcgataaac tcaatgtgat ctttccactc ggattgtttc actttgggga tttgagttgt
     7921 agcagattgg atttaaaaag tattagaagt ttcagaagta taaagtgggg tgatggttaa
     7981 aactcttatt tcaacatttc cacaatatcc aagtggccct gttgttctgc gataacaaga
     8041 gcagtccgtt ctaacaaatc tgacaatgat gggtcagcat ttgcgtcaac gagcaattta
     8101 acggtatcgg tgcgcccttc caaagcagct tccatcaatg cggaacttcc actcttgctt
     8161 tgccaattaa tttttgctcc gtgtttaatc aacagccgcg caatttcagt cctgccctca
     8221 ttcccagcaa gcatcagtga ggagatacct tccgcatcag ctgcattaac atcagccttc
     8281 cagtcaagca ataagcttac aacgcgttct cgaccctcaa gtgttgcaaa catcaatgca
     8341 gtcatttttt gattgtccgc aatattaacc tttgcacctg attccagcag acgctttaca
     8401 acaccatatc gtccaaaaga actagcctcc atcagtgcag tacgacccac atcgtcagat
     8461 ttattcactt gagctccttt tgtcaacagt tgattgacaa cactcaagcg accttcgata
     8521 gaagcgctta tcagttcata aaacggttca gagaccctca tttctgtaga atcaacttgt
     8581 aaatcaacct cctcccgact gctgccaaac aagttttccg gtgtaataag attattttga
     8641 acttgaacaa tttcagactt tgcaccacgg attgtgaggt aattaacgac ttccaaataa
     8701 ccattactgg cagcggccaa aagtgctgtg aaaccttctt cattaacagc atttatttct
     8761 gcacctgcat cgagtagcaa acgaacaatt cctaaatatc cctcaaatga ggctacaata
     8821 agagcagttt ctccgtaatt tgtgcggata tttacatttg aattatactc aagcaatagg
     8881 cgtactgcgg acagttgtcc ttcaagtgca gcaaaataca gcggcgttga accgtcttca
     8941 atagtgctgt tcacgtttgc accgtgttcc aacaaaattt ttgtcatttc tgtatcgccg
     9001 taacgtaccg atatggttaa aggcgtcagt cccaaaccat ctttgctttc gagatcaggt
     9061 tcgttctcca gcaagacgag cgcagtttca tgattgccga aaagcacggc ctgcatcaac
     9121 gcgtttcttc cggaaatatc tttgcttttc aagtctgctc cggatgcaat catttcccgc
     9181 atttgctcag cagtaccact tcctgctact tgagacaaat ccaattcatt ttcagcaaac
     9241 acaagtgtgc ctgccaaaaa cagaaacgga taaatgaata aatgaaaacg cattttgggt
     9301 gtttgttctt tttgcttaat gtttttctat tttttcactt ttagatgttt tttgctccgt
     9361 aaagcagcac caagccctga ataacgtaaa actttgtgca ttgcggcaga ctcaagaacc
     9421 ctttgatctc ccattattaa aacagatttt tttgctcttg tcagcgcagt ataaagcatt
     9481 tcgcgactca ttaattcccg ttgagttctt ttttctgact cactgttctc ccccactgta
     9541 gtaaatgcag gcaagatcaa cgccagatga tcatattctg acccctggct tttgtgaaca
     9601 gaggttgcgt aagctggatg caaatgatgc agctcatgca cataaaatgt tttgtgttct
     9661 tcttcaacag gaaaaactgc tttcaactct acttttttag ttacaggatg tataaatttt
     9721 aaaaagattc cctgatctcc attgaaaagt ttatgctgat aattgttttc tgacaccatc
     9781 actggttcac caggatagtg ctccgaaaat aaatgcttag ttccgttttc tgcaagccaa
     9841 cattttagaa ttatttgatt aagtgcttca gctccagttg acaaaacttg agttgccgtt
     9901 aaaattcgaa aacgctttaa ataattaaac aaataatcca gttgagaagc atcagattct
     9961 actgcatcaa gcagatattc ccgctgtacc agaaacataa atttttcatc ccgcaaaaat
    10021 cgcttaaccc accactgtgc aaattctaac aattgattcg gagaatttgg ctgattaagg
    10081 aacatcacct tttccgactc cgcctcttca agaccgttca atctccgaat aatttcgtga
    10141 tttggggatt cggtttcaaa aagcaactcc ggacgcggat tgagctccat ttccttgact
    10201 gtgctggcca ctccaagaat attacgccct gcaggatcac tgattttttg tcggtaacta
    10261 tgaaccaatc ttactaccgg cacgggatta tccactgcaa cttccgggac agaatcacta
    10321 ttttctgcag tcaattcctg caacacagcg ccatttccaa ttgacggcag ttgctgagtg
    10381 tctcccagga gaataatccg tggaacagat gactggtact gcagttctga aaaggctgct
    10441 ctcaacaaag aaatcatcaa atcctgatca atcatcgacc cttcatctat tatcagcaag
    10501 tcgtgttcca aaggggcata ttcgtgatgt ctgaagcttc gctctacagg gtaatactct
    10561 aaaagacgat gaagagtttt tgcttctgca gcaacatcca acaattcaag atcctcttcc
    10621 agttgctcca aattctctag agagcgtaaa ctacttacca ccgcctcaga catccttttt
    10681 gctgcccttc ctgttggagc agccaatgcc ggtcgttttg ctaaaccaag ccgttttagc
    10741 accctgagca aagtgaccgc cacagaagtt tttcctgtac ctggacctcc tgaaataatt
    10801 agaaagggat tggaaatcgc tgacaaaacc gcatcctttt gttcttctgc taattttagg
    10861 gggtgctcac ctaaacgtac cgggttaact tcaagcacgg tttttaggac attctccact
    10921 tcagattttt ccggaaattc cggttgcata tctagacgca tccgtaacag tgaaataagc
    10981 ttaaattcac gcgacaaatt ttttttaaaa tacagaagag aattattttc tacaaaaata
    11041 gatttttctg cacaaaaacc ttctgaccct tcttcagtac cagagcttgt caacaaccat
    11101 ttttcccatt catccggaga tatttctttc tcgaaaaaac ctgtttgtag aagtattgct
    11161 tttatttgaa attggagttc tgaccaagga aggcaggtac ttccagaaat taccgccttg
    11221 cgcatcagga gcaatccgga taactgtaca tgcaaacgaa gggtcgccgc ttcagtctct
    11281 tttttttgga ctgactctcc caagcacaaa taatccaatg cctgaaaaaa atattcactg
    11341 cccagttgta tttgagaaaa ttgctgccta agtcgctgaa gtgactctgt caaggcaggt
    11401 gcagcaattt gtactttgag agtatcatca gtagattctt tcgcaaagcg ggtgcgggca
    11461 gtacctgcgg ttgtccatcc agatgatatt agtgaaggac gtgtgagttc agaagaatct
    11521 gtcaaactgg acattttggc ttgtaagagt tatactatga cttagtttta gggatttatc
    11581 gagtatctcc gtttttagtc tgttctgatt caagctccat cttttttatg ttaaatctgt
    11641 ttctgc
//
LOCUS       DTVD01000037            2986 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13256_length_2986_cov_490.742409, whole genome
            shotgun sequence.
ACCESSION   DTVD01000037 DTVD01000000
VERSION     DTVD01000037.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13256_length_2986_cov_490.742409"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            132..854
                     /locus_tag="EYO68_01325"
     CDS             132..854
                     /locus_tag="EYO68_01325"
                     /inference="COORDINATES: protein motif:HMM:PF01478.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIC06475.1"
                     /translation="MQEITNLAGGAIIGFGLAWMLERWVMSFVRRLSVAEPEELLQEN
                     AENFSKLFSHWGIYIGMTITAAALSEWRQWSPELLNDLILITALVGIAWIDRKTLLIE
                     GRLIALAMGLRLLWLSFFAPQEMLNSLTGLLIGAGILYFIGFLYETFRHRQGLGEGDA
                     AVLGLIGLWVGWQGLGTVLLIAALSGIIIGGISLLRQRKVNQNIPDLLQTQIPFAPFL
                     CLGGLIVYFLQETGFIMMGFGF"
     gene            complement(866..1087)
                     /locus_tag="EYO68_01330"
     CDS             complement(866..1087)
                     /locus_tag="EYO68_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06476.1"
                     /translation="MLISDLKRPCTECDGSGFQAGFDEWGSIQTNLRQSCSGCTGKGY
                     NLTELGDNLWKLYSPMIQDLIREEIQKKG"
     gene            1161..1703
                     /gene="dcd"
                     /locus_tag="EYO68_01335"
     CDS             1161..1703
                     /gene="dcd"
                     /locus_tag="EYO68_01335"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIC06477.1"
                     /translation="MFLFILKVIIPDIGIKKLVEDGFLPPGLTIGPSSVDLTLSDSFS
                     WPKPDKDKIILGESVPHKQVQKDEFVLEPNHFVLASTAESIRVPHDMAAYVEGRSSIG
                     RLGLQVQNAGFIDAGFHGQITLELENQSGFPIVLKKGVRICQIVFVQMSRSAEQPYNG
                     KYCGQSGATPSRLEVDPEFT"
     gene            1812..>2986
                     /locus_tag="EYO68_01340"
     CDS             1812..>2986
                     /locus_tag="EYO68_01340"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC06478.1"
                     /translation="MEKVKFFDTTLRDGEQSPGCSMSADEKLTLAKQLERLGVNTIEA
                     GFAASSPGDFESVREIGRQITNSTVVSLCRASKNDIDSAIEALGDAKNWGIHTFISTS
                     ALHMKHKLQMEPKDVKAMAVAAVERAKKATDNVEFSPEDATRSDPEFLVEVLSETVRA
                     GATILNIPDTVGYTTPDEMFDLITRLRNEVYNADKVTFSVHCHNDLGLGVANSLAAVR
                     AGARQIECTINGIGERAGNASLEELVMALKTRQEYFGFESDIVTEQLFPSSKLLSKIT
                     GVDVQPNKAIVGANAFAHEAGIHQHGVLKNPLTYEIMTPQSVGIKSSNLVMGKHSGRF
                     ALGERIKELGFDLNDIDLNHIFKEFKRLADLKKQVFDEDIEALVTHGIGKQTDKYSL"
BASE COUNT          829 a          600 c          705 g          852 t
ORIGIN      
        1 ctaaaagtaa gattcacaag ccactgaaaa atgatggcaa cagagagtac tttaattaag
       61 tccgtgcctg acggaaatac ccaacgattc ctcagtttca taaattgcta aaactaaaac
      121 cataatcctt aatgcaggaa ataaccaacc ttgccggagg cgcaatcatt ggatttggat
      181 tagcctggat gcttgaacgc tgggtgatga gttttgtccg gaggttatcc gtagctgaac
      241 cggaggaact tttgcaggaa aatgccgaaa atttttcaaa acttttttcg cactggggaa
      301 tttatattgg aatgacgatt acggccgcgg ccctcagtga atggaggcag tggagccctg
      361 aacttctaaa tgatcttatt ttaatcacag cacttgttgg aattgcctgg attgacagaa
      421 aaactctatt gatagaagga cggctgatag ctttggcaat gggactgcgt ttgttatggc
      481 tttcattttt tgcgcctcaa gaaatgctga attccctgac cggattgctg attggagccg
      541 gaattcttta tttcattggt tttttgtatg aaactttccg ccaccgtcag ggactgggtg
      601 aaggtgatgc tgcagtgctt ggtttgattg gtttgtgggt tggctggcag ggactgggaa
      661 cggtcctgct gattgcagct ttgagcggaa tcattattgg cggaatttca ttgctcagac
      721 aaagaaaggt aaatcagaac attccggatc tgctgcaaac ccaaattccc tttgcaccgt
      781 ttctttgtct tggtggattg atagtctatt ttcttcagga aactggattt attatgatgg
      841 gctttggatt ttaatcattc ggcattcagc cctttttctg tatttcttca cgaatcaagt
      901 cctgaatcat cggactgtat aatttccaca aattatctcc cagttctgta agattatatc
      961 cttttccggt gcaacccgaa caggactggc ggaggttagt ctggatgctt ccccattcat
     1021 cgaatcctgc ctgaaatccg ctgccgtcac attcagtgca tggtcttttt aaatctgata
     1081 ttaacatttg ctttcaagtg tgttcttagt atgatatgtt tattgtagta aacgcatgaa
     1141 ttatgccttg atttaaccta atttttcttt tcattctgaa agtgattatt cctgatatcg
     1201 gcattaaaaa attagtggaa gacggttttt tacctcccgg tttaacaatt ggtccttcca
     1261 gcgtggattt gactctttca gattcattca gttggccaaa gccagataaa gacaaaataa
     1321 ttctgggcga atccgttccg cataagcagg tgcaaaaaga tgaatttgtt ttagaaccaa
     1381 atcattttgt actcgccagt accgcagaat ccatccgtgt tccccacgac atggcagctt
     1441 atgtggaggg acgttcaagt atcggacgct tgggattgca ggtacaaaat gcgggtttta
     1501 ttgatgcagg atttcacgga caaataactt tggaactcga aaatcaatcc ggatttccaa
     1561 ttgttttaaa aaaaggtgtc cggatttgtc agattgtctt tgtccaaatg agccgctctg
     1621 cagaacagcc gtacaacgga aagtattgtg gacaaagcgg agccactcca agtcgtctgg
     1681 aagtggatcc ggaatttaca taagcttatt tttgtcaggc ggatctacct gatttttggc
     1741 gcttgaactg tgttggttca ggctttttgt aaccgcagcc ctgaggctgt gcataattgg
     1801 taaggagatg tatggaaaag gtaaagttct ttgatacgac tctccgtgat ggagagcaat
     1861 cccccggatg ttcaatgtca gcagatgaaa agctgaccct tgccaaacag ctggagcgcc
     1921 tgggagtgaa tacaattgaa gccggatttg cagcatcgtc tccgggagac tttgagagcg
     1981 ttcgggaaat cggacggcaa ataacaaact caaccgttgt ttcactctgc cgtgcctcaa
     2041 aaaatgacat cgactctgca attgaagctt tgggtgatgc gaaaaactgg ggcattcata
     2101 ctttcatttc aaccagcgct ttgcacatga agcataagct gcagatggag cctaaagacg
     2161 tgaaagcaat ggcagtcgca gcagtggaac gtgcaaaaaa agctacggat aatgtagagt
     2221 tcagtcccga agacgcaaca cgcagtgatc cggaattttt ggttgaagta ttgtccgaga
     2281 cagtacgtgc aggtgctaca atcctgaaca ttccggacac ggttggttac accactccgg
     2341 atgaaatgtt tgacttaatc acacgtctcc ggaatgaagt ttacaatgcg gacaaggtta
     2401 ctttttctgt gcactgtcac aacgatttgg gattgggagt agcaaattcc ctggctgcgg
     2461 ttcgtgccgg agcaaggcaa atcgaatgta caatcaacgg catcggtgaa cgtgcaggaa
     2521 acgcgtcact tgaagaatta gtgatggcac taaaaacacg tcaggagtat tttggttttg
     2581 aaagcgacat tgtaacggag cagctttttc catccagcaa actgctttca aaaattaccg
     2641 gagtagatgt tcaaccaaat aaagcaattg tcggcgcaaa tgcctttgcg catgaggcag
     2701 gaattcacca gcacggagtt ctcaaaaatc cattgactta cgagatcatg accccacagt
     2761 cagtgggtat caagtccagt aatttggtga tgggtaagca ttctggacgc tttgcccttg
     2821 gagaacggat caaggaactt ggttttgacc tcaatgacat tgatctaaat catattttta
     2881 aagaattcaa acgacttgca gacctcaaaa aacaggtttt tgatgaagac attgaggcac
     2941 tggtaacaca tggaataggc aagcagacgg acaaatattc tttgga
//
LOCUS       DTVD01000038            2966 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13394_length_2966_cov_441.528684, whole genome
            shotgun sequence.
ACCESSION   DTVD01000038 DTVD01000000
VERSION     DTVD01000038.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13394_length_2966_cov_441.528684"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2591)
                     /locus_tag="EYO68_01345"
     CDS             complement(<1..2591)
                     /locus_tag="EYO68_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016952040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIC06479.1"
                     /translation="MTAKSSRQTEPQNMINARMYNRPIAIVGMSALFPDAPNLHQYWD
                     NIINKIDSIIDIPKSRWNIEDYYDIDSEAPDKTYCKRGGFIPDIDFNPMEFGIPPNIL
                     EVTDVTQLLGLLVAKSAMKDAGYGETSDEVLDATGVILGVTSGMKLLGSLTSRLQYPI
                     WEKVLRRSGISESDTGQIIEKMKKAYVRWEENSFPGLLGNVIAGRIANRLNLGGTNCT
                     VDAACASSLSAMKMAISDLLEYRSDMMIAGGVDADNSPMMYMCFSKTPAFTKGENPRP
                     FDEDSGGVMIGEGLGMVVLKRLEDAERDGDRIYAVVKGIGTSSDGKFKSIYAPRSGGQ
                     AKALRRAYEDANVEPLCIGMLEAHGTGTAAGDVAEFEGLKEVFTENKPEKSGYPDYQH
                     IALGSIKSQIGHTKAAAGIAGLIKTALALHHKILPPSINIDRPSKKLGIENTPFYLNT
                     EPRPWITDGEPRRAGVSAFGFGGTNFHYVLEEFQDVHSGPFRMHITSQTVFLSAATPK
                     ELDKACNNLLAELETEGAEQRHSQLLESSRENSIPKKHARLGFVTDSLENTVELLGQA
                     ISTLESKADKTEWNVKGIVYREKALETKGKVVALFSGQGSQYMNMAKELFFNFPAMAA
                     PFSALDKLFTGKNGKSAAAARPLSDLVFPIAVFEDEELEAQQAQLQLTENVQPAIGGI
                     SAGLLRIARDAGLQIDFAAGHSFGELTALWAAGVISEDDYYKLAYARGQAMAAPDDPD
                     FDAGSMLAVMGEVEKLEADLTEFPDVVMANLNSKKQVVLAGPTAAILLVQDALKAKKY
                     TVVKLPVSAAFHTPLVGHAQKPFAKAIRSVKFNKPQIPVYSNATAKAYPATAKTIQKQ
                     LESHILNS"
     gene            2891..2966
                     /locus_tag="EYO68_01350"
     tRNA            2891..2966
                     /locus_tag="EYO68_01350"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:2924..2926,aa:Asn,seq:gtt)
BASE COUNT          740 a          687 c          643 g          896 t
ORIGIN      
        1 gaattgagaa tatgtgattc cagttgtttt tgaattgttt ttgctgttgc aggatatgct
       61 tttgcagttg catttgaata aaccggaatt tgcggcttgt taaattttac tgagcggata
      121 gcttttgcaa atggtttttg tgcatgtccc acaagcggtg tgtgaaatgc tgcagatacg
      181 ggtagtttta caaccgtgta cttttttgct ttaagggcat cttgtaccag taaaatggct
      241 gcggttggtc ctgcaagcac aacctgtttt tttgagttca aatttgccat tactacatct
      301 ggaaactcag tgagatctgc ttcaagtttc tcaacctctc ccatgacggc caacatactt
      361 ccggcatcaa aatctggatc atccggagca gccattgcct gccccctggc gtaggccaat
      421 ttgtaataat catcttcgga aatcactcct gcggcccaaa gtgcagtcag ttcgccgaaa
      481 ctgtggccgg ccgcaaaatc tatttgaagt cctgcatcac gagctatccg gagtagtccg
      541 gcactgattc cgccaattgc aggctgcaca ttttcggtta actgcagctg tgcctgctgg
      601 gcttcaagtt cttcatcttc aaaaactgca attggaaata ctaaatcaga taagggcctg
      661 gcagcggcag cagactttcc gttttttcct gtaaaaagct tatccagtgc actaaaagga
      721 gctgccatgg caggaaagtt aaaaaacaac tccttagcca tgttcatata ttgagaaccc
      781 tgtccggaga aaagcgcgac tactttccct ttggtttcca acgccttttc gcgatatacg
      841 attcctttta cgttccactc tgttttgtca gctttacttt ccagtgtgga gatcgcctgc
      901 ccaagcagtt ccactgtgtt ttccagggaa tctgttacaa atcccaaccg tgcatgtttt
      961 ttgggaatcg aattttcccg tgaactttca agcagttgac tgtgacgctg ttctgctcct
     1021 tctgtctcca gttctgccag caaattgtta caggctttgt ccagttcttt gggagttgct
     1081 gcggacaaaa acacagtctg agatgttatg tgcatccgga aggggccgga atgaacgtcc
     1141 tggaattctt ctaaaacata atgaaaattt gttcctccaa aaccaaaagc actcacgccc
     1201 gcacgcctgg gttctccatc ggtgatccaa ggccgcggtt cagtattcag ataaaatgga
     1261 gtattttcta taccaagttt tttgctggga cgatcaatgt tgatggaagg aggaagaatt
     1321 ttgtggtgca gggcaagtgc tgttttgatc aaaccggcaa ttcccgcggc agcttttgtg
     1381 tgaccaattt gggatttaat actgccgaga gcaatatgct gataatctgg ataaccggat
     1441 ttttcaggtt tgttttcagt aaaaacctct tttaatcctt caaattctgc tacatcacct
     1501 gcagcagttc cagtaccgtg tgcctcaagc atcccaatgc agagaggttc cacatttgcg
     1561 tcttcataag ccctgcgtaa tgctttggcc tggcctccgg aacgtggagc ataaatactt
     1621 ttgaattttc cgtcgcttga ggttccaatc ccttttacaa ctgcataaat gcggtcaccg
     1681 tcccgctcgg catcttccaa acgtttgagg acaaccatac ccagtccttc acctatcatc
     1741 acgccgcctg aatcttcatc gaagggtctt ggattttcgc cctttgtgaa agcaggtgtt
     1801 ttactgaaac acatgtacat cataggagag ttgtccgcat caacgccgcc ggcaatcatc
     1861 atgtctgagc gatattccag caaatcgctg atagccattt tcatggcact tagtgaactc
     1921 gcacaggccg catcaaccgt acagtttgta ccacccaggt tcaaacggtt ggcaatgcgt
     1981 ccggcaataa cgtttcccag caaaccggga aatgaatttt cctcccagcg aacataggct
     2041 tttttcatct tttcaattat ttgccctgtg tcactttctg aaattccgct gcgccgcaaa
     2101 actttttccc agattggata ctgaagccgt gaagtcagag agccaagtag cttcattccg
     2161 gatgttacac caagtatcac gccagttgcg tcaagtacct cgtcacttgt ttctccataa
     2221 ccggcgtcct tcatcgcaga ttttgcaacc agcagtccca gcagttgagt aacatctgtc
     2281 acttccagaa tgtttggtgg aattccaaac tccattggat taaaatcaat gtccgggata
     2341 aaaccgccac gcttgcagta cgttttgtct ggagcttcag aatcaatatc gtaataatct
     2401 tcaatgttcc agcgtgactt tggaatatca atgatgctgt cgattttatt gatgatgtta
     2461 tcccagtact gatgcaaatt tggagcatcg ggaaacaatg cagacatgcc gacaatcgca
     2521 attgggcggt tgtacattct ggcattaatc atgttttgtg gttctgtctg gcgggaggat
     2581 ttggcggtca ttcttttttc agtgctggtg actttctaat gtgttccggg ctgtgtatcc
     2641 gcaaagtatt ttggtcccag tatctaaaaa atagcaggaa gccttgacgc aggagtttat
     2701 aacaccttca agcttcatgc cgaatatagc ataaataaac tgtataagtc tatgatttta
     2761 acgttaaagg gctttctgtt gcaagttctt cctgattaga gctgggttat aattatgttt
     2821 cagtaaaaat tccttgaaat tctaaacaaa atcgtttaac tttaacttac caaattaaag
     2881 ctgattatgt tccccaatag ctcagttggt agagcagatg actgttaatc atcgggtcgc
     2941 tggttcgagt ccggcttggg gagcca
//
LOCUS       DTVD01000039            2941 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13591_length_2941_cov_313.729383, whole genome
            shotgun sequence.
ACCESSION   DTVD01000039 DTVD01000000
VERSION     DTVD01000039.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13591_length_2941_cov_313.729383"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            105..848
                     /locus_tag="EYO68_01355"
     CDS             105..848
                     /locus_tag="EYO68_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC06480.1"
                     /translation="MAGRLKDKKSLITAAGKGIGRSTVLAFVNEGARVLATDIDQDSL
                     DSLKKECPEIETRLLDVTNPEEIQAIAKETGAIDVLFNCAGFVHHGTLLDCEESDWEF
                     SFDLNVRSMYRMIRAFLPAMLKTGGGSIVNMSSVASSVKGLPNRFVYGASKAAVVGLT
                     KSVAMDFIKQGIRCNAICPGTVESPSLQERIKAQGGNFEDVMAAFVARQPIGRIGTPE
                     EIAALVVYLASDESAYTTGVAWSINGGMT"
     gene            879..1745
                     /locus_tag="EYO68_01360"
     CDS             879..1745
                     /locus_tag="EYO68_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HIC06481.1"
                     /translation="MKLLRYETSTSGTSGGQEKPGLLDEAGVLRDLSGIVDDIAGETL
                     LPENIERLRNTDPASLPEVEGNPRLGPCVGQVGKFICIGLNYSDHAKETGMSIPPEPI
                     IFAKATSAICGPNDDVIIPRNSVKTDWEVELGVVIGKPAKYVDEASALDHVAGYCVIN
                     DVSEREFQIERSGQWVKGKSCDTFGPTGPWLVTPDEVGDPQNLDLWLEVDGKRYQDGN
                     TRTMVYGVAHLIHYLSQFFTLHSGDIISTGTPPGVGMGQKPEPIYLRAGQTMQLGIEK
                     LGVQNQCVVADK"
     gene            complement(1848..>2941)
                     /locus_tag="EYO68_01365"
     CDS             complement(1848..>2941)
                     /locus_tag="EYO68_01365"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC06482.1"
                     /translation="SGYAPDMPTMIVGRLLQGMGGGGLVSIAFIGASTLFPKKLWVRI
                     IAVISGVWGVSSLCGPLIGGYFVSGGNWRGAFWAFAAQGLVAVLVAPPLLRRQFQTQK
                     EQNQRMPLARLCLLSVAVLAICQAGVMTSTGVASVLCAGGVLLLLAFLKLDADSENRI
                     FPQGVLNPRTVSGSGLMMMSALFMATIALTVYGPLLMYIIFGAEPLVAGYIIALESLG
                     WTVVAISTSGVSGRIEPRLIRLGSVLVSISMVGLIYAMPSGPLWLIALLSTIMGMGYG
                     MSWSFVSKRIIANVSEAERTQASASIPTFMRVSMALGSALSGIIANFSGFSEDSSVEV
                     AQNVAFWCFAAFVPLILVGLFTAWRVSRE"
BASE COUNT          810 a          717 c          748 g          666 t
ORIGIN      
        1 attggagcag ttttgtagtc ttaattattt ttattcatag attattgaag aacaaaacag
       61 atataattct gtagttttca ttattatttt gaaaggaaga aattatggcg ggaagactta
      121 aagacaaaaa atcattgatc acggcagcag gcaaaggaat aggacgctct acagtacttg
      181 cttttgtcaa cgaaggcgcc cgggttttgg caacagatat cgatcaggac agcctcgatt
      241 cactcaagaa agaatgtccg gagattgaaa ctcgtttgct tgatgttacc aatcctgaag
      301 aaattcaagc aatagcaaag gaaacaggag ccatcgacgt gctttttaac tgtgccggat
      361 tcgtgcatca tggaactttg ttggattgcg aagagagcga ctgggaattt tcctttgatt
      421 taaatgtacg ctcaatgtac cgcatgattc gggcatttct accggcaatg ctgaaaacag
      481 gaggtggaag cattgtcaac atgtcttcgg tagcctcgag cgtaaaaggt ttgccgaacc
      541 gttttgttta cggtgccagc aaagcagctg tagttggctt gacaaaatct gtggcaatgg
      601 attttattaa acagggtatt cgctgtaatg ccatctgccc cggaacggtg gaatctccat
      661 ctctgcagga acgtattaaa gcgcaaggtg ggaatttcga agatgtgatg gcggcttttg
      721 ttgctcgcca accaattgga agaatcggta cgccggaaga gattgctgcc ttagtcgttt
      781 atctggcctc agatgaatca gcctacacaa ccggtgtggc atggagtatc aatggtggaa
      841 tgacctaaaa gttaacttaa cctttattta aaacgacaat gaaattactt agatatgaaa
      901 cttctacctc aggaacttcc ggagggcaag aaaaacccgg actgcttgat gaagctgggg
      961 ttctgcgtga cctttccgga atagtcgatg acattgccgg tgaaacgcta cttcctgaaa
     1021 acattgaacg tctgcgaaac actgaccccg catcgcttcc tgaagtggaa ggcaatccgc
     1081 gtctcggccc atgcgtaggg caggttggca agttcatttg cattggtctc aattattccg
     1141 atcatgcgaa agaaacaggg atgtccattc cgccggaacc gattattttc gctaaggcca
     1201 ccagcgcaat ctgcggtccg aatgatgacg ttattattcc acgcaattca gtaaaaacag
     1261 actgggaagt cgaacttgga gtagtcatcg ggaaaccggc aaaatatgtt gatgaagctt
     1321 cagctcttga tcatgttgct ggctactgcg ttatcaatga tgtctccgaa cgtgaattcc
     1381 agatagaacg ctcaggtcag tgggtcaagg gaaagagctg tgacactttc gggcctaccg
     1441 ggccctggct ggtgactccg gacgaggtgg gggacccgca gaatttggat ctctggctgg
     1501 aagtcgacgg gaagcgctac caggacggca atacacgcac aatggtttac ggtgtcgcac
     1561 atctgattca ttacctgagc cagtttttta cgctacattc cggagatatt atttctaccg
     1621 gaacaccgcc cggagttggc atgggacaaa aacctgaacc aatctacctt cgcgcaggtc
     1681 aaacgatgca gcttggtatt gaaaaattgg gtgttcagaa tcaatgcgtt gtggctgata
     1741 agtaagttta caaacaattt ttataatttg ggattctgtg gggttttgga taaattatta
     1801 aaaggtagga aagaaaaggc tgaatcaaca gcgaaaacag aactccttca ctctcggctg
     1861 acgcgccagg cggtgaagag accgaccagg atcaatggga caaaagcggc gaaacaccag
     1921 aacgcaacgt tctgcgcaac ttccacgctg ctgtcttcgg aaaatccgga gaagttggca
     1981 atgatgccgc tgagggccga tcccaacgcc atcgacaccc gcatgaacgt gggaatggac
     2041 gctgatgcct gggtacgctc cgcttccgaa acgttggcga tgatgcgctt ggacacgaag
     2101 ctccaggaca tgccataacc catgcccatt atggtcgaaa gcagggcgat taaccagagc
     2161 ggacctgaag gcatagcata gatcaagccc accatcgaaa tgctcaccaa gaccgacccc
     2221 agccggatca ggcgcggttc aattcgccca ctgacaccgg acgtgctgat cgccacgacg
     2281 gtccagccca gtgactccaa ggcaatgata taaccagcta ccaaaggctc tgccccaaaa
     2341 ataatataca tgaggagggg gccatagacc gtcagcgcga tggttgccat gaagagagcc
     2401 gacatcatca tcaggccgga tccggagacc gtccgggggt tgaggacacc ttgcggaaaa
     2461 atacggttct cgctgtccgc atccagcttg agaaaagcca gcagcagaag gacacctcct
     2521 gcacataaga cgcttgcgac cccggtggag gtcatcaccc cggcttgaca gattgccagc
     2581 accgcgacgc ttaaaaggca aagccgtgcc aagggcatcc tctggttctg ttccttttgt
     2641 gtttgaaact gccttcttaa aagcggtggg gccaccagca ctgctaccaa tccctgcgcc
     2701 gcaaacgccc aaaacgctcc gcgccagttt cccccggata caaaataccc tccgatcaac
     2761 gggccgcaca aggaagacac cccccagact ccggaaatca ccgcgataat ccgtacccac
     2821 agttttttcg gaaacagtgt ggatgcgccg atgaacgcaa ttgacaccaa tccgcctcct
     2881 cccatgccct gcaaaagtcg accgacgatc atggtgggca tgtccggagc ataaccgctg
     2941 a
//
LOCUS       DTVD01000040           34172 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_137_length_34172_cov_297.861887, whole genome
            shotgun sequence.
ACCESSION   DTVD01000040 DTVD01000000
VERSION     DTVD01000040.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 34172)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34172)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34172
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_34172_cov_297.861887"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            341..1750
                     /locus_tag="EYO68_01370"
     CDS             341..1750
                     /locus_tag="EYO68_01370"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIC06483.1"
                     /translation="MLNNSQKSNRQVDVAIIGAGSAGLPAFRAARKHSEKVVLIEGGV
                     YGTTCARVGCMPSKLLIAAAEAAHSVETASGFGIQTAKPVVNGREVMERIRGERDRFV
                     GFVLDDIENIDQKHHLRNHAKFLDDHRLEVGEEIIEAKSVVIATGSSPKLMPMFKGLG
                     DRLIVNDDVFEWETLPESVAVFGPGLIGIELGQALHRLGVRIRVFGRSGSLTPLSDMA
                     IRDYAEKVFQAEFPLDTKANLHGLEREGDQVKIRFVDREDGKEKEENFEYVLAATGRS
                     PNVINLGLENTSLELDSREVPVFDPNTMQCGDSSIFIAGDVNNTMLLLHEAADEGRIA
                     GDNAGRFPDIKSGMRRSPISVVFCDPQIAMVGQSWGELKGQENVVVGKLSFEGQGRSR
                     VMLKNKGLMHIYADRTSGLFLGSEFIGPQAEHLAHLLSWAHQQKMTIPQMLEMPFYHP
                     VIEEGLRTALRDTNVQLSL"
     gene            1810..2565
                     /locus_tag="EYO68_01375"
     CDS             1810..2565
                     /locus_tag="EYO68_01375"
                     /inference="COORDINATES: protein motif:HMM:PF01494.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIC06484.1"
                     /translation="MSEHNYDVIIIGGGPARLFAGIACVRNELRTLVLEKQTYPIDKA
                     CGEGIMPSGVNNFKKLGVAPFLEKYPYHLFKGIRYVSAKGSVAEANFAEGCGWGVQRT
                     KLSAALLECAETLPNLEIQEGASVACSLEENPEVSIKNNVFHPKLLIGADGLHSRVRK
                     LANLEATIPKLKRWGVRQHFNVEPWSAFVEIHWGSGLEAYVTPVSETSINVSLLWDAK
                     RYKPEHGGKKLFCSMLNEFPELPGSLKKRRCSG"
     gene            2513..2929
                     /locus_tag="EYO68_01380"
     CDS             2513..2929
                     /locus_tag="EYO68_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06485.1"
                     /translation="MNFRNCQDRLKNADVLDEPVAKGPLFQKTLGVANGKILLIGDAA
                     GFFDPITGEGIDFAARQALLLEKYEEPVLKENSGNLVKAMFDYSRASAQIYRRYQIMT
                     SLVLLLRLWPKLTDGVIQVLHSFPALFQKLLSVNMR"
     gene            3024..4271
                     /locus_tag="EYO68_01385"
     CDS             3024..4271
                     /locus_tag="EYO68_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIC06486.1"
                     /translation="METKKRVVVTGRGVISPIGSSVSDYWEGLCNGVSGVRKITEFDS
                     SGASCQIAGMVTDFDSDVHFSHREKKRYERFTQFGLVAALRAAEEANLLDQDGDSGEI
                     GVMMGTGVGGITSYAQAYDALYAGPEERPIDAYTIPRIMNSAVSSAIAIRLGIKGPNF
                     TLNTACSSGGNAVGQAMQWIREGRLRAVICGGAEAPVTYGLLRAWDALGVLSRKYNET
                     PETACRPFDKNRSGFVLAEGAAVLVLESLESANKRNAPVFGEIAGFGSNCDASSLTNP
                     DENGVAESMKLALDDAEMSPDSIGYINAHGTGTRLNDPLECRAIHRVFGDRSVKLPVS
                     SSKSLFGHAMGASSALEALATLLAVQNDCAPPTLNCDDLDPECVIDPVPYNSRKVQIE
                     AALSNSFAFGGSNAVLVFRKWKP"
     gene            4259..4513
                     /locus_tag="EYO68_01390"
     CDS             4259..4513
                     /locus_tag="EYO68_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIC06487.1"
                     /translation="METITRKKIEQSCLQTIAVEMGLKSNDLNTEMNLRDDLDIVSLD
                     AMNIIMALEDEFKIEADIETVLEMQKVSDIIDHLEIRINS"
     gene            4598..5764
                     /locus_tag="EYO68_01395"
     CDS             4598..5764
                     /locus_tag="EYO68_01395"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02797.13,HMM:PF08392.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HIC06488.1"
                     /translation="MPHIINTATAFPTHYHSQQEISFALRAVWIKKGLDVAIFDRLQK
                     AVTVEGRYLALPMSEYYKLDGFADCNRAWLEVALELGERVINDLLEKTNLHPQEIQML
                     MSVTSTGVTVPSLEARLMNRIAFAQDLKRVPLFGLGCLAGTAGVARVGDYLKGHPEEA
                     AILLTIELCSLTLQAEDLSIANIISSGLFGDGAAAVLLVGDQHPLVKSNEKRNSKANE
                     FHGSKVSKGSKDESERDHEFHKPRVLSSKSIFFPDSEEVMGWDVTDRGLKIMLGQGVP
                     GYAKQLRPHVDSFLAEHGLSIPDIALWLTHPGGPKVIDTIESCLELPPKSLDKTRKHL
                     REVGNISSTSVLVILDECLNGPVPKIYGKSDTGNFGMMISLGPAFSGELVLLQW"
     gene            5758..6273
                     /locus_tag="EYO68_01400"
     CDS             5758..6273
                     /locus_tag="EYO68_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06489.1"
                     /translation="MVSLWGFMLLVALLAAQRLWELRRSRRNEIRMREKGGFERFPEH
                     YRVLVALHVSWFAAMLAEVWYFRTAPPLWLVIAAFSGLAAGQTLRYFAIRTLAERWST
                     RIYVVPDAPLIKHGIYRFVRHPNYLGVILEIASVPLLHAAYGTAVVFSLANLIILRHR
                     IRLEELALDEI"
     gene            6368..7783
                     /locus_tag="EYO68_01405"
     CDS             6368..7783
                     /locus_tag="EYO68_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIC06490.1"
                     /translation="MTLSLHFDKGTIQLHGMAGRYMQHLDGISWDERTNSYRTPAANY
                     RKLVTALCEKNISFQDHARKFSAETFVLKKNIRPRSFQSEAAEAWLSEKHGVVTLPTG
                     AGKTILAVMLIAETGRPTLIHVPTIDLMRQWKEVLSEYFETPIGLLGGGINDIQTITV
                     ATYDSALIHVTHQGNLFGLLVFDECHHLPGEQNQFTALGSIAPFRLGLTATPERADGK
                     EQVLYELCGPLCYEVHIDELSGRTLAPYVVETIEVDMRPEEREQYEISRECYTDFLRK
                     ARISFQHPSGWAIFLRRTSESPEGRKAFKAYLLQKKLSQASGAKVDRLWELIQQHKGD
                     RMLVFTQDNEMAYRIGNNFLLPVLTHHTKLPEREAFLKSFRSGEYPILVTSKVLNEGV
                     DVPDANVGMIVSGSGSIREHVQRLGRILRARPGKQAILYELVSKDTGEYFTNQRRRKH
                     RAYEGPVVLPDESGQNSAQIN"
     gene            7659..8993
                     /locus_tag="EYO68_01410"
     CDS             7659..8993
                     /locus_tag="EYO68_01410"
                     /inference="COORDINATES: protein motif:HMM:PF05626.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HIC06491.1"
                     /translation="MNLFPRIPENILPTSDVENTVLTKDLLCFRTNRGKILPKLINPE
                     ATKLLEIAEELLAVFSGSVGQVREKLEEETKQVLDGFPGNAIVGRGLEKLLLDRTEFD
                     TEAKTELTDLREKVFFNSAAFFSAKKAFRFPGVEMGDKADLGHYQIEIAKSVGISIEQ
                     LGRQLYGDLPPFQQVLRFRKMTAAGLLHRYNCAQLQGLLLRCEGLNVSLPESKAAGLR
                     QLLKYLRFNKLLAKISHDQRKKKTLMLEIDGPLSLFVNTQKYGLNLANFFPAILQQPE
                     WELDAEVRIRKNHVHSLHLDQSCGIRSHYRQFLAYIPDEIQLFSQQLAKQIPDWELTS
                     SADFIPLTGESLCFPDFLLKHHSGKKVSMELFHKWHVAPLQSRLEQLDSQKGAPLLIG
                     INRSLLNNIQLAEQVEASTYFSRYGFFFREAPTITKLRPLLDSWLSNVQKTI"
     gene            9478..10806
                     /gene="tig"
                     /locus_tag="EYO68_01415"
     CDS             9478..10806
                     /gene="tig"
                     /locus_tag="EYO68_01415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIC06492.1"
                     /translation="MNSTVEELGKLKYSLALEVSLDEIKPTYNAIYRELKKTRLNGFR
                     PGKHPKGWLDKRFKVAMQQEAVDRVIPGYMDDALQEHSLRPATMPIIQKIDFDLKSPL
                     SAILHFEIAPELPKLNYEKIVLERKDIEAVSAKNISEELEGLIQREEVLVPKVGEDVK
                     VEKDDWVLINYEGTIEDEKFTDSTATEMQFKIGSSDLEEFHSGLMNMSSGEEKDVELA
                     LPERFGENAGKKAIFKIYLTEISAVKRPEMDEDFFKKFGVADEDELKEKVSENIKSRK
                     TAELQSEYRIAVRAQLSDLYDDFNLPEELVKYGQEQVERELEQASSEKEIPEEEKEKR
                     RQEGIENAKMDLRMKFILDSIGEHEEMKFDKNEAAREFVGLAQITGQSPDELIKSPFG
                     HDMYERIVVRKKGDATLDRVVARVFGDPIEEFAAEDHEHVHDENCEHDHS"
     gene            10992..11588
                     /gene="clpP"
                     /locus_tag="EYO68_01420"
     CDS             10992..11588
                     /gene="clpP"
                     /locus_tag="EYO68_01420"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIC06493.1"
                     /translation="MSLIPMVVEQDSRGERAYDIYSRLLKDRIIFLGSPVDDNVANVM
                     IAQLLFLEAEDPESDISLYLNCPGGLVTSGMAIYDTIQYLKPDVQTICIGQATSMGAV
                     LLASGASGKRQALPHARIMIHQMMGGFSGQAEDIDIQAKEIIRMTDVLNDVLARHTGH
                     KIKKIAQDTQRDYYFSSQEAKEYGIIDEVMQNRKLKAL"
     gene            11602..12855
                     /gene="clpX"
                     /locus_tag="EYO68_01425"
     CDS             11602..12855
                     /gene="clpX"
                     /locus_tag="EYO68_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIC06494.1"
                     /translation="MNSVKEALLRCSFCGKTQEEVKKIIAGPTVYICDECIDLCNEIM
                     EEEYQNEHADEFQKELLKPAEIKDVLDNYVVGQDRPKKVLAVAVHNHYKRIKANFDKS
                     EVELQKSNILLIGPTGTGKTLLAQTLARILNVPFAVADATSLTEAGYVGEDVENIVLK
                     LLQAADYDIERAEQGIIYIDEIDKITRKSENVSITRDVSGEGVQQALLKIIEGTVANI
                     PPQGGRKHPHQETMQVNTENVLFICGGAFTALDDIIRRRIGRNAIGFGADVNKKTRNV
                     EVLSQLEPEDLLKYGLIPEFVGRLPVVATLHDLEVDHLVQILKEPKNALIRQYEKMFE
                     LDNVKLTFNKEAIHTLAKEAIKRKTGARGLRSIIENSMLDLMYEIPSDPDIKEVEINK
                     DSILQKAEPKLTRNPASSIPRAKAS"
     gene            12906..13904
                     /locus_tag="EYO68_01430"
     CDS             12906..13904
                     /locus_tag="EYO68_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HIC06495.1"
                     /translation="MENLDVYGVCNPLIDLLSHVPDSFLTERGLNKNSMNLITLEEQQ
                     TFLLALAAGKISVEFAAGGSGANTMIGIAQLGGRTAFSGKIGRDEHGKIYREKLEDLG
                     VNAVLGEGDGTTGSSLILVSEDGSRAMNTYLGMSQELQVPDIDPEVIQASKYLYLTGY
                     LWDTEVQKKTVINALDEAKKREIKVALSLSDPFCVNRHKDDFKKLLQGYVSMVFCNQE
                     EAFSLLDTTVTQEAVEILSQWTETVVLTLGSNGALISHLGQTFYIDPSQICVEDTTGA
                     GDAFAAGFLYGMTHEFSPLESGRIGAVLAGAVIEQTGPRYQGHAQTLIRENIGIKL"
     gene            13946..15184
                     /locus_tag="EYO68_01435"
     CDS             13946..15184
                     /locus_tag="EYO68_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIC06496.1"
                     /translation="MELLAPAGNLDKLKTAVLYGADAVYLAGPKFSLRGASDNFSDTE
                     LCEGIKFAHLNNCKTYVTLNAFLHDKELRELPEYVRFLEKTSVDAVIVSDLGVLTVVQ
                     QHSKLPVHLSTQASCLNIHSAKVWKNLGVKRLVLGREVSLEEAGCIRKEVDIEIELFI
                     HGAMCMAYSGNCTISNYTAGRDSNRGGCIQSCRFSYSAIPVNNAVAVNSTDNTPSSLM
                     SSKDLRGIDLLPKFLKIGVDSIKVEGRMKSPLYAATTTSAYALALKWCNSTVQEQWSE
                     KLQELSGMLEKIPHRGYTDGSLNNEADAESVYQGERNGRNSGYEIAGTVMEVEDGKSF
                     TMLTHNSFDRNKTLEILTFDGGVIKISTQEMKDLNNKPVKRANPSRLFRFACSEAAEQ
                     SADVSGISQVQPLNVVRLKI"
     gene            complement(15229..16488)
                     /locus_tag="EYO68_01440"
     CDS             complement(15229..16488)
                     /locus_tag="EYO68_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIC06497.1"
                     /translation="MLVEYGSVFMILAVVLGFYMSWGIGANDVANAMGTSVGSGAISV
                     KQAIIIAAIFEFSGAFLAGGHVTKTIRKSIIDPTPIMDQPEILVWGMLSALLAAALWL
                     TFASWMGWPVSTTHSIIGAIVGFAVVGIGVDAVNWSKIGTIVLSWIVSPLVGGTLAYL
                     LMRSIQKFILDSDTPFLNAKRYAPFYVFLVGFLLSLVTLFKGLKHLDITFSVGQSFFA
                     AVIFGVIVAVIVRFMINRLKMKEAESIHDQFTQIEKIFGIMMIFTACAMAFAHGSNDV
                     ANGIGPMAAIVSIVESGGEMAQKSNLPLWILLTGAVGIVLGLSTLGYRVMQTIGSKIT
                     ELVPTRGFSAELAAAATVVIASRTGIPVSTTQIAVGAVMGVGLARGIGALDLRVVGGI
                     FMSWVITLPVGALLSIFFFFFFRGVFL"
     gene            complement(16529..17203)
                     /locus_tag="EYO68_01445"
     CDS             complement(16529..17203)
                     /locus_tag="EYO68_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HIC06498.1"
                     /translation="MINLSSMFAKSPFKPLRDHMDKVVESVAPLKDFFDALHQGNYSK
                     VEEIQQQISLAEEEADIIKNEVRNHLPRSIFMPINRRDLLEMLDMQDTIADVTQDIVN
                     LLTLRRMCLPTDLCQELIQFVEKSQQVCYMAQGLSQEFGDVLESGFGRHEIDKLLKMI
                     DEVSNAETEADSLEDALVRRIFDIEDSMKAVDVVFWYKIFELIGDIADYSKKTGNRLR
                     LMIASN"
     gene            complement(17291..18382)
                     /gene="gmd"
                     /locus_tag="EYO68_01450"
     CDS             complement(17291..18382)
                     /gene="gmd"
                     /locus_tag="EYO68_01450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIC06499.1"
                     /translation="MGKVAFITGITGQDGAYLAELLLKKGYIVHGLKRRSSSFNTGRI
                     DHLYQDPHVSKRNFILHHGDLTDSTNLIRIIQEIQPDEIYNLAAQSHVHVSFETPEYT
                     ANADGLGTLRILEAIKLLGMVEKTKFYQASTSELYGIAQEVPQNENTPFYPRSPYAVA
                     KLYGYWITVNYREAYNMYACNGILFNHESPVRGETFVTRKITRAAVRISMGTQEQVYL
                     GNLDAKRDWGHAGDFVDGMWRMLQQEKPEDFVLATGVTTTIREFAERAFAEVGITLGW
                     SGRGVDEVGRDTKTGNTVVSVDPTYFRPTEVDLLIGDATKARKKLGWKTTLDLQQMIE
                     EMIVADLEEARKDQHLQSGGFKTNGVNED"
     gene            complement(18419..19822)
                     /locus_tag="EYO68_01455"
     CDS             complement(18419..19822)
                     /locus_tag="EYO68_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIC06500.1"
                     /translation="MANRERLLNVGSTVSKKFSATETAEAELKKTSTEKESELKLNNN
                     PKAADLLATAQKVFLPTYAPSLILTHSKGALIWDSDGNEYIDLGSGISVNSLGHLDSD
                     LLQALNIQAGKIWHTTNLYLTEPAIKLAEELVTATFADRVFFCNSGAEANEAAIKIAR
                     KYSSLSQPAGKREILTFEGSFHGRTLATVTATAQPKYQQGFEPLPGGFRYCPFNDFDT
                     VTEMIGPQTCAVLIEPIQGEGGVNPVKPGFLSHLRKLCDQHDVLLIFDEIQCGMGRTG
                     KLFGYQWENSEDSEQPDNSVTNLKPDIVTMAKALGGGLPIGAMLCTEKVAQTFKPGDH
                     GTTFGGNPIVTAVASAAFRKVNTPQMMEQVQKKGLVIKNNLEALNDELSLFETIRGRG
                     MMLGAVLSKAWKGKASTMVTACQEEGVLVLVAGPDVLRFLPPLNISTADLQTALDRVG
                     KALKNLHADELSSSTKT"
     gene            20484..23132
                     /locus_tag="EYO68_01460"
     CDS             20484..23132
                     /locus_tag="EYO68_01460"
                     /inference="COORDINATES: protein motif:HMM:PF02460.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HIC06501.1"
                     /translation="MTPLKIIQGWVVRYPKRILFLTLFLGASVVPSLLFLKNDPSPHL
                     LPVSHPARQALQQLREDFTGTNSGVFIMLEAKDTIFKTNTLERIQRLTEAIQNMQLLS
                     TEDLEALNVIAEQMSGKEGLRLQKLLPKEVKDLNDMFWMEFEEMRETLENEGRWFPEW
                     NSLIRNIEVRSAPVVEVTSISNTDDIYGSEEGLTIEKIFEVIPGTPGEMKSLRRRVLE
                     NDLFRNSFFSEDGRRTGIFVELAMDEDDSEILYSTYIALERIFEENPGIDKHYIAGFP
                     IVAATLRTVIDQDTQRFFPFVALLAVFFLWLTFRRPSGVAVPMLVVGFSILFTLAIMV
                     VFEVPLNTITSALPVFLISIGVADGIHMFSEYRENRIEGLPREKAVCLMLDKLALPVT
                     MTSITTAVGFLSLTVSDIVPILTFGIFVAVGTLLAMVLSLIFIPALLMVLPEKVSASQ
                     EGSGTDENLSSGVHQVNFMDRLFQKSLDVMTAWVLRNARPVLLAALAICVISIYGLSQ
                     VKVESSLESYFQADAPFVIANRAMEKMSGSRTINIVITKTGEEEPWKNPDNLKVVEEF
                     QEFLANEPRVKRTLSLVDLIKRISYAFNENRKEFNRLPNGFEFLESEEIFEENGQTEK
                     RTVKREISGRDLIAQYLVLYENSGGDVLSDVVDSEYLNLNLAVTISSNSTTEEEKLLQ
                     SIEDYAVRQFPQGFSMTSSGMVPINVATSNEVVTSQIRSLSGSILAVFLMLALIFRSV
                     SRGLMGMIPLVFTVLFNFGVMGFFGVHLDIGTALVSSIVIGIGVDYSIHYLSSMFHEL
                     ADGTELHDAISNTVRRSGKAITSNAVTVGFGFLALSVSEFLPLVTLSWMIWLTLNISA
                     LATMILIPALAVTLTDVLKLVPSQND"
     gene            23375..24385
                     /locus_tag="EYO68_01465"
     CDS             23375..24385
                     /locus_tag="EYO68_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC06502.1"
                     /translation="MSNSLRIFHQLQADLNQIILGQERLIERLLIVLLADGHLLVEGA
                     PGLAKTRAIKALGEKIEGEFQRIQFTPDLLPSDVTGTDIYRPQEGSFHFQSGPVFHNL
                     VLADEINRAPAKVQSALLEAMGERQVTVGGQTYPLPDLFLVMATQNPIEQEGTYPLPE
                     AQLDRFLLHVFIDYPSIENELKIMRLVRKEALSEIQISSDKSPKSAVDSGTKSAGRKI
                     SAVHIFEARNEALNIYMAPEIERYLVEIIAATRIPEKYSDELARLITWGASPRGALSL
                     DRCARARAWLHERDFVTPEDLQDLSGDVLRHRIIESYEAESDGITTDDVIAKILAQVA
                     VP"
     gene            24633..26537
                     /locus_tag="EYO68_01470"
     CDS             24633..26537
                     /locus_tag="EYO68_01470"
                     /inference="COORDINATES: protein motif:HMM:PF00884.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIC06503.1"
                     /translation="MKYLDPTAVVLFFVVSLAGIVQHSFLLQLNSDWSERLFQAYALG
                     VGYDVMNGAIFSTLAVISPFPILFRKIFFSILGLGLFAFLFTDYHYVLIFGTHLPFST
                     IEYLNDSGAFLSSAAHAVNDITFWILFVFPSVMLVFMLWRFGKKRNSWKEDFKCRAST
                     LLFIILIGGPAASYSNSYVSKNMENPLTSAALQYFYYSKDREPDKKILRPEKSLKIVE
                     TLIQGKKPSGKIWDNYPLVRIREATGCTGQNHSELTKILCTTADRPNILILFLESFRA
                     AEVGVYGSKLGLTPEFDRWSKHGILYKNFFANGFQTRHGQVATYCSLFPNYGAAVMKR
                     YTGNSFMCLPEYLKNSGYYTSWVYGSDANFDGQSTFLPKVGFDKIVDEFDFPNEAVTL
                     GWGLSDEDLFRKWETVLDSEPEPFFSSALTTTNHHPFEVPDNYRLQAGDSVTDRYRES
                     LHYTDAMLGGFLKRISKKPWFKNTIIFVTADTSSYQNAEQHTNNYEEFVNLHSQVPLL
                     ILSGKSTALGKKSEGIIITQHASQVDLAPTILDILRKKYIVPWVGRSLLNSVDLLTDV
                     PQRAFTNRPGAYWAVIEEKSRYYRENDQRDHFFGDQDNQKGLHLKEIGSSWIETIHWV
                     LQENRVWPEK"
     gene            26542..27459
                     /locus_tag="EYO68_01475"
     CDS             26542..27459
                     /locus_tag="EYO68_01475"
                     /inference="COORDINATES: protein motif:HMM:PF01882.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIC06504.1"
                     /translation="MKDAAEKNGIFLGKMELPELVRIAHKKGLLLTAENVGSLGGPYL
                     TKLKGRGMEFAEARSYQYGDDVRHIDWRVTARTGTTHTKLFREERERPVMLVLDLSRR
                     MFFGTRQRLKSVQAARIAALTGWRALLRGDRVGGVIYSEEMHREFKPRSDRRHFLRLL
                     SNIMTEHNRIADRMNNNEWSFGSNSLFAQALRRVRQVVHPGSLIYIFSDFSGFNAECR
                     KHLFQLSKQNQLAAFLISDPLEKEAPSSGRYAMSDGEAVAWVNSENSKTRQLYASTYE
                     DQLKSLKHELQLCRLSLKMLSTVDNVSQI"
     gene            27493..27975
                     /locus_tag="EYO68_01480"
     CDS             27493..27975
                     /locus_tag="EYO68_01480"
                     /inference="COORDINATES: protein motif:HMM:PF14316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HIC06505.1"
                     /translation="MPEENPLINLKDIHLPPAVAIWPPAPGWWIVAVVLLLIFVFCGM
                     WISRRFERRKPKTEALRLLKNLQNQQNNTEESLEILRDLSQLLRRVALTFCADENVAS
                     LHGSEWLEFLDRTGKTTEFTQGAGHVFGKELYQQNPEIEIDVLFPMVKKWIMDCSQQH
                     "
     gene            28011..28988
                     /locus_tag="EYO68_01485"
     CDS             28011..28988
                     /locus_tag="EYO68_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIC06506.1"
                     /translation="MFEFIWLWVAWFWPLPLLFRVLPPVDRSVQALRVPFFHRIAVFP
                     KSGISAGKNSKVLLLAGLVWSLIVLAALRPQWVGDPVELPVSGRSVMLAVDLSGSMKE
                     TDLELHGESVTRLAVVKSVLRPFIERRKGDRLGLILFGDQAYLQTPLTFDRKTLRQML
                     EESELGLAGQRTAIGNAIGLAVKRMKDLPDKEKVLVLLTDGQNTAGEVTPEDAGRLAK
                     ETGLRIHTIGIGADEAWIRAFFGKQKINPSAELDEAMLKSLASQTGGTYFRARSTEEL
                     EKIYAMIDKIEPLEIEKEVFRPRQALFMWPLGFAVLIVGIWSAVLLIRN"
     gene            29425..31194
                     /locus_tag="EYO68_01490"
     CDS             29425..31194
                     /locus_tag="EYO68_01490"
                     /inference="COORDINATES: protein motif:HMM:PF13519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIC06507.1"
                     /translation="MVFRFLGRWWSKGNWEDVVEPHLLSFLATTPATQKRSLLPWIIC
                     LVGTILLLALSGPVWQKNLQPLLQKSLARVLVLDLSYSMLATDIKPTRFERARFKLKD
                     LLGHFVEGETALIVYAGDAFVISPLTHDPVTIASLLTGLHPNIMPLPGSRSDLAIELA
                     ADLLSRRNGGTGHIIWVTDGIEVQDIPAAENAVGLNRLSILAVGTESGSPIPLSGGGF
                     LKDKKGGIVIPKLYLKPLQKLAAQTHGAFTKLSPDDRDVEQIIAAETAADDYVEDKQQ
                     RTSDKWNEEGPWLLLLVLPMAALLFRRGLLFSIGFAVLLGVTARPSSALAFGWDDLWL
                     RPDQQAAKLFQQGETKQAAELFESSEWKGAAAYRSGDYEKAIEHFSQQNHSRANFNSG
                     NALAFAGRLQESLEAFERVLADSPQDVDAQYNHDLIEKLLKEQQKKKQQQEGQQGANQ
                     KNKQDQQDGSSQTQEKQNAQSAQDSSGDNAGTSDQQTAEGVKNNAEKESTAEKNNEKF
                     DEEKTESPNNEEGQKKIDGSAEKENAKNKKQDSTPVDLAKQQFLEQWLRKIPDDPGRL
                     LRNKMEREFQRRGKQNFTNEQYW"
     gene            31195..32901
                     /locus_tag="EYO68_01495"
     CDS             31195..32901
                     /locus_tag="EYO68_01495"
                     /inference="COORDINATES: protein motif:HMM:PF13584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein BatD"
                     /protein_id="HIC06508.1"
                     /translation="MMIKLFILGVLILAGNPAWGDVTAWVNKNPVVVGDMFQLHVEAK
                     NVDDAEEPDLSGITGLQILNRSVQNRTSIVGTSITRTLSWTYIMIAPSSGDYLIPALQ
                     VGNEQSTPIALKVTAYSQNQQQKYVSLDVEVVPKKVYPQQQILVRLRISRTGVQLENE
                     TITPFELKEALVEELSQKSYQTVKSGKKHKITEILYAVIPEKSGTLVIPQVRYQGEII
                     QRGPSQNNFGNFGNLFQKRGRRIFSTSTTQSVEVKPLPSGFKNWWLPTDKLVIEEKWQ
                     PDPPVFRVGEPVTRTVAIFANGVSGNQIPELKFDYPVTLKGYADQPLIETEKTEKGLK
                     GMRLEKWALIPSHPGKIELPGVSVSWWDIKSDTLQTAVISPKIFDILPVPENLQEIPE
                     KETPELQSNQAATTKVLQEKETAIESFPWKILAFGLAFLWCATMLMWFINKNNKAAAV
                     TKNKNKFIQDRKNALREATRNAEKAFSSGDPGIVQTALLKWGTAVWTDDPPQGLEQIG
                     ERMPELKNGINDLNSVLYGNNQTKESSLENLFNDFLKVSLLDKKFNNNKGQSQLEPLY
                     PE"
     gene            32904..34169
                     /locus_tag="EYO68_01500"
     CDS             32904..34169
                     /locus_tag="EYO68_01500"
                     /inference="COORDINATES: protein motif:HMM:PF00924.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HIC06509.1"
                     /translation="MIEYLSAFLNAYLTENLLELGLTTGMVESLVRGGLILLVLLLSW
                     VAHRVSQGPINRSIEKFAHYTIQQWDDILVEKHIVKRILYFIPLILLYVLSSPILTGT
                     SLLPLSQTLISVLFLIAGMMFLDAILSALLAIYGKSAIAKEISIIPFVQVLKLGLYFV
                     TGILLLSLLLQKTPLYFLSGLGALTAVLMFVFKDVLMGFVAGIQLIANKMVAPKDWIE
                     MPKYGADGDVIEITLTTVKVQNFDNTITTIPTYALINESFKNWRNMNLSGGRRIKRYV
                     NIDLGSIKFCSSEMLEQFKRIQLISQYIQNRQEEILVYNKKHQVDESTLVNGRRLTNI
                     GVFRSYVEAYLRQHPMIHKDMTFLIRQLSPSENGLPIEIYVFCKDTNWTAYEAIQADI
                     FDHILAVVPEFDLRVFQEPSGSDFQKIAL"
BASE COUNT        10218 a         6464 c         7626 g         9864 t
ORIGIN      
        1 aatctaggag tacttaatga agtttgcaag cattgtaaat aatttatgca aatagtggag
       61 tctgttttgt ttaagctgcc aaacttaata atttgttttg caaaggtttg tcaaataatt
      121 aagacaaaga cttataaaac attgattcac cttgaaacag taacgatgtt caggttaaat
      181 cgtagtgata gaattggtta aagagctaat ttttcttgat ccaatataat gaatgtttac
      241 tttaagtaac gtaatcgttt cagaattgtt ttgtttttta gggactaaat ctgagtggat
      301 ctagtcatac acattttaaa aatctcaatc aataaaggca ttgttgaata attcacaaaa
      361 atcgaatcgt caggttgatg ttgccatcat aggtgccggt tctgccgggc ttccggcttt
      421 tcgtgctgca cgaaaacact ctgaaaaagt tgtactgatc gaaggaggag tttacggaac
      481 tacctgcgcc cgtgttggtt gtatgcccag caaactccta attgctgctg cagaagcggc
      541 tcattcagta gagactgctt ccggctttgg aattcagaca gcaaaacctg ttgtcaatgg
      601 tagagaagtg atggagcgca ttcgaggtga acgtgaccga tttgtggggt ttgtattgga
      661 cgatattgaa aatattgatc aaaaacacca ccttcgaaac catgcaaaat ttcttgatga
      721 tcatagactt gaggttggtg aggagataat tgaagccaaa tcagtcgtaa ttgcaaccgg
      781 ttcttctcca aaactcatgc cgatgtttaa agggcttggt gatagactga ttgtcaatga
      841 cgatgtattt gaatgggaaa cactaccgga gtcagttgct gtttttggcc cgggcctgat
      901 tgggattgag cttggtcagg cattgcaccg acttggagtc cgtataaggg tttttgggcg
      961 ttcaggttca cttacacctc tcagcgatat ggcaatcagg gattatgcag aaaaggtgtt
     1021 tcaagccgaa ttccctctcg atacgaaagc caatctgcat ggtttggagc gtgaaggtga
     1081 tcaagtaaag attcgttttg ttgacaggga agacggcaag gaaaaggaag aaaattttga
     1141 gtatgtactg gcggctaccg gacgttcccc aaatgtaata aacttaggat tggaaaacac
     1201 cagtcttgaa cttgattccc gagaggttcc ggtgtttgac ccaaatacta tgcaatgtgg
     1261 agattcgtct atttttattg ccggagatgt aaataacact atgctgttgc tccacgaagc
     1321 ggcagatgag ggaagaattg ccggtgacaa tgccggacgt tttccggaca ttaaatcagg
     1381 tatgcggaga tctcctattt cggttgtttt ctgtgatcct caaattgcaa tggtaggaca
     1441 gtcatggggt gaactaaaag gacaggaaaa tgtagttgtg ggcaaactgt cctttgaggg
     1501 acaaggccgt agtcgggtaa tgcttaaaaa caagggtttg atgcatattt atgcagatcg
     1561 aacttctgga ctttttctgg ggagtgaatt cattggtcca caggcggaac atttggcgca
     1621 tttgctgtca tgggcacacc agcagaaaat gacaattcct caaatgctgg aaatgccatt
     1681 ctaccatcct gtgattgaag aaggtttgcg cacagcactg cgtgatacta atgtgcagtt
     1741 aagtttgtga cattctggtc acagcaaagg ggccgtattc ttgttcaaat ctccaactgg
     1801 atggcataaa tgtccgaaca caactatgat gtaatcataa ttggaggcgg tcctgccaga
     1861 ttgttcgcgg ggatcgcctg tgtcaggaat gaactacgga cattggtttt ggaaaaacaa
     1921 acatatccaa tcgataaagc ctgtggtgaa ggtattatgc cctcgggtgt taataatttc
     1981 aagaagttgg gagtagctcc tttcctggaa aaatatccct accacctttt taaagggatc
     2041 cgctatgttt ccgcaaaggg tagtgttgca gaagcaaatt ttgcagaagg ttgcggatgg
     2101 ggtgttcagc gaactaagct atctgccgca ttgcttgaat gtgccgaaac tttaccaaat
     2161 cttgaaatac aagaaggggc ttctgtagca tgttctttag aagaaaatcc tgaagtcagt
     2221 attaaaaata atgtttttca cccaaaacta ttgattggcg cagatgggtt gcattcacgg
     2281 gttagaaaac tggcaaatct ggaagcaaca atcccaaagc tgaaacgctg gggtgttcga
     2341 caacatttta atgttgaacc ctggagcgcg tttgttgaaa ttcattgggg ttcagggctt
     2401 gaagcctatg tgactccggt ttctgaaacc agcataaacg tcagtttatt gtgggacgca
     2461 aagcgctaca aacctgagca tggcggcaaa aagttgtttt gctcaatgct aaatgaattt
     2521 ccggaactgc caggatcgct taaaaaacgc agatgttctg gatgagccgg tggcaaaagg
     2581 tccgttgttc cagaaaacac tgggtgtggc gaatggaaaa atcttgctga ttggtgatgc
     2641 cgccggcttt tttgatccta ttactggcga aggtatcgat tttgcggcca ggcaggcact
     2701 tttgttagaa aaatatgaag agccggtatt gaaagagaat tctggaaatc ttgtaaaggc
     2761 aatgtttgat tattcacgag caagtgctca aatctatcga cgatatcaga ttatgaccag
     2821 tttggtactg ttacttcgct tatggccaaa gttgacggat ggtgtgattc aggttttgca
     2881 ctcctttcca gcattgtttc aaaaactgct gtctgtcaat atgcgctaac actttgaagg
     2941 aactggttag tgaagtgtaa cccggcatct gcgaacgatt tcaaaatatt gcaggatttt
     3001 aagctgccat acggaatgtt aatttggaaa caaaaaaacg agttgtagta acaggccgag
     3061 gtgttatttc gccaatcggg agttctgttt cagactattg ggaagggctt tgtaatggtg
     3121 tctccggagt ccgtaaaatt actgaatttg attcttcagg ggcatcctgc caaattgcag
     3181 gtatggttac agattttgat tctgacgtac attttagtca tcgtgaaaaa aaaagatatg
     3241 agcgcttcac tcagtttggt ctggttgcgg cactaagggc tgctgaagaa gccaatctct
     3301 tggatcagga cggtgattcc ggagaaattg gtgtgatgat ggggaccggt gtagggggaa
     3361 taacctctta tgcacaagcc tacgatgcac tttacgctgg accggaagaa cggccaattg
     3421 acgcttacac cattccacgc ataatgaaca gtgcggtttc atcggcaata gcaatacgtt
     3481 tgggaataaa ggggccgaac tttacactga atacagcctg ttcttcagga gggaatgctg
     3541 taggccaggc aatgcagtgg attcgagaag gccggttacg cgctgttatt tgcggaggtg
     3601 cagaagcacc tgtcacgtac ggtcttctac gtgcatggga cgcactaggt gtgctttcac
     3661 gtaagtataa cgaaactcct gaaactgcct gtcgaccgtt cgacaaaaat cgtagtggtt
     3721 tcgtattagc cgaaggagct gctgttttag tactcgaatc attagaatct gcaaataaaa
     3781 gaaatgctcc agtctttgga gaaattgccg gttttggttc aaactgtgat gcttctagtt
     3841 tgacaaatcc ggatgaaaac ggagtggcag aatcaatgaa attagctttg gacgatgctg
     3901 aaatgtcacc agattccatc ggctacatca atgcccacgg aacaggaacc cgtcttaatg
     3961 acccgctcga atgccgtgca attcaccggg tttttggcga taggtcagta aagttgccgg
     4021 tcagttccag caaatcattg tttggtcatg caatgggagc atcttcagcg ctggaggccc
     4081 tggcgacttt gctggcagtg cagaacgact gtgctccgcc aactcttaat tgcgacgacc
     4141 tggaccccga atgtgtgatt gatcccgttc catataattc ccggaaagtc cagattgaag
     4201 cggcactttc caattccttc gcctttggag gtagtaatgc agtacttgtt tttcgaaaat
     4261 ggaaaccata acacgcaaaa aaattgagca atcctgtctt caaacaattg ccgtggaaat
     4321 gggcttgaaa tcaaatgatc tcaatacaga aatgaatttg cgtgatgatt tggatatcgt
     4381 ctcccttgat gcaatgaata ttatcatggc actcgaagat gagttcaaaa tagaggctga
     4441 tatagaaact gtcctggaaa tgcagaaagt ttctgatatt attgatcatt tagaaatacg
     4501 tataaattca taaagaacgc ggctacttga aaaatataaa aaagtttgat ctctcatttc
     4561 gatcaataaa ttatagtatc atagactgtc cttcccgatg cctcatatta tcaacacagc
     4621 cacagctttc ccgacgcatt atcattcgca gcaagaaata agttttgcgt tgagagcagt
     4681 gtggattaaa aaaggtttgg atgtagcgat ctttgaccgt ctgcagaaag cagtaactgt
     4741 tgaaggccgc tatttggctt tgcccatgtc tgaatattac aagctggacg ggtttgcaga
     4801 ctgcaaccgt gcatggctgg aagtggcttt agagttgggc gagcgggtta ttaatgattt
     4861 actggaaaaa actaacttgc atccgcaaga aattcagatg cttatgagtg taaccagtac
     4921 aggagtgact gtgccttcat tggaagcacg cttgatgaac agaattgcct ttgctcagga
     4981 tttaaagagg gttccattgt ttggtttggg ctgtcttgcc ggtactgcag gggttgcacg
     5041 cgttggcgat tatttgaaag gacatccgga ggaagcggct atattgctta ctattgagct
     5101 ttgttcatta actttacagg ccgaagacct ctcgattgca aacatcattt cgagtggact
     5161 ttttggagac ggagcggcag cagttttgct ggttggcgac caacatccgt tggtgaaaag
     5221 taatgaaaaa cgaaatagca aagctaatga gtttcatggt tccaaagttt ccaaaggttc
     5281 aaaagatgaa agtgaaagag atcatgagtt tcataagccc agggttttga gcagtaaatc
     5341 aattttcttt cctgactcag aagaagtcat gggttgggat gtaacagatc gtggattgaa
     5401 aattatgctg gggcaagggg ttccaggcta tgcgaagcag ttacggccgc atgtggacag
     5461 tttccttgca gaacacggac tatccattcc tgatattgct ctttggctca ctcatccagg
     5521 aggaccaaag gtgattgata caattgaatc atgcttggag ttgcctccaa aatcacttga
     5581 taaaacacgg aaacacctcc gtgaagtagg taatatttct tcaacttcag ttctggttat
     5641 acttgacgaa tgtttaaatg gtccggttcc gaaaatctat ggtaaatccg ataccggaaa
     5701 ttttggaatg atgatttcat tgggaccggc attttccggt gaattggttt tgctgcaatg
     5761 gtgagccttt ggggattcat gttgttggta gcattgctgg ctgcacagcg cttgtgggaa
     5821 ttgagacgaa gccggcgcaa cgaaatcagg atgagggaaa aaggcggatt cgagcgtttc
     5881 cctgaacatt atcgagtttt ggtcgcctta catgtttcgt ggtttgcagc aatgctggca
     5941 gaggtctggt actttaggac agcaccgcct ttatggttgg tgatagcggc attttcagga
     6001 ttagctgctg gacaaaccct gcgctatttt gccatccgga cactcgcaga gcgttggtcc
     6061 acccgtattt acgttgttcc ggatgcaccg ttgattaagc acggaatata tcgatttgta
     6121 cggcacccga attatttggg agtgatattg gaaatagcca gtgtaccttt gcttcatgca
     6181 gcatatggaa cggccgttgt tttttctctg gccaatctta ttattctccg ccaccgtata
     6241 cgtttggaag aactcgctct agatgaaatt tagctgtgta aaaatcagtt ttaactgatt
     6301 tattgaacta gataatccta ttttttcagt acctgttgaa atttaaactg gacacaaaat
     6361 tgttgaaatg actctcagcc tgcacttcga taaaggaacc atccagcttc acggcatggc
     6421 gggtagatat atgcagcatc ttgacggaat ttcctgggat gagcggacaa acagttaccg
     6481 tactcctgca gccaactacc gtaaacttgt cacagcactg tgtgagaaga acattagctt
     6541 ccaggaccat gcccgaaaat tttctgcaga aacttttgtc ctgaaaaaga acattagacc
     6601 tcgttctttt caaagtgaag ctgctgaagc atggttgtca gaaaagcacg gagtagtaac
     6661 tttaccaacc ggtgcaggta aaactattct ggcagtaatg ctgattgcgg aaaccggacg
     6721 accaactttg attcatgttc caacgattga tctgatgcgg caatggaaag aggttttaag
     6781 cgaatatttt gaaactccaa ttggtttact tggtggtgga ataaatgata ttcagacaat
     6841 tactgtagca acttacgact ctgctttgat ccatgtaaca catcagggta atttgtttgg
     6901 actcctggtt tttgatgaat gtcaccattt gcctggtgag caaaatcaat ttactgcact
     6961 tggttcaatt gctccattcc gcctgggttt gactgcaact ccggaacgtg ctgacggaaa
     7021 agagcaagtc ctttacgaac tttgcgggcc tctttgttac gaagttcata ttgacgagtt
     7081 aagcggtcgt accctggcac catacgtggt ggaaacaatc gaggtagata tgcgtccgga
     7141 ggagcgtgag caatacgaga tttcacgtga atgctatact gattttttac gtaaagcacg
     7201 tatcagcttt cagcatccaa gcggttgggc tattttttta cgcagaacca gtgaaagtcc
     7261 tgaaggccgg aaagcattca aggcttattt gctgcaaaag aagctcagtc aggcatccgg
     7321 agcaaaagta gatcgtttgt gggaactgat tcagcagcat aaaggtgacc gtatgctggt
     7381 ttttacgcaa gacaacgaaa tggcttaccg cattgggaat aatttcctgt taccggtgct
     7441 tacacatcat acaaaactac cggaacggga ggcttttcta aaatcattca gatcaggcga
     7501 atatccgatt ctagtcactt caaaagttct gaatgaagga gttgatgttc cggatgcgaa
     7561 tgtcggcatg attgtttccg gaagcggcag tattcgggag catgttcagc gtctgggacg
     7621 aattctgcga gcacgtcccg gaaaacaggc catactttat gaacttgttt ccaaggatac
     7681 cggagaatat tttaccaacc agcgacgtag aaaacaccgt gcttacgaag gacctgttgt
     7741 gcttccggac gaatcggggc aaaattctgc ccaaattaat taatccggaa gcaacaaaac
     7801 ttttagaaat tgcagaagaa ttactcgcag ttttttccgg aagcgtaggt caagtccgtg
     7861 aaaaacttga ggaagagaca aagcaggttt tggacggttt tccgggaaac gcaattgtgg
     7921 gccgaggttt ggaaaaactc ctgcttgacc gtaccgaatt tgacacggaa gcaaaaacgg
     7981 aattaactga tctcagggaa aaagtatttt tcaactctgc tgcatttttc agtgctaaaa
     8041 aagcgtttcg ttttccgggt gttgagatgg gggataaagc tgatttaggc cattatcaaa
     8101 ttgaaatagc caaatcagta ggaatatcta ttgagcagtt gggacggcaa ctgtatggag
     8161 atttgccgcc ttttcagcag gtgctgcgtt tccgcaaaat gactgctgca ggcttactgc
     8221 atcgttacaa ttgcgctcag ctccaaggct tgcttctgcg ctgcgaaggc ctaaatgtta
     8281 gcctgcctga aagcaaagca gccgggttgc ggcaactgct taaatatttg cgtttcaata
     8341 aattacttgc aaagatcagc cacgatcaaa gaaagaaaaa aacattgatg ctggaaattg
     8401 atggtccgct gagtttgttt gtaaacacac aaaaatatgg tttaaacctc gccaattttt
     8461 tccctgcaat tttgcagcag cctgaatggg aactggatgc agaagttaga atccgtaaaa
     8521 accatgttca cagcctgcac cttgatcaat cttgtggaat tcgctcccac taccgtcagt
     8581 ttttagccta tattcctgat gaaatacagt tgttcagtca gcaacttgct aaacaaatac
     8641 ctgactggga actgacatct tcagcagatt ttattccgtt gaccggtgaa tcgctttgtt
     8701 tcccggattt tctgttgaag caccattctg ggaaaaaagt gtcaatggag ctttttcata
     8761 aatggcatgt ggctcccctc cagagtagac tggaacaact tgattcacaa aaaggggcac
     8821 ctttgctgat aggcatcaat cgcagtttac tcaataatat tcaactggcc gagcaagtag
     8881 aggcatccac gtatttttca cgctatggat tcttttttag agaagcccct acaatcacaa
     8941 aacttcgtcc gctgcttgac tcctggttga gtaatgttca aaagacaatt tagagcataa
     9001 cttttcgttt ttcacctcac ctttgcaata tctttatttt ttcagcctct atgctctatc
     9061 tcagggtaaa gagctgaaaa gaaaacaaag tcattttaaa aaagtcccaa gttacctaag
     9121 acttagtgcg gtcactaaga gtatgtctcc tgtggtaaag aacaaaaaga ttactgcact
     9181 caccaggaag gctaaacgat gttaggttca ggtaccaaac aaactttcct tcataattct
     9241 atgttgcggt aacaggctct tgaagaaaat taacaaaaat gtcagaccga tgtctatttt
     9301 catgaggata ttgctgtaaa gaaaataacg gtcagaatag atacctttaa tcaaaatacg
     9361 ggaaaaacac aaaaggcttg aaattgtcac ttcaaatgta attaaataaa cgttgagttt
     9421 tgtttcattc agttttgtgg gcaagactgt cttcatttcc ttacaaaatt aatttttatg
     9481 aattccactg tagaagaact tggcaaactg aagtattccc ttgcccttga agtctccctt
     9541 gatgaaataa aaccaaccta caatgcaatt tatcgggaac tgaaaaaaac cagactcaat
     9601 ggctttcgcc ctgggaaaca cccaaaaggc tggttggaca aaaggtttaa ggttgctatg
     9661 cagcaggaag ccgtcgacag ggttattcct ggatatatgg atgatgcact acaagaacat
     9721 tccttgcggc cggcaaccat gccgataatc cagaaaatcg actttgacct taaatctcca
     9781 ctttctgcta ttctccattt tgaaatagca ccggagctac ccaaactgaa ttatgaaaaa
     9841 atcgtgcttg aacgcaagga tattgaggca gtatctgcaa aaaatatcag tgaagaatta
     9901 gaaggtttaa ttcagcgaga agaggtactt gttcccaaag tcggagagga tgtaaaagta
     9961 gaaaaagacg attgggtttt aattaattat gaaggaacaa ttgaggatga aaaatttact
    10021 gactcaacag caaccgaaat gcagttcaaa atcggttctt cagatttgga agagtttcac
    10081 tcgggcctga tgaacatgtc atctggagaa gaaaaagatg ttgagcttgc gctgccggaa
    10141 cggtttggag aaaacgcagg taaaaaagct attttcaaaa tatatttaac agaaatttct
    10201 gcagttaaaa gaccggaaat ggatgaagac ttttttaaga aatttggggt tgcggatgaa
    10261 gacgaattga aagaaaaagt aagtgaaaat attaaatctc gaaaaacagc agagcttcaa
    10321 agtgaatacc gaattgctgt tcgtgcacaa ctttctgatt tatatgacga ttttaatttg
    10381 ccggaagaac tcgtaaaata tgggcaggaa caagttgaaa gagaattgga acaggcatca
    10441 tctgaaaaag aaattccgga agaagaaaaa gagaaaagac gtcaggaagg aatagaaaat
    10501 gcgaaaatgg atttacgcat gaaattcata ctggactcga ttggtgagca tgaagaaatg
    10561 aaatttgaca aaaatgaggc agctcgtgaa tttgtcggat tggcacaaat cacaggacaa
    10621 tctccggacg aacttattaa atcgcctttc ggacatgaca tgtacgagcg aattgttgtc
    10681 cgcaaaaaag gagatgcaac tcttgacaga gtggtggctc gggtgtttgg tgacccgatt
    10741 gaagagtttg ctgctgaaga tcatgagcat gtacacgatg aaaattgtga gcatgatcat
    10801 tcctgacaaa cttgcattca agtacaaaaa atctttcttg tttgttaacc acacgaaaaa
    10861 ggaatctatt agttcagatt tttcaagttc cttgattctc gctttagtag tgggatagag
    10921 aatgtttaaa gcagcacaac tcgtttataa aaaaataact tcttaatgat taaccttttt
    10981 tcaagtatgc tatgtcgcta ataccgatgg ttgttgagca ggatagtcgc ggcgaacgag
    11041 cctacgacat ctactcaaga ctgctcaaag accgtattat ttttctcggc tcgccagttg
    11101 atgataatgt tgcaaatgta atgatagccc agcttttatt tttagaagca gaagatccgg
    11161 aaagtgacat ttcactttat ctgaactgcc ctggtggttt agtaacttca ggaatggcaa
    11221 tttatgatac aattcagtat ttaaaaccgg atgtgcagac tatttgcatt ggtcaggcaa
    11281 ccagtatggg ggctgtttta ttagcctcag gggcatccgg gaagagacag gctttgccgc
    11341 acgccaggat aatgattcat caaatgatgg gaggattttc cggacaggca gaagatattg
    11401 atattcaggc aaaggaaatt atcagaatga cagatgtttt gaacgatgtt ttggctcgtc
    11461 atactggaca taagattaaa aaaattgcac aagataccca acgtgattat tatttttcca
    11521 gtcaggaagc caaagaatat ggtattatag acgaagttat gcaaaacaga aaactgaaag
    11581 ctttataaaa gaaagataac tatgaattct gttaaagagg ctctcttgcg ttgctccttc
    11641 tgtggaaaaa cacaggaaga agtaaagaaa ataattgcag gtcccacagt ctacatttgc
    11701 gacgaatgta tcgacctctg caatgagatt atggaagagg aatatcagaa tgagcatgct
    11761 gatgagtttc aaaaagaatt gctaaaacct gcagaaatta aagatgttct tgataattat
    11821 gtggtgggac aggatcggcc caaaaaggta cttgcagttg cagttcacaa tcattacaag
    11881 cgcatcaaag ctaatttcga taaatcagaa gtggaactgc agaaaagcaa tattctgttg
    11941 attggcccta caggaacagg taaaactctg ttggcgcaaa cactggcacg gattctaaat
    12001 gtcccttttg cagttgcaga tgctacctcg ttgactgagg caggttatgt tggtgaagat
    12061 gttgaaaata ttgtattaaa attattgcag gctgccgatt atgacataga acgcgctgag
    12121 caggggataa tttatattga tgaaatagac aaaattacca gaaaaagtga aaatgtttca
    12181 ataacgaggg atgtttccgg agaaggtgta cagcaggcac ttttgaaaat catcgaaggt
    12241 actgttgcaa acatacctcc acagggtggt cgtaaacacc ctcatcagga gacaatgcag
    12301 gttaatacag aaaatgtact cttcatttgc ggaggtgctt ttactgccct tgatgacata
    12361 attagaagac gcataggtcg aaatgccatt ggttttgggg cagatgtaaa taagaaaact
    12421 cggaatgtag aggtcctgtc acagctggaa ccagaagatt tactcaaata tggtttaata
    12481 cccgaatttg ttggacgcct gccggtagtc gcgactttgc atgatttgga agttgaccac
    12541 ctggtgcaga tcttaaaaga accaaaaaat gctttaattc gtcagtatga gaaaatgttt
    12601 gagcttgata atgtaaaact gacatttaat aaagaagcaa tacacactct tgcaaaagaa
    12661 gcgatcaagc ggaaaaccgg agcgcgagga ttgcgctcta taattgaaaa ttcaatgctg
    12721 gatttaatgt atgaaatacc ctcagatcct gacattaaag aagttgagat caataaggat
    12781 tccatcctgc aaaaggccga gccaaagctg accagaaatc cagcaagcag tattcctcga
    12841 gcgaaagcaa gctgatttta tataataaat ctttttgttt cccaccacat tattaaataa
    12901 ataatatgga aaatttagat gtatatggtg tttgcaatcc attaattgat ttattgagcc
    12961 atgttccaga ctcgttttta acagaacgtg gtttgaataa aaacagtatg aatcttatta
    13021 cccttgaaga gcagcaaact ttcttactgg ctttggcggc aggaaaaata tcggtagaat
    13081 ttgctgctgg aggttctggt gcaaatacaa tgatcggcat agctcaactg ggaggacgaa
    13141 cagcttttag cggtaaaatt ggcagggatg agcatggaaa gatttaccgg gaaaaactgg
    13201 aagacctcgg agtcaatgct gtacttggag aaggagacgg taccacagga agcagcttga
    13261 ttctggtaag tgaagatgga tcacgcgcga tgaatacata tctgggaatg agtcaggaac
    13321 tgcaggtgcc agatattgac ccggaagtta ttcaagcttc aaaatatctg tatcttacag
    13381 gatatttatg ggacacagag gtgcagaaaa aaacagtaat aaatgctttg gatgaagcaa
    13441 aaaaacgtga gataaaagtt gctctgagcc tttcagatcc tttttgtgtg aaccgccata
    13501 aggatgattt taaaaaactg ctgcaaggtt acgtaagtat ggtattttgc aatcaggaag
    13561 aagcattttc acttttggat acaactgtaa cacaagaagc tgtggagatt ttatcacaat
    13621 ggactgaaac ggttgtattg acattaggct caaacggtgc cttgatttca catcttggtc
    13681 aaacttttta catcgacccc tctcagattt gtgtggaaga cacaaccggc gccggagatg
    13741 cctttgcggc aggtttcctt tacggaatga ctcatgagtt ttctcctctt gaaagcggcc
    13801 gtattggagc tgttttggcg ggggcagtga ttgaacaaac cggcccacgc taccagggac
    13861 atgcccagac gctgatacgt gaaaatattg gtataaagct ttgaatttat tataaatact
    13921 gtcttcagaa acttgtttac tttctatgga acttcttgcg ccagccggta atttagacaa
    13981 attaaaaact gctgttctgt atggtgcaga tgcagtttat ttagcaggtc caaaatttag
    14041 tttacgtgga gcttcagata atttctcgga tacagagttg tgtgaaggga taaaatttgc
    14101 gcatctaaat aattgtaaaa catacgtaac attaaatgcg tttttacatg ataaggaact
    14161 cagagagctt ccggaatatg tacgcttttt agagaaaaca agtgtggatg cagtgattgt
    14221 atccgatctt ggtgtgctga cggttgttca gcagcactca aaattgccgg tgcatctttc
    14281 cacgcaggca tcatgcttaa atattcattc tgcaaaagtc tggaaaaatc tgggagttaa
    14341 aaggcttgta ttgggacgtg aagtatctct ggaagaagcc ggatgcatcc ggaaggaagt
    14401 agatattgag attgagctat tcattcacgg tgcaatgtgt atggcttatt ctggtaactg
    14461 cacaatctca aattacacag ccgggcgtga cagtaatcgt ggcggatgta tccaaagctg
    14521 tcgtttttca tattcagcaa ttccggtcaa caatgcagta gcagttaatt ctacggataa
    14581 tactccgagc tcattgatga gttcaaaaga tttgcggggc attgacttgc tgccaaagtt
    14641 cctcaaaata ggtgtagaca gcattaaagt agaaggtcgc atgaaaagcc ctctgtatgc
    14701 agcaacaacc acaagtgcat atgctctcgc attaaaatgg tgtaattcaa ccgtacaaga
    14761 gcaatggtcc gagaaacttc aagagttgtc cggcatgttg gaaaaaattc cacacagagg
    14821 ctacacagat ggttccctaa ataatgaagc agatgctgaa agtgtttatc aaggtgagcg
    14881 gaacgggagg aattccggct atgagattgc aggaactgtg atggaagttg aagacggaaa
    14941 gtctttcacc atgctgactc ataattcttt tgaccgaaat aagaccttgg aaattttgac
    15001 atttgacgga ggagtgatca agatttcaac ccaggaaatg aaggacttga acaacaagcc
    15061 ggtaaaaaga gcaaatccca gcagactttt tcgttttgcg tgttcagaag cagcagaaca
    15121 atcagctgat gtttctggaa tttcccaagt ccagccactg aacgtagtcc gtttgaaaat
    15181 ttgataaata acaatatttt tattttattc tgcctctgga tattctggtt atagaaacac
    15241 tcctcgaaaa aagaagaaga agaaaattga caagagggcc ccgactggca gtgtaataac
    15301 ccatgacatg aaaattccac ctacaaccct caaatccagg gcaccaattc cgcgtgccag
    15361 tccgactccc attacggcac caactgcaat ttgagtagtt gaaactggaa ttccagtccg
    15421 ggaggcaatt accactgtag ctgcagctgc aagttctgcg gaaaagccac gtgtaggcac
    15481 gagctcagtg attttactgc cgattgtttg catcacccga taacccaaag tcgataatcc
    15541 aagaactatt cctacagcgc ctgtcaataa aatccaaagt ggcagatttg atttctgggc
    15601 catttcgcca ccgctttcca caatactgac aattgcagcc attggaccaa taccattggc
    15661 aacgtcattt gatccatgtg caaaagccat tgcgcatgcg gtgaagatca tcattatgcc
    15721 aaaaatcttt tcaatttgag taaactgatc atgaatgctc tctgcttctt tcattttaag
    15781 tcgattgatc ataaaacgga caattactgc tactataact ccgaaaatta ctgcagcaaa
    15841 aaaactttgt ccgacactaa aagtaatatc taaatgcttt aatcctttga aaagagttac
    15901 cagcgaaagc aaaaatccaa caagaaagac ataaaacggt gcatatcgtt ttgcgttcag
    15961 aaaaggagtg tctgaatcaa gaataaattt ctgaatactt cgcatcagga ggtatgcgag
    16021 tgttccgccg acaagcggtg aaacaatcca actgaggact attgttccta tttttgacca
    16081 gttaacagca tcgactccaa tgccgaccac tgcaaatcct actattgcac caattatact
    16141 atgcgtggta gaaacgggcc agcccatcca actggcgaat gttaaccata gtgcggctgc
    16201 aagcagggcc gataacattc cccagaccag aatctcaggc tgatccatga ttggagtagg
    16261 atctataatg cttttccgga ttgtttttgt aacatgacct ccggcaagga atgcaccaga
    16321 aaattcaaaa attgctgcaa taataatggc ctgtttgacg ctgattgctc ccgatcctac
    16381 agatgttccc attgcgtttg caacatcatt tgccccaatg ccccaggaca tgtagaaacc
    16441 cagaaccaca gccaggatca taaaaactga accgtattca acaagcatta tttcccatta
    16501 ttattgaatg aaaagtcaga attaattgtt agttggaagc aatcatcaaa cgtaaacggt
    16561 ttcctgtttt tttggaataa tcagcaatgt caccaatcaa ttcaaaaatt ttataccaaa
    16621 agaccacgtc tacagccttc atactgtctt ctatatcaaa tattctgcgt accagtgcat
    16681 cttctaaact gtctgcttct gtttccgcat ttgaaacctc atcaatcatt tttaataatt
    16741 tgtcgatctc atgtctccca aatcctgatt caagcacatc tccaaattct tgagagagac
    16801 cctgtgccat gtaacagact tgctgacttt tttctacaaa ctgaataagt tcctgacaca
    16861 aatcagtcgg caggcacatt cttctgaggg taagtaaatt gacgatgtcc tgtgttacat
    16921 ctgcaatggt gtcctgcatg tcaagcattt ccaataaatc ccgacgatta atgggcatga
    16981 aaatactacg gggtagatgg ttgcggacct cgtttttgat tatatctgct tcttcttcag
    17041 caagtgatat ttgctgctga atttcttcga cttttgaata gtttccctga tgcagtgcat
    17101 caaaaaaatc tttaagcggg gcaacacttt caaccacttt gtccatgtga tccctcagtg
    17161 gtttgaaggg cgactttgca aacatagaac tgagattaat catgaaagat ccttttgcta
    17221 gtttccggat atagatttag acagacaata tttttaattt catgagttca cagagttatt
    17281 ataaaaagta tcaatcttca tttactccat tagtcttaaa ccccccactt tgcaggtgct
    17341 gatctttgcg agcctcttcg agatctgcca caatcatttc ttctatcatt tgctgtaaat
    17401 caagtgtcgt tttccaaccc agtttttttc tggctttagt agcatcacca atcagtaaat
    17461 caacttcagt cggtctgaaa taagtgggat cgacagaaac aacagtattt cctgttttag
    17521 tatcacgacc tacttcatca acacctcttc cggaccatcc cagggtgatt ccaacttctg
    17581 caaaagctct ctcagcaaat tcacggattg tggttgttac tccggtagcc agcacaaagt
    17641 cctccggctt ttcctgttgc aacatacgcc acataccatc tacaaaatcg cctgcatgac
    17701 cccagtcccg ttttgcatcc aagttcccca gatatacttg ctcttgagta cccattgaaa
    17761 ttcggacagc cgcacgggta atttttctgg taacgaaagt ttctcctcga acaggtgact
    17821 catgattgaa gagaataccg ttgcaggcat acatgttata ggcttcacga taattgactg
    17881 tgatccagta tccatataat ttggcaacag cataaggtga acgaggataa aaaggcgtat
    17941 tctcattttg aggaacttcc tgcgctatgc cgtacaactc gctggtagag gcctggtaaa
    18001 actttgtttt ttcaaccatt cccaaaagct tgattgcctc aaggattctt aaagtcccaa
    18061 gtccatctgc atttgcagtg tattccggtg tttcaaaaga aacatgaacg tgactttgtg
    18121 cagcaagatt atatatttca tctggttgta tttcctgaat tatccggatc aaattagtgg
    18181 aatcagtcag gtcaccgtga tgcaaaataa aattacgttt acttacatgg gggtcctgat
    18241 acaaatggtc tattctgcct gtattaaaac tggaggagcg tcttttaagc ccatgaacta
    18301 tgtatccctt tttgagaaga agctcggcaa gataagctcc gtcctgaccg gtgatgcctg
    18361 tgataaatgc cacttttccc attttaagtt ttttgattat aaaaagttat atgtgaaatt
    18421 aagtttttgt ggaggatgac aactcgtccg catgtaaatt tttcagggct ttgccgaccc
    18481 gatccaaggc agtttgcaga tctgcagttg aaatattcaa cggcggcaaa aaacgcaata
    18541 catccggtcc agcaaccaaa accaaaacgc cctcttcctg gcaggctgtg accattgtgg
    18601 aggctttccc tttccaggcc tttgataaca cggcaccaag catcattcca cgaccacgaa
    18661 ttgtttcaaa caaactaagt tcatcgttga gtgcttccag gttatttttg ataactaagc
    18721 cttttttctg tacctgctcc atcatttgcg gagtattaac ttttcggaat gcagcactgg
    18781 caacagcagt gacaatcggg ttccctccaa atgttgttcc atggtctccg ggtttgaagg
    18841 tttgcgcaac cttttcagtg caaagcattg cgccaatggg cagtccaccc cccaaagcct
    18901 tggccatggt aacaatatcc ggtttaagat ttgtcactga attatccggc tgttcagaat
    18961 cttcagaatt ttcccactgg tagccgaata attttcctgt acgacccatc ccacactgaa
    19021 tttcatcaaa aatcagcagt acatcatgct ggtcacaaag ttttcttaaa tgggacaaaa
    19081 atcctggttt tacaggattg acaccgcctt caccttggat tggctcaatc agtactgcgc
    19141 aggtttgtgg cccaatcatt tcggtgactg tatcaaaatc attaaatgga cagtagcgga
    19201 atcctcctgg aagcggctca aatccctgtt gatattttgg ctgtgcagtt gcagtaactg
    19261 ttgctaaagt tcgtccgtgg aaggaacctt caaaagtcaa tatttctcgt ttccctgctg
    19321 gttgacttaa acttgaatat tttcgtgcaa ttttaattgc cgcctcatta gcttctgctc
    19381 ctgaattgca gaaaaaaacc cgatctgcaa atgtagctgt cactaattct tcagccagct
    19441 taattgctgg ttctgtaaga taaagattgg tcgtatgcca tattttccca gcttgtatat
    19501 taagtgcctg cagtaggtct gagtcaaggt gacctaagct gttgacagaa atacctgaac
    19561 ctaaatcaat atactcattt ccgtcactgt cccaaattag tgctccttta ctgtgggtta
    19621 aaatcaggct tggtgcatat gttggtaaaa ataccttttg tgcagttgcc agaagatcag
    19681 cggcttttgg gttgttattt agtttcaatt ccgactcttt ttcagtgctg gtcttcttta
    19741 attctgcttc tgcagtttct gttgcagaga atttttttga aacagtactt cctacattca
    19801 gcagacgctc acgatttgcc atacaaaatt caacaaaacc ctgttcgcca actgccagtc
    19861 taatcatgcg cggcaaagcc tgatacaagc gtctaaaagc agttcgcatc acaacttcgt
    19921 ttttcagggc atcagtcggt aaattcccca gcttttcttt gatcaacttt tggattcgtg
    19981 gtagttgttc ttccagcagc atagttgtgt ttgttcaatg atttttcttt ttactttaga
    20041 actttttcca aaggttttta gcttttttcc ccggggaata attaacaatt tatactaacc
    20101 tatgtctatt attatttcaa tcatagaaag gaagaaatct gttcaacaat tacaaactga
    20161 attaattcac aacttgcctg tttaactgaa tttttgataa tataattctg catttatgca
    20221 tttaccagtt gtattatgaa tgataccaga atccattttg gtatttgaga cacagccatc
    20281 ttattgaacc agttctttgc tttatgtttt tgctgtgcag gtctataaag tattaaaaat
    20341 gtttgcttaa atgactgagt aagccatctc ccccgaattt atcaggtggt tgttttcagc
    20401 acaaattgaa gtattcaaac tgaccagggc ccataattta ttcaaattca aagtacctac
    20461 attaaattta atttggacca aacgtgactc ctctgaaaat aattcaaggc tgggttgtgc
    20521 gctatcccaa acgaattctg tttttaactc tattcctggg agcgtcagtt gttcccagct
    20581 tgttgtttct taaaaatgat cccagtcccc atcttctgcc agtcagccat cctgcacgtc
    20641 aagcgctgca gcagcttcgt gaagatttta ctggtacaaa ttctggtgtt tttatcatgc
    20701 tggaagctaa ggacacgatc tttaaaacca atacgctgga acggatccaa cgactgaccg
    20761 aagcaataca aaacatgcag ttgctttcaa ctgaagactt ggaggctttg aatgtaatag
    20821 cagaacaaat gtccggaaaa gaaggtctac gtctgcaaaa gctgcttccg aaagaagtta
    20881 aagatctaaa tgacatgttc tggatggagt ttgaagaaat gagagaaact ctggaaaatg
    20941 aaggccgctg gtttccggaa tggaattccc tgatccggaa tattgaagtt cgttctgctc
    21001 cggtagtgga ggtgacatcg atttcgaaca ctgatgatat ttatggttca gaggaggggt
    21061 tgacgattga gaagatattt gaagtaattc caggaactcc gggagagatg aaaagtttgc
    21121 gccgcagagt tttagaaaat gatttgttca ggaattcctt cttcagcgaa gacgggcggc
    21181 gtacaggaat ttttgttgaa ttggcaatgg acgaagatga ttctgagatt ctgtattcaa
    21241 cttatattgc attggaacgt atttttgagg aaaaccctgg aattgataag cattatattg
    21301 cagggtttcc cattgttgcc gccactctgc gtacagtgat tgatcaggac actcagcgct
    21361 ttttcccttt tgttgctttg ctggcagtct ttttcctttg gctgacattc aggagaccaa
    21421 gcggggttgc tgttccaatg ctggtggttg gattttcgat attgtttacg ctggcaatta
    21481 tggttgtttt tgaggttcct ctcaatacca tcacctcggc tttgccggtt ttcctgattt
    21541 cgattggagt cgcagacgga attcacatgt tttctgaata ccgtgaaaat cgtatagagg
    21601 gacttccccg tgaaaaagct gtctgtttga tgctggataa acttgctcta ccggtgacaa
    21661 tgacttcaat cacaacggca gttggttttt tatcgctcac agtcagcgac atagtaccga
    21721 ttcttacgtt tggaattttt gttgccgttg gaacattact ggcaatggtc ctttctctga
    21781 tatttattcc cgcattgctg atggtacttc cggaaaaagt atcagcgtct caggaaggtt
    21841 ccggaacaga tgaaaacctt tccagcggag tacatcaggt gaatttcatg gatcgtcttt
    21901 ttcaaaagtc tcttgacgtt atgacagcat gggttctacg aaacgcacga ccagttttgt
    21961 tggcagcact agcgatttgc gtaatttcaa tttatggact ttcacaagtg aaagtggaaa
    22021 gcagtttgga aagttatttt caggcagatg caccttttgt gattgcaaac cgtgctatgg
    22081 aaaaaatgtc tggaagccgt actatcaaca ttgtgatcac aaaaacaggt gaagaagaac
    22141 cttggaaaaa tccggataac ttgaaagtcg ttgaagaatt tcaggaattt ttagcaaatg
    22201 aaccaagggt caagcggaca ctctcgcttg ttgatttgat taagcgtatc agctatgctt
    22261 ttaatgagaa tcgcaaagaa tttaatcgac taccgaacgg atttgaattt ctggaaagtg
    22321 aggagatatt tgaagaaaac ggccaaaccg aaaagcgaac tgtaaagaga gaaatcagtg
    22381 ggcgggattt gatagcacag tatcttgtgc tctatgaaaa ttccggaggt gatgtacttt
    22441 ccgacgtggt tgacagcgag tatctaaatt taaatttggc agttactatc agttctaatt
    22501 caactacgga agaggaaaaa cttctgcaga gtattgaaga ttatgcggtc cggcagtttc
    22561 cgcagggttt tagcatgact tcatcaggaa tggtgccgat aaatgttgca acaagcaatg
    22621 aagtagtgac tagtcaaatc cgaagtttga gcggttctat attagctgta tttttaatgc
    22681 tggctttgat ttttcgctca gtttcaaggg gattaatggg aatgattccc cttgtcttta
    22741 cggtgctgtt taattttgga gtaatggggt tttttggtgt tcatctggac ataggtaccg
    22801 cccttgtcag cagtattgtg attggaattg gtgttgatta cagtattcac tacctatcca
    22861 gcatgtttca cgaattagct gatggtacag aactacatga tgcaatatca aacacggtca
    22921 gacgcagtgg aaaagccatc acgtctaatg cagtaactgt aggttttggt tttttggctt
    22981 tgagtgtctc agaattttta ccattagtaa cactgagctg gatgatttgg ctaacattaa
    23041 atatcagtgc gttggcaacc atgattttaa ttccggcact tgccgtgaca ttgacggatg
    23101 ttttgaaact agtgccgtca cagaatgatt aattagagct tgatcaaaaa atataatcaa
    23161 ttgtaataat agttatttac ttgtagtttc tgaaaaagat ttaataaaaa tctgtcagtt
    23221 ttcagtcgtc tgctatcagt attttgggtc gaattataaa tcggatagct tatagctgat
    23281 tctctgctga ttcaaaacct gctgaaacca taataaaaac tggacgagac agattcacgt
    23341 tcagagatta attaacaaaa ttattattcc attcatgtca aattcattac gtatatttca
    23401 tcagttacaa gccgatttaa atcaaatcat tttaggacag gaacgcctga tagaacgctt
    23461 attgatagta ctgttagcag acggacatct cttagttgaa ggtgcacccg gtctggcaaa
    23521 aacccgtgca attaaagctc ttggtgagaa aattgaggga gaattccaac gtatacagtt
    23581 cactccggat ttattacctt ccgatgtaac agggacggac atatatcgtc cccaagaggg
    23641 tagttttcac tttcagtcag gaccggtttt tcataattta gtcttggcag atgaaattaa
    23701 tcgtgctccc gcaaaggtcc aatcggctct tctggaagct atgggcgaaa ggcaggtaac
    23761 ggttggtgga caaacgtatc ctcttccgga tttgttcctg gtgatggcta cacaaaatcc
    23821 tattgaacag gaagggacgt atcctcttcc tgaagcgcag cttgaccgtt ttttacttca
    23881 cgtatttata gactatccct caatagaaaa tgaattaaaa ataatgcgcc ttgtccgaaa
    23941 agaagctttg agtgaaattc aaatttcatc ggataaaagt ccgaaatctg cggttgattc
    24001 aggaacaaaa tctgcaggac gaaaaatttc tgcagtacat atttttgaag caagaaatga
    24061 agcactgaat atatacatgg cgcctgaaat agaacgttat ttggttgaga ttattgcagc
    24121 cacaaggatt ccggaaaaat atagcgacga gttggcacgt ttgatcacct ggggtgccag
    24181 tcctagaggg gcgctgtcac ttgaccggtg cgcccgtgcc cgtgcctggc tgcatgaacg
    24241 tgattttgtc actccggaag atttgcagga tttaagcggt gacgtgctgc gtcacagaat
    24301 tattgaaagt tatgaagctg aatctgatgg tattaccacc gatgacgtga ttgcaaaaat
    24361 tcttgctcag gtggccgttc cttaaaaatt gcttctgtgc ttaatcttgc atgaatatgg
    24421 taaattcagg agattatttt caataacagg caggttttaa attctccaaa ctaatgcttc
    24481 actacattgc attcaattac cagaattaaa taatcaaaaa atatcaaaac agattgcatt
    24541 ttattatatt tgtaattggc aattcaaaac aattatttgg ctttcattta gaatatgatt
    24601 tattcacaaa taagcagaca gaacattgaa caatgaaata tcttgaccca acggcagtgg
    24661 ttctgttttt tgttgtttct ctggcaggca tagttcaaca ttcctttttg cttcagttga
    24721 attctgattg gagtgagcgg ttgttccaag cctatgcact tggtgtgggt tatgacgtga
    24781 tgaacggagc tattttcagc acattagctg tgatttctcc ttttccaatc ttatttagga
    24841 aaattttctt cagcattctt ggtcttggat tatttgcttt cctgttcaca gattaccatt
    24901 acgttttgat ttttggtacc caccttcctt tttctaccat tgaatatttg aatgatagcg
    24961 gtgcattttt gtcatccgca gcacatgcag taaatgacat aactttttgg attttattcg
    25021 ttttcccttc ggtgatgctg gtgtttatgc tttggcgttt tgggaaaaaa cggaacagtt
    25081 ggaaagaaga ttttaagtgc agggcatcaa cgctcctgtt tataatttta attggggggc
    25141 ctgctgcaag ttacagtaat tcctatgttt caaaaaacat ggaaaatccg cttacatctg
    25201 cagcattgca atatttttat tattccaaag atcgtgaacc tgataaaaaa attcttcgtc
    25261 ctgaaaaatc tctaaaaata gtggagacat taattcaagg taaaaaacct tcaggaaaaa
    25321 tatgggataa ttatccgctc gtgcgaatac gtgaggcaac aggatgtact ggacagaatc
    25381 attctgagct tactaaaatt ctgtgtacaa ctgcagacag acctaatatt ttaatattgt
    25441 tcctggagtc atttagggct gcggaagtag gtgtttacgg cagcaagttg gggcttactc
    25501 cggagtttga tcgctggtcc aagcacggaa tattatataa aaactttttc gcaaacggtt
    25561 ttcaaaccag acatggacag gtggccacct actgttcttt gtttccaaat tacggtgctg
    25621 ctgttatgaa gcgctacacc gggaacagct tcatgtgtct tccggaatac ctaaaaaatt
    25681 caggttatta cacctcatgg gtttacggtt ctgatgcaaa ctttgacgga cagtcaacat
    25741 ttttgccaaa agtcggcttt gataaaattg tagatgaatt tgattttcca aacgaggccg
    25801 tgacacttgg ctgggggctt tctgatgaag acctcttccg gaaatgggaa actgttttag
    25861 acagtgagcc ggaaccattt ttctcttcag ctttaactac aaccaatcac catccttttg
    25921 aagtcccgga taattatcgg cttcaagcgg gagattcagt gacagaccgc taccgagaat
    25981 ctttacacta tactgatgcc atgttggggg gctttcttaa aaggatttcc aaaaaaccgt
    26041 ggtttaaaaa taccattatt tttgtgactg cagatacttc cagttatcaa aatgcggaac
    26101 agcataccaa caattatgaa gaatttgtta atctgcattc tcaagttccg ttacttattt
    26161 tatcaggtaa atccaccgcc ctaggaaaaa aatctgaggg aattattatc actcaacatg
    26221 ccagtcaagt ggatttggca cctacaattt tggatatttt acgtaaaaaa tacatagttc
    26281 cctgggttgg gcgctcattg ttgaattcag ttgatttgct gactgatgtg cctcaacgtg
    26341 catttacaaa tcgtcctgga gcatattggg cggtcattga agaaaaatca cgttattatc
    26401 gtgaaaatga ccagcgagat catttttttg gagatcagga caatcaaaaa ggactgcact
    26461 tgaaggaaat tggatcatcc tggattgaaa ctattcactg ggttttgcag gaaaaccgag
    26521 tatggccgga aaaataaaaa aatgaaagat gctgctgaaa aaaatggaat atttcttgga
    26581 aaaatggagt tgccggaatt ggttaggatt gcccataaga aaggactgtt gttgactgca
    26641 gaaaatgttg gatcacttgg aggtccatat ttgactaaac tcaagggaag agggatggag
    26701 tttgctgaag cccgctctta tcagtacgga gatgatgtgc gtcatattga ctggagggtg
    26761 acggcacgaa cgggcacgac acacactaag ttgttcaggg aagaacgtga gcgtccagtg
    26821 atgctggttt tggatctgtc ccgccggatg ttttttggaa ctcgccaaag attaaaatca
    26881 gttcaagcag cccgtattgc cgctttgacg ggttggaggg cacttctacg gggagatcga
    26941 gttggaggtg ttatatattc agaagaaatg catcgggaat tcaaaccaag aagtgaccgt
    27001 cgacattttt tacgtttgct ttcaaatatt atgactgagc ataatcgaat tgcagacagg
    27061 atgaataata atgaatggag ttttggtagc aattctcttt ttgcccaggc attgaggcgt
    27121 gtgcgacaag tagtacatcc gggaagtttg atttacatat tcagtgattt ttctggattt
    27181 aatgcagaat gccggaagca cttgtttcaa ctttccaaac aaaatcagct ggctgccttt
    27241 ttaatttcag atcctctgga aaaagaagca ccatcttccg gacgttatgc aatgagtgac
    27301 ggtgaggctg ttgcctgggt taactccgaa aattcaaaaa cacgtcaact ctatgcttcc
    27361 acttatgaag atcagctaaa aagtttaaag cacgaattac aactctgcag gctaagcttg
    27421 aaaatgctga gtacagtgga caatgtttca cagatttaat gaatcaatca ttttgattac
    27481 aaacaatttc aaatgccgga agaaaatcct ctaataaatt taaaagacat tcacttacca
    27541 ccagctgttg caatttggcc tccggctcca ggctggtgga ttgtggcagt agtactgctt
    27601 ttgatttttg tgttttgcgg aatgtggatc agtcgtcgtt ttgaacggcg aaaacccaaa
    27661 actgaagctc tgcgattgtt aaaaaatctg caaaatcaac aaaataatac cgaagaatca
    27721 ttggaaatac tcagggattt atcgcaatta ctgcgtcggg ttgcattgac tttttgtgca
    27781 gatgaaaatg tagcttcact gcatggatcc gaatggttgg agtttcttga tagaacagga
    27841 aaaaccactg aatttactca aggagcaggg catgtatttg gaaaggaact ttatcagcaa
    27901 aatccggaaa ttgaaataga tgttttgttt ccaatggtaa aaaaatggat tatggattgt
    27961 agtcagcagc attgaacaaa gcacggaaaa tgtttttatt tgagaatggt gtgtttgaat
    28021 ttatttggct ttgggtggcg tggttctggc cactgccctt attgtttcgt gttcttccac
    28081 cagttgatag gtcagttcag gctcttcgtg tacctttttt tcatagaatt gcagtttttc
    28141 ctaaatccgg aatttcagca ggaaaaaact caaaagtctt gctgttagcc ggattggtct
    28201 ggagtttgat tgttctggca gcactgcggc cgcaatgggt tggggatcca gtagaattac
    28261 ctgtttccgg ccgaagtgtg atgcttgcgg ttgatctttc cggaagtatg aaagaaacgg
    28321 atctggaact gcacggtgaa tccgtgacgc ggctggctgt tgtaaaatca gttttgaggc
    28381 cttttattga gcggagaaaa ggcgaccggc tggggctgat tctgtttgga gatcaggctt
    28441 atctgcaaac tccactgact ttcgaccgga aaacattgcg gcaaatgctt gaagagtctg
    28501 aattaggtct tgccggacag agaactgcaa ttggtaatgc aatcggactg gccgtaaaac
    28561 gtatgaaaga tcttccggat aaagaaaaag tattggtgtt gctcacagat ggtcaaaata
    28621 ccgccggtga ggttactcct gaagatgcag ggcgtttagc taaagaaacg gggctacgga
    28681 tccacaccat tggtattggt gcagatgagg catggataag ggcttttttc ggcaagcaaa
    28741 aaattaatcc atctgcagaa cttgatgaag caatgctgaa atctttagca agccagactg
    28801 gtggtacata ttttcgggcg agaagtacag aagaactgga aaaaatttat gcaatgattg
    28861 ataaaattga accgttggaa atagaaaaag aggttttccg tccccgtcaa gccttgttta
    28921 tgtggccgct gggttttgca gtactgatag ttggaatatg gtcagccgtt ttattgatta
    28981 gaaactaatc atatttatta tgttaagttt cgtaattgat ctaaaaagaa agaaatattt
    29041 agccgtcatt gatcagataa tttataaaga attttctgac tgaaaacaaa gagctgaata
    29101 ataagtgcat ctgttattgt tcatctcggg agtcatgaat ttttgtaaga tactaatagt
    29161 attgactgcg gtatgtcatc tcgactgaag ggagagatct gataaagtac tgatttctta
    29221 taagatacct cacatacgtt tgggatgaca cgctaaaact cgtgattgtc tagttattca
    29281 ttcaaagcaa gtataaatca tgaacaaccc cggctcaggt gaattttcat taaagcacca
    29341 gcctaatatt cgttaattat tttgttatca gaatttcaat ttttacgccc tctgtggcta
    29401 atagcaatac cagttctctg gtggatggtt tttcgtttcc ttggaagatg gtggagcaaa
    29461 ggaaactggg aagatgtggt agaaccgcat ttgctgtcgt ttcttgcaac aactccagca
    29521 acgcaaaaac ggagtctgct gccgtggatc atctgtttag tcggtactat tcttttgctg
    29581 gctttgtccg gacctgtctg gcaaaagaat ctacagccgc tgctgcaaaa atcactggcc
    29641 agagtactgg ttttggattt atcttattcc atgctggcaa cagacataaa accaacacgg
    29701 tttgagcgag cacgtttcaa attgaaagac ctgctaggac attttgttga aggtgaaact
    29761 gcacttatcg tatatgcagg agatgcattc gttatcagcc cattgaccca tgatcctgtc
    29821 accattgcgt ctcttcttac ggggcttcat ccgaatatta tgcctcttcc cggaagtcgt
    29881 tcagatcttg caattgaact tgctgcagat cttttgagcc gaaggaatgg cggtactgga
    29941 catatcatct gggttacaga cggaattgaa gtacaggata ttccagcagc agaaaatgcg
    30001 gttggtttga atcgcctgtc aattcttgca gttggaactg aatccggctc accgatcccc
    30061 ttgtccggag gagggtttct taaagacaaa aaagggggaa ttgtaatacc aaaactttat
    30121 ttaaaacctt tacaaaaatt agccgctcaa actcatggtg ccttcacaaa attgagtccg
    30181 gatgatcggg atgtggagca aatcattgca gcagaaactg ctgcagatga ttatgttgaa
    30241 gacaaacagc agagaacttc tgataagtgg aatgaagaag gaccatggct cttattactt
    30301 gtcttgccca tggcagcgtt gctgtttcgt aggggtctat tgttttcaat tggatttgca
    30361 gtattacttg gagtcacagc aaggccttct tcagcgctcg cattcggatg ggatgattta
    30421 tggttacgac cggaccagca ggcagctaaa ttgtttcagc aaggtgaaac gaaacaagca
    30481 gctgaacttt ttgaaagctc ggaatggaaa ggggcagcgg catatcgatc tggcgactat
    30541 gaaaaagcaa ttgagcattt ttctcaacag aatcattcac gggccaattt caacagtgga
    30601 aatgcattgg cctttgcagg acggttacag gaatcactgg aagcttttga gcgggtgtta
    30661 gcagatagtc cgcaagacgt agatgctcag tacaatcatg atttgattga aaaactacta
    30721 aaagaacaac aaaagaaaaa acaacagcag gagggacaac agggtgcaaa tcaaaaaaat
    30781 aaacaggatc aacaggatgg ctctagccaa actcaggaaa aacaaaatgc acagtccgcc
    30841 caggattcgt ctggtgacaa tgcgggtacc agtgatcagc agacagcaga gggcgtaaaa
    30901 aataatgctg aaaaggaatc aactgcagag aaaaataatg aaaaatttga tgaagaaaaa
    30961 actgaatctc caaataatga agaagggcag aaaaaaattg atggatctgc cgaaaaagaa
    31021 aacgcaaaaa acaagaaaca ggattcaact ccggttgatc ttgccaaaca gcaattcctt
    31081 gaacaatggt tgagaaaaat acctgatgat cccgggcgct tgttacgcaa caaaatggaa
    31141 agggaatttc aacgccgcgg aaaacaaaat tttacaaatg aacaatattg gtaaatgatg
    31201 ataaaactat ttattttggg agtcttgata ttggccggta atccagcctg gggcgatgtt
    31261 actgcatggg taaataaaaa tccggtagtg gtaggagaca tgttccagtt acatgtagaa
    31321 gcaaaaaatg ttgatgatgc cgaggaacca gatttaagcg gaattaccgg tttgcaaatt
    31381 ttgaatcgaa gtgtgcaaaa tcgaacctcc atcgttggga cttctatcac aagaactttg
    31441 agctggacct atattatgat tgctccatct tccggagatt acctgatacc agccctccag
    31501 gtaggaaatg agcaaagcac tcccattgca ttaaaagtga ctgcatactc acagaatcag
    31561 cagcagaaat atgttagtct tgacgtggag gttgttccca aaaaagttta tccacagcag
    31621 cagattctcg tccgattaag gatttctcga actggagtac aactggaaaa tgaaacgatt
    31681 acaccgtttg aactgaagga agcactagta gaggagctaa gtcaaaaatc ctaccagact
    31741 gtcaaaagtg ggaaaaaaca taagattact gaaattttat atgcagtgat tccggaaaaa
    31801 agcgggacac ttgttattcc acaggttcgt tatcagggtg aaataattca aagaggtcca
    31861 tcacaaaata attttggtaa ttttggcaat ctttttcaaa aacggggacg aagaatattt
    31921 agtacaagta caacgcaatc agttgaagtt aagccgctgc cgtcaggttt caagaactgg
    31981 tggctgccga cagacaaact tgtgatagaa gaaaagtggc agccggatcc tccggtattt
    32041 agagtaggag aaccggtgac tagaactgtt gcgatctttg caaatggtgt ttctgggaat
    32101 caaattccgg aattaaaatt tgattatcct gttactttga agggatatgc agatcaacct
    32161 ttaattgaga cagaaaaaac ggaaaaggga cttaaaggga tgcgattgga aaaatgggct
    32221 cttattccaa gtcatccagg aaaaattgaa cttccgggag tttctgtcag ttggtgggac
    32281 attaaaagtg atacgcttca gactgcagtc atatctccaa aaatatttga tatacttcct
    32341 gtaccagaaa atttacagga aatacctgaa aaggaaactc ctgaattaca gtcaaatcag
    32401 gcagcaacta caaaagttct gcaggaaaaa gagacggcaa tagaatcatt cccttggaag
    32461 atattggcct tcggtttagc atttctttgg tgcgcaacaa tgttgatgtg gtttataaat
    32521 aaaaataata aagctgctgc tgtcactaaa aataaaaaca aatttataca ggatcggaag
    32581 aacgctctta gagaagcaac tagaaatgca gaaaaagctt ttagttccgg agatcccgga
    32641 attgtgcaaa cggcactttt aaaatggggt actgcagttt ggactgatga tcctccgcaa
    32701 ggtttagaac aaattgggga aagaatgccg gaattaaaga atggaataaa tgatctaaat
    32761 tctgtactct acggaaataa tcaaacaaaa gaaagttcat tggagaattt atttaatgat
    32821 tttctcaagg tttctttatt agataaaaaa tttaataata acaaaggaca gtcacagttg
    32881 gaacctttat atccggagta gatatgattg aatatttgag cgccttttta aacgcttatt
    32941 tgacagaaaa tttattagaa ttaggattga caacaggcat ggttgaatcg ctggtgcgcg
    33001 gaggattaat tttgctggta cttttgttga gctgggttgc tcatagagtt tcacagggac
    33061 ctataaatag gtcaattgag aaatttgcac actataccat tcagcaatgg gacgatatcc
    33121 tggttgaaaa acatattgtt aaacgtatac tttactttat tccactaata ctgctttatg
    33181 tactgtcctc accaatattg acaggaacat cactgctgcc gttgagtcag acattgatca
    33241 gtgttttgtt tttgattgcc ggaatgatgt ttttggatgc aatactgagc gcactgttgg
    33301 caatttacgg caaatctgca attgcaaaag aaatttcgat tattcctttt gtgcaggtac
    33361 ttaagttggg gctgtatttt gtaacaggaa ttttgctgtt gtctttgctg cttcaaaaaa
    33421 ctccgctcta ctttttaagt ggtttaggag ccttaactgc agtattgatg tttgtattca
    33481 aagatgtgct gatgggattt gttgcaggaa ttcagttaat tgctaataaa atggtggctc
    33541 cgaaggattg gattgaaatg cctaaatatg gggcagatgg agatgtgatt gaaattacac
    33601 tcactactgt caaagtccaa aattttgaca atactatcac aactattcca acttatgcac
    33661 ttatcaatga gtcatttaaa aattggcgga atatgaattt atccggaggt agaagaatta
    33721 aaagatatgt aaatattgac ctcggcagca taaagttttg cagcagtgaa atgctggaac
    33781 aatttaaacg tattcaattg atcagtcagt atattcaaaa ccggcaagaa gaaatattgg
    33841 tttataataa gaaacaccag gttgacgaaa gcacactggt taatggcaga agattaacaa
    33901 atatcggagt atttcgctca tacgttgaag catatttaag acagcatccc atgatacata
    33961 aggatatgac tttcctcata cgtcagcttt caccaagtga gaatggattg ccgattgaaa
    34021 tatatgtttt ttgtaaagac actaactgga ctgcctacga agcaatacag gctgatatat
    34081 ttgatcatat tttggctgta gttcccgaat ttgatttacg tgtctttcaa gaaccatcag
    34141 gctcagattt tcaaaaaata gcactgtagt ta
//
LOCUS       DTVD01000041            2913 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13814_length_2913_cov_178.888383, whole genome
            shotgun sequence.
ACCESSION   DTVD01000041 DTVD01000000
VERSION     DTVD01000041.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13814_length_2913_cov_178.888383"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="EYO68_01505"
     CDS             complement(<1..1233)
                     /locus_tag="EYO68_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06510.1"
                     /translation="MNSPPAGIALLVQWQAGDDTAQKKLLTLFDAVIEGEFDAVFQES
                     AKSDTVCVTTSLHLMTLTMLNQLYGLNSKEFYKGDPERYVRTTLMTQRLLGIRKLTLG
                     WPVYAFGAEALGQATMYPDQHAPGADPGDSLVDRNNWYKLETPDFEGEVPRVIEAMLA
                     SFAELTGLEPVAHLPAPYSLAADIFGQETLITALSHEPDFVVEFLEHLTEKIIVPWCE
                     RLVQKFPNIWLELSDASGSPLFISPHLFQKIAAGPVLRLIQEYPWGSRVFVSNYRGDM
                     TPKTSRVEGRRGAGRRNRRGQQTDPERSNAQSSTEALNELIDFKLSLCTEFIIKLDAD
                     QSPLSIYIEQAIRREKPLYLGIGATRIDRNSIVDHEAAKSELEELASNSAKAIKAVSE
                     SLADKGQYRSNLSWPGDVY"
     gene            complement(1233..2684)
                     /locus_tag="EYO68_01510"
     CDS             complement(1233..2684)
                     /locus_tag="EYO68_01510"
                     /inference="COORDINATES: protein motif:HMM:PF10223.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2181 domain-containing protein"
                     /protein_id="HIC06511.1"
                     /translation="MKALSLHYGYMKEQYPDKDLMLIFDIDGSINDMQYQLFRALQTF
                     DQLQGTHYFYRLMPDEIKIHEDLLSSFLTEYQLAPAQKEAFLSWYTSYRKSSRVQLRP
                     MEGVIETICWFQMQSRTAVGLKNICSQDMQKKTIQALRKFGKEYQLKLPKELIYFPKH
                     HAPSESAVESLSYFRKIGYHVICFVDSETQSLDAIANEKDTRDILLLQADTVYRSNDK
                     PMTAGVEKGSTYELRELVNEKDLPPTIDLVWHGINDRKNLRQFLSSNIQWGEVDVQTV
                     HEDGSVLLQHDNPLEEETLLTGKINLQLNELLVEFQQHGKAVKVDFKDKSNVLSESIK
                     LLKKHGFNDQNIWLNGNIDVLKEDGFKMLSAAFPNAVIQTSVDFICPLVFSLPDQAKK
                     ILTQLKGWGINRFSINWEMKKCSALMYQLKKWGFEVNVYNATDLEQFLKVMILSPRSI
                     TSDFNFPEWNYYGRGSGKNGIQSDYVEQQVKFK"
     gene            complement(2793..>2913)
                     /locus_tag="EYO68_01515"
     CDS             complement(2793..>2913)
                     /locus_tag="EYO68_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06512.1"
                     /translation="NLLRSIQSANVSPLSPGTKVDWGVNPEQLFFFDNSGKRL"
BASE COUNT          814 a          657 c          607 g          835 t
ORIGIN      
        1 gtacacatcc cccggccaag aaagatttga gcgatattga cccttatcag ctaaagactc
       61 tgaaacagct ttgatagctt tagccgaatt tgacgccaat tcttcaagtt ctgatttagc
      121 tgcttcatga tcaacaatgc tgttccggtc gatgcgagta gctccaattc ccaaataaag
      181 cggcttttca cgacgaatcg cctgttcgat ataaatcgaa agaggagatt gatcagcatc
      241 cagtttgatg atgaactcag tacagagact cagtttgaaa tcaatcaatt cgttcaaagc
      301 ctcggttgag gattgagcgt tgctccgttc cggatctgtt tgttgccctc gacgattacg
      361 ccgccctgct ccccggcgcc cttccacgcg ggaggttttt ggggtcatgt cacctcgata
      421 gttggagaca aacacccgac ttccccaagg atattcctga atcagtcgaa ggactggccc
      481 ggccgcgatt ttttgaaaaa gatgtggact tatgaataaa ggagaacccg aagcatctga
      541 aagttcaagc catatattcg gaaatttttg cactaaacgt tcgcaccagg gaacgattat
      601 tttttcagtt aaatgttcca gaaattcaac cacaaaatcc ggctcatggc ttaaagcggt
      661 aatcaaagtt tcctgtccaa aaatatccgc ggcaaggctg taaggcgcag gcaaatgagc
      721 aacaggttca agtcctgtca gttctgcaaa agaagccaac attgcttcga taactcgcgg
      781 aacttctccc tcaaaatccg gagtttccag tttataccag ttattccggt caaccagaga
      841 atctcccgga tcagcaccgg gggcatgttg atccggatac atggtggcct gtcccagagc
      901 ttctgcgcca aaagcataaa ccggccagcc gagagtcaat ttgcggatcc cgagtaaacg
      961 ctgagtcatg agtgtcgtcc ggacataacg ctctgggtcg cctttgtaaa actcttttga
     1021 gttgagaccg taaagctgat tcagcatggt cagcgtcatc aaatgtagag aggtggtcac
     1081 gcagactgta tctgacttag ccgattcttg aaatacagca tcgaattctc cttcaatcac
     1141 cgcatcaaac agagttagta gttttttttg tgcggtatca tctccagcct gccactggac
     1201 aagcagagca atgcctgcag gtggactatt cattatttga atttcacctg ctgttccaca
     1261 taatccgatt ggatgccatt tttaccggaa ccacgaccgt aataattcca ttctggaaaa
     1321 ttaaagtccg aagtgattga gcgtggagac agaatcatca ccttaagaaa ctgttctaaa
     1381 tcagtcgcgt tatagacatt tacttcaaat ccccattttt taagctgata catcaaggct
     1441 gaacattttt tcatttccca gttgatggaa aaacgattaa tgccccagcc ttttaattgc
     1501 gtgagtattt tcttggcttg gtctggcagg gagaagacca agggacagat aaaatcgacg
     1561 gaggtttgta tgacagcatt gggaaaggct gctgacaaca ttttaaaccc atcttcttta
     1621 agcacatcga tattaccatt cagccaaata ttttgatcat taaaaccatg ctttttgagt
     1681 agctttattg attcactcag aacattgctc ttatctttga aatcaacttt gactgccttg
     1741 ccatgttgtt gaaactcgac caataactcg tttaattgca gatttatctt tccggtcaat
     1801 aacgtctcct cttccaacgg attgtcgtgt tgcagtaaca ctgatccatc ttcatgcacg
     1861 gtctgaacat cgacctcgcc ccattgtatg ttagatgaga gaaactgccg caaatttttc
     1921 cggtcattga ttccatgcca aaccaagtca atggtgggag gtaagtcctt ttcatttacc
     1981 agttctcgca attcataggt actgcctttt tccacacctg cggtcatcgg cttatcattt
     2041 gagcgataaa cagtatccgc ttggagcagt aaaatatcgc gcgtatcttt ttcgtttgca
     2101 atggcatcaa gtgactgcgt ttcactgtca acaaaacaaa tcacatgata accgattttg
     2161 cgaaaataag agagactttc tactgctgac tcagacggtg catgatgctt ggggaagtaa
     2221 atcaattctt taggtagttt taactgatat tctttaccaa attttctcag tgcctggatg
     2281 gtttttttct gcatatcctg ggagcaaata tttttcaatc cgaccgccgt tctggactgc
     2341 atctgaaacc aacaaatggt ttcaatcacc ccttccatcg gtcgcaattg tacccgtgaa
     2401 gacttacggt aactcgtgta ccaagagaga aaggcttcct tttgcgcggg tgccaattga
     2461 tattcagtga ggaatgaaga cagcaaatcc tcatgaattt tgatttcatc gggcattaag
     2521 cggtaaaaat aatgagtgcc ttgtagttgg tcaaaggtct gcaatgctcg aaaaagctga
     2581 tattgcatat cgttaataga accgtcgata tcaaatatca acatcaaatc cttatctgga
     2641 tactgttctt tcatgtaacc ataatgcaga cttaatgctt tcatccagtt attcaagagg
     2701 acctcttttc ataaactcga agaaacgatt ttggcctaaa ccctgcaatt tacaatactt
     2761 ttcagcataa gttttgagcg actgtagagg tctcataagc gtttgccgga attgtcgaaa
     2821 aagaaaagtt gttcagggtt aactccccaa tccacttttg ttcccggaga gagaggagaa
     2881 acattagcgg attggatcga acgcaggagg tta
//
LOCUS       DTVD01000042            2895 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_13936_length_2895_cov_67.340141, whole genome
            shotgun sequence.
ACCESSION   DTVD01000042 DTVD01000000
VERSION     DTVD01000042.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13936_length_2895_cov_67.340141"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="EYO68_01520"
     CDS             complement(<1..205)
                     /locus_tag="EYO68_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06513.1"
                     /translation="MKTLIANIEKQFEKLGYFIFARRWTVILVALLIFGALASQVTNI
                     VIDTSNEAFLEPDDPILTQYDAFR"
     gene            complement(244..768)
                     /locus_tag="EYO68_01525"
     CDS             complement(244..768)
                     /locus_tag="EYO68_01525"
                     /inference="COORDINATES: protein motif:HMM:PF01292.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIC06514.1"
                     /translation="MLKKYSNKLVTLHWLTVPLVIFSLVMGILVLAELPNNAEKINSL
                     RVHMLVGFVIGLLTLIRIFVKKKHEPLEPLKIDNPVRKKLIKVTHFSLYTLLLLMVSS
                     GVSLSLISGVGEIAFFGSTAALPEDFEVFLPKTAHAIFAKVLFAFIGILIVGVLLYKF
                     KTDSSISKRMWFGK"
     gene            complement(775..1341)
                     /locus_tag="EYO68_01530"
     CDS             complement(775..1341)
                     /locus_tag="EYO68_01530"
                     /inference="COORDINATES: protein motif:HMM:PF00440.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIC06515.1"
                     /translation="MIQDTRKKLLEGTRRCLVEKGFQGTTVKAIAKYAGVNHGLVHHY
                     FGSKEELLAALVEKVTEGEGTPMSSNSKNGVHQYDDVSAMSSAEMETFAKKKLVPHMT
                     SDYNKFVIELLVMSEEMPSVAKQVCKLLNRRREWFGRILGVEDSSLLLLISGAMMGLM
                     LQYKLDPTINIEDAVIRLFSLISKGKSR"
     gene            1625..1921
                     /locus_tag="EYO68_01535"
     CDS             1625..1921
                     /locus_tag="EYO68_01535"
                     /inference="COORDINATES: protein motif:HMM:PF00665.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE domain-containing protein"
                     /protein_id="HIC06516.1"
                     /translation="MTITEPDQVWCSDITYIRLAHGFVYLTAVMDWASRYVLSWEVSV
                     TMDDDFCVNALKSALRRHKTPEIFNTDQGAQYQSLVGKTPAEIYWGREVAKKAA"
     gene            2093..2368
                     /locus_tag="EYO68_01540"
     CDS             2093..2368
                     /locus_tag="EYO68_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIC06517.1"
                     /translation="MVTALVLLNIERQHIKDISQKLVNMPEVSEVYSVAGRYDLVAIV
                     RVKTNEQIAETIAEKFGELNGITASETLIAFNTFSRHDLELMFSIGE"
     gene            2375..>2895
                     /locus_tag="EYO68_01545"
     CDS             2375..>2895
                     /locus_tag="EYO68_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC06518.1"
                     /translation="MPDSIPQQSIKVRDPIFRMSFYDPPLENRNPEEYLLMDFNEPPL
                     PPPAEVIDKIAILLKNRIHAYPIYGDFLERLSEYAAVPTENLLLTNGSDQGIDVILRS
                     LLESGDEVAMVQPGFPFFRQVAGTIGCSVVGPQFPPDFRFPHEELKDSVKRQTRLIVV
                     INPNNPTGSAVSL"
BASE COUNT          849 a          613 c          558 g          875 t
ORIGIN      
        1 cacggaatgc gtcatactga gtcagtatcg gatcatccgg ttccagaaat gcttcattgg
       61 atgtatcgat tactatattg gttacctgtg aagcgagggc tccaaatatg agcaaagcca
      121 ccagaatcac tgtccagcgc ctggcgaaga taaagtagcc aagtttttca aattgttttt
      181 cgatatttgc aatgagtgtt ttcatgtttt catctttggt tattgactta atttaataat
      241 aggttatttt ccaaaccaca ttcttttgga aatactggaa tccgttttga atttataaag
      301 caggacaccc acgataagaa tgccgatgaa tgcaaacaga actttggcaa aaattgcatg
      361 tgcagttttc ggcaggaaaa cctcaaaatc ttccggtagt gctgcagtcg agccaaagaa
      421 agctatttct ccaacgcccg aaattaacga aaggctaact ccactggaaa ccatcaataa
      481 aagcagtgta taaagcgaaa aatgggtaac tttaatgagc ttcttccgca ctggattatc
      541 tattttcaaa ggttcaagcg gttcgtgctt cttttttaca aaaattctaa ttagtgtcag
      601 caggccgatt acaaagccga caagcatgtg tacacgtaat gaatttattt tctccgcatt
      661 attcggtaat tcagccaaaa ctaagatacc catcaccagg ctgaaaatca ccagtggaac
      721 tgtcaaccag tgtaatgtaa ccagtttatt actatatttt ttcaacattt ttttctacct
      781 gctttttcct tttgaaatta aactaaataa gcgaattact gcatcttcaa tgttaatggt
      841 tgggtccagt ttatattgca gcatcaagcc catcatggca ccactgatta acaagagcag
      901 gcttgaatct tctactccca aaatccgtcc aaaccattca cgcctgcgat tcaacaactt
      961 gcagacctgt tttgctacac ttggcatttc ctcactcatg accagcaatt ctatcacaaa
     1021 cttgttgtaa tccgaggtca tgtgaggaac aagcttcttt tttgcaaagg tctccatttc
     1081 tgctgaactc atggcagaga catcatcgta ctgatgcacc ccattttttg agttcgaaga
     1141 cattggagta ccttcgccct ccgtaacttt ttcgaccaaa gcggccagta gttcttcttt
     1201 agaaccaaaa taatgatgca ccagaccatg atttaccccg gcatattttg caatagcttt
     1261 taccgtggtt ccctgaaaac ctttctccac aaggcacctg cgtgtacctt ccaacagctt
     1321 ttttctagta tcttgaatca taattatgat ttagttgaac gactaacttt cgaactggtc
     1381 tagtcgtttg actaatattt gtcaagtttt tcttccgttt ttccgaattt tctcaattat
     1441 taccgaaagc aatttcagtt ttattttatt gctactttac gcttcatcct tgaagcgcgt
     1501 cctccgtagt tcatggctga tattggatat aatgtgtttt ttattacagt aaatactatt
     1561 gtgtaaaatc aattaatagt cttcaaaaga ggagaataag gtttatcctt atctgctgaa
     1621 aaggatgact attacagaac ctgatcaagt gtggtgctca gatatcactt acattcggct
     1681 ggcacacggc tttgtttatc tgacagccgt aatggattgg gccagtcgct atgtgctgtc
     1741 ctgggaagtc tctgtgacca tggatgatga tttctgtgtt aatgccttga agagtgccct
     1801 gcgcaggcat aagacacctg agatattcaa tacagaccag ggagcacaat atcaatctct
     1861 ggtaggaaaa actcctgcag agatttattg gggtagagaa gttgccaaga aggcggcatg
     1921 aataatacaa tgaaaaacaa actaaaatac accttaactc atgagagaaa ctgtccttga
     1981 cttggggtcc acttcaagta acataactac agacaaagcc cttttgaaaa tcattgagct
     2041 ttgttacaat aaaaaacctc tcagcaaaca acccacaaca caggagaaaa tcatggttac
     2101 agcactggta cttttgaata ttgaacgaca gcacataaag gacatttccc aaaaactggt
     2161 caatatgcca gaagtttcgg aagtctattc tgtcgctggt cgttatgatt tggtggcaat
     2221 tgtgagggtc aaaaccaatg agcaaattgc ggaaacaatt gccgaaaaat ttggagaact
     2281 gaatggaatc actgcctcag aaacattaat cgccttcaat actttttcgc gacacgatct
     2341 tgaacttatg ttttcaatcg gagagtgaga acacgtgcca gattcaattc cgcagcaaag
     2401 tattaaagtc cgggatccaa ttttccggat gagtttttac gatccacctc ttgagaatcg
     2461 taatccggaa gaatatttgt tgatggattt caacgaaccg cctttacctc ctcctgcgga
     2521 agttattgat aagattgcca ttttactaaa aaatcgaatt cacgcatatc ccatatatgg
     2581 tgattttctg gagaggttga gcgaatatgc cgcggttcca acagaaaatt tactgctgac
     2641 aaacggctcg gatcagggga ttgacgtcat tttacgcagt ttattggaaa gcggcgatga
     2701 agttgcaatg gttcagcctg gtttcccctt tttccgccag gttgcaggta caatcggctg
     2761 ttcggttgtc ggtcctcaat ttccgccgga ctttcgtttt ccccatgaag aattaaaaga
     2821 ttcggttaag cgtcagactc gtttaattgt agtcataaat ccaaataatc ccaccggttc
     2881 agctgtctca cttga
//
LOCUS       DTVD01000043            2888 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14001_length_2888_cov_378.889869, whole genome
            shotgun sequence.
ACCESSION   DTVD01000043 DTVD01000000
VERSION     DTVD01000043.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14001_length_2888_cov_378.889869"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            68..1120
                     /locus_tag="EYO68_01550"
     CDS             68..1120
                     /locus_tag="EYO68_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC06519.1"
                     /translation="MRKLISIIISTTLTMLLVGGSAWAAKPSVGFIYVGPVGDGGWTH
                     AHDLGRKALEEAGHKTYFVESVPEGSSEGQILKIARKSDIIFTTSFGFMDPTLKVSKK
                     FPDKVFLHCSGFKQSKNMGNYFGRMYQAKYLAAMIAGGMSKKGRIGIVGSQPIPEIIR
                     HINAITLGAQAVNPNVTVEVIWINSWFDPAKESDAAQALIDNGADVIVTMADSPATVQ
                     TSKKNGVYAIGNDTDMAIYGKEAHLTAAVWNWDVYYLEAVKAVENGTWKAGSHWWGLD
                     SGIVGLTSFHPDVPQSLIIRMNKEKGKILSGAMDVFGHGFTKQDGTRVTKALNDGEML
                     GMMYYVKGIISKIPSG"
     gene            1271..1474
                     /locus_tag="EYO68_01555"
     CDS             1271..1474
                     /locus_tag="EYO68_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06520.1"
                     /translation="MKNKWSAFLDQNYDVPEVQFALKSVKILTGTKKRFLKHLSFETE
                     PAEYGKALSAYKLDIQLEEEKNG"
     gene            1467..>2888
                     /gene="gatA"
                     /locus_tag="EYO68_01560"
     CDS             1467..>2888
                     /gene="gatA"
                     /locus_tag="EYO68_01560"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIC06521.1"
                     /translation="MVDEALSRMSLTEISEALVKREVSSQEVVRNSLEELEKRGPSLN
                     CVARLFPEQALAEAESADQELSSGISPGPLHGVPLLHKDMFYRTGKISACGSKICEDY
                     APDATATALLKLDQAGALDIGRLKMVEFAYGLTGHNEITGDVRNPWNPEYIPGGSSSG
                     PAAAVAGFLSYGSLGSDTGGSIRFPASCCGLVGMKPTYGRVSRFGAMPLSFSLDHIGP
                     LTRTVADCALLTQIIAGKDTNDSTSYYQPKPDFLSGIESGIQGLKIGVPTTYGNGPSG
                     FLDQLHPEVQREMESSLAVFRSLGAKVVEIPLPENFEISNNMANIIAGAESSSAHANW
                     LKEQPEDYGSQTRERLLTGLLFSAVDYLDALKLRKVILEDFLKQVFDKVDVLHAPVVP
                     IPVPTLEESNIQANPGFIEYLTLLGHFTRPFDYLGLPALSVPAGLTDNGLPSGFQLVA
                     PPFEEALLFRTARAFEKENPWSYP"
BASE COUNT          788 a          599 c          691 g          810 t
ORIGIN      
        1 ccggctttgt gccggattct gaaacgaacc gatttgaggt tcttttaacc tttctctagg
       61 agataaaatg agaaaattaa tttcaataat aatatcaacc actctaacca tgttattggt
      121 aggagggtct gcttgggctg caaaaccaag tgttggtttc atttatgttg gccccgttgg
      181 agacggaggt tggacccatg ctcatgactt gggacgcaaa gcattagagg aagccggaca
      241 taagacatat tttgtagaga gtgttcctga aggatcttct gagggacaga tcttaaagat
      301 tgcacgaaag tccgacataa tttttaccac cagttttggt ttcatggatc caactttgaa
      361 agtctcaaaa aagtttcctg acaaggtgtt cttgcactgc tccggattta agcaaagcaa
      421 aaacatgggt aactattttg ggcgaatgta ccaggccaag tatcttgctg caatgattgc
      481 aggaggaatg agtaaaaaag gacgtatcgg aattgtcggc tcgcagccta ttccggaaat
      541 tataaggcat atcaatgcta tcaccttagg tgcacaggca gtgaatccta atgttacggt
      601 agaggtcatt tggataaaca gttggtttga tcctgccaaa gaaagtgatg ccgcacaagc
      661 actcattgat aatggtgcgg acgtgattgt aaccatggca gactctccag caacagtgca
      721 aacatcaaag aaaaatggtg tttatgctat tggaaacgat acggacatgg ctatctatgg
      781 taaagaggct catttgaccg cggctgtctg gaactgggat gtctattatc ttgaagccgt
      841 caaagctgtg gaaaacggaa cctggaaagc aggctctcat tggtggggac ttgacagtgg
      901 aattgtcggc ctgaccagtt ttcatccgga tgttcctcaa tctttaatta ttcgaatgaa
      961 taaggaaaag gggaaaattc ttagcggagc aatggatgtt ttcggccatg gatttaccaa
     1021 acaggatgga actcgtgtta caaaagcctt aaatgacggc gagatgctcg gcatgatgta
     1081 ctacgtcaag ggaattattt ccaagatacc ctccggttaa ttgttttctt tttaaattca
     1141 gacaaacaga tgtcgggtta ggctttgact taacccgaca actggcataa tcgattaaaa
     1201 gagtcttttt acctgactct aggcttttcg gcaagaaaag ttgaactatt ccctttacta
     1261 aaaaattccc atgaaaaata aatggtctgc ttttcttgat cagaactacg acgttccgga
     1321 agttcaattt gctttgaagt cagttaaaat cctcaccggg actaagaaaa ggtttttgaa
     1381 acacctttca tttgaaactg aacctgcaga atatggaaaa gctctttccg cttataaatt
     1441 agacattcaa ctagaagagg agaagaatgg ttgatgaggc tttgtcccgc atgagcctga
     1501 ctgagatctc cgaagcactt gtcaagcggg aggtatcttc acaggaagtt gtcaggaaca
     1561 gtctggaaga actagaaaaa cgaggcccct cacttaattg cgtggctcgt ttgtttccgg
     1621 aacaggcact ggccgaagct gaatcagccg atcaggagtt atcctccgga atctcgccgg
     1681 gaccactgca cggcgtaccg cttttgcata aagacatgtt ttaccgtaca ggtaaaatct
     1741 ctgcgtgtgg ttctaaaatt tgtgaggatt atgctccgga tgcgactgct acagctctgc
     1801 tcaaactcga ccaggctggc gcattggata tcggcagatt gaaaatggtc gagtttgctt
     1861 acggactgac cggtcacaat gaaataactg gtgatgtccg caatccttgg aatccggaat
     1921 acattccggg tggttcttca agcggtccgg cagcagcagt agctggtttt ttgagttatg
     1981 gctcacttgg ttctgacaca ggagggtcaa ttcgctttcc tgcatcttgc tgcgggctgg
     2041 ttggcatgaa accaacttac ggacgtgtca gccgttttgg tgcaatgccg ctctcatttt
     2101 cactggatca tatcggtccg cttaccagaa ctgttgcaga ctgtgctttg ctcacacaaa
     2161 ttattgcagg taaagataca aacgattcca cttcttacta tcaaccaaaa ccagattttc
     2221 tttccgggat agagtcagga attcagggtt tgaaaatagg tgttccgaca acatacggca
     2281 acggaccttc aggtttttta gatcaactgc atccggaggt acagagggaa atggagagta
     2341 gcttagctgt ttttcgcagc cttggtgcaa aagttgttga gataccatta ccggagaatt
     2401 ttgaaatcag caacaacatg gccaacataa ttgccggtgc agagagcagt tccgcccatg
     2461 caaactggtt gaaggaacaa ccggaagatt acggttcgca gacgcgtgaa cgtctgctga
     2521 ccggtttgtt gttttctgca gttgattacc tagacgcact taaactgcgc aaggtcattt
     2581 tggaggattt tctaaagcag gtttttgaca aagtggatgt gcttcacgcg cctgttgtgc
     2641 caattcctgt tcctacactg gaagagtcaa acatacaggc aaatccgggt ttcatcgaat
     2701 atcttacttt gctcggccac tttacacgcc cttttgatta tcttggcctt cctgctttgt
     2761 cagtaccggc cggattgaca gataatggtt taccttccgg ttttcagtta gtagctccgc
     2821 cttttgaaga agctctgttg tttcgtactg ctcgtgcttt tgaaaaagaa aatccgtggt
     2881 cctatcct
//
LOCUS       DTVD01000044           11182 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1417_length_11182_cov_330.103981, whole genome
            shotgun sequence.
ACCESSION   DTVD01000044 DTVD01000000
VERSION     DTVD01000044.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_11182_cov_330.103981"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="EYO68_01565"
     CDS             <1..786
                     /locus_tag="EYO68_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06522.1"
                     /translation="TIRFYQDYRLSNYDWNYQSYIERWSQLVYYHWRNHPPSDYLSGS
                     KPEGGEVFVQATVERSGIVSSYQVNSRGEISDIMREAAKEATLLVPLPPLPEAFPDEE
                     LKVEFRFDHPPMHHLAESNTNQVKEVSLLQDNNSVEGNDSLSKMAKKLIKKHRITSKM
                     AKKLIKKLRLELSRSSFHEELRQEFSSHFRPHQRFDPSLELKIELSIDRFGKVVEQKL
                     TQPGKSVKFQLAVLNGLSQARFGDLPKSLRSEAPYSVRLRVIP"
     gene            complement(808..1509)
                     /locus_tag="EYO68_01570"
     CDS             complement(808..1509)
                     /locus_tag="EYO68_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIC06523.1"
                     /translation="MTLVFVSLLFAVCSGILASKKNRNFIGWFFTGLLFGPFALFVVL
                     LPLLPEKTKDNSKLWTSWKFWTVWVSLFGLIALLAFGFTTDPKNVPSPLVGRPAPDFE
                     LTALKGEETIHLSELRGTPVLLNFWASWCQECKVEAHILEEFHKKYGSETEQIRVIGI
                     AIQDTLQNAQAFARRFGKSYYLGLDDDAGNIALDYGIYGVPESFFIDPDGMIFYKQIG
                     GVTPELLANKFEPFL"
     gene            complement(1648..2295)
                     /locus_tag="EYO68_01575"
     CDS             complement(1648..2295)
                     /locus_tag="EYO68_01575"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIC06524.1"
                     /translation="MNSGKLITFEGLDGCGKSTQLEKVHDWLTSRGYTVLKTREPGGT
                     KIGRQIRSILLNPEHKELQPESELLLYLSDRIQHLQETIIPAKKAGKIVLCDRFHDST
                     VAYQGYGRGLNLSSIESIVEHCINPYAPDLTIFLNISPETVAVRLEQRQKQTKKNRLD
                     LESLNFFKRTAQGFKALSAAEPERYVCLDGEQKIEVIHHEIVAVLLKRFHFQDLS"
     gene            complement(2329..2730)
                     /locus_tag="EYO68_01580"
     CDS             complement(2329..2730)
                     /locus_tag="EYO68_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06525.1"
                     /translation="MAETKETGEKAVKFGKTQDVSLKTIEKAPKDTQQKYHAISSKGE
                     SLKIVEADVLSSSTKDDIKTQLPKAIVVKKNLKKDVEILYASFKKFKETHSNAEEIKE
                     FKMACDKVILAAQKSHTEIKEKVYTIYDKKK"
     gene            2906..3223
                     /locus_tag="EYO68_01585"
     CDS             2906..3223
                     /locus_tag="EYO68_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HIC06526.1"
                     /translation="MLKIKKKAKPEKILLGDEVYILWQDGEESHISFFDLRDACPCAS
                     CIDELSGKKTLDTGSIHKDIHSLRSEYVGNYALRIYWSDSHDTGIFHFKMLRDFAKSR
                     DFT"
     gene            3261..3731
                     /locus_tag="EYO68_01590"
     CDS             3261..3731
                     /locus_tag="EYO68_01590"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIC06527.1"
                     /translation="MVQIEGDLQAANLKIAFVVARFNEFICEHLLEGALDSFVRHGGN
                     KDNVTVIRVPGAFELPLAAKRIAMNGKNDAVVCLGAVIRGETSHYDYVCANASSGIAS
                     ASLETGVPVSFGLLTTDTIEQAIERSGAKAGNQGRDATDCAIEMARLLKQISEA"
     gene            3836..4024
                     /locus_tag="EYO68_01595"
     CDS             3836..4024
                     /locus_tag="EYO68_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06528.1"
                     /translation="MNSSLKHIVLQLEDLTQQDISIGLGLDLLEASAKTRKDVIMINV
                     MRDSFTEMLVEERQCQSF"
     gene            4187..4876
                     /locus_tag="EYO68_01600"
     CDS             4187..4876
                     /locus_tag="EYO68_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC06529.1"
                     /translation="MFDVIAFDADDTLWHNESIFTMTQQKFREMLSSHGPEVVDQTLS
                     STQLKNLKHFGYGIKGFILSMIETSIELTGGSVSGKEIQQVLDFGREMLAAPIELLPH
                     VHEVVEELSKNFHLLLITKGDLIDQEIKIARSGLADYFAAVEIVSDKTAETYKKILTQ
                     HKINVSRFMMIGNSMRSDIVPIIQIGGHAVHIPYSSTWEHEQDHPSVDINHYSELKHM
                     GMLPKLIKEKK"
     gene            4873..6003
                     /locus_tag="EYO68_01605"
     CDS             4873..6003
                     /locus_tag="EYO68_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIC06530.1"
                     /translation="MTVSLLEGRKVALVHDWLNGMRGGEKCLEVLCELFPEADLFTLI
                     HDKGKLSRTIESMRIRTSFIQNMPFGLKKYRHYLPLFPLAIEQFDFSGYDLIISSSHC
                     VAKGVKHDDSVYHISYIYTPMRYVWDQFETYFRQPRTSWPVRIGAELMRPYLRHWDRN
                     SSKRVDTFLCISHNIRRKILDYYSRESQVIYPPVDLSRFRPGDTKKSYYLMVGAFAPN
                     KRVDLAVEAFNRLKLPLKIVGSGQDEEYCRSIAGENIEFLGDLSSEKLVELYQQARAF
                     LFPGEDDFGITPLEAQACNTPVIAFASGGALETVTEQTGLFFNDQKVDSLCEAVVEME
                     RIWMNFTPEAFQQQTERFGRKIYKNQMAHAIEYGYKQWKDGI"
     gene            5985..6761
                     /locus_tag="EYO68_01610"
     CDS             5985..6761
                     /locus_tag="EYO68_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06531.1"
                     /translation="MEGRNLKKYIKLLLIIQIYMIMLFSAGFLRIPPAHAFFEEQVKY
                     GFEHRWLPMLVDTAPEKRFQAMQAFLTYPEWGLPVLRNSIKTPESDNHSWQIAMLIGM
                     LGDASDIPLLLTSWRQLDKHERSEVWLGAMQRLYWKNYVPSEIIPKLKSLSVKYSKNV
                     AEGDKDSNKSDLLYEIVNPAPVSRLIRVTLQFWQTRIQEKLPAKYFLIPAGGLLKTTM
                     PVRILPVAHAKSVRLDFRIWEVGVSEPLLHRTVSIPSTGF"
     gene            6890..7345
                     /locus_tag="EYO68_01615"
     CDS             6890..7345
                     /locus_tag="EYO68_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIC06532.1"
                     /translation="MLKLSKKTDYAIILLTHLGEEEVPVSAQEVATHYKLPYPMVANI
                     LKQLVSSGLIESTRGKHGGYVLAKPADEINLAEIIMVTDSPFNLVECVHDEDNCKVHQ
                     CCPTRGPLLALHHKIQQFVEETTLAAIIRDAKPINLTVEESYYETAHLS"
     gene            7323..8537
                     /locus_tag="EYO68_01620"
     CDS             7323..8537
                     /locus_tag="EYO68_01620"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIC06533.1"
                     /translation="MKLPIYLDYNATTPVDPKVVETMMPFFTESFGNAASRNHSFGWQ
                     VEKVVEDARESIGNAIGAGGKEIVFTSGATESDNLAILGVANFYQKKGKHIITSPIEH
                     KAVIDPCHALEQQGYEITFLNVEKNGCIDLNQLADSIRDDTILVSLMAGNNEIGTLNP
                     LKEIGEITREKGVLFHTDATQGVGKIPIDVEEMKIDLLSMSAHKMYGPKGIGALYVRR
                     KKPRVRLSPIMFGGGHERGMRSGTLNVTGIVGFAKALELSMDEMASEAERLTGLRQRL
                     FERLTSELDYIFLNGHPTERLPNNLNLSFGYVEGESLMMGVSELAVSSGSACTSASLE
                     PSYVLRALGVGDDLAHSSIRFGMGRFTTVEEVDYAAEQMISAVKRLREMSPLYEMVQE
                     GVDLSTVKWSSH"
     gene            8574..8960
                     /gene="iscU"
                     /locus_tag="EYO68_01625"
     CDS             8574..8960
                     /gene="iscU"
                     /locus_tag="EYO68_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIC06534.1"
                     /translation="MAYSEKVIDHYKEPRNVGSLDKNNTNVGTGLVGAPECGDVMKLQ
                     IQVNPETNEIVDAKFKTFGCGSAIASSSLATEWVKGKSIDQAMSIQNTQIVEELSLPP
                     VKIHCSVLAEDAIKAAISDYKSRRED"
     gene            8991..9608
                     /gene="hscB"
                     /locus_tag="EYO68_01630"
     CDS             8991..9608
                     /gene="hscB"
                     /locus_tag="EYO68_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIC06535.1"
                     /translation="MTFEQPACQKCESPLISRLFCLSCNAFQTVPPEIDYFEVLGFPV
                     CFEINSEDLEERYKRLSLVLHPDFFAAAPEEEKRLSEKASAMLNTAYNSLRETTSRAG
                     YLLSLFTKEKNLNERTLPDGFLQEMFFLQESLDELLESSDSSALNKMNEDLRTRHKEI
                     ESYYAPLFKNFKDLPEDSDILQQLQTHLNAERYLRRLLDRIPATD"
     gene            9771..>11182
                     /locus_tag="EYO68_01635"
     CDS             9771..>11182
                     /locus_tag="EYO68_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscA"
                     /protein_id="HIC06536.1"
                     /translation="MKRIIGIDLGTTNSLSATVFDEGPEVIGEGENAVTPSVLSRSNS
                     GWLVGQEAQERRITYPENTVYSIKRLMGRSLDELSETVQDLPYQIVEAQRQLVKVRIG
                     ESKPYKEFTPQELSAEILKKVKQRAEEALGESVEKAVITVPAYFDDAQRQATRDAGRI
                     AGLEVARIINEPTAAAIAYGLDEKRDLQKDGHVAVYDLGGGTFDISILKLSGKIFKVV
                     ATHGNTQLGGDDFDQAVVETLKDRIHQEHPEAEFDDPESKQILKKTAEFIKIKLSLSP
                     ETDYSLDFSKKSMSFSGVFKLEEFESLIAPMIEQTLESCRHVLREAKLSVKKIDEVVL
                     VGGSTVIPAVRKQIESFFGRPPHVAIDPYKVVAIGAGIQGHLLAGGRRDFLLLDVIPL
                     ALGIETLGGTFSKIITGNTTIPIEESETFTTNVDNQTSIDVNIFQGERELIKDCRALG
                     QFKLRGIPPMKAGLPLVEVM"
BASE COUNT         3336 a         2138 c         2417 g         3291 t
ORIGIN      
        1 acaattcgtt tttatcagga ttaccggctc agcaattacg attggaatta tcaaagttac
       61 attgaacgtt ggagccaatt agtatattac cattggcgca atcacccacc gtcagattat
      121 ttatcaggtt ccaaacctga aggcggagag gttttcgtcc aggctaccgt tgaaagaagt
      181 gggattgtga gcagttatca ggtcaatagc aggggtgaaa tttcagacat catgcgtgaa
      241 gcagcaaaag aagcaaccct cctggtccca ctgcctcctc ttccagaggc gtttcctgat
      301 gaagagttaa aagttgagtt ccgctttgac caccctccaa tgcatcatct ggctgaatca
      361 aatacaaatc aggtgaaaga agtttcgctt cttcaagata acaattcagt agaaggaaac
      421 gatagtcttt caaaaatggc aaagaaactc ataaaaaaac atcgtatcac atcaaaaatg
      481 gcaaagaaac ttataaaaaa acttcgtctc gaattgagca ggtcatcttt tcacgaagaa
      541 ttacggcagg aattttcctc acattttcga ccgcatcaac gatttgatcc tagtttggaa
      601 ctgaaaatag agttaagtat agaccgtttc ggtaaagtgg tggaacaaaa actgacccag
      661 ccgggaaaat cagtaaaatt tcagcttgcc gtgctgaatg gactgagtca agcacgtttt
      721 ggcgacttgc ctaaatcctt acgttctgaa gctccctata gtgtgcggtt aagagttata
      781 ccctgaaaac atgatttgca gcctctttca cagaaatggc tcaaatttat ttgccagtaa
      841 ttctggagtc actcccccaa tttgtttata gaaaatcata ccatccggat cgatgaaaaa
      901 agattccggc actccataaa ttccataatc caaggcaata ttaccggcat catcatccaa
      961 gcccagataa tagcttttac caaaacggcg tgcaaatgct tgtgcgtttt gcagagtatc
     1021 ctgaatggca atacctatta ctcgaatttg ttctgtctcc gaaccatatt ttttgtgaaa
     1081 ttcttccaga atatgggctt ccaccttaca ttcttgacac catgaagccc aaaaattcag
     1141 taacacaggc gtacctctta gttcacttaa atggattgtt tcctcgcctt tcaaagccgt
     1201 caactcaaaa tctggagcag gtcgcccaac cagaggagaa ggcacatttt tggggtcggt
     1261 agtaaaccca aaggccaaca gagcaatcaa tccaaataaa gaaacccaaa ctgtccagaa
     1321 tttccaagat gtccagagct tagaattatc tttggttttt tccgggagta aaggaagtaa
     1381 tactacaaat aaagcaaagg gaccaaataa tagtcctgta aagaaccagc caataaagtt
     1441 tctatttttt tttgatgcaa ggataccact acaaacagca aataacagtg acacaaaaac
     1501 aagcgtcata actatcgaac ctgagaattc catgtaattt tattaggagt tatttaaaat
     1561 ggatagagtt aaaacactgg gacgtttgtt cctttaaaca actttccctt accttaggtc
     1621 cctaaacgaa attagcagcc aaaattccta actgagatcc tggaaatgga atcgtttcag
     1681 taagacagca acgatttcat gatggataac ttcaattttc tgttcgccgt cgaggcaaac
     1741 atagcgttca ggttcagctg cagataaagc tttaaatcct tgtgcagtac gtttgaaaaa
     1801 gttcaatgat tccaggtcaa gtcgattctt ttttgtttgt ttttgacgct gctccaaacg
     1861 tacagcaacg gtctcaggag aaatattcag gaaaatagtc aaatccggag cataaggatt
     1921 tatgcaatgc tctacaatag actcaatgct gctcaaattc aagcctctgc catagccctg
     1981 ataggctact gttgaatcat gaaagcggtc acacaggaca atctttccag ccttttttgc
     2041 cggaattatt gtttcttgaa ggtgctggat ccgatctgac aaatagagca gcaattcact
     2101 ttcaggctgt agttctttat gttccggatt gagaagaata ctacggattt gccggccaat
     2161 ttttgtgccg ccgggctctc tggttttgag gacagtataa ccacgactgg tcagccagtc
     2221 atgtactttt tctaactggg tactctttcc gcagccatca agtccctcaa aagtgataag
     2281 ttttccggaa ttcatggatt tggggtatca aaatagaaag gaagggattt atttcttttt
     2341 atcgtaaatt gtgtagactt tttcttttat ttcagtatga gatttttgtg cggccaggat
     2401 caccttgtcg catgccatct tgaattcttt tatttcttca gcattactgt gagtttcctt
     2461 aaattttttg aagctggcat acagaatttc tacgtctttt ttgagatttt tctttaccac
     2521 aattgcttta ggcaattgtg tttttatatc atctttggta ctgctgctga gaacatcggc
     2581 ttccactatt ttcagggatt ctccttttga tgagatagca tgatattttt gctgcgtatc
     2641 ctttggagcc ttttcaatag tctttagtga tacatcctgt gttttgccga attttacagc
     2701 tttttctcct gtttctttgg tttcagccat ggtttttctc aaattgttat gtaaaaatta
     2761 caatgttcct cacgatagca tacattgtta tgcctaagga atgcttgagt tgttctttca
     2821 attactgtaa attaacatgc aattctattt ttctaactct accaccttgt aaaaagagta
     2881 acttagttga aacgttctgc ttattatgct taaaataaag aaaaaagcca aaccggaaaa
     2941 aattctttta ggagatgaag tatatatcct ctggcaagac ggtgaagaaa gccatatcag
     3001 tttttttgat ttgcgggacg catgtccctg tgcaagttgt attgacgaac tcagcggtaa
     3061 gaagactttg gacaccggaa gtatccacaa agatatacac tccctgagaa gtgagtacgt
     3121 gggtaactat gctcttcgta tttactggag cgatagtcat gacacaggta ttttccattt
     3181 caagatgctc cgtgattttg cgaaatctag agattttaca taagactgat taagcttaaa
     3241 tttataattt aaggagaaac atggttcaaa ttgaaggtga cttacaggcc gctaatctga
     3301 aaatagcatt tgtggtggca agatttaatg aatttatctg tgaacatttg ctggaaggtg
     3361 ccctggactc ttttgtgcgt cacggtggaa ataaagataa tgttaccgtg atacgtgtcc
     3421 caggagcatt tgaacttccg ttagcagcaa aaagaatagc catgaatgga aaaaatgatg
     3481 ctgttgtttg tttgggcgct gtaattcgtg gtgaaacatc gcattatgat tacgtttgtg
     3541 caaatgcgtc cagtggaatt gcttcagcct cattggaaac cggagtaccg gttagttttg
     3601 gtctcctgac gacggatacc attgaacagg ccatagagcg ttccggtgct aaagctggta
     3661 accagggtcg tgatgcaacg gattgcgcaa ttgaaatggc acgtctttta aaacaaatat
     3721 cggaggcata gtaactgcat cgaataagcg gtataccgga agatagttgt ttaggcacgg
     3781 gtttttttga aatctggcca atgattccgg ttaatttgaa gccgtaagga ggaagatgaa
     3841 ttcttcgcta aaacatattg ttttgcagtt ggaagactta actcagcagg acatttcaat
     3901 tggtctggga ttggatctat tggaggcatc ggcaaagaca cgcaaggacg ttataatgat
     3961 caacgtaatg cgtgattcct ttacagaaat gttagttgag gagcgccagt gccaatcttt
     4021 ttgaagcctc ttgggtgaga ttgtttatct cacccggagc tgtcttcaaa aacagctgaa
     4081 gctatgtttt tccttcctga tttttacggt tacttctgaa ttggcttcct gccaactgtt
     4141 tatctcttct gcttttaccc gctctctgat tacaacaaac taaacgatgt ttgatgtaat
     4201 tgcctttgat gccgatgata ccctctggca taatgagtct atctttacta tgactcagca
     4261 aaagtttcgt gaaatgcttt cgtcccatgg acctgaagta gtagatcaaa ctttatcttc
     4321 aactcagtta aagaatctca agcacttcgg ttatggaatc aaaggtttta ttctttctat
     4381 gattgagaca tcgattgaac tgacaggtgg cagtgtaagc ggtaaagaga tacagcaggt
     4441 tttggatttt ggacgcgaaa tgttagctgc ccccattgaa ttgctgccgc atgttcatga
     4501 agtagttgaa gaattatcca aaaattttca cttgctgctt atcaccaagg gcgatttgat
     4561 tgatcaggaa ataaaaattg cacgctccgg attggctgac tattttgctg ctgtggaaat
     4621 tgtcagtgat aaaaccgcag agacgtataa aaaaatatta actcaacaca aaattaatgt
     4681 atcgcgtttc atgatgattg gaaattcaat gcgttcagac attgtaccaa ttatacaaat
     4741 tggagggcac gcagtacaca ttccttactc ttctacctgg gagcatgagc aggatcatcc
     4801 atccgttgat ataaatcact attcggaatt gaaacacatg ggtatgcttc caaaactgat
     4861 caaagaaaaa aaatgacagt atcgctactg gaggggcgta aagtggcgct ggtacacgat
     4921 tggctgaatg gaatgcgtgg cggtgaaaaa tgtttggagg tactatgtga actgtttcct
     4981 gaagcagatc tttttacttt aattcatgat aagggcaagc tttcacggac gattgagtcc
     5041 atgcgcatcc gtacatcttt cattcagaat atgccctttg gtttaaaaaa ataccgacat
     5101 tatcttcctc ttttcccact ggcgatagag cagtttgatt tcagcggata tgatcttata
     5161 atcagttcca gccattgtgt tgcaaaagga gtgaaacacg atgactcggt ctatcatatt
     5221 tcatacattt acactccaat gcgttatgta tgggatcagt ttgaaactta ttttcgtcag
     5281 cctcgaactt cctggcctgt cagaattggt gcagaattaa tgcgtcccta tttacgacat
     5341 tgggatcgaa attcgtcgaa acgtgtagat acttttcttt gcatcagcca caacattcgc
     5401 cgaaaaattc ttgattatta cagccgtgag tcacaagtta tttacccacc agttgatctt
     5461 tcccggttta gaccaggaga cacaaaaaaa agttactatc taatggtagg agcttttgca
     5521 ccaaataaaa gggtggattt ggcagtggaa gcttttaacc gactaaagtt accgcttaaa
     5581 atagtgggta gcggtcaaga cgaagaatat tgcaggtcaa ttgctggaga aaacattgaa
     5641 tttcttggtg atctttcgag tgaaaaactg gtagaattat accagcaggc gcgtgccttc
     5701 ttgtttccgg gcgaagacga ttttggcatt accccgctgg aagcacaggc atgcaatact
     5761 ccggtgattg cttttgcttc cggaggtgct ttggaaacgg taactgagca aacaggattg
     5821 ttttttaatg atcaaaaagt tgactcactt tgcgaagcag tagtcgaaat ggaacgaatc
     5881 tggatgaatt ttactccgga agcatttcag cagcaaacgg aacgctttgg caggaaaatt
     5941 tacaaaaatc agatggctca tgccattgag tacggataca aacaatggaa ggacggaatt
     6001 tgaaaaagta tataaaatta cttctgataa tccagatcta catgattatg ttattttccg
     6061 ctggattctt aagaataccg ccggctcatg ctttttttga agaacaggtt aagtatggct
     6121 ttgagcaccg ctggttaccg atgttggttg atactgctcc agaaaaacga tttcaagcaa
     6181 tgcaggcatt tttaacatat ccggaatggg gtctgcctgt tctaagaaac tcaattaaga
     6241 ctccggaatc tgataatcat tcctggcaaa ttgcgatgtt gatcggtatg cttggtgacg
     6301 cgtcagatat accgcttttg ttaacaagtt ggcgtcaact ggataaacat gaacgctcag
     6361 aagtttggct gggagcaatg cagcgtttat actggaaaaa ttatgtgcct agcgagatta
     6421 ttccaaaact aaaaagtctt tcagtaaaat attcgaaaaa tgtagctgaa ggagataaag
     6481 acagcaataa gtcagactta ctttatgaga tcgtcaatcc tgctccagtg tcccgcctca
     6541 ttcgagtgac cttacaattt tggcaaactc gaattcagga aaagcttcct gccaaatatt
     6601 ttttgattcc agccggcggc ttgctcaaaa caaccatgcc ggttcgtatt ttgccggttg
     6661 cacacgccaa aagtgttcgt ttagatttcc gcatctggga agttggtgtt tcagaaccgt
     6721 tgcttcatcg gacagtttca attccttcaa caggttttta acccattcaa ttataataat
     6781 ttcttcttgg ctgaattttt ataagtaaaa aaaatcaatt ttttttatta tttgcttgat
     6841 atagtactaa attagtgcta ttttaagaaa actaataaaa aggagggcga tgctgaaatt
     6901 atcaaaaaag actgattacg caattatttt gctgactcat ttgggtgaag aagaagttcc
     6961 tgttagtgcg caggaagtgg cgacgcatta caaattgccc tatcctatgg tggccaatat
     7021 acttaaacaa ctagtgtctt ccggattgat tgagtcaact cgtggaaaac acgggggata
     7081 tgtcctggct aagccggcag atgaaatcaa cttggcagag attatcatgg tcactgatag
     7141 tcccttcaat ttagtagaat gtgtccatga tgaagataat tgcaaagtac atcagtgctg
     7201 tcctacacga ggacccctgc ttgctttaca tcataaaatt caacaatttg tcgaggaaac
     7261 aacattagcc gcgataatca gagacgcaaa accaattaac ttaactgtag aggaatctta
     7321 ttatgaaact gcccatttat cttgattata atgccaccac tccggttgat ccaaaagtgg
     7381 ttgagacgat gatgccgttt tttacggaat catttgggaa tgccgccagt cgtaatcatt
     7441 catttggttg gcaagttgaa aaagtagtgg aggacgcccg agaatcgatt ggcaacgcca
     7501 ttggtgcagg cggcaaggaa atcgttttta ccagcggagc cactgaatca gacaatctcg
     7561 ctatcttagg agtagcaaac ttttatcaaa aaaagggcaa acatatcata acaagtccga
     7621 ttgaacataa ggctgttatt gatccttgcc acgcattgga gcaacagggt tacgaaataa
     7681 cctttctcaa tgtggaaaaa aatggctgta tcgatctaaa tcaattggca gattctattc
     7741 gtgacgatac gattttggtt tcactaatgg ccggcaacaa tgaaatcggc acattgaatc
     7801 ctctcaaaga aattggcgaa atcactcgtg aaaagggagt gctttttcat acagatgcaa
     7861 cgcagggtgt gggtaaaata ccgattgatg tagaggagat gaagatagac cttctttcaa
     7921 tgtctgcaca taagatgtat ggtccaaaag gtattggcgc attgtatgtc cggcgtaaaa
     7981 aaccacgtgt ccgtttgtca ccaatcatgt ttggtggagg tcatgaacga ggtatgcgtt
     8041 ccggaactct gaatgtaaca ggaattgtag gattcgccaa agctcttgaa ctatcaatgg
     8101 atgaaatggc ttcagaagca gaacgattga caggattgcg ccaaaggctt ttcgaacgtt
     8161 taacctctga attggattat atatttctca atggtcatcc gactgaacgt ctgccgaata
     8221 atctcaactt gagtttcggt tatgtggaag gtgagagcct gatgatgggc gtgagtgaac
     8281 ttgcggtttc ttccggatca gcctgcactt cagcaagttt ggaaccgtct tacgttttac
     8341 gggcattggg agttggagac gatctggcgc attcgtccat tcgtttcggc atgggacgtt
     8401 ttactactgt tgaagaggtt gattatgctg cggagcaaat gatatctgca gttaaacgcc
     8461 tccgtgaaat gtcaccgctt tatgaaatgg tgcaggaggg tgttgatttg tcaactgtta
     8521 aatggtcatc tcattgaact taaactacat acatatttga aaaaaggagg aacatggctt
     8581 atagcgaaaa agttattgat cattacaaag agcccaggaa cgtcgggagt ttggataaaa
     8641 ataataccaa tgtcggtacg ggattggttg gtgctccgga atgcggtgat gtaatgaaac
     8701 tgcaaattca agtgaatcct gaaactaatg aaattgtaga tgctaaattc aagacttttg
     8761 gctgtggatc tgccattgct agttccagtc tggcaacaga atgggtaaaa ggcaaaagta
     8821 ttgatcaggc aatgtctatt cagaatacac aaattgttga agaactttcg ttgcctccgg
     8881 taaaaattca ttgctctgtt ttagccgaag acgccattaa agccgcaatt tctgattata
     8941 aaagtagaag agaagattga gttatagcga ttatttttta aaaacgagct atgacttttg
     9001 agcaaccggc ctgtcaaaaa tgcgaatctc cgcttatttc gcgccttttt tgcctgtcct
     9061 gcaacgcctt ccaaactgtt ccacctgaaa ttgattattt tgaagtgctg gggtttcctg
     9121 tttgctttga aattaattct gaggatttgg aagaacgcta caagcgttta tcgttggtct
     9181 tgcatccaga ctttttcgcg gcagcaccag aggaagaaaa acgtttgagc gaaaaggctt
     9241 cagcaatgct gaacacagcc tacaattctt tgcgtgaaac aacttcccgt gccggatatt
     9301 tattatcttt gtttaccaaa gaaaaaaacc tgaacgaacg tacattacca gatgggtttt
     9361 tgcaggaaat gttttttttg caggaatcac ttgatgaact gcttgaatca agtgattcgt
     9421 ctgcccttaa taaaatgaac gaagatttac gaactaggca caaagaaatt gaatcttact
     9481 atgctccatt gtttaaaaac tttaaagact taccggagga ctccgatatt ctccaacaat
     9541 tacagaccca cttgaacgca gaacgctatt tgcgccgctt gttagaccgt attccggcaa
     9601 ctgattagcc gctaagtgca ctaagaatcg ctaagttttg gatttatatt ttactgatta
     9661 aaatgattat ttaccctgca gatctgtgtt caacagtgtc ctgtgaatca agattattag
     9721 tgtttcttag cgtacttagc ggctaaaaac aaaccacaaa tgaacaataa atgaagcgca
     9781 taatcggcat tgatttaggc acaacaaaca gtttgtccgc aactgttttt gatgaaggac
     9841 cggaagtgat tggcgagggt gaaaacgctg ttacaccttc cgttttgtcg cgcagcaatt
     9901 ccggatggtt ggttggtcag gaagcacagg agcgaagaat aacatatcct gaaaatacgg
     9961 tttattcaat caaacgtttg atgggacgga gtttggatga gttgagcgaa acggttcaag
    10021 atctgccgta ccaaattgtt gaagcccaaa ggcagttggt caaagtcagg attggtgaaa
    10081 gcaaacctta caaagaattt acgccacagg aattatctgc ggaaattctg aaaaaagtaa
    10141 aacaacgtgc cgaagaagcc ctgggtgaat ctgttgaaaa agctgtaatc actgttcctg
    10201 catattttga tgatgcacaa aggcaggcaa cacgtgatgc cggcagaatt gccggactgg
    10261 aagtcgcacg tatcatcaat gaaccaacgg ccgcggcaat tgcttatgga cttgatgaaa
    10321 agcgagactt acaaaaagac ggacatgttg ccgtttatga tttaggagga ggtacttttg
    10381 atatttcaat tctcaagctc tctggaaaga tttttaaagt tgtggccaca cacggaaaca
    10441 cccaacttgg cggagatgat tttgatcagg cggtagtgga aacattgaaa gaccggattc
    10501 atcaggaaca tccggaggca gagtttgatg atccggagtc caaacaaata ttaaaaaaaa
    10561 ccgccgagtt catcaaaata aaacttagtc tttcacctga aacggattac tcactggact
    10621 tttcaaaaaa gagtatgtca ttttctggtg tttttaaatt agaagaattt gagtctttga
    10681 tagcaccgat gatagagcag acgcttgaaa gttgtcgtca tgttttgcgt gaagcaaaac
    10741 tttcagtgaa aaaaattgac gaggtcgtac tggttggagg ttcaactgtt ataccggctg
    10801 tgcgtaaaca gatagaatca ttttttggac gcccaccgca cgtggcgatt gatccctaca
    10861 aagttgtggc aatcggtgct ggaattcagg gacatttact ggcgggcggt cgtcgcgatt
    10921 ttttgctgct cgatgtcatt cctctggctt tgggcattga aactctgggt ggaacattca
    10981 gtaaaatcat cactggaaac accactattc cgattgaaga aagcgaaact tttactacaa
    11041 atgttgataa tcagacttcg atcgacgtta atatatttca gggagaacgt gaattaatta
    11101 aagattgtcg tgcactaggt cagtttaaat tacgcggtat tccaccaatg aaagctggat
    11161 taccgctggt agaagtcatg tt
//
LOCUS       DTVD01000045           11169 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1419_length_11169_cov_267.440076, whole genome
            shotgun sequence.
ACCESSION   DTVD01000045 DTVD01000000
VERSION     DTVD01000045.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1419_length_11169_cov_267.440076"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="EYO68_01640"
     CDS             <1..1221
                     /locus_tag="EYO68_01640"
                     /inference="COORDINATES: protein motif:HMM:PF11013.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HIC06537.1"
                     /translation="AVEIHVNSSGWYSHHHEGNPDYNEVVLHVVLYHNGQREIKRQDG
                     RIVPELELAPLLSKEPSTSETEAKNHLKKLSELPGRCGIAALERGTETLKKILGHAAE
                     QRIQEKTEVLLKRWDEQDPEELLFQLLFKSLGYSPYAQVFEELAKQYQFRELRPLFRQ
                     SQRTTRTLVLSRWFGACGLFSKKMTIADPTVRHEFQQWKAAWQELPEHPQVSGKISQA
                     HRPQNSPERRLLGMFHHLYRIANDGLLKRWLVVFRNLSVFSEEKELRRQALTETEQLF
                     STPDWEIWRKHLVLGKSKQINTAQLVGKDRQTVIWANAVLPFFLALARHENEPELEKL
                     LYQLFMILPAEASNSKTRFMEKRLWFPELSKSTKLEMNTFGNRQGLIQIQHDFCRNFH
                     QGCVRCELPRLLED"
     gene            1242..2525
                     /locus_tag="EYO68_01645"
     CDS             1242..2525
                     /locus_tag="EYO68_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06538.1"
                     /translation="MSIQQKLFLLLCLSTLVLISSCAAPKLSQFEVQRPSRITVPREV
                     KKVFIRADLVDETNDKLGIKSKVLQQLAQELNRFERFNVSVVNTLDEANIDSEKETIA
                     IIQGEVISGGEVDRGQFTDLATCTGGLGGRLSSAGAAAISDETITLDSWRGYVCRRGA
                     FASNVAEMALSSAFALAGLNEVIPPKNQVVRTYKYKNISLFAQANFSFTVIGLKRETL
                     AIRADSSSFGRSITEKDSYRNVKESHLISLTLGSLISYTRIPIFPIPIREVALASRSN
                     PKQIFYGQKPLPVPSIYDLPPKEKKKVIQKLVMLTLQSFIRTISPYKVMVDAEVASGG
                     KAKAGDLLKEGKSKQARKIIEAIPEGKRDSQDWYNLGLAYEASAVSPEDYEDARRFYI
                     TALENSPGTKLYAQGVGRTRLYLAEKRRLAKQIQD"
     gene            2542..3684
                     /locus_tag="EYO68_01650"
     CDS             2542..3684
                     /locus_tag="EYO68_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06539.1"
                     /translation="MNNYIFNQTLYILLIGAFLLTSCAKATLTVRSPAEINTRDIRNI
                     AVGKFEIGQISLKYKTERNGIWQTHPVLLNEAQQKSISRSVRARVINLLTATPYFKVV
                     FSDEFEKLENDTALQQLVSVRGYKTQNVDAVISGKLWLEIERTDGVDLSKEGLEYFRP
                     PRSRRSLGLNLTVDQVVWWPYKSTRGTLGLEIKMTRLVPAEVVATTFETRTYSQRIGG
                     RSTESFQQIAESLTSVLPTSKTTETESIEKSADVLPSFEQIIADLALSIASGFVRRVA
                     VTDKTVSYPIADGKFPNSKILIEAGAYDLAIEQLQQATAENPDPNDLYNLGLCFEAVG
                     DYGLAQTSYREAWSAEPENLLFAQGMGRIERLQRENPQLLRQLESR"
     gene            3719..4978
                     /locus_tag="EYO68_01655"
     CDS             3719..4978
                     /locus_tag="EYO68_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06540.1"
                     /translation="MKLISFQMIRFFNFAMREDIETTYIRTSVLLSICVFLLTGCAPK
                     VSFQIERPAAQRVENINYIEIGKFKIVSGQIKLPSSVESGSTQSFSSTDKLLKPTISK
                     FNSNKEQASQIADLLRAALVHNLSLHSPYQLINTTGKETGFSGVLPDAAEVAILQGKV
                     KYFERIIESEEALPYFTNIKNKGATLEQSLLASAVSMGAESSGAGFLIPTPYVEQLAA
                     LEVEFTLINKSDGSDVVPPQIIRTYYVRKWGGAPETSHLPRNIKTAIIEGFEQDEDNS
                     ESFLSRIDRAGLSFTNPTEYFARGFNLKQNVQVPQTSLDLKIKLGRQVAQTYVKQISP
                     FHETADLLVQDGNTVASTLIRGNAYQEAVAYLQGLEELTAKDEYNLGLAFEASGEIPQ
                     ARNHYELALKRETSNQDFKDAVKRTRN"
     gene            complement(5014..5448)
                     /locus_tag="EYO68_01660"
     CDS             complement(5014..5448)
                     /locus_tag="EYO68_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIC06541.1"
                     /translation="MEFDEQMAQWRETSGLTRKEFARKLSVSLTAVKNWETGHSTPKL
                     TKYSEIAKVLAIDVREMGLDNDLDLDRIGDRIKYARLLRGMSIEAFAYEHGFAIQTVK
                     SWESHAAEVTEASLERISRALKIPSPFFDMKNDPHQELADLK"
     assembly_gap    5656..5755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5998..6192
                     /locus_tag="EYO68_01665"
     CDS             5998..6192
                     /locus_tag="EYO68_01665"
                     /inference="COORDINATES: protein motif:HMM:PF00464.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06542.1"
                     /translation="MEHADQLEIMLMSLMRQLFQAKYVEYRVPSGSIANLYAYMATTK
                     PGDKIMSFSDAAAGHVTHHA"
     gene            6214..7056
                     /locus_tag="EYO68_01670"
     CDS             6214..7056
                     /locus_tag="EYO68_01670"
                     /inference="COORDINATES: protein motif:HMM:PF00464.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC06543.1"
                     /translation="MEIHEVPFDFAQMDVDPEALMIAAKKVRPKLIIIAGSMCLFPYS
                     LQEVRKVADEVGAWILYDAAHMGGLIAGGEFQRPLKEGADLMTGSTYKSFGGPPSGMV
                     FTNSAELAKRLDKIAFPGLTANFDLSRAAAMVIAVLDLLTHGREYAKMCIANAKALAE
                     SLHTGGCEVFHVSGKGFTNSQHVALPAATYGGGDTASKLLEKSNLFTSGIGLPLPPVP
                     GDFNAMRLGTQEITRWGMRPENMETIADFFCRLLLKQEKPEKLKSEVIEFRKAFQKLH
                     YIRD"
     gene            complement(7088..7576)
                     /gene="ybeY"
                     /locus_tag="EYO68_01675"
     CDS             complement(7088..7576)
                     /gene="ybeY"
                     /locus_tag="EYO68_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIC06544.1"
                     /translation="MIEIRCDEKWENLINKSELQKILTIFLKYLYAGERGISLYIAND
                     AVIQKLNREFRGKDCPTDILSWAYDEDDPGMFEAQMSEAELENSESTGVAGELVLSGE
                     RVSQQATENGWDFETELIRLLAHGCAHLAGWNHERSSEEESEMLELETELLKKVGLTN
                     IY"
     gene            complement(7573..8523)
                     /gene="rsmH"
                     /locus_tag="EYO68_01680"
     CDS             complement(7573..8523)
                     /gene="rsmH"
                     /locus_tag="EYO68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIC06545.1"
                     /translation="MKMQFEHISVLTQEVVDFAPESTQTILDCTLGGGGHSRCLLENF
                     PDAKLYGIDRDALAVKAAAERLKTYEKQTKLGQASFSELPEFFAECGEPVFDYILADL
                     GLSSEQLVRAERGFSFLREGPLDMRMDPDRQQITAAHFVNNSSEQELLKILRNFGEER
                     FARKIVGKILEFREKNPLKTTKELAQLVSSVIPRRLQKQGFNPATLTFQALRIAVNDE
                     LQELEVLLKQIPSRLKKDGRVALISFHSLEDRIVKHQFRKWENPCVCPTDLPYCICGE
                     KPRGRVLTRRPVNATKKEVADNPRSRSAHLRVFAFEKTVL"
     gene            complement(8520..9416)
                     /locus_tag="EYO68_01685"
     CDS             complement(8520..9416)
                     /locus_tag="EYO68_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06546.1"
                     /translation="MKLFLKIFIQILLLCSSSVTLWGQQQNIILTGSQNTGSYIFANE
                     LARLWSSSKTSRKIEFVIHPEISPESRLTQLENNRVTLAIIDAAIAHKELKKHPGLRV
                     LTVLWQNWLYVMGTVPGPLLSLESTQTLLVHDNSFYFAQVWKNLAPQTKINWFNTESI
                     PNFSEGFSEEVLAFTGPAPLKEVNDWLEQFPGIRLLSLNQQLIQALRLKFSWLITQKL
                     PANKYHYQTEELEGVAWYPVLVVRRDFSQEHAKTLLNLIFAQSKALNPNVLFQSLLRT
                     NNIAFKKIYAYHAAAKKMLRFK"
     gene            complement(9429..10247)
                     /gene="mutM"
                     /locus_tag="EYO68_01690"
     CDS             complement(9429..10247)
                     /gene="mutM"
                     /locus_tag="EYO68_01690"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIC06547.1"
                     /translation="MPELPEVETVVRELREEICGDIISSVEIFRSNPIVQGDLDTFQE
                     QLCGRKFMDVRRRAKYLIFNLEPKRYLVAHLRMTGKFIVSDPLPEPTKYNRVWFNLES
                     GRQMIFDDIRCFGTLEVYDDLADSKSLQKLGIEPLSKAMTLGYFKKNLGSSKREIKSV
                     LLDQQIIAGLGNIYVSEILFRSMIHPQRSAGSINKREWSLIIKYTKYILQEAIKNNGT
                     TISDFRRVDDKTGAFQQFLQVYDKKEQPCTECGTPIQRIVQQQRSTFFCPECQR"
     gene            10751..11017
                     /locus_tag="EYO68_01695"
     CDS             10751..11017
                     /locus_tag="EYO68_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HIC06548.1"
                     /translation="MAVGWAREGAVQEQIEATVEDAVQLARSRLPEGESFIKCEECEI
                     EIPAARRKAVPGVRLCIQCQEELEKKQTAISYYNRSGNKDSQLK"
BASE COUNT         3265 a         2292 c         2317 g         3195 t
ORIGIN      
        1 gcggtggaaa ttcatgttaa ttcttccgga tggtattcac atcatcacga aggaaatccg
       61 gattacaatg aggttgtctt gcacgtcgtc ctttaccaca atggacagag ggaaattaaa
      121 cgccaggacg ggcgaatagt tcctgagctg gaacttgccc ctttattgag taaagaacct
      181 tctacatccg aaactgaagc gaaaaatcac ttaaaaaaat taagtgaatt accggggcgt
      241 tgtggaattg ctgctttaga gcgtgggaca gaaacactca agaaaattct tggccatgct
      301 gcagaacaac gtattcaaga aaaaacagaa gtgctgctta aacgatggga tgaacaggat
      361 ccggaggaac tgctgtttca gttgcttttc aaatcacttg gatattctcc gtatgcacag
      421 gttttcgagg agttggctaa gcaatatcaa tttcgagaac tacgcccttt gtttcgtcag
      481 tctcagcgca caacgcgtac tctggtgctt tcacgctggt ttggagcctg cggtctgttt
      541 tccaaaaaaa tgaccattgc cgaccctaca gtacgtcatg aatttcagca gtggaaagct
      601 gcctggcagg aacttccaga acatccacaa gtttccggga aaatttcaca ggcacatcgc
      661 ccccaaaatt ccccagagcg acgtttattg ggcatgttcc accatttgta ccgcatagca
      721 aatgacggtt tactcaaacg ctggttggta gtgttccgaa acttgagtgt tttttctgaa
      781 gaaaaagagt tgcggcggca agctctgacg gaaacagaac agctattttc tactccggat
      841 tgggaaattt ggcggaaaca ccttgttttg ggtaaatcga aacagattaa taccgcacaa
      901 cttgttggaa aagaccgtca aactgtaatt tgggcaaatg cagttttgcc gtttttcctg
      961 gcccttgcac gtcatgagaa tgaaccggag ctggaaaaac ttctttatca attgttcatg
     1021 attttacctg cagaagcttc aaacagcaaa acacgtttca tggaaaaacg tttatggttt
     1081 ccagaattgt ctaaatccac taaactggaa atgaacacat tcgggaatcg gcagggtttg
     1141 attcaaattc aacatgattt ttgccgcaat ttccatcagg gctgtgttag atgcgagctg
     1201 cctcgactgt tagaagacta agtacagata tgaaaaaaac aatgtcaata cagcaaaaat
     1261 tatttttact attgtgcctc tctaccttgg tcctcatctc atcttgtgcc gcaccgaaac
     1321 tttctcaatt tgaagtgcaa agaccttcaa ggattacagt gccgcgggaa gtaaaaaaag
     1381 tatttattcg tgcagatttg gtggacgaaa caaatgataa acttggcatt aaatctaaag
     1441 tacttcaaca actggcccag gaactcaatc gtttcgagcg ctttaatgtc agtgtagtaa
     1501 acactttaga cgaggccaat attgattcgg aaaaggaaac tattgccata attcaggggg
     1561 aagtcatcag cggaggtgaa gtggaccgag gacaatttac tgacctcgcc acttgcacag
     1621 gaggacttgg cggtagactg tcttcagctg gtgctgcagc aatttcagat gaaacaatta
     1681 ctttggatag ttggagaggt tatgtttgtc ggaggggagc ttttgccagt aatgtagcag
     1741 aaatggcact ttcatcggca ttcgcgctgg cgggtttaaa tgaagttatt ccgcctaaaa
     1801 accaggttgt taggacatac aaatacaaga atatttccct tttcgcacag gcaaatttca
     1861 gttttactgt aataggcctg aagagggaaa cgctggcgat tcgtgcggat tcatccagct
     1921 ttggacgcag tattacggaa aaggattcct atcgcaacgt caaagaaagt cacctgattt
     1981 ccctgacttt aggtagcctg atcagttaca ccagaatccc aattttccca attccaattc
     2041 gtgaagttgc cttggcgagc cggagtaatc ccaaacagat attctatggc caaaaaccct
     2101 tgccggttcc aagcatttac gacttgcctc ccaaagaaaa gaagaaggta attcagaaat
     2161 tggtgatgct gacactgcag tcattcatcc gtacaatttc tccatacaag gtcatggttg
     2221 atgcagaggt cgcttccgga ggcaaagcaa aagccggaga tttactcaaa gagggaaaat
     2281 ccaaacaagc ccgaaagata attgaagcaa ttccggaagg aaaacgcgat tcgcaagatt
     2341 ggtataattt gggtttagca tatgaagcca gtgctgttag tccggaagat tatgaagatg
     2401 cacgacggtt ttatattact gcccttgaaa atagtccggg gacaaaactt tacgcacaag
     2461 gtgttggacg cacacgtctt tatcttgctg aaaaaagaag attggccaaa caaattcaag
     2521 attaaagcaa gaatttaatt tatgaataat tatatattta atcaaacttt gtacattttg
     2581 ttgataggcg cttttctgtt gacgagttgc gctaaagcta cgcttactgt gcgctcacct
     2641 gcggagatca acacaagaga catcagaaat atagccgtgg gtaaatttga gattgggcag
     2701 atttctctga aatataaaac tgagcggaat ggcatttggc aaacccatcc tgttttgctg
     2761 aatgaggccc aacaaaaatc catcagccgt tctgtcaggg cgcgggtaat aaatctctta
     2821 accgctactc cgtacttcaa agttgttttt tcagatgaat ttgaaaagct ggaaaatgat
     2881 acggcactgc agcaattagt ttcagtacgg ggatacaaaa ctcaaaatgt tgatgcagtt
     2941 atcagcggaa aactttggct cgaaattgaa cgaactgatg gagttgacct gagtaaagaa
     3001 ggcctggaat atttccggcc accacgttca cgacgtagcc tcggcttgaa tttaacagtc
     3061 gatcaagttg tttggtggcc ctacaaatca acacgcggga cattgggact tgaaataaaa
     3121 atgactcgtc tggtacctgc agaagttgtg gcaactactt ttgagactcg tacttactca
     3181 caaaggatag gtggaagaag cacagaaagt tttcagcaaa ttgcagaaag tttaacatcc
     3241 gttctgccaa cttcaaaaac caccgagact gaatccattg aaaaatctgc agatgttctt
     3301 ccttcctttg agcaaataat tgcggacctg gcactttcca tagccagcgg atttgtacgt
     3361 agagttgcag tcactgacaa aactgtcagc tacccaattg ctgatggtaa gtttccaaac
     3421 tctaaaattt tgatagaggc cggtgcttac gatttggcaa ttgaacagtt acaacaagct
     3481 actgctgaaa atccggatcc aaatgatctt tataacctgg gactttgctt cgaagcagtt
     3541 ggagattatg gactcgctca gacaagttac agagaggcgt ggagtgctga accggaaaac
     3601 ctgctttttg cgcagggaat gggacgcatc gaaagacttc agcgtgaaaa cccgcaactg
     3661 ctaagacaac tcgaatcacg ttaatgatta gggaacaatc tacaactgct cttatgttat
     3721 gaaactcata tcctttcaaa tgattcgctt ctttaatttt gcaatgcggg aagacattga
     3781 gactacttac atcaggactt cagtgctttt gagcatttgt gtatttttac tcacgggatg
     3841 tgcaccaaaa gtaagtttcc aaatcgaacg tccagcagct caacgtgttg aaaacattaa
     3901 ctacattgaa attggtaaat ttaaaattgt atccggacaa atcaagttgc caagttcggt
     3961 agaatccgga tcaacccaga gtttttcaag cactgataaa ttgttaaaac caaccatctc
     4021 caagttcaat tcaaacaaag aacaggcgtc tcaaattgca gacttgctaa gggctgctct
     4081 tgttcacaac ctttctcttc attcgccgta tcagttgatc aacacaaccg gaaaggaaac
     4141 cggatttagc ggtgtacttc cggatgcggc agaagttgca atcctgcaag gaaaagttaa
     4201 atattttgag cggataattg aatccgaaga ggctcttcct tattttacaa atatcaaaaa
     4261 caagggtgcc acccttgagc aatctttact ggcaagtgca gtttcaatgg gagcagaatc
     4321 ttctggagca ggatttttga ttccgactcc ttatgtggaa caattagccg cgcttgaagt
     4381 tgaatttact ttaataaata aaagtgatgg aagcgatgtc gttccccctc aaataattcg
     4441 cacttactat gtgcggaagt ggggtggtgc tcccgaaacc tcacatttac cgagaaatat
     4501 aaagacagca attatagaag gatttgaaca ggatgaagat aattctgagt cttttctttc
     4561 aagaattgac cgtgctggtt tgtcctttac aaaccctact gaatattttg caagaggatt
     4621 caacctgaaa cagaatgtcc aggttccaca aacctctttg gatttgaaga tcaaattggg
     4681 acgtcaagtg gcccaaacat atgtcaaaca aatcagtcct tttcatgaaa ctgcagattt
     4741 actggttcaa gatgggaata cagttgcatc gacactgatc aggggaaatg cctaccagga
     4801 agctgttgcc tatcttcagg gtttggagga gcttactgca aaagacgaat acaaccttgg
     4861 tctagctttt gaggccagtg gagaaattcc acaagcgagg aatcactatg aactcgcttt
     4921 gaaacgagaa acaagtaatc aagattttaa agatgcagta aaacgaacac gaaactaaac
     4981 tgtgtgctct aaaaatgtgg atttccagtt ggtttatttt aggtccgcca attcctgatg
     5041 gggatcattc ttcatatcaa agaatgggga tggaattttc aatgcacgtg aaatacgctc
     5101 aagagaggct tctgtaactt cagctgcatg ggattcccag ctttttacag tttgaattgc
     5161 aaaaccatgt tcataggcaa atgcctcaat cgacatgcct ctcagcaagc gggcatattt
     5221 gatgcgatca cctattctgt caagatcaag gtcattatcc aaacccatct cccgcacatc
     5281 aattgctaaa actttggcaa tttcactata tttagtcagt ttgggcgtag agtgtccagt
     5341 ttcccaattc tttacagcag tcagcgaaac actgagtttt ctggcaaatt ctttgcgagt
     5401 caaaccagag gtttcccgcc attgagccat ttgttcatca aattccattt tacttttttg
     5461 ttaattcttt atttttatac aggaaaatgt ttttcactca atttatccaa aaactcttat
     5521 ttttcaatat attgagctga aatttttgta caattaaagt gtcatctttt ccgttgaata
     5581 acgttgatta tcaatcaatt tgtcaagaat gtcaaccatt ttttggaaat gcttttttcg
     5641 gtcttcaaat gaaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgga
     5761 agaagcggag agttttattc agcagcatgc agcacgttat cagtcaatgt cttttgatga
     5821 acttgaatca aaagcattta gtcttattga agcgcatgaa aagttgatgg accagcaaag
     5881 cattgtactc tatgcgggta cgaatgtaat caatccaaaa gctgcaaaaa tgctttcttc
     5941 aagtatagga aacagagcca gtctgggggg tatcccggag caaagtataa caaaggcatg
     6001 gagcacgctg atcaattgga aattatgctc atgtcactca tgcgtcaatt gtttcaggcc
     6061 aaatatgtgg agtacagagt gccaagcggt tcgattgcaa acctctatgc gtacatggca
     6121 acaactaaac cgggtgacaa aatcatgtct ttttcggatg ctgcggcagg gcatgtgacc
     6181 catcatgcgt aagttgctgc cggactttat ggattggaaa ttcacgaagt cccttttgat
     6241 tttgcgcaaa tggatgttga tccggaagcc ttaatgatcg cagcgaaaaa agtgcgacct
     6301 aagctgatca ttattgctgg aagcatgtgc ttatttccct actcgctgca ggaagtccgg
     6361 aaggtagctg atgaagttgg ggcgtggatt ctttatgatg cagcccacat gggtggtttg
     6421 attgcaggtg gagaatttca gcggcctctc aaagaaggtg cggatttaat gaccggctca
     6481 acatacaaaa gttttggagg tcctccctcc ggaatggttt ttacgaattc agcagaactg
     6541 gctaagcgtc ttgacaaaat tgctttccca ggacttacag caaattttga tctcagtcgt
     6601 gctgcagcaa tggtcatagc cgttttggat ttgctgacac atggaagaga atatgcgaaa
     6661 atgtgtattg ccaacgccaa agcacttgca gaatcacttc acactggagg atgtgaagtg
     6721 tttcatgtaa gtggaaaagg cttcacgaat tcccagcatg tggctctgcc tgcagcaact
     6781 tatggtggag gagatactgc ctcaaagctt ttggaaaaat ccaatctctt caccagtgga
     6841 attggtttgc ccctgcctcc tgttccggga gactttaacg cgatgcgtct ggggacccag
     6901 gaaattacac gttggggaat gcgcccggaa aatatggaaa cgattgccga ctttttttgc
     6961 cgcctgctgc tgaaacagga aaaaccggaa aagctgaaat ctgaagtaat tgaattccgg
     7021 aaagcatttc aaaaactgca ctacattcga gactgattaa attctgactg ttactaaaat
     7081 agtcagatta atatatgttt gtcagtccga cttttttcaa taactcagtt tccagttcca
     7141 gcatttcact ttcttcctcc gaagatcgtt cgtgattcca gcctgccagg tgagcacagc
     7201 catgcgccag taagcggata agttccgtct caaaatccca tccattttca gtagcctgct
     7261 gactgacacg ttctccagaa agaaccagtt ccccggctac ccctgtactt tctgagtttt
     7321 ccaattcagc ctcactcatt tgggcttcaa acattcccgg atcatcttca tcataagccc
     7381 atgacaaaat atctgtggga caatcttttc cacggaattc cctgttcagt ttttgaatta
     7441 ctgcatcatt tgcgatatat aaagaaattc cacgttctcc tgcatacaaa tattttagaa
     7501 aaattgttag tattttctga agttcggatt tgttaatcaa gttttcccat ttttcatcac
     7561 agcgaatttc aatcatagga ctgttttttc aaatgcaaaa actcgcaagt gggcactccg
     7621 gcttctggga ttatcggcaa cctctttttt agtagcattt acaggccttc tggtcaggac
     7681 tcgtccccgt ggtttttcac cgcaaatgca gtatggcaga tcggttggac aaacgcaggg
     7741 attttcccat ttcctgaact ggtgtttgac aatgcggtcc tccagagaat gaaatgagat
     7801 taaggccaca cgaccgtcct tttttaatcg tgatggaatc tgcttcaaca acacttcaag
     7861 ttcttgcagt tcgtcgttaa cagcaattct cagggcctga aaagtcaaag ttgcaggatt
     7921 aaacccctgt ttttgcaacc gccttgggat tacactgcta acaagttggg caagctcctt
     7981 tgttgttttc aatgggtttt tttctctgaa ttccagtatc ttgcccacta ttttcctggc
     8041 aaaacgttct tcaccaaaat ttcgcaaaat cttgagcaat tcttgttcac tggaattatt
     8101 gacaaaatgt gcagcagtga tctgctgacg atccggatcc attcgcatgt caagaggtcc
     8161 ttcccgcaaa aaagaaaatc ccctctcagc acgtaccagc tgctcacttg acaggcctaa
     8221 atcagccaaa atataatcaa aaacaggctc accgcactcg gcaaaaaatt caggaagttc
     8281 agaaaaactg gcctgaccca gtttggtttg cttttcgtat gtcttcaatc tttccgctgc
     8341 agcttttaca gcaagcgcat ctctatcaat tccgtacagc tttgcgtccg ggaaattttc
     8401 caacaagcac ctgctgtgtc ccccgccgcc aagagtacaa tccagaattg tttgtgttga
     8461 ttccggggca aaatcaacca cttcttgtgt taaaactgaa atatgttcaa actgcatttt
     8521 catttaaagc gtaacatttt ttttgctgca gcatgataag cgtaaatctt tttaaaagct
     8581 atgttgtttg tacggagtaa actttgaaaa agtacattcg ggttaagagc tttgctttgt
     8641 gcaaaaatca ggttcagcaa tgtttttgcg tgttcttgag aaaaatccct gcgtaccacc
     8701 aaaacaggat accaagccac tccctccaat tcttctgttt gataatgata tttattggca
     8761 ggaagttttt gtgtaatcag ccaggagaat tttaaccgca aagcctgaat caactgctga
     8821 tttaatgata aaagccggat tcccggaaac tgttccagcc agtcgttgac ttctttcaat
     8881 ggagcaggac cggtgaaagc aagcacttct tcagaaaacc cttctgaaaa atttggaatg
     8941 ctctcagtat tgaaccagtt aatttttgtt tgcggtgcta aattcttcca gacttgcgcg
     9001 aaataaaacg aattatcatg tacaagcaaa gtttgggtgc tttcaagtga gagtagaggt
     9061 ccgggaactg tacccatgac atacaaccag ttttgccaaa gaactgtaag aacacgcaaa
     9121 cctgggtgtt tttttaattc tttgtgtgcg attgcagcat caataatcgc cagtgtaacc
     9181 ctgttattct ctaattgtgt cagacggctc tcgggcgaaa tttcaggatg tatcacgaat
     9241 tctattttgc gactggtttt ggatgaactc caaagacgcg ccagctcatt agcaaaaata
     9301 tatgatccag tattttgact accggtaaga attatatttt gctgctggcc ccacaaagtc
     9361 actgaactgg aacagagcaa caaaatttga ataaatattt ttaagaatag cttcatataa
     9421 aatatttttc accgttggca ttccggacaa aaaaaagtgc tcctttgctg ctgcactatt
     9481 cgttgaatag gcgttccaca ttctgtgcag ggctgttcct ttttatcata aacctgcaga
     9541 aattgttgaa atgccccagt tttatcatca actctgcgga aatcagaaat agttgtccca
     9601 ttattcttta tcgcttcttg caggatatat tttgtgtatt ttataatcaa cgaccattct
     9661 cttttattga tgctacccgc acttcgctga ggatgtatca tggagcggaa caatatttct
     9721 gaaacataaa tgttgcccag tcctgcaatt atttgttgat ccagcagtac tgatttaatt
     9781 tcccgcttgg aggatccaag attctttttg aaataaccta gagtcatagc tttcgaaaga
     9841 ggttcaattc ccagtttttg caaggacttt gagtcagcta aatcatcata tacttccaga
     9901 gttccaaagc agcgaatatc atcaaatatc atctgccgtc cactttctaa gttaaaccag
     9961 acacgattat attttgtcgg ttccggtaga ggatcactga ctatgaattt cccagtcatt
    10021 cgtaaatgag caactaaata tctttttggt tctaaattaa aaatcagata tttggcccga
    10081 cgcctaacat ccataaattt tcttccgcac aattgttcct ggaacgtatc caggtctccc
    10141 tgcacaattg gattactgcg gaatatttca actgatgaaa taatgtctcc acatatttct
    10201 tcacgtaatt ctctaacgac agtttcgact tcaggtagtt ctggcattta aatctttttc
    10261 atgcttgatt gatttctctt cgtgcaggat attctatctg agtcagattc gacactcaac
    10321 ccctttccgt agaggagttg ttattactat taatttaact aaacatttga ctgaattatt
    10381 tatagatata attatactct gaccccattt aataatcact atacgtcctt ttcgcattaa
    10441 agaagtattg caaatgacgc aggcaaagtt ttcttggaat tttgtgttaa catcgagttt
    10501 gtgtgataat acgctgattg atattgtatt tgataaactg tctagtaccc tctgattatt
    10561 ttgtgtattg atcagaaaaa atattcgtag aacaattatg cctgtttata atatttaatt
    10621 atcattaaaa aagaaggaac caattgagtc gtgcatatta ttcatcaact gttgaaaaat
    10681 tattaactga tgaacaggac ttaattcttg gtgaattaac atattttcgg gattattatt
    10741 ttaggtcaaa atggctgtag gatgggcccg tgaaggggcg gtacaggagc aaattgaggc
    10801 tacagttgaa gatgctgttc aattagcacg aagtcgctta ccagaagggg aaagttttat
    10861 caaatgtgag gaatgcgaaa tcgaaattcc ggctgcccgg cggaaagcag taccgggtgt
    10921 acgactatgc attcaatgtc aggaagaatt agaaaaaaag cagaccgcga tcagttatta
    10981 caatcgaagt gggaataagg acagccaact gaaatgatgc ccactattgc gggatgttaa
    11041 gcattaatca agagcgaatt ttcccccatg gtgtcccctg cagtctctgt ccggcaagcc
    11101 acaaacccag aatcccgcat ataactgcgg tagcgagcgg agcaatcagc cagattgtcg
    11161 gatctatgc
//
LOCUS       DTVD01000046            2851 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14292_length_2851_cov_352.691702, whole genome
            shotgun sequence.
ACCESSION   DTVD01000046 DTVD01000000
VERSION     DTVD01000046.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14292_length_2851_cov_352.691702"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="EYO68_01700"
     CDS             <1..568
                     /locus_tag="EYO68_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIC06549.1"
                     /translation="AAGDATKGKAAYAVCAACHGANGMGNKALNAPKIAGQEPWYLER
                     QLKNFKAGVRGAHPKDPYGMQMRPMALTLANDQAVSDMAAFLSSMPVSKSSESSVKGD
                     VAAGKTAYMICQACHGPTGGGNKALNAPRLTGLQDWYVVRQLKNFKSGARGTKAGDTY
                     GMQMRPMAMTLANDEAINNVAAYIATFK"
     gene            687..1754
                     /locus_tag="EYO68_01705"
     CDS             687..1754
                     /locus_tag="EYO68_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06550.1"
                     /translation="MQIFLEKRVNATSWARLLVLGAALLLVLLISWFFFLAAGIEPMT
                     AFQIIGEEIFLTKYGWQDLLVKMTPLMFTGLAVALAAQMQQWNIGAEGQFHMGTFAMT
                     WVALHYDGQLPDGMLIVLMLIMAMIGGGFWGCIPGFLKARFGVNEIITSLLLNYVAAA
                     WIDYLLFGAWRDPGTNNFPITKEFSEAGQLPSFGNTSIHAGLILAFVFIAIVAWVLWK
                     TKLGFQIRLTGDNPDAARYSGVNIRTLTILVFAASGAIAGLAGFSEVAGIHYRLQQNI
                     SIGYGYTGIIVAWLARNHPLGVVLSAFFMSIVFVSAEVLQIEFGLPISMVYLYQGIIL
                     FTVLGSDIFTEYRLRITRFLV"
     gene            1885..2814
                     /locus_tag="EYO68_01710"
     CDS             1885..2814
                     /locus_tag="EYO68_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06551.1"
                     /translation="MEFFIQILIAAVAMGTPLLFATLGGVISERAGVINLGMEGLMLV
                     GALVAFVVMLYTGNYFYAVAAAAFASGIVSMIHGVVCLMLRASQIASGLALTFFGTGL
                     SGLFGDKLMGETVEPLSRIPIPGLHTIPVLGPVLFNQDVIVYFSYLCVALSWWLLFKT
                     RIGLNIRSIGEAPEVCDSLGLSVLSYRFFAVVGGGMLIGIGGAYFPLALTPFWVDGIT
                     AGRGWIAVALVIFAFWDPLKALGGAYLFGLALALELRLQILGIDMSPYFLKMLPYVLT
                     ILVLTLVTIRNNRRGIQVMPAALGKVFFRGEKH"
BASE COUNT          704 a          553 c          705 g          889 t
ORIGIN      
        1 tgctgctgga gatgcaacaa aagggaaagc cgcatatgcg gtttgtgctg cctgtcatgg
       61 agcaaacggt atgggaaata aagctctgaa cgcacccaag attgctggtc aagaaccgtg
      121 gtatctggaa cgtcaactaa aaaattttaa ggcaggcgtc cgaggtgcac atcccaaaga
      181 cccctatggc atgcaaatgc ggcccatggc tttgacctta gccaatgatc aggcggtttc
      241 agatatggca gccttcttgt caagtatgcc tgtgtcaaaa tcttcagaaa gttcagtcaa
      301 aggtgacgtt gctgcaggca agacagctta tatgatttgc caggcttgtc acggcccgac
      361 tggtggtggt aataaagcat taaacgcgcc ccgactcaca ggtttgcagg attggtatgt
      421 cgtacggcag ttaaagaatt tcaagtccgg agctcgtggg acaaaagctg gtgatactta
      481 tggaatgcaa atgcgtccga tggcaatgac tttagccaat gatgaggcta ttaataatgt
      541 cgcggcatac atagctactt tcaaataaca gaaaaccacc ttccttcttg aaaggttcct
      601 aaaaattgcg agcctttttc attcctgcaa cttagtttcc tgctctttaa aaaagtttta
      661 atattctaaa atccaagagc attagaatgc aaattttttt ggaaaagcga gtcaatgcta
      721 cgtcctgggc gcgattatta gtgcttggcg cagcattgtt gttagtcttg ttaataagtt
      781 ggttcttttt cttggcagct ggaattgagc caatgaccgc tttccagatt attggagagg
      841 aaatctttct gactaaatac ggctggcagg acctgttggt taaaatgaca cctttaatgt
      901 ttacaggact ggccgtagca cttgctgctc aaatgcagca gtggaatatt ggcgcagaag
      961 gtcagtttca catgggtaca tttgccatga cctgggttgc tttacattac gacgggcaac
     1021 ttccggacgg tatgctgatt gtactaatgc tcatcatggc catgataggc ggtggttttt
     1081 ggggctgcat ccccggattc ttaaaagccc gttttggagt caacgaaatc ataacttctc
     1141 tcctgttgaa ttatgtggcg gctgcatgga tagattattt gttgtttgga gcatggcgtg
     1201 acccgggaac caacaatttt ccgatcacta aagaattttc tgaagcagga caacttcctt
     1261 catttggaaa tacttcaatt catgccggac ttattctggc ctttgtcttt atagcaattg
     1321 tagcctgggt tttatggaaa accaagcttg gttttcaaat ccgcctgacc ggtgacaacc
     1381 ctgacgctgc ccgttactcc ggagttaaca ttaggacatt gactatcctg gtctttgccg
     1441 caagtggagc cattgcaggc cttgccggat ttagcgaagt agcaggtatt cattaccgtt
     1501 tgcagcaaaa catttccatc ggctatggtt atacgggaat cattgtggcc tggcttgcca
     1561 gaaatcatcc gcttggtgtt gtcttaagtg cattttttat gtcaattgtt tttgtcagcg
     1621 cagaggtttt gcaaattgaa ttcggtctgc caatttcaat ggtatatttg taccagggta
     1681 ttattttgtt tacagttttg ggttctgata ttttcactga atatcgactt aggataacgc
     1741 gttttttggt ttagccaaaa acacacaaaa gcacacaata aatggtacaa gaagaattag
     1801 tttataaaat caaacgtttc ataaaataaa tgtttttgtg tgtttttggc aaaatttaag
     1861 agttctaatt aaaaacaaaa agatatggaa tttttcatac aaattttgat tgccgcagtt
     1921 gccatgggaa cgccgttatt attcgcaaca ctgggtggag tgatcagtga gcgtgcgggc
     1981 gtgatcaatc taggaatgga ggggctgatg ctcgtgggag cgttagtggc ctttgtcgtg
     2041 atgttgtata ccggaaacta tttttatgca gtagcagccg cggcttttgc ttccggaatt
     2101 gtgtcaatga ttcacggggt agtttgttta atgttacggg caagtcagat tgcttcaggg
     2161 ctggcactga ctttttttgg tactggacta agtggtttgt ttggtgacaa actgatggga
     2221 gaaactgtgg aacctttaag ccggattccg atacccggac tacatactat tccggtgctt
     2281 ggaccggtgc ttttcaatca ggatgtcatt gtttattttt catacctttg tgtcgccttg
     2341 tcgtggtggc tgctattcaa aactcggatt ggtctgaaca tccgttcaat aggcgaagca
     2401 ccggaggtct gtgattcact tggactttcc gtgttgagtt atcgtttttt tgcagttgta
     2461 ggtggtggaa tgttgattgg aattggagga gcgtattttc cgcttgcatt aactccgttt
     2521 tgggtagatg gtattactgc gggacgcgga tggattgcgg ttgctttagt gatctttgct
     2581 ttttgggacc cgctaaaggc ccttggggga gcttatctct ttggccttgc actcgcactg
     2641 gaactgcgtc tgcaaatatt gggcatcgat atgtcaccat attttttgaa aatgctgccc
     2701 tatgttttaa cgatcttggt actgactttg gttacgatcc ggaataaccg gcgaggaatc
     2761 caggtcatgc ctgccgcact tggaaaagtt tttttcagag gtgaaaaaca ttgatcttat
     2821 gtgtggattc taccggcttt gtgccggatt c
//
LOCUS       DTVD01000047           11126 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1432_length_11126_cov_581.485141, whole genome
            shotgun sequence.
ACCESSION   DTVD01000047 DTVD01000000
VERSION     DTVD01000047.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_11126_cov_581.485141"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="xth"
                     /locus_tag="EYO68_01715"
     CDS             <1..722
                     /gene="xth"
                     /locus_tag="EYO68_01715"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIC06552.1"
                     /translation="FFEWLHTQKADLVCLQETRIQPEQLTEIMLNPPGLKSAFVFAAK
                     KGYSGVGLYFRKKPDRVHEGIGWSELDKEGRFIQADFGKLSIVSLYLPSGSSGDIRQN
                     FKYKVLDYLDKWLLEAKLSGREIIICGDWNIAHKEIDLKNWRNNRKNSGFLPEERAWM
                     TKIFSELGYVDVFRTLNPDPDQYTWWSNRGRAWDNNVGWRLDYQVATPGIGVKAVKQS
                     IYKKQRFSDHAPLIIDYDYRL"
     gene            complement(762..980)
                     /locus_tag="EYO68_01720"
     CDS             complement(762..980)
                     /locus_tag="EYO68_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06553.1"
                     /translation="MVNSLQPEYKGKIRFLVANLNSKEGRWFAEYHNVSRVTLLFFKP
                     DGTKISTLNGEQQPDFLRRVFDRVFKLE"
     gene            complement(1005..1205)
                     /locus_tag="EYO68_01725"
     CDS             complement(1005..1205)
                     /locus_tag="EYO68_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06554.1"
                     /translation="MSKHRRVKGSSKKAHRRQNVRWLVSITSVLILVLAGWIWLDSTE
                     EDNDLSAIGKGENVVVQIHDPG"
     gene            1312..1719
                     /locus_tag="EYO68_01730"
     CDS             1312..1719
                     /locus_tag="EYO68_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06555.1"
                     /translation="MEIITWGDIRRGTLAIVLVIVPTVLLGTYMAPALQSYQITGPGL
                     WELKRKGAPDALIEPLHELRLKPFFFKFRLMSEVESRISDADLFKKYEKKIERQISGI
                     AMPAVEYIFLAVLAAYYWILWFVTRRWFPDGKV"
     gene            complement(1834..2406)
                     /gene="scpB"
                     /locus_tag="EYO68_01735"
     CDS             complement(1834..2406)
                     /gene="scpB"
                     /locus_tag="EYO68_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIC06556.1"
                     /translation="MTKLEQKQALETILFVATKSLSPKQLAEMIGGCETGLVRELVSE
                     LNAAYEKTDRVFRIDSVADGLQMHTLPEYKKWAQSLETVKVIKLSPAVMETLAIIAYK
                     QPLTRAGIEFIRGVDSSHTIRRLMQQKLVRIVGREMLPGRPGLYGTTKGFLEVFGLTR
                     LKYLPALSELDMDKLPEESQLELPLESEDE"
     gene            complement(2427..2687)
                     /locus_tag="EYO68_01740"
     CDS             complement(2427..2687)
                     /locus_tag="EYO68_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06557.1"
                     /translation="MNKRRLGTILIAGSVLLWLINRFSYIISSYFSRLLCGELYLQPV
                     DGILGDVSCGFNADMHFTALMFLVLITGIAVLIISLVQKDVH"
     gene            2801..3211
                     /gene="queF"
                     /locus_tag="EYO68_01745"
     CDS             2801..3211
                     /gene="queF"
                     /locus_tag="EYO68_01745"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013507005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HIC06558.1"
                     /translation="MDTSSIDYAEGRVFTFEDESAIRPGFLETIEYSGPRQHVSYQMN
                     EFAAVCPFSGLPDTGIVWVDYVPKQKLVELKALKYYFLSFRNVGIFQEAVTARIFKDL
                     YPLLEPEELLIKTRYNTRGGIDTTCTIDSNEQKN"
     gene            3195..4400
                     /gene="metC"
                     /locus_tag="EYO68_01750"
     CDS             3195..4400
                     /gene="metC"
                     /locus_tag="EYO68_01750"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HIC06559.1"
                     /translation="MNKKTDSSVLREDTKVVVSGRDIKNYGGVINPPVYHGSTILAES
                     VESYNSRYLEAKEGEQVMVYGTMGNPTAWALENAIAELEGGYHCSTYPSGLAAISVAL
                     MAFLKSGDHLLMTDSPYAPTRRFCDTVLRRFGVETTYYDPLIGAEIESLMRPNTAVIF
                     TESPGSNTFEVQDIPAIAEVARKHGAITQLDNTWASPLYFKPFEHGVDISIQAATKYI
                     VGHSDVLLGSVTTTRENWKILRDCTWHFGQCVAPDDLYLAQRGLRTMAVRLRQHQKSS
                     LEIAEWLSNRNEVRQVLYPALPDAPGHEIWKRDFLGASGLFGVELETFSQKAVEKMID
                     EMRLFGIGSSWGGYESLILPVNVRDLRTASPWRFEGPLLRLHIGLEDVDDLKDDLNDG
                     LERLRNFQS"
     gene            4446..5330
                     /locus_tag="EYO68_01755"
     CDS             4446..5330
                     /locus_tag="EYO68_01755"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC06560.1"
                     /translation="MNFLVADDSVEVLECLRKFLEKEGHSVRCTGDGEEALQIFRGEE
                     IDAVFCALTLPKLGGLELLQEVKSANSRRPFVMICPQNDTESALNALQLGVCDFLIKS
                     IQPLELQRTLDRVVSLNEGFHSNAYAMDHLLQESRTLEIGNDFEDVNRIVAFMTQDLP
                     SFGILEQSQLFRMNVMLKEALENAIFHGNLELNPEMRRDNPILFYETAKRKMDIDPYN
                     NRKLLLQIDISRNSAKYLVRDEGKGFAHSDLLDPKDPENLPRIKGRGLLMIMNFMDEV
                     FWNDRGNEMTMVRYRKRN"
     gene            5330..6151
                     /gene="bamD"
                     /locus_tag="EYO68_01760"
     CDS             5330..6151
                     /gene="bamD"
                     /locus_tag="EYO68_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIC06561.1"
                     /translation="MSKVSVLDIVKMKCFTSLKWNIFCFQIFILLLFSGLLQSCSETA
                     NVQIRLPAKELYQKAMVAVEDEFYQEALKNFEILVDEHSGTRLATLAHLKMGEVYFLQ
                     SKWEESETSYRRFLLLNPRSHLTPYVLNRLIALNYERNIYGLFAKSRDYDRNMEPNRT
                     LIREYQRFYLLFPQSPYLADVKEYQKGALADLAEHELHVANYYFDNEAYHSAIGRYLY
                     LLKNYPGFPRTGDVAERLIEAYRLNQQPELADEMQKILKSLRERKLLAQHTTREE"
     gene            6148..7335
                     /locus_tag="EYO68_01765"
     CDS             6148..7335
                     /locus_tag="EYO68_01765"
                     /inference="COORDINATES: protein motif:HMM:PF13174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC06562.1"
                     /translation="MTKPSVRFSLGRYLILLAGIQFFFSGCAQLDGIFPKFKPQETTK
                     KSTSTSSAETVTEQQEILTEENRLLKAKIEQLAQQISELQKQQKVQSDDFLLLQEQWE
                     TNFVLLERSVEESLRSAKASESTALMITQVLENQSLNSSADRSGTDSQGSLPPLENYS
                     LLGKPETSVQKASAVATTKEMEKINNLEISEQEMGVEEEPSFPEAGLVNMQDLEDPED
                     LPDPDVRAIQVEIAGSETSQESVKTVETSDNGFSDPDLNPPEDPFILIRHPGVKKIYN
                     QGMTAVIQKNHEQAILIFENFTERFPDNLDSDNAFYWIGRSHLELNDLKKAETAFRNV
                     LRYYEHRPTSQGYKTPDVIYMLGKLQEKQNLDQRAAYYFGEVIKRFPGSAAARNAKLD
                     LDR"
     gene            complement(7337..7594)
                     /locus_tag="EYO68_01770"
     CDS             complement(7337..7594)
                     /locus_tag="EYO68_01770"
                     /inference="COORDINATES: protein motif:HMM:PF02325.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIC06563.1"
                     /translation="MPISGTLVQVLAAMLRLYSLMLVVYALLSWVMHEQHYPPAIRWL
                     QSMVEPVLSRIRKVIPPIGGIDVSVIAALLLIEMIRSFILY"
     assembly_gap    7721..7820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7890..8990)
                     /gene="lpxD"
                     /locus_tag="EYO68_01775"
     CDS             complement(7890..8990)
                     /gene="lpxD"
                     /locus_tag="EYO68_01775"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:PRK00892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIC06564.1"
                     /translation="MRYMAAVSYSLKELAEQLGLKFSGNGDLQITHVCGLDSLQAGGL
                     AYLTSPDGLTSVPTPAGMSRHVGTTVDEINSSKVALVVSPDVQHPEHNLIYADDPLAA
                     HVEATKLFHPAEPFIPDVSMKSGIHPSAVVSKSAKLGKNVKIGPNVVIYDGVNIGENT
                     ILHAGVVIMSEVVIGADCTFFPNTVVREKCEIGNRVILQSGVVIGSDGHGFFQRGGIN
                     LKIPQVGTVCIEDDVEVGACTTIDRARFRTTVIKRGSKLDNQVQIAHNVSVGEQALIS
                     AQTAVGGSVKAGHHLIMGGQSGIRDNVIVGNNVTAVARAVITANTQDNEVLGGMPGRP
                     VNQWRQLQSLINRLGELFERVRKLESRKDSSQ"
     gene            complement(8987..10207)
                     /locus_tag="EYO68_01780"
     CDS             complement(8987..10207)
                     /locus_tag="EYO68_01780"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03454.13,HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIC06565.1"
                     /translation="MMLSYEDARSLVFEHVKPLGKRNCPLTESQGLALSEDILAPHGL
                     PLFDNSAVDGYAVQSADLVDASRKNPVRLKKLGYIAAGDTGEEQMNSGQCLQIATGAP
                     LPPGADTVVMKEDIELNESFVYFTQAIPKTQNVRNQGEDIPEGRIMIPAGTLIGPAQI
                     AVLATFGYAQVPVHRLPKISIVSTGSELVDVEGKPQAGQIRESNRFMLAGMVRQESCE
                     LVKISLVPDIPEILIQAFEEALQTDVALISGGMSVGDKDFAKPLLKEMGVEEIFWKIK
                     FKPGKPLFFGRRGKTLVFGLPGNPASSYVLFEEFVRPALRKLMGRKILEETMVKATLD
                     EPITETLNRLHFMRGQLSEKNGKFRVRPLAFQGSHSISSLVESNALIRVEADSPVLPE
                     GSQVLVRPLRNEIG"
     gene            complement(10223..10420)
                     /locus_tag="EYO68_01785"
     CDS             complement(10223..10420)
                     /locus_tag="EYO68_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06566.1"
                     /translation="MIMPAKIKNRFPKKELNAWLRVHQTWDHIEWLNLLENLTKLGFH
                     EWSTSGLGQREIGFYLETKRH"
     gene            10603..11016
                     /locus_tag="EYO68_01790"
     CDS             10603..11016
                     /locus_tag="EYO68_01790"
                     /inference="COORDINATES: protein motif:HMM:PF03653.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06567.1"
                     /translation="MYIFALTLHVIAVMLVIGTLFVQSLSVVFRLRLSNQVQIEGIQF
                     VQRRVHRLIYYPVLAVAVASGAYLAVIQERISSPGNGWLHTKITLLLILIILGFVNRR
                     QIMNIDLRKPQALLVHIGIFFVSAAMIFLAQAKPF"
     gene            11038..11126
                     /locus_tag="EYO68_01795"
     tRNA            11038..11126
                     /locus_tag="EYO68_01795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:11072..11074,aa:Ser,seq:cga)
BASE COUNT         3001 a         2422 c         2463 g         3140 t
ORIGIN      
        1 gtttttttga atggctacac acacaaaaag cagatctggt ttgcctgcag gaaaccagaa
       61 ttcagccgga gcaactaact gaaatcatgc tcaatccacc aggtttaaag agtgcttttg
      121 tatttgctgc aaaaaaaggt tattccggag ttgggcttta tttccggaaa aaaccggatc
      181 gagttcatga aggtatcggc tggtctgagt tggacaaaga gggccgcttc attcaggcag
      241 attttggcaa attgagtatt gtctcacttt atctaccctc cggaagcagc ggagatattc
      301 ggcaaaattt taagtacaag gtgctggatt atttagataa atggcttttg gaagctaaat
      361 tatccggaag ggaaattata atctgcggag actggaatat tgcgcacaag gagattgatt
      421 tgaaaaactg gagaaacaac cgtaaaaatt ctgggttttt acccgaagaa cgtgcgtgga
      481 tgacaaaaat attttctgaa cttggttatg tggatgtatt ccggacactt aatcctgatc
      541 ccgatcaata tacctggtgg tcaaaccgcg gtagagcgtg ggataataat gttggttgga
      601 gactagatta ccaggttgcc actcctggaa ttggggtcaa agccgttaaa cagtcaattt
      661 ataaaaagca gcgtttttca gatcatgccc cactgatcat tgattatgat tacagacttt
      721 aatcacattg aatttgtata acagacgttc tccctcctat gctattccag tttaaatacc
      781 cgatcaaaga cacggcgtaa aaaatccggc tgctgctcac catttaaggt gctgattttt
      841 gtgccgtccg gtttaaaaaa gagtagcgtg acccggctga cgttgtgata ttctgcaaac
      901 cagcgtcctt ctttggagtt cagattagca accaggaaac gtattttccc tttatattcc
      961 ggctggagcg agttgaccac ccgcttgagt gcacggcagg aaggtcaacc ggggtcatga
     1021 atctggacta ccacgttttc tcccttgccg attgctgaca ggtcattgtc ttcttctgtg
     1081 ctgtcaagcc agatccaacc ggcaagaaca agtatcagca cgctcgtaat ggaaaccagc
     1141 caacgaacat tttgccttcg gtgagctttt ttggaagaac cttttaccct tcgatgtttg
     1201 ctcatacaaa tttcctttca gttaactctt caataatctg gattgatcat tgtgtcaatc
     1261 atgctacatt aacctgaact taaaatacac acaaaccatg aacaggtttt tatggaaatt
     1321 attacatggg gggatatccg tcggggtaca ttagccattg ttctcgtgat tgtgccaact
     1381 gttttgcttg gaacatatat ggctccggca cttcaaagtt accagattac agggccggga
     1441 ctgtgggaac tgaaacgcaa aggtgctccg gatgccctga ttgaaccact gcatgagttg
     1501 cggctaaaac cattcttttt taagtttaga ctaatgagcg aagtggagtc cagaatttct
     1561 gatgcagatt tattcaaaaa atatgaaaag aaaatagagc gtcaaatctc aggtatagcc
     1621 atgcctgcag ttgagtatat ttttcttgct gttttagcgg cttattattg gattttatgg
     1681 tttgtaacga ggagatggtt ccctgatgga aaggtatgat taagttcacg ggttttttgc
     1741 ctttaaaacc gttgcttttc aaaggtgaaa tctctcattt tggtttcccg gatgaagatc
     1801 cacgctttca gggtcagctt gaaatctgtc actctattca tcttccgatt caaggggtag
     1861 ttcaagctgg ctttcttcgg gaagcttgtc catgtcgagt tcacttaaag caggcaggta
     1921 tttgagtctg gtgaggccaa aaacttcaag gaaacccttt gttgttccat aaagtccggg
     1981 ccgtccaggt agcatttcac gacccacgat ccgcaccagt ttttgctgca tcaggcggcg
     2041 gatagtgtgg gaagaatcga ctcctcgaat gaactctatt ccagcccggg taaggggttg
     2101 tttgtaggca attatagcta aagtttccat gactgcaggt gaaagtttga ttacttttac
     2161 tgtttctaga gattgtgccc attttttata ttccggaagt gtgtgcatct gcaaaccgtc
     2221 tgcaacagag tcaatccgga aaacacggtc ggttttttcg taagctgcat tcagttcaga
     2281 aaccagttcc cgaacaagtc ctgtttcaca accaccaatc atttccgcaa gttgtttggg
     2341 agatagtgat ttggttgcaa cgaacaatat agtttcaaga gcttgttttt gttccagttt
     2401 tgtcatagaa ttccaatcag taaatattaa tgcacatctt tctgtacgag tgatatgata
     2461 aggactgcaa ttccggttat caaaacaaga aacatcaaag cggtgaaatg catgtctgca
     2521 ttaaatccac aggaaacatc accgagaata ccatccacag gctgcagata aagttccccg
     2581 cataaaagcc ggctaaaata agaagaaatt atgtatgaaa acctgttgat taaccagagc
     2641 aaaactgaac cggcgatgag tatggttccc agccttcttt tattcatttg cccctttttg
     2701 tttgcacagc agcaaattat gcaagcagac atgctccgta gttaaaactc aacattatcc
     2761 cgattcttat ctataacgca accctttttt gagtaatttg atggatacat ccagtataga
     2821 ttatgcagaa ggccgagttt ttacctttga ggatgaaagc gcaattcggc ccggttttct
     2881 ggaaacaatt gagtattcag gtccacggca gcatgtaagc taccaaatga atgaatttgc
     2941 ggcagtttgt cctttttccg gtcttccgga cacaggaatt gtctgggtcg actatgtacc
     3001 taaacagaaa cttgttgaac tcaaggcact gaaatattat ttcctttcat tccgcaatgt
     3061 tggaattttc caggaagctg taactgccag gatttttaag gacttgtatc cacttcttga
     3121 accggaagaa ttgctgatca aaacccgcta caatactcgt ggcggcattg acaccacctg
     3181 caccattgat tccaatgaac aaaaaaactg actcatctgt tctccgcgaa gataccaaag
     3241 ttgttgtttc cggacgtgat ataaaaaatt acggaggtgt gattaatcca cctgtttatc
     3301 atggctcgac tatcctcgca gaaagtgtgg agagctacaa ttcccgatat cttgaagcaa
     3361 aggagggtga gcaggtaatg gtttatggaa caatgggaaa tcccacagca tgggctctgg
     3421 aaaatgcaat tgcagaactg gagggcggtt atcactgttc aacatatcct tccggactgg
     3481 cagcaatttc ggttgcgttg atggcttttt tgaaaagcgg tgaccatttg ctgatgacag
     3541 acagtcctta tgctccaacc cggagatttt gcgacacggt tttgcggcgc tttggagtgg
     3601 aaacaacata ctatgatcca ttaattggtg cagaaattga gtctttgatg cgcccaaata
     3661 cagcggtaat cttcaccgag tcaccaggtt caaatacttt tgaagtccag gatattcctg
     3721 ccattgcgga agttgccaga aaacatggag caattactca gctggacaac acctgggcct
     3781 caccgcttta tttcaagcct tttgaacacg gagtcgatat ttccattcag gcggctacaa
     3841 aatacattgt cggacattcg gatgttttgc tcggatctgt taccactacc agggagaatt
     3901 ggaaaattct gcgggactgt acatggcact tcggacaatg tgtcgctcct gatgatcttt
     3961 acctggctca gcgcggcttg cggactatgg ctgtgcgcct gcgtcaacat caaaaaagta
     4021 gccttgaaat tgcagaatgg ctcagtaatc gtaatgaagt acggcaggtt ttgtatccgg
     4081 ctcttccgga tgctccggga catgaaatat ggaaacgcga ttttttggga gccagcggat
     4141 tatttggtgt ggagttggaa acattttccc aaaaggcggt ggaaaaaatg attgacgaaa
     4201 tgcgattgtt tggaattgga tcatcctggg gaggctacga aagtctgata ctgccggtta
     4261 atgttcgtga tctgcggact gcttcgccgt ggcgttttga aggtccgctg ctgcgtctgc
     4321 acatcggact tgaggatgtt gacgatttaa aggatgattt aaacgatgga ttggaacgtc
     4381 tgcggaattt tcaaagttga ttttcttctg cagcactact cacaacccaa ctaaaaaacc
     4441 tcataatgaa ttttctggtt gccgatgatt cagtagaagt cctggaatgt cttaggaaat
     4501 tcctggagaa agagggacat tccgtgcgtt gcacaggtga tggtgaagaa gctttgcaga
     4561 tttttcgagg agaggaaatc gacgcagttt tttgtgcgct gacactgccg aagttgggag
     4621 gattggaatt gcttcaggaa gttaaatctg ccaacagtcg tcgtccattt gtaatgattt
     4681 gcccgcagaa tgatactgaa agtgcactca atgcgttgca gcttggagtc tgcgattttc
     4741 tcattaaatc gattcagccg ctcgaactcc aacgtactct tgacagagtt gtttcactta
     4801 acgagggctt ccactcaaat gcttatgcta tggatcacct gctacaggaa tcacggacat
     4861 tggaaatcgg taacgatttt gaggacgtaa accggattgt tgcatttatg acgcaggatt
     4921 tgcccagctt tggtattttg gaacaatcac aattgtttcg tatgaatgta atgctcaagg
     4981 aagccctgga aaacgctatt tttcacggca acctcgaatt gaatccggaa atgcgtcgtg
     5041 acaatcctat actgttttat gaaacggcaa aacgaaaaat ggacattgat ccatataaca
     5101 accgcaaact tcttcttcaa attgatatca gccgaaactc tgcaaaatac ttggtacgtg
     5161 atgaaggaaa gggatttgca cactcagatt tactggatcc gaaagatcct gaaaacctgc
     5221 cccgcatcaa aggacggggt ctgctgatga taatgaattt tatggatgaa gttttttgga
     5281 acgaccgggg aaacgaaatg acgatggtcc gctaccggaa gcgtaactaa tgagtaaagt
     5341 ttcagtactt gatattgtta aaatgaagtg tttcacaagt ctcaaatgga atattttttg
     5401 tttccaaatt ttcattctgc tcttgttttc cggacttttg caaagctgtt ctgaaacagc
     5461 aaatgttcaa attcgtttgc cggccaaaga gttgtatcaa aaggcaatgg ttgcagttga
     5521 agacgaattt taccaggaag cattgaaaaa ctttgaaatc ctggttgacg aacattctgg
     5581 aacacgtctg gcaactcttg cgcatttgaa aatgggtgaa gtgtattttt tgcagagtaa
     5641 atgggaagag tccgagacca gctacaggcg gtttctcctg ctcaatcccc gcagtcacct
     5701 cacaccttat gtactcaacc gtttgattgc acttaattac gagcgcaata tctatggttt
     5761 atttgcaaag tcacgggatt acgaccgtaa catggaacct aaccggactc tcattcgcga
     5821 ataccagcgt ttttatttgc tttttccaca aagtccatat ctggcagacg tgaaggaata
     5881 tcagaaagga gcattggcag atcttgcaga acatgagctg catgtggcga actactattt
     5941 cgataatgaa gcttaccact cagcgattgg acgctatctg tatttgctca aaaattatcc
     6001 cgggtttccc cgcaccggag acgttgcaga acggctgatt gaggcctacc gtttgaatca
     6061 gcagccggaa cttgctgacg aaatgcaaaa aattcttaaa tctttgcggg agcgaaaatt
     6121 actggcacaa catacaacga gagaggaatg accaaaccgt cagttcgttt ttcacttgga
     6181 agatatctga tccttttggc ggggattcaa tttttcttct ccggatgtgc ccagctggat
     6241 ggtatttttc caaaattcaa accccaagag accactaaaa aatccacttc cacttccagt
     6301 gctgaaactg taacagaaca acaggaaatt cttactgaag aaaatcgtct gttgaaggca
     6361 aaaattgagc agctggcaca gcaaatcagt gaactccaaa aacagcaaaa agtacagagc
     6421 gatgattttc tattgcttca ggaacaatgg gaaactaatt ttgttttgct ggagcgttct
     6481 gttgaggaat cattgagatc agcaaaggcc tctgaatcca ctgctttaat gattacacag
     6541 gtgctggaga atcagtcgct taattcatct gcagatagat caggaacaga ttcacagggt
     6601 tcacttccac cgcttgaaaa ttattcattg ctgggcaaac ctgaaaccag tgtccagaaa
     6661 gcatctgctg tcgcaaccac gaaggaaatg gagaaaatca ataatctgga aatcagtgag
     6721 caggaaatgg gtgttgaaga agaaccgagt tttcctgaag ctggactggt gaatatgcag
     6781 gatttggaag atccggaaga tttaccggat cctgatgttc gtgctattca agttgaaatt
     6841 gcaggcagtg aaacttcgca agaatctgtg aaaactgtgg aaacatcaga caacggtttt
     6901 tccgatccgg acctcaatcc tccggaggat ccgtttattt taattcgtca tcctggagta
     6961 aagaagatat ataatcaggg tatgactgca gttattcaaa aaaatcacga acaggccatt
     7021 ttgatttttg agaattttac ggaaagattt ccggacaatc ttgatagcga caacgccttt
     7081 tactggattg gacgctctca cctggaactg aacgatttga aaaaagcaga aacggctttt
     7141 cgcaatgtac tgaggtatta tgagcaccga ccgacctcac agggctataa aactccggac
     7201 gtgatataca tgctgggcaa actgcaggaa aagcaaaatc ttgatcagcg ggcggcttat
     7261 tattttgggg aagtaatcaa gcgctttcca ggctctgcgg cagccaggaa tgcaaaactt
     7321 gatttggaca gatgattcag taaagtataa aagagcgaat catctcaatc agcaggagtg
     7381 ccgcaatcac cgaaacatca attccgccta tgggcgggat gacttttcta atccgactta
     7441 gcactggttc caccatgctc tgcagccagc gtatggcggg aggatagtgc tgctcgtgca
     7501 tgacccatga aagcaatgca tagacaacca gcatcaagct gtaaagccgt aacattgccg
     7561 caagcacctg tactaatgtt ccggaaatag gcattattaa ttttcctgtt tatttgttat
     7621 agtaacaatc tcgcaagaag ttgtcattct tgcggaagcg ggaatcaagg aactctgaaa
     7681 ctgtcccccg caacggagtg cggggctacc gtattctcca nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn ccccgcaacg gagtgcgggg ctaccgtatt ctccaataca
     7861 ataatgtacg ggcacggtgc gctgtatccc tattgtgaag aatccttgcg ggattccagc
     7921 ttacgtactc gctcaaaaag ttcgcctaat cggttaatga gtgactgaag ctgcctccat
     7981 tgattgaccg gccgtccggg cattccgccc aagacctcat tgtcctgtgt gtttgcagta
     8041 attacggctc gtgctacagc agtgacgttg tttcctacaa tcacattatc cctgattcca
     8101 gactgcccac ccataatcag gtggtgtcca gccttaacgc tgcctccaac tgccgtttgc
     8161 gctgagatca gggcttgttc acccacactt acattatgag caatttgcac ctgattgtcc
     8221 agtttactgc cccgctttat aacagtcgta cgaaatcgtg ccctgtcgat agtcgtacat
     8281 gcgccaacct caacatcgtc ttcaatgcac actgttccaa cttgcggtat tttcaggtta
     8341 atacctccac gctgaaaaaa accatgtccg tctgaaccga tcacaactcc ggattgcaaa
     8401 attacccgat ttccaatttc acatttctcc cgcaccactg tatttgggaa aaaagtgcaa
     8461 tctgcgccaa ttacaacttc tgacatgatt acaactcctg cgtgcaggat ggtattctcg
     8521 ccaatgttta ctccatcgta aatcacaaca ttgggaccga ttttcacatt tttgccaagt
     8581 tttgcacttt tactcacaac cgcgcttgga tgaattccgg acttcatgga aacatcagga
     8641 atgaacggct ctgcaggatg aaaaagtttt gtggcctcaa cgtgtgccgc cagaggatca
     8701 tccgcgtaaa tcaggttgtg ctccggatgc tgaacatctg gagaaacaac caatgctacc
     8761 ttgctcgaat taatttcatc tacagttgtc ccaacatgcc tggacattcc tgcaggtgtc
     8821 ggaacacttg ttaatccatc cggacttgtc agataggcca gtccacctgc ctgaagcgaa
     8881 tccaatccgc agacatgtgt gatttgcaaa tctccgttgc cggaaaactt caaaccgagt
     8941 tgttctgcaa gttctttcag tgaatatgaa acagcagcca tatatctcac ccaatttcat
     9001 ttcgaagggg ccgtaccagg acctggcttc cttcgggaag taccggagaa tctgcctcta
     9061 cccggatcag ggcatttgat tctaccagcg aactaatact gtgggagccc tgaaatgcga
     9121 ggggccgtac ccggaatttt ccattctttt cactgagctg tcctcgcatg aaatgcagac
     9181 gatttaaggt ttccgtgatc ggttcatcca gcgtggcttt aaccatagtt tcttctaaaa
     9241 tttttcgccc catcaatttt cgcagtgcag ggcgcacaaa ttcttcaaac aaaacgtaac
     9301 ttgatgcggg gtttcccggc aacccaaaga ccagtgtttt tccccgccga ccaaaaaaaa
     9361 gcggcttgcc tggtttgaat tttattttcc aaaatatttc ctctacaccc atttccttta
     9421 gaagcggctt ggcaaaatcc ttgtcaccaa ctgacattcc tccggaaata agtgctacat
     9481 ctgtctgaag tgcttcttca aatgcctgta tcaagatttc cggaatatcc ggaaccagcg
     9541 aaatttttac cagttcgcaa gactcctgcc ttaccatgcc tgccagcatg aagcggtttg
     9601 actcccggat ttgtccggct tgcggcttcc cctcaacgtc aaccaattcg cttcctgtgg
     9661 aaacaatgct tatttttggt aggcggtgaa ccggaacctg tgcataacca aaagtcgcca
     9721 gcacggcgat ttgtgccggc ccaatcaagg ttcctgccgg aatcattatt cgaccctccg
     9781 gaatgtcttc accctgatta cgcacgtttt gcgttttcgg gatcgcttga gtgaagtata
     9841 caaaggactc attgagttcg atatcttcct tcatcacaac cgtatcagct ccaggcggaa
     9901 gtggcgctcc tgtagcgatt tgcaggcatt gtccggaatt catctgctcc tctccggtat
     9961 cacctgcagc aatgtaaccc agttttttca ggcgcaccgg atttttgcgg gaggcatcta
    10021 ccaaatccgc cgactgaaca gcataaccat ccacggcaga attatcgaaa agcggcagtc
    10081 cgtggggtgc taaaatgtct tctgaaagag ccagaccctg agattcagtc agtggacaat
    10141 tcctttttcc taaaggcttt acatgttcga aaaccaggct tctggcgtct tcataagata
    10201 acatcacgtt ttacatgttt ttttaatgtc gttttgtttc cagataaaac ccaatttctc
    10261 tttgtcctaa ccctgaagta ctccattcat gaaaacccag cttcgtcaaa ttttcaagca
    10321 gattcaacca ttcgatgtga tcccacgtct ggtgtactct gagccaggcg tttagttctt
    10381 ttttgggaaa acggttttta atttttgcag gcatgattat tcttttattt ttagcttatg
    10441 ttttggtgaa aaattttata gaccactacc cattttacgc atttttcaca aactcaaaag
    10501 tcccttgtgc attttttttg tgagttgcag aatcatgcag gaaaacttga aacagttatg
    10561 atgaaatgtt agtatgatgg atcattttag aggagattga ttatgtatat ttttgcttta
    10621 acactgcacg taattgcggt gatgctggta atcggcactc tgtttgtgca gtctttgtcg
    10681 gttgtttttc gcttacgttt aagtaatcag gtgcagatcg aggggattca gtttgtacag
    10741 cgtcgtgttc atcgactgat ttattaccca gttctggctg tggcagttgc ttccggggcc
    10801 tatctggccg tgattcagga acggatcagt agtcctggca acggctggct gcacacaaag
    10861 atcacacttt tactgatctt gattattttg ggttttgtca acagacgtca aattatgaac
    10921 attgatttgc gcaagcctca ggccttgttg gttcacattg gtattttttt cgtttcagca
    10981 gcaatgattt ttctggcaca ggcaaaaccg ttctgaattc tgtctcaatt cctacccgga
    11041 gaggtgtccg agtggtttaa ggtgatagac tcgaaatcta ttgtacgttc tgcgtaccgg
    11101 gggttcgaat ccctccctct ccgcca
//
LOCUS       DTVD01000048           11081 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1436_length_11081_cov_288.556594, whole genome
            shotgun sequence.
ACCESSION   DTVD01000048 DTVD01000000
VERSION     DTVD01000048.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1436_length_11081_cov_288.556594"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(128..760)
                     /locus_tag="EYO68_01800"
     CDS             complement(128..760)
                     /locus_tag="EYO68_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HIC06568.1"
                     /translation="MFTGIVQGVRKVTVITDLKGGRRLRIQLNDLAENLQHGSSVAVN
                     GVCLTAIKIEEGCAEFDIIQESLDRSNLGALVNGDSVDIERACYFGDEVGGHQVTGHI
                     DCTGKIDEIRKTPNNRDVVVYCEEEWGGYLIPKGWIAIDGVSLTVVDVGDNWFSISLI
                     PETLEHTVLGQKVEGDKVNLEFDLTTKVIVKTIQRMFPEIKKQILSGMKN"
     gene            complement(794..1177)
                     /locus_tag="EYO68_01805"
     CDS             complement(794..1177)
                     /locus_tag="EYO68_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIC06569.1"
                     /translation="MWWILSLLLLIVPSLEIWVLMQLTYLMPLSTIFLQCIVTLAAGV
                     WLMQGENFSLWTLVESELRNRRVPAEEVLADLLLWVGGVLLVVPGFLTDALGLVIFVP
                     AVRQESIRWLRNSMLKTMGRPPLES"
     gene            complement(1187..1600)
                     /locus_tag="EYO68_01810"
     CDS             complement(1187..1600)
                     /locus_tag="EYO68_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIC06570.1"
                     /translation="MTAEEIQNFLQQEFPENPLIVESCENRRARLRLEVDQTHLRPGG
                     TISGPTMMLLADTAVYAAILTTIGAVALAVTTNLNINFLRKPEPNLDLIGESWLMKVG
                     KRLIVGEVKIYSENDPEPVAHATCTYSLPTKSLPS"
     gene            1731..3755
                     /locus_tag="EYO68_01815"
     CDS             1731..3755
                     /locus_tag="EYO68_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HIC06571.1"
                     /translation="MNSLSFLNSQQKEAVTTTQGPVLILAGAGSGKTQVIIQRIAYLI
                     RKKQVPPQQILAVTFTNKAAEEMRERLKETLSGKQNGVHLSTFHALGVNMLRKSIHHL
                     GYRPNFIIYDKNDQLSVIKTIMEDQDFDDTGLIDAKSVHFEISQSKSTGEGPEVFLDQ
                     QESQQKQMIGKIYRQYQKTMKGCNAIDFDDILNLTLSLFEKHPEVMDNLTERFRYIMV
                     DEYQDTNRIQYKLLRHLTKQHRNLCVVGDDDQSIYGWRGAEVRNIFDFEQDYPEVKYV
                     RLEQNYRSTQIILQAANQVISKNTQRMPKKLWSEKQGGVKLDWLQSENEAEEVEVVTR
                     RIRTKILQHGNRYLDFSILYRSNFQSRSLEEALREAKIPYRVVGGASFYDRQEIRDAL
                     AYLKVIHNPNDEVSLHRIINTPRRGIGKTALVQANTCCQEMHLPLFKVMLRARKYEKI
                     PKNAAFTMEMFAGIIINYQQRFANERFANVFKELLDEVGYIRFLETQSGEPKLRERRV
                     KNVLELLSGIRTYSDKNPEDSLRQYLERITLFTENDNDADSKANQVSIMTLHSAKGLE
                     FPFVFMIGMSEGLFPNQRSLDEGGVDEERRLCYVGITRAQQELTFSMAKSRKRFGEII
                     RQEPSRFLLNINPELLTVPIIGEASEEVKKERRQESRSAFFTQFKQIEAS"
     gene            3761..4396
                     /locus_tag="EYO68_01820"
     CDS             3761..4396
                     /locus_tag="EYO68_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIC06572.1"
                     /translation="MNYFVRKALSFGIVIFCIISFSGFALAQTLIVVLGDSLTEGFGV
                     AKEEAYPHLLEKELQRKGHSVKVINAGISGSTSASAPSRLRWYIKAHPDIVILALGGN
                     DGLRGLSVKHMKKNLSKTIELAQSEKILILLAGMQIPQNYGTEYTESFRNVFHELARQ
                     YQLQMIPFLLKEVGGVSSLNQADGIHPNPEGHQIIVRTVVEYLEPLLPTRQ"
     gene            complement(4472..5791)
                     /locus_tag="EYO68_01825"
     CDS             complement(4472..5791)
                     /locus_tag="EYO68_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06573.1"
                     /translation="MEKEKLIKRKGVISRGKRGISNKARKGGARAIKPIPNLNFVKIQ
                     RQVIQATEEVKKLRNFSFMMRMRKTILAADSTDKGTHIDASIIGIQIKSKAVSSLVSK
                     ISSDLTSSAARVKLVRTVMQDKRDFPLSLYRNLMIQAALTIYLGDITPATLQIAGQTY
                     RLYLEKIISTHKKGLLVVQSKQLKNVNLDVISVKDLIKMEEDEERGVDENEALVIQEI
                     KITLKLLEYTDSLDEDTRDSITMTMHLDELHDLSSKHRVKSLFAGSGSTESTKNKHML
                     IVRKTLAALEVIKRIPVLQPIGLKIIAKLQEIDSKLPLPYLIEARFHMQALRILTLRI
                     VMKEFSARPSLRPTFNKAIVAYHKALKRALLSNPKRTDIIVLSEFAQVSYYAYEQRKI
                     LNLANHGVLKILEMGKKAVDVLAPSNRVYIKQQKDILSALKKMKNLD"
     gene            5957..6142
                     /locus_tag="EYO68_01830"
     CDS             5957..6142
                     /locus_tag="EYO68_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06574.1"
                     /translation="MNQLDYVDICPVESVLFKWNLREFRMIYAFCSAKKRYEKTFVHI
                     INNTLMRTILEQLLFDK"
     gene            6408..7532
                     /gene="carA"
                     /locus_tag="EYO68_01835"
     CDS             6408..7532
                     /gene="carA"
                     /locus_tag="EYO68_01835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIC06575.1"
                     /translation="MPQTYFPVNMGGTTEGLLVLSNGRFFRGKLRGAPVEATGEVIFN
                     TSMTGYQEILTDPSYKGQIVTMTYPQIGNYGINPDDLESKTTHASALIVRELSHISSN
                     WRATQSLDEWLIEHRIPVLDGVDTRSLVKAVRSSGECSGLLVPSDSRHTLDELREEAL
                     DLPLMAGQNLARQVSTRESYFWPSEAEGKPLTVIAYDFGIKYNILRIMNRLGMSVTVV
                     PWNTSAEEVLEMNPEGILLSNGPGDPAAVSEAIDSVRNLLGKLPIFGICLGHQILSLA
                     LGCKTYKLECGHHGGNHPVMDLATNKIEITSHNHGFSVEENSLPEDVRVTHRNLNDQT
                     VEGIESLVHSAYSVQYHPEAAPGPRDASYLFGRFLEMLRG"
     gene            7617..8627
                     /gene="fliG"
                     /locus_tag="EYO68_01840"
     CDS             7617..8627
                     /gene="fliG"
                     /locus_tag="EYO68_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HIC06576.1"
                     /translation="MSVGKLTGPKKAAILLLALGEDGAADVMKNLEEAEIQQVGYYMS
                     RFTDVSPEELDIVLEEFYRNSVMADEGVNISSSPDFVKNALTKALGADRAKELSDNLG
                     AGEEEAGLEALRYAEPIMIANYIRTEHPQTIALILSYLKNAEQSSAVLRDLPESLQAD
                     ILYRMAVIESIPPGVISEMNEVLTEEMKTAGSMATSVGGVEPVAEILNSVDKATETRI
                     LSSIEETNPELAEQIRELMFTFEDMALIDAKQMQLVMKDVDQADMVLALKTASDAVKE
                     LIFGSMSSRAAEMVREDLENLGPAKLSDVEAAQQKIIKVVKKLEEAGTITIAGAGGGD
                     LV"
     gene            8818..10212
                     /gene="leuC"
                     /locus_tag="EYO68_01845"
     CDS             8818..10212
                     /gene="leuC"
                     /locus_tag="EYO68_01845"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIC06577.1"
                     /translation="MPKTMFEKIWNSHLVHEQDGNSIIYIDRHLVHEVTSPQAFEGLR
                     LAGRRVLHPEATFAVPDHNVPTENQDQPISDPISALQVETLRQNCREFGITLFDLGDE
                     RSGIVHVMGPEQGLTLPGSTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLQQK
                     KPKTMLVQVEGVLPETVTPKDIILAIIGKIGIAGGSGCVIEYAGETIRSMTMEGRMTL
                     CNMTIEAGARAGMVAPDEKTFEHLKNRPFAPRGDAFDLAVNSWKKLPSDPDAEFDLKV
                     NLNANDVLPQVTWGTNPGMVTDINGRVPDPEKIQNPMERKSVEQALKYMDLRANLPIQ
                     EISIDRVFIGSCTNSRIEDLRSVAEFVKGRQVNEHVSAMIVPGSGIIKRQAEEEGLDR
                     IFTDAGFDWRQPGCSMCLAMNDDVLKPGERCASTSNRNFEGRQGKGGRTHLVSPLMAA
                     AAAIAGHFTDPRTL"
     gene            10239..10868
                     /gene="leuD"
                     /locus_tag="EYO68_01850"
     CDS             10239..10868
                     /gene="leuD"
                     /locus_tag="EYO68_01850"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIC06578.1"
                     /translation="MEAFNTLSGITVPFDKINVDTDQIIPKQFLKKIERTGFGVHLFH
                     DWRYIDDEGTKSNSEFILNYPRYQGSQILLTGENFGCGSSREHAPWALQDYGFKCIIA
                     SSFADIFFNNCRKNGILAVELQAEEVQALFDEVEANEGCLLTVDLPQQTVSTPEGKKF
                     SFKVDSFAKNCLLEGLDDIGWTLQFEDKIQDYEQQARSKQPWLFLEGQS"
     gene            10884..>11081
                     /locus_tag="EYO68_01855"
     CDS             10884..>11081
                     /locus_tag="EYO68_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06579.1"
                     /translation="MSVLRFLTLILLFTTLLGACGRSELIRIRMLTPVNEDKKDHSIL
                     TETTESSTPIYTNTSSGGSLYF"
BASE COUNT         3360 a         2200 c         2319 g         3202 t
ORIGIN      
        1 gctttctgtc tcttctgatt cccttttcaa agaacttgcg ttcaactcct gcaaaacact
       61 taatgataag cttaataaaa tttattgtca atactattta ttatcagcag cattattatt
      121 tatagattca atttttcatt ccagacagta tttgtttttt gatttctgga aacattcttt
      181 ggattgtttt gacaattact tttgtagtga ggtcaaactc aaggtttact ttatcacctt
      241 ccaccttttg acccagaact gtgtgctcca aagtttctgg aattaatgaa attgaaaacc
      301 aattatctcc gacatcaact acggtcagac tgactccatc aattgcaatc cagcctttgg
      361 gaataaggta gcccccccac tcttcctcac agtacacaac aacatctcga ttattaggag
      421 tttttcgaat ttcatcaatt tttccggtac aatctatatg gcctgtaact tgatgtccac
      481 caacttcgtc tccaaaataa caagcccgct caatatcaac ggagtccccg tttaccaaag
      541 caccaagatt actacggtca agtgattcct gaataatgtc aaactcagca cagccttctt
      601 caattttaat tgcggtcaaa catacaccat tgaccgcaac gctcgaacca tgttgaagat
      661 tttctgctaa atcattcagt tgaattcgca aacgccgacc gccttttagg tcagtaatta
      721 ctgtgacctt ccggactcct tgtacaattc ctgtaaacat tgtttttctg aaaatgactt
      781 gttcaaatgt aaattaactt tctaaaggag gtcttcccat agttttcagc atactatttc
      841 ttaaccatcg aatgctttct tgtcttactg caggaacaaa tattaccagt ccaagtgcat
      901 ctgttaaaaa ccccggtaca accagtaata ctcctccaac ccagagcagt aaatctgcca
      961 gcacctcttc tgccgggacc cttctgttac gtaattcact ttcgaccaaa gtccacaaag
     1021 aaaaattttc accctgcatc aaccatactc cggcagccag tgtaacaata cattgtaaaa
     1081 aaattgtgct gagaggcatt agatatgtta gctgcatcag cacccaaatt tccaaacttg
     1141 gaacaatcaa caataaaaga ctaagaatcc accacataaa ataccgttat gatggaagtg
     1201 attttgtagg aagggaataa gtacaagtcg catgagcgac aggttccgga tcattttcag
     1261 aatagatttt aacctctcct acaattaaac gtttgccaac tttcatcagc cacgattctc
     1321 ctataaggtc aagattcggt tcgggtttac gcaaaaaatt aatattcaag tttgttgtta
     1381 ctgctaaagc cactgctcct attgtagtta ggattgcagc atagactgca gtatctgcca
     1441 gcagcatcat tgtcggtcca gaaatggttc ctcccgggcg caaatgagtt tgatcaactt
     1501 caagacgtaa tcttgcacgt cgattttcgc aggactcaac gatcagcgga ttttcaggaa
     1561 attcctgctg caaaaagttt tgaatctctt ctgcagtcat aagtatgttt tgttataaca
     1621 atttttagaa aaagattctg gtttgaaatg gcttgctgaa acaccttggt tcaatcaaca
     1681 atattctatc acaaattaat caataatttt atcgaacttt tctgcatttc atgaattcat
     1741 tgtcatttct gaactcacag caaaaggaag ccgtcacgac aacacagggt cctgtactga
     1801 ttcttgcagg tgcaggcagt ggaaaaacgc aagtcatcat acagcgtatt gcgtacttga
     1861 ttcgaaaaaa acaggttccc ccccagcaaa tacttgcggt tacttttacc aacaaagcag
     1921 ctgaagaaat gcgtgaacgt ttaaaggaaa cactctccgg gaaacaaaat ggcgtgcatt
     1981 taagtacttt ccatgcactg ggagtcaata tgctgcggaa atcgattcat cacctgggtt
     2041 accgtccaaa ttttattatt tacgacaaaa acgaccaatt atctgtcatc aaaaccatca
     2101 tggaagatca ggattttgat gatacaggat tgatcgacgc aaagtcagta cactttgaaa
     2161 tttcccagtc aaagtcaaca ggggaaggtc cggaagtatt tcttgatcag caggaatcac
     2221 aacaaaaaca aatgattgga aaaatttatc gtcaatatca aaagactatg aagggctgta
     2281 acgccattga ttttgatgat atccttaacc tcacgttgag tctttttgag aagcatccgg
     2341 aggtcatgga caatttaacg gaacgtttcc gttatataat ggtagacgaa tatcaggaca
     2401 caaatcggat tcagtataaa cttttaaggc atctcacaaa acagcacagg aatctttgtg
     2461 tggtgggtga tgatgatcag tctatttacg gttggcgagg ggctgaagtt cgtaatattt
     2521 tcgattttga gcaggactat ccggaagtaa aatatgttcg cctcgaacaa aattatcgtt
     2581 caacccaaat tattttacaa gccgccaacc aggtgatttc taaaaatact cagcgaatgc
     2641 caaaaaaact ctggtccgaa aaacagggtg gggtaaaact tgactggctt cagtcagaaa
     2701 atgaagcaga agaagtagaa gttgtgacca ggcgaattcg cacaaaaatt ttacagcatg
     2761 gcaaccgata tttagacttc tcaattcttt accgttcaaa ttttcagtcc cgttcattag
     2821 aagaagcact acgtgaagca aaaattccgt atcgtgtggt tggcggcgcc agcttttatg
     2881 accgtcagga aatacgtgat gcactggctt atcttaaagt aattcataac ccaaatgatg
     2941 aagttagttt acatcgaatt ataaacacac cacggcgtgg aattggtaaa acagcacttg
     3001 tacaggctaa tacgtgttgt caggaaatgc accttcctct gttcaaggtg atgctgcgtg
     3061 ccagaaaata tgagaaaatt ccaaaaaatg cggcattcac tatggaaatg tttgccggta
     3121 tcatcatcaa ttaccagcag cgttttgcaa acgaacggtt tgctaatgta ttcaaagagt
     3181 tgcttgatga ggtcggttac attagatttc ttgaaactca aagcggagaa cccaaattgc
     3241 gggagcgccg tgtgaagaat gttctggaat tattgagcgg tatccggact tattctgata
     3301 aaaatcctga agatagtctt cggcaatatt tagaacggat aacactattt actgaaaacg
     3361 ataacgatgc agacagcaag gccaatcaag tgtcgattat gacactccac agcgcaaaag
     3421 gtctggaatt tccttttgtg ttcatgattg gcatgtcaga aggtttgttc ccaaaccagc
     3481 gttcactcga tgaaggcgga gtagacgagg agcggcgttt gtgctacgtc ggcattacac
     3541 gggcccagca ggaacttact ttttcgatgg ctaaatctcg gaaacgtttt ggagaaatta
     3601 tccgtcagga accatcaaga ttccttctca atatcaaccc ggaactgttg acagttccaa
     3661 ttattgggga agcttctgaa gaagtaaaaa aagagcggcg tcaggaatcc cgttccgctt
     3721 tttttacaca gtttaaacaa atcgaagcaa gctagattat atgaattatt ttgtacgaaa
     3781 agctttgagt tttgggatag ttattttttg cataatttcc ttttcaggat ttgcacttgc
     3841 tcaaactttg atagttgtac ttggagattc attaaccgaa ggttttggag ttgcaaaaga
     3901 ggaagcctat ccgcatttac tggaaaagga actgcagcgc aaagggcatt ctgtgaaagt
     3961 gataaatgcc ggcatcagcg gttctacgag tgcaagcgca ccttcccgtc tccgctggta
     4021 cataaaggct catccagaca tagttattct ggcacttgga ggaaatgacg gtttacgcgg
     4081 cttgagtgtg aagcacatga agaaaaattt aagcaaaaca attgagttgg cacagtctga
     4141 gaaaatcctg attttattgg caggaatgca aattccacaa aactacggta cggaatacac
     4201 ggaatctttc cggaatgtat ttcatgaatt agccaggcag taccaactgc agatgatacc
     4261 ctttctgctc aaagaagtag gaggggtttc cagtttgaat caggctgacg gcatccaccc
     4321 aaatcctgaa ggccatcaaa tcatagtccg gactgtagtg gaatatttgg aaccgctttt
     4381 gccaaccagg caataaaagt gattgttagg ggttaaaaac tttttcttca gacatgccat
     4441 gaggcgcagg ctgacttcaa tttagcagct ttcagtcaag atttttcatt ttctttagcg
     4501 ccgaaaggat gtccttctgc tgtttgatat aaaccctgtt gctgggtgca agaacatcca
     4561 ctgctttctt tcccatttca agaattttca acacaccatg atttgccagg ttcagtattt
     4621 tgcgctgttc atacgcataa tatgaaactt gagcaaattc tgacagcaca atgatgtctg
     4681 tacgtttagg attagataac aaggctcttt ttaaagcttt gtggtaagcg acaatcgctt
     4741 tgttgaaagt aggtctcaga gatggtcggg ctgagaattc tttcatcact atgcgtagag
     4801 taagaatccg caatgcctgc atatgaaatc tagcttctat aagataagga agaggaagct
     4861 tactatcaat ttcctgtagc tttgctataa tcttgaggcc gattggctgc agcaccggaa
     4921 ttcgtttaat tacctcaaga gcagccaggg ttttacggac gataagcatg tgtttgttct
     4981 tggtggactc agtacttccg gagccggcaa ataaactctt taccctgtgt tttgaggaca
     5041 gatcatgcaa ctcatccaaa tgcatagtca tagtaatgct gtcgcgcgtg tcttcatcaa
     5101 gagaatcagt atattccaac aattttagtg taattttgat ttcctggatt acaagcgcct
     5161 cattttcatc aaccccccgc tcttcatctt cttccatttt gatcaggtct ttaactgaga
     5221 taacatccaa atttacattc tttaactgtt tcgattgaac cacaagcaat ccttttttat
     5281 gtgtgctgat gattttttct aaatataaac ggtatgtttg cccggcaatt tgcagagtcg
     5341 ccggtgtgat gtcacccaaa taaatagtga gagctgcctg tatcatgagg ttgcggtaaa
     5401 gcgacaaagg gaaatcccgc ttgtcctgca taactgttct gactaatttt acccttgctg
     5461 cacttgaagt gaggtcactg ctaatttttg aaaccaggga agaaactgcc ttacttttta
     5521 tctgaattcc gattatgctt gcgtcaatat gtgttccttt atcagtagaa tctgcggcaa
     5581 gaatcgtttt gcgcatcctc atcataaagg aaaagtttct caactttttt acttcttcag
     5641 ttgcctgaat tacttgcctt tgaattttaa caaaattgag gtttggaatc ggtttgattg
     5701 cacgagcccc tcccttcctg gctttattac taatccctct tttcccccgg gaaatgacac
     5761 cctttctttt tattaatttt tccttttcca ttactaataa cgtcttatta caattcagtt
     5821 attgttatta aaacatggct tttttaggtt aattgaaaaa aaccgacaaa gtcttcgatg
     5881 ttttatttct agcatatttt aggcaataac taaagtttgg gctgtttatt aataaaaaac
     5941 agtgataaca gatttaatga atcaactgga ctatgtggac atttgccccg ttgagtcagt
     6001 actttttaag tggaatctaa gagaatttcg catgatttat gcattttgca gtgctaaaaa
     6061 aagatatgag aaaacctttg ttcatattat aaataatact ctgatgagaa caattttaga
     6121 acaattatta tttgacaagt aaagaatatg ttgtggaaat tacaaaggaa gcactagacc
     6181 tttcaatata gagggagtat aattttcagc aagtgagatc acttcagata aattgctttc
     6241 cagcaaaaat atacaaaaaa aaatttaggt aatactcaca ttggaatttc cggaaaaacg
     6301 gaattttaat gtacattaat ccgaaccatc cgtgtagaga tttcaaatac tactggctat
     6361 aaatttcctt tgaccaaaac tctacaatat atatatttaa ggcttccatg ccacagacct
     6421 attttcctgt aaatatggga ggtacgactg aaggactgct tgttttaagt aatggacgat
     6481 tttttcgtgg aaaattgagg ggtgccccgg ttgaggctac aggtgaagta attttcaaca
     6541 cgtcaatgac cggttatcaa gaaattctga ctgacccgtc ttacaaaggt caaatagtga
     6601 caatgacata tccacagatt ggcaactatg gaataaatcc ggatgacttg gagtcaaaaa
     6661 caacgcatgc ctcagcactg atagtacgtg aactaagtca catcagcagc aactggaggg
     6721 ctactcaatc attagatgag tggttgatag aacacaggat tcccgttttg gatggagtag
     6781 acacccgctc tttggtgaaa gctgttcgtt caagcggaga gtgttccgga ctacttgttc
     6841 catcagattc acgacacact ctggatgagc ttcgtgaaga agcgctcgac ctgcctttga
     6901 tggcaggtca aaatctggca cgacaagttt cgacccgtga atcatatttt tggccgtctg
     6961 aagcagaggg aaaaccgctg actgttattg cttatgattt tgggataaag tacaatattt
     7021 tgcgaataat gaacagactg ggaatgagtg tgacagttgt accctggaat acatcagcag
     7081 aagaggtgct tgaaatgaat cctgaaggta ttttactgtc caatggccct ggagatcctg
     7141 ctgcggtctc ggaagcaatt gattctgttc gaaatttgct cggtaagttg ccaatttttg
     7201 gaatctgtct gggacatcag atattatcat tggccttggg ttgcaagact tataaactag
     7261 aatgcggaca tcatggtgga aatcaccctg tgatggattt agcgaccaat aagattgaaa
     7321 tcaccagcca taatcatggt ttttctgtag aggaaaattc cctcccagaa gatgtccggg
     7381 taacgcaccg caacctgaat gatcaaactg tagagggtat tgaatcactt gttcactcag
     7441 cttattctgt acaatatcat ccggaggcag ctcctggacc aagagatgca tcttacctgt
     7501 ttggccggtt tcttgaaatg ctgagaggtt gactattaaa ttccaacttc aaaaagtaat
     7561 ttgaagtaaa tcaaaatcaa aaaatcatta atcagattaa aagcaagtga ggctagatga
     7621 gcgtaggtaa attgacaggc cctaaaaagg ccgcaattct attgcttgcc cttggcgaag
     7681 atggagcagc agatgtgatg aaaaatttgg aagaagcaga aatccagcag gttggttatt
     7741 atatgagccg ctttacagac gtttctcctg aagaattgga tattgttctt gaggaattct
     7801 acagaaattc ggttatggct gatgaaggtg tgaacattag ctcatcgcca gattttgtca
     7861 aaaatgcact gaccaaagcc ctgggggcag acagggcaaa agagctaagt gacaatcttg
     7921 gtgcgggtga agaagaggca ggtcttgaag ccctgcgcta tgcggaaccc atcatgattg
     7981 caaattatat ccgcactgaa caccctcaaa cgatagccct tattctttct tatttaaaaa
     8041 atgccgaaca gtcttctgcc gtgctgcgag acttgcctga aagtttacag gctgatatat
     8101 tgtatcgaat ggcggtgatt gaaagtattc ctccgggagt aatttcggaa atgaatgagg
     8161 ttctaactga agaaatgaaa acagcaggta gtatggcaac aagtgtgggt ggtgttgaac
     8221 cagtggcaga aatcttaaac tctgtggaca aggcaacaga aacccggatt ctttccagta
     8281 tagaagaaac aaatcctgaa ctggcggaac aaatccggga attgatgttc accttcgaag
     8341 atatggctct cattgatgct aagcaaatgc agctcgttat gaaagatgtt gatcaggctg
     8401 acatggtgct cgcgctcaaa acagcaagcg atgctgttaa agaacttatt ttcggcagca
     8461 tgtcatccag agctgctgaa atggtacggg aagacctgga aaaccttggt cccgccaaat
     8521 tatctgatgt ggaagctgca cagcaaaaaa tcatcaaagt tgtcaagaaa cttgaggaag
     8581 ctggaacgat taccattgct ggtgcaggtg gtggagacct agtttgatta aactttgatg
     8641 cgaactgctg gttagttgta gtgaataaat cataatgctg tgttttgcaa attaattgaa
     8701 attaatggtt tgtggatttt caagttgttt cctaccttat aatcatgagg ttttttagct
     8761 gaagcctttg tttcttaaag taaactttca aaaaataatt aaagactgag taaaataatg
     8821 cccaaaacaa tgtttgaaaa aatctggaat tcacatcttg tacatgagca ggatggaaac
     8881 tcaatcattt atattgatcg tcacctggtg catgaagtga cttctccaca agcgtttgaa
     8941 gggttacgtc ttgcaggacg ccgagtgttg catccagaag caacatttgc agttcctgat
     9001 cacaatgttc caaccgaaaa tcaggatcag ccgatttcag atccaatttc tgcattacaa
     9061 gttgagactt tacgtcagaa ctgtagagaa tttggaatta ccctttttga tttgggagat
     9121 gagcgttcag gaattgtaca tgtaatggga ccagaacaag gtttgactct acccgggtca
     9181 actatagttt gcggagattc acacactgca acacatggcg cttttggtgc acttgcattt
     9241 gggattggca cctctgaagt tgaacatgta cttgccactc aaacactgca gcagaaaaaa
     9301 cccaaaacaa tgctggttca agtcgagggt gtacttccgg aaactgtcac acctaaagat
     9361 attatcctgg caatcatcgg aaaaatcggt attgccggtg gatcaggttg cgtaattgaa
     9421 tatgctggag aaacaatacg ctccatgact atggaaggtc gtatgactct ctgtaacatg
     9481 acaattgaag ccggtgcacg agcgggaatg gtagcaccgg atgaaaaaac ttttgaacac
     9541 ctgaaaaatc gaccttttgc accacggggt gatgcatttg atttggctgt gaattcgtgg
     9601 aaaaaactgc cgtctgatcc tgatgcagaa tttgacctga aagtaaacct aaatgcaaat
     9661 gatgttttac cgcaggtaac atggggaacc aatccgggaa tggtgactga cattaacggt
     9721 agagtcccgg accctgaaaa aattcaaaat ccgatggaac gcaagtcggt tgaacaagct
     9781 ttgaaatata tggatctcag ggcaaatctt cccattcagg aaatcagtat tgatcgtgtg
     9841 tttattggtt catgtacaaa ttcccgtata gaagatttgc gttctgttgc agaatttgtc
     9901 aaaggccgcc aagtaaatga acacgtttct gcaatgatag ttcctggaag cggaattata
     9961 aaacggcagg cagaggaaga aggccttgat agaatattta ctgatgcagg atttgactgg
    10021 cgtcagccgg gatgttccat gtgtttagcc atgaacgatg atgtgctcaa accaggtgaa
    10081 cgttgtgcct cgacaagtaa ccgaaatttt gaagggcgcc aggggaaggg tggccgtaca
    10141 catcttgtaa gtccactgat ggcagcagca gcagcaattg ccggacattt tactgacccg
    10201 agaactttgt aaattcaacc ttgacttaaa cttaaataat ggaagcattt aacacacttt
    10261 ccggaatcac tgtaccattt gataaaataa atgtggatac agaccaaatc attcccaagc
    10321 aatttctgaa aaaaatagaa cgtacaggtt ttggcgttca tctttttcat gactggcgct
    10381 acatcgacga tgaagggact aagtccaatt cagaatttat tctgaattat ccgcgttatc
    10441 aagggtcaca aattttgctg actggtgaaa attttggctg cggctcaagc cgtgaacatg
    10501 ctccatgggc tttgcaggat tacggtttta agtgcataat tgcgtccagt tttgcagaca
    10561 tttttttcaa caattgcagg aaaaacggaa tcttggcagt agagcttcaa gcagaggaag
    10621 tacaggcctt gtttgatgag gttgaagcca acgaaggttg tctgctgaca gtcgatcttc
    10681 cccagcagac tgtaagtact cctgaaggca aaaagttctc tttcaaggtt gattcatttg
    10741 caaaaaactg tctactcgaa ggtttggacg atattggctg gaccttgcag tttgaagaca
    10801 aaattcaaga ttacgaacag caagccagga gcaaacaacc ttggttgttt cttgaggggc
    10861 agtcttagcc agttcaggaa attatgtcag tcttacgctt cttaaccctg attcttttat
    10921 tcacaactct tcttggagct tgtgggcgtt cggagttgat tcgaatacgc atgctcaccc
    10981 cagtaaacga agacaaaaaa gatcactcta tcttaactga aacaacagaa tcttctacac
    11041 ctatatatac caacacatca agcggcggta gtttatattt t
//
LOCUS       DTVD01000049            2826 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14487_length_2826_cov_343.626850, whole genome
            shotgun sequence.
ACCESSION   DTVD01000049 DTVD01000000
VERSION     DTVD01000049.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14487_length_2826_cov_343.626850"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            165..1451
                     /gene="hisD"
                     /locus_tag="EYO68_01860"
     CDS             165..1451
                     /gene="hisD"
                     /locus_tag="EYO68_01860"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIC06580.1"
                     /translation="MANWIKEAISEEQRDEAQKQIRDTVEKLLEDIDKRGDTAVRELS
                     KRFDNWSPDEFRLSEKEIQSCIDRLDAQTIGDISFAQEQIRNFAQIQRDSMKDVEEET
                     LPGVILGHRNIPTNRVGSYVPGGKFPMVASAHMSVVTAKVAGVKEIITCAPPYEGRPS
                     DAIVAAQHLGGADDIFVIGGVQAVAAMALGTESIPAVDMLVGPGNAYVAEAKRQLYGR
                     VGIDLFAGPTETLVIADETVDGEMCATDLLGQAEHGPTSPAVLLTNSMNLARQTLEEV
                     KKQMKTLPTSDTAAPAWKNYGQIIVADSYDEMLEIANELAFEHVQVMTKKDDWFLENM
                     INYGALFLGPRTNVSYGDKVIGTNHTLPTLKAARYTGGLWVGKFLKTCTYQKILNNEA
                     SVMVGEYCSRLCKLEGFAGHQEQADLRVRRYSGEAA"
     gene            1448..2203
                     /locus_tag="EYO68_01865"
     CDS             1448..2203
                     /locus_tag="EYO68_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC06581.1"
                     /translation="MTAAKVYDLRGQRALVTGAGRGIGEACAKTLAAAGAEVVLTART
                     EKQLEQVRDEIIKSGGKASVHAADIYSMEAVNNLKKLGPFNILVNNAGNNIPEHFCEV
                     TEERFDKVMDLNVKSAFFVAQVVARGMVESGIRGSIIHISSQMGIVGGRNRTVYCASK
                     HAMEGFCKSMALDLAENGIRVNTVCPTFIETDLTRPFMAEKEFKDYILNRIPLGHVGQ
                     TEDVADAVLFLASDMSKMVTGSTIKVDGGWTAV"
     gene            2247..>2826
                     /locus_tag="EYO68_01870"
     CDS             2247..>2826
                     /locus_tag="EYO68_01870"
                     /inference="COORDINATES: protein motif:HMM:PF03737.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIC06582.1"
                     /translation="MKQIPESLLNTFRKYDTPTIVNSLELLDSKFRTSCFTTEQMICA
                     DTTLPPIVGYARTATISASSEVDPNTKRDRSLAYYEYVSSGTEPFISVIQDIDESPGF
                     GAFWGEVNSNIHNALGVIGVVTNGSVRDLDVLSAGFQVLAGKIGPAHAYVRIEETGIP
                     VNIFGMNVQHNDLIHADRHGSVVIPAELAEELP"
BASE COUNT          849 a          591 c          698 g          688 t
ORIGIN      
        1 ccatgaccca tgctgaaatg caggaaggtc ttgtagtcag ggaatatcat gcgattgatg
       61 aggtctcact ttggatgcaa atcagcagct attctatgag ttaagacatc gaaaaaccgg
      121 ttaaaaatta agtacaaaaa tcattcaata aaataaggag aattatggca aactggataa
      181 aggaagcaat ttcagaagaa caacgggacg aagcccagaa acaaatacgt gatactgtag
      241 aaaaattgct tgaggatatt gacaaacgag gagacacagc cgtcagggaa ctttcgaaac
      301 gttttgacaa ctggtcaccg gatgaattca ggctttctga aaaagaaatc caatcctgta
      361 ttgatagact tgatgcacaa acaattggtg acatcagctt tgcacaggag cagatcagga
      421 acttcgccca gattcagcgg gattccatga aagacgttga agaggaaacg cttccgggag
      481 tgattcttgg tcacaggaat attcccacca accgtgtggg gagttatgta ccaggaggta
      541 agttcccaat ggtagcctct gctcacatgt cagttgtaac tgcaaaagtt gcaggcgtga
      601 aggaaatcat tacctgcgct cccccttatg agggacgccc atctgatgcc atcgttgcag
      661 ctcagcatct tggaggagca gacgatattt tcgtgatcgg aggtgtccag gcagtcgcgg
      721 ccatggcatt aggaactgaa tccatacctg ctgtggacat gctcgttggt ccgggcaatg
      781 cctatgtagc agaagctaag aggcagctgt atggacgagt cggtattgat ctttttgccg
      841 gaccaaccga aactcttgtc atagcagatg aaacagttga cggagaaatg tgcgctacag
      901 acctgctggg ccaggccgaa cacggtccga cttctcctgc tgtcctttta acaaattcca
      961 tgaatcttgc caggcagaca ttggaggaag taaagaaaca gatgaaaact cttccaacca
     1021 gtgacacggc agcgcccgca tggaaaaact acggacagat tattgtagca gactcttatg
     1081 atgaaatgct tgagattgca aatgagctcg ccttcgaaca tgttcaggta atgaccaaaa
     1141 aagatgactg gtttctggaa aacatgataa attacggcgc acttttcctt ggacctcgta
     1201 ccaatgtttc atatggagac aaggtcatag gtactaacca tactttgcct acattaaaag
     1261 ccgcacgata caccggaggt ctctgggttg ggaaatttct caaaacatgc acctaccaga
     1321 aaattctcaa caacgaagcc agtgtaatgg tgggagaata ctgttcaaga ttatgcaagc
     1381 ttgaaggatt tgccggacac caggaacagg cagatctcag ggttagacga tatagtggag
     1441 aagctgcgtg acagccgcaa aagtttacga tttacgggga cagcgggctc tggttacagg
     1501 cgcaggcaga ggtatcggtg aagcttgtgc caaaaccctg gccgctgcgg gcgcggaagt
     1561 tgtgctgact gccaggacag aaaaacagct ggaacaggtc agggatgaaa tcattaagtc
     1621 cggaggaaaa gcctcagttc atgcggccga tatttactca atggaagctg ttaataattt
     1681 gaaaaagtta ggtcctttta acattcttgt caacaacgca ggcaacaaca ttccggaaca
     1741 cttctgtgaa gtcactgaag agaggttcga caaagtcatg gaccttaatg tcaaatcagc
     1801 cttttttgtt gcccaggttg tggcaagagg tatggttgag tctggaatca gaggatccat
     1861 aatccacatc tcaagtcaga tgggcatcgt tggaggacga aaccggaccg tctactgtgc
     1921 atccaaacat gcaatggaag gtttttgcaa aagcatggct ttggatttgg cagaaaatgg
     1981 aatcagggtg aacacggtat gcccaacttt cattgagacg gatttgacaa ggccctttat
     2041 ggcagaaaag gagttcaaag attatatttt aaatcgaatc ccgcttggac acgttggaca
     2101 gacagaggat gtggcagacg cggttttgtt tcttgcctct gacatgagta aaatggtgac
     2161 cggttctacc atcaaggttg atggaggctg gactgcagta taatcccaac aagtcaaatt
     2221 acttaaactc taaaaaatga ttgccgatga aacaaatccc ggaaagcctg cttaatactt
     2281 tcaggaaata tgacacacct acaatcgtga attcgcttga actcctcgat tctaaattcc
     2341 gtaccagctg ttttacaact gaacagatga tttgtgcgga taccactcta cctccgattg
     2401 tgggttatgc ccgaacagca acaatcagtg catcttctga agtggatcca aatacaaaac
     2461 gtgaccgaag tttagcttat tacgaatatg tttcttccgg aacagaacca ttcatcagcg
     2521 ttatccagga tattgatgaa agtccgggat ttggggcttt ctggggagag gttaattcaa
     2581 acattcataa tgcacttggg gttattggtg tggttaccaa tggctcggtt cgggaccttg
     2641 atgtcctttc agcaggattt caggtgctgg caggtaaaat tggaccagcc cacgcttatg
     2701 tgcgcataga agaaacaggc atacctgtaa acatttttgg aatgaatgtt cagcacaatg
     2761 atctgattca cgcagaccga catggctccg tagtcatacc agcagaactt gcggaagaac
     2821 ttccga
//
LOCUS       DTVD01000050            2824 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14504_length_2824_cov_315.802817, whole genome
            shotgun sequence.
ACCESSION   DTVD01000050 DTVD01000000
VERSION     DTVD01000050.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14504_length_2824_cov_315.802817"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..166)
                     /locus_tag="EYO68_01875"
                     /pseudo
     CDS             complement(1..166)
                     /locus_tag="EYO68_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            203..2323
                     /locus_tag="EYO68_01880"
     CDS             203..2323
                     /locus_tag="EYO68_01880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HIC06583.1"
                     /translation="MITVIIASAFFGLGVALFYYLKVSRIPLTQGIDNPEEASKLIKI
                     HGAIATGAMAFLKAEYKYMVYFMAGFAIVIALLIDDPHTPEVNEGIYTAISFLLGCVI
                     SIVSGFIGMRIATIGNARTTTAAKNSIAEAFKVALNSGAVMGFGLVGLGVLGLIAIYL
                     LLKFLLPEIEPHILMEVLAGFGLGGSSIALFGRVSGGIFTKAADVGADLVGKVEQGIP
                     EDDPRNPAVIADNVGDNVGDVAGMGADLFGSLAESTCAALVIGAVAFATNLEALLFPI
                     LISAVGIPASLLTMFSARVSEEKDVAPVLKRLLIISTVLSAIAVFFVTMWALPESFEI
                     GGQLYTNMGIYFCFLSGSIAGLLVGLLTEYYTSHDFKPVREVAKASETGAATNIIFGL
                     ALGYHSATGSILLLAASIYASFTLGGMYGVAVAALGMLSTLVVGLTIDAYGPVADNAG
                     GIAEMTGMGESVRDRTDVLDAAGNTTAAIGKGFAIGSAILTSLALFSAFLTRADLLDP
                     QAKIMDSINLLDPLVFTGLFVGAMLPFLFSAMTMKSVGKAAFDMIEEVRRQFRTIPGI
                     MEGTAEPDYEKCVSISTEAALREMIPPGILIMGTPLLVGFLFGVPAVAGILAGSLVSG
                     GVLAISSANSGGAWDNAKKYIEKGNLGGKGTETHKAAVVGDTVGDPLKDTSGPALNIL
                     IKLSAILSLVFVPFFIQYGGLLIG"
     gene            2405..>2824
                     /locus_tag="EYO68_01885"
     CDS             2405..>2824
                     /locus_tag="EYO68_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIC06584.1"
                     /translation="MSNSKIYLDHNATSPLLPEVENVMSEVAQQAFANPSSPHWAGRN
                     ARFLLEEARDSLANSLGVKSQEILFTSGGTESNNTVLRQLLLISGEQHLISSAVEHPS
                     VLETCRILAGQPNIQFTELVVDSSGKVEPEKLREVLRP"
BASE COUNT          693 a          611 c          668 g          852 t
ORIGIN      
        1 tcattattgt tttgaattta tttctacaaa ctaagtcaaa gacagtagca gcaatttgcc
       61 agttattttg ccgtttacac tttattttgt gtggaaagac atttagagca gaatgcctat
      121 tttttacatt gttcgcaagc gttctttttt tggggaaaga acaccggcga ttcctttttc
      181 agccccagac acaggagaat taatgatcac agtcatcata gcttcagcat tttttggact
      241 tggagtagca cttttttatt acctcaaagt ctccagaatt ccactcacac aggggatcga
      301 taatccggaa gaagcctcaa agcttattaa aattcacggt gctatcgcta caggtgccat
      361 ggcttttttg aaagcagagt acaagtacat ggtttacttc atggcagggt ttgcgatcgt
      421 cattgccctg ctgattgatg acccccatac tccggaagtc aatgaaggaa tatataccgc
      481 aatttcattt ttgctgggct gcgtcatttc gattgtttca ggctttatcg gcatgcggat
      541 tgctactatt ggaaatgcaa gaacaacaac tgctgccaaa aattcaattg cagaagcttt
      601 taaagttgcg ctgaattccg gtgcagtaat gggctttggt ctggtcggtc tgggagtact
      661 tggtctgatc gcaatttatc tgttgcttaa gttcctgttg ccggagattg aaccacacat
      721 tttgatggaa gtcctggcag gttttggtct gggcggttct tccattgcac ttttcggacg
      781 tgtaagcgga ggtatcttta ctaaagctgc agacgttggt gcagaccttg tcggcaaagt
      841 tgaacagggt attccggaag atgatccgcg gaatccggca gttattgcag ataacgttgg
      901 agacaatgtt ggtgatgttg ctggaatggg tgcagacttg tttggttcat tggcagaaag
      961 cacctgtgca gcactggtga ttggagccgt agcatttgcg acaaacctgg aagcactgct
     1021 ctttccgatt ctcatttccg ctgtcggtat tccggccagt ttattgacta tgttcagcgc
     1081 acgtgtcagc gaagaaaaag acgtggctcc agttttaaaa agactcttga tcatttccac
     1141 agttctgagt gcaatcgccg tcttttttgt cacgatgtgg gcactcccgg aatcttttga
     1201 aatcggcggt cagctttaca ccaacatggg tatttatttc tgtttcttga gcggttcaat
     1261 cgccggttta ctggtgggac tgctgactga atactacacc tcccacgatt ttaaaccggt
     1321 acgtgaagtt gccaaagcct ctgaaaccgg agcggcaaca aacattattt tcggactggc
     1381 ccttggatat cacagtgcta ccggttccat tttactgtta gcagcaagta tctacgcatc
     1441 cttcacacta ggtggaatgt acggcgttgc cgttgcagcg ttaggcatgc tgagtacttt
     1501 ggtcgtcgga ttgactatcg atgcttacgg tcctgttgct gataatgccg gagggattgc
     1561 cgaaatgacc ggcatgggtg aaagtgtccg ggaccgaact gatgtacttg atgctgcagg
     1621 aaatacaact gcagcaatcg gtaaaggatt tgccattggt tctgcaattc ttacttccct
     1681 cgcacttttt tctgcgtttt taacccgggc agatctttta gatccacagg ccaagatcat
     1741 ggacagcatt aacctgctcg atccgcttgt ctttaccgga ttatttgttg gagccatgct
     1801 gcccttcctc ttttccgcaa tgaccatgaa atcagtcggc aaggcagctt ttgacatgat
     1861 tgaagaagtt cgccgccagt ttcgtacaat tcctggaatc atggaaggta cagcagaacc
     1921 ggattacgaa aaatgtgtta gtatttctac agaagcggca ctgcgtgaaa tgattcctcc
     1981 gggaatattg atcatgggca caccgctttt ggtgggtttc ctgtttgggg ttcccgctgt
     2041 tgccggaatt ttggcaggtt ctctggtttc cggaggcgtt ctcgctattt cttcagcaaa
     2101 ttccggagga gcctgggaca atgccaagaa atacatagaa aaaggtaacc tgggtggaaa
     2161 aggtacagag actcacaaag ccgcagtggt tggagataca gttggcgatc ctttaaagga
     2221 cacttccggc cctgccctga acatccttat taaactttca gctattctca gcctggtttt
     2281 tgttccgttt ttcattcagt acggcggttt gttaattggc tgattacttg tggcggatct
     2341 gagggtacgc cactataaaa tttctattta agatcctctt tttgcatttc ttccagagtt
     2401 tccagtgtct aattccaaaa tctatttgga tcataatgca acttcaccat tattgcctga
     2461 ggtggaaaat gtgatgagcg aagttgctca gcaagcattt gccaaccctt ccagtccaca
     2521 ttgggctgga cgcaacgcaa gatttttgct tgaagaagca agagacagcc tggcaaattc
     2581 tttgggagta aaatctcagg aaattctttt caccagcggt ggaactgaaa gtaacaatac
     2641 tgtactccgg caattattat tgatttccgg agaacagcat ttaatctcct ctgccgttga
     2701 acatccttct gtacttgaaa cttgtagaat tctcgctggt caacctaaca ttcaattcac
     2761 agaactggtt gtcgattcat ccggaaaagt tgaaccggaa aaattgcgag aggtacttcg
     2821 tccg
//
LOCUS       DTVD01000051           33801 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_145_length_33801_cov_380.780359, whole genome
            shotgun sequence.
ACCESSION   DTVD01000051 DTVD01000000
VERSION     DTVD01000051.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 33801)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33801)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33801
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145_length_33801_cov_380.780359"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="EYO68_01890"
     CDS             complement(<1..254)
                     /locus_tag="EYO68_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC06585.1"
                     /translation="MSPNISCLIYDMDGLLLDTEGIYTEVTQQIVGEYGKVFDWSVKE
                     KIIGRRSIQAAEIIVESLDLPISPQDYLDSRKDVLLEKFK"
     gene            complement(488..1069)
                     /locus_tag="EYO68_01895"
     CDS             complement(488..1069)
                     /locus_tag="EYO68_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06586.1"
                     /translation="MGNGIYLEKKLLDSHSQRVILKIVNIKSTESHVYLFRDQNGVLG
                     LNIVKAEADRDARFWQIDPEFYQYSNNITGLKRIFRIFQNNIQPILPYLRTGSGVTSA
                     ATHVIFYHIADSKEVIRLNASGQEIKTRLYTFRLHVINRKLGDSKSIGNLLIQDLNYR
                     LKLVWEDENSVSYKLSNGEKYTVDLLKYVPELF"
     gene            1809..3767
                     /gene="selB"
                     /locus_tag="EYO68_01900"
     CDS             1809..3767
                     /gene="selB"
                     /locus_tag="EYO68_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIC06587.1"
                     /translation="MDSNKTAALSSRPFVIGTSGHIDHGKTTLVKALTGVDTDRWAEE
                     KKRGITIDLGFAHYEDEQGNQMAFVDVPGHEKFIHNMLAGVAGLDAVLLVVAADEGVM
                     PQTREHLNICNLLKIQTGLVVLTRIDLVEEEEMVELCREEVSELLKGTFLEGAPILAV
                     SPVTGEGLDELRKTLSKLPKQIQHPATEKPFRLHVDRSFTLKGFGTVVTGTVLSGTVR
                     QDEEILQYPQRRNVRIRGLQNHGQEVKEINAGQRAALNLAGIAKEEIQRGDQLAHPES
                     LLNSYLLNVSLTLLEDVLAPLKNRTRVRVHLGSKEVFGRITLLEGETLSPGKTALVQL
                     RLESLVSGSYGDRFIVRSYSPMITLGGGMVIDPSPGKSRRVQHELPERLERLHVGDEN
                     IRSEEVIYLQSVRGVTESEFSVRTGLSSRQSGKALQLLQSQQKVLCVEPLTGRYLHVE
                     HIVRIGKFLSKALEQHHKKFPDRDGMTRSELGGKLSLIFTEKEVEKLLKYLVKVGLFT
                     QNDQYISLAEHQPQASQSREDSLARCLELIKSGGFQPLRRTHLLQELGLNEKDGMTLL
                     KSAAHSKRLVRVAEDLHYIPEQIASIIESLRFYFSENPNITVIQFKELLNISRKHAID
                     LLEYFDSQQLTIREDNHRIPARITALNN"
     gene            3839..6181
                     /locus_tag="EYO68_01905"
     CDS             3839..6181
                     /locus_tag="EYO68_01905"
                     /inference="COORDINATES: protein motif:HMM:PF13458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06588.1"
                     /translation="MWNFQTVTVTAHAQSLLSDSEWLEENKDVFGKQELTLQNIPLRP
                     SDLSSTPTVLPESFLIPLETGGQYFSTVPQKPALPLIEQKDQSENPSGSFLSRLFPWF
                     SSTEQSTSTNLDTASEMHNAETRVEWADSEFAGIKMLITEIERLLISDPVAAREMYEE
                     IEDQLEIDERTRLKVQLLYYLKKWSSAEQLAEVFLSERPESKMASLMFYFLNKSLMAQ
                     HKPLSQNLIFRKRAVETLEAKFRSDFLFILSNEAFLEGDLLTAIQYRLEGMNKAKTAN
                     VASMEKLNGLLNQVQSPEILRILSENNKNVKWLQKKIFIMELELLAKLKRYRDALGIL
                     EQRMSAARVIGDQEELEVLKQIQIRYTNALNVNPRRIGVILPLSSSSVKVSRLAQQTM
                     NGLWLALHANEIPAVPENMGDDEASQENEITGKSEDKDNAQAESDSRKFEDSWELVVR
                     DSQLNPEITKSLVRELVETERVIAIIGPLARKTSEAAAEEAERLRVPLISLSLTESIP
                     ELGEFIFRNNQSWKQEIQELLEYATSELQACRFLILYAKTREGRQKMRLFWDAAVLKG
                     CKVVAVEGFKDEGQKSLVNEFDTFTGKIRRLGTEDKIILKELKEKEVPIHNFDAVFVA
                     VGSGGVKNLSLIFPYSEVYKMRKTTFLGDSGWNDSALPYAPGLRGVKNPVFVDSFFLQ
                     SKTPAMQQLLRLHERILYRHQNYIGPTAYTAFAYDTLIILMRLLEDERNQSHRDLRDA
                     LLNMQMFPGVTGNLRFDERGKVEREMQLLTLRRGKIQPLN"
     gene            complement(6241..6708)
                     /locus_tag="EYO68_01910"
     CDS             complement(6241..6708)
                     /locus_tag="EYO68_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06589.1"
                     /translation="MKWLKFETHEEEEVSDPENSCILVYLGPEALKHAVIVDDFGTFQ
                     DSPECIPGGRLLEVSRQYSYRLRQVADLVEEPLEEQKILMKAQSKRNFYAEIRPEKPL
                     EKKEDDLMIGGPNDSYPESAIGDAGNTGNTNEPQQNVKPEQVPFKDLESLFVK"
     gene            7019..7360
                     /locus_tag="EYO68_01915"
     CDS             7019..7360
                     /locus_tag="EYO68_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HIC06590.1"
                     /translation="MKTSASKNEILKRELEQLFGEQDANIETAAQCLKVMSHPVRLRI
                     LCALREGEQTVQKLEYYTGIKQSTLSQHLALLKSRGVLVSRRETTYSFYSFSNDQITQ
                     LFDLIKEVFCT"
     gene            7367..8425
                     /locus_tag="EYO68_01920"
     CDS             7367..8425
                     /locus_tag="EYO68_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC06591.1"
                     /translation="MTNSMAKPNQPKLVFRQFFDKDTGTFTYFLFDSETMEGLIIDPV
                     KEQFDLSLQFIEELGVELKYVIDTHVHADHVTSSCMLREATGAKSVFGEPAGIECADI
                     LLEDGDELEFGHFSLKAIATPGHTDACTSFYTEGMLFTGDSLLIRGCGRTDFQLGDPE
                     KLYESITQKLYSYPDDTLVYPGHDYLGRTASCIREEKAFNPRIGSGQTLEKFVQIMKN
                     LDLPKPKRIDSTVSQNLQCGFSLELGHVNEDNFTMHDLYQLLDNLKSTERVIDVRTPN
                     EYSQGHVPGSLNIPMGNEQSFLEELSSYQRIFVHCHSGRRAQTVYTMLGMQCLENVVC
                     INSSGMADWTIAGFPVER"
     gene            8440..9381
                     /locus_tag="EYO68_01925"
     CDS             8440..9381
                     /locus_tag="EYO68_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIC06592.1"
                     /translation="MKKSILVKLSLIPALLLIPTILWAASTEISGMPWWGWPTALFFT
                     TFFMGVFGVMAGIGGGVLFVPLVSGFFPFHIDFVRGAGLFIALGSSLAAAPHLLEKNL
                     ASLKLALPAALIASAFAIVGAFLGLWLSALNPAYIHGSLGLTIITIVFIMLFAKNTEN
                     PEVSRGNKLTELLQINGIYTEETTGKEVSWTVHHTKRGMFGFVVVGLLAGFFGLGAGW
                     ANVPILNLLMGAPVKVAVATSSFALSITDSTAAWIYMNKGAVLPIIVVPSMVGIMLGA
                     RIGSKLLPKVKPTVIRYIVITVLLLAGVRSLLKAFGI"
     gene            9401..9820
                     /locus_tag="EYO68_01930"
     CDS             9401..9820
                     /locus_tag="EYO68_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HIC06593.1"
                     /translation="MEKSIVFENAPEQHIYAKIIYHISNLGLLVLIMGFILYIFGVLT
                     PLVPLEELPKYWSLSLTQFLEKTGAPTGWRWTAMLGYGDVIPFLGVTILASVTFVCFL
                     ALLFSFLQRGAKVLAFIAAMELFFILISASNLIQISH"
     gene            complement(9933..11795)
                     /locus_tag="EYO68_01935"
     CDS             complement(9933..11795)
                     /locus_tag="EYO68_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06594.1"
                     /translation="MKSFDSNDKSFEERFDPKLRTIGDSQLQNHDQEKEQTPTSKFFR
                     LEFSESIPLETKTFLSEKLPDMLDFPGRLGLKINYASHLLRFIDQTTYETEIGSPLPK
                     NVTLPASRIDSFIDSRSFKVTIILPKKLETAEVIVNITRNVFSKLFGNIFFNEQILPL
                     EFYQQSVKGQKQITAAIPEILDLVKEFNFHSDSLEGHCESVAKSYRLSMEKEGDQIRK
                     QLIDEWREKWKAQSLSTEEQHTLSSIFTEFKELFRTNPEQFNQKVSERVKQLNSHLHF
                     ILPHEMRVYENFEQERFSHYIRSVLHKLEEISALTGFIEELYEQLKQPPEAVELDGVG
                     TQIRSRMRQLRKEKKVILFYVPEMPQNSELDNIRQKFPLRLIKMLPSGTPLKEWSKEI
                     KKMEKHYAESIYSKLYSALHSLSAWTQSPEESRAAGFLESADGLRLKRLMSVMKFRLP
                     AIKSIQSSLGVLLDTNEEAVLQAEPDTVPQRQLIPLDDFRKAWSYFCSSVLTLLYYQE
                     FTESAGLPQGFRADNYLKSILEFVDRQCSRGINHFHIVRLLWLIYEKKPEHDALTFIL
                     YCIQNPQDILRYTLHQVMRPQTDKTPVKRRLEKLPHYGEALITAYQNRLLEALV"
     gene            complement(11835..12074)
                     /locus_tag="EYO68_01940"
     CDS             complement(11835..12074)
                     /locus_tag="EYO68_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06595.1"
                     /translation="MSKVFYPFTEQPKTEQKSQNEDWQIEENYLNLWGYLRRRYPNVD
                     LEDKKFLDLIELKTTLEGPHKLREWNEDIASVRKH"
     gene            12492..13244
                     /locus_tag="EYO68_01945"
                     /pseudo
     CDS             12492..13244
                     /locus_tag="EYO68_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13522..14787
                     /locus_tag="EYO68_01950"
     CDS             13522..14787
                     /locus_tag="EYO68_01950"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003316840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIC06596.1"
                     /translation="MRDRIISIKAMEILDSRGNPTIRSTVILENGVTGTASVPSGAST
                     GENEALELRDGDKARYAGKGVLKAVENVNQEIAPELLGMTPDRQAEIDRMMLKLDGTA
                     TKSNLGANAILSVSMAVAVTAANSHNLPLYAYLGGVGGFRLPMPMMNIINGGEHADNS
                     VDLQEFMVMPIGAPTFREALRYGAESFHALKKILLKKGYATSVGDEGGFAPNLKSNEE
                     ACEVILEAIKAAGYEPGKDISLAMDAAASSFYKKGSYDFFKSGQGTKNSTEMLGFYSS
                     MVEKYPVVSIEDPMDEHDWDGFSAITKKLGKKVQIVGDDLFVTNTDFVKKGISKNAAN
                     AVLIKLNQIGTVSETVATIQLCQKAGWNYIISHRSGETEDTFMADFAVAMAGGQIKTG
                     SASRSERIAKYNRLLEIESELGKSAIFGS"
     gene            14842..16431
                     /gene="murJ"
                     /locus_tag="EYO68_01955"
     CDS             14842..16431
                     /gene="murJ"
                     /locus_tag="EYO68_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIC06597.1"
                     /translation="MTFIPLKNNPEESGRILKNARTVTIFTLFSRILGAARDLVIAHV
                     FGAGWVTDAFVQAFTIPNVLRRLTAEGSMTLAFLPLYTEIRERKDPEAAKKFAAKTLG
                     LVLAATTILTGLGILFSPQLVYLFAAGFASSPEKYDLTVLLTRVMFPYLIFVSLVAWA
                     MGVLNAEGRFAAPAAAPILLNIGIIGAAFGISPLLEEPMIGIGIGVLLGGISQILIQI
                     PSLRKVGQSFKPQNFLNDENIQRLLKLLGPSLLGVAVYQINIIVLRNLASFMPTGQVT
                     HYYNASRLSELTLGVFAFAITSAGFPELSQHTAAKNWEKIRNTLRFTMSTTLLVIFPA
                     SVGLAVVAEPIVSMLYLHGAYAWSDVQNTSLTLQAFALSIPAVAMIRLQTSVFFSLKD
                     TRTPVKVSLFSILLTGLLGWWWSQSLEIFGLALGLAAGTWFQWILLSLFLLKQSELRK
                     NWWPLRSSLLYMLASAGMGGFAWYYRGYGSWENGPFLVYNWVIFGGILLGSALLYGLL
                     LLVFREEQVLRLKNHMLQINT"
     gene            complement(16414..17685)
                     /locus_tag="EYO68_01960"
     CDS             complement(16414..17685)
                     /locus_tag="EYO68_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine
                     deacetylase/succinyl-diaminopimelate desuccinylase family
                     protein"
                     /protein_id="HIC06598.1"
                     /translation="MIESLFARIDSKREELVSFTQELVRIPTINPPGEDYLLCAELLG
                     NRLAKSGFSLLYERANNTPGDTERYPRHNVIARIEGKHSGPCVHFNGHLDVVVTGSGW
                     TVDPFEGLVRDGKIYGRGTCDMKGGLASAVIAMEAILEESIDFPGAIEISGTVDEETG
                     GYGGVAFLAEKGFFSKPRVDHVIIPEPLNVDRVCLGHRGAWWAEVETFGRIAHGSMPF
                     LGDSAIRHMSAFLELIESRLFPALDKKQTRMPVVPEGARRSTLNLQAVHGGEAESFEG
                     LPSPCVPDSCRLIMDRRFLIEEDITEVKAEVTDLLKELADSRPNFRYQIKDIMEFLPT
                     MTDADAPVVQAVSSAIQSILGKTPEHVISPGTYDQKHIARIGHLHDCIAYGPGILDLA
                     HQPDEYIVIDDMVTAAKVMAASTLELLGVNL"
     gene            complement(18174..19436)
                     /gene="kynU"
                     /locus_tag="EYO68_01965"
     CDS             complement(18174..19436)
                     /gene="kynU"
                     /locus_tag="EYO68_01965"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIC06599.1"
                     /translation="MPINISYLLESRTACEREDAADPLRSWQGAFYLPQGLIYLDGNS
                     LGPMPKKALEQLEQAIRKEWAEDLITSWNKAGWWELPETLGELIAPVVGAASGQVIVS
                     DSTSLNLFKTVHAALTLRPERTVMISEADSFPTDLYILEGVTSTHQNIQRRLLGKDGN
                     SLEELIDNQVAVVVFSHVNYRTGEILHIEELVQKTHDAGALFVLDTCHSAGVLPVELD
                     QWGVDFAVGCTYKYLNGGPGSPAFIYVAKRHQKEAVNPLSGWWGHARPFEFEENFHAA
                     EGIRRFRTGSQPVLSLRGLQAGLEIAQEVDLQQVRKKSQRLTQLFIELIESGCRNYGV
                     TLQSPIDHDKRGSQVTLHFENGFAVIQALIARKIIADFREPGSMRFGFAPLYLRYTDV
                     WDAAEALADVLKTEEWREERFRQKHEVT"
     gene            complement(19548..20186)
                     /locus_tag="EYO68_01970"
     CDS             complement(19548..20186)
                     /locus_tag="EYO68_01970"
                     /inference="COORDINATES: protein motif:HMM:PF01810.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIC06600.1"
                     /translation="MIDSIWGIFTIGLLLGAPSGIAPGPMLILIISETLRHGIRAGAK
                     VACIPLLTDIPVVLISGFLFAQISNMNILLGAISLFGSVFLLYLGTNSIRTTNAEITD
                     FTPRPLLLKELMIANLVNPNPYLFWFTVGAPLMVRSFQQTWGSGITFLFSFYLGLCGV
                     KLLLAIAAGKSRNFLHGILYRRIMQFLGFALIGFAIMLFRDGLIFLGILHQG"
     gene            complement(20183..21043)
                     /locus_tag="EYO68_01975"
     CDS             complement(20183..21043)
                     /locus_tag="EYO68_01975"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIC06601.1"
                     /translation="MEFLKYHAHGNDYLVSTSEHPDFSENELTSEQVRKICHRNFGLG
                     SDGILVGGKDDSGNCFTLKIYNPDGSTAEKSGNGLRIFARSLWDRKLVDQSKFKIVTS
                     GGIVCAQVDSSGQSVTVEMGSVSFQSNIIPVTGESREVLNETLEINGEKLTFCSATIG
                     NPHCVVLQRKISADETRRLGPLIETHPSFPNRTNVQFLEVLNRNTIRIEIWERGAGYT
                     LASGSSSCAAASVAHRLGLCDAEIAVLMPGGQIDITISENYNIIMKGSVTRIGKMTLD
                     LECLDFEIPA"
     gene            complement(21160..22779)
                     /locus_tag="EYO68_01980"
     CDS             complement(21160..22779)
                     /locus_tag="EYO68_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06602.1"
                     /translation="MNKSDITPISGDHFEDFMLLTNPDHKSTKTKQAWHALQQQTQTH
                     VPSTKSIDLNRICVDTIRMELLIFPVVEHWKRPLTNTEWNHIDKESVLLEKQMEKMRK
                     LSILVRKKDPLSFPDIAPLFNKLFKHNKELRNNWDEFKQAWSNYKKDWQIFQMKKSQV
                     RKSKHKSAKTLSVDSANNSSNEENITPPVSPPMFPRDRWHALRVGSKALEVEVLHQCQ
                     RFRNGTKQDPEEIKEATHPGVKFLVEELRKIYLKTHGFELIKRVKLVQERNDQLMQEL
                     QPYFPEPIISAKMLEKTKHSFKHRERSAAGSKNENKSAGKFLRKIILIILFIFLGTFL
                     IFFFQNQGQPPGKSLMKHLKFVPKNKTLTAKSSTANKLSEFNLEDMMQSFGSNIPVQE
                     SSERLKKMVTASPEILENIANNILREIGKNTVFVFSEAKSLFLFWKGKLEKFASPQDL
                     QKKLRQLDQQYKDVESLWREIGDNAPENVRLIPDFLDGKSTYALEMLDENGDPMQVYM
                     TEEGVLLRLSNGQLLDKPLTPTEAAQKIADY"
     gene            23053..23769
                     /gene="grxD"
                     /locus_tag="EYO68_01985"
     CDS             23053..23769
                     /gene="grxD"
                     /locus_tag="EYO68_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIC06603.1"
                     /translation="MKFKVVSPNVESSGNSGTDPKAQIEQMLSGSPVFLFMKGTPESP
                     QCGFSSKIADILKAWEVPYQSFNVLSDESIRQGVKDFANWQTIPQLYINKEFVGGSDV
                     VEEMSNNGELGDLLKEAFPGRDITPPPPPVEVQEVAALEAASILKENPEIRLLDVRTQ
                     HERETASLDNSVLLDQELVEEILGSWDQNTPLMFFCHMGERSRQAAQYFTSQGFQQVY
                     NISDGIKGWSSNVDSSIPQY"
     gene            23806..24195
                     /locus_tag="EYO68_01990"
     CDS             23806..24195
                     /locus_tag="EYO68_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HIC06604.1"
                     /translation="MKTEKSLPSVVLTERAAQVFKESCEAEGSTLEDTFLRIGARPGG
                     CSGWKYELESNTSEDIGPNDVQILSREVSVIVDREALTEILGPLKIDFSDKNMVEQGF
                     VFEQLTTGHQCGCGESFTPVKDLNTVK"
     gene            24197..24823
                     /locus_tag="EYO68_01995"
     CDS             24197..24823
                     /locus_tag="EYO68_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIC06605.1"
                     /translation="MFQFLEWLAGATCFGLFIYKWIIIAAVVMTWVSADPHNQIVQWI
                     GRVTRPLWVWCEQRMPVMMAHFSAYASILVVIFAQIVLPAEIRSLNLLFQGQTDFAGV
                     VLQSGGHLLQGMSIVVQSLLFFCIFVLIAWFVLTLVNPSLSNPLVRIIHVLADPLITP
                     VQSYLPRTRTDWSPLVSVLLFYLISSSIISPIGFYGSTLSNPVSICIY"
     gene            complement(24994..26289)
                     /gene="hisD"
                     /locus_tag="EYO68_02000"
     CDS             complement(24994..26289)
                     /gene="hisD"
                     /locus_tag="EYO68_02000"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIC06606.1"
                     /translation="MQVKIFEGFDLFSKSFDAKENKDVSVTVQEILETVREKGDSALR
                     EYTERFDNVIPESWLVPKPLLTEALENLDHELFHILEEAADNIMFFHERQKTESQLDF
                     SPDGTVLGWKVTPVDSVGIYIPGGRAAYPSTLLMNVIPAQVAGVPRIAMVSPPGPSGL
                     PHQLVMASAALMGLEELYSVGGAQSIGALAYGTESIQQVVKITGPGNAYVAEAKRQVF
                     GTVGIDSFAGPSEIMVVCDRDDIPVEYLVRDMLSQAEHDPDAGAVLVTTSAKQAKDVS
                     KRLKKLVPTLPRREIIEASFANRSAIIVAEDLEEIFEVINELAPEHLEVLTKQPFEDL
                     HRIRNAGAIFLGPNSPEPVGDYFAGPNHTLPTSGSAKFSSPLGVQDFVKTSSVISYSP
                     ERLVRQGEQIIRFAEEEQLFAHAEAIKVRLKKQQAAKKL"
     gene            complement(26396..>27367)
                     /locus_tag="EYO68_02005"
     CDS             complement(26396..>27367)
                     /locus_tag="EYO68_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIC06607.1"
                     /translation="RKRITEIDESFEDLLEKRSDMKRELEEDIQLHEQKNEEQTLLSQ
                     KLQERQGHLENSVSVSHQESIRLTEFRLQREQLEDQLREITEQTPEAILTEIDIDTSD
                     HKQMGLELRSLKASLNAMGAVNLAAPEEYAALKERIDFLKSQSEDLQKAVDDLKETIK
                     DINIESRRRFREMFDQVNENFMNVFSSLFEGGEAKLVLTESEDLLNAGVDIVAQPPGK
                     KLQNINLLSGGEKALTAISLIFAIFLVKPSPFCLLDEVDAPLDDVNVVRFNRLITGLS
                     YDSQFIIVTHNKKTMEIGDLLYGVTMEEPGISKTVSVEFKEAESLIA"
     assembly_gap    27368..27467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27468..30129)
                     /locus_tag="EYO68_02010"
     CDS             complement(<27468..30129)
                     /locus_tag="EYO68_02010"
                     /inference="COORDINATES: protein motif:HMM:PF13476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06608.1"
                     /translation="MRIKKIRVSGFKSFCHPVNLQVKQNGITIIVGPNGCGKSNVVDA
                     VRWVLGEQRVKHLRGSSMEDVIFSGSSFHKPSGMAEVSLTFSNPKGDTLQQFADYTEI
                     AVTRRLYRSGESIYMINKIPVRLKDVRELFMDTGIGGTGYSIVEQGRVEEIVSAKPID
                     RRILIDDAAGIVKFRFKRENAEKRLDETTQNLLRVNDVLGALSEQEDGLREHVERAEK
                     YLELQEQSGLLERQHLCLSWHQAGLNEQKVQELVKGQQQQQQDLQGEKAVVETDLERL
                     KLEQTQLGTQVGERREQLFKTEQNIQEAENQRELEKQNLQNVSEQREGQEKELEELKQ
                     RLTEREKERADINNRSMELAERLGEMQAKLVIIEEKRAEENETLQKSTDETSVLQKRL
                     LTVHTELTNQTNQKNFLDERLENLTERQQRLQDQQQSHKNLLQEVTLQVSDREEQMVT
                     MRLQKEELSGKLAELESSIEDQHLKLIEEEKKLEDLRYQQSTAESRIESLQQIQTHYE
                     GFSDSVKIFMQLMQDNPETKKQLGVSGLLADFITVPADTLDIVSPVMAEVLDWVVIER
                     AEKLPQIEIFCAEHELGQLGFVALDRPASVPKSAGTKGIPLPEILKFKKPLQEWGEKF
                     FSRFALLKDEKKFWNEADKKWPDSPEEWLSSSGICLSNSSASMGKVQSGSLGFLQRQQ
                     QIIDVGTYVDDLQKKIKKFESELLTLREKHESLKEEQESREEESRKLEFSLLSGNKEL
                     EHHQLEERRMEQTVSQLSQDTDSILEEMDLSRLKEKTAAETIISLEKERTELEEKTNQ
                     LQERIQLLQSRNDAIAEELLAHRVSLTETTEQQKNTEETESRLNREHEETSLRLEQLK
                     TALNEGDLRLEQSRKRITEIDESFEDLLEKR"
     gene            complement(30234..31007)
                     /locus_tag="EYO68_02015"
     CDS             complement(30234..31007)
                     /locus_tag="EYO68_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HIC06609.1"
                     /translation="MFESINVLSLFTEKQRAVKVKQKRMISMIMLDDIDAEVTKKKIK
                     HLHLKVCPPNGIVRVSAPLRMNQEKIRVFALSKLDWIRKQRLKIRNQVREKPLKYINQ
                     ETHYFRGHSYQLKVLENNKPPFAELVKNEILIHVPTGADMEKRRSVLNEWYHKQLMEL
                     LNPLIKKWELKLGVSVKRFSIRSMKSRWGSCTPKTHGIRFNLELVKKSPECLEYIVVH
                     ELVHLLEASHNNRFKALMDQFYPNWKLCRKELNGLQLKS"
     gene            complement(31241..31723)
                     /locus_tag="EYO68_02020"
     CDS             complement(31241..31723)
                     /locus_tag="EYO68_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIC06610.1"
                     /translation="MANLNKVMLIGRLGQDPEIRYTQSGSAVANTTIATNDYWTDKQG
                     EKQERTEWHSLVLWGRLADLAQSYLKKGSQVYVEGRLQTRDWEDQQGQKHYKTEVVVT
                     NMQFLDSKNVDGGIPQEGGSSNYSATSASTQDSASTQDPGNVQETPHDDGFIKDDIPF
                     "
     gene            complement(31763..32380)
                     /gene="ruvA"
                     /locus_tag="EYO68_02025"
     CDS             complement(31763..32380)
                     /gene="ruvA"
                     /locus_tag="EYO68_02025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIC06611.1"
                     /translation="MIAYLEGQILRSEANLIVLKTSDGVGYAVHVSQHLAVKASPEEF
                     ITLHIYTNVREDDISLYGFQKLEEKELFEMLIKTSGVGPKLGLAVLSVLSPSQLVDAV
                     HQSSTEIFSQVPGIGKKTAAKLCLDLKDQLKRHPISGFEPGPAGSSKAGLTLSGTEID
                     GVFSALKNLGYSEKEILPVLRETGSSDLPFEGRLKKALSLLTPLR"
     gene            complement(32428..32925)
                     /gene="ruvC"
                     /locus_tag="EYO68_02030"
     CDS             complement(32428..32925)
                     /gene="ruvC"
                     /locus_tag="EYO68_02030"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIC06612.1"
                     /translation="MVETLRILGIDPGSRKTGYGLIENTGNRTRHLASGCIRLNAKHP
                     LSDRLSMLSRELEQLIEEFRPDCGAVEKVFFAKNAQSALTLGHARGVILLKFSERHLP
                     IHEYQTLKVKQTVVGVGRADKDQVQHMVKILLNLQNSLQEDEADALAVAITHAHLGLS
                     LKQSL"
     gene            complement(32918..33670)
                     /locus_tag="EYO68_02035"
     CDS             complement(32918..33670)
                     /locus_tag="EYO68_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIC06613.1"
                     /translation="MSGHSKWSTIKHRKAAQDAKRGKAFTKIIKELTIAARIGGSDLD
                     ANPRLRTAVANAKSQSMPKDNIDRAIKKGAGELDGVIFEEIIYEGYGPHNVAIIVEVM
                     TDNRNRSIASVRHAFSICNGNLGSANSVQYMFDRQGSILVDNTVIDEDRLTELILEAG
                     AEDLNTENPDAYQIITAPADFDTVRSYLEENGVAMLSAEVIWNPQNRIEIDNYKKAEQ
                     VIKLIDLLEEDDDVNSVHSNFDISESVMAELG"
BASE COUNT         9362 a         6975 c         7061 g        10303 t
ORIGIN      
        1 tctttaaatt tttccagcaa aacgtctttc cttgagtcca ggtaatcttg cggactaatt
       61 ggcaagtcca gactttcaac tataatttct gcggcctgaa tagatctacg gccaataatc
      121 ttttccttaa cggaccaatc gaaaaccttt ccgtattcac ctacaatttg ctgggtgact
      181 tcggtgtata tgccttctgt atccaagagg aggccgtcca tatcatatat caaacaggaa
      241 atattcggag acattgtttt atgtttaaaa tgtaagtgat gaaattgaga aaggcagatt
      301 tacataagag agtcttaact ctgcaggaac gaaatttttc gagtcatccc gaacgaatgt
      361 gagggatctt gcacgatatc agtattttat cagatctctt ccttccttcg atcgaaaaga
      421 caagctgtag tcaataatat tagtatctta cgagaactcg tgactcacga ggtcttaagt
      481 gctgttatta aaaaagttcc ggtacatatt ttagcaaatc gacagtgtat ttttccccgt
      541 tactcaattt gtagctgaca gaattttcat cttcccaaac cagtttcagg cgataattta
      601 ggtcctgaat taggagattt ccaatacttt tagaatcgcc aagttttcga ttaataacat
      661 gaagcctgaa ggtgtaaagc ctggttttta tttcctgtcc gcttgcattg agacgtatga
      721 cttcttttga gtcggctata tgataaaata taacatgagt tgcagcagaa gttactccgg
      781 atccggtccg gaggtaaggt aatatgggtt gaatattatt ctgaaatatt ctaaaaattc
      841 ttttaagtcc ggtaatgttg tttgaatact ggtaaaattc cgggtcaatc tgccaaaaac
      901 gtgcgtctct gtctgcctca gcttttacaa tgttaagccc caaaactcca ttttgatctc
      961 gaaagaggta tacgtggctt tcagtacttt taatgttaac aattttaaga atcacacgtt
     1021 gggaatgtga gtctaaaagc ttcttctcga gataaatccc gttgcccatc ctgttctcag
     1081 gaaagatttg atatggtgaa agatctatta ctcccctgtg agtagaatct cttaaataac
     1141 tttcaacggc ccaaattcca tttggggaaa atatccacaa aagcccggaa caaacaataa
     1201 aaaaatattt tttacaatta aacatagatc gttatggctg tctcaattct atcttttcct
     1261 gatgtcttat aagttgggtt tattgaaaag ttttaatttt atcatcccgt acttgatccc
     1321 ggatactggt ctctgctcat gttgagaaca ggattttgaa gctgtatccc aattggttta
     1381 tactttatgc tccgcaccaa gcagtgacaa taaaacggaa ttgaaagcgt aaatcaaggt
     1441 aagcttttcc ggctttaatt tttcattcta caaaataaca tctttcgtag cacattgatt
     1501 atagcaaact tttttactga agccagtgaa atatttcctg tggaacttga gtcctgtcca
     1561 tgttgatatt agaataaaca tatcaagcag tttgaactgt tttaataatt gcacacgcaa
     1621 atcaataatt cttcaaacat tcttaaatac cattactacg aagattgtct ccaacccgtt
     1681 cgaaaagcag gaacctgttt tttcaattac tgagagcaac tgtattcttg cactcactgg
     1741 agttacacat ttttagggga acatcttact taaaatacaa gaaattatac aaaccaccac
     1801 attaaaaaat ggattcaaat aaaactgcag cgctttcctc ccggcctttt gttatcggaa
     1861 ccagcggaca tattgaccat ggaaaaacta ctttggtcaa ggctctcact ggagtggata
     1921 cggaccgctg ggcagaagaa aaaaaacgtg ggataaccat tgatcttggt tttgctcatt
     1981 atgaggatga acaaggaaat caaatggcat tcgtggatgt cccgggtcac gaaaaattta
     2041 ttcacaacat gctggcaggc gtggcaggat tggatgcagt tttactcgtt gtagctgcag
     2101 atgaaggggt gatgccacaa acacgggaac atctgaatat ctgtaatctg ttgaaaattc
     2161 aaacagggtt ggtggtactc acaagaattg atttggtgga agaagaagaa atggtcgaac
     2221 tctgtcgtga ggaagtttca gaattgctga aaggaacatt tcttgagggg gcgccgattt
     2281 tagctgtctc tccagtgacg ggtgaaggat tggatgaact ccgtaagaca ctgtcaaaac
     2341 taccgaaaca aatacagcat ccggcaacag aaaaaccatt caggcttcat gttgatcgtt
     2401 ctttcacgct aaagggcttt ggcacagtcg tcacgggaac tgtgctcagc ggaaccgtta
     2461 gacaagatga ggaaatactg caatatccac aacggcggaa cgtacgtatt cgaggattgc
     2521 agaaccacgg ccaggaagtc aaagagatta atgcaggaca gagggccgcc ctgaatttag
     2581 caggaatcgc caaagaagaa attcagcgtg gtgaccagtt ggcgcatccg gaatcattac
     2641 tgaacagtta tttgcttaat gtgtcattaa ctctgttgga agacgtttta gccccactta
     2701 aaaacaggac ccgtgttcgc gttcatcttg ggagcaagga agtttttgga cgaattactt
     2761 tgttggaagg tgaaactttg tctcctggaa aaacagcgct ggttcaatta cgtttggaaa
     2821 gtttggtaag cggtagttat ggagaccgat ttatcgtccg gagttattca ccaatgatta
     2881 ccttaggtgg aggaatggtg attgaccctt ctcccggtaa atccaggaga gtacagcacg
     2941 aacttcctga acggctggaa cgtcttcatg ttggtgatga aaatattcga tctgaagaag
     3001 tgatctattt gcagtctgtt cggggagtaa ctgagtctga attttcggtt cgtaccggcc
     3061 tttccagcag gcagtccgga aaagctttgc agcttttgca aagtcagcag aaagtacttt
     3121 gtgttgaacc tctcacagga cgttatctgc atgtggagca tattgtgcga atcggcaaat
     3181 ttttgagcaa agcgttggag cagcatcaca aaaaattccc tgatcgcgat gggatgaccc
     3241 gttctgaact tggtggtaaa ctttccttga tttttacaga aaaagaggtt gaaaaactgc
     3301 taaaatatct ggtgaaggtt ggtctgttta cacaaaatga tcagtatatt tccttagcag
     3361 aacatcagcc gcaggcatca caaagcaggg aagacagtct tgcacgctgc cttgaattga
     3421 ttaagtccgg tggttttcaa cctttacgcc gtacacattt actccaggaa ctgggtttaa
     3481 acgaaaagga tggaatgaca ttactcaaat ctgcagcaca ttcaaaacga cttgtacggg
     3541 ttgcagagga tttacactat attccagagc aaattgcatc aattatagaa agtctgcggt
     3601 tttattttag tgaaaatcca aacattactg taatccaatt caaagaatta ctaaacattt
     3661 ctcgcaaaca tgcaattgat ttgttagagt attttgacag tcaacagctc acaattcggg
     3721 aagataacca ccgaattccg gccagaatta cagctttaaa caattaaaga aaacggcata
     3781 caaagtgttt ttacttcaag ttataatttt cccaatttta cttgtcactt tactgagcgt
     3841 gtggaacttt caaacagtaa ctgtaacagc tcatgcccaa agtttgctga gtgacagcga
     3901 gtggttggag gaaaataagg atgttttcgg caaacaggag cttactttgc aaaatattcc
     3961 gttgagacct tctgacttgt cctccactcc tacagttctg ccggaatcgt tcctaattcc
     4021 tcttgaaacc ggcggacaat atttttcaac agttcctcaa aaacctgccc tgcctttgat
     4081 agaacagaaa gatcaaagcg aaaatccttc aggctctttt ttaagcagac tttttccctg
     4141 gtttagcagt acagagcaat ctacatccac taacctggat actgcttcag aaatgcataa
     4201 tgcagaaact agggtggagt gggctgattc agaatttgcg ggaataaaaa tgctgataac
     4261 agaaattgaa aggctgctca tatcagatcc ggtagcggcc agggaaatgt acgaggaaat
     4321 agaagatcag ttggagattg atgagcgtac acggctgaaa gtacaattgc tttattattt
     4381 gaaaaagtgg tcttctgcag agcagttagc agaagtattt ttgagcgaac gtccagaaag
     4441 taagatggcc tctttgatgt tttatttttt gaacaaatca ctgatggctc agcataagcc
     4501 tctcagtcaa aatttgattt tcaggaaacg tgcagtggaa actctggaag caaaattccg
     4561 cagtgatttt ttgttcattc tcagtaacga agcatttctt gaaggcgatc ttttaactgc
     4621 aatacaatat cgccttgaag gaatgaataa agcaaaaact gctaatgttg ccagtatgga
     4681 aaaattgaat ggtttgttaa atcaagttca gtctcctgaa atactgcgaa ttttatcaga
     4741 gaataataag aatgttaaat ggctgcagaa aaaaatcttc ataatggaat tggaacttct
     4801 ggctaaactg aaacgttacc gtgatgcatt ggggatttta gagcaaagaa tgtctgctgc
     4861 aagggtaata ggagatcagg aagagcttga agttttgaag caaatacaga tccgctatac
     4921 aaatgctttg aacgtcaatc ctcgccgaat tggggtaata cttcccttga gcagttccag
     4981 cgtaaaagtt tcccgattgg cacagcaaac tatgaatggt ttgtggctgg cacttcatgc
     5041 aaacgaaatt cctgcagtgc ctgaaaacat gggtgacgat gaggcttcgc aagaaaatga
     5101 aataaccggc aaatcagaag ataaagacaa tgctcaggcg gaatctgatt cgaggaaatt
     5161 tgaagattct tgggaacttg tggtacgtga ttcacagttg aatccggaaa taaccaaaag
     5221 cttggtacgc gaattggtgg agacggaaag ggtgattgcc atcattggac ctcttgcccg
     5281 gaaaacatca gaggctgctg cagaagaagc tgagcgtttg cgggttcctt taatttctct
     5341 ttctttgaca gaaagcattc cggaacttgg tgaatttata tttcgtaata atcaaagctg
     5401 gaaacaggaa atccaagaat tgttggaata tgcaacgagt gagctccagg cttgccgttt
     5461 cctgatactt tatgcaaaaa cacgggaagg tcgacaaaaa atgagactat tttgggatgc
     5521 cgcagttctg aagggatgca aagtagttgc agttgaaggt tttaaggatg aaggtcaaaa
     5581 aagtctagtg aacgaattcg atactttcac aggcaaaata agacgtttgg gtacagaaga
     5641 caaaataatt ttgaaggaac ttaaagagaa ggaagtgccg attcacaact ttgatgccgt
     5701 ttttgtagcc gttggttcgg gtggtgtaaa aaatttaagt ctcatctttc catacagtga
     5761 agtgtacaaa atgagaaaaa cgacttttct gggagatagt ggttggaatg attcagccct
     5821 gccgtatgct ccgggacttc gaggagtgaa aaaccccgta tttgtggaca gttttttcct
     5881 gcaaagtaaa acaccggcca tgcagcaact gttaaggctg catgaacgga ttttatatcg
     5941 gcatcaaaat tatattggtc ctacagctta tactgctttt gcctatgata cgctgataat
     6001 cttgatgcgg ttgcttgaag acgagcgaaa tcagagccac cgcgatctgc gggatgcctt
     6061 gttaaatatg cagatgtttc ccggtgttac cggcaattta cgcttcgatg aaaggggtaa
     6121 agttgaacgt gaaatgcagc tgttgacttt gcggaggggg aaaattcagc ctttgaatta
     6181 aaagtattat gaggctgaat atgtccaggc agcagatttc actgcctgat taagttctga
     6241 ctattttaca aaaagtgatt caaggtcctt aaatgggact tgttcaggct tcacattttg
     6301 ctgcggttca tttgtatttc ccgtgttacc agcgtcgcct attgcagatt ctggataaga
     6361 atcattggga ccaccaatca tcaagtcatc ctcttttttc tcaaggggct tttctggtcg
     6421 gatttctgcg taaaagttgc gctttgactg tgctttcata agaatttttt gttcttccag
     6481 gggctcttca accaaatctg caacttgccg caaacgataa ctatattgac gactaacctc
     6541 taaaagacgt ccgccgggaa tgcactccgg actgtcctgg aatgtcccaa aatcatctac
     6601 tatgactgcg tgttttagag cttcaggacc caaataaact aaaatacatg agttttcagg
     6661 atctgatact tcctcttcct catgagtttc aaattttaac catttcatat ttgcctccta
     6721 ataacttaaa tattgttaaa tcattgatta aacccctgcg aaaaacatgc cattccctgt
     6781 ggattattag aacattttac tctggaatat tgaaaaactt agcgggatgt ggattcgcgg
     6841 aataatgaga aaatatcaat aataattaca gttatataga tagtaataac ggtttataca
     6901 atttttcttt atcaagttca cctaacatga attggatatt gctttatggt attcagagaa
     6961 taaatttgat ttatttatat ttgaatatta agatatttac taatgaaaag gagataccat
     7021 gaaaacttca gcaagtaaaa acgaaatatt aaaacgtgag ctcgaacaac tatttggcga
     7081 gcaggatgca aatattgaaa ctgcagcaca gtgtttaaag gttatgtctc atcctgtccg
     7141 cctgcgtatt ttgtgtgcct tgcgcgaggg agaacaaaca gtccagaaac tggaatacta
     7201 cactggaatt aaacagtcga ctctgtcaca gcacttagct ttgttgaaaa gccgaggcgt
     7261 tttagtttca cgacgtgaaa caacctactc tttttacagt ttttccaacg atcaaattac
     7321 acaattattc gatttgataa aggaagtatt ttgtacctga attaaaatga caaattcaat
     7381 ggctaaacca aaccagccca agttggtctt ccgacaattt tttgacaagg atacgggaac
     7441 attcacgtat tttttgtttg attctgaaac tatggaaggc ctgataattg atccggttaa
     7501 agagcaattt gatctaagtc tacagttcat agaagaactg ggagttgagc taaaatatgt
     7561 aattgatacg catgtccatg cggatcatgt tacttcttca tgtatgctgc gtgaagctac
     7621 tggagcaaaa agtgtttttg gtgagcctgc cggaattgaa tgtgcagata ttcttcttga
     7681 agatggagat gagttagagt ttggacactt tagcctcaaa gcaattgcga ctcccggaca
     7741 tacagatgcc tgtaccagtt tttatacgga agggatgctc tttacaggag actctttgtt
     7801 gattcgcggt tgcggacgca ccgatttcca gttgggtgat ccggaaaaac tttatgaaag
     7861 tattactcaa aaactgtact cctatccgga cgacaccttg gtttatcctg gtcatgatta
     7921 tctgggacgt acagcctcat gtatcagaga agaaaaagct tttaatccca gaattggcag
     7981 cggacaaacg ttggaaaaat ttgtccaaat tatgaaaaat ctggatttgc caaagcctaa
     8041 acgcattgat tcgactgttt ctcagaatct tcaatgcggt ttttcactgg aacttggtca
     8101 tgtaaatgaa gataatttta ctatgcatga tttgtatcag ttgctggata atctgaaatc
     8161 tacagagagg gtaattgatg tacggacgcc caacgagtat tctcaaggtc atgtccccgg
     8221 tagtttaaat attcccatgg gtaacgaaca gtcgtttctt gaagagctca gcagttatca
     8281 aagaatattt gtacattgcc actctggacg cagggcacaa actgtttaca caatgcttgg
     8341 aatgcagtgt ttggaaaatg ttgtctgtat caacagttcc ggaatggcag actggacgat
     8401 agcaggcttt cctgtggaac ggtaagggag ccaccctcaa tgaagaagag tatcctggtc
     8461 aaactcagtt taattcccgc tttattactt attccaacta tactttgggc cgcttctacg
     8521 gaaatatctg gaatgccctg gtggggctgg ccgacggcct tatttttcac aacctttttc
     8581 atgggtgttt tcggtgtaat ggccggaatt ggcggcggtg ttttgtttgt ccctctggtt
     8641 agtggttttt tcccgtttca catcgacttt gtccgaggtg caggcttgtt tattgccctc
     8701 ggcagttcac ttgctgctgc tccacattta ctggaaaaaa atctggctag tttgaagctc
     8761 gcgctaccgg ctgcgttgat tgcctctgcc tttgcaattg taggggcatt tctgggactt
     8821 tggctttctg cactgaatcc ggcatatatt cacgggagcc tgggtctaac gattatcact
     8881 atcgttttca tcatgctgtt tgcaaaaaac acggagaacc ctgaagtgag caggggaaac
     8941 aaattgaccg agcttttgca aatcaatggt atttacaccg aagaaactac gggcaaagaa
     9001 gttagttgga ctgttcacca cacaaagaga ggtatgttcg gttttgtggt cgtcggtttg
     9061 ctggcaggtt tttttggatt gggtgcaggc tgggcgaatg ttcccatttt gaacttgttg
     9121 atgggagcac cggtaaaagt cgcagttgct acaagcagtt ttgcgctttc aattacggac
     9181 agcacagcgg cctggattta catgaataaa ggagcagttt tgccaataat agttgttccc
     9241 tcaatggtcg gtatcatgct gggggcaaga attggttcaa agttgcttcc aaaagttaaa
     9301 ccaactgtaa ttcgttacat cgtcatcaca gttcttcttt tggcaggagt acgttccctg
     9361 ttgaaagctt ttggtatttg agcacaaact tggagaacta atggaaaaat ctatagtttt
     9421 tgaaaatgct ccagagcagc atatatacgc aaaaattata tatcacataa gtaatctggg
     9481 tttacttgtt ctgattatgg gattcatttt gtatattttc ggagtgctga ctccgctcgt
     9541 tccgctggaa gaactgccta agtactggtc attatcttta acgcagtttc tggaaaaaac
     9601 cggagcgcct accggctggc gatggacagc aatgttaggt tatggtgatg taataccgtt
     9661 tttaggggtt accattttgg cctccgttac ttttgtttgt tttctagctt tgttgtttag
     9721 ttttctgcaa agaggagcca aagtgctggc atttattgcg gcaatggagt tgttcttcat
     9781 tcttatctct gcctctaatc ttatccagat ttcccactga aatctctcaa attgaagcct
     9841 tatcttcttc cactggcagt cgcccagaaa atccctgccg aaatcaataa gtccaaaaat
     9901 aaagttgata ccttactaag aaactctgca gcttagacaa gggcttccag cagccggttt
     9961 tggtatgcag tgatcaaagc ttcgccataa tggggtagct tctccaggcg gcgttttacc
    10021 ggtgttttgt ccgtttgcgg gcgcattacc tgatgtaaag tgtaacgcag aatatcctgg
    10081 ggattttgaa tacagtaaag aatgaatgtc aaagcatcgt gttccggttt tttttcgtaa
    10141 atgagccaca acagcctaac tatgtgaaaa tgattgattc cgcgtgaaca ttgccggtcc
    10201 acaaattcca ggattgattt aaggtaatta tcggccctga aaccttgcgg tagccctgct
    10261 gattcagtga actcctgata ataaagcaaa gtcagcaccg aagagcagaa atagctccag
    10321 gcttttctaa aatcatccag aggtatcaac tgtctttgag gaaccgtgtc cggttctgcc
    10381 tgcagtactg cctcctcgtt ggtgtccagt aacactccaa gggaactctg tattgacttg
    10441 attgccggca atcgaaattt cataactgac ataagccttt tcaggcgcag accatcagca
    10501 ctttccaaaa aacctgctgc ccttgattcc tccggcgact gtgtccacgc acttaaactg
    10561 tgaagtgcag aatacagttt agaataaatt gactcggcat agtgcttttc catctttttt
    10621 atttcttttg accattcttt caaaggggtt ccggaaggaa gcatttttat caggcgcaat
    10681 ggaaattttt gccgtatatt gtccaattcc gaattttgcg gcatctccgg aacataaaag
    10741 agaatcactt ttttctcttt tctcaactgt cgcattctgg atcgaatttg tgtaccgact
    10801 ccgtctaatt ctacagcttc cggaggttgt ttcaattgtt cgtacagttc ttcaataaat
    10861 ccggtaagtg cagaaatttc ttccaatttg tgaagcacag agcggatgta atgtgagaat
    10921 cgttcttgtt caaaattttc ataaacccgc atctcatggg gcagaatgaa atgtaaatgt
    10981 gaattcaact gcttgactcg ttcactcact ttctgattaa attgctcggg atttgtccgg
    11041 aacaattcct taaactcggt aaaaatggag gaaagtgtat gttgttcttc ggttgagagt
    11101 gactgggctt tccacttttc gcgccactca tctattaatt gcttccggat ttgatctccc
    11161 tctttttcca tggaaagacg gtatgacttt gcaacacttt cacaatgtcc ttccaaacta
    11221 tctgaatgaa aattaaattc tttgaccagg tccagtattt caggaattgc ggcagtaatt
    11281 tgtttttgtc ctttcacact ttgctggtaa aactcaagag gcaggatttg ctcattgaag
    11341 aatatatttc cgaataattt tgaaaataca ttgcgagtaa tgttgacgat aacttctgca
    11401 gtttccagtt tttttggcag aatgatcgtc accttgaatg atcttgaatc aatgaaggaa
    11461 tctattcgtg aggcaggaag tgtaacattt tttggtagag gtgagccgat ttcagtctcg
    11521 tatgttgtct gatcaataaa acgcagcaga tggctggcgt aatttatttt caaacctaat
    11581 ctgcctggaa aatcaagcat gtcgggaagt ttttcgctca aaaatgtttt tgtctcaagc
    11641 ggaatcgatt cagaaaattc taaccgaaaa aattttgagg tgggagtttg ttctttttcc
    11701 tgatcatgat tttggagttg ggagtctcca attgtacgga gcttaggatc aaagcgctct
    11761 tcaaaagatt tgtcgttgct gtcaaaggac ttcatgaaat tgtaccattg aatggcctgg
    11821 ctcacgaccg gggatcaatg cttcctgaca gaagctatat cttcgttcca ttctctcagt
    11881 ttatgcggac cttcgagtgt agttttcagt tcgattaaat ccaaaaactt tttatcttcc
    11941 aaatcaacat tgggatacct ccttcttaaa tagccccaga ggttaaggta gttctcctct
    12001 atttgccaat cttcattctg cgacttctgc tctgttttgg gctgttcagt aaatgggtaa
    12061 aaaaccttgc tcatactact ttctcgaaaa ctctgtttat tatctttcca aactcgctgt
    12121 tccaaaaaac taatccggaa atttagaatt tatcaccaat gctgccgaaa gttttaacgg
    12181 ccttatactc tatgtagcaa tattgttgcc tgaaaatata atcaggatag gaaaaagttg
    12241 aataatctat taaatactgg gctaatatat tgttaataca gactaattta ttattatttt
    12301 aaaataaaaa aattataaaa taattttcat aatttacaat tgcgttgatc aaagagcaaa
    12361 acaatttgtg gatttgggag gagaattaag atgtaaatct atttcattaa attcccaatt
    12421 acttgtaaaa aatacaatat gaaggtatat attggtttca actaaaacca ccaaatttgg
    12481 gtttctatta agtggataac gattcatttg aaaaaataaa tcagtacagc aggcgtgagg
    12541 tgctgaaatt tctttcatac gcaggagtga tgggtacaat cgtgtttctt ctgaacagtt
    12601 gtactctgcc tgccggcatg agacaaatca ctggtgaagt tttcattaac ggacgtagaa
    12661 tccggcccga ccagttgaaa ggaactgatg gtgtgcttgt agaaaatgat gcttcgatca
    12721 gtacaggatc agccggatct gctgtctttg ttattggtag agatgcattt attatccggg
    12781 gaaacagcag actggaactt aaccctacac ttgacaggaa acaaaactaa atttccggat
    12841 tcggtctcag atcatgagca atactttcag tttttgccgg gaaaaagcgt acattgagaa
    12901 ccgttacagc cgttattgga atcaagggaa ctggagtcta tcttgaggct gattctgaaa
    12961 aaacctacgt gtgtacctgt tacgggactt cccatttgca ggttaaggat gcccccgaaa
    13021 tcagtgaaac cgtcaccacc agtcatcatg accaaccacg tttcatctac tctggagaaa
    13081 aacggattga acaagcaccg gtgatcaacc attcagatca agaactgata ctgcttgaaa
    13141 aattggtggg ccgcattcct cctttcgtaa aaccgaatag gaacgatgga ggcaaatctt
    13201 caaagtcaga taacccttat tcatctaata aatacggata ttgatccatt aattaaggtt
    13261 tcctgaatac caaaccggaa tcgcttttat tgtgttggag agaggcttat acattttcac
    13321 cctgttccat tttcccgttt aatatggaat tgaattccaa tatacaggga ttattaaaac
    13381 cacagcaagt tcagaattac tgtcttaagg tgaaggacaa aaggggaatt atgttttaga
    13441 ttgaagcgga ttcagatgag tgatactttt atggaagcta aaatattttg cacatgaaat
    13501 caccatccca aaagagccct tatgagagat cgaataatat ctattaaagc catggaaatc
    13561 ctggattcac gcggaaatcc aacaattcgc tccacagtaa ttcttgaaaa cggagtaacc
    13621 ggaactgcgt ctgttccgtc cggagcatca actggtgaaa atgaagccct tgaactcagg
    13681 gatggtgaca aagcccgtta tgccggaaag ggagtactca aagctgtaga aaatgtgaat
    13741 caggaaattg caccggaact gctgggaatg actccggatc ggcaggcaga aattgacagg
    13801 atgatgctga aactggacgg cactgcgacc aaaagtaatt tgggcgcaaa cgcaattctc
    13861 agtgtttcca tggccgttgc ggtaactgcg gccaattcac acaaccttcc actttatgct
    13921 tatctcggag gtgtgggcgg attccgttta ccgatgccga tgatgaacat tatcaatggc
    13981 ggagaacatg ctgacaatag tgtagatttg caggaattca tggttatgcc gattggcgca
    14041 ccaacattcc gggaagcctt gcgttatggc gcggaatcat ttcatgcgct gaaaaaaatc
    14101 ctgcttaaaa aaggctatgc gacaagtgta ggtgacgaag gcggttttgc gccaaatctt
    14161 aaaagcaatg aagaggcatg cgaagtaatt cttgaagcaa ttaaggctgc cggatatgaa
    14221 cccggaaaag acatttcact ggccatggat gccgcagcgt catcttttta taaaaaaggt
    14281 tcgtatgact tttttaagtc cggacagggc acaaaaaatt caacggagat gctgggattt
    14341 tacagtagta tggttgaaaa atatccggtt gtatcaattg aagatccgat ggatgagcat
    14401 gattgggacg gtttttcagc cattaccaaa aaacttggta aaaaagttca aattgttgga
    14461 gatgatttgt ttgtgacaaa cacagatttt gtcaaaaaag ggatcagtaa aaatgcggcc
    14521 aatgcggttc tgattaaact caatcaaatc gggacagttt ctgaaactgt tgccaccatt
    14581 cagctttgcc aaaaagcagg ctggaattat attatttcgc accgcagcgg tgaaactgaa
    14641 gatacgttta tggcagactt tgcagttgca atggccggag ggcaaattaa aactggctca
    14701 gcttcacgca gcgagcgcat tgcaaaatat aaccgcttac ttgaaatcga atcagaactc
    14761 ggtaagtccg caattttcgg tagttgaccg caagtctgcc ggggtcgtga ttctgaaccc
    14821 aatctgtcaa cattgaacca cttgacattc attcctttga aaaacaatcc tgaggagtcc
    14881 ggacgcattc tgaaaaacgc gcggacggtt acaatcttta cccttttttc acgaatcctg
    14941 ggtgcagcgc gggatttagt gattgcgcat gtctttggtg ccggttgggt gacggatgct
    15001 tttgtgcagg cttttacaat tccgaatgtc ctgcgccgcc tgactgcaga gggttcgatg
    15061 actcttgctt ttcttcctct ctacacagaa atccgtgaac gcaaggatcc tgaagctgca
    15121 aagaaatttg cggcaaaaac tttagggctc gtcctggcag caacaactat tctgaccggc
    15181 ttgggaattc ttttcagtcc tcagttggtt tatctgtttg cggcaggttt tgcttcaagt
    15241 ccggaaaaat acgacttgac ggtgctgctg accagagtca tgtttccata tttgattttt
    15301 gtttcgctcg ttgcctgggc aatgggggta ctcaatgccg aaggccgttt tgcagctccg
    15361 gctgcagcac cgattttatt aaatatcgga attatcgggg cagcattcgg tatttctcca
    15421 ttgttggaag aaccgatgat cggcatcggt attggtgttc ttctgggtgg aatctcgcaa
    15481 atattaattc aaataccctc actgagaaaa gttgggcaat ccttcaaacc gcaaaatttt
    15541 ctcaatgatg agaatattca gcgcctgctg aaactgcttg gtccgtcttt gctgggagtg
    15601 gcggtctatc aaatcaatat cattgtgctg cgcaacctcg ccagtttcat gcccacaggg
    15661 caagtcacgc attactacaa tgccagccga ctctcggaac tgactttggg tgtatttgct
    15721 tttgcaatca cttcagccgg ctttccggaa ttaagtcagc acactgctgc caaaaactgg
    15781 gagaaaatcc gcaacaccct gcgctttacc atgtccacaa ctttactggt catctttccg
    15841 gccagcgtgg gtttggcggt tgttgcagaa ccgattgtgt caatgctata tctgcatgga
    15901 gcgtatgcat ggagcgacgt tcaaaatacc tcgctcacgc tgcaggcttt tgcattgagt
    15961 attcctgcgg tagcaatgat tcggttgcag acttcagtgt ttttttcact taaagatacc
    16021 cgcactccgg tcaaagtttc attgttcagt attttgctga cagggcttct gggttggtgg
    16081 tggagtcaaa gcctggaaat atttggcctg gccctggggc ttgctgcagg aacctggttt
    16141 cagtggattc tgttgtcgct ctttttgctc aagcaatccg aattacgcaa aaattggtgg
    16201 cccctgcggt cgtctttgct ttatatgctg gcttcagcag gaatgggagg ttttgcctgg
    16261 tattacagag gatatggatc atgggaaaac ggaccttttc ttgtttacaa ctgggtgatt
    16321 tttggaggaa tactgcttgg ttcagctttg ctttacggat tattgctttt agttttcagg
    16381 gaagagcaag tgctgagatt gaaaaaccat atgctacaaa ttaacaccta ataattccag
    16441 cgttgatgcc gccatcactt tggctgccgt aaccatatca tcaatcacta tgtattcgtc
    16501 cggctggtga gcaaggtcaa ggatgcctgg gccgtaggca atacagtcat gcagatgtcc
    16561 gatgcgggca atgtgttttt gatcataggt accgggagaa atgacatgtt caggagtttt
    16621 gcccaggatg ctctgaattg cagaactgac ggcttggaca accggggcat ccgcatctgt
    16681 catggttggt aaaaactcca taatatcctt gatttggtaa cgaaaattag gacgtgaatc
    16741 ggctagttcc ttcaacaagt ctgtgacttc tgctttgact tccgtgatat cttcttcaat
    16801 taaaaaacgt ctgtccataa tgagtctgca ggagtccgga acacaagggc tcggcaagcc
    16861 ttcaaaagat tcggcttcac ctccgtgaac tgcctgcaga ttcaaagtgg atcgccttgc
    16921 tccttctgga acaaccggca ttcgtgtttg ttttttgtcc agtgcaggaa aaagccgtga
    16981 ctcaattagc tccaaaaatg cactcatgtg tcgaatggcg gaatcgccga gaaaaggcat
    17041 tgagccgtgt gcgattcgac cgaatgtttc aacttccgcc caccatgcac cccggtgtcc
    17101 gagacagaca cgatcaacat tcaaaggttc cggaataatt acatgatcga cccgcggttt
    17161 tgagaaaaac cctttttctg ccaaaaaggc aactccaccg tatcctccgg tttcttcatc
    17221 aacagttccg gaaatttcga ttgcgcccgg aaaatcaatg ctttcttcca ggatagcctc
    17281 catggcaatt actgcggatg ctagtccgcc cttcatgtcg caggtgccgc gcccataaat
    17341 ttttccgtca cgcaccagtc cctcaaaggg gtcaacagtc cagccgctgc cagtaacaac
    17401 aacatcaagg tgtccgttga aatgaacaca tggtccggaa tgtttgcctt caatacgtgc
    17461 aatcacatta tgacgggggt aacgctctgt gtctccgggg gtattattgg cgcgttcata
    17521 aagcagtgaa aaaccacttt tcgccaaacg gtttcccagc agttccgcgc atagtaaata
    17581 atcctctccc ggaggattga tagttggaat acgtactaat tcctgtgtaa atgaaaccag
    17641 ttcttcacgt ttggagtcga ttctggcaaa cagtgattca atcatctatg accctttgat
    17701 ggagatttgt aatcacgaca tgataacaat atcaatatca ataagacaat cttcaattga
    17761 aaagtaagta aatgatttca gaaacaggag agaaaaccac ggggatgaac attggaaacg
    17821 gcaaactgta gatctcttgt taggaggctt aaaaacaaat gacccagctg cctgccgttt
    17881 cctttgaact tctggtcaga ttcttaacag ggatgcggta aaatttggga aagcatgagg
    17941 ggtccctttg cttttcggtc aatctcccgc aaccataaac taatatctgt gtccagtgtg
    18001 ttatactatg ccacgggata attttgattc atattagtaa atttacgtag attgcaaaat
    18061 aaattaaatt agatgcaata tcagtatttt aaataaatac tactactaaa attcatgtga
    18121 ttttcggtga gtatgtagag tgccggagaa aaaattctaa aggaatttaa taattaggtg
    18181 acttcgtgtt tctgcctgaa acgttcttcc cgccattctt ccgttttgag tacgtcagct
    18241 aaagcttccg cggcatccca cacatcagtg tagcgcaaat aaaggggagc aaaaccaaaa
    18301 cgcatacttc ccggctctct gaaatcggcg attatttttc tggcaataag agcctgaatg
    18361 acagcaaaac cattttcaaa atgaagggtg acttgagagc ctcgtttatc atgatcaatt
    18421 gggctctgca gagttacacc ataattacga catccacttt caataagttc aataaatagt
    18481 tgagtcagac gctgactttt ttttcgcact tgctgaagat cgacttcctg cgcaatttca
    18541 agaccggcct gcagtcctct gagtgataaa acaggttggc ttccagtccg gaaacgtcga
    18601 atgccttctg cagcatggaa attttcttca aactcaaaag gacgtgcgtg tccccaccat
    18661 ccggaaagag gattcacagc ttctttctgg tgccgctttg ccacatagat aaaggcgggc
    18721 gatcccggac caccattcag atatttgtaa gtgcagccaa ccgcaaaatc cacaccccat
    18781 tggtcaagtt ctaccggcaa aactcctgca ctgtgacagg tgtccagcac aaataaagct
    18841 cctgcgtcat gagttttttg gacaagttct tcaatgtgca ggatttcccc tgtacggtaa
    18901 ttaacatgcg aaaacacgac taccgcgacc tgattgtcaa ttaattcttc cagtgagttt
    18961 ccatctttgc ccaacaaacg cctttgaatg ttttgatgag ttgacgtgac tccttccaat
    19021 atgtaaagat cagtcggaaa actgtcagct tcggagatca tcactgtgcg ttccggacgt
    19081 agggtcaatg cggcatggac ggttttaaac aggtttagtg aagtggagtc agaaacaata
    19141 acttgtccgg aagcagcgcc gacaacaggt gctattaatt cccctagagt ttccggcaac
    19201 tcccaccagc cggccttgtt ccaacttgtt atgaggtctt cggcccactc tttccggata
    19261 gcctgctcca gttgttcgag tgcctttttt ggcatcgggc ccaaagagtt tccatccaga
    19321 taaatcagtc cttgcggcaa ataaaatgcc ccctgccagc tgcgcaaagg atctgccgca
    19381 tcctctcttt cacaagcagt tctggattca agtaaataag agatgttgat cggcatggaa
    19441 ggaatttgag ctggggattt atgaattcaa atgcttttct ttgttgaatt ctatagaccc
    19501 tcagtctgta tgtcgagtaa atattttggt ttgttcggca ggttaaatta tccttgatgt
    19561 aaaattccga gaaaaatcag accgtctcga aacagcataa tcgcgaatcc aattaatgca
    19621 aaaccaagga attgcatgat gcgccgatac aatatgccgt gcaggaaatt tcttgatttt
    19681 cctgctgcaa ttgcgagcaa aagtttgact ccacacaagc ctaaataaaa actgaaaagg
    19741 aaagttattc cggaacccca agtctgttga aatgagcgaa ccatcagagg tgctcccaca
    19801 gtaaaccaaa aaaggtatgg attgggatta actaaatttg caatcattaa ttctttcagg
    19861 agtagcgggc ggggtgtaaa atccgtaatt tctgcattgg tggttcgaat actatttgtg
    19921 cccaaatata gtagaaaaac tgatccaaac agagaaattg cacccagcag gatattcata
    19981 tttgaaattt gggcgaataa gaatccggaa attaatacga cagggatatc tgtcagtaaa
    20041 ggaatacaag ccacttttgc accggcgcgg atgccgtgac gtaatgtttc agaaatgata
    20101 agtatcaaca ttggcccggg agcaattccg gacggagcac ccaggagcaa tccaatagta
    20161 aagattcccc aaatactatc gatcatgcag gaatctcaaa atccagacac tctaaatcaa
    20221 gggtcatttt gccgattcta gtgaccgagc ctttcataat gatattataa ttttcgctga
    20281 ttgtgatgtc gatttgccct cccggcatta agaccgcgat ttcagcatcg cacaagccca
    20341 gccgatgagc cactgatgcc gccgcacaac tgctgcttcc tgatgccaaa gtgtaacctg
    20401 caccacgttc ccagatttcg atccggatcg tgttgcggtt cagtacttcc aaaaactgca
    20461 catttgtacg gtttggaaag ctcggatgcg tttcaatcaa aggcccgagc cgtctggttt
    20521 cgtctgccga tatttttcgc tgcagcacca cacagtgcgg attaccgatg gtggcggagc
    20581 agaatgttaa tttttcaccg tttatttcca atgtttcatt caacacttcc cgactttccc
    20641 ccgtgaccgg aataatgttg ctttgaaaac ttacagagcc catttcgaca gttacggact
    20701 gaccggaact gtctacctgt gcgcagacaa ttcctccgga tgtaacgatt ttaaattttg
    20761 actgatcaac cagtttccgg tcccagagcg aacgcgcaaa aatgcgtaaa ccgtttccgc
    20821 ttttttcagc cgtgctgccg tccggattat atattttaag tgtgaagcag tttccggagt
    20881 cgtcttttcc tcccactaat ataccgtctg aaccaagtcc aaagttgcgg tgacatattt
    20941 tacgaacttg ttctgatgtc agttcatttt ccgaaaagtc cggatgttca ctggttgata
    21001 ccaggtaatc attgccatga gcatgatatt tgagaaattc cattttttta ggttaagatg
    21061 ataacattat aataatttag gccttaaata gttaaactat tatgcccgga gattactttt
    21121 ggtaaaacca cattctgaat ctaattttgc taactttatt caataatcag ctattttctg
    21181 agcggcttct gtcggagtaa gcggtttgtc taaaagctgg ccgttactca gccgcaataa
    21241 aacgccttct tcagtcatgt aaacttgcat agggtcaccg ttttcatcca gcatttccag
    21301 ggcatatgtc gattttccgt ccagaaaatc cggaattaat cttacatttt ccggggcgtt
    21361 gtcgcctatt tctctccaaa gtgattctac atccttgtat tgctggtcca gctgccgtag
    21421 ctttttttgc aaatcttggg gcgaagcaaa tttttccagt ttgcctttcc aaaagagaaa
    21481 caatgatttt gcttcagaaa aaacaaatac tgtattttta cctatttccc ggaggatatt
    21541 attcgcaata ttttccaaaa tttctggcga tgcagtgacc atttttttca ggcgctcact
    21601 ggattcttga acagggatgt tagaaccaaa tgactgcatc atatcttcca aattaaactc
    21661 agacaattta tttgcagtag aactctttgc agtcaaagtt ttattttttg gcacaaattt
    21721 taaatgtttc attaaacttt tccccggagg ctgtccctga ttctggaaaa aaaatattag
    21781 aaacgtgccc aggaaaataa acaatataat cagaataatt ttccgtaaaa attttcccgc
    21841 agatttattt tcatttttgc tcccagcggc agatctttcc ctgtgtttaa aagagtgttt
    21901 ggttttttcg agcattttcg cagaaattat cggctcaggg aagtatggct gcaactcctg
    21961 cattagttga tcatttcgtt cctgaaccag ttttactctt ttaattaact caaaaccgtg
    22021 tgttttgagg tagattttcc ggagctcttc cacaagaaat tttacgccag gatgggtagc
    22081 ttctttaatt tcttccggat cttgcttcgt tccattccta aatcgctggc attgatgaag
    22141 gacttctact tcaagtgctt ttgaacctac acgcaaggca tgccagcggt cccgcggaaa
    22201 cataggcggc gaaaccggcg gtgttatatt ttcttcattt gaactattat ttgcagagtc
    22261 tacagatagt gtttttgccg atttatgctt acttttacgt acctgacttt ttttcatttg
    22321 gaatatttgc cagtcttttt tgtaatttga ccatgcctgc ttgaattcat cccaattatt
    22381 gcgtaactct ttattatgct taaataattt attaaaaagt ggtgcaatat ctggaaaaga
    22441 aagtggatct tttttgcgga cgagaattga caatttccgc atcttttcca tttgcttctc
    22501 cagtagcacg gactctttgt cgatatgatt ccattcagta tttgtcaatg ggcgtttcca
    22561 atgttcaaca actggaaaaa taagcaattc cattctgatt gtatctacac aaattcgatt
    22621 cagatcgatc gatttggtac ttgggacatg tgtttgagtt tgctgttgca atgcatgcca
    22681 ggcttgcttt gttttagtgg atttgtgatc cggatttgtg aggagcataa aatcctcaaa
    22741 atggtcacct gagatgggag taatgtctga tttgttcatg ttttttatat agacgaatta
    22801 tgttccaacg aatataatgt gtttagcatt gcaaaactcg tactttagtg taatccacta
    22861 tcaaaaccgt ggtcgatcag ggaataagtt atgtttgact tacaaattaa attacagtag
    22921 aatatcctaa tcagtaccaa tttggtgctg tttctctaat tacttggttg aatcatgccg
    22981 ttacttgagt attgcacttt gaatcaccat gcattcacat ttaattttca aattatttaa
    23041 cagggttagg ttatgaaatt taaagttgtt tctccaaatg tagaaagttc agggaattcc
    23101 ggaactgatc cgaaggctca gattgagcaa atgctttcgg gcagtcctgt ttttttattt
    23161 atgaagggta cgcccgaatc acctcaatgc ggattttctt ccaaaatagc agatatttta
    23221 aaggcctggg aagtccctta tcagtcattt aacgtacttt ctgacgaaag tattcggcag
    23281 ggagtcaagg actttgcaaa ctggcagacc atcccccaac tttacatcaa caaagaattt
    23341 gtcggtggat cagatgtagt tgaagaaatg tcgaataacg gtgagctggg tgatttgttg
    23401 aaagaagcgt ttcctggtcg agacatcaca ccaccccctc ctccggtaga agtacaggaa
    23461 gtagcagccc ttgaagctgc ttcaattctg aaagaaaatc ctgaaattcg tcttttggat
    23521 gtgcgcacgc agcatgagcg ggagactgca tctctggata attctgtgtt gctggatcag
    23581 gaattagtgg aggagatcct tggttcgtgg gatcaaaaca caccactgat gtttttttgc
    23641 cacatgggag aacgcagcag acaagcagct caatatttta cctcacaggg ttttcaacag
    23701 gtgtacaaca tctctgacgg aataaagggc tggtcaagca atgtggactc ctcaattcct
    23761 caatactaga attcataatt tttttactta gaaagctcca gggttatgaa aacagaaaaa
    23821 tcacttccat cagtcgttct aacagagcgt gctgcacaag tttttaaaga atcttgtgaa
    23881 gcagaaggct caactttgga ggatacattt ttacgcattg gagcacgtcc gggtgggtgt
    23941 tccggatgga aatatgagct tgaatcaaat acctcggaag atattgggcc aaatgatgtg
    24001 caaattctgt caagagaagt ttctgtaatt gtagacaggg aagcactaac tgaaatctta
    24061 gggccactaa agattgattt ttcagacaaa aatatggttg aacaggggtt tgtctttgaa
    24121 caactgacaa ccggacacca gtgcggatgc ggagaatctt tcactcccgt aaaagatttg
    24181 aataccgtaa aataaaatgt tccagtttct tgaatggctg gctggggcaa cctgcttcgg
    24241 gctgtttatt tacaagtgga ttatcattgc agcagtggta atgacctggg tcagcgctga
    24301 tccgcataat cagattgttc agtggatcgg acgtgtaact cgtccattat gggtctggtg
    24361 tgaacagcgg atgccggtga tgatggcaca tttcagtgcc tatgcctcaa ttcttgttgt
    24421 tatctttgcc cagattgtat taccggctga aatacgctca ctgaatttgt tgtttcaggg
    24481 gcagacggat tttgctggtg tagtgcttca gagtggaggg cacttgctgc agggtatgag
    24541 tattgtagtt caaagccttt tgtttttttg catattcgta ttgattgcct ggtttgtact
    24601 gactttggta aacccgtctt taagtaatcc gttggtacgc atcatccatg tacttgccga
    24661 tcctctgatt actcctgttc aaagttatct tccaagaacc cgtaccgatt ggtcaccatt
    24721 ggttagtgta ctgttatttt atctgatcag ttcaagcata atttcaccaa taggatttta
    24781 cgggagcacg ctttcaaatc cggtctctat ctgcatttat tagatgatac ttttaacatt
    24841 acatatagtc gccattggag ccgtgattgg tgtaactgca aactttgctt attctatggg
    24901 taaaaaagaa ggcctccggg tcgctcgcaa agagcacgag gataaggaac taaaaaaaga
    24961 agtaccaaaa atcgaatcct aatagaattt atatcagagt ttttttgcag cttgttgttt
    25021 tttcaaacgc actttaattg cttcagcatg ggcaaacaat tgttcttctt ctgcaaaacg
    25081 aattatttgt tctccctgac gcacaagacg ctcaggagaa tagctgatga cagaactggt
    25141 ttttacaaag tcctgtactc caagtggtga tgaaaacttt gcactacccg aagtgggcaa
    25201 ggtgtgattc gggccagcaa aataatctcc gactggttcg ggagaatttg gtccaagaaa
    25261 tatggctcct gcattgcgaa tacggtgcag atcttcaaaa ggttgttttg tcaaaacttc
    25321 caaatgttcc ggggcaagct cattaatcac ttcaaaaatt tcttctaaat cttctgccac
    25381 aattattgcc gagcggtttg caaaagatgc ttctatgatt tcacggcgcg gaagtgtagg
    25441 taccaatttt ttaagacgtt tagatacatc ttttgcctgt tttgctgatg ttgtaacgag
    25501 aacggctcca gcatcaggat cgtgttcggc ctgggacagc atgtcccgga caaggtattc
    25561 aaccggaata tcgtcacggt cacaaacaac catgatttca cttggtcctg caaatgagtc
    25621 tataccgaca gttccgaaaa cctgccgttt tgcttctgca acatacgcat ttcccggtcc
    25681 ggtaattttg acaacttgct gaatcgattc tgtaccatag gccaatgctc caatagattg
    25741 tgcaccgcct actgaataaa gttcttcgag acccatcaat gctgcacttg ccatcactaa
    25801 ttggtgcgga agtccggaag gtccaggtgg agacaccatt gcaattcgag gcactcctgc
    25861 aacctgggcc gggatcacgt tcataagcaa agtcgaagga taagctgcac gacctccggg
    25921 gatataaatt ccgacactat ccactggtgt gactttccaa cccagcacag tgccgtctgg
    25981 actgaaatcc agttggcttt ctgttttttg ccgctcgtga aaaaacatga tattgtctgc
    26041 agcttcttcc aaaatgtgaa acagctcgtg atccaggttt tcgagagctt ctgtcagtaa
    26101 aggttttggt actagccacg attcgggaat cacgttatcg aaccgttcag tatattcacg
    26161 caaagccgaa tccccttttt ccctgacggt ttccaatatt tcctgaactg taacggaaac
    26221 gtctttattt tctttagcat cgaaggattt tgaaaagaga tcaaatcctt caaatatttt
    26281 aacctgcatt tttttctctg tagtaagtac aattttgtga cggttcgttt actgtatcaa
    26341 tcatggaaag aaacaatttt cgttcagaaa caacacttga gatatgttgt tctatttagg
    26401 caattaagct ctctgcttct ttaaattcaa cggaaacagt tttggaaata ccgggctctt
    26461 ccattgtaac accgtacaaa aggtcgccga tttccatagt ctttttattg tgggtgacaa
    26521 ttatgaactg agaatcgtaa gataaacctg taataagccg gttgaagcgc actacattca
    26581 catcatccaa aggtgcgtca acttcgtcca gtaaacagaa tgggcttggt ttaaccaaaa
    26641 aaatcgcaaa aatcagtgag atagcagtca gggctttttc tcctccggaa agcaaattga
    26701 tgttttgtaa ttttttaccg ggaggttgcg ccactatatc aactcctgca ttcaacaaat
    26761 cttcagattc tgtcagtact agcttagctt ctcctccttc aaacaaagaa ctgaatacat
    26821 tcatgaaatt ctcattgacc tgatcaaaca tttctctaaa tcgacgtcgg gactcaatgt
    26881 tgatgtcttt gatggtttct tttaaatcat ctacggcttt ttgcaaatct tcggactgtg
    26941 atttcaaaaa atcaatcctt tctttcagcg ccgcatattc ttccggtgca gcaaggttta
    27001 ctgcccccat tgcattcaaa cttgccttta atgaacgtaa ttccagtccc atttgtttat
    27061 gatcggaagt atcaatatca atttctgtaa gtatagcttc aggagtttgc tcggtaattt
    27121 ctctcaattg atcttcaagc tgttcccttt gcagtctgaa ttcagtcaac cgaattgatt
    27181 cctgatgtga aacgctcact gaattttcaa gatgaccctg acgttcctgc aatttttgag
    27241 agagcagagt ctgctcttca tttttctgtt cgtgcagttg aatatcttct tccaactccc
    27301 gcttcatgtc ggaacgtttc tccagcaagt cctcaaaaga ttcatcaatt tcagtaattc
    27361 gtttacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac gtttctccag
    27481 caagtcctca aaagattcat caatttcagt aattcgttta cgactttgtt cgagcctcag
    27541 atctccctca ttaagtgcag ttttaagctg ttcaagtcgc agagaggttt cttcatgttc
    27601 tctgttcaag cgactttctg tttcctcagt gtttttttgc tgctcagttg tctcagtcaa
    27661 agaaactcga tgggcaagca gttcctcagc aattgcatcg ttgcgacttt gcagcagttg
    27721 tatgcgttcc tgcagctgat ttgttttttc ttccagttca gtacgttctt tttccagtga
    27781 aataatagtt tctgcggcag ttttttcttt caatcgtgat aagtccattt cttcaagtat
    27841 gctgtctgta tcttgagaaa gttgcgaaac tgtctgctcc attcggcgct cctccaactg
    27901 gtggtgttcc agttccttgt tgccgctcag cagagaaaat tccaattttc gtgattcttc
    27961 ttctcgggac tcctgctcct ctttcaaaga ttcatgtttt tcccggagag taagcaattc
    28021 actctcaaac ttttttatct ttttttgtaa atcatcaaca tatgttccaa catcaatgat
    28081 ttgctgctga cgctgcaaaa aacccagaga tccggattgt acttttccca tgctggcgga
    28141 agaatttgaa agacaaattc cggatgagga gagccactct tcaggagagt ccggccactt
    28201 tttatcagct tcattccaaa attttttttc atctttcagc agtgcaaatc gtgaaaaaaa
    28261 cttttctccc cactcttgta aaggtttctt aaacttgaga atttccggaa gaggaatacc
    28321 tttggttcct gctgatttcg gaacagatgc aggacgatct aaagctacaa acccgagttg
    28381 ccccaattcg tgttcagcac aaaatatttc tatttgcgga agcttttctg ctcgttcaat
    28441 aaccacccag tccagcactt cagccattac aggagatacg atatctaatg tatccgcagg
    28501 cacagtaata aaatcggcaa gcagtcctga cactcccagt tgcttcttag tttccggatt
    28561 gtcctgcatc aactgcatga aaatttttac actgtcacta aatccttcat agtgagtttg
    28621 gatttgctgc agggactcaa tacgagattc tgcagtgctt tgctgataac gcagatcctc
    28681 aagttttttc tcctcttcaa ttagtttcaa gtgctgatct tctatgctac tttcaagttc
    28741 agctaatttt cccgacaatt cttctttctg caagcgcatg gtcaccatct gttcttctct
    28801 gtctgaaacc tggagagtta cttcctgaag cagatttttg tgagattgtt gctggtcttg
    28861 aagacgttgc tgtcgttctg taagattttc caggcgttca tccaggaaat tcttctgatt
    28921 agtttggtta gttaattcag tgtgtacagt taacagtcgt ttttgaagta cagaagtttc
    28981 atcggtggat ttttggagag tttcgttttc ttcagcacgc ttctcttcga taataacaag
    29041 ttttgcctgc atttctccca aacgttctgc cagttccatg ctccggttat ttatatcagc
    29101 tctttctttt tcacgttcag ttaatctttg tttcaactct tcgagctctt tttcctgacc
    29161 ctccctttgt tcactgacat tttgcagatt ttgtttttcc agttcacgtt gattttcagc
    29221 ttcctgtata ttttgctctg ttttaaacag ttgttcgcgc cgctctccaa cctgtgtacc
    29281 caattgtgtt tgctctaact taagccgttc taaatcagtt tcaacaactg ccttttcacc
    29341 ttgaagatcc tgctgctgct gctgctgccc ctttaccagt tcttgtactt tttgttcgtt
    29401 gagacctgct tgatgccaac ttaaacaaag atgctgacgc tccagcaggc cgctctgttc
    29461 ctgtagctcc aaatattttt ctgcccgttc gacatgttcc cgcaacccgt cttcctgttc
    29521 cgaaagcgct ccaagcacgt cgttcacccg aagcagattt tgtgttgttt catccagccg
    29581 tttttcagca ttttcacgtt tgaagcgaaa cttgacgatt cctgcagcat catcaatcaa
    29641 gatgcgtcga tcaataggtt tggcgctgac aatttcctct acgcgcccct gttcaacaat
    29701 cgagtatccg gttccgccaa ttcctgtgtc cataaacaat tcccgcacat ctttcaaacg
    29761 cacgggtatt ttgttgatca tgtaaatact ttcacctgaa cggtaaaggc ggcgggtcac
    29821 cgcaatttct gtgtaatctg caaattgttg aagtgtgtct cctttggggt tggaaaaagt
    29881 cagtgagact tcagccattc cggaaggctt gtgaaaggag cttccggaaa aaattacatc
    29941 ctccattgaa ctgccgcgta aatgttttac acgctgttca ccaagtaccc agcgaactgc
    30001 gtcaacaaca ttgctttttc cgcagccatt tggacccact ataattgtaa ttccgttttg
    30061 tttaacctgc agattgacag gatgacaaaa tgatttgaat cctgaaactc ttattttttt
    30121 tatacgcaca aattttttct attttctttt cagctttttt tacaaacaag acacttaaaa
    30181 ttaaaattac ttatctcaga atcagagcta aatgcacttt atttatcagg catttaagat
    30241 tttagctgca aaccgttcaa ctctttccga catagtttcc agttaggata aaactgatcc
    30301 attaatgcct tgaatctgtt gttgtgactg gcttctagca aatgcactaa ctcatgtaca
    30361 acaatatatt ccagacattc aggtgacttt tttacaagct caagattgaa acgaatacca
    30421 tgggttttag gcgtgcagct gccccacctg gatttcatgc tgcgaatgga gaagcgcttg
    30481 actgagacac ccagtttaag ttcccatttt tttataagag gattaagtag ctccatcaac
    30541 tgtttatgat accactcatt caggacagaa cgcctttttt ccatgtcagc tccagtagga
    30601 acatgaatca atatttcgtt tttcactagc tctgcaaaag gaggtttgtt attttccaac
    30661 acttttaatt gatagctgtg acctctaaag tagtgagttt cctgatttat atatttcaaa
    30721 ggtttttcac gcacctgatt tcgtattttt aaacgctgtt ttctgatcca atcaagcttg
    30781 gatagcgcga aaacacgaat tttttcctga ttcatacgga gtggtgcaga aacacgcacg
    30841 ataccgttag gagggcatac cttcaagtgg agatgtttga ttttcttttt agttacctct
    30901 gcatcgatat catccagcat aatcattgaa atcatacgtt tttgtttaac tttcacagcg
    30961 cgttgctttt ctgtaaacaa agagagtaca ttaatgcttt caaacattgt aacaatggtt
    31021 ggcgggttaa gtgaaaactt cttgaactgt atggctggtt tcttttgtca aatagttttc
    31081 attcgtgtaa aggagcatag caccagttaa aaaattttct gaccttatat attgtggaaa
    31141 cttccgggga atgaaaaata tatggaataa ttagagactc cgcaaacaca ccccacatga
    31201 cactttgcaa actttcaggc atttgaaata atttttatat ctaaaaaggg atatcatcct
    31261 tgatgaaacc gtcatcatgt ggagtttcct ggacgttccc tggatcctgt gttgatgcag
    31321 aatcctgtgt tgatgcagaa gttgcagaat aatttgaaga gcctccttcc tgaggaattc
    31381 ccccatcaac attttttgaa tccagaaact gcatgtttgt gactacaacc tcagttttgt
    31441 agtgtttttg accttgttga tcttcccagt ctctagtttg taaacggcct tcaacataga
    31501 cttgcgagcc ttttttcaag taagactgtg ccaaatcagc aagtcttccc cacaacacga
    31561 ggctgtgcca ttctgttcgc tcttgttttt caccctgttt gtctgtccaa taatcgttag
    31621 ttgcaatggt ggtattagct actgcactgc cggattgtgt gtagcgaatt tcagggtcct
    31681 gtcctaatcg tccaatcaac atcactttgt tcagatttgc catgttttct cctgaatatt
    31741 agtatttcta agatactctg ttttaacgca atggagtcaa taaggaaagt gcttttttca
    31801 gtctaccttc aaacggcaaa tccgaactgc cggtttctcg aagtactggc aggatttcct
    31861 tttccgaata gccaaggttt tttaatgcag aaaaaactcc gtcaatttca gttcctgaaa
    31921 gagttaatcc tgctttagag ctccctgcag gtcctggttc aaatcctgaa attggatgcc
    31981 gcttcagttg atctttcaaa tccagacaca actttgctgc agtttttttt cctataccgg
    32041 gaacctggct gaaaatctcg gtgctgctct gatgaacagc atccaccagt tgactgggag
    32101 aaagtactga aagaaccgct aatcccaact tcggacctac acctgaagtt ttgataagca
    32161 tttcaaataa ttctttttct tctaattttt gaaagccata cagggaaata tcgtcttccc
    32221 tgacatttgt atagatgtgc agagtgataa attcttccgg agaggcttta acagcaagat
    32281 gttgagaaac atgaactgcg taacccacgc cgtcagatgt tttgagaacg attaaatttg
    32341 cttctgaacg gaggatctgt ccttcaagat aggcaatcat gagataagtt attcataaaa
    32401 atgttcctgc tcaggcagtt catcaagcta aagtgattgt ttaagggaga gtcccaaatg
    32461 agcatgtgtg atagcaacgg caagtgcatc tgcttcatct tcttgaagtg agttctgtaa
    32521 attcaacaga atcttgacca tatgctgtac ttgatccttg tcagccctcc ccactccaac
    32581 tacagtttgc ttgaccttca gagtctgata ttcatgtatg ggtaaatggc gttcagaaaa
    32641 tttcaataaa ataacaccac gtgcatgacc taaagtaagt gctgactgag cattctttgc
    32701 aaaaaagact ttttcgacag ctccacaatc cggtctgaat tcttcaatca attgttccag
    32761 ttctctggag agcattgaca aacgatcaga aagtggatgc tttgcattca gtcggataca
    32821 gccgctggct aaatgtctgg tacggtttcc ggtgttttca atcagaccgt aacctgtttt
    32881 cctgctcccg gggtctatac ctaaaattcg cagtgtttca accaagttct gccataacgc
    32941 tttcgctgat gtcaaaattg gaatggacac tgtttacgtc atcatcttct tccaatagat
    33001 caatcagttt tattacttgt tcagcttttt tataattgtc gatttctata cggttttgag
    33061 gattccagat aacctctgca cttagcatgg caacaccatt ttcttccaag tatgaacgta
    33121 cagtatcaaa atcagcagga gcagtaataa tctgatacgc gtctgggttt tcagtgttta
    33181 aatcttctgc accagcttcc agaattaatt cagtaagccg gtcttcgtca ataacagtgt
    33241 tgtcaaccaa aattgatccc tgtcggtcaa acatgtactg aacagagtta gctgaaccca
    33301 gattcccatt gcatatactg aaagcatgtc ttactgatgc aatggatctg ttcctgttgt
    33361 cagtcatcac ttctacaata attgcaacat tgtgtggtcc atagccttca taaataattt
    33421 cttcaaaaat tactccgtct aattctcctg cacctttttt gatagcccta tcaatattgt
    33481 ctttgggcat gctttgactt tttgcattgg caactgctgt acgtaaacgt ggattggcat
    33541 cgagatcgct tccgccaatt cttgctgcta tagttagttc tttaattatt tttgtaaaag
    33601 ctttaccacg ttttgcatct tgagctgcct tgcgatgctt gattgtactc catttgctgt
    33661 gtcctgacat ataaattcag atttaaaagt ttgaaaaggc tttgattcac aaactactgt
    33721 aaatataata acgcagacta cactaaatca gacaatgcgt ccaattaaat cataagtgtg
    33781 ggattccgta atttcaacat t
//
LOCUS       DTVD01000052            2809 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14635_length_2809_cov_263.380901, whole genome
            shotgun sequence.
ACCESSION   DTVD01000052 DTVD01000000
VERSION     DTVD01000052.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_2809_cov_263.380901"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            91..1701
                     /locus_tag="EYO68_02040"
     CDS             91..1701
                     /locus_tag="EYO68_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC06614.1"
                     /translation="MNLRDNNIERISNKTFDVLIIGGGINGAAAATALAARGAKVALV
                     DKGDFAGFTSQNSSNLVWGGIKYMETYEFPLVRQLCMSRNHLLRSYPSSIREIRFFTN
                     LDKGFRFSPFLLYCGALLYWAIGNFFTRSPRFLTPQQINDDESTVNTDHSIGGFEYSD
                     AFLYDNDARFVFNFIRSAINYNCTAVNYMESLGSNCNTQGEWQTQLRNTRNGETYEVR
                     SAVIINACGPYVDQQNKISELTTEHRHVFSKGIHLIVPQITNNKRVLTFFADDGRLFF
                     AIPMGNRTMVGTTDTRVSTPETEVTTEDRHFVLDNINKRLNLAKPLTEVDIISERCGV
                     RPLVIKTGDQNNKNEEWMKLSRKHAIDVDWERKHISIFGGKLTDCLNVGEEICEVVQQ
                     LGTHLPYPKRKWYGEPHEAIRDEYLHQVHLMGLDELTSAGASEVLSKRLWRRYGAQAI
                     GLLENIRENPDMVEPLIEGTDYIRCELTQAARREMIVTLEDFLRRRSKLALVARHEDL
                     KNSQGLKDACEILFGEEAPERWSEYFGE"
     gene            2130..2387
                     /locus_tag="EYO68_02045"
     CDS             2130..2387
                     /locus_tag="EYO68_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06615.1"
                     /translation="MESKTKVVTSYVSHEDVQSAMERFLDHGGEITKIDDPCQEILLR
                     SDMNEEDMNYIDPHAANPSRNGLGQVGNVLQQKVQAELKQA"
     gene            2384..2758
                     /locus_tag="EYO68_02050"
     CDS             2384..2758
                     /locus_tag="EYO68_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIC06616.1"
                     /translation="MNIGKAVVFVLLALFLVGALPLFISPEHSESAQRKGFSIEDRLI
                     PLEEILGGGPPRDGIPSIDSPKFILAAEADWLFPDSRIIGLDIEGDIRVYPLAILNWH
                     EIVNDTVGGVPVSITFCPLCGT"
BASE COUNT          816 a          579 c          604 g          810 t
ORIGIN      
        1 atttcagtcc atgaagtgca aaaaacgtat aataatcaac tatcttttta ttaataatta
       61 tatagtctta gtaatttttt gaaatatttt atgaacctaa gagataacaa tattgaaaga
      121 atatctaata aaacattcga tgtcctgatc atcggtggag gcattaacgg tgcagctgcc
      181 gcaactgccc ttgccgcacg aggcgctaaa gtggctttgg ttgacaaagg tgattttgcc
      241 ggtttcacca gccaaaattc ctctaatctc gtctggggtg gtatcaaata tatggaaact
      301 tacgaatttc ccttggtgcg acaactctgc atgtcacgca accatcttct ccgttcttac
      361 ccttcgtcca tccgggagat ccgatttttt accaatctgg acaaaggatt ccgtttttca
      421 ccatttttgc tttattgcgg agccttgctt tattgggcaa taggtaattt ttttacacgt
      481 tccccacgtt ttttgactcc gcagcaaatc aatgacgacg agtccacggt taacaccgat
      541 cacagtatcg gcggctttga atattctgat gcgtttctct acgacaatga cgcacgcttt
      601 gttttcaatt ttatacgttc tgctattaac tataactgca ccgcagtcaa ttacatggaa
      661 tcattgggca gcaactgtaa tacgcaaggt gaatggcaaa cacaactccg gaatactaga
      721 aatggagaaa catatgaagt tcggagcgct gtcattatca atgcgtgcgg accttatgtt
      781 gatcaacaaa ataagatttc tgaactgact accgaacatc gtcatgtatt ttccaaaggc
      841 attcatttaa tcgttccaca aattacaaat aataaaaggg tactgacttt ttttgcggat
      901 gacggtcgtt tattttttgc cattccgatg ggaaaccgca cgatggttgg aacaaccgat
      961 actcgtgttt ccactccgga aacagaagtt actacggaag atcggcattt tgttctggac
     1021 aacattaaca aacgtctgaa tctagccaaa ccactgactg aagtagatat tatttccgaa
     1081 cgctgcgggg tgcgccccct ggtgatcaaa accggtgatc aaaataacaa aaatgaagaa
     1141 tggatgaagt tgtctcgtaa acacgcaatt gatgtagatt gggagcgaaa acacatcagt
     1201 atttttggag gaaaacttac ggactgcctc aatgttggtg aagaaatatg cgaggtcgtt
     1261 caacaattag gaactcatct tccttatcca aaacgaaagt ggtacggaga acctcatgaa
     1321 gccattagag atgagtatct tcatcaagta catttaatgg ggctggatga attgacctct
     1381 gctggagcct cggaagtgtt gtcaaaacgt ctttggaggc gctacggagc gcaagccatc
     1441 ggactcctgg aaaacatccg ggagaatccg gacatggtag aaccattgat tgaaggaacc
     1501 gattacatcc gctgcgaatt gacccaggcc gcgcggcgtg aaatgattgt gactttagaa
     1561 gattttttgc gacgccgctc taaattagct ttagttgccc gccacgaaga ccttaaaaat
     1621 tctcaaggtc tcaaagatgc ctgcgaaatt ctgtttggcg aagaagctcc ggaacgctgg
     1681 tcggaatatt ttggtgaata gccgtaaagt aggtaaatat ttctggacgc tctttttttg
     1741 tgctccgaaa cttcattttt gttaaagtca aaaaacagca attttgagac agagcaggga
     1801 aatttagatc tgcagtaaag tcaaaaaatc aacgattcga cttcctccac aactacatag
     1861 ttcactgata aaacaacata tatttaactt gtgccgtctg actcaaatat agtttttaga
     1921 ttcccggact tggttgaata gatatttttc ataatgtaat ttaatttatt cttcacgatt
     1981 cttgcattac cttttttgta ctaaatgtag cggtttaatt tcgacagtcc gtttagtttt
     2041 tggaacaggt gtaagatttt ctccggaaac tcgtcaaatt aagtagaccg ttgcatttct
     2101 ttcacttcca agttaataaa ggagatgcta tggaaagcaa gacaaaggta gtgacatcct
     2161 acgttagcca tgaagatgtt cagtctgcta tggaacggtt cttagatcac ggtggggaaa
     2221 ttaccaaaat cgacgaccct tgccaggaaa ttttgctcag gagtgatatg aacgaagaag
     2281 acatgaatta cattgatccg catgccgcaa atccatccag gaacggtttg ggtcaagtag
     2341 gcaatgttct ccagcaaaag gtgcaagcgg aactcaagca agcatgaaca ttgggaaggc
     2401 agtagtcttc gtactactgg ctttgttttt agtaggggca ttgcccctct tcatcagtcc
     2461 ggaacattca gaatcggccc agcgcaaggg attttcaatt gaagatcgct tgatccctct
     2521 ggaagagata ctgggcggcg ggccgccgcg tgatggtatt ccttctattg attcccctaa
     2581 atttatttta gccgcagagg cagattggct gtttccagac agccggatca ttggcctgga
     2641 tatcgaagga gatatccggg tctatccgct ggccatttta aactggcacg agattgtcaa
     2701 cgacactgtc gggggagttc ctgtttcaat cacattttgc cccctctgtg gaacctagta
     2761 tcggcatttt acttaaaaaa taatcatgaa tgaaattaat ttaaaacca
//
LOCUS       DTVD01000053            2798 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14709_length_2798_cov_412.961721, whole genome
            shotgun sequence.
ACCESSION   DTVD01000053 DTVD01000000
VERSION     DTVD01000053.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14709_length_2798_cov_412.961721"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..450)
                     /locus_tag="EYO68_02055"
     CDS             complement(1..450)
                     /locus_tag="EYO68_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06617.1"
                     /translation="MIRKCSLMFSLIALTTFLSVQNPHAKPVGNRFTTSVTLGPAVFP
                     MPGIRFQYRFNDHLSLNSGLSYIVAAGDFNAGINFHLPVNSIDPYLGARRIFIYHLTG
                     SIDLSAGVAGIETENYFVEAGLGFGQERTDFGSTKTELAVLQIGWFF"
     gene            complement(582..2120)
                     /locus_tag="EYO68_02060"
     CDS             complement(582..2120)
                     /locus_tag="EYO68_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC06618.1"
                     /translation="MSKLDFLKNLGLDKQNSGVSTGTEWLTGNGSLIESVSPADGSVI
                     AHVRNANREDYETVISLAQDSFQEWRKIPAPQRGEIVRQIGNAVREAKDDLGKLVSWE
                     MGKIYQEGLGEVQEMIDICDFAVGLSRQLYGFTMQSERPGHRMYDQYHPLGIVGIITA
                     FNFPVAVWSWNAMIAAVCGDVSIWKPSPKTPMCSIALQHIIGKVMRENKMPEGVFNLV
                     IGTNEEIAETLVNDTRIPLISATGSTNMGRNVSAKVASRLGRSILELGGNNAIIVTES
                     ADLKIAIPGIVFGSVGTCGQRCTTTRRIIIHDSIYDDVKNQLVQAYSQLENRVGDPLD
                     SDTLIGPMIDEVAVKTFRNALKAIREQGGEIIYGGDILDGYPSELYVRPALAEVENHW
                     PIVQEETFAPLLYLIRCQDFDEALRLQNDVPQGLSSAVFTRDFQESEKFLSHEGSDCG
                     LANVNIGTSGAEIGGAFGGEKDTGGGRESGSDAWKAYMRRQTNTINYSSDLPLAQGVN
                     FDLG"
     gene            complement(2165..2584)
                     /locus_tag="EYO68_02065"
     CDS             complement(2165..2584)
                     /locus_tag="EYO68_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HIC06619.1"
                     /translation="MRYLSLILFSGFLVFSVDFATQNTEIVALHYSLDWLNFSYQSER
                     PVFVPVFFSFAFGIIFSVIYFFIYHAFLLKNLRQQKKEIKRLKNLVESGRVKQGSMEE
                     RNSELQQIVQRVQNRVDGQSDLEPQTLLDDKAVKTSP"
BASE COUNT          784 a          637 c          583 g          794 t
ORIGIN      
        1 tcaaaaaaac catcctatct gtaagactgc caactctgtt tttgtactgc caaaatctgt
       61 tctttcctga ccaaatccaa gacctgcttc cacaaagtag ttttctgttt caattccggc
      121 aacacctgcg cttaaatcaa ttgagccggt caagtgatag atgaatattc tccttgcgcc
      181 aagataaggg tcaatagaat tcacaggcag gtgaaaattg attcccgcgt taaagtctcc
      241 tgctgccact atgtaggaca aaccagaatt gagtgaaaga tgatcattga acctgtattg
      301 aaagcggata cccggcattg ggaataccgc cggaccgagt gtgactgagg tcgtaaagcg
      361 attaccaacc ggcttggcgt gagggttttg gactgacagg aaagttgtca atgcaatcaa
      421 actaaacatc aaagagcatt ttctgatcat ggaaaacctc ttgtattttc tggattctct
      481 ttccaatctt tgcagcagac tcagacgtgt tttaggaaaa gaacgttgaa tgaatatttc
      541 gaatttgttt tttagattac aattcggttc aattttgatt attaacccaa atcaaaatta
      601 acaccttgtg ccaacggcaa gtcggaagag tagttgatag tgttggtttg acggcgcatg
      661 taggctttcc aggcatctga tccggattcg cgccctcctc cggtatcttt ttccccgccg
      721 aacgccccgc ctatttctgc tccggaagtt ccgatgttga cgttggccag tccacagtca
      781 gaaccttcat gagaaagaaa tttttccgac tcctgaaaat ctctagtgaa gaccgcggat
      841 gacaaacctt gaggaacgtc gttttgtaaa cgcagagctt cgtcaaaatc ctggcagcga
      901 atcaggtaaa gcaatggcgc aaaggtttct tcctgcacaa tcggccagtg gttttccact
      961 tccgccagcg caggacgcac ataaagttcc gaggggtatc catctaaaat atctccacca
     1021 taaataattt ccccaccttg ttcccggatt gctttaagag cattccggaa ggttttcacg
     1081 gccacttcat cgatcatggg gccgattagt gtatccgaat ccagtggatc accaacccta
     1141 ttttcaagtt gggagtatgc ttgaactagc tgatttttta catcatcgta aattgaatca
     1201 tggatgatga tccgacgtgt ggttgtacag cgctgaccac aggttccaac cgaaccaaaa
     1261 acgattcccg gaattgcgat cttcaaatcc gcactttccg ttacaataat agcgttgttt
     1321 cctccaagtt ctaaaatact gcgtcccagt cgtgaagcga ctttagctga aacattacgg
     1381 cccatgtttg tggaaccagt agcggaaatc agtggaattc gtgtatcgtt gaccaaagtt
     1441 tcagcgattt cttcatttgt gccgatgacc agattaaaca caccttcggg cattttgttt
     1501 tcacgcatca ccttaccgat tatgtgttgc aacgcaattg aacacattgg tgttttagga
     1561 ctcggtttcc aaattgaaac gtctccacaa actgctgcga tcatggcgtt ccatgaccaa
     1621 accgcgaccg ggaagttgaa cgcagtgatg attcctacaa ttccaagagg atggtattgg
     1681 tcatacatgc ggtgaccggg tcgctcagac tgcatggtga aaccataaag ctggcgtgaa
     1741 aggccgactg cgaaatcgca aatatcgatc atttcctgca cctctcccaa accttcctga
     1801 taaattttcc ccatttccca tgagaccaat ttcccaagat catcctttgc ttcacgtacc
     1861 gcatttccaa tctgacgcac gatttctcca cgctgcggtg ctggtatttt gcgccattcc
     1921 tgaaaagaat cttgtgccag agaaattaca gtttcgtaat cctcacggtt ggcgtttcgg
     1981 acatgtgcta tcacggaacc gtctgccgga gaaacagatt cgatcaagga accgtttccg
     2041 gtaagccatt ctgtgcctgt acttactccg gaattctgct tatccaaacc aaggtttttc
     2101 aggaaatcaa gtttgctcat aatatctttc aaatcttaat taaaattcaa ctatagtttc
     2161 ccattcaggg agaagttttt acagccttat catccagaag tgtctgtggt tccaagtcgg
     2221 actgaccatc tactctgttc tgaacacgct gaacaatctg ctgcaattcg ctgttacgct
     2281 cctccattga accttgtttt acccgccctg actcaactaa gtttttgagt cgtttgattt
     2341 ccttcttttg ttggcgtaga ttttttagta aaaatgcatg gtagatgaaa aaatagatga
     2401 cagaaaaaat tatgccgaat gcaaaggaga agaaaactgg tacgaaaaca gggcgctcac
     2461 tttggtaact gaaatttaac cagtctaaac tatagtgtag ggctacaatt tcagtatttt
     2521 gtgttgcaaa atcaacactg aagacaagaa aacctgaaaa taatatcagt gacaaataac
     2581 gcataataaa taaggtcaag aaagcttgtg catgaataaa aatcttcagc cagtatctat
     2641 cacaaattac tttggtgtca agtcaagtgc tgtttttatg tgaagaactg tcttaggaga
     2701 agtatggaca atttggatga attttggcat aataaagcta tgtcatctgt acggagatgg
     2761 atgcgtcact ggtgggcgcc ccggacttca aacccgcg
//
LOCUS       DTVD01000054            2798 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14710_length_2798_cov_296.168429, whole genome
            shotgun sequence.
ACCESSION   DTVD01000054 DTVD01000000
VERSION     DTVD01000054.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_2798_cov_296.168429"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(312..1166)
                     /locus_tag="EYO68_02070"
     CDS             complement(312..1166)
                     /locus_tag="EYO68_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06620.1"
                     /translation="MSSLPPYLSIPERIWHYTFWVICGAVLFFLIFPLLVITPLSFNA
                     VPFFTFTKEMLAFDPAGYSLVWYEEFFTSLNWQGAIRNSVIIAFFSTIIATFLGTLAS
                     LGLSRPNMPYRTLLMSLLISPMIVPLIIAAAGMFFFYSRLGLQGTHLGVILAHAVLGT
                     PFVVITVTATLTGFDNNLIRASQSLGASPTTTFFKVIVPLITPGVISGALFAFVTSFD
                     EVVVVLFVGSYRQRTIPWQMFSGIREQISPTILAAATLLIIVTIALLTTLELLRRRTE
                     RLRGVTPS"
     gene            complement(1169..2438)
                     /locus_tag="EYO68_02075"
                     /pseudo
     CDS             complement(1169..2438)
                     /locus_tag="EYO68_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2629..>2798)
                     /locus_tag="EYO68_02080"
     CDS             complement(2629..>2798)
                     /locus_tag="EYO68_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC06621.1"
                     /translation="SSAPLVGKHATLGIEMAPHMPTDPNNAKTTLLFNYEFWADHRDD
                     LDARFNAWLLK"
BASE COUNT          936 a          567 c          563 g          732 t
ORIGIN      
        1 caggaaaacc tcgaacaggg ctatccatca tttttgcctc cttttatgat ctggttaaat
       61 gtttcggtat cagtaactcc ttcggaacca attagcaaaa cacgggaagt ttcatctaag
      121 ccaagtttca tgcgcaattc ctgttgtttg gaacagcaga taagtactgc aagacctgca
      181 acagcacttt ctccagcctt gataacaggg tcattgcctt cataggaaac attgcttcaa
      241 tggaagcatt aaataaggga acttatttta aaatattctg aaactaaaat atatttattg
      301 aagtttctta attaggaagg cgtaacaccc cgtaggcgtt ctgtacgacg gcgcaacaat
      361 tcgagtgtag ttaaaagcgc aatagtgacg ataatcaata aagttgctgc cgcaagaatt
      421 gtggggctta tttgttcgcg aattccggaa aacatttgcc atggtatcgt gcgttgtcta
      481 tatgaaccaa cgaaaagtac aaccactact tcatcaaaag aagtaacaaa agcaaaaaga
      541 gctccggaaa taactcctgg agtaatcaaa ggtacaatga ctttgaaaaa agttgtcgtg
      601 ggcgaggctc ctaaactttg agaggcacga atcaagttat tgtcaaatcc agttaaggta
      661 gcggtaacag tgataacaac gaatggtgta ccgagtactg catgagctaa aatcacacct
      721 aaatgagtcc cctgcaagcc aagacggctg taaaaaaaga acatcccggc agcagctata
      781 atgagaggaa caatcattgg tgaaatcaaa agactcatca ataaagtccg atatggcata
      841 tttggacggg aaagtcctaa tgaagccaaa gttcccagga aagttgcaat gattgtagaa
      901 aaaaaggcaa tgatgactga gttacggatg gctccctgcc agttcaagct ggtaaaaaac
      961 tcttcgtacc aaactagaga atagcctgca ggatcaaaag ccagcatttc cttcgtgaaa
     1021 gtaaaaaacg gaaccgcatt aaaagaaagt ggtgtaatta caagcagcgg aaaaatcaaa
     1081 aaaaacagca cggcaccgca tatcacccaa aatgtatagt gccagattct ttccggaata
     1141 cttaaatatg gaggcaaaga actcataatt aacctaactt catgttgtca attccaacga
     1201 ttcgatcata gagcataaat aaggctaata cgattcccag taaaataact cctaatgctg
     1261 atcccaatcc ccagttaaga gaacttgaaa tatgataagc aatcctgtta ctgataaatg
     1321 tccctgaact tcctcccacc aatgccggag taatgtagta accaattgca atgatgaaaa
     1381 ctaaaattga acctgcacct atgccaggac cagtattggg gaaataaact ttccaaaaag
     1441 ccttccatgg atgcgccccc agggaaaccg ctgcacgtac ataagatttc ggaattgttt
     1501 tcattacact gaacaaaggt aaaatcataa aaggcaacag aatgtgcgtc atggctacca
     1561 cagtaccagt agcattatgg atcatcgcca accgaccgtc attgttgatt actcccacta
     1621 ataccagaaa atcattgatt accccttctt gctgtaaaag agcaatccag gaagtggtac
     1681 gcacaaggag tgaggtccaa aatggcagga gtacaaaaat aattagaata ttactcaaac
     1741 gtgtagacac tgtggccagc atataagcca aagggaaacc aatgataaaa gtcaaaaatg
     1801 taatcagaga actcaaaagc attgttctcc aaaaaagttt tagataaatg ctttctttgc
     1861 ctggaagaga ttcaactttg cctgttgtag gatttcgttt aaaatccaat gcagcaaggt
     1921 agtaaccatc agtgtattcg gttgaaaatt gcttaattac ctgccatgta tatatttgcc
     1981 cccagcgttt atcagattta ataagggctt ctttgtaggg gggggttttt gatttttttc
     2041 acttttcgtc cagctttccg gaacaagctg gaaaatccag agctttcata gttcaggcgt
     2101 tgcccgactc tagtatgttt tcttgcgatt tttgcttttt taatttcttc aaccattgca
     2161 gcaaatacta cttcatccgg aagttctcca gaagcttcat cccagttttg taaagctatt
     2221 actgattttg gcagaatttc ttgtgtaatt gagttgtcca ctgatagcga cagcatatca
     2281 gcaattggaa tcagaaaggc aatcaggata aaaatcagca gaggtaaaac cattgagaat
     2341 gcaacaattt tactacgacg ttgtgccctc gccaagctaa tcttgagcgg agtaccgtcg
     2401 gcggtaacta aaacagattt ttcttgggga atttccatca aataggtaag aaaacaaagt
     2461 aaaccgattc agcttctcat ttaaagatag ctgcaaatac tgtcgtcaaa ttaattttat
     2521 gcacctattt aaatgagtag cgtaatccaa caaattgaga atgaacaagc cgggttacgc
     2581 taacgccaac ccggcctacg aaagattcgt atttgtccaa ctggatgatt acttcaacaa
     2641 ccaagcgtta aacctggcat caagatcatc acgatggtct gcccagaact catagttgaa
     2701 aagcagagtg gtttttgcat tgttgggatc agtcggcata tgcggagcca tttcgattcc
     2761 aagagttgcg tgttttccta caagaggtgc ggaagacg
//
LOCUS       DTVD01000055            2775 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_14909_length_2775_cov_216.157721, whole genome
            shotgun sequence.
ACCESSION   DTVD01000055 DTVD01000000
VERSION     DTVD01000055.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14909_length_2775_cov_216.157721"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(249..1253)
                     /locus_tag="EYO68_02085"
     CDS             complement(249..1253)
                     /locus_tag="EYO68_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC06622.1"
                     /translation="MKTLETMGKRLVRRSEMVPCKSAFIDAHTPGSHLKDNFSIIGPG
                     VTENKEQFINIREPHGFNIGAAGQPPHIKNSLHSHFTAEVFMIHEGAFEIYWGLKAEN
                     RTTLSEGDVVSIPKNCFRGFENIGDKYGFMFAILGGDDTGGVEWAPQVFEEAEDHGLV
                     LLEDYGVWDTNKHPIPKGAKPVRPMSHEKAATYENYSTEEMELRICRSDSLKPLKNHP
                     LKLGEYGSIQLHRLIGTKEAVIPGGDGFELLLSVAKPGGGFKTFCRPEKEVLICYEGI
                     WKVDWTGEGQKGSFLLNAGDLFSVPDHFGRSMECVGKNEGSLYSVINENSPKDPSWTN
                     "
     gene            complement(1365..2507)
                     /locus_tag="EYO68_02090"
     CDS             complement(1365..2507)
                     /locus_tag="EYO68_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HIC06623.1"
                     /translation="MKLSKDRILTTHVGSLPRSEKVFKLVFAKEDGKNLDKKEYDEVI
                     ADAVKTVVKKQEEVGIDVVSDGEQSKISYATYIKDRLNGFEGDSPRRTPKDLEEFPTF
                     SSRASKSGGVPTYTRPRCTGPVTVKDMKPLEIDLGNLRKALDGISISEAFMNAASPGV
                     IALFQPNEYYPDHKSYLYALADSMKAEYEGIAKAGFLIQIDSPDLALGRHVLFKDKSD
                     EKFLSMIQLHIEALNHALENISSEKLRLHVCWGNYQGPHHCDIEMKKILPTVLKAKPQ
                     ALSFEGSNPRHSHEWTVFRDIKIPEEKILIPGVLDSTTNFVEHPELVSERICRFADIV
                     GRNRVIAGSDCGFSTFAGFGTVDADITYAKLSSLAKGAEIASKKLW"
BASE COUNT          779 a          553 c          515 g          928 t
ORIGIN      
        1 tgatattgat agagattatg agcgcattat tagaatcacg tattgaacgt gaagggaagc
       61 ccagactcga ctatattgaa caggcaaagg aaattgtaag cgggttaaat tgaataaggt
      121 ttcttcatct accttccaaa atattgtccc acagttagat tagaagtaaa atgtttcaca
      181 tcaaaattta gtatgaatta attatgggct aagtgtagcc cgcaaaaaat agacaaaggg
      241 tgatacaatc agttagtcca actaggatct ttcggtgagt tttcatttat gacagagtat
      301 agggatccct catttttccc aacacattcc attgaccttc caaagtgatc aggtactgaa
      361 aacaaatcac cggcattgag caaaaaactt cctttttgcc cctcaccagt ccaatcgact
      421 ttccagattc cttcataaca gattaggact tctttttcag gtctgcagaa cgttttaaat
      481 cctcctcctg gtttggcgac ggagagtaat aattcgaatc catctccacc aggaataaca
      541 gcttcttttg ttcctattaa acgatgtaat tgaattgagc cgtattctcc aagttttaaa
      601 ggatgattct ttaatggttt tagagagtct gagcggcata ttcgtagctc catctcttca
      661 gtagagtagt tctcataagt tgcggctttt tcatgactca taggtctaac tggttttgca
      721 ccttttggaa tgggatgctt attggtatcc catactccat aatcttcaag caatactagt
      781 ccatgatctt cagcttcttc gaatacttga ggagcccatt caacaccgcc tgtatcatct
      841 cctccaagaa ttgcgaacat gaatccatat ttatcaccta tgttttcaaa tccgcgaaaa
      901 caattttttg gtatagaaac tacgtctccc tcagacagag ttgtacggtt ttcagccttc
      961 aacccccagt aaatttcgaa agctccttca tgaatcatga atacctcagc tgtgaagtgg
     1021 ctatgtagcg agtttttgat gtgaggtggc tgacccgctg ctccgatgtt gaatccatgt
     1081 ggttcgcgta tattgatgaa ctgttcttta ttttctgtta cacctggccc aataatacta
     1141 aagttgtcct tgagatgact ccctggagta tgagcatcaa taaaggcact tttacaggga
     1201 accatttcac tcctacgaac tagtcttttt cccatagttt caagtgtttt catagatgac
     1261 acaaaaattt attatttttt ggtgaagaaa acagtttcaa aaatttgtca agcggtctct
     1321 gttgggtgtt tttgttaata atatcacacc atgctggaaa gattttacca aagctttttt
     1381 gaagctattt ctgcaccttt tgccagcgat gaaagttttg cataggttat gtcagcatcg
     1441 actgttccaa aaccggcgaa tgtagaaaat ccacagtcgg agccagcaat taccctattt
     1501 cttccaacaa tatctgcaaa tcgacaaatt cgttctgaaa ccagttcagg gtgctctaca
     1561 aagttggttg tcgaatcgag tacacctgga atcaaaatct tctcctcagg aattttgata
     1621 tctctgaaaa cagtccattc gtgactgtgt cgtgggttag atccttcaaa tgaaagagcc
     1681 tgaggtttgg cttttaggac tgtaggaaga attttcttca tctcgatatc acagtggtga
     1741 ggaccctggt agttacccca acagacatgc agacgaagtt tttctgagga tatgttctca
     1801 agtgcatgat ttagggcttc gatgtggagt tgaatcatag aaaggaactt ttcgtcactt
     1861 ttgtccttga aaagtacatg acgtccaaga gctaaatcag ggctatcaat ctgaattaga
     1921 aagcccgctt tcgcaatccc ttcatattcg gctttcattg aatctgctaa agcatagagg
     1981 tagcttttgt gatcgggata atattcattt ggctggaata gagcgatcac acctggtgaa
     2041 gcagcgttca tgaaagcttc agagatacta atcccatcaa gtgcctttcg taaatttccg
     2101 agatcgattt caagtggttt catgtccttt actgtaactg gcccagtaca acgtggacgg
     2161 gtgtatgttg gaacaccacc ggattttgat gctctcgagg aaaatgttgg gaattcttca
     2221 agatcttttg gagtgcgtcg aggactgtca ccctcgaatc cattcagacg gtctttgata
     2281 tatgtggcat aactgatctt actttgctct ccatcactta caacatctat tcctacttcc
     2341 tcttgttttt ttaccactgt tttaacagcg tcagcaataa cttcgtcata ttcttttttg
     2401 tctagatttt ttccatcctc ctttgcgaag acaagtttaa aaactttttc agaacgagga
     2461 aggctgccaa catgtgtggt cagtattcta tctttactta atttcatatt tatttttctc
     2521 ctttcttgtg tgcttatcta atcgctcgtc tgaagaatac tattttatat cggcgtatca
     2581 aactttttat caaatatttt ataatgaatt ccattgcata cgtatgttat gttgatattt
     2641 tcttaataaa tttaccagtg aaatatcatt tttattcatt ccataattaa taattactgt
     2701 tttatttaaa aacaatcaaa tgtttgaatt tgattaaaac ttgactcaaa gtacttggat
     2761 gatgtagtta tgcat
//
LOCUS       DTVD01000056           10873 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1499_length_10873_cov_448.258828, whole genome
            shotgun sequence.
ACCESSION   DTVD01000056 DTVD01000000
VERSION     DTVD01000056.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_10873_cov_448.258828"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            6..938
                     /locus_tag="EYO68_02095"
     CDS             6..938
                     /locus_tag="EYO68_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HIC06624.1"
                     /translation="MNSRIYVAGHRGLVGSAIYRLLKKRGFENLITRTHSELELMDAV
                     AVQNFFEETKPEFVFLAAAKVGGIHANSTYPADFMRENLVVQTNVIHESWRNGVEKLM
                     FLGSSCIYPKLCPQPISEESLLTGELEPTNEAYALAKIAGIKTCQSYNQQYGTNFISA
                     MPTNLYGINDNFHPENSHVLPALIRRFHEAKLADSESISIWGTGNPRREFLHSDDLAD
                     AVLFLMENYNDSEIVNVGCGEDQTIRVLAETISEVVGYSGSLAFDSSRPDGTPQKILD
                     ISKIRVLGWTPEIPLKKGLEQVYQWYTEQESSAT"
     gene            complement(945..2327)
                     /gene="hemN"
                     /locus_tag="EYO68_02100"
     CDS             complement(945..2327)
                     /gene="hemN"
                     /locus_tag="EYO68_02100"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:PRK09249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIC06625.1"
                     /translation="MTADLLAVTPEIIQRYNRPGPRYTSYPTVPVWSEGYFSEDYAAC
                     LQKEGLNEQPISLYLHIPFCRQLCTFCGCNKYITNNQEIVERYLDALDQEIAGVAKLL
                     GVRKKIAQVHFGGGTPTFLNHSQLRRVVEMIHSCFDVQEDGEWALEADPRVTTNKQLE
                     LLYELGFRRISFGVQDLDPAIQQAINRNQTVEQSWRTLETARNLGYKSINMDLIYGLP
                     LQTRQSFRKTLEEVYKMRPDRLAIYSFAYLPGMFKTHKRAIREKDLPSPEEKIAIYLE
                     TIRFLAEAGYVMIGMDHYALPTDELALAAKNRTLHRNFMGYTTLHNKTQIGVGVSAIS
                     DFGDSYWQNTKEIDQYMNEATEKKLIPRKGMTLDEEDLLRRDLIETLMCHGEISITDF
                     ETRYKIEFQDHFSQAWKKLEQFKQEGLVNLSLEKIELTPVGILFTRNLAMPFDRYLEE
                     SAHQHFSKTV"
     gene            complement(2412..3206)
                     /locus_tag="EYO68_02105"
     CDS             complement(2412..3206)
                     /locus_tag="EYO68_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06626.1"
                     /translation="MLDDFFWRAVIAGMGVAILAGPLGCFVIWRRMSYLGDTMAHSAL
                     LGVALGIFLEVDLIFGVFVVAIAVAMILFIFQRQKQLANDAVLGTLSHASLAMGVLAI
                     SLMSWVHVDLMGYLFGDILAVDMTDLYWIYGGGLLIMLIFLWLWRPLLALTFDSELAQ
                     AEGVPVIRVQLIFMLLIAAVIALSMKIIGILLVTSLLIIPVSAARRFSRTPEQMALGG
                     ALIGMASVGLGLWASLKIDTPSGPSIVIAAVLFFFAANLFPLRKFQ"
     gene            complement(3209..4003)
                     /locus_tag="EYO68_02110"
     CDS             complement(3209..4003)
                     /locus_tag="EYO68_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIC06627.1"
                     /translation="MPTKIETAANFSTAKEKKQILLQTENMSLFAGEQELLSGVSLTV
                     SAGEIVTIIGPNGAGKTTLFRVLLGVIPPNTGSIYKKPNLRIGYLPQKITVDPILPLS
                     VSRIMNLTGNFSVRQIENVLDETGVLSLKNKPVYQLSGGEFQRVMLARTLLINPELLV
                     LDEPVQGVDYMGESELYKLIGNLRNKHGCGVLMVSHDLHVVMASTDRVLCLNRHVCCE
                     GEPEDVSQHPEYLRLFGLDSGSALAVYSHHHDHTHQLSGTLREDRP"
     gene            4100..5047
                     /locus_tag="EYO68_02115"
     CDS             4100..5047
                     /locus_tag="EYO68_02115"
                     /inference="COORDINATES: protein motif:HMM:PF01297.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HIC06628.1"
                     /translation="MKTGICFIIVIPLLWFNLAMAASDLKVVVSIKPFHSLVSTVMQG
                     VSEPALLLNGNNSPHTYSLRPSAAVKLQNADLVFWGGENLEGFLAKAIHSLAAGARVV
                     SFEDTPGLILRPFRSVKEWQELDPESKNDQDHIKKHEIHRLPGNDPHIWLDPLNAQKI
                     TQYLVQILSEFDPENAQTYHSNGKKTILRLSDLNIQLETKMSSVSSKSYIVFHDAYQY
                     FEKRYQLNPIASVTVSSGPSTSVGRLIDIRKKIKNKNVLCIFTEPQFSPKLVQTVISG
                     TAVKKGILDPIGTSISPGPEMYFTLLNNISHSISNCFKK"
     gene            5089..9954
                     /locus_tag="EYO68_02120"
     CDS             5089..9954
                     /locus_tag="EYO68_02120"
                     /inference="COORDINATES: protein motif:HMM:PF05088.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06629.1"
                     /translation="MSKIKKIREENTLVQDDSDNSVFDQFITHFLRGMSERMRETYSN
                     SELEVFLKDRFAFFRKAVSRSGMVQVVPHKLPSNTHEESYFAGNVIIEIVSPDAPFIV
                     VTVEALLRQLDLMIHRKLHPIMGVRLTAEKEILGVISPVDTSEKFDHLYLEIDADADI
                     VSLKRIENLIAGHMLAVQLVQNQRQNMLKRLESMEKEIDTVTVISSDTKEEWSKLCGW
                     LKLDNFTLMGFITLVAQDSDSTNFIKTIDNSGLGILAETFLAKSSKTLLNVVTARLSK
                     RRHSEFPFALDLIRFTSPVLRFENLMMLSIRVPDKKSGMVEHVFLGLLRGSSLQVKNV
                     ETPLIHQKMDYIFKNRNMLVNSYDYNEVVRIFRATPKFELFRSSRKELIQVCDGLLSV
                     NNPNNIYCFQINTRVTGVLKLMIVMPTSLFSEEAIGHTVVLVKAKIPHQHCDWFEARG
                     SEKSRLHLEFELQEDTLGKSELPNVDLLQLENEISGLIKPWKIQLRELLQSKNTGDEG
                     ARLYERYVPLMPSHYSARVEILEALENIQFMELLTRDDDLQFDLKHFSIPSVLGKSIS
                     LLYVYSKEKIHLIEIMPVLQNLGLHVIDQLTTRIGNDEKTLAFIQSFRVVRSDRRKIE
                     EEHFKPLLAPIVKQVFKKKTENDPLNGLALLANLAWREINVLQLYRNLSLQLSAPLTS
                     ETINGVLLRHPLCSRLLFETFACRFSPESSFGNLIYRQEVLLPQKKHEFIESLVTVKQ
                     VTDDEVLRRLFELIENTLRTNYYLQQDTEETGISIKLDSRKIEQMPDPVPFKEIYVHD
                     VGMEGLHLRFGPVARGGLRWSDRPDDFRTEILGLVKTQQTKNVVIVPVGSKGGFVLKN
                     TPASREEAITESKNQYRRFISAMLQITDNFDAQGKIQTPSHVLSYDDPDPYLVVAADK
                     GTATFSDIANEVSEKCDYWLGDAFASGGSVGYDHKREGITARGGWECVKLHFKEMGRD
                     IQAEHTTVIGIGDMSGDVFGNGMLQSKMLLLKAAFNHMHIILDPDPDAESSWQERKRL
                     FDLPGSSWMDYSTERISSGGGIYERNAKSIKLSPEIKGMLGTDADSLKGEDAVRLILQ
                     MDVDLLWFGGIGTYIKTADQTHYQVGDQANNSLRIDTTECQMKVIGEGANLGLTQLAR
                     IDLANNGVRLNTDAVDNSGGVNMSDYEVNLKILLQQLLRRGIVGSKEERNDLLASATD
                     EVSELVLANNRGQHRLISMDSIRSNLNFRLFRKLIAHLQEQGMNKRGEYIPTRTELDQ
                     LEHANMPLPRPVLSVLMAYAKMEIYEALTSSEMPLEKELTATYLEYVPKTLKSHFGEN
                     ANDHPLKKEIVSTVLTNNITNQAGSTFVSRMAQVTERSIPDIIRTYLILESSLGATEI
                     RERLYSMTDISEKERYEVLIDLEDVLKMLVRNVLQSQAVPPGFEQIDQYHGLLSELVE
                     HPDNSVDTLNSENDQSPPDSEREKVQSEDLSEQRAIDAVRTSLRRLRLAPDVIHLCIN
                     KKLGVSTAYRIAESVEQKFGLDWLRERLILQEPGNDWELEFQDILLRTLDANKLRLLE
                     VLLASHKIETMKEQDFPTLLEPLEEMNAAHLRAYIQSLEQLKSGSLISLTSIAVNLSH
                     LDFLKNISSKI"
     gene            9987..>10873
                     /gene="cpsB"
                     /locus_tag="EYO68_02125"
     CDS             9987..>10873
                     /gene="cpsB"
                     /locus_tag="EYO68_02125"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: protein motif:HMM:PF00483.21"
                     /note="capsular polysaccharide colanic acid biosynthesis
                     protein; catalyzes the formation of GDP-mannose from GTP
                     and alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIC06630.1"
                     /translation="MIAMILAGGTGTRLWPYSRSMTPKQFLNLGSTHESLFQETCRRL
                     ETLIDPEKIYVVGSASHEGELQQQMAQIYPDYRPEQLLIEPLSRNTAPAILWGILQIP
                     ENQRGEPVVILASDHLIKAPEHFIGALKNAETLASSGYLVTFGIRPDRPETGYGYIKA
                     GEALEVGFKVADFVEKPDQSTAESYLESSDYTWNASIFMATAETWLDEFRNHAPGLLA
                     VFENATVDGKKLADPEVIRKIYQSIESDSIDYALLEKSKRVAVLPVDMEWSDLGSWES
                     IYQVSEKDKQGNVIRGNVIS"
BASE COUNT         3258 a         2179 c         2338 g         3098 t
ORIGIN      
        1 tgaaaattaa ttctcgcatt tatgttgcag gacaccgtgg tctggttggt tctgcaattt
       61 acaggttact caaaaagaga ggatttgaaa acctcattac ccgcactcat tccgaactgg
      121 aactgatgga tgcagtggca gttcagaatt tttttgagga aacaaaacct gaatttgttt
      181 ttctcgctgc agcaaaagta ggaggtattc acgcaaacag tacttaccct gctgatttta
      241 tgcgtgaaaa tttagtcgtt caaacgaatg ttattcacga atcatggagg aatggtgtag
      301 aaaaattaat gtttctaggc agttcatgca tctatcctaa actatgtcct caaccaatta
      361 gtgaggagtc tttactgact ggtgaattgg aaccaactaa tgaggcatat gctctcgcaa
      421 aaattgctgg tatcaaaacc tgtcaaagtt acaatcagca gtacggtaca aattttattt
      481 ctgcaatgcc aaccaatctt tatggaatca acgacaactt tcatccggaa aactcacacg
      541 tcctgccggc cttgatacgg agatttcatg aagcaaaact cgccgattca gagtcgatca
      601 gcatctgggg gacaggaaat ccaaggcgtg aatttttgca ttcagatgat cttgcagatg
      661 cggttttgtt tctgatggaa aattataatg attctgaaat agtcaacgtc ggctgcggtg
      721 aagatcaaac gatcagggtt ttggcagaaa cgatcagtga ggtcgttggc tattccgggt
      781 ccttggcatt tgactcgtca cgtccggatg gtactccgca aaagattctg gatatttcca
      841 aaatccgtgt tctgggctgg acaccagaaa ttcctttaaa aaagggactt gaacaagttt
      901 atcagtggta tacagagcag gaatcttctg ctacatgagt attattatac tgttttggaa
      961 aaatgctgat gtgcactttc ttcaagatac ctgtcaaaag gcatggccag gttcctggta
     1021 aatagtattc ccacaggtgt cagctcaatt ttttctaaac ttaagttcac tagtccttcc
     1081 tgtttgaatt gttccagctt tttccaggcc tgagaaaaat gatcctgaaa ttctatttta
     1141 taacgtgttt caaaatccgt aattgaaatt tctccatgac acatcagagt ttcaattaga
     1201 tcacgacgta acagatcttc ttcatccagt gtcattcctt tgcgaggtat gagctttttt
     1261 tctgtggctt cgttcatgta ttgatcaatt tctttcgtgt tctgccagta actgtctcca
     1321 aaatctgaaa ttgcagatac acccacacct atctgagtct tgttgtgcag agttgtgtaa
     1381 cccatgaaat ttcgatgcag agttctgttt tttgctgcaa gagcaagttc atctgtggga
     1441 agcgcgtagt gatccatgcc gatcattaca tatcctgcct cagccaaaaa gcggatagtt
     1501 tccagatata ttgctatttt ctcttcaggt gaaggtaaat ctttctccct gattgcacgc
     1561 ttatgtgttt tgaacattcc gggtaaatag gcaaagctgt aaatcgccag ccgatccggg
     1621 cgcattttat agacttcttc aagggttttt ctaaaactct gtcttgtttg cagcggcagt
     1681 ccgtaaatca gatccatatt tatgctttta taacccagat tgcgggcagt ttccaatgtt
     1741 ctccatgact gttccactgt ctgatttcga ttaattgctt gttgaattgc cggatccaaa
     1801 tcctgtactc caaaactgat cctccggaaa ccaagttcat aaagcagttc cagttgtttg
     1861 ttagtagtta ccctcggatc ggcctccaag gcccattctc catcttcttg tacatcaaag
     1921 caggagtgga tcatctcaac tactcttcgg agttgtgaat ggttaagaaa agtcggagtt
     1981 cctcctccaa aatgaacttg tgctattttt tttcgaacgc ccaggagttt agctacaccg
     2041 gctatttcct ggtcaagagc atcgagatat ctctcgacta tttcctgatt gttggtgata
     2101 tatttattgc agccgcaaaa agtacagagt tggcggcaaa aaggtatatg caggtagaga
     2161 gaaatcggtt gctcattcag cccttctttt tgcaaacagg ctgcataatc ctcggagaaa
     2221 tatccttcgc tccagacagg aacagttgga taactggtat aacgcggtcc cggtctattg
     2281 taacgctgaa tgatctccgg tgtaactgca agcaggtctg ctgtcatttt tgtatagcgg
     2341 atttttatta tttttcttta ctaaagaatg atgccgtttt aaaaccagtg ccaagaatct
     2401 ttaaacaatt tctattgaaa tttacgcaga ggaaaaagat tggctgcgaa aaaaaacagt
     2461 actgcagcaa taacaattga gggaccggaa ggtgtgtcaa tcttcagtga tgcccacaag
     2521 ccaagtccaa ctgaggccat acctataagt gcacctccaa gtgccatttg ttcaggagtt
     2581 ctactgaaac gcctcgctgc agaaacagga attattaaaa gtgaggtgac cagcagaatt
     2641 ccgattattt tcatggataa ggcaatcaca gctgcaatca aaagcatgaa gatcagttgt
     2701 actctgataa ctggaactcc ttctgcctgg gcaagttcac tgtcaaatgt aagagcaagt
     2761 aaaggtcgcc agagccaaag aaatatcagc atgatcaaaa gaccgccgcc atagatccag
     2821 taaagatctg tcatgtctac tgccaaaatg tcaccaaata aatatcccat taaatcaacg
     2881 tgcacccaac tcatcaggct gattgccaac actcccatgg ccagtgatgc atgagaaagt
     2941 gtacctaaaa cagcatcgtt tgcaagctgt ttttgtcgtt gaaaaataaa aagaatcatt
     3001 gctacagcga tggcaacaac aaaaacaccg aaaatcaaat caacttccag aaaaatacca
     3061 agtgcgactc ccagcaaagc tgaatgagcc atggtgtcac ctaagtagga cattcttcgc
     3121 cagatcacga aacaacccag tggacctgca agaatggcaa ctcccatccc ggcaataaca
     3181 gcccgccaaa agaaatcatc taaaatcatc acggtctgtc ctcacgcaat gttccggata
     3241 attgatgtgt atggtcgtga tggtgactat atacagccaa agcacttccg gagtctaaac
     3301 cgaacagccg caagtattcc ggatgctggc tcacatcttc aggttctccc tcgcagcaca
     3361 catggcggtt caagcacaac acacggtctg ttgaagccat tactacatgc agatcatgtg
     3421 aaaccatcag cacaccgcaa ccatgcttat ttctcaggtt tccaattagt ttgtacaatt
     3481 ctgactctcc catgtagtct actccctgta ccggttcatc gagaacaaga agttccggat
     3541 ttatcaaaag tgtacgcgcc agcatcacac gctgaaattc tcctccggaa agctgataca
     3601 caggcttgtt tttcaatgat aaaacaccgg tttcatccag gacattttca atttgtcgaa
     3661 cggaaaaatt tccagtcaaa ttcataattc ggctgactga aagaggaaga atcggatcta
     3721 cagtaatttt ttgcggcagg tagccaattc tgagatttgg ttttttatat atactcccgg
     3781 tatttggagg gatcactccc aacaaaacgc ggaacagtgt ggttttaccg gctccgtttg
     3841 gtccgatgat agtcacaatt tcgccagcag agacagtaag cgaaacaccg gacagcaact
     3901 cctgttctcc agcgaataga gacatatttt cggtttgaag caggatttgt tttttttctt
     3961 ttgcagttga aaaatttgct gcagtttcta tcttggttgg cattgacaaa tgagttagtc
     4021 agagttatcc tgaagtaagc aatatttata acatatttta ctcttcaaag caaagccgca
     4081 aagcaaaaac tggattttga tgaaaactgg aatttgcttt attattgtta ttccacttct
     4141 ctggtttaat ctggcaatgg cagcttcaga tttgaaagtt gtggtttcaa taaaaccgtt
     4201 tcactcactt gtttctacag taatgcaggg ggtttcagaa cctgctttgc ttttaaatgg
     4261 aaacaactca ccacatacct attccctgcg gccaagcgct gccgtcaagc ttcaaaacgc
     4321 agatcttgtt ttttggggag gagaaaatct ggaaggattt ttggccaaag caattcattc
     4381 tctggctgca ggagccaggg tggtttcatt tgaagataca ccgggactca tactgcggcc
     4441 ctttcgttca gtcaaagagt ggcaggaact tgatcctgaa tctaaaaatg atcaagacca
     4501 tataaagaaa catgaaattc acaggttgcc cggaaacgat ccgcacattt ggctggatcc
     4561 gctcaatgct caaaaaatca cccagtattt agtccaaata ttgagtgagt ttgatcctga
     4621 aaatgcgcag acttatcaca gtaacggcaa aaaaacaata ctcagacttt ctgatttaaa
     4681 catacaattg gaaacaaaaa tgagttctgt ttcatcaaag tcttatattg tctttcatga
     4741 tgcatatcaa tattttgaga agcgttatca actcaatcca attgcttcag ttactgtcag
     4801 ttcaggtcct tcaacaagtg ttggcaggtt gatagatatc cgtaaaaaaa taaaaaataa
     4861 aaacgttctt tgtattttca cggaaccgca attttctcca aaattggtgc agactgtcat
     4921 ttcgggaact gctgtaaaaa aaggaattct tgatcctatt ggtaccagca tttctccggg
     4981 tccagagatg tattttactt tactaaataa tatcagccat tcaattagta attgttttaa
     5041 aaaatgatgt acgtggaata aactattttc cattagagag atgatccaat gtccaaaatt
     5101 aagaagataa gagaagaaaa tactttggtg caggatgatt ccgataattc ggtttttgat
     5161 cagtttatca cacatttttt gcgtggtatg tcagaacgaa tgcgtgagac ctacagcaat
     5221 tcagagttgg aagtctttct caaagatcga tttgcttttt tcaggaaagc agtcagcagg
     5281 agtggaatgg tacaagtcgt tcctcataaa ctgccatcta acacacatga agaatcatac
     5341 tttgccggga atgtaataat agaaatagtt tctccagatg caccgtttat cgtcgttact
     5401 gttgaggcac tgttgcgtca gttggattta atgattcatc gaaaacttca tcctataatg
     5461 ggagtacgcc tgactgcaga gaaagagatt ttgggagtta tttctccagt agatacatca
     5521 gaaaaatttg atcatctcta tctggaaatt gatgctgatg cagacatcgt ttccttgaaa
     5581 cgtattgaaa acctgattgc cggtcacatg ctggcagttc agcttgtcca gaatcagcgt
     5641 caaaatatgt tgaagcggct ggaatcaatg gaaaaagaaa ttgatacagt tactgtcatt
     5701 tcctctgata caaaggaaga gtggtcgaaa ctttgtggat ggttgaaact ggacaatttt
     5761 actttaatgg gttttatcac acttgtagcg caggattcag attctacgaa tttcattaaa
     5821 actatagaca attccggact gggaattttg gcagagacct ttctagctaa atcctccaag
     5881 actcttttga atgttgtaac tgcacgtctt agtaagcgcc gtcactcaga attccctttt
     5941 gcattggacc tgattcgttt caccagtccg gttttacgtt ttgaaaactt gatgatgctg
     6001 agtatccgtg ttccggataa gaaatcaggt atggttgaac atgtttttct cggtctgctc
     6061 aggggaagct cactccaagt aaaaaatgtt gaaacgcctc ttattcacca gaagatggac
     6121 tatattttca aaaaccggaa tatgctggtg aacagttatg actacaatga agtcgtccgg
     6181 atattccgtg caacaccaaa atttgaatta ttccgttcat caaggaaaga attgattcag
     6241 gtttgcgatg gactcctttc tgtcaacaat cctaataata tttattgttt tcagataaat
     6301 accagagtta caggagtatt aaagttgatg atagtgatgc ccacttcact tttctctgag
     6361 gaagcgattg gacacactgt tgtcttagtc aaggcaaaaa ttccacatca gcactgtgac
     6421 tggtttgaag cacgcggaag tgaaaaatca cgtctacatc tggaatttga gctgcaggaa
     6481 gacacactgg gaaaaagtga attgcctaat gttgatttgc tgcagcttga aaatgaaatc
     6541 agcggactga taaaaccgtg gaaaattcaa ctgcgcgaac tcctgcagag taaaaataca
     6601 ggtgatgaag gtgcacgatt atatgaacgt tatgtcccac tgatgccttc tcattacagt
     6661 gcccgtgttg aaatattgga agccctggaa aatattcagt tcatggaatt gctgacccgg
     6721 gatgatgatc ttcaattcga cttaaaacac ttctcaattc catcagtact tggcaagtct
     6781 atttccctgc tctatgttta cagcaaagag aaaatacatt tgattgagat catgcctgtc
     6841 ctgcaaaatc ttggactgca tgtaatcgac cagttgacga caaggattgg taatgatgaa
     6901 aaaactttag ctttcatcca gtcttttaga gtagtacgca gtgacaggag aaaaatagaa
     6961 gaagagcatt tcaaaccatt gttggcacca atagttaaac aggtcttcaa gaaaaaaaca
     7021 gaaaatgacc ctttgaatgg tttggcttta ctggcaaatt tagcatggcg ggaaattaat
     7081 gtacttcagc tttatcgcaa tctatctctc caattaagtg cacctttaac cagtgaaacg
     7141 ataaatgggg ttctgctcag gcatcccctc tgctcacgct tgttgtttga gacatttgcc
     7201 tgtcgatttt ctcctgaatc ttcttttggt aatttgattt atcgtcaaga agtgcttttg
     7261 ccgcagaaaa aacatgagtt cattgagagt ttagtaacag taaaacaggt tactgatgat
     7321 gaagtattgc gtcgtttgtt tgaattgatt gaaaatactt tgcgaaccaa ttattacctg
     7381 cagcaagata ctgaagaaac aggtatttcc atcaaacttg attctcgaaa aatagaacag
     7441 atgccggacc cggtgccttt caaagaaata tatgtgcatg atgtggggat ggaaggtctg
     7501 catttacgtt ttggaccagt tgcacggggt ggacttcgct ggtcagatcg gccggatgac
     7561 ttcagaacgg aaatattggg gcttgtcaaa acacaacaaa caaaaaatgt ggtgattgtt
     7621 ccagttggat caaaaggtgg ttttgtgctg aaaaacactc cggcatcacg tgaagaagca
     7681 atcacagaat ctaaaaatca atacagacgt ttcattagtg caatgctgca gataaccgac
     7741 aattttgacg cacagggtaa gatacaaact ccaagtcacg ttctttccta cgatgatccg
     7801 gatccatatc tggttgttgc tgcagacaaa gggacggcga ctttttcaga tatagccaac
     7861 gaagtgtctg aaaaatgcga ttactggctg ggcgacgctt ttgcttcagg cggttcagtc
     7921 ggttatgatc acaaaagaga aggaatcaca gctcgaggag gatgggaatg cgttaaactg
     7981 cactttaagg aaatggggcg ggacattcag gctgaacaca ccacagtaat tggcatagga
     8041 gacatgagtg gagatgtttt tggtaacggg atgctgcaga gtaaaatgct ccttctcaaa
     8101 gctgcattca atcatatgca tatcattctt gatcctgatc cagatgctga aagttcatgg
     8161 caggaacgaa aacgtctatt tgatttgcct ggatccagct ggatggatta ttctacggaa
     8221 agaatcagtt ccggaggcgg aatctatgag cgtaatgcaa aatccattaa gctgtcacct
     8281 gagataaaag gaatgcttgg tacagatgca gacagtttaa aaggtgaaga tgcagtacgg
     8341 cttattttac aaatggacgt agatctgctg tggtttggcg gcataggtac atacattaaa
     8401 actgctgatc aaactcatta tcaagttgga gaccaggcaa acaactcttt acgaattgat
     8461 actactgaat gtcaaatgaa ggttattggt gagggggcta atttaggctt gactcaattg
     8521 gcgcgcattg atcttgctaa caatggagtt agactgaaca ccgatgctgt tgacaattcc
     8581 ggtggtgtaa atatgtcgga ttatgaggtt aatctcaaaa tattactcca gcagcttctt
     8641 agaagaggca ttgtagggtc aaaggaagag cgaaatgatc tgcttgcatc agcaaccgat
     8701 gaggtttctg agcttgtatt agccaataac cgcggacagc accgtttgat ctcaatggac
     8761 agcattcgtt ccaacctcaa tttccgtttg ttccgtaagc tgattgcgca cctgcaggaa
     8821 cagggtatga ataaacgcgg cgaatacatt ccaacacgta cagaacttga tcagttggaa
     8881 catgcaaaca tgcctttgcc gcgtccggtc ctttcagtat tgatggctta tgcaaaaatg
     8941 gagatctacg aagcgcttac ctcttcagaa atgccgcttg aaaaagaact gactgcaacg
     9001 tatttagagt atgttcctaa aactttaaaa agtcactttg gtgaaaatgc caacgatcat
     9061 cctctgaaaa aggaaattgt ttctacggtt ctgacgaaca atatcactaa tcaggcaggt
     9121 tcaacctttg tttccagaat ggcacaagtc actgaacgga gcatacctga tataataagg
     9181 acctatctga tactggagtc aagccttggt gcaacagaaa tacgggagcg tctgtattct
     9241 atgacagata tcagtgagaa agagcgttac gaagtactga tagatttgga agatgttttg
     9301 aagatgcttg ttcgaaatgt actgcagagt caagcagttc caccgggttt tgaacaaatc
     9361 gaccaatatc atggattatt gagtgaactg gtggagcatc cagataattc tgtagataca
     9421 ttaaattcag aaaacgatca atctcctcca gattccgagc gggaaaaagt tcaatcagaa
     9481 gatttaagcg aacaacgtgc aattgacgct gtcaggacct cactgcggcg tttaagatta
     9541 gctccggatg taattcattt gtgcattaac aaaaaactgg gtgtctctac ggcatatcgt
     9601 attgcagaga gtgtagaaca gaaatttgga ctagactggc ttcgagaacg tctgattttg
     9661 caggagccag gcaatgattg ggaactggaa tttcaggaca ttcttttacg tacattggat
     9721 gcaaacaaat tgaggctgtt ggaagtactg cttgcatctc acaaaattga gacaatgaaa
     9781 gaacaggatt tcccaacttt actggaaccg ctggaagaaa tgaatgctgc ccatttacgg
     9841 gcatatattc agtcacttga gcaactgaaa agcggttcat tgatcagtct gacttcaatt
     9901 gcagtaaatc ttagtcattt ggattttctt aaaaacatct cttcaaaaat ctgaaaaacc
     9961 tgacaattaa ctggtataaa aaatatatga ttgcaatgat ccttgccgga ggaacaggaa
    10021 cacgtctctg gccttatagt cgcagtatga ctcccaaaca atttctcaat cttggttcaa
    10081 ctcacgaatc cctgttccag gaaacatgca ggcgtttgga aacacttatt gatccggaga
    10141 aaatctatgt tgtcggttct gcatcacatg aaggagagct tcagcagcag atggcgcaaa
    10201 tatatccgga ctatcgtcct gaacagttgc ttattgaacc gcttagccgc aacactgcac
    10261 cggccatact atgggggatt cttcagattc cggaaaatca acgcggtgag ccggtcgtca
    10321 ttttggcttc ggaccacctg ataaaggcac ctgaacattt tatcggtgcc ttaaaaaatg
    10381 cggaaactct ggcaagttca ggttatcttg tgacttttgg tatacgtccg gaccggccgg
    10441 aaacaggata tggttacatc aaggcaggag aagcacttga agtcggtttt aaggttgcag
    10501 attttgttga aaaacctgac cagtcaacgg cagaaagtta tctggaatcc tcagattata
    10561 cctggaatgc gagtattttt atggcaaccg cagaaacctg gctggatgaa tttcgtaatc
    10621 atgccccggg attgttggct gtgttcgaaa acgcaacggt tgatgggaag aagcttgcgg
    10681 atccggaagt aatccggaaa atataccaat ccatcgagtc tgactcaatt gattacgcac
    10741 tgcttgaaaa atcaaagcgg gttgcggttt tgcctgttga tatggaatgg agtgaccttg
    10801 gcagttggga aagtatttat caggtttcag agaaagacaa acagggtaat gtcatacggg
    10861 gcaacgttat ttc
//
LOCUS       DTVD01000057            2759 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15034_length_2759_cov_299.485577, whole genome
            shotgun sequence.
ACCESSION   DTVD01000057 DTVD01000000
VERSION     DTVD01000057.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15034_length_2759_cov_299.485577"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="EYO68_02130"
     CDS             complement(<1..640)
                     /locus_tag="EYO68_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HIC06631.1"
                     /translation="MALISLRQLLDHAAENSYGVPAFNVNNMEQIQSIMQAAKATDSP
                     VILQASRGARSYAGDIFLRHLFDAAVEMYPDIPICIHQDHGNNFDTCLSAMANNFTSV
                     MMDGSLEEDAKTPASYEYNVGISSKVTEVAHKIGVSVEGELGCLGSLETGEGEKEDGH
                     GFEGKLSQDQLLTDPDQATDFVKKTQVDALAVAIGTSHGAYKFSRKPDGDILA"
     gene            complement(732..2717)
                     /gene="tkt"
                     /locus_tag="EYO68_02135"
     CDS             complement(732..2717)
                     /gene="tkt"
                     /locus_tag="EYO68_02135"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIC06632.1"
                     /translation="MTVSLRHQDMANALRFLAADAVQKANSGHPGMPMGMADVATVLF
                     TRFLNFKASDPDWPDRDRFVLSAGHGSMLLYSLLHLTGYEDFTLDELKNFRQLGSRTA
                     GHPEYGHGAGIETTTGPLGQGIGNAVGMALAERMLAERFGNDIVDHYTYVIAGDGCLM
                     EGISHEAASFAGHLGLGKLIVLFDDNGISIDGSTDLAVNDDQLMRFGACGWDVSFCDG
                     HDPDAISAAINAAIENQDKPSLIACKTMIGFGSPNKAGTAATHGAPLGDDEITATREA
                     LNWPHPVFEIPEAIESAWRRVGSMKSAKMEAWRKRLAESGRKEEFERVISGTLPENWE
                     APLRDFKQKHSTEKTKVATRKASQETLDVLAEVIPELAGGSADLTGSNLTKAKSQIPI
                     TPGDFSGSYIHYGVREHGMAAAMNGIVLHGGFIPYGGTFLIFTDYCRPSIRLSALMKQ
                     RVIYVMTHDSIGLGEDGPTHQPVEHLSSLRAIPNLNVYRPCDIIETAECWAEALTTSS
                     TPSVLSLSRQGLPCLRTEHTDENLSTKGGYVLAGAEGKRDVTLIASGSEVSIAMEAHD
                     QLKAEGVSAAVVSMPCLDLFESQDKDYRNQVIPSGIPVVVVEAGVEQSWGRYLGEDGE
                     FVGMKSFGASAPANDLFEHFGITTKNVVEAAKRITKK"
BASE COUNT          720 a          687 c          567 g          785 t
ORIGIN      
        1 gcgcaagtat atctccatca ggtttcctgc tgaatttgta tgcaccgtga gaagtaccta
       61 tcgctacagc cagtgcgtca acctgtgttt tctttacaaa atcagtagct tgatcaggat
      121 cagtcagtag ttgatcctgc gataatttac cttcaaaacc atgtccgtct tctttttcgc
      181 cttcaccggt ttccaatgaa cccagacagc caagttcacc ttctaccgac acaccaattt
      241 tatgcgccac ctcagtgact tttgaactga tgccaacatt gtattcataa gaagccggtg
      301 ttttagcatc ctcttccagg gaaccgtcca tcattacact ggtgaaatta ttggccattg
      361 cagagagaca agtatcgaag ttatttccgt gatcctggtg tatgcaaatc ggaatgtccg
      421 gatacatttc aactgcggca tcaaacaaat gccgcagaaa aatatcacct gcataggaac
      481 gtgctcccct gctggcctgc aaaattacag gcgaatccgt agctttcgcc gcctgcataa
      541 tcgactgaat ttgctccatg ttgttaacat tgaacgccgg aacgccgtag ctgttttccg
      601 ccgcatgatc aagtaattgt cttaaagata ttaaagccat aatttatact cagatttagg
      661 attaaggaaa agctgccaag ttcgctgata aacgtaaagg gatttcttag tattttcaat
      721 tttcatggcg gttatttttt cgtaattctt tttgctgcct ccactacatt tttcgtagtg
      781 ataccaaaat gttcaaaaag gtcgtttgcc ggtgcggatg ccccaaaact tttcatgcct
      841 acaaattctc cgtcttcccc aagataacgc ccccaacttt gctcaacgcc tgcttccaca
      901 acaactaccg gaattccgga aggaattacc tgatttcggt aatctttgtc ctgagattca
      961 aacaaatcca aacaaggcat ggagactact gcagctgaaa cgccttcagc tttcaattga
     1021 tcatgtgctt ccatggcaat ggaaacttcc gatccagaag cgatcaatgt cacatcccgt
     1081 ttaccttcag ctccggccag gacgtatccc ccttttgtac ttaaattttc gtcagtgtgt
     1141 tcggttcgca ggcatggcag accctggcgg gagagagaaa gcacagaagg tgtggaggat
     1201 gttgtcagtg cttcagccca acattcagca gtttctatta tatcacatgg tctgtacaca
     1261 ttcaaatttg gaatcgcacg taagctggaa aggtgttcca ccggttggtg ggttggtccg
     1321 tcttcaccca aaccaatgga atcatgtgtc atcacgtaaa tcacacgctg tttcatcaag
     1381 gcagacaaac gtattgatgg tcgacaataa tcagtaaaaa tcagaaatgt tccgccatac
     1441 ggaatgaatc caccatgcag aacgatgccg ttcatggcag cagccattcc gtgctcccga
     1501 actccatagt gaatataact tcccgaaaag tcacctggag taattggtat ttgtgattta
     1561 gcctttgtca aatttgatcc ggtcaaatca gcagaaccac cagccaattc cggaattact
     1621 tctgctaaca catccagtgt ttcctgactt gctttacggg tggctacttt tgttttttct
     1681 gtggaatgct tctgcttgaa atcccgtaaa ggagcttccc agttttccgg aagtgttccg
     1741 gaaattacgc gttcaaattc ttcctttcgt ccggactcag ctaaacgttt acgccaggct
     1801 tccattttag cacttttcat tgacccgacc ctgcgccacg cactttcgat tgcttcagga
     1861 atctcaaaaa caggatgtgg ccagttaagc gcttctcgtg tagccgtaat ttcatcatca
     1921 ccaagtggtg ctccgtgtgt agcggcagtt ccggctttgt ttggtgatcc aaaaccaatc
     1981 atcgttttgc aggcaatcag tgacggcttg tcttgatttt ctattgcagc gttgatggcc
     2041 gcagaaattg catccggatc atgtccgtcg cagaatgaga cgtcccatcc gcaagctcca
     2101 aaacgcatca gttgatcatc gttgacagca agatcggtgg atccgtcaat ggaaattcca
     2161 ttgtcatcaa atagcactat caattttccc agacccaaat gtcctgcgaa tgaggctgct
     2221 tcgtgactta ttccttccat caagcatccg tctccggcaa tgacataggt gtaatgatct
     2281 actatgtcgt ttccaaatct ttcagcgagc attcgttcag caagcgccat tccaacagca
     2341 ttcccaatcc cttgtccaag cggtccggtc gtggtttcaa ttcctgcacc gtgtccatat
     2401 tccggatgtc cggctgttct tgaaccaagt tgtcgaaaat ttttcagttc gtccagcgta
     2461 aaatcctcgt aacctgtcaa atgaagcagt gaataaagca gcattgaacc atgccctgca
     2521 gacaagacaa agcggtcccg gtcaggccaa tccggatcac ttgctttaaa atttaagaat
     2581 cgggtgaaca atacggttgc tacatccgcc atgcccatcg gcattcctgg gtgacctgag
     2641 tttgcttttt gtacagcatc cgctgctaaa aaacggagtg cgtttgccat gtcttgatgg
     2701 cgaagagaaa cagtcatagt gaaatcaatt agctataaat aatttataca aaagttgtc
//
LOCUS       DTVD01000058           10821 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1516_length_10821_cov_394.129946, whole genome
            shotgun sequence.
ACCESSION   DTVD01000058 DTVD01000000
VERSION     DTVD01000058.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_10821_cov_394.129946"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(40..1446)
                     /locus_tag="EYO68_02140"
     CDS             complement(40..1446)
                     /locus_tag="EYO68_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIC06633.1"
                     /translation="MNIDSINYTLILYTAFCILLGIFFCWFYQKKMISTQSQEIDALG
                     ESLNELLKKYDTILADKIELDKQFAILKQDANRIPEIEEELKFQQKQFSTLQVLNATL
                     EERLESKENESREKLKFLEEAKKLMGTEFENLSAKIFTSKSKQFVEENKNSIQNLLTP
                     IQTKMKEFQEKVEKFHLEDETGRASIKVEFEHLKEVNTNLSQDAQNLTTALLGDSKQQ
                     GDWGEMILEKCLQNAGLIEGEHYQKQTQIKTDDGTQLRPDVIIKLPNDRILIADSKVS
                     LKAYNSYMSADDEQKRKAYAKKHLQSVRNHIQGLSSKNYQEGFEEFGSPDYVLMFMQI
                     EPAYSLAQSLDNNLTSDAFIKNIIIVTPASLMMALRIVNQLWRQEKQVQNVKEIFERG
                     GKLYEKICGFLEEFEKIGDKIKGAHESYENAYNKLSDGRGNMIRQAEMLKNLGVKTAK
                     SIPREFKKAIEDSSQGNS"
     gene            complement(1650..7259)
                     /gene="uvrA"
                     /locus_tag="EYO68_02145"
     CDS             complement(1650..7259)
                     /gene="uvrA"
                     /locus_tag="EYO68_02145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIC06634.1"
                     /translation="MSQEKADQLVVIGASEHNLKNVSLNIPRDSLTVFTGVSGSGKSS
                     LAFDTIFKEGQRRFVESLSAYARQFLGQTDKPKVEHVEGLSPTISVDQKTVNRNPRST
                     VGTITEIYDHYRLLFARLGKPHCPECGTRITSQTPEQITDYAYVDALNEACLILAPMV
                     QERKGEYRKELEDWAAEGYVRARIDGEVRRLDEDIRLARYEKHSIELVVDRLTITAEE
                     KSRFTESVEKALLLGNGLAILHYGKKTAENETKNQLEQSPEKDHLFSQLMACPSCQIA
                     LPEMEPRLFSFNAPQGACPSCNGIGQLSTFSEEKLCDPELSISEGAIKCFTEKGNLLY
                     TKVDQRHVNSLMNNFEINNKTLWKNLSVEQRQLILYGNTKMKLGVSNIFRFPGQFLKK
                     LEKQQWAGFIPILQFVFRFVKGPLEKFQHTSICPDCEGKRLNKMALAVRLHGHNINSL
                     SGESIEDSVNFFDNLKLTETEKKIGRDIFREIRDRLHFLNDVGVGYLTLERSAATLSG
                     GEGQRIRLASQLGAGLQGVLYVLDEPSIGLHQSDNKKLIRTLKKLRDRGNTVLVVEHD
                     KETIESADHLVDIGPTAGQDGGHITAQGSMQKIIEAQESITGQFLSGQEQIAIPEKRR
                     QPTGNKITIHGASFNNLNNIDCEIPLGIFVAVAGVSGSGKSSLIDGILKKALNCHLNP
                     GSKDEPGPHKKISGLENIDRVIKIDQAPIGRTPRSNPATYTKVLDPIRDLFALMPEAK
                     ARGYKKGRFSFNVKGGRCEDCQGAGLKTIEMQFLSDVQIPCDTCRGKRFNAETLQIFY
                     KNRTIHDVLEMTVTEAEDFFSALPKIHIILQTLLDVGLGYVKLGQPSTTLSGGEAQRV
                     KISSELKKRATGNTLYILDEPTTGLHFKDIRLLLSCLNRLVDQGNTVLVIEHNLDVLK
                     VADHLIELGPGGGKYGGELVCEGTPENLSEQMTLTGKFLKTVLGKGAEQNSFKESEEK
                     SGKVSELQSFKVSATEVIEEDPADFEKVKVSKSYSRDIVIKGASKNNLRHIDVRIPNN
                     KMTVITGVSGSGKTSLAFDTIFAEGQARYLESLSTYARRFLGRMDKAPVDSIDGLSPA
                     IAINQKATGRNPRSTVATTTEIYDYLRLLFARIGKAHCPTTGKPLLGYSPTRAAAFCT
                     EHFEGERIELLAPLFLPGSSKILLLDHPKHLPNVIDSLEQEGFVRLYINGKAIRLDEW
                     KENSAEFKLSKNTTVDLGIDRLRVNPEEQKRLAEAIENAFQRGHGLLKICLTDLKPEG
                     QLSEKYVFLSETPANVENDVRLSFFQQEELSPRMFSFNSHVGACPTCDGLGEFQRIND
                     PQCEDCGGERLKAEYRAVTINQQNISQFCQLSVPEARQEIESWVLTENQRTVAEQALG
                     EILTRLKFLENVGLDYLTLDQKASTLSGGEAQRIRLASQIGSGLVGVTYVLDEPTIGL
                     HPRDTARLIRTLKQLRDRGNSVILVEHDLDTIRQADHIIDIGPGAGHYGGNVSAFGSP
                     QEISIKHDTLTAQYMRGEKSIPLPKKYRSFNPEHLISVTGAAANNLKKLDVNFPLGVF
                     TVVTGVSGSGKSSLVVDVLQRALEKEINGKQIKPGTHKKISGIAQLESIMVINQEAIG
                     RTPRSNPATYAKVLEPIRNLFASMPEAKQRGFSKRRFSFNAKEGRCPACDGQGFLLIE
                     MHFLSDVWVKCDQCKGKRYNRETLAVKYKGHTIADVLEMEISKAVEVFVAQPRIKRIL
                     KTLEDVGLGYMKLGQAGNTLSGGEAQRLKLAAELARRTHGPTLYILDEPTTGLHIDDV
                     ARLLKILHSLVDEGHSVLVIEHNLEVIKTADWLIDLGPEGGDKGGRLVFEGTPENCSK
                     NQLSYTGKFLEASFYSNPQSEFRETSL"
     gene            7512..10025
                     /locus_tag="EYO68_02150"
     CDS             7512..10025
                     /locus_tag="EYO68_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC06635.1"
                     /translation="MESIPKQAQVVIIGGGVIGCSVAYHLTKLGWKDVVLLERKELTC
                     GTTWHAAGLVGQLRATKNMTKLAQYTSELYATLEEETGQATGFKQNGSISVAPDVGRF
                     EELKRGASMAKCFGLEVEVISPREARELYPLLNIDDLVGAVFLPKDGQTNPIDVTQAL
                     AKGAKMGGAKIFENMRVCGIETKNAGTSGARQVSGVKVEVPSLGSGEASELKTISAEI
                     VVNCAGLWGHQVGKMAGVNVPLHAAEHFYIVTENIGLPSTLPVLRDPSGWIYAKEDAG
                     KMLVGCFEPKSKPRPLNTIPEDFSFGQFEEDWEHFEPAMLNAMHRIPKLEDAGIHTFF
                     NGPESFTPDDRYILGEAPELKNFYVAAGLNSIGIQSAGGAGKVLSEWIVNGYPPIDLW
                     DVDIRRFHSFQGNSRYLKERTEESLGLLYAMHWPFRQPETSRGVRKSVLHDRLEKAGA
                     CFGEMCGWERANWFAPEGTEPRYEYSFARQNWFDASASEHQAAREKVALFDMSSFAKF
                     VVQGRDAEKVLNRICANDVAVPSGKAVYTSWLNERGGIESDLTVTRLQENVFLVVTAG
                     ASQIRDLAWLHKNIPDDAHVTVTDVTSGYAVLSVMGPESRNLLSKLTPDDLSNEAFPF
                     GNAQEIEIGYARALALRMTYVGELGWEIYAPTEFAQDIYDKILAAGTEHGLKLAGMHA
                     LNSLRLEKAFRHWGHDIVDEDTPLEAGLGFAVKFDKPGGFIGREALLRQKDELSKTKS
                     GVLKKRLVQFALENPEPMLYHNEPIWCNNKIVGDLTSGMYGHTIGTCLGMGYVSHEDG
                     VSKEFLESSVFEIEVAGERYGARPSLRAFYDPKSKRVRM"
     gene            complement(10086..10817)
                     /locus_tag="EYO68_02155"
     CDS             complement(10086..10817)
                     /locus_tag="EYO68_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIC06636.1"
                     /translation="MTTDNNLQHFAEDMPLSKTALDLSSNGQGFGLIIVDEVNGFATV
                     GAGNLAPQVPNEQVSVMVSETNRLAHSFTQQAMPVLAFLDTHDSGKQEPPYPPHCESG
                     TGEEDLVPELKWLEKEPQATLVRKDCINGFIGAFQQDGSNAVIDWVKDNNVANVLVVG
                     ICTDICVMDFVLTLLSARNHGMLPSLKEVVVYDKGCSTYDLPRNVVEEIGLPPSASHP
                     QDVTHYMGLYFMASRGAQLVESVQV"
BASE COUNT         2827 a         2405 c         2392 g         3197 t
ORIGIN      
        1 ctcaggatta ataatttata gccctgtttc caaagtcgat catgagtttc cctgagatga
       61 atcttcaatg gcttttttaa actctctggg gatagacttg gccgttttca cacccagatt
      121 cttgagcatc tctgcctgtc taatcatgtt acctcttccg tcactcagct tattatatgc
      181 attttcataa gactcatggg cacccttaat tttatcacca attttctcaa attcttccag
      241 gaatccacaa attttttcat ataatttccc gcccctttca aaaatttctt tcacattctg
      301 cacctgcttt tcctgtcgcc atagttgatt tacaatccgg agcgccatca ttaaacttgc
      361 cggagtgaca atgatgatat tttttataaa cgcatcagaa gttaagttgt tgtcaaggga
      421 ctgcgccagg gaatatgccg gctctatctg catgaacatc agtacgtaat ctggtgaacc
      481 aaattcttcg aagccctcct ggtaattttt tgaagacaat ccctggatgt gatttcgcac
      541 ggactgcaga tgttttttgg cgtaggcttt ccgtttttgt tcatcatctg cagacatgta
      601 gctgttatat gctttcagtg agaccttgga atcggcaatc aggatacgat cgtttggcag
      661 cttgatgata acatccggac gcagttgagt gccgtcatcc gttttaatct gggtttgttt
      721 ctggtagtgt tctccttcaa tcaagcctgc attctgcaag catttttcca ggatcatttc
      781 accccagtca ccctgttgct ttgaatcacc tagaagtgcc gtcgttaggt tttgagcatc
      841 ctgactcaaa tttgtattaa cttccttgag gtgctcaaat tctaccttaa ttgaagcccg
      901 gccagtttca tcttcaagat gaaatttttc tactttttcc tgaaactcct tcatttttgt
      961 ttgaattgga gtaagcagat tctggatact atttttgttt tcctcaacaa attgttttga
     1021 tttgcttgta aaaattttgg cagaaagatt ttcaaactct gttcccatca atttttttgc
     1081 ttcctccaga aattttaatt tttctctgga ttcattttcc ttggattcca gcctttcttc
     1141 caaagttgca tttaatacct gcagggtaga aaactgtttt tgctgaaatt tcagttcttc
     1201 ctcaatttcc ggaattctgt ttgcatcctg ctttaagatg gcaaactgct tatcgagctc
     1261 tatcttgtct gccaggatgg tatcgtattt tttcagaagt tcatttagtg attcaccaag
     1321 tgcatctatt tcctgggatt gggtagaaat catttttttc tgatagaacc agcagaaaaa
     1381 aatccccagc aaaatacaaa atgcagtata tagaattaag gtgtaattaa tggaatcaat
     1441 gttcatggtt gtaatattta ttcaacagca tttagcaaaa agttattcta aacttgcctc
     1501 taatactata acaattgaaa gaagtcttga aaacaatttt gcccttgaaa gcaaattctg
     1561 aaaccaggga acgttatcgg cctggttcac tatgattctt caaagtttta ttaaaagttt
     1621 ctgatggaaa taagctttgt taaaaaagat cagaggctag tttccctaaa ttcgctttgt
     1681 ggattggagt aaaaagacgc ttccagaaac ttaccggtgt aacttagctg attcttactg
     1741 cagttttccg gagttccttc aaagaccaat ctgccgccct tgtctccgcc ttctggtccc
     1801 aaatcaatca gccagtctgc ggttttgatc acttctaaat tgtgttcaat gaccagcaca
     1861 gagtgacctt catccaccag gctgtgcaat attttaagca accttgccac gtcgtcgata
     1921 tgcaatccgg tagtgggctc gtccaaaata taaagagtag gaccatgtgt acgacgagca
     1981 agttctgcag ctaattttaa tcgttgggct tctcctccgg agagagtatt tcctgcctgt
     2041 cccaatttca tgtaacccag gccaacgtct tccagagttt tgagaatccg cttgatgcgc
     2101 ggctgtgcca caaaaacttc aactgctttg ctgatttcca tttcaagcac atccgcaata
     2161 gtatgcccct tatacttgac tgccagggtt tcccgattgt agcgcttgcc tttgcactga
     2221 tcgcatttga cccatacatc tgacaaaaaa tgcatttcaa tcaataggaa accctgtccg
     2281 tcgcatgctg gacagcgccc ctcttttgcg ttaaacgaaa aacgacgctt ggaaaatccg
     2341 cgttgttttg cttccggcat tgaagcaaag aggttgcgga tcggttcaag aacttttgca
     2401 taagttgcag gatttgaacg cggagttcgc ccaatcgctt cctggttgat caccattatg
     2461 gattcaagtt gagctattcc gctaattttt ttgtgtgttc ctggtttaat ctgctttcca
     2521 tttatttcct tttccagagc tcgctgcaga acatcgacaa ccagtgagga ttttcctgaa
     2581 ccggaaacgc cggtcaccac cgtaaaaact ccgagaggaa agttgacatc cagttttttt
     2641 aaattatttg ctgctgctcc ggtaacagaa attaaatgct ccggattaaa cgaacgatat
     2701 tttttcggca gcggaatcga tttttcccct ctcatatatt gtgcagtcag cgtatcgtgt
     2761 ttgattgaga tttcctgtgg acttccaaaa gcggaaacat tgccgccgta atgacctgct
     2821 cccggaccaa tatcgataat atggtcggct tgtcggatcg tgtccaaatc gtgttcgact
     2881 aagatcacac tgtttccacg atcccgtaat tgcttcagcg tgcggataag ccgtgcagta
     2941 tcacggggat gcaggccaat agttggttca tccaaaacat aggtcacgcc caccagtcct
     3001 gaaccaattt gtgaggcaag ccggatacgc tgcgcctcac ctccggaaag tgtggatgct
     3061 ttttgatcga gtgtcaaata gtccaaccct acattttcca aaaatttaag acgtgttaaa
     3121 atttcgccca gcgcctgttc ggcaacggta cgctgatttt cggtgagaac ccagctttca
     3181 atttcctgac gtgcttccgg gacagaaagt tgacaaaact ggctgatgtt ttgctgatta
     3241 attgtcactg cacgatattc tgctttcaaa cgttcacctc cgcaatcttc acattgagga
     3301 tcatttatgc gctgaaactc tcccaaacca tcacaggttg gacatgctcc cacatgagag
     3361 ttgaaggaaa acatgcgtgg tgataattct tcctgctgaa aaaaagaaag cctcacatca
     3421 ttttccacat ttgctggagt ttcagacaaa aatacatatt tttcactcaa ctgaccttcc
     3481 ggttttagat ccgtcaaaca aattttcaac aaaccatgtc cacgctggaa agcattttca
     3541 atggcttctg ccaggcgttt ttgttcttcc ggattaacac gcaaacggtc aattcccaaa
     3601 tcgacagtag tgtttttact gagtttaaat tctgcggagt tttccttcca ttcatccaga
     3661 cggattgctt ttccattgat ataaagccgc acaaatcctt cctgctccag cgaatcgatc
     3721 acatttggca agtgcttggg atgatccagc aacagaattt tgctcgaccc cggtaaaaat
     3781 aaaggtgcaa gcaactcaat ccgttcacct tcaaaatgtt ctgtacaaaa ggctgccgcg
     3841 cgggttggac tgtagccgag caaaggtttt ccggttgttg gacaatgtgc cttgccgata
     3901 cgcgcgaaaa gtaaacgcag ataatcatag atttcagttg ttgtagcaac agtcgaacgg
     3961 ggattacgac cagttgcttt ttgattgatg gcaattgccg gagaaagtcc gtcaatcgaa
     4021 tccaccggtg ctttatccat acggcccaga aatcgacgtg cataagttga aagcgattcg
     4081 agataacgcg cctgtccttc cgcaaaaata gtgtcaaagg ccaaactcgt tttgccggaa
     4141 ccggaaacac cggttatcac agtcatttta ttgtttggaa tccgtacatc tatgtgccgc
     4201 aaattatttt ttgacgcacc cttgattacg atgtcacgag agtatgattt tgaaactttt
     4261 actttttcaa aatccgccgg atcttcttca atcacttcag tcgctgaaac tttgaaactt
     4321 tgcagctctg aaactttgcc acttttttct tctgattctt tgaaactatt ttgttcagcc
     4381 ccttttccca aaactgtttt caaaaacttt cctgtcagtg tcatctgctc cgataaattt
     4441 tccggagttc cttcgcaaac cagttcacct ccatatttcc cgcctcccgg tccaagttct
     4501 atcagatgat cggcaacctt cagcacatcc aaattgtgct caatcactaa aacagtgttg
     4561 ccttgatcaa ccagacggtt caggcaagat aacaacaaac ggatgtcctt aaaatgcagg
     4621 ccggtggttg gttcgtcaag aatgtaaagc gtatttccag tcgctctttt tttcaactct
     4681 gaagaaattt ttacacgctg tgcttctcct ccggagaggg ttgtggaggg ttgcccaagt
     4741 tttacatagc ccagaccaac gtccagtaga gtttgcagaa ttatgtggat ttttggtagt
     4801 gcagaaaaga aatcctccgc ctctgtaacc gtcatttcca gtacatcgtg aattgtgcgg
     4861 tttttataga atatctgcag agtttcagca ttgaagcgtt ttccgcggca ggtatcacaa
     4921 ggaatttgta catcactcaa aaactgcatt tctatcgttt tcaaaccggc accctgacag
     4981 tcttcgcagc gtccgccctt gacgttgaat gagaagcgtc cttttttata accccgggct
     5041 tttgcttcag gcatcagggc aaataaatca cggattgggt caaggacctt ggtgtaagtt
     5101 gctgggttgc tgcggggggt gcggccaatt ggagcctgat caattttaat cacacgatct
     5161 atattttcga gtccggaaat ttttttgtgc ggaccgggtt cgtctttgct tccggggttc
     5221 aggtggcagt tcaaggcctt tttgagaatg ccgtcaatca acgaggattt acctgaaccg
     5281 gaaacacctg ctacagcaac aaaaattcca agtggaattt cgcagtcaat attgtttaga
     5341 ttattgaaac ttgcaccgtg aatagttatc ttgtttcctg ttggctgacg acgtttttcc
     5401 ggaatggcta tttgttcttg accggaaaga aattgacctg ttatcgattc ctgtgcttca
     5461 attatttttt gcatggaacc ttgtgctgtg atgtgtccac cgtcttgtcc tgcagtcgga
     5521 ccgatgtcca ctaaatgatc ggcagattcg atggtttcct tatcatgctc aaccaccagc
     5581 acagtgtttc cccggtcacg taattttttt aaagtgcgaa tcagcttttt gttgtcactc
     5641 tggtgcagtc caattgaagg ttcatcgagt acataaagca caccctgcag tcctgcacca
     5701 agctgtgaag caagccggat acgttgtcct tctccgccgg acaaggttgc tgcagaacgc
     5761 tcaagtgtga gataacctac gccgacatcg ttcaggaaat gcagacggtc ccggatttct
     5821 ctgaaaatgt cccgccctat ttttttctca gtttcagtca gttttaaatt atcaaaaaag
     5881 ttgaccgaat cttcaattga ttcaccggag agactattga tattgtggcc gtgcagtcgt
     5941 actgcaagtg ccatcttgtt gaggcgcttt ccttcacagt ccggacaaat agaagtgtgc
     6001 tgaaattttt caagtggacc cttgacaaaa cgaaaaacaa attgcaggat tggaatgaaa
     6061 ccggcccatt gttgtttctc cagttttttg agaaactgac cgggaaaacg aaaaatattg
     6121 gaaacgccaa gcttcatttt agtgtttcca tataaaatca actgccgctg ttcaacggat
     6181 aaatttttcc acaaagtttt gttgttgatt tcaaaattat tcattaaact gttgacgtgg
     6241 cgctgatcga cttttgtgta tagtaaattc cccttttcag taaaacattt aattgcacct
     6301 tcagaaattg agagttcggg atcacagagt ttctcttcag aaaatgtact taactggccg
     6361 attccgttgc acgagggaca tgctccttga ggtgcgttaa aagaaaataa gcgtggttcc
     6421 atttccggca gcgcaatttg acagctggga caggccatca actgactgaa caagtgatct
     6481 ttttccgggc tttgctccag ctgatttttt gtttcatttt ctgcagtttt tttaccatag
     6541 tgcaaaatag ccaacccgtt acccagcaac agggcttttt ctacactttc tgtgaaccga
     6601 cttttttctt ctgcagtaat tgtaagtcga tcgacaacca gttcaatgga atgtttttcg
     6661 tagcgggcaa gccggatatc ctcatccagc ctgcggactt ctccgtcaat tctggctcga
     6721 acgtagccct cggcggccca gtcttctagt tctttgcggt attctccttt gcgttcctga
     6781 accattggtg ccagtatcag gcaggcttca ttcaaggcgt ccacataagc ataatcggtg
     6841 atttgttccg gagtttgcga ggtgatgcgg gttccgcatt caggacaatg cggcttgccc
     6901 aaacgtgcaa aaaggaggcg ataatgatcg taaatttctg tgattgttcc aacggttgaa
     6961 cgcggattac ggttgactgt tttctggtct acggatatcg tcggactcag tccttcgacg
     7021 tgttcgactt taggcttgtc agtttgcccc agaaattgac gggcatacgc cgaaagtgat
     7081 tcaacaaaac ggcgctggcc ttctttgaaa atcgtatcaa atgccagtga agattttccg
     7141 gaaccactga ctccagtaaa aactgtcagt gagtctcgtg gaatattaag gcttacattt
     7201 ttcagattgt gttctgatgc gccaattacg actaattgat ctgctttttc ttgggacatg
     7261 aaaaaggact gaggatattc tggacaggaa attaagctga tttttgatgt ttattttgtg
     7321 atcattatgg caggaattat ttggagcaac aagacggaat ttattttgaa atcaaactga
     7381 attagtatgg tgaaaaaact tatttggtta caccaaattt gtggttgacc atgcatgcag
     7441 gaagctgtaa aacttattca tctttatttg tatttgttac attgtttaaa aatattatta
     7501 ggaatctcta aatggaatct ataccaaagc aggcacaagt tgtcatcatc ggcggcggtg
     7561 tgatcggctg cagtgtggct tatcatttaa caaaacttgg ctggaaggat gtcgttttgc
     7621 tggagcgcaa ggagttgacc tgcggtacaa cctggcatgc cgccgggctc gtgggtcaat
     7681 tgcgggccac gaaaaacatg accaaacttg cgcaatatac cagcgaactt tatgccactc
     7741 tggaagaaga aacaggacag gccaccggtt ttaaacaaaa cggttcaatc agcgtggctc
     7801 cggatgtcgg gcgttttgaa gaactaaaaa ggggagcctc gatggccaaa tgttttggtc
     7861 tggaagtcga ggtgatttct cctagagaag cacgtgaatt gtatccgctt ttaaatattg
     7921 atgacctggt cggcgctgtt tttttgccca aagacggaca gacaaatcca attgatgtga
     7981 ctcaggcgct ggcaaaaggc gcaaaaatgg gtggagcaaa aatatttgaa aacatgcgcg
     8041 tttgcggaat cgaaaccaaa aacgccggaa cgtccggtgc gagacaggtt tccggagtca
     8101 aagtggaggt tccatctctc ggtagtggag aagcaagtga actcaaaact atctctgccg
     8161 aaattgttgt taactgtgcc ggactatggg ggcatcaggt tgggaaaatg gcaggcgtaa
     8221 atgtaccgct gcatgctgca gaacattttt atattgtcac agaaaacatt ggtctgccgt
     8281 caactcttcc agttttgcgt gatccaagcg gttggattta tgcaaaagaa gatgctggaa
     8341 aaatgctggt tggctgcttt gaacctaaat caaaaccgcg cccactaaac acgattccgg
     8401 aggatttttc atttggtcag tttgaggaag attgggagca ttttgaaccg gcgatgctga
     8461 atgcaatgca tcgcattcca aaactggaag atgcaggaat ccacactttt ttcaacggtc
     8521 cagaaagttt cactccagac gaccgctata tcttaggtga ggctccggaa cttaaaaact
     8581 tctatgttgc cgccggactc aactcaatcg gaattcaatc tgcgggtggt gcaggcaaag
     8641 tgctttccga atggatcgta aacggatatc cgccaataga tttatgggat gtagacatcc
     8701 gccgttttca ttcgtttcag ggcaattcac gatatttgaa ggagcgcacc gaagagtcat
     8761 taggcctgct ttacgccatg cactggcctt tccgacagcc ggaaaccagc cggggagtgc
     8821 gcaaatccgt tttgcatgat cgtctggaaa aagcaggagc gtgttttggc gaaatgtgcg
     8881 gatgggaacg cgccaactgg tttgctccgg aaggaaccga accgcgatac gaatacagtt
     8941 ttgcacgtca aaactggttc gatgcttctg cctccgagca tcaggcggca cgggaaaaag
     9001 tggcgctgtt cgacatgtcg tccttcgcga aattcgtggt gcaagggcgt gatgctgaaa
     9061 aagtgctcaa ccggatttgt gccaatgatg tcgctgttcc atccggaaaa gcggtttata
     9121 catcgtggct caatgaacga ggcggtatcg agtccgactt gacggtaacc cgtttgcagg
     9181 aaaatgtttt tctggttgtt acggctgggg ctagtcaaat ccgtgatctg gcctggctgc
     9241 acaaaaatat tccggatgac gcacatgtga cagtcacgga tgtaacttcc ggatatgctg
     9301 tgttgagcgt gatgggaccg gaatcccgaa atttactttc aaaactcact ccggacgatt
     9361 tatcaaatga ggcctttcct ttcggcaatg cacaggaaat tgaaatcggc tacgccagag
     9421 cactggcctt acgtatgact tacgttggtg aattgggctg ggaaatttat gcaccaaccg
     9481 aatttgctca ggacatctac gataaaatcc ttgcagctgg aaccgaacat ggtttgaaac
     9541 tggcgggaat gcatgccctc aactccctgc gtttggaaaa agcatttcgg cactggggac
     9601 acgatattgt ggacgaagac acgccactgg aagccggact tggttttgcg gtcaagtttg
     9661 acaaacccgg aggtttcata ggtcgggagg ccttgctgcg tcaaaaagac gaattgtcca
     9721 aaacaaaaag cggagttttg aaaaaacgtc tggtgcagtt tgctctggaa aatccggaac
     9781 caatgcttta tcacaatgaa ccgatttggt gtaataacaa aatcgttggt gatctgactt
     9841 ccggaatgta cggtcacacc attggaactt gtcttggaat gggttatgta tcacacgagg
     9901 atggagtgag caaagaattt ctggaatcaa gtgtttttga aattgaagtg gccggagaac
     9961 ggtacggtgc aagaccttct ttgcgtgcgt tttatgatcc gaagagcaaa cgggtacgga
    10021 tgtagagagt caatttttca aaaaatattc aagttgcagc taaattgctt gagctgcaga
    10081 aaatttcata cttggacact ttcaaccagt tgcgcacctc gtgaagccat aaagtagagt
    10141 cccatgtaat gagttacgtc ttgagggtgg ctggcactgg gtggtagtcc aatttcctca
    10201 acaacatttc tgggcaggtc ataggtagag cagcctttgt cataaaccac cacttctttc
    10261 aaacttggaa gcatcccgtg attgcgggct gagagcagtg taagaacaaa atccattaca
    10321 caaatgtcgg tacatattcc aactaccagg acattagcca cattattatc cttgacccag
    10381 tcaataactg cattggatcc atcctgctga aacgcaccaa tgaaaccgtt aatgcagtcc
    10441 ttgcgtacta aagttgcctg tggttctttt tcaagccact tcagttccgg aaccaaatcc
    10501 tcctcacctg taccgctttc gcaatgcggt ggataaggcg gttcctgttt accagaatcg
    10561 tgtgtatcta gaaatgccag cacaggcata gcttgttgag tgaaagaatg cgcaagacga
    10621 ttagtttcgg aaaccatcac tgatacctgt tcattgggaa cttgaggagc aagatttccg
    10681 gcgccgacgg tggcaaaacc gttgacctcg tcgacaatga tcaaaccgaa tccctgtccg
    10741 tttgagctca aatcaagtgc agttttactc aggggcatgt cttcagcaaa atgctggaga
    10801 ttgttatctg ttgtcatgtt t
//
LOCUS       DTVD01000059           33203 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_152_length_33203_cov_369.713648, whole genome
            shotgun sequence.
ACCESSION   DTVD01000059 DTVD01000000
VERSION     DTVD01000059.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 33203)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33203)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33203
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_33203_cov_369.713648"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(142..3204)
                     /locus_tag="EYO68_02160"
     CDS             complement(142..3204)
                     /locus_tag="EYO68_02160"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03710.13,HMM:PF08335.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06637.1"
                     /translation="MLTHKTKNKFNVTLNDWVTNLFPLSKDKDWKAILQESSAPFTSE
                     SAKRLVDLLEGSLAIFENETQLAQYLPILLSSGTPEAALTQLHDFADAFRNKFSSNFD
                     WSRPDTGALLYIFGRSNFLANRLKRNPQLAVGLLESPFLLRKKDLKVMENELRTRLNL
                     QTEYSLLEFKNILRRYKYEEFLRITVRDLAQLCPFKETLEELSAIAICCLRVAISGIT
                     NHELEISNYSISSSIPDSSVATNRESSQSSKESPQQLFPFMILGMGKLGGNELNYSSD
                     LDLIFIHDNEPLTGDTERDYKLRMKAARILIEVMSEVTDEGFLARMDMRLRPGGERAP
                     LVQSLDEMEYYYTVSGELWERQSLIKAVPVAGTKQLGKDVMNMITPFVFRSLLDEGVL
                     RDVEKVKKRIEEEHLRESFLNVKLGIGGIREIEFFVQTFQLLYGGGNKQLRLAGTLQV
                     LQQLRQSELIPKQDADTLEKAYFFLRRVEHHLQLREEQQTHTLASDIVQQHAIARNLG
                     YNEFDIEKAHQHFLSDLKDVMGGVRAIFSGLFSSKHLEIEAAIHNCARIQNFTEEEQR
                     FIESFSQQLAPLMSESTKNRFQRLFESIKGKIGCYRKLSVHPSALSRLTRIAETSEML
                     WNYLLNHLELLEQLDNYTMEISVEKWTDQLEENIQSCAENEEEEIDQLRQFKHTTTFL
                     LGSAEMEGVLSYERTRIALTLLAEVIVQAAFRLSQKWLTQRYGKVLNSMDESGQFAII
                     GLGKLGGRELTYHSDLDLIFIYSGEGTTSGPRVIGAQEYWVKLIQRLISCLSTITSSG
                     YAYKLDARLRPSGKAGVLVTHLNQYLKYHETSQPWEHQVLIKGRVIGGSGEVEWYQKV
                     EEGIRSAVYDWIPPSDMNAQINYLRRRKELELSGEKDNRRNIKEGKGGLLDIEYLTQA
                     LQLKFGSSFPQLQNPKTMEVLPELGKLALLKKEEAKSLQYNYKMLRLIENGLRLIYDE
                     STDLLDFEKVQEDTILKLLKHHGYEVSNLRDTVETSTQNVREIYLKYF"
     gene            complement(3478..4887)
                     /gene="glnA"
                     /locus_tag="EYO68_02165"
     CDS             complement(3478..4887)
                     /gene="glnA"
                     /locus_tag="EYO68_02165"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIC06638.1"
                     /translation="MTTPKEIVEFAKQNDVEMVDLKFIDFLGTWQHFTVPVSELNEEM
                     LDEGRMFDGSSIRCWQTIDNSDMKVVPDLDTVKMDPFYEVATMSIICDIKDPVTGEGY
                     TRDPRSVTKKAENYLISTGIGDTIFLGPEAEFFMFDDVRYAYGPEGGFYSIDSPECPW
                     NTGVEEVGGNKAYKIGHKSGYFPAAPFDQDRDIRAEMVLAMQELGLTIEAQHHEVAPA
                     QHEIDFKFDTMLKTADMLQHFKYVVRNVAQEYGKTATFMPKPMFADNGSGMHTHQSIW
                     KDGQPLFAGDQYAGLSEMALYYIGGILKHAPALAAFTNPLTNSYKRLVPGFEAPINLA
                     YSNRNRSATIRIPVADSPKGRRIEYRCPDSGANAYFAFPAIMMAGLDGVQNKIDPGEP
                     MDVNIYDLPPEKLNKIGKMPSSLNEALDALKNDHEFLLKGEVFTKDVIEYWLDWKMEE
                     VRAIDSRPHPHEFELYYHY"
     gene            5228..5998
                     /gene="flgF"
                     /locus_tag="EYO68_02170"
     CDS             5228..5998
                     /gene="flgF"
                     /locus_tag="EYO68_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HIC06639.1"
                     /translation="MSGITSLVQAAGVIEHKMETVSNNLANVNTVGFKEDQPSFREVL
                     SNVQRIAPESLEERFLSHEYLDDYVGMDKSAVVVDEVGKNFEPGRMRSTGNNLDFAIS
                     NEGFFTISTPQGERFTRAGNFQLDSTGRIVTNDGYPVLGENGVITIKEGSIDVSESGL
                     LSVDGIIADSFRLVRFRNQDQLQKLGQGFFAPVNSNDLPVASDGIKLRQGMLEDSNVN
                     SMLEMTRMITATRAYESVHKALSRIDRLDEKAISLVQS"
     gene            6061..6849
                     /gene="flgG"
                     /locus_tag="EYO68_02175"
     CDS             6061..6849
                     /gene="flgG"
                     /locus_tag="EYO68_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HIC06640.1"
                     /translation="MMRALYTAATGMQAQQRNIDTTANNLANVNTTGFKKSKVQFQDL
                     LYYNERSAGVPSSVSTQVPVGIHVGHGVKYVSIDKVHTQGNMENTGNDLDLSIDGMGF
                     FQILQPNGIIAYSRDGHFNIDGMGRLVTNDGMLMDPEINIPADTRKVQIGLDGTVSIF
                     LRDENLPEALGSIQLARFQNPTGLTPIGKNLFLSTPASGNAIVDAPGSSNMGSLTQGF
                     LERSNVSLVEEMVNMITAQRAYEVNSKAIQSADEMLKNTNNLIR"
     gene            6936..7970
                     /gene="flgA"
                     /locus_tag="EYO68_02180"
     CDS             6936..7970
                     /gene="flgA"
                     /locus_tag="EYO68_02180"
                     /inference="COORDINATES: protein motif:HMM:PF13144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HIC06641.1"
                     /translation="MPSLAHLIIKTKGIAMRPYPYRYGLLLLLFLLYSGTVFSKGNTA
                     PEAQQIKIFLPQSSVINSDEYRLGEIAQLEGEDFILLDRLAKVVIGRAPLPGRKLTVT
                     RSLILSRLRSHKVNIKKFVFPGSDSTSIQRAALKISGNDIEQVVLNHIKETKGFSDLK
                     PRLLAKIRDIFLPRGQVSYVISSKGNYKKEGGYRNYEVEFSVDGKAIRKVSVRTYLKI
                     YKEVYVARNTIKRNHIIEESDLLKIRKNVDRMPREYVTDKEQLIGKISTRTITPSEAI
                     QGNTLSDPPLVKSGDRLQIVFETPFIRLSAPGISLAKGRKGERIPVKNADSKIVVFAT
                     VKTRNIVLVN"
     gene            8013..8783
                     /locus_tag="EYO68_02185"
     CDS             8013..8783
                     /locus_tag="EYO68_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgH"
                     /protein_id="HIC06642.1"
                     /translation="MYKLLIPFSGLFHWFLATFLVLSLIGCVPSRPQPKPPAEVLAAY
                     EVKKMNDNLKRTAKRLRRKRISTSRNRYEGSLWRYESSFGNLLRDHRARFRGDLLSIN
                     EMSLIVNVPPPKTPAEGQPAGGAETANVALEALTLRDSIEEEQNQILRNLDGISARVI
                     RVLPDGNMLIKGQKIDYRQRNQVRYITTVTGILRPADVNDSNVVSASKLANPNVKIKR
                     QQSGSLVRERLQKLAPLLGKQKAGFLGRLGDMAKGGAQ"
     gene            9289..12984
                     /locus_tag="EYO68_02190"
     CDS             9289..12984
                     /locus_tag="EYO68_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06643.1"
                     /translation="MRGSYHDVHLKKWKEYAQREFDEMGRINRLRLESLMQLSDQEMH
                     KTIFEGILLFDINPENVAPLELQEKTGEFDEEGNPVMSTMTFDVFQKGAIHGIEGLER
                     FLPSASIKGEAGMDAHLEEEFSGTDLMSNFKQESGQLIKSLTTIGSLGGIGHKPDSDM
                     DAQIIINSNPEFKFSWNDADFLVALIANVMESFYDDYYINGLTTQERLVIKKAAAGTL
                     REQCSTGLSEEEQQVIEFIFASSYRKELRKLIQEHIQKRPAEEQKQFFQKSVISTLNK
                     YPDCENFLEPLKKFFSFLKIGGGDLQQKAFPYSLKQLSKEKVLNCLTNYYRTTFLDEA
                     GARQILWRYGVNNNLAPESLPEEKKNECFLNSLTNNSQLSTLLTEFFEYLSSHVAYAS
                     MNKLSEAMQTLKQHFSSHNVVFKDGLEQQVLSKLEINYSSRTVRMIETFSNGQAKDLE
                     AEIEYPLHLKIQQAEAYLTKKYPTTKIHFFTNILRKQRAGQHTPFLVSPEGSMAYALM
                     LNDFLLNPAAMICGITPMPFDLPKNFKILSSIGVFPEAEWTLKQNLAAEYRKNNKVTE
                     NDTGEVQINKNVTEKNQILEEEKESFILGKLPNWGEIIIPREMFLGHAIPIFLRESEK
                     ISRRNLPKALLNCWWLEMIVCIDDEDELPTSLTRLLWNPEGRYFIRENRKGPLIDAIV
                     RMEDDYPALQLDPWWLKFTEMLVRFESYEQEEEEEPDFELNTLSETQKNIVFCFAQHM
                     RISDVINFGDDGNPVWLDENSTWRSRALVDFYKIFFSIPEDRRELIRFSEGRDDAGNK
                     MEKMLKKLFLESMTRVENKLCKIGHTRALTQISNQLARLSEKGFEKEKAANILSPLLD
                     VVNQRVSIEDRKVLVKLKKKIPLNKIEQMQAKIVYEELQKLKSVQGNIVDYFKQYDLI
                     MKESWVRKTITNAKVSVAGDPLENVIFKFHFERNFERKPFQVLLPISKSLSIPRSRIK
                     VEFVRKSEKWQFSSMLSRKEAGGGKSGAETVIPMFEENLVEGIARCTFSGYVGFGGKY
                     LSTFEKPAAQVHSDVAMNPVSGGALFTLATEIISFFSHFSVSSRELMENIHYIRDVLM
                     VCNVNKLNIISLIVRDNLGEQFVIAFDIRQIVIKKVPPKLRIGGDSALAEFFMRLNSR
                     ECRILFMHHLSALNIPIRASHLPRLRIWVNGANYKLPITPKFQQNYLNGIANTLWPND
                     SIGTREHLLPPPLTRTFDQIGRASLQG"
     gene            13113..14348
                     /locus_tag="EYO68_02195"
     CDS             13113..14348
                     /locus_tag="EYO68_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06644.1"
                     /translation="MASKEPIENPNTPNVNFIGFQKQLREIIKDIKQSNKKTGLKKIF
                     GGKKQVNPHVDGRILENDAPNNKEVSKLIKQLDSDPTDPLVRLRLVNAMMADRKVHHL
                     QTHLNMMLQASIPIYLSDITTTFLQLVMHTYRAYLERLANIHKHNMMAIRSEVLKNVN
                     MSGIDVEDEDTEDSHVKDTESMKTEIQIAEALIDNCETIIQRVKTKMTSTLSHEEIEE
                     LTSKEKASSSFFSGESEKVNPNKQNMIISKAVQAIEMLKQIPLLHGAGLDLAQQLGRI
                     DNKLTYPLVMEGRIQMQALKYQFLRIESGDRSARENMAPVYNLAVVAYRKALKLTSKT
                     TPKKSDLPVLTEFGNLTHYGFIHRDLMRFTEDGVMNLVKLGKDSVDAAVTVDESFVPL
                     QKRLESSLAQLNKVEGEHT"
     gene            14373..15401
                     /gene="tilS"
                     /locus_tag="EYO68_02200"
     CDS             14373..15401
                     /gene="tilS"
                     /locus_tag="EYO68_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIC06645.1"
                     /translation="MKLHVESLLQKIQKNWETYQLAVPENKQKNRNSQKGPLEVIVCV
                     SGGSDSVALFNLLQRLSTLLRLKLHVLHFNHQLRPEAQQEQDFVESLAKLHKIPFHYK
                     TAKHLKHGQPGLQESARNWRIEESCLLLESLGGGCIATGHHADDQTETFLLKWLRGSH
                     ISNLQGMRWKNTPFIRPLLNCSKLELQVFLKNNNLTWMEDSSNQSNAYLRNRVRLELV
                     PLLEELSRQGLQSRISDMSGQSKLLREWLDSQYEKWRLHCQNNKSEAPEVLSLTDLEK
                     ANDLLQQEIMHNFINSQTELKLSYAKLQNIFELIHTDNNNWQFRLSKKWSIFRSVNNL
                     ELHLNSES"
     gene            15493..16383
                     /locus_tag="EYO68_02205"
     CDS             15493..16383
                     /locus_tag="EYO68_02205"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HIC06646.1"
                     /translation="MRIGIDMGGTKIEGIALADTGEELIRKRIDTPRHDYDETVNAIA
                     GIVLNLESETKQKGTVGVGIPGAISPQTGLVKNANSTWLIGKHFDLDLESALKREVRL
                     ANDANCLAVSEAVDGAGAGSKVVFAIILGTGCGGGIAIDQEVHTGHQAIAGEWGHISL
                     GWMRPEEYPGTDCYCGQRGCLETFISGTGFENEYELQTGINKNGREIVEMLEHGDAAA
                     EKVMQIYENRLARAIATAVNCLDPDVLVLGGGISNISRIYKTVPQLVQQWTFGGEFST
                     PILPAKHGDSSGVRGAAWLW"
     gene            complement(16384..17127)
                     /locus_tag="EYO68_02210"
     CDS             complement(16384..17127)
                     /locus_tag="EYO68_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06647.1"
                     /translation="MQKKVKLQKTNAITVFGAGHQALAEIEGLRGMAVRGMFFNYLIF
                     FAVSLALNGLFYFQLLGPFINWLFGGDGFWAAVGTIVLWSIQLTVAAVIAMVSLRFSV
                     ELLSLWHQSLVQRIITHFRQIEEPVFSFKAWLAEINHILKEALKACLFPVLLIFVGLI
                     PVIGLPIVFMLESHLMGREGVIVYLDSLTNQEEAVELRKMWRWMPIRIGWLPAFLAFI
                     PFIGWLFLPLSLTYEVVGFAYLVEKSRNN"
     gene            17367..17903
                     /locus_tag="EYO68_02215"
     CDS             17367..17903
                     /locus_tag="EYO68_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06648.1"
                     /translation="MLTAFSAEAAELWRLRYFVPTSAESEITFGSSKAAEKLNTSGHS
                     GNLVFANGIGVGYSTLRSNVSLEGVSYIFRNDSLDLSYTIGNSLSFTFGAGRLINGRG
                     ELSLNGVSYATENSTGGAVFFNLGIPFIGGELLLGYRQNNIEYKNYQSQISGKTVTLA
                     DPVKLLSSQVSVGIGFIF"
     gene            18034..18456
                     /locus_tag="EYO68_02220"
     CDS             18034..18456
                     /locus_tag="EYO68_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
                     /protein_id="HIC06649.1"
                     /translation="MKRALELGEQVRNKTGDNPWVGCVIVRGEKVLGEGHTHPPGEAH
                     AEADAILNAEKQGHSLTGSTLYCTLEPCSFHGRTPSCAETIVEKQISHVVIAIRDPHP
                     KVNAEGVRMLRESGVLVTEGIEEQDARKSLKDWLLKYE"
     gene            18453..18893
                     /locus_tag="EYO68_02225"
     CDS             18453..18893
                     /locus_tag="EYO68_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIC06650.1"
                     /translation="MRKNIEYPERPIAGVGVVVFRNEEVLLVKRKKAPYKGQWSIPGG
                     KQRLGETVTQAARRELMEETGVEVNELTLIDVIDIIVPDEEGKILYHYIVADYRAHWL
                     SGECSPGDDAQDVQWFNLNKLGSISLLDKTRKIILKAAGKPDST"
     gene            complement(18944..19864)
                     /locus_tag="EYO68_02230"
     CDS             complement(18944..19864)
                     /locus_tag="EYO68_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIC06651.1"
                     /translation="MNYLKEDFQEFTNALRTLKFWYMLLTLTLGCFIFSFVVNGILLP
                     HNFFAGGLIGLALLFYDSIKEYISFSLLYALLNIPIFIIGFREFSLKFIITSLIGMGI
                     YSLSLQLTHGVEISINDPMLAAIFGGVLAGFGTGFYLRLGGSVGGLDIVGTVIKKRFA
                     IPIGTVFNLVNIVVIFSNAYLYHLETALFTGVFMYVFSWSMQKGQVGLSQRKSIFIIS
                     SKPDEVAEKVLKKLDRGFTYFHASGGFAGEEKQVIYTVINMLELGRLKEYLFDTDPEA
                     FISVQDTGDVIGKRFLTWEDEGFRKRKKDN"
     gene            complement(19969..20163)
                     /locus_tag="EYO68_02235"
     CDS             complement(19969..20163)
                     /locus_tag="EYO68_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIC06652.1"
                     /translation="MATGKVKWFNERKGYGFIEQESGKDLFVHHSEVRGGYLREGENV
                     EFEIGEGQKGPCAVSVQTAS"
     gene            complement(20286..22103)
                     /locus_tag="EYO68_02240"
     CDS             complement(20286..22103)
                     /locus_tag="EYO68_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06653.1"
                     /translation="MNKEVNDWELFQRFLVYVRPYWKSVVLGVATVPFSIAATLLLPW
                     LLIQIIDEHVIPGDLDGLFRMVVLMAGAVAVGYFSDSIYTFYLQKTGQLAISAMRSDL
                     YAHILSMPRSFFDQRPIGVILTRLTSDMEALNDSLAIGVLSIFTDFLKTIALLILLII
                     LSWKLTLVVLLILPPIYFVSNFLRSRLRHYYNLTREALADSTGYLQECLNGVKTIQLY
                     AAEAKVRKQFEAKTRHFFKAQTHSNFFDAALFSVIEGITSIALALMLWYGAQQILTGL
                     VSIGVLVGFINTLNRIFVPIREFTQQISVFQRSLSALENIDKLFREIPEDQDTADMPS
                     YATNSRKYTPEEIMKRFQEFEELRFENVHFRYTEDGPWVLQGVSFIVRKGDRLAIVGA
                     TGSGKSTIVRILTRTYTRYQGSIKINGVELYEISRDHLQLMIAMMQQDSHLFEESIGF
                     NIALNRPEVSPEKVKEAAKYVYAHTFIELLPEKYDFKILEGGKNLSEGEGRLIVFARA
                     LAGESDLIVLDEATSSVDSVTENLIQKAIERIFHDKTVIAIAHRLSTIRNSDLILVID
                     AGKIVERGSHAELMQLSGVYASLLETLEKETELVSVPAK"
     gene            complement(22225..23496)
                     /locus_tag="EYO68_02245"
     CDS             complement(22225..23496)
                     /locus_tag="EYO68_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC06654.1"
                     /translation="MNLENATKEELQTLEQKLAEKYQKYKDAALNLDLTRGKPGTAQL
                     ELADEMEGLLKDKLILEDGTDLRNYGGLDGIPAARKLGGEMLDLPETEVICGDHSSLS
                     LMYLYMLHAFYHGSQGPNTAWAREGEVKYLAVVPGYDRHFSICEELGLRMISVDMLDD
                     GPDMDAVEDLVRNDHMIKGMWCVPKYSNPTGNVYSEEVVERIAALGKISGSNFRVMWD
                     NAYGVHDLFENPQQLANVMDFCRKYGTVDNLILTASTSKITYAGGGISFLGASEKNLE
                     HFRKRLAVMSIGPNKLNQQRQVLFLKNLAGVLEHMRKHAEILRPKFAMVQKHLESELA
                     GKGVGTWSNPKGGYFVSFDALPGLAQEIIRLAGEAGVKLTPAGATYPYSHDPNDKTIR
                     LAPTFPSVEDLDQAMQIFVICVQLASIRQRL"
     gene            complement(23612..24463)
                     /locus_tag="EYO68_02250"
     CDS             complement(23612..24463)
                     /locus_tag="EYO68_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC06655.1"
                     /translation="MLYHTFSTQEEIDQEYNPRFIVENTDELIQSYFTESQRVLREYS
                     NRSAVAYGPTPSETLDIFPAEKLCSPIHIFFHGGYWHSLTSRDFAFVAEGLVRNGITA
                     VLVNYALCPSVNIDEIVRQSRAAAAWTYRNAEDFGGNPERISVSGHSAGGHLTGMLLS
                     TDWEKNYGLTPNLIKGFLPVSGLFDLKPFPFSWLQPKLLLTSEQVLRNSPVFLKPVYT
                     PHVMVAVGADESHEFQRQSKNYTIFLQKHGVPAEYLSMPGKNHFNIIHDFLGDGGPLC
                     KKIIEWK"
     gene            complement(24517..25185)
                     /gene="tsaB"
                     /locus_tag="EYO68_02255"
     CDS             complement(24517..25185)
                     /gene="tsaB"
                     /locus_tag="EYO68_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIC06656.1"
                     /translation="MIRLSVDTSFEHLGLCLTRNGVPLANYYSLCHRRNATIIFEVID
                     DLLKSAELSLAEVDAYVVNCGPGSYTGVRIGMSVAKTFAQVHDKPVIPVNSLELLAGQ
                     IRHLKNDFPVLLNCTRREIFHSVFRIVDDHPVAQTAIQLTSLEKFIEISGGVPVVHYR
                     VNPERRKHEPLFEQLVLLPLDYPVPDAQLLDRLGAYKLASSAFDPDLPVQPLYIKRDV
                     EKPL"
     gene            complement(25239..25700)
                     /gene="tsaE"
                     /locus_tag="EYO68_02260"
     CDS             complement(25239..25700)
                     /gene="tsaE"
                     /locus_tag="EYO68_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIC06657.1"
                     /translation="MSKLILELNSLEITDVVARKTAAHLFPGAVLLFWGNMGAGKTTF
                     AKSLCAGLGIPHETVISPTYTMVNIYSGKWTILHVDLFRLTAPEQLDDFDREDLFADD
                     GITLVEWPEFITDHLTDEPVLNLYFKTVSEYQRQLIIKSEFDDFDTLLKTL"
     gene            25710..26402
                     /locus_tag="EYO68_02265"
     CDS             25710..26402
                     /locus_tag="EYO68_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06658.1"
                     /translation="MNPRVLISCHNLGYAAPSGTGISTVEKWDIRKLNLELHAGEKAC
                     FHFQNEEQKQVLWRMFQQKLKPKTGSLQISANTHLHTDDSLWEGTDKKASLLENMESK
                     LFSTRPWFGGQRKNMDILKDRLGLTGRIINLPVNELMPEYAARFWALMLVAANTKVVL
                     INRLLSQLDEISLPFIQEWQESFSGITVIFGEHPEYFKAVNSRKVEQQDTLKSFFSTN
                     ISFSADGLAKHF"
     gene            26507..28276
                     /locus_tag="EYO68_02270"
     CDS             26507..28276
                     /locus_tag="EYO68_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIC06659.1"
                     /translation="MQFSKSFIRQVADATDLVDLISDHGVTLKRAGSSYKGLCPFHSE
                     KTPSFNVNPERGFFHCFGCSVSGDGIKFLMQYDRLSFSEAVEELAKRAGIPLEIQSGG
                     PRSSNPEEDRGLHCLKEAAAFYSEKLSIPEGENAVKYLQQREVPESMWKQFQIGMSPD
                     EWQGTLNYLQKINISLAEMSRTGLIKVSEKSARHYDTFRGRLMFPVKDVRGRCIGFGA
                     RAMKPEDKPKYLNSPETSYYRKSQVLYGLHEGLTSIRKKRRLIFVEGYLDVIRLHENG
                     FTEAVAPCGTALTQEHFKIALRYADTVILLFDGDSAGNNAALRHAHLLLPVALESYIL
                     TLPEGEDPDTFLLNNGKENFDAFLNQKVPALDYLVQQTIKKYPDSIQGRMQGLDELLP
                     ALYEIQDPKRRQLTLMTIGERMKIPPELLSKELKRKENKSLHKNKNNDIVSCLSENNK
                     DSQDEQWLLQSLLRKGKLWPRVREHLKPEEFQTPNLRRLYEKLLQLPDAVFQAFDPFT
                     LEKSDPDLFQSLMLLLSEEIPSHDFGLSLLRMKERNLEIEYQEWLLHSKSNEERAQAA
                     LKRRLEEKNLKEIKQIIDNILTQ"
     gene            28293..30083
                     /gene="rpoD"
                     /locus_tag="EYO68_02275"
     CDS             28293..30083
                     /gene="rpoD"
                     /locus_tag="EYO68_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIC06660.1"
                     /translation="MNTANKSDNEFSSVEKKLIKELVSDGREKGFVTIDEINESLGDE
                     TPSPEQLEKIFNIFAEMDIEVIDSEKKMDIVSDQTDSATELDNETGELNLDPGIDNST
                     DDPVRMYLREMGSIQLLSRDGEVEVAKRIEKGNADLIDIFSDSKLILLYLQNLTKHLK
                     NKKIKARHVISGLDEDDNVIEDEDKATVKLIAQFDVLWKHHRSVNKFQKHQAKRKLQP
                     AEKKRLEESESLFKDAIRNISFNSRQIELMCDEILNHKEKINLTFRKMNSNIKSLDLP
                     VEKMDKLFEQLSSATSKKAKEKTAKDISAKTGHPFSIANRYFEKIELKRKRISTMIEL
                     TTLSLDDFNLEVKSLKQVERKVKSAKSQLVEANLRLVISIAKKYTNRGLQFLDLIQEG
                     NIGLMKAVDKFEYRRGYKFSTYATWWIRQSITRAIADQARTIRIPVHMIETINKLSRI
                     SRAKVQELGREPTADELSEDLGMPLYKVRKVLKIAKEPISLDTPIGDDEDSQLKDFIA
                     DEHIRDPGEATVSSNMGERTREALKTLTKREENVLRLRFGIDSIKDHTLEEVGQDFDV
                     TRERIRQIEAKALRKLRHPTRSKLLKSFYE"
     gene            30335..>33203
                     /locus_tag="EYO68_02280"
     CDS             30335..>33203
                     /locus_tag="EYO68_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC06661.1"
                     /translation="MRLFRNFLILLIISCSTAAGIPAQTSKTSDPVIIQNVELLRHGR
                     QISVRLLTDNPPVYVITENLASRTLVIKFNNARIGFSDGRNERLFNDPQLAGIRFSAI
                     DGDSWAQFKLLKKDLSYSVSSLTEQNGIQIDFRPALQIEPLPDPPEDAAYQLVSIKFD
                     AGNTDFTRLTFGFTKPAKFSATGSVPEDRQPPRIFLLENSLQTQLTIRLPNTLPEKQL
                     LNLDYKDARLELHALTSEVNQTFIELKLNATDVSVERHFVSRPVQWILDIHGTPLKGK
                     IAADLTEGKKLSPEDAAAFEKEEKERRIRASKINPIFQRGENAFRKNLYDEAVSHYKE
                     AYALGKEHTGDEFEDPLHPLSARALFRIGDTIYTMLERRIGDNYHQAIEAYKTAIRIT
                     QDAEKIAKENGEEFETAYLLPHANFRIGRSYQKMKFQHEATVYYKILQERFQNSIEAM
                     EASFWKGMSRIEQRQWEMAINEFKEYLRTSPEPKFFAVTHYKMAQAYYQLKRYITAKE
                     FFDIARNAEEDYAKDDPTLLFHMGETYYENADYVTAREIFRLLLEKYPKADFSKLVAL
                     RLGDFLRDEGKENDAILAYKNAISSYTREIALLGKLRIANIQAKRPYSDEHLEAITVY
                     DEIHRLYPETPQAKEALLRKGLTLTLYGYYARAIKTLESFMEKYPQNVYVRRNVIQEN
                     IDENLKGLIDQYFRQDDTLALVSAYSDYKAKYLFNFRFDTTLFQTAVAHRKLGFYEET
                     LDILRFLETRSGGTIGELVQLEKALTLLEKEDLTEARNITAGFLQKYPASPYDAEARQ
                     LLAKIYRRKKSYPNALLVYNQTLQKYDQNKDPLSAEIVPELYYDLGELHEETGNFVDA
                     GEAFQEAVSSYNHPLDHPDTPEYIINSHFLAADMYNKAQNDTIALLSYQQAISLYADS
                     ENKEINERVFWARYQIGSIYARQENNEQALKIFKELVDHKDG"
BASE COUNT        10491 a         6730 c         6632 g         9350 t
ORIGIN      
        1 tgctgaaatt acaacaatta ataatcgatg caaaaagttt ttccgcaagg caaactataa
       61 aactacagag cagtattatc accactttaa aagaggctat cttttttgca tatttaacta
      121 tcaaaaatgc cagttacaat atcaaaaata ttttagataa atttctctaa cattttgtgt
      181 agatgtttcc actgtgtcac gcaaatttga gacttcgtag ccatgatgtt tcagtagttt
      241 gagtatagtg tcttcctgaa ctttttcaaa atcaagcaaa tccgttgatt cgtcatagat
      301 caaccgcagt ccattttcta ttagtctcaa cattttgtaa ttatattgca aacttttggc
      361 ttcttctttt tttagcaaag ccagttttcc aagctcaggc agcacttcca tggttttagg
      421 gttctgcaat tgaggaaaac tgctgccaaa tttcaactga agtgcctgcg tcagatattc
      481 aatatccaat aatcctcctt taccttcttt gatgttacga cgattatctt tttctcctga
      541 cagttccagt tctttgcgcc gccgtaaata atttatttgt gcattcatat cagacggagg
      601 aatccaatca tatacagcac tgcgtattcc ttcttccact ttttgatacc attctacttc
      661 accactccct ccaataaccc gccctttgat cagcacctga tgttcccagg gttgtgatgt
      721 ttcatggtat ttgagatact gatttaaatg tgtcaccaaa acaccagctt tcccggatgg
      781 acgaaggcgc gcatcaagtt tataggcata accggaacta gtaatagtag aaaggcagga
      841 aattaagcgt tgaatcaatt ttacccaata ttcctgtgct ccaatgactc gtggaccact
      901 tgtagttccc tcgccggaat aaataaaaat caagtcgaga tctgagtgat aggttaattc
      961 tcttcctccc agtttaccta aacctataat tgcaaactgt ccggattcat ccatggaatt
     1021 caggaccttg ccataacgtt gagttaacca tttttgtgac aaacgaaaag cggcttgcac
     1081 aataacttcc gctagtaaag tcagggctat tctggttcgt tcgtaggaaa ggacgccttc
     1141 catttccgca cttccaagca gaaacgtagt agtgtgcttg aattgtctta actgatcaat
     1201 ttcttcttct tcattttcag cgcagctttg gatattttct tcgagttgat ctgtccattt
     1261 ttctactgat atttccattg tataattgtc cagttgttct aataattcca ggtgattaag
     1321 caaataattc caaagcattt cgctggtttc agcaattcta gtcaagcgtg aaagcgcaga
     1381 aggatgcaca gacaatttgc gatagcaacc aattttcccc ttgatggatt caaacaaacg
     1441 ctgaaagcga ttttttgtac tctcactcat aagcggggcc agttgctgtg agaaagattc
     1501 aataaaccgc tgctcctctt cagtaaagtt ttgaatacgt gcacagttgt ggatagcagc
     1561 ttcaatttcc aggtgtttac tgctgaataa tccactaaaa atagcacgaa ctccacccat
     1621 tacatctttc aaatcactca gaaagtgttg atgggctttt tctatgtcaa attcgttgta
     1681 acctaaattt cttgcaattg catgttgttg tacaatgtct gaagctaaag tgtgagtttg
     1741 ttgttcctca cgcagttgaa gatgatgctc cactctacgc aagaagaaat atgctttttc
     1801 cagagtatct gcatcctgtt tcggtattaa ctctgattga cgcaattgtt gcaaaacctg
     1861 caatgttcct gctaggcgaa gttgtttgtt tccgccacca tacaaaagtt gaaaagtctg
     1921 cacaaaaaat tcaatttcac gaattcctcc tatacctaat tttacattca aaaaactttc
     1981 acgcagatgt tcctcttcga tacgcttctt cactttttcc acatcacgca atactccttc
     2041 gtccagcaaa ctacgaaaaa cgaaaggggt gatcatattc attacatctt ttcctaactg
     2101 ttttgttcct gctactggta ctgctttgat tagagattgg cgctcccata actccccact
     2161 tacagtataa taatattcca tctcatctaa ggactgaacc aaaggtgcac gctctcctcc
     2221 tgggcgcaat cgcatatcca tgcgagcgag aaacccttcg tcagtgacct ctgacattac
     2281 ctctatcaat attcttgctg ctttcatccg tagtttataa tcacgttccg tatctccagt
     2341 gagaggttcg ttgtcatgta tgaaaatcaa atcaagatca gatgagtaat ttagttcgtt
     2401 tccgcccaat ttacccatac ccaaaatcat aaatgggaac agctgttgag gagactcctt
     2461 tgaagattga gaagattctc gatttgttgc tacagatgaa tccggaattg aactggaaat
     2521 tgaatagtta gaaatttcca gttcgtgatt cgtaattcct gatattgcta cccgcaaaca
     2581 acaaattgcg atcgctgaga gttcctccaa agtttcttta aatggacata gttgtgccaa
     2641 atcacgaact gttatccgta gaaattcttc atatttgtaa cgtcgtaaaa tatttttaaa
     2701 ctcaagcaaa gaatactcgg tctgcaaatt cagacgcgtc cggagttcat tttccatcac
     2761 ttttaaatcc ttttttcgta aaagaaaagg ggactccaat aatcctacag caagttgagg
     2821 atttcgtttt aagcgatttg cgagaaaatt actgcgcccg aatatgtaca gcaaagctcc
     2881 tgtgtcaggg cggctccaat caaaattact tgagaacttg ttccggaaag catctgcaaa
     2941 atcatgcagc tgggtcaaag ctgcttcagg tgttccggag gaaagcaaaa tagggaggta
     3001 ctgagcaagc tgagtttcat tttcgaaaat agctaagctt ccttccaaca ggtccacaag
     3061 acgttttgca gattcggaag tgaatggtgc agaagattcc tgtaaaatgg ctttccagtc
     3121 cttgtccttt gacaatggaa agagatttgt aacccagtcg ttgagtgtca cgttaaattt
     3181 atttttggtt ttatgtgtga gcacagttta gcataatcag gtactcgtca gaaataaaaa
     3241 acacctcttt ggtacaagct aatgtccgat agaaacaaaa cttagagacg ctcgcttcaa
     3301 ggatctgcat ccgtattaag ttggcggtgc tacctgtact caagcaaatt ttctattttg
     3361 tcatttgcct ttgatccgaa ttcgagattc agaacaaggt cttattggga ggaaaaatga
     3421 atggcaataa tattgaggtt cgtgggagcg aaagaaaact cccacaaaat aaacagatta
     3481 atagtggtaa tacaactcga actcatgtgg atgtggacgt gagtcaatgg ccctgacttc
     3541 ttccattttc cagtccaacc agtattcaat cacatctttc gtaaaaactt cacctttgag
     3601 taagaattca tggtcattct tcaaagcatc cagagcttca ttgagcgaac tcggcatctt
     3661 accgattttg ttcagttttt ccggtggaag atcataaata tttacatcca tcggctctcc
     3721 cggatcaatt ttattttgaa ctccatccag tccggccatc attattgcag gaaaggcaaa
     3781 ataggcattg gctcctgaat ccggacaacg gtactcaatc cgtcggcctt ttggtgaatc
     3841 tgcaactgga atcctgatag ttgccgagcg gttacggtta gaatatgcca gatttattgg
     3901 tgcttcaaaa ccgggtacta gacgtttgta agagtttgtt aatgggtttg tgaacgcagc
     3961 taaagcagga gcatgtttga gaatacctcc gatgtagtat aaagccattt cactcaaccc
     4021 tgcatactga tctcctgcaa acaagggttg tccatccttc caaatcgact gatgcgtatg
     4081 cattccactc ccgttatccg caaacatagg tttcggcatg aaagtggcgg ttttgccata
     4141 ttcttgagca acattacgca ctacgtattt gaaatgctgc agcatgtcag ctgtcttcag
     4201 catagtgtca aatttaaaat caatttcatg ttgtgcagga gctacctcat ggtgttgcgc
     4261 ttctattgtc agacccagtt cctgcatcgc caaaaccatc tccgctctta tgtcacggtc
     4321 ctgatcaaaa ggagccgccg ggaaataacc tgacttgtgt ccaattttgt aagctttgtt
     4381 tccaccaact tcttcaacac ctgtgttcca tggacattcc ggagaatcaa ttgaatagaa
     4441 tccgccttcc gggccatatg cgtatcttac gtcatcaaac ataaaaaatt ctgcttccgg
     4501 acctaagaaa attgtgtctc cgattcctgt tgaaatcaga taattttctg cttttttagt
     4561 aacactgcgc gggtcacgag tgtaaccttc accggtaaca ggatctttaa tatcacaaat
     4621 gatagacatt gtcgcaactt catagaatgg atccattttt acagtatcca aatccggcac
     4681 aactttcatg tcgctattgt caatggtctg ccaacaacga atactggagc catcgaacat
     4741 tcgaccttca tccagcatct cttcattgag ttctgaaact ggtactgtaa aatgctgcca
     4801 tgttcccaga aaatcgatga atttcaaatc caccatttca acatcatttt gttttgcaaa
     4861 ttcaacaatc tctttcggcg tagtcataac ccaatccttc ctttctataa attaaaaaaa
     4921 ttaaccgcaa tcatacggtt taaaaataat ataacctgtc actagaggca aataccaagc
     4981 cacaaatcat ctcactgata ttgtatattt tttaatatat tattgcaatt agatttgcat
     5041 taattttagg caactgcata aaatttaatc aaatagtatt atcaattgtg agctgagcat
     5101 tcggttagaa tataaagttc catcaactct aaagcaaaaa tcagcagccg ttctggtata
     5161 ttgcttgctt tgatcaaagc agctaaaagt tcattaaaaa ccaaaatcta caatattcgg
     5221 ataccttatg agtggcatta ccagccttgt tcaggcggca ggtgttatcg aacataaaat
     5281 ggaaactgtt tcaaacaatc tggcaaacgt caacacagtc ggtttcaagg aagatcagcc
     5341 gtctttcagg gaggttcttt ccaacgtcca acgcattgct ccggaatcac tggaagagcg
     5401 tttcttgtct catgaatatc ttgatgacta tgtaggaatg gacaaatccg cagtcgttgt
     5461 agatgaggta gggaagaatt ttgaacccgg acgaatgcgt tctacaggaa acaatcttga
     5521 ttttgcaatt tcaaacgaag gttttttcac aatttctact cctcagggtg agcgttttac
     5581 ccgggccggc aattttcaac tcgattcgac aggtcgtatt gtcacaaatg acggttatcc
     5641 ggttttgggg gaaaatggcg taatcaccat taaagaaggt tcaattgatg tcagcgaaag
     5701 cggactactt agcgtcgatg gaataattgc tgacagtttc cgtctggtac gtttccgtaa
     5761 tcaggaccaa ttgcagaaac tgggacaggg attttttgcc cctgtcaaca gcaatgattt
     5821 gccggttgct tcagatggaa tcaaactcag acagggtatg ctggaagatt caaatgtgaa
     5881 cagcatgctc gaaatgaccc gtatgataac tgcaacacga gcctatgaat cggtacacaa
     5941 ggcgctttca agaatcgaca gattagatga gaaagcaatc agcctggttc agtcataatt
     6001 tgacatttac gaaaatataa acctaattat ttaatatact gatacaggag tttttcgctt
     6061 atgatgcgtg cactttatac agctgcaacc ggaatgcagg cccaacagag aaacatcgat
     6121 acaactgcca acaacctggc aaacgtcaac accacaggtt ttaaaaagag taaggtccag
     6181 tttcaggatt tactgtatta caatgaacgc agcgccgggg ttccatcttc agtttccacc
     6241 caagttccgg taggaataca tgtaggacat ggtgttaagt acgtcagcat tgataaggta
     6301 catacacagg gcaacatgga aaacaccggt aatgacctgg atttgagtat tgacggaatg
     6361 ggattttttc aaattctcca accaaatgga atcatcgcat attccagaga cggccatttc
     6421 aatatagacg gaatggggcg cttggtgaca aatgacggaa tgctgatgga tccagaaatt
     6481 aatattcctg cagataccag aaaagttcaa atcggtttag atggaactgt atcaatattt
     6541 ttgcgagatg aaaacttgcc tgaggcactt ggcagcattc agttagcacg ctttcagaat
     6601 ccaacaggcc tgactccaat tggtaaaaat ctctttcttt caactcctgc atcaggaaat
     6661 gcaatcgttg atgctcctgg atcaagtaac atgggatctc tcacacaggg atttttagaa
     6721 cgttcaaatg tgtcccttgt tgaagaaatg gtgaatatga ttactgccca gcgggcttat
     6781 gaggtgaact ccaaggctat ccagtctgct gatgaaatgc ttaaaaatac taacaatctg
     6841 atcaggtaat taattaactg ccaggcaaat ttggataaag caaaagtata acaatagttt
     6901 ttccctctac gtacttaagg attcgcataa atataatgcc gtcattagcg cacttaatta
     6961 ttaaaacaaa aggtatagca atgagacctt atccgtatcg ttacggacta ttgttgctgt
     7021 tatttttatt atattccgga acagtatttt ccaaagggaa tactgcccct gaagcacagc
     7081 aaatcaaaat tttcctgcca caatcttcag tgattaacag cgatgaatat cgacttggtg
     7141 aaattgccca gctagaaggg gaagatttca tactgttgga cagactggcc aaagtcgtca
     7201 taggacgtgc acctttgcca ggaaggaaat taaccgttac ccgttctctg attctctccc
     7261 gcttgcgctc ccacaaagtc aacattaaga aattcgtttt tcctggatca gactcaacaa
     7321 gcattcagcg ggcagcactc aaaatatctg gcaatgatat agagcaggta gtattgaatc
     7381 acattaaaga aacaaaaggt ttttctgact tgaaacctcg tctgctggca aagataaggg
     7441 atatttttct accacgcgga caggtaagct atgtaatttc ctcaaaagga aattacaaaa
     7501 aagaaggcgg ttaccgtaat tatgaggtcg agtttagtgt tgatggcaaa gctatcagga
     7561 aagtctcagt acggacatat ttaaaaattt acaaggaagt gtatgttgca cgcaatacta
     7621 tcaaacgtaa ccatataatt gaagaatcgg atttgttgaa aattcgcaaa aatgtggacc
     7681 gcatgccgcg tgaatatgta acagacaaag aacagctaat tggcaaaata tcaacacgta
     7741 caatcactcc aagcgaagcg attcagggaa acacactttc agatccgccg ctggtgaaat
     7801 ccggagatcg tttgcaaatt gtcttcgaaa caccgtttat acgtctttct gcacctggaa
     7861 tttcattggc caaaggccga aaaggtgaac gaattccggt gaaaaacgca gacagtaaaa
     7921 tcgtggtttt tgcgactgtc aaaaccagaa atatcgtgtt ggtaaattaa attgatatgg
     7981 tttctaaact agaattttca caattttaat cagtgtataa attattaatt ccattttcag
     8041 gtttatttca ttggtttctg gccacatttc tggttctgag cctcataggt tgcgttccgt
     8101 caagaccaca accgaaacct cctgctgagg ttttggccgc ttatgaagta aaaaaaatga
     8161 atgacaattt aaaacggact gcaaagcgct tacggcgtaa gcgtatttct acaagcagaa
     8221 accgctatga aggttccctc tggcgatatg aatcctcatt tggtaatttg ctaagggatc
     8281 atcgtgcacg tttccgcgga gatttgttga gtatcaacga aatgagttta atagtgaatg
     8341 tgcctccacc caaaacaccg gcagagggac aacctgccgg aggtgcagaa acagcgaacg
     8401 ttgctcttga agctctgact ctcagagatt caattgaaga agaacaaaat caaattttgc
     8461 gaaatctgga tggcatttct gcacgtgtca ttcgtgtact tcccgatggt aacatgctta
     8521 tcaaaggtca aaaaatcgat tatcgccagc gtaatcaagt ccgttatatt acgactgtta
     8581 ccgggatttt acgtcctgca gacgtaaatg attcaaacgt tgtttctgcc agtaaacttg
     8641 caaatccaaa cgttaaaatc aagcgtcagc aaagcggaag cctcgtccgc gagcgcctcc
     8701 aaaaacttgc accattgctg ggcaagcaaa aagccggatt cttaggtcgc ctaggtgata
     8761 tggctaaagg cggtgcacag taaagaggaa ggaatttctc tgatattctc cgcagtcttt
     8821 tcagcatcta caggtttgtc caaagctgat ctactcccaa aacacctcaa taatttctca
     8881 agtcattccc atacggaata acaaatatat actcaaagaa attttcctct tgactccatg
     8941 tcaagggaat gggggttttt gaatgcttga aaccctatac ttttcttaac ctttttcatt
     9001 attgtctgcc ccaatgtgaa actgttatta cttgaaattt gcattcagat ttttcaatgg
     9061 ctttgttata caagtaggtg acaattcact ttattattca gatcacattg cttaactgat
     9121 tatatgacaa cagttacaaa caccgatgcg ccctcccaac tagaccaaca actggaactt
     9181 ctgtgctcgt ttaatgtaca gattccttgt aatccccaag gagaatttgc tgcaagcagc
     9241 tttaaaacac tgttgcaatc actcaataca aatcaaatct gtgattcctt gcgtggatcc
     9301 taccacgatg tgcatttaaa gaaatggaaa gaatacgcac aaagggaatt tgatgagatg
     9361 ggtcgaataa accgcttacg tttagaaagc ctaatgcagc tttctgatca agaaatgcac
     9421 aagacaattt ttgaagggat tctccttttt gacatcaatc ccgaaaacgt tgccccgttg
     9481 gagttacagg aaaagactgg tgagttcgat gaagaaggga atccagtaat gagtactatg
     9541 accttcgatg tttttcagaa gggggcaatt catggaattg aaggactaga aagattttta
     9601 ccttctgcct caattaaggg agaagcagga atggatgcac atcttgagga ggaattttcc
     9661 ggaacagacc tcatgagcaa tttcaaacag gaatccgggc agttgatcaa gtcactgact
     9721 accataggga gtttgggtgg aatcggacac aaacctgatt ctgatatgga tgcacaaatc
     9781 attattaatt ccaatccgga attcaaattc agctggaatg atgcagattt tcttgtcgca
     9841 ctaattgcaa atgtaatgga gtctttttat gacgactatt acattaatgg cctcactact
     9901 caagaacgac tggtaataaa aaaagcggct gccggaactt taagagagca gtgcagtaca
     9961 gggttaagcg aagaagagca acaagtgata gaatttatat ttgccagcag ctaccgcaaa
    10021 gagttaagga aactgattca agaacatata caaaaacgac ccgcagaaga gcaaaaacaa
    10081 ttcttccaaa aatcagtgat ttcaacttta aataaatatc ctgattgtga aaattttctg
    10141 gagccgctta aaaagttttt ctcatttttg aaaataggag gaggagattt acagcaaaaa
    10201 gccttcccct attccttaaa gcaattgagt aaagaaaagg ttttaaattg tttgacaaat
    10261 tattaccgta caacttttct ggatgaggct ggtgcccgtc aaattttgtg gcgttatgga
    10321 gtcaataaca atcttgcacc tgaatctctt ccggaggaaa aaaagaacga atgttttttg
    10381 aacagcttaa caaataattc tcagctttca acactcttga ctgagttttt tgaatatctt
    10441 tcttctcatg ttgcatatgc ttctatgaac aaactttcag aagcaatgca aacactcaaa
    10501 caacacttca gttctcataa tgttgtattt aaggatggac tggaacagca ggtattgtca
    10561 aaactggaaa taaattacag ttccaggact gttaggatga ttgaaacttt ttcaaacgga
    10621 caagccaaag atctcgaagc tgaaattgaa tatccgttac atctcaaaat ccagcaggca
    10681 gaagcatacc tgactaaaaa atatccgact accaaaattc atttttttac taatattttg
    10741 cggaagcagc gcgccggtca gcacacacca tttttggttt ctccagaggg aagtatggcc
    10801 tatgcactga tgcttaacga ttttcttctc aaccctgctg ctatgatctg tggtattaca
    10861 ccaatgcctt ttgacctgcc gaaaaatttt aaaattctct cctcaattgg agtttttcct
    10921 gaagcagaat ggaccctgaa acaaaacctt gctgctgaat atagaaaaaa taataaagtt
    10981 actgaaaatg atacaggtga agtacagatt aataagaatg ttactgaaaa gaaccaaata
    11041 ttggaggaag agaaagaatc attcatcttg ggaaagctac caaactgggg tgaaataatc
    11101 attccgcgag aaatgttttt ggggcacgca attcccatat ttttgaggga aagtgagaaa
    11161 ataagtcgcc gtaaccttcc caaagctctt ctcaattgct ggtggctgga aatgattgtc
    11221 tgtattgatg atgaagatga actgcctaca agtttgacca gattgttatg gaatccggaa
    11281 ggacgttact ttattcgaga aaatcgaaaa gggcctctga ttgatgcaat tgttaggatg
    11341 gaagacgact accctgcttt acagcttgac ccgtggtggc ttaaatttac tgaaatgctg
    11401 gtacgttttg aaagctatga acaagaggag gaagaagaac cggattttga actcaacacg
    11461 ctgtctgaaa ctcaaaaaaa tattgttttt tgctttgcac agcacatgcg aatttctgat
    11521 gttattaatt ttggagatga cggaaaccct gtatggttag acgaaaattc aacctggcgc
    11581 agcagagcat tagttgattt ttataaaatt ttcttctcca ttcccgaaga ccgtcgtgaa
    11641 ttaattcgat tttcagaagg gcgggatgac gcaggaaaca aaatggaaaa aatgctgaag
    11701 aagctttttc ttgagtcaat gactcgagtt gagaataaac tttgtaaaat tggacataca
    11761 cgcgctctga ctcaaatatc caatcaactg gcacgtctga gtgagaaagg atttgagaaa
    11821 gaaaaagcag ccaacattct cagccctttg ctcgatgttg taaatcaacg tgtttcaatt
    11881 gaggacagga aggtactggt aaaacttaaa aagaaaatcc cactcaacaa aattgaacaa
    11941 atgcaggcta agattgttta tgaggaatta caaaaactaa aaagcgtaca aggcaacatt
    12001 gttgattatt ttaaacagta tgacttaata atgaaggaaa gctgggtcag aaaaaccatc
    12061 acaaatgcta aggtaagtgt tgcaggcgac ccactggaaa atgtgatttt taaattccat
    12121 tttgaaagaa actttgaaag aaaaccattt caggtactgt tacctatttc aaaaagtttg
    12181 agtattcctc gatcaagaat aaaggttgag tttgtacgaa aaagtgaaaa atggcaattc
    12241 agttccatgt tgagtcgtaa agaagcaggt ggagggaaaa gtggtgcaga aacagttatt
    12301 ccgatgtttg aagaaaatct ggtggaaggt attgcacgtt gtacttttag cggttacgtt
    12361 ggctttggag gcaaatatct ttctactttt gaaaaacctg ccgcacaggt tcatagtgat
    12421 gttgcaatga atccagtttc gggtggtgca ttgttcacat tggctactga aataatatca
    12481 tttttctcgc atttttctgt ttcatcacgg gaactgatgg aaaacattca ttacatacga
    12541 gatgttttga tggtgtgtaa tgtcaacaag ctcaatatta tttcactaat cgtacgagac
    12601 aatcttggag agcaatttgt tatcgctttt gatattagac aaattgtaat caaaaaggtt
    12661 ccacctaagt taagaattgg tggagattct gccttggctg aattttttat gcgtctcaat
    12721 agtcgggagt gccggatttt attcatgcac catttatctg ctctaaatat tccaattcga
    12781 gcatcacacc tgcctcgttt gcggatttgg gtcaatggag caaattataa acttcctatt
    12841 actccaaaat ttcaacagaa ctatctcaac gggattgcca acacactctg gcctaatgat
    12901 tcaattggta caagagaaca tctgctgcca cctccactga caagaacatt tgatcagatc
    12961 ggcagagcat cgctccaagg ctaatttcat tttacctgat gcgggtggca agaaaaagaa
    13021 cagagaaata agttccctta tttaacctat cttcgtataa tgacattgtc agatggtttg
    13081 atatctttat aaccaagttc cttagctaaa tcatggcctc aaaagaacca atagaaaatc
    13141 caaatacacc aaatgttaat ttcatcggtt ttcaaaaaca acttcgtgaa attatcaaag
    13201 atattaaaca atctaataaa aaaactgggc taaaaaaaat attcggtggt aaaaaacagg
    13261 taaacccgca tgttgatgga aggattctgg agaatgatgc gccaaataac aaagaggtct
    13321 caaagctaat caaacaatta gatagtgacc ctaccgaccc tctggttcgt ttgcgcctgg
    13381 tcaatgctat gatggcagac agaaaagttc atcaccttca aactcatttg aacatgatgc
    13441 ttcaggcttc aataccaata tatcttagtg atataactac aacctttctg cagttggtta
    13501 tgcatactta tcgtgcttat ctggagcgtt tggcaaatat tcacaaacac aacatgatgg
    13561 ccatcaggtc agaagttctg aaaaatgtga atatgagtgg tatagatgta gaagacgaag
    13621 atacggaaga ttcccatgtg aaagatactg aatctatgaa aaccgaaatc caaattgcag
    13681 aggccttgat agataattgt gaaacgataa ttcaaagagt aaaaaccaag atgacttcaa
    13741 cgctttcaca cgaagaaatt gaagagctga catcaaaaga aaaagcgtcc tcttcatttt
    13801 ttagtggcga atcagaaaaa gtaaatccaa ataagcagaa tatgatcatc agcaaagcag
    13861 tacaggcaat tgaaatgcta aaacagattc cgttactcca tggagcagga cttgacttgg
    13921 cacaacaatt gggtcgaatt gacaacaagt tgacatatcc tctggtgatg gaggggcgaa
    13981 ttcaaatgca agctctaaaa tatcaatttt tgcgaattga aagcggtgac cgaagtgccc
    14041 gagaaaacat ggctccggta tacaatcttg cagttgtcgc ttaccgcaaa gcgctgaaac
    14101 ttacatcgaa aactactcca aaaaaatcag atcttcctgt tcttacagag tttgggaatt
    14161 taacccatta tggttttata cacagggatt taatgcgctt taccgaagat ggtgtaatga
    14221 atttggtaaa attaggaaaa gattccgtgg atgctgctgt gactgttgat gaaagttttg
    14281 tcccactcca aaaacgttta gagagctcac tggctcagtt gaataaagtc gagggtgaac
    14341 acacttaaaa gttcgcttta aataagcttg acctgaaact tcatgtggag agtcttcttc
    14401 aaaaaattca aaaaaactgg gaaacttacc aactggcagt tccagaaaac aaacagaaaa
    14461 acagaaactc tcaaaaaggc ccccttgagg ttatcgtctg tgttagtgga ggaagcgact
    14521 ctgttgctct gttcaatttg ctccagcgcc tcagcacact tcttcgattg aaattacatg
    14581 ttctgcactt caaccaccag ttgcgtccgg aagcacaaca ggaacaagat ttcgtcgagt
    14641 cacttgccaa actgcataaa ataccctttc attataaaac agcaaaacac ctgaagcatg
    14701 gacagcctgg gctacaggag tcggcaagaa actggaggat tgaagaatcc tgtcttttgc
    14761 ttgaatcttt agggggtggc tgtattgcta caggacatca tgccgatgac caaactgaaa
    14821 cttttctact aaaatggctc cggggatcac atatttccaa tttacaggga atgcggtgga
    14881 aaaacactcc tttcatccga cctttactca actgtagcaa attggaactg caggtttttt
    14941 taaaaaataa taatctgact tggatggaag attcttccaa tcaaagtaat gcatacttac
    15001 gtaaccgggt acgtctggag ttagtcccgc ttttggagga attaagccgg caggggcttc
    15061 aatcccggat tagtgatatg agtggacaaa gcaaacttct tcgagagtgg ctggattcac
    15121 agtatgaaaa atggagactt cactgccaaa ataacaaaag cgaggctccg gaagtcttgt
    15181 ctttaactga tttagagaaa gcaaatgatt tacttcagca ggaaatcatg cataatttta
    15241 tcaattctca aacagaactg aaattgagtt acgccaaact ccagaatatt tttgaattaa
    15301 ttcatacaga caacaataac tggcaatttc gcctgtcaaa aaaatggagc attttcagat
    15361 ctgttaataa tctggaattg catttgaact ctgaatcata agaaaactga tcatcaacta
    15421 tataaaattc actaggcagg aataaattca ttatctcaag tccaaatact agaaataata
    15481 aaaaggaata aaatgcgaat cggcattgac atgggtggaa ctaaaattga gggtatcgcg
    15541 cttgctgata caggagaaga gttgatccgt aaacgaattg acaccccaag acatgactac
    15601 gatgaaactg ttaacgctat tgctggaatt gtccttaacc tggaatcaga aactaaacaa
    15661 aaaggtacag ttggtgtcgg tattcccgga gcaatttctc cgcaaaccgg actagttaaa
    15721 aacgcaaatt ccacctggtt aatcggaaag catttcgacc tggatttgga gtccgcactt
    15781 aagcgtgaag tacgcctcgc aaatgatgca aattgtctcg ctgtttctga agcagtagac
    15841 ggtgcaggtg caggttcaaa agtcgttttc gctatcatac ttggtactgg ctgcggcggg
    15901 ggaatagcaa ttgatcagga ggttcatacc ggacatcaag caattgcagg tgagtgggga
    15961 cacatatctt tgggctggat gagaccggaa gaatatcctg gcactgactg ttattgtggt
    16021 cagcgggggt gtctggaaac atttatttcc ggaactggat ttgaaaatga atacgagctg
    16081 caaactggaa taaacaaaaa tggtcgtgaa atcgttgaaa tgctggaaca tggtgacgct
    16141 gctgctgaaa aagttatgca gatttatgaa aacagacttg cccgagcaat tgcaacagca
    16201 gtaaattgtt tagatccgga tgttctagtg ctcggcggag gaatttcaaa tatttcacgg
    16261 atatataaaa ccgttccgca attagtacaa caatggactt ttggaggaga attttccaca
    16321 ccgatccttc ctgcaaaaca tggagattca agtggagttc gtggtgcagc ttggctttgg
    16381 taatcaatta ttccgggatt tttcgactaa ataggcaaaa ccaaccactt cataagtcaa
    16441 tgacagcggc aaaaacaacc agccaataaa cggaataaaa gcgagaaacg ccggcagcca
    16501 gcctattcta atcggcatcc agcgccacat cttccggagt tctacggctt cctcctgatt
    16561 tgtcaaacta tctaaataaa cgatgacacc ttcccgtccc atcagatgag attcaagcat
    16621 aaaaacaatt gggagaccta taaccggaat caaaccgaca aaaattagca atactgggaa
    16681 cagacaggct ttaagtgctt ctttcaaaat atgatttatt tcagctaacc aagctttaaa
    16741 agaaaacaca ggttcttcaa tttgtctgaa atgtgttata atccgctgta ctaaactttg
    16801 gtgccagaga ctaagcagtt caacagaaaa tcgtaaggat accatggcaa taacagctgc
    16861 gacagtaagt tgaatactcc acaaaactat tgtaccgaca gcagcccaaa acccgtctcc
    16921 accaaaaagc cagttgataa acggaccgag aagttgaaaa taaaacaaac cgttcaacgc
    16981 cagtgaaact gcaaaaaaga tgagataatt aaaaaacatt cctcgaacag ccatgccgcg
    17041 caggccttca atttcagcta aagcctgatg cccggcacca aatactgtga tggcatttgt
    17101 tttttgcagc ttaacttttt tctgcatttt tttattcttt cacatctgtt atttgagtag
    17161 aattttattt cccagtttaa acgacttatg caaattctgc cagcatatga ataatgcatg
    17221 atctttgcgg tttttgattt tgaagtaaac actgtaaaaa gcacttaatt tgcatgaatt
    17281 gaggggacaa ttttacatct caaatcaacc tacaacaatt tacagtgcca aagttagcaa
    17341 aaattttctt atttgttgcg tttttaatgt tgacagcatt ttctgcagaa gcagcagaac
    17401 tttggcgcct gcgctatttt gtgccaactt ctgcagaaag tgaaatcaca tttggctcaa
    17461 gcaaggctgc tgaaaaactc aatacatccg gacacagtgg aaatttggta tttgcaaatg
    17521 gaatcggagt cggttacagc acgttacgtt caaacgtaag tctcgaagga gtttcctaca
    17581 tattcaggaa cgacagcctg gatttatcat acacaattgg gaactcgctc agttttactt
    17641 ttggagcagg tcgtttaatt aatggacggg gcgaactaag tctgaatgga gttagctatg
    17701 cgactgaaaa cagtactgga ggagcagtct tttttaattt aggtattcct tttattgggg
    17761 gagagttatt attgggttac cggcaaaata atatagaata taagaattat caaagccaaa
    17821 tttcaggaaa aacagtaaca cttgctgatc ctgtaaaatt actttcgagc caggtaagtg
    17881 tcggaatagg atttattttt tgaaaataat tctatatttt tattcttcaa cttattgaat
    17941 aacaacctgg cttaatgaca cagtatcatt atctgtgttt aatttatgcc gcttttatta
    18001 ttactttaca tgtccgaaat taaactgcga ttcatgaagc gcgctttaga actgggtgaa
    18061 caggttcgta ataaaactgg agataatcct tgggttggat gtgtgattgt aaggggtgag
    18121 aaagtgctgg gagaaggtca tacacatcca ccaggagaag cacatgctga agcagatgca
    18181 attcttaatg ctgaaaaaca gggccactct ctcaccggat caacgcttta ctgtaccttg
    18241 gaaccgtgtt ctttccacgg cagaactcca tcctgtgcag aaaccattgt tgaaaaacaa
    18301 atttcacacg ttgtaatcgc tatcagagat ccacatccaa aagttaatgc tgaaggtgta
    18361 cgaatgctca gggaaagcgg agtactggta actgagggaa tagaggagca ggatgcacgg
    18421 aagtctttga aagactggtt acttaaatac gaatgagaaa aaacattgaa tatccggagc
    18481 gccccattgc aggagtggga gttgttgttt tccgtaatga agaagtctta ctggttaaac
    18541 gtaaaaaagc tccatataaa ggacagtgga gcatccccgg aggtaaacag cggcttggtg
    18601 aaactgtaac tcaagcagct cgccgagaat taatggaaga gacaggagtt gaagttaatg
    18661 aattaacttt aattgacgtc atagatatta tagttcccga cgaagaaggg aaaatactct
    18721 accattacat cgtggcagat tatcgagctc attggctgtc aggagaatgc agtcccggag
    18781 atgatgcaca ggatgtacag tggttcaact taaacaaact cggctcgatt tctttgttag
    18841 ataaaacaag aaaaataatt cttaaagcag ccggtaaacc tgattcgacc tgagttgtct
    18901 gcaaagttaa atctttcctt gagctaattt actgcttaat accttagtta tccttttttc
    18961 gtttcctgaa tccctcatct tcccaggtca aaaagcgttt gccaatgaca tctcctgtgt
    19021 cttgaacaga gataaaagcc tctggatcag tatcaaacaa atattctttg agccgtccca
    19081 gttccagcat attaattact gtataaatca cctgcttttc ttcacctgca aatccaccgg
    19141 aggcatggaa ataagtaaat ccacgatcca gttttttcaa caccttttct gcaacttcat
    19201 caggcttgga ggaaatgatg aaaattgatt ttcgctgaga caatccaacc tgcccttttt
    19261 gcatactcca actgaaaaca tacatgaaga ctccagtaaa gagagccgtt tccaagtgat
    19321 aaaggtatgc gttactgaaa ataaccacaa tgtttaccag gttaaaaact gtaccaatag
    19381 gaattgcaaa gcgttttttt atcacagttc ccacgatatc cagaccacca accgagccac
    19441 ccaaacgcaa ataaaaaccg gtaccaaaac cagccaacac tcctccaaaa atggctgcca
    19501 acatgggatc atttattgaa atttcaactc catgtgttaa ttgaagactc aaagaataaa
    19561 tccccatacc aatcagtgaa gtgatgatga atttcagaga aaattctcga aagccaatta
    19621 taaatattgg tatattgaga agagcataga gtagactgaa agagatatat tcctttatag
    19681 aatcataaaa caataatgcc agtccaatca gtcctccggc aaaaaaattg tgaggcaaaa
    19741 gaattccgtt tacgacaaaa ctgaagataa agcaaccaag tgtcagcgtt agtagcatgt
    19801 accaaaactt caaagtacgt aatgcatttg taaattcttg aaaatcttcc ttaagataat
    19861 tcatttgatt taaagtagtt ttgttgtatt tgcagaataa ggacaaaaaa aaacgggatg
    19921 accgctcgtg caaccatccc gtcttaatcg cttttttcct aaaatgaatt atgaagcagt
    19981 ttgaacacta accgcacaag ggcctttttg cccttctcca atttcgaatt ccacattttc
    20041 tccttctctg agatatcccc cgcggacctc agaatgatga acgaacaagt cttttccgga
    20101 ctcctgctcg atgaacccgt atcctttacg ttcgttgaac cattttactt tgcctgttgc
    20161 cattttgacc ttatatttta attaaaaata ctcttctaaa tgaagagaag aaaccataat
    20221 tatgacaggg aaaataataa atgcaagaaa aaaatgcatt tcactttcca ctttgtcaaa
    20281 attatttatt tcgcaggcac agagaccagc tcagtttcct tctcaagtgt ttctagcaga
    20341 ctggcgtaaa ctccgctaag ttgcattagc tcagcatgtg atccacgttc aactatttta
    20401 ccggcatcaa taacaagtat caaatcagaa tttcgaattg tactcagtct atgtgcaatg
    20461 gcaatcaccg ttttatcatg aaaaatgcgt tcaatggctt tctgaataag attttctgta
    20521 acagaatcaa cggaactggt ggcctcgtcc aatactatta aatcgctttc acctgccaat
    20581 gcacgggcaa aaacgatcaa gcgtccttct ccctcagaaa gattttttcc accttcgaga
    20641 attttgaaat catatttctc aggcaggagt tcaataaatg tatgagcata gacatatttt
    20701 gcagcttctt tgactttttc aggagaaact tccggacggt tcagagcaat attgaagccg
    20761 atgctttcct caaacaaatg cgaatcctgc tgcatcattg ctatcatcaa ctgcaaatga
    20821 tcccgtgata tttcgtaaag ctccacacca tttattttga tagaaccctg ataacgtgta
    20881 taagttctgg taagaatacg gacaattgta gattttcctg aaccagttgc tccaacgata
    20941 gccaggcgat cgcctttacg gactataaac gagactccct gcaggaccca tggtccatcc
    21001 tctgtgtagc ggaagtgaac attttcaaaa cgtaattctt caaattcctg aaaacgtttc
    21061 ataatttctt ctggagtata ttttctggaa ttggtcgcat aggaaggcat gtctgccgta
    21121 tcttgatctt ccggaatttc acgaaatagt ttgtcaatat tttccagtgc agaaagcgag
    21181 cgctggaaaa cggaaatttg ttgagtaaat tctcgaattg gaacaaaaat gcggttcaac
    21241 gtatttatga atccaaccaa aactccaatt gagacaagtc ctgtaagaat ttgctgggct
    21301 ccataccaaa gcatcaaggc cagtgcaatt gaggtaatac cttcaatcac agaaaaaaga
    21361 gctgcatcaa aaaaatttga gtgggtctgt gccttaaaaa aatgcctggt ttttgcttca
    21421 aattgttttc gaactttggc ttctgctgca tacaactgga ttgtttttac accattcaaa
    21481 cattcctgca ggtaaccagt agagtccgca agcgcctccc gagtcagatt gtaataatgc
    21541 cgcaatctcg acctcagaaa attgctgaca aaatatattg gaggtaaaat cagcagcact
    21601 actagcgtga gcttccaact caggattatc aacaatatta gcaacgcaat tgtcttaaga
    21661 aagtcggtaa aaatagaaag tacaccaatg gctagagaat cattgagtgc ttccatgtca
    21721 cttgtgagac gtgtcaaaat cacaccaatt ggacgctggt caaagaaaga gcggggcata
    21781 gaaaggatgt gcgcatagag atcactgcgc atcgcactaa tagctaactg gccggttttt
    21841 tggaggtaaa atgtataaat tgaatctgaa aaataaccga cagcaactgc tccagccatg
    21901 agaaccacca tccgaaacaa tccgtccaga tcaccgggaa ttacatgttc atcaataatt
    21961 tgaattaata gccatggcag gagcaaagtt gccgcaattg agaacggaac tgtagcaact
    22021 cccagtacca cgcttttcca gtacggacgc acgtaaacga gaaaccgctg aaataattcc
    22081 cagtcgttga cttctttatt caatatacga ggtttcaggt ttcaggtttt tatttacgat
    22141 accgatgagt ttaaatagtt tctgaacgac aatgcaacca gtcaggtttt tcaagtttat
    22201 tacagaatag aaatggacaa agatttatag ccgttgccga atggaagcca actggacaca
    22261 gattacaaaa atctgcattg cctgatccaa atcttcaaca cttggaaacg tgggggccag
    22321 tcttatagtt ttatcattgg gatcatgaga ataggggtat gttgctccag ccggagtcag
    22381 tttaactcca gcctcaccgg caagacggat tatttcctgc gccagtccgg gcaatgcgtc
    22441 aaacgagaca aaatatccgc ctttcggatt agaccaggtt cccacacctt ttccagccaa
    22501 ctcgctttca agatgttttt gcaccattgc aaacttgggc cgcagaattt cagcatgttt
    22561 tctcatatgt tccagtacac ctgctaaatt ttttagaaag agaacttgcc tctgctgatt
    22621 gagtttgttt ggaccaatgc tcatgacggc aaggcgttta cgaaaatgtt ccaggttttt
    22681 ctcagatgca cccaaaaatg aaatcccgcc tcctgcataa gttatctttg aagtagatgc
    22741 agttaaaatt aaattgtcaa cagttccata ttttcggcag aaatccatca catttgccaa
    22801 ctgctgcgga ttttcaaaga ggtcatgtac tccgtatgca ttgtcccaca tcacgcgaaa
    22861 atttgagcct gaaattttac caagtgcagc gattctttcc accacttctt cagaatatac
    22921 atttcctgtg ggattggaat atttaggaac gcaccacatc cctttgatca tgtgatcatt
    22981 gcgcaccaaa tcttctactg cgtccatatc aggtccgtca tccagcatgt ctacactaat
    23041 catccgcagt cccagttcct cacagatgga aaaatgacga tcgtatcctg gaacaactgc
    23101 cagatatttt acttcacctt ctctggccca ggctgtatta ggtccttgag agccatgata
    23161 aaaagcatga agcatgtaaa gatacatcag tgacaaactg ctgtgatccc cacaaatgac
    23221 ttcggtttcc ggaaggtcaa gcatttcgcc acccagttta cgtgctgcag gaatcccatc
    23281 gagtccccca taattcctga gatcagttcc atcctccaaa atcagtttgt ctttgagcaa
    23341 gccttccatt tcatcagcaa gctccagctg tgcagttcct ggttttcccc tggtaaggtc
    23401 cagatttaaa gcggcatctt tatatttctg atacttttct gcaagttttt gttccaaagt
    23461 ttgcagttct tctttggttg cattttcaag attcatagtt tttttgtttt ggtttaagtt
    23521 tacgttttta ttatcagttc cgatccataa agtgtcaatc ctgaatgtct catcatttcc
    23581 tgttttactt ttctttaatt tctgaaaatt tttatttcca ttcaataatt tttttacaca
    23641 acggtccccc gtcaccaaga aaatcatgaa tgatattgaa atggtttttt ccgggcatag
    23701 atagatattc tgcaggaact ccgtgttttt gcagaaaaat agtataattt ttcgattgcc
    23761 gctgaaattc atgcgattca tctgcgccca ctgcgaccat aacatgtgga gtgtacacag
    23821 gtttaaggaa tacaggactg ttgcggagaa cttgttctga agtcagcagc aattttggct
    23881 ggagccagga aaacggaaat ggttttaggt caaaaagtcc actgacaggc aaaaatccct
    23941 tgatcaaatt tggagttaaa ccatagtttt tttcccaatc tgtgctcagt aacattcctg
    24001 tcaaatgacc accggcagaa tgtccggaaa ccgagatccg ctccggattt ccgccaaagt
    24061 cctctgcatt gcgataagtc caggctgccg cggcacgcga ttgacgtaca atctcatcga
    24121 tgttaactga aggacaaaga gcatagttaa ccagcaccgc cgtaattccg tttcgtacca
    24181 acccttctgc cacaaacgca aaatccctgc tcgtcaacga atgccagtaa cctccatgaa
    24241 aaaaaatgtg aataggacta cagagttttt cagcaggaaa aatgtcaagg gtttccgacg
    24301 gtgttggacc ataagccact gcggaacggt ttgaatattc tctgaggact ctttggcttt
    24361 cggtaaaata gctttgaatc aactcatctg tattctctac aataaagcgc ggattgtatt
    24421 cctggtctat ttcttcttgt gttgaaaagg tgtggtatag cattttaaag tcagtctttc
    24481 tgcgattttt tctcagtcca aatcctcaga tcccatttat aaaggttttt ctacatcacg
    24541 ttttatatat aacggttgaa ctggaagatc cggatcaaat gcagatgaag ctaatttata
    24601 tgcacccagg cgatctaaaa gctgagcatc cggaactgga taatccaagg gcagcagaac
    24661 cagttgttca aacagtggct catgttttcg ccgctcaggg ttgacgcgat aatggacaac
    24721 cggaacaccg cctgagattt caatgaattt ttctagactg gtgagttgaa ttgcagtctg
    24781 tgctaccgga tgatcatcta ctatgcgaaa aactgagtga aagatttctc tacgggtaca
    24841 gttaagcaat acaggaaaat catttttcag gtgcctgatt tgtcctgcaa gtaattcaag
    24901 tgaattgact ggaattaccg gtttatcgtg aacctgggcg aaagttttgg ccacgctcat
    24961 tccaattctg actcctgtgt aagagccggg accgcagttt acaacatagg catcaacttc
    25021 cgctaagctt aactctgcgc ttttcagcaa atcatcaata acttcaaaaa taattgttgc
    25081 attcctccga tgacacagag aatagtaatt tgccaaaggc actccatttc gggtcaaaca
    25141 caaacccaaa tgttcaaatg aagtatccac tgagagtcta atcatgtact atcctgattc
    25201 aaaggggctg ttgttttttg taaagattaa atcgattgtt acaaagtttt taaaagtgtg
    25261 tcaaaatcat caaattcact tttaatgatt aattgtcttt gatattctga aacggttttg
    25321 aaatacaagt ttagtacggg ttcatccgtc aaatgatctg taataaattc cggccactca
    25381 accaaagtaa ttccatcatc ggcaaacaga tcttctctat caaaatcatc gagttgctcc
    25441 ggtgcagtca agcggaagag gtcaacatga agaattgtcc atttgcctga ataaatgttg
    25501 accatagtat aggttggaga aataactgtt tcatgaggga tgcccagacc ggcacaaaga
    25561 ctttttgcaa aagtagtttt tccggcaccc atgtttcccc aaaacaagag aacagcacct
    25621 ggaaacaaat gtgctgcagt ttttctggca acaacatcag ttatttccag tgaatttagc
    25681 tcaagtatta gtttactcat caaactgtca tgaatccaag agtgctcatt agttgtcata
    25741 atttaggcta tgctgcacct tccggaaccg gaatatctac tgtagaaaag tgggacatac
    25801 ggaagctgaa tttggaatta catgctggag agaaagcttg tttccatttt caaaatgagg
    25861 agcagaaaca agtattgtgg cgtatgtttc agcaaaagct caagcccaaa actggttctt
    25921 tacagatttc agccaatact caccttcaca ctgacgacag cttatgggaa ggtacagaca
    25981 agaaagcgtc actactggaa aatatggaat ctaaactttt ttcgactcgt ccgtggtttg
    26041 gaggacaaag aaaaaatatg gatattttga aggaccgttt aggattgacc ggacgaatta
    26101 ttaatctccc cgttaatgaa cttatgcctg agtatgcagc acgattttgg gctttgatgc
    26161 tggttgcggc aaacacaaaa gtagttttga taaaccgctt actttcacaa ttggacgaaa
    26221 taagtttgcc ttttattcag gaatggcagg aaagtttttc tggaattaca gtaatattcg
    26281 gagaacatcc tgaatatttc aaggcagtta attcaagaaa agtagaacaa caggacacat
    26341 tgaaatcttt tttcagcact aacatcagtt tttctgcaga cggacttgca aaacattttt
    26401 gatggactgt taactcaaaa gcagtaagcg tcagttagct acttcaaggt taaggtccga
    26461 atatttataa aatttcttta gctcaaaatt ctcgaaatac taagaaatgc aatttagtaa
    26521 aagctttatc cgccaagttg cagatgcaac ggaccttgtt gacttgattt ctgatcatgg
    26581 cgtcacgctt aaaagagcag gttccagcta caagggactt tgtcctttcc attcagaaaa
    26641 aacgccctca ttcaatgtaa atccggagcg tggttttttt cattgttttg gctgcagcgt
    26701 cagtggagat ggtataaagt ttctgatgca atatgatcgt ctttcttttt cggaagcagt
    26761 agaagagctt gctaaacgcg ccggtatccc tctggaaatt cagtccggag gaccacggag
    26821 ttccaatccg gaagaagacc gcggattaca ttgtctcaag gaggcagcgg ctttttacag
    26881 tgaaaaactt tctatcccgg aaggagaaaa tgctgtcaaa tatctacagc agcgggaggt
    26941 tccggaaagc atgtggaaac aatttcaaat cggaatgtct ccggacgaat ggcagggtac
    27001 tttgaactat ctacagaaaa taaatatttc tctggcagaa atgtccagaa cgggtttgat
    27061 caaggtcagt gaaaaatcag caagacacta tgatactttc cggggacgct tgatgtttcc
    27121 tgttaaggat gtccgcggac gctgcatcgg atttggggca cgtgcgatga aaccggaaga
    27181 taagcctaaa tacctcaaca gtcctgaaac ttcatactac cgtaaaagcc aggtacttta
    27241 cggcctacat gaaggactta cctccattcg taaaaaacgt cgactgattt ttgttgaggg
    27301 ctaccttgat gtcatccgcc ttcacgaaaa cggttttact gaagcagtag ccccatgtgg
    27361 tacagccctg actcaggaac attttaaaat agccctgcgc tacgcagata cagtaatttt
    27421 gctttttgac ggtgattctg caggaaataa tgccgcactc aggcatgctc atcttttgct
    27481 tcctgtcgcc cttgagtcct acatccttac tcttcctgaa ggagaagatc ctgacacatt
    27541 tttattgaat aacggaaaag aaaattttga tgcattcctg aatcagaaag ttccggctct
    27601 ggattacctg gttcaacaga ccataaaaaa atatccagac agtatccagg gcagaatgca
    27661 gggtctggat gaactgttgc cggcattgta cgaaatccag gatcctaaac gtcgacaact
    27721 cactctgatg acaattggtg aaagaatgaa gattcctccg gaattattgt cgaaagagtt
    27781 aaaaagaaaa gaaaacaaaa gcttgcataa aaataaaaat aatgatatag tcagttgttt
    27841 atctgagaat aataaagatt cccaagatga acagtggctg ttacagtcat tgttgcgcaa
    27901 aggcaaatta tggccccgtg tccgggagca tctaaaaccg gaagaattcc agacaccaaa
    27961 tttacgccgg ctttacgaaa aattattgca gctacctgat gcagtatttc aagctttcga
    28021 tccttttact ctggagaaat ctgatcctga cctgtttcaa tcattgatgt tgctgctttc
    28081 tgaggaaatt ccaagtcacg actttgggct ttcactcttg cgcatgaaag aacgaaactt
    28141 ggagattgaa tatcaggagt ggctacttca cagtaaaagt aacgaggagc gggcacaggc
    28201 tgctctaaaa agaagactcg aagaaaagaa tttgaaagaa ataaagcaaa ttatcgacaa
    28261 tatattgact caataatctc tgtatacagc acatgaacac agcaaataaa agtgataacg
    28321 aattttcatc agtagagaaa aaacttatca aagagctggt ttcggatgga cgtgaaaaag
    28381 ggtttgtcac aattgacgaa atcaatgaaa gtcttggcga tgaaactcca tctccagaac
    28441 aactggaaaa aatatttaat atatttgctg aaatggacat tgaagtaatt gattcggaga
    28501 aaaaaatgga tattgtaagt gatcaaacag attctgctac tgaactggat aatgagactg
    28561 gtgaactcaa ccttgaccct ggaatagata attctacaga cgatcctgtt cgcatgtatt
    28621 tacgtgagat gggaagtatc caattgttgt cacgtgacgg agaagttgaa gttgccaaaa
    28681 gaatagaaaa aggaaacgca gacttaatag atatattctc tgattcaaaa ttgattttgc
    28741 tttatctgca gaaccttacc aagcacctca aaaacaagaa aataaaggca cggcatgtta
    28801 tttcaggatt ggatgaagat gacaatgtga ttgaagacga agacaaggct accgtcaaac
    28861 taattgctca attcgatgta ctttggaagc accatcgttc tgtgaataaa ttccaaaaac
    28921 atcaggccaa acgaaagctc caacctgctg aaaagaaacg tctggaagaa agcgaatcat
    28981 tattcaaaga tgcaattcgt aacattagtt ttaactcacg ccaaattgaa ttaatgtgcg
    29041 atgagatatt aaatcacaag gaaaagatca acctgacttt ccgcaagatg aactccaata
    29101 taaaaagttt agacttgccc gttgaaaaaa tggataaatt gtttgaacaa ttgagttctg
    29161 caaccagtaa aaaagctaaa gagaaaaccg caaaagacat ttctgctaaa acaggacatc
    29221 cgttcagtat agcaaaccgt tactttgaaa aaatagaact gaaacggaag cgtatcagca
    29281 caatgatcga actgacaacc ctctccttgg atgattttaa ccttgaagta aaaagtctta
    29341 aacaagtaga gagaaaggtt aagtctgcaa aaagccagct agtggaggca aatttacggt
    29401 tggtgatcag cattgcaaaa aaatacacaa accgcggact gcaatttttg gatcttattc
    29461 aggagggcaa tattggttta atgaaagctg tagacaagtt tgagtatcgt cgaggataca
    29521 agttttcaac ttatgcaacc tggtggattc gtcaatcaat cacacgtgcc attgcagatc
    29581 aggcacggac aatccggatt ccagttcaca tgattgaaac aatcaataaa ctctcgagga
    29641 tatcacgagc caaggtacag gaacttggac gtgaacctac tgcagatgag ttatctgaag
    29701 atcttggaat gcctttgtac aaagttcgta aagtactaaa aattgccaag gaaccaatct
    29761 cacttgatac accaattggt gatgatgagg attcgcaact caaagatttt attgcagatg
    29821 aacacataag ggatccaggt gaagctactg tttcttcaaa catgggtgaa agaacacgtg
    29881 aagcattaaa aactctcact aagcgtgaag agaatgtgtt acgtctacgt tttggcattg
    29941 attcaatcaa agatcacacc ctggaagaag ttggtcagga ttttgatgta accagggaac
    30001 gtattcgtca gattgaagca aaagcactgc gaaaattgcg tcatccaacc cgttcaaaat
    30061 tactcaaatc attttacgaa taatgaataa acggggccct tggaattcag gtatcatttc
    30121 taaaatgctg gaaagcggaa attttcatca actgaaatga ctgtttcatg ctctcaatca
    30181 ggtccatgat attcttttca ggaatgagtt ccaggagtat ttagactttt caagattaca
    30241 tcataatcag gtttaagctt caaaactgtg tagaaaatac taatccttgt tgcttgaacc
    30301 ttgaaccata ccacttgata ctgatctttc ctgtatgagg ttgttccgga attttctaat
    30361 tctgctgatc atcagctgtt cgactgctgc aggcattccg gcgcaaactt ccaagacttc
    30421 cgatccagtc ataattcaaa atgtagagct actgcgtcac ggcaggcaaa tcagcgtgcg
    30481 tctgctgaca gacaatccac cagtttacgt aatcacagaa aaccttgctt cccggacact
    30541 ggtgattaag ttcaacaatg cccgtattgg tttttcagac ggccggaatg agcgtctttt
    30601 caatgatccg cagctcgcag gaatacgttt ttcagcaatt gatggagaca gctgggcaca
    30661 gttcaaatta ctgaaaaaag atttaagtta ttctgtcagt tccctcacgg aacaaaatgg
    30721 aatccaaatt gatttccgtc ccgcactcca aattgaaccc ctgcctgatc cgccggaaga
    30781 tgctgcgtat caactggttt ctataaaatt tgatgccggc aatactgatt ttacacgttt
    30841 gactttcggt tttacaaaac ctgcaaaatt ttctgcaact ggttctgttc cggaagacag
    30901 gcagccgccc cggatttttt tacttgaaaa cagccttcaa actcaactta ctattcgcct
    30961 gcctaacact ttaccggaaa aacagctgtt aaaccttgat tacaaggatg cccgtttgga
    31021 gcttcatgca ctcacttcag aagtaaacca aacctttatt gaattgaaac tcaatgcaac
    31081 agacgtgagt gtggaacggc attttgtttc caggcccgtc cagtggatac ttgacattca
    31141 cggtactccg ttaaagggca aaattgcagc agatcttacg gaagggaaaa agttgagtcc
    31201 ggaagatgct gctgcatttg aaaaagagga aaaagagcgc agaatcaggg cttctaaaat
    31261 caatcctatt tttcagcgcg gtgaaaatgc tttcagaaaa aatttgtacg atgaagctgt
    31321 gtcccattac aaagaagcct atgcgctcgg gaaggaacac acaggagatg aatttgaaga
    31381 tccacttcac cctttgtctg cacgtgctct cttccggatt ggagacacaa tctacaccat
    31441 gctggagcgc aggattggtg ataattatca ccaggcaatt gaggcttaca aaacagcaat
    31501 ccgtattact caggatgcgg aaaaaattgc caaagaaaat ggagaagaat ttgaaacagc
    31561 ttatctttta ccacatgcca atttccgaat aggacgctcc tatcaaaaaa tgaaatttca
    31621 gcatgaggca acagtttatt acaaaatact gcaggagcgt tttcaaaatt caattgaggc
    31681 aatggaagcc agtttttgga agggaatgag ccgtattgag caacggcaat gggaaatggc
    31741 catcaacgaa tttaaagaat acctgcgtac ttcacctgaa ccaaaatttt ttgctgtcac
    31801 ccattataaa atggcacagg cctattacca gcttaagcgt tacataactg caaaggagtt
    31861 ttttgatata gcacggaatg cagaagagga ctatgccaaa gacgatccaa cgcttttatt
    31921 tcatatgggt gagacatatt atgaaaatgc cgattacgtt actgcacgtg aaattttcag
    31981 attactcctg gaaaaatatc ccaaagcaga tttttccaag ttggttgcac tccgattagg
    32041 cgattttctg cgggatgaag gcaaagaaaa tgatgccatt ctagcctaca aaaacgcaat
    32101 cagcagttac accagagaga ttgcgctact gggcaaattg cgaattgcca acattcaggc
    32161 aaaacgccct tattctgatg aacatcttga ggcaatcacg gtttatgatg aaatacacag
    32221 actctatccg gagacaccac aggcgaaaga agcactgctc cggaaggggt tgacacttac
    32281 tctttacgga tattacgcac gtgcaattaa aactttggaa agtttcatgg agaaatatcc
    32341 tcaaaacgtt tatgtgcgca gaaatgtaat acaggaaaac attgacgaga atctaaaagg
    32401 attgattgac cagtattttc gccaggatga cacacttgct ctcgtaagcg cttatagtga
    32461 ttataaagct aaatatttat tcaatttccg ctttgacaca accctttttc aaactgcggt
    32521 agctcaccgt aaactgggct tttacgagga gacactggat attttgcgtt ttcttgaaac
    32581 acgctcggga ggaacaattg gcgaactggt acagttggaa aaagccctga cactgctgga
    32641 aaaagaagat ctgactgaag cccgtaatat cacagcagga tttctacaaa aataccctgc
    32701 aagtccttat gatgcagaag cacggcaatt gctggcaaaa atttaccgca ggaaaaaatc
    32761 atatccaaat gctctgcttg tttacaatca aactctgcag aaatatgatc aaaacaagga
    32821 tccgctttca gcagaaattg ttccggaact ctactatgat ttgggagaac tccacgaaga
    32881 aacaggaaat tttgtagatg ctggtgaggc ttttcaagaa gcggtttcaa gttacaatca
    32941 tccgcttgat catccggata caccagaata tattatcaac agccattttc tggcggcaga
    33001 tatgtacaac aaagctcaaa acgatacaat tgcgcttctg agctatcaac aggcaatttc
    33061 gctctatgct gatagcgaaa acaaggaaat aaacgagcgt gtgttttggg cccgttatca
    33121 aatcggaagt atttatgctc gtcaggaaaa caatgagcag gcattaaaaa tattcaaaga
    33181 actggtggat cacaaagatg gtg
//
LOCUS       DTVD01000060            2716 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15442_length_2716_cov_441.736565, whole genome
            shotgun sequence.
ACCESSION   DTVD01000060 DTVD01000000
VERSION     DTVD01000060.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15442_length_2716_cov_441.736565"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="EYO68_02285"
     CDS             complement(<1..740)
                     /locus_tag="EYO68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine 3-dehydrogenase"
                     /protein_id="HIC06662.1"
                     /translation="MSKLIKKTALVGGGVIGGGWAARLVLNGVDVKVFDPSDTAERTL
                     KEMLANAELAYSKLTMAPLVKQGNIEFVDSIEKAVKNVQFIQESVPENQDFKRSIINE
                     IAQFAPESALICSSTSGIKPTSLQTKMRHPERFMVGHPFNPVYLLPLVEICGGEKTSD
                     AAKQSAAEFYRNIGMKPLILRKEIDAFIADRLQEAVWREGLWLIRDDVATTEELDDAI
                     RYGFGLRWAQMGLFETYRVAGGEAGMRH"
     gene            complement(741..1652)
                     /locus_tag="EYO68_02290"
     CDS             complement(741..1652)
                     /locus_tag="EYO68_02290"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06663.1"
                     /translation="MLIRAYTSIIAACLLWSLNPAANKLALEEIAVPQLVFMRAVFAA
                     FILLLVSFFMGHRFRPKEIGWRPFLLGVLDPGLTSLLFVTSLTVLSASNTVLIMALMP
                     FSQSVLGRVVFKEELRSSVLLGGTLAFSGIVIYFSGEAFNGADTVWGNSLLLSVFCLM
                     AVSQLMTRKIMTTEIPAIQVTSSQMISASVVLFIYLILFGDTELPLQASSGTMMTLLF
                     LVFSMAIPFFLYNQAMRHLPIGMASLLLVMIIPFGFLFAAIFLGEEITLIKAIGAILV
                     MTGVAFPHFPQVRRKFIRTNENPFDRE"
     gene            1818..>2716
                     /gene="gcvA"
                     /locus_tag="EYO68_02295"
     CDS             1818..>2716
                     /gene="gcvA"
                     /locus_tag="EYO68_02295"
                     /inference="COORDINATES: protein motif:HMM:PRK11139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HIC06664.1"
                     /translation="MNNFTGLPSLNYLHTFESVARHLSFTNAAKELCVTQAAVSHQIR
                     ALEEHLGVRLFHREKRKVLLSEEGLKLLPSVVSGLQGIADSLESIHNYDSEDTLVVGV
                     GSSFSANWLVHRLGTFYQQYPEVNLHLKITNNDPDFAADETDMAVVWGKGDWEGLMCE
                     QLMVVEFTPVCSPELLKNSQPLRTPEDLIHYPLLDDSDYESWLEWLEEAGIPERKYKR
                     RMVIRDSNVLINSALDGHGIALCAVGIVQEYLDSGRLVRPFDLSITGGGFHFLVYPEK
                     ALRKPLVRLFKDWLISEVTKTEF"
BASE COUNT          856 a          587 c          560 g          713 t
ORIGIN      
        1 aaatgacgca ttccggcttc acctccagca acacgatatg tttcaaacaa ccccatttgc
       61 gcccagcgca gaccaaaacc ataacgaatc gcatcatcca gttcttcagt agtcgccaca
      121 tcatcacgaa tcaaccatag tccctcacgc catactgctt cctgcaaacg gtcggcaatg
      181 aaggcgtcaa tttctttgcg caaaatcaaa ggtttcatgc caatgtttcg ataaaattct
      241 gcggcagatt gtttggctgc atcggaagtt ttttcgcccc cgcagatttc aacaagcggc
      301 aaaagataaa caggattgaa gggatggccg accatgaaac gctctggatg cctcattttg
      361 gtttgcaggg aggtcggttt tattccggaa gtggaggaac agatcagtgc agattcagga
      421 gcaaattgag caatttcgtt tataatcgaa cgtttgaaat cctgattttc cggaacactt
      481 tcctgaataa actgaacgtt cttgaccgct ttttcgatgg aatccacaaa ttcaatattg
      541 ccctgcttca ccaaaggagc catggttaat ttggaatagg ccagttccgc atttgcgagc
      601 atttccttta gagttcgttc tgcagtgtca gagggatcaa agactttcac atcaactccg
      661 ttcaatacca gtctcgcggc ccatccaccg ccgatgacac caccaccgac aagtgccgtt
      721 tttttaatta acttactcat tcattctctg tcaaaaggat tttcattggt tctaataaac
      781 tttcttcgta cttgcggaaa gtgcggaaat gcaactccag tcattaccaa aatggccccg
      841 attgctttga tcaaagtaat ttcttctcca agaaaaattg ccgcaaataa aaatccaaaa
      901 gggatgatca taacgagtaa taaactagcc attcctatcg gaagatggcg catggcttga
      961 ttatagagaa agaaaggaat ggccatcgaa aaaaccaaaa acaacaaagt catcattgtt
     1021 ccggaagatg cttgaagcgg aagttcagtg tctccaaaca atattagata gatgaacaaa
     1081 acaaccgacg cagaaatcat ctgactggaa gtgacttgaa tcgccggtat ttcagttgtc
     1141 attattttcc ttgtcattaa ttgacttaca gccatcaaac aaaaaactga cagcaaaagt
     1201 gaattgcccc aaacagtatc tgctccatta aacgcttccc cggaaaaata tatgacaatt
     1261 ccagaaaaag caagagtgcc acctaacaag actgaagacc ggagttcttc cttaaaaaca
     1321 acccgcccaa gcactgactg agagaagggc atcaatgcca taatgagtac cgtgtttgaa
     1381 gcactcaata ctgttagtga agtcacaaat agcagagaag tcaagccggg atcaagcaca
     1441 cccagcaaaa atggtctcca gccaatttct ttaggacgga aacggtgccc cataaaaaaa
     1501 gacaccagca acaaaataaa tgccgcaaaa accgctctca taaaaacaag ttgcggaaca
     1561 gcaatttcct caagtgccaa tttgttggcg gcaggattca gcgaccataa aagacaggca
     1621 gcaataatag aggtataagc acgaattaac atcacaaaac tccaaagata gattagttat
     1681 gtttattaat aaacaaatta aaataatggt aaataaatta aagtaaaatt acacttgcat
     1741 ctattttgta tttgttttac aagagataag tattttttac aattgattat ttttcatcat
     1801 taagattagt ttagattatg aataatttta caggactgcc ctcactgaat tatctacaca
     1861 catttgaatc cgtcgccaga catttgagtt tcaccaatgc agccaaagag ctttgtgtca
     1921 cacaagcagc ggtgagccac caaatcaggg ctttggaaga gcatctcggc gtcagattgt
     1981 ttcatcggga aaaacgcaaa gtacttcttt ctgaggaagg actaaaatta ttaccgtctg
     2041 tagtcagtgg attacagggc attgcagaca gtttggaaag cattcacaat tatgattccg
     2101 aggacacctt agtggtcggc gtgggctcgt ctttttccgc aaactggctg gtgcatcgac
     2161 tcggaacatt ttatcaacaa tatccggaag tcaatctcca tttgaaaatt acaaacaatg
     2221 atcccgattt tgctgctgat gaaacggaca tggcagtagt gtggggcaaa ggagattggg
     2281 aggggcttat gtgtgaacag ttgatggttg tcgaattcac acctgtctgc agtccggaac
     2341 tgctgaaaaa ttcccaaccg ctgagaactc cggaggatct cattcactac cctttgctcg
     2401 atgattcaga ttatgaaagt tggctggaat ggctggaaga ggctggtatt cctgaacgaa
     2461 aatataaacg acggatggtt atcagggatt ccaatgtact tatcaactct gcactggatg
     2521 gacacgggat tgcgctctgt gcggtaggaa ttgtacagga atatttggat tccggccggc
     2581 ttgtgcgtcc ctttgatctt tcaataacag gaggtggttt tcactttctt gtctatccag
     2641 aaaaagcttt gagaaaacca ttggtgcgtt tattcaaaga ctggctgatc agtgaggtca
     2701 caaaaactga atttca
//
LOCUS       DTVD01000061           10712 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1548_length_10712_cov_322.858966, whole genome
            shotgun sequence.
ACCESSION   DTVD01000061 DTVD01000000
VERSION     DTVD01000061.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_10712_cov_322.858966"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            162..1697
                     /locus_tag="EYO68_02300"
     CDS             162..1697
                     /locus_tag="EYO68_02300"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIC06665.1"
                     /translation="MLDHWVWSRYLSFSYYEHPPMIAWLIRGITFIGGNNETALELGS
                     QLVTLSILTLVYAGTFRLYGIKPALVTLLILCSMPYFTLGSIFLHITQPFLIFWILAL
                     FLLIRFHQQPANNWLLWIGVAAGFGALSKYIMLLFYAGLFLHLLLYHKTRKDVLNPWF
                     YFAGMLSLLIFIPVIIWNAQHDWVSFRWQLEKGTSGADFGENTLAFTVGHLLLFSPLW
                     ALMGGLGIWWLRDRLVEARSPESVITVISIFPLLFFTVMSLKGTISDPHWANLAYLGI
                     AIMLGKELLRRFQKKTLYVLLASGLLINVALTGTVVTHALNPLFDWMPYELKNYDYLK
                     NNNVPQATLEKLRNNDERLYSTGQYRLHLKQILSPAEIEEYGGLIQKTAMDISSDRLT
                     RVLEWDKTGEQLQQLLVQNGIPQIAFIVSREYQLSGALSFYLPHQPWPHSIEKPERNL
                     WSPLDEVKMGPSIFVCELQECQGGVVDFYERFEMPLRYLGEIETRRYDRMVRNLQVYE
                     LIP"
     assembly_gap    1786..1885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1886..3048)
                     /locus_tag="EYO68_02305"
     CDS             complement(<1886..3048)
                     /locus_tag="EYO68_02305"
                     /inference="COORDINATES: protein motif:HMM:PF16916.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIC06666.1"
                     /translation="MQTTDLTEKDALKVTWLGLLANLLLALAKGFIGFIANSSALIAD
                     AGHSVSDLLSDGITLWAVRMSGIPKDKNHPYGHGKFETVGTFIVALLLLFTGIGVAWH
                     VFNKMDSPEVPGTIALWMAGIAIAVKEVLYHATRMVARRTGSRILLANAWHHRSDAIS
                     SVAALVGIGGAQWGLPLMDPIAGVLVAGLIIKTGINIGYESIRELTDETAEEEVISEL
                     GQILSGIEGVDHYHEMRARRMGPHLLVDLHIEVDSMMSISAAHQVAERVRLGILEKLP
                     AVNEVLVHVDAEDDFFEAEGTEDPQKIVLMRPQSEIENDVKKALSVIPEIEGITHIYC
                     HYLNQELTVQVNILLDVEMRIRDAQKIASKARMKIEQIKDIDAADLHLELDDA"
     gene            complement(3052..3825)
                     /gene="gloB"
                     /locus_tag="EYO68_02310"
     CDS             complement(3052..3825)
                     /gene="gloB"
                     /locus_tag="EYO68_02310"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIC06667.1"
                     /translation="MEIVTIPCLEDNFAYLLICPKTQQAAVVDPSEAAPVQKAIEQQG
                     VELTTILNTHHHWDHVGGNKELKAKYPELIIYGHESDRGRIPEQTVFLKSGDEVRFGE
                     QTGTFLHNPGHTSGAITFVFGKTAFTGDTLFAAGCGRLFEGTPEQMHDSLNIQIGGLP
                     DDTELYFGHEYTAANLRFALSVETGNTEINKKLAAVTALRSSGKFSTPTTLAEERRTN
                     PFMRCSSAEICETVTSREPGHNLSEKEVFRTLRELKNNF"
     gene            complement(3835..5310)
                     /locus_tag="EYO68_02315"
     CDS             complement(3835..5310)
                     /locus_tag="EYO68_02315"
                     /inference="COORDINATES: protein motif:HMM:PRK00286.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIC06668.1"
                     /translation="MQIPFEVDSPQKDENSKKEAPISNPSIPHISQRTQDPQNPASKQ
                     EKKPLTVSQLTRQISLLLEGKFRSLTVEGELSNVKRAASGHWYFSLKDDQSQIRGVMF
                     RNAAAGLRFEPEDGLEVVVRGHLTVYQQRGEYQVMVSSMEPKGLGALQLAFEQLKAKL
                     DAEGLFAAEHKQSIPFLPRRIGIVTSPTGAVIHDMLTVLERRFDGIPVLFFPVQVQGE
                     MAAPSLVEAIQHLNTLRNSQQIDVLIVGRGGGSIEDLWAFNDEKLARAIFSSDIPVIS
                     AVGHETDFTIADFVSDLRAPTPSAAVELAVPKKEDLEHTVSQKQERLQRTMQQQLDRL
                     REYVEAVRLRLASPDAMLQQHAQRVDDLDSLLRERTKRQLENLRNRISGIAEKLFLLS
                     PGRELETQQHSLNLLQDRLTRAIGLLQIKLSDSVQQQMDLLDSLSPLSVLHRGYSVTL
                     DNKGKSLRSVKEVHSGDKLQVRMEDGTLQTKVVSVKPKHPN"
     gene            complement(5333..6175)
                     /locus_tag="EYO68_02320"
     CDS             complement(5333..6175)
                     /locus_tag="EYO68_02320"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIC06669.1"
                     /translation="MTEILSGRTRIYGIIGCPVEHSFSPLMQNAAFDALKIDARYLAF
                     SVKPEQIPQAIAGIRALNIHGVNVTVPHKSSVISCLDEISPLTQLIGAVNTIKNVRGR
                     LIGTNTDVSGFIRSLETLNFSPKHKSIALLGAGGSARAVLAGLADAGASCIFIHNRTV
                     GRAESLVTEFSQKFPDTQLKSVSVKTILETPLDLLVNTTTVGMETDSSPLDLSQCRKI
                     KHLADLIYSPPQTRLLKQAEELGIPAINGMGMLLYQGCDAFTFWTGQPAPETVMQEQL
                     LNLV"
     gene            complement(6172..6441)
                     /locus_tag="EYO68_02325"
     CDS             complement(6172..6441)
                     /locus_tag="EYO68_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIC06670.1"
                     /translation="MVSQDILIINRLGLHARAAAQLVRMANEYPCDIRLTMDERNSDA
                     KSVMEVLMLGATEGTTLKLSADGKKEDEALKSIVDLFAARFNELE"
     gene            complement(6445..7608)
                     /locus_tag="EYO68_02330"
     CDS             complement(6445..7608)
                     /locus_tag="EYO68_02330"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIC06671.1"
                     /translation="MSEMLFTSESVTEGHPDKISDQISDAILDAMLDQDTASRVACET
                     LVTTGMVVVAGEVTTKAWVDMQKVVRDTVQEIGYTDSNMGFDFNTCAVLISLDKQSPN
                     IAQGVNEGEGAHTEQGAGDQGLMFGYACDETPELMPLPIQLAHRLTERLSHVRKDGTM
                     DYLRPDGKSQVTVRYVDGMPKSVDAVVISTQHAEEVSQSQLHEDIKKNVISHVIPEKY
                     LNDSTQYHINPTGIFVIGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRS
                     AAYAARHVAKNVVAAGLASRCEVQLAYAIGVAEPVSVSVETFGTNKIDESKIIELIRN
                     NFELKPAGLIRALNLLRPIYQKTAAYGHFGREIPEFTWEATDKANELKIQAGL"
     gene            complement(7704..8552)
                     /locus_tag="EYO68_02335"
     CDS             complement(7704..8552)
                     /locus_tag="EYO68_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06672.1"
                     /translation="MPKKLPKFILFLGLLFFIAGCSATKLFYGYGDSIASWQLDSYFD
                     LTNEQEEWVEERMRMHLEWHRNEELPRYRRFLLDIQKSAKDGLTMSELDEGFSRFGTK
                     VGRIFERLIPDAALFLSEISPEQINNLERVMAEENEEMMKRMENQQERLQERHENFWE
                     QMEDWFGEFSISQREQITELQTKWFTESPDPSAERMERRRKSQPQFLALLRSVPEKKE
                     LEKWFRQWFLSWEGETNPARKARIIRNKKRILLIDAILTPEQRLHAVRELDDWVEILE
                     EKIENP"
     gene            8716..>10712
                     /locus_tag="EYO68_02340"
     CDS             8716..>10712
                     /locus_tag="EYO68_02340"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02754.14,HMM:PF13183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIC06673.1"
                     /translation="MNPWLMTLVMLVAWGVFALQITVKLGALTKMAPENRLNEISRRI
                     GRLFKIGFGQHKLIGRSRERGSGIMHAFIFWGALLVGVRELTLMGEGFVSGFQEFLPL
                     LGSDSWLGFAYISVYNVGEVVVLTMILVALYRRLVSKTARLDLNWEGIYVLLFIGMIM
                     LTDLLFDAARLNLIEHYEQQLHFFESTVYGSEWEWAPFTVLLASVLNGLGEGANAFFY
                     HFSYWGHVASMLIFVNLLIHTKQFHEMTALPNVFFGSLDYPHSIPPLADLEDESAWEE
                     GRIGVSKLEHLTWKQGLDLFSCTECGRCHDVCPTFVTDKPLTLKRFNQSLLQHLRNEE
                     STLFRTGSTSEDKTLVGEIIRPETLWACTTCRACEEVCPVSIEHVPRILGLRQNQTMM
                     EEAYPPEMASTFKGLERNFNPWGIGFDQRADWAEGLNIPLMADTKSETEGQPPEIDVL
                     MWVGCAGSFDSRNQKIARATATLYQKAGVKFAILGSEEKCTGDLARRSGNEMLFQMLA
                     GENVETLNNYKIKKIVTACPHCLNSIKNEYPQLGGEYEVFHHSQFIAKLVDEGRLPVS
                     SNLKDTITYHDPCYLGRYNKEYEAPRNLLKHAAGEPMREMKRHGSESFCCGAGGARMW
                     MEETIGSRINENRTEEAIQTGASTVATGCPFCMTMISDGIAA"
BASE COUNT         2754 a         2510 c         2292 g         3056 t
ORIGIN      
        1 gtgccttagt gccttagtgc cttagtgcct tagtgcctta gtgccttagt gccttcttta
       61 ccttttttaa aactttgaaa cacacgcctg cattcggcat ccttgtcctt cattttttga
      121 ttttcgctat agttaatcaa aatttgaatc cacatccgga tatgcttgac cactgggtct
      181 ggtcgcgcta cctgagcttt tcttattatg agcatccacc gatgattgcc tggctcattc
      241 gtggtataac cttcattggc ggaaataatg aaactgctct tgagctcggt tcacaactgg
      301 ttactctaag catactgact ttagtctatg ccggcacatt tcgtttgtac ggaattaaac
      361 ctgccctggt aaccctgctt attttatgca gtatgccgta tttcactctg ggcagcattt
      421 ttctgcacat cacccagcct tttttgattt tttggattct ggctcttttt ctgctgattc
      481 gttttcatca gcagcctgct aataattggc tgctttggat tggagttgcg gctggatttg
      541 gtgcactttc aaagtacata atgctgcttt tttacgcagg actttttcta catctgctgc
      601 tttaccacaa aacacgtaaa gatgtcttga atccctggtt ttattttgca ggaatgctgt
      661 cactgctcat ctttattccg gtaattatct ggaatgcaca acacgactgg gtttcattcc
      721 gctggcagct ggaaaaaggt acttccggag cagattttgg tgaaaacaca ttggctttta
      781 cagttggaca tttgctcctg ttttctccac tttgggcgct gatgggaggc ctgggtatct
      841 ggtggctgcg ggaccgtctg gttgaagcaa gaagtcccga atcagtgata acggtgattt
      901 ctatctttcc gctgctcttt ttcacagtca tgagtctcaa aggaaccatc tcggatccac
      961 actgggcaaa cctggcatat ctaggaattg caattatgct gggtaaggaa ctgctaaggc
     1021 gtttccaaaa gaaaacactt tatgttttat tggcatctgg attattaatc aatgtggccc
     1081 tgaccggaac tgttgttact cacgcactaa atccattgtt tgactggatg ccctatgaac
     1141 tgaagaacta tgattattta aagaataata atgtcccaca ggctactttg gaaaaactga
     1201 ggaataatga cgagcgtctc tacagcacgg gtcagtatcg cctgcattta aagcaaatcc
     1261 tttcaccggc tgagattgag gaatacggcg gattaatcca aaaaaccgcc atggatattt
     1321 cctctgaccg gctgaccagg gttcttgaat gggacaaaac tggtgaacag ctgcagcagt
     1381 tgctggttca aaatggaatt cctcaaattg ctttcatagt ttcacgtgag tatcagttaa
     1441 gcggtgcttt gtctttctat ctgccacacc aaccctggcc gcacagtatt gaaaaaccgg
     1501 aacgtaattt gtggagtccg ttggatgaag taaaaatggg ccccagtatt ttcgtttgtg
     1561 aattacagga atgtcagggt ggggtagtcg atttttatga acggtttgaa atgccgctgc
     1621 gttatttagg agaaatcgaa acccgcaggt atgacagaat ggttcgcaat ttacaggtat
     1681 atgagctcat tccctgagct tccccccccc ctaccggttg atgttcatac ctttgatcca
     1741 tgaagcggaa cattttggta tgtaatggtt ccactctaaa tggagnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnaatgc atcatcgagt tcaagatgca gatcggctgc
     1921 atcaatatct tttatttgtt caattttcat ccgtgctttc gaagcaattt tctgggcatc
     1981 acgaatacgc atttccacat caagaagaat gttaacctgc acagtcagtt cctgattcag
     2041 gtagtggcag taaatgtggg ttatgccctc aatttcagga attacactca aggctttttt
     2101 gacatcattt tctatttcac tttgaggccg catcagcact attttttgcg gatcttccgt
     2161 gccttccgcc tcaaaaaaat catcttctgc atcgacatgc acgagtactt cgttaacagc
     2221 aggcaacttc tctaaaatac ccaaacgcac tctttccgcc acctgatgtg ctgcggaaat
     2281 gctcatcatg ctgtccactt caatgtgtaa atccaccagc agatgcggcc ccattcttcg
     2341 tgcccgcatt tcgtgataat gatccactcc ttcgattcca gacaggatct gtcctaactc
     2401 actgatgact tcttcctccg ccgtctcatc cgtcagttcg cgtatacttt cataaccaat
     2461 gtttattcca gtttttataa tcagacctgc aaccagtact ccggcgattg gatccattaa
     2521 tggaagtccc cattgcgctc ctccgattcc gacgagtgcc gccacagaag atattgcatc
     2581 cgagcggtga tgccacgcat ttgcgagcaa aatgcggctt ccggttcgcc tggcaaccat
     2641 tcttgtggca tgataaagca cttctttaac ggcaattgca attcccgcca tccaaagtgc
     2701 aattgttcca ggcacctctg gcgaatccat tttattaaaa acatgccagg ccactccgat
     2761 tccagtaaat agaagtagaa gcgcaacaat aaaagtgccg acagtttcaa attttccgtg
     2821 tccgtaagga tgatttttgt ctttcggaat tcctgacatg cgcaccgccc agagcgtgat
     2881 cccgtcagaa agcaaatccg aaacagaatg accggcatcc gcgatcaagg ctgatgaatt
     2941 tgcaataaag ccgatgaatc ctttggcgag tgccaaaaga agatttgcca gcagaccaag
     3001 ccaggtaact tttaaggcat ctttttccgt gagatcagtt gtttgcattt cttaaaaatt
     3061 attttttagt tcccgcaatg tccgaaatac ttctttttct gaaagattat gtccgggttc
     3121 cctggacgta acggtttcgc aaatttcggc tgaggagcag cgcataaacg gatttgtccg
     3181 gcgctcctct gccaaagtcg tgggagtgga aaatttaccg gatgaacgca gagccgtaac
     3241 ggcagcaagt tttttattaa tttccgtatt tcctgtttct acgctcaaag caaagcgcaa
     3301 attggctgca gtgtattcgt gtccgaaata cagttcagta tcgtccggaa gtccaccgat
     3361 ttgaatatta agcgaatcat gcatttgctc aggtgttcct tcaaaaagac gtccgcaacc
     3421 tgcggcaaac aaagtgtctc cggtaaacgc ggtcttgcca aacacaaaag ttatggctcc
     3481 ggaagtgtgt cccggattgt gcaaaaaagt tccggtttgc tccccaaaac gtacctcatc
     3541 tccgcttttc aaaaatacag tttgttccgg aatcctcccg cggtcggatt catggccgta
     3601 aattattaac tccggatatt ttgcctttaa ctctttattc ccacccacat gatcccaatg
     3661 gtgatgcgtg ttaagaatcg ttgtcaattc cacgccctgc tgctcaattg ctttttgcac
     3721 aggagctgct tctgatggat caacaacggc tgcctgctgc gtttttgggc aaatcaacaa
     3781 atatgcgaaa ttgtcctcta aacagggaat agttactatt tccatgaaaa ttccttaatt
     3841 tggatgtttt ggtttaactg atactacttt tgtttgcagg gtaccatctt ccattcgcac
     3901 ctgcaattta tctccggaat gaacctcttt tacagatcgt aatgatttac ctttgttatc
     3961 cagcgttaca ctgtaacctc gatgaagaac tgaaagcggg ctgaggctgt ccagcaaatc
     4021 catctgctgc tgcaccgagt cgctcagctt gatttgcaaa agaccaatgg cgcgtgttaa
     4081 acgatcctgc agaaggttca ggctatgctg ctgtgtttcc aattcacgtc cggggctaag
     4141 cagaaaaagt ttttccgcaa taccggaaat ccgattacgc aaattttcca gctgacgctt
     4201 tgttctttcg cgcagcaaag aatccaggtc atccacgcgc tgagcatgtt gctgcagcat
     4261 ggcatcaggc gaagctaatc tcaggcgtac agcttcaaca tattccctga gccggtcaag
     4321 ctgctgctgc attgttcgct gcagccgctc ctgtttttga ctgacagtat gctctaaatc
     4381 ctctttttta ggcacagcca gttccactgc tgcagacgga gttggtgcac gcagatcact
     4441 gacaaaatcg gcaatcgtaa aatcggtttc gtgtccaacc gcagaaatga caggaatatc
     4501 agatgaaaaa atcgcccttg ccagtttctc atcgttaaat gcccacaaat cttctataga
     4561 accgccgcct ctgccgacaa tcaaaacgtc tatctgctgt gaattacgaa gagtgttcag
     4621 gtgctgaatt gcttccacca gacttggtgc tgccatttca ccctgtacct gaactggaaa
     4681 aaacaaaacc ggaattccat caaaacggcg ctccagaaca gtcagcatat catgaattac
     4741 tgcacccgtg ggtgaagtca caattccaat tcttcgaggt aaaaaaggta tggactgctt
     4801 gtgttccgca gcaaacaagc cttctgcatc aagtttggct ttaagttgtt caaaggccag
     4861 ctgaagagcc cccaagcctt ttggttccat tgaggaaacc atgacctgat attcgccgcg
     4921 ctgctgataa acagtgaggt gtccccgcac cactacttcc aaaccatctt ccggttcaaa
     4981 acgcaaaccg gctgcagcgt tccggaacat cactccacgt atttgtgact gatcgtcctt
     5041 gagactaaaa taccaatgtc ccgaggcagc cctctttaca tttgacagct caccttccac
     5101 ggtcaatgag cgaaatttgc cttccaacag cagcgaaatc tggcgggtga gctgtgaaac
     5161 tgtgagcggt tttttttcct gcttagatgc cggattctgg ggatcctgag ttctttgaga
     5221 aatatgggga attgaaggat ttgaaatcgg cgcttctttt ttggagtttt cgtctttttg
     5281 gggagaatct acttcaaaag gaatctgcat gatattttcc gaataaaatc actcaaacca
     5341 ggttaagcag ctgttcctgc atcactgttt ccggagcagg ttgacctgtc caaaatgtaa
     5401 atgcgtcaca accctggtaa agcaacatgc ccataccgtt tatggcaggg atgccaagct
     5461 cttctgcctg cttgagaagt cttgtttgag gaggactgta aatgagatca gccaagtgct
     5521 ttattttacg acattgactc aaatccaatg gacttgaatc tgtctccatt ccgacagtag
     5581 tcgtgttaac cagcaaatct aaaggagttt cgagtattgt ttttacagaa acggatttta
     5641 actgagtatc cggaaatttt tgagaaaatt ctgtcaccag gctttctgca cgtccgactg
     5701 tacgattatg aataaaaatg caggatgctc ctgcatctgc aagtcctgca agcacggcac
     5761 gggcagatcc acctgctcca agcagagcaa tcgatttgtg ttttggagaa aaatttaatg
     5821 tttccagcga gcggataaat ccggaaacat ctgtgtttgt tccaatgaga cgtccccgca
     5881 cattttttat cgtgttgact gcaccaatca gttgcgtaag cggtgagatt tcatcaagac
     5941 aggaaattac agagctttta tgcggaactg taacattgac tccatgaata ttcaaggccc
     6001 tgattccagc aattgcctgc gggatctgct ccggttttac tgaaaatgca agataacggg
     6061 catctatttt caatgcgtca aatgccgcat tttgcatcag cggagaaaat gagtgttcca
     6121 caggacagcc gatgattccg tatatcctgg tcctgccgct tagtatttcc gtcattccaa
     6181 ctcattaaaa cgtgctgcga acaagtcaac aatagatttc agggcttcgt cctctttttt
     6241 tccatctgca cttagtttga gagtagtgcc ttcggtggct ccaagcatca gtacctccat
     6301 aacagatttt gcgtcagagt ttcgctcatc catagtcagg cgaatgtcac aaggatattc
     6361 attggccatt cttaccaatt gagctgcagc acgtgcgtga agcccaagac gattaatgat
     6421 caagatatcc tgacttacca tgaattataa tccagcctgt attttcaatt catttgcctt
     6481 gtccgtcgct tcccaggtga attccggaat ctcacggcca aagtgtccgt atgcggcagt
     6541 tttttgataa atcggtcgca gcaggttcaa ggcccggatc agtcctgccg gttttaattc
     6601 aaaattattg cgaatcaatt caataatttt tgattcatca atcttgtttg ttccaaaagt
     6661 ttcaacagaa acagaaaccg gctcagcaac tccaatcgca taagccaatt gaacttcaca
     6721 gcgtgaggca agtcctgccg caactacatt ttttgctaca tgacgtgctg catatgcagc
     6781 cgaccgatca acctttgaag gatcttttcc tgaaaatgcg cctccaccgt gacgtcccat
     6841 tccaccgtaa gtatcaacaa taattttgcg tcccgtcaaa ccgcagtcac cctgcggacc
     6901 acctatcaca aagatacctg taggatttat gtgatactgt gttgaatcat tcaggtattt
     6961 ctcaggtatc acatgactaa tcacattttt ctttatgtct tcgtgcagtt gagactgact
     7021 cacttcttcc gcatgctgag tactgataac cactgcatcc acagattttg gcatgccgtc
     7081 aacataacgg acggtcacct gcgatttccc gtccggacgt aaataatcca ttgtcccgtc
     7141 tttacggaca tgggagagcc gctcagtcaa ccgatgtgca agttgaatag gaagaggcat
     7201 cagttccgga gtttcatcac aggcatagcc aaacatcaat ccctggtcac ctgcaccttg
     7261 ttcggtgtgt gcaccttcac cttcgttgac accctgtgct atgttaggag attgtttgtc
     7321 caggctgatc agtactgcgc aagtattgaa atcaaagccc atgttggaat ctgtataacc
     7381 aatttcctgg acagtatcac gcaccacttt ttgcatgtca acccacgctt ttgtagtaac
     7441 ttctccagcc accacaacca ttccggtggt cacaagggtt tcacaagcca cccggctggc
     7501 ggtatcctga tccagcattg catcaagaat tgcatcagaa atctgatcag aaattttgtc
     7561 cggatgacct tcggttacac tttcggaagt aaatagcatt tcactcatag ttttttatgt
     7621 ttaataagtt gaaaattgta aaattttgaa aattagaatc gttcattttt caagaaaagt
     7681 ccagccaaat gactattaaa actctaaggg ttttcaattt tttcctccag tatttctacc
     7741 caatcgtcta attcccgaac cgcgtgcagt cgttgctcag gcgttaaaat agcatcaatc
     7801 agcagaattc tctttttgtt tcgtataatt ctcgccttcc tggcaggatt agtctcgcct
     7861 tcccaactta gaaaccattg gcggaaccat ttttcaagtt cttttttttc aggaacagaa
     7921 cgtaacaagg ctaaaaattg gggctgcgac tttcggcgtc gttccatacg ttccgcagaa
     7981 ggatccggag attcagtaaa ccattttgtc tgcagttccg tgatttgttc tcgctgactt
     8041 atactgaatt ctccaaacca gtcttccatt tgctcccaga aattttcatg tctttcctgt
     8101 aaacgttcct gctgattttc cattcttttc atcatttctt cgttttcttc ggccattacc
     8161 cgttccagat tgttaatctg ctcaggactt atttcagaaa gaaataatgc tgcatccgga
     8221 atcaaacgtt caaatatccg acctacttta gttccaaaac gggaaaaacc ttcgtcaagc
     8281 tcactcatcg tgagtccgtc ttttgcactt ttttggatat ctaacaggaa gcgtctatag
     8341 cgcggcagtt cctcattacg gtgccattca aggtgcatcc gcattcgttc ttccacccat
     8401 tcttcctgtt catttgtgag atcaaaatat gagtccagct gccatgaagc aattgaatcg
     8461 ccataaccgt aaaacaattt ggttgcacta caaccggcaa taaaaaagag caaacctaga
     8521 aataaaataa attttgggag ttttttcggc atttatattt tgaagcaggg atgtagtgga
     8581 ctaccataac accagatgtc ttaaaagaga agtaatctct ggaaattgcc ttgccaatga
     8641 aagcaattcg ttctattttt ggaaacagat ttttattaaa attttatttt tcactaactc
     8701 aattaaaagg taaaaatgaa tccatggtta atgactttgg taatgctggt tgcctgggga
     8761 gtatttgccc tgcaaatcac cgtcaagctt ggtgcactca ccaaaatggc acctgaaaac
     8821 cgcctgaacg aaattagccg aaggatcggc aggcttttca aaattggttt cggtcagcat
     8881 aaattaatcg gccggagccg cgagcgtggt tccggaataa tgcacgcatt tatcttctgg
     8941 ggtgcattac tggttggggt gcgtgaatta accttgatgg gcgaaggctt tgtcagtgga
     9001 tttcaggaat ttttgccctt gctcggatca gactcctggc tgggctttgc ttatatttcg
     9061 gtttataacg taggcgaggt ggttgtattg accatgattt tagttgcact ataccggaga
     9121 ctcgtgtcca aaacagcaag acttgatttg aattgggaag gcatctacgt tttgcttttc
     9181 atcggtatga taatgctgac cgacttgctg tttgacgccg cacgcctcaa tttgatagaa
     9241 cattacgaac agcagctgca cttttttgaa agcactgttt acggttcaga atgggaatgg
     9301 gcccccttta ctgtcttact tgcttcagtg ctgaatggac ttggagaagg cgccaacgcc
     9361 tttttctatc atttcagtta ttggggacat gtggcatcaa tgttaatttt tgtgaatttg
     9421 ctgatccaca ccaaacagtt tcacgaaatg actgcacttc caaatgtgtt ttttggctca
     9481 ctggattatc cgcattccat tccgccgctg gcagacctgg aagatgagag cgcctgggaa
     9541 gagggtcgta tcggagtcag taaactggaa cacctgacct ggaagcaagg gctggatctc
     9601 ttttcctgca ctgagtgcgg gcgctgccac gatgtctgtc ccacatttgt aaccgataaa
     9661 cctctcacat taaaacgttt caatcaatcc ctgctgcagc atttacgaaa tgaggaatcc
     9721 acgctgttcc ggacaggatc gacctcagaa gataaaacac tggttggaga gattatccgt
     9781 ccggaaacac tttgggcctg tacgacttgc cgggcctgtg aagaggtttg tcctgtttca
     9841 attgagcatg ttccccgtat cctgggttta cgtcaaaatc aaaccatgat ggaagaggca
     9901 tatccgccgg aaatggcttc gactttcaaa ggccttgaac ggaactttaa tccatggggt
     9961 atcggttttg accagagagc ggattgggcg gaaggtttga atatccccct gatggcagac
    10021 acaaaatccg aaacggaagg tcaaccgccg gaaattgacg tgctgatgtg ggtcggatgc
    10081 gccggttctt ttgacagccg caaccagaaa attgcccggg ctacagccac gctttaccaa
    10141 aaagccggtg taaaatttgc gatcctgggt tcagaggaaa aatgtaccgg tgacctggca
    10201 agacgttccg gaaacgaaat gctgtttcaa atgctggccg gtgaaaacgt ggaaaccctg
    10261 aataattaca agatcaaaaa aatcgtcact gcctgtccgc actgcctgaa cagcatcaaa
    10321 aacgaatatc cgcagctggg tggcgagtac gaggtttttc atcattcaca gtttattgcc
    10381 aagttggtgg atgagggacg tttgccggtt agcagtaatc tcaaggatac aatcacctac
    10441 cacgacccat gttatctggg gcgctacaac aaggaatatg aagcacccag gaatttatta
    10501 aaacacgctg caggcgagcc gatgagggaa atgaagcgtc acggcagcga aagcttttgc
    10561 tgcggagccg gtggtgcccg gatgtggatg gaggagacaa tcggcagccg catcaacgaa
    10621 aaccgcacgg aagaagccat ccaaaccgga gcctcaacag tagctaccgg atgtccgttc
    10681 tgcatgacca tgatcagtga cggaattgcc gc
//
LOCUS       DTVD01000062            2709 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15499_length_2709_cov_262.077619, whole genome
            shotgun sequence.
ACCESSION   DTVD01000062 DTVD01000000
VERSION     DTVD01000062.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15499_length_2709_cov_262.077619"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            58..2553
                     /locus_tag="EYO68_02345"
     CDS             58..2553
                     /locus_tag="EYO68_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patched family protein"
                     /protein_id="HIC06674.1"
                     /translation="MTNLSNRSMKQMIQKIESRFEQMGRWIHVHPKRVILVMLLFFAI
                     LASNLPSIQVDTSTEAFFSENDQTRITYDKFREQFGRDEIVLALIHPKKVFDIEFLKK
                     LKAFHEDLEEEVPHLDEVQSLINVTAMRGEGGELIIEELMADWPEDDAGVKDLKERVL
                     RNKFYRNFLVSEDGQYTTVVVRSNAFSDVDKDMNQEEILEEGFSDEEDTSLSDNDNPQ
                     LLTEEQNSEFVEAIQALAERHRNSEFPIDLGGSPVMVHDLKKAMFSDMPVFVLASLGV
                     IALLLVILFRRLSGLVLPILTVIFSLLAALGLVAATGTKMTIVMQILPSFLLAVGIGY
                     SVHLLVIYYRHLSEHGDKGEAIAFAMGHSGLAILITSLTTAGGLLSFVPVKVAPVSDL
                     GLFGAAGVMLCVFFTLVLLPALLSVLPEGKPAVVAEKLYMQETSRPQLSFADRMLKGC
                     GNFAVNQPWTVIVISVLIALMSSFGASQLRFSHNPIAWLPDNHSLRNATDAINDHMKG
                     SAAIELVVERGEENAVKDPEFMKRLDEFNRFSEGTSYNRISVGKSSSIVDMVKEINQV
                     LNEDREEFYRVPLDRGMIAQELLLFENGGTDDLESLVNTPFSKARVTLKTTWVDANQY
                     TGLLLKLERKIEELFGQEKSYVVTGLIPIMVKTITFLMEGMLISYLIAGVVITLLMIF
                     LLADFRLGLWSMIPNFLPVLVGLGVMGMLDLPLDAMSILVGSIAIGLAVDDTVHFMHN
                     FRRNQHIHQDVQVAVEKTLTSTGRAMLLTTIVLSAGFFIFIISSMNNLISFGLITGLT
                     IIIALFGDILLAPAMMALIYKNRPSNSIKKETL"
     gene            2550..>2709
                     /locus_tag="EYO68_02350"
     CDS             2550..>2709
                     /locus_tag="EYO68_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIC06675.1"
                     /translation="MMFIKIISNTINITIFSILISGTIMLPEKVFALNGREIMEKVNA
                     RDTGDRSIT"
BASE COUNT          721 a          602 c          639 g          747 t
ORIGIN      
        1 tggacccgaa aatagcaatt aaggaggcca gggtattatt acgacaaaaa ttatttgatg
       61 accaacttga gtaacagatc catgaaacaa atgatacaaa aaatcgaatc gagattcgaa
      121 caaatggggc gctggattca tgtccatcca aaacgagtga ttctagtaat gctgttgttt
      181 tttgcaattt tagcttcgaa tctaccttcg atacaggtcg atacgtcaac cgaggcattt
      241 ttcagcgaaa acgatcaaac caggattacc tacgacaagt tccgtgaaca attcggccga
      301 gacgaaattg tactggcact catacatccg aaaaaggttt tcgacattga atttttaaaa
      361 aagctgaaag ccttccatga ggatcttgag gaagaggttc cacatcttga tgaagtacag
      421 agcctgataa acgtgactgc catgcgtggt gagggaggtg agttgatcat cgaggaactg
      481 atggcagact ggcctgaaga cgacgcagga gtcaaggatc tcaaggagcg agtactcaga
      541 aacaagttct accgcaactt tctggtttca gaggatgggc aatacacgac tgtggtggtt
      601 cgctctaatg ctttttctga tgtggacaaa gatatgaatc aggaagaaat actggaagag
      661 ggattctcag acgaagagga tacttcactt tccgataatg ataaccctca gttgctgacc
      721 gaagagcaga acagtgagtt cgttgaggcc attcaggctt tggctgaacg tcaccgcaat
      781 tctgaattcc cgatcgacct cggcgggtct ccagtgatgg ttcatgatct taaaaaagcg
      841 atgttctccg acatgccagt tttcgtattg gcatctctgg gggtgatcgc cctacttctg
      901 gtaatactct tccgccgttt atcgggtttg gtacttccta tactaacagt gatattttct
      961 ttgttggcgg ctctgggtct agtggccgcc accggaacaa aaatgacgat tgttatgcag
     1021 atactccctt ctttcctact tgccgttggg atcggttatt cggtccattt gctggtcata
     1081 tattaccgac acctgagcga gcacggcgac aaaggggagg cgattgcttt tgcaatgggc
     1141 cattccgggc tggcgattct tatcaccagc ctgaccacgg cggggggcct cctttccttt
     1201 gttccagtaa aggtggcacc agtttcagat cttgggttat ttggggcggc aggcgtaatg
     1261 ctctgtgttt tcttcaccct ggttctctta ccagccctgc tttcggtgct tccagaagga
     1321 aaacctgcag ttgttgcaga aaaactgtat atgcaggaaa catctcgacc ccaactctca
     1381 tttgcagacc ggatgctcaa aggttgtgga aattttgcag tcaatcagcc atggactgtg
     1441 atagtgatca gcgttctgat agctttgatg tcatcgttcg gtgcctcgca gcttcgtttt
     1501 tcacataatc ccattgcctg gttgccagat aaccactctc tgcgaaatgc gaccgatgca
     1561 atcaacgatc atatgaaagg cagtgcagcc attgaactgg tcgtcgaacg tggtgaggaa
     1621 aatgcggtca aggatccgga attcatgaaa cgtcttgatg agttcaatcg tttttccgaa
     1681 gggacaagct acaacaggat ttctgtcggt aaatcttcat ccattgtcga catggtcaag
     1741 gaaatcaacc aggtactaaa tgaggaccga gaggaattct accgggtacc tctggaccga
     1801 ggaatgatag cgcaggaact cctgctgttc gaaaacggtg gtactgacga tctcgaaagt
     1861 ctggtcaata cacctttttc caaggcacgt gtgacgctca agacaacttg ggtggatgca
     1921 aaccaatata ccggacttct gctcaagctg gaaagaaaga tagaggaact ttttggacag
     1981 gaaaagtcat atgtcgtcac cggactcatt ccgatcatgg taaaaacaat cacctttctt
     2041 atggaaggca tgctgatcag ttacctcatt gcaggagtag tgatcactct gctgatgatt
     2101 ttcctgcttg ctgattttcg tctggggctc tggagcatga tcccgaattt tctgccagtc
     2161 cttgtagggt tgggtgtgat gggaatgctc gatctgccct tggacgcgat gtcgattctt
     2221 gtaggcagca tagcgattgg acttgctgtc gacgataccg tgcacttcat gcacaacttc
     2281 cgtcggaatc aacacattca tcaagatgta caggtggctg tggaaaaaac actaacttca
     2341 actggtcggg ccatgcttct taccactatc gttctttccg caggtttttt catcttcatt
     2401 atttcgtcta tgaacaacct tatttctttt ggtctgatta caggtttgac cattatcatc
     2461 gcgttgtttg gagatatcct gcttgcccca gcaatgatgg ctttgatcta caaaaatcga
     2521 ccttccaact caataaaaaa ggagacacta tgatgttcat taaaataatt tcaaacacaa
     2581 tcaatattac cattttcagt attctaattt ctggaaccat tatgcttcct gaaaaagtat
     2641 ttgccctcaa cggacgcgaa atcatggaaa aagtcaatgc ccgtgacaca ggcgaccgct
     2701 caattactg
//
LOCUS       DTVD01000063           10641 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1563_length_10641_cov_261.180238, whole genome
            shotgun sequence.
ACCESSION   DTVD01000063 DTVD01000000
VERSION     DTVD01000063.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_10641_cov_261.180238"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="EYO68_02355"
     CDS             complement(<1..1081)
                     /locus_tag="EYO68_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIC06676.1"
                     /translation="MSKAMDDFRLKLGGREYVPIIVGGMGVDISTAELALEAARLGGI
                     GHISDAMVPTVSDRRFNTKFVQEKQKKYKYNIGNTDKSAVKFDLERLAEAQRLFVSRT
                     MESKKGDGAIFINCMEKLTMNSPRDTLQARLLAAMDSGIEGITLSAGLHLGSMELMKD
                     HPRFHDVLIGIIVSSARALRPFLKRAAKYKRLPDYITVEGPLAGGHLGFGADDWQEYD
                     LKTIVNDVLVFLKENELNIPVVPAGGVFTGTDAVEFLESGSSAVQVATRFTVTHECGL
                     PEKTKHHYLEAVEDDIVVNTISPTGYPMRMLRESPGIGSGIRPNCEAFGYILDSKGHC
                     QYVDAYNRELEKNPENISVQDKTCLC"
     gene            complement(1135..>1335)
                     /locus_tag="EYO68_02360"
     CDS             complement(1135..>1335)
                     /locus_tag="EYO68_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC06677.1"
                     /translation="AIRTLSSAGIKDFPELMRVAGEHSAMKRTATQSEVAQTAVFLAS
                     EASSGITGQVIYVDCGYSIMAN"
     assembly_gap    1336..1435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1436..2275)
                     /gene="psd"
                     /locus_tag="EYO68_02365"
     CDS             complement(<1436..2275)
                     /gene="psd"
                     /locus_tag="EYO68_02365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052016.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIC06678.1"
                     /translation="MAIPGFGHPPAYYFMWILPRNLFSRLCGIVADARIPRPLLTPLI
                     KLFSWKFGVDLTEAAQPISEFRTFNEFFTRHLLPDARTVDPDPDVIVSPVDGFIGEFG
                     TIKKGRLIQAKGLDYRLADLLEDSERTEQYDGGVFITIYLAPHNYHRVHSMVEGEVQE
                     FFYITGDLWTVSPMGVHHVPGLFARNERLTTFLATDKGECALVKVGATVVGRIRVCYH
                     DLVSNRSGAKNQQIVLKTPFQVNRGEELGLFELGSTVICLFPKGQIELGELEAEQKVY
                     LGQA"
     gene            complement(2275..4110)
                     /locus_tag="EYO68_02370"
     CDS             complement(2275..4110)
                     /locus_tag="EYO68_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIC06679.1"
                     /translation="MKRENILIILSFVVLAFFLARMFNSGDITSGPQRSQELIYSDFK
                     ALVAAGDVVNAQIVGDSLIEGFTQGGLGYRTYIPAEDPSVIDFLRLYQIPINYVPEKS
                     LPWYLSILINWGPFILIFGIWMVLMRRMQGGGAGGGLFSIGRSRAKKMEPDEMKVTFD
                     DVAGIEEAKDDLRETVEFLKDPAKFRKLGGRIPKGMLMSGSPGTGKTLLAKAVAGEAE
                     VPFFSMSGSDFVEMFVGVGASRVRDMFEQGRQNSPCIIFIDEIDAVGGSRGSGMGGGN
                     DEREQTLNQLLVEMDGFDGMEGIIVIAATNRPDVLDPALLRPGRFDRQVTIPLPDVKG
                     REEILKIHAKKVQMADGVDMSIIARGTPGFSGADLKNLINEAALGGARNNKTELNLAD
                     FEWARDKVMMGTERRSMVMSEKEKRNTAYHEAGHALVSALLPKSDPIHKVTIVPRGRA
                     LGMTAYLPEKDNHSYDLEFLSNRITIAMGGRAAEEIIFDEVTTGASNDIQQATDTIRN
                     MICRWGMNKSFGPIVFGSDNRQYVLGRNIGKDREYSEKTASEIDKEMRKTIKFHDEKA
                     KKLLRDNIDVLHKIVEVLIKEETVEGSYIMDLLGQNKSKVEPVGG"
     gene            4297..5682
                     /locus_tag="EYO68_02375"
     CDS             4297..5682
                     /locus_tag="EYO68_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIC06680.1"
                     /translation="MNHFILFSQLKRFLHESSRTLKLGLPVIIAQLLQTLMQFVDTVM
                     AGHVSSKDLAGLAIATALYHPVFLLMLGILIALGSIIAQLFGAVKPAEIVTNVIQGLW
                     LSQVLALVSIVILANPEPVLHFMGYEAKVIQVAGDYLRALCWGMPAVYAYIVLRMFNE
                     GLSITRPNMYFSLIGLAINIVGNYTLMFGHFGFPALGAVGAGWTTSMVHWVMFSVMLA
                     FCLKGSLFKSYRKFLYFSGPVWLHLREILRIGLPNGFSFAAEVGMFAMVSLLMGTFGV
                     SAIAGHQIAINVASLTFMIPMGLSIAITIRVGQAKGRDNCIEARYIGYAGILLCIIVM
                     AVSASIFVIVPEMIVGLYTEDLQVQTLAIQLLYMAAIFQLSDGMQVGALGALRGLKDT
                     KIPLVANVAAYWGLGLPLAYLVGIHFNVGPAGMWVGLITGLSTAAVFHSWRFRILTNR
                     LLILPSSRKFT"
     gene            5892..7502
                     /gene="fliF"
                     /locus_tag="EYO68_02380"
     CDS             5892..7502
                     /gene="fliF"
                     /locus_tag="EYO68_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HIC06681.1"
                     /translation="MAEQTGALSDVRLQFQDFFGSLSLTKRITILVVLGIVLIGMVSM
                     IFVANRPSWAPLYSNVEPGDAAAIVEKLQENQIPYLLAPGGRTIMVEPHKVDQARLTL
                     AKEKVTPGSGVGFLDLFSTPSLGETEFQQGVKFRVAQEGELARLISTINVVKSAKVSL
                     AIPQKTLFSDNQEEPTAAIALNLTSSGAGRKQIETIIHLVASAVEGLSPRNVKITDQS
                     GTLLSKGFSDSSAGGKMNDNYSYKSRFENELEAKIVRQIEEVVGKDRVRVNVSATLRF
                     DRRTIKEELVDPDQTSVVSEQVVDESSTGTRSVPVGPAGVTTNLPEATGREAATVSEF
                     SKKNSTRNFESSRREIVLESAVGEVVRLSVSVLLDNRRSQILDESGISLGRTNTPWTE
                     AEKETIVGLVKAAMGFNEKRGDSVFVGNMPFEAAVEDDKVNEIEATRVRNKFILDIVR
                     YVALGLAILSLIMFVIRPMVQRLSSKPEDLDLLMGMPATIGELEEEELEVPTERESGI
                     PPREKILEIAKQDPLKTSALIHTWLRER"
     gene            7618..9102
                     /locus_tag="EYO68_02385"
     CDS             7618..9102
                     /locus_tag="EYO68_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HIC06682.1"
                     /translation="MLEDSEFKPFNLTDFTVVETLPEDSESLSATQEDGQDGAEVEES
                     EISVRLASFKPLIIDEQGGSEEFDANGSTDLADRRTDAEKIHLSQDFRTADFFQENSL
                     LTNAENFAETIRDGAKLYKTQLLTKIEEQATDTKRIHKKTVAENQEAEEERKKLLSAT
                     EEKVDEIKNEAFNEGFEAGRLKGMQKRYDEAGPLVLQVNSVMEQLNSLRQAVRFQAEK
                     ELVELALQIAKKVVAEEIKLPNDVIKNIVKAALHETEVQGKIYLYLHPDDYEFLLKSK
                     ADLERYLNEEQTLVLRQNPELKPGSIYVESDEEIISRSIEDQFDKLEETLTEQIENRH
                     AQLSEVDLDAHDFSIRTSSDDAANADSAAVHLQDVGEVDSPAETDEKAELKKADPEKS
                     TVKDTEEIAQKTMPVDSSEVDDNEKPVTQSGSVDLREMDETDLSEEAADPALETESIE
                     TEEQEQQTKPVENKEPELETEAAKTEESVPETETDESAELDPPK"
     gene            9206..10594
                     /locus_tag="EYO68_02390"
     CDS             9206..10594
                     /locus_tag="EYO68_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HIC06683.1"
                     /translation="MLSETPASELPVYSNLGTYISEIEKFPAVRKEGRVMQVIGHMVE
                     ATNPGCSVGGMCKIYNPATKSSVTAEVVGFRKDRMLIMPLHNTHGIGPKCRIIPEDRP
                     AMVPVGDGLLGRVIDPLMRPMDGLGELPTSTEVPLYPEVVNPMNRQRIKKPLDVGVRS
                     INACLTCGRGQRIGIMAGSGVGKSVLLGMMARYTDADINVISLVGERGKEVREFIEDN
                     LGEEGMRRSVVIVATSDQPPLLRMRGAYIATSIAEHFRNEGQDVLLSMDSLTRFAMAQ
                     REIGLAAGEPPTTKGYPPSVFALLAGLLERTGTHDGPGSITGFYTVLVEGDDANDPIA
                     DAIRAIVDGHIYLSREIAEKGTYPAVDLLSSTSRVMVDVVGEKHLKLNQIMRRTLATY
                     REAEDLINIGAYVQGSNPEIDYAITKKPLIQEFIQQGMNEHTDLKECEEHILQIFGDR
                     LEEDTTPEENSA"
BASE COUNT         3101 a         2259 c         2243 g         2938 t
ORIGIN      
        1 tgcagaggca tgttttgtcc tgcacagaaa tattttccgg atttttctcc agttctcggt
       61 tgtaggcatc cacgtattga cagtgccctt tgctgtccag aatataaccg aaagcttcac
      121 aatttggacg gattcctgaa ccgattccgg gagattcccg aagcatccgc atagggtaac
      181 cggttggaga aatagtgtta acaacaatat catcctcaac tgcttccagg taatgatgtt
      241 ttgttttttc cggtaatccg cactcatgag taaccgtaaa gcgggtggcc acctgaacag
      301 cagaagatcc ggattctaaa aattccactg catcagttcc ggtaaaaacg ccgccggcag
      361 gaacaacagg tatatttaat tcgttttctt ttaaaaatac taaaacatca ttgacaattg
      421 ttttaagatc atactcctgc caatcatctg caccaaaacc tagatgtcct ccggccaacg
      481 gtccttcaac cgttatataa tccggaagac gcttgtattt tgctgctctc ttgagaaaag
      541 gacgtaaagc tcttgctgaa gacacaatga tgccaatcaa aacgtcatga aaacgggggt
      601 ggtccttcat aagctccatt gagcctaaat gaagtcctgc actcaaggtt ataccttcaa
      661 tacctgaatc cattgctgca agcaatcgag cctgcagagt gtcacgagga ctgttcatgg
      721 taagcttttc catgcagttg ataaaaatcg ctccgtctcc tttttttgat tccattgtac
      781 ggctcacaaa aagacgttga gcttcggcca gtctttccaa gtcaaacttc actgcagatt
      841 tgtccgtatt gccgatattg tatttgtatt tcttttgttt ctcctgcaca aatttcgtat
      901 taaaacgacg gtcagaaaca gtaggtacca ttgcatcgga gatgtgacca attcctccca
      961 aacgtgctgc ttccagagca agttctgcag tagagatatc aacccccatc ccacccacga
     1021 ttataggcac atattcccgt ccaccaagtt tcaagcggaa atcatccatg gctttgctca
     1081 tatcatgctc acttttctta gggtttgctt attaaaaata ttattcagta tttatcaatt
     1141 ggccataatc gaataaccac aatctacgta gatcacttgt cctgtaatcc ccgatgaggc
     1201 ctcacttgct aaaaatacag cagtttgcgc tacttcactc tgtgttgcag tgcgtttcat
     1261 ggccgaatgc tcgccggcaa cacgcatcaa ttcaggaaaa tctttaattc cggcagaaga
     1321 caaagtcctt attgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagcct
     1441 gacctaaata aaccttttgt tctgcctcta actcccccag ttctatctga cctttaggaa
     1501 acaggcaaat taccgtagat cccaattcaa acaaaccaag ctcttcgcct cgattgactt
     1561 gaaaaggggt cttcagaact atctgttgat tttttgcacc agacctgttt gaaacaagat
     1621 catggtaaca aactcgaatg cgtcctacca cagtcgcacc aacttttaca agcgcacatt
     1681 cacccttgtc agttgctaaa aaggtggtta atcgttcgtt cctggcaaaa agtccgggga
     1741 cgtggtgtac tcccattggg ctaactgtcc acaaatcacc agtaatgtaa aagaattcct
     1801 gtacctcacc ttccaccatg ctgtgaacac ggtggtaatt gtgcggggct aaataaatgg
     1861 ttatgaatac tccaccatca tattgttcgg ttcgctccga atcttccaat aagtctgcta
     1921 atcgatagtc gagtcctttt gcttgaatca gtcgaccttt tttaatagtc ccgaattcac
     1981 caataaatcc atctaccgga cttactatga catccggatc cggatcaact gtccttgcgt
     2041 ccggaagcag gtgccgggta aaaaattcat taaaggtacg gaactctgaa atcggctgtg
     2101 ctgcctcagt caggtcaact ccaaatttcc atgaaaataa tttgattaat ggagttaaca
     2161 aaggacgtgg aattcgagca tccgccacaa tcccgcaaag tctgctaaaa agattgcggg
     2221 gcaaaatcca cataaaataa tatgccggag ggtgaccaaa tcccggtatt gccatcagcc
     2281 cccgactggc tcaactttag acttattttg ccccaaaaga tccatgatat acgaaccctc
     2341 gactgtctcc tcttttatca aaacttcaac tattttgtgt aaaacatcga tattatccct
     2401 gagaagtttc ttggcttttt catcgtgaaa tttaatagtt ttgcgcattt ccttgtcaat
     2461 ctctgaagcc gtcttttcac tgtactcacg atctttgccg atattgcgac caaggacgta
     2521 ttgtcgattg tcactcccaa aaacaatcgg gccaaatgat ttattcatac cccagcggca
     2581 aatcatattt ctaattgtat ccgttgcctg ctggatgtca ttgcttgctc cggtagtaac
     2641 ttcatcaaaa ataatttcct ctgctgcacg tccacccatc gcaatggtta tgcggttgct
     2701 cagaaattca agatcatatg agtgattatc cttttccggt aaatatgcag tcattcccag
     2761 ggctcttccg cgaggcacaa ttgttacttt gtgtattgga tcagatttcg ggaggagggc
     2821 cgaaacgagt gcatgtccag cctcatggta ggcagtgttg cgcttctctt tttctgacat
     2881 gaccatcgat cgacgttccg tacccatcat aaccttgtca cgtgcccact caaaatccgc
     2941 caaattcaac tctgtcttgt tgttgcgtgc acctcctagt gcggcttcat taatcaggtt
     3001 ttttaaatct gcacctgaaa atcctggagt accacgagca ataatactca tatcaactcc
     3061 atctgccatt tgcacttttt tagcatggat tttcagaatt tcctctctac cttttacatc
     3121 aggtagagga atggtcactt gccggtcaaa acgtcccgga cgcagcagtg caggatccag
     3181 tacatccgga cggtttgttg cggcaattac gattattcct tccattccat cgaaaccatc
     3241 catttcaacc agcaattggt tgagggtctg ttcccgctca tcatttccac cgcccattcc
     3301 cgaaccccgg ctcccgccga ctgcatctat ttcgtcaatg aaaattatac acggtgaatt
     3361 ttgccgtcct tgctcaaaca tatcacggac cctgctggca ccaacaccta caaacatttc
     3421 cacaaaatca gaaccgctca tactgaaaaa agggacttct gcctcaccgg caacagcctt
     3481 tgccagcaag gtttttcctg taccgggtga tccggacatc aacatacctt ttgggattct
     3541 tcctccgagt tttcggaatt tggctgggtc tttcaggaat tcaaccgttt ctctcagatc
     3601 gtcttttgct tcttcgattc cagcaacgtc atcaaaagtc actttcattt catcaggttc
     3661 catttttttg gcacgactac gtcctataga aaacaatcct cctccagctc ctccaccctg
     3721 cattctccgc atcagtacca tccaaatccc aaaaattaaa atgaacggtc cccaattaat
     3781 taaaatgctc aaataccatg gaagactttt ttccggtaca tagttaatgg ggatttgata
     3841 aagtcgaagg aagtcgatta cacttgggtc ttcagccgga atataggtgc ggtaacccag
     3901 tcctccctga gtgaatccct caattaagga atctcctaca atctgtgcat taaccacatc
     3961 acccgcagca acaagtgctt tgaaatcgct gtaaatcaat tcctgagaac gttgaggtcc
     4021 ggatgtaata tccccgctgt tgaacattct tgccaaaaaa aatgccaaca ccacaaaact
     4081 gaggataatc agaatattct ctcttttcat actaaaaatc cttctgcaaa ataaagatcg
     4141 aagttttgga tgatcgtaaa taacagatac acctaaaacc acaaaaaata aaatttatta
     4201 tataaaaaac aatgtttagt agtacatgat agcatacaac tggtcttaca gttaagtaaa
     4261 ttattttata ctcaaaatca tttcaaacgt tatttcatga atcattttat tcttttttca
     4321 caattaaaac gatttttaca tgaatcttca cgcacattaa agttaggtct accggtaatc
     4381 atagcacaat tgttacagac tttgatgcag tttgtggaca cagtgatggc cggacacgtc
     4441 agttccaaag atttggcggg actggctatt gcaacggcac tctaccatcc agttttttta
     4501 ctgatgttgg gtattttgat tgctttaggt tcgattatag ctcagctatt tggggcagta
     4561 aaacctgcag aaattgttac aaatgtaatt cagggcctct ggttaagcca ggttttagcg
     4621 ctggtgagca ttgtaatttt agctaatccc gagccagttt tgcattttat gggatatgaa
     4681 gcaaaggtga ttcaggttgc cggagattat ttaagggctt tgtgctgggg gatgccagcc
     4741 gtttatgcat atatcgtttt acggatgttt aatgaaggct tgtcgatcac tcgtccaaat
     4801 atgtattttt cgctaattgg actggcaatc aatatcgtag gaaactatac actcatgttt
     4861 ggtcacttcg gttttcctgc ccttggagca gttggtgcag gatggacaac aagcatggtg
     4921 cactgggtta tgttcagtgt tatgctagca ttttgtttaa aaggctcact gtttaaaagc
     4981 tacagaaaat ttttgtattt tagtggcccc gtctggcttc atctgcgtga aattttgcga
     5041 atcggcttac caaacggttt cagcttcgca gctgaagttg gaatgttcgc aatggtttcc
     5101 ttgctgatgg gaacttttgg ggtttcggca attgcaggac atcagatagc aatcaatgtt
     5161 gcttcgttaa catttatgat accgatggga ctttcaatag ccataactat aagagtgggc
     5221 caggcgaagg gcagggacaa ttgtatcgag gctcgatata tagggtatgc tggaatttta
     5281 ctctgtatta ttgtcatggc agtatcagca tctatatttg ttatagttcc ggaaatgatc
     5341 gtaggattat atacggagga tctgcaagtc cagacgctgg caattcaatt actttatatg
     5401 gcagcgatat ttcagttgtc agacggaatg caagtaggtg cattgggcgc actgcgtgga
     5461 cttaaagata caaagatacc gttagttgca aatgtggcag catactgggg cttaggcttg
     5521 cctttagcct acctggtggg aatccatttt aatgttggtc ctgcaggcat gtgggtcggc
     5581 ttgattaccg gcttgagtac tgctgctgtt tttcatagtt ggcgatttcg gattttgacc
     5641 aaccgccttt taatccttcc ttcttcacga aaatttacat gaagaaggta ggattttgcg
     5701 aatgaaatct gattaatata atctttttta ttttatcaaa agacaaaaca gcatttactc
     5761 aatgtttgga atcctaattg caggaaacat gattcaaaat tcttaaaaaa attttatgtt
     5821 tgattcacaa ttttagaata caggcattaa attttcccaa tatcaccaga atactattaa
     5881 acaggaatac tatggcagaa caaaccggcg ctctttcaga tgtcaggtta cagtttcagg
     5941 attttttcgg ttcattaagt cttactaagc gaatcacaat tttggtggtc cttggaatag
     6001 ttctcatcgg aatggtgagt atgatttttg tagcaaaccg accatcttgg gccccgcttt
     6061 attctaacgt ggaaccaggc gatgctgccg ctatagttga aaaactccag gaaaaccaga
     6121 ttccttattt gcttgctcca ggtggaagaa ccataatggt tgagccgcac aaggtagatc
     6181 aagcccgctt aactttggcc aaagaaaagg ttacgccggg aagtggagtc ggatttcttg
     6241 atctgttttc tacaccaagt ctaggtgaaa ccgaattcca gcaaggtgtt aaattccgtg
     6301 tagctcagga aggtgagctg gcgcggctga tttctactat taatgtagta aaatcagcaa
     6361 aagtttcatt agctattcca caaaaaactt tattttcaga taatcaggaa gaaccaactg
     6421 ctgcaatcgc actaaacctc accagcagtg gagcaggcag aaaacaaatt gagactatta
     6481 ttcacttggt tgccagtgct gttgaaggtc tttccccacg aaatgtaaaa ataacagatc
     6541 aaagcggaac gctgctcagt aaaggttttt ctgattcttc tgccggtgga aagatgaacg
     6601 ataattactc atataaaagt cgttttgaaa atgagcttga agcaaaaatc gtcaggcaaa
     6661 ttgaggaggt tgttggtaaa gaccgtgtaa gagtaaatgt ttcagctacc ctgcgttttg
     6721 ataggcgaac aatcaaggaa gaactggtgg atcctgatca aacgagtgtc gtcagtgagc
     6781 aagttgttga tgagtcttca accggaacac gatcggttcc tgtcggacct gccggagtaa
     6841 caaccaattt accagaagca actggcaggg aagcggcaac tgtctcagaa ttcagcaaaa
     6901 aaaattccac gcgtaatttc gaaagcagcc gccgggaaat agttctggaa tctgcggtgg
     6961 gagaagtagt acgcctgagc gtttctgtgc ttcttgacaa caggcgttcg caaatactgg
     7021 acgaaagcgg aatttcatta ggccgtacta acacaccttg gactgaagca gaaaaagaaa
     7081 ctattgttgg tctggtaaaa gcagcaatgg gcttcaacga aaagcgcggt gacagtgttt
     7141 ttgttggaaa catgcctttt gaagctgcag ttgaagacga taaggttaat gaaattgagg
     7201 ccactagagt caggaacaag tttatactgg atattgtgcg ttatgtggct cttggtttgg
     7261 ccattttgtc tctcattatg tttgtaatcc gtccgatggt tcagcggctc agttccaaac
     7321 cggaagacct tgatctcctc atgggaatgc cggcaacgat tggtgagctt gaagaagagg
     7381 agttagaagt cccaactgag cgagaatctg gaattcctcc gagggaaaaa attctcgaaa
     7441 ttgccaagca ggatccgctc aagacatccg cactcatcca tacctggcta cgcgaacgat
     7501 gataaagtta attcagtatt cagattttgt ggtgcagtta aaaaaacttt cttccacttt
     7561 tcactaaatc ctgaatacca gatccttaat cctaaataat ttaacattgt tctaaccatg
     7621 ttagaagata gtgagttcaa accttttaac ttgaccgatt tcacggttgt tgaaactctg
     7681 cctgaagatt ctgaaagttt atctgcaact caggaagatg gtcaagatgg agccgaagtt
     7741 gaagagtcag aaatttcagt tcgtctcgct tctttcaaac ccttaattat tgatgaacaa
     7801 ggaggctctg aggaatttga tgcaaatggt tcaaccgatt tggcagacag aaggactgat
     7861 gcagaaaaaa ttcatttgtc tcaggatttt agaacagctg atttttttca ggaaaacagt
     7921 ctcctgacaa atgcagaaaa ttttgctgaa acaattagag atggagcaaa gctttacaaa
     7981 acacagttgc taacaaaaat cgaagaacag gcaactgata ccaaacgaat tcacaaaaaa
     8041 actgttgctg aaaatcagga ggcagaagag gagagaaaaa agctgttgtc tgctacagaa
     8101 gaaaaagtcg atgaaataaa aaatgaagcc ttcaatgaag gttttgaggc agggcgtctt
     8161 aagggaatgc agaagcgcta tgatgaggcg gggcccttgg ttttgcaggt aaattcagta
     8221 atggaacaat tgaattcctt acggcaagca gtacggtttc aggcggaaaa ggaattagtg
     8281 gaattggcgt tgcaaatagc aaaaaaagta gttgccgaag aaattaagct gccaaatgat
     8341 gttataaaaa atatcgtaaa ggctgcactt catgaaacag aagtgcaagg taaaatctat
     8401 ctgtacttgc atccggatga ctacgaattc ctgctaaaga gcaaggcaga tttagaacgt
     8461 tatttaaatg aagagcaaac acttgttctt cgacagaatc cggaattgaa acccggttct
     8521 atttatgtgg aatctgatga agaaataatc agtcgttcaa tagaggatca gtttgataaa
     8581 ctggaagaaa ctttgacaga acaaatagaa aatcgccatg ctcaattatc agaagtcgat
     8641 ctcgatgcac acgatttttc aatacggact tcctcggacg atgctgcaaa tgcagattct
     8701 gctgctgttc atttacagga cgtgggagaa gtggattctc cggcagaaac agatgagaag
     8761 gcagaactta aaaaagcgga tcctgaaaaa agtacagtaa aagatacaga ggaaattgct
     8821 cagaaaacta tgcccgttga ctcaagtgaa gttgacgata atgagaaacc cgtgacacag
     8881 tctggatcag ttgatttacg tgaaatggat gaaacagacc tttccgaaga ggctgcagat
     8941 ccagctttgg aaacagaatc aattgaaaca gaagaacaag aacagcaaac aaaacctgta
     9001 gaaaataagg agccggaact ggaaactgaa gccgccaaaa ctgaggagtc agtaccggaa
     9061 acagagactg atgagtctgc agagctagat ccacccaaat gacaagatta tttcagaaac
     9121 aaaaaatatc aagattccca aattattttt tgcagcccct aatacatgaa cacaaaattc
     9181 caaaacctga acttttttaa ttttcatgct ttcagaaact cctgcatccg aattaccggt
     9241 ttattccaac cttgggacat acatcagtga aatagagaaa ttccctgctg tgcgaaagga
     9301 aggacgcgta atgcaggtaa ttgggcacat ggtagaagct acaaatccag gttgttctgt
     9361 aggaggtatg tgtaaaattt ataatccggc aacaaaaagc tccgttactg cagaggtcgt
     9421 cggattccgg aaagaccgga tgctcattat gcccctgcat aacacacacg ggatcggccc
     9481 aaagtgccgg attatcccgg aagatagacc ggctatggta ccagtaggtg acggattgct
     9541 gggaagagtg atagatcctt tgatgcgtcc gatggacggg ctgggtgaac tgccaacttc
     9601 aactgaggta cctttatatc cggaagttgt aaatccaatg aatcgccaac gtatcaaaaa
     9661 acctttggat gttggagtaa gatctataaa tgcatgcctg acctgtggca gaggacagcg
     9721 tatcggtata atggctggat ccggtgtcgg caaatcagtt ttgcttggaa tgatggcacg
     9781 ttacacagat gcggacataa acgtcatttc acttgtagga gagcggggca aggaggtcag
     9841 ggaatttatt gaagataatt tgggcgaaga aggaatgcgc cgttcggttg tgattgtggc
     9901 tacatcagat caaccgccgc tactaagaat gcgtggagca tacattgcca cctcaattgc
     9961 cgaacacttc cgcaacgaag gccaggatgt actactttct atggattctc tcacacgttt
    10021 tgctatggcg cagcgtgaaa ttgggttggc tgcaggcgaa cctcctacca caaaaggtta
    10081 tcccccctct gtatttgctt tgttagctgg actgcttgaa cgcacgggaa ctcatgacgg
    10141 acctggttcg attacaggat tttacactgt tctcgttgaa ggcgacgatg caaatgatcc
    10201 cattgccgat gcaattcgtg caatagttga tgggcatatc tatttaagcc gggaaattgc
    10261 ggaaaagggt acgtatcccg cggttgactt actatcttcc acaagccggg ttatggtgga
    10321 tgtcgtcggt gaaaaacacc ttaaactcaa tcaaatcatg cgtcgcaccc tggctaccta
    10381 cagggaggct gaagacctga taaatatcgg agcttatgtc caggggagca atcctgaaat
    10441 agactatgca atcacaaaaa aaccgctaat tcaggaattc attcagcagg gtatgaacga
    10501 acatactgat ttgaaagagt gtgaagagca catccttcaa attttcggtg accgattgga
    10561 agaagacacg actccggaag aaaattctgc ttaaccagac ataatcaata acaacactgg
    10621 atcttccaat ttttcatttt c
//
LOCUS       DTVD01000064            2683 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15723_length_2683_cov_539.943683, whole genome
            shotgun sequence.
ACCESSION   DTVD01000064 DTVD01000000
VERSION     DTVD01000064.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15723_length_2683_cov_539.943683"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            153..761
                     /locus_tag="EYO68_02395"
     CDS             153..761
                     /locus_tag="EYO68_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIC06684.1"
                     /translation="MKETEIILIRHGETEWNSQQRMQGHSNSDLSSVGQAQIQALGQW
                     MKNVPFDHIYSSDSLRAKQTAEAITQFSGHELKIDLRLREKNLGVFEGLTSEEARERH
                     PEVFRLFKTAGSKYVIDEGESTQQLQDRALEIVDEIRIKHPEEHVLLVTHGGFIRVVM
                     KHSLGLSLETPTRFLIRNTGVFRLVWEDKWIVSQMGGVSHLE"
     gene            874..1638
                     /locus_tag="EYO68_02400"
     CDS             874..1638
                     /locus_tag="EYO68_02400"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC06685.1"
                     /translation="MKILQRSRPERLIWENENSGVTVQVLEKEGRRELRFGNHIMQSV
                     FSTVNPDHLVLPYTRFMLLGLLFCPEPKSVLHIGLGGGSIVRWMYREFPTIQQTIIEI
                     NPAVIEAARRFFEFPLDKRLSVMQADATQIITKLKLKYDLIFLDAYGEYGPPEEVTRT
                     DFLQNLSNCLNTGGWLVGNLWTITGNFMERREQWKSTFTQLFQARANQKGNIILFASK
                     IQQLREKQQFNETAKMLNKRHPLDFRKMLSELKTVI"
     gene            complement(1732..>2683)
                     /locus_tag="EYO68_02405"
     CDS             complement(1732..>2683)
                     /locus_tag="EYO68_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06686.1"
                     /translation="EKDDDDKNNGESENISPASPTACKSTVKVVGNVGDTAANMITTA
                     VAKSANYSKGLTIFGINILATSEVPDEKLLHAANITAELIDNNEDGTPDNTCVTAILS
                     KLGGYVSMYNKAEGNSVEINQDPLDEVGAGGSGLGAYETLNNYADGKSHDASIEEIFH
                     LITQRGYSRVYPKVFGESNTSTSSLAKAMDTARGGEQRCAKEECNWNYNNTSCPIWSD
                     LVDSGDAWYFYADPTADYATMMTEYIYWSVSSNFGMHDSSAGRKKAKTEWCPNTKEIL
                     KAQDPAVYNLIKDPKYAFPTVLPNADYTYYTFKTSDIKSF"
BASE COUNT          765 a          525 c          576 g          817 t
ORIGIN      
        1 gttaaagtac tcttcttttt atcccgttgc tgtttttatc tctccagttt aataaatgaa
       61 aagttcggaa gtttccacac ttaaaacacc atcaggctac gttggatact tgatgaacgg
      121 attattcacg aaaaaaatca aagtagtaat ttatgaaaga aacggaaatc attttgattc
      181 gccacggtga gacagagtgg aactcacagc agcgcatgca gggacattcc aacagtgatt
      241 tgtcatctgt aggtcaagca caaattcagg cacttgggca gtggatgaaa aatgtacctt
      301 tcgaccatat atactccagc gattccctgc gtgcaaaaca aactgctgag gcaattactc
      361 agttttcagg acatgagctg aaaattgatc tgcgtctgcg tgagaaaaat cttggcgttt
      421 ttgaaggatt aacatctgag gaagccaggg agcgtcatcc tgaagtcttc cgtcttttca
      481 aaacagcagg ctcaaaatat gtaattgatg aaggtgaaag tacacagcag ctgcaggatc
      541 gtgccttgga gattgtagat gaaatccgga ttaaacatcc ggaagaacat gtattgctgg
      601 tgacacatgg tggtttcatt agggttgtaa tgaagcattc actggggctc tctctggaaa
      661 cacccacacg ctttttaatc aggaatactg gtgtattccg tttagtatgg gaagataaat
      721 ggattgtttc acaaatgggc ggagtttcac atttggaata aatgaaggaa tcggttttgt
      781 caggctggtc tgaaaccgtc actaaagtac atctttgaag cctttagcct tttattttta
      841 acctttaatc tagaatctta atttttgaat ctattgaaaa tcctgcaaag aagcaggccg
      901 gaacgtttga tctgggaaaa tgaaaattct ggtgttactg ttcaggtctt ggaaaaagag
      961 ggtagacgtg aattgcgatt tggcaaccat atcatgcaga gcgttttttc cactgtcaat
     1021 ccggatcatc ttgtcttgcc ctacacacgc ttcatgctgc tgggactgct tttttgtccg
     1081 gaaccaaagt cagttctgca tatcggtctt ggaggcggca gcattgtaag atggatgtat
     1141 cgtgaattcc ctacaattca gcaaaccatc attgaaataa atcctgcagt aatcgaggcg
     1201 gcacggcgct tttttgagtt tccacttgac aagcggttga gtgtgatgca ggcggacgca
     1261 acacaaatta ttacaaaact taaattgaaa tatgatctca tatttttgga tgcatacggt
     1321 gaatatgggc ctccggaaga ggtgacccgt acagattttt tgcaaaacct tagtaactgt
     1381 ctgaataccg gcggctggct tgttggaaat ttgtggacaa taaccggaaa ttttatggag
     1441 cgtcgtgagc aatggaaatc aacatttact cagctttttc aggcacgtgc taatcagaag
     1501 ggaaatataa tactttttgc cagcaaaatt cagcaacttc gggaaaagca gcaatttaat
     1561 gaaactgcaa agatgctaaa caaacgtcac ccacttgatt ttcgtaaaat gctgagtgaa
     1621 ttaaaaactg taatttaaaa ttggttttgt tgaacaaact gtcctcaact acattattcc
     1681 aagtagaaat tcaagtagga catgctagaa ctaattcaag tatacgattt attagaaaga
     1741 tttaatatct gaagttttga aagtgtaata cgtatagtcc gcatttggta aaacggtggg
     1801 gaatgcgtat ttcggatcct tgattaagtt gtaaactgca gggtcctgag cttttaatat
     1861 ttctttagta tttggacacc attctgtttt tgcttttttg cgccctgcgg aagagtcatg
     1921 catgccaaaa ttggaactga cggaccagta gatatattcg gtcatcattg tagcatagtc
     1981 agctgtcggg tcagcataaa agtaccaggc atcgccagaa tccactaaat ccgaccaaat
     2041 gggacaggag gtgttattgt aattccagtt gcattcctct tttgcgcatc tttgttcacc
     2101 gccacgggca gtatccattg cttttgccag ggaggaggtg gaagtattgg attctccaaa
     2161 aacctttgga tagactcttg agtaaccgcg ctgagtaatc agatgaaaaa tttcttcaat
     2221 acttgcgtca tggctcttac catccgcata attgttcagc gtctcgtagg cacctaagcc
     2281 ggaaccgcct gcccctactt catccagagg gtcttggttt atctcaactg aatttccttc
     2341 agctttatta tacatgctca cgtaaccacc cagtttactc agtattgcag tgacacaggt
     2401 attgtccgga gtaccgtctt cattgttgtc aatcagctct gcagtaatgt ttgctgcgtg
     2461 cagcagcttt tcatctggga cttcagaagt tgccaatata tttattccaa aaatagttaa
     2521 acctttagaa taattggcgc tttttgctac agcagtagtt atcatatttg ctgctgtatc
     2581 acccacattt ccaactactt taactgtact tttgcaggct gttggtgaag caggtgaaat
     2641 gttttcactt tcaccattat ttttgtcatc gtcatccttt tca
//
LOCUS       DTVD01000065            2668 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15854_length_2668_cov_408.456563, whole genome
            shotgun sequence.
ACCESSION   DTVD01000065 DTVD01000000
VERSION     DTVD01000065.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15854_length_2668_cov_408.456563"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2663
                     /gene="polA"
                     /locus_tag="EYO68_02410"
     CDS             <1..2663
                     /gene="polA"
                     /locus_tag="EYO68_02410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIC06687.1"
                     /translation="FLMQLVKIIEEERPDYLAVTSDSSEPTFRHKRFPDYKATREKMP
                     EDLVEQLPYLPRLVEALNLPYLILPGFEADDIVGTLMRLCREADIEGVMVTSDKDYMQ
                     LVTDKIVMLNHRNERIGIPGVREKFGCAPEQVIEMLGLMGDSSDNIPGVRGVGEKTAM
                     KLIAEYGNIQSVYENLENINGKSLKEKLTADHENALLSRELATIDCHVPISVTLEDVH
                     LGETDLYDNPKFYALLEELEFRTLLNRLHKKRAAGSKKQEEVNLREDENNKEKGEFVA
                     ETEGEWNVLEISCYKIETAEQFRAYMSEVPEGKCLALALNTQGDHQLEFRLLGLALCA
                     NQDRGIYLDLRNADSESTEMFAEVKKILESEAFPKIFHGLKKAVQLFTNLGIELKGIG
                     SDVMLAAHITDPLGRRYDLDYLLGRKLNLRRKPIKSAEKSSPENQLSMFEEADKEEYI
                     LHCENSSIILRLHGQLGAQLKQTGMEGAFRNIEIPLAGTLAKLELNGVRLDVDGLAKI
                     ALEFDGRLVELRQKIFDLAGEEFNPNSIVELQKIIYEKLRLHERFKVKPKKIKLGNQM
                     STDEETLEKLAADELPRTILAYRTLNKLKNTYVDQLPTYVHAESGRIHSTFRQTGTAT
                     GRLSSENPNLQNIPVRTSEGRRIRRAFIASGADKILISADYSQIELRVVAHYSKDPTF
                     MDAYRRNLDIHSLTASAIFSVPEQDVTREMRSRAKEVNFGLIYRMGPERLSVVTKTSK
                     VEAKEFIERYFQKYSTIHALQERFLGQARKEGFSETLFGRRRYLPEINAKGLLKRMAE
                     GAAINTPIQGSAAEIIKLAMIAVDQRIREKQMQSRMILTVHDELVFDTLIEEEEELCE
                     MVKHTMENVVGLEVPLLVEIGKGKTWIDAH"
BASE COUNT          845 a          487 c          631 g          705 t
ORIGIN      
        1 cttttttaat gcaattggtc aagatcattg aagaagaaag accggattat ttagcagtta
       61 cttctgattc atcggagccc acttttcgcc acaaacgatt tccggattac aaagcaacac
      121 gagaaaaaat gccggaagat ctggttgagc agcttccata tttaccaagg ctggttgagg
      181 cacttaattt gccatatttg attttgccag gatttgaagc agatgatatt gtcggtaccc
      241 tgatgaggct ttgtcgggaa gctgatatag aaggtgtgat ggtgaccagt gacaaagatt
      301 acatgcagtt ggtaacagat aaaatagtaa tgctgaatca ccgtaacgag cggataggga
      361 ttccgggagt tcgagaaaaa tttggttgtg ctccggagca ggtgattgaa atgctggggc
      421 tgatgggtga ttcttcggat aatatcccgg gtgtgcgtgg tgttggagaa aaaactgcca
      481 tgaagttgat tgcagaatat ggaaacattc aatcggttta tgaaaatttg gaaaacataa
      541 atggaaaaag tctcaaagaa aaattgactg cagatcatga aaatgcactc ttgtccagag
      601 agcttgctac cattgattgt catgtgccta tttcggtcac gcttgaggat gttcatttgg
      661 gtgaaactga tttatatgat aatccgaaat tttacgcatt actggaagaa ttggaattca
      721 gaactctgct aaatcgtctg cataaaaaaa gggcagcagg aagcaaaaaa caggaagagg
      781 ttaacctgcg tgaggatgaa aacaataaag aaaaaggtga atttgtagcg gaaacagaag
      841 gtgaatggaa tgtgcttgaa atcagttgtt ataaaattga aactgctgaa caattccggg
      901 cttacatgtc agaggttccg gaagggaaat gcctggccct tgccctgaat acacaaggcg
      961 atcatcaact tgaatttcgg cttttggggc tcgctctttg tgcgaatcag gatcgaggca
     1021 tttatcttga tttgagaaat gcagattcag agtcaactga gatgtttgca gaagttaaaa
     1081 aaatactgga gtctgaggct ttcccaaaga tctttcatgg tttgaaaaaa gccgtgcaac
     1141 tttttacaaa cctcggcatc gagttaaagg gaattggatc tgatgtcatg cttgccgccc
     1201 acatcacaga tccattggga agacgctatg acctggatta tctgctgggc cgtaaattga
     1261 atctgcgtcg aaaaccgata aaatcagctg aaaagtcatc cccagaaaat cagctttcga
     1321 tgtttgagga agccgataaa gaagaataca ttctgcattg tgaaaattcc tcaatcatat
     1381 tacgtttgca cggacagctt ggtgcccaac taaaacaaac aggaatggag ggagctttcc
     1441 ggaatattga aatacctctt gcaggaactc ttgcaaagct ggaactgaat ggtgtaaggc
     1501 ttgatgttga tggtttggca aaaattgcac ttgaatttga tggccgtttg gttgagcttc
     1561 gtcaaaaaat ttttgatttg gccggagaag aatttaatcc taactccatt gtagaactgc
     1621 aaaaaattat ttacgagaaa ctgcgtctgc atgaacgttt taaggtaaaa cccaaaaaaa
     1681 taaagctggg caaccaaatg tctacagacg aggaaaccct ggaaaaatta gctgcagacg
     1741 aattgccccg cacaatttta gcttaccgca ctctcaacaa acttaaaaac acatatgtag
     1801 atcaactacc aacctatgtg catgctgaaa gcggccgaat ccactcaact tttaggcaga
     1861 ctgggactgc aacaggacgt ctttcatcgg aaaatccaaa tctgcaaaat attcctgtcc
     1921 gtaccagtga ggggcgtcgt attcgtcgtg cctttatagc gtcgggagca gataagattc
     1981 taatttcagc agactacagc caaatcgaat tgcgcgtagt tgctcattat tcaaaggatc
     2041 caacatttat ggatgcatat cgacgcaacc ttgatattca ttcgttgacg gcttcagcta
     2101 tcttcagtgt gccggaacaa gacgtgacgc gagaaatgcg ttctcgagcg aaagaagtta
     2161 attttggcct gatttacaga atggggccgg agcggctttc agttgtaaca aaaacttcca
     2221 aagttgaagc aaaagaattt atcgaacgtt atttccagaa atacagtacc attcatgccc
     2281 tccaggaaag atttttggga caagcacgta aagagggttt ttcagaaaca ttattcggga
     2341 gaagacgcta ccttcctgag ataaacgcta aaggtctctt gaaaaggatg gcggagggag
     2401 ccgcaatcaa cactcccatc cagggttctg ctgcagaaat cattaagtta gccatgattg
     2461 ctgttgatca gcgtattcgg gaaaaacaaa tgcagtccag aatgattctg acagtccacg
     2521 atgaactggt ctttgataca ttaatagaag aggaagaaga actttgtgaa atggttaagc
     2581 atacaatgga aaatgtagtt ggacttgagg tgccgcttct ggtggaaatt ggtaaaggta
     2641 aaacatggat tgatgcacat tgatcttt
//
LOCUS       DTVD01000066            2659 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_15936_length_2659_cov_221.522657, whole genome
            shotgun sequence.
ACCESSION   DTVD01000066 DTVD01000000
VERSION     DTVD01000066.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15936_length_2659_cov_221.522657"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(109..324)
                     /locus_tag="EYO68_02415"
     CDS             complement(109..324)
                     /locus_tag="EYO68_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06688.1"
                     /translation="MNYQIEPLQTEDWPQVRSIYAESISTGVSTFDTKPPNWKDWDSS
                     RLPSCRFVARDGKYIYGWVSLSPASST"
     gene            complement(860..1261)
                     /locus_tag="EYO68_02420"
     CDS             complement(860..1261)
                     /locus_tag="EYO68_02420"
                     /inference="COORDINATES: protein motif:HMM:PF04828.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIC06689.1"
                     /translation="MRTGECGCGNIKIRTNGEPEIRLICHCKSCQKRTGSDYSHNVYF
                     FHEQVEIIGEVKNYTRTAQEDRFLTNYFCPECGMTLYWHMDVLPKITGIAISVFDVDD
                     FDMPEESIWEKFKRPWVEFSLQLKHLHGQSE"
     gene            complement(1353..1724)
                     /locus_tag="EYO68_02425"
     CDS             complement(1353..1724)
                     /locus_tag="EYO68_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06690.1"
                     /translation="MAEVLWHELVTQKDSTDARDSSGNTFEYLASIKRVNVKTNKGCS
                     FQMDRMTRNNLHRVTRNTAFYFGVFSDHLVLAKIWKVEILVVLAEVERQLDRCKNDIA
                     HVNFLLRWLEDQGERVFATNF"
     gene            2086..2343
                     /locus_tag="EYO68_02430"
     CDS             2086..2343
                     /locus_tag="EYO68_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HIC06691.1"
                     /translation="MIHRHHLDQSAINRAIKQAVRQTEITKKVSAHTFRHSFTKHLLQ
                     RGTDIRTIQALLGHKDLETTMIYTHVLKQGVQGVVSPLEDL"
BASE COUNT          776 a          599 c          508 g          776 t
ORIGIN      
        1 ttcattttca gatgcagcaa aaagagcctt gagcaaagct gtacctattc cttgtctttg
       61 ccctgcctta cctacgtgta cgctgacctc agcgactcca acgtaacctc aggtgctgga
      121 ggcaggactc aaactcaccc agccataaat atattttcca tcccgtgcga caaaacgaca
      181 ggatggtaag cgactggaat cccagtcttt ccagtttgga ggtttggtgt caaaggtgga
      241 aactccggta ctgatacttt ccgcatatat agagcggacc tgcggccagt cttcagtttg
      301 caatggttca atttgatagt tcatatttga ttctttttta ggtttctaac gcctcaccca
      361 ctgaacttgt ttagttagcg actgttttta aacaaaaaag taacagcctt accttgtccg
      421 aatgcagcac cttgttatag gtaatttccc ttgtatttga cttgaacaaa gtcgttctga
      481 aagaccattg cttcagcttc ttcaagccta tggcaataat tcacacagca gggacgggcg
      541 cccagtttac tttgtacttg aaggaaatgt caatccacgg gatgcagagg aaagaggcct
      601 tagtttagtg taccctaatc cccagccatc aaatttctcg tacgctagga aaggcacgcc
      661 acactgaggg cacgctcggg cgtactctga cacttagttt tggcaatctc tgcagcactt
      721 cattattctc ctcatctaac ttattatttc caggcaattt gccagaataa cgcaaagttg
      781 agacgcaggc ggcacataac gataaacata ggaaaaagga aaaagtgacg agtaattaat
      841 tatatattgc taaaagccct cactcacttt gaccatgtaa gtgcttgagc tgtaagctga
      901 attctaccca agggcgttta aacttctccc atatagattc ttcaggcata tcaaaatcat
      961 caacgtcaaa aacactgata gcgatgccag ttattttagg aaggacatcc atatgccaat
     1021 aaagtgtcat accacattcg ggacaaaaat agtttgtcaa aaacctgtct tcctgtgccg
     1081 ttcgcgtata gttttttact tcgccaataa tttctacttg ctcatgaaaa aaataaacat
     1141 tgtgagaata atctgacccg gtacgttttt gacaagattt acaatggcat atgagcctga
     1201 tttctggctc accatttgtc ctaatcttta tgtttccgca cccacattca ccagttcgca
     1261 tcttgtaaat cttactttct agggtttttg aaaaataacg ctaagctaac cgtccacacg
     1321 gcgtagccgc ggggtacgta ttgagtgatt tattagaagt ttgtggcaaa cactcgttcc
     1381 ccctgatcct ccagccatcg caaaagaaaa ttcacgtgag caatgtcatt cttgcaacgg
     1441 tcaagctgtc tctcaacctc agcaagcaca acaaggatct ctaccttcca gatttttgcc
     1501 aagaccaaat ggtccgaaaa gaccccaaaa taaaatgcgg tatttcgtgt tacgcgatgc
     1561 agattatttc tggtcatacg atccatctga aaactacaac ccttattcgt ttttacgttt
     1621 actcgcttga tgctggccaa atactcaaaa gtgttgccac ttgagtctct tgcgtccgtc
     1681 gaatcctttt gggtcacaag ctcatgccac aagacttcgg ccataatcag ttccttaatt
     1741 acacctggtt gcgtggatta gagattcatc gctcatgagt aacttctaac ttattattat
     1801 caagcaactt accctgtaac cactatatta tccagtaaat cggttaagat acctgataat
     1861 ccggaaataa ccggaaattc aggcattata cctgaaaacc cttgtattat ccgttaaatc
     1921 gggtaaaatt agccacaaat ggaatattcc gacaagttag cttgattagt tgagcttgaa
     1981 cagctaatca agtatatgta taggttaatt ccgattagtc gcaagtctaa aacaccacaa
     2041 tatgtatttc ctgttctggg tctctcaaaa gactctcgta gttgaataat acaccgtcac
     2101 catctggatc agtcggctat caacagagca ataaagcagg ctgtccgtca aacagaaatt
     2161 accaaaaaag tgagtgcgca tacattccgg catagtttca ctaaacattt gctacagcgg
     2221 ggaacagata ttcgcaccat tcaggccttg ctgggtcaca aagatttgga aacaaccatg
     2281 atatatactc atgtgttgaa gcagggcgta caaggtgttg ttagccctct tgaagattta
     2341 tgattagttg ccatttcaag gaaaaatgta gggtgttttt cttggctttg cactggatct
     2401 ataaaaaatt tctttattat tctttggttt ttagattaaa tcagaattaa actctgtcga
     2461 tatttattcc tgacaaaata tatttagcaa atattcctga tgtttcagta ccacccagag
     2521 agaattatta ttgataaagc agttgccgca gagcctctga ctgagcaaat ttgcgggcgc
     2581 tttccagagg ttccaacaca gttagtggaa aatttcgcct ggcatcagga cgagtccgga
     2641 acagatcctc tgcgcaatc
//
LOCUS       DTVD01000067           10488 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1600_length_10488_cov_405.491613, whole genome
            shotgun sequence.
ACCESSION   DTVD01000067 DTVD01000000
VERSION     DTVD01000067.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_10488_cov_405.491613"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(179..478)
                     /gene="atpE"
                     /locus_tag="EYO68_02435"
     CDS             complement(179..478)
                     /gene="atpE"
                     /locus_tag="EYO68_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIC06692.1"
                     /translation="MKKLLLLTILLSASFPLYAAEMGEMATFGIALGAGLAIGLAALG
                     GGIGQGIAMSGALEGIARNPNAYDKIFTPMIIGLALIESLVIYALVIAFMLQGRI"
     gene            complement(513..1292)
                     /gene="atpB"
                     /locus_tag="EYO68_02440"
     CDS             complement(513..1292)
                     /gene="atpB"
                     /locus_tag="EYO68_02440"
                     /inference="COORDINATES: protein motif:HMM:PF00119.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIC06693.1"
                     /translation="MNKRLALVSVFLVTATTVFAAEGAFTWSHFLIGWLEQPLVELGI
                     DPVPMLDLLIVAFLLIIFAYFAGKPYRNTDMVEPSGRADFLNFAEVIVSGILNFLSGT
                     IRHGVGARPILPLLGTYGLFILLLNLAGLVPGFNPPTDQFNVTIAFAVIIFLMTHFLG
                     IRQHGASYIKQFLGPMPVLAPLMFPVEVVSHCVRPLSLSIRLFGNMTGDHKVVMIFTS
                     ILAVGLPIPFMGLGILVSVLQAFVFVLLSAIYFELAMSEAH"
     gene            complement(1309..1746)
                     /locus_tag="EYO68_02445"
     CDS             complement(1309..1746)
                     /locus_tag="EYO68_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06694.1"
                     /translation="MKTDTKPDTAASEVPENLNSGKKSSHIKQADFFRNLLLGISIIL
                     VAGTAIFQLDLVPSVMLGCAVVGFNYYWTVKFVQKLLLERKLFALDLLFILTKFAISV
                     IVLFGALTYLKLPPMGLLIGLSNVALAAIMYSFVRVMNPHKFA"
     gene            complement(1836..2048)
                     /locus_tag="EYO68_02450"
     CDS             complement(1836..2048)
                     /locus_tag="EYO68_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIC06695.1"
                     /translation="MYSSMGLELGLSVVVGFLIGSWLDEWLDTKPWFLLVFGVSGIIA
                     GYRSIFRLVKRVQADSEQEHDSPSSS"
     gene            2279..3358
                     /gene="aroC"
                     /locus_tag="EYO68_02455"
     CDS             2279..3358
                     /gene="aroC"
                     /locus_tag="EYO68_02455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIC06696.1"
                     /translation="MGDSFGHLFRITTWGESHGGGVGVVIDGCPSGLQLSEDDLQYEL
                     DRRRPGQSKITTQRKESDTARILSGVFEGRTTGTAISVMVLNEDAKSHAYEHLKDLYR
                     PSHADFTFDKKYGFRDWMGGGRSSARETVGRVAAGAIAKKLLQQWNNIQTIAWVEQIH
                     EIKSSVDKESISEQKIEASIVRCPDPEASEAMIARIHEVRKSGDSVGGIIRAVVRNVP
                     AGLGEPVFDKLTADLAKALMSLPATRGVEFGLGFYSVKLKGSEHNDAFVMRDGTIRTN
                     TNRSGGIQGGISNGEEIDLRVSFKPTATINFEQDTVTREGEAVKLKAKGRHDPCVLPR
                     AVPMVEAMINLVLTDHYLRNRQSRL"
     gene            3673..4629
                     /locus_tag="EYO68_02460"
     CDS             3673..4629
                     /locus_tag="EYO68_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIC06697.1"
                     /translation="MPYIDVFNGDADGICALHQLRLHNPQKSSLVTGVKRDNLLLKRI
                     IATRDSTLTVLDISSHANRDSLLQLLKQGNTVHYFDHHFAGEMPESPLFHPHIDTSPD
                     VCSSILVDRFLSGKYRLWAIVASFGDNLHVSAHELAGSLKLDPKKTEELRELGELINY
                     NAYGETIDDLHFSPEVLFKALQPFSDPFEFYHASGELNQLREGFRNDMRKAESELPVR
                     QTSAGRIYQFQNQAWCRRVSGVYSNQVAREAPDLAHALLVERKDGTFLVGVRAPISKP
                     QGAEKLCIKFSGGGRAAAAGINNLAPEEVDRFFDAFDLQFTI"
     gene            complement(4668..5333)
                     /locus_tag="EYO68_02465"
     CDS             complement(4668..5333)
                     /locus_tag="EYO68_02465"
                     /inference="COORDINATES: protein motif:HMM:PF04028.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HIC06698.1"
                     /translation="MKEAKSSLKRRFQRWLITKTLMLLIRITGNTTRIKSNNQQILKT
                     ALQEYGSVIVATWHQNIYFSVWLLRNQELTALISSSDDGEVIYDVFAHFGYQAVRGST
                     TRGGIPALKQLIKLLKDKTSVAITPDGPLGPPEKIQSGVVLLAKYSGVPIIPWHYEAK
                     HLWKLKSWDRHKIPKPFTTIVESFGEPFHVPKELLPEEVPVICGKLEAILQELALKTG
                     EEI"
     gene            5692..7122
                     /locus_tag="EYO68_02470"
     CDS             5692..7122
                     /locus_tag="EYO68_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIC06699.1"
                     /translation="MGTLLLVTSIALLFPIITSLIYQENDFNALLISAVAAFGMGLPL
                     WWFSPKDYELSFRDAVLIAVFGWVIISVVSALPFVIHGSIPSFTDAFFEMMSGYTTTG
                     ATILTDIESLPHGLLFWRSETHLLGGMGFLTLTLIFLPHGMAGVRIFRAESSPGQVIT
                     KEKFLPRNRDAMIWLWSIYLGLNMLQTLMLWGGGMSLFDSLCHAFGTVATAGFSPKNT
                     SLGYYGNAYFDWVVTIFMFLGGMTFMLFYHMLKGEWNLVRINTELRWYAIIVGFFCGI
                     STLILWMNGTYDRFFDALRHASFQIISLLTTTGFTTADYELWPQSAQMLLYVVCFIGA
                     CAGSTTSGIKIVHFVIIWKYMVTTIKKMFFQPRTILPILLNQRAIDPFAVNLSICYFI
                     ANIFLVLGGSCVMVLLDSMDFQTGMSSVIAAVMNVGPGFGDVGPSENYAHISAAGKWF
                     LAWLMLVGRLEMFSVLVLLFPSFWKK"
     gene            complement(7252..9231)
                     /locus_tag="EYO68_02475"
     CDS             complement(7252..9231)
                     /locus_tag="EYO68_02475"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02824.19,HMM:PF13328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIC06700.1"
                     /translation="MGNLSGTEISEDILSPTITIGHSADINSSVVPEAFLTSPEATSL
                     FENWYSKQSNPDAAAALLKNLKQLSLSPATLQAALLFLQYQAADDLPKEFEYKTSIDS
                     KGSFSDALPLYHRMLKVQQFPVRPKNSEQAELTLRMLLTVLGDVQLLTVFLVLQLQQL
                     EQLDSLSPKERDETAWTALHVHAPLAGRLGIFWIKAEMEDRAFRQLEYENYQNLKKKI
                     ARKRSDRSESVEIISEKIRHILTEAGITHEVQGRYKRFYSIFQKLEKVENNFDRIQDL
                     IGFRVLVNGIDECYAALSFIHENWTPIKNRFKDYIANPKSNGYQSLHTTVTDSQNGPL
                     RYLRPIEIQIRTHKMHRMAEYGVAAHWLYKEKRQPSKGKKAEQIEVSLAEQSKAGRHN
                     ETIPLIDLFSDNIYVMTPSRDIRELPQTATPVDFAYAIHTEVGNRMTGAKTNGNITRL
                     DSSLQSGDTVEILTSPRQEPRKEWLDFVKTRHARNKIKHALHERSREGRRKEGLEMLE
                     REFKNHSLNLNRLIREGRMEKESRLHKNQEFEHILFSIGDGSIRSEEVRRWFADDPSS
                     VTASTPESPAQPETKAKTRKKRIAESPSTNKILVDGMDNVMTRIAKCCSPDKSHSIQG
                     YLTLERVITIHKYGCAYLQKLTPERMVKVRWGNRK"
     gene            complement(9255..9854)
                     /locus_tag="EYO68_02480"
     CDS             complement(9255..9854)
                     /locus_tag="EYO68_02480"
                     /inference="COORDINATES: protein motif:HMM:PF00293.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIC06701.1"
                     /translation="MSEIQTNNPPNAGTARVLDEQLLFRGNWAEIVEFSYEDDKKQVR
                     KWEGLHRKHRAEAVIIVARMQPSGRFIIIRQFRPPTNSYILEFPAGLVDPGESQETTA
                     IRELFEETGYVGTVENISPRLFSSPGILSETVSFVQVQVDENIAENLQPKPENEPGEF
                     ITVFVKSPDEIAEFFKQEKKQGVQFDVKLYTYFMVQGLL"
     gene            10058..>10488
                     /locus_tag="EYO68_02485"
     CDS             10058..>10488
                     /locus_tag="EYO68_02485"
                     /inference="COORDINATES: protein motif:HMM:PF02590.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIC06702.1"
                     /translation="MRSIRLIWIGKSQEPFVQDGIKVYLKKLSHYIKVDFEEIKPVKY
                     NSGTTDQWRRQETEKLLKRLDSSELNIFLDESGKRETSNSLARWFEKQIPLGWRRVNF
                     FIGGAYGFDKTMLPSGVKLLRLSDMTFTHQMVRLILLEQLYR"
BASE COUNT         2934 a         2171 c         2232 g         3151 t
ORIGIN      
        1 cagacaatgc ttcaggaaat gaatttttgc agtccgatgg cgtattgatt ttagataact
       61 gaaagagtta agcaaaatga tctattattt ctgcctggct tttcacatca aattataaag
      121 gaatcaaatc aaaggtggga gggttgcggc ccgttgagag ccgcagaaaa tgagagcttc
      181 agattctacc ctgtaacatg aacgcgatca caagtgcata aattacgagt gactcgatca
      241 gtgccagacc aataatcatt ggtgtaaaaa ttttgtcgta tgcgttcgga ttcctggcaa
      301 ttccttccaa tgcaccgctc attgcgatac cttgtccgat tcctccacca agagctgcaa
      361 gtccaattgc taaaccggca ccaagagcaa ttccaaatgt ggccatttct cccatttctg
      421 ctgcatacag agggaaactc gctgacagta gtatcgtcaa caataataat tttttcatga
      481 catccttttt gatgttaaat aaatcagcgc aatcagtgag cttcactcat tgcgagttca
      541 aaataaatgg cagacagcaa tacaaagaca aatgcttgta acactgacac caaaattccc
      601 agacccataa acggaatagg cagtccgact gcaagaattg acgtgaaaat catgacaact
      661 ttatgatctc cggtcatgtt cccaaataga cggattgaaa gggaaagcgg gcgcacacaa
      721 tgactgacga cctcgacagg aaacatcagt ggcgcaagta ccggcatggg ccccagaaac
      781 tgttttatgt aactggctcc atgctgacga atgcctaaaa agtgagtcat aagaaaaata
      841 atgactgcaa aggcaattgt aacgttaaac tgatctgtag gcggattgaa accaggtacc
      901 agaccagcaa gattgagaag caaaataaat aaaccgtaag ttcccagtaa tggaagaatg
      961 ggacgtgcac caacaccgtg gcggatggtt ccgctcaaaa aattcagtat tccactgaca
     1021 ataacttctg caaaatttaa aaaatctgca cgtccggaag gctcaaccat atctgtattt
     1081 ctatatggtt ttccggcaaa ataagcgaaa attatcaaca aaaatgccac tatcagcagg
     1141 tccaacattg gaaccgggtc aattccaagt tcaaccagcg gttgttctaa ccagcctatc
     1201 aaaaaatgag accacgtgaa tgcaccctcg gcagcaaaaa cagtggtggc tgttactaaa
     1261 aaaacactga ctagagcaag acgtttgttc attctttaat ttttttatct atgcaaattt
     1321 atgagggttc atcactctaa caaaagagta catgattgca gcaagtgcaa cattggaaag
     1381 tccgatcagt aaccccattg gtggtagttt caagtaagtc aaagctccaa acaaaactat
     1441 tactgaaatc gcgaattttg taagtataaa cagcaaatcc agtgcgaaca attttcgctc
     1501 cagcaacaac ttttggacaa actttacagt ccagtaataa ttaaaaccaa caaccgcaca
     1561 tcctaacatg acagaaggaa caaggtcaag ttgaaaaatt gctgttcctg caactaaaat
     1621 tatgctgatg cccaaaagta gatttcgaaa aaagtcagcc tgtttgatat ggctggattt
     1681 ttttcctgaa tttaagtttt ccggaacttc agaggcagct gtatctggtt tggtatcggt
     1741 tttcatgctt tcggtactat ctctgttatc ttttggaatc ccgttattta gaattgatat
     1801 ttgaattgtc ttcagttgct gtcagcaatt taatttcagg atgaagacgg tgaatcgtgt
     1861 tcctgctcag aatcagcttg aactcttttg accaaacgaa aaatactcct gtaacctgct
     1921 atgatcccgg aaacaccaaa caccagcaaa aaccagggtt ttgtatccag ccactcatcc
     1981 agccaactgc cgatcagaaa acccacaaca acagacaatc ccagttccaa acccatagaa
     2041 ctgtacatta tccattttgg gccagatttg tgtagttgtt tcacttcaca gggaatgtat
     2101 tttacggaaa atatttttta gatcggataa gcaaaaaaca ttagataagt gtagtgtaca
     2161 ctgcctgaaa ttgcaagatt ttgcaaccaa gtctcttgca ccttataggt gaaattgata
     2221 tgctgacatg acttaaacac tggcaaaatc agctatgtaa acttaattga gtttaaaaat
     2281 gggtgacagt ttcggacatt tatttcgtat cactacctgg ggagaatctc atggcggcgg
     2341 agttggagtt gttatcgacg gatgcccatc gggactgcag ttgagtgaag atgatctcca
     2401 atatgagttg gacagaaggc ggcccgggca aagcaaaata actacacaac gaaaggaaag
     2461 cgatactgcc aggattcttt caggagtttt tgaggggcga accaccggta cagcaatttc
     2521 tgtgatggtc ttaaatgaag atgctaagtc acatgcttat gagcatctca aagatttgta
     2581 tcgccccagc cacgcggatt tcactttcga caagaaatat ggtttcaggg actggatggg
     2641 cggcggacgc tcgagtgcac gtgaaaccgt cggaagagtt gcagccggag caatcgctaa
     2701 aaaactgctc cagcaatgga ataatatcca gacaattgcc tgggtagagc aaatccatga
     2761 gataaaatct tcagtggata aagaatccat cagcgaacaa aagattgaag ccagtattgt
     2821 gcgctgtccg gatccggaag cgtcagaagc aatgattgcc cgtattcatg aagtccgtaa
     2881 atccggagat agcgtaggtg gaattattcg ggcagttgtc cggaatgttc cggcaggttt
     2941 gggtgaaccg gtatttgaca aactgactgc agatttggcc aaggcattaa tgtcacttcc
     3001 ggctacacgg ggggttgagt ttggcttggg tttctattca gtgaagctga aaggttcaga
     3061 acataacgat gcgtttgtga tgcgcgatgg aactatccgg accaacacaa accgttccgg
     3121 tggaattcag ggtggaattt caaatggtga agagattgat ttacgggttt cttttaaacc
     3181 aaccgccacg attaacttcg aacaggatac agttactcgt gaaggtgaag ctgttaaact
     3241 gaaagccaaa gggcgtcatg atccatgtgt tttaccaaga gccgttccaa tggtagaagc
     3301 catgattaat ttggttttaa ccgatcacta tttacgcaac cgtcaaagcc gtctttaagg
     3361 aatttgggaa ggaattaatt tattatatgt ttttgggaag ttggttctaa atttgcaaat
     3421 tcataattag aaccaaaaac gaatattttc agctttgaac cagtacaaaa tatgcctccc
     3481 aaaattaacc caatgaaagt ttcatcaagg acaattcgtc ctgcaagtga aaaacgactt
     3541 aattcactga tggcaaactc ggaagctcga tttctttcat cgggttctat tgctgtgttt
     3601 ggggtactcc tttttattgc cttcgtgatt gctcaaataa ttttcattaa ctgaagagca
     3661 agtttagctt cgatgcctta cattgacgtc ttcaacgggg atgccgatgg tatatgcgcc
     3721 ctccatcaat tgcgtcttca caatccccag aaaagttccc ttgtcacagg agtaaaacgt
     3781 gataatctac tgttgaaacg tattattgcc acgcgagaca gtacactcac agttctcgat
     3841 atttcatctc atgccaaccg ggattctctc ctgcagttat tgaaacaggg taatactgtt
     3901 cactattttg atcaccactt tgcaggagaa atgcctgaat ccccactttt tcatccacat
     3961 attgatacga gtcctgatgt ttgtagcagc atactggtag atcgtttttt gtcagggaaa
     4021 tatcgccttt gggccatagt tgcatctttt ggagacaacc ttcatgtatc cgcacatgag
     4081 ttggccggca gtttgaagct ggatccaaaa aaaactgaag aattacgaga actgggagag
     4141 ctgatcaatt acaatgcata tggtgaaacc atagacgatt tgcatttttc acctgaagtt
     4201 ttgtttaaag ctttgcagcc tttttctgat ccgtttgaat tttatcatgc atccggtgag
     4261 ttgaatcagt tgcgggaagg ttttcggaat gacatgcgca aagcagaatc tgaacttcct
     4321 gtaagacaaa cttctgcagg tagaatttat cagtttcaaa atcaagcatg gtgccgtcgt
     4381 gtatccggag tatacagcaa tcaggttgcg cgggaagccc cggatctggc tcatgcacta
     4441 ttggttgaaa gaaaagacgg aacttttctg gttggtgtcc gcgccccaat ttcaaaaccg
     4501 caaggtgcag aaaaattatg cattaaattt tccggagggg gtcgagccgc agcagcagga
     4561 atcaacaatt tggctccgga agaggttgat cgattttttg atgcctttga tcttcaattt
     4621 actatttaat tgaagttgca gtaattttat ggtttgtttt tctgcgttta aatttcttct
     4681 cctgttttga gtgcaagctc ttgtaaaatt gcctccagtt taccgcaaat cactgggact
     4741 tcttctggca acagttcttt aggtacgtgg aacggttctc caaatgattc aacgattgta
     4801 gtaaagggtt tgggaatttt gtgtctgtcc cagcttttaa gcttccaaag atgttttgct
     4861 tcatagtgcc agggaatgat aggaactcct gagtattttg caagcagtac gactccagat
     4921 tgaatttttt ccggaggacc cagcggacca tccggagtga tagcaactga tgttttgtcc
     4981 ttaagcagtt ttatcagttg tttcagagca ggaattccgc ctctagtggt tgagccgcgt
     5041 actgcctggt agccaaaatg agcaaataca tcgtaaataa cttcaccatc gtcgctgctg
     5101 ctgatcaaag cagttagctc ttgatttctc agaagccaga cactgaaata tatgttttgg
     5161 tgccatgtgg caacaattac gctgccgtat tcctgcagcg cagttttcag tatttgctga
     5221 ttattgcttt taatacgtgt agtatttcca gtaattcgaa tcagcagcat gagcgttttg
     5281 gtgatcagcc agcgctgaaa acgtcttttc aaagaagatt ttgcttcttt catagagaaa
     5341 aaattatttt gttgaatgaa tatgaaagcc actttacaat attacactat catgaacgtc
     5401 atctgctaaa caattcagca gaacctccaa aataatgcag gtaatttctg atgtattaca
     5461 taatccggaa gtccatgtac catcttctga agatgaatgc aataatgaaa gtctaaccca
     5521 aaacaaagtt cttcctgaat ctaagcacaa tgcctatctg atttgttaac cttgtccgtt
     5581 ttttttcaag caaaagcaga agattcgctg acttgtgttt cacctgcccg gagtactttc
     5641 ttccaaatga actggaaaaa ataaatgcat tttcgttcca tagcatacgt cgtggggaca
     5701 ctgctgctgg ttaccagcat tgccctgctg tttcccatca tcacatcctt gatttatcag
     5761 gagaacgatt ttaacgctct gcttatttct gcagtggcgg cttttgggat gggcctgcct
     5821 ttatggtggt tttcaccgaa agattatgaa ttaagcttcc gagacgcagt cctcatcgca
     5881 gtttttggtt gggtaatcat ttccgttgta tcagcactgc cgtttgtgat tcacggctca
     5941 attccgtcat tcacagacgc atttttcgaa atgatgtcag gttacaccac aacaggagca
     6001 actatcctga cggatattga atccctgcct catggattgt tattctggcg cagcgaaact
     6061 catctgcttg ggggtatggg atttctgact ctgaccttga tctttttacc tcacggcatg
     6121 gctggagtac gaatctttcg tgcagagtcc agccccggac aagttataac caaagaaaaa
     6181 tttcttccac gaaaccgtga cgccatgatt tggctctggt ctatttatct cggactgaac
     6241 atgttgcaaa cactgatgct ttggggcggt ggaatgtcac tgtttgactc actgtgtcat
     6301 gcctttggaa ctgttgccac ggcaggattc tctcccaaaa ataccagtct tggctattac
     6361 ggaaatgctt attttgattg ggttgttacg atttttatgt ttcttggcgg aatgactttt
     6421 atgctttttt accacatgct gaaaggagaa tggaatttag ttcgaatcaa cactgaatta
     6481 cgctggtatg ccattattgt tggttttttc tgtggaatat caacactaat tttgtggatg
     6541 aatgggacct atgatcgatt ttttgatgca cttcgccatg cttcttttca aataatttct
     6601 ttgctgacca ctaccggttt cacaactgca gactatgaac tttggcctca gtcagcacag
     6661 atgctgcttt atgttgtctg ctttattggt gcatgtgcag gttctaccac cagcggtatt
     6721 aaaatcgtac attttgttat catctggaaa tacatggtaa caacgatcaa gaaaatgttt
     6781 tttcagcccc ggacaatcct gccgattcta cttaatcagc gtgccataga tccgtttgca
     6841 gtcaatctct ccatctgtta tttcattgcc aacatatttc tcgtcttggg tggaagttgt
     6901 gttatggtgc ttttagattc tatggatttt cagacaggaa tgagttcagt cattgcagca
     6961 gtgatgaacg ttggtccggg atttggtgac gttgggcctt cagaaaacta tgctcatatt
     7021 tcagcagcag gaaaatggtt tcttgcctgg ctgatgcttg ttggcagatt ggaaatgttc
     7081 tctgtccttg tactgctttt tccatctttc tggaaaaaat aatttactgc cgctgtagtc
     7141 cgcaattatt tttccacaca gcttgctgaa caggatttaa actgaatggt tgcaatttca
     7201 gcaagttcag catttaattt tctcaggtat ctcaaagtat tgaaaacagc cctactttct
     7261 gttcccccag cgtaccttga ccatcctttc cggagttaat ttctgtaaat aggcacaacc
     7321 atacttatga attgtgatca ctctctccag agtgagatag ccctgaatgg agtggctttt
     7381 atctggagaa cagcacttcg ctatacgtgt catgacattg tccattccat ccacaagaat
     7441 cttgtttgtt gatggagatt ccgcaattct cttttttctg gtcttggctt tagtttcagg
     7501 ttgtgccggt gattccggag ttgaggcagt aacagaactg gggtcatcgg caaaccatct
     7561 acggacttct tcactgcgaa tagaaccgtc gccaatggaa aaaagtatgt gctcaaattc
     7621 ttgattttta tgtaaacgtg attccttttc catccgccct tcccgaatca gtcgattcag
     7681 attaagactg tgattcttga attcccgttc caacatttcc aaaccttctt ttcgtcttcc
     7741 ttctctgctc cgttcgtgta aagcatgttt aattttattg cgggcgtgac gtgttttcac
     7801 aaaatccagc cactctttcc ggggttcctg tcgcggggat gtaaggattt ccactgtatc
     7861 accgctttgc agggaactgt cgaggcgcgt gatgttacca ttcgtctttg cgccggtcat
     7921 gcggttaccg acttccgtat gaattgcata ggcaaaatca acgggtgtgg cagtttgtgg
     7981 cagttcccgg atatctcttg aaggagtcat tacatagata ttgtcactaa acagatcaat
     8041 aaggggtatt gtttcgttat gcctccctgc tttactttgt tctgccagtg acacttcaat
     8101 ttgttcagct tttttccctt ttgacggttg tcgtttttcc ttatacagcc agtgtgccgc
     8161 aaccccatat tctgccatcc ggtgcatctt atgtgtcctg atttgaattt caattggcct
     8221 taaatatctc aagggaccat tttgggaatc tgtgactgtc gtgtggagag attggtatcc
     8281 gttggactta gggtttgcga tataatcttt gaaacgattt tttatcggcg tccagttttc
     8341 gtgaataaaa ctcagtgccg catagcattc atcgatacca tttaccaaga ctcgaaatcc
     8401 aatcaaatct tgaattcggt caaaattgtt ttcgactttc tcaagttttt gaaaaatcga
     8461 gtaaaaacgt ttgtagcggc cctggacttc atgagtgatt cctgcctctg tcaaaatatg
     8521 acgaattttt tctgaaatta tttcaacact ttctgaacga tcactgcgtt ttcgagcaat
     8581 ttttttcttc aggttttggt aattttcata ttccagttgc ctaaatgcac ggtcctccat
     8641 ctctgccttg atccagaaaa ttcctaagcg tcctgctaag ggagcatgga catgcagagc
     8701 agtccaggct gtttcatctc tctctttcgg agacagggaa tccagctgtt ccaattgctg
     8761 aagctgcagc accaggaaca cagtcaacaa ctggacgtca cccagaactg tcagcagcat
     8821 acgcagggta agttcagcct gctcagaatt tttcggacgt acaggaaact gctgcacctt
     8881 gagcatcctg tgataaagcg gcaatgcatc tgaaaaagag ccctttgaat caattgatgt
     8941 tttgtattca aactctttcg gcaaatcatc ggctgcctga tattgcaaaa acaacaacgc
     9001 agcctggaga gtggcggggc tcaaagaaag ttgttttaga ttctttaaga gtgctgccgc
     9061 agcatcgggg ttgctctgct ttgagtacca gttttcaaaa agactggttg cttccgggct
     9121 tgtcaaaaat gcttccggta caacggaact gtttatatcg gcagaatggc caatagtgat
     9181 tgttggcgac aaaatatctt ctgaaatttc agttccggaa agattaccca tttgaaaggt
     9241 tgctgaatta aatattacaa aagtccctgc accatgaaat aggtgtaaag tttaacatca
     9301 aattggacac cctgcttctt ttcctgcttg aaaaattctg caatttcatc cggagatttg
     9361 acgaatacag ttatgaattc tccgggctca ttttctggtt ttggctgtag gttctctgcg
     9421 atgttttcat caacctgaac ttgtacaaat gaaacggttt ctgaaagaat tccgggagaa
     9481 gaaaacaatc ttgggctaat attttcgact gtaccaacat aacctgtttc ttcaaaaagt
     9541 tcacggattg cagttgtttc ctgagattca ccaggatcaa ctaaacctgc aggaaattcc
     9601 aggatataag aatttgttgg tggacgaaat tggcgaataa taataaatcg tccagaaggt
     9661 tgcattcggg caactataat tactgcctct gcacggtgtt tacgatgcaa tccttcccat
     9721 ttacgtactt gttttttgtc atcttcatag ctgaactcca caatctcggc ccaatttccg
     9781 cgaaagagga gttgttcatc aagaacacgt gctgtgcctg catttggagg attattagtt
     9841 tggatttcag acattcagat tcaacagaaa aattaataaa tgtgtttacg caattggttt
     9901 tgcagtacaa gtggccttat aaaaagatag aggattttgt gacaatcaca atccaatttt
     9961 gaaattgttt ttgcgttgtc ttttgttgat gtctgcaata ccattcgact gatgaaaatt
    10021 aattcctgaa aaccagattc ttgaaacagc cgcccctatg agaagcatcc gcttaatttg
    10081 gattgggaaa tcgcaggagc cttttgttca ggatggaatt aaagtttatt tgaagaaact
    10141 ctcgcattac atcaaggttg attttgaaga aattaagccg gtcaagtata attcaggtac
    10201 gacagatcag tggcgcaggc aggaaacgga aaaattactg aaacgcctgg actcttctga
    10261 attgaacata tttttggatg aaagcgggaa gcgtgaaacc tcaaatagtt tggctcgatg
    10321 gtttgaaaaa caaataccac tcggatggcg gagggtcaac tttttcattg gcggtgcata
    10381 tggttttgac aaaacaatgc tgccttcggg agtaaaattg ttgcgcctct ccgatatgac
    10441 ttttacacat caaatggtga gattaatttt actggagcaa ctttatcg
//
LOCUS       DTVD01000068            2643 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16072_length_2643_cov_224.339645, whole genome
            shotgun sequence.
ACCESSION   DTVD01000068 DTVD01000000
VERSION     DTVD01000068.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16072_length_2643_cov_224.339645"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="EYO68_02490"
     CDS             <1..1032
                     /locus_tag="EYO68_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010443826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC06703.1"
                     /translation="QIEKRAIDIGLFAANLPESVGGGGLDYQSLVLIEREFGKTSHAL
                     HSWIARPAEILMACEGDQVEKYLIPCARAEKRELFALTEPEAGSDIMSMKTNARRDGE
                     DWILNGSKHFISGPVMPDFAIVFAVTGMDETERGSRKRITAFLVDVGTEGFDISEGTR
                     SVSYRGYLTFQLGFSDARLGPGQILGEEGRGLELSGKWLSMNRVWVGASCCGKAERLF
                     NLAAEWAATRKQFGQPIGKFQATGFKLADMAVELKAADLLVMDAVAKGDKGKMSDSDA
                     AMVKLFCSEMLNRTADHTVQIYGGMGLMEEMPIQRLWRDSRLERIWDGTSEIQRHIIS
                     RAILRPLGA"
     gene            1029..>2643
                     /locus_tag="EYO68_02495"
     CDS             1029..>2643
                     /locus_tag="EYO68_02495"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13380.4,HMM:PF13607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIC06704.1"
                     /translation="MNLTQHQNLEKLLKPRHLAIIGGRDAEAVAKECERIGFTGQIWP
                     VNPKREKIGGFKCFASVEDLPEAPDAVYLAIPREAAISTVKRLAEMGASGVVCYTAGF
                     SEIGAEGTELEKALVSASGDLALVGPNCYGVINYIDKVALWPFPHGGASPGYGAAIIT
                     QSGMLSSDLTMSQRSLPFAYMISVGNQSVLRLEDFIDVLCTKPEVKAIGLHIEGVKDI
                     SRFSEVALKALEAGVPIVALKTGSSEIGSELTVSHTASLSGSDDLFQALFDRLGIIRV
                     SHPVQLLETLKYLCVAGIPKGKRLVGLTCSGGGATLLADHAEKIGLDFTRPSKKTAKR
                     LSRLLHYTATVSNPLDYTTPIWGIPERVLPVFETMLTDPYDAAIFVQDYPPPELDESK
                     PFYLSDAKSFIEASSKVRIPSAICCTLPENMDQSTRKMLVENGVAPMQGIHEALEAIS
                     SSVWYFGRREAIRKNGIEKLRIKPLSGISTAGIVSTLIDEWEGKKHLLEIGLSVPEGG
                     IADFAEVPEVAEKIGFPVVVKMVGKNLAHK"
BASE COUNT          673 a          590 c          702 g          678 t
ORIGIN      
        1 cagattgaga aacgcgcgat cgatatcgga ttatttgccg caaatctgcc ggaatctgtt
       61 ggcggaggtg ggctcgacta tcagagtttg gtactgattg aacgggaatt cggaaaaacc
      121 agccatgcgc ttcattcttg gattgcgcgc cctgctgaaa ttctaatggc ttgtgaagga
      181 gatcaggttg agaagtattt gataccttgt gctagggcag aaaaacgaga actctttgct
      241 cttactgaac cggaggcggg ctccgacata atgtccatga agaccaacgc ccgtcgtgac
      301 ggagaggact ggattcttaa cggctcaaaa cacttcattt ctggtcccgt gatgccagac
      361 ttcgccatcg tttttgcagt aactggaatg gacgaaaccg aacgcggtag ccgtaaacgg
      421 attaccgctt tccttgtcga tgtcggaact gagggcttcg atatttccga gggaacccgt
      481 tccgtgagtt atcgtggata tctgactttc caacttggtt tttcggatgc ccgcttaggt
      541 cctggtcaga ttctaggtga agaaggacgt ggtctggaac tatctggaaa atggttgtct
      601 atgaatcgcg tctgggtcgg tgcgagttgt tgcggcaaag ctgaacggct ttttaattta
      661 gccgccgagt gggccgcaac tcgaaaacaa ttcggtcaac ctattggtaa atttcaggca
      721 acaggtttca aacttgctga tatggcggtt gagttaaaag ccgcagatct ccttgtgatg
      781 gatgcagttg caaagggaga taaaggcaag atgtccgatt ctgatgcggc tatggttaaa
      841 ctcttctgtt cggaaatgtt gaaccgcacg gcagaccata cggtgcaaat atatggcggt
      901 atgggtctga tggaggaaat gccgatacag cgtctttggc gagattcccg cctcgaacgc
      961 atctgggacg gaacctcaga aatccagcga cacattattt cgcgtgcgat cctcagaccg
     1021 cttggagcat gaacctgacg caacaccaaa atcttgaaaa actactaaaa ccacgtcatc
     1081 tggcaatcat aggtggccgc gatgctgaag cggttgccaa agaatgtgag agaatcggat
     1141 ttaccggtca gatttggccg gtgaatccga aaagagaaaa gattggtgga ttcaaatgtt
     1201 tcgccagtgt cgaggacttg ccggaagcac cggatgcagt ttatcttgcg attccacgcg
     1261 aggccgcaat ctccacagtg aaacggcttg ccgaaatggg cgcgagcggt gttgtctgct
     1321 atacagcggg gttcagtgaa atcggtgcag aaggcacgga gttggagaaa gcacttgtct
     1381 cagcttccgg agaccttgct ctagttggtc cgaattgcta cggagtcatc aattatattg
     1441 acaaggtcgc cctgtggccg ttcccacacg gtggtgccag tccaggttat ggtgcggcaa
     1501 tcataactca gagtgggatg ctttcttccg acctgacaat gagccagcga tcattgccgt
     1561 ttgcctatat gatttcggta ggaaaccaat cagtgctgag gctggaagac ttcattgatg
     1621 ttctttgtac taagccagaa gtcaaagcca tcggcctgca cattgaaggg gtaaaggata
     1681 tttctcgttt cagtgaagtt gctctcaagg cactggaagc tggagttcca atcgtcgctc
     1741 tgaaaacagg ttcctcggag attggttcgg aattaacggt cagccatacg gcatctttat
     1801 caggtagcga cgaccttttt caggcactct ttgaccgctt gggtatcatc cgtgtttcac
     1861 atccggtaca gctgctcgaa actttaaaat acctttgcgt cgctggtata ccaaagggga
     1921 aacggttggt gggtctgact tgctctggtg gaggtgcaac attgctcgcc gaccatgcgg
     1981 aaaaaatcgg tctggacttc acacgaccct caaaaaaaac cgcgaaacga ctctcccgct
     2041 tgcttcacta cacggctacc gtgtcaaacc cactagatta cacaactcca atttggggca
     2101 ttccggagcg tgtcctacca gtcttcgaga ctatgttaac tgatccctat gatgccgcta
     2161 tttttgtcca ggattacccg cctcctgagc ttgacgaaag taaaccattt tatttgagcg
     2221 atgcgaaatc ttttattgaa gcaagctcga aagtccgtat accttctgca atttgttgca
     2281 cacttccgga aaatatggac caatctactc ggaaaatgtt agtcgaaaac ggggttgctc
     2341 cgatgcaggg tatccacgaa gcacttgaag ccatttcctc ctctgtttgg tatttcggtc
     2401 gccgtgaagc aatccggaag aatggtattg aaaaattgcg aattaaacct ttatccggaa
     2461 tttcaactgc gggaattgtt tcaactttga tagatgaatg ggaggggaaa aaacacttgc
     2521 tggaaatagg attgagcgtg ccggaggggg gtattgccga ctttgcggaa gttccggaag
     2581 tagcagaaaa gataggcttt ccggttgttg tcaaaatggt cggtaaaaat ctggcacata
     2641 aga
//
LOCUS       DTVD01000069            2632 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16165_length_2632_cov_262.416376, whole genome
            shotgun sequence.
ACCESSION   DTVD01000069 DTVD01000000
VERSION     DTVD01000069.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16165_length_2632_cov_262.416376"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            21..2549
                     /locus_tag="EYO68_02500"
     CDS             21..2549
                     /locus_tag="EYO68_02500"
                     /inference="COORDINATES: protein motif:HMM:PF00326.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06705.1"
                     /translation="MNSISCFFHLIKFIPRIMTVMILIQINSFVLASDDLRITKWKIL
                     GPFLGAPRDGRIDHLLSYGGEKKIIPSDNQVFYSALADKGIVTWSELTDQTENIEIKY
                     DNVDWDSVIFRFGSTGLINIGYATTEVTVPEQTRVVVLTRKVPVFFVNSQGYLGEPYS
                     SGFHYTPALLKKGANRILIKFAGKADRHFTFKMKGVHKEAIFIEDITTPDLIQNQVLK
                     PALVGITLANTSLEWMKDLRLKVKGSDDFDESLTSIDPIPPLTIHKFPVRFSQKAVLK
                     SKAPQKVRSLEVELFRGSEQINKHQIKLEIKKIDEPHKKTFFSLIDRSTQYYSILYPQ
                     DFDPDKSYGVIFGLHGASEEASHMAKQYSPKDWAFVVLPTNRRPYGFDWQDWGRLDFL
                     EVLEQVKKHYPVDPERFFLAGSSMGGQGVWHIALHHPSLFAAAAPHAGWANFQTYSPF
                     ILQKSRMFSSPQILMQRNRAMMDSNNLFFLKNAQHLPFIVTQGAKDPVVPPLHARLFQ
                     KTLKELGYQLKYREIADKKHWWNEPLVNGIGADAIDNQEIISFLKGKRLKRYPETLSI
                     HLYDLSINDEFYWIRVLRQHKILDETTLEAKVQENKISLTTKNVAALKMDVDKQLVNS
                     DEVLIEWNGESHRHRLTNGKRAIQLGNDDPERNHTIKKTDYTSLKSVFFKPFVIVYGT
                     RGDESMDQKLLNSARSIASRFWRVANGRTRILRDVDLDNTVIREFNLILLGSQHVNRV
                     TQEIIRDLPVEIGSNRLKMNGRVYPGDVSLAMLYPNPLNPKKMIALFGGTSPDSELLS
                     LYFLPIYSGSGTPDYIIFNHDVLKYAWGGVQAAGFFNHDWDFEN"
BASE COUNT          765 a          590 c          588 g          689 t
ORIGIN      
        1 taaatacaga ttgatccgac atgaattcaa tcagttgttt tttccatttg atcaaattca
       61 ttcctagaat catgacggtc atgattctca ttcaaattaa ttcctttgtc ttggcatctg
      121 atgatctgag aataacgaaa tggaaaatac tggggccatt ccttggggca cctcgagatg
      181 gcaggattga tcatctccta agttatggcg gtgagaaaaa gatcatacca tcagacaacc
      241 aggtgtttta ttctgcgctt gcggataaag ggattgtcac ctggtcggaa ttaacggatc
      301 aaacggaaaa tatagaaatc aagtacgaca atgtcgactg ggattcagtg atctttcgtt
      361 tcgggagtac gggtttgatc aacatcggct atgcaactac tgaagtgacg gtcccggaac
      421 agacaagagt tgtagtgttg acccggaagg tcccggtctt ttttgtcaac agtcaaggct
      481 atctgggaga accttacagt agtggatttc attacacacc tgctctgtta aaaaaaggtg
      541 ccaaccgaat cctgataaaa tttgccggaa aagctgaccg acattttacc tttaaaatga
      601 aaggtgttca taaggaggcg attttcattg aagacataac gaccccagat ttaatacaaa
      661 accaggtatt gaagcctgca ttggtaggta taactctggc caacacaagc ctagaatgga
      721 tgaaagatct cagactcaaa gttaagggtt cagatgattt tgatgaatcc ttaactagca
      781 ttgatcccat tccacctctg accatccaca agttcccggt ccgcttttct cagaaagcag
      841 tcttgaaatc gaaggctcca caaaaagttc gttctctgga agttgaactg ttccgaggca
      901 gcgagcaaat caataaacac caaatcaagc tcgagatcaa aaaaattgat gaacctcaca
      961 agaaaacgtt tttttcactt atcgaccgtt ccactcaata ctactccatc ctttatcctc
     1021 aagatttcga tcctgacaaa agctatggtg tcatattcgg tctgcatgga gcatcagagg
     1081 aagcctccca tatggcaaag caatattcac caaaggattg ggcctttgtc gtcttgccca
     1141 ccaaccgaag accgtatgga tttgactggc aggactgggg ccgcctggat tttcttgaag
     1201 ttttggagca ggtcaaaaag cattatccgg tggacccgga acgcttcttt cttgcaggtt
     1261 catctatggg cggacaaggg gtttggcata tcgcactgca tcacccttcg ttatttgccg
     1321 ctgcggcccc ccatgcagga tgggccaatt ttcagaccta ttctcccttc attctgcaga
     1381 aaagcagaat gttcagcagc ccccaaatcc tgatgcagag aaaccgggcc atgatggaca
     1441 gcaacaattt gttttttctg aaaaacgcgc aacatctacc gtttattgtg acccagggcg
     1501 caaaagatcc tgtcgtccct ccgcttcatg caagactctt ccaaaaaaca ctcaaggaac
     1561 taggctatca actcaagtac agggaaatag cggacaagaa acattggtgg aatgaacccc
     1621 ttgtgaatgg aataggcgcg gatgccatcg acaaccagga aatcatcagt ttcttgaagg
     1681 ggaagcgatt gaagcgttat cccgaaaccc tttcgatcca tttgtatgac ctttccatca
     1741 atgacgaatt ttactggatc agggtgctga gacagcacaa aattctggat gaaaccacgc
     1801 ttgaagccaa ggttcaggag aacaagatta gtctgactac aaaaaatgtt gcggcattga
     1861 aaatggatgt tgacaagcaa ctggtgaact cggatgaagt acttattgag tggaacgggg
     1921 aatctcatcg gcatcgactc acaaacggga aaagagccat tcaacttgga aacgatgatc
     1981 cagagagaaa ccacacgatc aaaaaaaccg attacacttc actcaaatca gtcttcttca
     2041 aaccctttgt gattgtatat ggaacccggg gtgatgagtc gatggatcag aaactgctta
     2101 attcggcaag gtccattgcc tcaagattct ggagggttgc aaacggcaga acccggatcc
     2161 ttcgcgatgt cgatttggat aatactgtca tccgggaatt caacttaatc ctgttgggtt
     2221 ctcagcatgt gaaccgggtg acgcaggaaa ttattcggga tcttccagta gaaatcggct
     2281 cgaacaggtt gaagatgaac ggcagggtct atccaggtga tgtctcgttg gccatgctat
     2341 atccgaatcc gctgaatcca aagaaaatga ttgcactatt cggtgggacg tcacctgatt
     2401 cagaacttct ttccctttat ttccttccca tctactctgg ttctggcact cccgattaca
     2461 ttatctttaa ccacgatgtg ctcaaatatg catggggcgg agtgcaggct gccggttttt
     2521 tcaatcacga ttgggatttc gagaactgaa ccgaggagac tgttacagaa aggctatatt
     2581 tgctgaaaca gtaacttttg ctgtcctgaa ttcaagtatc taccctgctc at
//
LOCUS       DTVD01000070           10406 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1624_length_10406_cov_435.595401, whole genome
            shotgun sequence.
ACCESSION   DTVD01000070 DTVD01000000
VERSION     DTVD01000070.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_10406_cov_435.595401"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            93..1385
                     /locus_tag="EYO68_02505"
     CDS             93..1385
                     /locus_tag="EYO68_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIC06706.1"
                     /translation="MTSRFWFLTYNIILIPLLSVVVKILASYHSKINESIEKRKGLWE
                     RLEEAVSKRDWQKPLIWFHVASAGEFLQAQPLIVRCIAEGAECAVTYSSVNAYHWLQR
                     PDQTETQNLLAAEFLPVDKIYNVRRLLGLLQPSRLVWVSYDLWPNLVWETYRQGIPQS
                     LVSGIIHGGSLRTSFFAGRSFYHSLYECLEHILTVSEADSQRVLSAIPNHPLVKVMGD
                     TRCDSVLERRDNIEIPQLPPAAKDSFVFVAGSTWPQDESCIFSGLQEALNKFPELFLV
                     LAPHEPTEEHLKNAENFFSGTPMVRWSHVTAAALDVRILLIDSVGILAGLYHSAKMAY
                     VGGAFTTGVHNILEPVAMGAAVAFGPKHSNSMEALTMLEQKLATTVNNSTEFRNWIFD
                     SLGNQEHCILLGRQSKKFVETQAGAADLCTPFLMNGLS"
     gene            complement(1397..1849)
                     /locus_tag="EYO68_02510"
     CDS             complement(1397..1849)
                     /locus_tag="EYO68_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIC06707.1"
                     /translation="MSPLLGRLLALSFQNSNWLEKYDILIPIPLHSSRLRKRGFNQSL
                     LLAYYFKKNLGKSAPELQTHWLRRIRATRPQTELPLAERLVNIDDAFGTSLEVQNHHI
                     LLLDDVMTTGSTLNAAARCLKEAGASSVGALVLGRKMWDSFEDGFTDA"
     gene            complement(2319..2891)
                     /locus_tag="EYO68_02515"
     CDS             complement(2319..2891)
                     /locus_tag="EYO68_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIC06708.1"
                     /translation="MQANNTILEAPPKVGSEIQRLRLKHNLTLEQLAVKSGVSKSILS
                     QIERDLSNPTLATIWRITKALESPLEDILSVKEGTTCFEKLSQNATPEVMSEDSGFRL
                     KILGTLNTVSTVQWYEFTAEPKAELVSEAHGTGSLESLTLCTGELTVIVGDETQYICK
                     GDTLRFRTDVAHRLKNEGKEKAHGFMVNLL"
     gene            3118..3396
                     /locus_tag="EYO68_02520"
     CDS             3118..3396
                     /locus_tag="EYO68_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HIC06709.1"
                     /translation="MGVVIEIPSALKQYVNNQDEVEVQGNSVEEAFDSLCKDFAELKP
                     NLFDENGKVRNFINVYLNDDDIRYADGMNSSVQDGDCIQIVPSVAGGR"
     gene            3427..3990
                     /locus_tag="EYO68_02525"
     CDS             3427..3990
                     /locus_tag="EYO68_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinucleotide-utilizing protein"
                     /protein_id="HIC06710.1"
                     /translation="MKLNNEEINRYSRHLTLPEVGMAGQLEIKESRVLMIGAGGLGSP
                     LGMYLGAVGVGKIGLVDFDVVDHTNLHRQIAHTTADEGRPKVESLRDTILAGNPNIEV
                     EIHNTRLERDNVLDLFSQYDIIADGSDNFETRYLINDAAFFSKKPLVSASIFRFQGQI
                     TIFSPETGGPCYRCLYSEPPPAALVPS"
     gene            4012..4584
                     /locus_tag="EYO68_02530"
     CDS             4012..4584
                     /locus_tag="EYO68_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06711.1"
                     /translation="MLPGVVGLIQATEIIKLILEGGAPLIGRLLLYDAMKMNFKEVRV
                     RKDPECALCGEHPSVTELIDYKAFCDVPLPGAELPEEIDEAEFELSPREFKDAMDQDP
                     SAVLVDVRETFEWDICRIDGAQLMPLSSFDPTTTGLDPEARIYLYCYKGKRSMLALKE
                     LKRAGFNKLKNLSGGIYLWAEEVDSDMPQY"
     gene            4646..6901
                     /locus_tag="EYO68_02535"
     CDS             4646..6901
                     /locus_tag="EYO68_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIC06712.1"
                     /translation="MVIPENILAQCHMHGVEAYPEETCGFIVGNREDQDSLETVLPMR
                     NIMNELHEEDPEQYPRTARDGYMIDPREQMILERSLKKEGKEIKVIYHSHPDVGAYFS
                     EKDKEDAMWNGKARYPGVTFLVCATTKGKPDGAILADFNENSGDFDITPVLPDSSSTS
                     AGLVGGTYGVTGILPTIKFIHEWKNGNRQLEHGYFRFVPPRQVRDLQQELSARSNGRH
                     ALVYCSGRTALFELLVYLRESRPQTNLHFSINSTFLSESLHNLEIPCHLLDIENLIEP
                     ENLSAKQGDVLLLEMKDPETFLRKESEWFGNLKRLRVPVIIFAACPPDFEVWPGGLTY
                     WVSNIKTPDSSSDISGIEGGVILSSADRQIAELTEIRKRNGPILSARNAAVFLEFFLE
                     NKIDLKHISVLSGIENRQKVSSTESLISQRLCEWEHASSGFLFPSGMSAIFAVLNLLR
                     KKEKPQVIVIGLLYSESYTLLMDSGLSSNWEAIFLGLAELERLPEILSDKTAMIITET
                     ITNPLGEVPDLEKIGNIANSFGVPFVVDSTLASPANCQPMDYGVDYVIHSTTKYLSGS
                     NDHGGGVVLVKSNEDALALENFQKYWGLTISPLESVVLLECMQDFEERMERFNTNGTE
                     IAKFLSEHSAVAHVYHASLPSHSSFSTAKKLLSGIGGVVSFTLKDESEEGLQNFHDKD
                     FSAILKAPTLGSNQTLICPYPMLTHYFDTDQELEEIKLPRHLIRIAAGCEKDLAPILG
                     DLNGALLRTIR"
     gene            complement(6891..7181)
                     /locus_tag="EYO68_02540"
     CDS             complement(6891..7181)
                     /locus_tag="EYO68_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIC06713.1"
                     /translation="MVKHIVMFKLKEKNAENMEAAVSTLRGLDGKIESLRFIEVGVDF
                     KETERSYDLVLTTHFDDQEGLSAYASHPTHLPVIETMRNLCSNSIVVDYVTE"
     gene            complement(7190..8422)
                     /locus_tag="EYO68_02545"
     CDS             complement(7190..8422)
                     /locus_tag="EYO68_02545"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIC06714.1"
                     /translation="MFEEFKKRGFFQQCTHSEELAKKLKEDRICCYVGFDPTADSLHV
                     GHLIPLMGLAHMQRAGHRIIALMGGGTAMIGDPSGKTEMRKMLTEEQLQSNIEGMLPQ
                     FERFLDFSGDAILINNVEWLRNLNYIDFLRDIGRHFSVNRMLSFETYKIRLESGLSFL
                     EFNYQLLQAYDFLELNRRYGCSLQMGGDDQWANIISGTDLIRRVERKEAYGWTYPLLT
                     TSSGKKMGKTEKGALWLDPKRTSAYEYYQYWVNTEDDDVGKFLALFTFLPMKEIRELG
                     KLQGADIREAKKRLAFETTALIHGEENSLQAQQSAQALFSGNSGEGSGDKSNVPSTTI
                     AAASLSAGLGIFEIFQETGLCKSKGEARRLQSQGGVYVNDERIDDPDFCLGEADLRDG
                     EILLRAGKKRYHRLIKES"
     gene            complement(8425..9030)
                     /gene="recR"
                     /locus_tag="EYO68_02550"
     CDS             complement(8425..9030)
                     /gene="recR"
                     /locus_tag="EYO68_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HIC06715.1"
                     /translation="MQIPVALERLVFEFSRFPGVGRKTAQRLAFNILRYTTEETQNLT
                     DALTQVKEQIRYCSVCSGFTDIDPCAICSHSSRDQQQVCVVEQPNNIFPIEKSGVFKG
                     VYHVLMGAISPLDGIGPEQLNVKKLRNRIENNKISELILATNPTVKGEATALYLQQEF
                     AGKISTITRLACGIPAGGDLEYVDDVTLMEAFYGRHIVNNE"
     gene            complement(9037..9552)
                     /locus_tag="EYO68_02555"
     CDS             complement(9037..9552)
                     /locus_tag="EYO68_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06716.1"
                     /translation="MSNTPAKIINLADRRARKEDESRNAPIPGWIIWLHCPKCKSLEY
                     SEIEMPDGRVHKCGTLVEEEEVQIDVRAEYTISLRNSLRLDELFKQTKIPGFLKPLAK
                     KGIGMLENLQAAEEEYRKRLKNITGGSVDAYSNDWDEKSLGMELKTLEPLGIILTEAR
                     QPNLHFPEVGS"
     gene            9967..10338
                     /locus_tag="EYO68_02560"
     CDS             9967..10338
                     /locus_tag="EYO68_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06717.1"
                     /translation="MKKLFYVLLISIFCMGIVSCANTYTKIIKSKAINTVFDEISEAS
                     GSTLVDSTVEESSIKDSTITKSKILANSKIMNKSIIINSTIENSTISNSEIINQTITN
                     QIITNSKIEGPAKEEEAAKEE"
BASE COUNT         2958 a         2156 c         2187 g         3105 t
ORIGIN      
        1 attcttttgc ctgttctaat caaagttttg ccttttaaca ttcttcccag ttcatttcag
       61 gatgaacctg tttcccagca agaactatct caatgacttc ccgtttttgg tttctgactt
      121 acaatattat tttaattccc ctgctgtcag tagtagttaa aatattggca tcctatcatt
      181 caaaaatcaa cgagagcatt gaaaaaagga aaggtttgtg ggaacgtttg gaggaggcag
      241 tttcgaaacg agactggcaa aagcctttaa tctggtttca tgtagcaagt gctggagagt
      301 ttttgcaggc acagccgctg attgtccgct gcattgcgga aggtgcagag tgtgctgtaa
      361 cctacagttc agtaaatgct taccactggc ttcaacgacc cgaccaaacc gaaacgcaaa
      421 atttgctggc agcagagttt ttgccagttg ataaaatcta caacgttcgc cgtttactgg
      481 gtttgctgca gccctcgcgt ttagtctggg ttagttatga tttgtggccg aatttagttt
      541 gggaaacata tcgacaaggt atccctcaat cactggtttc cggcattatc cacggtggtt
      601 ccttacgtac ttcctttttt gctggccgct ctttttatca ttcactttac gaatgcctgg
      661 agcatatttt aactgtttct gaagctgaca gtcaaagagt actgtctgct atcccgaatc
      721 atcccctagt caaagtaatg ggtgatacac gttgtgacag tgtgctggag cgcagagata
      781 atattgaaat tccacaattg cctcctgctg caaaagacag ttttgtattt gttgccggaa
      841 gtacctggcc tcaggatgaa tcatgtattt tttctggact gcaggaagct ctgaacaaat
      901 ttcctgaatt gtttctggtt cttgcaccac atgaacctac cgaagaacat ttgaaaaatg
      961 cagaaaactt tttttctgga actcctatgg tacgctggtc acatgtcaca gcggctgctt
     1021 tggatgttcg gattctgctg attgattcag ttggaatcct ggcaggactt tatcattctg
     1081 caaaaatggc ctacgtggga ggagcattta caaccggtgt tcataatatt cttgaaccgg
     1141 ttgcaatggg agccgcggtt gcctttggtc caaaacacag caattcgatg gaggccctga
     1201 caatgcttga gcagaagctg gcaactaccg ttaataattc aacagaattc cgaaactgga
     1261 tttttgattc tttagggaat caggaacatt gtattctatt gggaaggcaa tccaaaaaat
     1321 ttgttgaaac acaagccgga gctgcagatc tctgtacacc atttttgatg aacggtctgt
     1381 cctaattttt tgtgtttcag gcatcagtaa atccatcttc aaatgagtcc cacattttcc
     1441 tgcccagcac aagtgcaccg acactgcttg cacccgcttc tttaaggcac cttgctgctg
     1501 cgtttagagt cgaaccggtt gtcatcacat catcaaggag cagtatatga tgattctgaa
     1561 cttccaaaga ggtcccgaaa gcatcatcaa tatttaccag cctttccgcc aaaggcaatt
     1621 ctgtttgggg acgggtagct cgaatacgtc tcagccaatg agtctgtaat tccggtgcag
     1681 acttacccag atttttctta aagtaatacg ccaaaagaag agactgattg aagccccttt
     1741 tacgcagacg agaggagtgg agaggaattg gaatgagaat atcatatttt tccagccagt
     1801 tgctgttttg aaaactcaac gctaaaagac gtcccagcag tggagacaaa tgttctttac
     1861 catagaattt cagctgcaaa atccaatgcc ggacaatatc atcgtaggca aaaacacttc
     1921 gggtttcatc taattcccaa atatcctcaa tacattcctg acagcggaga cgatttggtt
     1981 cagcagtgtg atttccgcat tgtcggcaat gataagcctg atctgtccat ggaagttcaa
     2041 gcagacagct ggagcagaga aacggagagg ctggatcaag gaaagttctt ccgcccagaa
     2101 cctttttaca ttgccagcag agtggaggaa gcaattgcct taaccaccgt aaccatcgcg
     2161 gcaatggaga aggatgacgg agagatttga caaattcttc cattcaagcc ttgaagagga
     2221 gggtcggtaa gtctaatttt cttgattccc tctttcgggg atatgacaac ttttattact
     2281 tttgcagcaa caagaatttc ctgaggacag gattattttt agagtaaatt aaccataaat
     2341 ccgtgagcct tttccttccc ttcatttttc agtcgatgcg caacatcagt acggaaacga
     2401 agtgtgtctc ctttacaaat atattgtgtc tcatcaccaa ctattacggt caattcacct
     2461 gtacagaggg tcaaactttc aagacttccg gtcccatgtg cttcagaaac taattctgct
     2521 tttggttctg cggtaaattc ataccactgt actgtagaaa ccgtatttag agttccaagg
     2581 attttcaagc gaaatccgct gtcttcgctc atcacttccg gtgttgcatt ctggctcaat
     2641 ttttcaaaac aagttgttcc ttcttttacg gagagaatat cttcaagagg actttccaga
     2701 gctttggtaa tgcgccaaat tgtcgctaaa gtcggattac tcaaatcacg ttcaatttgt
     2761 gaaagaatag attttgaaac tccggacttg acagctaact gctccaaagt aaggttgtgc
     2821 ttaagtctca gtcgttgaat ttcggagccg acctttggag gagcttccaa tattgtattg
     2881 tttgcttgca tatataatct tatctgatta catttgacaa acaggtattg tattttgact
     2941 ttttacctgt tcatcaaata aagaaaaaat tcattttcaa gtgatttttt acttgtatga
     3001 tcgttattta atgttatatt ctttatattg gattggtgtt taatataaca tacttatcca
     3061 ttatactaag agtataaacg atcaattaat ccattatcca gacaaaaaag gagaactatg
     3121 ggagtagtta tcgaaatacc atcggcacta aagcagtatg tgaataatca ggacgaagtt
     3181 gaggtccagg gtaattcagt tgaggaggca tttgattctt tatgtaaaga ctttgccgag
     3241 ctgaaaccaa atttatttga cgaaaacgga aaagtccgta attttatcaa tgtctatctc
     3301 aacgatgatg acatccgtta tgcagacggc atgaacagtt cagtgcaaga cggagattgc
     3361 atccagatcg taccgtctgt cgcaggaggc agataaactg taaaatttgt ttaatggagt
     3421 attaaaatga aattaaataa tgaggaaatc aaccgctaca gcaggcacct gactttgccg
     3481 gaagttggta tggcaggtca gttggaaata aaggaatccc gagtgctgat gattggtgca
     3541 ggaggacttg gctctccgct gggcatgtat ctgggggcgg ttggtgtcgg caaaattgga
     3601 ctggttgatt tcgacgtagt ggatcacacc aatttgcatc gccagatcgc acatacgact
     3661 gcagacgaag gacgtccaaa agtcgaatca ctgcgagata ctattttggc aggaaacccc
     3721 aatattgaag ttgaaattca caacactcga ttggagcgtg acaatgtgct cgatttgttc
     3781 agtcagtatg acatcatcgc agatggttcg gataattttg agacacgtta cctgatcaat
     3841 gatgctgcat ttttttccaa aaaaccgctg gtttctgctt ccatttttcg ctttcagggt
     3901 caaatcacaa ttttttctcc tgagaccgga ggcccgtgtt accgttgtct ttattctgaa
     3961 cctccgccgg cagcgctcgt tccgagttga gctgttgcag gtgtcctggg cgtgctcccg
     4021 ggcgtagtcg gtctgattca ggcaacagaa ataattaagc tgattttgga aggaggggcg
     4081 cctcttattg ggcgattgtt gctctatgac gcaatgaaaa tgaacttcaa agaggtccgt
     4141 gtccgcaaag acccggaatg cgctctttgt ggagaacatc ccagtgtaac agagttgatt
     4201 gattacaaag ccttttgtga tgttcctctt cccggagctg aacttccgga agagattgat
     4261 gaagcagaat ttgagctttc tccccgggaa tttaaagacg cgatggatca agacccatct
     4321 gctgttttgg tagatgtgcg tgaaacattt gagtgggata tttgccggat agacggcgct
     4381 caacttatgc cgctgagtag ttttgatccg acgaccaccg gattggatcc ggaggcgagg
     4441 atttatctct attgttacaa aggtaaacgc tccatgctgg ctcttaaaga actcaaaagg
     4501 gccggcttca ataagctgaa aaatttgagc ggcggcattt atctctgggc ggaagaggtc
     4561 gattctgata tgcctcagta ctgaaactag ggggcattcg tgtgcgccaa tttcccctaa
     4621 acaactcaag aattttttga accagatggt tattcctgaa aatattttgg cacaatgtca
     4681 catgcatggt gtggaggcat atccggaaga gacttgcgga tttatcgtgg gaaatcgtga
     4741 agatcaagat tcccttgaaa ctgtgttgcc gatgcggaat atcatgaatg aattgcatga
     4801 ggaagatccg gaacagtatc ccagaacagc tcgagacggt tatatgattg acccgcggga
     4861 gcaaatgatt ttggaacgtt ccctgaaaaa agaagggaaa gagatcaaag ttatttacca
     4921 cagtcatccg gatgtgggtg catatttttc tgaaaaggac aaagaagacg caatgtggaa
     4981 cgggaaagca cgttatccgg gagtcacatt tttagtttgt gcaactacca aagggaaacc
     5041 ggacggcgcg attttagcgg attttaatga aaacagcgga gattttgata ttacaccagt
     5101 tcttccggat tcatcttcaa cgtctgccgg attggtggga ggtacttatg gagttactgg
     5161 aattttgccc acaatcaaat ttattcacga atggaaaaac ggaaaccgtc agctggaaca
     5221 cggatatttt cgttttgttc cacctcgcca agtcagggat ctacagcaag aattgtctgc
     5281 ccgctccaat ggccggcacg cactggtata ctgttccgga cgcacggctt tattcgaatt
     5341 gctggtatac ttgcgggaat cacgtcctca aacaaatcta catttttcta taaattcaac
     5401 cttcctctca gaatctctgc acaatttaga aataccctgc catttacttg atatagaaaa
     5461 tctgattgaa ccggaaaatt tgtctgcaaa acaaggtgat gttttgctgc ttgaaatgaa
     5521 agatccggaa acatttctca gaaaagaatc cgagtggttt ggaaatttaa aaaggcttag
     5581 ggtacccgta attatttttg cagcatgtcc gcctgatttt gaggtgtggc ccggaggtct
     5641 gacttactgg gtctcaaaca tcaaaactcc ggactcaagt tctgatattt ccggaattga
     5701 aggtggtgtt attctgagta gtgcagacag gcaaattgct gaattgactg aaatacgcaa
     5761 gcgtaacggc cccatactgt ctgctcgaaa tgctgccgtt tttctcgaat ttttcctgga
     5821 aaataaaatt gacttaaaac atatttctgt actctccgga atcgaaaaca gacaaaaagt
     5881 ttcaagtacg gaatcactca tttctcaaag gttgtgcgaa tgggaacacg cttcttccgg
     5941 attcttattt ccctcaggaa tgtcagctat ttttgcggta ctgaatttac tgcggaaaaa
     6001 agaaaaacct caggtaattg tgattggatt actctacagc gagagctata ctctcctgat
     6061 ggattctgga ttgtcatcaa attgggaggc catcttcctg gggttggctg aattagaaag
     6121 attaccggag attttgagtg ataagacggc aatgattatc actgaaacga tcacaaatcc
     6181 actgggggaa gttccggatt tagagaaaat aggtaatatt gcaaattctt ttggagttcc
     6241 tttcgtggtc gatagtacgc ttgcttctcc ggcaaattgc cagccgatgg actacggtgt
     6301 ggattatgtg attcacagca cgaccaaata tctgagtggc agcaatgatc acggaggagg
     6361 agttgtactg gtgaaaagta atgaagacgc acttgccctg gaaaactttc aaaagtattg
     6421 gggactgaca atatcccctt tagaatcagt agtattattg gagtgcatgc aggattttga
     6481 agagcggatg gaacgcttta acacaaatgg gacagagatc gctaaatttc tgtcagagca
     6541 ttccgcggtg gctcatgttt atcacgcatc tctgccgtca cattcttctt tttctactgc
     6601 aaaaaaacta ctttcgggta ttggaggagt tgtcagtttt accctgaaag atgaaagtga
     6661 agaaggtttg cagaatttcc atgacaagga cttttctgcc attctcaaag ctccaaccct
     6721 tggttccaat caaaccctca tttgtccata tccaatgctg actcactatt ttgatacaga
     6781 tcaggagctt gaagaaatta aacttccacg acacctgatc cggattgcgg caggttgtga
     6841 aaaggatctt gcaccaattt taggggattt gaatggtgcg ttgctgagga ctattcggta
     6901 acataatcga caactattga gttggaacag aggttccgca tagtttcgat tacgggcaaa
     6961 tgggtgggat gtgaagcata tgcagacagt ccctcttgat catcaaaatg tgtggtgaga
     7021 accagatcat aggaacgctc tgtttccttg aaatcaactc caacctcgat aaatctcaga
     7081 gattcaattt ttccatccaa acctctcaat gttgaaactg ctgcttccat attttcagca
     7141 tttttttctt tgagtttgaa cataacaata tgcttgacca tattttctcc taagattctt
     7201 taattaagcg gtgataacgt tttttacctg cccgaagaag aatttctccg tcccgtaaat
     7261 cagcctcacc aagacaaaaa tccggatcat caatccgttc atcatttacg taaaccccac
     7321 cttgtgactg cagacgtcgt gcttctcctt tggatttgca aagacctgtt tcctgaaata
     7381 tttcgaaaat cccaagtcct gcagataaac tggctgcagc aattgtggtg ctgggtacat
     7441 ttgatttgtc tccacttcct tcccctgagt tgccggaaaa aagagcttgc gctgactgtt
     7501 gggcctgaag tgagttttct tcgccgtgaa tgagcgcagt tgtctcaaaa gccagtctct
     7561 ttttagcttc acgaatgtct gcaccctgaa gtttacctag ttccctgatt tctttcattg
     7621 gcagaaaagt aaataaggcc aggaattttc ctacatcgtc atcttccgta ttgacccaat
     7681 actggtagta ttcataagct gatgtacgtt taggatcaag ccagagagca cctttttcag
     7741 ttttccccat cttttttccg gaggaggtag tcaagagcgg atatgtccag ccgtaggcct
     7801 cttttcgttc cactcggcga atcaaatcag ttccggaaat gatgttggcc cactgatcgt
     7861 ctccacccat ttgcaggctg caaccataac gtcgattcaa ctccagaaaa tcgtaggcct
     7921 gtaaaagctg ataattgaat tccagaaagc ttaatcctga ttccagtcta attttgtatg
     7981 tttcaaaaga cagcatccgg ttaactgaaa aatgccgacc aatgtctcgc agaaaatcta
     8041 tgtaattcag attacgcagc cattccacat tattaattag aattgcgtct ccactaaaat
     8101 ctaaaaaccg ttcaaattga ggaagcatcc cctcaatatt tgattgcagc tgctcttcag
     8161 tcagcatttt tcgcatttcg gttttaccgc tgggatcgcc aatcatggcc gttccgccgc
     8221 ccattaaggc aataattcta tgacctgctc tttgcatatg agcaagtccc ataagtggaa
     8281 tcaggtgccc gacatgtaat gagtctgcgg ttggatcaaa tccaacatag cagcagatac
     8341 ggtcttcttt taatttttta gccagttcct cggaatgagt acactgttga aaaaaaccgc
     8401 gttttttaaa ctcttcaaac atacttattc attattaact atgtgcctgc cgtaaaaagc
     8461 ttccatcaga gttacatcat ccacgtattc cagatcacca cctgcaggaa tgccacaggc
     8521 aagccttgta attgttgaaa tttttccggc aaattcctgc tgtaaataca aagcagttgc
     8581 ttcacctttt actgtaggat ttgtggctaa aatcaattcg ctgattttat tgttttctat
     8641 cctgtttctt aattttttca cattcaactg ttccggtccg atgccgtcaa gtggagaaat
     8701 tgcacccatc agaacatggt aaacaccttt gaatactccg cttttttcaa ttgggaaaat
     8761 attattcggc tgttctacca cacacacttg ctgctggtca cgtgaggagt gactgcatat
     8821 tgcgcatgga tcaatatctg tgaaacctga acagacagag cagtaacgaa tttgctcttt
     8881 gacctgtgtc agggcatccg tcaggttttg agtttcttca gtagtatagc gaagaatatt
     8941 aaaggcgagc cgttgtgctg tttttcggcc aacacccgga aaacgagaaa actcgaaaac
     9001 cagacgttca agagcaacag ggatttgcat gagaaatcag gatcctactt ccggaaaatg
     9061 cagattgggt tgtcgtgctt ctgttagaat gatcccgagt ggttccagcg ttttcagttc
     9121 cattcccagg gacttctcat cccagtcatt agaataagca tcaactgaac cgccggttat
     9181 attttttagc ctctttcggt attcttcctc agcagcttgc agattttcca gcatgccaat
     9241 accttttttt gccaggggct tgagaaatcc gggaattttt gtctgcttaa aaagttcgtc
     9301 tagtctgagt gaattacgca gtgaaatagt atattctgca cgcacatcta tctggacctc
     9361 ctcctcttcc accaatgtac cgcatttatg aactcttcca tccggcattt ctatttctga
     9421 gtattccaga gacttgcatt taggacaatg cagccaaatt atccaacctg gaataggtgc
     9481 atttcgagat tcatcttctt tcctggcccg acggtccgca agatttatta ttttggcagg
     9541 cgtgttgctc atttttgaca gtgaaaaata ctggacctta gaaattaagg agctaaaata
     9601 ttatattcaa ttattgaata atcagtcttg attctcactt attttacaat ttatgtcaaa
     9661 atattttaaa aatagaacac acatcctaca ctttaaatca gtacataaac actaactgct
     9721 aaagttaaaa aaccttccac taagtacccg actgcaaaac atttaaatca aacaatcaat
     9781 tttttcttga catactctgg atgatattat atcttcaaca actatgaatc attcttccct
     9841 ttggtctgaa tgttcagcta aatggtttgg tagctaattt tattgtgcga aacagtccct
     9901 ttgattgtga agtataaaca tttgatttat caaactttct cttcattata agtaacggag
     9961 gaatagatga aaaaattatt ttatgtttta ttaatttcta ttttctgcat gggaatcgtt
    10021 tcctgtgcaa atacttatac gaaaataatc aaatcgaaag caataaatac agtttttgat
    10081 gaaatttctg aagcatccgg ttcgacactt gtagattcaa cagttgaaga atcttctatt
    10141 aaggattcga ctataactaa gtcaaagatt ttggctaatt ccaaaattat gaataaatct
    10201 attattataa attcaaccat cgaaaattcg acaatatcaa attcagaaat aataaatcaa
    10261 accataacaa atcaaataat taccaattca aaaatagaag gaccagctaa agaagaagag
    10321 gcagcaaaag aagagtgatt tcagtcattc agtagtattt ctactaaatt ctttggtgaa
    10381 aaaactgtaa aatcactcga aaccgt
//
LOCUS       DTVD01000071            2619 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16308_length_2619_cov_210.275351, whole genome
            shotgun sequence.
ACCESSION   DTVD01000071 DTVD01000000
VERSION     DTVD01000071.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16308_length_2619_cov_210.275351"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            97..222
                     /locus_tag="EYO68_02565"
     CDS             97..222
                     /locus_tag="EYO68_02565"
                     /inference="COORDINATES: protein motif:HMM:PF13385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06718.1"
                     /translation="MPKNNGIKEAASEYLKGSIDEVRIYERALSAQEITDLYTQQ"
     gene            complement(562..750)
                     /locus_tag="EYO68_02570"
     CDS             complement(562..750)
                     /locus_tag="EYO68_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIC06719.1"
                     /translation="MEDTKPKIIIGNKGPLVIKGGVTLIHPDGLEEQRDGTVALCRCG
                     LSAKKPFCDGSHKGCDTW"
     gene            1000..1863
                     /locus_tag="EYO68_02575"
     CDS             1000..1863
                     /locus_tag="EYO68_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HIC06720.1"
                     /translation="MPKNNFVFLTFLMILSISLMPFMEFRALAQANNPVSSETVQDTS
                     SETDTDEEEDAFDAEFEDEFGDAEKEVFDPLSGYNSVMTNFNDGFYIYVLDPVARGYR
                     WVLPDTARRGVKSFFHNLLFPLRFVNNALQLKVKNAGDEFLRFSINSTIGILGFWDPA
                     KEWFGLEAHEEDFGQTLGFYGVGGGFHVVLPFLGPSNVRDMFSLYPDMQMDPVNYVET
                     RPYNFPKQEGEYLGVSRQTLQQTELTLLKTINRESLRIGQYENLKKDAIELYPFLRDV
                     YEQNRAKLIKE"
     gene            1866..2483
                     /locus_tag="EYO68_02580"
     CDS             1866..2483
                     /locus_tag="EYO68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC06721.1"
                     /translation="MQIINKKIMYRTLISLFAVFFAAATLSADEVNEIRAMTKEKVDL
                     VIQTLKDTSLSKKEKKEGILKTIDGLFDFNLMARLSLGKENWKPLSKSKRKEFSKLFV
                     ERLKQSYLDKLDLYTDEEVVVGEAKQTKKNRVEVLTFLVSKDDKKEMTYKLYKSKKKG
                     WLVYDVDVLGVSIVATYRSQFSGILKKGSMEQLLERMRSTGELGS"
BASE COUNT          847 a          442 c          539 g          791 t
ORIGIN      
        1 actcaaaacc gatggttgag tccttaaacg tgtcgctaaa ttaaaagtgt ctgcatagct
       61 tagactgacc aaactcttca cccaaaccgt cttccaattc cgaaaaacaa tggaattaag
      121 gaggccgcaa gcgaatatct gaaaggtagc attgacgaag tccgcatcta cgagcgcgcc
      181 ctgagcgccc aggaaattac tgacctttat acacagcaat agtgaagatt ttaaaaatag
      241 aagggacaca tctaagtgtt tcaggggaca aaatttgaaa aaaatgtgat tgataaaaaa
      301 ggggatacaa aagtcctgat tccagctgtg aatcagaaga tgcaactgaa aaggctatac
      361 cattattagt ctgtaacgct gttaccgtta ctatatcctt tggtgaaatt agtaattatt
      421 aaaatgctac ctgtcactta aaattatttg gaagtactga tggtggtaga tataaatcat
      481 tgaaaaataa ataataaaat caaaattata tattaaaata ggtacctatt catttccgcc
      541 aattatagaa tatttgtctg ttcaccaagt gtcacacccc ttgtgagagc cgtcacaaaa
      601 tggttttttg gcagaaagcc cgcagcggca gagggctacc gttccgtccc gttgttcctc
      661 aagaccatcc ggatgtatta aagtaactcc acctttgata actaaaggcc ccttgttgcc
      721 tataatgatt tttggtttgg tatcttccat aaattttttt tatttgtgat gaaataaata
      781 tctatctgta gattggaata attacatttc ttgctaaaga ttttgattgt tgagacaaac
      841 tgattgttga aaatcaatta aatttgaaat taactttaaa ctcataaaat aatccggaat
      901 tgattttatt cgatattcag cccgtaggta ataatcttgc acttggctag aaaaaagtaa
      961 attctattta gtggaaaaga aaattataat cgccatccaa tgccaaaaaa taattttgta
     1021 tttttaacat ttttaatgat tctgtctatt tcgttaatgc cctttatgga attccgggct
     1081 ttagctcagg caaacaatcc ggtttcaagc gaaacagttc aggatacgtc gtctgaaaca
     1141 gacacagatg aagaagagga tgcgtttgat gctgaatttg aggacgagtt tggagatgct
     1201 gaaaaagaag tttttgatcc tctgagtgga tacaacagcg taatgacgaa ttttaacgac
     1261 ggtttttata tttatgtttt agatccggtt gctcgtggat atcgctgggt acttccggat
     1321 acagcacgta gaggtgtgaa aagttttttt cataatttgc tttttccttt gcgctttgtc
     1381 aacaatgctc tccaacttaa agtaaaaaat gccggcgatg aatttcttag attcagcatt
     1441 aacagcacca ttggaatttt aggtttttgg gatccggcca aggagtggtt tggtttagaa
     1501 gcccatgaag aagattttgg acagacactt ggattttacg gtgttggagg cggattccat
     1561 gtggttttac cttttttggg tccctcgaat gtacgggaca tgttcagcct gtatccggat
     1621 atgcaaatgg atcctgtgaa ctatgttgaa actcggcctt ataattttcc caaacaagaa
     1681 ggagaatatc tgggcgtatc cagacagaca cttcagcaaa cggaattaac acttttaaaa
     1741 actattaacc gcgaatctct gcgtattgga caatacgaaa atcttaaaaa agatgctatt
     1801 gaactctatc cttttttgag agatgtctat gagcagaatc gagctaaact aatcaaggag
     1861 taatcatgca gattattaat aaaaaaataa tgtaccgaac tttaatttct ctttttgcag
     1921 tctttttcgc tgcagcaact ctgtctgcag atgaggtcaa cgaaatccgg gcaatgacca
     1981 aggaaaaagt tgaccttgtt attcaaaccc tcaaagacac aagtttgagt aaaaaagaaa
     2041 aaaaggaagg tattttaaaa acgattgacg gtctttttga ctttaatctg atggccagat
     2101 tgagtttggg aaaagagaac tggaaaccac tgagtaagtc taaacgcaaa gaattttcta
     2161 agttgttcgt ggagcgtcta aaacagtcat atctggataa actagacctt tatactgatg
     2221 aagaggttgt ggttggagaa gcaaagcaga caaagaaaaa tcgtgttgaa gtattgactt
     2281 tcctggtcag caaggatgac aaaaaagaga tgacttacaa actctacaaa tcaaagaaaa
     2341 agggctggtt ggtttatgat gtggatgttc ttggagtcag cattgtggcg acttatcgat
     2401 cccagttttc aggtattttg aaaaagggat caatggaaca gttgttggaa cgaatgcgtt
     2461 caacaggaga actggggtca tgatgctaag gcagttttac gaaagagtca ttttaagctt
     2521 tcccaaaacc attttgcttt taatgctggt ttgcattgca gcattaggtt atcaagcacg
     2581 ctacctggaa attgatgctt cagcagaaac tttgttgct
//
LOCUS       DTVD01000072            2616 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16334_length_2616_cov_161.839906, whole genome
            shotgun sequence.
ACCESSION   DTVD01000072 DTVD01000000
VERSION     DTVD01000072.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16334_length_2616_cov_161.839906"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="EYO68_02585"
     CDS             complement(<1..684)
                     /locus_tag="EYO68_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06722.1"
                     /translation="MFVRIFTTVNQFPKTILLVVLALSAFFFVQARDGLFDPQTGRLR
                     INSTVEPFIERDSGAYQQFLDARKAFGSEEVVVIALHNTEKKPIGLEFLLTLAHLKSD
                     IETTVPGITKVFSMLDIPQASGECTGKSYFHQMGIGSVCFSVLEKYEQDISCLNSTAS
                     SSTQDTEGNLEESLEGGLEESLESNLEEGLESNLEESLEENLEASLEENTGDTTGLQS
                     ETETPEDTAP"
     gene            complement(692..2524)
                     /locus_tag="EYO68_02590"
     CDS             complement(692..2524)
                     /locus_tag="EYO68_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06723.1"
                     /translation="MCLLNHCSAQTEFPQTGSPDAVFPVHLTRKLVLSGILLSFLLSF
                     FFLFSVPSAFSQETSDPDFQESLEEDPLAETVPDLGLEEDPLAETSPDLGMEEDPLAG
                     TSPDLGMDEDPLAETVPDLDMEEDPLAETSPDLGMDEDPLAEESSQLGQEETLNFGES
                     EIQESETIVVEEEEYQPKVTFSHKVKTLIGGSETRGLSMLDPLLSEIKELRFVSTYDQ
                     SVKVQTSPRMYNSFRLSISFSQNYELNKQSIFDGFASLREIYSNYRTGPHQIRYGTQI
                     FDLGKVDLDKVIDVLHMNNVMGLYTFDPDDTKDSIPSIRYNWFRGAHTATMYFSPIRQ
                     QTFGMKFTEFREEVEKKEQDDEAGKVSFLRDYYGLQYQWTGDIIDARIGMFHWFDSNP
                     HIKFEYQRTADNETTSLQGSFEKMLSNYQEKETRSDFLTFELDAVWSDMVWKLESGLF
                     KKRNLYSYEIPEGKHIRLSTFRTPHFAWATSFERTFPYFYWLMIYSHRKSFDVPENSH
                     VFLYENESALVPQKRDVIRNQVSGVAVLKTPDNSLRITLLNYQTWPFIQRGFASLFTW
                     EEYKQNMELELRFFRLETERQKMLKNKITTNQVFLTYTQKFTAN"
BASE COUNT          733 a          566 c          525 g          792 t
ORIGIN      
        1 cggagccgtg tcctcaggcg tttctgtttc agactgtaac ccagttgtat cacctgtatt
       61 ttcttccaga cttgcctcca ggttttcttc caggctttcc tccaagttgc tttccaggcc
      121 ttcctccaag ttgctttcca ggctctcctc taatcctcct tccagacttt cctcgagatt
      181 accctctgta tcctgtgttg aagatgaagc cgtactattc aggcaggaaa tgtcttgttc
      241 atatttttcc aaaacagaaa aacaaacaga gccaataccc atttgatgaa aatacgattt
      301 tcctgtacat tcacctgaag cttgtggaat atccagcata ctgaagactt ttgtaatgcc
      361 gggaacggta gtttcaatgt ctgatttcaa gtgagccagc gtcagaagga attcaagccc
      421 tatcggcttt ttttctgtat tgtgcagagc aatcactacc acttcttcac tgccaaatgc
      481 ttttcttgca tcaagaaact gctggtaggc tcctgaatcc cgctcaatga aaggttcaac
      541 cgtgctgttg atgcgtaatc ttcctgtttg gggatcaaac aagccgtccc ttgcctgaac
      601 aaaaaagaag gcactcaggg caagaacgac caacaggatt gtttttggga attgatttac
      661 agtggtgaaa atgcggacaa acatggagga atcagtttgc agtaaatttt tgggtgtatg
      721 tcaaaaaaac ctgatttgtt gtaattttat ttttcaacat tttttgcctt tctgtttcca
      781 gtcggaaaaa cctaagttca agttccatgt tttgtttgta ttcctcccag gtgaaaagtg
      841 aggcaaaacc tctttgtata aagggccatg tctgataatt cagcagtgta attcgcagtg
      901 agttgtccgg tgttttcagg actgcaacac cagacacctg atttcgaatg acgtcccttt
      961 tttggggaac aagagctgac tcattttcat acaaaaaaac atgcgagttt tccggaacat
     1021 caaacgactt gcgatgtgaa tatatcatca gccagtaaaa ataaggaaaa gtcctctcaa
     1081 acgaagtagc ccaggcaaaa tggggagtcc tgaatgtaga aaggcgaata tgttttccct
     1141 ccggaatctc ataagaatac aagtttcttt tcttaaataa acctgattcc aatttccata
     1201 ccatatcact ccaaactgca tccagttcaa aagtcaggaa atcagaacga gtttctttct
     1261 cttggtagtt actcagcatt ttctcaaaag aaccctgcag agaagttgtc tcattgtctg
     1321 ctgttcgctg atattcaaac ttgatatgcg gatttgaatc aaaccagtga aacatgccga
     1381 tccttgcatc aataatatcc ccggtccatt gatattgcag accataataa tcgcgtagaa
     1441 atgacacttt gcctgcctca tcatcttgtt ctttcttttc tacttcttca cgaaactctg
     1501 taaatttcat gccaaatgtt tgctgccgaa ttggagagaa gtacatcgtt gccgtgtgtg
     1561 caccacgaaa ccagttgtat ctgatagagg gaatggaatc ttttgtatcg tccggatcaa
     1621 aagtgtatag ccccatgaca ttgttcatat gaagcacatc aataacttta tctaaatcaa
     1681 ctttacccag atcaaaaatc tgagttccat aacgaatctg atgaggacct gttcggtaat
     1741 ttgaatatat ttcccgtaaa ctggcaaagc cgtcaaaaat actctgttta ttcaactcat
     1801 agttttgcga aaagctgatg gaaaggcgga aagaattgta catccgggga gaagtttgca
     1861 ccttcacaga ctgatcataa gtagagacaa aacggagttc tttaatttcg ctcagcaacg
     1921 gatccaacat tgacaacccc ctagtttcac tgccacctat caatgttttt actttgtgac
     1981 tgaaagttac ttttggctga tattcttcct cttcaactac tatagtttcg ctctcctgta
     2041 tttcagattc accaaaattt agagtttctt cctgtcctag ctgggatgat tcttcggcaa
     2101 gtgggtcttc gtccatacca agatccgggc ttgtttcggc aagtggatct tcttccatgt
     2161 caagatctgg aactgtttcg gcaagtgggt cttcgtccat gccaagatcc gggcttgttc
     2221 cggcaagtgg atcttcttcc atgccaagat ccgggcttgt ttcggcaagt ggatcttctt
     2281 ccaaaccaag atctggaact gtttcggcaa gtggatcttc ttccaaactc tcctgaaaat
     2341 ctggatcgct ggtttcctgt gaaaatgcag atggaactga aaagaggaag aagaaggaaa
     2401 gtagaaaaga taacaaaatc ccagacaaga caagttttct ggtcaagtgg acaggaaaaa
     2461 ctgcatccgg agaaccagtt tggggaaatt cagtctgtgc tgaacagtga ttcaggaggc
     2521 acatttttga gatcaggatc ggcagataaa tcgagataca gtttcagttt tccattcttt
     2581 ttttgaatat gagtcagggt ttcaaaaatg aatttt
//
LOCUS       DTVD01000073           32389 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_163_length_32389_cov_301.814189, whole genome
            shotgun sequence.
ACCESSION   DTVD01000073 DTVD01000000
VERSION     DTVD01000073.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 32389)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32389)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32389
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163_length_32389_cov_301.814189"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            85..333
                     /gene="pheA"
                     /locus_tag="EYO68_02595"
     CDS             85..333
                     /gene="pheA"
                     /locus_tag="EYO68_02595"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIC06724.1"
                     /translation="MDKLRKRIDELDAELLKLLNERAECVVKIGAIKQKEESKVVVPQ
                     REKELLNRLTTLNNGPMTEKMVHRLFQEIIDTLKELQE"
     gene            complement(361..870)
                     /locus_tag="EYO68_02600"
     CDS             complement(361..870)
                     /locus_tag="EYO68_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIC06725.1"
                     /translation="MQGFNNRIFLLALAAIVGAAAFYINSKNGTVTSSTGENVPKTST
                     SNQEVIVTNTTSTDTITTASGLQYHVIAEGSGTESPGPDSVVSVHYRGKLTDGFEFDS
                     SYKRNQPATFPVNGVIRGWTEALQLMKVGDKWELTIPPDLGYGSQGAGSTIPPDATLI
                     FEVELLEIR"
     gene            complement(933..1835)
                     /locus_tag="EYO68_02605"
     CDS             complement(933..1835)
                     /locus_tag="EYO68_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HIC06726.1"
                     /translation="MFSNETKQIRQMLEKQNYVADSAIVMSVYLAKTLQKPLLVEGPA
                     GVGKTEIAKVMAKALDTDLIRLQCYEGLDANMALYEWNYQRQLLHIKLEEGSDKSLAE
                     RESTIFSEDFLLKRPLLQAITHHKPPVLLIDELDRSDEEFESFLLEILSEWQITIPEI
                     GSIRAKEKPYVILTSNRTRELSDAVRRRCFYLWMDYPDYEKELAIVRSKVPGIDLRLA
                     KQICIFMELIREQKLEKVPGVAETLDWARSLVMLHQDHLDPEMVKATLGIVFKDRMDI
                     EHIKDSLADFFDTVGVKIKEVPVK"
     gene            complement(1877..2305)
                     /locus_tag="EYO68_02610"
     CDS             complement(1877..2305)
                     /locus_tag="EYO68_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HIC06727.1"
                     /translation="MNTSSIYRDLLLDHARHPKNSGCLEHPDLKASAFNPLCGDELEL
                     TLALSGETIHDCRTQVRGCTICIASASMMSEFILERTLSEVLQISKEFSKSLRKDTED
                     IPETIESLRPLIDLKPHRSRIKCMRLAWDALEDCAAQHQT"
     gene            complement(2307..3560)
                     /gene="sufS"
                     /locus_tag="EYO68_02615"
     CDS             complement(2307..3560)
                     /gene="sufS"
                     /locus_tag="EYO68_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIC06728.1"
                     /translation="MQHHPLASKVRDDFPILKREINDFPLAYLDNAATTQKPQCVIDS
                     LLDFYSTHNSNVSRGVYTLAEEATEIYEEGRESIAKFIGATDSGNIIFTRGTTESINL
                     VASAWAQHNLKTGDRIIVTEMEHHSNLVPWQMAAQASGAELVYWPITPEAGSTGVGLL
                     ELEELEELLTDSVKLLAVTAVSNVLGTVNPIAKIVEMAHERGIPVLVDAAQAVARMPI
                     DVTGWDCDFLAFSGHKVYGPTGIGVLYAKKERLDEMQPFQGGGGMIRQVGRQSSTWAD
                     SPQKFEAGTPPVAEVSGLAAALEYVSDVGISEIQKHEKELTAHALNRFAEFSDLELYG
                     PQDTCDRTGVLSFNLRNVHPHDVAQVLDESGIAVRAGHHCTQILHERLGVAASVRMSF
                     GIYNEVSEIDHLFSTLDRARDIFLD"
     gene            complement(3665..4450)
                     /locus_tag="EYO68_02620"
     CDS             complement(3665..4450)
                     /locus_tag="EYO68_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIC06729.1"
                     /translation="MFNEYFEKLAEFNAAEVPFAVATVIKITGSVSAKPGAKSIIDSK
                     GQTVFGWVGGGCAEEAVREASLESMRDGQTRIVPLDLDDEILGVGMPCGGTMEVYVEP
                     YMPRPELMIVGHGRIAEVLAELAHTVHFSITVNDSGATRETYPMAERLITSDLDFSKM
                     EIGPQTYVVVVTQHKGDQHSIKKALEGHGPYIGLVASTKRAKLVFKYLLDEGVAPEEL
                     KRVHSPAGLDFAGTTPEEIALSIVGEMVTIRRGGSGKSMMELK"
     gene            complement(4554..5147)
                     /locus_tag="EYO68_02625"
     CDS             complement(4554..5147)
                     /locus_tag="EYO68_02625"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIC06730.1"
                     /translation="MISAIILAAGESRRMGVKNKLLLPISGEVLISNFVKSVCASNVD
                     EVVVVVGHEAEKIEDVLQGQPVRFVENLRYMEGMTSSIQTGIQAASAESEGLLICLAD
                     QPFIETSDFNRLIHEFTDLFDSESSLIIVPVFKGQRGNPVLFSRQFRDIILQHKGEGC
                     RDIVLKHPECVREVEMGNDNVLQDVDTPEDYKMFCTD"
     gene            complement(5185..5760)
                     /locus_tag="EYO68_02630"
     CDS             complement(5185..5760)
                     /locus_tag="EYO68_02630"
                     /inference="COORDINATES: protein motif:HMM:PF06240.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06731.1"
                     /translation="MKVKADKKFLVNADIDSVWTVLTNPEKIVVCVPGAQLTETIDED
                     HFKGKVTIKIGPVTAKFNGDVEFTQRDSSTYELSMEGKGADISGKGGATMNMELSLNA
                     HETSTEVSCIMTVSITGRIAQFGSRMIEAVNNKLFEQFIQNFSNLLTQENERGNVDEV
                     PSEAEPVKAASLVGSIIVSELKRKFGKKAEL"
     gene            complement(5763..6395)
                     /locus_tag="EYO68_02635"
     CDS             complement(5763..6395)
                     /locus_tag="EYO68_02635"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIC06732.1"
                     /translation="MPENLQVISHPLVQHKLSLMRKKSTSTQSFRTLIREVGTLLAYE
                     VTKDLPLIYEEIETPLMKMDAPFLEGKKLCLVSILRAGNGLLDGFMELLPSARVGHIG
                     LYRDPETLVAVEYYMKLPDLINQRLVLALDPMLATGHSSAAAVTRLKENGAENIRFVC
                     LLAAPEGIEYFNKVHPDVPIFVAAIDEKLNDHAYILPGLGDAGDRIFGTK"
     gene            complement(6508..8145)
                     /locus_tag="EYO68_02640"
     CDS             complement(6508..8145)
                     /locus_tag="EYO68_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06733.1"
                     /translation="MSTSNEVFTPEEQVILSSYVTNTDRSVFVLTNLPEVIKGALFSR
                     YSRSTLGLRTLLLRDFIQEKESRFSDMQTGSNQSDSAKKTELAIEYAQKFYDRILDGY
                     GDDSIGELGGAHLALENISILATKTVEDARIGGSPLEKSTRYVTFAEKITGLSGNEEF
                     RFYQEPTLLNSAHRDLYLETCRNLFETYVRFTEPLRTHVRKQLPRDPEISQAAYDRSV
                     RARGFDIIRGLLPSATLTNMGVFGNGRFFETLIMKLRTEPFGELNEIGQFAFEELAKV
                     IPSFVRRAEAGHRHFQDFRNFSLAQQKLISKFYSKYLGQLKADSAEAVRLVDFDPEAE
                     TKLLAALLYSHSGLTLQQIRERVRALPDSEKTRLIEETVALRNHRRHKPERGLEMPFY
                     TFDILGDYGMYRDLQRHRMLTQERQPLTTRFGYDTPYEIEDAGLGAEYHETMARSAEA
                     FETIAKDFPYEAQYVVPMSYNIRWYVHINLRALIWLTEIRSTPQGHTGYRRIAQEMFR
                     KVEAAQPLLAKYMKFVDLNEYSLGRLSAEQRQEDKQA"
     gene            complement(8155..8739)
                     /locus_tag="EYO68_02645"
     CDS             complement(8155..8739)
                     /locus_tag="EYO68_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HIC06734.1"
                     /translation="MELKVKQVQRVDGAKEALYLPAIFGGLRLTVSHFWRNLFGAKDV
                     VTVSYPEEKRHVSERWRGRHRLTRLDNGFLSCVACYMCETVCPSECIHIEAGEHPDLS
                     VEKYPVVFDIDELRCVFCGLCVEACPKDAIRMDTGVISMAYTSRHTFDFTRDLLRDSY
                     DPKSHSNTKYEDNDLPSPKDQTEALPNSLGVPVT"
     assembly_gap    8746..8845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8846..9153)
                     /locus_tag="EYO68_02650"
     CDS             complement(<8846..9153)
                     /locus_tag="EYO68_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008277204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIC06735.1"
                     /translation="MDLTFMRQGYQNEGLTKEQLSPDPFLQFEAWFKEANEAEPIPNA
                     MSLATVNHSGAPMMRTVLLKLFDDNGFVFFTNYKSRKAEQISENPNVAVLFNWVALER
                     "
     gene            complement(9188..10261)
                     /locus_tag="EYO68_02655"
     CDS             complement(9188..10261)
                     /locus_tag="EYO68_02655"
                     /inference="COORDINATES: protein motif:HMM:PF00146.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIC06736.1"
                     /translation="MEILLEIIMIPAVLAIIKIVVIVLFFAMPLGTFLTLMERKWSAM
                     IQDRVGPNRANIGNFTGHGIMHLAADGLKSIFKEDTMPKGADGFLYLIAPFFGLIAAV
                     ATFAIIPIAGPIGDFTFQVTDIEPGLLFIFAITSLSVYGAVLAGTSSNSKFALLGGTR
                     ASAQMISYEVFMGLALMGIFMVYGTTRVSGIVDGQNEYWFGSLIPMWGVFTQPLGFVL
                     FFTALVAETKRAPFDAPEGESEIVAGYFLEYSGMRWSAFMLAEYVALVGVASLITTLF
                     FGGYHIPWLHLWESAPQWLVTILQIIKFSVFTLFFCWFQIQLRWTVPKFRFDQTMALG
                     WKKLLPLSLINLFVTAFVILAFA"
     gene            complement(10297..11514)
                     /locus_tag="EYO68_02660"
     CDS             complement(10297..11514)
                     /locus_tag="EYO68_02660"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIC06737.1"
                     /translation="MTVLQSRSEDASTGEDFSASVMSIEIGPAHPAMHGIVRFTTHLD
                     GELIVGMDPEIGYLHRGFEKESENSKWIQVIPYTDRLNYVSPLLCNIGYSMAVEKLYG
                     IEAPRRAEFTRVIMGEISRITDHLTCLGAVAMEIGAFTFFLWFIKAREYLWELVEEMT
                     GARLTTNWTRFGGNAFDLPDGFLSDLSDRLEKLKEIMEECDNLLTRNKIFIDRVKDVG
                     AISKEDALSYGFTGPMLRACGIPYDVRRADSYSVYDELDFEIPVGSTGDCFDRFLVRM
                     EEIRQSISIIEQAADKMPEGPFMTDERMMHFPDKKDVYGNIEGLIDHFKLVMEGHQPP
                     AGEIYMPTEGGNGEIGFYLVSDGSGDPVRVRMRPPCFNFVAAMPAMTVGHYVADIVPI
                     FGSVNMIGGELDR"
     gene            complement(11520..>11738)
                     /locus_tag="EYO68_02665"
     CDS             complement(11520..>11738)
                     /locus_tag="EYO68_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIC06738.1"
                     /translation="SIHHLWKAVDWYERECFDMFGIVFEGHPDLRRILMYDEFEGHPL
                     RKDYPIDKQQPLVELKEVEERHVYGRHA"
     assembly_gap    11739..11838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11839..12456)
                     /locus_tag="EYO68_02670"
     CDS             complement(<11839..12456)
                     /locus_tag="EYO68_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIC06739.1"
                     /translation="METDYGGFKTTKFDSVVNWSRKFSLFQYPFVTACCGMEYMAAAC
                     SHYDISRFGAEIPRFSPRQADVLWVVGTITHKMTPILKTIYDQMADPKWVIAFGACAS
                     SGGFYQNYSVLQGIDRLIPVDLYIAGCPPRPEAVLDGLIELQNSIQNDGPTANGITPT
                     AIRHKDQKADQLNRYFEISKKEVFENLQNPKSNRISTPGFTYSEDF"
     gene            complement(12524..12691)
                     /locus_tag="EYO68_02675"
     CDS             complement(12524..12691)
                     /locus_tag="EYO68_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002800940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIC06740.1"
                     /translation="MPFKYREIERNLKKMGFQVVRQKGSHVIFSNGRDTFPVPKSTVQ
                     MIYLQEWNVSC"
     gene            complement(12681..12902)
                     /locus_tag="EYO68_02680"
     CDS             complement(12681..12902)
                     /locus_tag="EYO68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06741.1"
                     /translation="MGNMQTTIEKDGDGYLAKVKDQPNLFAFAYSEKEAVQELKNVVE
                     MIMDYHLEQINAERLIRNELTALQEQYAV"
     gene            complement(12975..13337)
                     /locus_tag="EYO68_02685"
     CDS             complement(12975..13337)
                     /locus_tag="EYO68_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIC06742.1"
                     /translation="MTIASGYLPILIMIVLGLILAGAFSCIGLFLGPKLRGEVKETPF
                     ESGLLRDGMAGHRYDVKFYMTALVFLIFDVEVVFLYPWAVQIRELGWFGFVASSVFLG
                     ILILGLAYDWKKGALEWE"
     gene            complement(13559..15484)
                     /gene="metG"
                     /locus_tag="EYO68_02690"
     CDS             complement(13559..15484)
                     /gene="metG"
                     /locus_tag="EYO68_02690"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIC06743.1"
                     /translation="MTDTFYITTPIYYVNSHPHIGHAYTTIVTDVLTRFRKLFGDDTY
                     FLTGTDEHGQKIVESAAKAGVEPQAFVDEISREFREMWPHLHIDFDQFIRTTDPEHKA
                     CVQQILQKIYDQGEIYLKEYEGLYCVGCERFLDEAELEDGNCPDHQTPPEFYQEQNYF
                     FKMSAYQDWLLEELKQNESWIYPGRYRNELIQFLKAPLQDLCISRPKSRLAWGIDLPF
                     DKNFVTYVWFDALLNYASALGWPEGEKYIKYWPHVHHTIGKDILKTHGIYWPCMLKAA
                     GLPVFKKLVVHGHWVVGGSKMSKSLGNVVDPLAMKDQLGVDALRYFLLRDMSFGEDSN
                     FTEDLAITRYNGDLANNFGNLLNRSISMSRNNFEGCVPPLGEVGELEKDLRNSFSKGV
                     ETCREYILAFQPHRALEHIAWLSSQVNKYIDNCKPWSLAGQPEDRERLGTILYTALDM
                     ARILVGLLDPVMPEKMCEARKSLGLVTEAIPFEKITPGVLVAGTELPEPTPLFPKMKI
                     SAKNESVVKDQLPEKIESKPAEKGKEWLAFDDFQKMELKVGHIRSCRKVEKSNKLLCS
                     EVDLAEGRMRSIVSGAAEFYTPEELTDRRVLVVANLKPVKLMGEVSEGMILFSDDNGK
                     LVLIEAPEDVNPGVTVR"
     gene            complement(15655..15948)
                     /gene="yhbY"
                     /locus_tag="EYO68_02695"
     CDS             complement(15655..15948)
                     /gene="yhbY"
                     /locus_tag="EYO68_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIC06744.1"
                     /translation="MTGKQKRYLRALAHPLKPLVNLGKHGLSPENKREIETLLLDHEL
                     IKLKVLDSCPLSKKECANAVGRIKGLEVVQVIGKTLVLFSPHPDDPEIKLPAA"
     gene            complement(15951..16244)
                     /gene="gatC"
                     /locus_tag="EYO68_02700"
     CDS             complement(15951..16244)
                     /gene="gatC"
                     /locus_tag="EYO68_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIC06745.1"
                     /translation="MTTKFSPEEVQKIARLSSLELTEKETEVFAEQFSGIMEYFELLK
                     TAEIPDVVTNVDESHIGDSRKDKMVKSPVSPEQFSPYLEDGFFKVPRVIDQEN"
     gene            complement(16241..16672)
                     /gene="fabZ"
                     /locus_tag="EYO68_02705"
     CDS             complement(16241..16672)
                     /gene="fabZ"
                     /locus_tag="EYO68_02705"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIC06746.1"
                     /translation="MNLEEIKNILPHRYPFLLVDRVLEVRDGEYCKALKNVSGNEEIF
                     LGHFPQQAVLPGVMIIEALAQTAGLVINSGKGETEAGAIVFFAGINNAKFRVPVVPGD
                     QLILETTLSNQRRNFWVFEGKASVDGKLAAQAEIKLMMQPT"
     gene            complement(16675..17277)
                     /locus_tag="EYO68_02710"
     CDS             complement(16675..17277)
                     /locus_tag="EYO68_02710"
                     /inference="COORDINATES: protein motif:HMM:PF03938.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HIC06747.1"
                     /translation="MVYDVELLNWSFTSSAGKIITSKNKVMKYLFAIFALASFSMFTQ
                     ASAFEIGVVDLNQALNQSEPGIRSKNILERRGRQKQQEFKLEESELRKLAEDLRNNPL
                     LTPKAKANKEQELIARQQQLREKVRSIEQEMRLEERRLTEVIFKELKTVIRSISIKEK
                     LDLVLEKNAAQVILYMKEDTTDLTQKVIDAYNELKQDGGS"
     gene            complement(17262..19163)
                     /gene="nuoL"
                     /locus_tag="EYO68_02715"
     CDS             complement(17262..19163)
                     /gene="nuoL"
                     /locus_tag="EYO68_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIC06748.1"
                     /translation="MSQIWLVPAFPLLGFILNGFLGKRFGTRFVTFVGPLAVGMAFAQ
                     SLVLFFQMLEAEGNVLNEHLYTWIASGNFEAGINFTVDQLSGLYLLIITGVGFLIHVY
                     SVGYMKGESGYYRFFAYLNLFVFFMLLLVLGDSFLLMFVGWEGVGLCSYLLIGYYFEK
                     DSAAEAAKKAFLFNRVGDFGVLSAVMLIFLSFGSIEFATVNAEASTRLTEGGTLVTAI
                     TLLLFLGATGKSAQIPLFVWLPDAMEGPTPVSALIHAATMVTAGLYMVARLSHLFVLA
                     PFTMNVIAVVGATTAILAATIAITQTDIKRVLAYSTVSQLGYMFLAMGVGAFGAGVFH
                     VMTHAFFKALLFLGSGSVILAIHHEQDMRKMGALKNKLPITYVTMLLGTLAISGIPFF
                     SGFFSKDEILWKAFSSPFGSPWLWFVGFLTAGMTAFYMFRMIYLTFHGESRVDSHVAE
                     HVRESPLSITLPLVILAALAVIGGFFGVPHVFHVIPNGIEVYFHDFFAEIPAGHGNVS
                     TEWTLMILSVIFALFTWFMASRLYHSGFEIASGLRSKWEWAYQLSLNKWYVDELYNSL
                     IIQPGRLLSTHLLWGLFDQNVIDRAVNTTGAVARSVGNTIRPLQNGLIQNYALIFTLG
                     TFLILWYMT"
     gene            complement(19166..19468)
                     /gene="nuoK"
                     /locus_tag="EYO68_02720"
     CDS             complement(19166..19468)
                     /gene="nuoK"
                     /locus_tag="EYO68_02720"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIC06749.1"
                     /translation="MIPIEHILLLSALLFIIGVLGVMLRRNVIVIFMSIELMLNAVNL
                     SFVTFARSLDSMTGVVFIFFIIVVAAAEAVVGLSIILSIFRTRKTLNIDEFNLLKW"
     gene            complement(19477..19989)
                     /locus_tag="EYO68_02725"
     CDS             complement(19477..19989)
                     /locus_tag="EYO68_02725"
                     /inference="COORDINATES: protein motif:HMM:PF00499.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIC06750.1"
                     /translation="MLTIFYIFAVLSVLGALGVLFLRNPIHCALSLVGTFFCLGAIYV
                     MLNAEFVAVIQVLVYAGAIMVLFLFVLMLLSSKDSEQYVNKWSVGKILAGLLSLGIFI
                     QIAGLFTANELQLGSKGEYPLEVVEEVGSITLIGRLLFTDYILSFEIIAILLLVAVIG
                     AVVIAKRRFQ"
     gene            complement(20024..21661)
                     /locus_tag="EYO68_02730"
     CDS             complement(20024..21661)
                     /locus_tag="EYO68_02730"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00384.20,HMM:PF10588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HIC06751.1"
                     /translation="MPVFQIDGEPVEYEPGEKVLSAALRCGKIIPHYCYHPGMSIVAT
                     CRMCLVDVIDLGNGRPAPKLQTACSVDATEGMKIETFNQKVEEGRKLVNEFLLINHPL
                     DCPICDQSGECTLQDYSFKYGSGKSEMDYSKRVNGWRDIGTFVALERNRCIQCSRCDR
                     FTREITGTNEFGMFNRGHELAVDTYTDRPMTNKFQGNMADICPVGAITEKEFRFKRRV
                     WKLKKNHSICTGCSTGCNVTIEHDRNEVFRLKPHENQNVNKWWLCDEGRLTYRKMNEK
                     KSRINQPLGLIGENLEGISWEKAYGAIAERIRELKPLPQEVLALTDTHASNEELFLIQ
                     KLLKEGFSSENIFCPFPKWEQSESDFFINTLITTDKTPNRAGALALHIKGDQKNKKLK
                     KVIEGELKVVFVLGNPFENELEFKEIIKRTQLVIHIGVFHNSWSEIADVVLPGQFYSE
                     KEGTYTNKNQRVQSTEIAVKALRRTRPEWQIISELSQALGHKSSFESIPQVFNVMVQE
                     AKAFTGISFDKIGGLGIELTKNTKDPGKKVVPEMTAI"
     gene            complement(21673..22932)
                     /gene="nuoF"
                     /locus_tag="EYO68_02735"
     CDS             complement(21673..22932)
                     /gene="nuoF"
                     /locus_tag="EYO68_02735"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIC06752.1"
                     /translation="MLEETLVLFKNIRNPKYDKSLAGYKTTGGFKSLKKVFGMKPEEV
                     VQEVKESGVRGRGGAGFPTGLKWSFMAKGTGKPSYLVANADESEPGTCKDRELMLKDP
                     HLFLEGLMIGSYAVGCHHAYVYVRGEFFPAIKTLNAAVDELYADGLLGHDPLGSGFNL
                     DITIHTGAGAYICGEETALLDSLEGKRGQPRVKPPFPAVSGFNGCPTSVNNVETLACL
                     PFIFSDGGVESFKSFGPQNNSGTHLVSLSGHVNSPGVYELRMDVSMKDIIYEIGGGIR
                     NGKKLKGVIPGGSSSPVMKADEIDVLYNFDDLVKIGSMMGSAAMMVFDEDTDVVKLLH
                     RITRFYAHESCGQCTPCREGTHWSRQIVRDFLNGNGSEEKLKRLDRVGGNMLGTTLCA
                     LGDAAALPIQSIVNKFPEEFRNYYATT"
     gene            complement(22935..23462)
                     /locus_tag="EYO68_02740"
     CDS             complement(22935..23462)
                     /locus_tag="EYO68_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIC06753.1"
                     /translation="MIELRLVLFLQCIEKRGFNLFSKDIQTKVDNLLSRYPIKQNALI
                     PILLLAQNENDGWLTEEWMQHVADICEVPLTHVEGVVTFYTMFRLKRPGKFHLQVCTC
                     VPCCLVGGTELLEHTEKKLNIHAGHTTTDGEFSIEEMECLGACSFAPAIIVNEEYHEQ
                     VTPDKMDKLIDQLKQ"
     gene            23773..25494
                     /gene="ilvB"
                     /locus_tag="EYO68_02745"
     CDS             23773..25494
                     /gene="ilvB"
                     /locus_tag="EYO68_02745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIC06754.1"
                     /translation="MKKRLNGSQILVEVLKKQGVEYIFGYPGGACMPIFDALVDAPEI
                     KIILVRHEQGATHMADGYAKATGKTGVVLVTSGPGATNTVTGILTAHMDSVPLVVLTG
                     QTITPNLGKDAFQEADVFGVTMPVVKHSYLVRDVKDLSRIVKEAFHLASTGRPGPVLV
                     DLPKDVVSEEWAEDFSAKMDLPGYSVPKSGNSNSIKKISELLAKSKRPLLYVGAGAVI
                     SSAGKQVKKLAEKLRAPVTTTLLGKGSISEMHQLSVGMLGMHGTAYANKAVIECDLIM
                     AIGARWDDRITGKVSEFCPNATKIHIDIDPAEFGKIIQPDISVNGDAALVLDELIPLV
                     KPLDTKEWLKKIENWKKKHPLKYPKRGGLRAQHVLAELDRLTGSNAIIATDVGQHQMW
                     AAQFCRSTRERQWLSSGGAGTMGYGFPAAIGAQLGNPEDLVVAVVGDGGFQMTMSELA
                     TAAINKLPLKILIINNSYLGMVRQWQQLFFDNRESGVDMEGNPDFVKIGEAYGIKGFH
                     LRRAGDVTKVLKKALAYNDGPCIIEAEVVKADNVFPMIPAGAAVSDMLIDEPPASKKL
                     KKPVGST"
     gene            25600..26091
                     /gene="ilvN"
                     /locus_tag="EYO68_02750"
     CDS             25600..26091
                     /gene="ilvN"
                     /locus_tag="EYO68_02750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIC06755.1"
                     /translation="MEEKFAISIYVCNKPGVLVRLAQTFARRGYNVDSLVVSPAHNTN
                     FSRITVVVQGVPSSYDQILRQLNKLVDVVHAASHPSTDIVDREMALFKVRAKPDQRAE
                     IFQIVEVFRAKTVDITDQSLVIETTGDSLKIDALEKLLEPMGLVEMVRSGKLIMKRGL
                     EET"
     gene            complement(26133..27077)
                     /locus_tag="EYO68_02755"
     CDS             complement(26133..27077)
                     /locus_tag="EYO68_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIC06756.1"
                     /translation="MNLRDLEYLSAIGELKHFRKAAEKCFVSQPTLSGQLKKLEDHIG
                     AQLVERTTRNVFLTPIGEEIVKISRRILADAASIENMVSTYTDPMSGTLNVGLIPTIA
                     PYLLPLIVQPINQAYPQLEIILQEVQTEVMLKKLSEGTLDAGILAVPLEMRGLEEIIL
                     YTEPFYVAANNQHPFAAKLSIKIEDLQDETLLLLEEGHCLRGQVMDVCSQAMTKERKD
                     FQGTSLETIRHMVSTGIGITLIPQTAIDYNNLIQNASIKYIPFKNPAPARRVGLLFRK
                     TSNRKVCFQKLAASIQSIIQKEFLLEETVEVLPVKLNR"
     gene            complement(27454..27630)
                     /locus_tag="EYO68_02760"
     CDS             complement(27454..27630)
                     /locus_tag="EYO68_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06757.1"
                     /translation="MSISNGDQMPEGSLKMMTDSVVKDKSTAELFNGRKVALFSVPGA
                     FTPTGSNKHLPSHL"
     gene            27944..>29204
                     /locus_tag="EYO68_02765"
     CDS             27944..>29204
                     /locus_tag="EYO68_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481639.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HIC06758.1"
                     /translation="MTTESKCPVMGKTHQAAGGTANQHWWPNQLNMRILRQNHPQDDP
                     NGEGFDYAKEFKSLDFEALTKDVDALMTQSQDWWPADYGHYGPLFIRLTWHAAGTYRI
                     TDGRGGGGAGAQRFAPLNSWPDNVNLDKARRLLWPIKQKYGQKISWADLLIFVGNRAL
                     ETMGFKTFGFGGGREDIWAPDEDTYWGPETVWLDDERYSGDRELAEPLGAVQMGLIYV
                     NPQGPNGNPDPLLAARDIRETFRRMAMNDEETVALIAGGHTFGKTHGAAEEHYKGPEP
                     EGAKIAEQGFGWTSSFGSGKGGDQIGSGLEGAWTTEPAKWDNGYYENLFDYEWELTKS
                     PAGAHQWTPKNSEAQGTVPDAHDSSKTHAPTMLTTDLALVTDPIYKEISKRFYENMDE
                     FADAFARAWYKLIHRDMGPAVRYLGPWV"
     assembly_gap    29205..29304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29305..30285
                     /locus_tag="EYO68_02770"
     CDS             <29305..30285
                     /locus_tag="EYO68_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007299134.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HIC06759.1"
                     /translation="KLIHRDMGPAVRYLGPWVPNEELLWQDPVPAVDHTLINDADIGS
                     LKAKILGSDLSISQLVSTAWASASSYRDTDKRGGANGARIRLSPQAEWDVNVASGIAP
                     VVATLEGIQQEFNNSQTGGKKVSLADLIVLGGCAAVQEAAKRAGQDVEVPFTPGRTDA
                     SQEQTDVESFAFLEPVADGFRNYLRAGHNLQTEHLLVDKAFTLNLTAPEMTVLVGGMR
                     VLNANAGASKHGVLTDRPETLTNDFFVNVLDISTEWKATSDAEQEFEGRDRKSGELRS
                     TGTRVDLVFGSNSELRSLAEVYASDDAQQKFVSDFVAAWDKVMNLDRFDL"
     gene            complement(30320..30553)
                     /locus_tag="EYO68_02775"
     CDS             complement(30320..30553)
                     /locus_tag="EYO68_02775"
                     /inference="COORDINATES: protein motif:HMM:PF13417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06760.1"
                     /translation="MELYFYEDCEYSQIVLNTISTLKIKDKFTFKDIRLNPDYAKELV
                     ELTGDVMVPCLITQDGPMKEAKDIRKYLVSHFL"
     gene            complement(30641..30937)
                     /locus_tag="EYO68_02780"
     CDS             complement(30641..30937)
                     /locus_tag="EYO68_02780"
                     /inference="COORDINATES: protein motif:HMM:PF08534.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIC06761.1"
                     /translation="MIDRFKEADTQVLGVSIDSKHSHKKWAESLGGVSYPLLQDFHPK
                     GAMSTSYGAYLENKGFAARSTVIVDKQGSVRYSVMADGERIIGELLAECKKVNS"
     gene            complement(30965..31099)
                     /locus_tag="EYO68_02785"
     CDS             complement(30965..31099)
                     /locus_tag="EYO68_02785"
                     /inference="COORDINATES: protein motif:HMM:PF00578.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIC06762.1"
                     /translation="MIAVGSEAPDFKLVSQFDSEYSLSEFKGEKNVMLVFYPLDWSAT
                     "
     gene            complement(31222..32265)
                     /gene="hflK"
                     /locus_tag="EYO68_02790"
     CDS             complement(31222..32265)
                     /gene="hflK"
                     /locus_tag="EYO68_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIC06763.1"
                     /translation="MGTWNKPPNKSPWEKGPQPPDIDELLSNLQDKFKIGLPKKGVFS
                     YIIILAIIVWLATGIFIIDPEEQGVIKRFGEVTEVVGPGPHYHLPSPIETVQIAPVTA
                     VRRLEIGFRTIQTGPPAKYRRVLKESLMLTGDENIIDVQFIVQYRISDLENYLYSLTN
                     PDVTVRSAAESAMREVIGDSTVTEALTVGKGIIEDTTALLLQQTMNSYKGGIKIENVK
                     LQDVHPPDAVKEAFKDVVSAREDREKMINDAEGYRNNLVPKARGEAAQLVNSAKGYAK
                     EKVLVAIGESERFNLIYEEYKMAKDITRERILLETMASILPKVNKVIADKELGRNVLP
                     FLPIGQSLNQLSK"
BASE COUNT         9055 a         7428 c         6585 g         9021 t
ORIGIN      
        1 atcatttttt tgaaaataaa atcaaataca ataaatacta agttttttac ggtagtcagt
       61 catagataaa ataatggaga taatttggac aaattaagaa aacgcattga cgaattggat
      121 gcagaattgt tgaagttgct caacgaacgt gcagaatgcg ttgttaaaat tggagcaata
      181 aagcaaaaag aagagtcgaa agtggtcgtc cctcagcggg aaaaagagtt gcttaatcgc
      241 ttaacaacgc taaataatgg gccaatgaca gagaaaatgg tacaccgtct ttttcaggaa
      301 atcattgata ctctcaaaga attacaggaa taatgtttca gttttgaggt ctgggccttg
      361 ttaacggatt tcaagaagtt caacctcaaa tataagtgta gcatccggag gaattgtact
      421 gccagcacct tgactcccgt aacccagatc cggtggaatt gtcagttccc acttatctcc
      481 caccttcatt aattgcaggg cttctgtcca tccgcgaata actccattaa caggaaatgt
      541 agcaggctgg tttcgtttgt aagaactgtc aaattcaaat ccatctgtaa gttttccacg
      601 ataatgcaca gaaacgacac tgtctggtcc aggactttct gttccggaac cttctgcaat
      661 tacatggtac tgcaatccac ttgcagtggt gatggtgtct gttgaagttg tatttgtcac
      721 tattacctct tgatttgaag ttgacgtttt tggaacattt tctccggttg aggaagtaac
      781 tgttccattc ttggaattta tataaaatgc tgcagctccc acaattgctg ccagtgcaag
      841 aagaaatatt ctgttgttaa atccttgcat agagcttctc agaaaaaata atgtcgaaga
      901 ataaaagaat tacttaaatt aaattcaata acttatttta caggaacttc tttaattttt
      961 acaccaaccg tatcaaaaaa atcagccagt gaatccttta tatgttctat atccatgcgg
     1021 tctttgaaaa ctataccaag tgttgctttc accatttccg gatccagatg gtcttgatgc
     1081 agcatgacta gtgacctggc ccaatccaaa gtttctgcaa ctccaggcac tttttccagc
     1141 ttttgctccc gaatcaattc cataaaaatg catatttgtt tggcgagacg cagatctatt
     1201 ccaggaactt tgctccgcac aatcgccagt tctttttcgt aatctggata atccatccac
     1261 aaataaaaac agcgtcggcg aacggcatcc gataattctc gggttcgatt gctggtcaaa
     1321 atcacataag gtttttcctt ggcacgtatg ctgccgattt ccggaatagt aatttgccat
     1381 tcagataata tttccagcaa aaaactttca aattcctcgt ctgagcggtc cagttcatca
     1441 attaataaaa ccggtggttt gtgatgcgta atcgcctgca ataatggacg tttaagtaaa
     1501 aaatcttcac tgaaaatagt ggactcccgt tcagccagtg atttgtcact accctcttca
     1561 agtttgatat ggagcaactg acgctgatag ttccattcat aaagcgccat atttgcgtca
     1621 agtccttcat aacattgaag ccgaatcaaa tctgtatcca gtgcttttgc cattactttt
     1681 gcgatttccg ttttacctac acctgcagga ccttcaacca ataatggttt ttggagagtt
     1741 tttgccaaat agactgacat tacgattgct gaatcagcca cgtaattttg tttctccagc
     1801 atttgccgga tttgttttgt ttcatttgaa aacataaatt attccgttat tagtcccgtg
     1861 cattcgttac ggcaactcaa gtttggtgtt gtgcagcaca atcttcaagc gcatcccacg
     1921 ccagtctcat gcacttgatg cggctccgat gcggtttcaa gtcaatcaac ggacgcagag
     1981 attctatagt ttcaggaata tcctcagtat cttttcggag gcttttgcta aactctttac
     2041 tgatctgcag tacttctgac agagttcttt ccaaaataaa ctcgctcatc attgaagccg
     2101 aagcaataca aatagtgcag ccccgtactt gtgtcctgca gtcatgaatg gtttctccgg
     2161 acaacgctaa agtcagttcc aactcatctc cacaaagcgg attgaaagcg gatgccttca
     2221 aatccggatg ctccaaacat cccgaatttt ttgggtgtcg tgcatgatcc agcaacagat
     2281 ccctgtaaat agaactagta ttcatatcaa tccaaaaaaa tatctctagc tcgatcaagt
     2341 gtgctaaaca agtggtcgat ttctgatact tcgttataaa tcccaaaact catgcgaacg
     2401 cttgccgcga ctccgagccg ctcatgcaga atttgcgtgc agtggtgtcc ggcacgtact
     2461 gcaattccac tctcgtccag aacttgcgcg acatcgtgag gatgcacatt ccggaggttg
     2521 aaactcaaca cgccagttcg gtcgcaggta tcctgaggtc cataaagttc cagatctgaa
     2581 aactctgcga acctgttcag cgcatgggca gtgagttctt tttcatgctt ttgaatttca
     2641 gaaattccga catcagatac atattccaag gctgccgcca atccggaaac ttcggccacg
     2701 ggaggagttc ccgcttcaaa tttttgcgga gaatccgccc atgtggaaga ttgccgcccg
     2761 acttggcgaa tcattccgcc gccgccttga aacggctgca tttcgtcgag gcgttctttt
     2821 tttgcgtaaa gcaccccgat tcctgttgga ccataaactt tgtgtccgga aaaagcgaga
     2881 aaatcgcaat cccaaccggt aacgtcaatc ggcatccgtg ccactgcctg agctgcatcc
     2941 accaaaaccg gaatgccgcg ttcgtgtgcc atttctacga ttttcgcaat gggattaact
     3001 gttcctaaaa cattggaaac tgcagttact gcaagcagtt taacagaatc tgtcagcaat
     3061 tcttccaact cttccaattc caaaagaccg acaccggtgc ttccggcttc cggagtgatt
     3121 ggccagtaca ccaattccgc tccggatgct tgtgcggcca tttgccacgg taccagattg
     3181 gaatggtgtt ccatttcggt cacgatgatc cggtctcccg tttttaaatt gtgttgcgcc
     3241 caagctgaag caacaagatt gattgactcc gtggttccgc gggtaaaaat tatatttccg
     3301 gaatccgttg cgccaataaa tttcgcaatg ctttcacggc cttcttcgta aatttccgtt
     3361 gcttcttcgg caagcgtgta aactccgcgg ctgacgttac tgttgtgagt tgaataaaag
     3421 tccaacagtg agtcgatcac gcattgcggc ttttgagtag tagctgcatt gtccagataa
     3481 gccagaggga agtcgttaat ttcacgtttg agaattggga aatcatctcg tacttttgat
     3541 gccaacggat ggtgttgcat ttttttgcgc taaaaaacaa ccacagattt gcgcggatta
     3601 gcacagaaat attatctgtt ttgtccgttt tgtctgtggc taatatcagg attcagggac
     3661 aaacttattt caattccatc attgattttc cggaaccgcc gcgacggatg gtcaccattt
     3721 caccgacaat gctcaaagct atttcttcag gagtcgttcc ggcaaaatcc aaacctgctg
     3781 gagaatgtac ccgctttaat tcttcaggag caacaccttc atcgagtaaa tacttaaaga
     3841 ccagttttgc ccgcttggtg ctggccacca aaccgatata cggcccatgt ccctccaagg
     3901 cctttttgat cgaatgctgg tcgcctttat gttgagtaac aaccacgaca taagtctgag
     3961 gtccaatttc cattttactg aaatccaaat ccgaagtgat caaacgctcg gccatcgggt
     4021 aagtttcacg ggtcgctccg gaatcgttga cagtgatgga aaaatgaacc gtgtgagcca
     4081 attctgccaa aacctcggcg atgcgtccgt gtcctacaat cattaattca ggacgtggca
     4141 tatacggttc tacataaact tccatggtgc ctccgcaggg catcccaacg cctaaaattt
     4201 catcatcgag atcaagggga acaatccggg tttggccgtc acgcatactt tccagagagg
     4261 cttcccgcac tgcttcctcc gcgcaaccac cgccgaccca gccgaatacg gtttgtccct
     4321 tgctgtcaat aatagatttc gctccgggtt tagcggaaac cgatccggta attttgatta
     4381 ctgttgccac cgcaaacgga acttccgccg cattaaattc tgctaatttt tcaaaatatt
     4441 cgttgaacat tttttagtcg ctttgcttct agtaaaatta taggtagtga ttcagcaaga
     4501 acttattgtt ctgcaatggc ttcctctgtt attgagtctc aaataatgta attttaatcc
     4561 gtacaaaaca tcttataatc ttccggagta tccacatcct gcaaaacgtt atcatttccc
     4621 atttcgactt cccggacaca ctccggatgt tttaatacaa tgtccctgca gccctcacct
     4681 ttgtgttgta aaataatgtc tctgaattgg cgggaaaaca aaaccggatt gccacgctgt
     4741 cctttaaaaa ctggaacaat aatcaaagaa ctttcagaat cgaataaatc agtaaactca
     4801 tgaattaaac gattaaaatc ggaggtttca atgaacggtt gatcagcaag gcatatcagc
     4861 aagccttcac tttccgcaga tgcagcctgt attccggttt gaatagagga tgtcatgccc
     4921 tccatgtagc gcagattttc tacaaaccgc accggttgcc cctgtaaaac gtcttcgatt
     4981 ttttctgctt cgtgaccaac cactaccact acttcatcta cgtttgaggc acagaccgat
     5041 ttcacaaagt tactgatcaa cacttccccg ctgattggca ggaggagctt gtttttcact
     5101 cccattctcc gggattcacc ggcggccaga atgattgctg aaatcattga agaaaaaaac
     5161 taaaaatgca ccgtaatgtt ttcattacaa ttctgctttt ttgccaaact ttcttttgag
     5221 ttcagacaca ataattgaac caacaagtga tgctgctttg accggttctg cttctgaagg
     5281 aacctcatcc acattgcctc tttcattctc ttgtgtaagc aaattggaaa aattctggat
     5341 gaattgttca aagagtttgt tatttacagc ttcaatcatg cgtgaaccaa actgagcgat
     5401 ccggcccgtg atggacacag tcataatgca cgaaacttct gtggatgttt catgcgcatt
     5461 tagagagagt tccatgttca tcgttgctcc tccttttccg ctgatgtctg ctccttttcc
     5521 ttccattgac agttcgtaag ttgaactgtc ccgttgagtg aattcaacgt cgccattaaa
     5581 tttggctgtc acaggaccga ttttaattgt tactttgcct ttgaagtgat cctcatcaat
     5641 ggtctcagtg agttgtgccc ccggaacaca aactactatt ttttcgggat tagtgaggac
     5701 tgtccaaact gaatcgatat ccgcattgac tagaaatttt ttgtcggctt taactttcat
     5761 ttttattttg taccaaaaat tctatctccg gcatctccta atccgggtaa aatataagca
     5821 tggtcgttca atttttcgtc aattgcagca acgaaaatag gcacatctgg atgtacttta
     5881 ttaaaatatt caatgccttc cggtgctgcc aacagacaga caaagcgaat attttctgca
     5941 ccattttcct ttaaacgtgt tactgccgca gcagaagaat gtccggtggc cagcattggg
     6001 tccagcgcca ataccaggcg ttgattgatt aaatccggaa gtttcatgta atattcaact
     6061 gcgaccagtg tttctggatc acggtacagc ccaatgtggc caactctcgc agatggaaga
     6121 agttccataa atccatccaa caatccatta cctgctctta atattgaaac cagacaaagc
     6181 tttttcccct ccagaaaagg agcatccatt ttcatcagtg gtgtttcgat ttcttcataa
     6241 atgagcggca agtccttggt cacttcgtaa gccagcaaag tgcctacctc ccggatcaat
     6301 gtacgaaaac tctgggtgga ggtgctcttt ttacgcatca gcgaaagttt gtgctgaaca
     6361 agtggatggc taatgacctg taaattttcc ggcattttta tctcctgtta attctatttg
     6421 accgttaatc atttatggtc tagtacttta cacttccaga ccgagaagag caaaccacaa
     6481 aatattagct aagtaagtgg gattgattta agcttgtttg tcctcttgac gctgttcagc
     6541 actcagacgg ccaagagaat actcgttcaa atcaacaaat ttcatatatt tcgccagtaa
     6601 aggttgtgct gcttccactt tccggaacat ttcctgggca atacgacgat atccggtgtg
     6661 tccttgtgga gtactgcgaa tttccgtcag ccatatcagg gcccgtaaat taatatgtac
     6721 ataccagcga atattgtagc tcattggcac cacatattgt gcttcatagg gaaaatcttt
     6781 agcaatcgtt tcaaaggctt cagcagaacg ggccatggtt tcatgatact ctgcacctaa
     6841 acctgcatct tcaatttcat acggtgtatc ataaccaaag cgtgtagtca gtggttgacg
     6901 ttcctgggtg agcatccggt gcctctgtaa atcacggtac atgccgtaat cccccaaaat
     6961 atcgaaagtg tagaaaggca tctccaaacc acgttccggt ttatgccgtc ggtgatttcg
     7021 caaggcaaca gtctcttcaa tcagtctggt tttttctgaa tccggtagag cgcggacacg
     7081 ctcacgtatt tgctgtaaag tcaatccgga gtgtgagtaa agcaaggctg ccagaagttt
     7141 ggtttccgct tccggatcaa aatctaccag tcgaactgct tccgcagagt ctgctttcaa
     7201 ttgccccaga tactttgagt aaaatttact aatgagtttt tgctgggcca gactgaaatt
     7261 tcggaaatcc tgaaaatgcc ggtgtccagc ttctgcacgc cgtacaaaag aaggaattac
     7321 ttttgctaat tcttcgaagg caaattgacc aatttcgttc agttctccga agggctcggt
     7381 tcttaatttc attatcaggg tttcaaaaaa acgcccgttg ccaaaaacac ccatgttcgt
     7441 cagagtagca gatggtaata atccacggat aatatcaaat ccacgtgccc tgacggaacg
     7501 atcgtatgcg gcttgactaa tctctggatc acgaggcaac tgtttccgga cgtgtgtccg
     7561 cagtggttca gtaaaacgga cataggtttc aaacaggttc cggcaagttt ccaaatacaa
     7621 atcacggtgc gcggaattta gtaaagttgg ttcttgataa aatcggaact cttcatttcc
     7681 ggacaatccg gttatttttt ctgcaaaagt tacgtagcga gtcgattttt ctaatggaga
     7741 gcctccgatt cgtgcatctt ccacagtttt ggtagctaaa atggaaatat tttccaacgc
     7801 cagatgcgct ccaccaagtt cgccaataga atcgtcacca tagccatcaa gtatcctgtc
     7861 ataaaatttt tgtgcatatt caatggccag ttccgtcttt tttgcagaat ccgattggtt
     7921 tgaaccagtt tgcatgtcag aaaatctgga ttctttttcc tgaataaaat ccctgagcaa
     7981 taatgtgcga agtccaagag tcgaacggga gtagcgggaa aaaagcgcac ctttgattac
     8041 ctcgggaaga ttagtgagta caaaaaccga gcggtctgta ttggtgacgt aagaagaaag
     8101 aatgacttgt tcttcggggg tgaaaacttc gttggaggtg ctcatgcaaa gattttatgt
     8161 caccggtact cccagggaat tgggcagagc ttctgtctgg tctttggggg agggtaggtc
     8221 attatcctca tacttagtat tggagtgaga ttttgggtca taactgtctc taagtaaatc
     8281 acgtgtaaaa tcaaatgtat gacgagaggt ataggccatt gaaatcactc cggtgtccat
     8341 tcgaatggcg tcttttgggc atgcttcaac acagagtccg caaaaaacgc agcgcagttc
     8401 gtcgatgtca aatactacag gatatttttc aactgaaagg tccggatgct caccagcctc
     8461 aatgtgaatg cattctgagg gacaaacggt ttcacacata taacaggcaa cacaactgag
     8521 aaatccgtta tcaagccgag tcaaccgatg tcgtccgcgc catcgttcgg aaacatggcg
     8581 tttttcttcc ggatagctga cagtcaccac atcttttgca ccaaaaaggt tgcgccagaa
     8641 atggcttacg gttagtctca gtccaccaaa aattgctgga agatacaaag cttcttttgc
     8701 accgtccacc cgttgaacct gtttaacttt tagttccatg tttccnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnntgacg ttccaaagcc acccaattaa aaagcaccgc
     8881 aacatttggg ttctctgaaa tttgttcggc cttcctgctt ttataattag tgaaaaatac
     8941 aaaaccatta tcatcaaaca gcttcagcaa aacagttctc atcatcggtg ctccggaatg
     9001 attgactgtt gcgagactca tggcattcgg tattggttcc gcttcattcg cttccttaaa
     9061 ccatgcttca aattgcaaaa acggatcagg acttaattgc tctttggtca gtccttcatt
     9121 ctggtagccc tgacgcatga aagttaaatc cattaattac tcctaaaccc ttgcttttcg
     9181 ggaatcatca cgcaaaagct agaatcacaa atgcagttac gaacaggtta attaatgaaa
     9241 gcggcaaaag ttttttccag ccgagagcca tcgtttggtc aaagcgaaat ttcggcactg
     9301 tccagcgtaa ctgtatttgg aaccagcaga aaaatagagt gaagaccgaa aacttgataa
     9361 tttgcaggat agtcaccagc cactgtggag cactttccca aaggtgtagc caggggatat
     9421 gatacccacc aaaaaataga gtcgtaatca aggaagctac accaacgagt gccacatatt
     9481 ccgcaagcat aaaagcagac cagcgcattc cggaatattc gagaaagtat ccggcaacga
     9541 tctcagattc gccttccggt gcatcaaacg gggctcgttt agtctctgca acaagtgcag
     9601 taaaaaagag aacaaatcca agtggttgcg taaaaacacc ccacatggga atcaggcttc
     9661 caaaccagta ttcattttgt ccgtccacaa ttccgcttac tcgggtagtt ccgtaaacca
     9721 tgaaaatacc catcaatgcc aaacccatga aaacttcata agaaatcatt tgagccgagg
     9781 cacgagtacc cccaagtagt gcaaacttac tgtttgaact tgttcctgcg agcactgctc
     9841 cgtatacact gagtgaggtg atggcaaaaa taaaaagcag cccgggttcg atatctgtca
     9901 cttgaaaagt aaaatcccca atgggtcctg caatcggaat gatggcaaat gtagcgactg
     9961 ctgcaatcaa accaaaaaaa ggtgcaatca ggtacaaaaa cccgtcagcc ccttttggca
    10021 tcgtatcttc cttgaaaatg gatttgaggc catctgcagc aagatgcatg attccatgtc
    10081 cggtaaaatt tccaatgttc gccctgtttg gtcctacgcg gtcctgaatc atggcactcc
    10141 atttacgttc cattagagtc aaaaaagtac ccaacggcat ggcaaaaaac agaacaatca
    10201 ccacaatttt aattatggcc agaactgccg gaatcataat tatttcaagt aatatctcca
    10261 ttttcgtttt tcttaactat tttcaataag ctggacttag cggtccaact ctccgccaat
    10321 catgttaaca gaaccaaaaa tcggtacgat gtctgcgaca taatggccaa ctgtcatggc
    10381 cggcatggct gcaacaaaat taaaacaagg cgggcgcatt cgtaccctca ctggatcacc
    10441 actaccatct gaaacgagat aaaaaccgat ttctccgttt ccgccttctg tcggcatgta
    10501 tatttctcct gcaggaggct gatgtccttc catgaccagc ttaaaatgat caatcagtcc
    10561 ttcgatattt ccataaacat ctttcttatc cggaaaatgc atcattcgtt cgtctgtcat
    10621 aaacggccct tccggcattt tgtctgcagc ctgttcaatt atgctgatgc tttgtctgat
    10681 ttcttccatc cggaccaaaa agcgatcaaa acaatcacct gtgcttccta ccggaatttc
    10741 aaaatcaagt tcatcataaa cagaatacga atccgccctc cggacatcat aaggtatacc
    10801 gcaagcccgc aacatcggtc cggtaaagcc ataggaaagt gcatcttctt ttgatatagc
    10861 tccaacatct tttacccggt caatgaaaat tttatttcgt gtcaggagat tgtcacattc
    10921 ctccatgatc tctttcaatt tctctaatcg atctgacagg tcgctcaaaa atccgtccgg
    10981 aaggtcaaag gcatttccac cgaaacgagt ccaatttgtc gtcaaacgcg cgccggtcat
    11041 ttcctctaca agttcccaaa ggtattcacg tgccttgata aaccagagaa aaaatgtaaa
    11101 tgcaccaatc tccatggcta ctgctcccag gcaagtcaga tggtctgtga tccgtgatat
    11161 ttcacccatg attactcttg taaattccgc acgtcttgga gcttctattc catacagttt
    11221 ttccactgcc attgaataac cgatattaca cagcagggga gatacgtaat tcagccggtc
    11281 agtatacggg atgacctgaa tccattttga attttctgat tccttttcaa aaccacgatg
    11341 cagatagccg atttccggat ccattccgac tatcaattct ccatccagat gtgtggtgaa
    11401 gcgcacaatt ccgtgcatgg ccgggtgagc tgggccaatc tcaatgctca taactgaggc
    11461 ggaaaagtct tctccggttg aagcatcctc agaacgggac tgaagtacgg tcatatctgt
    11521 catgcgtgcc tcccgtatac gtgtcgttcc tcaacttctt ttagttccac taaaggctgc
    11581 tgtttatcaa ttggatagtc tttacggagc ggatgtcctt caaattcatc gtacatcaaa
    11641 atccgtctca agtccggatg tccctcgaaa acaatcccga acatatcaaa acattctcgc
    11701 tcataccagt ctactgcttt ccacaaatga tgaattgann nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnga aatcttcaga atacgtgaat ccaggtgtgc tgatgcggtt
    11881 acttttagga ttttgtaaat tttcaaagac ttcttttttg gatatttcaa aatatcgatt
    11941 tagctgatct gctttttgat ctttatgcct gatggcagtt ggagtaatgc cgttggcagt
    12001 cgggccatca ttctgaatgc tattctgcaa ctcaataagt ccgtccagca cagcttcggg
    12061 tcgcggagga cagcctgcaa tataaagatc aaccggaatc aaacggtcaa ttccctgaag
    12121 tacagagtaa ttttgataga atcctccgga agatgcacat gcgccaaaag caatcaccca
    12181 ctttggatct gccatctgat cataaatcgt cttgaggatc ggtgtcattt tatgagtgat
    12241 tgttcctact acccagagaa cgtccgcctg tctaggagaa aatctgggaa tttctgcacc
    12301 aaaacgggaa atgtcataat gtgagcaggc tgcggccatg tactccattc cgcagcaagc
    12361 agttacaaac ggatactgaa aaagtgaaaa tttcctggac caattgacca cactgtcaaa
    12421 ttttgtggtt ttaaaaccac cataatctgt ttccataatt agttcctgtt aagttagcat
    12481 tatttgatat ttctaaattc ctctcttgtc attgacagaa tttttaacaa ctgacgttcc
    12541 actcctggag atatatcatt tgaaccgtgc tttttggtac cggaaaggtg tcccttccat
    12601 ttgagaaaat aacatgagaa cctttttgac gaacaacttg aaatcccatt ttttttaggt
    12661 ttctctcaat ttccctgtat ttaaacggca tattgttctt gtaatgcagt caactcattc
    12721 ctaatgagac gttctgcatt aatttgttca agatgatagt ccattatcat ttcaacaaca
    12781 tttttcagtt cttgaacagc ttccttctct gagtaagcaa atgcaaaaag attgggctga
    12841 tctttcactt tagccagata accatctcca tctttttcaa tagttgtttg catattgccc
    12901 atttttcacg aaattattct tttaacgctg gtcacagctg cattttgagg ttaaagaaaa
    12961 aacaatttaa tgaattactc ccattccagc gcaccttttt tccaatcgta agccaaacca
    13021 agaatcagta ttcccaaaaa gactgagctg gccacaaaac caaaccaccc taattcacga
    13081 atctgcactg cccagggata aagaaatacc acctctacgt caaaaattag aaagaccaaa
    13141 gcagtcatat aaaatttaac atcatatcga tgtcctgcca ttccatctct aagcaaacct
    13201 gattcaaagg gggtctcctt aacttcccct ctaagttttg gtcccagaaa caggccgata
    13261 cagctaaacg cacctgccag aatgagtccc agcacaatca taatcaatat cggcaggtag
    13321 cctgatgcaa tggtcataag agtaaatagt tttacaggtt tcaggttttt tgagaacagt
    13381 cggttttttg tctatgattt cctttacccc ctcttttcaa aatatactgc taatacagga
    13441 gtaaaaaggg aaaggttaaa acccgactgt tgcaccgtat cagcaccaaa aaatagatcc
    13501 ttcagctcaa aattagtgtc tcacacaaat cgcaaattgt aaatacattt caattgtttt
    13561 aacgtacagt cacacccgga ttcacgtctt ccggagcttc aatcaaaacg agttttccat
    13621 tatcgtcaga aaaaagaatc atgccttcag aaacttcgcc catcaatttg accggtttca
    13681 agtttgcaac gacaagaact ctccgatcag tcaattcttc aggagtgtag aattcagctg
    13741 ctccggaaac tatagaacgc atacgtcctt ctgctaaatc gacttcagaa cagagcagct
    13801 tatttgattt ttccaccttc cgacaagatc tgatatggcc aacttttagt tccattttct
    13861 gaaaatcgtc aaaagccagc cactcttttc ccttctccgc cggcttactt tctatttttt
    13921 ctggcagttg atcttttaca acagattcat ttttggcaga aattttcatt tttggaaaca
    13981 acggtgttgg ttccggcagt tccgttcccg ctaccaatac tccgggagta attttctcaa
    14041 aaggaattgc ctctgtaaca agtccgagag attttcgagc ctcgcacatc ttttccggca
    14101 ttactggatc cagcaagcca accaggattc ttgccatatc cagtgcagta taaaggatag
    14161 ttcccaagcg ctccctgtct tccggttgcc ctgccaggct ccatggcttg cagttgtcaa
    14221 tgtatttgtt gacctggctg ctgagccacg ctatgtgttc cagagcacga tgcggctgaa
    14281 aagcaagaat gtattcccgg cacgtttcca ctcctttgct aaacgaattc cggagatctt
    14341 tttccagttc tccaacttca cccaaaggag gtacgcagcc ctcaaaatta tttcggctca
    14401 tacttatcga gcggttgagt aaatttccaa agttattggc taaatcaccg ttgtaacgtg
    14461 tgatagccaa atcttcagta aaatttgaat cctcaccaaa actcatgtca cgcaataaaa
    14521 aataacgtag ggcatccaca ccaagttgat ctttcatggc cagtggatca accacattac
    14581 caagagactt actcatcttg ctgcctccta cgacccagtg accgtgaacc acaagttttt
    14641 taaagacagg caaacctgca gccttgagca tacacggcca gtatattcca tgcgttttta
    14701 aaatatcctt gcctattgtg tggtgtacat gcggccagta ctttatatac ttttctcctt
    14761 ccggccagcc taaggcgctc gcataattaa gcagcgcatc aaaccagaca taggtgacaa
    14821 agtttttatc aaaaggtaga tcaatacccc aggctaaccg gcttttaggg cgagaaatac
    14881 acaaatcctg aagaggtgct tttagaaact gtattagctc attgcggtaa cgtccgggat
    14941 atatccagct ttcattttgc ttcaactctt caagcagcca atcctggtaa gcactcattt
    15001 tgaagaaata attttgttcc tgataaaatt caggtggtgt ttgatggtcc ggacaatttc
    15061 cgtcctccaa ctctgcttcg tccaaaaaac gttcgcaacc gacgcaataa agaccttcgt
    15121 attcttttag ataaatttca ccctgatcat aaattttttg caggatttgc tgaacacagg
    15181 ctttgtgttc aggatcagtc gtgcgaataa actgatcaaa atcaatatgc agatgcggcc
    15241 acatttcacg aaattcccgg ctgatctcgt ccacaaatgc ttgcggctca acaccagctt
    15301 tggcggcaga ttcaactatt ttttgtccat gctcatcggt gccggttaaa aaataagtgt
    15361 catcaccaaa cagtttacga aagcgtgtca atacatcagt cacaatcgta gtgtaagcat
    15421 gaccaatgtg tggatggctg tttacatagt atattggagt ggtaatgtaa aaggtgtcgg
    15481 tcatacgagt tgagcaacta tgaattattt ctacaaagtc aaaaaacacc tctaaagatt
    15541 atttttcgac agatcggcga ggagtgtttt aagtattagg actgcacatt tcctgtgaaa
    15601 tacagccgtc attaaatata aattactttt tacaaatata gtttcagctg gagatcatgc
    15661 agcaggaagt ttaatttccg ggtcatctgg atgaggacta aaaagaacaa gtgtttttcc
    15721 aataacctgc acaacttcaa gacccttaat tctgcccacc gcattggcac attctttttt
    15781 tgataaaggg caactgtcaa gaacttttag tttaatcaat tcatggtcca gcagcagggt
    15841 ttcaatttct cgcttgtttt caggtgaaag tccatgcttg ccaagattca ctaacggttt
    15901 taacggatgg gccaatgcac gtaaatatct tttttgtttt cctgtcatgt ttaattttcc
    15961 tgatctatca ccctcggtac tttgaaaaat ccatcttcaa gatacggact aaactgttca
    16021 ggagaaactg gtgactttac cattttatct ttccggctgt ctcctatatg gctttcatct
    16081 acattggtaa caacgtccgg aatttcggca gttttcaaca attcaaagta ttccataatc
    16141 ccagaaaact gttctgcaaa aacttccgtt tctttttctg ttaattccaa agaagaaagt
    16201 cgggcaatct tttgtacttc ttccgggctg aatttagttg tcatgttggt tgcatcatta
    16261 atttaatttc tgcctgtgcc gccaatttgc catcgacact tgctttgcct tcaaagaccc
    16321 aaaagtttcg acgctgattg ctcaaagttg tttccaatat caactgatca ccaggaacaa
    16381 caggaacccg aaatttggca ttatttattc ctgcaaaaaa aacaattgca ccagcttcag
    16441 tctctccctt tccggaattg attacaagtc cggctgtttg cgccaaagct tcgatgatca
    16501 tcacaccggg cagcactgcc tgttggggga aatgccccag aaaaatttct tcgtttccgg
    16561 aaacattttt cagcgcttta cagtattctc catcacgaac ttccagcacc cggtctacca
    16621 gtaaaaaagg gtagcggtgc ggcagaatat tcttgatttc ctccaaattc atcatcaact
    16681 tcctccatct tgttttagtt cattgtatgc atcaatcact ttttgagtaa gatcagttgt
    16741 gtcttccttc atatacaaaa ttacctgtgc agcatttttt tcaagaacca aatcaagttt
    16801 ctccttgatg gaaatactgc ggatcacagt tttgagttct ttgaaaataa cttccgttaa
    16861 gcgtcgttct tccagcctca tttcctgttc aatggagcgg actttttcac gcagttgctg
    16921 ctgtcgtgca atcaattcct gttctttatt tgccttggct ttgggagtca acaacggatt
    16981 gttccgcagg tcctcagcaa gttttcggag ttctgactct tcaagtttaa attcctgctg
    17041 cttttgacgt cctcgacgct ccagtatatt tttggaacga attccaggtt cagactggtt
    17101 aagagcttgg ttcaggtcaa caactccaat ctcaaaggca gaggcttgag tgaacatgga
    17161 aaaagaagca agagcaaaaa tagcaaataa atatttcatg actttgtttt tactagtgat
    17221 tatctttcca gcagaagaag taaaactcca attaagcagt tctacgtcat ataccataga
    17281 atcaggaaag ttccaagggt aaaaatgagg gcataattct gtatcaatcc attctggagg
    17341 gggcgaatag tgttgccaac cgaacgtgca actgcaccgg ttgtgttgac agcccggtca
    17401 atcacgtttt gatcaaacag gccccaaagt aagtgggtcg atagtaaacg tcccggctga
    17461 atgattaacg aattatacaa ttcatcaaca taccatttat taagagaaag ttgatatgcc
    17521 cattcccatt tgcttctcaa tccggaagca atttcaaatc ctgaatgata caaacgactt
    17581 gccataaacc aggtaaaaag agcaaatatt acagacagaa tcatcagcgt ccactcagtc
    17641 gaaacatttc catgacctgc tggaatttct gcaaagaaat catgaaagta aacttctatt
    17701 ccattgggaa taacatgaaa cacatgtgga accccaaaaa aaccacctat aactgcaagt
    17761 gccgctaaaa taaccagcgg taaagttatt gacaaagggg actcccgtac atgttcagca
    17821 acatgtgaat ccactcttga ctctccgtga aatgtcaaat agatcatccg gaacatataa
    17881 aatgctgtca tgccggcagt caaaaatcct acaaaccaaa gccacgggct tccaaatgga
    17941 gaactgaagg ctttccagag aatttcgtct ttgctgaaaa atccggaaaa aaaaggaata
    18001 ccggaaatag ccaatgttcc gagcagcatg gttacatagg tgatcggcag cttgtttttc
    18061 agtgcaccca ttttccgcat atcctgctca tggtgaattg ccagaatgac ggaaccggaa
    18121 cccaaaaaaa gtagtgcctt aaaaaaagcg tgtgtcatta cgtggaaaac tcctgcgcca
    18181 aaagctccga ctcccattgc cagaaacatg taacccaact gactgaccgt tgaataagcc
    18241 aggacccgtt tgatatcagt ttgcgtaatg gcgatggttg ccgccaaaat ggcagtggtt
    18301 gccccaacga cagcaatgac gttcattgta aaaggagcca gtacaaacaa gtgacttaag
    18361 cgggcaacca tgtacaggcc ggcagttacc atagttgccg catggatcaa cgcagaaacc
    18421 ggagtaggcc cttccatggc gtctggaagc cagacgaaaa gagggatttg cgctgatttt
    18481 ccggttgctc caagaaagag cagcaaagta attgccgtga ccaatgttcc accttcagtg
    18541 aggcgggtgg aagcttctgc attgactgtg gcaaattcga tggaaccaaa tgacaggaaa
    18601 atcaacatta ctgctgacaa cacgccaaaa tcaccgactc gattgaaaag gaaagctttt
    18661 ttggcagctt cggcggcaga atctttttcg aaataataac ctatcaacaa ataggaacaa
    18721 agaccaactc cttcccagcc cacgaacatc agcaaaaagc tgtcaccgag aaccagcagc
    18781 agcatgaaaa acacaaataa attcaggtag gcaaaaaagc ggtagtagcc ggactctcct
    18841 ttcatgtatc ccacagaata tacatgaatc aaaaaaccga ctccggtgat tatgagtaaa
    18901 tacagtcctg aaagttgatc gactgtaaaa tttatccctg cttcaaaatt tcctgaggca
    18961 atccaggtat aaaggtgttc attcagaaca ttcccctctg cctcaagcat ctgaaaaaac
    19021 agaactagcg actgggcaaa tgccattccg actgccagtg gaccgacaaa ggtaacaaaa
    19081 cgtgtaccaa aacgttttcc caaaaaaccg tttaggataa atcccagcag ggggaatgcc
    19141 ggtacaagcc aaatttgtga catagctacc actttaataa attaaactca tcaatattca
    19201 gagtttttcg tgttcgaaat attgaaagaa ttatcgataa gccgaccacc gcttctgcag
    19261 cggcaaccac aatgataaaa aagataaaga caacaccggt catggagtcc aagctcctgg
    19321 cgaaagtcac aaatgacaag ttgactgcgt tgagcatcag ttcaatgctc ataaatatca
    19381 caatgacgtt gcggcgcagc atcacaccca gtaccccaat tataaaaagg agtgcactca
    19441 acaataaaat atgttcaatg ggaatcataa aaagacttac tgaaaacgtc gttttgcaat
    19501 cacaaccgcc ccaattaccg caactagcag gagaatagct attatttcaa atgacaaaat
    19561 ataatctgta aaaagcaggc gtccaattag agtaatcgaa ccaacttctt ctacaacctc
    19621 aagcggatat tcccccttcg atccaagctg taattcgttt gctgtgaata gaccggcaat
    19681 ttgaataaaa attcccaatg agagaagccc agccaaaatt tttcccacgg accatttgtt
    19741 gacatactgt tcagagtcct ttgaggaaag cagcatcaac acaaagagga aaagaaccat
    19801 gattgcgcca gcataaacca aaacctgaat gactgctaca aactctgcgt tgagcattac
    19861 atagattgca cctaaacaaa aaaacgtgcc caccaaagac agcgcacagt gtattggatt
    19921 ccgtaaaaaa aggacaccca gcgcacccag cacgctcagt acagcaaaaa tgtaaaaaat
    19981 tgttaacata tctaataatt acggaatgaa taatggtgga ctattaaata gctgtcattt
    20041 ccggtacaac ttttttacca gggtcttttg tatttttcgt cagttcaatt ccaaggcctc
    20101 caattttatc aaaactaatt ccggtaaatg cctttgcctc ctgaaccatt acgttaaaaa
    20161 cttgtggaat actttcgaaa ctacttttgt gccccagagc ctgcgataat tctgaaataa
    20221 tctgccactc cgggcgagtc ctgcgtaaag ccttgacggc aatttcagtc gactgaacac
    20281 gctgattttt gttggtatag gttccttctt tttctgaata aaactgtccg ggaagaacga
    20341 catcagcaat ttcactccag gaattgtgga aaactccaat atgaattacc agctgtgttc
    20401 gctttattat ttccttaaac tccaattcgt tttcaaatgg gttgcccagt acgaagacta
    20461 ctttcaattc accttcgatc acttttttaa gttttttgtt cttctggtct cccttgatat
    20521 gaagcgccag ggctccagcc cggttgggag ttttgtctgt agtgatgagg gtgttgatga
    20581 aaaaatcact ttcactttgt tcccatttcg gaaagggaca aaagatgttt tcagaagaga
    20641 atccttcttt cagaagtttt tgaattaaaa ataattcttc gttgctcgca tgagtgtcgg
    20701 taagtgcaag aacctcctgg ggaagcggct tcagctctct gattcgttcc gcaattgcac
    20761 cgtatgcttt ttcccatgaa ataccttcga gattctctcc aattagtccc aaaggctgat
    20821 ttattctgga ttttttttca ttcatttttc tgtaagtcaa acgaccttca tcacacaacc
    20881 accatttatt gacattttgg ttttcgtgag gtttgagtcg aaacacttcg ttacggtcat
    20941 gttctatcgt aacattgcag cccgtagagc agccggtaca aatggaatga ttttttttca
    21001 gtttccaaac gcgacgcttg aagcggaact ccttttccgt gatggcgccg accggacaaa
    21061 tgtccgccat gtttccctga aatttattcg tcatcggtct gtctgtatag gtatccacgg
    21121 ccaattcatg tccacggtta aacatgccga attcgttggt accggtgatt tccctggtaa
    21181 atctgtcaca gcgagaacat tgtatgcaac ggttacgttc aagggctaca aaagtaccaa
    21241 tgtcgcgcca gccgttaacc cgttttgaat aatccatttc cgatttaccg gaaccatatt
    21301 taaaggaata atcctgcagt gtacattctc cggactgatc acagatcgga caatcgagag
    21361 ggtgattaat cagcaaaaat tcgttaacaa gtttccgacc ctcttcaact ttttgattga
    21421 aagtttcgat cttcatgcct tccgtggcat cgacagaaca agcggtttgg agttttggtg
    21481 caggtctgcc attaccaaga tcgatcacat ccactagaca cattcggcag gtggcgacta
    21541 tagacattcc gggatggtag caataatgag gaattatttt tccgcaacgt aaagcggcag
    21601 aaaggacttt ttcgcccggc tcatattcaa ccggctcacc atcaatttga aatacaggca
    21661 tggaaaactc gtttaagtag tggcataata attccggaac tcctccggaa atttgtttac
    21721 aatactttga atcggcaacg cggcggcatc ccccaaagca caaagagtgg tccccaacat
    21781 gtttccgccg actcggtcaa gacgcttcaa tttttcttca cttccgtttc cattgagaaa
    21841 atcccggaca atttgacgag accagtgagt tccttcgcgg cagggcgtac actgtccgca
    21901 ggattcatgg gcgtaaaaac gggtaatgcg gtggagtaat ttcactacat cagtgtcttc
    21961 atcaaagacc atcattgccg cagaccccat catggaccct atttttacca aatcatcaaa
    22021 gttataaagc acatcaattt catcggcttt catgacaggt gaagaggagc ctccgggaat
    22081 tacaccttta agcttctttc cattccgaat gcctccgccg atttcgtaaa taatgtcttt
    22141 catactgaca tccatccgca actcataaac tcccggacta tttacatgac cactcaagct
    22201 cactaaatgc gtccctgaat tattttgtgg accaaaactt ttgaaagatt ccactcctcc
    22261 atcgctaaaa ataaagggca aacaggccaa tgtttcaaca ttgtttacac tcgttgggca
    22321 tccgttgaag cctgaaactg cagggaaagg aggttttact ctgggctgtc cgcgtttgcc
    22381 ttccaatgaa tccagcagtg cggtttcctc accgcaaata taagcacctg cgccggtgtg
    22441 gatagtgata tccaaattaa agccgctgcc cagcgggtcg tgtccaagaa gcccgtctgc
    22501 atataattca tcaacagcag catttaaggt cttgatggct ggaaaaaatt caccacgaac
    22561 atagacataa gcatgatgac aaccaacagc ataacttcca atcatcaggc cttccaaaaa
    22621 aaggtgagga tctttaagca tcagttcgcg atccttacaa gtccccggtt cagattcatc
    22681 agcattggca accagataac tgggtttacc ggttcctttt gccatgaagc tccatttcaa
    22741 acctgtcggg aatccagctc cgccccgtcc gcgtactccg gattctttga cttcctgcac
    22801 aacttcttcc ggcttcatgc caaatacttt tttcaaagat ttaaacccac cagttgtttt
    22861 gtaaccggcc agagacttat cgtatttggg gtttctaata tttttgaaaa gaacaagggt
    22921 ttcttctagc atttttactg tttgagttga tcaatcaatt tatccatttt atccggagtt
    22981 acctgttcat gatattcttc atttacgatt attgcaggtg caaagctgca cgcaccaagg
    23041 cactccattt cttcaatgct gaattcaccg tcagttgtag tatgtcccgc gtgaatgttt
    23101 agtttttttt ctgtgtgttc caacaactct gttcctccaa cgaggcaaca tggtacgcaa
    23161 gtacaaacct gcaaatggaa tttcccggga cgtttcaaac gaaacatagt gtaaaaggta
    23221 accacgcctt caacatgagt aagcggcact tcgcaaatgt ctgccacgtg ctgcatccac
    23281 tcttccgtca accaaccatc attttcattc tgagccagaa gtaaaattgg aatcagtgca
    23341 ttttgcttga tgggataacg actcagaaga ttgtcaactt tagtctgaat gtccttggag
    23401 aacaaattaa aacctctttt ttcgatgcat tgtagaaata atacgagtct tagttctatc
    23461 aaatactacc aaaaaatcaa taaaattcgt ttatcaaagg tgttttttat attaaatgtt
    23521 ttgtgcttcc tgtgtgtatc attaattttt tattattaaa accaactatt atttgtatta
    23581 tattcttaat gttgactttt tatttatagt cgaatgccat ttaatcactt tttattgtca
    23641 taattgacaa ctgtttttat tcaaaggtat tattatttaa tattttttca caatgtttgc
    23701 gtttttactt gaggcggcac taaaatagtg gtatatttta ggttaaacta tacatttgtc
    23761 taaactaaga acatgaaaaa acgcttgaac ggttctcaaa ttttggttga agtcctgaag
    23821 aaacaaggag ttgagtatat tttcggatat cccggtggtg cttgtatgcc tatttttgat
    23881 gcacttgtag atgctccgga aataaaaata attttagtac gtcacgaaca aggggcaaca
    23941 cacatggcgg acggttacgc gaaagctact ggaaaaaccg gagtggtttt ggtgacttcc
    24001 ggacccgggg cgactaacac cgtcactggt atactcactg ctcatatgga ttcagtacct
    24061 cttgtggttc taacaggaca aaccatcacg cccaatttgg gtaaagatgc ttttcaggag
    24121 gccgatgtgt ttggtgtaac aatgcctgta gtcaaacaca gttatctggt cagggatgta
    24181 aaagacttgt caagaattgt taaagaagca ttccatcttg cctcaacagg tcgtcccggg
    24241 cccgttttgg tagacttacc caaagatgtg gtttctgaag aatgggcaga agatttttcc
    24301 gcaaagatgg atctgccggg ttattcagta cctaaaagtg ggaactccaa cagcattaaa
    24361 aaaatctctg agttgctggc aaaatcaaaa cgccccctgc tttatgttgg agccggtgca
    24421 gtaatttcct cagcaggaaa acaagtaaaa aaactggcag aaaaacttcg ggctcctgtt
    24481 accacaactc tgcttggaaa agggagtatt tctgaaatgc accagctttc agttggaatg
    24541 ctgggtatgc acggaacagc ttatgccaac aaggcagtta tcgagtgtga tctgattatg
    24601 gcaatcggcg cacgctggga tgatcgcatt acgggcaaag ttagtgaatt ttgtccaaac
    24661 gccacaaaaa tacatataga catcgatcct gctgaatttg gaaaaatcat tcaaccggac
    24721 atcagcgtaa atggagatgc agctttggtc ctggatgaat tgattcctct ggtaaagccg
    24781 cttgacacca aagagtggct gaagaaaatt gagaactgga aaaagaaaca ccccttaaaa
    24841 tatccaaagc gtggaggttt gcgcgcacaa catgttttag ctgaactgga ccgtttaacc
    24901 ggatccaatg ctattattgc tactgatgtc ggccaacacc agatgtgggc ggcccaattc
    24961 tgcagatcaa cccgcgaacg ccaatggctg tccagcggag gagctggaac catgggatac
    25021 ggattccccg cagctattgg agcgcaactt ggaaacccag aagatcttgt agttgctgtt
    25081 gtgggtgacg gcggtttcca aatgactatg tcggagctgg caacggctgc aattaacaag
    25141 ttgccgctca aaattttgat tatcaacaac agctatctgg gcatggttcg gcaatggcaa
    25201 cagctgtttt ttgataaccg tgaaagcgga gtagacatgg aaggtaatcc ggattttgtc
    25261 aaaattggtg aagcatacgg aatcaagggt tttcatctga ggcgtgcagg tgatgtgaca
    25321 aaagtgttaa aaaaagctct agcctataac gatggtccct gtattattga agcagaagtg
    25381 gtcaaagctg ataatgtttt tccgatgatt cctgcaggag ccgctgtcag cgatatgtta
    25441 attgatgaac ctccggcaag caagaaactt aaaaagcccg ttggcagtac ttgatctctg
    25501 aaataggtga aaaatcagct ttcattaccg tttaattatt atacttttaa tcgatgacct
    25561 gctgaatagt cgatcataat taatcacaat caggctttta tggaagaaaa atttgcaatc
    25621 agtatttatg tttgtaacaa gcccggagtt cttgtcaggc ttgctcaaac atttgcgcgt
    25681 cgtggttaca acgtggactc actggtcgtt tcaccggcac acaacaccaa cttttcaaga
    25741 attacagtag tagtacaagg tgtaccaagc tcatacgacc aaatactgcg gcaactgaat
    25801 aaattagttg atgttgttca cgccgccagt cacccttcta cggatattgt ggatcgggag
    25861 atggcattat ttaaagtaag ggcaaagcct gatcagcgtg cagaaatttt ccagattgta
    25921 gaggtgtttc gtgcaaaaac agtagacatt acggatcagt ctcttgtgat tgaaacgacc
    25981 ggggattcct taaaaatcga tgcccttgaa aaactgctgg agccaatggg gcttgtggaa
    26041 atggtgcgtt ccgggaagtt gataatgaag agggggcttg aggaaacctg agaaccactc
    26101 tgccctttcc cgtacatata tttggttaag ttctatctgt tcaattttac tggaagaacc
    26161 tctacagttt cttccagtaa aaattctttt tgaatgatac tttgaatcga agcagctaat
    26221 ttttgaaaac acacttttct attggaagtt ttccgaaata ataatccaac tcttctggca
    26281 ggcgcagggt ttttaaacgg aatatatttg atagaagcat tttgaattag attattataa
    26341 tcaattgcag tttgtggaat cagggtaatt ccaataccag tagataccat atgtctgatt
    26401 gtttccagac ttgtaccctg gaaatctttt ctttctttgg tcatggcttg tgaacaaaca
    26461 tccataacct gccctctcaa gcaatgacct tcttccagta ataatagagt ttcatcctgc
    26521 aaatcctcta tcttgataga taatttagca gcaaatgggt gctgattgtt agcagcaaca
    26581 taaaatggtt ctgtgtatag aatgatctct tcaagtcctc tcatttctag aggaactgct
    26641 aagattcctg catccaatgt tccttcactc agttttttca acataacttc tgtttgaact
    26701 tcctgtaaga taatttccag ttgaggatag gcttgattta taggttgcac aatcaagggc
    26761 aaaagataag gtgctattgt tggaattaga ccaacattga gtgtaccgct cattggatct
    26821 gtataggttg aaaccatatt ttcaatggaa gccgcatcag ccaaaattct ccgtgatatt
    26881 tttactattt cttcaccaat gggtgtcaaa aagacatttc tggtggttcg ttctaccagt
    26941 tgagcgccaa tgtggtcttc taattttttt agttgtccgc tcaaagtagg ttggctcaca
    27001 aaacattttt ctgcagcctt tcgaaaatgt tttaattcgc cgatggctga aagatattcc
    27061 agatctctca aattcattaa acactcctca aacaaaaggt taatctgtat taattgatgt
    27121 taccaatcag aataacaaaa ttaaaagatt aaacaaatca atttaaatat tttatattta
    27181 cactgagttc attgagggct aaaggggagt ttatacccgg ggtttagagc agacttgagc
    27241 atgagaaaga gagatatgga acttcagtca gcaggctgat ggcgtcgcgc cgtccacttc
    27301 aagtcgcatg gacgatcacc cgattccgca ggtctttacc gccctggtca gatcgtatcc
    27361 gctttcgtca atgttgacct gttcggtggc accatcattg aaggccatgt aggcgaagat
    27421 ttcaaacccg ttggtcttga tgacgccata gttttagagg tggctcggca ggtgcttgtt
    27481 ggagccggtc ggcgtgaacg caccaggcac tgaaaacagc gcaacttttc taccattaaa
    27541 cagttcggca gtactcttgt ctttgaccac gctgtctgtc atcatcttta aacttccttc
    27601 aggcatctga tctccattgg aaatggacat gttcgttgtc tccttcgtta tttcggttgg
    27661 gtgctcactc tgagatttct ctagtcggaa tgcaaagatt gcaagaagca ccttctcatt
    27721 tgaaagtttt taatgacttt gccaaggcgt attgttgttc cttcatttct tcgctgctct
    27781 ctatcgatca ttgaattgat agtcaatgcc tgtcaaagtc atcgaaacaa acgattagac
    27841 aaaaccgatt ctcttctcta ggataaccat attatctaat cacagtttta ccgttcatcc
    27901 cctaacgctc ctgagggagc ctcacatatt aagaaggaga attatgacta ctgaaagcaa
    27961 atgtcctgtt atgggcaaaa cacatcaggc cgctggcggc acagcgaacc aacactggtg
    28021 gccgaaccag ttgaacatgc ggatcctgcg acagaaccat ccacaggacg atcccaacgg
    28081 tgagggattc gactacgcaa aggagttcaa gagcctcgac tttgaggccc tgacgaagga
    28141 cgttgatgcc ctgatgaccc agtcgcagga ctggtggcca gccgactatg gccactatgg
    28201 accacttttc atccggttga cgtggcacgc ggctggaacg tatcgtatca cagacggtcg
    28261 cggcggtggt ggtgcgggtg cacaacgctt cgccccactg aacagctggc ccgacaacgt
    28321 caaccttgac aaggcacgga ggctactctg gccgatcaaa caaaagtatg gacagaagat
    28381 ctcctgggcc gacctgctta tcttcgttgg caaccgcgct ttggagacga tgggcttcaa
    28441 gaccttcggt ttcggcggcg gccgcgagga catctgggcc cccgacgagg acacgtactg
    28501 gggaccggag accgtctggc tagacgacga gcgctacagc ggtgataggg agctggccga
    28561 acctctcggc gccgtacaga tgggactaat atatgttaac ccacagggtc caaacggcaa
    28621 tccggacccg ctgttggccg cgcgagacat ccgtgagacg ttccgccgga tggcgatgaa
    28681 cgacgaggaa accgtggctc taattgctgg tggccacacg ttcggcaaaa cacatggtgc
    28741 tgccgaggaa cattacaaag gtcccgagcc agagggcgcg aagattgctg agcagggctt
    28801 cggatggacg agcagctttg gcagcggcaa aggcggcgat cagatcggca gcgggctgga
    28861 gggcgcttgg accaccgagc cggcgaagtg ggacaacggc tactatgaga atctgttcga
    28921 ctacgagtgg gagctgacga agagcccggc cggtgcgcat cagtggaccc ccaagaactc
    28981 cgaagcgcag ggcaccgtgc ctgacgccca cgattcgtcg aagacgcacg cccccacgat
    29041 gctcacgacg gacctcgcgc tggtcaccga tccgatttac aaagagatct cgaagcggtt
    29101 ctacgagaac atggacgaat tcgccgacgc atttgctcgc gcttggtaca agctaataca
    29161 ccgagatatg ggacccgccg tgcggtacct cggaccgtgg gttcnnnnnn nnnnnnnnnn
    29221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnnnn nnnnaagcta atacaccgag atatgggacc cgccgtgcgg
    29341 tacctcggac cgtgggttcc caacgaagaa ctactttggc aggatcccgt tcctgccgtt
    29401 gaccacactc tgatcaacga tgcggacata gggtctctca aggccaagat cctcggctca
    29461 gatttgtcga tctctcagct ggtctcgacc gcttgggcgt cggcctcaag ctatcgtgat
    29521 acagataagc gaggtggcgc gaacggcgca cggattcgcc tttcaccaca ggctgaatgg
    29581 gacgttaacg tggcctctgg catagccccg gtggtggcaa ctctcgaagg gatccagcaa
    29641 gagttcaaca actcgcaaac aggggggaag aaagtttcgc ttgccgacct aatcgtgctg
    29701 ggtggttgcg cagccgtcca agaggcggcg aagcgtgcgg gccaggacgt ggaggtcccc
    29761 ttcacgcctg ggcgcaccga cgcatcacag gagcaaaccg acgtcgagtc gttcgcattc
    29821 ctcgagccgg tcgcagacgg gttccggaac tacctccggg cgggccataa cctccagaca
    29881 gagcacctgc tggtcgacaa ggcgttcacg ttgaatctga cggctcccga gatgacggtc
    29941 cttgtcggcg gcatgcgtgt cttgaacgcc aacgccggag cgtcgaagca cggggttttg
    30001 actgaccgtc cagagacgct gaccaacgac ttcttcgtga atgtgctcga tatttcgacg
    30061 gagtggaagg ctacgtcaga cgccgagcaa gagttcgagg gtcgtgatcg taagagtgga
    30121 gaactccgat cgaccggcac tagggtcgac ctagttttcg gttcgaactc tgaactccgg
    30181 tccctcgctg aggtctacgc tagtgacgac gcgcagcaga agtttgtcag tgactttgtg
    30241 gctgcgtggg acaaggttat gaatctagat cgcttcgacc tatgatccat gtctgcggaa
    30301 tggttcatag agagaagtat cacaaaaaat gtgaaacaag atatttccgg atatcttttg
    30361 cttctttcat tggaccatct tgagttatta ggcaggggac catcacatcc cctgtcagtt
    30421 ctacaagttc tttcgcatag tccggattca ggcggatatc cttgaatgta aatttatctt
    30481 ttatttttag cgtggaaatc gtattcagca caatctgcga atattcacag tcttcataaa
    30541 aataaagttc cattcactgt gtgtcagatt aaaattccca aaaaaaaagg cggtcttaat
    30601 tgagaccgcc tgtatgattt taaaaaagat ttccagtcgc tcagctgtta acctttttgc
    30661 actcagctaa aagctcgcca ataatgcgct ctccatctgc cataacggaa tatcgaaccg
    30721 agccttgctt gtctactata actgttgatc gagctgcgaa tcccttattc tccaaataag
    30781 ccccataaga agtcgacatg gcgccttttg gatggaaatc ctgcagcagt ggataagaca
    30841 caccgccaag ggattcggcc cattttttat gtgaatgttt actgtcaatg ctcacaccca
    30901 atacttgagt gtctgcctcc ttgaatcgat caatcatcga ctcaattttg ggtatttccc
    30961 tgcttcaggt ggcagaccaa tcaagtggat aaaacaccag cattacattc ttttctccct
    31021 tgaactcact caaggaatac tcactatcaa actgactgac caatttaaaa tccggtgctt
    31081 ccgaacccac tgcaatcatt aatacctcca aagaattatt tgaaaaagcc ctgaaaacaa
    31141 catgaattca gagtaaacgt aaactgtgac taacagccga aagatgggga taactcggtt
    31201 gtcaaattga tctgactaaa cttatttact cagttgattc aatgattgtc cgatcggcaa
    31261 aaacggaaga acattccttc ccaactcttt gtcagcaatc accttgttta ctttggggag
    31321 aatgctggcc attgtttcta gcagaatccg ctcccgggta atatctttgg ccattttata
    31381 ttcttcatag atcagattaa atctttctga ttcaccaatg gcgaccaaca ccttttcttt
    31441 ggcataaccc tttgcactat tgaccagctg tgcagcttct cctctggctt tcgggacaag
    31501 attgtttctg taaccttctg catcattaat cattttttca cgatcctcac gggcactgac
    31561 cacatcttta aaagcttctt ttacagcatc cggaggatga acatcctgca gtttcacatt
    31621 ctcaatctta attccgcctt tgtaactgtt cattgtttgc tgaagaagca gagcagtagt
    31681 atcttcaatt attccttttc caacagtcag tgcttccgta actgtagaat caccgataac
    31741 ttcacgcatc gcggattcag cggcagatct cacagttaca tccggattag tcagactata
    31801 aagataattt tccaaatcag aaatgcgata ctgcacaatg aactgcacat caatgatgtt
    31861 ttcatctccg gtgagcatta atgattcttt taaaacacgt ctgtatttgg caggaggtcc
    31921 agtctgtatt gtacggaatc caatctcaag tctccgcact gcagttaccg gagcaatctg
    31981 gacggtttcg atcggagacg gtaaatgata atgcggtccc ggtccaacca cttctgtaac
    32041 ctctccaaaa cgcttgatga ctccctgttc ttcaggatca ataataaaaa tacctgttgc
    32101 caaccaaacg ataattgcta aaattatgat gtaggaaaat accccttttt ttggcagacc
    32161 aatcttaaac ttatcctgaa gattgctgag gagttcatca atgtcgggag gttgcggccc
    32221 tttttcccaa ggtgatttat ttggcggttt gttccaggta cccattttaa attttcatgt
    32281 ttaagattgt aatagattag acaataaatt aagcgaagac tgcggaaaat acaagcttaa
    32341 tgctcacatg catacaaaat aattgaaaac agtattttga tgaactagt
//
LOCUS       DTVD01000074            2603 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16478_length_2603_cov_268.160911, whole genome
            shotgun sequence.
ACCESSION   DTVD01000074 DTVD01000000
VERSION     DTVD01000074.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16478_length_2603_cov_268.160911"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            16..447
                     /locus_tag="EYO68_02795"
     CDS             16..447
                     /locus_tag="EYO68_02795"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIC06764.1"
                     /translation="MVHLAKRSELVSKPGFPSPKNGICRYTLLFKRGSNVMRNSSKSI
                     LNDLEKCLRKFPQQIIQITGHSDPLKPDFGEGSKFSNVTLGLKRAEKTMATLVKLDFN
                     KGRFVVISKGGKEPVAKGKKEQNAGKDRRVEVLSKPNFESF"
     gene            609..>2603
                     /locus_tag="EYO68_02800"
     CDS             609..>2603
                     /locus_tag="EYO68_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIC06765.1"
                     /translation="MGLADFGTIALMAAFALCVYGTVVPHLGVRTNNWNLVRSAQNAS
                     ILTFFLVALASAILIHALITSDFSIIYVWEHSSTDMPLSYKFSSFWGGMNGSLLFWIL
                     VQSFFAMIVAYRYQYSNREIIPYVIATFNAIMAFLLLLLIGWSNPLELQTVIPEEGRG
                     LNPLLQNPAMAIHPPSLYLGFIGFSVPFAFAMGGLIRGKMDNEWVLTTRRWTLMAWYF
                     LSSGLILGGQWAYEELGWGGFWAWDPVENAALMPWLTGTAFLHSVMIQEKRNMLKIWN
                     VVLIIMTYGLTILGTFLTRSGVINSVHSFTQSEVGPIFLIYLALIMIIGFALLFKRIQ
                     MLESEYKIESVLCRENVFLAQNVLLVGIAFTVLLGTTFPLLSEAVRGAKLSIQAPFFN
                     TITAPMGYALLLLMGVGMLISWRKSSWEGLKGDFQNPVILATAGTIFLSWLLYEKNLQ
                     WGVYITFWAAIFVTSIFLFELAKTSYIKSRQTETNFLMGLFYAFSSNRRRYGGFLIHF
                     GAVLMFLGFAGTFFSLERDITLEPGSSRQIGDYRLEFQNVSEFQLGNARHRAAIIEVF
                     DNEGNFLEVMKPAKSFYPTQPQPLTEVAILRSFMEDLYLIFSSENGDEKGSVTLRVYI
                     NPLIGWAWMALPVFTLGVGISLTYRPKSLLTRETILKERYI"
BASE COUNT          707 a          486 c          568 g          842 t
ORIGIN      
        1 tcaagaagtc attttgtggt ccatttggcc aaacgttcgg agttagtttc aaaacctggt
       61 tttccttctc caaaaaacgg tatttgcagg tacacacttc ttttcaaacg tggaagtaat
      121 gttatgcgaa attcttcaaa atcgattttg aacgatctgg aaaaatgctt acgtaaattt
      181 ccccaacaga taatccaaat tactggtcat tccgaccctc taaaaccgga tttcggagag
      241 ggttcaaaat tcagcaatgt aacattaggt ttgaagcgtg cagaaaagac catggcaacc
      301 ctggttaagt tagacttcaa taaggggcgt tttgttgtca tcagcaaagg tggaaaggag
      361 cctgttgcca aaggaaagaa agagcagaat gcaggaaaag accgcagggt tgaggttctc
      421 tcaaaaccaa actttgaatc tttttaatca tctcaacagt cacgagtttt atcatgtcat
      481 cccaaacgta tacaagattt ctcgcttcac tcaaaatgat acgatttcag gacttcttat
      541 aactgtatca tactatggta ggagagaata ttttgtgaaa aacggtcatt aattatttag
      601 gaaaatttat gggattagct gactttggca ccatcgcatt gatggcggca tttgcgcttt
      661 gtgtttatgg aactgtggtt ccgcatttgg gtgtacgaac taataattgg aatttggtgc
      721 gctcagcgca aaatgccagt attctcacat tttttttagt agcgctagcc tctgcaatac
      781 taatccacgc cttgattacc agtgatttca gtataattta tgtctgggag cattccagta
      841 ctgacatgcc tttgtcctac aaattttcat cattttgggg tggtatgaat ggctcattac
      901 ttttctggat tttagtacaa agtttttttg ccatgatagt tgcgtaccgt tatcagtact
      961 caaatcgtga gatcattcct tatgtgattg ccacattcaa cgcaataatg gcttttttgc
     1021 tgctgttgct cattggctgg tccaaccctc tggagttgca aacagttatt ccagaagaag
     1081 gtcgcggctt aaatccactc ctgcaaaatc cagccatggc tattcacccg ccgtctttgt
     1141 acttagggtt cattggtttt agcgttcctt ttgcctttgc aatgggggga ctgatccgtg
     1201 gaaaaatgga caacgaatgg gtactgacaa cacgtcgctg gacattgatg gcctggtatt
     1261 ttttgtcgtc aggactaatt ttaggcggac aatgggcata cgaagaactt ggctggggag
     1321 gtttttgggc ctgggatcca gtagaaaatg cagcgctgat gccctggctg accggaacag
     1381 cctttttgca ttcagtcatg attcaggaaa aacgcaatat gcttaaaata tggaatgttg
     1441 tgctcataat tatgacttat ggtttgacca ttttgggtac gtttcttact cgatcaggtg
     1501 taatcaattc agttcattca tttacacaat ctgaagtagg ccctatattc ttaatatatt
     1561 tagctctgat tatgattata ggttttgctc tgttattcaa acgtattcaa atgttggaat
     1621 ctgaatacaa gattgaatcg gtgctttgta gagagaatgt ttttttggca caaaatgttt
     1681 tgctggtagg aatagcattt actgttttac ttggtacaac atttccttta ctttcagaag
     1741 ctgtacgtgg agcaaagctt tcaattcaag caccattctt taatacaatt acggcaccaa
     1801 tgggttatgc tttattattg ctgatgggag taggaatgct gatttcctgg cgaaaatcta
     1861 gttgggaagg gttgaaggga gactttcaaa atccggtaat tttagctacg gccggcacaa
     1921 tatttttgtc gtggctgttg tatgaaaaaa atttacagtg gggggtttat atcacttttt
     1981 gggctgcgat atttgtaact tcaatttttt tgtttgaact tgccaaaaca tcgtatatta
     2041 aaagtcgaca aactgaaacc aatttcttga tgggtctatt ttatgccttt tccagcaatc
     2101 ggcgtcgtta cggaggattc ctgattcatt ttggtgcagt tctgatgttt ttgggctttg
     2161 ccggaacctt ttttagcctg gagcgtgata tcacacttga accgggaagt tccaggcaaa
     2221 taggtgatta ccgtttagag ttccagaatg taagtgaatt tcagcttggc aacgctagac
     2281 atcgtgcggc tattatcgag gtatttgaca atgaaggaaa ttttctggaa gtaatgaaac
     2341 cagcaaaaag tttctatcca actcaacctc aaccacttac tgaagttgcc attttacgct
     2401 cattcatgga agacctctat ctcatctttt cgagcgaaaa cggtgatgaa aagggatctg
     2461 ttacacttcg tgtttacatc aatcccctga taggttgggc ctggatggct ttacctgttt
     2521 tcacattagg cgtgggaatc agcctgacgt atagaccaaa aagcctcttg acgcgagaga
     2581 ccattttgaa ggaacgttat att
//
LOCUS       DTVD01000075            2583 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16704_length_2583_cov_332.980222, whole genome
            shotgun sequence.
ACCESSION   DTVD01000075 DTVD01000000
VERSION     DTVD01000075.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_2583_cov_332.980222"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(130..1164)
                     /locus_tag="EYO68_02805"
     CDS             complement(130..1164)
                     /locus_tag="EYO68_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIC06766.1"
                     /translation="MSNKINRQIILAKRPSGKPQPADFSLVDASVPVPEEGQVLCKTI
                     FLSLDPYMRGRMNDGKSYAAPLEIKEVMTGGTVGQVLESRSSNFHEGDIVFGSGGWQD
                     YWASEGKALRKIDPQLAPVSTATGVLGMPGVTAYTGLLNIGQPKKGETVVVAAASGAV
                     GSVVGQVAKIKGTRAVGIAGSAEKCRFVTEELGFDACVNHKSPDFTEALKNACPDGID
                     VYFENVGGKVFQKVLPLLNDFARIPVCGMISHYNATELPPGPNLTPLLMRDILVKRLT
                     FRGFIVWDFASQEKEALTQLATWIQQGKLHYREDIVEGLENAPEAFIGLLEGRNFGKL
                     VVQVSDHPAA"
     gene            complement(1295..2443)
                     /locus_tag="EYO68_02810"
     CDS             complement(1295..2443)
                     /locus_tag="EYO68_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIC06767.1"
                     /translation="MVAQMKSLREQLANCQLPAEQAFSIPPECYTSEELLQEELEKVF
                     QHNWIGLGRADRFAVSGDYETMNLGGKPVIVLRDHDGILRAFANTCRHRGARLLNDDG
                     NCKHIRCPFHAWTYKLDGSLSGAPHMNKVSGFERSDYGLISYHAEERLGFAFVCLDPS
                     APDLDDYLGDFAEIHAPWPIESLVTTRRRSLTVDCNWKAFLDVFNEYYHLPFVHPDSL
                     DEIYNFPTPADKVTGAFASQFGNTEGTGGLLQDSEEQPLPSMTNLQGRAASGVQYTWV
                     FPNMTFAAGMDVLWIYEAYPLGADCCQVYQSICVPPETAALPDLEEKIAAYYQRFDAG
                     IEEDVPALVNQQRGLASSDARQGRFQPHLEANVASFARWYADQWLRQS"
BASE COUNT          690 a          621 c          580 g          692 t
ORIGIN      
        1 cttttggatg tagctactgt cttgaattgg atgcaactcg agattgacaa tggtttccgg
       61 attcataatt ttccaaaggt ggttgaagca ttttagtgat taataattgt ttctgtataa
      121 atttatgatt caagcggcag gatgatcact aacctgaacg accaatttac caaagttcct
      181 accttccaga agtccgatga aggcttccgg agcgttttca agaccctcca caatatcttc
      241 acgataatgt agttttcctt gctgaatcca agttgcgagt tgtgttaaag cctctttttc
      301 ctgactggca aaatcccaga cgatgaagcc gcgaaaagtc agacgtttga ccagaatatc
      361 ccgcatcagc aacggtgtta aatttggccc cggcggcagt tctgtggcat tgtaatgcga
      421 gatcattccg caaacaggaa tgcgcgcaaa gtcattcagc aagggcaaaa ctttttgaaa
      481 tactttgccg ccgacatttt caaaataaac atcaattcca tccggacaag catttttaag
      541 tgcctctgta aagtccggac ttttgtgatt gacacaagca tcaaagccga gttcctcggt
      601 gacaaatctg cacttttccg cagaaccggc aatgccgact gcgcgcgtac ctttaatttt
      661 tgcgacctgc ccaaccaccg aaccgactgc tccggatgcg gcggcgacta caaccgtttc
      721 accttttttt ggttgtccaa tgttgagtaa accggtgtaa gccgtaaccc ctggcattcc
      781 caaaacaccg gttgcagtgg aaactggtgc aagctgagga tcaatttttc tcaacgcttt
      841 accctccgat gcccaataat cctgccagcc ccctgaaccg aagacaatgt cgccttcgtg
      901 aaaattggag cttcgggatt caagcacctg cccgactgtg cctccggtca tcacctcttt
      961 tatttccaat ggtgcggcat aagatttgcc atcattcatg cgtccccgca tatacggatc
     1021 aagagacaaa aaaatggttt tacataaaac ctgtccctct tccggaactg gaacagaagc
     1081 atcaactaaa ctaaaatcag caggctgcgg ttttccgctt ggacgctttg ctaagataat
     1141 ttgacggttt attttgtttg acatattgac ctgttttaag gatttaaaaa gatttaatgt
     1201 ttgcgaatag ttttacagaa atgcacaaaa tactctgaga cacttcccgg tctgatgttt
     1261 taactctaaa gaatacacag tatatctcct catctcaaga ttgtcttagc cactgatccg
     1321 cataccacct agcaaaggaa gcaacattcg cttccaggtg cggctgaaat cgcccctgac
     1381 gagcatcgga agaagcgagc ccacgctgtt ggtttaccaa tgccggaaca tcttcttcga
     1441 tcccggcatc aaaacgttga taataagcag ctattttttc ctctaagtcc ggaagtgccg
     1501 cggtttccgg cggaacacaa atgctctggt aaacttgaca gcaatccgcg ccgagtggat
     1561 aggcttcata aatccagagt acgtccatac cggccgcaaa ggtcatgttt ggaaataccc
     1621 aggtgtattg gacaccggaa gcggcacgac cttggagatt cgtcatgctt ggcaggggtt
     1681 gttcttcgga atcctgtaac agtccgccgg tgccttctgt gtttccaaac tgactggcaa
     1741 aggctccggt cactttgtcg gctggagttg gaaaattgta aatttcatca agtgaatccg
     1801 gatgcacaaa gggcaaatgg tagtattcat tgaatacatc cagaaaggct ttccagttgc
     1861 agtcaacagt caacgatctt cgccgtgttg tcaccagtga ttcaatgggc cacggagcat
     1921 gaatttctgc aaaatcacca aggtaatcat caagatcggg cgcagaagga tcgagacaga
     1981 cgaaggcaaa accaagccgc tcctcggcat gataactgat cagtccataa tctgacctct
     2041 caaaaccgga aaccttattc atgtgcggcg ctccggagag agagccatcg agtttgtaag
     2101 tccaagcgtg aaacggacag cgtatatgtt tacagttgcc gtcatcgttc aaaaggcggg
     2161 cgcctcggtg gcggcaagtg ttggcaaaag ccctcaaaat tccatcatga tcccgtagta
     2221 caatgacggg ttttccgcca agattcatcg tttcataatc cccggagact gcaaaacgat
     2281 ctgcacgccc taacccaatc cagttgtgtt gaaaaacttt ttcgagttcc tcctgcaaaa
     2341 gttcttccga agtgtagcat tccggtggaa tcgaaaaggc ttgctctgcc ggcagttggc
     2401 agttggcaag ttgttcacgc agggatttca tttgagcaac cattgagtga accctccaat
     2461 ggggttgttg gttttcaact cttgaacagt tgagttcatg gggggattaa ttatgaaatg
     2521 gagtgtggat tttgttcact tggtatcata aaccttatgt ttaagtcgtt ccacttcagc
     2581 ttg
//
LOCUS       DTVD01000076            2566 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16881_length_2566_cov_140.127041, whole genome
            shotgun sequence.
ACCESSION   DTVD01000076 DTVD01000000
VERSION     DTVD01000076.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_2566_cov_140.127041"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            222..1235
                     /locus_tag="EYO68_02815"
     CDS             222..1235
                     /locus_tag="EYO68_02815"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIC06768.1"
                     /translation="MSKLRSFIEELPKCELHVHIEGTLEPKMKLQLAKRNKVELPYKN
                     EKAVRDAYEFDDLSSFLTVYYEGMSVLLTEQDFFDLTYAYLKKAHSQNVLYTEMFFDP
                     QAHTARGVSFDTVIRGIRRAQMQAEISFGIRSQLIMCFLRDWSAEFAMSTLLQSIPYK
                     EWIIGVGLDSDERNNPPIKFVEVFKRAREEGYFLTMHCDVDQKDSVNNIWQTVKDIGV
                     DRIDHGVNSLEDMKLCEEIKEQSLTLTVCPISNSFVSDGSKSEAILSMLENGMRVTVN
                     SDDPAYFPGYMTENLICVQKDVQLGKRELVQLAENAFEGTWLPRSTKDRFIEDLKNYV
                     TKY"
     gene            complement(1479..2420)
                     /locus_tag="EYO68_02820"
     CDS             complement(1479..2420)
                     /locus_tag="EYO68_02820"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:PRK05627.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIC06769.1"
                     /translation="MQVFKFQKNTANIPSSVVTIGNFDGIHLGHRALISAAVQEAKTR
                     GCTSALVTFDPHPQEIIHSQQSVPRICTSSHQLRLLEEIGLDEVHVIPFTKELAQLGP
                     EEFAFQFLIKRFDLKKLVIGYDFRFGKHRAGDFKLLEILSQKYNFSLEEVAPVQERSQ
                     TVSSTLIRQLIRKYRFAEIPHYLGRDYSVYGQVEQGEQRGKNLGFPTANILPGVTLAL
                     PEGVYVSKIKLAGKTHYGVTNVGKRPTFGENSVTVETFIFDFEKVIYGEYLEVIPLSQ
                     LRPEMKFSSVEELKTQIRQDIETARNYLDTNEPQPVI"
BASE COUNT          813 a          480 c          505 g          768 t
ORIGIN      
        1 acggcggatc cgattcgaca ataagcttat cgtccatgaa caccaaccgg tgcgctttag
       61 aatgaagaat actgtattat aacacacctg aataaagata tatactattg atattattaa
      121 tttataacaa ttattaagtg tcaactaagt aagacaatta tcattaaaac ttataagttg
      181 aagttgttta caactagatt aaaattttag gatggagtga aatgagcaaa ctaaggtcat
      241 tcatagaaga gctgccaaaa tgcgagcttc acgttcacat agaaggcaca ttggagccaa
      301 agatgaaact ccagctggca aagcggaata aagttgaact accctacaaa aatgaaaaag
      361 cagtcaggga tgcttatgaa tttgatgact tgtcttcctt cctcacagtt tattatgaag
      421 gcatgagtgt tttgcttaca gaacaggatt tctttgatct aacctatgca tacttaaaaa
      481 aagcacattc tcagaacgta ctttacacag aaatgttttt cgatccgcag gctcacactg
      541 cccgcggagt cagttttgat acggtaatcc ggggaattcg ccgtgcccag atgcaggcgg
      601 aaatttcgtt tggaatccgt tcgcagctta taatgtgctt cctcagggac tggagcgcgg
      661 agtttgccat gagtacttta ctgcagtcaa taccctataa ggaatggatt ataggggttg
      721 ggcttgattc agatgagagg aataaccccc caatcaaatt tgtagaggtt ttcaaacggg
      781 cccgtgaaga aggttatttt cttactatgc actgcgatgt tgatcaaaag gactctgtaa
      841 acaatatttg gcagaccgtc aaggacattg gcgttgaccg aattgatcat ggagtaaatt
      901 cacttgaaga catgaaactt tgtgaggaga taaaggagca atcacttacc cttaccgtct
      961 gcccaatctc aaattcattt gtttctgatg gtagcaagtc tgaagcaatc ctgtctatgc
     1021 tggaaaacgg aatgagggtt accgtcaatt cggatgatcc ggcctacttc ccaggctata
     1081 tgactgaaaa cctgatttgt gttcaaaagg atgttcaact tggcaagaga gaacttgtgc
     1141 agcttgccga gaatgctttt gaaggcacat ggctgcctcg ctccacgaaa gaccgcttca
     1201 ttgaagattt aaaaaattat gttactaaat attaatgaaa ctgtaaaaag tcctaaaacc
     1261 gtgtcatttc gagcgaagcg aaaaatattc tataagctac tatgctaata tcattgtatt
     1321 ttttcccttt gactgaaatg actgtgtgga tttcagactt ttttagaaac catcaaaatt
     1381 aataattgga attaaacaga gagcaattaa aaagttacag gagttatgag gtaaatgcac
     1441 taaagtgact tttcattaaa gcatttttct caaggtgatc aaatgacagg ttgcggttca
     1501 ttcgtatcca ggtaattacg tgcagtttca atatcctgcc tgatttgtgt tttaagttct
     1561 tctacagatg aaaacttcat ttccggacgc aattgtgaaa gaggtattac ttccaaatat
     1621 tcgccataaa taactttttc aaaatcaaaa ataaaagttt ctacagttac cgaattttca
     1681 ccgaatgtag gtcttttacc aacattggta acaccataat gagtttttcc agccaatttt
     1741 attttgctca catagacgcc ttctggcaga gctaaagtaa cacctggcag aatattagct
     1801 gtaggaaacc caagattctt cccccgttgc tcaccctgtt ctacttgacc atacacactg
     1861 taatctcgtc ccaggtaatg tggaatttca gcaaaacgat acttccgaat caattgccga
     1921 atgagggtgc tgctgacagt ttgacttctt tcttgtacag gagctacctc ttcaagtgaa
     1981 aaattatatt tttgactgag gatttccagt aatttgaagt ctccagcacg gtgttttcca
     2041 aaacggaaat cgtatcctat gacaagtttt ttcaaatcaa agcgctttat caaaaactga
     2101 aatgcaaatt cttccggacc cagttgtgct aactcctttg taaaaggaat tacatgtact
     2161 tcatcaagac ctatttcttc gagtaaacgg agttgatgcg atgatgtaca aattctgggt
     2221 acagactgct gagaatgaat tatttcctgc ggatgtgggt caaaagttac cagggcactg
     2281 gtgcatccac gggtttttgc ttcctggact gcggcactga tcaaggcgcg gtgacccagg
     2341 tgtataccat caaaatttcc gattgttaca acagaacttg gaatatttgc tgtatttttc
     2401 tggaatttga agacttgcat aaataactta agatactgaa attataatat atttttatat
     2461 tattgttttg agatgtcgtt tcagaaagca ccaaagaaaa ataaaaataa agttctaaaa
     2521 ccttaagtgt ttggtaaaaa tatacaagcc gtaattactc agaacg
//
LOCUS       DTVD01000077           10163 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1691_length_10163_cov_137.217847, whole genome
            shotgun sequence.
ACCESSION   DTVD01000077 DTVD01000000
VERSION     DTVD01000077.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10163
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_10163_cov_137.217847"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            155..877
                     /locus_tag="EYO68_02825"
     CDS             155..877
                     /locus_tag="EYO68_02825"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06770.1"
                     /translation="MLTLSKVTKRFGSQTVVDSLDLSIPQGLVFGLLGPNGSGKTTLL
                     RMAMGLLQPTSGRITIFENNSPGDSEVRKKLGYMPQQLAVYPGLSVRENVLFYGRLYG
                     IPENNLSTRSLEILEMVELESHQNKLAGDLSGGMLRRLMLATTLIHQPALVILDEPTA
                     GVDPSLRLRFWEWFEQLAQGGASILITTHHISEAGRCDKVAFLREGLLLEHDTPDTLM
                     RRYNSENLEQAFVQATQAKLED"
     gene            786..2048
                     /locus_tag="EYO68_02830"
     CDS             786..2048
                     /locus_tag="EYO68_02830"
                     /inference="COORDINATES: protein motif:HMM:PF12679.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06771.1"
                     /translation="MIRRIHSCDVTIRKIWNKLSFRLLKQSWRIEMVARWIAWRIIRE
                     ILRDRRTLAFFFLVPLIVMSLIYYALQEDETARLGVVSRGVMRLFEADLMLTLEEEDI
                     ELVSLDIPDEEIDPERLEQMIWTQLRTGQAEGILFLGEQLLEERFDGKRGRIDFYLEG
                     SRPTLTASVVESVASVMDDLASSLPVVIDSSCSAFCANSVNIKPLDLEEHYLYGSEDY
                     RLIDFFLPVFPPFFVFFFTFLISTITFQRERVRGTLERLLIAPVSFTQIVLGYVGGFI
                     IFSGCQAAIILTFILSLIGFKITLTQIGAIAFLTFLMLLISLVLGLLASFLAANEFQA
                     LQFIPLVILPQIFLSDMIWSIDGFPKIFQWISLALPLTHANTAMRNVLLKQQNIWDSW
                     PQLLTLSGFFFCILLLLVLVSRRHQNAA"
     gene            2459..2707
                     /locus_tag="EYO68_02835"
     CDS             2459..2707
                     /locus_tag="EYO68_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06772.1"
                     /translation="MIEGRIKQLDLANRSALIVEGNGNEVTVQFALRTNVEVIEPETV
                     GLMGGNLEDLEEGFEVEIEVASTEKDGSIICDSIACVS"
     gene            2871..3074
                     /locus_tag="EYO68_02840"
     CDS             2871..3074
                     /locus_tag="EYO68_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06773.1"
                     /translation="MDNEKSAGRRGFLLGVLTAMGATTATVLAARTYETNKDGVIDSD
                     ASTGPILYQRTEETERYYKTLYN"
     gene            3087..5876
                     /locus_tag="EYO68_02845"
     CDS             3087..5876
                     /locus_tag="EYO68_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIC06774.1"
                     /translation="MKLNRVIQNDGQDLNPAFMDSLNPSVSRRGFVTAAGMGVGLMAM
                     GPSLVREAKAATGAETARKLTQIKTICGNCAVGCGFIAEVENDTWVSIEPWFEHPINQ
                     GSLCSKGAAAREHVISDKRLKSPMKLEDGKWKRISWDTAMSEITTKLQEIRKKHGPDS
                     LMILGSAHHNNETSYALRKFAAFWGSNNIDHQARICHSTTVAGLANVWGYGAMTNSMN
                     DIRNSKSVLIIGENVCTSHPIAMQHILTAKEENRAQIIVVDPRFSQTAAFANKYVRHR
                     SGTDVAFIYGLVNIILKNGWEDKRMLQDRTYGFSNLKEELKRYDPETVSDITGVSVAD
                     LEHTARLIAENRPGTLIWAMGGTQHTNGTSVTRSYCVLQLVLGNMGKPGGGTNVFRGH
                     DNVQGATDLGVLSNTLPGYYGLSKGAYKHWSNVWGVDNNWINGRFANEKIQHKVGFTV
                     ARWYEGVLMDPKELGQDENVHAAFYWGHSTNSQSQLDRIKTALDKVELLVDIDPFVTT
                     TSILPDRKDGVYILPAATVYEQSGSVTNSNRDIQWRNQVVKPVWESRKDLDIVIDLAE
                     RLGFGDKFKKSKNGPWKQLPEDITREWNLGMRTIGMTGQTVERMKRQQDWAHAFDLKT
                     KIAEGGPVDGEQWGLPWPCWTDKHPGTHILYRTSIPVAEGGLGFRARWGVKAPDGNTL
                     LAGKGAAPVDSSINEGYAEMKNWKTDLTGKVFEEAIAKGLAPYGNGRARFNVWTFKDD
                     PVPKHREPLHSPRPDLITEWITYDDVKDHYRVPTLYKSLQKADWVEKYPLILSTGRQV
                     EFEGGGNSERNCWWLVELQPEMYVEIHPRLANDNRIRHGDWVWVESPEDTDGKPSRIK
                     VKAKVTRRVSPDMVYVPFHWGGVMSGVDLSNKYPEGYLPYGMGESANTITNYGYDRIT
                     QMQETKTGLCRIKKA"
     gene            5964..6539
                     /locus_tag="EYO68_02850"
     CDS             5964..6539
                     /locus_tag="EYO68_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIC06775.1"
                     /translation="MKFYVDVRRCIECGGCEVACKNENEVPPGIARIRVVTVNEGQAG
                     ETNVAVPCMHCSNAPCVSVCPVDALFNRADGIVHVNKETCIGCGYCLYACPFGAPQFP
                     KGSPFGKRGVMDKCTYCAGGPEEAFSTREQELYGSNRIAEGKLPICASICSTKALVAG
                     DAEEVANVVRSRAAARGSSSGAWGWSKAYNN"
     gene            6768..7766
                     /locus_tag="EYO68_02855"
     CDS             6768..7766
                     /locus_tag="EYO68_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HIC06776.1"
                     /translation="MEPIILMNSKWGNDIFTPMYLFFGGLTGGLFIVAVVADLVGIKY
                     KSFEKLSKVVAFMVPLALVLAGLFITFHLGKPERGMFFPLYFSNFNSWMVIGGYSVGL
                     AFPMIIAYALLWYYKVNQTIRRLLGVVGIPLLGFVSLYTGLLLSGAKFVPLWSQQYLP
                     YLFLNSGILTGLAAAGLAFLLFHTFVKTELDDMSRILQIVGYAILALIVVEGLELYLF
                     MNHLALNPEKLDETGKFINPNGGALAYEYVTQGVLAPWFWWGIVGMGLTFPLILTFIE
                     MIFDKIIRPYVNWVAGVKFASILAGGIILRFVIVWGGDLKAPLVFPPALFQLPLSG"
     gene            7818..9458
                     /locus_tag="EYO68_02860"
     CDS             7818..9458
                     /locus_tag="EYO68_02860"
                     /inference="COORDINATES: protein motif:HMM:PF13442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIC06777.1"
                     /translation="MIKTLKRYLHFLLVLVFLPASMAAESQEDIKIGEKIYTEQCVLC
                     HGPAGEGWDWEKKWSKPPFPVPNLLKVLPERSDEYLVTVIKLGGSAVGLTDFMPALGF
                     NLTDREVLNLVAYLRVLKPKPPADGSFFEKSAPPQPENLVWAPRMSRFLVQFQSPSII
                     AAALIRPAAFLYRRNPRTHRPFYPKLKEDIPLLAQQHSLGQNAGQHHTGSFPHAITLG
                     DFNSDGHSDFAIPASAARKLAVLLGDGKGGILSNHHYPVGQGPTWVTTADLNQDGHLD
                     LITTNTGAGSLSLYYGDGKGDFHDRKDIEEIHGTPCVVAGDFNQDGLPDLAVTQAKLS
                     KVVILTNDSTGIPKRTHAIDVERGPHIMDVVDLNQDEIPDLVVGSLGSHTLSIMIANG
                     KGTFEEANVVKVGRFPHYRAYGDWNGDGHVDAAVSVAGEESVEVLLGNGRGDLKTLAR
                     YSAGVNPHGLTSGDFNQDGKLDLAVSNRTSAEIVIFLGEGNGTFKKRPSIHTGHDGIA
                     LANGDFDEDGLIDLALVSASSNSLIFYRGDGTGNFHLW"
BASE COUNT         2622 a         2141 c         2552 g         2848 t
ORIGIN      
        1 catctgcagc gccggacagc ttactgcagg gggccgaatg ccttttttgt tttgaaaaaa
       61 taacagttct gatgtaactc gtgactttcg agtgatgtaa gcagtcaact ggaaatatag
      121 atagatcgtt ttctcttttt ctttcctctc aattatgtta accctttcaa aagtaacaaa
      181 acgttttggt tcgcaaaccg tcgttgacag cctggacctg agcattccgc aaggtcttgt
      241 ttttggactt cttggtccaa atggttccgg aaaaacaact ctgcttcgaa tggctatggg
      301 tttgcttcaa cctacttccg gacgaatcac catttttgaa aataactctc ccggagattc
      361 ggaagtgcgg aaaaaattgg gttacatgcc tcagcagctg gctgtttatc ccggattgtc
      421 tgtacgggaa aatgttttgt tttacggacg tttgtatgga attccagaaa acaatcttag
      481 cacccgctca ctggaaattc tggaaatggt agagcttgaa tcacatcaga acaaattagc
      541 aggcgatctt tccggtggaa tgcttcgcag gctgatgctg gcaacaacgc tcattcatca
      601 gccggctctg gtaattttag atgaacctac tgctggtgtt gatccgtcac tgcgcctgcg
      661 tttttgggaa tggtttgaac aattggcgca gggcggtgca tccattttaa ttacaaccca
      721 tcatatcagc gaagccggcc gctgtgacaa agtcgccttt ttgcgggaag gactattgct
      781 ggagcatgat acgccggata cactcatgcg acgttacaat tcggaaaatt tggaacaagc
      841 tttcgttcag gctactcaag caaagctgga ggattgaaat ggttgcacga tggatagcct
      901 ggcggattat tcgtgaaata ttgagggatc gtcgaacttt agcttttttc tttcttgtgc
      961 ctttgattgt catgtcactg atttactatg ctttgcagga agacgaaaca gcaaggctcg
     1021 gagttgtttc caggggagtg atgcgtctct ttgaagcaga tttgatgctg actctggaag
     1081 aagaggacat tgaactggtt tcacttgata ttccggatga agagattgat ccggaacgtt
     1141 tggaacaaat gatttggaca cagttacgca ccgggcaggc agagggtatt ttgtttttag
     1201 gagaacagtt attggaagag cgctttgatg gaaagcgtgg gaggattgat ttttatctgg
     1261 aaggttcacg tcctactctg acggcatcag ttgtagaatc agtcgcctct gttatggacg
     1321 atttagcatc ctctcttccg gtggtaattg acagttcatg ctcggcgttt tgcgccaact
     1381 cggtcaacat taagccgttg gatttggaag aacactacct ttacggctca gaagattatc
     1441 gtttgattga cttttttctg ccggtatttc cgccattttt tgtctttttc tttacatttc
     1501 tcatttcgac catcactttc cagcgggaac gagtacgtgg aacgctggaa cgactgttga
     1561 ttgctccggt ttcctttact caaattgtcc tgggttacgt aggaggcttt attatttttt
     1621 ccggatgtca ggccgccatc attttgacat ttattctctc gctgattggc ttcaaaatca
     1681 cgctcactca aattggtgca atagcttttt tgacctttct gatgctcctg atttcacttg
     1741 tactgggctt acttgcctca ttccttgcgg ccaacgagtt tcaggcgctg caattcattc
     1801 cgctcgtcat tttgccgcag atttttttaa gtgacatgat ttggagcatc gatggtttcc
     1861 ccaaaatttt ccagtggatt tcccttgctt tgcctttgac acacgcaaac accgctatga
     1921 gaaacgtctt actcaaacag cagaatatct gggattcatg gcctcaactt cttactttaa
     1981 gcggcttttt cttttgtatt ctcctgctgc tcgtcctcgt ctcccgccga catcaaaacg
     2041 cagcttgaac ttggtttcat ttcatcggac attttttcct gtttgagacg tattgaaaaa
     2101 atgatcttgt tcgttttgtg agtggcaagt taagcaaccc catatattgg tttttataca
     2161 ataaattttt gtctttgaat tactgtcagt agcctttttt tgtggccggt tttaatctat
     2221 tctcagtgtt ttttctttat gtcgaaaaaa aactcatttt ttccttgaca tgcttcccgt
     2281 gtagttgtac gttaactctc aggaagtgga agatgtggtt tttaaattca aattaacctg
     2341 ttttctcttt atgtcgaaaa aaaactcatt tttttcttga cattaatttg gggaatgaat
     2401 atcttacttt ccagagtaga aggtttggtc attaaatttt attataggag caaaaaccat
     2461 gattgaagga agaataaagc aacttgatct agctaaccgt tcagcattaa ttgttgaggg
     2521 aaatggcaat gaagttactg tccaatttgc gttgagaacc aacgtggagg tgatcgaacc
     2581 tgaaacggta ggcctaatgg gcggaaactt ggaagacctt gaagaaggat ttgaagttga
     2641 gattgaagtg gcctcgacag agaaagatgg aagcatcatt tgcgattcca ttgcttgcgt
     2701 cagctgatag atcagtctca ttagtatgga ttatccgctg ttacgttaca atggaacaca
     2761 ggctagactt tgactaaacg tctgctgctc cgtgttgggg tggttgattt gcagggccgt
     2821 tctggcaatt taatttagaa tactttctga atcagttagg agattaagat atggacaatg
     2881 aaaagagtgc gggtagaaga gggtttcttc tcggtgtgct tacagcgatg ggcgccacta
     2941 cggcgactgt gttggccgca cgtacatacg agacaaataa agatggagtg atagactcag
     3001 atgcttctac aggtccgatt ttgtaccaac ggactgagga aactgagcgc tattacaaaa
     3061 ctctatacaa ctaagccagg aggagtatga aacttaacag agtaatacaa aatgacgggc
     3121 aggacctgaa tcccgctttt atggacagtc tcaatcccag tgtgagtcgc cgcggttttg
     3181 tcaccgccgc cggaatggga gttggattga tggcgatggg tccctcattg gtaagagagg
     3241 caaaagcagc aacgggagcg gagactgcac ggaagctgac acagatcaag actatttgcg
     3301 gtaactgtgc tgtcggttgc ggtttcatcg ctgaggttga gaacgatact tgggtcagca
     3361 tcgaaccatg gttcgaacat ccaatcaacc agggtagcct gtgttcgaaa ggtgcagcag
     3421 cccgggaaca tgtaatcagc gataagcggc tcaagtcacc aatgaaactc gaagatggga
     3481 aatggaaacg aataagttgg gacacggcga tgagtgagat cacaaccaag ttgcaggaga
     3541 ttcgcaaaaa gcacggaccg gattccctga tgatccttgg ttcggcccac cacaataacg
     3601 aaacttcgta tgcgctgcgc aagttcgcag ccttctgggg ttccaacaac atcgaccatc
     3661 aggcccggat atgccattcc accacagtag ctggactggc caatgtgtgg ggctatggtg
     3721 cgatgaccaa ctccatgaac gacatccgca acagcaagag cgttctcatt ataggggaga
     3781 atgtctgcac ctcgcacccc attgcaatgc agcacatact cactgctaag gaagaaaatc
     3841 gcgcacaaat cattgttgtg gatcctcggt tttcgcagac ggctgccttc gctaacaagt
     3901 atgtacgaca tcgctcagga acagacgtgg cttttatcta tggtctggtg aacataatct
     3961 tgaagaatgg ttgggaggac aaacggatgc tccaggatcg gacttatggt ttcagcaacc
     4021 tgaaagagga actgaaacgc tacgatcctg aaacggtgtc cgacatcacc ggagtatcgg
     4081 tcgccgattt ggaacacaca gcgcggttga tcgctgaaaa cagacccggc acgctgatct
     4141 gggctatggg tggaactcag cacaccaatg gtacttcagt cacgcgttca tattgtgtgc
     4201 tgcagttggt actgggcaac atgggcaagc caggtggggg tacaaacgtg tttcgtggac
     4261 atgacaatgt tcagggggcc acagatcttg gtgtactgtc caatacgcta ccaggatatt
     4321 atgggttgtc caagggtgcc tacaaacatt ggtccaatgt atggggagtc gacaacaatt
     4381 ggatcaacgg ccgctttgcc aatgaaaaaa ttcagcacaa ggtaggtttc acagtagctc
     4441 gttggtacga aggagtgctg atggatccaa aggagcttgg acaggatgaa aatgtacatg
     4501 cggctttcta ttggggacac tccactaaca gtcaatcaca actggaccgt atcaaaaccg
     4561 ctctggacaa agtggaatta ctggtggata ttgacccgtt cgttaccacc acctccatcc
     4621 tgccagaccg caaggacggt gtttatattc ttccggctgc aacggtttat gagcagtctg
     4681 ggtctgtgac taactccaac agggacatcc agtggcgtaa ccaggttgtc aagccagttt
     4741 gggaatcacg taaagatctt gatattgtca ttgacctggc cgaacggctg ggttttggcg
     4801 acaagttcaa gaaaagcaaa aacggccctt ggaagcaatt gcccgaggat atcactcgcg
     4861 aatggaacct tggtatgcgc accattggga tgacaggtca aaccgtggaa agaatgaagc
     4921 ggcagcagga ttgggcacac gcctttgacc taaaaactaa aattgccgaa ggcggtccgg
     4981 ttgatggcga gcaatgggga ctcccatggc cttgctggac ggataaacac cctggcaccc
     5041 acattcttta tcgaacaagc attcctgtag cagaaggagg attgggtttc cgtgcccgct
     5101 ggggagtcaa ggctccggat ggcaatactt tgcttgcagg taagggtgca gcaccggttg
     5161 acagttcaat caatgaaggt tatgcggaaa tgaagaactg gaaaacagat ctgactggga
     5221 aagtgtttga agaggctatt gctaaagggc tggcccctta cggtaatggt cgcgcacgct
     5281 tcaacgtctg gacattcaaa gatgatccgg tgccaaaaca cagagaacct cttcacagtc
     5341 cgaggcctga tttgattact gaatggataa cctatgacga tgtgaaggat cactatcgcg
     5401 tacctactct ctacaaaagt ctgcagaaag ctgattgggt tgaaaaatat ccactgattc
     5461 tctcaaccgg aagacaagtc gaatttgaag gcggaggaaa cagcgaacga aactgctggt
     5521 ggttggtgga gttgcagcct gaaatgtatg ttgagatcca tccaagactg gccaatgata
     5581 acaggatcag acacggcgac tgggtctggg ttgaatcccc ggaggataca gacgggaaac
     5641 caagtcgtat caaagttaag gctaaggtca ccaggcgggt gagtccggat atggtctatg
     5701 tcccattcca ttggggggga gtaatgtccg gagtagactt gtcaaataaa tatcccgaag
     5761 gttatctccc atatggtatg ggggaatcgg caaatacaat aaccaactat ggttatgacc
     5821 gcatcactca gatgcaggaa accaaaaccg gtctttgcag aattaaaaag gcctgatttc
     5881 ggagaaggaa tatggcaaca acaaatatta ccgtccctac ccgtgcggtt tctggaccgg
     5941 aagggaaaca ttcgccggcc agaatgaagt tctatgtcga cgtcagacgt tgcattgagt
     6001 gtggcggttg tgaagtagcg tgtaagaacg agaacgaagt tccaccagga atagcacgca
     6061 tacgggtggt taccgtgaac gaaggacagg caggtgaaac aaatgtagca gttccctgca
     6121 tgcattgctc taatgcacca tgtgtttctg tttgtcctgt tgacgccttg tttaacaggg
     6181 cggatggtat tgttcacgtc aacaaggaaa cctgcatcgg gtgcggctat tgcctttacg
     6241 cctgtccttt tggggcaccg cagttcccca aaggttctcc ctttggcaag cgtggtgtga
     6301 tggacaagtg cacctattgt gcgggcggtc ctgaggaagc gttctcaacc cgggaacagg
     6361 aactttacgg atcaaaccgt attgcggaag gcaaacttcc catctgcgct tcgatatgtt
     6421 ccaccaaagc tttggtggca ggtgatgccg aagaagtggc caacgtggtt cgtagtcgtg
     6481 ctgcagctcg tggttcgtca agtggagctt ggggctggag caaagcttac aataattaaa
     6541 tggataagct cagagtaggt ccgtaaaatt tcaccggaga ttacggtctc cggtgaaaaa
     6601 tagggaaggt tatggtttgg tgttttggtt ttcgaggcat ctctcttcct ccggattgct
     6661 tttccggaca acatttgacg gatactcgcc ttacccccac ctgactgctt tcagtctttt
     6721 tacgacaccc taatttattc aaatggactt gtaatagtag gaatacaatg gaaccaatca
     6781 tcttgatgaa ctcaaaatgg gggaatgata tttttacccc catgtatcta tttttcggtg
     6841 ggctcacggg cgggttattt attgtggcag tggtggcaga tttagttggg ataaaataca
     6901 aaagctttga aaaattgtca aaagttgttg cttttatggt cccactcgca ctcgtattgg
     6961 caggcctgtt tatcaccttt caccttggaa aacctgaacg gggaatgttc tttccgcttt
     7021 atttctctaa ctttaattcg tggatggtga taggaggtta tagcgtgggt ttggcgttcc
     7081 caatgataat tgcttacgcg ttactctggt attacaaggt taatcaaact atccggcgcc
     7141 ttttgggagt tgtcggaata cccctgctgg gcttcgtgtc cttatacaca gggttactct
     7201 tgagcggagc caagtttgtt ccattatggt cacaacagta tttgccatat ttattcctca
     7261 attcaggtat cttgactggt ttagcagcgg caggtctagc attcttgttg tttcatactt
     7321 ttgtgaagac tgaactggat gacatgtcca gaatactgca gattgttggt tacgctattt
     7381 tagctctgat tgtagtggag gggttagaac tgtatttgtt tatgaatcat ctggcattga
     7441 atccagaaaa attagatgaa accggaaaat tcattaaccc caatggcggt gctttggcat
     7501 atgagtatgt tacacaggga gttttggcac cctggttttg gtggggtatc gttgggatgg
     7561 gtttgacatt tcctctgatt cttactttta ttgaaatgat ctttgataaa atcatcaggc
     7621 cttatgtaaa ctgggtcgca ggtgtcaaat tcgcttcgat tctggctggg gggatcatct
     7681 tacggtttgt gattgtttgg ggaggcgatt taaaggctcc gctggttttt ccaccggcac
     7741 tttttcaact tcctctcagt ggatagcatt gatgctctcc atgggaatgt tttccggacg
     7801 gcaccatttg ctaagtgatg attaagacct taaagagata tctccatttt ctattggtcc
     7861 tcgttttcct ccctgcctca atggcggctg aatctcagga agatataaag attggcgaaa
     7921 aaatctacac ggaacaatgt gtactttgcc atggtccagc aggagaagga tgggattggg
     7981 aaaagaagtg gtccaagcct ccttttccgg tccctaattt gctgaaagtg ctccctgaac
     8041 ggtctgacga gtatttggtt acagtgatta agcttggagg gagcgctgtc ggtttgaccg
     8101 attttatgcc tgccctcggc tttaatttga cagatcgaga ggttctgaat ctggtcgcct
     8161 atctacgagt attaaagccg aaacctccag cggatgggtc atttttcgaa aagtcagcgc
     8221 ctccccaacc cgagaatctg gtgtgggctc cccgaatgag ccggtttcta gttcagtttc
     8281 aatccccctc gataattgct gcagctctca ttcgccccgc agctttcttg tatagaagaa
     8341 atccccgaac ccaccggcct ttttatccga agctgaagga agacattcct ctgcttgccc
     8401 aacagcactc attgggacaa aatgcgggtc aacatcatac tggttctttc ccccatgcga
     8461 tcactcttgg agactttaat agcgacggcc actcagattt tgcgatcccg gcatccgctg
     8521 cgcgaaaatt agccgttctc cttggtgatg gaaaaggagg aatcctttcc aatcatcact
     8581 atcctgtggg gcaaggaccg acctgggtca ctacagccga tttgaaccaa gatggccacc
     8641 ttgatttgat cacgaccaat actggggcgg gcagcttgag tttgtattat ggagacggta
     8701 aaggagactt tcatgatcgc aaggacattg aggaaattca tgggactccc tgtgtggttg
     8761 caggtgattt taaccaggat ggccttccgg atttggccgt aactcaggct aagctctcca
     8821 aagtggtgat cttgaccaat gattcgactg gtatacccaa acggacccat gcgattgatg
     8881 tggagcgtgg tccacacatc atggatgtgg tcgacctcaa tcaggatgag attccggact
     8941 tggtggtggg aagtctgggc tcccatactt tatcgatcat gattgccaac ggtaaaggga
     9001 catttgagga ggcaaatgtg gtcaaagtcg gccgtttccc ccactaccga gcatacggag
     9061 actggaacgg tgatggacat gtggatgcgg ctgtctcggt cgcaggtgag gaatcggtag
     9121 aggtgctttt aggaaatgga cggggggacc tgaaaacgct ggcaagatat tcggcgggag
     9181 tcaacccgca tggcttgacc agcggggact tcaatcagga tggaaaattg gatctggcag
     9241 tttccaaccg aacctctgcc gaaatcgtca tttttctagg ggagggaaat ggaacattca
     9301 aaaaaaggcc ttccatccat accgggcacg atgggatcgc gttagcgaat ggcgatttcg
     9361 atgaggatgg gttgattgac ttggctttgg tttctgccag ttccaattcg ttgatctttt
     9421 acagaggaga cggaacagga aatttccatc tttggtaatg ccagttgcaa attattggct
     9481 cattttaata gtttggtgaa atccggtgtc cttccataag gacctgtttc ttcttgagtg
     9541 caagaaagat ttccccattt tggacttgtt ttgtaacact ctcagccact ctcctaagga
     9601 ctgaaatgtt cgtgacataa gcagtttcgt cctgccagtg ccttagcgac tctttagctc
     9661 tcgccttggt aatgtctcgc tggaatgcaa atgtgattgc agtttacctt tctgattgag
     9721 caacccagag tcaatagaag cttgtcgttt tttgttcccc gaatgtttat ttatcttggg
     9781 gcctaagata ttttaattat attttggcca cttaaaacta tctgttaaac tattgatata
     9841 aaaataattg ttgaaaaaac ttttagtgca ataataatgg atatatacta catgcattaa
     9901 gattgttcta aacgatgaat ttattgtaaa aaataactgc aagaaaatat gaggagctat
     9961 tttttccatt agggcagaga gaacacgtgg cgacacagtg accgtttccg aaaaacaaat
    10021 tttaggtccc catcagtgga ttgcagataa gtgagaagtt tcaattaaac agattgtgca
    10081 gacttgagaa ggtatctatg gatcatcgaa aaaaacaaat actatttttt tatcgaaagg
    10141 agagttcctg atgctggcaa agc
//
LOCUS       DTVD01000078            2560 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_16938_length_2560_cov_406.358483, whole genome
            shotgun sequence.
ACCESSION   DTVD01000078 DTVD01000000
VERSION     DTVD01000078.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16938_length_2560_cov_406.358483"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            390..>2560
                     /locus_tag="EYO68_02865"
     CDS             390..>2560
                     /locus_tag="EYO68_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIC06778.1"
                     /translation="MQRFLNFGGKMEQAIQIINLDIQGMTCAGCVNSVEKALGNVDGV
                     DLAEVNFALNRAAVHYNPEIANPSVLESAVEEAGFEAQRLKDTEDAPEPSEQSEQEYL
                     KFRSKMWLAFGFSVPLVLLAMGPMLGFSLPNWFAPETEPLRYGLIQLLLTLPVLWAGR
                     DFYTKGISTLLHRTPNMDTLVAMGTAAAVGFSLWNLFGTQLNAEGFYFETAGVIIALI
                     LMGKSLEAKSRSRASAAISSLLKLRPKEAVLVHEGKESSISIDLVHPGDVLRVRPGSI
                     VPADGIVVEGSSFVDESMLTGEPLPVEKTVGDHGSSGSEVTGGTLNTNGVLTIRVQRV
                     GGETTLSRIIRLVENAQLAKAPVARLADQVAGVFVPVVLVIAAFTGVGWWLSGASANE
                     ILGYTVAVLVIACPCALGLATPIAIMVGTGTGAQHGILFRNAPALEAAHRLDTLILDK
                     TGTLTEGRPKVTQIITVDTKNQKQAQTELLKLASAVEQGSEHPLGRAVVAEAEKQNLE
                     LPQISEFEAQTGFGVKAVCNGNLIFVGNPALMRSENIFSEVPEILAKQIPSGSTPIFV
                     ALDGNLAGVICLADEARPESAEAVRKFQQLGLEVVMLTGDQKTSAEAVAEKTGITKIH
                     AGVLPEEKSKIVKQYQARGEHVGMIGDGINDAPALAQADVGIAMGSGTDVVLETADMV
                     LMKNDLRHVADALRLSRATLRNIRQNLFWAFGYNVVGIPIAA"
BASE COUNT          680 a          520 c          681 g          679 t
ORIGIN      
        1 caccgcttac tggatgcgtc gtggtttttg tttttgaaga aataagttaa atggtgttct
       61 ccatcaatga aggttcattt aatttttaaa tagcgagcgg aagatttatc gcagtttgtg
      121 atgagtagcg ttgcgtgcaa gatgaggtcc cttaaaaagt gaagaaaaga aaaataaagc
      181 tgaaagacac aattttacct tcacatagtc tggaaaagtt tcaatgggaa aaataattat
      241 atttttagct tgacatgaac ataatacccc tataaggtat aaaagaatga tgaaacgata
      301 gggctttgct acgtctccac cttaggccgc cgtgtaatgc tgccctaccc ggtttatgtg
      361 ttcttttgga ttttgttata aaatcgaaca tgcaaagatt ccttaatttt ggaggaaaaa
      421 tggaacaagc gatacaaatt atcaatttgg atattcaggg gatgacttgc gccggctgtg
      481 tgaattcagt ggaaaaagcc ctgggcaacg tagatggtgt ggatttggcg gaagtcaatt
      541 ttgcgctgaa ccgggcagcc gttcattaca atccggaaat cgcgaatcca tcggttctgg
      601 aatccgctgt tgaagaagca ggatttgagg ctcagcgatt gaaggatact gaggatgcac
      661 cggaaccgtc cgagcagtcg gaacaggaat acttgaaatt ccgcagcaaa atgtggctgg
      721 cgtttggatt ttcagtaccg cttgttttgc tggccatggg gccgatgtta ggtttttcac
      781 ttcccaactg gtttgctccg gaaacggaac ccctgcgcta cgggctgatt caattgctgt
      841 tgactttgcc tgtgctttgg gccggacgtg atttttatac caaaggtatt tccacattgt
      901 tgcatcgtac ccccaacatg gataccctcg tagccatggg aactgctgcg gcggttgggt
      961 tcagtttgtg gaatttattt ggaacgcaac tgaacgcaga aggtttttat tttgaaacag
     1021 caggtgtgat catcgcacta attttgatgg gaaaatcgct cgaagccaaa agccgcagcc
     1081 gtgcttcggc agcgatcagt tcgttgttga aattgcgtcc caaagaagca gtcctggtgc
     1141 atgagggaaa ggaatcaagc atttcaattg atttggtaca tcccggagat gtcctgcgtg
     1201 ttcgtccggg aagtattgtg ccggcagatg gaatcgttgt ggaaggctca agttttgtgg
     1261 acgaatcgat gctcactggt gagccgcttc ccgttgaaaa aactgttgga gatcacggtt
     1321 ccagtggatc tgaagtcacc ggaggaaccc tcaacaccaa cggagtgctc acgattcgag
     1381 tacagcgcgt gggaggagaa acaacactgt cccgcataat ccggctggtg gaaaacgctc
     1441 aactggccaa agcaccggta gcacgtttgg ccgatcaagt tgcaggcgtt ttcgttcctg
     1501 tggttttggt aattgcggcc tttaccggag ttgggtggtg gctttccgga gcatccgcca
     1561 atgaaatttt aggttacact gtcgccgttt tggtgattgc ctgtccttgt gctttgggac
     1621 tggccacgcc gattgcgatt atggttggaa ccggaaccgg cgcacagcat ggaattttgt
     1681 tccggaatgc tcctgctctg gaagcagcac atcgactgga caccctcatt ctggacaaaa
     1741 ccggaacgct gaccgaaggc cgtccgaaag ttacgcaaat tataacagtt gatacaaaaa
     1801 atcaaaaaca ggcccagacc gaattattga aattagcatc ggcagtagaa caaggttccg
     1861 agcatccttt gggacgagct gtagttgctg aggcggaaaa acagaatttg gaattaccgc
     1921 aaatttcaga atttgaagcg caaaccgggt ttggtgtaaa agccgtttgc aatggaaatt
     1981 taatttttgt cggaaatccg gctttgatgc gaagtgaaaa tattttctcc gaggttccgg
     2041 aaattttggc aaaacaaatt ccttccggaa gtactccgat ttttgttgcc cttgatggaa
     2101 acttagcagg tgtgatatgt ctggcagatg aagctagacc tgaaagcgct gaagcggtcc
     2161 gaaaatttca acaattaggc ttggaagttg tgatgctcac cggtgatcaa aaaacttctg
     2221 cggaagcagt tgctgaaaaa acaggaatca ctaaaattca tgctggtgtt cttccggaag
     2281 aaaaatccaa aattgtaaag cagtatcagg cacggggtga acacgtgggc atgattggtg
     2341 acggcatcaa cgatgctccg gcattggcac aggcggatgt cggcattgca atgggatccg
     2401 gaaccgatgt ggtgctggaa acggcggaca tggtgctgat gaaaaatgat ttgcggcacg
     2461 ttgctgacgc attgcgactc agccgtgcga ccttgaggaa cattcgccaa aatctttttt
     2521 gggcctttgg ttacaatgta gtcggcattc cgattgctgc
//
LOCUS       DTVD01000079            2539 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_17155_length_2539_cov_231.245572, whole genome
            shotgun sequence.
ACCESSION   DTVD01000079 DTVD01000000
VERSION     DTVD01000079.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17155_length_2539_cov_231.245572"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            176..1408
                     /locus_tag="EYO68_02870"
     CDS             176..1408
                     /locus_tag="EYO68_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HIC06779.1"
                     /translation="MTLSNLRINIDRLVASIEDLAEIGAIDGGGVCRMALSDEDRQGR
                     DLVVGWMRKLGLDVTIDQLGNVIGIRAGLESGKPVMTGSHIDTVASGGRYDGNLGVLA
                     GLEVIATLNEAEITTRKPIGVGFFTNEEGSRFAPDMMGSGVHQGAFDIKDMLDVVDID
                     GARVGDELEKIGYAGDTSTGDFRADSYVELHIEQGPILELEDYEIGAVEGVQGISWNE
                     YKISGMANHAGTTPMRLRHDAGYVAAAISVEARRLANEMGANQIGTVGVTKLNPGLIN
                     VIAREALITIDLRNTDEQKLQEAEHTMTSFIEQICKAEGVTCSSRTLARFEPVSFDKE
                     MVSLVSNIASTLGNRVKSMPSGAGHDAQMFAPNCPTAMIFVPSRKGISHNVAEFTEPR
                     HLQAGADVLLQILLQKSA"
     gene            1423..2361
                     /locus_tag="EYO68_02875"
     CDS             1423..2361
                     /locus_tag="EYO68_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HIC06780.1"
                     /translation="MSRILKVAAAQLGPIALDEPRSSAVGRMIVLLQNAKAQGAELVI
                     FPELALTTFFPRWYTEDQSEIDKYFETEMPNKDTEPLFAEARKLKIGFNFGFAELVVE
                     KRVTRHFNTAIIVDQQGRIAAKYRKIHLPGHTENEPWRAFQHLEKRYFEKGNLGFQVH
                     QVFGGKIGMCICNDRRWPETFRVMGLQGVELVALGYNTPAHYPLAPEHDHLQDFHNHL
                     VLQAAAYQNGTWIVGVAKAGREEDCDLIGGTCIIAPTGEIVAECKTLADEVIIAECDL
                     DRCKEIQENIFNFDLHRQPEDYALITAPKKESASNG"
BASE COUNT          759 a          491 c          635 g          654 t
ORIGIN      
        1 gtagcaaacg ccttttttca attcaacggc gaacgtcaat atgagttgcc attgaaacag
       61 gtcaaatgac taaaaaataa aagtctttta ttattccata caataattaa taaatgtggg
      121 tgtggttgaa agtttggcca cgccctccgg atgaaaataa agttaagtta ttaaaatgac
      181 attatctaat cttcgtataa atatagaccg tcttgtagca agcattgaag acttggctga
      241 aattggtgca atagatggcg gcggcgtatg cagaatggcg ctcagcgatg aagaccgcca
      301 aggccgtgat cttgttgttg gatggatgcg taagctcggg ctggacgtca caatcgatca
      361 attagggaac gttatcggta taagggcagg tctggaatcc gggaagccgg taatgaccgg
      421 atcacatatt gatacggtag cgagcggagg acgttatgac ggtaaccttg gggttctggc
      481 tggtttggaa gtaatagcga ccctgaatga agcagaaatt acaacacgaa aaccaattgg
      541 ggtaggtttt tttacaaacg aagaaggatc ccgttttgct ccggacatga tgggaagtgg
      601 agttcatcag ggagcttttg atattaaaga tatgctggat gttgttgaca ttgatggagc
      661 acgagttggt gatgagcttg aaaaaattgg ttatgccggt gatacatcaa caggtgattt
      721 tcgtgcagat agttatgttg aactgcatat tgaacaaggc ccaatactgg agttggaaga
      781 ctatgaaatc ggggcggttg aaggtgttca agggatttcg tggaatgaat acaagatcag
      841 tggtatggca aaccatgccg ggacaacacc gatgcgactg cgacatgatg caggatatgt
      901 ggcggcggct atttcggtgg aggcacgccg ccttgcaaat gaaatgggag ctaatcaaat
      961 cggaactgtc ggggtaacaa aactaaatcc cggtttgatc aatgtgattg ccagggaagc
     1021 actaattacc attgatttgc gtaataccga cgaacaaaaa ttacaagaag ctgaacatac
     1081 aatgacctca tttattgaac agatttgtaa agcagaaggg gttacatgtt caagtcgaac
     1141 tctcgccagg tttgaaccgg tttcttttga taaagaaatg gtttcattag tttctaatat
     1201 agcatcgaca cttggtaacc gggtaaaatc gatgccgtct ggggccgggc atgatgcaca
     1261 aatgtttgcg ccaaattgtc caacagccat gatcttcgtg ccaagtcgaa aaggcattag
     1321 tcataacgta gccgaattta cggaacccag gcatcttcaa gccggagccg atgtgttgtt
     1381 gcaaattttg ttgcaaaaat ccgcataaac aaaaattact ggatgtccag aatcttaaaa
     1441 gtagctgctg cgcaacttgg tccaattgct ttagatgaac ctcgctcctc agctgtcgga
     1501 cgcatgatag tcctgttgca aaatgccaaa gcacaaggtg cggagctggt tatctttccg
     1561 gagctggcac tcacgacttt tttcccgcgt tggtacaccg aggatcaatc cgaaattgac
     1621 aaatacttcg aaaccgaaat gccaaacaag gatacagagc ctttgtttgc tgaagcacgg
     1681 aaactaaaaa taggctttaa tttcggtttt gcggaacttg ttgtggaaaa aagagtcact
     1741 cggcatttca atacggcaat tattgtcgat cagcagggcc ggatagcagc taaatatcgg
     1801 aagatacact taccgggaca cacggaaaac gagccatggc gcgcttttca acatctggag
     1861 aaacgatatt ttgaaaaggg taatttaggt tttcaagttc atcaggtgtt tggcggcaaa
     1921 attggcatgt gtatttgtaa tgaccgccgt tggcccgaga cttttcgggt catggggctg
     1981 caaggagttg aacttgtagc gctgggttat aacacaccgg cacattatcc actggcaccc
     2041 gagcatgatc atcttcagga ttttcacaac cacttggttt tacaagctgc cgcatatcaa
     2101 aatgggacct ggattgttgg tgttgcaaaa gccggaaggg aagaagactg cgacctgatt
     2161 ggaggcactt gtatcattgc accaacaggt gaaattgttg ctgagtgtaa aaccctggca
     2221 gatgaggtga ttattgctga atgtgacttg gatcgctgta aagagataca ggaaaacatt
     2281 ttcaattttg atcttcatcg ccagcctgaa gactacgcct taattacggc accaaaaaaa
     2341 gaatcagcct caaatggtta aaggtcagtt ccaagaatga tgtaaccgaa aaaaatccac
     2401 aaaagcaaaa gatcgctaca gagtgtcaat gcggacaagt agtaacgctt cagatttaga
     2461 tatctttttt aaaatccccg caacggcatt tccggtgaac gctgcagcca gttccactcc
     2521 ggataatctg cggtacctt
//
LOCUS       DTVD01000080           10068 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1725_length_10068_cov_332.517427, whole genome
            shotgun sequence.
ACCESSION   DTVD01000080 DTVD01000000
VERSION     DTVD01000080.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1725_length_10068_cov_332.517427"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..3016
                     /locus_tag="EYO68_02880"
     CDS             <1..3016
                     /locus_tag="EYO68_02880"
                     /inference="COORDINATES: protein motif:HMM:PF03176.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIC06781.1"
                     /translation="GDTTGLQSETETPEDTAPDCSLVNSGESSEKLRVEAETEINKIV
                     SSLKNHPLFEGDVLSKNLSTAALIITFRPGSKPESDNTQTILKGLLSKYQKDSEIAET
                     LRIAYAGQPRQINKASRLIRQDMALILPLSLMLIVVVLLIAFRSIKAVIVPLSVVLFG
                     ILWTAGIIGLIGDELNLVTMACAPIIVCVGSAYVIKFLNQYQIESLQIREAKKSGVPQ
                     PTIPEVVYATLSSVTVPVTVTAITTVAGFIALVVSPIPAVQQLGLYSSVGIISINLFT
                     LTLAPALLHFIRLPDLAPTENQSGLLDRFFQVIVEWLRLYSKRLIWIWLTVAAFAALG
                     MLDLSINSSTKTFPEDSQLVKDLQLIEDELSGTDTLRLLFRAGNESENQQDFSYNPLK
                     TAKTIFGLKELQDWLFQVKDVTEIGNIEGLRIDKMHSPVDVLEHYRMGLDKLSDKEVV
                     QFFDKTAENGPKFLSDAEDVMQVTLRMRISGSTAFLALRDLLLQKVPQLLPHLQFSYT
                     GGGVLSSESANNIAQGQINSVFLALVIVFVILSMLFLSWKMGVIALFPNVITILIFFG
                     SLGWLNIPIGVTISVIAAIALGIGVDDTIHFLSHYNENANKLRNKRKASLKTLPIVAR
                     PMLFSTIALSAGFILFALSEMESQVLFGTFTAFTLLVCLAIDMTYLPSVVMETGLITV
                     WDYVGLKFDEQFIEGIDLFQNMTVREAKIASLMAYTEDLEKGTLLFTQGEIGDEMYVV
                     LSGSISIFLEKNGKRTDLVRLEKGNTFGEMGLFRKAERSASAEAAEKTRLLVVNRDCL
                     EPLKKRNPKIAAKLFLNLANRLQSSLKETDERLLTQKEFDLTSLEAKLNANENLEQTE
                     ISIEPAEFWEKLGKKWRRIIMSYCESHSIMSGKKITKIKAKKGDYIFITSGEVEIEST
                     VSAKSETFSVGYCWTRKDFDLIGEFSLCTGNADSAARAIARKDSTLLQFTETELLSLA
                     QHEPRIAVQFLEDLVCILSDQLAIADKRLQDH"
     gene            3146..3526
                     /locus_tag="EYO68_02885"
     CDS             3146..3526
                     /locus_tag="EYO68_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC06782.1"
                     /translation="MPAERDMKILVVDDFAATRKILINTLAQLGYNNTDEADDGYSAL
                     VKLKSAIFDFVVLEWNMSKMSGMELLEQIRAVPNLKQIPVLMVTEDVLQENIVAAVKA
                     GLNAYVIKPVEVNNFAKKIEKIFE"
     gene            complement(3592..3870)
                     /locus_tag="EYO68_02890"
     CDS             complement(3592..3870)
                     /locus_tag="EYO68_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIC06783.1"
                     /translation="MSVQKRISLVITGKVQGVGYRYSVKLKAESLAIRGYVRNQLDGS
                     VFVTAQGETTAVENFVKWCYKGPPAAFVRGIEKTPGSIEDFSEFSILY"
     gene            complement(3890..4861)
                     /locus_tag="EYO68_02895"
     CDS             complement(3890..4861)
                     /locus_tag="EYO68_02895"
                     /inference="COORDINATES: protein motif:HMM:PF01656.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06784.1"
                     /translation="MHRTIIPIASGKGGVGKSFIAANLAIALAKLGKQVIVADLDFGG
                     SNLHTCLGIHNTNPGIGDFLRARYSKLKDLLVETENENLKFLAGDVRSPFLANMHHSQ
                     KMRLINNLRQLECEYLILDLGSGSTYNTLDYFGMTQLGMIITTAEHASTVNMLTFLKL
                     FAFRVIERSMPKNTFLDEKLRDSYNRTVMDEVLTVRGLLDEISTIDRNVAQKAEQSWI
                     RYRPRIIINKCNLPNELDLLGSLENTFNQILMLRSDFFGCLFTDPAVSKTFKESKTLN
                     VAAPNSDILEDINLLADRVTRLWEQPIRNSANLLQFNAKNLFEKRQK"
     gene            complement(4986..5456)
                     /locus_tag="EYO68_02900"
     CDS             complement(4986..5456)
                     /locus_tag="EYO68_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC06785.1"
                     /translation="MSVRIDFAHSPADFDNARKLFIDYAESLGFSLSFQGFEDELEHL
                     PGKYAAPEGCILLAWDELDCVGCAGLRPLSDDVCEMKRLYVKPLYRGTGLGRLLAEKI
                     VQLGIDKKYTRMQLDTLNSMQSAVGLYKSLGFVETDQYYNNPHPEVVFFELTLD"
     gene            5934..7832
                     /gene="htpG"
                     /locus_tag="EYO68_02905"
     CDS             5934..7832
                     /gene="htpG"
                     /locus_tag="EYO68_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIC06786.1"
                     /translation="MSKKAKPKPEVHEYQAEMQKLLEILVHSLYTEREIFLRELVSNA
                     SDALSKVQLTTLTEEKVLDKDTELQITISLDEEQRKIVIEDSGCGMTKEELVENLGTI
                     ANSGTLKYLEQLQTENKSAENLIGQFGVGFYSVFMVADRVELTTRSWQEDAKAWQWSS
                     EGGGQYEIIPVDFKQRGTRVTVFLKEDAKEFCSEWRLESIIKKYSNYLPFPVKVKDKT
                     INQVKAIWTQSKSEVKDDEYKEFYKQISHGQQDPFHHLHFNIDAPIQYYALLYFPESV
                     GNEVLYARESKDIALYAQKVLIQTGNTDLLPSYLRFVNGVVDSEDLPLNVSRENVQKN
                     VLIEKIKNSLTLRVLKELQTLAEQNVEKYGKFWQTYGTLLKEGVSSDFKNKDRLKDLL
                     RFNSSISEDSSTDVTLKDYVSRMREGQKDIFYVTGGSREAILHNPNLEYFRRQGLEVL
                     FLTDQIDDFMVVDLRDYDGKTLKNISQGDIEGINDSDMSGAESSLSKEEKSDILAFFK
                     KQLKDRVSEVSDSKRLVDSPCSLVTPKDGMTAQMEKMMKMMDQKFQGEKRNLEVNMGH
                     PIIQNLSEILQKNKKHPFLEETVEQLYENSLLVEGLLENPVQILSRYQMFMESASAYE
                     LEKLNRVE"
     gene            complement(7912..8394)
                     /locus_tag="EYO68_02910"
     CDS             complement(7912..8394)
                     /locus_tag="EYO68_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06787.1"
                     /translation="MTLINTLNHILRHYYRVFFDSFPSKLPSDLQIYQNDCEDLVGRY
                     NILLKKISEYMKDHCKQQEDMPYNSDLIFSYHDTDSFFLISIKEDLENLTDDCEELLE
                     KGELHPSEIQLHNDAIEQNINKLNELRQYSEQIETALHEYEENIMKIEEEISNNTLCN
                     "
     gene            8508..9377
                     /locus_tag="EYO68_02915"
     CDS             8508..9377
                     /locus_tag="EYO68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HIC06788.1"
                     /translation="MAISMTGFGAADAQWETWICQVEIRSVNQRFLDIRCRLPFGFQT
                     MEPEIKKQIKAVSSRGKIDCSIKLEKGASEEKLKLNLERAQHYNELLTEFEALSGRKV
                     GVDARDLSSLNIIEENKSGEPPEKCEKVIRECLSRALEGLQEMKEREGQAMQSDIQQR
                     LSSCGNIVNSIEKLSQAEPERFRDRLQERFSQLTEGVEINPERLEQEIALLADRLDIS
                     EEVVRFRTHLEHMDDILSQHEVGKKAEFLMQELNREVNTMASKSNHAKISQASVEIKS
                     ELEKVREQLQNIE"
     gene            9385..9939
                     /locus_tag="EYO68_02920"
     CDS             9385..9939
                     /locus_tag="EYO68_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase"
                     /protein_id="HIC06789.1"
                     /translation="MKITLAGSPGSGKSTLRRMLAEHYGLKTKGTGDFMRELSRKHGY
                     SDITQFLVEYVSEHPEVDHQVDEEQRLYGEQNEDFVLDAHLGFYFVPDSIRICLICDL
                     EEAARRILEDTERTTEEAKTIRESIDTSQKRRDTMRRNFLSLYKVDIDDRSNFDLLMD
                     TSSLSSAEVFERVSTFIDEQTSNI"
BASE COUNT         3164 a         1824 c         2139 g         2941 t
ORIGIN      
        1 aggtgataca actgggttac agtctgaaac agaaacgcct gaggacacgg ctccggactg
       61 ttcgcttgtt aactccggtg aaagttctga gaaactgcgg gttgaagcag agactgaaat
      121 caacaaaatt gtcagcagcc tgaaaaatca ccccctcttt gaaggagatg tgctttccaa
      181 aaatttgagc acagccgcac tgataattac cttcagaccc ggatcaaaac ctgaatccga
      241 caacactcaa acaattctga aagggttgct gtcaaagtac cagaaagatt ctgaaatagc
      301 tgaaactctt agaatagctt atgcgggaca accaaggcaa attaacaagg ccagccgttt
      361 gattcgccag gatatggcac ttattcttcc tttaagtttg atgctgattg ttgttgtgct
      421 tttaattgca tttcggagta tcaaggctgt aattgtgcca ctgtcagttg tcttattcgg
      481 aattctctgg acggccggaa tcatcggttt gattggagac gaacttaatt tggtaaccat
      541 ggcctgtgcg ccgataattg tttgtgtcgg cagtgcttac gtgatcaagt ttctcaacca
      601 ataccagata gaatctctgc agatccgaga agcaaagaaa tccggggtgc ctcaaccgac
      661 aattccggaa gtagtgtatg caactctttc ctccgtaacc gtccctgtta cagtgacagc
      721 catcaccaca gtcgcaggtt ttattgcact tgtagtgagt ccaataccgg ctgttcagca
      781 actgggactg tattcaagtg taggtataat ttccatcaat ttatttactc tcacattagc
      841 acctgcttta ctgcacttca tccgtctgcc ggatttagca ccaacggaaa atcagagcgg
      901 actgctcgat cgcttttttc aagtaattgt agaatggctt cggctgtatt ccaaacgttt
      961 gatttggatt tggctgacag ttgcagcttt tgcggctctc ggcatgttgg acctgagtat
     1021 caacagttct acaaagactt ttccggaaga cagtcaactg gttaaggact tacagttgat
     1081 agaagatgaa ttgtccggca cagatacgct gcgtttatta ttcagggcag gaaatgaaag
     1141 tgaaaatcaa caagattttt catacaatcc tttaaaaacc gcaaaaacca tttttggact
     1201 caaggagctt caggattggc tttttcaggt aaaagacgtt actgaaattg gcaatattga
     1261 aggacttaga atcgataaaa tgcattctcc tgttgatgtc ctcgaacatt acaggatggg
     1321 cctggataaa ttatctgaca aagaagtggt tcagtttttt gataaaaccg ctgaaaatgg
     1381 tccaaaattc ttaagtgatg cagaagatgt gatgcaggtt actttgagaa tgcgcatcag
     1441 cggttccact gcttttctgg cactgaggga tttgctgtta caaaaggttc ctcaactgtt
     1501 gcctcattta cagttcagct atacaggcgg aggtgtgctc tccagtgaat ctgcaaacaa
     1561 cattgctcag gggcaaataa acagcgtttt tcttgccttg gttatagtat ttgtcatttt
     1621 gtcaatgctt ttcctttcct ggaagatggg tgttatcgcc ttgtttccga atgtaataac
     1681 aattttgata tttttcggtt cacttggttg gctgaatatt ccaattggag tgacgatttc
     1741 agttattgcc gcaatagcac tgggaattgg agttgacgac acaatccatt ttcttagtca
     1801 ctacaacgaa aatgccaata aactacgtaa caaacgaaag gcatcattga agacgttacc
     1861 gattgttgca cgtccaatgc tattttctac tattgctctt tcagcaggat tcattttgtt
     1921 tgcactatca gaaatggaat ctcaagtttt gtttggtact tttacggcat tcacattact
     1981 cgtttgtctg gcaattgata tgacatattt gccttcagtt gtgatggaaa cagggctgat
     2041 aacagtttgg gattatgttg ggcttaaatt tgatgaacaa tttatagagg gaattgacct
     2101 gtttcagaac atgactgtca gggaagctaa aattgccagt ctgatggcct acacggaaga
     2161 cttggaaaaa ggaactttgc tcttcactca gggtgaaatt ggtgacgaaa tgtatgtggt
     2221 tttgagtggt tcaatatcta tcttcctgga aaaaaatgga aagcgtactg accttgttcg
     2281 tttagaaaag gggaatactt ttggtgaaat gggattgttc aggaaggcag aaagaagtgc
     2341 aagtgcagaa gccgcagaaa aaacaagact actggtagtt aatcgtgact gcctggaacc
     2401 tcttaaaaag cgtaatccaa aaattgcagc caaactgttt ttgaacctgg ctaatcgtct
     2461 tcaatcgtcc ttgaaggaga cagatgagcg gttgcttaca caaaaagaat ttgaccttac
     2521 aagccttgag gccaagttaa acgcaaatga gaatttagaa cagacagaga tatcgattga
     2581 accagcagaa ttctgggaga aacttggtaa aaaatggcgc agaataataa tgtcttattg
     2641 tgaatcgcac tctattatga gtggcaaaaa aataacaaaa attaaagcca aaaaaggtga
     2701 ctatattttt attacttcag gagaagtgga gattgaatca acagtttcag caaaaagcga
     2761 aacattttct gtgggctatt gctggactcg aaaagatttt gatttaattg gagaattttc
     2821 actatgtaca ggaaatgccg actctgctgc acgggcaatt gcaagaaaag attcaactct
     2881 tctgcagttt actgagaccg agcttttatc tcttgctcaa catgaacctc gaattgccgt
     2941 tcaattcctt gaagacttag tctgcatcct ttcagaccag cttgcaattg cagataaacg
     3001 tttacaggat cactaaacaa ctattaattc tattgcaccc attaaagctg ctgagattta
     3061 gttttctgtc atgcagcatc acttgaaaaa ctgaaaagct gttgatatgt taagtttgat
     3121 acatttagag tcgttgtaaa ggagtatgcc ggctgaaaga gatatgaaaa ttcttgttgt
     3181 tgatgatttt gcagcaacgc gaaaaatttt aattaatact cttgcacagc tgggttacaa
     3241 caacacggat gaggcagatg acggttactc ggccctggtt aaacttaaat cagctatttt
     3301 tgattttgta gtcctggaat ggaatatgtc aaaaatgagt ggaatggagt tgctggagca
     3361 aatacgcgct gttccaaacc tcaaacaaat tcctgtgtta atggtcaccg aagatgtatt
     3421 acaggaaaac attgttgcgg cagtaaaagc cggtttgaat gcttatgtaa tcaaacctgt
     3481 tgaagtaaac aattttgcaa aaaaaattga aaaaatattt gagtaagagt cgattatttt
     3541 acttgtttct caaatcccca tgcagaatca cgaagggttt ggagaggtta gttagtagag
     3601 aatgctgaac tcgctgaagt cttcgataga tccaggagtc ttttctatac ctctaacgaa
     3661 tgctgccgga ggtcccttat agcaccattt cacaaaattt tctactgcag tagtctctcc
     3721 ttgcgctgtc acaaaaaccg acccgtcaag ttggttcctg acgtagcctc tgattgccag
     3781 agattcagct ttgagcttca cggaataacg gtatccgaca ccttgcactt tcccagtaat
     3841 gaccaaactg atgcgctttt gaacggacat tatttacact gcgtcctaat tatttttgcc
     3901 ttttttcaaa aagatttttt gcattaaact gcagcaggtt agcactattt ctaattggtt
     3961 gttcccacag gcgggttact cggtcggcaa gcaggttaat gtcttcaaga atgtcagaat
     4021 tgggggccgc gacattcaac gtcttacttt ccttaaaggt ttttgaaact gcaggatcgg
     4081 taaaaagaca accaaaaaaa tcacttctaa gcattaaaat ttgattgaaa gtattttcca
     4141 atgatccaag taaatccaat tcgtttggca aattacattt gttgattata atcctaggtc
     4201 tgtaacgaat ccaactctgt tcggcttttt gtgctacatt cctgtcaatt gtagaaattt
     4261 catccaacag accccgtacg gtcaggactt catccatcac agtgcggttg taagagtccc
     4321 tcaacttttc gtcaaggaaa gtgttttttg gcatagaacg ctcaattact ctaaaagcaa
     4381 aaagtttgag gaaagtaagc atgtttactg ttgaagcatg ctctgcagta gtgataatca
     4441 tccccaattg tgtcattcca aaataatcca aggtattgta agtacttccg cttccaaggt
     4501 ccaatataag atattcacat tccaattgtc tcagattgtt aatgagtcgc attttttgtg
     4561 agtgatgcat gtttgccaga aacggacttc taacatcacc agcaagaaac ttgagattct
     4621 cattttctgt ttctacgagc aaatctttca gtttgctgta tcgtgcgcgt aaaaaatctc
     4681 cgatgccagg attagtattg tgtattccca gacaggtatg aagatttgat cctccgaaat
     4741 caaggtctgc aacaataacc tgtttgccaa gtttcgccaa tgcaattgcc aagttagctg
     4801 ctataaagct tttaccaact ccaccttttc cacttgcaat tggtataata gttctatgca
     4861 tagctgatcc aagtttagtt attaaaagta taaatcaaat acgtcaggtc caatggagac
     4921 aggataacca agaaaaaaca taaaagcaaa taaaagaatt ttctgcaata ttaagctact
     4981 gtcaattaat ccagagtaag ctcaaaaaac actacttccg gatgtggatt gttgtaatac
     5041 tgatctgtct caacaaaccc aagagactta tagagtccaa ctgcggactg catgctgttt
     5101 aaggtgtcca actgcattct ggtatatttt ttatcaatac cgagttgcac aattttttca
     5161 gccagcaatc gccctaaacc agtaccacgg tataatggtt taacgtaaag acgtttcatc
     5221 tcgcatacat catcacttaa aggccgcaaa cctgcgcaac ctacacaatc caactcatcc
     5281 catgcaagga ggatacagcc ttcaggtgca gcgtattttc cgggaagatg ttccagttcg
     5341 tcctcaaagc cttgaaaaga cagactgaat cccaatgatt cagcataatc tatgaacagc
     5401 tttcgagcat tgtcaaaatc agctggacta tgagcaaagt cgatccgaac agacatttta
     5461 taaattatac atgaagacta gttcaaaatg agttgtcatt tcagtacatg cagcaatacg
     5521 ggatttgtaa gaatctgaaa aatttgtcct cagcctgcat caaacactgt catgagttcc
     5581 caaatttaaa atgaattaaa ataatagtca aaatttttgt ttgtctgaac tcaatatagt
     5641 ctattttacg taatccaaat atgattctta aacccaccaa ataaaaaaac gaacacaatc
     5701 aacaaatagg gatgccatcc aaagtaatgg taaattcggt tgcggtgagt aaagagtact
     5761 gaaggctgac acagttatca gcaaaagaat atttaaagaa ctgttattaa tgaaaaaata
     5821 aagctaactg tgtgttgaat attattaaat gacttttcat tttgcaagtt tttaagtagc
     5881 attgaaggtg aataaaataa ttacagcaac atttgtcatc aaggaaaaac tttatgagta
     5941 aaaaagcaaa gccaaaacca gaagtccatg aatatcaggc tgagatgcag aagcttctcg
     6001 aaattctggt acattcatta tacacggaac gagaaatctt tctccgtgaa cttgtctcaa
     6061 atgcatctga cgcactgagc aaggttcaat tgactacatt gacagaagaa aaagttctgg
     6121 acaaagacac agaactccag atcacaattt ccttggatga agagcagcga aagattgtta
     6181 tagaagactc cggttgtgga atgacaaagg aagaattggt tgaaaatttg ggaacaattg
     6241 caaattccgg aacgctaaaa tatttagagc agttacaaac tgagaacaaa tctgcagaaa
     6301 atctgattgg acagtttgga gttggtttct attcagtttt catggtagca gatcgtgtgg
     6361 aattaaccac ccgttcctgg caagaagatg ctaaagcctg gcaatggtct tccgaaggag
     6421 gaggtcaata tgaaattatt cctgttgatt ttaagcagcg tggcacgcgt gttactgtgt
     6481 ttctaaagga agatgccaag gaattttgca gtgagtggcg tcttgaatca attataaaaa
     6541 aatactctaa ctatcttcct tttccggtaa aagttaagga taaaacgatc aaccaggtaa
     6601 aagcgatttg gacgcagtca aaatctgaag tgaaagatga tgaatataaa gagttttaca
     6661 agcagatttc tcatggtcag caggatccgt ttcatcattt gcattttaat attgacgccc
     6721 caattcagta ttatgccctg ttgtatttcc ctgaatcagt aggaaatgaa gtgctttatg
     6781 cacgagagag caaagatatt gcgctttatg cgcagaaagt tttgattcag accggcaaca
     6841 cagacctcct tccatcatat ttacgttttg tgaatggggt tgtggattca gaggatttgc
     6901 cgctgaatgt gtcccgggaa aacgtacaaa aaaatgtgct gattgaaaaa attaaaaata
     6961 gtttaacttt gcgtgtgttg aaagagttgc agacactggc agaacaaaat gttgaaaagt
     7021 acggaaaatt ttggcaaaca tacggaactt tgcttaaaga aggagtttct tctgacttta
     7081 agaacaaaga cagattgaag gacctgttgc gctttaactc ttccatatca gaagattctt
     7141 caacggacgt gactcttaaa gattatgtca gcaggatgcg tgaaggacaa aaagacattt
     7201 tctatgttac cggaggttcg cgtgaggcaa tattacacaa tcccaatctg gaatattttc
     7261 gcagacaggg cttggaagtt ttgtttctaa cagatcaaat tgatgatttt atggttgttg
     7321 atctaaggga ttatgatggg aaaactttaa aaaatatatc ccagggagat atagaaggaa
     7381 ttaatgacag tgacatgtct ggagcagaat cttcactttc caaggaagaa aaaagtgata
     7441 ttcttgcatt tttcaaaaaa cagcttaagg atcgtgtcag tgaggtctct gactcaaaac
     7501 gcctggtgga cagcccctgt tctctggtga ctccaaagga tggaatgact gcacaaatgg
     7561 aaaagatgat gaaaatgatg gaccagaaat ttcaggggga aaagcgtaac ctggaagtca
     7621 atatgggaca cccgataatt caaaatcttt ctgagattct acagaaaaat aaaaaacacc
     7681 cttttctcga agaaactgtg gagcaactct acgaaaattc attgttggtt gaaggtttac
     7741 tcgaaaatcc agtgcagatt ttgtcccgtt atcagatgtt tatggagtcg gcaagtgcgt
     7801 atgaattgga gaaactcaac agagtagagt gagaaaggat ttttggggag atccgataaa
     7861 ctgttggaag aaaaatgatg gtgggcagtc gtttggactg tgagaattat tttagttgca
     7921 caatgtatta tttgagattt cttcctctat tttcattatg ttttcttcat actcatgtag
     7981 agctgtttca atctgttcgg aatactggcg cagttcatta agcttgttta tattttgttc
     8041 gatagcatca ttgtgcagtt gaatttctga tggatgcagt tcaccttttt ccagtaattc
     8101 ttcacaatca tctgtaaggt tttccaaatc ttctttgata gatatcagaa aaaaagaatc
     8161 agtatcatgg taggagaaga tcaagtctga attatatggc atatcttcct gctgtttaca
     8221 atgatctttc atgtattctg aaattttttt cagtaaaatg ttgtaacgtc caaccaggtc
     8281 ttcacagtca ttttggtata tttgaagatc actgggcagt ttagagggga atgaatcaaa
     8341 aaacactctg taatagtggc gcaggatatg attaagcgtg tttatcaaag tcatcagtct
     8401 tttggcaagt aattaaattt taacctgtaa tataacttga atagagcgat ttgtcaatta
     8461 gagaagtagt ataatgctta aatcaattat cagtttataa ggaataaatg gcaatttcaa
     8521 tgacaggatt tggtgcagca gatgcgcagt gggaaacatg gatttgccag gtggaaatac
     8581 gctctgttaa tcagcgcttt ctggatattc gctgtcggct gcctttcggt tttcaaacta
     8641 tggaacctga aattaaaaaa cagatcaaag ctgttagttc acgcggaaaa attgactgtt
     8701 caatcaaatt ggaaaaaggt gcctcagaag agaaactgaa acttaatctg gaacgtgcac
     8761 aacattacaa tgagttattg acagaatttg aagctttgtc tggaagaaaa gttggtgttg
     8821 acgcacgaga cttatccagt ttaaatataa ttgaagaaaa caaatccggg gaaccgcctg
     8881 aaaagtgtga aaaagtgatc cgggaatgtc tttcaagggc attggaagga ctgcaggaaa
     8941 tgaaggaacg tgagggacag gccatgcaaa gtgacattca gcaacgactt tcctcgtgtg
     9001 gaaacatcgt aaattcaatt gaaaaactct cgcaagcaga accggagcga ttcagggatc
     9061 gcttacagga acgtttttct caactgactg aaggtgttga aataaatcct gagcgtctgg
     9121 aacaggaaat tgctctgctt gcggatcgtc ttgatattag cgaagaagtt gtacgtttcc
     9181 ggactcatct ggaacacatg gatgatatat tgtctcaaca tgaagttgga aaaaaagcag
     9241 agtttctgat gcaggaacta aatcgtgagg tcaacacgat ggcttccaaa agcaatcatg
     9301 cgaaaattag tcaagcttcg gttgaaatca aaagcgagct ggaaaaagtc agggaacaac
     9361 tgcagaatat cgaatagagt cttgatgaaa ataactttgg ccggaagccc gggttcagga
     9421 aaatcaaccc tgcgtcgcat gctggcggag cattatggtt taaaaactaa agggaccggt
     9481 gattttatgc gtgaattgtc caggaaacat ggctacagcg acatcacgca atttctcgtt
     9541 gagtatgtgt cagagcatcc tgaagtggat catcaggttg atgaggagca gaggctctat
     9601 ggagagcaaa atgaggattt tgttttggat gcgcatttgg gattttactt tgttccggac
     9661 tcgatccgaa tttgtctaat ttgtgacctg gaagaagcgg caaggcggat actggaggac
     9721 actgaacgta ctactgaaga agctaaaact atcagagaaa gtattgatac cagccaaaaa
     9781 cgcagggata caatgcgaag aaatttttta agtctttata aagttgatat tgatgacaga
     9841 tcaaactttg atttgttgat ggatacgtcc agtctttcgt cggcagaagt ttttgagagg
     9901 gtttcaactt ttattgatga acagacttcc aatatataat tcgtttttcg aaagcatgac
     9961 ataatttatg gtttgtctct gatcattttt attgtgatac ttcacagaac tccccttacg
    10021 caccagtagc tcaactggat agagcaactg acttctaatc agtaggtt
//
LOCUS       DTVD01000081            2495 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_17699_length_2495_cov_315.834016, whole genome
            shotgun sequence.
ACCESSION   DTVD01000081 DTVD01000000
VERSION     DTVD01000081.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_2495_cov_315.834016"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="EYO68_02925"
     CDS             complement(<1..297)
                     /locus_tag="EYO68_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06790.1"
                     /translation="MLLRQIFAHAYPFENSDSGVDFSSPDTKIQAVNSGRLSFVFLAE
                     QNGINAFLKIVKPGFVRDNIAFSVLCTEECRLHSFIPTFQTYRGKNSELHQTVPE"
     gene            complement(352..>2495)
                     /locus_tag="EYO68_02930"
     CDS             complement(352..>2495)
                     /locus_tag="EYO68_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIC06791.1"
                     /translation="YFIVSFTPQALELVETNVNSPSWAIPRQLKLALPEDGKFPVSGK
                     KSLAAGPFYDPAQDKMIGGIINTFSTIAFAETTFGLLRSEKQVGSVSELNKRSVANRA
                     AVENWVSAHPLFEMGVQDTTRRGAAVTLLKVNDADVSDSDQHVKIIAKTKQLLGFEGL
                     THPNGDYERGLDVARYVNTFPGTPGDFRLWIGGSRPVSEITAVFDNLEYAYHRAKIVV
                     LEEELAKAGVTFEVSAAADSKMRKDDQNRAYKVLIADLVGLKFDSNGNPDFSEVQGHI
                     EEKGGVFHVGSVADHADLETGKIHFFYQPDLSRAEEIIPQTDQGQYDALIAAATFFPK
                     ESVFHSGGVRIGAGTGNMGSASWGGGNGAGGVAPLMNTPSFNSRATAHMTFKALLKTT
                     PDLDVGTLHQLVVEKNFDTGKQLKDFPTEKIEGKRIGIVGIGNIGREVAKIAQAFRME
                     VVVHARPRHQKWIESEGFIYAPSIEDVAKGADFISFHTGLGAPNPESGKFENEGMIGE
                     SVLNGLNDGAVLINYDRGEVVDAQALDKALASGKIRYAAIDADIFKNPSTGEITGPMA
                     PYLDLEKKYSGKLELLPHAAADTEHVSRVEGAKQAVDQIFSVIHFKTTINLKGDLPEG
                     YSDGGATTVSGVGKVTPKRLSETVTEDEFLSKMRQTTEEITAIWGALASTPNPERRAE
                     LIERYGSQLILASNSYASLIEGAGLKGPYSE"
BASE COUNT          623 a          631 c          561 g          680 t
ORIGIN      
        1 ttccggtaca gtctggtgta attctgaatt tttaccgcga tatgtctgaa aagtggggat
       61 gaaactatgc agtcggcact cttctgtaca gagcacagaa aacgcaatat tgtcgcggac
      121 aaatcctggt ttgactattt taaggaaggc attaattccg ttctgttccg ccagaaaaac
      181 aaaactcagc cgccctgaat taacggcttg aattttggtg tccggagaag aaaaatcgac
      241 cccggaatcg gaattttcaa acggatatgc atgggcaaaa atctgccgta agagcatata
      301 ttggtcgtct tcaagcaggt gatggcgtgc gattttgatc tcctaattat tttattccga
      361 atacggccct ttcagtcctg caccttcaat caaagacgca taggaattgg aggccaaaat
      421 caattgcgaa ccataacgct ctatcagttc cgctcgtcgt tccggattcg gagttgatgc
      481 caacgcaccc caaattgcag tgatctcttc ggtggtctga cgcatctttg agagaaactc
      541 atcttcggta accgtctcac tcaatcgttt cggtgtgact ttgccgactc cggaaactgt
      601 ggttgcgcca ccatcagagt atccttcggg caaatctcct ttgaggttga tcgtggtttt
      661 gaaatgaatc accgagaaaa tttgatccac tgcctgtttg gcaccttcaa cacgggaaac
      721 gtgttctgta tcggctgccg catggggtaa aagttccagt ttcccggaat actttttttc
      781 taaatcaagg taaggagcca ttggtccggt aatttcgccg gtggacgggt ttttaaatat
      841 gtctgcgtca attgccgcat agcggatttt tccgctggcc agggctttat ccaacgcctg
      901 cgcatcaaca acctcaccgc ggtcgtagtt gatcagcacg gctccatcgt tcaaaccatt
      961 cagcacactt tcgccaatca tgccttcatt ctcaaattta ccgctttccg gattcggggc
     1021 acccaatccg gtatggaagc tgataaaatc cgcacctttg gctacgtctt caatggaagg
     1081 agcgtaaatg aaaccttctg actcaatcca tttttgatga cggggccttg cgtgaacgac
     1141 aacttccatc ctgaacgcct gagctatttt tgcgacttca cggccaatgt tgccgatgcc
     1201 tacgatgcca atacgcttgc cttcaatttt ttccgtggga aaatctttga gttgctttcc
     1261 cgtatcaaaa ttcttctcta ccacgagttg atgcaaagtg cccacatcca aatccggagt
     1321 tgttttcagc aaggctttga aggtcatgtg cgcggtggca cgactgttaa aactgggggt
     1381 attcatcagc ggcgcaacac cgccggctcc gtttccgccg ccccaagagg cactgcccat
     1441 atttccggtt ccggctccga ttcggactcc tccggaatga aacacagatt ctttaggaaa
     1501 aaaagtcgcc gcggcaatga gtgcgtcata ttgcccttga tcggtttggg gaataatttc
     1561 ttcggcacgg ctcaaatccg gctgatagaa aaaatggatt tttccggttt ccaaatcggc
     1621 gtgatctgca acagagccga catgaaagac accgcctttt tcttcaatgt gcccctgcac
     1681 ttcactgaaa tcagggtttc catttgagtc aaatttcaaa ccaaccaaat ccgcaatgag
     1741 cactttataa gctcgattct gatcgtcttt tctcattttg gaatcggcgg cggcggaaac
     1801 ctcaaacgtc actccagctt tcgccagttc ttcttccaga accacaattt ttgcccggtg
     1861 gtaggcatat tcaagattat caaacacagc agtgatttct gaaaccgggc gtgatcctcc
     1921 gatccacaga cggaaatctc cgggcgttcc cggaaaggta ttgacgtacc gcgccacatc
     1981 caatccgcgc tcatagtcac cattcggatg ggtcaaaccc tcaaagccca aaagttgctt
     2041 ggttttggcg atgattttca catgttgatc ggagtcacta acatcagcat cattgacttt
     2101 tagcaatgta accgcagccc cacgccgggt tgtatcctga acacccattt caaaaagcgg
     2161 atgtgctgag acccaattct ctactgcggc tcgatttgct acagaacgtt tgttgagttc
     2221 actcactgac ccgacctgtt tttccgagcg gagcagacca aaagtcgttt ccgcaaaagc
     2281 gatagtactg aaagtgttaa taattccgcc aatcattttg tcttgtgcag ggtcgtaaaa
     2341 tggtccggcg gctaaacttt ttttaccgga aaccggaaac tttccatctt ccgggagagc
     2401 aagtttgagt tgacgcggaa tggcccacga tggactgttt acgtttgttt cgaccaattc
     2461 caaggcttgt ggagtaaagg acacaataaa atatc
//
LOCUS       DTVD01000082            9794 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1800_length_9794_cov_215.092925, whole genome
            shotgun sequence.
ACCESSION   DTVD01000082 DTVD01000000
VERSION     DTVD01000082.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1800_length_9794_cov_215.092925"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            576..1070
                     /gene="aprB"
                     /locus_tag="EYO68_02935"
     CDS             576..1070
                     /gene="aprB"
                     /locus_tag="EYO68_02935"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HIC06792.1"
                     /translation="MPSYVITEKCDSCAGQDKTACMYICPHDLMALTEQVDPITKKVI
                     VNPETNKPVMKAWNQEPVQCWECYSCVKICPQQAISIRCYADFAPLGGEVIPMLGSDS
                     IMWTIKFRNGNIKRFKFPVRTVDAGAVDIFSGVGEAQADVSRIKEPGFFTHHAIGVGL
                     PTPA"
     gene            1104..>2892
                     /gene="aprA"
                     /locus_tag="EYO68_02940"
     CDS             1104..>2892
                     /gene="aprA"
                     /locus_tag="EYO68_02940"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HIC06793.1"
                     /translation="MEPKVNNWEFCEKPEIVVHETDLLLIGGGMACCGAAYEADRWAT
                     PEGLRVTMVDKAATDRSGAVAMGLSAINTYLGDNSPEDYVRYVRNDLMGITREDLTFD
                     LGRVVDDSVYLFEDWGLPIWKKDKEGKSVDGARAQREEMPSLRDGGTPVRSGRWQIMI
                     NGESYKVIVAEAAKLALENNRKATGVDQNHFERVFIVKLLLDKDDPKKVAGAVGFSVR
                     EHKVYIFKCKTALLGTGGCVNIFKTRSTAEGQGRAWFPVWNSGSNYAMAAESGAEMTM
                     MENRFVPARFKDGYGPVGAWFLLFKAKATNALGEDYCATNLEESRKLYGKYVDSLGTC
                     MRNHMMMIDMKAGKGPIKIHTDVALQTLSETMSKKEIKHLEAEAWEDFLDMTIAQAGV
                     WAANNMEPEKVPSELMPSEPYLLGSHAGCAGLWTSGPEDFGPDEYSWGYNRMTTINGL
                     FTAGDGVGASGHKFSSGSHAEGRIAGKSMTSYFLDTKGEDVGYGEDHEELAKEIYMPM
                     ETFGKFSGYTTDPDINPHYIRPAMMQQRLQKVMDEYVGGVGTWYMTSKTMLEEGFKYL
                     VMIKEDALHMAAENLHELLRAWENFHRILA"
     assembly_gap    2893..2992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2993..4222
                     /locus_tag="EYO68_02945"
     CDS             <2993..4222
                     /locus_tag="EYO68_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIC06794.1"
                     /translation="VIGGGISGITTALEASEVGRDVILVEKSPTLGGRVSGFHQYFPK
                     LCPPTCGLEINYRRLRANPHVQVYTNSEVLSVSGEAGNFKIKVRKNPTYVNEKCVACD
                     HCTAVCPSDRSNDFNAGMDKTKAIFLPNKMAYPPRYVIDRPACEPGCSKCVAACKYGA
                     IDLDMKTQEIELEVASVVVATGWKPYDASKLTNMGFSSVPNVINNIMMERLASVSGPT
                     KGKILRPSDQKPVESVVFVQCAGSRDENHLPYCSSICCLGSLKHAKYVLEQNPEAKVH
                     IFYIDIRTPGKYEDFVVKVQAEPNVNMIKGKVAKIIAGKEGGVLVEAEDILKLGKVHL
                     EVDMVVLATGMEPSLKGQAQVAGIKLDEYGFVPMELQQQGIFSAGVAKWPADVNSSIQ
                     DSTGAALKAIQTTMGVN"
     gene            4222..6378
                     /locus_tag="EYO68_02950"
     CDS             4222..6378
                     /locus_tag="EYO68_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIC06795.1"
                     /translation="MESKIGVYICSGCDIDQALDVDELVKVAGKECKAPVSKTHPFLC
                     SEEGVQLMKEDQKNEGVNRFMIAACSQRYHEATFDMGDDSLVVRAPIREYVAWTQKTK
                     DENGEFDEDTQLAGEEYIQMYYAKIKKHGIPEAFEQDTSKNLLVIGGGVSGMTAAMEA
                     ANAGYSVNLVEKEDHLGGFCLDEYKLIPAQAPFRDPEMNSVSEAVSAVASNNLITVHA
                     SSFVVSISGQPGEFQVKLNSEGEFKEFKSGAIIMATGSHPYDAEKLTELGIQYENVIS
                     SAEFEQMAKSGNIQRKDGKPALNIGFIQCAGSRTPEHLPYCSGTCCMDSLKQAAYIRE
                     QNPDAKAHIIYRDMRTPGLYEDFYRTRQDDPGVFLTQGDVVGVNETESKNIAIDVDNT
                     LFGEPVTMEMDLVVLAVGQVPSTMKGESALNLQYRQGPDLPELKYGYPDSHFICFPYE
                     TRRTGIYAVGSVRQPMDINDAKLDATGAALKAIQAMELTDQGKTVHPRVGDLTFPEFA
                     LSRCTSCKRCTEECPFGVLEEDDKGMPLPNPARCRSCGICMGSCPVQIISFKDSSPNI
                     FKEMMTSYEMPDEFDEKPRVLIFVCENDALPVFDMAALERLKINTNVRIMPMRCLGGT
                     NLIYVTDALSYGYDGIMFMGCAFGDDYQCHFVNGSELANMRLGKVQETIGRLNLEPER
                     VRQYAISMNDYEKLPKIIDDFVEELEDFGPNPFKGM"
     gene            6454..8307
                     /locus_tag="EYO68_02955"
                     /pseudo
     CDS             6454..8307
                     /locus_tag="EYO68_02955"
                     /inference="COORDINATES: protein motif:HMM:PF13183.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307642.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    7608..7707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8380..8865)
                     /locus_tag="EYO68_02960"
     CDS             complement(8380..8865)
                     /locus_tag="EYO68_02960"
                     /inference="COORDINATES: protein motif:HMM:PF00515.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06796.1"
                     /translation="MPQRLKTLESTKELLLSAKEALVKKNLVKSIELFSKVLEHEPDN
                     LVALISRGTAYFSNKEFQPALHDFTKGIDLKPDSAKLYCSRGNAWLGLKQNESALNDL
                     NKAVELDPHYPTAYFSRSEVLERMDEKEQADLDRETAERLQKQISQAYLVSQGIEFQE
                     I"
     gene            8880..9704
                     /locus_tag="EYO68_02965"
     CDS             8880..9704
                     /locus_tag="EYO68_02965"
                     /inference="COORDINATES: protein motif:HMM:PF03692.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIC06797.1"
                     /translation="MDTSTQITNSDNHSEENTENFNLKPIPPEGIFSFSCHPGVSCFN
                     RCCHEIDVILTPVDILRMKTELKMRSDEFLTKFTDFQNLKETGIPMVKLRMRENSNGA
                     CIFLAGKQGCSIYHNRPLVCRSYPLGVASLDPRQADTLTEGQSTEARFMIQEDMCKGH
                     LEPKTWTLKEWMKDQGTLDLEKENKPWLEIVAKLKAMKIGEKQQREISLFIMASYDLD
                     TFRQFVFESTFLERFDVDEKTVESIRSDEKDLFTFGMEWLQFALFNEGTIRPKVNK"
BASE COUNT         2728 a         1992 c         2333 g         2541 t
ORIGIN      
        1 ccggaacatt ttcaaaccag ctctcacggc cttttttcct ttgtccccct ggtctgaaaa
       61 ttcttacaga atgtataatt aaggatctgc cgagttagac gataaccgta acccggaaaa
      121 tcaaaacata ggggaaatgt ttcaaatctt gtactcaaac tggacggtga tctggatccc
      181 ggatcacgtc cgggatgaca aaagaaaaac tttttcagtg agtcttatta aacgtgtagt
      241 taaaaatgtt tgtttcacag tacaaatgta aataaattca atagttgata aaatcgcaaa
      301 aagtttaatt tttctaatga gtaattaaga gtaaattttg aatttagtac ttgatatttt
      361 tttcaaaaac actagtctca tccccaatca atagctaaac ctaatgcagg gttcgaacat
      421 tatattaact tatctgtata tttagacata atatattgtt tccgcaataa gtatgataga
      481 gattgaattg tttgcaagtt ttgttgtttt aagactatta tagaagggag aaagtgcagt
      541 acaccaaaaa aattttaaat caataaggag ggctgatgcc aagttatgta atcacagaga
      601 aatgtgacag ttgtgccggt caggataaga ctgcctgcat gtatatttgt ccacacgatt
      661 tgatggcttt gacagagcag gtggatccga ttaccaaaaa agtcattgta aatccggaga
      721 ccaacaaacc tgtaatgaaa gcctggaatc aggagccggt gcagtgctgg gaatgttaca
      781 gttgtgtaaa gatctgcccg cagcaagcaa tcagcatccg ctgttacgca gattttgcgc
      841 ctttgggtgg agaagtcatt ccgatgctcg gttcggacag tattatgtgg accatcaagt
      901 ttcgtaacgg gaacatcaaa cgattcaagt ttcccgttcg taccgtcgac gcaggagcgg
      961 tggatatttt ttctggagtt ggagaagccc aagcggacgt ttcacgcatc aaagaacctg
     1021 gatttttcac ccatcacgca atcggcgttg ggttaccaac tcccgcataa aagaatgatc
     1081 actaaagaat aaagagtcga aatatggaac caaaagtaaa taattgggaa ttttgtgaaa
     1141 agccggaaat tgtggtacac gaaactgatc ttctcctgat cggcggagga atggcctgtt
     1201 gtggagccgc ttatgaagca gaccgctggg caactccgga agggcttcga gttacaatgg
     1261 tagacaaggc tgcaaccgac cgcagtggtg cggtggccat gggactttcc gcaatcaaca
     1321 cataccttgg cgacaacagc cctgaagact atgtgcgtta tgtgcgtaat gacttgatgg
     1381 gaattacccg tgaagatttg acgtttgacc tgggacgtgt tgtagatgat tctgtttatc
     1441 tttttgaaga ttggggactt ccaatctgga agaaagataa ggaagggaaa tccgttgatg
     1501 gtgcccgtgc gcagagagag gaaatgccgt ccttacgtga cggtggtact cctgtacgtt
     1561 ccggacgctg gcaaatcatg atcaacggtg aatcctacaa agtgattgtt gctgaagcgg
     1621 caaagctggc tttggaaaac aaccgtaaag caaccggtgt tgaccaaaat cattttgaac
     1681 gtgtatttat tgtcaaactg ttgcttgata aggacgatcc gaaaaaagtt gccggagccg
     1741 ttggctttag tgtgagggaa cacaaggttt atattttcaa atgtaaaact gctctgctgg
     1801 gaaccggagg ttgtgtaaac atcttcaaaa cccgttcaac agcagagggc caaggacgcg
     1861 cgtggtttcc agtttggaat tcaggctcaa actatgccat ggccgcagag tctggagcag
     1921 aaatgacaat gatggaaaac cgttttgttc ccgcgcgttt caaggacggt tatggtccgg
     1981 tcggcgcatg gttcctgctc ttcaaggcga aagcaaccaa tgcgcttggt gaagattact
     2041 gtgcgacaaa ccttgaagaa agccgcaagc tttacggtaa gtatgtggat tccctgggaa
     2101 cctgcatgcg taaccacatg atgatgattg acatgaaggc cggtaaaggc ccaatcaaga
     2161 ttcatactga tgttgctctc caaacactat cagagactat gagcaaaaag gaaatcaaac
     2221 acctcgaagc agaagcctgg gaagattttc ttgatatgac cattgcgcag gccggagtct
     2281 gggcagcaaa caatatggaa ccggaaaaag ttccgtcaga attgatgcct tctgagcctt
     2341 acttgctggg ttctcacgca ggttgcgcag gattgtggac cagcggtcct gaagattttg
     2401 gaccggacga gtattcctgg ggctataatc ggatgaccac aatcaacggt ctctttactg
     2461 ccggagatgg agttggggct tccggacaca agttttcctc ggggtcacat gccgagggac
     2521 ggattgccgg taagtccatg acctcctact ttcttgacac aaaaggagag gatgtcggtt
     2581 atggcgaaga tcatgaagag ttagccaagg aaatctacat gccgatggaa acattcggaa
     2641 agttcagcgg ttacaccaca gatcccgaca tcaatccgca ctacattcgc ccggcaatga
     2701 tgcagcagcg tttgcagaaa gtgatggacg aatatgtcgg tggtgtcgga acctggtata
     2761 tgaccagcaa aacaatgttg gaggaaggct tcaaatattt ggtaatgatt aaagaggatg
     2821 ccctgcacat ggctgcagaa aatctccacg aattgttgag agcctgggaa aacttccacc
     2881 ggattcttgc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgatcgg
     3001 tggtggaatc agcggcatca caacggccct cgaagcctct gaggttggtc gagatgtgat
     3061 cctcgtcgaa aaatctccta cactcggcgg gcgcgtttcc ggattccatc agtattttcc
     3121 caaactttgt cctccaacct gcggacttga aataaattac agacgtttgc gcgccaatcc
     3181 gcatgttcaa gtttacacca actctgaagt cctttcggtt tccggagaag caggtaattt
     3241 caaaatcaaa gtccggaaaa atccaactta tgtaaatgaa aagtgcgtgg cctgtgacca
     3301 ctgtactgcg gtttgtccat ctgatagaag caacgatttc aatgcaggaa tggacaaaac
     3361 caaagcgatt ttcctgccca acaaaatggc ctatcctccc agatacgtga ttgaccgtcc
     3421 ggcctgtgaa ccgggatgca gcaaatgtgt ggcagcctgc aagtacggtg cgatcgatct
     3481 tgatatgaag acgcaggaaa tagaactgga agtcgcctcg gtcgtggtag caaccggctg
     3541 gaaaccttac gatgcctcga aactcaccaa catgggtttt tccagcgttc caaatgtgat
     3601 taacaatatc atgatggaac gacttgcctc cgtcagcggc cccaccaaag gtaaaatttt
     3661 acgcccatca gaccagaagc cggtagaaag cgtagtcttt gtgcagtgcg ccggttccag
     3721 agacgaaaac cacctgcctt attgttcctc gatttgctgt cttggctcgc tgaaacacgc
     3781 aaaatatgtc ctggagcaaa atccggaggc taaggttcat attttttaca tcgacatccg
     3841 tactcccgga aagtacgaag attttgtggt gaaagtacag gcagaaccta atgtgaatat
     3901 gatcaaaggc aaagtcgcca agatcattgc cggcaaagaa ggaggagtcc tggttgaggc
     3961 agaagatatt ctcaagttgg gcaaagtcca tcttgaagtg gacatggtcg tgctggctac
     4021 cggaatggag ccaagcctga aaggtcaagc acaagtagca ggaattaaat tagatgaata
     4081 cggatttgtt ccaatggagc tacaacaaca aggaatcttt tcagccggag tggccaaatg
     4141 gccggccgat gtcaacagtt ctattcagga ttcgaccgga gctgcattga aagcgattca
     4201 aaccactatg ggggtgaatt aatggagagc aagattggcg tttatatttg ttccggatgc
     4261 gacattgatc aggctcttga tgtcgatgaa ctcgtaaaag ttgccggtaa agaatgcaag
     4321 gctccggtgt ccaaaacaca tccgttcctc tgtagcgaag aaggtgtgca gctgatgaag
     4381 gaggatcaga aaaacgaagg cgtgaaccgt tttatgattg ccgcctgttc gcagcgttac
     4441 catgaagcaa cctttgatat gggcgacgac agcctcgtgg tccgtgcgcc gattcgtgaa
     4501 tatgtggcat ggacacaaaa aacaaaagat gaaaacggcg agttcgatga agacacgcag
     4561 ttggccggag aagaatatat ccaaatgtac tatgcaaaaa ttaaaaagca tggaattccg
     4621 gaagcattcg aacaggacac ctctaaaaat ctgttggtaa tcggcggagg cgtgagcgga
     4681 atgactgctg caatggaagc cgcaaatgca ggatattccg tgaatttggt ggaaaaagaa
     4741 gatcatttgg gcggattctg tttggacgaa tacaagctca ttccggcaca ggcaccgttc
     4801 cgcgatccgg aaatgaactc cgtttccgaa gctgtttccg cagttgcaag taacaattta
     4861 atcaccgttc atgcttcatc ttttgtggtt tccatttccg gtcagccagg agaatttcag
     4921 gtgaaactca attctgaggg cgagtttaaa gaattcaaaa gcggcgcgat catcatggcc
     4981 accggctctc atccgtatga tgccgaaaaa ctcactgaat tggggattca gtatgaaaac
     5041 gttatcagca gtgccgaatt tgagcaaatg gccaaatccg gaaatattca gcgcaaagat
     5101 ggaaaaccgg ccttgaacat cggattcatt caatgcgcgg gttcgagaac tccggagcat
     5161 ttgccttatt gttccggaac ctgctgcatg gattcgctta aacaggcggc gtatatccgt
     5221 gagcaaaatc cggatgcaaa ggcgcacatc atttatcggg atatgcgcac acccggactt
     5281 tatgaagact tttaccgcac acgtcaggat gatccgggtg ttttcctgac tcagggcgac
     5341 gtggtcggag ttaatgaaac cgagagcaaa aacatcgcga ttgatgtgga caatacgttg
     5401 tttggcgaac ccgttacaat ggaaatggat ttggttgttt tggcggttgg tcaagtgcct
     5461 tcgacgatga aaggcgagtc cgctttgaat ctgcagtacc gccagggacc ggaccttccg
     5521 gaactcaaat acggctatcc ggattctcat ttcatctgct ttccttatga aacccgccga
     5581 accggaattt acgcggttgg ttccgtgcgt cagccgatgg acattaacga cgcaaaactt
     5641 gatgctaccg gagctgctct aaaagccatt caggcgatgg aattgaccga tcaaggcaaa
     5701 accgttcatc cgcgtgtggg agatttgact tttccggaat tcgcccttag tcgctgtact
     5761 tcctgcaaac gctgtacgga agagtgcccg tttggtgtgc ttgaagaaga tgacaaagga
     5821 atgccactcc cgaatccggc gcgttgccgg agctgtggga tttgcatggg ttcctgtccg
     5881 gtacagatta tttcgtttaa ggattccagt ccgaacattt tcaaagaaat gatgacgagt
     5941 tatgaaatgc cggatgaatt tgacgaaaag ccaagagttt tgatttttgt ctgtgagaat
     6001 gacgcacttc cggtttttga catggcggca ctggagcgtt tgaaaatcaa tactaatgtc
     6061 cggattatgc cgatgcgttg tttgggcggc acaaatttga tttatgttac agatgcattg
     6121 tcgtatggat acgacggaat catgttcatg ggctgtgctt ttggtgatga ttatcaatgt
     6181 cactttgtca atggcagtga gttggccaat atgcgtttgg gcaaggtgca ggagaccatc
     6241 gggcgtttga atttggagcc ggaacgggtt cggcagtatg caatcagcat gaacgattac
     6301 gaaaaactcc caaagattat tgacgatttt gtggaagaac tggaagattt cggtccaaat
     6361 ccgtttaaag ggatgtgatg gtcagttttt tgattgaaca cgtgtcctca gtaaattccg
     6421 gctggataga acaaccaaaa aaatgagcat gaaatgacag gacgagttgc cgtcaataca
     6481 gatttaaatt ttgtgcaggg actgatgctg catggagctg cggatctgaa aaaatgctac
     6541 cagtgctcca cctgtacggt agcctgtcct ttgactccga atgacgagcc gtttccacgt
     6601 aaagaaatgc tgtgggcaca gtggggcatg aaggataagc tgttcagaga catggacttg
     6661 tggctgtgtc acaactgcaa cgactgcacc gatcaatgcc ccagaggagc aaaaccggga
     6721 gaggtgatgt cggcgctccg caatcaaacc atcgaacact attcctaccc gcaggccatt
     6781 tctaaagcag caaaatcggc aagaggaaac gtcgtattgt ttttgattcc aatggtcctg
     6841 attgcccttg ctattgcaat gaaaaacatc gaaagcggct ttgcgttttt aaccgcaaaa
     6901 ccaattgttt acgcaaacat gctgacggtg aattttattg atatcctgtt tttgccgcca
     6961 gttttgtttg ctgtatttaa ttcctataag gcaatcagca acttcattgg cggactcaaa
     7021 gaacagtacc ctcccacaga cagaggagaa aatttactgc ctgccgtaaa aggcacaatc
     7081 caggatgtac tctcacacat tgaattcaaa aagtgcggca ctaacaaaag ccgcaatatt
     7141 tcacacagac tgatgatgta cggcttcatt ggacttttca taacgacaaa tgccgtgttt
     7201 gttttgcact ggctcaacga atttggtttt gatgttcagg tgacaccgct tccatttttt
     7261 catccggtta aaatcctggg gaacgtgagc gcctttgcgg cctttgccgg aatttatctg
     7321 attgtacggg accgtgtgaa aaattctggg acaagcattt tgtcgttgtt tgactggatg
     7381 ttcattggaa acatgttctt cacggttgta accggaattt tgtgccaggt ttttcgtgta
     7441 ggtgatcatg caaatctcgc atttttaacc tactatatcc atttagttat ggtcttttat
     7501 ttgctggcct atgcacctca gactaaattt ggtcatatcc tttatagacc tgcggcgatg
     7561 atttattccc gttattccgg acgcaacata gatattttca gaaacttnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnaca gtcattgctt ttcaaactcg taacccgatg
     7741 caccgtgctc atgaagagct ttgccggatg gcacaacatg aattgaatgc ggacggaatt
     7801 ttagtacaca tgctgctcgg taagttgaaa aaaggtgaca tcccggcaga cgtgcgcgat
     7861 gcctcaatcc gcaaaatggt tgaactgtac tttccggaaa atacagtgat ggttagcggt
     7921 tatggttttg atatgcttta tgcaggtcca agagaagcag tcctgcatgc ggtttttcga
     7981 caaaatgccg gatgcacgca tttgattgtt ggacgggatc atgccggagt tggggattac
     8041 tatggggcat ttgacgcaca aaccattttt gaaaacattc cggaaggtgc tttacaaatc
     8101 cggattttcc ggggagacca caccgtttgg tgtcataaat gtgaaaaggt gatgatgatg
     8161 cgtgattgcc cgcatgacaa agaggactac ctactgctct ccggaaccag ggttcgtgaa
     8221 atgctttcaa aaggagaaga acttccacct gagttttcac gtccggaagt tgcaacaatt
     8281 ttgtctgaat actaccagac tttgtaagtt atttttatga atgatctgaa acaggttctg
     8341 cgcagtcgca aaaatactag tcagggagag gaatcctgct taaatttcct gaaactcaat
     8401 tccttgagat actaaataag cctgggaaat ttgtttttgg agtcgttctg ctgtttctct
     8461 gtctaagtcg gcctgttctt tttcatccat gcgttccaaa acttcgcttc tgctgaaata
     8521 tgcagtagga tagtgcggat ccagttctac agctttattg agatcgttta gagcagattc
     8581 attttgttta agacccagcc aggcattgcc ccggctgcaa tataacttgg cactgtctgg
     8641 cttaaggtca atccctttgg tgaaatcatg cagggccggc tgaaattcct tatttgagaa
     8701 ataagcagtc cctctactga ttaaagctac caggttatca ggttcgtgct ccaaaacttt
     8761 tgaaaagagc tctatacttt ttaccaaatt ttttttaacc aatgcttcct ttgcggagag
     8821 caaaagttct ttcgtagatt ccaatgtttt caacctctga ggaataagta attatttaaa
     8881 tggacacttc gacacaaatt acaaactctg ataaccattc tgaggaaaat acagaaaatt
     8941 tcaatctaaa gccgatccct ccggaaggaa ttttctcttt ttcctgtcat cccggtgttt
     9001 catgcttcaa tcgctgctgt cacgaaattg atgtcatttt aactccagtt gatattttga
     9061 gaatgaaaac tgagttgaaa atgcgctcag atgaattcct gacaaaattt accgattttc
     9121 aaaatttgaa agaaactggg attccgatgg tcaaacttag gatgcgtgaa aattcaaatg
     9181 gagcctgtat ttttttggca ggtaaacaag ggtgttccat ctaccacaac cgaccgctgg
     9241 tttgcagaag ttaccctctt ggagtagctt ctctcgatcc aagacaagca gatactctga
     9301 ccgaagggca gtctacggaa gcacgcttca tgatccagga agatatgtgc aagggacacc
     9361 tggaaccgaa aacctggacg ctgaaagaat ggatgaaaga tcaaggcacc cttgacctgg
     9421 aaaaagaaaa caagccctgg ttggaaatag tagccaaatt aaaagccatg aaaataggag
     9481 aaaaacagca gcgggaaatt agtttgttta tcatggccag ttacgatctt gatactttca
     9541 ggcaatttgt ttttgaaagt actttcctgg aacgctttga tgtggatgaa aaaactgtag
     9601 aatcaattcg ttcagatgaa aaagatcttt ttacattcgg aatggaatgg cttcagtttg
     9661 ctttattcaa tgaggggacg attcggccaa aggttaacaa atgagcaatt tttttagcct
     9721 ctctggaatt gataaataga aaaaaacttg actattattg tcaaacaagg gaaacacagc
     9781 aatattttta tgat
//
LOCUS       DTVD01000083            2466 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_18030_length_2466_cov_264.428868, whole genome
            shotgun sequence.
ACCESSION   DTVD01000083 DTVD01000000
VERSION     DTVD01000083.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_2466_cov_264.428868"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="EYO68_02970"
     CDS             complement(<1..980)
                     /locus_tag="EYO68_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC06798.1"
                     /translation="MKKFIRAMAATAVMVMVGFVGQAMATELVVVSWGGAYTASQQKA
                     YHAPYMKLNPGITIINDDSAGSAVAKLRAMSQAGNVTWDLVDAVASDTMILCDEGLIE
                     VIDHDRDLAPAPDGTPASKDFGGFIVSECFVPQIVYSTTFGYRKDLVSRPMSSICDIF
                     DLKSFPGKRSLEKKPINNLEWALICDGVAPAKVYDVLGTSAGVDRALAKLDTIKSQVV
                     WWTAGAQTPQLLADGEVIVGSTYNGRLFALIEEQKQPIAMLWDWQVFDLDGWIVPKGT
                     KNKAEVMKFLRFATDTQRLADQAKFISYGPTRASSAPLVGKHATLGIEMAP"
     gene            complement(1125..2222)
                     /locus_tag="EYO68_02975"
     CDS             complement(1125..2222)
                     /locus_tag="EYO68_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06799.1"
                     /translation="MSSSEAYVSFQGVQKSYDGETLVVKDLNLDTQRGEFLTMLGPSG
                     SGKTTCLMMLAGFETVTHGEIILDGTSINNVPPHKRNIGMVFQNYALFPHMTVNENLA
                     FPLQVRKMSKSDIEDRVKNVLDMVQLGSFGSRRPMQLSGGQQQRVAVARSLVFEPKLV
                     LMDEPLGALDKQLREQMQYEIKHIHENLEVTVVYVTHDQSEALTMSNRIAVFDDGVIQ
                     QLAEPDLLYEAPENAFVAQFIGENNTLYGKVIGLDGDICTVEAENGDKLKALGVNIQG
                     VGFDTMISLRPERVEVNPESGQCSSTVTGKVEELIYLGDHIRSRMNVCGHDDFIVKIP
                     NSANHAQLSPGDTVQVGWKAEDARALDVLVS"
BASE COUNT          720 a          597 c          477 g          672 t
ORIGIN      
        1 ggagccattt cgattccaag agttgcgtgt tttcctacaa gaggtgcgga agacgcacga
       61 gtcgggccat atgaaatgaa tttggcctgg tcagcaagac gctgagtatc ggttgcaaaa
      121 cgcaaaaact tcatgacctc agctttgttt ttagtacctt taggaacaat ccaaccgtca
      181 aggtcaaaca cctgccagtc ccagagcatg gctattggtt gcttttgctc ctcgatcaaa
      241 gcaaataggc gaccgttgta agttgaacct acaataactt cgccgtcagc caataactgt
      301 ggagtttgcg ctccggcagt ccaccagact acttgacttt tgatcgtatc caatttcgcc
      361 aatgcacgat ctactcccgc acttgtaccc aagacatcat aaaccttagc cggagcaaca
      421 ccgtcacaga tcagtgccca ttccaagtta ttgattggct tcttctccaa agagcgttta
      481 ccgggaaaag acttaagatc gaagatatcg caaatactgg acatcggacg tgaaaccaag
      541 tctttacggt aaccaaaagt cgtcgaatac acaatctgag gaacaaaaca ttcggaaaca
      601 atgaaacctc caaaatcctt ggaagccgga gttccgtccg gagctggcgc caagtctctg
      661 tcgtgatcga taacctcaat aaggccttcg tcacaaagta tcattgtgtc tgatgcaact
      721 gcatcaacca agtcccaggt gacattacct gcttggctca tcgcgcgtaa ttttgcgacc
      781 gctgatccgg cagaatcatc attgatgata gtgatgccag gatttaactt catatacggg
      841 gcatggtaag ctttctgctg actggcagta taagcaccac cccacgatac aacaaccaac
      901 tcggttgcca ttgcctgacc gacaaagccg accattacca taacagcagt tgccgccata
      961 gctcgtatga attttttcat actatctcct tttttacaat taaaaaaccc acaacgggct
     1021 atttttcaga aatccctgaa ttgggacctc ctattaaaag atgctggcag cgtgatgctg
     1081 gcagtttgag taaatccaat ggccaactcc tttattccag catcttatga cacaagtaca
     1141 tccaatgcac gtgcatcttc tgctttccag ccaacttgaa ccgtatctcc gggactcaat
     1201 tgtgcatggt ttgcagagtt tggaatcttg acgataaaat cgtcatgacc acaaacgttc
     1261 atacgggagc gaatatgatc tcccagataa atcaattctt ctacttttcc tgtaacagta
     1321 cttgagcatt gcccactttc cggattaact tcaactcgtt caggacgaag tgaaatcatg
     1381 gtgtcaaaac ctactccctg tatgttgacg ccaagtgcct ttagtttatc cccattctct
     1441 gcctctaccg tacatatatc gccatcaaga ccgatgactt taccgtaaag ggtgttgttc
     1501 tccccaatga actgggccac aaaagcattt tccggtgctt cgtaaagcaa atccggttcg
     1561 gccagttgct gaattacgcc gtcatcaaaa accgcaatgc ggttggacat ggtcaaggct
     1621 tcactttgat cgtgagtcac ataaaccact gttacttcca gattttcgtg tatatgcttt
     1681 atttcatact gcatttgctc acgcagttgt ttgtcaagcg cgcccaacgg ttcatccatc
     1741 aaaacaagtt taggctcaaa tactaaagaa cgtgccactg caactctctg ctgttgacct
     1801 ccggaaagtt gcatcggacg gcgtgaaccg aaagaaccca actgcaccat atccagaaca
     1861 tttttaacac ggtcttcaat atcgcttttt gacatctttc ggacttgcag gggaaaagcc
     1921 agattttcat tgacagtcat gtgtgggaac aaagcatagt tctggaatac catgccgata
     1981 tttcttttgt gtgggggaac gttattgatg gaggtcccat cgagaattat ttcaccgtga
     2041 gtcactgttt caaatccagc taacatcatc agacaggtag tctttcctga acctgaagga
     2101 ccaagcatag ttagaaattc acctctttgc gtgtctaaat tcaaatcttt gacgacgagt
     2161 gtttcgccgt catagctttt ttgaacaccc tggaaactga cataagcttc gctagaactc
     2221 atctttcctt atatataaaa cttaaataaa tataatctta ggcagtaaag cattctacat
     2281 cttgaaacga aagtgtcaaa cattttcttt ataaatctac caaaaaagaa taaacaactt
     2341 acgaaacact aaaaaataga ataatagacc aaaaactcac tgcaaaaaat gaaaattatc
     2401 cttgcaatgt ctggtaagtt aagtcagttt caatttggag tcgttcacta aaacatgatt
     2461 gagagc
//
LOCUS       DTVD01000084            9651 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1851_length_9651_cov_154.034702, whole genome
            shotgun sequence.
ACCESSION   DTVD01000084 DTVD01000000
VERSION     DTVD01000084.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_9651_cov_154.034702"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            96..1247
                     /locus_tag="EYO68_02980"
     CDS             96..1247
                     /locus_tag="EYO68_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC06800.1"
                     /translation="MPLHFDSKEFGNRRNRLLELMAGSELDGMLIFRQESMFYLTGYD
                     TFGYVFFQCLFLGGDGKLILLTRVPDLRQAQNTSIVEDIRIWVDGPEVNPALQLKEIL
                     REYGCAGKRLGVEYDSYGLTAKNGKMLDSALDGFCELIDASGMVNRLRVVKSPEELVY
                     VRKAAELADDALDEAHRLVKDGCDEGELLAAMQGAVFRGGGDYPGNEFIIGSGENALL
                     CRYFSGRRKLDPEDQITLEWAGVFRHYHAAMMRTIPVGRVTEQQRKMHEVCLESMAAC
                     ESVLRPGKAIGEVFDAYARTCDNLGMRAHRLNATGYSMGTTFSPNWMDWPMFYHGNQV
                     IAEKNMVFFLHMILMDSDSGIAMCFGHTVLVTDSSCERLSCHPLDLVVR"
     gene            1348..1684
                     /locus_tag="EYO68_02985"
                     /pseudo
     CDS             1348..1684
                     /locus_tag="EYO68_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            1803..2168
                     /locus_tag="EYO68_02990"
     CDS             1803..2168
                     /locus_tag="EYO68_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06801.1"
                     /translation="MVDKSNLLRQMRKHAQTFSSAVSSPLVVDEANINRALVLIDVAA
                     RDSGLGILRKMSFKTPALLKNLRLEKNLDYLKTEIKKRIPEAISSEGPLTKEQQKKEY
                     EKFKRAEQKLRKLLDTKDC"
     gene            2623..3546
                     /locus_tag="EYO68_02995"
     CDS             2623..3546
                     /locus_tag="EYO68_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIC06802.1"
                     /translation="MSLRINNNIESMNAHRNLLMNDRALSKSLERLASGQKINRASDD
                     PAALVISEHMRAQISGMEQAIRNNEVAISLVQTAEGSMNEISNILVNLRQRAISAANV
                     GASDQDMIDANQAEIENALASIDRVVSTTQFGHYKLLDGTNAAKTETNEDGSISTQEG
                     LRFHVGPNAEHMASTSIRDMSTSQLGRVIVLEGEMPNKSNFMSLADIDVRNEQGAQDA
                     LAIIDQALTEVATVRGELGAFQKHTLESNLTSLQVAAENMTAAESTIRDTDMAQELAT
                     FTRNQIMTQSATAQLAQANAMPQHVLRLLNG"
     gene            3739..5205
                     /locus_tag="EYO68_03000"
     CDS             3739..5205
                     /locus_tag="EYO68_03000"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00669.18,HMM:PF00700.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIC06803.1"
                     /translation="MPLGMKHNIASINAINHLAEAQNELSDSLKRLSSGQRVNSGADD
                     PSGLILSEGLRAQVASAQQALQNSEFSLSLVQTAEGALVEVNNLLIEMRQLATTAANE
                     GATDYNALLALQFQIRKAIEGIDRVSRFTSFGNKTLLDGSHGATGMGGNEELVFLKAS
                     SKTVASPVSGYDVDITELPLRATLTADLDDDDASGLQITLEEEDGAIIRVSNPEGATA
                     VGFAKRLQIAVLEANMNLNIRFDADDEELTIEHREYGLTKGFTVTGTKDDVIVDNAFE
                     PELLLGRDIEGTINDEPADGDGLILTGEYNNEKTSGLSVAFLGDGTGNAGSVTVAQNS
                     LKFQAGASADEKIVIALNSTHSTVLGRGVDNTSGFENLSQISLKSTQEAIDAIRLVDE
                     ALDQLLSMRSQLGSVQKHTLETNISVLRSTVENLTAAESSIRDTDMALEMVNFTKNQI
                     ITEAAAAAVAQSNQTATRVLRLLFNNNPHGHWSFFRDH"
     gene            5220..6593
                     /gene="mnmE"
                     /locus_tag="EYO68_03005"
     CDS             5220..6593
                     /gene="mnmE"
                     /locus_tag="EYO68_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIC06804.1"
                     /translation="MRLDDTIAAIATPLQPSGLGVIRVSGEQAVSAVEALFKTNGKPL
                     KKTETHKLVHGWIKDDGKLIDKVLVVVMRAPHSYTTEDVVEIQCHGSPFVLRIILDLV
                     LRQGVRLAGAGEFTQRAFLHGRLDLTQVEAISDLIQASSEIGARLAAQQLRGKLFHAI
                     DAVKKQVVSIASLVEASIEFPEEDHEFTHQEDCLQRLDQASADLESLLSHADRGRQIR
                     DGFSVVLIGRPNVGKSSLLNTLLREQRAIVTTIPGTTRDSIEESVQIQGLAFRLTDTA
                     GIRKTEDLIESEGIRRTQNVREKADLVLLILDASEKLMDEDLSLISEVEKERTIVVLN
                     KKDLMDDTTPRWYEKISRLESVLISAKTGEGCDELESSLYKKATLGGLPQEDEIWITN
                     RRQQQAAKNALSALFSARKSLNQSSGEEFLAVDLRSCLNALGEIVGETTPDDLLGQIF
                     SDFCIGK"
     gene            6583..6999
                     /locus_tag="EYO68_03010"
     CDS             6583..6999
                     /locus_tag="EYO68_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06805.1"
                     /translation="MENKQMTDSELKLLLEKQELLLKKLLELSQRQFAESDAVALDEL
                     LKQKDSYFDELQKLDPLREKWHNKYNRPLGQEEQKLDDNIQDLLEKLLLSEQDFEKIV
                     GREKNAVSLQIAQISNQMQYRKDTTRQRPQIKNMTT"
     gene            7024..7950
                     /locus_tag="EYO68_03015"
     CDS             7024..7950
                     /locus_tag="EYO68_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06806.1"
                     /translation="MLFSAYQIESLLADFQQTDSPAGKSASETLSLSADAIQDARQYL
                     RPLKRSPLNWVTPKDIRGLVEDISSYDPVVDNVQQSRAGRFVVPAYVPEIPAPAFPLV
                     SDHTQMAPESETTEQESSESPVTEEDALMPEINTESSEVEDKVSVSVSEDISKENSLI
                     IETEKSSEESEESTNGESAVSTASLFEGIDDEEHDEEHELEEEGLQGDNSEENDVVFG
                     DGRIDQKSMSNFVAHYPDSTLKFLMRKNLDGRPLPVGYEEIYSQWENRGLSRGRLKKY
                     LFKLMEWKNFPDIPVHDVVNKIREHQYFLEIK"
     gene            7950..8462
                     /locus_tag="EYO68_03020"
     CDS             7950..8462
                     /locus_tag="EYO68_03020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIC06807.1"
                     /translation="MTAKRFRPNVAAVIMHPSNKKVLMFRRIAKRNEKSELLSDGGQL
                     RWNWQFPQGGIDPGESEEETLFRELEEEIGTNDVTIIRVAEKRVRYYYPLKMLHILHK
                     QPEWKPYRGQQQRWFLLQLNCDTEKINFSHQPIEFDAFQWISPRLGLKKVVPFKRKAY
                     RKGLRYLGIL"
     gene            8631..9398
                     /gene="yaaA"
                     /locus_tag="EYO68_03025"
     CDS             8631..9398
                     /gene="yaaA"
                     /locus_tag="EYO68_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIC06808.1"
                     /translation="MLMLLSPSKTLDYETPATTDSFSIPEYLEKSSELVKVLKQKSFL
                     DLMELMQVSQKIAELNVERFNQWKLPFSTENAKQAVLAFKGDVYTGLDAPALSENRLA
                     YTQSHLRILSGLYGLLRPLDLMQPYRLEMRLKLTTKKGENLYQFWGEKITDALNVLLA
                     KQDEPVLINLASNEYFKSVQKKNLDGRLITPEFKDWKNGKYKMISFFAKKARGLMVRY
                     AIDHNIQKAEVLQNFDYDGYHFNLELSQADKWVFSRG"
BASE COUNT         2982 a         1820 c         2217 g         2632 t
ORIGIN      
        1 ttgtttttgt gagttgaaaa aatcagaatg aaggtctgtc attttttcta tgtacctgaa
       61 tattgcgaaa ttttatccaa ttacagtgag gaaatatgcc actacatttt gacagcaaag
      121 aattcggaaa ccggcgcaat cgcttactgg agttgatggc cggtagtgaa ttggatggga
      181 tgcttatttt caggcaggaa agcatgtttt atctaactgg atatgacaca ttcggctatg
      241 tttttttcca atgtctattt ctgggaggag acggaaaact gattttgctg acccgcgtac
      301 cggatttgcg gcaggcacag aacacgtcca tcgtagaaga tatccggatc tgggtggatg
      361 gacccgaagt aaatccggct ttgcagctca aggaaattct gcgggaatat ggttgtgctg
      421 gaaaaagact tggagtggaa tacgactctt atggcttgac tgctaaaaat ggaaaaatgc
      481 tggattccgc tctggacgga ttttgtgaat tgatagatgc ctctggaatg gtgaatcgcc
      541 tgcgggtcgt gaaaagcccg gaagaattgg tctatgtccg gaaggctgca gaattggcag
      601 atgatgctct ggatgaagca caccgcttag tgaaggatgg atgtgatgag ggagaactac
      661 tggcggccat gcagggtgca gttttcagag gtggtggaga ttacccggga aacgaattta
      721 ttatcggttc cggtgaaaac gctttgcttt gccgatattt ttccggaaga cggaaactgg
      781 atccggaaga ccagattact ttggaatggg ccggagtgtt tcgccattat cacgctgcga
      841 tgatgcgcac aattcctgtc ggaagagtga cagaacagca gcgaaaaatg cacgaagttt
      901 gtcttgaatc aatggcggct tgtgaatctg ttttaaggcc gggaaaggcc atcggtgagg
      961 tttttgatgc ttacgccaga acttgcgata acttgggaat gagagcgcac cggctgaatg
     1021 caaccggata cagtatgggg acaacttttt cgccgaactg gatggactgg cctatgtttt
     1081 atcacggcaa tcaagtgatt gctgaaaaga atatggtgtt ttttcttcac atgattttga
     1141 tggacagtga ctccgggatt gccatgtgtt ttggacatac cgtcctggta acggattcca
     1201 gttgcgaacg cctttcgtgt caccctttgg atttggtggt gcgttaaccg atcctccttg
     1261 gtcactttcc ggtgagacag cacggatgag tattactgtc tcgccgggaa gtgccatgtt
     1321 tttattatag acaggagaaa agtggaaatg gagagaaagg ctttgctgaa cgacaagtcg
     1381 tattatgccg tgatttttac ttcaatacaa agtgaagtga acaaggatta tgaggaaatg
     1441 gctgaactga tgatctcatt agcaaaaaaa cagcctggtt tcctcggggt agactcagct
     1501 cgaagtaatg gtatcgggat aactgtgtct tattgggaga ccgaggagtc tatcaggaat
     1561 tggaagaatc atgaaaaaca cctggtagca caacataaag gccggtctgg ctggtacagt
     1621 gattatcatg tccggcattg cacaggtcga aagagaatat gagtttgata gcaggaaaaa
     1681 atgaaataaa aaaattttaa cgtttaacat caagccgatt ttaaagaata tcgacagatg
     1741 tctataaaat tagatgaatg ttcattgtgt aattccaaat tagcgaaagg taaaaatcga
     1801 aaatggtgga taaatcaaat ctcttacgtc agatgcgtaa acatgcgcaa acattttctt
     1861 ctgcagtatc atccccctta gtggttgatg aagctaatat aaatagagcc ttggttttga
     1921 ttgatgtagc tgcaagggat tccggattag ggatactccg taaaatgtca ttcaagactc
     1981 cagctctgtt aaaaaatctg cgtcttgaaa aaaatttgga ttacttgaag actgagatta
     2041 aaaaacgaat tccggaggcc atttcaagtg agggtcctct gacgaaagag cagcaaaaga
     2101 aagaatatga aaaatttaaa cgtgccgaac aaaaattgcg gaaattactt gatacaaagg
     2161 attgctgaaa acatacgtcg ttaattttta ttaatttctt ggcctgagat caagaataat
     2221 atttgatatt tttattaata aaaataatat tttattgaaa tagaataccg gaaaatcgat
     2281 tggattttcc ggctgcattt gtctcttcaa ttgtagtatg gagaggactg tctggaaaca
     2341 aagcttgagg cccagaatac gaggctcaag actatgaatt cagctggcac agagtttcga
     2401 aataccaccg gacttttgtg tagtggtgtt ttaaaacctt cacatgccgt gcatgttgaa
     2461 ggctgagggg gtagggcttt gctttcatgg tgaaagctga ccctgtttga atgagtgcgg
     2521 attgaatccg gacttgtttg cccaaagtat tctattggaa gggaatacat ttattccatg
     2581 gctaggagtt aactttctca cttaaaagat taaggagata atatgtcatt aagaatcaat
     2641 aataatattg agtcgatgaa tgctcatcgc aacctgttaa tgaatgaccg tgcgctttcg
     2701 aagtctcttg aaagactggc ttcaggacag aaaatcaatc gggcatccga tgatccggca
     2761 gctttggtta tttctgaaca catgagggca cagatcagcg ggatggaaca ggcaatcagg
     2821 aataacgaag ttgctatttc actcgtacaa actgctgaag gttccatgaa tgaaatctcg
     2881 aacattctgg taaatcttcg ccagcgagca atttctgctg caaacgttgg tgccagtgac
     2941 caggatatga ttgacgccaa tcaggctgag attgaaaatg cgcttgcttc aattgaccgt
     3001 gttgtttcaa ccactcagtt cggacattac aaacttctgg acggaacaaa tgctgcgaag
     3061 acagagacaa atgaagacgg cagcatttcc actcaagaag gactgcgttt ccatgttgga
     3121 ccaaatgctg aacatatggc ttcaacctcc attagagaca tgagcacaag tcagcttggt
     3181 cgggttatag ttttagaagg tgaaatgcca aacaaaagca acttcatgtc actcgctgac
     3241 attgatgtgc gtaatgagca gggtgctcag gatgcacttg ctataattga tcaggctctt
     3301 acagaggttg ctacagttcg tggagaatta ggtgctttcc agaaacatac cctggaatct
     3361 aatctcacca gcttgcaggt cgcagcagaa aatatgactg ctgcagagtc tactattcgt
     3421 gatactgaca tggctcagga actggctaca tttacacgta accagatcat gactcaatca
     3481 gcaacagcac agttggctca ggcaaatgca atgccgcaac acgtcttgcg attgttaaac
     3541 ggctagcagg gttcaggggc agtttatccc ccagacataa cactattccg gctccattta
     3601 ttggagtccg gaatggctgt ttatattatt taaatgaacg tttatctata atcattattc
     3661 aaagatttgt cgatgactgg agtttatatt ccggttaacg ggatcaaaaa acttgaaact
     3721 taaatataac gaggaactat gccgttgggg atgaaacaca acattgcatc tatcaacgca
     3781 ataaatcatc tggccgaagc gcaaaatgaa ctgtccgatt cgctgaaacg attatcttca
     3841 ggacaaagag tcaacagtgg agcagatgat ccttcgggtc tgattctttc agaaggttta
     3901 cgcgctcagg ttgccagtgc gcaacaggcg ctgcaaaatt cggagttttc tctgtctctt
     3961 gtccagactg ccgaaggtgc tttggttgaa gtaaacaact tgttgattga aatgcgtcag
     4021 ttggctacaa ctgcagcaaa tgagggtgcc accgattaca atgcgctgtt agcattgcag
     4081 tttcagattc gtaaagcaat tgagggaatt gaccgtgttt cccgctttac tagctttggg
     4141 aataaaactc tgctagacgg aagccatggt gcaacaggaa tgggaggaaa tgaagaactt
     4201 gtttttctta aagcatcttc caaaacagtc gcttcacccg ttagcggata tgatgtggat
     4261 attaccgaat taccgctacg ggcaactttg acagcagacc ttgatgacga tgatgcttcc
     4321 ggtctgcaaa ttactttaga agaagaagat ggagctataa ttagagtcag taatccggaa
     4381 ggagccactg cagtcggatt tgcaaaacgt ctgcaaatag ctgtcttaga agcgaacatg
     4441 aatttgaaca ttcgctttga tgctgatgat gaagaactta caattgaaca tcgggaatat
     4501 ggactcacca aaggattcac tgtaaccgga acaaaagatg atgtaattgt tgacaacgct
     4561 tttgagccgg aattgctttt aggacgtgat atagaaggaa caattaatga tgagcctgct
     4621 gatggtgatg gactgatcct taccggcgaa tacaataatg agaaaaccag cggtttgagt
     4681 gtagcttttt taggtgatgg taccggcaat gccggatctg tgactgttgc gcaaaattct
     4741 cttaagtttc aagctggagc aagtgcagat gaaaaaattg taattgctct gaacagtacc
     4801 cattctactg tcctgggaag aggtgtggat aatacaagcg gatttgaaaa tttgtcccaa
     4861 atcagtctca agtctactca agaagccatt gatgcaattc gccttgtaga tgaggctctt
     4921 gatcaactat tatcaatgcg cagccagctt ggttctgtac aaaaacatac actggaaaca
     4981 aatatttcag ttctccgcag cacagtagaa aatttgactg cggcagaatc cagcatccga
     5041 gatacagaca tggctttgga aatggttaat tttacgaaaa accaaatcat tacggaagcc
     5101 gcagcagctg cagtcgcaca gtcaaatcag actgctacca gagttttgcg gctgttgttc
     5161 aacaataatc cacatggaca ctggtcattt tttagagacc actgatcgtt gatcaaataa
     5221 tgcgccttga cgatacaatt gctgcgatag caacaccttt gcagccatcc ggacttggag
     5281 tgatcagagt aagcggagag caggcagttt ctgcagtaga ggctctgttc aagacaaatg
     5341 ggaaaccgct gaaaaagacg gaaacacaca agctggtaca cggctggata aaagacgacg
     5401 gaaaacttat tgacaaagtt ttagtagtcg tcatgcgtgc tcctcattca tacaccactg
     5461 aggatgtggt tgaaattcag tgtcacggaa gcccttttgt gttgcgaatc atcctggatt
     5521 tggttttacg gcaaggagtg cgtttggcag gtgcagggga gttcacccaa agagcttttt
     5581 tgcatgggcg cctggacctg acacaagtgg aagcaatttc agacttgatt caagccagtt
     5641 ctgaaattgg ggcaagactg gctgcccaac aacttcgtgg aaaacttttt cacgccattg
     5701 atgctgtaaa aaaacaagtg gtctccattg cttcactagt tgaggccagc atcgagtttc
     5761 cggaagagga ccatgaattc actcatcagg aagattgtct gcagcgtctg gatcaggcta
     5821 gtgccgatct ggaaagctta ctttctcatg ctgaccgtgg aagacaaata cgtgatgggt
     5881 ttagtgtagt attgattggc cggccgaatg taggaaaatc ttcgcttcta aataccttgc
     5941 ttcgcgagca aagagcgatt gtaaccacca ttcccggtac aaccagagat tcaattgagg
     6001 aatctgtaca aattcagggt ctagcttttc gcttaacgga cacagccggc attagaaaaa
     6061 cagaagacct gatcgaatca gaaggtattc ggcgtacaca aaatgtccgc gaaaaagccg
     6121 acctcgtctt gttgattctt gatgccagtg aaaaacttat ggacgaagat ttgagtttaa
     6181 tcagtgaagt tgaaaaagag cggacaattg tagttctcaa taaaaaagat ctaatggacg
     6241 acactacccc gcgttggtat gaaaaaatat cccgtttgga atctgttttg atttcagcaa
     6301 aaacaggtga aggttgtgat gagttggaat ccagcttata taaaaaagct acactcggcg
     6361 gtttgcctca ggaagatgaa atctggatca caaaccgcag gcagcaacag gctgcaaaaa
     6421 atgctctgtc tgctttgttt tcagcgcgaa aaagtttgaa tcagtcaagt ggagaagagt
     6481 ttctcgcagt tgatttacgc tcttgtttaa acgccctggg tgaaatagtt ggcgaaacaa
     6541 cgcccgatga tttgcttggg caaatatttt cggatttttg tattggaaaa taaacaaatg
     6601 acagacagcg aattgaaact actgcttgaa aaacaagagt tacttttaaa gaagcttctg
     6661 gaattatcac agcgccaatt tgcagaatcg gatgcggtcg cactggacga gctcttgaaa
     6721 caaaaagata gctactttga tgaactgcaa aagttagatc ctttgcggga aaagtggcac
     6781 aataaataca accgtccctt agggcaggaa gaacaaaaac ttgatgataa tattcaggat
     6841 ttacttgaaa aactactgct ctcagaacag gattttgaaa aaatagtagg ccgtgaaaaa
     6901 aatgctgtct cattacaaat tgctcaaata agcaatcaaa tgcagtatcg aaaagatact
     6961 actaggcaaa ggcctcaaat taaaaacatg acaacttaaa cacaacatta ttcatagtga
     7021 acaatgcttt tttctgctta ccaaattgag tccctgttag ccgattttca acagacagac
     7081 tctccagcag gcaaatctgc cagcgaaact ctaagtcttt ctgctgatgc gattcaggac
     7141 gcacgtcaat acttgcgccc actgaaacgc agtcccctga actgggttac tcccaaagat
     7201 attaggggac tggtcgaaga catttcttct tacgatcctg ttgtagataa tgtgcagcag
     7261 agtcgtgccg gccgttttgt tgtacctgct tatgtacctg aaattcctgc accagcgttt
     7321 ccacttgtaa gcgaccacac tcagatggcc ccggaaagtg aaactacaga gcaggaatca
     7381 tcggaaagtc ctgttacaga agaagatgct ctgatgcccg aaataaacac tgaatcatct
     7441 gaagtcgaag ataaggtttc ggtcagtgtg tctgaggata tttcgaaaga aaatagtctg
     7501 ataatagaaa ctgagaaaag ttctgaagaa tcagaagaat ctaccaacgg cgaatcggca
     7561 gtttctactg cttcattatt tgaaggtatt gatgatgaag aacatgatga agagcatgaa
     7621 cttgaagaag aaggattgca aggagacaat agtgaggaaa acgatgtcgt ctttggagat
     7681 ggccgcattg atcaaaaatc aatgagcaat tttgtagctc attatcctga cagtactctt
     7741 aagttcctga tgcgtaaaaa tttggacggt agaccattac cggttggata cgaagaaata
     7801 tattcacaat gggaaaatcg cggattgtcc cgtggacgcc tgaaaaaata tttatttaaa
     7861 ctgatggaat ggaaaaactt tccggatatt cctgttcatg atgtggttaa taaaatccgc
     7921 gaacatcaat attttcttga aataaagtaa tgacagcaaa aaggttcaga ccaaatgttg
     7981 cggcggtgat aatgcatcca tcaaataaaa aagttctgat gttcaggcga attgccaaaa
     8041 gaaatgaaaa atcagaacta ttgtcagatg gaggacagtt aagatggaat tggcaattcc
     8101 cccaaggagg gattgaccca ggggaatccg aagaagagac actttttcgt gaacttgaag
     8161 aagaaattgg caccaatgat gttacaatca tccgggttgc tgaaaaaaga gtccgttact
     8221 actatcctct caaaatgctg catattcttc ataaacaacc tgaatggaaa ccttaccgcg
     8281 gccaacagca acgctggttt cttctacagt tgaattgtga cactgagaaa attaattttt
     8341 cccatcaacc cattgaattc gatgccttcc aatggatctc accccgcctg ggattaaaga
     8401 aagttgttcc tttcaagcgc aaggcatacc gtaaaggttt gcgatatttg gggattttgt
     8461 gatttgattt gatttgctca taaaaacagc agataatctg aagctcactc caaaaataat
     8521 cgttttgaat ttgaacacgt ttgctatact gagtaaactg caattgttta aatttcaatg
     8581 cacgtatata attatatcat taaaggaaat aatcatataa aggcaattca atgctaatgc
     8641 tcctttcacc gtctaaaacg ctggattatg aaactcctgc gactactgac agcttcagta
     8701 ttccggaata tctggaaaaa tcttctgaac tggttaaagt tctgaagcag aagtcttttc
     8761 tggatttgat ggaactgatg caggtcagtc aaaaaattgc tgagcttaat gttgaacgtt
     8821 ttaatcaatg gaaattacct ttttccaccg aaaatgccaa acaagccgtt ctagctttca
     8881 aaggagatgt ttatacgggt cttgacgcac ctgcactttc tgaaaaccgt ttggcttata
     8941 cccagtcgca tctgcgcatt ttgtcaggac tttacggttt gctgcggccg cttgacctga
     9001 tgcagcccta tcgcctggaa atgcggctga agctgacaac gaaaaaaggt gaaaatttgt
     9061 atcaattttg gggtgagaag attactgatg cattaaatgt tttattggca aaacaagatg
     9121 agccggtttt aatcaacctt gcttcaaatg aatactttaa atcagtacaa aagaagaact
     9181 tggacggtcg tctaattaca cctgagttta aagactggaa aaacggcaaa tataaaatga
     9241 taagtttttt tgcaaaaaaa gctcgaggtt taatggtgcg ctatgccata gatcacaata
     9301 tacagaaagc agaagtatta caaaattttg attatgatgg ttaccatttc aaccttgaac
     9361 taagtcaggc agataaatgg gttttcagca gaggctgaaa atttgactcc tgttttttta
     9421 caacatgaga ctgctgagtc ataattcgtt ggagaaaaat tacctctctt tattaggtct
     9481 aacaacttcc cccttcattc tactaaattc cactgtcatc actggaattt aaatcatttt
     9541 gaatcaactt caatttctat tcctcccaaa taatagacct tcaaagcaga aaagggcaat
     9601 ttattgcttg ctttaagata taaatcattg tattgatata tgatgattta a
//
LOCUS       DTVD01000085            2394 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_18867_length_2394_cov_394.376657, whole genome
            shotgun sequence.
ACCESSION   DTVD01000085 DTVD01000000
VERSION     DTVD01000085.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18867_length_2394_cov_394.376657"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(6..305)
                     /locus_tag="EYO68_03030"
     CDS             complement(6..305)
                     /locus_tag="EYO68_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIC06809.1"
                     /translation="MLGFETVDDGEIFHTVKFIEDVPLIMIVEIGRTEMEVQKVLNLK
                     KGSMLVFEKIVGEPMDVVVGNRLMARGEIVVVNERYGVRISEVTRPDEKIGARRE"
     gene            complement(447..2282)
                     /locus_tag="EYO68_03035"
     CDS             complement(447..2282)
                     /locus_tag="EYO68_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HIC06810.1"
                     /translation="MRICQGKAMNSVQETIYDESKILTLSSLEHIRLRPGMYIGRLGN
                     GSHPSDGIYVLLKEIVDNSIDEFIMGFGKRIDIRVENGAVNIRDFGRGIPLGKLVECV
                     SIINTGAKYNDEVFQFSVGLNGVGTKAVNALSDQFEVTSFREKKSLFAQFKSGELKND
                     PATNDTNESNGTKIRFTPDSKLFPNYCYDLGTIRKQLWNYAYLNRKLTITLNGEKFKS
                     ENGLLDLLKEEVSEANLYDIVHCQNGQLEMALTHTEHYGEEYFSYVNGHHTNDGGTHL
                     SAFREGILKGANQFFNASFDGPDVRDGLVGAVAIKVQEPIFESQTKNKLGNSELRSWL
                     MPLVRDTIVDFLYKHPEVAEPLKQKIQTNERIRKELNNVKKAARENAKKSALTIPNLK
                     DCKFHLGDKSERGDESTVFITEGMSAGGSMISSRDVYTQAVFCLKGKPLNCYGQGKEA
                     IYKNLELYNVMKALGIEDSLEDLRYNKVVIATDADVDGLHIRNLLLTFFLQFYEPLVL
                     QGNIYILETPLFRVRNQKTTQYCYSETEKDEAFEKLQKGTHFEVTRFKGLGEISPKEF
                     GQFIGKNMRAVPVGVEHTHEVPELLNFYMGTNTAERRQYIMENLV"
BASE COUNT          627 a          543 c          463 g          761 t
ORIGIN      
        1 gctttttact cgcgtcgtgc gcctattttt tcgtcaggac gagtgacctc actgatgcgc
       61 acaccatagc gctcattcac cacaactatt tctccacgtg ccattaggcg gttgccaaca
      121 actacatcca ttggttcgcc tactattttt tcaaacacca gcatactgcc ttttttcaga
      181 ttaagtactt tttgtacttc catctctgtt cgtccaattt cgacgatcat aatcaaaggc
      241 acatcttcaa tgaattttac cgtgtgaaaa atctctccat catcaacggt ttcaaatccc
      301 agcatcagcc gttcctctgc aattagtttt tattctatag tgtatgtgag gttcaaaagc
      361 aggccaacct ttgagcggat tggcactgag tttatgtgaa cagtttgtgc agattatgtg
      421 cctctttccg aagtatccgc aagacctcaa acgagatttt ccataatata ttgacgacgt
      481 tctgcagtat tggttcccat gtagaagttc agtaattctg gaacttcatg tgtatgttcg
      541 acaccaaccg gaacagcgcg catattttta ccaataaact gtccgaactc ttttggtgaa
      601 atttcaccaa gtcccttaaa gcgcgttacc tcgaaatgag tccctttctg cagtttttca
      661 aatgcttcat ccttttcagt ctcgctgtaa caatattgtg ttgttttctg gttacgaaca
      721 cgaaagagag gtgtttccag aatgtaaatg ttaccttgta atacaagtgg ttcgtaaaat
      781 tgcaaaaaga aagtcagcag taaattacgg atatgaagac cgtctacatc ggcatcagta
      841 gcaatcacaa ccttgttgta tcgcaaatcc tcgagtgagt cctcaattcc gagggccttc
      901 atgacattgt acagctcaag atttttgtaa atagcttctt tgccctgtcc ataacagttg
      961 agcggcttcc ccttgagaca gaaaactgcc tgtgtataaa catcacgtga gctaatcatg
     1021 cttccgccgg cagacatgcc ttcagtgata aaaacggtac tttcatctcc acgttcgctt
     1081 ttgtcaccaa ggtgaaattt acagtctttt agattgggaa tcgtcagtgc tgattttttt
     1141 gcattttccc tggcagcctt tttaacattg ttcaattcct tgcggattcg ttcattagtc
     1201 tgtatttttt gtttgagagg ttctgctact tccggatgtt tataaagaaa atcgacaatt
     1261 gtatcacgaa ccagcggcat cagccacgaa cgcaactcgg agtttcccaa tttattttta
     1321 gtctgtgatt caaaaatcgg ctcctgtact tttatggcta ctgcaccaac cagtccgtct
     1381 cttacatccg gcccgtcaaa gcttgcatta aaaaattggt tggctccttt gagaatgcct
     1441 tctcgaaatg cagaaaggtg ggtaccaccg tcgtttgtgt gatggccgtt gacatagctg
     1501 aaatattcct caccgtaatg ctcagtgtgt gtgagcgcca tttctaattg tccgttttga
     1561 cagtgaacga tgtcataaag atttgcctca cttacttctt ccttcagtaa atccaatagg
     1621 ccgttttcgg acttaaattt ttcaccattc agtgtgattg tcagcttacg gtttaaataa
     1681 gcatagttcc agagttgttt tcgtatagtg cccaggtcat agcagtagtt tggaaaaagc
     1741 ttggaatcag gtgtaaaacg aatttttgta ccgttactct cgtttgtatc atttgtagct
     1801 ggatcatttt taagttcccc ggatttaaac tgggcaaaga gtgatttttt ttcacgaaaa
     1861 gaagtgactt caaattgatc tgaaagagca ttcactgctt tggtgcccac tccattcagc
     1921 ccgacactga actgaaaaac ctcatcattg tatttcgcac cggtgttgat gatggaaaca
     1981 cactccacta acttccctaa aggaatccca cgcccaaaat cacgaatgtt tactgctcca
     2041 ttttctacac gaatatcgat gcgcttcccg aagcccataa tgaattcgtc aatggaatta
     2101 tctacgattt ctttaagtaa aacgtagatt ccgtcactgg ggtgggatcc gttgcccagg
     2161 cggccaatgt acatgccggg gcgtaaacgg atatgttcaa gggaggaaag cgtgaggatt
     2221 ttgctttcgt cgtatatcgt ctcttgtact gaattcattg cctttccttg acagattcgc
     2281 attccatgct ttccctcaac ttacaaggtg agggaatttg ccgagctggt aaaaaacttt
     2341 ttcccgacaa agttaaccct tcaaatatat gcaatcatca tgaaattgtc aaaa
//
LOCUS       DTVD01000086            9498 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1896_length_9498_cov_426.234777, whole genome
            shotgun sequence.
ACCESSION   DTVD01000086 DTVD01000000
VERSION     DTVD01000086.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_9498_cov_426.234777"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(382..1554)
                     /locus_tag="EYO68_03040"
     CDS             complement(382..1554)
                     /locus_tag="EYO68_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06811.1"
                     /translation="MKSLFSFVFLILFFCLPLPAEEVIVKGSAKIYNGDQTHASEQAL
                     KNALLEVVKRGVESILDDRTISLNYEVIKNQIYRDRQKYIRNYEIIREEPVLNGTNYE
                     IQIRAKVEKVKIEQKLKVLRILHDRMLNKRLLFVYHRSNPEAVLRDNTAVENALTPVQ
                     KTFAEHGFRTFGEQTMKQVYSSLEQETLVGSSVDSLIALALNHNADVLVVMEMIAVKP
                     YKTSGTLFKVGSKVHFRLYEAVTGKQIAETIVVQNEVSIKKPDDHKWNVLFGKAANQA
                     VMESIRQSTEYIIRFYQNTNLAGQEYSVVFKGYSPRSQIMIVEYLENTTAFRKVSELK
                     NTFGFLEIELNTLMHKSTLRRRITSDLLVQEIEVATKSLAGNQLVFINPNPMEEED"
     gene            complement(1661..2968)
                     /locus_tag="EYO68_03045"
     CDS             complement(1661..2968)
                     /locus_tag="EYO68_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06812.1"
                     /translation="MRFLISAFSNQFFRISLRAALLPAVILTAGCSVTRIYHEVPPSP
                     DALSEINSFHVNQVEGVQSALFGKILIHELDQFPQLNYLAIFPEVSKTNAAVLSAEVR
                     RYSTNDEEKTLTQTHISLVEHKVLQENPSGLNVFRRTFEFEEKTYSERIIQRTSDLEI
                     AFKVTNIAGDKTLYFKVENASIEQSYRGDENILLIPDSDDAMIHLAQLLIRKFLDRIN
                     PEQNEHVVELEKGTNPVPFTFGLLDFGHPRIIRSNHFATGNRYDLALKGWNYVLFEPR
                     TYPKFERFIFTDEVYIRLKKSGLPQTTLRPLLEIHGKSFDHNEINIVLSGLIPNQDFE
                     RYAQIIKSHSRFSQNINRLNYAAAHYNLGVVFQLRNELELAAYHFSQANAYNPQEKYS
                     QAWTDLQHLKGEYNPLASMMDHSVESYGKLPPPEGALLQPKTN"
     gene            complement(2970..3257)
                     /locus_tag="EYO68_03050"
     CDS             complement(2970..3257)
                     /locus_tag="EYO68_03050"
                     /inference="COORDINATES: protein motif:HMM:PF16732.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06813.1"
                     /translation="MQRSKQTEAKVGLSAVYTAQKIYFAINQTYADSLSNLDVQLETG
                     GSSRYSITLTGSSSSFTATAKGNLDDDAVLDIWTIDQNKTLQNTVSDITSE"
     gene            complement(3258..3368)
                     /locus_tag="EYO68_03055"
     CDS             complement(3258..3368)
                     /locus_tag="EYO68_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC06814.1"
                     /translation="MSRMKRLRRGFSLLEVMIVVVIIGILATLAYPSLEG"
     gene            complement(3521..4978)
                     /locus_tag="EYO68_03060"
     CDS             complement(3521..4978)
                     /locus_tag="EYO68_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIC06815.1"
                     /translation="MEFIKLTDLVPIIPEIIVLLTACTVLLVDLFASKKDRAFTLAVT
                     TIFGLVLAMLSETQLHNRDLSGFYGTVVADDFSVLFEFIYMSVAILTVFVSQHYIAEN
                     EMNFGEYYVLLLTSVSGMMFMTSGLDLLVIFIGLEIMSISSYILVGMKRKVAEANEAA
                     LKYLLLGAFSTGFLLYGISLLYGATGSTLLPEIIAEIKQNGADNPLVVVGTALVIIAM
                     SFKIAVVPFHMWTPDVYTGAPTPVTGFLSGAAKAAGFAVFVRILLTGIPLESANWENL
                     LWILAVLTMTVGNVMALRQNEVKRMLAFSSIAHAGYVMVAVVVGSTSAISGVIFYSCA
                     YALMGTGAFGILTIRDAGTIPKTFDDLAGYGKRNPFLALLMTLFIFSLVGLPLTGGFI
                     GKLQIFSAALQDGWIWLTVIGILNSALSVYYYLRVVMYMYMREGALPEGSDDSNKTYG
                     FALTGLIGTAAAVLYLGVFPDQILELASESIRALL"
     gene            complement(5075..6574)
                     /locus_tag="EYO68_03065"
     CDS             complement(5075..6574)
                     /locus_tag="EYO68_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIC06816.1"
                     /translation="MSNIIGNHVAGSRLSQTGGSLQDVFNPSTGDVSGQIRLSSAAEL
                     DDVVKVASEAALGWGATPPMKRAQVMFRFKRLLEENADKIASTISSEHGKTHQDALGE
                     VSRGLEAVEFSCGIPHLLKGTFSEQAAASIDVYSIPQALGVVAGITPFNFPAMVPLWM
                     IPNALACGNSFILKPSERDPSVTLILADLLKEAGLPDGVFNVVQGDKVVVDAILDNPD
                     IMAVSFVGSTPVAEYIYTRGAANGKRVQALGGAKNHMIIMPDADMGQVVDALMGAGYG
                     SAGERCMAVSVPVPVGEKVADDLMDRMIPLVENLPVGPPADTNAAMGPVITRQSLDRI
                     TSLLQSGIDKGAKLLVDGRGVKVPGHENGFFIGASLFDHVTPEMDIYKEEIFGPVLST
                     VRVTDYEAAIKLVHGNPYANGVAIFTRDGDAARDFVNRIEVGMVGVNVPIPVPVGYHS
                     FGGWKKSIFGSHHIYGPDTIHFYTRLKAVTSRWPAGIKAGAEYNFPMMS"
     gene            6796..9468
                     /gene="gcvT"
                     /locus_tag="EYO68_03070"
     CDS             6796..9468
                     /gene="gcvT"
                     /locus_tag="EYO68_03070"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIC06817.1"
                     /translation="MTENKDTAEQLLRTSLHSWHTLNGGKMVDFGGWDMPVQYSTGIL
                     KEHLATRRYGGLFDVSHMGRLRILGKDTVRFLQHVLTNNAESLDTWQAQYTLIPNENG
                     GLLDDAYLYHPGEEYFLVVNASNREKDWNHFQEQAKDFDVQLEDETNEVAMIAFQGPL
                     SGRILTRVKRDGTMPETFRNSLSKITILGTEVLVARTGYTGEPLGFELFIPAGKAEAI
                     WARIEEEGKDEGVVAVGLGARDTLRLEAGMPLYGHEFGIDTEGREIPAFAFPLTSVAV
                     SFSKRKGDFVGRAALKAQFEEVRKLRMGQYEPSDVLQRRFLPLALSDKGITRGGDSVF
                     LDDRKVGVISSGTMVPYWKFEGESATMQITDEQERRALALVYVDATVPVEQELEVEVR
                     GRRLSAQLVPWHGRSEAPPYFRAIPIDRKPDSAVATSAAVREKTVLLLKKSLDNHEWR
                     QRRCVNLIPSEMTQSSLVRLLQITDPCGRYAEHKELLAAFEQEVFYYQGTEFIAWAED
                     RLVEEMQSFLGFPLVETRVISGQMANMTIYSALVDFLNRTDRRREPRRIPLVMNNHIG
                     KGGHLSSQPMGALRDYVAKDPVTEKYSVLNFPVLQDNPYRIDLEETANLLDRFDPGLI
                     ILGKSMILHPEPVADIRKMLDTKSTRPVIMYDMAHVLGLIGPHFQNPFAEGADIITGS
                     THKTFYGSQRGVIGAAYEEGAPEFELWKAIERRAFPGAVSNHHLGTLLGLLMATLEMN
                     AFKETYQPQVVANAKAFAIALADEGLEVQGDPEVGYTETHQVVVVVGYALGCAVAQEL
                     EESNIIVNFQAIPSDESFTSSSGLRMGVAEMTRFGMKEDDFVEFAAIFNEAVHGRNVG
                     DEVARFRERFQTLHFCFDADYPEYLNNLSGLNLV"
BASE COUNT         2682 a         2113 c         2091 g         2612 t
ORIGIN      
        1 atgtttgata aaccagttta taatcaaaat tgcatagggc tcactgaaaa agtttttctt
       61 ttgtcgtgcc ggacttgatc cgggatcaag atcaccgacc agcttgagta caagatttga
      121 aacatttccc ctggattacg ggtctatgct gcacttcgcc gaatgacaaa tgagaataat
      181 ctaaagtagc acaactatta ttttcgtggg gctgccttgt tgggaaaaga gactcaaacg
      241 acagaagtgc agggccttcg cgcttttcag gaaacacccc gaagttgtcg taataaatcg
      301 aataattact tatttatatt attgagaagt ggactgttcc acagattcgc tagaaatatc
      361 tgaagagttg tctgaagaaa cttaatcttc ctcctccatt ggatttggat tgatgaaaac
      421 cagctgatta ccagctaaag attttgtggc aacctctatt tcctggacaa gcaagtcact
      481 ggttattctt ctgcggagag tacttttatg cattaaagta ttcaattcaa tctccagaaa
      541 accaaaagtg tttttcaatt cagaaacttt tctgaaagca gttgtatttt ccaggtattc
      601 cacaatcatt atttgactcc gtggagaata acctttgaaa actacagaat attcctgacc
      661 tgccaaattt gtgttttgat aaaaacgaat tatatattct gttgactggc gtatactttc
      721 cattactgcc tgattagctg cctttccaaa taaaacgttc cacttatggt catccggctt
      781 ttttattgaa acttcatttt gcacaacaat tgtttcggca atttgtttac cggtcacagc
      841 ttcataaagc ctgaaatgca cctttgatcc gactttaaac aatgttccgc tagttttgta
      901 cggtttgaca gcaatcattt ccattaccac cagaacatct gcattatgat tcaatgccaa
      961 cgcaatcaaa ctgtctacag aactaccgac taaagtctcc tgctcaagtg agctgtatac
     1021 ttgcttcata gtttgttctc caaacgtccg gaagccgtgt tctgcaaaag ttttctgaac
     1081 tggagtaagt gcattttcta cagccgtatt gtcacggagt acagcctcag gattacttct
     1141 gtggtaaaca aacaacaaac gtttgttcag cattcgatcg tgcagaatac gcagtacctt
     1201 cagcttttgc tcaatcttta ctttctcaac tttagcccgg atttggattt catagtttgt
     1261 cccgttcaga acaggttctt ctctgattat ttcataattt ctgatgtatt tctgtctgtc
     1321 tctgtatatt tggtttttaa ttacctcata attgagactg attgtcctgt cgtccagaat
     1381 gctttcaact cctcttttaa ctacttcaag caaggcattt tttaatgcct gttcacttgc
     1441 atgagtttgg tcgccgttgt atatttttgc agaacctttg actattactt cttctgccgg
     1501 caaaggaagg caaaagaaca ggatcagaaa cacaaaggaa aataatgatt tcatgaatat
     1561 aataactgaa cagtatacaa acagtgaatg gacaaattct aaaacaattc cgaactatga
     1621 aatgtaaacc gaaatcagtt ttgaatcaag actaaggaga ttaatttgtt ttcggctgca
     1681 gtaaggcacc ttcgggagga gggagttttc cgtatgattc aactgagtga tccatcatgc
     1741 tggccaacgg attatattcg ccttttagat gctgcaaatc agtccatgct tgagaatatt
     1801 tttcttgagg attgtaggca tttgcctgag aaaagtgata tgcagccaat tcaagttcat
     1861 ttcgtaattg aaaaactaca cctaaattgt aatgtgctgc agcataattc aaacggttga
     1921 tgttctgaga aaatcttgag tgtgatttta ttatctgcgc ataacgttca aaatcctgat
     1981 taggtattaa gccagaaagg acaatattaa tttcgttgtg gtcaaacgac ttgccgtgga
     2041 tttcaagtaa aggccgcaga gttgtttgag gcagccctga ttttttgaga cgtatatata
     2101 cttcgtctgt aaaaataaag cgctcaaatt taggataagt acgcggttca aaaagcacat
     2161 aattccagcc cttcagtgca agatcgtatc tgttgccggt agcaaaatgg ttggaacgta
     2221 ttattctagg atggccgaaa tccagcaaac cgaatgtaaa aggtaccgga ttggttcctt
     2281 tttccaattc aacgacatgt tcattctgtt ccggatttat tctgtctaaa aattttcgaa
     2341 taagcagttg ggcaaggtgt atcatggcat catcggaatc tggaataaga aggatgtttt
     2401 catcacctct ataactttgc tcaatgctgg cattttctac tttgaagtag agagttttgt
     2461 ctccggcaat atttgtgact ttaaatgcaa tttctaagtc cgatgtccgc tgaattatcc
     2521 gttcagaata ggtcttttcc tcaaactcaa atgttcggcg aaagacattg agtcctgatg
     2581 gattttcctg cagtactttg tgttccacca aactgatatg ggtttgagtg agggtctttt
     2641 cctcatcatt tgtggaataa cgcctgactt ctgcactcag tactgctgca ttcgtttttg
     2701 aaacttctgg aaaaattgct aaataattca gttgggggaa ttgatctaac tcatgaatca
     2761 ggattttccc gaacaatgct gattgcacac cctcaacttg attaacatgg aaggagttta
     2821 tctcagaaag tgcatctggt gacggaggga cttcatgata gatccgggtt accgaacaac
     2881 cggctgtaag tatcactgcc ggcaacagtg cagctcgaag agagattcga aaaaactgat
     2941 tgctgaatgc agaaatcaga aacctcattt tactcagaag tgatatcgct gaccgtattt
     3001 tgcagggttt tgttctggtc aattgtccaa atatccagca cggcatcatc atcgaggttg
     3061 cctttggcag ttgcagtgaa ggaggaactg ctgccggtga gagtgatgga atagcgggag
     3121 ctccctcctg tttccagctg tacatccaag ttactgagtg aatctgcgta agtttgattg
     3181 attgcgaaat atattttttg tgcggtgtaa actgcactaa gaccaacttt tgcttcagtt
     3241 tgttttgagc gctgcagtta gccttccaaa cttggataag cgagtgtggc cagaatgccg
     3301 attattacaa ccactatcat cacttccagc aaactgaatc cccgacgcaa tcttttcatc
     3361 cttgaaatta tgcaaaaagt atggccattt ttcattacct tcctccaaaa gacaaaaaaa
     3421 gagactgtac atttcgttgg ggaacaaata cctgttttat tttgttacag gcaattattt
     3481 tcaaactact atattgtcct atctttattt aagcagcgac ctagagtagc gcccgaatgg
     3541 actcagaagc aagttccaaa atttggtcgg gaaaaacacc aagatagagc actgctgctg
     3601 ctgtaccgat taatcctgtc agggcaaatc cgtaagtttt gtttgaatca tctgagcctt
     3661 ccggaagtgc accttcccgc atgtacatat acatcaccac cctcaaataa tagtaaaccg
     3721 aaagggcgct gttaagtatt ccgattacag tcagccagat ccacccatcc tgaagtgcgg
     3781 cactgaatat ttgaagtttg ccgatgaatc cgccggttaa cggcagtcca accaatgaaa
     3841 aaatgaataa tgtcatcagc agtgcaagaa agggatttcg tttaccataa cccgccaaat
     3901 catcaaaagt ttttgggata gtcccggcgt cacgaatggt taaaattcca aaggcaccgg
     3961 ttcccatcaa agcatatgca caggaataaa aaataactcc gctaatggca gaagtgcttc
     4021 ccaccacaac tgcaaccatc acatatcctg catgtgcaat cgaagaaaaa gccagcattc
     4081 gttttacttc attttgacgc agtgccatga cattaccaac tgtcatggta agaacggcca
     4141 gtatccataa taaattctcc cagtttgcgc tttcaagagg aataccggtc agcaaaattc
     4201 gaacaaatac tgcaaatccc gcagctttag ctgctccgga aaggaagcct gttaccggag
     4261 ttggtgctcc ggtgtagaca tccggcgtcc acatatgaaa cggaacaact gcgattttga
     4321 aactcatggc gataatcacc aatgctgttc caactacgac aagaggatta tcggctccat
     4381 tttgttttat ttctgcaatt atttctggca gcagggtact gccggttgca ccataaagca
     4441 aagaaattcc atagagcaga aaacctgtag aaaatgctcc cagcagcaag tatttgagtg
     4501 cagcttcatt tgcttctgca actttgcgtt tcattccgac cagaatatag ctggaaatgg
     4561 acatgatttc caggccgata aaaataacga gtaaatccag tccggatgtc ataaacatca
     4621 ttccggaaac tgaagtgagg agaagtacat aatactctcc aaaattcatc tcattttccg
     4681 caatataatg ttgtgaaaca aacaccgtca ggattgcaac gctcatgtaa atgaactcaa
     4741 aaagcaccga aaaatcatcg gcgacaaccg ttccgtaaaa ccctgaaaga tccctgttgt
     4801 gcaactgagt ttcagaaagc attgccagga caagaccgaa aatcgtagtt actgccaatg
     4861 taaaggctct gtctttttta gatgcaaaga gatcaacaag caaaactgtg caggcagtca
     4921 gcagcactat gatttcggga ataatcggaa ctaaatctgt aagcttaatg aattccatta
     4981 ttttctcagg aatttgactc tcccggcatt ggaagagcaa agttgttttg atttaccaaa
     5041 aaagacgttg cggaatgaaa catccaccac accctcagga catcattgga aaattatatt
     5101 cggctccggc tttaattccg gcaggccagc gtgaagtaac tgccttcagg cgcgtgtaaa
     5161 aatgaatggt atcgggccca taaatgtgat gacttccgaa aattgatttt ttccaaccac
     5221 caaaactgtg gtaaccgacc ggtaccggaa tcggcacgtt aaccccaacc ataccaacct
     5281 caatccggtt gacaaaatct cttgcagcat ctccgtccct ggtgaaaata gccacaccat
     5341 ttgcatatgg attaccatgt acaagtttga ttgccgcttc ataatctgta actcgaacag
     5401 tggataaaac cggcccaaat atttcttctt tataaatgtc catttccgga gtaacatggt
     5461 caaaaagaga ggctccaata aaaaatccgt tttcatgtcc gggaactttt actcctcttc
     5521 cgtccaccag gagtttcgcc cccttatcta ttccgctttg aagcagtgaa gtaattctgt
     5581 ctaaactctg tctggtaata accggcccca ttgcggcatt ggtatcagct ggaggtccaa
     5641 cgggcagatt ttcaaccaaa ggaatcatcc gatccatcaa atcatccgca actttttctc
     5701 caaccggcac aggcacagaa acagccatgc agcgctctcc ggcagagccg tatccagctc
     5761 ccatcaaagc atcaacaact tgccccatgt ctgcatcggg cataattatc atgtggtttt
     5821 ttgctccgcc caaagcttga acacgcttgc cgttagcggc tccacgagtg taaatatatt
     5881 ctgcaacagg agtcgaacca acaaaactta ctgccatgat gtccggattg tccaaaatcg
     5941 catctaccac aactttgtca ccctgtacca cattgaacac tccatcaggt aaacctgctt
     6001 ctttgagcaa gtctgctaaa atcaaggtta cacttgggtc acgttcagac ggtttcaaaa
     6061 tgaaagaatt tccacaggca agagcgtttg gaatcatcca caatgggacc attgcaggaa
     6121 aattaaaagg cgtgataccg gcaactactc caagcgcttg tggaattgaa taaacatcaa
     6181 tcgaagcagc agcctgttca ctgaaagtgc ctttcagcaa gtgcgggatg ccgcaggaaa
     6241 attcaactgc ttcaagtccg cgagatactt ctcccagagc atcctgatgt gtttttccat
     6301 gttcgctgct gattgtgctc gcaattttat ctgcattttc ttccagcaaa cgtttgaaac
     6361 gaaacattac ctgtgcccgt ttcatcggag gagttgcccc ccatccgagt gctgcttcac
     6421 ttgcaacttt taccacatca tcaagttctg cagcacttga caatctaatc tgtcctgaaa
     6481 catcgccggt ggaaggatta aaaacatctt gaagagatcc acctgtttga ctgagccttg
     6541 agcccgcaac atggttcccg attatgttac tcataacatg cctctgaaat aatttatcaa
     6601 aaaataattg tgttgtaaaa aagtacaatt ctatggaaaa tagtcgtgcg aatcaagttt
     6661 ttttgcttta aagcacaaat gatttttgtg taagcccaat actactcgcc ttgcttcaat
     6721 attttttcct gaatgcttta gttcagctca gaaacactgc ttttcaaaat aacaaattat
     6781 cactttaaaa tatttatgac agaaaataag gataccgcag agcaacttct acggacttca
     6841 ctccattcct ggcacacact gaatggagga aaaatggttg attttggcgg ctgggacatg
     6901 cctgtgcaat attccacggg aattctaaag gagcatctgg ccacaaggcg ctacggcggg
     6961 ttgtttgacg tcagtcacat gggacgcttg cggattctag gcaaggatac cgttcggttt
     7021 cttcagcatg tcctcaccaa caacgccgaa tctctggata cgtggcaggc tcagtacacc
     7081 ctgatcccaa acgaaaacgg aggactgctg gacgacgcct acctctatca tccgggagaa
     7141 gaatattttc tggtggtaaa cgcatctaac cgtgagaagg actggaacca ttttcaggag
     7201 caggccaagg actttgatgt acaacttgag gatgaaacca atgaagtggc aatgatcgcc
     7261 tttcagggtc ctctttccgg acgaattcta accagggtaa aacgcgacgg aacaatgcct
     7321 gaaacctttc gcaacagcct ttccaaaatt acgattctag gtacagaagt gttggtagcc
     7381 cggacaggtt acaccggaga accactggga tttgaacttt ttattccggc aggaaaggca
     7441 gaggctatct gggcgcgaat tgaagaagaa ggtaaggacg aaggagtggt tgctgtagga
     7501 ttgggtgctc gtgacaccct gcgtctggaa gccggtatgc cgctctatgg ccacgagttt
     7561 ggaattgaca ccgaaggcag ggaaattccg gcctttgcgt ttcctctaac gtcggtggcc
     7621 gtaagttttt caaagcgcaa aggcgatttt gtcggacggg ctgcattgaa ggcgcaattt
     7681 gaagaagtgc gtaaactacg gatgggacag tatgaaccaa gcgatgtact tcagcgccgc
     7741 tttctgccgc ttgctctgag tgataaggga attacgcgtg gtggcgattc agtattcctg
     7801 gatgatcgaa aagttggagt gatttccagc ggaaccatgg tgccttactg gaagtttgag
     7861 ggcgagagtg caacaatgca gattacagat gagcaggaac ggcgggcact cgctttggta
     7921 tacgtcgatg ccactgtgcc tgtggaacag gaactggagg ttgaggtccg cggacgaagg
     7981 ctgagtgcac aactggttcc atggcacgga cgctccgagg ctcctccgta ttttcgggca
     8041 attccaatag accgaaaacc ggattcagca gtagctactt cggccgctgt cagggagaaa
     8101 acggtactgc tgctgaaaaa aagtttagac aatcatgagt ggcgtcagcg ccgttgcgtc
     8161 aacctgattc cgtctgagat gacacagtct tcgctggtta gactgctaca gatcaccgat
     8221 ccctgtggac gttatgctga acacaaggaa ctgcttgcgg cctttgaaca ggaagtcttt
     8281 tactatcagg gaacggagtt catcgcctgg gcagaagatc ggctggttga agaaatgcag
     8341 agctttctgg gcttccctct agtggagacg cgggtcatca gcggacagat ggcaaacatg
     8401 actatttaca gtgccctcgt tgattttctt aaccgcacgg accgccgaag agaaccccgc
     8461 cgcattcctc tggtgatgaa caaccacatc ggtaagggag gtcacctcag ttcacagccg
     8521 atgggggcgc tgcgggatta tgtcgcaaag gatccagtca cagaaaaata ttccgtactc
     8581 aatttcccgg tattgcagga caatccgtac cgtatcgatc tggaagaaac cgcgaacctg
     8641 ctggatcgct ttgatccggg actgatcata ctcggcaaga gcatgattct gcatccggaa
     8701 ccggttgcgg acattcgtaa aatgctggac acaaagtcta ccaggccggt tatcatgtac
     8761 gacatggcac atgtgctggg cttgatcgga ccgcatttcc aaaatccatt tgcagaaggt
     8821 gcggacatca tcacgggctc cacgcacaag actttttacg gttcgcagcg aggtgtaatt
     8881 ggggctgcct atgaagaggg tgcaccggag tttgagttgt ggaaagcgat agagcggcgt
     8941 gcgtttcccg gagcagtcag caatcatcac ctgggaaccc ttctgggact gttgatggct
     9001 acactggaaa tgaatgcctt caaggaaacc taccaaccac aggttgttgc taatgccaag
     9061 gcctttgcta tagctttggc ggatgaaggt cttgaggtac agggagatcc ggaagttggt
     9121 tacaccgaaa cccatcaggt cgtggtggtt gtagggtatg cactgggctg tgcggtggcc
     9181 caggaattgg aggaaagcaa cattatcgtc aattttcagg ctattccatc agacgagagc
     9241 ttcacatcca gttcgggact tcgcatgggt gtcgcggaaa tgactcgttt cggaatgaag
     9301 gaggatgatt tcgtggagtt cgcagcgatt ttcaacgagg cagtccatgg aaggaatgtc
     9361 ggtgacgagg ttgcccgctt tcgtgagcgt ttccagactt tgcacttctg tttcgatgca
     9421 gattatcccg aatacctgaa caatttgtcc gggcttaact tagtataacc ggaatgttgt
     9481 cttcttttaa gaatcagt
//
LOCUS       DTVD01000087            9497 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1897_length_9497_cov_272.926605, whole genome
            shotgun sequence.
ACCESSION   DTVD01000087 DTVD01000000
VERSION     DTVD01000087.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_9497_cov_272.926605"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="EYO68_03075"
     CDS             complement(<1..798)
                     /locus_tag="EYO68_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIC06818.1"
                     /translation="MLEKLAEINERFENLTHELGQPDVTQDQERYRKLSQEHSGLQEI
                     VECYHRYLEIQQELDDNRELADAANEDQEIREMASQEIPELGRQLEELEKQIQLLLLP
                     KDPDDSRNIIIEIRAGTGGDEAALFARDLFQMYAHYVEARKWKLKLLNESKNDLGGFK
                     EITFSIEGKDVFSRLKFEGGVHRVQRVPKTETQGRIHTSAATVAVLPDVDDVEIEINP
                     GDLRIDVYRSSGPGGQSVNTTDSAVRITHIPTGLVVICQNEKSQLKNK"
     gene            complement(805..1011)
                     /gene="rpmE"
                     /locus_tag="EYO68_03080"
     CDS             complement(805..1011)
                     /gene="rpmE"
                     /locus_tag="EYO68_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIC06819.1"
                     /translation="MKLNIHPEYKETSFRCACGATWETQSTKGGETTVEICSNCHPFF
                     TGSAQNFVDSAGRIEKFKRRYNRA"
     gene            complement(1093..2487)
                     /locus_tag="EYO68_03085"
     CDS             complement(1093..2487)
                     /locus_tag="EYO68_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIC06820.1"
                     /translation="MSKSRPENGDSNNSKTNSDNSSKPNTLNPENGDSNNSKTNSDNS
                     SKPNTLNLIELKKKDINALIKIAREYDIENANSMRGQELLFALLQAQTKRKGIIYGAG
                     VLEALPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFNLRTGDTVAGQIRPPKESERYY
                     ALLKVEEINFRDPNKAFEKILFDNLTPLHPNEHLNLERKDNDLTTRVIDLVSPIGKGQ
                     RGVIVAPPRTGKTMILQSIANSITENHPEVDLIVLLIDERPEEVTDMRRSVDGEVVAS
                     TFDEPATRHVQVSEMVLEKAKRLVEHQRDVVILLDSITRLARAYNTVIPPSGKILSGG
                     VDSNALFKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMELVLD
                     RKPVEKRIFPAIDLSKSGTRKEELLVEKADLDRIWVLRKVLSQLNVVEALEFLIDKME
                     KFKENVNFLNMMDK"
     gene            complement(2968..4746)
                     /gene="lpdA"
                     /locus_tag="EYO68_03090"
     CDS             complement(2968..4746)
                     /gene="lpdA"
                     /locus_tag="EYO68_03090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIC06821.1"
                     /translation="MSLIEVKVPDIGDFEDVEIIEVICNVDDKVALEDPLISVESDKA
                     TMDVPSPVAGIIREIKVKAGDKVSEGSLILKLETKEYAKVKTQKSEKIPPSQNSKPDS
                     QSKTLKTAKSFSGKADMTCDVLVLGSGPGGYTAAFRAADLGQEVILVERYNRIGGVCL
                     NVGCIPSKTLLHAAKVIDEAESMSAHGVSFGKPKINIDQLREWKDGIVNQLTGGLKSM
                     AKQRKVQIITGMGEFMDPNHLCVTDADANKNTIHFENAIIAAGSLPVKLPFLPEDSRI
                     IDSTGALELTEIPEHLLVIGGGIIGLEMATVYHALGSKITIVEMLDGLMAGADRDLVR
                     PFQKRISKQYENIWLETRVTKVEALKKGMKVFFEGKNAPGKPQIFDCILSSVGRTPNG
                     FRIGAEKAGVAVDEVGFIATDSQMRTNVSHIFAIGDIVGQPMLAHKATHEAKVAAEVI
                     SGMKRHFDARTIPSVAYTDPEVAWAGITEEEAKKQGIAYGKGNFPWAASGRSLSLGRS
                     EGMTKVLFEEDTNRVIGVGIVGPNAGDLIAEGALAIEMGCDAEDLSSTIHPHPTLSET
                     VAFAAEAFEGTITDLYLPKKKKNSTS"
     gene            complement(4743..6068)
                     /gene="aceF"
                     /locus_tag="EYO68_03095"
     CDS             complement(4743..6068)
                     /gene="aceF"
                     /locus_tag="EYO68_03095"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIC06822.1"
                     /translation="MSSIKDILVPDIGEFDAVEVIEVLVKSGDAVSVEDPLLTMESDK
                     ASMDVPSPFSGTVKTVKIKAGDKVSQNDLILTLVVKELETAVTETSAETENSEATAPE
                     TKDIVSTRESESTSSGTVSKEPARRPPPIVMSVNQKSFLRAHASPSIRKFARELGVDL
                     SQVHGSGRKERITKEDVQEFVKQALGGTASGTAAAGSGIPAMPEIDFSKFGPVEIKPL
                     SRIQKKAAVNLHRGWLNLPIVTHHDEADITELEYFRKSLKDEAAKQGVKVTPLVFLLK
                     ACAVAIRKHPNFNSSLTADKENLVLKKYLHIGVAVDTPDGLVVPVIRDVEQKGLLELA
                     KELGEVSEKARAKKLKTNDIQGGCFSISSLGGIGGTAFTPLVNAPEVAILGVTRSRMM
                     PVWNGKEFLPRLMLPMDLTYDHRVIDGAQAARFMVDLCEILSDMRRMSL"
     gene            complement(6080..8740)
                     /gene="aceE"
                     /locus_tag="EYO68_03100"
     CDS             complement(6080..8740)
                     /gene="aceE"
                     /locus_tag="EYO68_03100"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIC06823.1"
                     /translation="MTPKKVNDLDPQETQEWVEAMEAVVERDGFERAQFLLRELADHA
                     VLSGAGSPYSANTPYLNTIPPELEVRSKGDQELEHKIRSIIRWNAAVMVMRANRDSSD
                     LGGHIASFASSATLYDVGFNHFWQAAGKDHGGDLVFFQGHSAPGIYARAFVEGRLTEE
                     QMDNFRRESDGKGIPSYPHPWLMKDFWQFPTVSMGLGPIMAIYQARFLKYLHNRGLAD
                     TTGRKVWAFLGDGETDEPQSLGAIGLASRENLDNLIFVINCNLQGLDGPVRGNGKIIQ
                     ELEGDFRGAGWNVIKVIWGSYWDPLLAKDTEGFLRKRMDEAIDGEYQAFKAKGGAYTR
                     AHFFGKYPELLEMVSNMSDEDIWRLNRGGHDPHKIYAAYHSAVSHTGQPTVILAKTVK
                     GYGMGGSGEAINITHSQKKMKMDDLKSFRDRFQVPISDKEVEQMSFYRPPDDAPELEY
                     LKQCRTALGGHLPARRTKGDSLDIPALTIFERLLQETGEREISTTQALVQAMTLLCKD
                     KNIGQRVVPIVPDEARTFGMEGMFRQIGIYAHEGQKYEPVDRDQLMYYKENQSGQLLE
                     EGITEDGAMSSWIASATAYSNSGMQMIPFYIFYSMFGFQRIGDLVWAAGDMQARGFLI
                     GATSGRTTLNGEGLQHEDGHSQIMAGNVPNCVSYDPTFSHELMVIMQNGMERMFKKQE
                     NVFYYITTMNENYSHPGLSKGQEKNIVKGMYLLQKGKKGETRVQLLGSGSILREAIAA
                     ADLLSADFKVEADIWSCTSFNQLKRDGDDVSRWNMLHPDKKPKMSHVEKCLLDTDGPV
                     VAATDYVKLFADQIRAYVPRSYSVLGTDGFGRSDSRQALRKHFEVDRYYIAVASLNAL
                     MEDGSVSFKTVNAAIKKYGIDPNKNNPLMS"
     gene            complement(9127..9474)
                     /locus_tag="EYO68_03105"
     CDS             complement(9127..9474)
                     /locus_tag="EYO68_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06824.1"
                     /translation="MSKTRARITGPVKPGILYSEESSRGVLEMTKTRARITGPVKPDI
                     IYSKKVSREAMEMTLKRSQKAVSAGKTVSAGKGIVYSAKTLRKMSKRPTIVYAPFKSE
                     KAGEKKKTDVEKN"
BASE COUNT         2496 a         2211 c         1803 g         2987 t
ORIGIN      
        1 tttatttttt aactgggatt tttcattttg acaaatgaca accagtccgg taggaatatg
       61 ggtaatccga actgcagaat ccgtggtgtt aacgctttgt ccacccggcc cagatgatcg
      121 ataaacatca atccgtaaat ctccgggatt gatctcaatc tcaacatcat ctacgtccgg
      181 caacaccgct acagttgcag cagaagtgtg gatacgtccc tgcgtttcag ttttgggaac
      241 ccgctgtaca cgatgtacac caccctcaaa tttcaagcgg ctgaatacat ctttgccctc
      301 tattgaaaaa gtgatttcct tgaaaccacc aagatcattt ttgctttcgt tgagcagttt
      361 caatttccat ttacgtgctt caacataatg tgcgtacatt tgaaaaaggt cccgggcaaa
      421 gagagctgct tcatcgccac ctgtgcctgc acgaatttca attatgatat tacgggaatc
      481 gtctggatct tttggaagga gtaaaagttg gatttgcttt tccaactctt ccaactgtcg
      541 gccgagctcc ggaatttcct ggctggccat ttcacggatt tcctgatctt catttgcagc
      601 gtctgctaat tcgcggttat catcgagttc ttgctggatt tcaagatatc ggtgataaca
      661 ctcaacaatt tcctgcagac ctgaatgttc ctgactcaac ttccggtaac gctcctgatc
      721 ctgagtcacg tcgggttgtc cgagttcgtg cgtcagattt tcgaagcgtt catttatttc
      781 cgcaagtttt tccagcatct cgggtcaggc ccggttgtaa cgacgtttaa acttttcaat
      841 tcgacctgca ctatcaacaa aattttgtgc tgatccagta aaaaacggat gacagtttga
      901 acaaatttct actgttgttt ctccgccctt tgtagactgt gtctcccaag tcgctccaca
      961 ggcacagcgg aatgaggttt ctttatactc cgggtgaata tttaatttca tttttcgttt
     1021 ttccgattta agttttttaa agtttgaggt ttaagggaaa atgtatttgc tgtctgcaat
     1081 ttactttttg ttttatttat ccatcatatt gagaaagttc acattctctt tgaatttttc
     1141 cattttatca atgagaaatt ccagagcctc aactacattc aattgagaga gaactttacg
     1201 taagacccaa atacggtcaa ggtctgcttt ttcaacaagc agttcctctt tgcgtgttcc
     1261 ggatttgctg agatcgattg caggaaagat ccgtttttca acaggtttcc ggtcaagtac
     1321 aagttccata ttacctgtac ctttgaattc ctcaaaaatc acttcgtcca tcctgctgcc
     1381 agtatcaatc agcgcggttg caataatggt caaagatccg ccttcttcaa tgtttctggc
     1441 tgcaccaaaa aatcttttag gtttaaaaag tgcgttggaa tcaactcctc cggaaagaat
     1501 ttttccgctt ggaggaatga ctgtattgta ggcccgtgcc agcctcgtta ttgagtccag
     1561 caaaattaca acgtcacgct gatgttcaac aagtcgtttt gctttttcca aaaccatttc
     1621 agaaacctgt acgtgccgcg ttgccggttc atcaaaagtt gaggcaacca cttctccatc
     1681 tacggaacga cgcatatctg tcacttcctc aggtcgttcg tcaattaaca aaacaatcaa
     1741 atccacttca ggatgatttt cagtgatact gtttgctatt gactgcagaa tcattgtttt
     1801 accggttctt ggcggagcaa caatcactcc gcgctggcct ttcccaattg gcgaaacaag
     1861 gtcaatgacc cttgtggtca aatcattatc ttttcgttca agattgaggt gttcgtttgg
     1921 atggagagga gtgaggttgt caaaaagaat tttttcgaat gctttatttg ggtcacggaa
     1981 attaatttct tcgaccttaa gcagagcgta ataacgttcg ctctcttttg gcggcctgat
     2041 ttgccctgca actgtatcgc ctgtccgtaa attaaaacgc cttatctgtg aaggtgaaac
     2101 ataaatatcg tctggtccag ggaggtagtt gtaatccggt gctctcagga atccgaaccc
     2161 gtccggaagg gcttccaaaa ctcctgctcc gtaaattatc cctttgcgtt tcgtctgggc
     2221 ctgcaaaagc gcgaagagca gttcctgacc cctcatgctg tttgcatttt cgatatcata
     2281 ttcacgtgca atcttaatca gtgcattgat atcttttttc ttcaactcaa ttaaattgag
     2341 tgtatttggc ttggatgaat tatctgaatt tgtttttgaa ttattgctgt cgccattttc
     2401 aggattgagt gtatttggct tggatgaatt atctgaattt gtttttgaat tattgctgtc
     2461 gccattttca ggcctgcttt tagacatgtg aggtttagtc ggaggttaat caatgatagg
     2521 atcccttaat cttagagacc attttctgat gtgtttagtt aaatggaagg atgataaatt
     2581 tcaacgtatt tgcaacaact tcaattacaa aaagtggtta tagtgattaa taaaaacaac
     2641 gtaggtaagg tattgtctac tgtggaactg cttgatctag aattgtgcta ttaataaata
     2701 gggtactcca acttcgccaa tattcaatac attgtcaagt gtttttataa aaaaaaatat
     2761 tttttctgaa tcagttattt ttccagtgag acattaattc tcgtaatata ctgatattat
     2821 tgactacgac atgtaatctc tgccgaaggg agagatctga taaagtgcca atatcgtata
     2881 agatccctca cacatgttcg ggatgacacg ataaaactcg tgactgtcga gttctttttt
     2941 aaaccacaaa tattttgctg ccttgcacta tgatgtgctg ttttttttct tttttggaag
     3001 gtagagatca gtgattgttc cttcaaaagc ctctgccgca aaggccactg tttccgaaag
     3061 tgtcggatgc ggatgaatcg ttgaacttag atcctctgca tcacatccca tctcaatcgc
     3121 cagagcacct tctgcaatca aatctccagc atttggcccg acaatcccta ctccaatgac
     3181 tctgtttgtg tcttcttcaa aaaggacttt agtcatgcct tcactgcgtc ccaaagataa
     3241 actgcgtcca cttgcagccc aggggaaatt tccttttccg taagcaatgc cctgcttttt
     3301 tgcttcttct tcagtaatac ctgcccaggc aacttccgga tcagtatatg ctacagacgg
     3361 aatcgtccgg gcatcaaaat gccttttcat tcctgaaata acttctgcag caacttttgc
     3421 ttcgtgtgtc gctttatgtg ccagcatcgg ctggcctaca atatcaccaa tcgcaaaaat
     3481 atgagaaacg tttgtacgca tttgcgaatc agtcgcaata aatccaacct catcaactgc
     3541 taccccagct ttttctgcac caatccggaa tccatttggg gtacgtccta ctgaactgag
     3601 aatgcagtca aatatctgag gcttgcccgg tgcattttta ccttcaaaaa aaactttcat
     3661 tccttttttg agtgcttcta cctttgttac ccgtgtttca agccagatat tttcatactg
     3721 tttggatatt cttttttgaa aagggcgaac taaatcccgg tctgcacctg ccatcaagcc
     3781 atcaagcatt tcaacaatgg ttatttttga acctagagca tgataaacag tagccatttc
     3841 cagacctata atacctcctc caattaccag cagatgctct ggaatttcag taagctccaa
     3901 tgcaccggtt gaatctataa tacgtgaatc ttccggaaga aaggggagct tcaccggcag
     3961 agaacctgct gcaataatcg cattttcaaa atgaattgta tttttatttg catcagcgtc
     4021 agtaacgcaa agatggttag ggtccatgaa ctcacccatg ccggtaatga tttgcacttt
     4081 gcgctgtttg gccatgctct tcagacctcc ggttaactgg tttacaattc cgtctttcca
     4141 ctcacgcaac tggtcaatat taatttttgg ttttccaaaa gatactccgt gtgcactcat
     4201 cgattcagct tcgtcaatga cttttgctgc atgtaacagt gtctttgacg gaatacagcc
     4261 aacattcaaa cacacaccac caatacgatt gtagcgttcc accaaaatta cttcctgacc
     4321 taaatccgct gcacgaaaag ctgcggtgta tcctccaggt ccggaaccca gcacgagcac
     4381 atcgcatgtc atatctgctt ttccggaaaa ggattttgcg gttttgagcg tcttcgattg
     4441 agaatctggt ttcgaatttt gggatggcgg aattttttca gatttttggg ttttaacttt
     4501 tgcgtattcc tttgtctcca attttaaaat caggctgccc tcagaaactt tatctcctgc
     4561 tttaactttt atttcacgga ttattcccgc aaccggtgag ggaacatcca tggttgcctt
     4621 gtcgctttcc acagaaatca gcggatcctc cagtgcaacc ttatcgtcta cattgcaaat
     4681 tacttcaata atttcaacat cttcaaaatc gccaatatcc ggaaccttta cttcaatgag
     4741 actcataacg acattctccg catatctgac aaaatttcgc acaagtctac cataaatcga
     4801 gccgcctgtg ctccatcaat cacacggtga tcataagtca aatccattgg cagcatcagg
     4861 cgcggcaaaa attctttacc gttccagaca ggcatcatac gcgaacgcgt tacaccgaga
     4921 attgcaactt ccggagcatt caccaaaggt gtaaaagctg ttccgccaat tccgcctaaa
     4981 ctggaaatgc tgaaacaacc gccctgtatg tcgttggttt ttagtttttt tgctcgagct
     5041 ttttcactaa cttcacccag ttctttggcc agttccagca aacctttctg ttccacatcc
     5101 cttatcacag gaaccaccag gccgtccgga gtatccactg ccactccaat gtgaagatat
     5161 ttcttcaaca ccaaattttc cttgtctgca gtcaaggatg agttgaaatt tggatgcttc
     5221 cgaattgcca cagcacatgc tttgagtaaa aaaacaagag gagttacctt cacaccctgc
     5281 tttgccgctt cgtctttgag cgattttcga aaatattcga gttcagttat atcagcctca
     5341 tcatgatgcg tcacgattgg taagttcagc catcctcgat gcaaatttac agcagctttt
     5401 ttctgtattc tggaaagcgg tttaatttct acaggaccaa atttactgaa atctatttca
     5461 ggcattgccg gaattcctga tccggcagca gcagttccgg aagctgttcc tcccaaagcc
     5521 tgcttgacaa attcctgaac atcttctttt gtgatacgtt ctttgcggcc ggaaccatgg
     5581 acctgactca ggtcaacacc taattcgcgg gcaaatttac ggatcgaagg actggcatgt
     5641 gcacgcaaaa atgatttctg gttaaccgac atcactattg gcggtggacg ccgtgccggt
     5701 tctttggaaa cagttccaga acttgttgat tcagattctc ttgtggaaac aatatcctta
     5761 gtttccggtg cagttgcttc agaattttct gtttcagcag acgtttccgt aactgcagtt
     5821 tcaagttctt ttaccactaa agtaaggatc aggtcgtttt gtgaaacttt gtctccagct
     5881 tttattttta cagtctttac tgtaccgcta aacggagaag gaacatccat tgaggcctta
     5941 tcactttcca ttgtgagcag cggatcttca acagaaacgg cgtctcccga tttaaccaga
     6001 acttcaataa cttctactgc atcgaattca cctatgtcag gaacaagtat gtctttgata
     6061 ctactcatgg cgtttctcct caggacatca atggattatt tttgttgggg tcaattccgt
     6121 attttttgat ggcagcattt actgttttga atgaaacact cccatcttcc ataagagcat
     6181 ttaacgatgc cactgcaatg taataacggt ctacttcaaa atgctttctc aaggcttgac
     6241 gtgaatcgct tctgccaaat ccatcagttc caagcacaga ataagatcga ggtacatatg
     6301 cacgtatctg gtcggcaaaa agtttgacat aatctgttgc cgcaactaca ggaccgtctg
     6361 tgtctaagag acatttttca acatgactca tttttggttt tttatctgga tgcagcatgt
     6421 tccagcgtga aacatcatcg ccgtcacgct tcagttgatt gaaactggta cagctccaga
     6481 tgtccgcctc caccttaaaa tctgcagaca gcaaatctgc cgcagcaatc gcttcccgta
     6541 aaattgatcc ggaacccaac aattgtactc tcgtttcccc ctttttacct ttttgcagca
     6601 aatacatacc tttcacaatg tttttttcct gacctttact taagcccgga tgcgaataat
     6661 tttcattcat tgtggttatg taataaaaaa cattctcctg ttttttaaac atgcgttcca
     6721 ttccattctg cataatcacc atcaattcat gagaaaaagt cggatcataa gaaacacagt
     6781 tgggcacatt tccagccatg atttgactgt gtccatcttc atgctgcaat ccctcaccat
     6841 tgagtgtggt tcgtccagaa gtagcgccaa ttaaaaatcc ccgtgcctgc atatctccag
     6901 cagcccaaac taaatctcca atccgctgaa atccaaacat cgagtaaaaa atataaaatg
     6961 gaatcatctg cattccggaa ttgctgtagg ccgtcgcaga agcaatccag gaagacatgg
     7021 cgccgtcttc agtaatgccc tcctccaata attgaccgct ctgattctcc ttgtaataca
     7081 ttaattgatc acgatcgaca ggttcgtatt tttgtccttc gtgcgcataa attccgattt
     7141 ggcgaaacat tccctccatg ccaaatgttc ttgcttcatc aggcactatg ggaacaaccc
     7201 gctggccgat gtttttatct ttgcacaata acgtcattgc ctgcacaagt gcctgagttg
     7261 tggatatttc ccgttctcca gtttcctgta ataatcgctc gaaaattgtt aatgcgggaa
     7321 tgtcaagaga atctcctttt gttctacggg cgggcaggtg gccaccaaga gcagtgcggc
     7381 actgtttaag atattccaat tcaggagcat catcaggagg cctgtaaaac gacatttgtt
     7441 ccacttcttt atcagaaatt ggaacctgaa aacgatccct aaaacttttc agatcgtcca
     7501 ttttcatttt cttttgtgaa tgagtgatgt tgattgcctc accagatcct cccatcccgt
     7561 aaccttttac cgtttttgcg agaattacag ttggctgtcc cgtatgactt actgcagaat
     7621 ggtatgctgc gtatattttg tgaggatcat gcccaccacg gttcagtctc caaatgtcct
     7681 catcagacat attagaaacc atctccagca attcagggta tttaccaaaa aagtgagctc
     7741 gggtatatgc tccacctttt gctttaaaag cctgatattc tccgtcaatt gcttcatcca
     7801 tgcgtttacg cagaaaacct tcagtgtctt ttgccagcaa aggatcccag tatgaacccc
     7861 agataacttt tatcacattc caacctgcac cacggaaatc accttcaagt tcctgtatta
     7921 tttttccgtt tccacgtact ggaccatcta gtccttgcaa attgcagtta ataacaaaaa
     7981 taagattgtc caaattttca cgggaggcca ggccgattgc tcccagagat tgcggttcat
     8041 ctgtttcacc gtcccccagg aaagcccata ctttgcgtcc ggtagtgtct gctaaaccgc
     8101 ggttgtgaag gtatttgagg aaacgtgcct ggtaaattgc catgattggc cccagtccca
     8161 tggaaacagt tggaaactgc caaaagtctt tcatcaacca tggatgagga tatgaaggaa
     8221 tacctttacc atcggattca cgacgaaaat tatccatttg ctcttccgtc agtctgccct
     8281 ccacaaaggc ccgtgcatat attcctggtg ctgaatgacc ctgaaaaaat actaaatcac
     8341 caccatgatc tttacctgct gcctgccaaa aatggttaaa accgacatca tacagtgtag
     8401 ccgaagaagc aaaactggcg atgtgccctc ctaagtcaga tgaatcccga tttgcacgca
     8461 tgaccattac cgctgcattc caacggataa ttgagcgaat cttgtgctcc agttcctgat
     8521 ctcctttaga acgtacttca agttccggtg gaattgtatt cagatacggt gtattagcag
     8581 aataaggact gcctgctcca gacaaaaccg catggtctgc taattctcgt aagaggaatt
     8641 gtgccctttc aaaaccgtct ctttcaacaa ctgcttccat agcctctacc cattcctggg
     8701 tttcctgcgg atctaaatcg tttacttttt ttggagtcat tgttttacct cagtcaatga
     8761 tgtgtttgga tcctacaatt tgataatacc ttttattagc aaaaaagcca tatttgtttc
     8821 ttatattgat tattagtgct tgaaagaaaa aaataatatg ttgtaataac agttaattat
     8881 ttttgatttc agcaatgagg attttacaaa aagctttcag cagtcagcta acagttcttt
     8941 ggcctgattt ttcattctgt tagcagaaaa cttaaaattg acaactaaat ctctggttat
     9001 tcaaaaactg ctgaagccct gataaaaact ggggtagata gattttcgtt cagacactat
     9061 ttaagttttc accacaatta aattgacggg gaaagttcct gggaaatttc aaggggttga
     9121 actgacttaa tttttttcga catcggtttt tttcttctca ccggcttttt cacttttaaa
     9181 tggagcataa acaatggtcg gccttttact catttttctc aatgttttag cagagtagac
     9241 aatgccttta cctgcagata cagtttttcc tgcagataca gctttttggg agcgcttcaa
     9301 tgtcatttcc attgcctcac gacttacctt cttagagtag ataatatccg gcttaacagg
     9361 tccagttatg cgcgcacgtg ttttagtcat ctccaatact ccacgacttg attcttcaga
     9421 ataaagaatg cccggcttga ctggtccagt tatgcgtgct cgtgttttag acatctgtag
     9481 tgccttgcgg cttattt
//
LOCUS       DTVD01000088            2371 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_19173_length_2371_cov_476.277634, whole genome
            shotgun sequence.
ACCESSION   DTVD01000088 DTVD01000000
VERSION     DTVD01000088.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19173_length_2371_cov_476.277634"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(79..1368)
                     /locus_tag="EYO68_03110"
     CDS             complement(79..1368)
                     /locus_tag="EYO68_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC06825.1"
                     /translation="MKQLHPEYGFNTLAVHSGTAPDPTTGARSFPIYQTTAYVFDDAD
                     HAASLFNLQEPGFIYSRLTNPTVAALEEKLATLEGGRGGTVTSSGHAAETLALFPLMA
                     PGMEVIVADKLYGGTINLMGKSYRKFGWNAVFVDTDNPEAFRKAVTEKTRAIFIENLA
                     NPGGVVVDLEAIAKVADEAGVPLVVDNTMATPFLCQPFEWGADLVVHSTTKFLSGQGN
                     AVGGVVIDSGKFPWLGNSKFPSLSTPSPEYNGLTFAETFGDLAYTMYSHAISLRDLGC
                     TQSPMNAWITLNGIETLPLRMERHCSNGLAVAKFLEKQANVEWVSHAGLESSPYFELG
                     KKYLPEGTGSVFTFGVKGGYESGLKMLESVELFSHVANIGDTRSLILHPASTTHRQLT
                     EEQRIAAGAGPEVIRLSIGIECVEDIISDLDQALNCL"
     gene            complement(1368..1865)
                     /locus_tag="EYO68_03115"
     CDS             complement(1368..1865)
                     /locus_tag="EYO68_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIC06826.1"
                     /translation="MPGPASVDHNSYPDSYIREILRNVRSIAMVGASATWNRPSNFAM
                     KYLLKKGFKVIPVNPNAAVKKICGETVFPDLSSLSGTVDMVDIFRPAEDAPELVEEAI
                     KIGAKVFWMQLEIRHAEAAKRAEEAGLQVVMDRCPKIEYARLTGELGWNGINTGVISN
                     RRKRL"
     gene            complement(2123..2305)
                     /locus_tag="EYO68_03120"
     CDS             complement(2123..2305)
                     /locus_tag="EYO68_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06827.1"
                     /translation="MKSWKVLLAIALAIFAISWFIVSPETKTFSVLSQRVDTSKSAAI
                     TDKWDGKGKFIDMIWE"
BASE COUNT          686 a          556 c          485 g          644 t
ORIGIN      
        1 ctggcaattt atgttggcta cctgtatcat ctgtggcctg cttaaactgc acttaaaagc
       61 ctcatgtttt cagatttttc ataagcagtt caaagcctga tccaaatctg aaatgatatc
      121 ttcaacgcac tcaattccaa tggaaagacg aatcacttcc ggtcctgcac ctgctgcgat
      181 acgctgttcc tctgtgagtt gtcgatgagt ggtggaggct ggatggagaa tcagtgagcg
      241 ggtgtcacca atgttggcca catgactgaa caactcaacg gattccagca tttttaagcc
      301 gctttcataa ccccccttga caccaaaagt aaaaacagaa cctgtacctt caggcaaata
      361 ttttttccca agctcaaaat aaggactgga ttcgagacca gcgtgagaaa cccattctac
      421 gttagcctgt ttttcaagaa attttgctac agccagaccg ttggaacagt gccgttccat
      481 gcgcaaaggc aaggtttcaa tgccgttgag agtaatccag gcattcattg gagattgcgt
      541 acagccaaga tcacgcaaac tgatggcgtg actgtacatg gtgtatgcaa ggtctccaaa
      601 agtttctgca aatgtgagcc cgttatattc cggtgagggc gtactcaaac ttggaaattt
      661 ggaatttccc aaccagggga attttcctga atcaataacc actcctccga ccgcatttcc
      721 ctgtccggac aggaattttg ttgtggaatg cactaccaaa tccgctcccc attcaaatgg
      781 ttgacacaaa aaaggagtcg ccatcgtgtt gtctacaacc agaggcactc ctgcttcatc
      841 agcaacttta gcaattgctt ccaggtcaac aacaactcca ccgggattgg caaggttttc
      901 aataaaaatt gcccgagttt tttcagtaac cgccttgcgg aaagcttccg gattatcggt
      961 gtcaacaaaa actgcattcc agccaaattt ccggtaactt tttcccatta aattgatcgt
     1021 tccgccgtac aatttgtctg ccactatcac ctccattcct ggagccatca acggaaacaa
     1081 agccagagtt tccgcagcat gtccggaaga ggttactgtt ccaccgcggc cgccttcaag
     1141 cgtggccagc ttttcttcta aagctgctac agttgggtta gtcaaacgag aataaataaa
     1201 gcctggttcc tgcaagttaa acagtgatgc agcatggtct gcgtcatcaa atacataggc
     1261 ggtagtttga taaattggaa aagagcgcgc acctgtcgtt ggatctggag ccgttccaga
     1321 atgaactgca agtgtattaa aaccatattc tggatgcagt tgtttcatta aagcctcttc
     1381 ctgcgattac tgattacacc tgtattgatt ccgttccagc ccagctcacc agtcaagcgg
     1441 gcatattcaa ttttaggaca gcgatccatc accacctgta gtcctgcttc ttcagcacgt
     1501 tttgctgctt cagcatgacg gatttccaac tgcatccaga aaacttttgc tccaatttta
     1561 atggcctctt ctaccagttc tggagcatct tctgccgggc ggaaaatatc aaccatgtcc
     1621 acagttccag agagtgaaga taaatccgga aacaccgttt ctccacaaat tttcttgaca
     1681 gctgcattag ggttgaccgg gataacctta aatccttttt taagcaaata tttcatggca
     1741 aaattgctgg gacggttcca ggttgcactg gctccaacca ttgcaatgct gcgtacattt
     1801 cttagtattt cacgaatata agaatccgga taggagttgt gatcaacgga tgcaggaccg
     1861 ggcatggaag aaaatcagga ttagcggtga ttagtcttgg atggatacct tagcaaaata
     1921 tatcacgcca cgcaaataaa ttcaacgttg tgcatcaggc tacaggttaa cataaggagg
     1981 aaaaaattag gaagattcat atttcatggg tcatcaagcg caaaggctaa agcactttca
     2041 accagaaact atcagttatt aacaggttcc tgaattcatc tgtgataata cttgacctga
     2101 aaccgtatgg cggaaccgtt tttcattccc aaatcatatc aataaattta cctttaccat
     2161 cccatttatc agtaattgcc gcagatttgc tggtatccac tctctgagac agtacactaa
     2221 aagtttttgt ttcaggagaa acaataaacc aggaaatggc aaatattgct agagcaattg
     2281 ccaataagac tttccaactt ttcatactac ctccttcaca gaagagcagt ggacaaaatt
     2341 atttgaaaac aaagtggtaa aacaggaaag c
//
LOCUS       DTVD01000089           29803 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_191_length_29803_cov_492.433743, whole genome
            shotgun sequence.
ACCESSION   DTVD01000089 DTVD01000000
VERSION     DTVD01000089.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 29803)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29803)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29803
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_29803_cov_492.433743"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            192..722
                     /locus_tag="EYO68_03125"
     CDS             192..722
                     /locus_tag="EYO68_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HIC06828.1"
                     /translation="MKNNIYAKWIFGAIYSDSCWLEQAKEQLEKQNWKIQHQSPEFTF
                     DQTDYYASEMGTRLKRRFLSVSGIYSMESSADWKLRMVKIENRFRRNGKRRINLDPGF
                     LDLHRVVLLSGKEGPQKIYLRDGVWADLVLLKNKGGYRDLAWTFPDLRDGRYNDFFLQ
                     VRAEFKEEKSLSSNIS"
     gene            784..1758
                     /gene="miaA"
                     /locus_tag="EYO68_03130"
     CDS             784..1758
                     /gene="miaA"
                     /locus_tag="EYO68_03130"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIC06829.1"
                     /translation="MVHGKNYLQNHLVVYKYSMEIFPKIVVVAGPTASGKSDLALRLA
                     QEFDGELICSDSMQVYRQMDIGTAKPTPDVQKIVPHHQLDLIDPDESYSAGKYARDAS
                     SIIEKVAARKRLPILVGGTGLYYRALMYGISKIPSIPEKHRKKVTGWHSEHGTYYCWK
                     ELQKLDPEGAVRLHPNDTARILRSLEVVLSTGTTLAEFQQDKPFAEAKYSFHAVALEW
                     ERDVLYERINQRTHIMLESGWITEVEKLLTRYSPELKPLQAIGYREVIQHLQNKLEWG
                     AMVEEIQKRTRQYAKRQMTWFRKENNIGWHKPDDIDEILDKIKVYLEK"
     gene            1837..2226
                     /locus_tag="EYO68_03135"
     CDS             1837..2226
                     /locus_tag="EYO68_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06830.1"
                     /translation="MKTYTVDHQNYHIFKTGIGAKKQFVHFQWGKFDFRMSFIILTNI
                     KQDNPEKTISAKNGSKYLIEKFEVLYQNEWFEFIKPTAHGMQLEETLWHRNGQDYYVE
                     FPKDLSSVALEICAEELELKVLQDVAA"
     gene            2319..3683
                     /gene="miaB"
                     /locus_tag="EYO68_03140"
     CDS             2319..3683
                     /gene="miaB"
                     /locus_tag="EYO68_03140"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIC06831.1"
                     /translation="MEEASQNTETVKKVFIQTYGCQMNEYDTEKMFEVLKHQNYFQTD
                     SREDADLIILNTCSIREKAENKVYSELGRLRKLKSVKPELKIGVGGCVAQQEGKAILR
                     REASVDFVFGTDNLFELPEMLKKVSRGQEIIQTKRHARQKVRNFIPEYAFQQTQHHGI
                     KAHLAITKGCNNHCSFCVVPFTRGIEVSREPENILAEAQRLTSAGTREICLLGQNVNS
                     YKANGVDFVELLHRLDELENLQRLRFISPHPKDFHEELADAFVTLPSLCEQMHLPLQS
                     GSDRILRRMRRWYTMETFYEKVAMLRDRLPEATLSTDLIVGFPGETEEEFEMTMEAVR
                     TIRFDLIYSFKYSTRPGTRAAEYPEHLAEDVKSKRLQILLETQGKIVQEKNEALVGSI
                     QEVLVQGKHYRKNSITGRTRGNHSVHINDSCAIAGDLLSVRITGALHNSLEAEIISKS
                     TVLS"
     gene            complement(3751..4014)
                     /locus_tag="EYO68_03145"
     CDS             complement(3751..4014)
                     /locus_tag="EYO68_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06832.1"
                     /translation="MLFPTKSLFLFIKYSIRRYFIKIIFVASTTTVLGCSAAVWKNHG
                     DSCIHISEKPPLRRVINYSDHELCGRKLSPRTAPNPLTRPQTK"
     gene            4178..5368
                     /locus_tag="EYO68_03150"
     CDS             4178..5368
                     /locus_tag="EYO68_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06833.1"
                     /translation="MIVNRIGVGVSKETDPDVAIAEVLHSAVKQAKLPKVNWVLTFFT
                     PAHFIHAGRLHELIREQTNCDCITGCSGMGVLSVLGEISSGPGLVLMAGYTPELRPLA
                     IAKYQELEHSAGVTQQFRETLENFGGEKPLFFFFPDVYQHQPHNFINMFNFLKNHPSV
                     FGAGSCNDGSQETSIQFGPDIVTLNGAGGLALDGVPEFSAGVTQSCATFEEPMFITET
                     KDDLILSLDGKPALEVFNEVANELGFSNLELAAQQLLLSFPLDPEQPVFTGEGSMARH
                     VTRIDAASQGISTSQIVHEGGVVSFAYRNNATAEQDLQAMLSRLKNKNTNTPTFGIYF
                     NCASRGEALYGRQNVDTQLIRETLGEFPLIGFFGGYELAQMPQGVQLYTYTGVLVLVY
                     LENK"
     gene            5510..6076
                     /locus_tag="EYO68_03155"
     CDS             5510..6076
                     /locus_tag="EYO68_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06834.1"
                     /translation="MKNLLCRSAKSAAKKRVLFSNYFFGIAIALLLSGCGYVIEGSNP
                     VLPNEAQTLALLPIQNQTFKAGLETDLVEQLSRLLRSNSSVKMLTSGQADLRLKITLL
                     KLKTSSSGLSKDQISTGVKAILQGEVILEDMRTGSKVWEEKMLQVKLSESGGNDSDTV
                     SSVSLSGSIRELIKQFAKKLYNRLFTTF"
     gene            6237..7859
                     /locus_tag="EYO68_03160"
     CDS             6237..7859
                     /locus_tag="EYO68_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIC06835.1"
                     /translation="MHDSFETGLPQNSANYTPLSPITFLKRTAFVHPHRTSVIHGKHR
                     WTWAETYIRCCRLASALSKRGIGKGDTVSVMAPNIPAIFEAHFGVLMTGAVLNTLNIR
                     LEAETLANIFEHAETKVLLTDREFSPQIKVALSKVKRDILVIDIDDPETESGEYLGML
                     EYEAFLAEGDPEFEAVLPEDDWQAVSLNYTSGTTGIPKGVVYHTRGAYLLATGNVLAW
                     EMPHRPVYLWTLPMFHCNGWCFPWTITMLGGTHVCLRKVTAKNIYNSVAEHHVTHLCG
                     APIVMNMISNAPKEEQRELPHRVEFMTAASPPPPTVIAQMEEAGFNVTHVYGLTEVYG
                     PAVVCEWQEAWTEKDKTTQAQLKGRQGVRYHVLEGLTVANPETLEPVPADGETMGEVL
                     MQGNIVMKGYFKNPEATAQAFAGGWFHSGDIGVLHPDGYIELKDRSKDIIISGGENIS
                     SVEIENVLYQHEDILEAAVVARPDKKWGEVPCAFVSLKTGCSLNEQEVRKFCRTKLSL
                     FKIPKYVVFCELPKTSTGKIRKSVLREQAKKS"
     gene            7961..9172
                     /locus_tag="EYO68_03165"
     CDS             7961..9172
                     /locus_tag="EYO68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIC06836.1"
                     /translation="MGMTKKKFIEDLNLNGLRVLTRVDFNVPLNKDLQITDNGRIEAA
                     LPTIRYIVEQGGKAILISHLGRPGGERVESLSLKPAAEELSLLLGQPVTFAKDCIGEE
                     VEALIEGMQNGEVLLLENLRFHKAETKNEPEFCGALGELGDVYVNDAFGTAHRAHAST
                     AGVTGFIPESAVGYLIRKELQFLGGAVMNPARPFTAILGGAKVSDKIKVIDKLLNKVQ
                     TLIIGGGMSCTFLKAQGYKIGASLLEEDLLDFAKELIQKAEEKGVKLLLPVDAVIADS
                     FDKSASTCSVSVEEGAPDGWLILDIGPKSAETFCDSVRKSGTVLWNGPMGVFEMEAFA
                     QGTFAIAQCLAEVTDKGVVTIIGGGDSAAAIQQFGLSERITHVSTGGGASLEFLEGKE
                     LPGVSAIQQSK"
     gene            9217..11499
                     /locus_tag="EYO68_03170"
     CDS             9217..11499
                     /locus_tag="EYO68_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06837.1"
                     /translation="MDKDTALQEDNDSTENQEENSEVTTEEKSTNVSFSFFLYRLIAY
                     ADARRSVASFVIILFLVTFSDILFENFLFVPYVELVESLSGVHPGGFAELDVGFAPEV
                     WQALLGMVLGTLILVISIASQSIPKLIDFYMRDIPSLLYIWFLIISGVHALIIKIYGE
                     IGLVREPSRIFNTHFLLTICTIIAFPYVFYILRYTKPTNIINRIYNNNMDQIRALTSS
                     RNRALAHIPKVVEYQQYTIFEALNQLDDILEFSSFKELKADIVHDMSVTLQNYIRLKR
                     DIAPGFFKVSPKVRTDISFKTMVGQFGEMERNQSFYEQKCFRLLGNVYIRLLEHGEFD
                     LSSMVAGEMANLGLTAIEEDNTELIDIIIIRFNTLLRFAIKHGVRNNEPRNLYNLGFY
                     YGNFIRYLVEHKKTDHVKRCFMYLRIYGVEIFKHGSNSPAMYFIVDVIATEMKKVLEQ
                     IYHDDWDKEIQNGMLSEILQVDSPPDFNKEDLARGVLINNGVRVLQFGLALFYQREGM
                     TDFVERIAKDVLDDLQALGEATFSQVIEMTSNRLLFSGPTFWEDTDRGNLNIYYTSDQ
                     DQIDGFKKRLYDLAETQLKTEMTQKYQLTEVELNLLWEMSRMTKEKEVFQISTNVKTF
                     ELILNDLEKIDEERLDALVTLREKLNFNSDNPKLIVTTSRQLAVGTELKISGKISGQT
                     KQLEFQAVAQLNTPNYLFVKVMDEGEVVKIEKLSSLTVKFRPLRQKMVYQFHATLQNT
                     GANSLLRIEHSNKVKIVEEL"
     gene            complement(11496..12230)
                     /locus_tag="EYO68_03175"
     CDS             complement(11496..12230)
                     /locus_tag="EYO68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIC06838.1"
                     /translation="MCGRFVMIESEEKVMQTFEIQQSEMMLEPRYNICPSQDIPVIVQ
                     QDGLRSLEMRQWGFIPFWAKEPTPMINARAETVSEKPFFRQAFRKQRCLIPATGFYEW
                     AKEEGQKQPYFISLKSEISDKGNSMMAFAGLWDSWTSPEGELRRTCTILTVAANSLMQ
                     KIHHRMPVILTPNNGLSWLDLSGTETAPEKLLIPLPAEKMEAWKVSRKVSVPTFDNPD
                     CLKKLDDSVTDEKNPEPPEAQASLFD"
     gene            complement(12233..13363)
                     /gene="tgt"
                     /locus_tag="EYO68_03180"
     CDS             complement(12233..13363)
                     /gene="tgt"
                     /locus_tag="EYO68_03180"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIC06839.1"
                     /translation="MIQFELEAKDHNSRAGILKTLHGTIHTPVFMPVGTLGTVKALTP
                     EEVSELGAEIILGNTYHLHLRPGDELVRKLGGLHHFMNWDGPILTDSGGFQIFSLAKL
                     LKLDQSGVVIRSHIDGSKITLTPEISIAIQQNLGSTIMMCLDQCLELPATRQEIERSI
                     ALTTKWAQRSKDASSQGSGVSGEQALFGIVQGGGELNLREQSLEQMVNIGFDGYAIGG
                     LSVGETKEEMYSVVHHIAVKMPAQKPRYLMGVGDPEDLLEGIEAGIDMFDCVMPTRNA
                     RNGSLFTSHGKISIKQNQYKEDPAPLDPDCACSTCKNYSRAYLRHLFKTNEILGMRLN
                     TYHNLFFYISLIKQARNAIKEKRFPNFKKTFLQKYRSGIEGV"
     gene            complement(13372..16149)
                     /locus_tag="EYO68_03185"
     CDS             complement(13372..16149)
                     /locus_tag="EYO68_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HIC06840.1"
                     /translation="MSAITFDPESSWQSSFTFNHVKCLIVCRGPIRLETMNIFKELGA
                     TYGMLLSEKDSIIFPQTLAPELRAIRNRRKQVHHVPDYMGATKEERVERIEQVIDICH
                     ENGYTHLFAGYGFMAEDAEFVERIENAEISFIGPNSKVIQQAGSKDEAKQLARSLNVS
                     VTPGEDRIDALTLLRKAGKAPDTYFPKIIKNHKLSLPENWQSEELIDQAVTILQASYA
                     KQIDLFTIEELQQETVRQVESIWLENPGKRIRLKHVGGGGGRGQRVVTSVEEIAEAVM
                     SVLIESKATGEGDNKNFLIELNIENTRHNEIQLLGNGKWCIELGGRDCSLQMHEQKLL
                     EVSLTEEMLEAAAREYESAGKTPQAKVLRSDAKMLSSMCKEAEEFGKALNLDSVSTFE
                     CIVEGEKHFFMEVNTRIQVEHRVTEMAYQLQFSNPDNPEDTFVVNSLVAAMLLINCYG
                     EKLYRPKRLPRFVSGVEARLNATNPALKPHAGGVLRAWSAATENEIRDDQGIGISNPD
                     TGLLQPYNLAGAYDSNVALSITHGISRRESFEKLTEILRCMEVRGHDLHLNVDFHYGL
                     LHWLLGNDPMLKPNTRFVSSYLALAGKLKNFCDQINLDLAWKIQRDQVQKNYGSDGLQ
                     IYDQKITLILRPLKKLLNKTHLLMGWLSFQKSKNLHSKLSTFQNPVQTLADLYHFLRL
                     EQHSGVPPSEQIWSNDQKLLETASAFYSELKTRFGKTNLSWQELMEILSAEKPPAKFK
                     LGKNLKLWEGISAAHKGHQMSMELLQLPSILAKKAGYYDFRGNNTLEVKIPKEFQDPE
                     SNAELIAKLAPPPSAKSNEILAWTGGTFYSRETPEAGEYVREGQHVEEGDVLGLLEVM
                     KMFNQIRAEFPGTIRKICIDAGTGKIVARSQVLFQIDPDIPPVSETEEELFKRQQEQT
                     ISLMQSIIPAN"
     gene            complement(16343..17566)
                     /gene="apgM"
                     /locus_tag="EYO68_03190"
     CDS             complement(16343..17566)
                     /gene="apgM"
                     /locus_tag="EYO68_03190"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIC06841.1"
                     /translation="MQRIPRLLLIFDGMGDRPIVELGDKTPLQAANLPVMDQLALEGQ
                     CGVADPVRSGTVATTVMGTLAILGYDPHRFSIARGLIEAIGCGMKIMPGDVAFRGNWA
                     TLDDEGMIVDRRAGRIREGTKELSSSLCGLKIDDVSLYVGSGTEHRVALVIRGSGLGD
                     CLSGSDPGDHFLSGKKPRHPEVLKKNDKKSLRTAKYLHLFELAARKILATHPVNLERK
                     SKRLLPANSILTREPGQVHAFPILKRPSGLGLSGICVTGDDTILGIAKVTGMDVCKTP
                     EMTANLDTDLNKKFEITAKLLKQYGVVVLHIKGCDIAAHNRDAEKKKDFLERIDTELG
                     RFLGKWPGKLRLCITADHTTWSKEGVHTDDPVPVLLHGRGIRADSIKEFNEIQALKGQ
                     LGRFRMYKLWEKFFA"
     gene            complement(17576..18745)
                     /locus_tag="EYO68_03195"
     CDS             complement(17576..18745)
                     /locus_tag="EYO68_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06842.1"
                     /translation="MMQLQVIRQDKSKHPFMRGMLAHKLMQRGLSFDQAYQISKDAKS
                     YFQEQTEVTSESLMQSVDELIVSRYGKELLRTLTSELFPSGKQICVFRRNATSPFSKG
                     LLTQSITAAGIKPEEAYKIAFDLEEDLIKKDILRISKNKLFEEVFSTIKKKYSPHLAG
                     LYKLASRIDELDRPVIIYLAGASGTGKSVMSTFLAGRLGINKITGTDTIREIMRLVFK
                     RDLLPSLHNSSSKAGVGMPKTLDKNARLISGFCLQAQQVSVGVKAVVDRAVKERTSMI
                     IEGVHLLPYMQQTLQEGTKRAYHIPITLSLMNDKHHKDRFFERGKGNELRKKEPYLRS
                     FENIRSIHEYCISESENNEIEVVDNEDFDETTNTLMQLIINTLQEQVKSTLTSKS"
     gene            complement(18742..19350)
                     /locus_tag="EYO68_03200"
     CDS             complement(18742..19350)
                     /locus_tag="EYO68_03200"
                     /inference="COORDINATES: protein motif:HMM:PF02391.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIC06843.1"
                     /translation="MADAPSRPDFGKYEHGVRQPCELERSEDSEQSRGMMQKKLVSKD
                     MTCSVVRNVIDLEGLRQRAQHPQAGAVIIFYGDVRNHSQQQEVSFLEYEAHENMALKQ
                     ISMVIDEARQKWVLHSVEVIHRLGKLAVKDCSIAIAVATSHRSDAYAASRYIIDTIKH
                     SVPIWKKEHFVDGVSAWSKGCEAYSVVEETAEPPPAVNNEVP"
     gene            complement(19245..20846)
                     /locus_tag="EYO68_03205"
     CDS             complement(19245..20846)
                     /locus_tag="EYO68_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HIC06844.1"
                     /translation="MRKKHKNILFITADQWRGDCLGCIGHPVVQTPNLDQLVRQGVLF
                     RKHFAQTVPCGPSRASLYTGLYAMNHRSVNNGTPLNNRFTNIAREVRKCGYDPTLFGY
                     TDTTADPRKLHQQDPLLSTYEGMIPGMSSGVTLTDNQLPWIADLITKGYDHSLNQYTV
                     FDPILNYPGAKGRGATFAPPVYPDKDSNVAFLTDELLKWLSVREDENWFAHLSYLSPH
                     PPWIAPEKYNNMYDPSEVPKPIRRESLEEEGRQHPLLKMLHELIPRSFFFNDKLIEPA
                     ALMSDLDVLQARATYYGLMSQVDHHLGRVFNYLKKSGKYESTLIIFTSDHGEHLGDHY
                     SFGKYGYFDQPYSIPLIIRNPEITKQTSDKSAVIGTQVELFTETIDVMPTILDWLETE
                     IPEECDGRSLLPFLQGNIPDNWRTEVHWEYDFRKIGSFQPIIEQQFGLSPDQCSLTVL
                     RDEHYKFVHMTALKPLLFDLQKDPEEFINRADDPGYQDIMLKYAQKMLSWQMLHRDRT
                     LVNMNMESGNLVNWKGPRILNKVGE"
     gene            complement(20875..21267)
                     /gene="gcvH"
                     /locus_tag="EYO68_03210"
     CDS             complement(20875..21267)
                     /gene="gcvH"
                     /locus_tag="EYO68_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIC06845.1"
                     /translation="MSEIPKDNLYTRDHEWIRIEGDKAEIGITDHAQKALGDIVFAEL
                     PEIEDEIDAGDEFGSVESVKAVSSLFMPVSGRIIAVNTELKDSPELINEECYDDGWVV
                     RIELSNPDDSAELLSPADYEEFLLDDEA"
     gene            21613..22992
                     /locus_tag="EYO68_03215"
     CDS             21613..22992
                     /locus_tag="EYO68_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIC06846.1"
                     /translation="MIRASKTRAALVQNVQKAVVHIKVEKIMRSPDGRALNNPRDLYN
                     DEFFRRFFPELQPPDNQQPKKREQPNREFRQKGMGSGSIIDAEGYILTNHHVVGEADE
                     ILVVLYNGDERKAKLVGTDPESDIAVVKIEGNGLPVLPMGDSDKILVGEDVIAVGNPF
                     GLIQTVTYGIVSAKGRSNVGINEYENFIQTDAAINPGNSGGPLVSLRGEIIGVNSAIF
                     SQSGGYQGIGFAVPINMARKIMRDLIEKGTVSRGWLGVGIQDVSHDLAKAFKLKSAKG
                     SLITGIMQDTPAQKAGMRKGDVVIRINDKLIQNSNHLRNEIANAGAFAEIEMELIRDG
                     KTVLLKLKLDERPKKIGRMKTSPEPTQTPEQLELLGMTVEELTEENAKQLGIEPGIGV
                     VISNVKSGSPAEKSGLLPGMIVQEAERQAVSNIDTFKEIINDIDQGKGILLLIRTSNG
                     SRYIFLQEN"
     gene            23232..23564
                     /locus_tag="EYO68_03220"
     CDS             23232..23564
                     /locus_tag="EYO68_03220"
                     /inference="COORDINATES: protein motif:HMM:PF04024.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIC06847.1"
                     /translation="MNLSFLLVGLSSLFFALWTAYGLYRRYKGTLPERPAQRKRWFGV
                     CADFSETVGIPVSIVRFFVLFYTPVGLGPVFYFIYYLVIRNKEPIPLKSPERNLQISK
                     IESHYFGS"
     gene            23571..24395
                     /locus_tag="EYO68_03225"
     CDS             23571..24395
                     /locus_tag="EYO68_03225"
                     /inference="COORDINATES: protein motif:HMM:PF02602.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIC06848.1"
                     /translation="MTSVSYQHSEKFPLAGLRFLVTRQDSTESSLSGMLESQGASVLT
                     AQMTQIIPTESWESFDETVQQISNIDWAVFTSRNGVTHCLSRLNDLEVSAQQLFSSIK
                     TACVGQATASVLTDNGITPELVPEHFQSEGLIDAFKQHDLFEKRCWLIQAESPRKILR
                     DSLQKMGAQIILTPVYRNVPVVGDYTFLLAELEQQKLDWILFASPSAVQNFHQILPSG
                     FWESLAAEPKIACLGKITAKAVRDFGWSVQAAPNIQDFEHLVQSLCEINSQISEKT"
     gene            24392..25600
                     /gene="serB"
                     /locus_tag="EYO68_03230"
     CDS             24392..25600
                     /gene="serB"
                     /locus_tag="EYO68_03230"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIC06849.1"
                     /translation="MSDSQNLLVTMQGPVSEVDVLEHFTVHLEHSAFSIRDIHYINSQ
                     SALSLLMRLELADSADIAVLRKELDIAAKKYNLEIQINPLNEAPAASPVYRYILTLLS
                     QQLRPELLTKLFRQLRERSLHVAGISPLEADDLHVFEIEIHAEKPIDRQQLMHGLLEL
                     KSEYQLDLALQADDLFRRNKRLIFLDADMTFIQCEMINDMSHLAGKEAEVAEITHRAM
                     DGKINFDDALRQRVALLKGVRLSDLERLILNIPYTPGVERLVYILKTLGYKIGIVSGG
                     FTRVIDHIKQRFDLDYGFANTLEVKNGELTGGILGDILDGHQKGLILREVALKENILP
                     EQVIAVGDGANDLEMLSSASLGIAFNAKSFLRERAAGSLSLPNLDALLYFIGISRNEV
                     NALFKRERVK"
     gene            25597..26925
                     /locus_tag="EYO68_03235"
     CDS             25597..26925
                     /locus_tag="EYO68_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06850.1"
                     /translation="MNENESKYYSIEEIRKFQERGVQVLDSSSVFISRDVEPENILPG
                     CIIHPCSRISGAKTQIHSSAHIGVSGPATIENSWIGENAIVGNLGPVTLKDTVVGPQT
                     ILGSGVAENAVFLGKETMINDFTTGFGFRVRKGSLYEEDSSSAQHTDTKMTVLFPWTT
                     LGSNINFCDALISGGTGPELGYFSEVGSGSIHFNFTIRGDKATASLFGDVCQGLFLDQ
                     DRLFIGGNNTLLGPIKADFGVMTAAGSRSNGTLSPGLNFGHSLPKGKIDYEPRIFSGA
                     VGIVTKQVDVLAELTALFHWYQQVRIGCISQTTEQKFVYESGLNIVELNYKERLFQLG
                     RYVEALEGSLSIFSGSNKMSKKETAEQRQLLEKWPKIQQQLATPKAFELLIPESLTNA
                     IARKLAEGKLDYTVIIKGMDIEGKQKGKDWLNTIANGVRNIINSEIAMDG"
     gene            complement(26966..27322)
                     /gene="rplT"
                     /locus_tag="EYO68_03240"
     CDS             complement(26966..27322)
                     /gene="rplT"
                     /locus_tag="EYO68_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIC06851.1"
                     /translation="MRVKRAVHARKRKKSLFKAAKGYRGGRSRLLRTVKDAVDRSRES
                     SYRDRKVKKRDFRRLWIVRINAAVRLHGMNYSQFIYGLKAADIEMDRRTLAYLAMEEP
                     TLFAQVAEEAKTKLAA"
     gene            complement(27339..27533)
                     /gene="rpmI"
                     /locus_tag="EYO68_03245"
     CDS             complement(27339..27533)
                     /gene="rpmI"
                     /locus_tag="EYO68_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIC06852.1"
                     /translation="MKMKTHRGAAKRFKKTGGGGYKRKRAFLRHGMRKRNQDTKRVLR
                     KKAMVSAADSNSVDMMLPNG"
     gene            complement(27541..28086)
                     /gene="infC"
                     /locus_tag="EYO68_03250"
     CDS             complement(27541..28086)
                     /gene="infC"
                     /locus_tag="EYO68_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIC06853.1"
                     /translation="MRRSRPPTRNEESTRINDQIRVNEIRLIDGDGEQVGIISTDDAL
                     EKARVANLDLVEVSPKAKPPVCRLMDYGKFKYQQSKRNHVARQNQKVVHLKEVKMKPK
                     IEEHDFQFKLRNALRFLEDGDKVKVGVRFMGRQIAHKDLGVELLEKFQAEIGDDGTVE
                     QPISSEGRSMHMILAPPKKRK"
     gene            complement(28121..28195)
                     /locus_tag="EYO68_03255"
     tRNA            complement(28121..28195)
                     /locus_tag="EYO68_03255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(28161..28163),aa:Val,seq:gac)
     gene            complement(28225..28340)
                     /gene="rrf"
                     /locus_tag="EYO68_03260"
     rRNA            complement(28225..28340)
                     /gene="rrf"
                     /locus_tag="EYO68_03260"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(28416..>29803)
                     /locus_tag="EYO68_03265"
     rRNA            complement(28416..>29803)
                     /locus_tag="EYO68_03265"
                     /product="23S ribosomal RNA"
BASE COUNT         8571 a         6152 c         6333 g         8747 t
ORIGIN      
        1 tctcactacc atcacatcat aaaccgacat attgctcaat ccaatccgtt gaattcattt
       61 tggcattact tattctaaag accttaatag acttaatgtg tttcgggaat aatcaggagc
      121 cctaaactgt ggcatacttt cacgggatca gttaaaatgc tgaattaaaa ctcatttacc
      181 aaggtgccaa gttgaaaaat aatatttatg ctaaatggat tttcggggca atttattcag
      241 attcatgttg gctggaacaa gccaaagaac agttggaaaa gcagaactgg aaaatccagc
      301 accaatctcc ggaattcact tttgaccaaa cggattatta tgcatcagaa atgggcacta
      361 ggttgaaacg ccgtttcctg agtgtcagtg gtatttacag catggaatca tctgctgatt
      421 ggaaattaag aatggttaaa atcgaaaatc ggttcagacg caacggcaag aggagaataa
      481 acttggatcc cggttttctt gatttacacc gtgttgtatt actttccgga aaagaaggac
      541 cgcaaaaaat ttatctgcgg gatggagtat gggcagatct tgttttgctg aaaaacaaag
      601 ggggatatcg agatttagca tggacatttc ctgatttgcg ggatggtcgt tacaatgatt
      661 tttttttgca ggttcgtgca gagtttaagg aagaaaaatc tctcagctca aatatttcat
      721 gaagtaagac catttttcag ggagggtccg agaaaattag ggtcttttga aaataaatta
      781 agtttggttc acggtaagaa ttatttacaa aatcatttag tagtttataa atattcaatg
      841 gaaatatttc caaaaattgt tgtggtagcc ggaccaacgg caagtggaaa aagtgatttg
      901 gcacttcgac tggctcagga atttgatgga gaattgattt gttctgattc aatgcaggtt
      961 taccggcaaa tggatatcgg aactgctaaa ccaactccgg atgttcaaaa aatagttccg
     1021 catcatcaac tggatctcat tgatccagat gaatcatatt ctgccggtaa atatgcaagg
     1081 gatgcttcca gtattattga gaaggttgcg gcacgcaaac gtctgccgat attggtagga
     1141 ggtacaggtt tgtattatcg tgcactgatg tacgggatca gtaaaattcc atcaattcca
     1201 gaaaaacata gaaaaaaagt gacaggctgg cattctgaac atggaacata ctattgttgg
     1261 aaagaactgc aaaaacttga tccggaaggt gctgtgcgtc tgcatccaaa tgacactgca
     1321 agaattttgc gcagtctgga agtagtgctt tcaacaggaa caactctagc agaatttcaa
     1381 caggataaac cgtttgcgga agctaaatat tcttttcatg cagttgcgct tgaatgggaa
     1441 cgtgatgttc tctatgaacg tataaatcag cgcacacaca tcatgctgga gtcaggatgg
     1501 attacagaag tggagaaact gttgacccgt tattctcctg aactaaaacc tcttcaggca
     1561 attggttatc gggaggttat tcagcatctt caaaacaagt tggaatgggg cgcgatggtc
     1621 gaagaaatac aaaaaagaac tcggcaatat gcgaaacgac aaatgacctg gttccgtaaa
     1681 gaaaataata tcggatggca taaacctgat gacattgacg aaatcttgga taaaatcaaa
     1741 gtttaccttg aaaaataggt gtttttagag gcagaataag aggattgatt tcaacttaaa
     1801 tttataacaa tcattaaaaa aactaaagga gcctttgtga aaacctatac cgttgatcat
     1861 caaaactacc atatattcaa aactggaatt ggtgctaaaa aacaatttgt tcatttccag
     1921 tggggaaagt tcgatttcag aatgagcttt atcatcttaa caaatataaa acaggacaat
     1981 cctgaaaaga caatttctgc aaaaaacggc agtaaatatt taatagagaa atttgaagtt
     2041 ctctaccaga acgaatggtt tgagttcata aaaccaactg cccatggcat gcagttagaa
     2101 gaaactcttt ggcacagaaa cggacaagat tattacgtgg aatttcctaa agatctcagt
     2161 agtgtggcgt tggagatttg tgcagaagag ctagagctga aagtcctcca ggacgtggca
     2221 gcttaaaaga gtaagtgatt tataaaaacc agaaatattt attgtaacgc cctgtccatg
     2281 agttgaacaa taattaacca attattacaa atacagtaat ggaagaagct tcccaaaaca
     2341 cggaaactgt gaagaaagtt tttattcaaa cttacggttg tcaaatgaac gaatatgaca
     2401 ccgaaaaaat gtttgaagtt ctcaagcatc aaaattactt tcaaacagac tcacgggaag
     2461 atgcagattt aattattctc aatacctgct ctatccgtga aaaagctgag aataaggttt
     2521 acagtgaatt gggccgctta aggaaactta aatccgttaa accggagctg aaaattggtg
     2581 tcggtggatg tgtggctcaa caggaaggta aggcaatttt acgtagggaa gctagcgttg
     2641 attttgtttt tggaacggat aatttatttg aacttcctga aatgctcaaa aaagtcagcc
     2701 gaggtcaaga aataattcaa acaaaacgac atgctcggca gaaagtgcgt aacttcattc
     2761 cggaatatgc atttcaacaa actcaacatc acggaatcaa agcacatttg gctatcacaa
     2821 agggctgcaa caaccattgt tctttttgtg ttgtaccatt tacacgcgga atagaagtca
     2881 gccgtgaacc agaaaatatt ttagcagagg cacagcgtct cacttctgct ggaacaaggg
     2941 aaatttgttt gctgggtcaa aatgtaaatt cctacaaggc taacggagtg gattttgtag
     3001 agcttttaca ccgcttggat gagcttgaaa atttgcaacg tctgcgtttc atttctccac
     3061 atccaaaaga ttttcatgaa gaattagctg atgcatttgt aactttaccc tctctctgcg
     3121 aacaaatgca tctgcctctg caatccggat ctgatcggat actccgtaga atgagacgct
     3181 ggtacacgat ggaaacgttt tatgaaaaag tggctatgtt gcgtgatcga cttcctgagg
     3241 ctacgctttc tacagatttg attgtaggtt ttcccggaga aacagaggaa gagtttgaaa
     3301 tgacaatgga ggcagtgcgt accatacgtt ttgatctaat ttattcgttt aaatattcaa
     3361 cgcgtccagg aacccgtgct gcagaatatc ctgaacatct tgcagaagat gtgaaatcaa
     3421 agcggcttca aattttactt gaaactcaag gaaaaatcgt acaggaaaaa aatgaagccc
     3481 tggttggctc cattcaagag gtcctggttc aagggaaaca ttaccgcaaa aattctatca
     3541 ccggacgtac acgaggtaac cactcagttc acataaacga ctcttgtgct atagcaggag
     3601 accttttgtc tgttcgtatc acgggtgcac ttcacaattc cttagaagct gaaattattt
     3661 ccaagagtac tgttttaagt taattttatt aataaccaga ttggtctcca ggttattttc
     3721 aagatttgct gggagtttgt gacctttatc ttatttggtt tgaggacgtg tcagtggatt
     3781 cggcgcggtg cgaggtgata atttgcgtcc gcataattca tgatcactgt aatttattac
     3841 acggcgcaaa ggtggttttt cagaaatgtg aatacagctg tctccatgat ttttccagac
     3901 tgctgcgctg cagcccaaca cagttgttgt acttgccacg aaaataattt taataaaata
     3961 tctgcgaatt gaatatttta taaataaaaa taatgacttt gttggaaata acatggaaaa
     4021 aattcgcagt ttgaggaatc ttaggattat tattctataa gtcaaacgtt tgtccgagct
     4081 aaaaatccag ctattgtttt aactcactat aaaaaattct ggtgtttatc tggaagagca
     4141 atctggatgc cacgtttttt taatctgata tttacgagtg attgtgaacc gaattggtgt
     4201 aggtgtttca aaagagactg atccggatgt tgcaattgca gaagtattgc actcagcagt
     4261 caaacaggca aaacttccaa aagtgaattg ggtgctgact ttttttactc ctgcacattt
     4321 tatccatgca ggacgtctgc atgaattgat tcgggagcaa acaaattgtg attgtatcac
     4381 cggttgcagc ggaatgggag tgctttcagt actaggagaa atctcaagtg gtccaggatt
     4441 ggttttaatg gcgggctata ccccggagct acgtccgctc gcgatagcga aataccagga
     4501 acttgaacac agtgctgggg ttactcaaca attccgtgaa acacttgaga actttggtgg
     4561 tgagaaaccg ctatttttct tctttccgga tgtttatcag catcagccgc acaattttat
     4621 caatatgttt aattttctca aaaaccatcc gtctgtattt ggggcaggat cttgtaatga
     4681 cggcagccag gaaacttcca ttcaatttgg tccagatatt gttactctca atggtgcagg
     4741 tggattagcc ttagacggtg tgcctgagtt tagtgcggga gtaacacagt cctgtgcgac
     4801 attcgaagag ccaatgttta tcaccgaaac aaaagacgat ctaatccttt cactcgatgg
     4861 gaaacccgct ttggaagtgt ttaacgaagt agctaatgaa ttgggatttt caaatttgga
     4921 gttggcagca cagcaactgt tgctaagttt cccacttgat cctgagcagc ctgttttcac
     4981 cggagaaggc tctatggccc gacatgttac caggattgac gctgcatccc aaggcatcag
     5041 cacatcacaa attgttcacg agggcggagt tgtttctttt gcctatcgca acaatgcaac
     5101 tgctgaacaa gatttgcagg caatgctttc ccgtttgaaa aacaaaaata cgaatacacc
     5161 aacttttgga atttatttta attgcgcatc tcgtggcgaa gcgctttatg gacgacaaaa
     5221 tgtggatacg caattaatta gggaaacgct gggagagttt cctctgatag gtttttttgg
     5281 aggttatgag ttggcgcaaa tgccgcaagg agtacagtta tacacttata ctggcgttct
     5341 tgttttggtt tatctagaaa ataaataaca aaaccctgca agaaccgctt aaattacgtg
     5401 aatatttgta catacaaaac caaatagaaa aataccaaaa aagaactagg tattaccgat
     5461 gtttgtaagt gttccataag aaaaagttta ctatagtcaa agggttatct tgaaaaactt
     5521 actgtgtcgt tctgccaaat ctgcggctaa aaagagagtt ctattttcga attatttttt
     5581 tgggattgca attgcactgt tgctgtccgg atgcggttat gtgattgaag gaagtaatcc
     5641 ggtactgcct aacgaagcac aaactcttgc acttcttccc atccaaaatc agacgtttaa
     5701 agcaggtttg gaaacagatt tggttgagca actgagccga ttgttgcgct caaacagttc
     5761 agtaaaaatg ttgacttcag gacaggcgga tctcagactg aaaattactt tgctaaaatt
     5821 gaaaacgagt tcctcaggat tgagcaaaga ccagatttca acaggtgtga aagcgatatt
     5881 acaaggtgag gtaattttgg aagacatgcg tactggaagt aaggtgtggg aggaaaaaat
     5941 gctccaagtt aaactttctg aatccggagg caatgactca gacactgttt caagtgtttc
     6001 actttctgga agcatacgtg aattaatcaa acaatttgca aaaaaactgt ataaccgcct
     6061 ctttaccact ttctaattta tgctataagt atgaaacccg catatactaa acgcattcat
     6121 agaaatgaca gcatatttca aaaagatcat ttttgacaat cgcatccatt tttttattag
     6181 tgaaagcttc tgtttagtat aaaaacagtc aatccacatg ttgtaaaaaa acaagcatgc
     6241 atgactcttt tgaaaccggt cttccgcaaa attctgcaaa ttacactccg ctttcaccga
     6301 ttacattcct aaaacgtact gccttcgtcc atccacatcg gacttctgta attcatggga
     6361 agcatcgttg gacctgggcg gaaacataca tccgatgttg cagacttgct tcagccttga
     6421 gcaaacgtgg tataggcaag ggtgatacag tgtctgtaat ggctccaaat attccggcta
     6481 tttttgaagc acattttggg gtgttgatga caggtgctgt tttaaacact ctaaatatac
     6541 ggctggaagc agaaacgctg gctaatattt ttgagcatgc ggaaacaaaa gttttactga
     6601 ccgaccggga attttctcca cagatcaaag tggctttatc caaagtgaag cgggacatcc
     6661 tggtgattga cattgatgat ccggaaacgg aatccggaga atatttgggt atgcttgaat
     6721 atgaagcttt tttggcagag ggtgatccgg aatttgaagc agtgctgccg gaagacgatt
     6781 ggcaggcggt ttcactgaat tacacatccg gaacaactgg aattccgaaa ggtgtagttt
     6841 accacacacg cggagcttat ctgctggcaa ccgggaatgt gttggcctgg gaaatgccac
     6901 accgccctgt atatctctgg actctgccga tgtttcattg caacggctgg tgtttcccgt
     6961 ggacaatcac aatgctgggc gggacacatg tctgtttgag aaaagtaact gcaaaaaaca
     7021 tttataactc tgttgctgaa caccatgtta ctcatctttg cggtgcaccg attgtgatga
     7081 acatgatcag caatgcgcct aaagaagagc agcgggaatt gccgcacagg gtagaattta
     7141 tgacggctgc ctcaccgccg ccgccgactg ttattgccca aatggaagaa gccggattta
     7201 atgttacaca tgtttatggc ctgaccgaag tttatggtcc ggctgttgta tgtgaatggc
     7261 aggaggcttg gacggaaaaa gacaaaacta ctcaagcaca actcaaaggc aggcagggtg
     7321 tgcgttatca tgtgctggaa ggattgacag ttgctaatcc tgaaacactg gaacctgttc
     7381 cggctgacgg tgaaacaatg ggagaagtac tgatgcaggg aaatatagtc atgaagggct
     7441 atttcaaaaa tcctgaagcg acagcacaag cttttgcagg cggctggttc cattccggag
     7501 acatcggagt actgcatccg gatggttaca ttgagttgaa agaccgcagc aaagacatca
     7561 ttatttctgg tggagaaaat atcagcagtg ttgaaattga aaacgttctc taccagcatg
     7621 aggacattct cgaagctgcc gtggtggctc gtccggataa aaaatggggt gaagttccct
     7681 gcgcttttgt ttcattgaaa accggttgtt cgctaaatga acaggaggtg agaaaatttt
     7741 gcaggacaaa actgtcttta tttaaaattc ccaaatacgt tgttttttgt gaacttccta
     7801 aaacttccac cgggaaaatt cgcaaatctg tcttgcgaga gcaggcaaaa aagtcatagt
     7861 cttgtgaagg taactttagg tctgtccttt tatgaatcaa aaaattcaac tggttttgcc
     7921 ggattaaata atttttcttt caaaatagaa aatggataaa atgggcatga ccaaaaaaaa
     7981 atttattgaa gatttaaatc ttaatggatt gcgtgtcttg acacgtgttg attttaatgt
     8041 acccctcaac aaagatttgc aaattaccga caatgggaga attgaggccg ctttgccgac
     8101 cattcggtac atcgttgagc aaggaggaaa agcgatcttg atttcgcact tgggtcgtcc
     8161 gggtggagag cgggtcgaga gcctgagcct aaaaccggcc gcggaagagt tgtcactgtt
     8221 gctcgggcag cctgtaacct ttgctaaaga ttgtattggt gaagaggttg aggctctgat
     8281 tgaagggatg caaaacggag aagtgctttt gctggaaaat ttgcgttttc ataaagcaga
     8341 aacgaaaaat gagcctgagt tttgcggagc attgggcgaa ctgggtgatg tttatgttaa
     8401 tgatgctttt ggaactgcac atcgggctca tgcttctact gccggagtta caggatttat
     8461 tccggaatcc gccgtcggat atttgattcg taaggaactg cagttccttg gtggagcggt
     8521 tatgaatcct gcaagacctt ttactgcaat tttaggaggc gctaaagtat ccgataaaat
     8581 taaagttatc gacaaattgt taaacaaagt tcaaacttta ataattggag gaggaatgtc
     8641 atgcactttt ttgaaagctc aaggttacaa aattggcgct tccctgttgg aagaagactt
     8701 attggatttc gcaaaagaac tgatacagaa ggcagaggaa aaaggtgtta aactcctgtt
     8761 gcctgtggat gcggtaattg ctgacagttt tgacaaaagt gcttcaactt gcagtgtttc
     8821 tgtagaagag ggtgctccgg atggttggct tattttggac attggtccaa aaagtgctga
     8881 aacattttgc gactccgtac gtaaatcagg aactgtcctt tggaatggcc ctatgggggt
     8941 ctttgagatg gaagcctttg ctcagggaac attcgcaatc gcacaatgtc ttgcggaagt
     9001 gactgacaaa ggtgttgtga caattattgg aggcggagat tccgcagccg cgatacaaca
     9061 atttggactc agtgagcgga tcactcatgt ctccaccgga ggtggtgcgt cacttgaatt
     9121 tttagaaggt aaagaacttc cgggagtgag tgcaattcag caatcaaaat aatacttcat
     9181 gctgtactca ctcagcagca cattcaacag taatttatgg acaaggacac ggcacttcag
     9241 gaagacaacg attcgacaga aaatcaggag gaaaattccg aagtaactac agaggagaaa
     9301 agtacgaatg tttctttcag ttttttcctt tatcgtctga tagcttatgc tgatgcccgc
     9361 cgctcagttg cttcttttgt aatcattctc tttcttgtta cattcagtga catacttttt
     9421 gagaacttcc tttttgtgcc gtatgtagaa ctggtagagt cactttcagg tgtgcatcca
     9481 ggaggctttg ctgagttgga cgtaggattt gcgcctgagg tttggcaagc tctgctggga
     9541 atggttctgg gaacacttat tttggtgatt tcgattgcgt ctcaaagcat accgaagcta
     9601 atcgatttct acatgagaga tatcccgagt ttgctttaca tctggtttct aattatttcc
     9661 ggcgtgcatg cattaatcat taaaatatat ggcgaaatcg gtctggttcg tgaaccaagc
     9721 cggattttca acacccattt cctgttgaca atttgtacaa tcatcgcctt cccctatgtt
     9781 ttctatatcc tgcgctacac caaacccacc aacatcatta accgtattta taacaacaat
     9841 atggaccaga tcagggcact cacttcatcg agaaaccgtg cgttggccca tattccaaag
     9901 gtcgttgaat atcagcaata cacaattttt gaggcactta atcaactgga cgatattctt
     9961 gaattctctt ctttcaagga gctgaaggca gatattgttc acgatatgag tgtgaccctt
    10021 caaaattaca tacggctcaa acgtgacatt gctcccggtt tcttcaaagt ttctccaaaa
    10081 gttcgcaccg atatttcttt caaaactatg gtggggcaat ttggagagat ggaacgtaac
    10141 caaagttttt atgaacagaa atgcttccgt ctgctgggca atgtttatat tcgcttactt
    10201 gaacatggtg aatttgattt atcttcgatg gttgcaggtg aaatggctaa tttaggttta
    10261 actgcaattg aagaggataa tactgaattg attgacatta tcatcattcg tttcaacaca
    10321 ttactgcgtt ttgcaataaa gcatggtgtc cggaacaatg aaccgcggaa cctgtataac
    10381 cttggatttt attatggaaa ttttatcaga tatttggtgg agcataaaaa gacagatcac
    10441 gtaaagcgtt gtttcatgta tttgcggatt tacggagtag aaatttttaa gcatggaagc
    10501 aacagtcctg caatgtattt catagtggat gtgattgcca cagaaatgaa aaaagtactg
    10561 gagcagattt atcatgatga ctgggacaaa gaaatacaga atgggatgct cagtgaaata
    10621 cttcaggtgg atagtcctcc agattttaat aaagaagatc ttgcccgcgg agtacttatc
    10681 aacaatggtg tgcgtgttct tcaatttgga ctggccttat tctatcagcg ggagggaatg
    10741 actgattttg tagaacgtat tgccaaagac gtactggatg atcttcaggc tttaggagaa
    10801 gcaacttttt ctcaagtcat agaaatgact tcaaaccgcc tacttttttc cggaccaact
    10861 ttttgggagg atacagatag aggtaatcta aacatctatt acacttcaga tcaggatcaa
    10921 attgatggat ttaagaaacg actttatgat ttagctgaaa ctcagttgaa aaccgagatg
    10981 acgcagaaat atcagttgac tgaagtagaa ctgaatttac tctgggaaat gtcgcggatg
    11041 acgaaagaaa aggaagtatt tcaaattagc actaatgtta aaacttttga gttaattcta
    11101 aatgatttag agaaaattga tgaggaacgt ttggatgcat tggtcacttt gcgggaaaaa
    11161 ctgaatttca actctgataa cccaaaactc attgtcacaa ccagtcgtca gcttgcagtt
    11221 ggtacagaac tgaaaatttc tggaaaaatt tcagggcaaa caaagcagct tgagttccag
    11281 gcagttgctc agttgaatac tccaaattac ttatttgtta aagtgatgga tgagggagag
    11341 gtggttaaaa ttgaaaaatt atcatcacta accgttaagt tcaggccatt gcgtcaaaaa
    11401 atggtttatc aatttcatgc aactcttcaa aacactggag caaattctct gctccgtatt
    11461 gagcattcca acaaagtcaa aattgtagaa gagctttaat caaaaagtga agcttgtgct
    11521 tcaggaggtt caggattttt ttcgtcagtt actgaatcat caagcttctt caagcagtcc
    11581 ggattatcaa atgtggggac actgactttt ctggaaactt tccacgcctc cattttttca
    11641 gcaggaagag gaataaggag tttttctggt gcggtttctg ttccggaaag atcgagccag
    11701 ctcaatccgt tattcggagt caatataact ggcattctgt gatgaatttt ctgcattaat
    11761 gaatttgctg caactgtcaa aatggtacat gtacgcctga gttctccttc aggtgatgtc
    11821 caggaatccc ataatccagc aaatgccatc atactatttc ccttatcaga aatttccgat
    11881 ttcaagctga taaaataggg ttgtttttgt ccttcctctt tagcccattc ataaaaaccg
    11941 gtggctggaa tcaggcatct ttgtttccgg aatgcttgcc gaaagaaagg tttttctgaa
    12001 accgtctccg cacgtgcgtt gatcatgggg gttggttctt tggcccaaaa aggaataaaa
    12061 ccccattgac gcatttccaa actgcgtaaa ccgtcctgct ggacaatcac aggaatatct
    12121 tgagaaggac aaatgttgta gcggggttcc agcatcattt cagattgttg gatttcgaaa
    12181 gtctgcatca ctttttcctc gctctctatc ataacaaatc tgccgcacat ttctaaactc
    12241 cttcaattcc gctccggtat ttctgcagga aggttttttt gaaatttgga aagcgcttct
    12301 ccttgattgc attacgtgcc tgcttaatga gggagatata aaaaaacaga ttgtggtagg
    12361 tgttgagtct catacccaat atttcattgg ttttgaacag gtgacgtaaa taagcacgtg
    12421 aatagttctt acaagtagaa caagcacaat ccgggtccag tggagctgga tcttccttgt
    12481 attgattttg cttaatactg atttttccgt gtgaagtaaa aagcgagccg ttacgggcgt
    12541 tgcgggttgg catcacacag tcaaacatat caattcctgc ttcaattcct tccagcaaat
    12601 cctccggatc tccgacaccc atcaggtatc ttggtttttg ggcaggcatt tttaccgcaa
    12661 tatgatggac cacgctatac atttcctctt ttgtttctcc tacactcaaa ccgccaattg
    12721 catagccgtc aaagccaata ttcaccattt gctccaaact ttgctcacgc aaattcaact
    12781 cacctccacc ttgaacaatt ccgaacaacg cttgttctcc ggaaactcct gaaccttgac
    12841 tgctggcatc tttgctacgt tgtgcccatt tggtagtcag ggcaatcgag cgttctattt
    12901 cttgacgggt tgcaggcagt tcaaggcact gatcgagaca catcatgatt gtcgacccca
    12961 ggttttgctg aatagcaata gaaatttcag gtgtgagagt tatttttgag ccgtcgatat
    13021 gactacgtat cactacacct gactgatcaa gcttaagcag tttagccaat gaaaatatct
    13081 gaaatccgcc gctatcagtc aaaataggtc catcccagtt cataaaatga tgcaaacctc
    13141 ccaacttgcg taccagttca tcaccgggac gaagatgcaa atggtaggtg tttcccaaaa
    13201 tgatttcggc acccaactca ctaacctctt ccggggtcaa ggccttgacg gttccaagtg
    13261 ttccaaccgg cataaaaacc ggtgtatgga ttgtaccatg tagagttttc aaaattcctg
    13321 cacgggaatt atggtctttt gcctcaagtt caaactgaat catacaggac ttcagttggc
    13381 gggaataatg ctctgcatta aggaaattgt ctgttcttgc tgccttttga aaagttcttc
    13441 ttcagtttca cttacaggtg gaatgtcagg atctatttga aaaagtactt gactccgggc
    13501 aactattttt cctgtaccgg catcaatgca aattttccga attgttccag ggaactccgc
    13561 acgaatttga ttgaacattt tcatcacttc cagcaaccct aagacgtctc cttcttcaac
    13621 atgctgacct tccctgacat attcgcctgc ttccggagtt tcgcgcgaat aaaatgtccc
    13681 acctgtccat gctaaaattt cattgctctt tgcagacggc gggggtgcaa gcttcgcaat
    13741 caattctgca ttagactcag gatcctgaaa ttctttagga atttttactt ccagagtgtt
    13801 gttacctcga aaatcataat aacctgcttt ttttgcaaga attgacggaa gctgcaataa
    13861 ttccatactc atttgatgtc ctttgtgggc tgcacttata ccctcccaca atttcaggtt
    13921 tttacctaat ttaaattttg caggaggttt ttctgcactc agtatttcca tcaactcttg
    13981 ccatgataaa ttggtctttc caaatctggt ttttaattca ctgtaaaaag cagaggcagt
    14041 ttccagcaat ttttgatcgt tgctccagat ctgctcactt ggaggaactc cagaatgttg
    14101 ctcaagacgt aagaaatgat aaaggtcggc taaagtttgt acaggattct ggaaagttga
    14161 aagtttgctg tgaaggtttt ttgatttctg aaaagacagc cagcccatca ataagtgagt
    14221 cttgttcaac aactttttga gaggccgcaa gataagagtt attttttgat cataaatttg
    14281 gagcccatca ctgccgtagt ttttttggac ctgatcacgt tgaatcttcc aggccagatc
    14341 cagatttatt tgatcacaaa agtttttcaa ttttccagca agtgccagat acgaagatac
    14401 aaaacgtgtg tttggtttaa gcatcggatc gttgcccagc agccagtgta ataagccata
    14461 gtggaaatcg acattcaaat gcaaatcatg tcctcggact tccatacaac gtaaaatttc
    14521 agtaagtttc tcaaaacttt cccgacgtga aattccatgt gtaatggaaa gagcaacgtt
    14581 tgaatcataa gcacctgcca agttgtaggg ttgcaataga cctgtatcag gattgcttat
    14641 cccaatcccc tggtcatcac gtatttcatt ttcagttgca gcagaccaag ctcttaagac
    14701 tcctcccgca tgtggtttta gtgcgggatt tgtcgcattc agacgggctt caacacctga
    14761 aacaaaacgt ggcaaacgtt taggacgata aagtttttct ccgtaacaat ttattaacaa
    14821 cattgcagca accagggaat taacaacaaa agtatcttca ggattgtcag gatttgaaaa
    14881 ttggagctga taagccattt ctgtgactcg atgttcaacc tgtatccgtg tgtttacttc
    14941 cataaaaaaa tgtttttctc cttcgacaat acactcgaaa gttgaaaccg aatcgagatt
    15001 cagtgctttg ccgaattcct ctgcctcttt gcacattgaa cttaacattt ttgcatcaga
    15061 acgtaacact ttggcttgtg gggttttccc tgctgattca tattcacgtg cggctgcttc
    15121 cagcatttct tctgtcaaag aaacttcgag taatttttgc tcgtgcattt gcagagaaca
    15181 atcacgtccg ccaagttcga tacaccactt tccattcccc agcaattgaa tttcattatg
    15241 gcgggtattc tcaatgttca attcaatgag aaaattcttg ttgtcaccct ctccggtagc
    15301 ttttgattca atcaaaactg acatcacagc ttcagctatt tcttctacag aagttacgac
    15361 tctctggccc ctaccccctc cgccgcccac atgtttgagg cgtatgcgct taccaggatt
    15421 ttctagccaa attgactcaa cttgccgaac agtttcttgc tgtaattctt caatcgtaaa
    15481 caggtcgatc tgcttcgcat atgaagcctg caaaatggtt actgcctgat caataagttc
    15541 ttcggattgc cagttttcag gaagactcag tttatgattt ttgataattt tcgggaaata
    15601 tgtatccggc gcttttcctg ccttacgcaa taaagtcagc gcgtctattc tgtcttctcc
    15661 aggtgtaacg gaaacattta gcgaacgcgc aagctgcttg gcttcatcct ttgatccggc
    15721 ctgttgaatt actttggagt tcggtccaat gaaactaatt tctgcattct caattcgttc
    15781 tacaaattct gcatcttctg ccataaagcc atagcctgca aataaatgcg tgtaaccatt
    15841 ctcatgacaa atatcaatta cctgttctat ccgctcaact ctttcctcct ttgtggcacc
    15901 catgtaatct gggacatgat gtacctgttt acggcggttt ctaattgcac gtaattctgg
    15961 agcaagggtt tgcggaaaaa tgatgctgtc tttttcagaa agcagcatgc cataggttgc
    16021 ccctagttct ttgaatatat tcattgtttc taaacgaatc ggccccctgc agacaattag
    16081 gcacttgaca tgattaaatg tgaaagacga ttgccaggaa ctttcaggat caaaagtgat
    16141 tgcgctcatg aaatgtttaa attatgaaaa ttatgtcttt gtttaaaatt attcgaaaag
    16201 ttttactaaa ataacggatc aaaagagatc tgagaatcac gctatttcta ctaaagttaa
    16261 taatcgcaaa ccggaatatg tttgcctgaa atttattaat tttgtttagt tcaggtactt
    16321 tctgaagctg gtctgggaat aatcaggcaa aaaacttttc ccacaattta tacattctaa
    16381 aacgccccaa ttgccctttc agagcttgaa tttcattaaa ttctttaatc gagtcagcac
    16441 gaataccgcg cccatgcagc aaaactggta ctggatcatc cgtgtgaact ccttctttgc
    16501 tccaagttgt atgatcggca gtgatgcata aacgcagttt accgggccac tttcctaaaa
    16561 accgtccaag ttctgtatca attctttcca gaaaatcttt ctttttttct gcatctctgt
    16621 tgtgtgcagc aatgtcacaa ccttttatat gcaaaacaac aacgccatat tgcttcaaca
    16681 attttgcagt gatttcgaat tttttattta aatcagtatc caggtttgca gtcatttccg
    16741 gagttttaca gacatccata cctgtgactt tggcaattcc gagaatagtg tcatctcctg
    16801 ttacacaaat accggacaaa cctaaaccgg aggggcgttt caaaattggg aaagcatgaa
    16861 cttgtcctgg ttctcgggtc aaaatcgaat ttgcaggtaa aagtcttttt gattttcgtt
    16921 caagatttac tggatgggtc gctaaaattt ttcttgccgc aagttcaaac aggtgcaaat
    16981 attttgcagt tcgcaaactt tttttgtcat ttttttttag tacttccgga tgtctaggtt
    17041 ttttaccaga gaggaagtga tctcccggat cacttccgga aaggcaatct ccaagcccag
    17101 atccccgtat cactaacgct acacgatgtt cagttccgga acccacatat aaacttacat
    17161 catcaatttt caatccacat aggctggagg acaattcttt tgtgccttca cgaatacgtc
    17221 ctgcacgacg gtcaacaatc attccttcgt catccagtgt agcccagttc ccacgaaaag
    17281 ctacgtcacc gggcatgatt ttcatgccac agccaattgc ctcaatcaat ccccttgcaa
    17341 ttgaaaatct atgtggatca tatcccagga ttgccaaagt tcccataact gttgttgcaa
    17401 cagttccaga cctgactgga tctgcaaccc cgcattgccc ttccaatgcc agctggtcca
    17461 ttactggaag atttgctgcc tgcagaggtg ttttgtcgcc cagttcaaca attggacgat
    17521 cccccatgcc gtcaaatatc aatagcagtc gaggaattct ttgcacaaag aaaggtcagg
    17581 atttggatgt aagagtagat ttcacctgct cctgcagggt atttatgatt aattgcatca
    17641 gcgtgtttgt tgtttcgtca aaatcttcat tgtcgacaac ttcaatctcg ttgttttcac
    17701 tttctgaaat acaatattca tgaatagatc ggatattttc aaaactacgc aaatagggtt
    17761 ctttttttct caactcatta ccttttccac gttcgaaaaa tcgatctttg tggtgtttgt
    17821 cattcatcaa agaaagcgtg atgggaatgt gataggctct cttggttcct tcctgcaaag
    17881 tttgctgcat atatggcaac aaatggacac cttcgataat catacttgta cgctctttta
    17941 cagcacgatc aactacagct ttcactccca cactaacctg ctgggcctgc aaacaaaatc
    18001 cgctaattaa tctggcattt ttgtcaagcg tctttggcat tccaactccg gctttgcttg
    18061 aagaattgtg gagagaagga agtaaatccc ttttaaaaac gagccgcatt atttcacgaa
    18121 tagtatccgt acctgtaatt ttgttgatac ccaatcttcc tgctaagaaa gtggacatga
    18181 ctgatttccc tgtaccggaa gcaccagcga gatatattat cactggcctg tcaagttcat
    18241 ctatccgact cgctaattta tataaaccag caagatgagg actatatttt ttctttatag
    18301 tgctgaaaac ctcctcaaat aatttatttt tgctgattcg caggatgtct ttttttatca
    18361 aatcttcttc cagatcaaaa gcaattttgt atgcttcttc cggtttaatt cccgctgctg
    18421 tgatcgactg ggttaataat cctttagaaa atggtgaggt tgcgtttcta cgaaaaacac
    18481 agatttgttt tcctgaagga aacagttccg aggtaagcgt cctgaggagt tctttaccgt
    18541 agcgtgacac tatcaactca tccactgact gcatcaatga ttcggaagta acttcagttt
    18601 gctcctggaa ataagatttt gcatctttgg aaatttgata tgcctgatca aaggataatc
    18661 cacgttgcat cagtttgtgg gccagcattc ccctcatgaa ggggtgcttt gatttgtctt
    18721 ggcggataac ttgaagttgc atcatggaac ctcgttattc acagcaggcg gaggttcagc
    18781 agtttcttca acaacgctgt atgcttcgca gcctttgctc catgcggaaa caccgtcaac
    18841 aaaatgttct tttttccata ttgggacaga atgtttaata gtatcaataa tatatcgact
    18901 tgctgcataa gcatcacttc ggtgagaagt tgctacggca atggcgattg aacagtcttt
    18961 cacagcaagt ttaccgagac ggtgaataac ttctactgaa tgaagtaccc acttttgccg
    19021 ggcttcatca attaccattg atatttgttt cagggccatg ttctcatgtg cttcatattc
    19081 taggaacgag acttcctgct gctgactgtg gttacgcaca tctccgtaaa aaattatgac
    19141 tgctccagct tgaggatgtt gtgcacgttg gcgcaaacct tcaagatcaa ttacattacg
    19201 aactactgaa caggtcatat cttttgatac taattttttc tgcatcattc ccctactttg
    19261 ttcagaatcc tcggaccttt ccaattcaca aggttgccgg actccatgtt catatttacc
    19321 aaagtccggt ctcgatggag catctgccac gagagcattt tctgagcata tttcaacatt
    19381 atatcctggt atcctggatc atcggcgcgg tttataaact cttccggatc cttctgcaaa
    19441 tcaaacaaaa ggggcttcaa ggcggtcata tggacgaatt tgtaatgttc atcacgcaaa
    19501 acggtaagtg aacactgatc cggagacaat ccgaattgct gttctataat cggttgaaaa
    19561 gaaccgattt tccgaaagtc atattcccag tgaacttctg tgcgccaatt atccggaata
    19621 ttgccctgca aaaaaggtag caaggatctt ccatcgcatt cttcaggaat ttctgtttcc
    19681 agccaatcga ggatcgttgg catcacatca atggtttctg tgaataattc aacctgtgtt
    19741 cctatcaccg cagatttgtc ggaagtttgt tttgtaattt ccgggttccg tatgatcaga
    19801 ggaatagaat aaggctgatc gaaatatccg tatttaccaa atgaatagtg atctcctaga
    19861 tgctctccgt ggtcgctagt gaaaataatg agggtagatt catatttacc tgactttttt
    19921 agataattaa aaactcttcc caaatgatga tcaacttggg acatcaatcc ataataggtc
    19981 gcacgtgcct gcaaaacatc tagatcactc atcaatgcag caggctcgat aagcttgtcg
    20041 ttaaaaaaaa agctgcgtgg aattagttca tgaagcattt tgagaagagg atgctgccgg
    20101 ccctcctcct ccagcgattc cctgcggatt ggttttggca cctccgaggg gtcatacatg
    20161 ttgttatact tttccggtgc aatccaggga ggatgcggac tcagatagga aaggtgcgca
    20221 aaccagtttt cgtcctcacg aacacttaac cacttgagca actcatctgt caaaaaagct
    20281 acattactgt ctttatccgg gtacactgga ggcgcaaatg tcgctccacg tccctttgct
    20341 ccgggatagt ttagaatagg atcaaaaaca gtgtactgat tcaatgagtg atcataaccc
    20401 ttggttatca aatcagcaat ccacggaagc tggttgtccg tcaaagtcac tccgctgctc
    20461 attccgggaa tcatcccttc gtatgtgctg agcaaggggt cctgctgatg taattttcga
    20521 gggtctgctg tagtgtcagt gtagccgaac aatgttggat cataaccaca tttcctcact
    20581 tctcgggcaa tgttcgtaaa ccgattgttc agaggagtac cattatttac tgaacggtga
    20641 ttcattgcgt acagtccggt gtataaggaa gccctggatg gcccgcaagg tactgtttga
    20701 gcaaagtgct tacgaaatag cactccttgt ctgaccagtt gatccaaatt tggtgtttgc
    20761 actaccggat gtccaataca tcccagacag tcaccgcgcc actgatctgc agtgatgaat
    20821 aagatgtttt tatgtttttt cctcatttac tgtctggata tttggacaca gagttcaggc
    20881 ttcgtcgtca agtaagaact cttcgtaatc tgcaggcgat aacaattctg ctgaatcgtc
    20941 tggatttgaa agctctatac ggaccaccca gccgtcgtca tagcattctt cgttaattaa
    21001 ttcaggacta tccttcaact cagtattaac tgcgattata cgaccgctca caggcataaa
    21061 aagcgaagag accgctttta cagattcaac cgaaccgaat tcatcaccgg catcaatttc
    21121 gtcttcaatt tctggaagct ccgcaaaaac gatgtcaccc aatgcttttt gtgcatggtc
    21181 agtaataccg atttcggctt tatcgccctc tattcttatc cactcgtggt cgcgagtgta
    21241 gagattgtct tttggtattt cactcatcat tcctcttcaa attattttaa caggttgccc
    21301 gagaaagtat cgggtttcca tataaacaag ttcattgctg tacaacaata caatactaat
    21361 ttagccatag aacaatataa gtccagcaaa aaaagcactt cttttatttt acagacaatt
    21421 tgtaggaaaa aatcgatgga agatactaac aaagcaggat atgataatat tcatgaaaaa
    21481 acattattaa attttaacta ttgagaaaaa aaataaatgc aaaaatatta caagatattc
    21541 acattgatta tgatcactgg ttttttctgg gttactcctg cctttaccca aacctctgat
    21601 gatttggaac aaatgattcg tgcatctaaa acacgtgcag ctcttgttca aaatgttcaa
    21661 aaagctgttg tccatatcaa agtcgaaaaa attatgcgta gtcctgatgg ccgtgctctc
    21721 aacaatccaa gagacctcta caacgatgaa ttttttcgac gttttttccc ggaactccaa
    21781 cctccagata atcaacaacc aaaaaaacga gaacagccaa atcgtgaatt tcgacaaaaa
    21841 ggaatgggtt ccggttcaat aatagatgca gagggctaca ttctgaccaa ccaccatgtt
    21901 gtcggcgaag cagacgagat attggttgta ctatacaatg gagatgaaag gaaggcaaag
    21961 ctcgttggaa cagatcctga gtcggacatt gcagttgtga aaattgaagg taatggactt
    22021 ccggtgctgc caatgggaga ttcagataaa atacttgttg gtgaagatgt gattgcagta
    22081 gggaacccat tcggactaat ccagacagtc acttacggaa ttgtcagtgc caaaggtcga
    22141 tccaatgtag gcatcaatga atatgaaaat tttattcaaa cagatgctgc catcaatccc
    22201 ggaaatagcg gaggtccctt agtcagtctg cgtggtgaaa ttatcggagt caacagcgca
    22261 atttttagtc aaagcggcgg ctatcagggc attggttttg cggttccaat aaatatggct
    22321 cgcaaaatca tgcgggactt gatagaaaaa ggtacagttt ccaggggctg gctgggtgta
    22381 ggaattcagg atgtcagcca tgatctggct aaagctttta agctgaaaag tgccaaaggt
    22441 agcctgatta ctggaattat gcaggatact ccagctcaaa aagctggcat gcgcaaagga
    22501 gatgtcgtga tcagaatcaa tgacaaactg atccaaaaca gtaaccatct gcgcaacgaa
    22561 attgcaaacg ccggggcttt cgctgaaata gaaatggaac tgatcaggga tggaaaaacc
    22621 gttcttttga aactgaagct ggatgaacgt ccaaagaaaa ttgggaggat gaaaacctcg
    22681 ccggagccaa ctcaaactcc cgaacagtta gagctgttgg gcatgacagt tgaagaactc
    22741 acagaagaaa atgccaagca actggggatt gaacctggga ttggtgttgt gattagtaat
    22801 gttaaatccg gaagcccggc agaaaaatct gggttgctcc ctggaatgat tgtgcaggaa
    22861 gcagaacgtc aagctgtcag taacattgat actttcaaag aaattatcaa tgacattgat
    22921 cagggaaaag ggatactcct gctgatcaga accagtaacg gctcacgtta tatttttctt
    22981 caagaaaatt gaacacagta attacatgaa gtgaagaaga aaacaaaatt gcattttgat
    23041 cagttgcagc taaaattgca tcaaattatc cagcagaaaa ctatttcgga gaaaatggat
    23101 ttttattcac ttttggatga aatgtcagtt tattacagtc ttaccactga agaattactg
    23161 acacgtggat tccgtaaagc ttatcggcaa gctatagaag gcgtttaatt ttaatttcac
    23221 agtcgagttt gatgaattta tcttttctcc tggttggttt atcttcactg ttttttgcgt
    23281 tatggacagc atatggactg tacaggagat ataaagggac tcttccggaa cgtcccgcac
    23341 aacgaaaaag gtggtttggt gtatgtgcag acttttcaga aacggtgggc attccagtca
    23401 gcattgtcag gttttttgtg ttgttctaca caccagttgg attgggtcca gtattctatt
    23461 ttatctacta tctggtcatc cggaacaagg agcctatacc gctgaaatct ccagaacgca
    23521 atctgcaaat atcaaaaatt gaaagccact atttcggttc ttaggttacc atgacatccg
    23581 tctcgtacca gcattcagaa aaattcccgc tggcaggact acgttttctg gtaacccgtc
    23641 aggacagtac tgaatcttca ctttccggaa tgctagaatc tcagggggct tcagtactta
    23701 ctgcacaaat gactcaaata attccgacag aatcatggga gtcgtttgat gaaactgttc
    23761 agcaaatttc aaacattgac tgggctgttt tcaccagcag aaatggggta actcactgct
    23821 tgagtcgtct taatgatttg gaggtttctg ctcaacaatt attttcgtct atcaaaactg
    23881 cctgcgtcgg ccaggcaact gcttccgttc tgactgacaa tgggattact ccggaactgg
    23941 ttccagaaca ttttcaaagc gaggggctga ttgatgcttt taagcagcat gatttatttg
    24001 aaaaacgatg ttggttgatt caagctgaat ctccgcggaa aatcctgcgg gattccctgc
    24061 aaaaaatggg ggctcaaatc atcttaactc cagtttacag gaatgttcct gtcgtaggag
    24121 attatacttt tttactggct gaacttgagc agcaaaaatt ggattggatt ttgtttgcaa
    24181 gtccatctgc agttcaaaat tttcaccaaa tattaccatc tggcttttgg gagtcattgg
    24241 ctgcagaacc aaaaattgcc tgtctcggta aaattactgc aaaggcagtg cgggattttg
    24301 gttggagcgt tcaggctgca ccgaatattc aagactttga acatcttgtt caaagtcttt
    24361 gcgaaatcaa ctcacaaata tcagaaaaaa catgagtgat tctcaaaatc ttcttgttac
    24421 aatgcaagga ccagtttcag aggtggacgt tctggagcat tttacagtac atcttgagca
    24481 cagtgccttt tccatccgtg acattcatta cataaacagt caatcagcac tttcgctgtt
    24541 aatgcggctt gaacttgcgg actccgctga catagctgtt ctgaggaagg aattagacat
    24601 agcggcaaaa aaatataatc ttgagataca gattaatcca cttaacgaag cgcctgctgc
    24661 ttctcctgta tatcgttata ttttaaccct gctgagtcag caacttcgac cggagttact
    24721 aactaaactg ttccgtcaac tccgcgaaag gtcacttcat gtggccggaa tcagcccatt
    24781 ggaagcagat gatttgcatg tttttgaaat agaaattcat gctgaaaaac caatagatcg
    24841 tcagcaactg atgcatgggc tgctggaact taaatcagaa tatcaacttg atcttgccct
    24901 gcaggctgat gatctatttc ggcgcaacaa acgtttgatt tttctggatg cagacatgac
    24961 atttattcaa tgtgaaatga ttaatgatat gagtcatttg gcaggaaaag aagctgaggt
    25021 tgccgaaata acacatcgtg ctatggacgg gaaaataaat tttgacgatg cattgcggca
    25081 acgtgtagct ctacttaaag gagtccggct cagtgattta gaacgactta ttttgaatat
    25141 tccatacaca cccggagttg aacgcctcgt ttacattctc aaaacacttg gttataaaat
    25201 tggaattgtg agcggtggat ttaccagagt tatcgatcat ataaaacagc gtttcgatct
    25261 tgattacgga tttgccaaca cactggaagt gaaaaacgga gaattaaccg ggggcatctt
    25321 aggagacatc ctcgatggtc atcaaaaagg tcttatcctc cgggaagttg ctcttaaaga
    25381 aaatattctt ccggaacaag tgattgcagt cggtgatggc gccaacgatt tggaaatgct
    25441 ttccagtgcc agtttgggaa ttgctttcaa tgccaaaagt tttttgcggg aacgtgctgc
    25501 cggtagcctc tctcttccta accttgatgc actgctctat tttataggaa tttcccgtaa
    25561 tgaagtcaat gccttattta agcgagagcg tgtaaaatga atgagaacga atcaaaatat
    25621 tacagtattg aagaaatccg taaattccag gaaagaggag ttcaagttct ggattccagt
    25681 tctgttttca tttctcggga tgtagaacct gaaaatattc ttccgggatg catcatccat
    25741 ccttgttcca gaatctccgg agcaaaaaca caaattcatt cctcagcaca tattggagtg
    25801 agcggtcctg ctactataga aaatagttgg attggggaaa atgctattgt aggaaattta
    25861 ggacctgtaa ctctaaaaga tacagttgta ggccctcaaa ctatactcgg ctcaggagtt
    25921 gcggaaaatg cagttttttt gggtaaagaa acaatgatta atgattttac aaccggattc
    25981 ggattccgag tccggaaggg atcgctctat gaggaagact catcctctgc ccaacatact
    26041 gatacaaaaa tgacagtgct tttcccctgg accactttgg gaagtaatat taatttttgt
    26101 gatgccctga tttctggagg cactggaccg gaactgggtt attttagtga agttggcagc
    26161 gggagtattc attttaattt tactatcagg ggcgataaag caactgcgtc tttatttggt
    26221 gatgtctgcc agggtttatt tttggatcag gatcgtcttt ttattggagg aaacaatact
    26281 cttttgggtc cgatcaaggc agatttcggt gtgatgactg cggctggatc caggagcaat
    26341 ggtacactgt cgccgggatt gaattttgga cattctttac caaaaggcaa aattgactat
    26401 gaaccgcgaa tattttcagg agcagtgggg attgtaacaa aacaggttga tgtgcttgcg
    26461 gagttgactg cattattcca ctggtatcag caagtacgta ttggttgtat ttcccaaaca
    26521 acggaacaaa aattcgttta tgaatcagga ctgaatatag ttgaattaaa ttataaagaa
    26581 cgtcttttcc agttgggtcg ttatgtggaa gctttggaag gatctctaag catattcagc
    26641 ggcagcaata aaatgtcaaa aaaagaaacg gcagaacaaa gacaattgtt ggaaaaatgg
    26701 ccaaaaattc aacaacaatt ggcaacacca aaagcatttg aattgttgat tccggaatcc
    26761 ttaactaacg ctattgctcg aaaattagct gaaggtaaat tggattacac agttataatc
    26821 aaaggaatgg atatagaggg gaaacaaaaa gggaaggact ggctcaacac cattgcgaat
    26881 ggtgtcagga atattatcaa ttcggaaata gcaatggacg gataagttag atagaagcgc
    26941 atctggagaa gatgcgctca acaaattatg ctgccagttt tgtttttgct tcctcggcta
    27001 cttgggcaaa caatgtaggt tcttccatgg ctaaatatgc caaggttcta cgatccattt
    27061 caatgtccgc agcttttaat ccataaatga actgactgta gttcattcca tgtaaacgga
    27121 cagcagcatt gattctcact atccaaagtc gtctaaaatc ccgttttttc acttttctgt
    27181 cacgatagct agactctctt gaacgatcaa ctgcatcttt aactgtacgt aaaagacgac
    27241 tccgtcctcc acgatagcct ttagccgcct taaaaagaga tttttttcgc tttctggcgt
    27301 gaactgctct tttaactctc ataaaaatta atctcagttt atccgtttgg aagcatcata
    27361 tctacagagt tggagtctgc agcagatacc attgcttttt tgcgaagtac acgttttgta
    27421 tcctggttgc gcttacgcat accgtggcgt aaaaaagctc gtttccgctt gtaacctccg
    27481 cctccggttt ttttaaagcg ctttgcagct ccgcgatggg ttttcatttt catagcattc
    27541 ctactttctt tttttgggtg gagcaaggat catatgcatt gaacgtcctt cactactaat
    27601 tggctgctcc acagtaccgt cgtcaccaat ttcagcctga aatttttcta gtaattctac
    27661 acctaaatct ttatgggcta tttgacgtcc cataaatctg acaccaactt ttaccttgtc
    27721 gccatcttct agaaagcgaa gtgcattccg aagtttaaat tgaaaatcat gttcttcaat
    27781 tttaggcttc attttcacct ctttgagatg aactactttc tgattttgtc ttgcaacgtg
    27841 atttcgttta ctttgctggt atttgaattt accataatcc atcaaacggc agacaggagg
    27901 cttagctttt ggagatactt ctactaaatc caaattagca acacgtgcct tttcaagcgc
    27961 atcatctgtt gagataatac caacttgctc accgtctcca tcgataagcc gtatctcatt
    28021 gacacgaatc tgatcattaa ttcgtgttga ttcttcattt cttgtcggag gacgagacct
    28081 tcgtattcct atgagctttc tctctatttg aatgaaacac tggtaggcgc gggcgggatt
    28141 gaaccgccga cccctaccgt gtcgaggtag tgctctccca ctgagctacg cgcctggatt
    28201 attattaaca aactcaggat tgagcgtcgg caccgaccga ctcttccacc gggaaacccg
    28261 gcagtaccat aggcgcaagg ggactttact tctgtgttcg ggatggaaac aggtgtttct
    28321 cccctgccag aagcaccggc actcaagcct gagttcgtgt gtttggaggt tttggaatat
    28381 aagagcaagt gagagggtct ctttgggttt taatcttgta ttaagtcagg ccgcccggcc
    28441 aattagtacc agtcagcttc acatgttgcc atgcttccac ttccggccta tcaaacaggt
    28501 aatcttcctg cggcctttgg ggacctaatg gtccgggaga tcgctcttgg agccggcttc
    28561 ccgcttagat gctttcagcg gttatccgtt ccagacgtag ctacccggca atgctcctgg
    28621 cggaacaacc ggatcaccag aggtctgtcc accccggtcc tctcgtacta agggcagacc
    28681 tcctcagatc tcctgcgccc gcggcagata gggaccgaac tgtctcacga cgttctgaac
    28741 ccagctcgcg taccgcttta aatggcgaac agccataccc ttgggacctg cttcagcccc
    28801 aggatgcgat gagccgacat cgaggtgcca aaccgcgccg tcgatatgaa ctcttgggcg
    28861 cgattagcct gttatccccg gcgtaccttt tatccgttga gcgatggccc ttccacacgg
    28921 ggccaccgga tcactaagac ctactttcgt acctgctcga gctgtcgctc ttgcagtcaa
    28981 gctcccttgt gcctttgcac tctgcgccgg atttccaaac cggctgaggg aaccattgcg
    29041 cgcctccgtt actctttggg aggcgaccgc cccagtcaaa ctgcccacca gacagggtcc
    29101 ttgtcccgga taacgggact aagttagaac acagaaaaaa tcagggtggt atttcaaggg
    29161 tggctccaga gggactagcg cccccccttc gcagcctccc acctatccta cacaaatctt
    29221 tcccaagttc actgccaagc tgcagtaaag gtgcacgggg tctttccgtc ttgccgcggg
    29281 ttgacggtat cttcaccgcc atttcaattt cactgagaat cacattgaga cagcgcccag
    29341 atcgttacgc cattcgtgca ggtcggaact tacccgacaa ggaatttcgc taccttagga
    29401 ccgttatagt tacggccgcc gtttaccggg gcttcagttc ggagcttccc ccgctactaa
    29461 atgattcttt acgctttctt taactcgctc catcaaatat cgacgagtgc gaccatcatg
    29521 ttttaacatc ctctctctgt gacgagcact tttctcatcc agatatgctt catagtaaac
    29581 taatttccat attcgtcctc ttgttgactt ggtttgtcct tcattatgag cagttatcct
    29641 tgctcttaaa tcagaactat aaccaatgta gaactggtca ttatcactat gtatgatata
    29701 aacataatgc atttgcgttt ttaatcaaaa aaccatttag aagcggggta acccctccca
    29761 ttaaccttcc ggcaccgggc aggcgtcaca ccctatacat cat
//
LOCUS       DTVD01000090            2368 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_19215_length_2368_cov_299.047557, whole genome
            shotgun sequence.
ACCESSION   DTVD01000090 DTVD01000000
VERSION     DTVD01000090.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19215_length_2368_cov_299.047557"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /gene="gid"
                     /locus_tag="EYO68_03270"
     CDS             <1..165
                     /gene="gid"
                     /locus_tag="EYO68_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512415.1"
                     /note="TrmFO; Gid; glucose-inhibited division protein;
                     similar to GidA; the gene from Bacillus subtilis encodes a
                     tRNA-methyltransferase that utilizes folate as the carbon
                     donor and bound flavin as reductant; modifies tRNA at
                     position 54 (uridine) of the T-psi loop to form a
                     C5-methyluridine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIC06854.1"
                     /translation="LIRYITETDPSIFQPMNANFGLLDPPEKKMSKADRKKWYAERAL
                     HKAAEYANQV"
     gene            363..>2368
                     /locus_tag="EYO68_03275"
     CDS             363..>2368
                     /locus_tag="EYO68_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIC06855.1"
                     /translation="MFEKILIANRGEIACRIIRTCRRMGIRTVAVYSEADAGALHVQL
                     ADEACCIGKSAPAESYLRIGNVLDAAKKHDAQAIHPGYGFLSENAEFARRCSQSGIVF
                     IGPSAEAIEQMGAKDQAKCVMENAGVPVVPGYKGAEQSPEFLHAEAEKIGYPLMIKAV
                     LGGGGKGMRVVRKQTDFPEALESARNEARNAFGDDRVLLECFVSGPRHIEFQIFGDQH
                     GNVIHLFERDCSLQRRHQKIIEESPSPFLDEKLRSKMANAAVAAAKAVKYTGAGTVEF
                     IVGDDRKFFFMEMNTRLQVEHPVTEMITGLDLVEWQLRIAGGEKLPLSQKDVKQNGHA
                     IETRIYAENPETGFLPSIGTLQRLAFPGLNHNNSADNRATIRIDSGVEQGGEISIHYD
                     PLIAKLVVHADSRKQAIRTMQTALAQSGVLGVETNLGFLQSILQHPYFDAGRMDTLFV
                     DSNLESLLEENQDSVDWVCWGTAIACLLKDYAESETKAQESMEPCSPWNSLNNWRTGF
                     PEPHRVQLRNQQGTETEVMIIKENNSFRILNETEGILVKVSRHGDLLDLSWNTKGLEE
                     FGHHLLVLIHESQLLAVHEDGRTLFTRLDPLAYEQSEDVSDFRLSAPMPGNVIRVLVK
                     AGDEVSSGQPLLVMEAMKMEHTIVAPANGIVEEVLFQPGDLVQN"
BASE COUNT          677 a          472 c          607 g          612 t
ORIGIN      
        1 ttgatccgtt acattacaga aaccgatccc tcaatttttc agccgatgaa tgccaatttc
       61 ggtttgcttg atcctccgga aaaaaagatg agcaaagctg atcgaaaaaa atggtatgct
      121 gaacgggctc tacataaagc cgcggaatat gccaatcaag tttgaaaaga agttttagat
      181 gatgcagtcc taaaaagttg tcattcccgc gaaagcagga atccgggaac ttgtaagtat
      241 cttgaataat ggattcccgc tttcgcggga atgacaaaca agtgcgtgtt tgaatttttg
      301 tgagttcatt ttgaaaggtt tcttaataaa tcatgtacgc ataattctca tcaaagtgtt
      361 aaatgtttga aaaaatcctg atcgccaatc gtggtgaaat tgcctgccgc ataatccgga
      421 cttgccgcag aatgggcatc aggacagtcg ccgtttactc tgaagcggat gccggcgcgc
      481 tccatgttca gcttgcggat gaagcctgct gcatcggaaa atcagctccg gcagagtctt
      541 atttgcggat tggaaatgtg ctggatgctg ccaaaaaaca tgacgcacag gctattcatc
      601 cgggttatgg ctttttatcg gaaaatgcgg aatttgcccg gcggtgctct caatccggaa
      661 ttgtttttat tggtccgtca gcggaagcaa tagaacaaat gggagccaag gatcaggcca
      721 aatgtgtgat ggaaaacgcc ggagttccgg ttgttcctgg atataaaggt gccgaacaaa
      781 gtccggagtt cctgcatgca gaagcagaaa aaatcggcta tcccttaatg ataaaagcag
      841 ttctcggcgg cggaggtaaa ggaatgcggg tagtgcgcaa gcaaactgat tttccggaag
      901 cgcttgaatc tgcgcggaac gaagcccgga atgcctttgg tgatgatcgc gttttgctgg
      961 agtgcttcgt cagtgggccg cgtcatattg aatttcagat atttggtgat cagcatggga
     1021 atgtaatcca tctttttgaa cgcgattgct cactgcagcg caggcaccag aaaataattg
     1081 aagaatctcc ttcacctttt ctggatgaaa aactgcgttc aaaaatggca aacgcggctg
     1141 ttgctgcggc aaaagcagta aaatacacgg gggccggaac tgttgagttt attgtcggag
     1201 acgatcgcaa atttttcttc atggaaatga acacccggct gcaggtcgaa catccggtta
     1261 cggaaatgat taccggactt gatttggtgg agtggcagtt acggattgcc gggggagaaa
     1321 aacttccatt gagtcagaag gatgtcaaac aaaatggtca tgccatcgaa accagaattt
     1381 atgcggaaaa tccggaaacc ggatttttac caagtatcgg cactttgcag cgtttggctt
     1441 ttccaggatt gaaccataat aacagtgcgg acaacagagc aaccatcaga atagatagcg
     1501 gagttgaaca aggcggagaa atcagcatac actatgatcc gctgattgca aaactggttg
     1561 tacacgcaga ttcacgtaaa caagccattc gtactatgca gactgcatta gctcaatccg
     1621 gtgttcttgg agtggagaca aatcttggtt ttttgcagag cattttacag catccgtatt
     1681 ttgatgcagg aagaatggat acactgtttg tagattcaaa ccttgagtct ctgcttgagg
     1741 aaaatcagga ttcagtagac tgggtttgct ggggaaccgc gattgcctgt ttgctgaaag
     1801 attatgctga gtcagaaacg aaagcccagg agtcaatgga accttgttct ccctggaaca
     1861 gtctgaacaa ttggcggaca ggttttccgg aacctcacag ggtacagttg cgcaatcagc
     1921 agggtacgga aacagaagtc atgattatta aagaaaataa ttctttcagg attttaaacg
     1981 aaactgaggg aatattggtc aaagtttccc gtcatggtga tttgcttgat ctaagctgga
     2041 acaccaaagg tctggaagaa tttggacatc atttgctcgt gctgattcac gaatcgcaac
     2101 tgttggcagt tcatgaagac ggccgcacat tgttcaccag actggatcct ttagcttacg
     2161 aacagagtga agatgtcagc gattttcgac ttagcgcgcc gatgcccgga aacgtgatcc
     2221 gtgtgctggt taaagctgga gatgaagtaa gttccgggca gccgttatta gtgatggaag
     2281 ctatgaaaat ggaacacact attgttgctc ctgcaaatgg aattgtggaa gaagttcttt
     2341 ttcaacccgg agaccttgtg caaaatga
//
LOCUS       DTVD01000091            9408 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1932_length_9408_cov_350.411526, whole genome
            shotgun sequence.
ACCESSION   DTVD01000091 DTVD01000000
VERSION     DTVD01000091.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_9408_cov_350.411526"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            589..987
                     /locus_tag="EYO68_03280"
     CDS             589..987
                     /locus_tag="EYO68_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06856.1"
                     /translation="MLEDKTITRPVLRSFQENLIQRLGPDEGRALDVLGKDFFYLVDQ
                     LATKLFEQHEKDAPLLDLSESEFPWELQVFANQFLRECAQSSRQLTHFCQGLRKKLED
                     SEFDQEFWKILDEAYQHHFYVTDSKKHYLV"
     gene            1007..2023
                     /locus_tag="EYO68_03285"
     CDS             1007..2023
                     /locus_tag="EYO68_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIC06857.1"
                     /translation="MPPDSKEISKENDPSTITRPTTWTESWMASPGPVTVAEHLNLFI
                     KGLGMGTADIVPGVSGGTIAFITGIYHSLLTAISSINGKLFRQALRLRFKEALAELHL
                     RFLITLVSGIAVALISTAHLMHYLLTEHPVPTWSIFFGLITASILVVGHSINNRVTSF
                     PALALGTLLAYGITGLIPLHTPEEPWYIFFSGMIAICAMILPGLSGSFILLILGKYVF
                     ITAALRNPFAQDNLEIILIFIAGCIIGIIGFSRFLRYGLERWHDFTLALLTGFMIGAM
                     RKVWPWKITLESQIIRGKEHVLREENVWPLVNTELWISILLMGVGFASVILLDNISKN
                     RAED"
     gene            complement(2020..2799)
                     /locus_tag="EYO68_03290"
     CDS             complement(2020..2799)
                     /locus_tag="EYO68_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06858.1"
                     /translation="MSKALKINLNSQREIRQEGAQDPKSIPASSTLPVGRIKTDFFLP
                     ELNLDTVEDSTEKSSPQVQKVVSRINKFIENIADDYFLLGLHLISLHSLLKESKLNTE
                     QIRSWYAENINMPYSSAMQCRKVADVYSANPEQIGRYTASGAYLLSSCKTPEEREEIW
                     QEARGEKSAPSIRELRETLKRVRERQALEKLELEDVNTERENSVNKFRMTPDKIHESL
                     QTISHYCDSFVACEKPVEQAEMREVLLDSLRILLKDLEETV"
     gene            complement(2920..3696)
                     /locus_tag="EYO68_03295"
     CDS             complement(2920..3696)
                     /locus_tag="EYO68_03295"
                     /inference="COORDINATES: protein motif:HMM:PF01656.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIC06859.1"
                     /translation="MSIKIAVMTMKGGQAKTTLAINLASRIAKARREVLLIDSDIQAN
                     VTTTFKRDHHCNLANVILGRVSNFEFIELERHLSFFPSGGEAMVEASSYLSQTRFKDA
                     LFKNLLSSINHKIVIIDTAPSWSEVSRNILMYVDYVIVPVVTDYLGFSGCDQIMSYID
                     EFKESKLGQCKAEVLGIAITRSQPRTNLSQTITEGLRERWSNLLFKRVIEESVSIQEA
                     PAFQQNIFDYRKGRTRGAEMYDQLCREIMRRIIQRQKDSL"
     gene            complement(3848..5473)
                     /locus_tag="EYO68_03300"
     CDS             complement(3848..5473)
                     /locus_tag="EYO68_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HIC06860.1"
                     /translation="MSQIRLTSNLSTNNEQASIDPQKVAFAESDIRSKLLRLDQALYV
                     VQNGEAVGVCLAEDLKDSVAGTSMSLLSHVELLHVGQLGDPEFMRAYGINMAYMTGAM
                     ANGIASEELVIASGKVGLLSSFGAAGLVPPRIEEAINKIQQALPNGPYAFNLIHSPSE
                     DAIERGAVDLFLKHGVTTVEASAFLGLTPNIVRYRAAGLRLNAQNQVEIRNRVIAKIS
                     RTEVASKFMMPAPATILQQLLSEGSITEEQARLAANIPMADDITVEADSGGHTDNRPL
                     ILLLPTILALREQIQAKFGYNQPIRVGAAGGIGNPASALAAFMMGAAYVVTGSVNQSC
                     VEAAASAHSKSLLAQAEMTDVIMAPAADMFEMGVELQVLKRGTMFPMRAKKLYELYKN
                     YESIEAIPQEEREKLEQQVFRSSIDEIWRQTEEHFNARDPHQIERVKDNPKRKMALIF
                     RWYLGLSSRWSNFGEQGRESDYQIWAGPAMGAFNYWVKGSYLEEVSNRKTVEVAWHIM
                     TGAAYLYRLRLLQIQGIWLPPACEMYVPQPFSV"
     gene            complement(5503..7317)
                     /gene="lepA"
                     /locus_tag="EYO68_03305"
     CDS             complement(5503..7317)
                     /gene="lepA"
                     /locus_tag="EYO68_03305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_952319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HIC06861.1"
                     /translation="MKKTTPDPSLIRNFSIIAHIDHGKSTLADCLMKETGAVSEREAQ
                     EQLLDNMDIERERGITVKSQSVRLNYLAEDGKEYVLNLIDTPGHVDFTYEVSRSLAAC
                     EGALLVVDAAQGVEAQTLANVYMAIENDLEVLPVLNKIDLPTAEPERVCQEVEELIGL
                     DTSIAVRVSAKQGIGIQEVLQQVVDFVPPPAGNPDAPLRALIFDSWFDSYLGIVIMIR
                     IIDGKVRVGDKIRFMATAKEFEVVQLGVFTPFREECSELGLGEVGFLSASIKTIGHTR
                     IGDTVTTAENPSQEALAGYAEAKPMVFSGIFPVEGEQYEELKEALRKLALNDSSLSYE
                     LETSAALGFGFRCGFLGLLHMEITQERLEREFDLELITTAPSVVYQVHLKKGGVIRVD
                     NPSKLPSPVEIEFIEEPYIRGRVLTPQDYVGGIMKLAQDRRGVQTNMEYPSPNRVMLE
                     YELPLNEIVLDFYDQLKSISKGYASFDYELIEFRRGSLVKLDILLNGEIVDALSLIVP
                     RDKAPYRGRALAKKIKEFIPKQMYEVAIQAAIGAKIIARETQGAMRKDVTAKCYGGDI
                     TRKRKLLEKQKAGKKRMKRVGSVEVPQEAFMAVLKIDR"
     gene            7648..8580
                     /locus_tag="EYO68_03310"
     CDS             7648..8580
                     /locus_tag="EYO68_03310"
                     /inference="COORDINATES: protein motif:HMM:PF03279.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06862.1"
                     /translation="MSDSNSKLTPETFKFRHRLEFTLFNSLKRWGKGASKKTRRRGTI
                     LLDGLLYHGLRICRRIVAINLELAFPKFSTVERKQIARANYRWFARFCMDVLHMDAWK
                     GRTSEIAKCHNLEILDQALSEKKGVLLISGHFGNWEMIPPALAELGYPVTMYVGRQTN
                     PLTNELQNTARANFGVETIDKGKRATLQMGRALAANKIIAMLIDQNDNKSDLFVNFFE
                     KLASSSKGTAAFHLLRESPVVLVTCPYVGDHLEISFQRITFDLSGEQEKDTLAITQKI
                     TSAFENVIRKYPEQYFWMHRRWRARPQEDPEDIY"
     gene            8611..>9408
                     /locus_tag="EYO68_03315"
     CDS             8611..>9408
                     /locus_tag="EYO68_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIC06863.1"
                     /translation="MSSRKLTVKTENWSEEPFAISRGSDDNFDAVVVELEQNGCRGWG
                     EATPTEHYQESVSQSEMLIENIRSQLENGLSRTELQQTMPKGAARNAVDCALWDLEAK
                     LAGKRVWELPEIFLHLGKETLSEPENVTTVYSLGVDTPEKMGDVALKNAQRPILKIKL
                     TGEGDLERLEAIRKNAPETRLVVDANEAWTPEHFNRYVPELKQLGVEMIEQPFPADND
                     SVLKTLDHPIPVCADESCHDTADLDRLVGLYEFVNIKLDKTGGLTEAL"
BASE COUNT         2681 a         2124 c         1866 g         2737 t
ORIGIN      
        1 ctacaacttc cagttgggag gaagaggaaa gtcggcagaa taaatatgaa agtaaactga
       61 aacgctcaat tgtacgtcaa aaagtagcac agtttcttta agctacgaag tcttgattaa
      121 ggttttgatt aatgatcatg aactctgaat cttagttgcc aatgctttcc ggaatttctt
      181 ctgatctttt ttaataacta tattctcctc tgtagtcccg ccacagtatt caaggttcaa
      241 aagtcagttt ccttcaagtg taaaaaatta atatttccca ttccggtgca ctatattttg
      301 tgcaaaagcc cggctttccc cggtcaataa ttcatcttcc aataaaagaa ttcatgctgt
      361 cataattctt gcaggatgta tctaaatgcc ttttacaaat ctcattctgt tacccgacag
      421 tcgtgagttt tatcgtgtca tcccaaacgt atgtgaggga tcttatagga tgtcggtact
      481 ttatcaaatc tctccctttg gtcgagatga catagcgtag tcaataatat cagtatctta
      541 ctagaattaa tggctcacga attctgttac tactaggtta aaaaagtaat gctggaagac
      601 aaaacaatta caagaccggt cctcaggagt tttcaggaaa atttaatcca gagactcgga
      661 ccagacgaag gaagagcgct tgatgtactt ggtaaggact ttttttatct ggtagatcag
      721 cttgcaacga aactttttga acaacatgaa aaagacgctc ctctgttaga tttgagtgaa
      781 tcagaattcc cttgggaact tcaggttttc gcaaatcaat ttttacgaga atgtgctcaa
      841 tcttcccggc agttgactca tttttgtcag ggtttaagaa aaaaactcga ggattcagag
      901 tttgaccagg agttctggaa aatacttgat gaagcatacc agcatcattt ttacgttaca
      961 gacagtaaaa agcattatct tgtctgagta aaatattaat cacctcatgc ctcccgattc
     1021 caaagaaatt tcaaaagaaa acgatccctc aactataacc agaccaacta cgtggactga
     1081 atcatggatg gcctctcccg gccccgtaac tgttgcagaa catctaaatt tgtttattaa
     1141 aggtttggga atgggtactg ctgatattgt tccgggagtt tccggaggaa ctattgcatt
     1201 tataactggt atttatcaca gcctactgac agcaatttcc tcaattaatg gcaaattatt
     1261 ccggcaagca ctccgactgc gtttcaaaga agcattggca gaactccatc tgcgtttcct
     1321 gatcacactg gtttcaggaa ttgcggttgc cttaatcagt actgcacacc tgatgcacta
     1381 cctgctgaca gaacatccgg tacctacgtg gtccatcttt tttggtttga tcactgcttc
     1441 aattttggtg gtaggccatt ctatcaataa tcgtgttacc agtttccctg ccttagcttt
     1501 agggactctg ctggcctacg gaattaccgg gctgattccg ctgcatactc cggaagaacc
     1561 atggtatatt tttttcagcg gaatgattgc aatttgtgcc atgattcttc cgggattgag
     1621 tggttcattc attctgctga ttttaggaaa atatgttttt atcacagcag ctttaaggaa
     1681 tccgttcgca caggataatc tggaaatcat cctcattttc attgccggat gcataatcgg
     1741 aattatagga ttttcacgtt ttttacgtta cggactggag cgctggcatg atttcacatt
     1801 agccctcctc accggcttca tgattggtgc aatgcggaaa gtctggccct ggaaaattac
     1861 tttggaaagt caaataatcc ggggtaaaga acatgttctc cgggaagaaa acgtctggcc
     1921 gttagtcaat acggaattat ggatctcaat tctgctgatg ggagtcggtt tcgcttcagt
     1981 aatacttttg gataatattt ccaaaaaccg tgcagaggac taaactgttt cctccaaatc
     2041 ttttagcaag atccgcagag agtccagcag aacttcacgc atttcagcct gttccacagg
     2101 tttttcgcaa gcaacaaaac tatcacagta atgcgaaatt gtttgcagag actcatgaat
     2161 tttgtctgga gtcatccgga atttattcac agaattttct ctttcagtat ttacatcttc
     2221 cagctccagc ttctccaggg cctgtctttc acgtacccgc ttcagtgtct cccgcagttc
     2281 tctgatactc ggcgcagact tttctcctcg cgcttcctgc caaatctctt cacgttcttc
     2341 cggagtttta caactgctca aaagatatgc tccggaagca gtgtaccgtc cgatctgttc
     2401 cggatttgct gaataaacat ctgccacctt tcggcactgc atagcagaag agtagggcat
     2461 attaatattt tctgcatacc aggatcttat ttgctctgta ttgagtttag attccttgag
     2521 cagagaatgc agcgaaatta aatgcagccc aagtaaaaaa taatcatcag caatattttc
     2581 aataaattta tttatccggc ttaccacctt ctgcacctga ggagaacttt tttcggtaga
     2641 atcctctaca gtgtccaagt ttaactccgg aaggaaaaaa tcggttttga tccgtccaac
     2701 cggcagagtt gaacttgccg gaatcgattt aggatcctgt gcaccttcct gtcgaatttc
     2761 acgttgtgaa tttaaattaa tttttagtgc ttttgacatg ataatttaga atcagaaggc
     2821 ctggttttaa cctgatttgc gttcatcaag accgcaggaa taatcttata gaatatccaa
     2881 tagaaaatta aagcagatga acttgtagaa agtcaataat cacagagaat ccttctgtct
     2941 ctggatgatc cgacgcatta tttccctgca aagctggtcg tacatttctg ctccgcgggt
     3001 gcgacccttc ctgtaatcaa aaatattttg ctgaaatgca ggtgcttcct gaatggaaac
     3061 agattcttca atcacccgtt taaacagcaa attcgaccac ctttcccgaa gtccttcagt
     3121 tattgtttgc gaaaggtttg tccttggttg agagcgggta attgcaattc caagtacttc
     3181 tgctttgcat tgacccaact tgctttcctt gaattcatca atataggaca tgatttggtc
     3241 acaaccacta aacccaagat aatctgttac taccggaaca attacataat caacatacat
     3301 caaaatattg cggctgactt cactccaact gggtgccgta tctatgatca ctattttatg
     3361 attgattgat gacagcaggt ttttgaagag tgcatctttg aaacgggttt gacttagata
     3421 acttgaggcc tccaccattg cctccccccc agatggaaaa aatgacaggt gccgttccag
     3481 ctcgataaac tcaaagttac tgactcttcc caagattaca ttagccagat tacaatggtg
     3541 gtcacgtttg aaagtggtgg tgacatttgc ctgaatatct gaatcaatca agagtacttc
     3601 acgtctggct ttggcaatac gagaggctaa attgattgca agagtggtct tggcctgccc
     3661 acccttcatg gtcataacag caattttgat actcacacct cctctatttt ttcattctaa
     3721 ttgggaagaa ataaccgcta aaacggttat taaaatctat catgcctccg ataaagaaca
     3781 caagacatga aaagcgtcgg gcctctgcaa agctttccgg taaacttgta cttttagtcg
     3841 gttagtttca aacagaaaaa ggctgaggta catacatttc gcaggcaggt gggagccata
     3901 ttccctgaat ttgtaaaaga cgcagacgat acaaatatgc tgcaccagtc ataatgtgcc
     3961 aggccacttc caccgttttc cggttagata cttcttccaa atatgagccc ttaacccaat
     4021 aattgaatgc acccattgcc ggaccggccc aaatttggta atcagactct cgtccctgct
     4081 caccaaagtt tgaccagcgg gaagacaaac caagatacca acgaaaaatc agggccattt
     4141 tacgcttagg gttatccttc acacgttcaa tttgatgagg atctctggca ttgaaatgtt
     4201 cctccgtttg ccgccagatt tcatcaattg aactccggaa tacctgctgc tccagttttt
     4261 cccgctcttc ttgcggaatt gcttcgatgc tttcatagtt tttatatagt tcatacagtt
     4321 tttttgctct cattggaaac atggtaccac gcttaagtac ctgtaattcc acacccattt
     4381 caaacatatc cgctgccgga gccattatta catctgtcat ttctgcctgg gctaaaaggc
     4441 ttttactgtg cgcggaggcg gcagcttcca cacaggactg gttgactgaa ccagtcacta
     4501 cataagctgc acccatcata aaggcggcca gggcagaagc cggatttcca attccacccg
     4561 cagctcctac gcgaattggc tggttataac caaattttgc ctgtatttgt tctcgcaatg
     4621 ctaaaattgt cggaagaagt aaaatcagcg gacggttatc tgtatgtcct ccagaatcag
     4681 cttcaacagt gatgtcgtct gccattggaa tatttgctgc aagacgagcc tgttcttccg
     4741 tgatacttcc ctctgaaagt aattgttgaa gaattgttgc cggtgcaggc atcataaatt
     4801 ttgaagcaac ttctgtccgt gatattttag caataaccct gtttctaatc tcaacctgat
     4861 tttgtgcgtt gagacgtaaa ccagcagccc ggtaacgcac aatatttggt gtcagcccga
     4921 gaaatgccga agcctctact gttgtcacgc catgctttaa aaataaatca actgctccgc
     4981 gttctattgc atcttcgctg ggactatgga taagattgaa ggcatatggc ccatttggta
     5041 aagcctgctg aattttgttg attgcttctt caatacgggg cggcaccagg cctgcagctc
     5101 caaaactgct tagcaatcct acttttccgg aggcaattac cagctcctct gaagcaatac
     5161 cattagccat cgctccagtc atgtacgcca tgttaatacc gtaagcccgc ataaattccg
     5221 ggtcacccaa ctgccccaca tgcagaagtt ccacatgtga taaaagtgac atcgaagtgc
     5281 cggctacaga gtctttcagg tcttcagcaa ggcaaacacc aacagcctct ccattttgta
     5341 caacatataa tgcctggtcc aaacgtagca atttgctgcg gatatctgac tcagcaaaag
     5401 caaccttttg aggatcaatt gatgcttgtt cgttgtttgt tgaaaggttg gaagtgaggc
     5461 gaatttgact catattatat tattgtacat aattaaattt gattatcgat caattttcaa
     5521 aactgccata aatgcttcct gtggaacttc tactgaacct acccgcttca ttcgtttttt
     5581 cccggccttt tgtttttcca gtagtttcct cttgcgggta atatcaccac cataacattt
     5641 tgcagttaca tctttgcgca tcgcaccctg agtttcacgt gcaattattt ttgcacctat
     5701 tgctgcctgt attgcaactt catacatttg ttttggaata aactctttta ttttttttgc
     5761 cagtgcacgt ccacggtagg gagccttatc tcggggtacg attaatgaca aagcatcaac
     5821 tatttcacca ttcagcaaaa tatctaattt aactaatgaa cctctgcgaa attcaatcaa
     5881 ttcataatca aaagaagcat aaccctttga gatagatttt agctggtcat aaaaatccag
     5941 gacaatttca tttaaaggaa gttcatattc aagcatcact cgatttggtg aaggatactc
     6001 catgttggtc tgcacaccgc gccgatcctg ggcaagtttc ataattccgc ctacataatc
     6061 ctgcggagtc aggacgcgcc ctcgaatata cggttcttcg ataaattcaa tctcaactgg
     6121 tgacggcaat ttagatggat tatccacccg gataactcca ccttttttga gatgaacctg
     6181 ataaacaaca cttggagcag tggtgataag ttccaaatca aattcccgtt ccagacgttc
     6241 ttgggtaatc tccatatgca gcagtcccag aaaaccgcag cgaaaaccga aaccaagtgc
     6301 cgcagaagtt tctaattcat aactcaatga agaatcattc agagccagtt tacgcaaagc
     6361 ttctttaagt tcttcatatt gttctccttc caccggaaag ataccgctaa acaccatcgg
     6421 ctttgcttct gcataaccag caagcgcttc ctgactggga ttttctgcag ttgtgacagt
     6481 atctccaatt ctggtgtgtc caatcgtctt gatggaggca cttaaaaaac cgacttcccc
     6541 caacccaagt tcagagcact cctcacgaaa aggagtaaat actcccagct gaactacctc
     6601 aaactcttta gcagttgcca taaagcgaat tttatcacca acccgcactt taccatctat
     6661 gatccggatc attatgacaa ttccaagata ggaatcaaac catgaatcga aaatcaatgc
     6721 cctcaatggc gcgtctggat tgcctgctgg aggcggcaca aaatccacta cctgctggag
     6781 gacttcctga atgccaattc cctgtttagc acttactctg acagcaatgc tggtatccaa
     6841 gcctataagc tcttcaactt cttgacaaac tctttccggt tctgctgtag gcagatcaat
     6901 tttgttcaaa actggcagca cctccaaatc gttttcaatg gccatataaa cattggccaa
     6961 ggtctgcgcc tccacgccct gtgctgcatc aacaaccagt aatgctcctt cacatgctgc
     7021 caaagaccgg gagacttcat aagtaaaatc aacatgtccg ggagtatcaa tcaggttcag
     7081 gacatattct tttccgtctt ctgccaggta attcaagcgc acactctgcg actttactgt
     7141 aatcccgcgc tcacgctcaa tatccatgtt atccagcagt tgctcttgcg cttcacgttc
     7201 tgaaactgca cctgtctctt tcattaaaca gtcagccaag gtggattttc cgtgatcgat
     7261 atgagcaata attgagaagt tccggataag gctgggatca ggtgttgttt tcttcatgta
     7321 ttattataca aatattaata ttaccatcca gtgaggacat gatgattttc caattgttta
     7381 atagttcgca actcttctga ctgattgaca gttttcgatg tgcagacaat cgacagtcat
     7441 aataaattat ttatatttct gcttgtaata gtgacaaact cttagatata aaatgacaca
     7501 cttgtatcaa aatatcgagt ccagactgct ttctgcatta gtttaagaag cattaggtac
     7561 aggattgaaa aagagtattc tggttttcaa gttgggctga atctgttatt gttttgttaa
     7621 agattcacga aatctctcaa tctgaaaatg tctgatagca acagcaaact tacaccagaa
     7681 acattcaaat ttcgccaccg ccttgaattt acccttttca acagcttaaa acgttggggc
     7741 aaaggtgcat caaaaaaaac gcggcggcgt ggtacaatac tgcttgatgg cctactttat
     7801 catggtttgc gcatttgcag gcgtatagtc gcaatcaatc tggagctcgc ttttccaaaa
     7861 ttctcaactg tagaacggaa acaaattgca cgtgcaaact accgatggtt tgcccgtttt
     7921 tgtatggacg ttttgcacat ggatgcttgg aaaggtcgta cctcagaaat cgcaaaatgt
     7981 cataatctgg aaatactgga tcaagcttta tcagaaaaaa agggcgtgct tctgatcagc
     8041 ggacactttg gcaattggga aatgattcca ccggccctgg cagaactggg gtaccccgtt
     8101 accatgtatg tcggtcggca aaccaatccg cttaccaacg aacttcaaaa tactgcacgt
     8161 gccaattttg gagttgaaac tattgataaa ggtaaaagag ccaccctgca aatggggaga
     8221 gcacttgcgg ccaataaaat cattgcaatg ctgatcgatc aaaacgacaa taaatctgat
     8281 ctttttgtaa acttttttga aaaacttgct tcctccagca aaggaacagc cgcctttcat
     8341 ttgttgcggg aaagtccggt tgtactagta acatgtcctt atgtcggtga tcatttagaa
     8401 atatcatttc agcgcattac ttttgatctt tctggagaac aggaaaaaga tacgctggca
     8461 ataacccaaa aaatcacttc tgctttcgaa aatgtgatcc gaaaatatcc tgaacaatat
     8521 ttctggatgc accgtcgttg gcgcgcacgt cctcaggaag atccggaaga tatatattaa
     8581 catcaacttt taaagaacaa aaaatattct atgtctagcc gaaaacttac cgtcaaaact
     8641 gaaaactgga gtgaggaacc ttttgcaatt tcaagaggaa gtgacgataa tttcgatgca
     8701 gttgtcgttg aattggaaca aaacggctgc agaggatggg gtgaagccac tccgactgaa
     8761 cactaccaag agtctgtctc tcagtcagaa atgctgattg aaaatattcg aagccaactg
     8821 gaaaacggtt taagccgcac agaactgcag caaactatgc caaaaggcgc ggccaggaat
     8881 gcagtggatt gcgcactctg ggatttggaa gcaaaacttg ccggaaaaag ggtctgggaa
     8941 ctgccggaaa tttttctgca tttgggaaag gaaactcttt cggaaccaga aaacgtaaca
     9001 actgtgtatt cgctcggtgt agataccccg gaaaaaatgg gtgacgtcgc gctgaaaaat
     9061 gcccaaagac caattttaaa aattaaactc accggcgaag gcgacttgga acgactggaa
     9121 gcaatcagaa aaaatgctcc ggaaacacgt ttggtggttg atgcgaatga ggcttggact
     9181 ccggaacatt ttaatcgtta tgttccagaa ttaaagcagc ttggagtaga aatgattgaa
     9241 cagccttttc ccgcagataa cgacagtgtt ttgaaaacac tggaccatcc cattccggtc
     9301 tgtgccgatg aatcctgtca cgacaccgct gaccttgatc gtttagtggg actctatgaa
     9361 ttcgttaata ttaaactgga taaaaccgga ggactcaccg aagcgctg
//
LOCUS       DTVD01000092            9357 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_1945_length_9357_cov_293.703612, whole genome
            shotgun sequence.
ACCESSION   DTVD01000092 DTVD01000000
VERSION     DTVD01000092.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_9357_cov_293.703612"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2566)
                     /gene="xdh"
                     /locus_tag="EYO68_03320"
     CDS             complement(<1..2566)
                     /gene="xdh"
                     /locus_tag="EYO68_03320"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HIC06864.1"
                     /translation="MEKIKLKEQTMEFKLNGTTINYEGDPELSLMTYLRDVEDIISPK
                     DGCAPEGVCGCCTVLLDGNVLKACIAPMRRIAGKEVITMEGLDPGKKETVINAFAIEG
                     GLQCGFCTPGIIMKVWPLLNQGFVTEKEINKALNSNLCRCTGYKKVTKSCLSAAEALR
                     NNAKIELPQSSGKVGESLPKYDSLRLATGEAPYVADLKFEGMVHGALKFSNHPRAKVL
                     KINTSVAEKLDGVLRVFTAEDIPGERHTGLIVPDWPLMVKRGETIRYVGDVLAGVVAE
                     TEQIAREAVALIEVEYEILTPVTDPFAALSAESPKIHESGNLLSNTEIERGDANQAEK
                     ESAFVTKGTYKTQRIEHAFLEIECCVVKPMEDGVEVFSQSQGVYEDRNSISKILGLPS
                     EKVVVVLMPNGGGFGGKEDISVQGHAALYSHLLQVPVRVALTRPESLCMHPKRHPMIM
                     EMSLGCDENGKLTFVEADIIGDTGAYASVGMKVLERAAGHATSAYSIPIVKLRSRSVY
                     TNNIPCGAMRGFGVNQINFAIESCIDELCVQGGFDRWQIRYDNALQPGDRTSTGQKIT
                     SGIGIQKTLIAVKEQFQNAKFAGIACGMKNTGIGNGVQDESKVKVVIDSPTEITIHHG
                     WTEMGQGVFTMAVQFLCEATGVNPNFISVKVDTSKDTPCGMTTASRGTSLIGNSVLDA
                     GNKLKADLETHSLQELVGREYVGEWVCDWTTAIEADADEPQTHYSYSYATQVVTLDDS
                     GKVENVFAAHDAGRIVNPILFEGQLEGSVHMGLGYALSEKFDMEEGRPVHTKLGKLGM
                     IRASATPNIVVIGIEEPDEFGPCGAKGVGEIGLVPTASAVANAFFQFNGERQYELPL"
     gene            complement(2572..4665)
                     /locus_tag="EYO68_03325"
     CDS             complement(2572..4665)
                     /locus_tag="EYO68_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HIC06865.1"
                     /translation="MAELIPHPFGALVTRMFTELETEKSIFDYPQKKFFIGQSGRDYS
                     VKFHGKNSSSPLGPASGPQTQMAQNLVLSWLGGSRIMELKTVQILDELEIPRPCIDMQ
                     TVGYNVEWSQELRVEQSLHEYVKGAMLIEILRASGKLDLAENFGDVLYDMSVGYDLKG
                     IQSDKVRRFIEGMLDASEVVEHYRKQIPEQYRQFRNLDFQTKLSDTLTLSTFHGCPPE
                     EIEKIIDYLFREHGLNCIIKLNPTLLGKDQVRHLLNGIMGYADVHVPDEAFENDATWE
                     QAQGFVERLGLTAKTLGLGFGVKFNNTLIVENHRNFFPDTEKVMYLSGTPLHVLGINL
                     VKQFREIFGDQFPISFSAGIDKTNFADTVALGLTPITVCSDLLKVGGYSRSSAYYKEL
                     NSRMDNLGVSDIESYILKAYGNAEQALENIGLGVGNVSGPDVPLADACRKTLANGGEL
                     RKVAGSEAPVANETFEKWLSETKLLNTKTYVDEVTTNARYGIEQNSKPPRKVGTMLEL
                     FDCLTCDKCIPVCPNDANFALKIPPGETEILEFETNNSGWAVTGRKTLKLEKKYQIAN
                     FADFCNECGNCDIFCPEDGGPFVLKPRFFGSLESFQSFTNHDGFYIEDEGTERCAPKV
                     FARFDGKEYRVSETGNTVNYSGPDFDIQFSKNDLENTISGEGKSSVSFLNYEIMQMMR
                     SAISATGSGSYVSAT"
     gene            complement(4733..6046)
                     /locus_tag="EYO68_03330"
     CDS             complement(4733..6046)
                     /locus_tag="EYO68_03330"
                     /inference="COORDINATES: protein motif:HMM:PF01979.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06866.1"
                     /translation="MKKTLLKNLHVINPCSDPAFIENAFVAISGETIESVDSSEPSGT
                     FDEVFDLDGKVALPGMINAHHHLYSALAVGMPLPKGNPTNFTEILQEVWWKMDLALDR
                     DSTQACFESGLLDSLKAGTTTFIDHHCSPSFIEGSLSLLAETGEKFGLNSSVAFEITD
                     RNGTEKFEASLQENINAVRKFSDHPNVHPLIGLHASFTLSEDSLRKIRTALDSLKSWG
                     IHIHVSEDKADEEDALKKGYGSVLERLDKFDLLNENALIIHGLHIKDSDVDLLQKHKA
                     QIVHNPSSNANNRVGMSSNNTINQLKSGLGTDGMQGSMLREGKEGMLIRSSHLGGGED
                     SANYLQLLFENNPTIASKLFGKKLGYILPEYQADLAIFDYAPRTPISDATIFGHVFFG
                     LSGLPCDVMTRGEFRIRDYQLQNFSEQEIKANAVTQAKKLWVHFS"
     gene            complement(6131..7180)
                     /gene="lpxK"
                     /locus_tag="EYO68_03335"
     CDS             complement(6131..7180)
                     /gene="lpxK"
                     /locus_tag="EYO68_03335"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:PF02606.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIC06867.1"
                     /translation="MNFSSKLFEPFLLVTSQIYKAIVLLRLQCYKNGWLKTHQPETPV
                     ISVGNLTVGGTGKTPVVDFLVKEIQNQKKRPAILSRGYRRKNGSTQQRFRFCEDSTID
                     PDIIGDEPFLLALRNPEVPVYVGSSRITAAHSAEIRDHPDVLILDDAYQHLAIRRDLN
                     ILLIDAERGLGNGSLLPLGILREPENQWVRADLIIITKTNLAASDSVMQMLKNELKVN
                     CPVFKFSFEPQRLSRLDGQAQLNVLQLKGKRLLLTSGIAQPAGFGLLLEQQGGNVIRK
                     LEFQDHHDYVFKDVQKILHEQEKLQPDYIVTTEKDAVKLRKFPELLEKLWILEIGVHP
                     EDSWNNYFTEFLNNL"
     gene            complement(7177..9069)
                     /locus_tag="EYO68_03340"
     CDS             complement(7177..9069)
                     /locus_tag="EYO68_03340"
                     /inference="COORDINATES: protein motif:HMM:PF00263.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06868.1"
                     /translation="MKKWLLCIMIFFITGFSELCAESGIVWKRTDKSGVVLSTVVSAT
                     LPVMLEKLFQEFALYPEKIKVDSSVTGIPEVKEVRGHNPETVLSLLLLRHDLTLVSQQ
                     DRHLIISRKDAWRHRPFRKILNSPVFLEKILRELSQVGQIALHFDSDKIQGRKVQRNL
                     NYSSIEDAIFDLASHQNAVVVYYPRLHVLEWTQDPPLVTKSVVLSSASQKQVQSFLTE
                     IRKELEEIRLVHDSYPTEKSLILRGSEESVVLTVQLIEQWESGLQKVSSADVEPFPAS
                     PAVESEGIEPKLIPETRIVRLKLKYLSVGPQTVQSNGQPLTIPGVEESLRKALQQRLE
                     DEAEIPPLRPQIIPDLLGNSLILEGSKTHVDWMEKLVQVWDSPLPLIRIEAHLFETSE
                     THSRQLGLEFRGKSVSTDGTVSITDQGPFTAGLALGPAAASQALRVDAVLRLLQSEGK
                     GRMLSRPVVVTLNNIEAEMNSGSVLHIKISTEKTSRLQELKTGITLRVTPRLIEDSDN
                     HSNDRIWLKIYAETSNPIEGSSIDGIPPINTQRAQTQVFVKDGQPFLLGGLIKNRTGE
                     SQSGVPFFKDLPFLGTFFRTSGSNNSFDHVMVFVTPTRVFADEIQELPEFSELEQIKK
                     TLEIKP"
BASE COUNT         2514 a         2205 c         1843 g         2795 t
ORIGIN      
        1 tcaatggcaa ctcatattga cgttcgccgt tgaattgaaa aaaggcgttt gctactgcag
       61 aagcagttgg tacaaggcca atttcgccaa caccctttgc gccacaggga ccgaattcat
      121 ccggttcctc tatgccgata accacgatat taggagtggc ggacgcacga atcatcccaa
      181 gttttccaag ctttgtgtgt acagggcggc cttcttccat atcaaatttt tcagaaagcg
      241 catatcccaa tcccatgtga accgaacctt caagctgtcc ttcaaataaa attggattaa
      301 caatcctgcc ggcatcgtgt gctgcaaaaa cgttttccac ttttccggag tcatccagtg
      361 ttacaacttg cgtggcataa ctgtaagaat aatgcgtttg tggttcatct gcatctgcct
      421 caatcgccgt tgtccagtca cagacccatt caccgacgta ttcacgcccc accaattctt
      481 gcagtgagtg tgtttccaaa tcagctttca atttattacc agcatccaaa acgctgtttc
      541 caatgagaga ggtaccacgt gaagcagttg tcattccaca gggagtgtct tttgaagtat
      601 ctaccttgac tgaaatgaaa tttggattga cgccggttgc ttcacataaa aactgaactg
      661 ccatcgtaaa aactccctgt cccatttccg tccagccgtg gtggatcgta atttcagttg
      721 gtgaatcaat caccactttg actttgctct cgtcctggac tccgttgccg attccggtat
      781 ttttcatacc acaggcaatt ccggcgaatt tagcgttttg aaactgttct ttcactgcaa
      841 ttaatgtttt ttgaataccg attccggaag ttattttttg accggtcgat gtccggtcac
      901 cgggctgaag ggcattgtca tatcgtattt gccagcggtc aaacccacct tgtacgcaca
      961 gttcatcaat acagctttca attgcaaaat tgatttggtt tacaccaaat ccacgcatag
     1021 ctccgcatgg aatattattg gtgtaaacag atcggctcct gagctttaca attggaattg
     1081 agtaagcact ggttgcgtgc ccggccgccc gttccagcac tttcatacca acggacgcat
     1141 aagctcctgt atcgccgatg atgtctgctt caacaaaagt caacttgccg ttttcgtcac
     1201 agccaagtga catttccata atcattggat gccgctttgg atgcatacaa agtgattccg
     1261 ggcgagtcag ggcgactctt accgggactt gtaacaaatg ggaatacaaa gctgcgtgac
     1321 cttgcaccga gatgtcctcc ttgccgccaa acccgcctcc attcggcatc aaaacgacga
     1381 caaccttttc tgatggtagt cccaatattt tactgatgct gttccggtcc tcatagacac
     1441 cctgactctg agaaaaaacc tcaactccat cttccattgg ttttactaca cagcattcaa
     1501 tttccaaaaa agcatgttcg atgcgttgtg ttttgtaagt accttttgtc acaaaagcac
     1561 tttctttttc tgcctgattt gcgtcgcctc gctcaatctc tgtatttgac aacaggttcc
     1621 cgctttcatg tatttttgga ctttcagctg acaatgctgc aaaaggatcg gtaaccggtg
     1681 ttaaaatctc atattccacc tctatcagag caacagcctc gcgggcaatc tgttcggttt
     1741 ccgctaccac ccctgccagc acatccccga cgtagcgtat tgtttctcca cgtttcacca
     1801 tcaacggcca gtccggaaca atcaaaccgg tgtggcgctc tcccggaata tcttcggcag
     1861 taaatactcg caatactcca tccaattttt ctgcaacgga agtgttgatt ttcagaacct
     1921 ttgctctggg atggttactg aatttcaagg caccgtgtac cataccttca aatttcagat
     1981 ccgcgacgta aggcgcttcg cctgttgcca aacggaggga gtcatatttt ggcaggcttt
     2041 caccgacttt tccgctgctt tgcggcagct cgattttggc attattccgc aaagcctctg
     2101 ccgcagatag acaggatttg gtcacttttt tatagccggt acagcggcaa agattactgt
     2161 tcagggcctt gttgatttct ttctctgtca caaatccctg attcaacaag ggccagactt
     2221 tcatgatgat tcccggtgtg cagaatccgc actgcagacc tccttctatg gcaaaggcat
     2281 tgatcacagt ttcttttttt cccggatcca gtccctccat cgtgatgact tcttttcctg
     2341 caatacgtct catcggtgca atacaggctt tcagtacatt cccgtccagc aagacagtac
     2401 agcatccaca aactccttcc ggagcacaac cgtctttggg tgagatgata tcctcaacat
     2461 cacgtaaata tgtcatcaaa gacaattctg gatcaccttc gtaattaatg gtggtaccgt
     2521 taagtttgaa ttccatggtc tgctctttta gttttatttt ttccatttaa attaagtggc
     2581 actaacataa gaaccacttc cggtggctga aatagcgctt ctcatcattt gcattatctc
     2641 ataatttaag aaacttacac tactttttcc ctcaccggaa atggtgtttt caagatcgtt
     2701 tttggaaaat tgaatgtcaa aatccggtcc ggaataatta acagtatttc cagtttcaga
     2761 aacacgatat tctttgccgt caaaacgggc aaatacctta ggggcacagc gctctgtgcc
     2821 ctcatcctca atataaaatc catcatgatt tgtaaatgat tgaaaagact ccaagcttcc
     2881 aaaaaaacga ggcttaagta caaacggacc cccgtcttct gggcagaaaa tatcgcagtt
     2941 tccgcactca ttgcaaaaat ctgcaaagtt cgcaatttga tattttttct ctaattttaa
     3001 ggttttcctg ccagtaaccg cccatccgga attattagtt tcgaattcca gaatttctgt
     3061 ttcacccggt ggaattttca gtgcaaaatt agcatcgttc ggacaaacag gaatgcactt
     3121 gtcgcaggtg aggcagtcga ataattcaag catggtaccg actttacggg gcggttttga
     3181 gttttgctct attccataac gtgcgttagt agtcacttcg tcaacgtatg tttttgtgtt
     3241 caagagcttg gtttcggaaa gccatttttc aaaagtttca ttcgccaccg gagcttccga
     3301 tccggcaact ttcctgagtt caccgccgtt tgccagtgtt ttgcgacaag catccgccag
     3361 cggaacatcc ggtccggaaa catttccaac tcccaaacca atattttcca gcgcttgttc
     3421 agcgtttccg taagctttga gaatgtaact ttcaatgtcg ctaacaccca gattgtccat
     3481 gcgcgaattc agctctttgt aataagcact gcttctgctg tagccgccaa ctttaagcaa
     3541 atcactgcaa acagtgattg gtgtcaatcc taaagcaacg gtatctgcaa agttggtttt
     3601 gtcgattccg gcagaaaagg aaataggaaa ttggtcgcca aaaatttctc tgaactgctt
     3661 aaccaaattg atgcctaaaa cgtgtaaagg cgttccggaa aggtacatta ctttttctgt
     3721 gtccgggaag aaattccggt ggttttccac aatcagcgtg ttattgaatt tgactccaaa
     3781 acccaatccg agcgtttttg ccgttaaacc caggcgctca acaaaaccct gtgcctgttc
     3841 ccaggttgca tcattttcaa aggcttcatc cggaacatgg acatcggcgt aacccatgat
     3901 tccatttaat aaatgacgca cctgatcttt tcctaacaga gttggattga gtttgatgat
     3961 acaattcaat ccatgttccc gaaaaagata atcaattatt ttttcaattt cttccggagg
     4021 acatccgtga aaagtactta aagtcagtgt gtcggaaagt ttcgtctgga aatccaggtt
     4081 ccggaattga cgatactgtt ccggaatttg ttttcggtaa tgctccacga cttcggaagc
     4141 atccagcata ccctcaataa aacggcgcac tttgtcagat tgaatcccct tcaaatcata
     4201 accgacactc atgtcataca gcacgtcgcc gaaattttcc gccaaatcca gctttccgga
     4261 tgctcgcaat atctcaatca acattgcgcc tttgacatat tcgtgtagcg actgctcaac
     4321 ccgcaactcc tgcgaccatt ccacattgta accgaccgtt tgcatatcaa tgcaagggcg
     4381 cggaatttcc aattcatcca ggatttgcac tgttttgagt tccataattc ttgagccacc
     4441 gagccaagac aagactaaat tttgtgccat ttgcgtttgc ggaccggaag ccgggccgag
     4501 tggcgaagaa ctattttttc cgtgaaattt aacggagtaa tctcgtccgg attgtccgat
     4561 aaaaaatttc ttttgcggat aatcgaaaat tgatttttct gtttccaatt cggtgaacat
     4621 ccgagtcacg agtgcaccga atggatgtgg gatgagttct gccataaaaa agtttcaaag
     4681 tttcaaagtt tcagagtggt agccccgtgc ctccgtgccg gggttaatat tttcatgaaa
     4741 aatgtaccca tagttttttt gcttgcgtaa cggcattggc cttgatttct tgttccgaaa
     4801 aattttgcaa ttgataatca cgaatccgga attcgcctcg tgtcatcaca tcacacggca
     4861 aaccgctcaa accaaaaaaa acatgtccga aaatggttgc atcagaaatc ggcgttcttg
     4921 gagcataatc aaaaatcgcc aaatctgctt gatattccgg taaaatgtaa cccagttttt
     4981 tcccaaataa tttggaggca attgttggat tgttttcaaa taaaagctgc agataatttg
     5041 cactatcttc tccgccacct aaatgtgaac tgcgaatcaa catgccttct tttccttcgc
     5101 gtaacatact gccttgcatt ccgtctgttc caagtccaga cttgagttga ttgatagtat
     5161 tgtttgatga cataccgaca cggttgttgg catttgagga cgggttgtga acgatttgcg
     5221 ctttgtgttt ttggagcaaa tccacatccg aatcttttat gtgcagtcca tgaataatca
     5281 gcgcattttc attaagtaaa tcaaatttat ccagccgttc caaaacggat ccataacctt
     5341 tttttagggc atcttcttcg tctgctttgt cttctgaaac atgaatgtga atgccccatg
     5401 attttaacga atcaagggct gtccggattt ttcttaaaga atcttcggac aaagtaaaag
     5461 aagcatgcaa accaatcagc ggatggacat ttggatgatc agaaaatttc cggacagcgt
     5521 taatattttc ctgcaaactg gcctcgaatt tttctgtacc gttacggtcg gtgatttcaa
     5581 aagcaacgct ggaattcaga ccaaatttct ctccggtttc tgccagcaaa gacaacgacc
     5641 cttcgataaa actgggactg caatgatggt cgatgaacgt tgttgttccg gctttgaggc
     5701 tgtccagcaa tccggattca aaacaggctt gtgtggaatc acgatccaaa gcaagatcca
     5761 ttttccacca gacttcttgc aaaatttcag taaagttggt cggatttcct ttgggcaaag
     5821 gcattcccac tgccagcgca gaatatagat gatggtgcgc attgatcatg ccgggcagtg
     5881 ctacttttcc gtccaaatca aaaacctcat cgaaggttcc ggacggctca ctgctgtcca
     5941 cactttcaat cgtttcaccg gaaatagcaa caaaagcatt ttcgataaag gccggatccg
     6001 agcaaggatt gatgacatgt aaatttttca gcagtgtttt tttcatgagg atctcattac
     6061 atatgttact cagtgacggg ctattatatc gtatctgacg gttccaatca aatttttggc
     6121 tccattttcg ttataaatta tttagaaatt ctgtaaaata attattccat gaatcttccg
     6181 gatgtacacc gatttctaaa atccaaagtt tttcgagaag ttcaggaaat tttctgagtt
     6241 ttacagcatc tttttctgta gtcacaatat aatcaggttg cagcttttcc tgttcatgca
     6301 gtatcttttg tacatcttta aaaacataat catggtgatc ctggaattca agcttccgga
     6361 taacatttcc cccttgttgt tccagcaaaa gtccaaatcc tgcgggttga gcaattccgg
     6421 aagtgagcag cagtcgcttt ccttttaact ggagaacgtt cagttgcgct tgcccgtcca
     6481 gtcgggaaag acgctgaggc tcaaaactga atttaaaaac aggacagttg actttcagct
     6541 catttttgag catttgcatt actgaatctg aagcagccaa attagttttt gtaataatga
     6601 ttaaatccgc acgtacccat tgattctccg gttcacgtaa tattcctagt ggaagtaagg
     6661 aaccgtttcc taatccacgc tctgcatcaa tcaagagtat attcagatca cgccggatag
     6721 ccaaatgttg gtatgcatca tccaatatca gaacatcagg atgatcccga atttctgccg
     6781 aatgagctgc agtgatccgg gaactgccca cataaaccgg aacttcagga ttccgcaaag
     6841 caagtaaaaa cggttcgtca ccaataatat ccggatcaat tgtactgtct tcacaaaaac
     6901 gaaaccgttg ctgagttgat ccattctttc tcctgtagcc tcggcttaga atggctggac
     6961 gctttttttg gttctggatt tcctttacca gaaaatcaac aaccggagtt tttcctgttc
     7021 cacctactgt taaatttcca actgaaataa ccggagtttc aggctgatgt gtttttaacc
     7081 agccattttt gtagcattgt agccgcaaaa gaactatggc tttgtaaatc tgtgaggtta
     7141 ccaaaaggaa cggttcaaat aatttacttg aaaaattcat ggttttattt ctaatgtttt
     7201 cttgatctgc tccagttcag aaaattctgg taattcctga atttcatctg caaatacacg
     7261 agttggagtc acaaacacca tcacatgatc gaaactgtta tttgaaccac ttgtccggaa
     7321 gaacgtaccc aaaaaaggaa gatctttaaa aaacggtact ccggactggc tttctcctgt
     7381 tctgtttttg atcaatccac caagcaaaaa aggttgacca tccttcacaa aaacttgagt
     7441 ttgtgcacgc tgagtattga tcggtggtat tccgtcaatg ctggaacctt ctattggatt
     7501 actggtttcc gcatatatct taagccaaat ccggtcatta ctgtggttat cactgtcctc
     7561 aatcaagcgt ggtgtcaccc tgagtgtaat gccagttttc aattcctgca gacggctcgt
     7621 tttttcagtt gagattttaa tatgcagaac cgaaccacta ttcatttctg cttcaatatt
     7681 atttagcgta acaactacag gacgcgaaag cattcgccct ttgccttcac tctgcaacaa
     7741 acgcagtact gcatcgacgc gcaaagcctg cgaagctgcg gccggaccca acgctaaacc
     7801 tgcagtaaaa ggtccttgat ctgtgatact gactgttcca tccgttgaaa cacttttgcc
     7861 cctgaattcc agccccaatt gtcgtgaatg agtttcgctg gtttcaaaca agtgggcttc
     7921 aatcctgatc aaaggcaagg gactatccca aacctgcacc agtttttcca tccagtccac
     7981 gtgagttttg ctgccctcca gtatgagcga gttgccaagc aaatccggaa taatttgtgg
     8041 acgcagcgga ggaatttcag cctcgtcttc aagcctttgc tgtagtgctt ttctcaagct
     8101 ctcttctaca ccgggaattg tcaggggctg cccgttgctc tgtacagttt gcggccccac
     8161 acttaagtac ttcaacttca gtcgtacaat tctggtttcc ggaattaatt tcggttctat
     8221 accttcagat tcaaccgcag gtgatgcagg aaacggttcc acatcagctg atgatacttt
     8281 ctggagtccg ctttcccatt gttcaatcag ttgtactgtt aaaacaacgg attcttcgga
     8341 accccttaag atcaacgatt tttcggttgg ataagaatcg tgtacgaggc ggatttcttc
     8401 cagctctttt ctgatctcag tcaaaaaaga ctggacttgt ttttgacttg ccgagctgag
     8461 gacaactgac tttgtcacca acggcggatc ctgggtccat tctaaaacat gcagcctcgg
     8521 ataataaacg acaaccgcat tctgatgact tgccaagtca aatattgcat cctcaattga
     8581 ggaataattt aagttgcgct gtacttttcg tccctgtatt ttatctgaat caaaatgtaa
     8641 agcaatttgc ccaacttgag ataactcacg aagaattttt tccaaaaaaa ccggtgagtt
     8701 gaggattttc ctgaatggtc ggtgccgcca tgcatctttc ctggaaatga tcaggtgccg
     8761 atcctgctga gagactaatg tcaaatcgtg ccgtaaaagc agcagactta gaacagtttc
     8821 tggattatgc ccccgtactt ccttaacttc cggaatacct gtaactgaag aatcaacctt
     8881 tattttttcc ggatacaggg caaactcttg aaaaagcttt tccaacatta caggcaaagt
     8941 tgctgagact actgtggaaa gtacgactcc ggacttgtcg gttcgtttcc agacaattcc
     9001 ggactctgca cataattctg aaaagcctgt aataaagaaa atcattatgc ataataacca
     9061 ctttttcatt gcctcctacc tcaatatttg aagccaattt catcagcaga tgagttagac
     9121 caaaactttt gacctgctcc tgaatacaac tatttttgca attacacaag tgaagtaaaa
     9181 aatttctgcc taaagcaaaa gtaggaaatg tgttgtcaat tccagaataa tgaattaagt
     9241 tttcactagg actgtatagt ttgtttgcac aaatttcaaa aaatttatta acttgtattt
     9301 aaggagattt atgggaaagc tcattcgtcg tccagatatt tggataagtc tcgcatt
//
LOCUS       DTVD01000093            2347 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_19507_length_2347_cov_279.665794, whole genome
            shotgun sequence.
ACCESSION   DTVD01000093 DTVD01000000
VERSION     DTVD01000093.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19507_length_2347_cov_279.665794"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(214..435)
                     /locus_tag="EYO68_03345"
     CDS             complement(214..435)
                     /locus_tag="EYO68_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIC06869.1"
                     /translation="MAIVKSSLSGLEGVTASKISLTEAFVWYNPQMTNPEQIVAEITK
                     SGYGAEVLEVKSVESNSIPKNCLILDLFC"
     gene            complement(574..903)
                     /locus_tag="EYO68_03350"
     CDS             complement(574..903)
                     /locus_tag="EYO68_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06870.1"
                     /translation="MQIEDLKPGLIGVGTSFGASICCVLPMTIVLLGLGSGGFMMVTM
                     QYRIILYPLGLLGLVTAYWLYFRRRKVCDAQACRMQGKRWNIAGLVFSTVLMSLVTYV
                     DFFLVSL"
     gene            complement(914..1255)
                     /locus_tag="EYO68_03355"
     CDS             complement(914..1255)
                     /locus_tag="EYO68_03355"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIC06871.1"
                     /translation="MRCVGPTLSEKELQKFQDQYSFHPDMEEQAEFYNLISNPIRLKI
                     INILREKEAVCVCDLKDIFGVTAAAISQHLAKLKAHRIAKSVKQGQTVFYSLTDHPFL
                     SLIPEKGTLKN"
     gene            complement(1257..1499)
                     /gene="merF"
                     /locus_tag="EYO68_03360"
     CDS             complement(1257..1499)
                     /gene="merF"
                     /locus_tag="EYO68_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system transport protein
                     MerF"
                     /protein_id="HIC06872.1"
                     /translation="MKNKTTFYGGIGTVLTALCCFTPILVIGLTGLGAVAIISYLDYV
                     LFPLLAFFVAITVYGIYRNQQEKQDCERPVSTKAEL"
     gene            complement(1627..2316)
                     /locus_tag="EYO68_03365"
     CDS             complement(1627..2316)
                     /locus_tag="EYO68_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIC06873.1"
                     /translation="MNEINLKPVQHAVFDAYGTLFDVHSAASRHQSRLGEKAQAVSAL
                     WRTKQLEYTWLRSLMKRYVDFWQVTQDALDYALDSNGIDDQSLRRDLLNAYHELACYP
                     EVPETLRNLKELGLGTAILSNGAPKMLEAGVSNSHLEDVLDSVFSVDTIGVFKPAPQV
                     YQMAVDQLGSTPEEILFFSSNAWDVSGAATFGFQAVWVNRFGQAAERLPGTPVLEIKT
                     LDAVLQYLPSD"
BASE COUNT          708 a          537 c          456 g          646 t
ORIGIN      
        1 gttttcaaat ccacttggtc gagctaatga gttttcattc atttttagtt tgtgaaaaag
       61 tttttttctt gcatccctga cttgatccgt gacccagatt ccagatcagg ttaaatacaa
      121 aaattgaaac gattcccctg gattccggaa aaacccttgc agttgacatc tttaatcgaa
      181 caatttctta atgacattag gtcttaaata aactcaacaa aacaagtcta aaatcaaaca
      241 gttctttgga atagagtttg attcaactga cttcacttcc aaaacttcag caccatatcc
      301 tgactttgtg atttccgcta caatctgttc cggattcgtc atttggggat tgtaccagac
      361 gaatgcttcc gttaagctga ttttagatgc ggttacgcct tccagacctg aaagggacga
      421 tttaacaatc gccactcaac cgcctcaagt aagtccggtc aaactcaacg atacttgatt
      481 tagttgtcga gttccctctt gagacaaaac gggagtcgac agcaacagca aaacaactcc
      541 aaaaactaat ccgatttttc gcattatcct ccttcacagt gaaacaagaa aaaaatccac
      601 ataagtcacc agactcatga gaacagttga aaaaacaagt cccgctatat tccagcgttt
      661 accctgcatc cgacaagctt gggcgtcaca gactttcctg cggcggaagt agagccaata
      721 ggccgtcacc agcccgagca gtcccagcgg atacagaata attcggtact gcatcgtaac
      781 catcatgaat cctccactgc ccaatccgag caaaactatc gtcatcggca gcacgcagca
      841 aatcgaggcg ccaaatgatg ttcccactcc gattagcccc ggtttcaaat cctcaatttg
      901 catgatacct ccttcagttt ttaagtgttc ccttttccgg aatcagggat agaaatgggt
      961 ggtcagtcag tgaataaaac actgtctgtc cctgcttgac tgattttgca attcgatgag
     1021 ccttcaattt tgccaaatgc tgagatatag ccgcagcagt cacaccgaaa atatctttca
     1081 aatcgcaaac acagacagcc tccttttcac gtaaaatgtt aatgattttc aggcgaatcg
     1141 gatttgaaat taaattgtag aattcagcct gctcctccat atccggatgg aaagaatatt
     1201 ggtcctgaaa tttttgaagt tctttttccg acaaagtagg tcctacacaa cgcattttat
     1261 agctctgctt tggttgagac gggtcgttcg caatcctgtt tttcctgctg attacggtaa
     1321 attccgtaga cagtgattgc tacaaaaaaa gcaagtaatg gaaacaacac ataatcgaga
     1381 taactgataa tggctacggc cccgagaccc gtcaatccaa tcactaaaat gggcgtaaaa
     1441 caacacaggg cagtcagtac tgttcctatt cctccataga atgtagtttt gtttttcaac
     1501 ttttaccttt aatagtattt aagcatatac taaaatatat aatcttagaa taatagtcaa
     1561 gaagtactta agcatattat tatgtatttg ttcagaaagt ttgaaaaagg agtaatttag
     1621 cagacttcag tctgaaggca aatattgtaa gactgcatcc agcgttttaa tttcaagaac
     1681 cggagttcct ggaagacgct ccgctgcttg tccgaaacga ttgacccaga ctgcctgaaa
     1741 cccaaaagtg gcggctccgg agacatccca ggcgtttgag gaaaagaaca gtatttcttc
     1801 cggagtagat ccaagttgat caacggccat ttgataaact tgcggtgcag gtttaaaaac
     1861 cccaatagtg tcaaccgaaa aaaccgaatc caaaacatct tctaaatgac tgttactgac
     1921 accggcttca agcattttgg gggctccatt tgaaagaatg gctgtcccca atcccagttc
     1981 ctttaaattg cgcagagttt ccggaacttc cggataacag gccaattcgt ggtatgcatt
     2041 caggagatcc cggcgcagac tttgatcgtc aatgccgtta gaatcaagag cataatccag
     2101 agcgtcctga gtaacctgcc agaaatcaac atagcgtttc atcagactgc gcagccaggt
     2161 atactccaat tgtttggtac gccacaatgc cgatacggct tgtgcttttt cacctagtcg
     2221 ggattgatga cgagacgccg cggagtgaac atcaaagagg gtgccgtagg catcaaacac
     2281 tgcatgttga actggtttta aattaatttc attcatgatt attttttaag taaaatgccg
     2341 atactag
//
LOCUS       DTVD01000094           29444 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_199_length_29444_cov_396.876144, whole genome
            shotgun sequence.
ACCESSION   DTVD01000094 DTVD01000000
VERSION     DTVD01000094.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 29444)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29444)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29444
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199_length_29444_cov_396.876144"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(46..969)
                     /locus_tag="EYO68_03370"
     CDS             complement(46..969)
                     /locus_tag="EYO68_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06874.1"
                     /translation="MKIYLKLFGIFGVILLTQISCSSKNYHQPKGQIENLTPSKYQKT
                     PLTRQSVEITKKTYSFKYKFSSPSNEWFEWSWTYKRLETNEMINKFGISKSIFEPFQA
                     TEKNVKSRNRIIKTSLFKKEGKVISPDFNRMIPFYMGFTSPLYALTIRTLGKDSTPRE
                     RVEFLLRFVQDIPYGIPPTRSNSKVISGVLSPPQIFIEKWGDCDSKVLLLSSILAHEP
                     RYKILLLHLDKHLLMAFEGRPHPNDAYIIFQGKKFILADPTGPARLPLGNPGPDFKGQ
                     FKKIEALQVTSADRKIPHYVSRVIEVKKVNL"
     gene            1201..1572
                     /gene="dksA"
                     /locus_tag="EYO68_03375"
     CDS             1201..1572
                     /gene="dksA"
                     /locus_tag="EYO68_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIC06875.1"
                     /translation="MEGTGMLDKKLDKIKIRLEELQTEIIGESDETVHGMQEESTLYP
                     DPNDRASLETEHINNLRIRDRERKLLGKVEEALERIEKKTFGYCEECGQEIDEKRLLI
                     RPVTTFCIICKESLEAVENNN"
     gene            complement(1691..3361)
                     /gene="groL"
                     /locus_tag="EYO68_03380"
     CDS             complement(1691..3361)
                     /gene="groL"
                     /locus_tag="EYO68_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIC06876.1"
                     /translation="MAKQVIFAAENREKLLRGVNALTNAVKVTLGPKGRNVLIDKSFG
                     APLVTKDGVTVAKEIELEDKLENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVVEG
                     HRNLAAGANPMDLKRGIDQAVAAATEQLHKMSKSVSDSKEIAQVGNVSANNDSTIGDI
                     IAESMEKVGKDGVITIEEAKTTETSLEVVEGMQFDRGYLSPYFVTDSERMEVVLEEPY
                     IILVEKKVSNMKDILPAIEQIAKTGNPFLIVAEDIEGEALATLVVNKLRGTLKCAAVK
                     APGFGDRRKAMLEDLAILTGGDVVSEDMGMKLEDLTLAKLGKAKSVVVDKDNTTVIDG
                     GGDKASIKSRINQIRAQIEDTSSDYDREKLQERLAKLAGGVAVINVGAATEIEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRAIETVEKTVKGIKQHDQKVGAEIVLKALET
                     PIRQIVANAGLEGALIVDKVKASSTNEGFDAQEETYVDMISAGIIDPTKVVRTALENA
                     ASVAGLLLTTEAGIHDAPEEKAAGGGMPGGAPDMGGMGGGMGGMGGMM"
     gene            complement(3411..3695)
                     /locus_tag="EYO68_03385"
     CDS             complement(3411..3695)
                     /locus_tag="EYO68_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIC06877.1"
                     /translation="MIRPLQDRVLVQRVEAEEKTASGIIIPDTAKEKPQEGKVVAVGP
                     GKRLDNGSIQEMGVKKGDKILFSKYGGTEVKVDGEDYIIMREDDILGVMA"
     gene            4001..5497
                     /gene="lysS"
                     /locus_tag="EYO68_03390"
     CDS             4001..5497
                     /gene="lysS"
                     /locus_tag="EYO68_03390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIC06878.1"
                     /translation="MEKQADQQQIRLGKLNKIRDLGTEPYPYSFQRSHTIPDIIEQAE
                     ELLKKQEQITIAGRLLAVRGKGKASFGNIQAQHTRIQIYVRQDEIGDSEFEMFKLCDI
                     GDHLGLSGTLMHTKTGELTLRASTVTLLSKSIRPIPMPKVEEKDGERIVHDEVTDKEF
                     RYRQRYVDLILNPQVAEVFQKRTLIYQTIRDYLREEGFLEVETPTLQPVYGGANATPF
                     VTHHKALDQKFYLRISNELYLKRLIAGGFDAVFEFGKDFRNEGIDRTHNPEFSALEFY
                     QAYVDYNEIMRHTENICERCALAVNGRTEFEYEGHTIDVKAPWPRLSMLDAIEKLGGI
                     SFSKMSDAEVQNLLKENGWELDGDYNRGLATQLVFEETCETQLIQPIFITDHPKETSP
                     LCKQNRENPELLERFEAFIYGWEIANAYSELNDPVIQRRLMEEQVERGRGGEKETQPL
                     DEDFLRAMEYGMPPMGGVGFGIDRIVMLLTGQLTIRDVIFFPTMRPES"
     gene            5507..5896
                     /gene="hisI"
                     /locus_tag="EYO68_03395"
     CDS             5507..5896
                     /gene="hisI"
                     /locus_tag="EYO68_03395"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIC06879.1"
                     /translation="MSKNMSEKLKTPEAKLSAADLDFEKLNGLLPVIAQDESSGAVLM
                     LAYANREAVQKTQETGYAHYWSRSRDVLWKKGESSGHLQKIVEVLVDCDNDTLLYKVH
                     QSGPACHTGAPNCFFRELKNETTVKSK"
     gene            5893..7056
                     /locus_tag="EYO68_03400"
     CDS             5893..7056
                     /locus_tag="EYO68_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIC06880.1"
                     /translation="MNWDIQLNEEVDLLRETVRQFAEKRIAPIADEIDRNNEFPQHLW
                     KELGELGLLGITVPEEYGGSGMSYLAHLVAVEEISRASASVGLSYGAHSNLCVNQLKL
                     NGSDAQKKKYLPKLCTGENVGALAMSESGAGSDVVGSMACHAEKHGNYWIANGSKMWI
                     TNGPDANVLIVYMRTAPKDQGSKAMTAFIVEKEMKGFSTAQKLDKLGMRGSNTCELVF
                     EDCEIPNENIIHGVNHGVKVLMSGLNLERIVLSGGPLGIMQAAMDLVLPYVHERWQFD
                     RPIGTFELMQGKLADMYTSLQSARAFCYRIAENCANNQVSRRDTASCLLFASENAVKV
                     SLEAIQALGGNGYINDYPAGRLLRDAKLYDIGAGTNEIRRMLIGRELFDETAT"
     gene            complement(7073..7408)
                     /locus_tag="EYO68_03405"
     CDS             complement(7073..7408)
                     /locus_tag="EYO68_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06881.1"
                     /translation="MKNLFLSATVFLLVFNWTGMHQIFANDSKEIQNVLELKRQGWEV
                     IEKQSRVESRPGLKPYQNLKREVQVVKYRLSKNEQFYFCLVEYDSQLDTIRESCADSE
                     KEAREHLGR"
     gene            complement(7410..8126)
                     /locus_tag="EYO68_03410"
     CDS             complement(7410..8126)
                     /locus_tag="EYO68_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_773797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC06882.1"
                     /translation="MKKAVIIGVGTEQGLGAQLAKRFASEGLHVFVASRTQSRLDALT
                     VEIEQAGGKATAVCADATNEEQVIKLFEKAGSGLDLAIYNTGNNYPGQIIDMDAEYFK
                     KSWKSCCFGGFLFGREAVRHMVKSGRGTLLFTGASASLRGRANFGAFNSAKAGLRTLA
                     QAMAKEYGPKGIHVGHVVIDGAIAGDKIMRHLPELAKKLGEDGMINLEGIVESYVHLY
                     RQSAGAWTFELDLRTSIEKW"
     gene            complement(8259..9473)
                     /locus_tag="EYO68_03415"
     CDS             complement(8259..9473)
                     /locus_tag="EYO68_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIC06883.1"
                     /translation="MSFTSWDVFQKNSRKSSCISTPDMENNKFLRLAKKILWLFFAFF
                     WMIQTASGETILQTSRGGVTFQIKTIAKGLGVPWGMVFISPDQILFTERNGKIGILAP
                     ASGKIIRISGGPKVMRTGQGGLLDVAVPPDYTSGDWIYFTYSKEQKGKGVTALARARR
                     KGNRLVALEDLLVTRSASSTGRHFGSRIAFDGAGHLFFSVGDRGVRPNAQNLSTHAGS
                     ILRLDLNGNVPEDNPFVHQTGALPEIWSYGHRNPQGMFYDKNQQRLWSIEHGPRGGDE
                     INLILPGLNYGWPIISYGKEYWNPFPVGEGTEKEGMEQPVKFYVPSIAPGSLLVYSGK
                     AFPDWKGNLFAGALKLTHLNRVEIDNTGRAITEERLLVGLRERIRALAESPEGWLYLS
                     TDSGKILRIRPQ"
     gene            complement(9436..10017)
                     /locus_tag="EYO68_03420"
     CDS             complement(9436..10017)
                     /locus_tag="EYO68_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase"
                     /protein_id="HIC06884.1"
                     /translation="MSAKTISRFPVPELKELPEDIRERILAVQEKSGFVPNVFLVLAH
                     RPDEFRAFFAYHDALMEKEGGLTKGEREMIVVATSGVNQCIYCVVAHGAILRIREKNP
                     HLADQIATNYRKADITPRQKAMLDFAVKVSIESSDLDESDFEEMRQHGFSDDDIWDTG
                     AISAFFSLSNRMADLTNMRANDEFYLLGRIPKK"
     gene            10216..10872
                     /locus_tag="EYO68_03425"
     CDS             10216..10872
                     /locus_tag="EYO68_03425"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06885.1"
                     /translation="MNLIGDIQGNYRTLLALLKQMPDDDPVSVGDMIDRGPRSMEVLE
                     FFRNHGKALLGNHEHMMLDYFCDSGNYMPGLWLMNSGDATLNSLGLERSGSLPEILQD
                     FLESLPLLLIRDGLLVTHAPVHKRWSLEDIASEDHLELSPLDQSVIWNREDPVPRDGY
                     FQVFGHNGYRYVRAHFFDTRFMIRPESAHAVCIDTVRGQVLSGLHWPSRRIFEQEYVD
                     "
     gene            11016..11723
                     /locus_tag="EYO68_03430"
     CDS             11016..11723
                     /locus_tag="EYO68_03430"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIC06886.1"
                     /translation="MKRLFVFSAFLSVLVLLSNSVQAVDWVSKRTQMIDMHLTPRGIW
                     NPAVLKAMKQVPRHEFVPQHIRKLAYADRPLPIGEGQTISQPYVVAWMSQLLELEKGM
                     KVLEIGTGSGYQAAVLAAMGVKTYSMEIRPNLAREVKIRLKKLGYKVEVRQGDGYYGW
                     NEHAPFDRVIITAAANHVPRPLLDQLKPGGKLILPLGNVRYSQNMTLIEKDSLGKMRT
                     SQHGQVRFVPMVGAAQE"
     gene            complement(11736..14099)
                     /locus_tag="EYO68_03435"
     CDS             complement(11736..14099)
                     /locus_tag="EYO68_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06887.1"
                     /translation="MRVRIPAGMFLVSSAALMYEISLSRLLAIELWNHYAFLIISGAL
                     LGYGAAGAFRLSSSRRIPPLLPVLCFSLLLIPLFLLSSHLPFDPALMALDPRHGVWLL
                     LIFLLLAFPFFLAGLTLNLLLEAYTEHAHFLYASDLIGAACGCAGFFLTAPWLTEIEG
                     LGIPALLAACSSLCLASGKKQNILALATLLTLFCGSFWLGKLELRISDYKNLPHALRY
                     PGSKLLETQRDASVRIDWLETPLARFAPGLSLEFRGSLPDQLGLTLDADGLTGVAPLV
                     PDSLGSYLRHLPEWVLYFKQSPPENVLVLKTLGGQQALAAVEAGTSSVLVQTPYPLLT
                     ERLAEDSRFPQIEFRATEARSLLAEDCPEPCQKPEQNFDRILVAIESSAPVGSTGMDP
                     LKTDQLMSLEGMQSLLNRLAPGGWLAVHRFLLPPPRGEMRLLATVITAMRRQGWKPDQ
                     RLGVFRTLSTLMVLVSREAWTPKESSRFREFCLSRGFAPVYYPDMPETEMNSVIQLQE
                     PVYALGVRELLADPPAFHARTPFDLQPVTDDRPYFELFLDWNRLDDIRKSLGGKWEGL
                     VEAGLLVPLLFAAVSLSALLLIGIPILTHLRRMENTISVLLYFAGIGLAFMLVEIALL
                     EKLTPFLGQPVYSFALVLSGLLTASGLGSFLSSRFSRTGIRFYFLLLLFGLFFCFRNL
                     PDLLRELSGEEWIIRLLWAWLVVSASGLLMGIPFPAGLKHFAVFGKHTEERRIRVAMA
                     WCANACASVAGAAGAVWIAQLAGQSILFLLGALAYGTAWLTLEIRGG"
     gene            complement(14207..15235)
                     /locus_tag="EYO68_03440"
     CDS             complement(14207..15235)
                     /locus_tag="EYO68_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIC06888.1"
                     /translation="MKAMVLNELCGLEENQAPLELTELPDPIPSANEILIRVSACGVC
                     HTELDEIEGRTHPPRLPIVLGHQVVGRIEATGSEANTFRIGDRVGIAWIFSVCGTCKF
                     CLAGNENLCPDFKAAGRDANGGYAQYMKVAEGFAYHIPHFFNDSEAAPLLCAGAIGYR
                     SLRLSGLKDGQNLGLTGFGASGHLVLKMVKHQYPNTKVFVFARSVKEREFSGKLGAVW
                     SGDIEEDSPEKLDCIIDTTPAWLSVIESLKNLEPGGRLVINAIGKEEADKEFLLGLDY
                     PVHLWLEKQIKSVANICRKDVSEFLALAAEMRIKPEIQEFALEDANEALVELKTRRIR
                     GAKVLKID"
     gene            complement(15232..15465)
                     /locus_tag="EYO68_03445"
     CDS             complement(15232..15465)
                     /locus_tag="EYO68_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06889.1"
                     /translation="MPSDTSSIAQLIQEMVDQQRSKVLKVARELVADATPEDIRNPQD
                     FPELSTDALFNYEDGILTGYLSMQTALRSQEKK"
     gene            complement(15485..16246)
                     /locus_tag="EYO68_03450"
     CDS             complement(15485..16246)
                     /locus_tag="EYO68_03450"
                     /inference="COORDINATES: protein motif:HMM:PRK06986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HIC06890.1"
                     /translation="MPTKQNPYTEKTQQSREDQLIKHAPLVKRVVTRMAARFPPGVDQ
                     EELYQVGMIGLLDAVDKFDPTRDVKFKTYAEFRIKGAILDELRKMDWVPRSVRADTNK
                     HSSAWRELSFELGREPSDQEMAGQMKMSMDEYYEFIRKASPTPLISIEEFKGYSNEED
                     SVSLLEILSKPGEKDPFSILSLQQMKTKLAEAIGQLEEREQMVLALYYEEEMNLKEIG
                     EILGVIESRVSQIRSKTILKLRAKLRIMTKDGMEN"
     gene            complement(16263..17555)
                     /gene="flhF"
                     /locus_tag="EYO68_03455"
     CDS             complement(16263..17555)
                     /gene="flhF"
                     /locus_tag="EYO68_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HIC06891.1"
                     /translation="MNLKRYRVNNIKEALKFIKRDLGSDAMIVSTRQVKENTGAFGLF
                     GKTMLEVTASASEKKKDNRSSVPATKALETYGGSSETSETVLADTFLTPRNKSVLSVR
                     DETRKMMLPIQKELQGIRITLGDLSSQSDGLDKHLMGELKHELGEMRHMMHILATHTG
                     SITEPDLPENLLLLYQQMKFSGMEERFSRRLVMEAQLNMTTEDIENFAYVKIFIARML
                     MKIIKITRGMEGLKSPQKIVALLGPTGVGKTTTLAKIASEQMLKYKRKVALISVDTNH
                     SSAAEQIRSFAKKIKAPFSVVKYKAEMNRIIDSYESYDSIIIDTDGCSQRNEKQLYEM
                     REIFDERGRIHNALVLSATSKDSDMSEVTRKFGCMPIDSIIFTKLDESASYGSLFNHA
                     IRFKKPLSYLTVGQKVPEDIEVASKERMVDLLLNISGT"
     gene            complement(17638..19659)
                     /gene="flhA"
                     /locus_tag="EYO68_03460"
     CDS             complement(17638..19659)
                     /gene="flhA"
                     /locus_tag="EYO68_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HIC06892.1"
                     /translation="MSILLIMVVPLPLVILDLLLALSITVGVLILFVALFTKSPLEFS
                     SFPSVLLIATIFRLSLNVASTRLILLEGHQGPGAAGTIINSFGEFVVGGNQIVGIIIF
                     LILVIINFVVITKGTGRIAEVAARFTLDAMPGKQMAIDADLNAGLIDEDEARQRRSTI
                     QRESDYFGALDGASKFVRGDTIAGLIITSINILGGLLVGVTQFDMSAGDAFDSYATLT
                     IGDGLVSQIPALIISIAAGIAVTKASGDNKISVDLHTQIFSNVQALYVVSAVLVSFSL
                     IPGLPIVPFMLLAILTFGLAQVSKRSNVQIAEDEHQEEQVRLQKEISEEPEDIESLLP
                     IDILGLELGYGMIPLVDEEQDGELLKRIKAIRRQIALDFGYIVPPLHIKDNLELSPGE
                     YSITIKGIEIARNELMMGHHLAMKTGDVDEEIPGVDTVEPAFGLPAIWIAEEDEERAQ
                     FAGYTVVDLPTVLATHLTEVLKNHAFEFIGRQETQKLIDSHAESEPKVVEELIPSLLS
                     LGVVQKVLQNLLKELVSIRDLHTVLETLADVSLITKDPDLLTEHVRQSLSRQITRQYQ
                     TPDGLLPLITMNQDLENQIATAIQDSGQGAYLGLNPNMAQAIINGIDGMIEQFTINNY
                     QPLLLCSPLIRPHVKKLVERFIPNLVVLSHNEVAQDVRIEALGVVELSG"
     gene            complement(19887..20951)
                     /gene="flhB"
                     /locus_tag="EYO68_03465"
     CDS             complement(19887..20951)
                     /gene="flhB"
                     /locus_tag="EYO68_03465"
                     /inference="COORDINATES: protein motif:HMM:PRK05702.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HIC06893.1"
                     /translation="MAEQEGQEKTEVPTEKKRRESREEGQVAFSKELSSAALLAGIVL
                     TLVATSPIILDAMRQLMSQIFRDLAQSEELSIDSIFTLSGEILSIILPAFAPFAAVII
                     FVGIFASVLQVGVLITFKAIAPKFNKISPLTGLKRLFSSQSLADFLKSMAKLIIVGFV
                     GYLTYIEKITELNGLSVSTPESILKYNFTVVAEVAGKIVLALVAIAIFDYFYQRWHHE
                     QQLMMTKQEVKDETKQTEGDPQLKARIRQIQREMSNARMMQEVPKADAVIVNPTHFSV
                     AILYDRDVMTAPEVIAKGADHLALRMRTVARENNVPILERPELARDLYANVEIGDDIP
                     ERFYKAIAEILAFVYRLRKR"
     gene            complement(21058..21840)
                     /gene="fliR"
                     /locus_tag="EYO68_03470"
     CDS             complement(21058..21840)
                     /gene="fliR"
                     /locus_tag="EYO68_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HIC06894.1"
                     /translation="MANLLSYDASQLLAFILVLVRVSGIISTAPVFGSSNIPPQIKVV
                     LSLMLALILYPFIPLITVYPDRPDHYIMLIASEMLIGLVLGIIARFLFGAVEFAGTVI
                     GFQMGLGMANVFDPQSQEQISLVGRFENTTATLIFLAMDGHLIVLQALVRSYSVLPPG
                     GASISRPLVENLIELSASVFVIGLQIGAPLIVALFLANAVVGLLARSVPQIQVFVVGF
                     PLTLMLGFLFLFFGMPFFAQAVHQMFEKLDTQFFEAIKLLGG"
     gene            complement(22004..22393)
                     /locus_tag="EYO68_03475"
     CDS             complement(22004..22393)
                     /locus_tag="EYO68_03475"
                     /inference="COORDINATES: protein motif:HMM:PF01258.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIC06895.1"
                     /translation="MEDIKRLLLEEKAALLSGIQEKTENKTEINKDIGDAIDSSVEEQ
                     KRELDLLLQDREQARLTGIEIALKRIESDDFGYCDECGEAISKKRLLAVPLARMCINC
                     QSNEERSRLAQNTFSRADYSSQILVDD"
     gene            23001..23819
                     /locus_tag="EYO68_03480"
     CDS             23001..23819
                     /locus_tag="EYO68_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06896.1"
                     /translation="MQQINNKCSKSIFICCLYMTGFLLTETAIFGQESYLSELLPGTE
                     SSFQHLNLESSKITGKSHFRYETLIKEGTRFVVEKNENTKTDGEVFTRKTLWFDADSG
                     IPKWYEEEDLRKEFRITNSYTGQIMHTRLVEDGQILEFKTNLSAENAVPFEVVIFFLR
                     KYLKQILQTKNFSFTLFIPLLAIELEKKGLPRSMSMISMWAEPQEKIRMDTPLGVMNA
                     HTILVSPQSGLLRALLPREKTHFEFTFAAASPYHLLQFKAGKTRHVLTSLILPE"
     gene            complement(23895..24362)
                     /locus_tag="EYO68_03485"
     CDS             complement(23895..24362)
                     /locus_tag="EYO68_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIC06897.1"
                     /translation="MEFQLTSGLQPELLEDDQKQWLAYAICGAITSDGSVAPEELEYL
                     ERALAFLPSETEVNNMMRAVKDQKLPELDRFPGATREMEVKIFIDLTSVISVDNVLGA
                     NEIDYLLHIGRKLGFGREFVRVVIRWASEGIVWRRKMQHLIDAGSSLEAEYTD"
     gene            24722..26062
                     /locus_tag="EYO68_03490"
     CDS             24722..26062
                     /locus_tag="EYO68_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIC06898.1"
                     /translation="MNQVSVYVLDISVLLCTPGALFEFPDKEIVIPVTILEELDSLKL
                     DLGEKGRSAQIVSQMLDECRQYGSLVEGISLPNGGKLRIELTEPESGLLPYSLNLKRI
                     SNRVLAVAWMLSQKNKDLILVSQDENLRTKANTLNVPTLSYNGQRPNDSNLYAGIRQS
                     EVSKQKLRSLGKQSYISPEEVFSDQNEICEFYPNEGLLLSCTDVPDEQILATYQQGKK
                     KFELVPKEQGVWGIRPLNPEQRLALALLMNPKISVVTLSGISGTGKTLLALAVGLQQL
                     MVDNIYSRMLVSRPIFPMGRDLGYLPGDTQEKLAPWMQPIFDNLELLINNPASKNGSK
                     HDRYNELMDRGMLVVEPLTYIRGRTIPNQYMIVDEAQNLTPHEMKTILTRVGEGTKIV
                     LTGDPNQIDNSEVNLSSNGLSTLVERFKESPLAGHVRFTSVERSPLAELAATVL"
     gene            26222..27475
                     /gene="coaBC"
                     /locus_tag="EYO68_03495"
     CDS             26222..27475
                     /gene="coaBC"
                     /locus_tag="EYO68_03495"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIC06899.1"
                     /translation="MSLNWNFEPPPPSEFADREVRLTGSHLSGKQIALLITGSIAAYR
                     MPDLIRDFRREGADVVVYATTEGLRYVAREALEWCSQNPVIEGFTSDAEHLSDSTPFD
                     AFVVAPASYNTLNKASLGIADSVVTAAIAAALGRMERQGIPVLFAPAMHGAMHNSILT
                     RSMRTLQEMGVTLIPPHQSHGKNNLAPLELIVATTIRALSESPLRGKSLLITGGPTPV
                     PVDNIRRITTRFTGALSIQLAREAWMRGAAVELILGKGSRSAPEYIKAKTAETFEDYR
                     RTLKESLQEGNYDWGIFTAAVADFQPESVFEGKLPSNQKQLSLKLVPTVKVIEEVRQH
                     FPELKMVTFKYEENISHEELMEIAKNRLSEKGGSQLVVANRVEEFKQDGTQVAWLLES
                     KQEPQKHIGKKDIANAILDRIELTA"
     gene            27577..28179
                     /locus_tag="EYO68_03500"
     CDS             27577..28179
                     /locus_tag="EYO68_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIC06900.1"
                     /translation="MVDRWANTVVHPTMDDIANFVPRQISANSITFIGFGIGLLAVPL
                     LWLKLYPAALVLILVNRFFDGLDGAVARRNGVTNLGGYLDITCDFIFYSAVIVGFALA
                     SPENNSLTATFLIFSFIGTGTSFLAFAVIAEKHGISSAAHGQKAFFYLGGLTEGTETI
                     IFYIIICLFPEYFPILAVVFGGLCWITVVGRFGSAYSLLR"
     gene            complement(28254..29423)
                     /gene="doeA"
                     /locus_tag="EYO68_03505"
     CDS             complement(28254..29423)
                     /gene="doeA"
                     /locus_tag="EYO68_03505"
                     /EC_number="3.5.4.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydrolase DoeA"
                     /protein_id="HIC06901.1"
                     /translation="MPAFEKEEYEIRISKVRKSMDEKNIEVLIVTDPSNMAWLTGYDG
                     WSFYVHQCVVLSLEGEPFWYGRGMDANGAKLTVFMQHENIIGYPDDYVQNPEKHPMDY
                     LSEILTEKNWAQKTIGVEKDNYYFSASCLESLQRNLVQANFVDSTGLVNWQRTVKSPR
                     ELEFMRKAARIVEKMHARIFEVMEPGMRKNDLVAEIYHSGITGAEDAGGDYAAIVPMT
                     PTGSDASAPHLTWDDQPIPNNSGTFFEIAGCYRRYHCPLSRTIYLGQPPQKYLDVEKA
                     VLEGIEAGLEAAKPGNFAEDIEAAWRKTIAKYGYEKESRCGYAIGLSYPPDWGERTVS
                     FRKGDKTVLEPNMTFHFMPALWFDDWGLETTESIVITDSGVETLANVPRKLFVKP"
BASE COUNT         8178 a         6675 c         6323 g         8268 t
ORIGIN      
        1 tcgggatgac acgacaaatc tcgtgactat cgagttactg atttgttaaa gatttacttt
       61 tttaacttca atgacccttg acacatagtg cggaattttt cgatctgcag aagtgacttg
      121 aagagcttca attttcttaa attgtccctt aaaatcgggg ccgggattcc ccagaggcag
      181 acgcgctggt ccggtgggat ctgccagaat aaattttttc ccttgaaaga ttatgtaagc
      241 atcatttgga tgagggcgtc cttcaaaggc cattaacaga tgtttgtcta aatgtaacaa
      301 caaaatttta tatcgaggtt catgagccag gatggacgat aataataaaa cctttgaatc
      361 acagtctccc catttttcaa tgaaaatttg cggtggcgac aaaacacctg atatcacttt
      421 tgaatttgaa cgagttggag gaataccgta gggtatatcc tgaacgaaac gtaagagaaa
      481 ttctacacgt tcccttgggg ttgaatcttt tcctaatgtt ctgatcgtta aagcataaag
      541 aggtgacgta aaccccatat aaaaagggat catacgatta aagtcaggag atattacttt
      601 accttccttc ttaaataatg aggtctttat tattcgattt cttgatttaa catttttttc
      661 tgtagcctga aaaggttcaa aaatggactt ggagattccg aatttgttta tcatttcatt
      721 ggtctcaagc cgtttataag tccaactcca ttcaaaccac tcattggaag gagatgaaaa
      781 tttatatttg aaactataag tttttttggt gatttcaaca ctttggcggg ttaaaggggt
      841 tttttgatat tttgaaggag tgagattttc tatctgtccc tttggttgat gatagttttt
      901 tgaagaacat gaaatttgag ttaataatat aactccaaaa atgccgaata atttcaggta
      961 aattttcatc taaatccaac gaggtgtgct cacaagtata gccggtgcgg gaatctcctc
     1021 aaccggcgac ttgtagaaaa ttctaacagt gttagcttga cgttaccagt ttttcaaatc
     1081 gcattttgac ctctgtagct tttaagaagc ttaattgaaa tttttcaata aaaaactttc
     1141 gagattttta aaaaactgta aaatgggtgg taacaaatta atgaaaaaaa cgcattctat
     1201 atggagggaa caggaatgct ggacaaaaag ctggacaaaa tcaaaatacg tttggaagaa
     1261 ttgcaaacgg aaattattgg tgaatctgat gaaaccgtac acggcatgca ggaagaaagt
     1321 acgctctatc ctgatcccaa tgatcgggcc tcactggaaa ctgaacacat caataattta
     1381 cgaattcggg accgggagcg caaactactt ggtaaggttg aagaagccct ggaacggata
     1441 gaaaagaaaa catttggata ctgtgaagaa tgcggtcagg aaattgatga aaagcgattg
     1501 ctcattcgac ctgtaaccac tttttgtata atctgcaaag aatccctgga agcagtagaa
     1561 aataataact gattccgcct tagaatcttc aaagttaatt cctcttttaa aataatccaa
     1621 aaacaaaaaa ggcgggctga ctctcagagc cgacccgcct ttattaatca gaatggattc
     1681 tgaaccggaa ttacatcatt ccgcccatac ctcccattcc gccgcccatt ccgcccatgt
     1741 caggagcacc acccggcatt ccgccaccag ctgctttctc ttcaggggca tcatgaatac
     1801 cagcttcagt agtcaaaagc aatccggcta cagatgctgc attctccagg gcagttcgaa
     1861 caactttagt cggatcaatg attccggctg aaatcatgtc aacataagtt tcttcttgtg
     1921 catcaaagcc ttcgtttgtg ctgctggctt ttactttatc gacaatcaac gcaccttcga
     1981 gtcctgcatt agctacaatc tgacgtatag gagtttcaag tgccttaagt acaatttcag
     2041 caccaacttt ctggtcatgc tgttttatgc cttttacagt cttttcgaca gtttcaatag
     2101 cacgcagcaa agcaactccg cctccaggaa caattccttc ttcaacagcg gcacgggttg
     2161 cgtgcagtgc atcctctact cgagccttct tctctttcat ctcgatttca gttgcagctc
     2221 caacgttaat cacagctaca cctccagcca atttagcaag acgctcctgc aacttttcac
     2281 gatcataatc agaagatgta tcttcgatct gagcacgaat ttgattgatg cgggacttga
     2341 tgctggcttt atcaccacca ccgtcaataa cagtagtgtt gtctttgtca acgaccacag
     2401 acttggcttt cccaagttta gcaagtgtca ggtcttcgag tttcattccc atatcttcgg
     2461 aaactacgtc tcctcctgta aggatcgcga gatcttccaa catggccttg cggcggtcac
     2521 cgaaacctgg agctttaaca gctgcacact tcagggtgcc acgcagtttg ttgacaacga
     2581 gggttgctaa tgcttcacct tcaatgtctt ctgcaacaat caggaaagga ttacctgtct
     2641 tggcgatttg ctcaattgca ggaagaatat ccttcatgtt actgaccttc ttttccacca
     2701 gaataatata aggttcctcc aggaccactt ccatccgttc tgaatctgta acaaagtacg
     2761 gagaaagata gccgcggtca aactgcattc cttctacaac ttccaaactg gtttcagtag
     2821 tctttgcttc ttcaatggtt atcacaccgt ccttacctac tttttccatg gattcagcaa
     2881 taatgtcgcc aattgtggaa tcattgtttg ctgatacatt acccacttga gcgatttcct
     2941 tggaatcgga aaccgacttg gacattttgt gcaactgctc agttgcagca gcaactgctt
     3001 gatcaattcc acgcttgaga tccattggat tcgctcctgc tgccaggtta cgatgacctt
     3061 cgaccacaat cgcttgtgct aacacagtag cagtggtggt cccgtcaccg gcaacatcag
     3121 aagtttttga agcaacctct ttgaccatct gcgcacccat attttccagc ttgtcttcca
     3181 gctcaatttc tttagcaacg gttacaccgt ctttggtaac aagtggagcc ccaaaagatt
     3241 tatctattaa aacattccgg cctttaggac ccaatgttac tttgactgca tttgtcaagg
     3301 catttacgcc tcgaagcagt ttttcgcggt tttccgccgc aaaaataact tgttttgcca
     3361 taccttttct ctaattaaat taatgttata ggtttttcag taatctgtaa ttaagccata
     3421 acgccaagga tatcatcctc acgcatgatt atataatctt cgccatcgac ttttacctcg
     3481 gtgccgccat acttactgaa taaaatttta tcaccttttt ttactcccat ctcttgaatg
     3541 gaaccattgt caaggcgttt gccaggccca actgcgacca ctttaccttc ttgaggcttt
     3601 tctttagctg tatccggaat gataataccg gaagctgtct tttcttcggc ttcgacgcgc
     3661 tgtacaagca cgcggtcctg taagggacga atcatagttc tccttttagg attataatta
     3721 attaaataaa ttgagcttca acaacaataa gcgtatgcta agccgtttaa gtgaatctta
     3781 aatttcgaaa attattcaaa tcttgattca ggataataat tctagcgtaa taattaatta
     3841 aaggcaagta aaaaatagca ctcaacaatg agtagtgcta attttttaat ttttcgatgt
     3901 taaaagttgg agcggtcttt tcctttaacc atataacatc attctctcaa catattaata
     3961 ttattaagta aaacagctta taaacagaaa aattttatag atggagaaac aagcagatca
     4021 acaacaaatc cggcttggta agctgaataa aattcgtgat ttgggtacag aaccttaccc
     4081 atattcattc caaagaagcc atacaattcc ggatattatc gaacaggcag aagaactgct
     4141 gaaaaagcag gagcaaataa ccattgcagg ccgcctgctt gcggtacgcg gcaagggcaa
     4201 ggccagtttt ggcaacattc aagcccagca cacgcgtata caaatatatg tacgtcagga
     4261 tgaaatcgga gattcagaat tcgaaatgtt taagctgtgt gacatcggtg atcatcttgg
     4321 tctttcagga acactgatgc acactaaaac cggagaactg actttgaggg cgagcactgt
     4381 cactttactc tcaaaatcaa ttcggccaat tccgatgcca aaggtagaag aaaaggatgg
     4441 agagcgcatt gttcacgatg aagttacaga caaagaattc cgctatcgtc agcgatatgt
     4501 tgacttaata ctgaatccac aggtagcaga agttttccaa aagcgtaccc tgatatacca
     4561 gactattcgt gactatttac gggaagaagg ttttttggaa gtagaaacac cgacactcca
     4621 accggtatac ggcggtgcca atgctactcc ttttgttaca catcacaagg cactggatca
     4681 aaaattttat ttgcggattt caaatgagtt gtacttgaaa cgtctcattg caggaggctt
     4741 tgatgcagtt tttgaatttg gcaaagattt ccggaacgag ggtattgaca ggacccacaa
     4801 tccggaattt tctgcactgg aattttatca ggcctatgtt gattacaacg aaataatgcg
     4861 tcacacagaa aatatttgtg aacgctgcgc gcttgcggtc aacggcagaa ctgaatttga
     4921 atatgaaggg catactatag atgtgaaagc gccgtggccg cgtttaagca tgcttgatgc
     4981 aattgaaaaa ttgggtggga tttcgttctc aaaaatgagt gacgcagaag tccaaaattt
     5041 acttaaagaa aacggatggg agcttgatgg tgattataat cggggattgg caactcagct
     5101 tgtgtttgaa gaaacttgtg aaactcagct tattcagccg atttttatca ccgatcatcc
     5161 aaaagaaacc agcccgcttt gtaaacaaaa tcgtgaaaat ccggagctgc ttgagcggtt
     5221 tgaagccttc atctacggct gggaaattgc taatgcgtat tcggaactga atgaccccgt
     5281 gattcagcgc cgtttgatgg aggaacaagt cgagcgggga cgcggtggtg aaaaagaaac
     5341 tcaacccctg gacgaagatt tcttacgggc aatggaatat ggaatgccgc cgatgggtgg
     5401 agttggtttt ggaattgacc ggatagtaat gctgcttacc ggacaattga caattcgcga
     5461 tgttatcttc tttccaacca tgcgtcctga atcgtgagga gacataatgt caaaaaatat
     5521 gtccgaaaag ctaaaaactc ctgaagccaa actttctgct gcagatttag attttgaaaa
     5581 attgaacggg ctgcttcctg tgattgcaca agatgaaagt tccggagcag ttttgatgct
     5641 ggcttacgcc aaccgggaag cagtgcaaaa aacgcaggaa actggttatg cacactactg
     5701 gtcacgatca cgtgatgtgc tttggaaaaa aggtgagagt tctggtcatt tgcagaaaat
     5761 tgtggaagtg ctggtggatt gtgataatga tactctgctt tacaaagtcc atcaatccgg
     5821 acctgcgtgt catacaggtg cgccgaactg ctttttccgg gaattgaaaa acgaaacgac
     5881 agttaaatca aaatgaactg ggatattcaa cttaatgaag aagtagatct gctgcgggaa
     5941 acagtccggc aatttgctga aaaacgcatt gcaccgattg ctgatgaaat agaccgcaac
     6001 aatgaattcc ctcagcattt gtggaaggag cttggcgaac tgggactcct gggtattact
     6061 gttccggaag agtatggagg ctccggaatg tcttatttag ctcatttggt tgcagtagag
     6121 gaaatctcaa gagcatctgc ttcggtcggc ttgtcctatg gagcacactc gaatttgtgt
     6181 gtaaatcaac ttaaactgaa cggcagtgac gcgcaaaaga aaaaatatct accaaaactt
     6241 tgcaccggtg aaaatgtcgg tgcgctggcc atgtctgaat ccggagcagg ttcggatgta
     6301 gtaggttcaa tggcttgtca tgcagaaaag catggtaact actggatcgc caatggttca
     6361 aaaatgtgga tcaccaatgg tcctgacgcc aatgtgctga ttgtttacat gcgcactgct
     6421 cctaaagatc agggatcaaa agcaatgacc gctttcatcg ttgaaaaaga aatgaaaggt
     6481 ttttcaacgg ctcagaaatt ggacaaactc ggtatgcgcg gttccaatac atgtgaactt
     6541 gtttttgaag attgtgaaat tccaaacgaa aacataatcc atggggtcaa tcatggagta
     6601 aaggtactga tgagtggact aaatttagaa aggattgtgc tttccggagg accacttgga
     6661 atcatgcagg cagcaatgga tttggtgttg ccttacgtcc atgaacgctg gcagtttgat
     6721 cggccgattg gcacttttga acttatgcag ggaaagcttg cggatatgta tacttcactg
     6781 caatctgcca gagctttttg ttatcgcatt gcagaaaatt gcgcaaataa ccaggtttca
     6841 cgcagagata ccgcttcctg cttattattc gcatcagaaa atgcagtaaa agtttcactg
     6901 gaagcaattc aggctttagg gggaaacggc tacataaatg attatccggc tggacggctg
     6961 ctgcgtgatg ctaaacttta tgatattgga gcaggtacca acgaaattcg gcggatgctg
     7021 attgggcggg aactttttga tgagactgct acctgatgcc gtcctgacca ctctatcggc
     7081 caaggtgctc tctcgcctcc ttttcactgt cagcacagga ttctctgatg gtatccaact
     7141 ggctgtcgta ttcaacaaga cagaaataaa actgctcgtt tttgctcagc cgatatttaa
     7201 ccacctgaac ttctcgtttc aggttctggt aaggtttcag tcccggacgg gattccacac
     7261 ggctttgttt ttcgatgact tcccatccct gccgtttaag ttccaataca ttttgaattt
     7321 ctttggaatc gtttgcaaaa atttgatgca tcccagtcca gttaaaaacc agcaagaaaa
     7381 cggtggcact caggaaaaga tttttcattt taccactttt ctatggaggt acgaagatcg
     7441 agttcaaatg tccatgctcc cgctgattga cgataaagat gaacatagct ttcaacaata
     7501 ccttcgagat ttatcatacc gtcttctcct agttttttag caagttcagg cagatgacgc
     7561 ataatcttgt ctccggctat ggcaccgtca attacaacat gccctacatg aattcccttc
     7621 ggaccatatt ccttggccat cgcctgagca agtgtgcgca atcctgcctt tgctgaatta
     7681 aaggcaccga aattggcgcg tccacgcagt gaagcgcttg caccagtgaa tagcagagtc
     7741 cccctaccgg acttgaccat gtgacggaca gcctcccggc caaaaagaaa acctccaaag
     7801 cagcatgatt tccagctctt cttgaaatat tctgcatcca tatcgatgat ttgacctgga
     7861 taattgttgc cggtattata gattgccaag tccagcccgc tgcctgcttt ttcaaaaagc
     7921 ttaatcactt gctcttcgtt ggttgcatcc gcgcaaacag cagttgcctt acctcctgct
     7981 tgttcaattt caactgtcag cgcatcaagt ctggactgag ttctgctggc gacaaaaaca
     8041 tgaagtcctt ccgaggcaaa acgtttagcc agttgagctc caagtccctg ttctgttcca
     8101 actccgatga tgactgcttt tttcacataa cctcttttta tctactcaga ttctactgac
     8161 attaattaaa aactttttct gtttaaaccg taaaataata atgccgcatt tgtttgtctt
     8221 ttcaggtttt taaacaaaca ttctacaatc attgattttt attgcggcct gattctcagg
     8281 atttttccgc tgtcagttga cagataaagc caaccttccg gactttcggc taaagcacgt
     8341 atgcgttctc tgagccccac aagaagtcgt tcttcggtaa ttgcccttcc ggtgttatca
     8401 atttccacac gattcagatg tgtcaacttt aaggcgcctg caaacaaatt tcccttccag
     8461 tccggaaagg ctttcccgga atagacaagc aaacttccgg gggcaatgga tggaacatag
     8521 aatttgaccg gctgttccat gccttctttt tcagttcctt ctcctactgg aaaagggttc
     8581 cagtattcct ttccatagga aattatcggc cagccgtagt ttaatcccgg caggattaga
     8641 ttgatttcat ctcctccgcg gggaccgtgc tcaattgacc agagacgctg ctgatttttg
     8701 tcatagaaca ttccctgtgg attgcggtgt ccataactcc agatttccgg caatgcgccc
     8761 gtttgatgca caaatggatt gtcttcaggc acattgccgt tcaagtccaa acgcagaatt
     8821 gaaccggcat gtgtggacaa attctgagca ttgggacgta cacctcggtc accaactgaa
     8881 aaaaatagat gccctgctcc atcaaatgct atgcggctcc cgaaatgtct tcctgtgctg
     8941 cttgcagaac gagtaacgag taggtcctct aatgctacaa gacggttgcc ttttcttctg
     9001 gctcgtgcca gtgcggtaac tcccttgcct ttctgttctt tgctgtaggt aaaataaatc
     9061 cagtctcccg acgtataatc aggaggaacg gctacatcca gcaggccacc ctgtcctgta
     9121 cgcattactt tcggtccgcc gctaatacgg attatttttc cgcttgcagg agcaagaatt
     9181 ccaatcttcc cgttacgttc ggtaaataag atttgatccg gactgataaa gaccatgccc
     9241 cacggaactc cgagtccttt tgcgattgtc tttatctgga atgttacgcc acctctgctt
     9301 gtctgcaaaa ttgtttcacc ggatgccgtt tgaatcatcc agaagaatgc aaaaaacagc
     9361 cacagaatct ttttcgccaa acgaagaaat ttgttatttt ccatatcagg cgtacttatg
     9421 caggagcttt tccggctatt tttttggaat acgtcccagg aggtaaaact catcatttgc
     9481 ccgcatgtta gttaaatctg ccattcgatt ggataaggaa aaaaaagctg aaattgcacc
     9541 cgtgtcccaa atgtcatcat cagaaaaacc gtgttgacgc atttcctcaa aatcagattc
     9601 gtccagatca cttgactcta ttgaaacttt gactgcaaaa tcaagcattg ctttttgcct
     9661 tggagtgatg tcagccttgc ggtaattagt tgcaatctgg tctgctagat gcgggttttt
     9721 ctctcgaatt cttaaaattg caccgtgtgc tacgacacag taaatacatt gattgacacc
     9781 agaagttgca acaacaatca tttcgcgctc gcccttcgtc agtcctccct ctttttccat
     9841 caaggcatca tgatatgcaa aaaaagctct gaactcatcc ggacgatgag caagtaccag
     9901 aaaaacattt ggaacaaatc cggacttttc ctgtactgcc aggatccttt cccggatgtc
     9961 ttccggcaat tctttaagtt cgggaaccgg gaagcggctg attgttttag ctgacattat
    10021 ggtgcctttc tttttatagt ttgttgttga tcgttttttt tggattacat cagagatttt
    10081 aaatcgtttc cttaaaattc ttcctcgcat ttatctggtt gaagttatat attattggtt
    10141 catcttgtca agaagtaaat tgattttcga tgcagccggt gatttcgttt aactgcatcc
    10201 tgaccaaaat aataaatgaa cctgatcgga gacatccagg ggaattaccg gactctgctg
    10261 gcactgctga agcagatgcc ggacgatgat cctgtttcgg tgggagacat gattgaccgc
    10321 gggccgcgct ccatggaagt gcttgagttt ttccggaatc acgggaaggc gttgctggga
    10381 aatcacgagc atatgatgct cgattatttc tgtgacagcg gaaattacat gcctggtctc
    10441 tggctgatga acagcggaga tgcaaccctg aattctttgg gactggagcg ctcgggttcc
    10501 ctgcctgaaa tactgcagga tttcctcgaa tcactcccct tgttgctgat ccgagacgga
    10561 ctgcttgtga ctcatgcacc tgttcacaaa cgctggagcc tggaggatat tgcttctgaa
    10621 gaccaccttg aactaagtcc tcttgatcag agtgtgatat ggaacaggga agaccctgtt
    10681 ccccgtgacg gatatttcca ggtattcggt cacaacggct accgttacgt ccgggctcat
    10741 tttttcgata caaggttcat gatccggcct gaatcagcac atgcggtttg catagacact
    10801 gttcggggtc aggttttgag cggactgcat tggccctccc gccggatatt tgaacaggaa
    10861 tatgttgatt aaaggcatga agaaaaacac tttcagcaag aattgaacaa gcaggatgca
    10921 gacgagttat ccattttggc agaaaacctg aaagcataga tgtattagat cctgcaacag
    10981 gaaaagtgat gttaaccttc ataaaaaatt tcaggatgaa gagattattt gttttctctg
    11041 catttctttc agtactcgtt cttctctcga attccgtaca ggcagttgat tgggtgtcca
    11101 aacgaacgca gatgatcgac atgcacctca caccgcgggg aatctggaac cctgccgtgc
    11161 tcaaggcgat gaaacaggtg ccgcgccacg aattcgtacc gcagcacatt cgcaaactgg
    11221 cttatgccga ccgtcccctg ccgataggcg agggacaaac catcagccag ccttacgtgg
    11281 tggcctggat gtctcaactg ctggagctcg aaaaaggcat gaaggtgctg gaaatcggaa
    11341 cgggttcggg ttaccaggca gcagtgctgg cggccatggg agtgaaaacc tattccatgg
    11401 aaatcagacc gaaccttgcc cgtgaggtca aaattcggct gaaaaaactt ggttacaagg
    11461 tggaggtgcg tcaaggtgac ggttattacg gctggaatga gcatgcaccc tttgacagag
    11521 tcatcatcac cgcggctgcc aatcatgtac cgcgtccatt gctcgaccag ctaaaacccg
    11581 gtggtaagct gatcctgccc ctcggcaatg tgcgttactc tcaaaacatg actctgatag
    11641 agaaagactc actgggcaaa atgcgtactt cgcagcatgg acaagtccgt tttgtgccga
    11701 tggtcggagc ggcacaggaa tagccgttcc ctccttcacc ctccccgaat ttccagggtc
    11761 aaccacgcgg ttccataagc caacgcaccc agcagaaaca gaattgactg tccggccaac
    11821 tgtgcgatcc atactgcgcc ggccgctccc gccactgaag cgcaggcgtt ggcacaccaa
    11881 gccatggcca cccggattct tctttcctca gtatgctttc caaagaccgc aaaatgcttc
    11941 agtcctgccg ggaaaggaat acccatcagc agcccgctcg cactaaccac caaccaggcc
    12001 cacagcagcc gtataatcca ttcttcgccg gacaattccc tgagcaagtc cgggaggttt
    12061 cggaagcaga agaacaaacc gaacagcagc agcaaaaaat aaaatcgtat tccggttctc
    12121 gagaacctac tgctgaggaa agaaccgagt cctgaagccg ttagcagtcc actgagcacc
    12181 agggcgaagg aatataccgg ctgtcccaga aatggggtaa gtttttccag caatgcgatt
    12241 tccaccagca tgaaggccaa accgataccg gcaaaatata gtaaaaccga aatggtgttt
    12301 tccatcctac gcaggtgggt cagaatcgga atgccaatca gcagcagcgc actcaggctc
    12361 actgcggcaa acagcagggg aacaagcagg ccggcttcaa ccagaccttc ccacttgccg
    12421 ccgagactct ttcgaatatc gtccagcctg ttccagtcca ggaagagttc aaaatatggc
    12481 cggtcatcgg taaccggttg caggtcaaat ggagtccttg catgaaaagc tggaggatcc
    12541 gccagcagtt cccgcactcc tagtgcataa actggttcct gcagctggat tacactattc
    12601 atttctgttt caggcatgtc cggataataa actggagcga accctctgct gaggcagaat
    12661 tcccggaaac gactgctttc tttaggtgtc caggcttccc gtgaaaccag caccatcaag
    12721 gtactgagtg tccggaacac tcccagcctc tggtccggtt tccaaccctg acggcgcatt
    12781 gcagtgataa cggtggccag cagccgcatc tcaccgcgag gaggcggaag cagaaaacgg
    12841 tgcacagcca gccaaccgcc gggtgccagg cggttcagca gggactgcat accttcaagg
    12901 ctcatcaact gatcggtttt taatggatcc atgcctgtgc tgccgaccgg ggcagagctt
    12961 tcgattgcaa ccagaatcct gtcaaaattc tgctccggct tttgacaggg ttccggacag
    13021 tcttctgcca ggagagagcg tgcttcagtg gcccgaaatt caatttgcgg gaaacggctg
    13081 tcttcagcca gcctttctgt cagcaacggg tacggcgtct gaaccagaac agaagaagta
    13141 ccggcttcaa ctgcagctag cgcctgctgc cccccaagtg ttttcagtac cagcacattt
    13201 tccggtggtg attgtttaaa atacagtacc cactctggaa ggtgacgcag ataagaacca
    13261 agtgaatcag gaacaagggg cgcaacaccg gtaagaccgt cagcgtccag cgtcaatcca
    13321 agctgatccg gaagcgatcc ccgaaattcc agactcaagc cgggtgcaaa gcgggccagc
    13381 ggagtttcca gccagtcaat ccgtacggaa gcatcgcgct gtgtttccag cagtttgctg
    13441 ccgggatagc gtaaagcatg gggaagattc ttgtagtcgc tgattctgag ttccagtttc
    13501 cccagccaaa atgaaccgca gaacagggtc agcagagttg ccaaagcgag tatattttgt
    13561 ttctttcccg aagccaggca gaggctgctg catgctgcca gaagcgcggg tatacctaga
    13621 ccttcaattt ccgtcaacca cggggccgtc aggaaaaaac ccgcgcatcc gcatgctgcg
    13681 ccgataagat cgcttgcgta aagaaaatgc gcgtgttcgg tgtatgcttc caacagcagg
    13741 ttcagcgtca gtcccgccag aaaaaaaggg aacgccagca gcaggaaaat cagcagcagc
    13801 cagactccgt gccgtggatc cagcgccatc agtgccggat cgaatggcag gtgactgctg
    13861 agcaggaaaa gagggatcag aagcagcgaa aaacagagga caggaaggag aggcgggatg
    13921 cgacgggagc tggacaaacg aaaagccccg gccgcaccgt agcccagcag agctccgctg
    13981 atgatcagga atgcataatg attccacagt tcaatggcca gcagacgcga aagactgatt
    14041 tcgtacatca gagctgccga tgaaaccaga aacatgcctg ctggaatacg tactctcatc
    14101 ttctctgaca aatgtagatc tgaacatttc tgaacccttc gctcatctcc aggtttctct
    14161 ttgcggcagc aattatgcgg gcattggagt tttcacccct agcggctcaa tcgattttca
    14221 aaacctttgc ccccctgatt ctcctcgtct tgagttcaac caaagcctcg tttgcatctt
    14281 ctaatgcgaa ttcctgaatt tcgggtttga ttcgcatttc ggccgccagt gccagaaact
    14341 cactcacgtc ttttcgacat atgttggcaa cacttttgat ttgcttttcc agccataaat
    14401 ggacgggata gtccaggccc agaagaaatt ccttatccgc ctcttctttg ccaatcgcgt
    14461 tgatgaccag tctccctcct ggttcaagat ttttcaaaga ttcaattaca gataaccatg
    14521 ccggtgtggt gtctattatg cagtccagtt tttccggtga gtcctcttca atatcccctg
    14581 accaaacagc gccaagttta ccggaaaact ctctctcctt cacacttctg gcaaagacaa
    14641 aaacttttgt attcggatac tgatgtttca ccatcttcag aaccaggtga ccggaagccc
    14701 caaatccggt aagacccaga ttctgtccgt ccttgagacc ggacaatctc agggatctat
    14761 aaccaattgc accggcacac agcaggggag ctgcctcaga gtcgttaaaa aagtggggaa
    14821 tgtgataggc aaagccttct gctactttca tatactgggc atatcctcca tttgcatctc
    14881 taccggcagc tttgaaatcc ggacatagat tttcattgcc tgccagacag aacttgcatg
    14941 ttccacagac agagaaaatc caggcgatac cgacacgatc tccaattctg aaggtgttag
    15001 cttcgcttcc ggttgcttcg atccggccaa ccacttgatg tcccagtaca attggcaaac
    15061 gcggcgggtg tgttcgcccc tcaatctcat ccaactcggt atggcaaacc ccgcaggcag
    15121 aaaccctgat caaaatctca ttcgctgatg ggatgggatc tggcagttct gtcagttcca
    15181 acggagcctg attttcttcg agtccgcaga gttcattcaa gaccattgct ttcatttttt
    15241 ttcctggctg cgcagagcgg tctgcataga aaggtaaccg gtgaggatgc cgtcctcgta
    15301 gttgaaaaga gcgtctgtgg atagttccgg aaaatcctgg gggttgcgga tgtcttccgg
    15361 agttgcatcc gctacaagtt cgcgtgcaac tttgagaact ttggaccgct gctgatcaac
    15421 catttcctga atcagttgtg caattgagga tgtgtcactc ggcatttctg catttcgaat
    15481 tttgttaatt ttccatcccg tctttggtca taatccgcag tttagcacgc aacttgagta
    15541 ttgtttttga gcggatttgt gataccctgg actctattac acccagaatt tctcctattt
    15601 ccttcaagtt catttcttct tcataataca gagccagcac catctgttcc cgttcctcca
    15661 gttgaccaat tgcttcagcc aatttggttt tcatctgctg caatgacagt atggaaaagg
    15721 gatctttttc tccaggttta gacaggattt ccagtaaact gacactgtct tcctcattgg
    15781 agtaaccttt gaattcttca atggatatta aaggggtggg gctggccttg cggatgaatt
    15841 cgtaatattc atccatactc attttcatct gccctgccat ttcctggtca ctgggttcac
    15901 ggccaagttc aaaagacagt tccctccatg cagagctgtg tttattggtg tcggcacgaa
    15961 ccgaacgcgg cacccagtcc attttacgca gttcatcaag aattgcccct ttgatgcgga
    16021 attcagcgta tgttttaaac ttaacatctc tggtgggatc gaacttatcc actgcatcca
    16081 agagcccaat cataccaact tgatacagtt cttcctggtc tactcctggt ggaaaacggg
    16141 cggccattct agtcacaact cttttgacaa gcggggcatg ttttatgagt tgatcttccc
    16201 tgctctgctg agttttttca gtatagggat tttgcttggt gggcatgata tttactcatt
    16261 cctcaggttc cggaaatgtt cagcagtaaa tctaccatac gttcctttga ggccacctca
    16321 atatcttctg gaactttctg acctactgtc aaataggaaa gcggtttttt gaaacgaatt
    16381 gcatggttga aaagcgaacc gtaggaagca ctttcatcaa gttttgtaaa aataatgctg
    16441 tcaattggca tgcagccgaa tttccttgtt acttcactca tgtcactgtc tttgcttgtt
    16501 gcactcagaa ccagggcatt gtgtatccgt ccgcgttcat caaatatttc acgcatttca
    16561 tacaattgct tttcgttacg ttgagagcag ccatcagtgt caataatgat tgaatcataa
    16621 ctctcatagc tgtcaataat gcggttcatt tcagctttat atttaacaac actgaacggt
    16681 gccttgattt ttttagcaaa agagcgaatt tgttcagctg cagaactatg gtttgtatca
    16741 acagaaatca aggcaacttt tcgtttgtat ttcagcattt gttctgaggc tatctttgct
    16801 aaagtagttg ttttaccaac tccggtcggt ccaagcagcg caactatttt ttgcggtgat
    16861 ttgaggcctt ccatacctct ggtaattttg atgattttca tcagcattcg ggcaatgaaa
    16921 atttttacat atgcaaaatt ttctatatcc tctgtagtca tgtttagctg tgcttccata
    16981 accaggcgtc tcgaaaatct ttcttccatg ccgctgaatt tcatttgctg ataaagcagc
    17041 agcagatttt caggcagatc cggctctgta atgctgcctg tatgggtagc caaaatgtgc
    17101 atcatgtgcc gcatttctcc aagctcgtgt tttaattcac ccatcaaatg cttgtctaaa
    17161 ccgtcagact gactgctcaa atctcctaaa gtaatcctga ttccctgaag ttctttttga
    17221 attgggagca tcatcttccg tgtttcatct ctaactgaaa gtacagattt gttacgtggt
    17281 gtaagaaaag tatcagccaa aactgtttct gaagtttcag agctgccgcc gtatgtctca
    17341 agtgccttcg tggcaggaac tgaggacctg ttgtcctttt tcttttcact ggcggaggca
    17401 gtaacttcca gcatagtttt tccgaacaat ccaaaagcac ccgtgttctc tttaacttga
    17461 cgggttgaaa caatcatcgc gtcagatccc aggtccctct tgatgaattt cagagcctct
    17521 ttgatattat ttacgcggta acgcttcaga ttcataagat tgccagatta caaattttgt
    17581 tttaaattta attttttcat ccctgaagac ctgaaactat gcttaattat tcttgttcta
    17641 accactcaac tctacgacac caagagcttc tatacggaca tcctgagcca cctcattatg
    17701 agagagtacc acaagattag gaatgaaacg ttccacaagt tttttaacat gagggcgtat
    17761 cagcggtgaa caaagcagaa gaggctgata attgttgatg gtaaattgtt ctatcatccc
    17821 atcgatacca tttataatcg cctgtgccat gtttggattc aagccaagat atgcaccctg
    17881 tccggaatcc tgaattgcag tagcaatttg attctctaaa tcctggttca ttgtaatcaa
    17941 aggcagcaaa ccgtccggag tttgatattg tctggtaatc tgccgtgaaa gactctggcg
    18001 gacatgttcg gtcagcaaat caggatcttt agtaatcaga gaaacatctg caagagtctc
    18061 gagcacagta tgaaggtcgc ggatagaaac aagttctttg agcaaattct gcaatacttt
    18121 ctgcaccact cccaatgata gcagtgatgg gatcaattct tcaacaactt ttggttcaga
    18181 ttcagcatga gagtcaatta atttctgagt ttcctgacga ccaataaatt caaaagcatg
    18241 attcttcagg acttccgtca aatgagttgc caaaacagta ggcaggtcaa caactgtata
    18301 gccggcaaat tgggcgcgct cctcatcttc ttctgcaatc cagattgccg gtaatccaaa
    18361 tgccggttcc actgtatcca cacctggaat ctcctcatct acatctccgg ttttcatggc
    18421 aaggtgatgt cccatcatca attcattgcg ggcaatctca attcctttta tcgtaattga
    18481 atattctccg ggagatagtt caagattgtc tttgatgtgc agcggaggaa cgatgtagcc
    18541 gaaatccaat gctatttgac gacgtatagc cttgatccgt ttaagcagtt caccatcctg
    18601 ctcttcatca acaagcggga tcataccgta acccagttcc aggccaagta tatcaatcgg
    18661 tagaagtgat tcgatatctt ccggttcttc acttatttct ttctggagac gtacttgttc
    18721 ttcctgatgt tcatcctctg caatttgcac atttgaacgt ttacttactt gtgccagacc
    18781 gaaagtgaga atcgcgagca gcataaatgg aactataggc agacccggaa ttaacgaaaa
    18841 agagaccaac accgcagata caacatagag cgcctgaaca ttactgaaaa tctgtgtatg
    18901 caaatccaca gatattttat tatcaccgga agctttagtg accgcaatac cggccgcaat
    18961 ggaaataatc agtgcaggta tttgtgagac aagtccatca ccaatggtta aagttgcata
    19021 agaatcaaat gcgtctccgg cagacatgtc gaactgggtg actccaacca gcagaccacc
    19081 taaaatatta attgaggtta ttatcaggcc agcaattgtg tcgccacgca caaacttgct
    19141 tgcaccgtca agggcaccaa aataatctga ctcacgctgg atggttgaac ggcgttgacg
    19201 ggcttcgtct tcatcgatca agcctgcgtt taaatccgca tcaatcgcca tctgctttcc
    19261 aggcatggca tccaaagtaa aacgtgcggc aacttccgca atacgacctg taccttttgt
    19321 gataacaaca aagttaatga taaccagaat caggaagatg atgattccga caatttgatt
    19381 cccgccgact acaaattctc caaaagaatt gattatcgtt cctgcggcac ccggtccctg
    19441 atggccttca agcaggatta gcctcgtaga agctacattg agagaaagac ggaaaatagt
    19501 tgcaattagc agtactgaag ggaaagagga aaactcaaga gggcttttgg taaataacgc
    19561 tacaaaaaga atgagtacac cgacagtgat gcttaaagca aggagtagat ccagtattac
    19621 aagtggcaga ggaaccacca ttatcagaag tatcgacatc acgcctgcag cgagaacgac
    19681 atctgactta ctgactatat tatttagggg aatgcttgct gcggccaccg gtcatccatt
    19741 accaagaagg tcgttaaaat tactgcgtct caaaactgaa acgctcggat gtgcctgttg
    19801 caaatactgc tccaaaaaca aatattgaaa aatattctac ttaacctggg tgaatcagtt
    19861 ttgaggggag ctggactgct aaaactttat ctcttccgga ggcggtaaac gaaggccaga
    19921 atttctgcaa ttgctttata aaatcgttcc gggatatcat cgccgatttc tacatttgca
    19981 tataaatcgc gggctagttc cggtcgttca aggatgggaa cattattttc acgggcaact
    20041 gtcctcattc gcagggctaa atggtcagcc ccttttgcaa tcacttctgg agctgtcatg
    20101 acatccctgt cataaaggat ggctacagaa aagtgggttg gattgacgat caccgcatct
    20161 gctttcggaa cttcctgcat cattcttgcg ttggacatct cgcgctgaat ctgacgtatt
    20221 cttgctttaa gctgcgggtc tccttctgtt tgtttggttt catctttgac ttcctgtttt
    20281 gtcatcatca attgttgctc atgatgccag cgttggtaaa aataatcaaa aatggcaatt
    20341 gccacaagcg ccagaactat cttgcctgca acttctgcta ccacagtaaa attgtatttg
    20401 agaatggact ctggagttga aacagaaagt ccatttaatt cagtgatttt ttcaatgtag
    20461 gttaaatatc cgacaaaacc tactatgatc agttttgcca tactcttgag aaaatctgcc
    20521 agtgactgtg acgaaaaaag tcgtttgagg ccagtcaatg gacttatttt gttaaacttg
    20581 ggcgcaattg ctttgaaggt aatcaggact ccgacctgca gaacggaagc aaatattcca
    20641 acaaatataa ttactgctgc aaaaggtgca aatgcaggaa gaattatgga aagaatttct
    20701 cccgataatg taaaaatact gtcgattgat aattcttcac tttgcgccag gtcccgaaaa
    20761 atttgggaca ttagttgccg catggcatca agaattatgg gactggtggc aacaagggtc
    20821 agcacgattc cggcaagcaa agctgcagaa gacagttcct tgctaaaggc tacttgtcct
    20881 tcttctctcg attccctgcg ctttttttcc gttggaactt cagttttttc ctgaccttct
    20941 tgttctgcca tttgtcttca agaatctgat gctgttaact gtaatgttta atacaaaacg
    21001 aatgtgcatt gggtcgttct accagaaaag cactgttgta atttcattat atcctaatca
    21061 accacccagc aacttgatgg cctcaaaaaa ttgtgtgtca agcttctcaa acatttgatg
    21121 tactgcctgt gcaaaaaagg gcattccgaa aaacagaaac agaaaaccaa gcattaatgt
    21181 caggggaaaa ccgactacaa atacttgaat ttgcggcact gaacgtgcca gaagtccaac
    21241 aacggcattt gcgagaaaaa gagccacaat cagaggtgct ccgatctgca atccgataac
    21301 aaaaacactt gcagaaagtt caattagatt ttcaactaaa ggcctgctga tacttgcacc
    21361 tcccggaggg agaaccgagt agctcctgac caaagcttga agaacaatca ggtgaccgtc
    21421 cattgctaaa aatattagtg tagcagttgt attttcaaat cttccaacta gagatatttg
    21481 ctcctgtgac tgcgggtcga agacattggc catacccagt cccatttgaa atccaatgac
    21541 tgttcctgca aattcgactg ctccaaacaa aaatcgggca attatgccca ggaccagacc
    21601 aatcaacatt tcgctggcaa tgagcataat gtaatgatct ggacgatccg gataaacagt
    21661 gatcagtgga ataaaaggat agagaatcag tgccagcatg agactcagaa ccaccttgat
    21721 ttggggtgga atatttgaac tgccaaagac cggtgcagta gagattattc cactgactcg
    21781 taccagtacc agaataaaag ccagtagctg actggcatca taactcagca gattggccat
    21841 aaagtaaagg aattaacaag ccgcaaaaca cggcacttgt tacataggat tattattaat
    21901 agacacataa aaaacgggat tctgcatccc tttttaaggt gttcaggcag gttttatttg
    21961 acagtttatt cgataaactc agagcactga gtgcatagag aaatcaatca tcaactaata
    22021 tttgactgga gtaatctgca cgggaaaatg tattctgcgc aagtcggctg cgttcttcat
    22081 ttgactgaca attgatacac attcttgcca ggggaacagc cagcagccgt ttcttggaaa
    22141 ttgcctcgcc gcattcgtcg cagtatccga agtcatctga ttcaattcgt ttaagggcaa
    22201 tctcaattcc tgtaagtctt gcctgttctc tatcctgcag taacagatcc aactcccgtt
    22261 tctgttcttc tacagaagaa tcgatggcgt cgccaatatc cttatttatt tcggttttat
    22321 tttctgtttt ctcctgaatc ccggagagta gtgccgcctt ctcttccaat aacaggcgct
    22381 ttatatcttc catgacagct taattatttg ttagtattac atgttggggt aaacaccatt
    22441 attattacgt acagtcaaat ttaagcaaat tctttctgca aaatcaaatc caattaaagc
    22501 ttttctgaag ataaagcaaa aaaatatttt ttgtatgaac aaaaccagac cataattaaa
    22561 cctccggaca actgttcctg ccggcaatac actggttcaa aaggcttctt ttgcaaaaat
    22621 tattataatt aaatgcgtga gtaaaaaacc ggcttttgca ttgcacttgc gccagaaaaa
    22681 acaacttgtc ataataatga ctcactgtgt ttggagaatt ttgtgaaaat gatgtttaat
    22741 tattgtttaa attaagtatc gtgtcctctt aattattcaa caggattttg ataaaatgaa
    22801 acaattagga tcaggtatag aatagtgaga agacttgcgg aaaattgtaa tatttgcggt
    22861 aaagtgattt cacgtttcca ggcacataaa atgatttgcc agcgctgcaa agaagttgtt
    22921 tgccgccaat gccttgtgga tgtatcacat ataaaaacca attggaatat ttgtcgaaaa
    22981 tgtgaaggag cttataactg atgcagcaga taaataataa gtgcagtaaa tcaattttta
    23041 tttgttgcct ttacatgact ggatttctgc tgactgaaac tgcaattttc ggtcaggaaa
    23101 gctacctcag tgaattgctg ccgggtaccg agtcttcatt tcagcacttg aatttggaga
    23161 gttctaaaat aacagggaag agccatttcc gctacgaaac attgataaag gaaggaaccc
    23221 ggttcgttgt tgaaaaaaat gaaaacacaa agactgatgg cgaagttttt acccgaaaga
    23281 cgctctggtt tgatgcagat tccggtattc caaaatggta tgaggaagag gatttgcgaa
    23341 aggagttccg gataacaaac agctataccg gacaaatcat gcacaccaga ctagtagaag
    23401 acgggcagat tctggagttt aaaacaaact tgagcgccga aaatgcggtt ccttttgagg
    23461 tcgttatttt ttttctgcga aaatatttaa aacaaattct gcagaccaaa aatttttcct
    23521 tcaccctttt tatcccgctg ctggccattg agttggaaaa aaaaggactc ccacgttcaa
    23581 tgagcatgat cagcatgtgg gctgaaccac aggaaaaaat cagaatggac actccgcttg
    23641 gagtcatgaa tgcacacacg attctggttt caccgcaatc cggattgtta cgcgctcttc
    23701 tgcctcggga aaaaactcat tttgaattta cctttgcagc agcctctccc taccatctgc
    23761 tgcagttcaa agcaggaaaa accagacacg ttctgaccag tctcattctg ccagaataat
    23821 cttgaacaga aaatctctgc ggtcccgaaa attgcagaaa ttctgtcatc aatttttgga
    23881 gagcaggctg gcggttaatc tgtatattct gcttcaagtg aacttcctgc gtcaataagg
    23941 tgctgcattt tacgtctcca gacaattccc tcgctggccc agcggatgac aacccggaca
    24001 aattctcgac caaaaccgag cttacggcca atgtgcagaa gatagtcgat ttcgtttgca
    24061 ccaaggacat tgtcaacaga aataactgag gtcaggtcga tgaatatttt cacttccatt
    24121 tctctcgttg caccgggaaa acggtctaat tccggaagct tttggtcttt cacggcccgc
    24181 atcatgttat tcacttctgt ctcagagggt aaaaaagcga gtgcccgttc aaggtattca
    24241 agctcctcgg gagcaactga accatcggat gttattgcac cgcagatcgc ataggcaagc
    24301 cactgcttct ggtcgtcttc cagcagttcc ggttgtaaac cggatgttaa ttgaaattcc
    24361 attttttcct tagctgactt aaaatgaaga gtagaattct tttcactaac cttattaact
    24421 gagttttccg caaagcgcaa gaaaatgagt ttaaaattcc tgattttttc tcattgaaga
    24481 acaagttaat gttaattatt ttattctcag accataatta aatgctgaaa acagcatatc
    24541 ccgtcctgac agaaactgct ctttcattga tgttctgaaa gatctggttt agaatgaatt
    24601 ctgctgcaag gtgatattcc tgtgcaaatt cctgttttga gatcaaatat aaaaattatt
    24661 atttcctcat gtttcaatct gtttacactg agaacatact tattttcctg caaaaataag
    24721 tatgaaccaa gtatctgttt acgttcttga catcagcgtg ctgctttgta caccgggagc
    24781 tcttttcgag tttccggata aagagattgt aattccagtc acaattcttg aagaactgga
    24841 ttcactgaag ctggatttgg gagaaaaagg gcgctctgct caaattgtca gtcaaatgtt
    24901 agatgaatgc cggcaatatg gtagtttagt agaaggtatt tccctgccga atggaggaaa
    24961 attacgaatt gagcttacag aaccagaatc gggtttgctg ccgtacagcc tcaatttgaa
    25021 acgaatttcc aacagagtcc tggctgttgc ctggatgttg agtcaaaaaa acaaggatct
    25081 tatccttgtt tcacaggacg aaaatttacg aacaaaagct aacacactca atgttccaac
    25141 actttcatac aatgggcaaa gaccgaatga ctcaaacctg tatgctggaa ttcgtcaaag
    25201 tgaggtcagc aaacaaaaat taaggagcct cggtaaacag tcttatattt ctccggaaga
    25261 ggttttttca gatcaaaatg aaatttgtga attttatcca aatgaaggcc ttcttttaag
    25321 ctgcaccgat gttccagatg aacagatatt agctacatat cagcagggta agaagaagtt
    25381 cgaactggtt cccaaagaac agggtgtttg gggcatcagg cctctgaatc ctgaacagcg
    25441 ccttgcactg gccttgctga tgaatccaaa gatttctgtg gtaacactga gtggaatatc
    25501 tggaactggg aaaactttac tggctcttgc tgtcggtttg cagcaattaa tggtggacaa
    25561 tatttactca cgtatgctgg tttcacgccc tatattccca atgggacgtg acctgggtta
    25621 tctaccaggt gatactcagg aaaaacttgc tccgtggatg cagccgattt ttgacaattt
    25681 ggaattactg atcaataatc ctgcttcaaa aaatggttca aagcatgacc gttacaatga
    25741 gttgatggat cgtggaatgc tggttgttga gccgctgact tatatccggg gacgtacaat
    25801 tccaaatcag tacatgattg ttgacgaagc ccaaaatttg actccacatg aaatgaagac
    25861 gatattaaca cgtgttggtg aaggaactaa aatcgtttta acaggtgatc caaaccaaat
    25921 agacaattca gaagttaatc tttcttccaa cggactaagc acactggttg agcgtttcaa
    25981 agaatccccg ttggcaggac atgtacgctt tacaagtgtt gaacgctcac cgctggctga
    26041 attggcggca actgttttgt gaataatctg gaatctgaaa atctggttaa gaaaaaaatg
    26101 aatatgcgaa ttttgaattt tctttagatt ttcatggaca aaacacttaa taaatccatc
    26161 tgcttttgtt attattggta actgaaactt taaataagaa acttgtaact ttaaatcttt
    26221 catgtcatta aactggaatt ttgaacctcc tcctccttct gaatttgcag accgtgaagt
    26281 ccgtttaacc ggttctcatt tatccggaaa gcaaattgct ttgctgataa ccggaagcat
    26341 agctgcttat cgaatgccgg atctgattcg tgatttccgc agggaaggtg cggatgtagt
    26401 tgtttatgca acgactgaag gattacgtta tgttgccagg gaggctcttg aatggtgttc
    26461 acaaaatccc gtcattgaag gctttacttc ggatgcagaa catttgagtg actccacacc
    26521 tttcgatgcc ttcgttgttg ctcctgcttc gtacaacaca ttgaataagg cgtctttggg
    26581 aattgcagat tctgtggtca ctgcagcaat tgctgcagcc ctgggtagaa tggaacgaca
    26641 gggaattcct gtcctgtttg ccccggcaat gcacggtgca atgcataaca gtatcttaac
    26701 ccgttcaatg cggactttgc aggaaatggg cgttaccttg attccaccgc accaatctca
    26761 tggaaaaaac aatcttgctc cgctggagct aattgtagct acaacaatcc gggcactcag
    26821 cgagtcgccg cttcgcggaa aatctttgct gatcaccggg gggccaactc ctgttccggt
    26881 ggataatatc agaaggatca cgacacgttt tacaggtgct ctttccatcc aattggcccg
    26941 tgaagcatgg atgcgcggtg cggcagttga attgattctc ggcaagggaa gccgctctgc
    27001 tccggaatat ataaaagcaa aaaccgcaga aacttttgag gattatagaa gaaccttaaa
    27061 agaatcgctg caggaaggaa actatgattg gggaattttt acagctgcag ttgcagattt
    27121 tcagccggaa tcagtttttg aaggaaaact tcccagtaat cagaaacagt taagtttgaa
    27181 actggtaccg actgtaaagg tgattgaaga agttcgtcag catttccctg aactgaaaat
    27241 ggtcaccttc aagtatgaag aaaatatttc tcatgaagaa ctgatggaga ttgcaaaaaa
    27301 ccgtttgtct gagaagggcg gttctcaact ggttgtggct aaccgtgtag aagaattcaa
    27361 acaggatgga acacaggtgg catggctgct ggaatcaaaa caggaacctc aaaaacatat
    27421 tggcaaaaaa gatattgcaa acgcaatcct tgaccgcatt gaacttactg catgaaactt
    27481 ttgatgtaag attctgtatt ttcctgcgac ctaaaatata cggcaatttg ttttaacaat
    27541 tcaaacctaa atccaactct caacactaaa aacctcatgg tggaccgctg ggccaacaca
    27601 gtcgtgcatc cgacaatgga tgacatagca aattttgtcc cccgccaaat ttctgccaac
    27661 tctattacgt tcattggttt cggaattggg ctcttagctg tacctttgct ttggctaaaa
    27721 ctctatcctg cagcactggt cctgattttg gtcaaccgtt ttttcgatgg acttgacggt
    27781 gcagttgccc gcagaaacgg tgttactaat ttaggtggat accttgatat cacctgtgat
    27841 tttatttttt attctgcagt aattgtaggc tttgcactgg ccagtccgga gaataacagt
    27901 ttgacagcaa cttttttaat tttttcattt atcggcaccg ggacctcttt tctggctttt
    27961 gcagtcattg cagaaaaaca cggaatatca tctgcagcac atggacaaaa agcttttttt
    28021 tatctgggag gtctgactga agggacagaa acgataatat tttatatcat catttgcctg
    28081 tttccggagt actttccaat tctggcagta gtgtttggag gtctctgttg gatcactgtt
    28141 gtcggccgtt ttggttcagc ttattctttg ttgcgctaaa aggaaataac cgaaaccact
    28201 ctgcacttcg ccctgaatga caattcataa taagttaaat ttttacaagc cattcatggt
    28261 ttgacaaaca acttacgggg aacattcgcc agagtttcca caccagaatc tgtgataaca
    28321 atgctttcgg ttgtttccaa tccccaatcg tcaaaccaaa gtgcaggcat aaagtgaaag
    28381 gtcatgtttg gttccaacac cgttttgtcg cctttgcgga aactgaccgt ccgttcgccc
    28441 caatccggag ggtagcttaa tccgatggca tatccgcagc ggctttcttt ttcataacca
    28501 tattttgcaa ttgttttgcg ccaagccgct tcaatatctt ccgcgaagtt gccgggtttt
    28561 gctgcttcca aacctgcttc aattccttcc agaacagcct tttcaacatc taaatacttt
    28621 tgcggcggct ggcccaaata aatggtgcgg gaaagcggac aatgatagcg ccggtaacat
    28681 ccggcaattt caaaaaaagt tccggagttg ttcggaattg gttgatcgtc ccaagtcaag
    28741 tgcggagccg atgcatctga gccggttgga gtcattggta caatcgccgc ataatctccg
    28801 cccgcgtctt ccgcacctgt aattcccgaa tgataaattt ctgctaccaa atcattttta
    28861 cgcatgccgg gttccatcac ttcaaaaata cgtgcgtgca ttttttccac aatcctcgcc
    28921 gctttgcgca tgaattccaa ctctcttgga gatttcacgg tgcgctgcca attgactagt
    28981 ccggttgagt caacaaaatt tgcctgtact aaatttctct gcaaagattc cagacaggat
    29041 gcagaaaaat aataattgtc tttttctact ccaattgttt tttgtgccca gttcttttca
    29101 gtgagaatct cgcttaaata atccatcgga tgtttctcgg gattctgcac ataatcatcc
    29161 ggataaccga tgatattttc gtgctgcatg aacaccgtga gttttgctcc gttggcgtcc
    29221 attccacgtc cataccaaaa cggctcaccc tccaacgaca gtaccacgca ctgatgcaca
    29281 taaaacgacc aaccgtcgta accggtcaac catgccatgt tggacggatc cgtgacgatc
    29341 agtacttcaa tatttttttc atccatactt ttccgcacct ttgaaatgcg gatttcgtat
    29401 tcttcttttt caaatgcggg catattttcc tttgttcaag agtt
//
LOCUS       DTVD01000095            2297 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20196_length_2297_cov_640.927297, whole genome
            shotgun sequence.
ACCESSION   DTVD01000095 DTVD01000000
VERSION     DTVD01000095.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20196_length_2297_cov_640.927297"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            1..1920
                     /locus_tag="EYO68_03510"
     CDS             1..1920
                     /locus_tag="EYO68_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HIC06902.1"
                     /translation="MNFHDEYQQSIEQPEVFWRKQAEQIKWYEFPETILSQDEHGFYR
                     WFAGGKLNTAYLALDVHIEEGRGDQLALVYDSPAANMQQKFTYAELRDTVAKFAGCLR
                     NLGVEKGDRVIIYMPMVAEAVIAMLACARLGAVHSVVFGGFAPNELAVRIDDAKPKVL
                     VTGSCGIEFTKIIEYKPLVDKALEVSKFPPENIVVLQRPQAKATMQSGRDHDWNELMS
                     QASPADPVSLDATDPLYILYTSGTTGLPKGVVRDNGGHAVALKYSMKAVYNTDPGDVY
                     WAGSDVGWVVGHSYIVYAPLLHGCTTIVYEGKPVRTPDAGAFWRMIAEHKINTFFTAP
                     TAFRAVKKEDPEAKLLKQYDISSLRTLFLAGERLDPPTYDWLTEILKEAPQPGIPVVD
                     HWWQTETGWAICSNPMGIEPQPVKNGSPTFPMPGYNVQVLDEVGEICKPGETGNIVIH
                     LPLPPSCLPTLWNDDERFKQSYLSQYPGYYLTGDEGIKDEDGYIFVMGRTDDVINVAG
                     HRLSTGEMEELIAGHPAVAECAVTGIDDELRGQVPVGFVVLKDGIDISQDQMQEELIA
                     IIRREIGAVAYFRQSGIVKRLPKTRSGKILRKTIRRLAIETEVLVPPTIDDPKILDEI
                     REVMQERGIGRYATS"
     gene            1982..>2297
                     /locus_tag="EYO68_03515"
     CDS             1982..>2297
                     /locus_tag="EYO68_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06903.1"
                     /translation="MIQQKALYIFLAKRTVWFWFAGKRSCLKPVSKPLNSENILEHCR
                     KWSASRIILFLEFPWVLTHFDITITLSSEELASFAKMRLSLANKTDTGERGVQAKQSR
                     NSV"
BASE COUNT          630 a          491 c          562 g          614 t
ORIGIN      
        1 atgaattttc atgatgagta tcagcagtct atagaacagc cagaagtttt ctggagaaaa
       61 caagctgaac agataaagtg gtatgaattt ccggaaacaa tcctgtctca ggatgaacat
      121 ggattttacc gctggtttgc cggcggaaag cttaacacag cctaccttgc actcgatgtt
      181 cacatcgagg agggacgcgg tgaccagttg gcactggttt atgattctcc ggcagcaaat
      241 atgcagcaaa aatttactta tgcagagttg cgagatacgg ttgcaaaatt cgccggatgc
      301 ttgagaaact tgggtgtgga aaagggtgac cgtgtcatca tttacatgcc aatggttgcc
      361 gaagcagtga ttgcaatgct tgcttgtgcc agactgggtg cggtccactc tgtggttttc
      421 ggcggttttg ctcccaacga gcttgcggta cggattgatg atgccaagcc aaaagtactg
      481 gtgacaggaa gttgtggaat tgaattcacc aaaatcattg aatacaaacc actggtagac
      541 aaagcactgg aagtttccaa attccctcct gaaaatattg tggttttgca gcgtccacag
      601 gctaaagcaa ctatgcagtc cgggcgggat cacgattgga atgaactgat gagtcaagcc
      661 tctcctgctg atccggtttc tctggatgca acggatccgt tatatattct ctacacttcc
      721 ggaactaccg gtcttcccaa gggggttgta cgtgacaatg gaggacacgc ggtagccttg
      781 aaatacagta tgaaggctgt ctacaatacc gatcccggag atgtttactg ggcaggttca
      841 gatgtgggct gggtagttgg acattcatat attgtttatg ccccgctgct tcatggctgc
      901 actaccatcg tttatgaggg aaagccagta cgtactccag acgccggtgc attctggcga
      961 atgattgctg aacataaaat aaatacattt ttcactgctc ctaccgcttt tcgtgccgta
     1021 aaaaaagaag accctgaagc taaactatta aagcagtatg acatcagttc tctgcgtaca
     1081 cttttccttg ctggtgaaag gttggatccg cctacttatg actggttgac tgaaattttg
     1141 aaggaagctc cacaacctgg tattccagtg gttgaccatt ggtggcaaac cgaaaccggt
     1201 tgggcgattt gttcaaatcc aatgggtata gaaccacagc ctgtgaaaaa cggctcgccg
     1261 acttttccga tgcccggcta caatgtgcag gtgctggacg aagtaggtga gatttgcaaa
     1321 cccggagaaa caggaaatat tgtaattcac ctgccattgc cgccaagttg tctgccaaca
     1381 ttgtggaatg atgatgaacg ctttaaacaa tcttacttgt ctcagtatcc cggatattat
     1441 ctgaccggtg acgaaggaat caaggacgag gacggctaca tttttgtaat gggacgcaca
     1501 gatgatgtaa tcaatgttgc cggacaccgc ctttcaaccg gagaaatgga ggagttgatt
     1561 gcgggacatc cggccgttgc tgaatgtgct gtcactggaa ttgatgatga attacgcggg
     1621 caagttccag ttgggtttgt tgtactcaaa gatggaatag atatatctca ggatcaaatg
     1681 caggaagaac tgatagcaat cattcgtaga gaaattggtg ctgttgccta ctttcgacaa
     1741 tccggtatcg tcaagcgctt gcctaaaacc cgttccggaa aaatcctgcg caaaactatc
     1801 cggcgactgg caattgaaac tgaggtttta gttcctccga caatcgatga tcctaaaatt
     1861 cttgatgaaa ttcgtgaagt aatgcaggaa cgtggaattg gccggtacgc cacatcttga
     1921 cctcaattta gcgccggttt aatgcaatcg aagttttttc ccttccttca cttgagtgca
     1981 gatgatccag caaaaagcgc tatatatttt ccttgcaaaa cggactgtat ggttttggtt
     2041 tgcaggaaaa cggagctgcc tcaaacctgt atcaaagcct cttaattcag aaaatatctt
     2101 ggagcattgc cgtaaatgga gtgcttcgag gattatttta tttcttgaat tcccatgggt
     2161 actcactcat tttgatatca caattacgct ttcatcggag gagcttgcat ccttcgccaa
     2221 aatgcgtttg agcttagcaa ataagacgga cacaggagaa agaggtgtcc aggcaaaaca
     2281 atcccgcaac tcagtca
//
LOCUS       DTVD01000096            9093 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2030_length_9093_cov_217.145497, whole genome
            shotgun sequence.
ACCESSION   DTVD01000096 DTVD01000000
VERSION     DTVD01000096.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_9093_cov_217.145497"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="EYO68_03520"
     CDS             complement(<1..1956)
                     /locus_tag="EYO68_03520"
                     /inference="COORDINATES: protein motif:HMM:PF01464.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06904.1"
                     /translation="MLQSNSSLPAFKISAQNFFYRNRCKTVAEVLIIAAMLFSASSAS
                     AHKISSWEPYKSLFEQGECQKLISHLKPLSKPRDWYDNRMWSRSRILNSKCQMQLENY
                     KAALKSLKQTPESEERDVWLFQKIRVLLKSGNQSKAITNIRKLLKLPEKFSYLQSLRD
                     DLKNEFHTDKEVSLLFPLLHETRKNYKWFLSDYDLHALYIRGAKLKGIKPEHTYRVLG
                     WQFPIDEKTARQSHKNLTAKDLKNMSPAELLKRVRTLTRLGLNDYLVKHLPQLRKWRS
                     RKVLKKLGKAYLKALFVERFYYRIIIFQKKGQLSKLWSLPKETQLYWTARSFIKRRNI
                     PDARSTIYKLERLNAKSKLLPDLFNTFAIRYMLDSEIEKSRFWWNRLLSNFPKHKLAA
                     AAAWKLAWSNLQQNNTKKALSYLKRGLKTRIYNSEMKAKLLYWQGKLQQVTGRPDLAE
                     KSFKKLILHQPNTYYGMRLLSAKDIPGSILAVVKSRKTKLYAEPTEPLSKKTKKLLKR
                     TEFLFDIAEPEQALRELFAGLGRFKNSSRNWHVSHLLHRRGKHHALLRIVANYYLPRM
                     ISLEVGDYPLWELAYPRPYWSKLKSFANQAGIDPYFALAIMREESHFDPQALSSSKAI
                     GLMQLMPATAKAVAKRKKIKLNEKEEIF"
     gene            2098..2835
                     /locus_tag="EYO68_03525"
     CDS             2098..2835
                     /locus_tag="EYO68_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06905.1"
                     /translation="MDNERSRLKESISGKNKKENTTETVNSVPLQPLILKQNPKNIKG
                     TKSLPYDFGAETYDVYSNPKNPFMVGILAAETDKIQLDPIKAQSLFQQLEKKSPYSLE
                     YPLNISSHRKRKLSPATQPISEDADSETETAGSERKLEIASSEYLPREKLKENKRKLD
                     VYIQGFFCHVLKRLSEDPGELAQIINEIVPLLVEHQIRVRFYQAFCQIRSSGDLNTTL
                     EKISKDVELENFELFCSALFVFYSSQK"
     gene            2890..3999
                     /gene="alr"
                     /locus_tag="EYO68_03530"
     CDS             2890..3999
                     /gene="alr"
                     /locus_tag="EYO68_03530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIC06906.1"
                     /translation="MRPTFIEVNLDYLRYNLEQIRKHTQNRPVMAVVKSNAYGHGMLR
                     VAQLYEKLGVNCLGVALLEEGIILRESGISLPIVVFGGVLLKQIPEYLKWNLEFFISS
                     LNVLRKTDKICESSGQQAIVHLKLDSGMGRVGTIAEESAGFIEEAVRARHLTVKGVCS
                     HLACADDPEDPMTLEQVDRFLTAVSIFERLDAPVPLRHLANSGGVLYFPQSHLDLIRP
                     GILLYGVYPDSSSPHVLDVRPALQLKSSVSFHKSVPKGFSVSYGSTWTSDGSTEISTI
                     PLGYGDGFRRHLSNHGEVIIRGKRRAIVGRVCMDQFMVNSNNETLEVGDEVVLIGEQD
                     GQSITVEEMSGWAETIPYEILTNLNDRIPRIYSNG"
     gene            complement(4286..4504)
                     /locus_tag="EYO68_03535"
     CDS             complement(4286..4504)
                     /locus_tag="EYO68_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06907.1"
                     /translation="MRKSTMNCRWIWIILMPIWLAGSCETRLTTFYVKAGDSEELKIH
                     AFERCGRHYRVLAYEDDTARIECLKKTD"
     gene            4991..5692
                     /locus_tag="EYO68_03540"
     CDS             4991..5692
                     /locus_tag="EYO68_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIC06908.1"
                     /translation="MDSRNMTQNQLSASIRSKKGKSANQKLRQAGNIPAVLYGPRGNI
                     LLEMAEESTRHLLEKMSGMHELVPINVTDSASGESWTAQVLLREIQKHPYQHQITHID
                     FWELPEAKPQIVRIPIHVIGESPGVKGGGVLQMVVREIPVSCLPADIPSFVEVDSSSL
                     ELGDSIRIQDVKLPEKVTVGTDENFAVISIVGRAKEEEEEIVEVAEGEEGVEAVEGEE
                     ADSEDSVKDEKTEEE"
     gene            5718..6296
                     /locus_tag="EYO68_03545"
     CDS             5718..6296
                     /locus_tag="EYO68_03545"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIC06909.1"
                     /translation="MAGMKLVAGLGNPGREYEGTPHNVGFQVVDLLMEKLEVSGFQQK
                     FHSQFCRTSIEGEACILIKPQTFMNRVGLAVAECAGFYKIPAENIVIISDDLDLPPGM
                     ARFRDGGGHGGHNGLRSVIDSLGNSKFRRVRFGIGRPAGKRDVVGYVLGQWSKAEAKL
                     AQSVMDLVLVELIGFLATSQFENTSFSAPELA"
     gene            6345..7436
                     /gene="ychF"
                     /locus_tag="EYO68_03550"
     CDS             6345..7436
                     /gene="ychF"
                     /locus_tag="EYO68_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIC06910.1"
                     /translation="MALEVGILGLPNVGKSTLFRALTKVQVEAANYPFCTIDPNIGIV
                     RIRDPRMIRLTELVSPEKIIPATLQIVDIAGLVKGASHGEGLGNQFLSHIRQVDAVAH
                     VVRCFEDENVVHVDGRLDPVGDVQVIQTELILSDLEQVERKIDRLQRQVKGDRALAVE
                     LGIFERLRDHLDDGLLASKMGLSKDEKELINELSLLTLKPYLYIANISEDGLNNPEVR
                     EQQLRELADEEGVEVIPICAQVESELSEMDDDESKLFMEELGIDSSGLDRLTHSGYRM
                     LEQITYFTAGKQEVRAWEIRKGSTAPEAAGKIHTDFEKGFIRAEVFHFDDIDQLGSES
                     SVKDAGRWRLEGRDYVVQDGDIMHFRFNV"
     gene            7546..8097
                     /gene="rpsF"
                     /locus_tag="EYO68_03555"
     CDS             7546..8097
                     /gene="rpsF"
                     /locus_tag="EYO68_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIC06911.1"
                     /translation="MTGYELVFITDPSHNNDELASVLKNYKKILTKSGGKLIHEYVWG
                     RRRLAYEISGNDFGVYHAWYFTGTGKTIDELQRQLGYSDDVLRHQIIKTDDIDTEAAF
                     LQTLIPPKEDASENEETPKESEGITGESTKVKKESPKKAEAATENEKNVTEDVKEVDT
                     ESEVKIEKSDKDAEEVETESVDA"
     gene            8155..8493
                     /gene="rpsR"
                     /locus_tag="EYO68_03560"
     CDS             8155..8493
                     /gene="rpsR"
                     /locus_tag="EYO68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIC06912.1"
                     /translation="MLKKRKRSHLRRKRIPTSILLRSKRKSCPFKEAGIDRIDYKDVE
                     LLKNYVTQGGKIIPSRISGVCAANQRKLKTAIRRARNLALLSPMKGYVPQQVNVERKF
                     SSYRNSKPPE"
     gene            8554..>9093
                     /locus_tag="EYO68_03565"
     CDS             8554..>9093
                     /locus_tag="EYO68_03565"
                     /inference="COORDINATES: protein motif:HMM:PRK00137.2-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIC06913.1"
                     /translation="MKLILTEDVPNLGSLGDTIDVKSGFGRNYLLPKGIALLATGRVS
                     KELQHRIQHLNKLREGKIELAHEQAEKLKTVKLEVVRKSGPGGRLFGSVTNRDLKELL
                     KEKEFDVERRSIQLNSPIRNIGLHEFTVRVHSEVSVNMQIKVTGDSDESAVKTAAVDE
                     PKDKNADEKNVDSEELEKLE"
BASE COUNT         2722 a         1646 c         2074 g         2651 t
ORIGIN      
        1 gaagatttct tctttttcat tgagtttaat tttctttctt ttagctacag cttttgcggt
       61 ggcgggcatc aactgcatta aacctattgc cttggatgaa gataatgcct gaggatcaaa
      121 gtgactttcc tcgcgcatta ttgccagagc gaaatatgga tcaattccgg cttgatttgc
      181 aaagctttta agcttggacc agtaaggtct cggataggcc agttcccaga gcgggtaatc
      241 cccaacttca agtgaaatca tgcggggcag ataataattt gcaacgattc tgagcaatgc
      301 gtggtgcttt cctctgcggt gaagcaaatg actcacatgc cagttccgtg aactgttttt
      361 aaaacgcccc aatccggcaa ataattctct caaagcctgt tcaggttctg caatatcaaa
      421 gagaaactcg gttcttttca gtaatttttt ggttttttta ctgagtggtt cagttggttc
      481 ggcgtatagt tttgttttgc gggacttgac tactgccaat atgcttcccg gaatatcttt
      541 tgcggaaagc aggcgcattc cataataagt gttaggctgg tgaagaataa gttttttgaa
      601 gcttttttca gcaagatccg gacggcctgt cacttgttgt aatttaccct gccagtacag
      661 taacttagct ttcatctcag aattgtaaat tcttgtttta agtcctcgtt tcaggtatga
      721 cagggctttt tttgtattat tttgctgcag gtttgaccag gccaatttcc atgcagcagc
      781 agcagcaagc ttatgcttgg ggaaattact cagcagacga ttccaccaaa atcgtgattt
      841 ctcaatttct gagtccagca tgtaacgtat ggcaaatgtg ttaaacaaat caggcaggag
      901 cttggatttc gcattaagtc tttcaagttt atagatagta cttcgtgcat ccggaatgtt
      961 tcttcttttg atgaaggagc gtgccgtcca gtaaagctgg gtttcttttg gcagagacca
     1021 tagttttgaa agttgacctt ttttttgaaa tatgataatt cgataataaa atctttctac
     1081 aaagagtgct tttagatatg ctttaccaag ttttttcagc accttcctgc tgcgccactt
     1141 ccgaagctgc ggcaaatgtt ttaccaggta gtcattaaga ccgagtctgg tcaaggtccg
     1201 tacgcgtttg aggagttctg ccggagacat attttttagg tccttggcag ttaagttctt
     1261 gtgggattgg cgtgcggttt tttcatcaat gggaaactgc cagcccaaaa ctctatacgt
     1321 atgctcaggt tttatcccct tcagttttgc accacggata tagagtgcat gaagatcata
     1381 atccgaaagg aaccatttgt agtttttacg ggtttcatgc agcagaggaa aaagtaagct
     1441 gacttcctta tctgtatgaa attcattctt caaatcatct cgtagcgatt gaagataaga
     1501 aaatttttcc ggcagtttca gtagttttct gatattggta attgctttac tttgattgcc
     1561 agatttaagt aaaaccctga ttttttgaaa tagccaaacg tctctttctt cagattcggg
     1621 agtttgtttt agactcttca gggccgcttt ataattttcc aactgcatct ggcatttgga
     1681 attcaagatc cgtgatcggc tccacattcg gttgtcatac cagtcccggg gctttgacaa
     1741 aggttttagg tgactgatca atttttggca ctcaccctgc tcgaacaaac ttttgtatgg
     1801 ttcccaggaa gatattttat gtgcagatgc agagctggca gaaaataaca tggctgcaat
     1861 gatcaaaact tcggcaactg tcttgcagcg attcctgtag aagaaatttt gtgctgaaat
     1921 tttgaaagcc ggtaaagagg agttactctg caacatgatt tgatacaggt ggtatagttg
     1981 gtgctacttg atagctagga tgcattaagt taattctgcg ctcaggatgc caaaaataca
     2041 agagcaaata tcttgccgta atgcttaaat aagttttata taaaaggaaa taaataaatg
     2101 gataatgaaa gaagccggct gaaggagtcg atttcaggga aaaataaaaa agagaataca
     2161 actgaaacag ttaattctgt tcctcttcag ccactgatat taaagcagaa tccaaaaaac
     2221 attaaaggaa caaaatctct cccttacgat tttggtgcag aaacctatga tgtatacagt
     2281 aatcccaaga atccgtttat ggttgggatt cttgcggcgg aaacagataa aattcaattg
     2341 gatcccataa aagcacaaag tctttttcaa caattagaaa aaaaatcacc atattcgctg
     2401 gaatatcctc taaacatttc ttcacaccgt aagagaaaac tctcacctgc aacacaacct
     2461 atatcagagg atgcagatag tgagactgaa acagctggaa gtgagagaaa attggaaatt
     2521 gcctcctctg aatatttgcc cagagaaaaa ttaaaagaaa acaaacgtaa gctggatgtt
     2581 tatattcagg gttttttctg tcatgttttg aaacgtctca gtgaagatcc tggtgaactt
     2641 gcgcagatta taaatgagat cgtaccatta ttagtggaac accaaattcg agttcgtttc
     2701 tatcaggcat tttgccaaat tcgaagcagt ggtgatttaa acaccaccct cgaaaaaatc
     2761 agcaaagatg ttgaattgga aaattttgaa cttttctgca gtgcactttt tgtcttttac
     2821 agttcccaaa agtgacagca acattgtgta aaaaaatcct ggtttttttc attttaagta
     2881 tctgttttta tgaggcccac cttcattgaa gtcaatctgg attatttaag atataatctc
     2941 gaacagattc gaaaacacac acagaatcgt ccagtgatgg cagtcgtcaa gtcaaatgca
     3001 tacggacatg gaatgctgag agttgcacaa ctctatgaaa aacttggagt aaactgtctg
     3061 ggtgtagccc tgttggaaga aggaatcata ttacgggagt ccggaataag tttgccgatt
     3121 gtggtctttg gcggtgtatt gttaaaacag attccggagt atttgaaatg gaatttagaa
     3181 ttttttattt cttctctgaa tgtattacgc aaaacagata aaatttgtga atcttcaggt
     3241 cagcaggcaa tagtgcatct taagcttgat agcggaatgg ggcgggtcgg aaccattgcg
     3301 gaagagtcag caggttttat tgaagaagca gtacgtgcac ggcatctgac agttaaaggt
     3361 gtgtgttcgc atttagcctg tgcagatgat ccggaagatc cgatgacact ggaacaagtt
     3421 gaccggtttt taacagcagt atcaattttt gagcggcttg atgcaccggt tcctttgcga
     3481 catcttgcta attcaggcgg cgtgctttat ttccctcagt ctcacttgga tctcatccgg
     3541 ccgggaattt tactttatgg agtataccca gattcatcaa gtccgcatgt tctggatgtg
     3601 cgtccggctc tccagcttaa atccagcgtt tcttttcaca aatctgttcc aaaaggattc
     3661 tcagtcagtt atggatcaac ctggacttct gacggatcca cagaaatctc cactattcca
     3721 ctgggttacg gcgatggttt tcggcgtcac ctttcaaatc acggtgaagt aataatccgt
     3781 ggaaaacggc gggctatcgt aggacgggtt tgtatggatc agtttatggt caactccaat
     3841 aatgaaactc ttgaagtagg agatgaggtc gttttgattg gagaacagga cggacagagt
     3901 ataactgtgg aagagatgtc cgggtgggca gaaacaatac catatgaaat tttgacaaat
     3961 ttgaatgatc gtattcccag aatctactct aatggatagt cacaaatatt gacttgcatt
     4021 gatttatatg atgtgctatt ttcgtgtgca cttttgtttt gttgataagt aatttattga
     4081 taagcaaaat gacagaggaa ttaaaaatta aaaagttttg atttttcagt atttaacacg
     4141 ctccaaaaaa aatcattaaa ggagaatggc aatggatggg aatacactgg caacaattat
     4201 tgcgatttta cacataatat gggtcttgcc gttaggatat ttttctgtaa tggatatttt
     4261 ggaacgctga ttatttaaac cctctttaat cagttttttt cagacactct atgcgtgccg
     4321 tgtcgtcctc gtatgcgagt acacggtagt gtctcccgca acgttcaaaa gcgtgaatct
     4381 tgagttcttc agaatctccg gcttttacat aaaatgtagt cagtctggtt tcacaggagc
     4441 ccgccagcca gattggcatc aaaataatcc aaatccatct acagttcata gtacttttcc
     4501 tcatggaaat tctagcgtgc ccatctgtta ttatgggtta aatcaaaagc aacgggatcc
     4561 caggccggta atcgggcttc taattcgtca aaatatgaat ctggcacatt ttcaacaagt
     4621 cttccaaact ccattcggcg gctcaaagaa tgtttaagat aagccatgtc agtgactatt
     4681 cccgcaaaag actcttcccg tacatgtgct atagactctt gttttccttc aaacttggga
     4741 caaatgcagt gtattctgca gatttgtttc gaattgtttt atttatgatg atatacttgc
     4801 cgcacaattc cctgttaagt tagagccttg cagtgcaaaa ctcaagaata aaggcccgtt
     4861 gattgtactc aagtttgaaa atattcttca taatatgaaa ctttcttttc cttgctgaag
     4921 aaatacattg actgattagt aatttcaggt taaatttagt gtttaacttt tttagtaaac
     4981 catcctattg atggatagta gaaacatgac acaaaatcaa ttatcagcga gtattcgcag
     5041 taaaaaagga aagtcggcca atcaaaagtt acgtcaggca ggtaatatcc cggcggtact
     5101 ctacggtcct cggggaaaca ttttgctgga aatggcagaa gaatcaaccc gtcacctgct
     5161 cgaaaaaatg tctggaatgc acgagcttgt accaatcaat gtaacagatt ctgcaagtgg
     5221 agaaagctgg actgcacagg ttttgctgag ggaaattcag aagcatcctt atcaacacca
     5281 aatcactcat attgactttt gggaactgcc tgaagccaag ccgcaaattg tccggatacc
     5341 aatacatgtg attggtgaat caccaggagt taaaggtggt ggagttttgc agatggttgt
     5401 ccgggagata cctgttagct gtcttcctgc agatattccc tcttttgtgg aagtggattc
     5461 ttccagtttg gaattaggtg attctattag aattcaagat gtaaagttgc ctgaaaaggt
     5521 tactgttgga acagacgaga attttgctgt catttcaatt gttggacgtg caaaagaaga
     5581 ggaagaagag attgtagaag ttgcagaagg agaagaagga gtagaagcag tagagggtga
     5641 agaagcagat agtgaagatt ctgttaagga tgaaaaaaca gaggaagagt gaaatttagc
     5701 aagttctgaa acaggaaatg gcggggatga aactggtggc cggcttgggt aatccgggca
     5761 gggagtacga aggaacgccg cataatgttg ggtttcaggt tgttgattta ctcatggaaa
     5821 agctggaggt ttcaggattc cagcaaaaat ttcacagcca gttttgtaga acatcgattg
     5881 aaggtgaggc ttgcattctg atcaagcctc agaccttcat gaatagagtt ggactggctg
     5941 tagcggaatg tgccggtttt tataaaattc ccgcagagaa cattgtcatt atttcagatg
     6001 atttggattt gccgccgggg atggctcgtt ttcgggacgg tggcggtcat gggggacaca
     6061 atggcctgcg atccgttatc gactcattgg gtaacagtaa attccgcaga gtcagatttg
     6121 gtattggcag acctgctggg aaacgagatg ttgtcggtta tgttttggga caatggtcaa
     6181 aagctgaagc aaagcttgct caaagtgtaa tggacctggt gttggtggaa ctgatcggat
     6241 ttttggcaac ttcgcagttt gagaacactt ctttttctgc tccggaattg gcataaggca
     6301 ctgcaaagca ttttgaacaa aacgcattaa gtttccggaa acaaatggca ttagaagttg
     6361 gaattttagg tttgccaaat gttgggaaat ccactttgtt tcgcgctttg accaaagtgc
     6421 aggtagaagc agcaaactat ccgttttgta caattgatcc gaatataggc atcgtccgga
     6481 taagggatcc tagaatgatc cgtttgactg aacttgtcag tccggaaaag attattcctg
     6541 caactttgca gattgtagac attgcaggac ttgtcaaagg tgcaagtcat ggtgagggtt
     6601 tggggaatca gtttctgagc cacatcagac aggttgacgc agttgcacac gtggtgcggt
     6661 gctttgaaga tgaaaacgta gtacatgtag acggcagact tgatccggtt ggtgatgtac
     6721 aggtaataca gactgagttg atattatctg atttggagca ggtagaacgt aaaatagatc
     6781 gactccaacg ccaggttaaa ggtgaccgtg ctttggcggt agagttgggg atttttgaac
     6841 gactgcggga tcatttggat gatggtttac tggccagcaa aatgggtttg agtaaggatg
     6901 aaaaggaact gatcaatgaa ttaagtttgt tgactctgaa accttatctc tacattgcaa
     6961 acatctcaga agatggactc aataatccgg aagttcgtga gcagcagctg cgggaattag
     7021 ccgatgaaga gggggtggaa gttattccga tttgcgcgca ggtcgaatca gagctctcag
     7081 agatggatga tgatgaatca aagcttttca tggaagagtt gggaattgac agttccggac
     7141 tggatcggct aacgcattcc ggataccgga tgcttgagca gattacttat ttcactgcag
     7201 gaaaacagga agtacgggct tgggagattc gcaaaggttc tactgctcct gaggctgcag
     7261 gtaaaattca tacagatttt gaaaaaggct ttatccgcgc cgaagtcttt cattttgatg
     7321 acatcgatca attggggtcg gaatcatctg tcaaggatgc cggacgctgg cgtcttgaag
     7381 gccgggatta tgtagtgcag gatggagaca tcatgcactt tcgttttaac gtatagcatt
     7441 tgtttatttg caaatgttaa gttgcaaagg taaaaaccct ttgctgtttc cttgttcact
     7501 tgagtggact tattgacatt attcgataat ccgaaaggaa tataaatgac aggatatgaa
     7561 cttgtgttca tcacagatcc atcacacaat aatgatgagt tggcttctgt gctgaaaaat
     7621 tataaaaaga ttctcaccaa atccggaggg aagcttattc atgaatatgt ttggggacgt
     7681 cgtcgtctgg cctacgaaat atcaggcaat gactttggtg tttatcatgc ctggtacttt
     7741 acaggtacag gaaaaacaat tgacgaatta cagcgccaac ttggttattc tgatgatgtt
     7801 ttgcgtcatc aaattataaa gactgatgac attgatacag aggcagcatt tttacaaacc
     7861 ttaattccgc ctaaagagga tgcttcagaa aatgaggaaa ctccgaagga atctgaggga
     7921 attacaggag aatctaccaa agttaaaaaa gaatctccaa aaaaagctga ggcagctaca
     7981 gagaatgaaa agaatgtaac tgaagatgtt aaagaagttg acactgaatc agaagttaaa
     8041 atagaaaaat ctgacaaaga tgccgaagaa gtagaaactg aatctgtaga tgcttgaaat
     8101 actttgccag tcagaatttt tcagtgcaga acctaacttt catgaaaatt atttatgtta
     8161 aaaaaacgaa aaagatcaca tttacgtcgt aaacgtatac ccacaagtat tctgcttcgc
     8221 agtaagcgca aatcctgccc gttcaaggag gccggaatag acagaattga ttataaagat
     8281 gttgagttgt taaaaaatta tgtaacccag ggcggaaaga ttattccaag ccgcatttcc
     8341 ggagtatgtg cggcaaatca gagaaagtta aaaaccgcaa ttcgacgtgc acgcaacctg
     8401 gctcttcttt caccaatgaa aggttatgtg cctcaacaag ttaatgttga acgaaaattt
     8461 tccagttaca gaaattctaa accacctgaa tgaagtgtgg gtaagaatcc ccaaatattc
     8521 caaaaactaa gagattgaac aaaaggaaat accgtgaagt tgatactaac tgaagacgta
     8581 cccaatttag ggtctttagg tgacacaata gatgtgaaat ccggtttcgg tcgtaactac
     8641 ctccttccaa agggaatcgc ccttttggct accggcaggg tctccaaaga attacagcac
     8701 cggatacagc atttaaacaa gttgcgtgaa ggaaaaatcg aactggcaca cgaacaggca
     8761 gaaaaactta aaactgttaa actggaagtt gtccgtaaat ccggtcctgg aggtagattg
     8821 tttggttcag tcaccaacag ggacctgaaa gaattgttga aagaaaaaga atttgatgtt
     8881 gaacgaaggt ccatacaatt gaattcgccc attcgaaaca ttggcctcca tgaatttact
     8941 gtgcgagttc attctgaagt ttcagtaaac atgcaaatca aggtgactgg agattcagac
     9001 gagtctgccg ttaaaacagc tgctgtcgat gaaccgaaag ataaaaatgc tgatgaaaaa
     9061 aatgttgaca gtgaagaact tgaaaaactt gaa
//
LOCUS       DTVD01000097            2278 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20454_length_2278_cov_197.992353, whole genome
            shotgun sequence.
ACCESSION   DTVD01000097 DTVD01000000
VERSION     DTVD01000097.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20454_length_2278_cov_197.992353"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="EYO68_03570"
     CDS             <1..360
                     /locus_tag="EYO68_03570"
                     /inference="COORDINATES: protein motif:HMM:PF06253.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIC06914.1"
                     /translation="NGEDYGELSALSKYQIKDIHQDSLNILRDLGKVFLDGEVLDILK
                     KQGAEVEQLKQLVKFPPELVDEYTAKAPDQFTLHARNPEHSIRIGDNWITYSMVSSMP
                     NVSNLNDVRLVGNFNLA"
     gene            361..1146
                     /locus_tag="EYO68_03575"
     CDS             361..1146
                     /locus_tag="EYO68_03575"
                     /inference="COORDINATES: protein motif:HMM:PF12974.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06915.1"
                     /translation="MAFHCATLPLYDLAETRIQTDALWTGLATSFRKKGIKNVPDTLF
                     RGEKTYQKKLFFGQICGYPLTHEYAGRFKVIATPLYRTPYFTGPEYLSVITVHQQCKW
                     QKLEDARGSRVVINSRSSHSGYNILRLMVTPFTKETTFFSKVSVSGAHRQSLSRIRQK
                     QADLASIDGLTWSMLEMYAPEALKGCRILTLTPPAPAPAFVTEKETSQEMLELFRNAL
                     KNAFDEPKFNLVAKNLFLENTQILPENAYDCITEMERISIEDL"
     gene            1143..2033
                     /locus_tag="EYO68_03580"
     CDS             1143..2033
                     /locus_tag="EYO68_03580"
                     /inference="COORDINATES: protein motif:HMM:PF00487.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIC06916.1"
                     /translation="MISRLKPEWQTILLAVLIYALFGFVTRYYHSLPGWFVFIIGGYV
                     VAWHGSLRHEAVHMHPTTSEQLNELLVLPSLDLYLPFRLYAQTHTKHHINQNLTDPEL
                     DPESFYLSEKRWNALPLVRQWSYWIFHTLAGRLLLYPFWMIPYFWFDEAQRLARGDIR
                     NLQAWTWHLLGITLTMSWVIGVCGIPLWEYLLLFVYPGTSLTLLRSFAEHRSHPDWEG
                     RSAILEAEYLFGILYLYNNFHALHHNNPYVPWYKLPSLFRQEREKLLERNNNYLICGY
                     SELFLKYLISPKEIPYLLNR"
BASE COUNT          648 a          481 c          497 g          652 t
ORIGIN      
        1 aatggagaag actatggcga gctgtcagcg ctgagcaagt accagattaa agacatccat
       61 caggacagcc tgaatatttt acgggatttg ggtaaggtat ttctggatgg ggaagtactt
      121 gatattttga aaaaacaagg agctgaagtc gaacaattga agcagctggt aaagtttcct
      181 ccggaactgg tggatgaata tacagcaaag gctccggatc agtttaccct ccatgcccga
      241 aatccagaac acagcattcg tataggcgat aactggataa catattcaat ggtttccagt
      301 atgcctaacg tctccaatct taacgatgta aggcttgtcg ggaatttcaa tttggcttaa
      361 atggcttttc attgcgcaac actgccgctt tatgatcttg cggaaacaag aattcagacg
      421 gatgctttgt ggactggatt ggctacatcc ttccggaaga aaggtataaa aaatgtacct
      481 gacacattgt ttcgaggaga gaagacttac caaaagaaat tatttttcgg acaaatatgt
      541 ggttatcctc tgacacacga gtatgcggga cgcttcaagg taattgccac accgctatac
      601 cggactccct acttcactgg tccggaatac ctgagtgtga ttacggtaca tcagcaatgc
      661 aaatggcaaa aactggagga cgcacggggc agtcgggtgg taattaactc caggtcttcg
      721 cactccggat ataatatcct gcgtttaatg gtaaccccat ttacaaagga aactacgttt
      781 ttcagcaaag tttcagtcag cggagctcat cgtcaaagtt tatcacgtat ccggcaaaag
      841 caggctgacc ttgcttcaat tgacggattg acctggagta tgctggaaat gtacgcccct
      901 gaagctttga aaggatgcag gattttaacc ttaactcctc cggctcctgc tccagcattt
      961 gtaacagaaa aagaaacatc tcaggaaatg cttgaacttt tccggaacgc tttgaagaat
     1021 gcatttgatg aacctaaatt taatctggtg gcaaaaaatt tattcctgga aaatacacag
     1081 attcttccag agaatgctta tgattgtata actgaaatgg aaagaatttc gattgaggat
     1141 ttatgatttc acgccttaag cctgaatggc aaaccatttt gttggctgtt ttgatttacg
     1201 cgttattcgg ttttgtcacc cggtattatc acagtttgcc gggatggttt gtttttatca
     1261 tcggaggtta cgttgtggcc tggcacggct cattgcggca cgaagcagtt cacatgcatc
     1321 ctactacttc tgaacaactg aatgaattgc ttgtgctgcc cagtctggat ttgtatctgc
     1381 cttttcgact atacgcgcaa acccatacta agcatcacat caaccaaaat ttaaccgatc
     1441 cagaacttga tccggaatca ttctatttgt ctgaaaagcg atggaatgca ctgccccttg
     1501 taaggcagtg gagttactgg atctttcaca ccctggccgg acggctgctg ctgtatccgt
     1561 tctggatgat tccatatttc tggtttgatg aagcacaacg ccttgctcga ggtgacatta
     1621 gaaatctgca agcatggacc tggcatctcc tgggaatcac gctcacaatg agctgggtta
     1681 taggagtttg tggtatccca ttgtgggaat atcttttgtt gttcgtatat cccggaactt
     1741 cactcacact gctgcgctct tttgcggaac acaggagtca tcctgattgg gaaggacgca
     1801 gcgctattct ggaagcggaa tacttatttg gtattttgta cctttacaat aattttcacg
     1861 cactgcatca taacaatccg tatgttcctt ggtacaaact tccctcgctt tttcgtcagg
     1921 aacgagaaaa attgctggaa agaaataaca actatttgat ttgcggatat tccgaattat
     1981 ttctgaaata tttgatttcc cccaaagaaa ttccttacct tttgaaccga taaagacctg
     2041 tatttagatc tcaattccat attccagaat ctggaatatt gatatctgat cagctttttt
     2101 ctaatcacaa agtgagtacg aagaaaaatc aatagaataa acgtaaacag gtgtttcaat
     2161 gccaaatgta agtgaataaa ctgaaaactt tagcgatagg taccgctgtg ggctgcccca
     2221 acttttggct tggcaactgc ggcaatcagc ttatcaagcc cgactgaacc aacaggct
//
LOCUS       DTVD01000098            2275 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20490_length_2275_cov_312.815766, whole genome
            shotgun sequence.
ACCESSION   DTVD01000098 DTVD01000000
VERSION     DTVD01000098.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20490_length_2275_cov_312.815766"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            4..1182
                     /locus_tag="EYO68_03585"
     CDS             4..1182
                     /locus_tag="EYO68_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06917.1"
                     /translation="MTSSSVSKPSFDVTCAVPRTGRTLHNFLRKLKSLDANNEEIDQI
                     LKVLAESKRRSTPDLQEALSFLQEISGKAPHCFSISVDRKRKQRPYRLGFLAYEWQIG
                     KTKIRVEGEEPHNGSSLIKLDEVDFTVVGLDELLSMTQHYLGDPTNVTKWGMYNYQLD
                     KPTSIRVAGSAMLTSYNDLLGGEVQDMVGFFLISKKGGSSRSPIDFETLSRHGRYVFV
                     KGRYSGIVMAAYPQLQVEPVEDVEEAVMNGEKGSVGLEIVQSGNTLKSKGLLLHGSPL
                     FLSESLYVVDYDRFQQNKALRKLVETLNPVGYFEDVRLENFSRWYYALEQNLGDSWVQ
                     RPPVDELFCKTDDIDSGLRPYRLRTRNWKPDDRYLREEAIELANSSRQKVLKHYKELH
                     "
     gene            complement(1309..1983)
                     /locus_tag="EYO68_03590"
     CDS             complement(1309..1983)
                     /locus_tag="EYO68_03590"
                     /inference="COORDINATES: protein motif:HMM:PRK09347.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HIC06918.1"
                     /translation="MDLLKLCFYYNSNLVYGILKINKSKRIFMRRNKVDKDKVQQSIQ
                     LLLEGLGVDLDNTHYRKTAERAANAWVEELCSGLGEKKFTLTTFPIGNNYEPSMVVLQ
                     HIPVRSVCAHHLLPFYGEATVAYIPDERLCGLSKLSRIVDYFARRPQVQEELTSDITN
                     FLCEQLSPQGAGVIIKATHMCMAMRGVSHDGVMTTSSLKGSFLNDGTVRAEFMALANT
                     QSLNKL"
BASE COUNT          676 a          458 c          506 g          635 t
ORIGIN      
        1 attttgacct catcatccgt cagcaaacct tcatttgacg ttacttgtgc ggtaccacgc
       61 accgggcgca cattacataa ttttctgcgt aaactgaaaa gcttggacgc aaacaatgaa
      121 gagattgatc aaattttgaa agtcttagct gaaagcaagc gcaggagtac acctgatctg
      181 caggaagcac tgagtttttt acaggaaatt tccggaaaag cgccgcactg tttttcaatt
      241 tcggtggatc gaaagcgtaa acaaagacct tatcgtttgg gattccttgc ttatgagtgg
      301 caaattggaa aaacaaaaat ccgtgtggaa ggagaggagc cgcataatgg ttcttcactg
      361 atcaaactgg atgaggtgga tttcacagtt gttggactcg atgaactgct ttcaatgacc
      421 cagcattacc ttggtgatcc tacaaatgtg acaaaatggg gcatgtacaa ttaccagttg
      481 gacaagccga caagtattcg tgtagcaggt tctgcgatgc tcaccagcta caatgatttg
      541 cttggaggag aagtacagga tatggtggga tttttcctga tttcaaaaaa aggcggcagc
      601 agccggagcc caattgattt tgagacgctt tcgaggcacg gcaggtatgt gtttgtcaaa
      661 ggccgttata gtggaatcgt gatggccgca tatccgcagc tacaggtaga accggtggaa
      721 gatgtggaag aagcagtgat gaatggtgaa aaaggtagcg ttgggcttga aatagtgcag
      781 tcagggaaca cgcttaaatc aaaaggcctg ctgctgcacg gctcaccctt gtttttatcc
      841 gaaagtcttt acgtggttga ttatgaccgt tttcagcaaa ataaagcgct cagaaagctt
      901 gtggaaacac taaatccagt cggatatttt gaggatgtgc ggctggaaaa tttttcccgc
      961 tggtactatg cacttgaaca aaatctgggt gattcttggg ttcagcgacc tccggttgat
     1021 gaactgttct gtaaaacaga tgatattgat tccggtttgc gtccttaccg cctgcgaacc
     1081 aggaactgga aacccgatga ccgttacctg cgtgaagaag ccattgaact ggcaaatagt
     1141 tcacggcaaa aagtcctgaa acattataag gaattacact aacttgatgg tcttgtaata
     1201 agcctaaaat gggactaaat cagaattctc cacagagtca aagataatac aaaggttcca
     1261 ttttaataat tttttaactg agacccctga agctctgtgg gtagattttt acaatttatt
     1321 caaactctga gtatttgcca aagccataaa ttcagcccgc acggttccat cgttgaggaa
     1381 tgaacctttg agagaactag tagtcataac accatcatga ctgaccccgc gcattgccat
     1441 gcacatatgc gttgctttaa ttatcactcc ggcgccttga ggagagagct gttcacacaa
     1501 aaagttggta atatcgctgg ttaattcttc ctgtacctgc ggacgtctcg caaaataatc
     1561 tacaattctg gaaagctttg aaagtccgca aagtctttca tccggaatgt aggctacagt
     1621 cgcttcacca taaaaaggca gcaaatgatg agcacaaact gaccttacag gaatatgctg
     1681 cagtaccacc atgcttggtt cataattgtt accaatagga aaagtggtca gggtaaattt
     1741 cttttcacca agtccggaac agagttcttc gacccaggca tttgccgccc gttccgcggt
     1801 ttttctgtaa tgcgtattgt ccaaatccac tcccaaacct tcgagcagaa gttgaatact
     1861 ttgttgtact ttatctttat ccactttgtt cctcctcatg aaaattctct ttgacttatt
     1921 aattttaaga ataccataca ctagattaga attgtagtaa aaacagagtt tcagcaagtc
     1981 aatctacaga gaagaaatcc agatagaaca acagattctg ttctggctgt aaaacttgta
     2041 tgagctattg ttctgcaaca ggtatccagg aaatttacac gattaaatgt aaattcagat
     2101 caagaaaaac ttgccaaatg ttcagtaatt atttacaaat aaagggatct gttttagatg
     2161 agaaacttca ttcagtccag gtatttgcag gatagtttat ttttggcaac ttcctcaaag
     2221 ctcttcggag gggtgggtga gtggcttaaa ccagtacatt gctaatgtgc cgtac
//
LOCUS       DTVD01000099            2273 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20510_length_2273_cov_443.180343, whole genome
            shotgun sequence.
ACCESSION   DTVD01000099 DTVD01000000
VERSION     DTVD01000099.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20510_length_2273_cov_443.180343"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="EYO68_03595"
     CDS             <1..158
                     /locus_tag="EYO68_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine sulfoxide reductase"
                     /protein_id="HIC06919.1"
                     /translation="KKADSHLGHLFNDGPKPDGLRYCINSAALRFVSKNDLEKEGYLI
                     YSSVFQD"
     gene            491..>2273
                     /locus_tag="EYO68_03600"
     CDS             491..>2273
                     /locus_tag="EYO68_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIC06920.1"
                     /translation="MGIATDLILLVVTAFFSGLLMQRLGQPLILGYILTGIAFGPYTG
                     GFALTSVHEIELLAEIGVALLLFALGLEFSLKDLKPVKKIALIGTPIQIILTIGMGYG
                     IGQLMGWDAKSSLWLGALVSLSSTMVLLKTLMNQGWLGTLSSKVMVGMLIVQDLAVVP
                     LIVLLPMLNDPSLGWISLEIAILKTVVFLAGMILFGTRLLPLIMRYIAHLGSRELFLL
                     AITAIGLGVGYLTYLVGLSFAFGAFVAGMVLSESDYGHQALSDIIPVRDLFGLLFFAS
                     VGMLLNPGFLLDHWKQVLMLVLIVSLGKGIIFALLARIFKYGNVVPLAVGLGLFQVGE
                     FSFVLAQVGVSTNSISHEVYSLVLTTAIITMFLTPIISGQTAHLYALSKRWFQHEPLD
                     TINYPKSGLRNHVIIVGGGRVGFRIAQVLKRLSVPLLIIELDQLRVEQAKNSEITVIY
                     GDASHEVVLEAAETSSARMLIITSPEVVIAQAIVENARKVNTEIQVVARAPGVEFLEE
                     FKKLNISEVVIPEFEAGLEMARKALVHLHIPAAKIQHYTESLRQDLFASLFSENEEDK
                     ILEQLQWAEHQFDLQWMLIKPGSVLVGK"
BASE COUNT          552 a          463 c          558 g          700 t
ORIGIN      
        1 gcaagaaagc agattctcat ctgggacact tgtttaatga cggcccaaaa ccggatgggt
       61 tacgttattg tatcaattct gccgcacttc ggtttgtttc aaaaaatgat ttagagaaag
      121 aaggctatct tatttatagc agcgtatttc aagactgatc attgacagat tttttttgag
      181 tttgtcggct ctttccaaat tattttatct actatcctga agttactggg taaaaggttg
      241 gcagaacaga tctatttctc tgtgttgatg tgacctccat ctgtcgccat tgtacatcca
      301 gccgactaga cttcacgcac gatggaatcg ttgcagataa taaaccagcg atccaggaaa
      361 tcatcaaagt cacccccact actgtcaatg attttgtatt gacaaattat ttgtatgggc
      421 ataaataaaa gcaggaaaaa acttaacctg taaaaacctc cagaaaatat ttctcattca
      481 tttttactct atgggcattg ctacagatct cattctgctt gttgttacag cgtttttcag
      541 cggcctcctt atgcagagac tgggacagcc gttgattttg ggatacattc taaccggaat
      601 tgcgtttggt ccatacaccg gcggattcgc gctgaccagc gtacacgaaa ttgaactact
      661 ggcggaaatc ggagttgcgc ttttgctgtt cgcacttggt ttggagtttt ctctcaaaga
      721 cctcaaacct gttaaaaaga tcgcgctgat cggaacgccg attcaaatta tactgactat
      781 tggaatgggt tacggcattg ggcagttgat gggttgggat gctaagtcct ccctttggct
      841 aggcgcgctg gtttctctgt caagcacaat ggtcctgcta aaaactttga tgaatcaagg
      901 ctggctggga acgctttcca gcaaggtcat ggtgggaatg ttgatcgtac aggatttggc
      961 ggttgttccc cttatcgtgt tgctcccgat gttgaacgat ccctctctgg gttggatttc
     1021 cttggaaatc gctattttga aaacggttgt atttttggca ggaatgattt tgttcggaac
     1081 acgtttgctg cccttaatta tgcgctacat tgcccatttg ggttcacgcg aattattttt
     1141 gcttgccatc actgcaattg gtttgggcgt gggatatctt acgtatctgg tggggctttc
     1201 ctttgctttt ggagcatttg tcgccggaat ggttttgagt gaatcagatt atggacatca
     1261 ggcgttgagt gacatcatcc cggttcggga tctttttggt ttattatttt tcgcatccgt
     1321 ggggatgctg ctcaatcccg gatttttgct ggatcactgg aaacaggttc tcatgcttgt
     1381 tttgatagtc agtctcggta aaggcattat tttcgcgttg ctggcccgga ttttcaaata
     1441 cgggaatgtg gtgcccttag ccgttggttt ggggctcttt caggtgggag aattttcgtt
     1501 tgtgctggca caggttgggg tctccacaaa ctcaatcagc catgaagtct attcactggt
     1561 tttgaccaca gcgataataa cgatgtttct aacaccgatt atttccgggc aaaccgcgca
     1621 tctgtatgca ctcagtaaac gctggttcca gcatgagccg ttggacacca tcaattatcc
     1681 taaatccgga ttacgaaatc atgtgattat tgtaggcggc ggacgtgttg gtttccgaat
     1741 tgcccaggtt ctgaaacgcc tcagcgttcc cctgttgatt attgaattgg atcaacttcg
     1801 ggtcgagcag gcaaaaaatt ctgaaattac agttatttat ggagacgcaa gtcacgaagt
     1861 ggtattagaa gcggccgaga cttcttcggc gcgcatgtta atcattactt ctccggaagt
     1921 agtgattgcg caggcgattg tcgaaaatgc gcgaaaagtg aatactgaaa ttcaggtggt
     1981 ggcacgtgca ccgggtgttg agtttttgga agaattcaaa aagctcaata tttctgaggt
     2041 ggtgattccg gaatttgagg cgggacttga aatggcgcgc aaggccctgg tacatttgca
     2101 tattccagcc gccaaaatcc agcattacac tgaatccttg cggcaggatt tgtttgcctc
     2161 tcttttcagt gaaaatgagg aagataaaat cctggagcag ctccagtggg cagaacatca
     2221 gtttgacttg cagtggatgt tgataaaacc cggaagcgta ctggttggta aaa
//
LOCUS       DTVD01000100            2259 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20720_length_2259_cov_370.387931, whole genome
            shotgun sequence.
ACCESSION   DTVD01000100 DTVD01000000
VERSION     DTVD01000100.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_2259_cov_370.387931"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(2..1894)
                     /gene="uvrC"
                     /locus_tag="EYO68_03605"
     CDS             complement(2..1894)
                     /gene="uvrC"
                     /locus_tag="EYO68_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIC06921.1"
                     /translation="MNKETRHAVKGGGDISSARDKLSNLPNLPGVYLFKDDQQSILYI
                     GKSKSIRNRVRSYFNNSAKHNLRIQLMVSRIHDFSLIVTDTEAEALILEEQLIKRHHP
                     RYNVALKDGKSYPYCKLTVGEMYPRLMLVREKIDAKSEYYGPYPSVKDARQVLKAVMT
                     YFPLRTSKMLLDGKKTYRPCLNFQMKRCLAPCRGVVSVEEYGKVVRQVRLFLKGRDKE
                     LLRELEQRMEQSSKKLEYEKSAQYRDQIRAMSRIFERQMVLDVKGKDQDVFNLFREAD
                     STGVQVLFIRNGRLLGTDFFFFEDSSEASADNLLGQALHRIYMPEGSIVPREILLPFE
                     YTDRKLLEDALNKKSERRVYVVCPQKGRKKELVTMAYNNAKVNLSEERRRYAKNTNIL
                     KHVKYELKLNRLPEVVEAFDISHLSGTMTVASMVCWKNNSASNKDYRKYKIKSISGPD
                     DFASMKEVLTRRYKRTLAEGEKLPDLILIDGGKGQINIAEKVLAELGILRKVDLIGLA
                     KGRSARKMGRSRGQNVDYEYVVKPKQKNEIRMRRNSDVLYFLQNIRDESHRFAIEFQR
                     KLKRKDTLHSRIDDLQGIGPKRRRLLLKHFGSLTGLRQASLDEIMQVPGIPQNLAQEI
                     QDFLQS"
     gene            complement(1900..>2259)
                     /locus_tag="EYO68_03610"
     CDS             complement(1900..>2259)
                     /locus_tag="EYO68_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HIC06922.1"
                     /translation="GQDDSPLRLYYVARKIGKADLIKDELFKASFKHGVNVFDPGIIN
                     YLARSLGMHKEFQKEKDQKWVNQLIKDGERKAALYGVSGTPTVVIQYALKMDIGPYGT
                     MADFVKKVPETIADLMQ"
BASE COUNT          570 a          529 c          460 g          700 t
ORIGIN      
        1 ttcagctttg cagaaaatcc tgtatttcct gagccaggtt ttgcggaatc cccggcacct
       61 gcattatttc gtctaaagac gcctgcctta aaccagttaa actgccgaag tgcttcagta
      121 aaagtcgccg acgctttggt ccgattccct ggagatcatc aattcgcgaa tgcagtgtgt
      181 cttttcgttt gagtttgcgc tgaaattcaa tggcaaaacg gtgcgattca tctcggatgt
      241 tctgcaaaaa ataaagcaca tcggagttac ggcgcatgcg gatttcgttt ttctgctttg
      301 gtttcactac atattcataa tcaacatttt ggccccggct gcgtcccatc ttcctggcgg
      361 accgtccttt tgccagtcct atcaaatcga ctttccgtaa aataccaagt tctgccaaaa
      421 ctttttcagc aatattgatt tgtccttttc cgccgtcaat taaaatcaag tccggaagtt
      481 tttccccttc cgccagagtg cgtttgtaac gacgcgtgag aacttctttc atactcgcaa
      541 aatcatccgg accggaaatg ctcttgattt tgtatttccg gtaatcttta tttgaagcag
      601 agttattctt ccagcagacc attgaagcaa ctgtcattgt tccagaaaga tgcgaaatat
      661 caaaggcttc cactacttcc ggaagccgat tcaattttaa ttcgtactta acatgcttca
      721 aaatattagt gttttttgca taacgtctgc gctcctctga taaattaact tttgcattgt
      781 tgtatgccat tgtgacaagt tcttttttcc gtcctttctg aggacaaaca acgtaaacgc
      841 gccgttccga ttttttattg agtgcatctt caagcagttt tctatctgta tactcaaagg
      901 gcaataatat ttcacgggga acaattgaac cttccggcat ataaattcta tgcagtgcct
      961 gtcccagtaa attatctgcg gatgcttcgc tgctgtcctc aaaaaagaaa aaatctgttc
     1021 ccagcagtct gccgttgcga atgaacaaaa cctggactcc ggttgagtca gcttcacgga
     1081 aaagattaaa cacatcctga tccttgccct tgacatcaag taccatttgt ctctcgaaaa
     1141 tccggctcat ggctcgaatc tgatcacggt actgagctga tttttcgtac tcaagttttt
     1201 tggacgactg ctccatccgc tgttccagtt ccctcaggag ttccttatca cgacctttta
     1261 aaaacaagcg aacctggcgc accacttttc catactcttc cacagagaca acaccccgac
     1321 atggagcaag gcagcgcttc atttgaaaat tgagacaggg acggtacgtc ttttttccat
     1381 caagcagcat tttgctggta cgcagcggaa aatatgtcat gactgctttc aacacctggc
     1441 gggcatcttt cacagaagga tatggtccat aatattctga ttttgcatca attttttcac
     1501 gcaccagcat aaggcgcgga tacatttctc caacagtcag tttgcagtac ggatacgatt
     1561 ttccatcctt gagagccacg ttgtaacggg gatggtgtct tttgatcaac tgttcttcca
     1621 gaatcagcgc ttctgcttca gtgtctgtaa caatcaggga aaaatcatgg atacgggaga
     1681 ccatgagttg gatccgtaaa ttgtgtttgg cagagttgtt aaaataggaa cggacgcggt
     1741 tgcggatgga tttggatttg ccgatgtaga ggatgctttg ctgatcatcc ttgaaaagat
     1801 aaactccggg aaggttcggc aggttggaga gtttatcccg tgcggaagaa atgtctccgc
     1861 caccttttac agcatggcgg gtttctttgt tcattttgat tactgcatca aatctgcaat
     1921 tgtttcagga acttttttta caaaatcggc cattgttcca tatggtccaa tgtccatttt
     1981 cagcgcatat tgaatgacca ctgttggtgt gccggacaca ccataaaggg ctgcttttcg
     2041 ttctccgtcc ttgatcaact ggttgaccca cttttggtct ttttcttttt gaaactcttt
     2101 gtgcattcca agcgaacgtg ccagataatt tataattccg ggatcaaaca catttacgcc
     2161 atgcttgaaa cttgccttga aaagttcatc cttgatcaaa tcagctttgc caatttttct
     2221 agccacatag tagagacgca aaggggaatc atcctgtcc
//
LOCUS       DTVD01000101            2250 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20859_length_2250_cov_314.106606, whole genome
            shotgun sequence.
ACCESSION   DTVD01000101 DTVD01000000
VERSION     DTVD01000101.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20859_length_2250_cov_314.106606"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            178..2046
                     /locus_tag="EYO68_03615"
     CDS             178..2046
                     /locus_tag="EYO68_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC06923.1"
                     /translation="MFEWFESRLNPYPPEKPGTPPNGILAFCWHYSGSAWPWLALMSL
                     LTASISISEVYLFGFLGNIIDWLVVSDRVGFIEREGDRLFWMGSFIIVGLPCIVLFQS
                     LIIHQTLLGNFPMIARWQMHRYLLFQSMSFFTNEFAGRVATKVMQTSLAIRESVMKML
                     DVFVYVTVYFLSMMVLIANSELRMILPLLLWMLVYVSLIIFFVPKLKVISMEQADSRS
                     LMTGRVVDSYTNISTVKLFSHALREEIYAKEGMDSFLGTVHRQMRLVTLFHFFVYFNN
                     VLLVFGISGLAIWLWIYSSVSVGSIAVAIAMCLRINGMSQWIMWEVSSLFENIGVVQD
                     GMKMMAKPQEVVNLSDAREIIVEEGRVEFENISFHYGRTDGIIDNFTLSIVGGEKVGI
                     VGRSGSGKSTLLNLILRFYDLESGRILIDGQDISRVLQESLRSNIGVVTQDTSLLHRS
                     IYDNIAYSSPETSKEKVFEAAKKANAWEFILEQEDSQGRRGMEAHVGERGVNLSGGQR
                     QRIAIARMFLKDAPILILDEATSALDSEIEAVIQENLFKLMEGKTVIAIAHRLSTIAQ
                     LDRLVVIEKGNVVESGTHEKLVNNSLIYSDLWKRQSGGFLAPEEYEKLHSNSINTL"
BASE COUNT          649 a          432 c          478 g          691 t
ORIGIN      
        1 accagatatg ccgccttttt tcaactcatc aaacaccaga ttcgattata actcagctgc
       61 caatcagagt aacatttaag gctaggaaac caatattgcc ttagagggcg ttttaaccac
      121 ttttttcaaa ttagcataag ctaaactgat tacactaaaa taaaatgaca tttaaaaatg
      181 tttgaatggt tcgaatcaag actgaatcct tatcctccgg aaaaacctgg tacgcctcca
      241 aatggtattt tagctttctg ctggcattat agcggttccg catggccatg gttggcactt
      301 atgtctttac tcactgcttc catttccata agtgaagtct acctcttcgg ttttctgggc
      361 aatattattg actggttagt ggtaagtgat cgtgtgggtt tcattgaacg tgaaggagat
      421 agacttttct ggatgggaag cttcattatt gtgggtctgc catgcattgt actgtttcaa
      481 tctcttataa tccatcagac tcttctagga aacttcccaa tgatcgcccg ctggcagatg
      541 catcgatatc ttctttttca gagcatgagt ttctttacaa atgagtttgc tggaagagtg
      601 gctaccaagg tgatgcagac atcattggcc attcgagaaa gtgtgatgaa aatgcttgat
      661 gtcttcgtat acgtcactgt ctatttcctg tccatgatgg ttctaattgc aaattcagaa
      721 ttgcgtatga tacttcctct tctcttgtgg atgttagtct acgtttcttt gataattttc
      781 tttgttccaa aactgaaagt tatatccatg gaacaggctg attcacgatc tttaatgaca
      841 ggacgagtgg tagatagtta caccaacatt tccactgtaa aactgttttc ccatgcactt
      901 agagaagaaa tttatgccaa agaaggaatg gattcttttc taggaacagt tcatagacag
      961 atgcgccttg taacattatt ccattttttt gtttacttca ataatgtttt actagtattt
     1021 ggtatatctg gacttgcaat ctggctttgg atttattctt ctgtttcagt tggttcgatt
     1081 gccgtagcaa ttgccatgtg tttacgtatt aatggcatgt ctcaatggat tatgtgggaa
     1141 gtttcaagtt tgtttgaaaa tataggtgtt gtgcaagatg gcatgaaaat gatggccaaa
     1201 ccacaagaag tggttaacct ctctgacgct agagaaatta tagttgaaga gggtcgagta
     1261 gagttcgaaa acatcagttt ccattacggt cgtactgatg gaattataga caactttacc
     1321 ttgagtattg ttggtgggga aaaagttgga atagtcggac gatctggctc tggcaagagt
     1381 acactcctga atttgatact acgtttttat gacctagaat ctggccgaat cctcattgac
     1441 ggacaggaca tctcaagagt ccttcaggaa agtttgcgat ctaacattgg tgttgtaacc
     1501 caggatacct cattgctcca ccgatcaatt tacgacaata tcgcatattc ttctcctgag
     1561 acgagtaaag aaaaggtgtt tgaggcagct aaaaaagcca atgcttggga attcattctg
     1621 gaacaggaag attctcaagg gcgccgtggt atggaggcac atgtaggtga acgtggcgtt
     1681 aacctttcag gaggccaacg tcagcgcatt gctattgcaa gaatgttcct taaggatgcc
     1741 cctatcctaa ttcttgatga agccacatca gctctggatt cagaaataga agccgtgatt
     1801 caggaaaatt tgttcaaact gatggaagga aaaacagtga ttgcaattgc ccaccgactt
     1861 tccactattg cacagttgga tcgtcttgta gttatcgaaa aagggaatgt ggttgaatct
     1921 ggaactcatg aaaaattggt caataattcc ctgatctatt ccgacctttg gaagcgacaa
     1981 tctggagggt ttctagcacc ggaagaatat gaaaaactgc attccaattc aataaataca
     2041 ctttaagtgt attcatcaat taaagagtaa aaacattcaa ctacccatat aatccaaagt
     2101 gagcggctca ttgtatcaaa taccaaaata tgcttaaaac gctccctcac cttgcttggc
     2161 aaccactgat actgactgag cagaggccct ggcccgaaag aatcgatgaa ggggggtggg
     2221 gccacaaaac gggcatcaag ctttatatat
//
LOCUS       DTVD01000102            2245 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_20942_length_2245_cov_293.482648, whole genome
            shotgun sequence.
ACCESSION   DTVD01000102 DTVD01000000
VERSION     DTVD01000102.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20942_length_2245_cov_293.482648"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            20..2239
                     /locus_tag="EYO68_03620"
     CDS             20..2239
                     /locus_tag="EYO68_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase large subunit"
                     /protein_id="HIC06924.1"
                     /translation="MIPLIGYVNKLSARPGDTLEFKVSSTFNVPFQASLVRIRCSDPN
                     PAGPGIREKSVDASFTGQYPSRIQNIHLGSYGIVDSARPLEDLNSFTMSTTIWPTTPK
                     KGRQAIIAWGDQKRSGGVLGIKKDGLLFGEIGKTNINHSKPLNSRHWYTVWMAYDAAE
                     QTLSIGCLPLSAKHTEPTTIIETINLDMPPDRMSSPLLFGAEQRDCICSHFNGKIERP
                     RIHNFALGVENFPIARNERCFADWDFAKEMSSTRLIDNGSHELNGRLVNLPARAMTGS
                     NWTAKEMCWRHAPEQYGAIHFHDDDLHDCDWQTDFIFLVPDDLASGLYGAKLKCRDRE
                     DIIPFVICPPKGKNTADLCLVIPTFTYVIYANHARPEFGDPWRKRAKDWDAYPWNPGD
                     HPEYGLSTYNFHTDGSGICHSSALRPILTMRPGYFTIVDHNGSGLRHLQADTHLIDWL
                     EEQEFSYDLISDQEVHEEGRLVLEPYRTVLTATHPEYHTNKTLDAIEAYRDGGGRFVY
                     LGGNGFYWKVAMHLEEKSVIEIRRGEGGIRAWAAEPGEYYNAFDGEYGGLWRRNGRPP
                     QQIAGVGFSAQGTFFGSYYQRLEASFESRFSWAFKGIDDKRLGDFGLSGGGAAGYELD
                     RADVRLGTPENAVVLASSEGHSRDYTGDEEDFFVLVPEEQLTHMGTWPGDSIKNLIRA
                     DMVFYQTANGGAVFSVGSITFCGSLPCNDYNNNISKLLRNVIERFLDPNPDFGKEVY"
BASE COUNT          605 a          487 c          535 g          618 t
ORIGIN      
        1 aacttcaaaa atttttaaaa tgatccctct aatcggttat gtgaataagt tatctgcacg
       61 tcccggtgat acattggaat tcaaagtcag ctcaacattt aacgttccat ttcaagcaag
      121 ccttgttcgc attcgctgct ctgatcccaa cccggcagga ccaggcattc gtgagaaaag
      181 cgttgatgca tctttcactg gccaatatcc atcacgcatc cagaatatcc accttggttc
      241 ttatgggatt gttgattctg cccgacccct tgaagacttg aacagcttta caatgtctac
      301 cacaatttgg cctacaacgc caaagaaagg gcgtcaggca attatagcct ggggtgatca
      361 gaaaagaagt ggtggtgtac tcggaataaa aaaggacggg ctactgtttg gtgagatcgg
      421 aaaaactaat atcaaccact caaaacctct aaatagcagg cactggtata ctgtctggat
      481 ggcatatgat gctgcagagc aaactttgtc tataggctgt ctgcctcttt cagccaaaca
      541 cacagagccg actaccataa tcgaaacaat aaatctcgat atgccaccag ataggatgtc
      601 ttctccgctg ctcttcggcg cagaacaaag agattgtata tgttcccatt tcaatggcaa
      661 gatagaacgt cccagaattc ataactttgc tctaggcgtg gagaattttc ctattgccag
      721 aaatgagaga tgttttgccg actgggattt tgcgaaagaa atgtcgtcga cgagactgat
      781 tgataacggc tctcacgaac tcaacggacg actagtcaat ttgccagccc gtgcaatgac
      841 cgggtcaaat tggacagcga aagagatgtg ttggcggcat gcacctgagc agtatggggc
      901 catccacttt catgatgatg acttacacga ttgtgactgg caaactgatt ttatctttct
      961 tgttccggat gatctcgcct ccggattata tggtgctaag ttaaagtgta gggatcgaga
     1021 agatatcatt ccttttgtga tttgtccgcc aaagggcaag aacacagcgg atctgtgtct
     1081 tgtcattcct acattcacct acgttattta tgcaaaccac gcgagacctg aatttggtga
     1141 tccttggaga aaaagggcaa aggactggga tgcctatcca tggaaccccg gtgatcaccc
     1201 agaatacgga ctctcaacct ataatttcca cactgatgga agcggaatct gtcactcctc
     1261 tgccctccgc ccaattttga caatgcggcc tggttatttc actattgtgg atcataatgg
     1321 ctctggcctt aggcatttac aggccgacac tcatctaatt gattggttgg aggaacagga
     1381 attctcttat gaccttatat cggatcaaga ggtgcatgaa gaaggtcggc ttgttctcga
     1441 accctatcgc actgtgctga cagcaactca cccagaatat cacacaaata agactctgga
     1501 tgcaattgag gcataccgag acgggggtgg ccggtttgta tatttgggag gcaacggttt
     1561 ttactggaaa gtggcaatgc atctagaaga aaagagtgtc atcgaaattc gtcgaggaga
     1621 aggtggtatc cgtgcctggg cagctgaacc gggagaatat tataatgcat ttgacggtga
     1681 atatggtggg ctttggcgac gtaatggtcg tccgcctcag caaatcgctg gagtcggttt
     1741 ttcagcgcag ggaacttttt tcggatccta ttaccagcgc ctagaggctt cctttgaatc
     1801 gcgattttct tgggctttta aaggcattga cgataagaga cttggtgatt ttggactttc
     1861 tggcggtggc gctgcaggat acgaattgga ccgtgcagat gttcgattag gcactccaga
     1921 aaatgcagtc gtcttagcaa gttcagaagg ccacagtcgt gattatactg gggatgaaga
     1981 ggactttttc gttctagtac cagaagaaca attgacgcat atgggtactt ggccaggtga
     2041 ttccatcaaa aacctaatcc gcgctgatat ggttttttac caaactgcta acggtggtgc
     2101 ggtcttttcc gtcggttcaa tcacgttctg tgggagcttg ccttgcaatg attacaacaa
     2161 caatatttca aagttgcttc gcaatgtaat tgaacgtttt cttgatccta atcctgattt
     2221 tggaaaagag gtatattaat tcttg
//
LOCUS       DTVD01000103           28836 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_210_length_28836_cov_432.218234, whole genome
            shotgun sequence.
ACCESSION   DTVD01000103 DTVD01000000
VERSION     DTVD01000103.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 28836)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28836)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28836
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_28836_cov_432.218234"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="EYO68_03625"
     CDS             <1..210
                     /locus_tag="EYO68_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06925.1"
                     /translation="ESEEDILRTLKLEPRHFGALSGQGLIRMALEDWSGAITVLEAGL
                     RIHPHMPGVIRNLEYVRKKQKESMT"
     gene            complement(306..530)
                     /locus_tag="EYO68_03630"
     CDS             complement(306..530)
                     /locus_tag="EYO68_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06926.1"
                     /translation="MTQTEEKIENFANSVGVDLSHQFEKQVSRTIKELMDSLESHLID
                     DNTKVDYATHVFAKALDDMVDYLETPKLLN"
     gene            740..1162
                     /locus_tag="EYO68_03635"
     CDS             740..1162
                     /locus_tag="EYO68_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06927.1"
                     /translation="MYSQLSHFKERIDETFEIIFSFRKPAAVLIFLWIGISSVEAQEY
                     ATDRLFMKEYSKAKCRNEVENKIRRLKNNVDMTLEHQAFLNRNIWSKLHTNLPLSRGE
                     KKHLNDLKQKGIPLKKIRSKDYWAYNAAQFRALRLKCK"
     gene            1632..4109
                     /locus_tag="EYO68_03640"
     CDS             1632..4109
                     /locus_tag="EYO68_03640"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIC06928.1"
                     /translation="MALQTPLSWLQDFVELNQSPDDLAERLTTAGLEVETIEKIGENW
                     GEFCVVGQIKKIRKHPNADALHLVDVEFGADKPITLVTGAPNIREMGNSIPEPAPKVA
                     LALTGAMLIDAYHEDRALKKLKPAKIRGIASEGMLCSELELGLSEEHEGILILPDDAP
                     VGTLLQDFLGDTILHFDIKGGFSHLLSMLGVAREIAALTGQKLDKSVLPDLSRTEVLE
                     QPPFVELEIKDPDICPRYTALLIRNVKIGPSPFWMQQRLLRIGMRPINNIVDITNYVM
                     LELGQPLHAFDYDKLIERANGDTPKIIVRRAKKGETLLTLDYVERKLDSEMLMITDSA
                     GLIAIAGVMGGGDSEVTETTKNVLLESANFEFINNRRTSQVLKLRTEASERFGKQIDP
                     EGTLQAALRAALLMVENGSGTLEKIAGDLYPRPKETVSLELDPFYVERLLGIKISADQ
                     ISEILNSLEFKVSGKNILKITVPSHRMDIRIPADLVEEIIRIYGYENLPSTLLDDELP
                     PQHSNQKLEGTERVRDILVASGMDEIITYSMMDPMDEARLRLENDVDFAKFVPLKNPL
                     TQERSHLRRSLLPGALHTAHANLRFLDKVVMFEVGSVFHPQKGKKLPEEPQRLSLLMC
                     GLRNSQSWLEETEGTYDFFDLKGVLEHFLSALHIEGIEWKKSRELPCHPGRCVQILVN
                     GKELGFSGELHPKIRSAFELPEQAVCIAELDLDLIIKLGIENHQMDFISNFTPIFEDL
                     AFVMDSSLPVEAITPVILQTGKPLLRKATLFDVYEGEQVDEGKRSLAYSLTFQANDRT
                     LTDEEVGKIRNKIVKRLQHEFRATLRA"
     gene            4118..4447
                     /locus_tag="EYO68_03645"
     CDS             4118..4447
                     /locus_tag="EYO68_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIC06929.1"
                     /translation="MMKTLLHLRKAFGHSMKGLRETFRNEMAFRIELTAAVILIPTAL
                     ILSVSPIVRIMLVGSVFLVLIVELLNTGIETVVNRISAEQHHLSGMAKDAGSASVFVA
                     SLNLVFV"
     gene            4503..4736
                     /locus_tag="EYO68_03650"
     CDS             4503..4736
                     /locus_tag="EYO68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIC06930.1"
                     /translation="MIFYAVLQETISEDPLLLEVKKDCTAEQVLELLAEKFPQAAPIL
                     KSTRLANEDEYVGKQTLLTDGAEYCLIPPVSGG"
     gene            4776..7016
                     /locus_tag="EYO68_03655"
     CDS             4776..7016
                     /locus_tag="EYO68_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIC06931.1"
                     /translation="MSRIANTKIATGVFWVEVPEAELYVLCGCPADSVKHLMKAGKIR
                     NLDRDVGSLEPGSVSFQHHHGTVTNETGPNAILLSDLNIQNGDFANLAEFPVLQMLYR
                     QGMLLPNHPNNTGAKPFIIGHKNMVNAQMEYIHRGNYGLTSLEEILDAGIPQKQAEEM
                     MRIKLHFAFGAVRPSSDLLEARIVDHEPVEILNGVHIARKSMNCYEFTYKDESSEINL
                     NLSHDERYETPYELKNHHFKRDYFSIAHTGEGDGWDINRPCMASVISYQGKIFLIDAG
                     PNIALTLNAIGVDVNEVEGIFHTHAHDDHFAGLTTLARANHRIKYYSTALVRASVTKK
                     LSPLLSISENEFEKYFEVCDLVFDKWNNIDGLEVRPVFSPHPVETNILYFRTLWENGY
                     ATYAHLADIASHDVLTKMVEVDKKLPGISPKLKKKVWEDYLSPVQVKKIDIGGGIIHG
                     KAKDFLTDKSDKIILAHTAHTLTKDEEKIGCGVTFGSTDILIEGHEDYALQAGGNYLR
                     GYYPNAEESEIHMLLNCKREPVSAGTILLKDQEKPEHVILVLTGVAELLSTNDKTHFQ
                     LSSGTLIGDLPVLFGLKSTGTFRALTYVETLKIPAVLFKEFVNRHRLLGQIKKTQNTI
                     EFLRQTWLFGESISTPVQSQIAQKMKLRKYEKGASITCEGLMLVKDGKVELSDSGTEM
                     QNSRNEVVEKGDFWGCEQMILNKALNSNAIALASSFIYSIAETEILEQIPIVRWKLLE
                     QAHKRA"
     gene            complement(7037..7816)
                     /locus_tag="EYO68_03660"
     CDS             complement(7037..7816)
                     /locus_tag="EYO68_03660"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIC06932.1"
                     /translation="MKPFQYAIIGGTGVYDSGVSSFQKTVETEYGEVEVDIAEVKKNS
                     IVFLARHGKEHGTLPHRINYRANLKALQKLGVKQIFATAAVGSLNPDYPTGSLVVLSD
                     FLDMTKQRPLTFFEGGSEGAKHVNMDDPYCANLRKHLLEAAKRHDVQFKGNAVYICTE
                     GPRFETEAEIKMMREWGADVVGMTSVPEVVLAKELGLCYASVGFVVNMATGMESGPIQ
                     LESIGEMLARNKEKVNLMFLDIFTKTLDQQNCGCLDSIVNL"
     gene            complement(8431..9270)
                     /locus_tag="EYO68_03665"
     CDS             complement(8431..9270)
                     /locus_tag="EYO68_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06933.1"
                     /translation="MTFIVLSLVLFSALMHACWNLFLKQSEDRLVTMATIHLVSGAVG
                     MAAVPFLPLPCVESWPYIFASVVLHLGYQLFLVKAYVYGDLGLVYPIARGISPLLLGF
                     ITVFWIGEELTLQMMSAIGLISLGIFSLMFPGKMSIKYTFRPIIYALATGSFIAAYTL
                     ADGVGVRASGNAASYVLWLMALECLPLLGITLILRKRAVFSSIHKNWKYAVPGGFLSL
                     SAYWIVIWAMQHAPIALVSAIRESSVVIAALMSIWVLKEETTVKHLLSAVMVAAGVIL
                     TRL"
     gene            9414..10256
                     /locus_tag="EYO68_03670"
     CDS             9414..10256
                     /locus_tag="EYO68_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HIC06934.1"
                     /translation="MRTFLDRLHDLKLNEVALRKNEISENNLRVKVETLGPGPDFRNC
                     LLRKGNDPISLIAEVKSRAPGRKNVERLDPETVVCDFEKGGAKAISVLTDESWFGGSL
                     ETLEKVSAATTLPLLHKEFIVTSYQLLEGRIRGASAALILAYYFQETELRQIIDEAKK
                     IGLEAVVECSLEDELPRALAVNPDILMINNRAIAAIPEEPVETYDLGSIDVISQWWER
                     NPDLRTWKEQNDKLLISASCVHSVVDIQSLLKIPCDAALIGNAAMAAPDRIEFLRSLV
                     KTEN"
     gene            10282..10716
                     /locus_tag="EYO68_03675"
     CDS             10282..10716
                     /locus_tag="EYO68_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC06935.1"
                     /translation="MKTTANSKLSILVVDDDENIRMVLRQSLEKEGYHVSTANSSEEA
                     LNTLQRSFFHVVITDIMMGEMNGVELLLQIKEMNSLMQIYVMTSHGTLPHVIQCMQGG
                     AYDFFEKPLKIEDILISLGEAARRATRWSSLYSRHSLSAKGK"
     gene            complement(10735..11775)
                     /locus_tag="EYO68_03680"
     CDS             complement(10735..11775)
                     /locus_tag="EYO68_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIC06936.1"
                     /translation="MPDHKSLVMKQQNKAGIVQKTSPPQSLTQILKVVSSKIELLESQ
                     ILRDVLTDIPLLNNVAEHILSSGGKRLRPALVLLGAEMFGGIDERVMQAAQVIEYLHT
                     ATLLHDDVVDGAETRRARQAACRVWGNEVSVLSGDYLLAMAFHRLTKLRYPEVLELMS
                     ETTTRMARGELLQLTRSYKTANEDDYFEIIINKTACLFATAIKTGALLAGASGKSADL
                     MYDYGMAIGISFQIVDDALDYSDEKKTGKPVGGDLQERKITLPLSHLLEQANVRDRKR
                     LDLILAQAIIEKPQIEEVTCLMKRYGSIDYTLAHSREYAAKARQYIENFPDTELRQSL
                     AGIADYIVSRQD"
     gene            complement(11768..16351)
                     /locus_tag="EYO68_03685"
     CDS             complement(11768..16351)
                     /locus_tag="EYO68_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIC06937.1"
                     /translation="MGSNKKKTYKHHSAFHAAEVMSHDLSLLRGKKNSVYFDDSELLG
                     LLSAEEVEKEVQQLKKVDVDSYIQRVVSPSESKNRQSAPEVIQQLGGKMIAEETEGPL
                     YSAEIEISISGHIRRLGFIAQNHKIQNGAWSPNHHRKAAEKVRFFASHSIPLVTFIDT
                     PGAVADAEANLDNQSHSISFLISEMANLQLPVIGIVFGGGYSGGAIPLATANILLSVR
                     EGVFNTIHPKGLSNIARKYNLSWQESAKHIGVSAYELQSQGYFDGIIDYTYDQQHKVK
                     NIKDAIISAVEVTEKNSCNFLTENEFFFDHYRDSINHYLNPSKLLIESNRITDRSTTG
                     TLNIFGDVFRFMRYLKIRQRIVSRSIDSYARLSSRFTPVGKLQERLKNERQEKFKNWY
                     KTPLEIRFHEKLNKRFEQFVSAREDREKQRGKFVAFFIGDPQENYKKTTDDLTLEILL
                     FLYNYWKDSSAENLIELYEQLDKSTLHDLPPGNPNLLDLLRTIDINQSFKENFQNILL
                     FDLLYDQVIEALPTIADELKGSNQISQKSVEQLFEQTVEGAMKEFEKNSLQLKSKSVR
                     TQFFKWLASFIDRKDCDDVLGTISEWKRVVFPRMSPPLFGVVRYYFSGLLPSLYASQQ
                     GKGKFQGKITPRNIGIKDFWNRLDQSYKDLLIKNLLREYKSTVITSNKVIENYFKDFK
                     ELYADRITSNPVKFPGFRDAIEQALENGVTPCGVVTGLATFTGEENSENGSAKKQKTK
                     KQKATYRVGLVVSNVEFQAGAFDMASCEKVCRLLDDCARLKLPVIFFISSAGMQTKEG
                     VGSLFSMSIINERITRFVKDLDLPVLCFGFRDCTGGAQASFVTHLLARTYYLSGSQIP
                     FAGQLVVESHLPVHSTLSNYLSTNPGTMDGLVKNPFDTDIDEKLLEIDPQIPVAKFSV
                     EEIISRVLSGEYQLSIEDEVQAYSTQENLHVGEIQRVLIHARGCTAARLILGAQDAGM
                     EVVLVASDPDMESYPATLLKKEDRLVCIGGNTPQESYLNGMSVIRIAEQEEVDAIHPG
                     IGFLSESPHYARICREHGFNFIGPRVANMDRMGNKSNAIATAKNLKIPVVPGSDGALM
                     DPAQAMVVASEIGFPVLIKAAHGGGGKGIEVVHEVTKFQSIFSRMFQEALSAFGNGDL
                     YLEKYIGSMRHLEVQIIRDMHGNSKLLGIRDCSVQRNYQKLIEETASGIPKKIREQVY
                     NYAEKLIEEIDYIGAGTVEFIYDLTEKKIYFMEMNTRLQVEHPVSEMVYGVDLVRLQF
                     EVTQGYNIGELDYKLKGHAIELRVIAEKIELDDENELRFVPDPGHVSEVFFPKKANMR
                     VIQSVDSGSVVSPYYDSLIAQIICWGKSRREAINGLLKYLKGVKIHGVSTNLALNRAI
                     LQDASFQKGGFSTKYLVDFFEEVDSKTLLKEAQRDSGSTKSSVDQKAIMLEDSDELKV
                     LSPQMGGFYRATSPDDEPFVSEEQIIDVNHTLCLLESMKVFNSLTLADYKSPDGEVLY
                     PEGSKYKVIRVIAEDQNTVNKGDLLFVIQPVDA"
     gene            complement(16390..18297)
                     /locus_tag="EYO68_03690"
     CDS             complement(16390..18297)
                     /locus_tag="EYO68_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC06938.1"
                     /translation="MKLINLLLFFLLSILLLSGCSENAKNIQKFLLTKENVHVYIEKQ
                     KLIVGNDEENAAAHFNLARSYFFIKEYDSAENHARRATRFDPLNAAYYELLGSLAFVL
                     ERYGDAVTEFATAVRISPERVSSYLKLAATYEKIDDDGRAISSLEQALQVDRYYVEAL
                     YHLSRIYLRQREFEGSLRTIETLLKLEPQNKEALLMRVQTYSLQGSYYYAQTLADEML
                     VRFPDYLPVRRELLRIQFAQQQWPEAQALLIQLRTKNSLSPEDQLIEAYLLLHQEQGK
                     EARKRFKAILEKQPKNVDAIMGLAVQLLRRGFLDDSLTWLTRGLEINPSLARAHYLRS
                     SILFRQGDYLQGDLAIGRALEIDSANPSYQLLFLRRQLMKGKLAVVEKQLRRIQEKHP
                     LNPEALQLQADLYKSRGNSAQAEKLLRQAILVNDSPSLHFSLARVLYQQSKYRSVLEV
                     TTPLMEVLTGNWEVIYLHALTLSRVGNYEEALEISRPYLKRRESQGYAHRLVGDLLRY
                     KGEEKEAQKILRNGLVQFPGHLYLVDGLSSSLVMTEDWAEVQELLETTLEQSQRLEGN
                     PPMQLLFLDRLAVAYQRLKNTVKKLQILRKFHQKNDPLTAAQLHSLEEQLLFPISLPS
                     LDKALSPLLLP"
     gene            complement(18301..19437)
                     /locus_tag="EYO68_03695"
     CDS             complement(18301..19437)
                     /locus_tag="EYO68_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06939.1"
                     /translation="MNYFFTFNLGKFYFFSFLFCCGWLVSCGLRTSPHNLPEVIPKST
                     FTNFKVQQRDQRIRLSLTINETERKNALMKLDDDLDQQDYFLIQEQKLKIGCRDCEIE
                     KMPALRILFSSDSFIRQGDNLYYYLELPQSDLKIHQFQVSHFGPEDEILSPVKTVKFR
                     QSNRFPNVPVPNLHIVQIEDEKKIIRFSFGKVVLKKITVVDDEPEKLLEQKKDKAENP
                     ETIIQNKQQQIESRTFVLKLSWPILSYKGSKRLKGKGDYFEGQEFFKVHLYRTLSGEA
                     WPETPINVKTIPNNHYLDRLKYFIPPATYPHMADTHPDISPPKLSFYIDLHGQKRDTW
                     LYNLRLVDRFGNESAASETIKFQSPETMILGQNFGQKIFVPLSD"
     gene            complement(19441..20376)
                     /locus_tag="EYO68_03700"
     CDS             complement(19441..20376)
                     /locus_tag="EYO68_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HIC06940.1"
                     /translation="MSLLICIPDSMQPQSLCSALQSLDSGLKIEIGSENVHNQDEVEF
                     AVVWKHPEGLLRNYPNLKAISSYGHGADALLADKQLPAGVPLVRLMDTTMAKWMSEYL
                     LTVVLLQRRQLLEYAKNPEFSAWGTAVCHSHPGNQIGILGLGYLGLAAAKVFLLMDFK
                     VSGWSRRKKQVKEVESFSGKKGFSAILKQTDYLINLLPHTTETRDLLNIETLSQLKRG
                     AYLINVGRGQTLVDEDLIALLDGGQLSGACLDVFRTEPLPKEHPFRKHKNILITPHNS
                     SATPAESVAPQILENYRRAVSGRQLLNTVDLEHGY"
     gene            complement(20423..20911)
                     /locus_tag="EYO68_03705"
     CDS             complement(20423..20911)
                     /locus_tag="EYO68_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIC06941.1"
                     /translation="MEGRIKSIAEFRLIPEYLTNISDEGKNWLARAIVNILIVDKQLA
                     PEEKGFFKDAIMMVESEKVRSELIESIKNRETVELGELLSDRDYAGHFFFFLGMVIAA
                     DGKIKTSEVKMLTSICGKLGFPSETAKTVLSWVSNMIKLNNEKNKLTVVMSEIKPVFV
                     LK"
     gene            21152..21775
                     /locus_tag="EYO68_03710"
     CDS             21152..21775
                     /locus_tag="EYO68_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06942.1"
                     /translation="MSSSPDQIKEELLHIEIAVELDQGKSTNEVAEKFGIKLLVVKAI
                     AKIIAADQPQTKTVKSRRFSEAEREVLVGRIEAGETVEDIGAEAGVTENTLRRWCKLR
                     GITPPRILEQISIAEQREIRELLEEQVWQDIAQAYNVSRDAIEELKEPAHRQLDTETL
                     SYLFELLREQPAAASKKLCGIAKEAGLDIPENAVSSYRKRLKSLNII"
     gene            complement(21772..22089)
                     /locus_tag="EYO68_03715"
     CDS             complement(21772..22089)
                     /locus_tag="EYO68_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06943.1"
                     /translation="MSTENGVRLQVDQQKRIGVSDVITMLKGLRSEAVIKIVRTSDAK
                     RKADTLNFGNDDGKDGSENSTHSFNQSSAVKGKTELPQIRELNEIAALLRSMDDQQNQ
                     LGS"
     gene            complement(22294..25326)
                     /locus_tag="EYO68_03720"
     CDS             complement(22294..25326)
                     /locus_tag="EYO68_03720"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06944.1"
                     /translation="MRKILTLFLAICFLAGASISVWGGGLNNTDPRYPQRQYRRFLLP
                     NQMKVMLISDPTLQRGAASLTVAVGSMSDPSGRSGLAHFLEHMLFLGTEKYPDEGSYQ
                     EFVSTHDGFSNAYTANDRTNYHFEVDPDYFEEGLDRFSQFFLAPLFNSGLVEREMKAI
                     DSEHSKNIPNDFRRIFQVKRKAFEKGHPARHFATGTLKTLAGVSRKELLYFYQKHYSS
                     NQMMLAVAGPQDLDTLQSWVVPRFVSVKNRNLQEIRVSAKYMKRDPRFRLMRIKTIKD
                     SRSLTLMFPLSRTLHYYKSQPLGMLGFLLGHEGKGSLLSLLKRENLAAGLSAGGGDSN
                     KSFSSFDVKIQLTPKGLRNYTKVIRRVFQYLRLLRETGLPRYIYEEVKLMSEIDYKFA
                     EKPEGTSLVNVFSTLMMYYPMRKLEVDPYIITEFKPRIFDSMLYSLTPENMLAILAAR
                     DVKTTEKEEYYGVEYSLTYSNPKWVKNWRNSKKLSALKLPEPNPFLPENLGVLPFEGT
                     VQLTHQSLVGLQQDGLSSEVLNRLKPLQGKLWKSLDELLTSAEFKPETDLAALRELLR
                     KHALGNPETIQDDEFGKIWFQQDFRFETPKAHLMFRIHSPHVYSSPRNAVLSQLYTDA
                     VREGLNEFGYPVSLAGLEYGINVDKKGINLTFSGYSDRIQELVKKVAGRLKTITIDKK
                     TFNTLKEARLRRYRNFHFQQPYQQAFYFRSLLLEGKKFSIMDYEKEIKKIRLHDVNKF
                     AKKIYDRLFIEGFAYGNLRAETVREAAKVLREKLGGKILPEVNRFLGSVRQLPQGKSH
                     TFTRKMQVENSALVTDVQVGQRSPKLQAALMVIDNLMQPQFYNELRTSQQLGYIVNSG
                     MTVLKKTLGLIFIIQSGEYNTETLEQRMEAFLEKFYSSLKNLTDQELNKIKKSVLHSK
                     LQKSTSVTGEAGRLFTIAFDRNAEFDKNSRGIKALEKLTPEDIQNVVSSYLLPSKQRK
                     LILRMSGKDHESGESSGEMISSIAKFKDQYACPQSCLP"
     gene            25771..26094
                     /gene="cyaY"
                     /locus_tag="EYO68_03725"
     CDS             25771..26094
                     /gene="cyaY"
                     /locus_tag="EYO68_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIC06945.1"
                     /translation="MENKEYFVRVSELFVRVEEKLDEFEDEIDYDPTPDKLMVTFENN
                     SEKIVINTQRAIKEIWLAGNSRGWHFQFLQEKDIWFANAEQEEFYQCLAKLLSENLGT
                     SVSFE"
     gene            complement(26127..26615)
                     /gene="moaC"
                     /locus_tag="EYO68_03730"
     CDS             complement(26127..26615)
                     /gene="moaC"
                     /locus_tag="EYO68_03730"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIC06946.1"
                     /translation="MPDSKNEFTHLSVNDQPRMVDISEKSVTERKAVAVARVHLGAEL
                     AALLKDTGSTKKGPVLQTAVIGGIQGAKRTSELIPMCHPLPLSGVNVDIELKGEYAFI
                     QATAKTTGRTGVEMEALTGASIAALTLYDMCKSVTKSMEIESVYLVEKTGGKSGNYRK
                     KD"
     gene            complement(26646..27347)
                     /locus_tag="EYO68_03735"
     CDS             complement(26646..27347)
                     /locus_tag="EYO68_03735"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIC06947.1"
                     /translation="MSGSQIKLYQTDNGPCPYRDNYTWHNISFQTSEIPADGYTSLLN
                     QGFRRSGLSIYHPVCSGCQACIPIRIDVQKFSQGKGQRRTWRKNKDVRVEHHPVGFNQ
                     EDFNLYQRYQIDWHEIKYPVEESEYFDFLIETPVPTEILRYYLGTKLIGVGWLDCLPE
                     LISSVYFVFDPEFESRRLGIFSLLYEIEYARFLDIRWLYLGYWVESSHKMNYKADFQP
                     AQILKDSRWIPFKNQ"
     gene            complement(27391..28362)
                     /gene="ftsZ"
                     /locus_tag="EYO68_03740"
     CDS             complement(27391..28362)
                     /gene="ftsZ"
                     /locus_tag="EYO68_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIC06948.1"
                     /translation="MTEKQTTQFSPVQEAFIPNIKIIGVGGAGGNVVTQMAEENLDQV
                     ETIICNTDVKALKRNKSHKKIELGKKLTRGMGAGARPEVGEGAAEESLAELSEAVEGA
                     NMVFIAAGMGGGTGTGAAPTIARLAKDKQILTVGIVTIPFSFEGTKRLDSGLAGVERL
                     REYTDTLLVIPNDKLLDASTQSTSFLEAFHMVDMVLINAIRGITLLLSGVGDINVDFA
                     DVETIMKGMGKAVIGRGVAKGKDRALNAVEDALHGSLMEDTDIRGAKGILVHVSGPKD
                     TSAYEIQEAMSLIEDMADEDVELIWGASFSDEAGDEVSMTVIATGLS"
BASE COUNT         8591 a         6089 c         5582 g         8574 t
ORIGIN      
        1 gaatctgagg aagatattct acgaactctg aaactggaac cgaggcactt tggagcactt
       61 tccggacagg gactgatacg gatggcattg gaggactggt ccggagccat cacggtactg
      121 gaagcaggac tgaggattca ccctcatatg cctggagtaa tcaggaattt ggaatatgtt
      181 cgaaagaagc agaaagaatc gatgacttaa ccggatattt taataataga attccaaaag
      241 gctgactttt caatatcttt tattaatcaa gtcaggccag ctaaaaccaa aaagtcaacc
      301 gattctcagt tgagcagttt tggggtctcc aggtaatcaa ccatatcgtc cagagctttt
      361 gcaaaaacat gagtagcata gtcaactttt gtattgtcgt caatcaagtg gctttccaat
      421 gaatccatca attccttgat ggttctggaa acctgtttct caaattgatg actaagatcc
      481 acgccaactg aattggcaaa attttctatt ttttcttctg tttgtgtcat ttcttcccca
      541 ttctcaaatg ccggtttgtg aaataaatca ttatgcctgt gcgtacctta gagaaatgtc
      601 tctatagatt tgcaaaatac tgtagctcta ggtgttaaat ttgcttgaac caggtagagc
      661 agttgaagct actctccaat cagttctata tatcggcatt ttttacaaaa aacttgagta
      721 ataaacaaaa gaataattca tgtacagcca actaagtcat ttcaaagaaa gaatagatga
      781 aacttttgaa atcatttttt ctttcaggaa accagccgca gtattaatat ttttatggat
      841 tggaatcagt tctgttgagg cacaagaata tgccaccgat cggctattca tgaaagagta
      901 cagtaaggcc aagtgccgta acgaagtaga aaataagatc agacgtttga aaaacaacgt
      961 ggatatgact cttgagcatc aggcattttt aaatcgaaat atctggtcaa agttacatac
     1021 aaatttaccg ctcagtcgtg gtgaaaagaa acacctcaac gatctcaaac aaaaaggaat
     1081 acccctaaaa aaaataagat ctaaggatta ttgggcttac aatgctgcac aattccgggc
     1141 gcttcgatta aaatgcaaat aagtatcatt tactgatctg acttgcattt ccccccattt
     1201 ctcttgataa tacaacgctg atattcaacg catctttgtt tgcgctacga aaaatattct
     1261 tcatttttca ctcatctatt tagtgctaac ttgaaaaagt atttcttttg tcatcccgga
     1321 cttgatccgg gatccagata cacgaccgga ttaaaaacaa gataataaac cacactcctg
     1381 gattccgggt ctacgctgcg ctccgcccgg aatgacaacg gagaagaatc gaatacggaa
     1441 aaactgttat tcccgtagag acagcttgca ggatgaggaa atatagcaac atttgttcct
     1501 ggctttttac ggtttcagga taagcatcta cacaagacat cctgaattga ataattgtct
     1561 atttaaatta ttgactaaaa tttatattca gcaaaaccta tttacctcaa cacgaatatt
     1621 aggattattt tatggcattg caaacacccc tgagttggct gcaggatttt gtagaactga
     1681 atcagtctcc ggatgatttg gcagagcgct taactacagc aggacttgag gttgaaacca
     1741 tagaaaaaat aggagaaaac tggggagaat tttgtgttgt aggtcaaatt aagaaaatcc
     1801 gcaagcaccc aaatgccgat gccctgcatt tagtggatgt tgaatttggc gcagacaaac
     1861 caattacact tgttacaggt gcgccaaaca tccgtgaaat gggaaattca attccggaac
     1921 ctgctcctaa agttgcactt gccctgaccg gggctatgct gattgatgcc taccacgaag
     1981 atcgtgcgct aaaaaaactt aaacctgcta aaattcgcgg tattgcttct gaaggaatgc
     2041 tttgctcaga actggaactt ggactcagtg aagaacatga aggcattttg attcttcctg
     2101 atgatgcacc ggttggcaca ttgctgcagg attttttagg cgacactatc ctgcatttcg
     2161 atataaaagg cggtttcagc cacctccttt ctatgcttgg agtagcacgt gaaattgcag
     2221 cgcttaccgg tcagaaatta gacaaaagtg tgctgccgga tctttcaaga acagaagttc
     2281 tggaacaacc tccgtttgtt gaactggaaa ttaaggatcc ggatatttgt ccgcgttata
     2341 cggccctgtt aatccggaat gttaaaatcg gaccttcacc gttttggatg cagcaacggt
     2401 tgttgaggat tggaatgcgt cctatcaaca atattgttga cataaccaac tacgtaatgt
     2461 tggaattggg tcagccgctg cacgcctttg attacgacaa actgattgaa cgggcaaacg
     2521 gagatacacc aaaaataatc gtgcgtcgtg caaaaaaagg tgaaacactt ctcaccctgg
     2581 attatgttga gcggaaattg gactcagaaa tgctgatgat aacagattct gcaggactaa
     2641 ttgcaattgc aggagttatg ggtggcggtg attcagaagt tactgaaaca acaaaaaatg
     2701 ttctgctgga atcagcaaat tttgaattta ttaacaacag acgtacttcg caggttctca
     2761 aattaagaac tgaagcaagt gaacgcttcg gcaaacaaat tgatcctgaa ggaacattgc
     2821 aggctgcatt gcgtgccgca cttttgatgg ttgaaaatgg ttccggaact cttgagaaga
     2881 ttgccggaga cctctatcct cgacctaaag aaaccgtttc actggaactg gatccgtttt
     2941 atgttgaacg cctgctggga ataaaaattt ctgctgatca aatttcagaa attttaaatt
     3001 cactggaatt taaggtttca gggaaaaaca ttttaaaaat tacagttcca agccatcgaa
     3061 tggatatccg aattcctgca gatctggttg aagaaataat cagaatttac ggctacgaaa
     3121 atctgccttc aacactgctc gatgatgaac tcccaccgca gcacagcaac caaaaacttg
     3181 aaggcacaga acgtgtccgg gatattctag tggcttctgg aatggacgaa ataattactt
     3241 attccatgat ggatccgatg gatgaggccc gtttgcgtct tgaaaatgat gttgattttg
     3301 ctaaatttgt accattaaaa aatccactta ctcaagaacg aagccacctt cgtcgttcgc
     3361 ttttgccggg agcattacat acggctcatg cgaatttgcg atttttagat aaagttgtca
     3421 tgtttgaagt tggcagtgtt tttcatcctc aaaaaggcaa aaaacttccg gaggaaccgc
     3481 agcggttgtc tttgttaatg tgcggcctcc ggaattctca atcctggctg gaagaaactg
     3541 agggcaccta tgactttttt gatctgaaag gtgttttgga acattttctt tcggcattgc
     3601 atattgaagg aattgagtgg aaaaaatcca gggagttgcc atgccatccg ggacgctgtg
     3661 tgcagatttt ggtgaatgga aaagaattag gattttcagg tgaattacat ccaaaaatac
     3721 gcagtgcttt tgagttgcct gaacaagctg tttgtattgc agaactggac ctggatttga
     3781 tcatcaagct tggaattgag aatcaccaga tggactttat ttccaacttc actccaatct
     3841 ttgaagacct cgcttttgtg atggattcca gtttacctgt tgaagctatc acaccagtta
     3901 tcctccaaac cggaaaacct ttgttgcgca aagcaacact atttgatgta tatgaaggtg
     3961 aacaggtgga tgaaggaaag cgcagtttgg catattcgct cacttttcag gcaaacgaca
     4021 ggacactaac agatgaagaa gtagggaaga tccgcaacaa aatagtcaaa cgcctgcaac
     4081 atgagtttcg ggcaacctta agagcttgag gttaaatatg atgaaaacct tacttcattt
     4141 acggaaagct tttggccata gcatgaaggg actgcgtgaa actttccgca acgaaatggc
     4201 atttcgcata gaattaactg ctgctgtcat tttgatacct actgcactaa ttttatcagt
     4261 ttcaccgatt gtccggatta tgctggtcgg ttcagttttt ctggtattaa ttgtagaatt
     4321 acttaatact ggaattgaaa ctgttgttaa tagaatttct gcagaacaac atcatctatc
     4381 cggaatggca aaagatgctg gaagtgcctc ggtttttgtt gcctctttaa acctcgtttt
     4441 tgtttagggt acagttttgt ttgacttaat tcttaataat tattagattt gtgaaaatta
     4501 atttgatttt ttatgctgtc ctccaggaaa ctatttctga agatccatta ttgctggagg
     4561 tcaaaaaaga ctgcactgca gaacaggttc tggaactgct tgcagagaaa tttccgcagg
     4621 ctgcacctat tttaaaatcg accagactgg caaatgaaga cgagtacgtt ggtaagcaaa
     4681 ctttactaac agacggcgca gaatattgtt tgatacctcc tgtcagtgga ggatagtttc
     4741 atagtaatca atgatcattg tttgcagtaa ccaggatgtc caggattgct aatactaaaa
     4801 ttgccacagg tgtgttttgg gtggaagttc cagaggcgga attatacgtt ctttgcggat
     4861 gtccggctga tagtgtaaag catttgatga aggccggtaa aatccgtaat cttgatagag
     4921 atgttggttc gctggagcct gggtcagttt cctttcaaca tcaccacggc acagtgacaa
     4981 atgaaactgg tccgaatgct attttactct ccgatttaaa cattcaaaat ggtgactttg
     5041 ctaatttagc ggagtttcct gttctgcaga tgctttaccg tcaaggaatg cttttgccga
     5101 atcaccccaa caacaccggt gcaaaaccat tcatcatagg acacaagaac atggttaatg
     5161 ctcagatgga gtatattcat cgaggcaact acggtctgac aagtctggag gaaatccttg
     5221 atgctggtat tcctcagaaa caagccgaag aaatgatgcg aatcaagcta cactttgcat
     5281 ttggtgcagt tcgtcccagt tctgacctgc tagaagctcg aatcgttgat catgaacctg
     5341 tcgaaatcct gaacggagtt catatagcca gaaaaagcat gaactgctac gaatttactt
     5401 ataaagatga aagttcagaa ataaatctga acctgagtca tgatgaaaga tacgagactc
     5461 cctacgaact taaaaaccac cactttaaac gtgattactt ctccatcgca catacgggtg
     5521 agggagacgg ttgggatata aaccggccct gcatggccag cgttattagt tatcagggaa
     5581 aaatcttttt gatcgatgca ggccccaaca ttgccctcac cctgaatgct ataggtgttg
     5641 atgtaaatga agttgagggt atttttcata ctcatgcaca tgatgatcat tttgccggcc
     5701 tgacaactct tgcacgtgca aatcacagga tcaagtatta ctcgacagct ctggttcgtg
     5761 catctgtaac aaaaaaattg tccccacttt tgtcaatcag tgagaatgaa ttcgaaaaat
     5821 attttgaggt ttgcgatttg gtgtttgaca aatggaacaa catagacgga ctggaagttc
     5881 gaccggtatt ctctccacat cctgttgaaa caaatattct gtatttcagg acactgtggg
     5941 aaaacggtta tgcgacatac gcccatttag cagacattgc atctcacgat gttttgacga
     6001 agatggttga ggtggataaa aaactccccg gcataagtcc aaagttaaag aaaaaagtct
     6061 gggaagatta tcttagtcct gtacaagtaa aaaaaattga tatcggtgga ggaattatac
     6121 acggcaaagc aaaagatttt ttgactgata aatcagacaa aattatttta gcacacaccg
     6181 cacacacgct gacaaaagat gaagaaaaga ttggctgcgg tgtcactttc ggtagtactg
     6241 atatactgat cgaagggcat gaggattatg ctctgcaggc aggcggaaat tatttgcgtg
     6301 gttattatcc aaatgcagaa gaaagcgaaa ttcacatgtt gctgaactgt aaacgtgaac
     6361 ccgtcagtgc cggaactatt ctacttaaag accaggaaaa acctgaacat gttattctgg
     6421 tacttacagg cgtagcagaa ctgctttcta cgaatgataa aacacatttt cagttatcca
     6481 gcggaacact aattggagac ctgcctgtac tttttggcct aaaaagtaca ggtacttttc
     6541 gtgccctgac ttatgttgaa accctcaaaa ttccagccgt tctgtttaaa gagtttgtaa
     6601 accgtcaccg gcttttaggg cagattaaaa aaactcagaa tacaatcgag tttttacggc
     6661 agacctggtt attcggcgaa tctatatcta ctcccgtcca aagtcagatt gcacaaaaaa
     6721 tgaaactcag aaaatacgag aaaggcgcct caataacttg cgaggggctt atgctggtta
     6781 aagatggtaa agtggaatta tcggacagtg gaacggaaat gcagaacagc cggaatgaag
     6841 ttgtggaaaa aggggatttt tggggttgtg agcagatgat cttaaataaa gccttaaact
     6901 caaacgcaat agctcttgcg tcttcattca tctacagtat cgcagaaact gaaatcctgg
     6961 agcagatacc gattgtgcgc tggaaattgc tggagcaggc acataaacgt gcataaaaag
     7021 tcttaaccaa ataattctac aaattcacaa ttgagtctaa gcatccgcaa ttttgctgat
     7081 ccagtgtttt tgtaaagata tccagaaaca ttagatttac tttttctttg ttccgggcca
     7141 gcatttcccc aattgactcc aattgaattg gcccactttc cattccagtt gccatattaa
     7201 ccacaaaccc tactgaagca tagcacaacc ccagttcttt cgccaaaacc acttccggaa
     7261 cacttgtcat tccgactaca tccgctcccc attccctcat cattttgatt tcagcctcag
     7321 tttcaaaacg cggtccttcg gtgcagatat aaacagcatt ccctttaaac tgaacatcat
     7381 gtctctttgc tgcttcaagt agatgtttcc tcagattggc gcaataagga tcatccatat
     7441 taacgtgctt tgctccttct gatccaccct caaaaaatgt aagtggtcgt tgtttagtca
     7501 tatccaggaa atcagaaaga accaccagcg aaccggtagg gtaatccgga tttaaagagc
     7561 ctactgcagc agtagcaaaa atctgtttaa ctccaagctt ctgcagtgct ttcaaatttg
     7621 ctcggtaatt gatgcggtgg gggagggttc catgctcttt accatgtcga gcaaggaaaa
     7681 ctatagaatt ttttttaact tcagcaatat caacttcaac ctctccatat tccgtttcca
     7741 cggttttctg gaatgaagag acgccactgt cataaactcc agtgccacct ataatggcat
     7801 attggaaggg tttcatgaaa ttttaatgta gggcgacggg actttataac aaatactttg
     7861 caagtaataa tctaaccaaa aaaatccgag tcgtgcattt caatccactc aaacatatct
     7921 ccagtcagcc aagtttcgct tgccaaaatt ggctcgccga taactcgctc gtagcggctt
     7981 accaaaaatc gggccattgc ctcattgctt tgttcgcttg taacactcat ttttttctcc
     8041 tgtaattcat agtatataac tataaatttg ctgtatgggt taataattaa taaaaataat
     8101 ttctgacgtc ggaacgacta tcccagaaaa tctgagtctt gtgtttcaat taactctaat
     8161 gtatcctctg tcagccagat atcacttccc aaaattggtt cgccgacaac ccgctcatag
     8221 cgacgtattg aacctccccg atcaagaaat cgagtcattg ctgtattgat ttgatctcgt
     8281 gtaatactca ttttgctacc tcgttaagta attgatataa taataattat caccattaat
     8341 gaaaaattat ttaatggtgg ttatctataa atttgcaata tacagaccag aaaaatctat
     8401 tgttgattat taagaggaaa ttgaagcatc tcataatcga gtcaatatca ctccggctgc
     8461 aaccattaca gcagacaaaa gatgctttac tgtcgtttcc tctttaagaa cccaaatact
     8521 catcagggcc gcaattacca cgctgctttc ccttattgca gacaccaggg caatcggtgc
     8581 atgctgcatg gcccaaatga caatccaata tgcagacagt gacaaaaatc caccgggtac
     8641 agcatatttc caatttttgt gaatagaact aaaaactgca cgtttgcgta gaatcaacgt
     8701 tattcccagc aaaggcaaac actctaatgc catcagccat agaacgtatg aagcagcatt
     8761 tcccgaggcg cgaacaccca ctccatctgc gagagtatat gctgcaatga aacttcctgt
     8821 ggccaatgca taaataattg gacggaatgt atactttatc gacattttcc ctggaaacat
     8881 gaggctgaat atgcccagag aaattaaacc tattgctgac atcatttgca aagtaagctc
     8941 ttctccaatc cagaacacag taataaaccc cagtaaaagt ggcgaaatac ctctcgctat
     9001 tggataaacc agacctaaat caccatatac ataggctttg actaaaaata actggtaacc
     9061 taaatgcagc accactgatg caaaaatata cggccagctt tcgacgcagg gcaatggtag
     9121 aaacggtact gctgccattc caactgctcc ggaaacaaga tgtattgttg ccatcgtcac
     9181 caggcgatct tcactttgtt ttaaaaacag attccagcat gcatgcatga gagctgaaaa
     9241 aagaaccaaa gataaaacaa taaatgtcat aatatatcta taaaaagtaa atggaacagt
     9301 ctctcgacaa tttgaatgaa atgctattta acggcatatt gattgtatta ccatttgttg
     9361 atcgcagaac aagttctgct gatcagcatt tatttttttg aaatttaaaa tcaatgagaa
     9421 catttttaga cagattacac gatcttaaat taaatgaggt agcactaaga aaaaatgaaa
     9481 tttctgaaaa taacttacgt gtaaaagtag aaacactagg accaggccca gattttcgta
     9541 attgtctgct tcggaaagga aacgatccaa tatcactgat tgctgaagtg aaatcgaggg
     9601 ctcccggacg caaaaatgtt gaacgtcttg atccggaaac tgtggtctgt gattttgaaa
     9661 aaggaggtgc aaaagccatt tctgttctta ctgatgaaag ctggtttgga ggctctcttg
     9721 aaacactcga aaaagtgagt gcggcaacca ctttaccctt gctgcacaaa gagtttattg
     9781 taacttccta ccaactgctg gaaggacgaa tccgcggtgc aagtgcagct ttgatattag
     9841 cttattattt tcaggaaaca gaattacgcc aaataataga tgaagcaaaa aagattggtc
     9901 tggaggcagt agtagaatgt tctctggaag atgaattgcc tcgggcactt gcagttaatc
     9961 cggacatttt aatgataaac aatcgagcca ttgctgctat tccggaagaa cctgttgaaa
    10021 catatgatct tggcagtata gacgtaattt cacagtggtg ggaacgcaat cctgatttaa
    10081 gaacatggaa ggaacagaac gataaactat tgatcagcgc aagctgtgta cattctgtag
    10141 tagatatcca aagtttactg aaaataccat gcgatgctgc cctgatcgga aatgccgcta
    10201 tggctgcacc tgatcgtata gaatttttac gatcgctagt caaaacagaa aattaatatt
    10261 taatgtcata agaagaacgt catgaaaaca acagctaatt ctaaattaag tattttggtg
    10321 gtggatgatg acgaaaacat ccggatggtg ctccgccaat ctttggaaaa ggaaggttat
    10381 cacgtcagca ccgccaatag ttcagaggag gcactgaaca ctttgcagcg cagtttcttc
    10441 cacgttgtaa taacagatat tatgatgggt gaaatgaacg gcgtggaatt attacttcaa
    10501 atcaaagaaa tgaattcatt gatgcagatt tatgtgatga catcacacgg cacattaccg
    10561 catgtcatac aatgcatgca gggtggtgcc tatgattttt ttgaaaaacc gctgaaaatc
    10621 gaagacatct taatttcact tggagaagca gcccgccgtg caacccgctg gagttcactt
    10681 tacagtcgcc attctttgtc tgcaaaaggg aaataggata ttataacagg tatttcaatc
    10741 ttgacgagaa acgatataat cagcaatgcc tgcaagtgat tgtctaagct ctgtatccgg
    10801 aaaattttct atgtactgcc gagcctttgc tgcatattcc ctggagtgtg ccaatgtata
    10861 gtcgatggaa ccgtagcgtt tcatcaaaca tgttacctcc tctatctgcg gtttttcgat
    10921 aatcgcttga gcaagaatca aatccagacg ctttctgtcc ctaacatttg cctgttccag
    10981 caaatgactc aaaggaaggg ttatttttcg ttcctgtaaa tctccaccca ccggctttcc
    11041 cgtttttttc tcatccgaat aatcaagagc atcatccaca atttgaaaag agattccaat
    11101 tgccattccg taatcgtaca tcagatcagc agattttcct gaagctccag ccagtaaagc
    11161 ccccgttttg attgctgttg caaaaagaca ggctgttttg ttgataatga tttcaaaata
    11221 atcatcttcg ttggccgtct tgtatgaacg tgtcaactgc aataattcac cacgtgccat
    11281 ccttgttgta gtttcagaca tcagttccag aacttccgga taacgcaatt ttgtaaggcg
    11341 gtgaaatgcc atggccaaca gataatctcc ggaaaggaca ctcacttcat ttccccatac
    11401 tctgcaagcc gcctgcctgg cccttctagt ttccgcccca tcaacaacgt catcgtgcaa
    11461 caatgttgcg gtgtgtaaat attcaataac ttgagctgcc tgcataactc gttcatcaat
    11521 gcctccaaac atctccgccc ctaaaagtac aagtgcgggg cgcaaacgtt ttcctcctga
    11581 acttagaata tgctcagcaa cattgttaag taacggaata tcggtcagta catcacgtaa
    11641 aatttgtgat tccagcagtt cgattttaga agagactact ttgagaattt gcgttaaact
    11701 ttgcggaggg gaggtttttt ggacaattcc tgctttattt tgttgtttca tgacaagtga
    11761 tttgtgatca ggcatccaca ggctgtatca caaacagtaa atcacctttg ttgacagtgt
    11821 tttggtcctc tgcaattact cgtataactt tgtattttga cccctccggg tataatactt
    11881 ctccatccgg gcttttataa tcagccaaag tcaaactatt gaaaaccttc atcgattcaa
    11941 gtaaacaaag ggtatgattg acgtcaatga tttgttcttc actgacaaat ggttcatcat
    12001 ctggagatgt tgcacgatag aaacctccca tctgtggaga aaggaccttc aactcatcag
    12061 aatcttccag cataatagct ttctggtcca cagaactttt tgtactgccg gagtctctct
    12121 gggcttcttt caatagagtc ttcgaatcaa cctcctcaaa aaaatctaca agatatttgg
    12181 tactgaaacc tcccttttgg aaagatgcat cttgtaaaat agcacgattc aaggccaggt
    12241 tagttgaaac accgtgaatt ttcactcctt ttaaatattt aagtaaaccg ttaattgctt
    12301 ccctgcgtga ttttccccag cagataatct gtgcgatcag gctgtcataa taaggtgaga
    12361 caacagaacc tgaatcaacg gattgaataa cacgcatatt ggctttttta ggaaaaaaga
    12421 cttcagaaac atgtcccgga tcaggaacaa atcgcagctc attttcatca tccaattcaa
    12481 ttttttctgc aatcacacgt aattcaatgg catggccctt cagcttataa tccaactctc
    12541 caatgttgta accttgtgtc acttcaaact gtaagcgaac caaatcaacc ccgtaaacca
    12601 tttcagatac cggatgttca acttgaagcc gtgtattcat ttccataaaa tagatcttct
    12661 tttctgtaag atcgtaaata aattcaacag ttccggcgcc tatgtaatca atttcctcaa
    12721 tcaatttttc tgcatagtta taaacctgtt ctctgatttt tttcggaatt ccagaagcag
    12781 tttcttcaat cagtttttgg tagttacgct gaaccgagca atcacgaatt cccagcaatt
    12841 ttgaatttcc atgcatgtca cggattattt gaacttcaag atgacgcatt gagccgatat
    12901 acttttccaa atacaaatct ccattaccaa atgcacttag agcttcttga aacatacggg
    12961 agaaaatgga ctggaacttc gtcacttcat ggactacctc aatccctttt cctccaccac
    13021 catgtgctgc tttaatcagt actggaaagc caatttctga tgcaactacc atggcttgtg
    13081 ctgggtccat taaagcaccg tcactacctg gaacaaccgg gatttttaga ttctttgcgg
    13141 tcgcaatagc atttgatttg ttgcccattc tatccatatt agccacacga ggaccaataa
    13201 aattgaaacc atgttcccgg caaattcggg cataatgagg agattctgac aaaaatccaa
    13261 tccctggatg gattgcatcc acttcctcct gctcagcaat cctgatcaca ctcattccat
    13321 ttaaataact ctcctgcgga gtgttccctc caatacagac caaacggtct tctttcttaa
    13381 gcagtgtagc cggatagctt tccatgtccg gatctgaagc caccagaacc acctccattc
    13441 cagcatcctg tgcaccaagt atcagtcgcg cagccgtgca acctcgtgca tgaatcagca
    13501 cacgttgaat ttcaccaaca tgcagatttt cctgagtgga ataggcctgt acttcatctt
    13561 ctattgacag ctgatattcc cctgataata cacgtgaaat gatctcctct actgaaaatt
    13621 ttgcaacagg aatttgtgga tcgatctcca gtaatttttc atcaatatct gtatcaaatg
    13681 gattttttac aagaccatcc attgttccag gattggtgga gaggtaattg gataaagtgg
    13741 agtgtacagg caagtggctt tcaacaacca gttgtcctgc aaatggaatt tgtgatccag
    13801 ataaataata agtacgagca agcaaatgag tcacaaaact cgcttgtgca ccgccagtac
    13861 aatctcggaa accaaagcac aagaccggaa gatccaaatc ttttacaaaa cgggtaatac
    13921 gttcattaat aatggacatt gagaataacg aacccacacc ttctttggtc tgcattcctg
    13981 cagaagaaat aaaaaagatc accggcaatt tcagacgagc acaatcatca agcaggcggc
    14041 aaactttttc acagcttgcc atatcaaaag caccagcttg aaactctaca ttactgacca
    14101 ccaatccaac acggtaagtc gctttctgtt ttttagtttt ctgtttctta gcactaccat
    14161 tttcagaatt ttcttcacct gtgaatgttg caagtccagt cacaacaccg catggagtaa
    14221 caccattttc aagtgcctgt tcaatagcat ctctaaaacc tgggaattta actggattcg
    14281 aagttattct atcagcataa agttccttaa agtctttgaa ataattttca ataactttat
    14341 tggaggtgat gactgtactt ttgtattccc gcaacaagtt tttgatcaac aaatccttgt
    14401 atgattgatc gagacgattc caaaagtctt ttatgccaat attacgtgga gtgattttcc
    14461 cctggaattt acctttgcct tgctgagatg cataaagtga aggcagcaat ccggaaaaat
    14521 agtagcgtac tactccaaac agcggaggac tcattcgggg aaaaacgacc cttttccatt
    14581 cactgatggt tcccaataca tcgtcacaat ctttgcggtc tataaaactg gcaagccact
    14641 taaaaaattg ggttcttact gatttgcttt tcagttgcaa agagtttttc tcaaattcct
    14701 tcattgctcc ttccacagtt tgctcgaaaa gctgttctac agatttttga gaaatttgat
    14761 ttgagccttt cagttcgtcg gcaatcgttg gtagtgcttc aattacctga tcgtagagca
    14821 agtcaaaaag aagaatgttc tgaaaatttt ccttaaaact ctgatttata tcaatagttc
    14881 taagtagatc gagtaaattt ggattgcccg gaggaaggtc atgcagtgtg gatttgtcaa
    14941 gttgttcgta gagttctatc aggttttctg ctgaactgtc cttccagtaa ttgtacagaa
    15001 aaagtaatat ttccaaagtt aagtcatctg tcgttttttt ataattttcc tggggatcgc
    15061 cgatgaaaaa ggctacaaat ttgcctcgtt gtttctcacg gtcttcccgg gcactgacaa
    15121 actgttcaaa acgtttgtta agcttttcgt gaaagcgaat ttccagtggt gttttatacc
    15181 aattcttaaa tttttcctgt cgttcatttt tcaaccgttc ctgcaatttg ccaactggag
    15241 tgaaacggga actcaatctg gcataactgt caatagagcg actgactatg cgctggcgta
    15301 ttttcaggta acgcataaaa cggaaaacat caccaaaaat gttcagtgtt cctgtggtag
    15361 aacgatctgt tatcctgttg ctttcaatca aaagctttga ggggttgaga tagtggttga
    15421 tgctgtcacg gtagtggtcg aagaaaaatt cattttcagt taaaaaatta catgaatttt
    15481 tctcagtaac ttcaactgca cttataattg catcttttat atttttcact ttatgttgct
    15541 gatcgtaagt ataatcgatg atgccgtcaa aatatccctg agactgtaat tcatatgcag
    15601 aaacaccaat gtgtttggcc gactcctgcc atgaaagatt atacttgcga gcaatgttgc
    15661 ttaagccttt agggtggata gtattaaaga caccttctcg aaccgaaagc agtatattgg
    15721 cagtagccag gggaatagcc ccgccactgt aaccaccgcc aaaaacgatc ccgattaccg
    15781 gcaattgcaa attggccatt tcgcttatca aaaaagaaat gctgtgcgac tgattgtcca
    15841 aatttgcttc agcatctgca acagcacctg gagtatcaat aaatgtcacc agggggatac
    15901 tgtgtgatgc aaaaaaccgc accttttctg ctgctttgcg gtggtgattt ggagaccagg
    15961 cgccattttg tattttgtga ttttgagcaa taaaaccaag tctgcgaata tgccctgaaa
    16021 tagatatctc aatttctgca gaatatagtg gcccctccgt ttcttccgca atcatttttc
    16081 cacctaattg ctggattact tccggagcac tctgacgatt tttggattca gatggactta
    16141 ccacccgttg aatataggaa tcaacatcaa ctttctttag ttgttgtact tctttctcaa
    16201 cttcctccgc agagagcaac cccagcaatt ctgagtcatc aaaatacaca gaattcttct
    16261 taccccgcag aagagaaaga tcgtgtgaca tcacttcagc ggcatgaaac gctgaatggt
    16321 gtttgtatgt ttttttcttg tttgatccca tttaattatt ttatcttttg tcgtgtattt
    16381 gcggtatttt tacggaagta ataaaggtga cagtgcctta tcaagagatg gtaaagaaat
    16441 tgggaataag agctgctctt ccagcgagtg cagttgtgcg gctgtcagtg gatcgttttt
    16501 ctgatgaaat tttctcaaaa tctgtagttt ttttacagta tttttcagac gctgataggc
    16561 tactgccaac ctgtccagaa acaaaagttg cattggcgga tttccttcca gccgctgact
    16621 ttgctctaaa gttgtttcca gcaactcctg aacttctgcc cagtcttcag tcattactaa
    16681 tgatgagctc aatccatcta ccaaatacag atgaccggga aactgtacca gcccattgcg
    16741 tagtattttc tgggcttcct tctcttcccc tttatacctc agtaaatctc cgaccaatcg
    16801 atgcgcatag ccctgacttt ccctacgttt cagatagggc cgacttattt ccaatgcttc
    16861 ttcataattt ccaacacggc ttaaagtcaa agcatgcaaa taaataactt cccagtttcc
    16921 tgtaagtact tccataagcg gggttgtcac ttccaaaaca gaacggtact tactctgctg
    16981 ataaagtacc cttgccaatg aaaaatgtaa tgaaggagaa tcattcacca aaattgcctg
    17041 ccgcagcagt ttttctgcct gggcagaatt gccgcgactt ttatacaaat ctgcctgtag
    17101 ttgcagagct tccggattca gtggatgttt ttcctgtata cgccttaact gcttttcaac
    17161 aactgccaat tttcctttca tcaattgtct tcgtaaaaaa agaagttgat atgacggatt
    17221 tgccgaatct atttccaatg ctcgaccgat agccaggtct ccctgcagat aatcaccttg
    17281 ccgaaataga atggaggagc gcagataatg tgctcttgcc agacttggat tgatctccag
    17341 accgcgtgtt aaccaggtaa gagaatcatc cagaaatccc ctgcgtaaca actgtacagc
    17401 caggcccata attgcatcca cgttttttgg ctgcttttca agaatagctt taaaacgctt
    17461 acgggcttct tttccctgtt cctgatgtaa aagcaaatat gcttctatca gttgatcttc
    17521 tggtgaaagt gagtttttag ttcttagttg aatcagcaat gcttgtgctt ctggccactg
    17581 ctgttgagca aattgaatcc gaagtaactc gcgtctgaca ggtaaataat ccggaaaccg
    17641 aactagcatt tcatctgcca gtgtttgagc ataataataa ctgccctgaa gtgagtatgt
    17701 ctgaaccctc attaacagcg cttctttatt ttgcggttcc agtttcagca aagtttcaat
    17761 tgtccgcaat gaaccttcaa attcccgttg gcgcaaataa atccgggaca aatggtaaag
    17821 tgcttctaca taatatcgat ctacctgcag tgcttgctcc aaggacgaaa tcgcccgccc
    17881 gtcatcgtca attttctcat acgttgctgc taatttaaga taagacgaaa ctcgttccgg
    17941 agaaatccgg actgcagtcg caaactctgt gactgcatca ccataacgtt caagtacaaa
    18001 agctaaactt ccaagcaatt catagtaggc cgcatttaac ggatcaaaac gtgtggctcg
    18061 acgtgcatgg ttttctgcag aatcgtattc ttttataaag aaataggatc tggctaaatt
    18121 gaaatgtgca gctgcatttt cttcatcatt accaacgatt agcttctgct tctcaatgta
    18181 aacatgcaca ttttctttgg ttaacagaaa tttctgaata ttctttgcgt tttctgaaca
    18241 tccggataaa aggaggatac tcagcagaaa aaatagtaag agattaatca gtttcacagg
    18301 tcaatcagaa agagggacaa atatcttttg accaaagttt tgtccaagaa tcatagtttc
    18361 cggtgattga aatttgatag tctcagatgc agcactttca tttccgaaac gatcaaccaa
    18421 ccgcagatta tataaccaag tatctctttt ttgaccgtgc aaatcaatat aaaacgagag
    18481 ttttggaggt gaaatatccg gatgtgtatc cgccatatga gggtaggttg ctggaggtat
    18541 aaaatatttg agtctgtcca ggtagtggtt atttgggata gttttgacgt ttattggagt
    18601 ctccggccaa gcttctccac tgagggtacg gtaaagatga actttaaaaa actcctgtcc
    18661 ctcaaaataa tcaccttttc ctttaagacg tttcgagcct ttataggata gtatgggcca
    18721 ggacagcttc aaaacaaatg tcctggattc aatctgctgc tgtttgtttt gtataatagt
    18781 ttcgggattt tctgctttat cttttttttg ttccagtaat ttctcaggct catcatcgac
    18841 cacagttatt ttttttaaaa cgacttttcc aaaagaaaat cgaataattt ttttttcatc
    18901 ctcaatttgt acaatatgca agttgggaac aggaacattt gggaatcgat tactttgtct
    18961 aaatttcaca gtttttactg gtgacaaaat ttcatcttca ggtccaaagt gacttacctg
    19021 aaattgatgt atttttaaat cactttgagg aagttctaaa taatagtaca aattatcccc
    19081 ttgacgaata aatgaatcac ttgaaaataa aatgcgcaaa gccggcattt tttctatttc
    19141 acaatcccgg cagccaattt taagtttttg ctcctgaatc agaaaataat cttgctgatc
    19201 aagatcatca tccaacttca ttaaggcatt ttttctttca gtttcattga ttgtcagaga
    19261 caggcgaatt cgctgatctc tttgctggac tttaaagtta gtaaatgttg attttggaat
    19321 tacttccgga agattgtgag gagaagtccg taaaccgcat gagaccagcc atccgcagca
    19381 aaacaaaaag gaaaagaaat aaaatttccc taaattaaaa gtgaagaaat aattcatgga
    19441 tcaatagcca tgttctaaat caactgtgtt cagcagttga cgaccggaaa cagcccgtct
    19501 gtaattttca agtatttgcg gtgcaactga ctcagcgggc gtggcgcttg agttgtgagg
    19561 tgtgattaaa atattcttat gcttacggaa aggatgctct ttaggaagag gttctgtccg
    19621 gaacacatca agacaagctc cagaaagctg tcctccatcc agtaatgcaa tcaggtcttc
    19681 atctacgagt gtttgccccc gtcccacatt gatcaagtat gccccgcgtt tgagttgtga
    19741 taatgtttct atatttaata aatcccgagt ttctgttgtg tgagggagca aattaatcag
    19801 ataatccgtt tgttttaaaa ttgcagaaaa accttttttc ccagaaaaag actcaacttc
    19861 tttaacctgt ttttttcttc tactccaccc tgaaacttta aagtccatga gtaaaaatac
    19921 ttttgctgca gccagtccca ggtatcccaa accaagaatg ccaatttgat tacccggatg
    19981 tgaatgacat actgcggtgc cccatgcact gaattctggg tttttcgcat attccagtaa
    20041 ctgacgccgc tgaagaagta ctactgtcag taaatactcg ctcatccact tagccatcgt
    20101 tgtatccatc aaacgtacaa gagggactcc tgcaggcagt tgcttgtctg ccagcagtgc
    20161 atcggcacca tgcccatatg atgagatagc tttcaagttt gggtaatttc tcaacagacc
    20221 ttccggatgt ttccagacta ctgcaaactc aacctcatcc tgattatgta cattctcaga
    20281 accaatttct attttcaatc cggaatcaag tgactgcagt gcagaacaca aagattgagg
    20341 ctgcattgag tccggtatac aaattagcaa agacattcaa cactaaggtt aaagtaataa
    20401 ttcaaaatat tttcagagat acttacttta aaacaaatac aggttttatt tcactcataa
    20461 ctacagtcag cttattcttc tcattgttga gttttatcat gttggaaacc caacttaaga
    20521 ccgtttttgc tgtctcagac ggaaagccta gttttccgca aatggaagtg agcattttta
    20581 cttcggatgt ttttatcttt ccatccgcgg caattaccat tcccagaaag aagaaaaaat
    20641 gtcccgcata atctcgatca ctaagtaatt cgcctaattc cacagtttca cggtttttga
    20701 ttgactcgat cagttcgctt cgaacctttt cgctctcgac catcattatc gcatctttga
    20761 aaaaaccttt ttcctctggg gctagctgct tgtccacaat caaaatattt acaattgccc
    20821 gagccagcca attttttcct tcatcactga tatttgttaa atattccgga attaaccgaa
    20881 attctgcgat tgatttaatt cttccctcca tagtcacatc tccatgaaac agattagttt
    20941 atcaagaatg ttgttcatct tgaatccacg taatttacac cttccaaaaa acattaacgg
    21001 cccaaaaact agacttaaat gctgttactc agcatcgcaa aggccaagca tcttgtcaag
    21061 aatgtcttga ccctgttagc aaaaaggagc cttttaaatt gaataaatta aacaatgatt
    21121 aattttagta aaactctcag cagtatttac catgtcctca tcccctgatc aaattaaaga
    21181 agaactcctg catatcgaaa ttgcagtcga gttggatcag ggaaaaagca ctaatgaagt
    21241 ggccgaaaaa tttggaatca aattattggt tgtaaaagcc atagcaaaaa taattgcggc
    21301 tgatcagccg caaacaaaaa ccgttaagag cagacgtttt tctgaagcag aacgggaagt
    21361 tctggttgga agaattgaag caggtgaaac tgtagaagat ataggggcgg aagctggagt
    21421 tactgaaaat acattgcgcc gttggtgtaa actgcgtgga ataacacctc ccagaatact
    21481 tgaacaaatc agtattgctg aacagcgtga aattagggaa ttgctggaag aacaggtttg
    21541 gcaggatatt gctcaagctt ataacgttag ccgtgatgca attgaagaac tgaaagaacc
    21601 tgcgcatcga caactcgata cagaaactct aagttatttg tttgaactgc ttcgagaaca
    21661 acctgctgct gcttctaaaa aactttgtgg gattgcgaag gaagccggtt tggatatccc
    21721 tgaaaatgct gtgagttctt acaggaaacg gcttaaatca ttaaacatta tttaggaacc
    21781 aagctggttt tgctggtcgt ccatgctcct caggagagct gcaatttcgt taagttcacg
    21841 aatttgaggc aattctgttt tacctttaac tgcagacgac tgattaaatg aatgtgtgga
    21901 attttcactg ccatcttttc catcatcatt tccgaagttt agagtatcag ctttcctttt
    21961 tgcatcagaa gtacggacga ttttgataac cgcttcactg cgtaaacctt tcagcatagt
    22021 aataacgtca gaaacaccta tacgcttctg ctgatctact tgaaggcgaa ctccgttttc
    22081 agtactcatt tctcagtttt gtaattgttg tgacaataac acgtctcctg cctaatatca
    22141 ctcgcaaagt tcataccaca attcttgctc tatttttcta ttcctccttc tcgacaaaag
    22201 ttaagctaat ccggtttggt caaattcgta cttctaattc agtttttgat ttccgagaat
    22261 ttctattttt cctcttcgtt caaaagactg aaatcaaggc aggcaacttt gcggacatgc
    22321 atattgatct ttaaatttag caattgacga aatcatttca ccggaagatt caccggattc
    22381 atggtctttt ccggacattc tcagaatcag cttacgctgt tttgaaggca gcaaataact
    22441 gctcacaaca ttttgaatgt cttccggcgt gagtttttcc aaggctttaa tgcccctgct
    22501 gtttttatca aattctgcat ttcgatcaaa ggcaatcgta aagagtcgac cagcttcacc
    22561 tgttacagat gtgctctttt gtaatttact gtggagaaca gattttttaa ttttgtttag
    22621 ttcttgatcg gtgagatttt ttaatgaact atagaacttt tcaaggaaag cctccatcct
    22681 ttgctccaac gtttcagtat tgtattctcc tgactgtatg ataaagatca accccaatgt
    22741 ttttttaaga acagtcattc cagaattgac tatgtaaccc aactgctgac tggtacgtaa
    22801 ttcattataa aactgcggct gcattaaatt gtcaatcacc atcaatgccg cctgtagttt
    22861 tggactacgc tgtccaacct gaacatctgt caccaaagct gaattttcga cctgcatttt
    22921 acgagtgaag gtatgggatt ttccttgagg caattgacgg acacttccca gaaatcgatt
    22981 aacttccggt aagatttttc cgcccagttt ttcacgaaga actttagcag cttcacgcac
    23041 agtttctgca cgtagattcc cataagcgaa gccttctata aaaaggcggt catatatttt
    23101 ttttgcaaat ttatttacat catgcagacg tatttttttt atttcttttt cataatccat
    23161 aatcgaaaac tttttgcctt ccagcagtaa actccgaaaa taaaaagctt gttgataagg
    23221 ctgctgaaaa tggaaattcc ggtagcggcg cagacgagcc tccttgagag tgttaaacgt
    23281 ttttttatct attgtgattg ttttcaaacg gcccgcaact tttttgacca attcctgaat
    23341 cctgtctgaa taaccgctga aagtgagatt tattcccttt ttgtctacat tgattccata
    23401 ttctaagcct gccagactta caggataacc aaattcatta agcccttcac gtacggcatc
    23461 cgtataaagt tgtgacaaaa ctgcatttcg cggtgatgaa taaacatgcg gagaatgaat
    23521 ccggaacatc aaatgtgctt ttggagtttc gaaacgaaaa tcctgctgaa accaaatttt
    23581 tccaaactca tcatcctgta ttgtttccgg gttccccaat gcgtgtttac gcaaaagttc
    23641 acgtaaagcc gctaaatctg tttccggctt aaattctgct gaagtcaaca attcatccaa
    23701 cgacttccac agctttccct gcaggggttt taaacggttc agcacttcgg aactaagtcc
    23761 atcctgctgc agtcccacaa gtgattggtg agtaagctgt acggtgccct caaaaggtaa
    23821 tactcccagg ttttccggca agaacggatt tggttccgga agcttcaatg cagaaagttt
    23881 ttttgagtta cgccaatttt taacccattt tggattactg tatgtcagtg agtactcaac
    23941 accgtaatac tcttcttttt cagttgtctt gacatccctc gcagccaaaa tggccagcat
    24001 attttccgga gtcaaactgt aaagcatact gtcaaaaatg cgtggtttga attcagtaat
    24061 aatgtaagga tcaacttcta acttacgcat agggtaatac atcatcagcg tggaaaatac
    24121 attgacaagg gaggtcccct caggcttttc ggcaaacttg taatctatct cggacatcag
    24181 tttcacctct tcataaatgt aacgtggcaa tcccgtttcc cgcaacaggc gcaaatactg
    24241 aaaaacacgc cggatgactt ttgtgtagtt ccggagacct tttggtgtaa gctgaatttt
    24301 aacatcaaaa ctcgaaaatg atttattgga atctcctcct ccggcagaca atccggccgc
    24361 caggttttcc cgttttaaca gcgaaagcaa actgcctttg ccttcatgtc ccagcagaaa
    24421 acccagcata ccgaggggct gacttttgta gtaatgaagt gtacgtgaca atggaaacat
    24481 caatgtcagc gagcgtgaat ctttgatggt tttaatacgc atcaaacgaa aacgggggtc
    24541 gcgcttcata tattttgcag aaacccgaat ttcctgcaag ttacggtttt tcaccgaaac
    24601 aaaacgtgga acgacccatg actgtaaagt gtctaaatcc tggggacctg ccacggccag
    24661 catcatttga ttacttgaat aatgcttttg gtaaaaatac agcagctctt ttctgcttac
    24721 gcctgccagt gttttcagtg ttccggtagc aaaatgcctg gcagggtgac ctttttcaaa
    24781 ggctttacgt tttacctgaa atattctgcg aaagtcattt gggatgtttt tggagtgttc
    24841 agaatcaatc gctttcattt cacgttccac taatccggaa ttaaacagcg gggccagaaa
    24901 aaattgtgag aaacgatcca ggccttcttc aaaataatcc ggatccacct caaagtgata
    24961 gtttgtacgg tcatttgcag tataggcatt actgaaacca tcatgtgttg aaacaaattc
    25021 ctgatagctt ccctcatcag gatatttctc agttcctaaa aaaagcatgt gttccagaaa
    25081 atgtgccagt ccagaacgtc cacttggatc gctcatcgaa cctactgcta cagtcaaaga
    25141 agccgcacct cgctgcaggg taggatcaga tatcaacatt actttcattt gattgggaag
    25201 cagaaagcgc cggtattgac gttgaggata acgagggtca gtattgttca gtcctccgcc
    25261 ccaaactgaa atagaggctc cagctaaaaa acaaattgcc aaaaatagag tcagaatttt
    25321 gcgcattatt aatttctctt gttggttaca gttgaaggtt ttcaccctta atacatttca
    25381 ttttgagaat atttacaaaa aaacgcaatt ggaaagaagg cgtttttgta ttgactatca
    25441 aggcactcct attacgatag tttatactca ttattttttg ttaaatcaac tgaaggtgta
    25501 acccaaatta taagtgtctg aatttgaccg ttgacgtgac attttgcaaa atcggaaatg
    25561 aaggtctcag aaccgcttac aataggactt tcatggacct tatcttcagg tgctgacatt
    25621 gtttcttctg aaatatgaga tcctttgcgg gcattttaaa aaaacgaagg aaaggcatat
    25681 gaatactatc tctcttaatt tagctttgat ttaaacttaa catcaaccag ctgttttaaa
    25741 acaacatcaa ccaactacta atagtaatcc atggaaaata aagaatattt tgtgcgagtt
    25801 tctgaattgt tcgtgagggt cgaggagaag cttgacgaat ttgaggacga aattgactat
    25861 gacccaacac cggataagct aatggtcaca tttgaaaata acagtgaaaa aattgtgatc
    25921 aatacccagc gtgcaatcaa ggaaatctgg ctggccggga attcaagagg ctggcatttt
    25981 cagtttctgc aagaaaaaga tatctggttt gctaatgcag aacaagaaga attctaccaa
    26041 tgtttagcta aattattatc agaaaacctg gggacatcag tttcttttga atagtacgaa
    26101 aaagtgaaag ctttttctat aaacatttaa tccttttttc ggtaattccc ggactttccc
    26161 cctgtttttt ctactaaata aacagactca atctccatac ttttggtaac tgatttacac
    26221 atatcgtata aggtcaaagc tgcaatggaa gcccctgtca gggcttccat ttccactccg
    26281 gtacgtcctg tagttttggc agtggcctga ataaaagcat actcaccctt cagttcaatg
    26341 tccacattta ctccggaaag cggcaaaggg tgacacattg gaatcagttc tgaggtccgc
    26401 tttgctccct gtattcctcc aatgacagct gtttgcagaa ccggaccttt ttttgtactt
    26461 ccggtatctt taagaagtgc cgcgagttct gctcctaaat gcacgcgtgc tactgcaacg
    26521 gctttacgtt cagtcacaga tttttcggaa atatcgacca tccttggttg atcatttacg
    26581 gataggtgtg taaattcatt cttggaatca ggcatgattt tcactggaac aggagagttt
    26641 tatttttact gatttttaaa aggtatccag cgggaatcct tcaaaatttg ggctggctga
    26701 aaatctgcct tgtaattcat tttgtgagaa ctttctaccc aatatcccaa atataaccag
    26761 cgaatatcaa gaaagcgggc atattcaatt tcatacagga gagaaaatat tcccaacctc
    26821 cttgattcaa attccggatc aaaaacaaaa taaactgaac ttattaattc cggtaaacaa
    26881 tccagccacc ccacaccgat gagcttggtt cctaaataat aacgcaggat ttcggttggt
    26941 acaggagttt caatcaaaaa atcaaagtac tcactttcct caaccggata cttgatctcg
    27001 tgccaatcta tctgataacg ctggtaaaga ttaaaatctt cctggttaaa tccaactgga
    27061 tgatgctcaa cacgaacatc cttgtttttt cgccacgtgc ggcgttgccc ttttccttga
    27121 gaaaatttct gtacatcgat ccgaatcggt atgcaggcct ggcaaccgga acaaacggga
    27181 tgataaatgg aaagaccgct ccggcgaaac ccctgattca acagcgaagt gtaaccgtct
    27241 gcaggaatct cactggtctg gaaagagata ttgtgccaag tataattatc tcgataagga
    27301 caaggaccgt tgtctgtctg ataaagcttt atctggcttc cgctcatttt caatcttcag
    27361 taatgaggaa aaaggcgcaa aggactcaaa tcaggacaat cctgtggcaa tcactgtcat
    27421 ggaaacttca tctcctgctt catcagagaa ggatgccccc cagattaact caacgtcttc
    27481 atcagccata tcctcaatga gtgacattgc ttcctgaatt tcataagcac tggtatcttt
    27541 tgggccactc acatgaacca aaattccttt cgctccacgg atgtctgtat cttccattag
    27601 agaaccatgt aatgcatcct ctacagcatt caatgctctg tccttacctt ttgccactcc
    27661 tcgaccaatg actgccttac ccatcccttt cattatggtt tccacatcag caaaatcgac
    27721 attaatgtca ccgactccgc tgagcagaag agtaatccct ctgatagcat tgatcagcac
    27781 catatcaacc atgtgaaaag cttccaggaa gcttgtactt tgagtactgg catcgagaag
    27841 tttgtcgttt ggtatgacca gtaaagtgtc agtgtattca cgcagacgtt caactccggc
    27901 aagccctgag tccaggcgtt ttgtaccttc aaatgaaaaa ggaattgtaa caataccaac
    27961 tgtaagaatt tgcttatctt ttgccagtcg agctattgtc ggagcagcgc cggttccagt
    28021 tccaccgccc attccggcag caatgaaaac catattagca ccttcaacag cttcacttaa
    28081 ttctgcaaga ctctcttccg cagcaccttc accaacttca ggacgtgctc ctgcccccat
    28141 cccacgtgta agttttttgc ctagctctat ctttttatgt gatttgttac gctttaaggc
    28201 ttttacatca gtgttgcaaa taatggtctc aacctgatca agattttctt ctgccatttg
    28261 cgttaccaca ttccctccag caccgccgac accaattatt tttatgtttg ggatgaatgc
    28321 ttcctgaaca ggagaaaatt gcgtagtttg tttttctgtc atggatttca actcaaaaat
    28381 aaattgggga ttaaagttaa attagttacc aatgaagaaa gaatattttc agattctagc
    28441 ttagatatta acacataaaa tgtaataatt gttgactaaa ccttgcattg attcaataat
    28501 gatgatatag ctgtataatt gaatttgcaa accaaagaat tggagatggt taaaaaaccg
    28561 ctttgattcg cttcattgaa aaaaactatt atgctttctg aacaagtatt ttatctcttt
    28621 ttatttccct ggttcttttt tctagcaatt attccaggtg ttgtaatgac attcctcttt
    28681 gcaggcattg cgggacaatt gctgaactat ctcccgggaa aaaatggaga ttaaattaat
    28741 gcaattttaa taagtccaaa ctggataaaa ttcaatacta ataacactaa aaaaaacaat
    28801 aataacaagt ctctttgtcc tctgtttttt ctgttg
//
LOCUS       DTVD01000104            8887 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2116_length_8887_cov_207.138134, whole genome
            shotgun sequence.
ACCESSION   DTVD01000104 DTVD01000000
VERSION     DTVD01000104.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2116_length_8887_cov_207.138134"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="EYO68_03745"
     CDS             complement(<1..824)
                     /locus_tag="EYO68_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HIC06949.1"
                     /translation="MIKDWSSTFEITKKYLRYLFKRFKEDRIIVYSGYLSFVTLLSIV
                     PLLAVIFSVFSLFPVFQDWRGEVESFVFKNFVPTLGESIQGYLIRFVENATNMTSLGL
                     VALFLVALLLISSIDHTLNHIWRVRKNRRQLVSFSIYWVVLTIGPVLIGISLLTTSYL
                     FSLTGFEDNTLLAVRKLFIAFLPYLGSFSSFLLIYLLVPHTRVHFWSALSGAMIASVL
                     FELSKSAFALYFTHFPVYKEIYGALAIIPLLFVWIFISWVVILVGAQVAASLDGFL"
     gene            complement(879..>2119)
                     /locus_tag="EYO68_03750"
     CDS             complement(879..>2119)
                     /locus_tag="EYO68_03750"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIC06950.1"
                     /translation="DGPLIIERGPCENPLFSAFFEAVQEAGYPLTDDVNGYKQEGFAR
                     FDRNIHRGRRMSAARAYLHPVKKRPNLTVQCRTLTTKILFEGKNPESSSRAVGVEFSR
                     SPGRSEKVYAGVIICCGGAINSPQLLQLSGIGNADELKKLGIDVVHDLPGVGENMQDH
                     LEVYIQYACKKPVSMAPALKWWNKPWIGYQWLFHRKGPAATNHFEAGGFIRSNEHEKY
                     PNIMFHFLPLAIRYDGSSPGAGHGYQVHVGPMYSDAQGSVKIQSTDPRVYPKLRFNYL
                     STEQDRREWGEAVRCARNILNQPAFGEFNAGELSPGPQVESDEEVLNWVAKDGETALH
                     PSCTCKMGTDDMSVVDPVSMKVHGVESLLVVDASVMPYITNGNIYAPVMMLAEKAADL
                     ILGNSPLPTEDVEYYRTALE"
     assembly_gap    2120..2219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2220..2632)
                     /locus_tag="EYO68_03755"
     CDS             complement(<2220..2632)
                     /locus_tag="EYO68_03755"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890515.1"
                     /note="catalyzes the oxidation of choline to betaine
                     aldehyde and betain aldehyde to glycine betaine; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIC06951.1"
                     /translation="MPKSIYDTVIIGGGSAGCALANRLSEDPSHKVLVLEAGRPDYIW
                     DIFIHMPAGLTFPIGNKYYDWCYESEPEPFMNNRRIFHGRGKVLGGSSSINGMIFQRG
                     NPMDYQRWAGDAGMKNWDYAHCLPYFKRMENCLAGG"
     gene            complement(2659..3441)
                     /locus_tag="EYO68_03760"
     CDS             complement(2659..3441)
                     /locus_tag="EYO68_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HIC06952.1"
                     /translation="MSQNSILELEFKTDTGLGNRANIGIIVLRTDQTLEHEFANLLNL
                     AGVALYHSRIPNEMEVTKENLANMEEELPVAAALLPALFNFDVIGYGCTSGSTIIGEK
                     NVEKAIQVVHPDVPTSNPLTACKAALQALGVKRVAFLTPYDPGITTTMRDNLLVSGFE
                     IPVTGSFFESDDFVVGRIDPESILEAIEKIGARDDCDGVFVSCTNLRVASIIETAEAR
                     LGKPVTSSNHALAWHLLRLAGIQDCPENFGSLFQVQLNEHDE"
     gene            4239..5096
                     /locus_tag="EYO68_03765"
     CDS             4239..5096
                     /locus_tag="EYO68_03765"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC06953.1"
                     /translation="MLWSTGGPLIRLLEGASEWQFLFYRSIALASALFLLIRIKSPNT
                     ITQFKKAGIASVIGGLFLSLAFVTFVFSVTHTTIANTLFMLSSAPFIAGLFGWILLRE
                     RIDKTQWTAMTAAAIGILLMIQDGLSGDTLFGNLTAFAAAVGFAGFTVSLRWGKNENM
                     LPAVCYAGLFTVFFSAFAAVFLNDGLSISRNDLFIATGFGAFGLGFGMVLYVAGSYKM
                     QAAELVLLSLLEIILGPIWAWMFFSELPTSLTMIGGVILLSAILFQTFSGMEIFQKKL
                     QTVTVKKMQ"
     gene            5108..6661
                     /locus_tag="EYO68_03770"
     CDS             5108..6661
                     /locus_tag="EYO68_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase"
                     /protein_id="HIC06954.1"
                     /translation="MADRKHRGRTGRSGGGRAAKQAERSAPVTKGVPYITRKVPVYEV
                     LDEEGLSLIENNAETILQEIGVEFRGDAEALQMWKTAGADIDGERVRIPKGLCKKLIQ
                     DSAPAEFTQHARNPERNVRIGGKNMVLVPAYGSPFVRDLEKGRRYATLEDFQNFVKLA
                     YSTPYLHHSGGTICEPVDLPVNKRHFDMVYTHMRYSDKPFMGSVTAPERAQDTVDMAK
                     VLFGEEFVQNNTVITSLINANSPLVWDATMIGALKVYALNNQACVVSPFCIAGAMSPV
                     TVAATAAQLYAEALAGMAFAQLVRPGAPVIFGTFSSSMSMQSGAPTFGTPEPQLVLYI
                     VAALARRLGVPFRSGGGLNSGKIADAQAAYEAANTLQHAMLAGVNFMLHTAGWLEGGL
                     SMGYEKFIMDIDQAGMIHEFLNGVDLSENGQALDAIREVGPGKHFLGCDHTRANFETA
                     FFRSSVADNNSFEQWEADGGLDTAQRANKIWKKMLNDYEMPPIDPAVDEALLAFIRQR
                     KESFPDANY"
     gene            6667..8733
                     /locus_tag="EYO68_03775"
     CDS             6667..8733
                     /locus_tag="EYO68_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC06955.1"
                     /translation="MSTADPLLQPFQLKHLRLKNRIISTSHEPAYSEDGLPKERYRLY
                     HEEKAKGGIAMTMFGGSTLVAPDSPPVFGNLYAGNDKIIPFFQEMADGVHKHGAALMC
                     QITHLGRRSVSNAGDWLPIVAPSCVREEVHRGFPKIMEESDIRRIVKAYGAAAKRCQL
                     GGLDGVEIEAYGHLFDAFWMKRTNFRTDRYGGSLENRVRFSLEVLEEIRKQVGNDYIV
                     GIRMVFDEGLDENLAGGLRLEEGLKIGEMLVETGALDFINIIKGHIDTEEGESLVLPS
                     MGIPAAPYLEFAGAIKREFELPVFHAARINEVATARHALRENLLDMVGMTRAHMADPY
                     IVKKIESGEEDHIRPCVGMGYCLDRLYENNDALCAHNPATGREKTMPHFVPKTSGSVR
                     KIVVIGAGPSGLEAARVCAERGHSVVLFEANDRPGGQLLLAAKIERRREIIGIVDWLF
                     TEVQRLGVEVRFNCYAEASEILAEIPDVVIVATGGLPNTEFLREGADLVVPSWDILAG
                     SVKPKNNILLFDDNGQHPGISCAEFLAKRASHPETNLEYVTPERTIAPDVGGTNYPAY
                     LTALYENEVRITLNHRLTEVRRNTSGLVATLYNEYTHSNIERTVEQIVVEHGTLPLDE
                     LYFELREQSSNGGEIDLEALISGNAQNLVNNPEGDFQLFRVGDSVASRNVHSAIYDSL
                     RLCKDL"
BASE COUNT         2492 a         1833 c         2052 g         2410 t
ORIGIN      
        1 tccagaaaac catccagact cgccgctacc tgagcaccta ccagaataac tacccaggaa
       61 ataaagatcc aaacgaaaag cagtggtatt atggccagag caccgtaaat ttccttataa
      121 accgggaaat gagtgaaata aagggcaaag gcggatttgg aaagctcaaa caaaactgag
      181 gcgatcattg ctccgcttag agcactccag aaatgaacgc gggtgtgtgg taccagcaaa
      241 taaatcagca ggaatgacga gaaggatcct agataaggaa gaaacgctat aaacaactta
      301 cggacggcaa gcagggtgtt atcctcaaaa ccggttaaag agaacagata ggaggttgtc
      361 agaagactga tacctatcag aacaggacca atagtcagta ccacccagta tatcgaaaag
      421 gagactagtt ggcgacggtt tttgcgtact cgccaaatgt gattcaatgt gtggtctatc
      481 gaagaaatca gcagcaaggc caccagaaag agggccacaa gacctaacga tgtcatattg
      541 gttgcatttt ccacaaatcg aatgagatag ccctgaatcg attctcccag agtaggcaca
      601 aaattcttga agacaaatga ttccacttca ccccgccaat cctgaaaaac aggaaaaaga
      661 gaaaagacag aaaatatgac cgcaagcagc ggtacgatcg aaagaagggt gacaaatgaa
      721 agatatccgg agtagactat gatcctgtct tctttaaaac gcttgaaaag atatctgaga
      781 tattttttgg ttatttcaaa tgttgaagac cagtctttaa tcatttgcta atctgttctt
      841 gtattataag ttgcatttcc ggactatttt aagagaagct attccaatgc tgtacgataa
      901 tactcaacat cttcagtcgg aagcggagaa ttccccaaaa tcaagtctgc ggctttttca
      961 gccagcatca tcaccggcgc ataaatgttt ccgttagtta tgtagggcat cacggaagcg
     1021 tcaaccacta gcaggctttc cactccgtga accttcatac ttaccggatc cacaactgac
     1081 atgtcatctg tccccatttt acaggtgcaa gatggatgca gcgcggtttc tccatctttg
     1141 gcaacccaat tcaatacttc ttcgtcactc tcaacttgcg gcccgggtga caactctccg
     1201 gcattgaatt caccaaaagc cggttgattc aaaatattgc gtgcgcaacg caccgcttca
     1261 ccccactccc gacggtcctg ctcggtggac aaataattaa agcgcagttt gggatagact
     1321 ctagggtcag tggactgaat tttaactgag ccttgcgcat cggaatacat tggtccaaca
     1381 tggacctggt aaccatgtcc tgctccggga gatgatccat cataacgtat agcaagaggt
     1441 aaaaagtgaa acatgatgtt tgggtatttt tcgtgttcgt tactgcggat gaaacctcct
     1501 gcctcaaaat gattggtggc ggccggacct ttgcggtgaa aaagccattg ataaccgatc
     1561 cagggtttgt tccaccattt taaagccgga gccatggata caggtttttt acaggcatat
     1621 tgaatataaa cctctaaatg atcctgcata ttttccccca ctcccggtaa atcatgaaca
     1681 acatcaattc caagtttttt cagttcatca gcatttccga ttccggaaag ctgcaaaagc
     1741 tgcggtgagt ttattgcccc tccacaacaa atgatcactc cagcatatac tttttctgaa
     1801 cgtcccggac tcctggaaaa ctctactcca acagcacgtg atgaagactc ggggtttttc
     1861 ccttcaaata gaattttggt ggtcagtgtc cggcactgaa ccgtgagatt cggacgcttt
     1921 ttaaccggat gcagatatgc ccgtgccgca ctcattcgcc gtccgcgatg tatgttccgg
     1981 tcaaaacgtg caaaaccttc ctgtttgtaa ccgttaacat cgtccgttaa tggataacct
     2041 gcttcctgta cggcttcaaa aaatgcactg aacaaaggat tttcgcaagg gcctctttct
     2101 atgatcaacg ggccgtcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     2221 cgcctgccaa acaattttcc atacgtttga aatagggcag acagtgtgcg taatcccagt
     2281 ttttcatgcc ggcgtcgccg gcccagcgct ggtagtccat cggatttccg cgctggaaaa
     2341 tcataccgtt aatactgctt gaaccaccca gaactttacc acgcccatgg aaaatgcgcc
     2401 tgttgttcat gaaaggttcc ggttctgatt cataacacca atcgtagtat ttgttgccga
     2461 taggaaatgt taagcctgca ggcatgtgga taaaaatgtc ccagatataa tccggccggc
     2521 ctgcttccag tactaagact ttgtgtgaag gatcctcgct caaacggtta gccaatgcac
     2581 aaccggcgga accaccgcca atgatgactg tatcgtaaat actttttggc atgcagtaac
     2641 tttatttagc ttggttgatt attcatcatg ttcatttaac tgcacctgga aaagactgcc
     2701 aaagttttcc ggacaatcct gaataccagc taaacggagt aaatgccagg ccagagcgtg
     2761 attgcttgag gtgacaggtt tacccagacg tgcttctgcc gtttcgataa ttgatgctac
     2821 ccgcaaattt gtgcaagaaa caaatacacc atcacaatca tcgcgagcac caatcttttc
     2881 aattgcctca agaattgatt ctgggtcaat acgaccgaca acaaaatcgt cagattcaaa
     2941 aaaagatcct gttactggaa tttcaaagcc ggaaactaga agattatctc gcattgtagt
     3001 agtaattcct ggatcgtatg gagtaagaaa agcaacgcgc ttgactccaa gtgcttgtaa
     3061 agcagcttta caggcagtca agggatttga ggtaggtaca tcgggatgta caacttggat
     3121 cgccttttca acattttttt caccaataat agttgaacca gaagtacagc catagccgat
     3181 tacatcaaaa ttgaataagg caggaagcaa agcagcagca accggtaatt cttcttccat
     3241 gttagccagg ttttccttcg ttacttccat ttcgttggga atgcgtgaat gataaagcgc
     3301 gacacctgcc aaatttaata aattagcaaa ttcatgttcc agagtttgat ctgttcgaag
     3361 cacaataata ccgatatttg cccggtttcc caggcctgta tcagtcttaa actctaattc
     3421 taaaatacta ttttgactca tcttggaaga tccacttagg tgtaaacaaa taaaacgtag
     3481 cctttttaga acaattcaaa gacaaatatg aaatctaaga acttaggtga ggatgtaaca
     3541 gggatactaa caatcatttt tctcaagcac aaggcggaac caatcaagtt aataaacgaa
     3601 tacatatgac ggtctcgcaa taagtctgaa atcaacactg gaatttcgat catagggagg
     3661 aatcttcatg atattagttt agtagtttgt tcaggatttc tcgcttcgtt ctaaatgaca
     3721 cggtttttgg tctttagagg acggcgtcat atattaacag aaataacgca ataatccggg
     3781 atcttttatt aagaatgtct ggtcatcagc attaaatcgg aactcttaat tcttatattt
     3841 tacatttagg aactcatagt tccgtttttg tagatcagca aagatgaaga tctagtttaa
     3901 tcaattagta tttatgtata taatattatt agttatttaa ttacaaattc ttgtatttgc
     3961 acgataatac tttttagatg cctattttta gccaatactt cacttaattt agcagaactg
     4021 taagttcctg tgttttaata taatataatt ttatattgta ttttcaatgt tttatagttt
     4081 ggcatcgggt atgcaaaata gacagatcgt gttgattcag aataccagat aaagattact
     4141 ataaaataaa cgaaacattt ccttcaatat tgaaataggc aattttgttt acaacaactt
     4201 ctattacaca gggccgatca tttgtcatta tcgcaggtat gctatggagt accggaggcc
     4261 cgctgatacg attgttggag ggtgcttctg agtggcaatt tttattttac cgctcgattg
     4321 cattagcatc tgctctgttt ttactgataa gaattaaaag tccaaatacc ataactcagt
     4381 tcaaaaaagc cgggatcgcg agtgtaattg gaggactgtt tctctcttta gcttttgtta
     4441 cgtttgtttt ttcagtcact catacaacga ttgcaaacac tctttttatg ttgagttccg
     4501 cccctttcat tgccggatta tttgggtgga ttttattaag agagaggatt gataaaactc
     4561 aatggacagc catgacagca gccgctattg gcatcctgct catgatccag gatggattgt
     4621 cgggagatac actttttggt aatttaactg cttttgcagc tgcagtaggt tttgcgggtt
     4681 ttaccgtttc gctgcgctgg ggaaaaaatg aaaatatgtt gcccgctgtt tgttatgcag
     4741 gactgtttac ggtatttttc agtgcgtttg cagcagtgtt tttaaacgat ggactttcta
     4801 tctcgcgtaa cgatttattt atagccacag gatttggagc atttgggctg ggcttcggaa
     4861 tggttttgta tgttgctgga tcatacaaaa tgcaagcggc agaattggtt ttactcagtt
     4921 tgttagaaat tattttgggg ccgatttggg cttggatgtt ttttagtgaa ttaccgacat
     4981 cgttgaccat gattggaggc gtaattttgc tctcagcgat tctctttcag actttttcag
     5041 gaatggaaat atttcaaaaa aaacttcaaa ctgtgacagt caaaaaaatg caataattgg
     5101 ataaatcatg gcagatcgaa aacatagagg tcgtaccgga cgttccggcg gtggaagagc
     5161 agcaaaacag gccgagcgaa gtgcaccggt gacgaaagga gttccgtaca ttacccgcaa
     5221 agttccagtt tatgaggtat tggatgagga aggattgtcc ttgattgaaa acaatgcgga
     5281 aactattttg caggaaattg gtgttgaatt ccgaggcgac gctgaagctt tgcaaatgtg
     5341 gaaaacagcc ggagcggata ttgacggtga gcgtgttcgg attcccaaag gattgtgcaa
     5401 aaaattgatt caggacagtg caccagcaga atttacgcaa cacgccagaa atccggaaag
     5461 aaacgtccgg atcggtggaa aaaacatggt tttggtcccg gcttacggtt caccgtttgt
     5521 acgggattta gagaaaggaa ggcgttatgc cacgcttgaa gattttcaga atttcgtaaa
     5581 acttgcttat tcgactccgt atttgcacca ttccggagga acgatttgcg aaccggtgga
     5641 tttgcctgtc aacaaacggc attttgacat ggtttacacg cacatgcgtt acagcgacaa
     5701 accgttcatg ggttcggtca ctgcaccgga acgggcgcaa gatacagtcg atatggcaaa
     5761 ggttttattt ggtgaagaat ttgtacaaaa taacacggtc atcaccagtt tgatcaacgc
     5821 taattcaccg ttggtttggg acgcaaccat gatcggcgca ttgaaagttt atgccctaaa
     5881 taatcaggct tgcgtggttt ctccattttg cattgccgga gccatgtcgc ccgttacggt
     5941 ggcggcaaca gcggcgcagc tttatgcaga agcacttgcc ggaatggctt tcgcacagtt
     6001 ggttcgtccg ggtgcgccag tgattttcgg cacattttcc agttcgatgt cgatgcagtc
     6061 cggtgcaccg actttcggta ctccggaacc gcaattagtg ctttatattg tagctgcttt
     6121 ggcgcggcgt ttgggtgttc ctttccgaag cggcggcggt ttaaatagtg gaaaaatcgc
     6181 tgacgcgcaa gcggcttatg aagcggccaa cactttgcag cacgccatgc tcgccggagt
     6241 gaatttcatg ctccacaccg caggttggct tgaaggagga ttgtcgatgg gttacgaaaa
     6301 attcatcatg gacattgatc aagccggaat gattcacgaa tttctcaatg gcgttgatct
     6361 ttccgagaac ggtcaggcat tggatgcgat tcgcgaagtc ggccccggaa aacacttttt
     6421 gggatgcgac cacacacgag ccaattttga aacagcgttt ttccgttcca gcgttgccga
     6481 caacaacagc tttgaacaat gggaagccga cggcggtttg gatacggcac aacgagccaa
     6541 taaaatttgg aaaaaaatgc tcaatgatta tgaaatgccg cccatcgatc ccgcagtcga
     6601 tgaagcactt ttggccttta tccgtcaaag aaaagaatca tttccggatg caaattattg
     6661 agaataatga gtacagctga tccacttttg cagccgtttc aactcaagca tttacgcttg
     6721 aaaaatcgga ttataagcac cagccacgaa cctgcttatt ccgaagacgg attgcccaaa
     6781 gaacgctacc ggctctatca cgaggaaaaa gccaaaggcg gaattgcgat gacgatgttc
     6841 ggtggttcaa ctttggttgc tccggattcg cctccggtgt ttgggaattt gtatgcagga
     6901 aatgacaaaa tcattccgtt ctttcaagaa atggcggacg gcgtacacaa acacggtgcg
     6961 gcattgatgt gtcaaatcac acacttgggg cgtagaagtg tttccaatgc cggggactgg
     7021 ctgccgattg tcgctccgtc ttgtgtgcgg gaagaggtgc atcgcggttt tccgaaaatt
     7081 atggaggaat ccgatattcg tcgcattgtt aaagcctacg gcgcggcggc aaaacgctgt
     7141 caacttggcg gattggacgg tgtggaaatc gaggcttatg ggcatttgtt cgatgctttt
     7201 tggatgaaaa gaacaaattt ccggacagac cgttacggcg gtagtttgga aaatcgagtt
     7261 cgattttctt tggaagtttt ggaggaaatc cgtaaacaag tcggcaacga ttatattgtc
     7321 ggaatccgga tggtctttga cgagggcttg gatgaaaact tagcaggcgg tttgcgtttg
     7381 gaagaaggtc taaaaattgg tgaaatgttg gttgaaaccg gtgcgttgga ttttatcaat
     7441 atcatcaaag gtcacatcga cacagaagaa ggagagtcac tggttttgcc gagcatgggc
     7501 ataccggcgg ctccgtattt ggaatttgcc ggagccataa aacgtgaatt tgaattgccg
     7561 gtttttcatg ccgcccgcat taacgaagta gcaacggcac gacacgcgtt gcgggaaaat
     7621 ttgcttgaca tggtcggcat gacacgggcg cacatggcag atccgtacat tgttaaaaaa
     7681 attgaatccg gagaagaaga ccacatccgg ccttgtgtcg gcatgggtta ttgtttggat
     7741 cgtttgtacg aaaataatga tgcactttgc gcccacaatc ctgcgaccgg acgcgaaaaa
     7801 actatgccgc attttgtgcc gaaaacttcc ggaagtgtgc ggaaaattgt cgtaatcggc
     7861 gccgggcctt ccggactgga agccgctcgt gtttgtgcag aacggggaca ttcagtcgtt
     7921 ttatttgaag caaatgatcg acctggcgga caattgcttt tggctgcaaa aatagagcgg
     7981 cgacgggaaa tcatcggaat tgtcgattgg ctttttacgg aagtgcaacg gctcggtgtt
     8041 gaggtacgtt tcaattgtta cgccgaagct tccgaaattt tggccgaaat tccggacgtg
     8101 gtgattgtgg caaccggagg tttgccgaac acagaatttt tgcgggaggg cgcagatttg
     8161 gtggttccga gttgggacat tttggcggga agtgtgaaac cgaaaaataa cattttgctc
     8221 tttgacgaca acggccagca tcccggaatt tcctgtgctg aatttttggc aaagcgagcc
     8281 tcacatccgg aaacaaatct tgaatatgtg actccggaac gcaccattgc gccggatgtc
     8341 ggcggcacaa attatccggc gtatttgact gcgctttatg aaaatgaggt aaggattaca
     8401 ctcaatcatc gtttaacgga agtgcgccgt aacacttccg gattggtcgc cacactttac
     8461 aacgaataca cgcattccaa cattgaacgc acagtcgagc aaatcgttgt cgagcacgga
     8521 actttaccgc tggatgaact ttattttgaa ctgcgtgaac aatcgagcaa tggtggtgaa
     8581 attgatttgg aagccttgat ttccggaaat gcgcaaaatt tggtgaataa tccggaaggg
     8641 gattttcagc tatttcgggt tggtgattcg gttgccagcc ggaatgttca ttcagccatt
     8701 tatgattctt tgcgcttgtg caaggatttg taaaaggttt gccaaaaact cacaaaaact
     8761 cacaaaaaca aaagatagat ttttgtgagt ttttgtgagt ttttggcgaa aaatataatt
     8821 tcgcaattta atcaacctca aggagaatat tatgagcgaa gaacaagact taattgaaga
     8881 cgaagaa
//
LOCUS       DTVD01000105            2227 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_21213_length_2227_cov_245.800184, whole genome
            shotgun sequence.
ACCESSION   DTVD01000105 DTVD01000000
VERSION     DTVD01000105.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21213_length_2227_cov_245.800184"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="EYO68_03780"
     CDS             complement(<1..761)
                     /locus_tag="EYO68_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIC06956.1"
                     /translation="MKIKDLKTFVVGNPAPHFGGRYFIFLKLITGDGIEGVGEVYCAT
                     FSPHIIVKMIEDVFERHVEGSNPFRIEALWRNVYGRGYTLRPDVSLMGVLSGLEIALW
                     DICGKSVDKPVFELLGGKVNEKLRSYTYLYPKDGAVYTEDEPHVYNNPELAAEAAAEY
                     VAQGFTAIKFDPAGAYSVFDPRQPSLETMDRSELFCKRIREAVGSKADLLFGTHGQFT
                     ASGAVRLARRLEAYDPLWFEEPTPPERPEEMAKVAR"
     gene            complement(771..1886)
                     /locus_tag="EYO68_03785"
     CDS             complement(771..1886)
                     /locus_tag="EYO68_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIC06957.1"
                     /translation="MNSHNLKIHSIETFSNEYVGLVRVRTKDGSEGWGQVSPYNADIT
                     ALLVHRQIAPYALGADALDIEKLVQEIPEREHKFPGSYLRRALAGLDTALWDLRGKLE
                     NKSVCELLGGTPRPLRVYASSMKRDITPQAEAERFLRLRDEFGYDAFKFRVGRECGHD
                     EDEWPGRTEEIVPEIRKALGVDVALLVDGNSAYTPEKAISVGRLLEDNGICHFEEPCP
                     YWELEWTKQVTDALKLDVTGGEQDCDLSTWKRIIEMRAVDVVQPDVCYLGGLTRTLKV
                     AEMARQAGIPCTPHSANLSLVTVFTLHLMGALENAGPYVEFSIEGPEYYPWQYGIYEP
                     ALAAKDGKVQIPEAPGWGIEINPDFLEKTKYQISTLN"
     gene            complement(1942..>2227)
                     /locus_tag="EYO68_03790"
     CDS             complement(1942..>2227)
                     /locus_tag="EYO68_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIC06958.1"
                     /translation="ERVGDKPLYITFDLDCLDTTVAPGVANLEPAIEGFMMPDVLKLL
                     QGMRGRNIIGGDVVCLMPTKDAPNQITAHVANAILFEMICLIADNFRGSS"
BASE COUNT          575 a          581 c          478 g          593 t
ORIGIN      
        1 cgggcaactt ttgccatttc ttccggacgc tccggaggag tcggttcctc aaaccaaagc
       61 ggatcgtagg cttccagacg acgtgccagc cgaacagcac cggaagcagt gaattgtccg
      121 tgtgtgccga acaacaaatc tgctttggaa ccgacggcct cgcgaattcg tttacaaaat
      181 aattcggaac gatccatagt ctccaatgaa ggttgacgcg gatcaaaaac cgaatatgct
      241 cctgccggat cgaacttgat tgcggtgaag ccttgcgcca catattccgc cgcggcttct
      301 gcggcaagct caggattgtt gtaaacatgc ggttcgtcct cggtataaac tgctccgtct
      361 tttggataaa gataagtgta agaacgtaat ttttcgttaa ctttaccacc cagtaattca
      421 aaaaccggct tgtctacaga ttttccacaa atgtcccaaa gcgcaatttc caaaccgctc
      481 aagactccca tcagtgaaac atccggacgt aaggtgtagc cgcgtccata tacattgcgc
      541 caaagtgctt caatccggaa aggattgctt ccttctacat gacgctcaaa aacatcttca
      601 atcattttta cgataatgtg tggactaaaa gttgcgcaat aaacttcgcc gacaccttca
      661 ataccgtcgc cggtaatgag tttgagaaaa atgaaatatc gtccaccaaa atgcggcgct
      721 ggatttccga ccacaaatgt ttttaagtct ttgattttca caaaatattc ctaattcaat
      781 gtactaatct gatacttggt tttttccaga aaatccggat tgatttcgat tccccatccc
      841 ggagcttccg gaatttgcac tttaccgtct tttgcagcca atgcgggttc gtagatgcca
      901 tattgccatg gataatattc tggaccttca atggaaaatt cgacatacgg cccggcgttt
      961 tccaaagctc ccattaagtg gagtgtgaaa acagtgacca gggagaggtt ggctgagtga
     1021 ggtgtgcagg ggattccggc ttgacgagcc atttctgcga ccttgagggt gcgtgtcagt
     1081 ccgccaaggt agcacacatc cggttgcact acatcaacag cccgcatctc gattattcgt
     1141 ttccaggtgg ataaatcaca atcctgctca cctccagtca catccagctt gagtgcatcc
     1201 gtgacctgtt ttgtccactc cagttcccag tatggacacg gctcctcaaa gtgacagatt
     1261 ccattgtctt ctaaaagtcg accaactgaa atcgcttttt ccggagtgta ggcactgttt
     1321 ccgtcaacga gcaacgcaac atctactcca agtgctttgc ggatttccgg aacgatttct
     1381 tcggtacgtc ctggccactc atcctcatca tgtccgcatt ccctgccaac acggaacttg
     1441 aaagcatcat aaccgaattc gtctctcaaa cgcaaaaacc gttctgcttc ggcttgcgga
     1501 gttatgtcac gtttcatact ggaagcataa acccttaacg gtcttggtgt tccgcccagc
     1561 agttcgcaaa cgcttttgtt ttccagtttt ccgcgcaaat cccagagtgc ggtatcgagt
     1621 ccggcgagtg cacggcgcaa atatgaaccc gggaatttgt gttcacgttc cggaatttcc
     1681 tgaaccagtt tttcaatgtc cagggcatcc gcacccagtg catacggcgc aatctggcga
     1741 tgcaccaaca aagccgtaat atccgcattg taaggtgaaa cctgccccca gccctcggaa
     1801 ccatcttttg tacgtacacg taccaacccg acatattcat tgctgaaggt ctcgatgcta
     1861 tggattttca aattgtgtga attcatggaa taaaataaat tctgaagact gttttcattg
     1921 cattttgcat ttcaccatac ctcaagagga accgcgaaaa ttatccgcaa tcaaacagat
     1981 catttcgaag aggatggcat tggcaacatg cgcagtaatt tggtttggcg catccttggt
     2041 cggcatcagg cagaccacat ctcctccaat gatatttctg ccacgcatcc cctgcagcag
     2101 tttcaataca tccggcatca tgaaaccttc aattgccggt tcaaggttag ctactcccgg
     2161 agcaactgtt gtatccaggc agtcaagatc aaaggtaatg tacagtggtt tatctcctac
     2221 tcgttcg
//
LOCUS       DTVD01000106            8880 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2121_length_8880_cov_324.962493, whole genome
            shotgun sequence.
ACCESSION   DTVD01000106 DTVD01000000
VERSION     DTVD01000106.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_8880_cov_324.962493"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="EYO68_03795"
     CDS             <1..555
                     /locus_tag="EYO68_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017429410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIC06959.1"
                     /translation="VGVVLSLILSWYNGSLNRRSFLDALMGATRTSCMIAFILAGAAF
                     LTVAMGFTGIPRVLAQWVGSLGLSPNMLLVTLTIFFVVLGCFLEGISIVVLTTAVIQP
                     IIEQAGIDRIWFGIFIVIVVEMAQITPPVGFNLFVIQGLTGHNILQVARATIPFFFLL
                     LAAVIIISIFPEIVTYLPEQMGRR"
     gene            715..4584
                     /locus_tag="EYO68_03800"
     CDS             715..4584
                     /locus_tag="EYO68_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase"
                     /protein_id="HIC06960.1"
                     /translation="MAIPSFRFSIDRGGTFTDVYAEVPGEPGFRVVKLLSEDPQNYPD
                     APREGIRRILESVTGKIFPKESFPAENIEWIRMGTTVATNALLERKGAKTVLVTTKGF
                     RDLLQIGNQSRPKIFDLEIRKLDLLYEEVIEVDERVRIIKDSEKSDPDSGLEIAEGTT
                     GEKFAVLAKPDLESVSKELEAVFEKGILAVAVVFLHAYAFPEHEREIGKIADEIGFEQ
                     ISLSHEVMPMVKMVARGDTTTVDAYLTPHIRNYLESFRSGFSDNLEKSQLLFMQSDGG
                     LTDSGNFKGSNAILSGPAGGVVGYAVTTELGQPVIGFDMGGTSTDVSRYGDDYELVHE
                     TETAGVRIQAPQMHIKTVAAGGGSRLFFRNGLFEVGPESAGAHPGPVCYRKDGFLAVT
                     DANLVLGRLHPEYFPKIFGSNENEALDFESARKAFEKITAEINAYSKERKLPAMSLEE
                     VALGFLRVANEVMVRPIREISVMRGFDIKEHALACFGGAGGQHACAIARDLGISKIFI
                     HRFSGILSAYGMGLADIVVEKQEPSALVLPENKEDNSLNYLLKNLNKLAAEAKTELLA
                     QGYKSEQIEIKRYLNLRYQGTDTALMIPEPDSTSKYDTGITNCELGISESSPELQKNA
                     EKFPDYVSAFRETYLREFGFDLTGREIIVDDLRVRAVAKSPGLQKFPLAKKSGTPEVE
                     DRTRCYFMAESSGSGRWQETPIFRLENLGTGQRLQGPVIVMQNTSTILVEPGCTAEIT
                     EYGDVLLNVETKTFREIGTQADPVQVSIFGNLFMSIAEQMGRTLQRTAISTNIKERLD
                     FSCAIFDETGGLVANAPHQPVHLGAMSEAVRQQVKLQGDNLKEGDVLLTNHPIAGGSH
                     LPDITVISPVWQGEKPIFYIASRGHHADIGGISPGSMPPFSRELKEEGACIKTFKVVE
                     NGIFNEAGITELLLAPGKIERNPGEPAISGTRLLSDNLSDLKAQVAANQRGIDLLLEM
                     IEHYSLEVVQAYMHHIQNNAEEAVRNMLKTLSVREDLAEVDILEANDFLDDGSEIVLH
                     LTIDRRDGSAIFDFTGTGPELWGNLNAPRAVTHSAILYSLRCLIDQEIPLNQGCLNPI
                     KVIIEDGSLLAPSENAAVVGGNVLTSQRITDVILKAFRACAASQGCMNNFTFGNENFG
                     YYETIGGGAGAGPNWHGQSGVHTHMTNTRITDPEILERRYPVMLREFSIRKDSGGSGK
                     FKGGNGLIREVEFLEPLNTAILSERRVHQPYGLNGGGPGKSGLNLFIRKDGSKLHLGG
                     KNEIIAEAGDRIRIETPGGGGYGKVGT"
     gene            complement(4585..4902)
                     /locus_tag="EYO68_03805"
     CDS             complement(4585..4902)
                     /locus_tag="EYO68_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014123658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HIC06961.1"
                     /translation="MREPVLLGELAKLIRSKNAGPFWITFDIMFANDTDFKRVVSAKV
                     LTKSWIAQTYQVEEDSVIFVEITAASAIKFSFPRSRIQGDPGETDMYSGQQYAPLLNI
                     EIR"
     gene            complement(4902..6272)
                     /locus_tag="EYO68_03810"
     CDS             complement(4902..6272)
                     /locus_tag="EYO68_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIC06962.1"
                     /translation="MTASRISLISPTGALGMGFLDQSLERGISLKPDVIACDAGSTDS
                     GPFYLGSGTPKMSRRAVLRDLRRLLLARDQLKVPLIIGSCGTSGVDSGVDWMREMTLE
                     IAREEGLSFKLGRIYSEQKPESMAQAFQSGNIEALPGAPEIDEQLIQNCSHIVAMMGH
                     EPIVSLLKKQCDVILCGRASDTALFSALPLMRGFLPGPVWHCAKTIECGAICSTSTRA
                     DGVFAEIDDNGFSVEPLALDASCTPLSLASHTLYENADPYLIREPSGMLNTQNARYQK
                     LSERKTRVEGSVFRPDRYTLKLEGATCTGFQTVAIGGVRDPYIIARVDSWLAEMKVFF
                     AERLKELTGKTLGKEVRLDISQYGKNAVMGELEKSSAQIPNEIGLLFCVTAPEQALAN
                     DVARFITHTASHWPIPEWDGFISGIAFPFSPPEIDRGPVYRFVLNHVLIPESPLSAFR
                     FEMENI"
     gene            complement(6282..7784)
                     /locus_tag="EYO68_03815"
     CDS             complement(6282..7784)
                     /locus_tag="EYO68_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIC06963.1"
                     /translation="MLDGMLLGLETAFTFQNLFFAALGCFIGTIIGMLPGLGPMSVVA
                     IMIPVSLQIGDPSTTLILLAGVYYGAIFGGSTSSILINAPGEAAVVATAFDGYPMARK
                     GQAGKALTIAAISSFSGGTIGVVLLMFFAPALAGFAILFWSAEYFALMLLGLSAVSAF
                     AGKGKVLKAVMMTLLGLMLATVGESSLFHAPRFTLGIMDLQSGINFVTLAMGLFAVPE
                     AFFLAIDKIRSKKSSSKKSQEISNLRINLKEAKAIAPVIGRQSIQGFLIGVMPGTGAT
                     IASFLGYAVERNLASPEEREEFGKGSIKGLAAPETANNAASTGSFVPLLTLGIPGSGT
                     TAVLLGAFIALNLQPGPQLLQERPEVFWSVIMSMYFGNIVLLVLNLPLVPYIARLLAI
                     PRGYLIPFVLFFAMAGAYIGQNNSTELLLLLGFGVLAIILRFADYPLPPLLIGFVLGQ
                     MFEDNLGRAMRNANGIEFIWERPMTLTLILTAAVFILVPILRDWLANKRK"
     gene            complement(7873..8394)
                     /locus_tag="EYO68_03820"
     CDS             complement(7873..8394)
                     /locus_tag="EYO68_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIC06964.1"
                     /translation="MSLLFRSDMLDLNRAVALALLGFSSGYGYLAYFYHLLPFEKFLA
                     VRPNTMPIGLAIAGIICSLVILIQPQVPEVEGDGTVNRPDAEYLQNPQNYNWGQGIGL
                     LLLAVVYALSLRGAGFLFCTSAFLVLGGILLGERRYTFLIPISITASVMVWYLVDQVL
                     TVYLRPWPGWVYY"
     gene            complement(8443..>8880)
                     /locus_tag="EYO68_03825"
     CDS             complement(8443..>8880)
                     /locus_tag="EYO68_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIC06965.1"
                     /translation="PTKVKYIPFDAGGKAMAALLSGEIDALSTGFSEAVSMEKAGKVR
                     MIAVTSSERVDAAPNAPTLIEQGYNVTFVNWRGFFAAPDLPSNMRKAYIEVLGKMYKT
                     SEWESVRARNGWVNIYNPGDKFMVFLAEQEKVIGDLMRELGFL"
BASE COUNT         2448 a         2016 c         2138 g         2278 t
ORIGIN      
        1 gtcggcgtgg tactttcgct gattctttca tggtacaatg gcagcttgaa tcgacgaagc
       61 tttctggatg cattgatggg tgcaacccgg acctcatgca tgattgcctt tattctggct
      121 ggagccgcgt tcttgacagt agccatgggt tttaccggta ttccgcgggt actggctcag
      181 tgggttggtt cattgggatt gagtcctaat atgctgctcg taaccctgac catttttttc
      241 gtagtcctgg gttgttttct ggagggtata tcgattgtgg ttttgaccac agcagtgatc
      301 caaccaatca tcgagcaagc cgggatcgac cgtatctggt tcggcatctt tattgtcatc
      361 gttgttgaaa tggcgcaaat tacgccaccg gtgggattca atctattcgt gatacaaggt
      421 ttgacaggcc ataatatttt gcaggttgca cgcgccacta taccattctt tttcctgtta
      481 ttggccgcag tcataattat ttctatcttt ccggaaatcg ttacctacct accagaacag
      541 atggggaggc gctgaagcga aagtcgtaga gagataacgt aagttttcat ttcaattcat
      601 ttgaatgatc tttaccacgg catagttgac cgtctttccg gaaattgcac tctttctgcc
      661 tggcttgatg gtcaagtaat ttcacttacg taacaggttt tgtttaacta aatcatggct
      721 attccttctt ttcgtttttc aattgaccgc ggaggaacat tcaccgatgt ttacgcggaa
      781 gttcccggtg aacccggatt ccgtgtggtt aaattacttt ccgaagatcc gcagaattat
      841 ccggatgcac cgcgcgaggg aatacgccga attcttgaat ccgtgacggg aaagattttt
      901 ccaaaagaaa gttttcctgc agaaaacata gagtggattc gtatgggaac cactgtggcc
      961 acgaatgcac ttttggagcg taaaggtgcg aaaacggtgc tggttaccac aaaaggtttt
     1021 cgggatttgc tgcaaatagg aaatcaaagc cggcctaaaa tttttgatct ggaaatcaga
     1081 aaacttgatt tgctttatga agaagtgatt gaagtcgatg agcgtgtccg gattattaag
     1141 gactcagaaa agagtgatcc tgattcagga ttggagattg ccgaaggcac aaccggggag
     1201 aaatttgctg tattggcaaa accggatttg gagtctgtca gtaaagagtt ggaagcggtt
     1261 tttgaaaaag gcatactggc ggtggcagtg gtttttttgc acgcttacgc atttccggaa
     1321 catgaaaggg aaatcggtaa aattgccgat gaaatcggct ttgagcaaat ttcattgtcg
     1381 cacgaggtta tgccgatggt aaaaatggtg gcacgtggtg acaccactac cgttgatgct
     1441 tacctcacgc cgcacatccg gaattacctg gaaagtttcc gttccggatt ttccgataat
     1501 ctggaaaaat cgcagttgct tttcatgcag tccgacggag gtttgacgga ttccggaaac
     1561 tttaagggca gtaacgccat tttgtccgga cccgcaggag gtgttgtagg ctatgccgtg
     1621 accaccgaat tggggcaacc ggtaatcggt tttgacatgg gcggaacctc gacggatgtt
     1681 tcccgttacg gtgacgatta cgaattggtg catgaaacgg aaaccgcagg ggtacgcatt
     1741 caagcgccgc aaatgcacat taaaacggtc gcggccggtg gtggatctcg gttgtttttt
     1801 cgcaacggtt tgtttgaagt tggtccggaa tccgcgggtg cgcatccagg accggtatgt
     1861 tatcggaagg acggatttct ggcggtcact gatgccaact tggtgctggg acggctgcat
     1921 ccggaatact ttccaaaaat tttcgggtca aacgaaaatg aagctcttga tttcgaatcc
     1981 gcacgaaaag cctttgaaaa aataactgcc gaaatcaatg cttattccaa agagcggaaa
     2041 ctaccggcaa tgtccctcga agaagtcgcc ctcggttttt tgcgtgtcgc caatgaagtc
     2101 atggtgcgtc ccatccgtga aatttctgtg atgcgggggt tcgatatcaa ggaacacgct
     2161 ttggcctgtt tcggaggtgc cggaggacag cacgcttgcg ccattgccag agatttaggc
     2221 atttccaaaa ttttcatcca ccgtttttcc ggcattcttt cagcatacgg catggggctc
     2281 gccgacattg tagttgagaa acaggaacct tcagcgctgg tgctgccgga aaataaagag
     2341 gataacagcc ttaattatct cttaaaaaat ctcaacaaat tagccgctga agcaaaaaca
     2401 gaattgcttg cacaaggtta caaatctgaa caaattgaaa tcaagcgtta tcttaatttg
     2461 cgttatcagg gtactgacac tgctttgatg attccggaac cggattcgac ttcaaagtat
     2521 gacacaggca ttacaaactg tgagttaggc atttcagagt caagtccgga attacagaaa
     2581 aacgcagaga aatttccgga ttatgtaagt gccttccggg aaacgtatct gcgtgaattc
     2641 ggttttgatt tgacgggacg tgagatcatt gtggatgatt tgcgtgtacg tgcggtcgca
     2701 aaatctccgg gattacaaaa atttccgctt gcgaaaaaaa gcggaactcc ggaagtagaa
     2761 gaccgaacac gttgttattt catggccgaa agttcgggtt ccggcagatg gcaggaaaca
     2821 ccgatttttc ggctcgaaaa tttgggaacc ggacagcgtt tgcagggtcc ggtgattgtg
     2881 atgcagaata cgtcaacgat tcttgtagaa cccggatgta ccgctgaaat tacggaatac
     2941 ggtgatgtgt tattgaacgt tgaaaccaaa actttcaggg aaatcggaac tcaagccgat
     3001 ccggttcagg tgtccatttt cggtaacctt tttatgtcga ttgcagagca gatgggacgt
     3061 accctacaac gtaccgcaat ttccaccaac atcaaagagc gtctcgattt ttcctgtgcc
     3121 atttttgatg aaactggagg tttggtggcc aatgctcctc atcagccggt gcatcttgga
     3181 gcaatgagtg aggccgttcg gcagcaagtt aaactgcagg gagataatct caaggaaggc
     3241 gatgttttac tgaccaacca tccaattgcc ggaggaagtc atcttccgga catcaccgtc
     3301 atttcgccgg tttggcaagg cgaaaagccg atattttaca ttgctagccg cggccatcac
     3361 gctgatattg gcggaatttc accgggctcg atgccgccgt tttcgcgtga attgaaagaa
     3421 gaaggtgcgt gtattaaaac tttcaaagtg gtggaaaacg gaattttcaa cgaagccgga
     3481 atcaccgaac tgctgttggc tcccggaaaa atagagcgca atcccggaga accggcgatc
     3541 agcggcaccc gtttactttc ggataatctt tccgatctca aagcacaagt cgcagccaat
     3601 caacggggta tcgatttatt gctggaaatg atcgaacatt attcgctgga agtggttcag
     3661 gcctacatgc atcatatcca aaacaacgcg gaagaagcgg tgcggaatat gctcaaaacg
     3721 ctttccgtac gggaagattt ggccgaagtt gacattttgg aagcaaatga ttttctggat
     3781 gacggcagcg aaatagtttt gcatctcact atagatcgaa gagacggcag tgcgattttc
     3841 gattttaccg gaacaggacc tgagctttgg ggcaacctga atgcaccaag agcagtaact
     3901 cattcagcga ttttgtacag tttgcgctgt ttgatagatc aggaaattcc gctcaatcaa
     3961 ggctgtctca accccatcaa ggtgattatt gaagacggtt cattgctggc tccatctgaa
     4021 aatgctgctg tggtcggagg taatgttttg acttctcagc gaatcacgga tgtaattctc
     4081 aaagcattcc gtgcctgcgc ggcttcgcag ggctgcatga acaatttcac cttcggcaac
     4141 gaaaatttcg gctattacga gacgattggc ggcggcgctg gtgcaggccc aaactggcac
     4201 ggacaatccg gtgttcacac tcacatgacc aacacacgca taaccgatcc ggaaattctg
     4261 gaaaggcgtt atccggtgat gcttcgtgaa ttttctatcc gcaaagattc aggtggttcc
     4321 ggaaaattca aaggaggaaa cggactcatt cgtgaggtcg aatttctgga accactcaat
     4381 actgccattc tttcagaacg ccgtgtccat cagccttatg gactaaacgg cggtggaccc
     4441 ggaaaaagcg gattgaacct ttttattcgc aaagatggtt ccaaactcca cttaggcggt
     4501 aaaaatgaaa ttattgctga agcaggtgac cgaatccgca ttgaaactcc gggaggaggg
     4561 gggtatggga aagtgggtac ctgattatct gatctcaata ttcagcagcg gcgcatactg
     4621 ctgtccggag tacatatctg tttcgccggg atcaccctga attcttgaac gtggaaacga
     4681 aaatttgata gcactggcag cagtgatttc aacgaaaatt actgaatctt cctcaacctg
     4741 ataagtttgg gcaatccagc tttttgtcag tacttttgca gatactactc gcttaaaatc
     4801 cgtgtcattt gcaaacatga tgtcgaaggt tatccagaac ggccccgcgt tttttgagcg
     4861 aatcaatttt gctaattctc ccagaaggac tggctctctc atcagatgtt ctccatttca
     4921 aaacggaaag cggataatgg tgattccgga attaaaacat gattcagcac gaaacgatag
     4981 actggtcccc ggtcgatttc cggtggagaa aaagggaaag caattccgct gatgaaacca
     5041 tcccattcgg gaatgggcca gtgtgaagca gtatgggtaa tgaatctggc aacgtcattt
     5101 gccaaggcct gctcaggtgc agtaacgcag aataagagtc caatttcgtt aggaatttgg
     5161 gcagaacttt tttccagttc acccatcact gcatttttac catattgaga tatgtcgagc
     5221 cgtacctctt ttccaagtgt ttttcctgtc aattctttaa gtcgttctgc aaaaaatact
     5281 ttcatttctg ccagccagga atccactctg gctataatat aagggtcacg cactccacct
     5341 atggcaacgg tttggaagcc tgtgcaggtg gctccttcga gtttgagggt ataacgatca
     5401 ggccgaaata ctgaaccctc aacacgtgtt ttgcgttccg aaagtttttg gtagcgtgca
     5461 ttttgtgtat taagcattcc ggaaggctcg cgaatcaggt aaggatctgc gttttcataa
     5521 agcgtgtgcg aagctaaact gagaggtgtg caggacgcgt caagagccaa aggttcaacg
     5581 ctgaatccgt tatcgtctat ctccgcgaat actccatccg ctcgtgtgct ggtggaacaa
     5641 atcgctccgc attcgatcgt tttcgcacaa tgccagaccg gccccggaag gaaaccacgc
     5701 atcaacggaa gtgcagagaa aagtgctgtg tcagaagcgc gcccgcagag aatcacatca
     5761 cattgttttt tcaacaaact cacaatcggt tcatgtccca tcatggctac gatatgtgag
     5821 caattttgaa ttaattgctc atcgatttcc ggcgctcccg gaagtgcctc aatatttccg
     5881 gactgaaacg cctgtgccat cgattccggt ttctgctccg aataaatcct gccaagttta
     5941 aaactcaaac cttcttcacg agcaatttca agcgtcattt cccgcatcca atccactccg
     6001 gaatcaactc ctgatgtccc acaggagcct atgatcaacg gaaccttcaa ttgatcacgc
     6061 gctaataaca aacgtcgtaa gtcccttaat actgcacgac gtgacatttt tggagttccg
     6121 gaaccgagat aaaaaggacc ggaatcagtt gaccctgcat cgcaggcaat cacatcgggt
     6181 tttagtgaaa ttccgcgttc cagtgattga tccagaaaac ccatgcccaa tgcaccagta
     6241 ggactgatca gagaaatacg ggaagctgtc atacacaatt gttacttgcg tttgtttgcc
     6301 agccaatccc tgagaatcgg gacaaggatg aagactgctg cagtaagaat cagggttaga
     6361 gtcattggac gttcccagat aaattctatt ccgtttgcat tacgcattgc acgtccaaga
     6421 ttgtcctcga acatttgacc cagcacgaaa ccaatcagca gcggaggcaa gggataatct
     6481 gcaaatctca agataatggc caagacacca aaacccagca gtaaaagtag ttctgttgaa
     6541 ttgttctgac caatgtacgc tcctgccatg gcgaaaaaca ggacaaaggg aatcagatag
     6601 ccacgtggaa ttgccagcag tctggcaatg tatggaacca gcggcagatt aagtactaga
     6661 aggactatgt taccgaagta cattgacatg atcacagacc agaaaacttc cggccgctcc
     6721 tgcaagagtt gtggtcctgg ctgcagattc agggcgataa aagctcccaa cagaacagca
     6781 gtcgttccgg aaccgggaat tccaagagtc agcaacggca cgaaggaacc tgtactggcc
     6841 gcattgtttg cagtttctgg tgcggctaaa cctttgatgg agcctttacc gaattcttca
     6901 cgttcttccg gactggcaag gttcctctct actgcataac cgaggaacga ggcaattgta
     6961 gcgcctgttc cgggcattac tccaatcagg aatccttgaa ttgactgcct tccgattacc
     7021 ggtgcaatgg ctttggcttc tttgaggtta atgcgaaggt ttgatatttc ctgggatttt
     7081 ttggaactgc tttttttact gcgaattttg tctattgcca gaaaaaaggc ttccggaact
     7141 gcaaacaaac ccattgcaag agtgacaaag ttaattccac tctgcaaatc cattatgccc
     7201 agggtaaatc taggtgcatg gaaaagcgac gactccccaa ctgtagccaa catcagtccc
     7261 agcagtgtca tcatgactgc tttgagtact ttacctttgc ctgcaaaagc ggaaactgca
     7321 gacaaaccga ggagcatcaa agcaaaatat tctgcagacc aaaataatat cgcaaagccg
     7381 gcaagtgccg gagcaaaaaa cattaacaac accacaccaa tggttcctcc ggaaaaagag
     7441 gaaattgccg caattgttaa ggccttgcct gcctgcccct ttcgggccat gggatagccg
     7501 tcaaaagccg tggctaccac tgcagcctcg ccaggagcat taatcaggat tgatgaggtg
     7561 gaacctccaa aaattgcacc gtaatagact ccggccagca aaatcagtgt tgttgaggga
     7621 tcaccgatct gaagacttac cggaatcatg attgccacca ccgacatcgg tccgagtccg
     7681 gggagcatcc cgattatcgt accgatgaaa cagcctaagg cagcaaaaaa caagttctga
     7741 aaagtaaagg ccgtttccag ccccagcaac attccatcaa gcattggatt tctccagtac
     7801 aaattgctta aggtgaggca gtgacctgaa aatttttgaa aggctttgaa tatgaatcat
     7861 ataattgcta gatcaatagt aaacccaacc tggccagggt cgtaaataaa ctgtcagaac
     7921 ttgatcgaca agataccaaa ccattacact tgcggtaatt gaaatcggaa tgagaaaagt
     7981 gtagcgccgc tcacccagta aaattccacc taaaaccaga aaagcggagg tacagaaaag
     8041 aaagcctgct ccacgcagag acagagcata aactacggca aggagcaaga gtccaatgcc
     8101 ttgaccccag ttataatttt gagggttttg aagatattct gcatcaggtc gattgacagt
     8161 tccatcacct tcaacttctg gaacttgtgg ctggataaga ataacaagag aacaaatgat
     8221 ccccgcaatt gccaaaccaa tcggcatggt gtttggtctt acagccagga atttttcaaa
     8281 aggcaggaga tggtagaaat aggcgaggta gccgtaacca ctggaaaaac ccagcaatgc
     8341 taaagccact gcacggttta gatcaagcat gtcagaacgg aataaaagac tcacagttcc
     8401 gggaagtcgg aaaacttccc ggaaattatg aattagaatg gtctacaaga atcccagctc
     8461 acgcatcaga tcaccgataa ctttttcctg ttctgcaagg aaaaccatga acttgtcacc
     8521 tggattataa atgttgaccc atccatttcg ggcacgcact gattcccact cggaagtttt
     8581 gtacatttta ccgagaacct caatgtaggc ttttcgcata ttacttggca ggtctggagc
     8641 agcaaaaaat cctctccagt tgacaaaagt tacattgtaa ccttgttcaa ttaacgttgg
     8701 agcatttggt gccgcatcaa cgcgttcaga tgaagtaaca gctatcattc gaactttccc
     8761 agctttttcc atgctgactg cttccgaaaa tccggttgaa agggcatcga tttctccgga
     8821 aagcagggcg gccatggcct tccctccagc atcaaaagga atgtatttta cttttgtcgg
//
LOCUS       DTVD01000107            8781 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2154_length_8781_cov_342.558904, whole genome
            shotgun sequence.
ACCESSION   DTVD01000107 DTVD01000000
VERSION     DTVD01000107.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2154_length_8781_cov_342.558904"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            360..1262
                     /gene="prmC"
                     /locus_tag="EYO68_03830"
     CDS             360..1262
                     /gene="prmC"
                     /locus_tag="EYO68_03830"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIC06966.1"
                     /translation="MTIEQWTIERILNWTQGHFEKVRPDSPRLDAELLLAYVLDCPRL
                     DLYLRADQPLNEKERGAYRELVQQRAAGCPIAYLTGEKDFWSLTLEVSKATLIPRPDT
                     ETLVETSVEKILKWQQTHPEATCNIAELGTGTAAIPLALCAELQNLNIISVDCSAETL
                     TVAARNLERYQNLLAPRNNSLKLVESDLFSAIEPAANLDFIISNPPYIPTETISTLQV
                     DVCQYEPSIALDGGPDGLFFYKYLLKTGPSLLNKSGEMILEIGFDQQVELTELQDDFP
                     EWTSSTFLPDLQGNIRVCCLQLEC"
     gene            complement(1259..2506)
                     /locus_tag="EYO68_03835"
     CDS             complement(1259..2506)
                     /locus_tag="EYO68_03835"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC06967.1"
                     /translation="MEVLILGGGVVGVTTAYQLLKDGHQVLVIDRLPPGIGGASYGNA
                     GLIATGHSIAWGSPKALKIWFNSLFHQDPVFRMKFQADPQFIRWGLKFLFQCTEYKAK
                     RNSLAKHRISSYSQKKLKEVVEETNIQYERNANGLIYLYRNSQAMAASSSHVRLLQEI
                     GQTLETVEKERALEIIPELKDSKDQIAGGVFSPTDESGDSKIFTEQLMEFCRGMGGTF
                     ESGVAIERLEASGTKIKRVVTDRGVFTADNYVLCLGAWSPLLVRTSLGVRISVCPVKG
                     YSITIPVEKGHTPPRIGGLHEEDLLGFAPMGNHFRVSSVSEFTGYDIGHTPEDFEHIL
                     STSKKLFPNAGNYDKAEFWSGFRPMTPEGTPILGKERHSNLFYNTGHGHLGWTMSCGT
                     ARITADLIAGKTPEISTELMGVR"
     gene            complement(2533..3420)
                     /locus_tag="EYO68_03840"
     CDS             complement(2533..3420)
                     /locus_tag="EYO68_03840"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC06968.1"
                     /translation="MSDQFKGLSFALLATLGGGLYAIPYRLSLETVAPLTVVWGVFLW
                     ALLFSLPGAWLARHQTKYSWKIGRITLLISLAGLLGNYSICQALNRASPTMLVLLSRS
                     EIIIAMLLSWLFLKEIITARVWLAVVVIIAGIIVMKLNTLSFELNEWSAIIWAITAAS
                     SFAVMQVLAKSIIHEINPQVLNVSRLALALVILWSLEEVRSRVAVLEFTEWQWLALAA
                     FCGPFVGRVSYTYALRYLTISKTVIIGSFSPVATLIFELLVFGTLINGYEAFGGTIML
                     AGILWVFLPGLRRSVKLVK"
     gene            3901..5538
                     /locus_tag="EYO68_03845"
     CDS             3901..5538
                     /locus_tag="EYO68_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06969.1"
                     /translation="MSKESADITERIVKLKPDWVLFSASAFETPELCLNLLQEVQNIS
                     RKNLRFVLAIDEINPGLTILLKLQPVFELVNKMQFKISDPDLLLTHHIRSFPRIRLEK
                     DFRTLDYTDNSGTLVRQSPSEVPLNTLIPFKNIQKIETRKAGTAPEKWLNNFLLERDS
                     VAHPDQVVGILRETKGCYLFPGIPFNSILSLKIDKTKIEHVIRLDECSIKNPPFKRFI
                     ENMEQEHRLWLSADKERAKRASVHIHCSGKYPIINTLMQKLLKEIGYNNFKLISEIKN
                     EELKQKNSDIYLKLNNFPANKIRQKHIDWSKDLNQILEPLNHFIFLSDLKMENISAAL
                     PIHKIEFEEFRDNLLKEIKDAETKNQQAQSDQMLHTQERNILKKITPFSRKLLEVLSA
                     SRTWESAVELASKIKQPRAILFCENENVAAELNLTLTEVPRKLWINPFKFQHAEDLTQ
                     LNSKMTHSYLKPGTIIISASARTHLENLCRKALLESKQAETVLHEQKLHIKKIKANLE
                     LLQNKKNKSAFRWLHVSLKQLLYRDRHLFQIPQGKTE"
     gene            5535..6461
                     /gene="glyQ"
                     /locus_tag="EYO68_03850"
     CDS             5535..6461
                     /gene="glyQ"
                     /locus_tag="EYO68_03850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIC06970.1"
                     /translation="MNSSHTIQEIILLLNEFWNKQGCILMNPYDVETGAGTMNPMTTL
                     RTIGPEEWNVAYVEPSRRPADGRYGENPNRLYQHHQYQVILKPSPDNVEELYLASLEA
                     LGINPLDHDIRFVEDNWEAPTLGAWGLGWEIWLDGMEVTQFTYFQQVGGIECQPVSAE
                     LTYGIERIASYLQDVEDVFDLEYTKGVSYASIFRQPEFEHSKYTFEVADTELMIRWFN
                     DYEQEAGRALAENLVFPAYDYVLKCSHTFNQLDAAGAISVSARASYIGRVRTLAQKIA
                     KLFLSERCKLAFPLMKDREAAQKWVEILTPEN"
     gene            6477..8600
                     /locus_tag="EYO68_03855"
     CDS             6477..8600
                     /locus_tag="EYO68_03855"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:PRK01233.1-1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIC06971.1"
                     /translation="MNTYLLEIGLEEMPAQMILPAVEQLKSLANKTCEVYQLSFDNIR
                     TFSTPRRLTVQLQGLPEKQADRKIELKGPPAAIAKDAKNNWSKAAQGFAGKNGVSVDN
                     LEIREFEGKEYLFVQREELGKPVPELLQTEAEQWISQLNFPKNMRWGSYRMRFIRPIR
                     WIVSLWNDEVVPVKLEMVEAGNETRGHRFLSSGKSVLKQAAEYEKLLEKLNVMADFEK
                     RRESILSQVKKLEQKHGFEVELEDRLLTEVTNLVEWPTVLLGDFEKDFLELPSEVLVT
                     SMAVHQRYFPVFQKNEDNKTGEKKLLPNFVTVRNGDERALETVRRGNAKVLRARLSDA
                     RFFYLNDQKKALSDFQTKADNVVFFQQRGSQAQRVQRIAELSVYIAHALNLSKAQKKQ
                     VQRIAELSKFDLGTRMVAEFPELQGVMGENYAKLKNEPALVCSGIREHYYPRTAKDSL
                     PQNLETVAVAVADKLDMLNTAFSLDMIPTGAADPFALRRTAQGIIQLILGSGISLGLH
                     DLTSEAIRLLDAQQKLGLDRSKLQQELIEFLLQRQRWYMQQRNIRYDLIEAVLQFRPS
                     ERAESKALPVEQLQLAEFLGKHLETDIFKRSVEAIVRAANISYKYPKKIAKNMDQSAL
                     KLTEEKNFFTALQPILNSDQQARWNPENYLKQLHAIEPVVTRFFEDVMVMDEDPVKCG
                     NRLWLCSQLAEWSGRHLDLRAIVFA"
BASE COUNT         2718 a         1837 c         1805 g         2421 t
ORIGIN      
        1 agaatattac tggatgcaca ccaatctctg aaaaacggca gctgaaattt tattggtgaa
       61 gcagactaaa tgtgtctgaa cgaaaatctg tccaacccag ttattttcag ggtttcagca
      121 ggttttaaat aaccaaagat ttagccgtca gttttctgct gtcagatccg tatacgattc
      181 ttctccgttg tcattccggg cggaacaaag agtagacccg gaatccagga gtgtgattta
      241 taatcttatg caaaatccga tcgtgtatct ggatccccga acaagtccgg gatgacaaga
      301 gacaaacatt ttcaataagc ccaaaataat cactaataat taagtagtaa gcaaaagatg
      361 tgactattga gcaatggaca atagaacgaa ttttgaattg gacacagggc cattttgaaa
      421 aagtacgtcc ggactctcca cgcctggatg ctgaactgct acttgcctat gtgcttgact
      481 gtccccgttt ggatctttat cttcgggcag accaaccttt gaacgagaaa gaacgtggtg
      541 cgtaccggga actggttcag caacgtgctg caggttgtcc tattgcatat ttgacaggag
      601 aaaaggattt ctggtccttg acacttgaag taagcaaagc aaccctaatt ccacgtccgg
      661 atacagaaac actggtagaa acttcagtag agaaaatcct gaaatggcaa caaacccatc
      721 ccgaagcaac ttgcaatatt gctgaactcg gaacaggaac agccgcaata cctctggctc
      781 tttgtgctga actccaaaac ctgaatatta tatctgtaga ctgttctgca gagacattaa
      841 cagttgcagc ccgaaattta gagcgttatc aaaatttgct tgctccacgt aacaacagtc
      901 tgaagcttgt agagagtgac ttattttctg caattgaacc tgctgcaaat cttgatttta
      961 taatcagcaa cccgccttac attcccactg aaaccatttc gactttgcag gttgatgttt
     1021 gtcaatatga acccagtatt gcgttggacg gtggtcctga cggcttgttt ttttacaagt
     1081 atctgctcaa aactggtcca agtctgctta ataaaagtgg tgaaatgatt ttggaaatag
     1141 gctttgacca gcaagttgaa ttgacagaac tgcaggacga tttcccagaa tggacttcct
     1201 ctactttcct tccggatctc cagggaaata tccgcgtctg ctgtttgcaa ctggagtgtt
     1261 agcggacccc catcaattca gttgatattt caggtgtttt tccggcaatc aaatccgcag
     1321 taatacgggc cgtaccgcag gacatggtcc agcccaaatg cccgtgtccg gtattgtaaa
     1381 ataaatttga atgtcgttct tttcccagaa taggtgtacc ttccggtgtc ataggtcgaa
     1441 atccggacca gaattctgct ttgtcataat ttcctgcatt tggaaaaagt ttttttgaag
     1501 tactcagaat gtgttcaaaa tcttctggag tatgtcctat gtcgtatccg gtaaattctg
     1561 aaacagaact aaccctgaaa tgatttccca ttggtgcaaa ccccaataaa tcttcttcat
     1621 gcagtcctcc aatcctgggt ggagtatgac ccttttctac tggaattgta atcgagtacc
     1681 ccttgaccgg acagacagat atccgaactc ccagggaagt acgtaccagc aatggactcc
     1741 aggcaccaag acaaagtaca taattatctg cagtgaaaac gccgcggtcg gttactaccc
     1801 gtttgatttt ggtcccggat gcttcaagac gctcaattgc aactccggac tcaaaagtac
     1861 cgcccattcc cctgcagaac tccatcaatt gttctgtaaa aattttggag tctccggatt
     1921 catctgtagg agaaaacact ccacccgcaa tctgatcttt tgaatccttc aactccggaa
     1981 taatctccag tgcgcgttct ttttcaacgg tctccagtgt ctgtccaatc tcctgcagta
     2041 aacgaacgtg tgaactactt gcagccattg cttgcgaatt tcgataaaga taaatcaggc
     2101 catttgcatt tcgttcatat tggatgtttg tttcctctac aacttctttc aatttttttt
     2161 gagaatatga agaaattcta tgttttgcca aagaattcct tttggctttg tattcagtac
     2221 attgaaacag aaatttcaaa ccccagcgaa taaattgcgg atctgcctga aacttcatcc
     2281 ggaaaaccgg atcttgatga aacagggagt taaaccatat tttgagagct ttgggcgagc
     2341 cccaggcaat ggaatgtccg gtggcaatca gtcctgcatt accataactc gctcctccaa
     2401 ttcccggtgg tagacggtca ataaccaaca cttgatggcc gtctttaagt agttgatagg
     2461 cggtagtgac tccaacaaca ccgccgccaa gaattaaaac ttccattttg tttagactga
     2521 tttattttag tctcatttta ctaatttaac acttctgcga agtcccggca gaaaaaccca
     2581 gagaattcct gccagcatta ttgttccgcc aaatgcctca taaccattga ttagagtacc
     2641 aaaaaccaac aactcaaata tcagcgtagc aaccggtgaa aaagaaccaa taataactgt
     2701 cttggaaata gtgaggtaac gcaaagcata agtataggaa acccgcccta caaacggtcc
     2761 gcaaaaagct gccagtgcca gccattgcca ctcagtaaat tctaaaaccg caactctgct
     2821 tcttacttcc tccaggctcc aaaggataac aagtgcaaga gctaatcgtg aaacatttaa
     2881 aacctgtgga tttatttcat gaataatact ttttgccaga acctgcatta ctgcaaaact
     2941 agaagctgcc gtaattgccc agatgattgc agaccactca ttaagttcaa aactcaaagt
     3001 attcagtttc atcacgatta tgcctgcaat aataaccact actgcaagcc aaactcgagc
     3061 agttataatc tctttcaaaa ataaccaact cagtagcatc gcaataataa tctcactgcg
     3121 cgacagtaaa accagcatag tcggggaagc ccgattcaag gcctggcaaa ttgaataatt
     3181 gccgagtagt cctgccaggg agatcagcaa agtaattctc ccaattttcc aggaatattt
     3241 agtttgatga cgtgcaagcc aggctcccgg tagggaaaac aaaagagccc acaggaaaac
     3301 accccagacg acagttagag gtgcaactgt ttccaggctc aaacggtatg gaattgcata
     3361 aagtcctcct ccaagcgttg cgagcaacgc aaagctaagc cccttgaatt gatcactcac
     3421 tgactacttt cttacattca ataatggaca ctatgaaata atattcaggt ttttagacaa
     3481 atcatgcaaa aacataacag catgaatcag gtgctaaggc gacgaggtaa aagttttttc
     3541 ttcaaacatc agtaactcaa aatctcagtg ccttagttta atgtccaata aagtcacaga
     3601 gccagtacag tttgctcaaa aacaatttgc tgctaaatat tgatttttat tgattgttca
     3661 ttttctattt tagggcttaa ataagtcaag taaatttaca tcgttatgaa agcatggtat
     3721 gataagattt tcttttttgc ccctcaaact gtctgattat tctgcacgat gcaacttgtc
     3781 ggcattggtt ttgccagatc atactggatt tcattaatta agcggctcca aaatcaggtt
     3841 tctcatcaat tgaataccga attagttgga gagtccaatt cagttttaaa acctggaatc
     3901 ttgtcaaaag aatctgcgga tattactgaa aggattgtaa agctgaagcc agactgggtt
     3961 ttgttttcag caagtgcgtt tgaaactccg gaactgtgtc taaatttgct acaggaggtt
     4021 caaaatattt ccaggaaaaa tttacgcttt gtattggcaa ttgacgaaat caatcctggt
     4081 ttaacgattt tattaaaact ccagccagtc tttgagttgg tcaacaaaat gcaatttaag
     4141 atttctgatc ctgatttgct actgacacat cacatccgga gttttcccag aattcgtctg
     4201 gagaaagact ttcggacttt ggattacact gataactccg gaacacttgt acgacaatct
     4261 ccaagtgaag ttccgctcaa cactctcatt ccatttaaaa acattcagaa aattgaaacg
     4321 cgaaaagctg gaactgcacc agaaaagtgg ctcaataatt ttttacttga gcgggacagt
     4381 gtagctcatc cggaccaggt tgtgggaatt ctgcgtgaga caaaaggctg ctaccttttt
     4441 ccaggcattc catttaattc catcctgagt ctgaaaattg acaaaactaa gatcgaacac
     4501 gtaattcggc ttgatgagtg cagcattaaa aatccgccct ttaaacgttt cattgaaaac
     4561 atggaacagg aacatcgttt atggttaagt gctgacaaag agagagccaa acgtgcatcg
     4621 gttcacattc attgctcagg caagtatcct attatcaaca ccttgatgca aaagttatta
     4681 aaggaaatcg gatataataa ttttaagtta atttcagaaa ttaaaaacga agaactgaaa
     4741 cagaaaaatt ccgatattta tttgaaatta aacaattttc cagctaacaa aatccggcag
     4801 aaacacatag actggagtaa agatttgaat caaatacttg aaccgcttaa tcattttatt
     4861 tttttaagtg atctaaaaat ggaaaatatt tctgctgctt taccaatcca taaaattgaa
     4921 ttcgaagagt ttagggataa tttattgaaa gagataaaag atgcagaaac gaaaaatcaa
     4981 caagcccaaa gtgatcagat gctccatact caggagcgga atatcttgaa aaaaataaca
     5041 cctttttcca gaaagctgct ggaagtcctg tctgccagca ggacgtggga aagtgctgtt
     5101 gagcttgcat caaaaattaa acagccaaga gcgatcttgt tttgtgaaaa tgaaaatgtt
     5161 gctgctgaat tgaatctcac cttaactgaa gttcctagaa aactctggat caaccccttc
     5221 aagtttcagc atgccgaaga tttgactcaa ctcaattcaa aaatgactca ttcctatcta
     5281 aaaccgggaa ccattattat ttcagcatcc gcacgaactc acctggaaaa cctgtgtcgg
     5341 aaagctcttc tggaaagcaa acaagcagaa acagttttgc atgaacaaaa actgcacatt
     5401 aagaaaatta aagccaattt agagttgctc caaaacaaaa aaaataaatc ggcttttcgc
     5461 tggctccatg tttcacttaa acaacttcta tatcgagatc gtcacttgtt tcaaatccct
     5521 caaggtaaaa cagaatgaac agttcacaca ctattcagga gataattttg ctcctgaacg
     5581 aattttggaa taagcagggt tgtatcctga tgaatccata tgatgtagaa accggagctg
     5641 gaacaatgaa tccgatgacc actctccgta ctattggtcc ggaagaatgg aatgtggcat
     5701 acgtcgaacc ttcgcgacgg cccgcagatg gacgttatgg tgaaaatcca aaccgtcttt
     5761 atcagcatca tcaatatcag gtaattttga aaccatcacc tgataatgtg gaagaattat
     5821 atctggcctc cctggaagca ttggggatca atccgctgga tcacgatatt cgttttgttg
     5881 aagacaactg ggaagcacca acactgggag cctggggact gggttgggaa atttggctgg
     5941 acggcatgga agttacacaa tttacatatt ttcagcaggt aggcggaatt gagtgccagc
     6001 cggtttctgc agaattgact tatggaatcg aaagaatcgc ttcttatctg caggatgttg
     6061 aagacgtttt tgacctcgaa tatacgaaag gagtcagtta cgcttcaatt ttccggcaac
     6121 cggaattcga acattcaaaa tatacttttg aagttgctga cacagaactg atgatccgct
     6181 ggttcaacga ttatgaacag gaagcagggc gtgccctggc tgaaaatctt gtttttcctg
     6241 cttatgatta tgttctaaaa tgctcgcaca cttttaacca gctggatgct gccggagcaa
     6301 tcagcgtttc agcacgtgct tcatacatag gacgtgtacg aacactagcc caaaaaatcg
     6361 ccaaactttt tctgtcagaa cgctgcaaac tggcctttcc cttaatgaaa gatcgtgaag
     6421 ccgctcagaa atgggtggaa atactaaccc ctgaaaatta atgtgatagc tgcattgtga
     6481 atacatattt acttgaaatt ggacttgaag aaatgccggc acagatgatt ctgcctgcag
     6541 ttgaacaact aaaatcttta gcaaacaaaa cttgcgaagt gtatcagttg agttttgata
     6601 atattcgaac tttttccaca ccaagaagat taacagtaca actgcaggga cttccggaaa
     6661 aacaagcaga tcgaaaaata gaattaaagg gtcctccggc tgcaattgca aaagatgcaa
     6721 aaaataactg gagcaaagct gcgcaggggt ttgcaggtaa aaatggggtt tccgttgaca
     6781 atctcgaaat tcgtgaattc gagggcaaag agtatttgtt tgtacaacgc gaagagttgg
     6841 ggaagccggt tccggagtta ctgcagacag aggcagagca gtggatctct caacttaatt
     6901 ttccaaaaaa tatgcgctgg ggttcatatc ggatgcgttt cattcgtccg atacgctgga
     6961 tcgtttcact ttggaatgat gaagttgtac ccgttaaact ggaaatggta gaagccggaa
     7021 atgaaaccag gggacatcga tttttgagtt caggaaaaag cgtacttaaa caggcagctg
     7081 aatatgaaaa gctgctggaa aaactcaatg tgatggcaga ttttgaaaaa cggcgtgaat
     7141 ctattctttc acaagttaaa aagctggagc agaaacacgg ttttgaagtc gagttggagg
     7201 atagactgct gacagaagtt acgaacctgg tagagtggcc cactgttctt ttaggtgatt
     7261 ttgaaaagga tttcctggaa ctgccaagtg aagtgcttgt aaccagcatg gcggtccatc
     7321 agcgatattt tccggttttc cagaaaaatg aagataataa aacaggagaa aaaaagttgc
     7381 ttcctaattt cgtaacagta cgcaacggag acgaaagggc tcttgaaact gtacggcgcg
     7441 gaaatgcaaa agttctgcgt gcacgactga gtgatgcacg tttcttttat ttgaatgacc
     7501 aaaaaaaagc actgtctgat tttcaaacaa aagcagataa tgtcgtattt tttcagcagc
     7561 gtggctcgca ggctcaacgt gttcagcgta ttgcagaact cagtgtttat atcgcacatg
     7621 ctttaaatct ttcaaaagct cagaaaaaac aggttcagcg aattgcggaa ctgagcaagt
     7681 ttgatcttgg aacgcggatg gttgctgagt ttcctgaatt gcagggagtg atgggtgaaa
     7741 attatgctaa attgaaaaat gaacctgctt tggtttgcag cggaatccgg gaacactatt
     7801 atccgcgcac tgcaaaagat tcactgccgc aaaacctgga aacagttgct gttgctgttg
     7861 cggataaact tgatatgctg aacaccgctt tctcgttgga catgattcca accggtgctg
     7921 cagatccgtt tgcattaagg agaacagcac agggaatcat ccagcttatt ttgggatccg
     7981 gtatttcact tggtctgcat gatcttactt cagaagcaat tcgtctactt gatgcccagc
     8041 agaaattggg acttgaccgc tcaaaactac agcaggaatt aattgaattt cttcttcagc
     8101 gccaacgctg gtatatgcag caacgtaaca tccgctatga tttaattgag gcagttctcc
     8161 agttccggcc gtcagagaga gcagaaagca aagctttacc agttgagcag cttcaattag
     8221 ctgagttttt ggggaaacat cttgaaacag atattttcaa aagatccgtt gaagcaatcg
     8281 ttcgtgctgc aaatatcagt tacaaatatc caaagaaaat tgccaaaaat atggatcaat
     8341 ctgctttgaa actgactgaa gaaaaaaact ttttcactgc tctgcagccc attctcaact
     8401 ctgatcagca agcccgctgg aatccggaaa actacttgaa acaactgcat gcgattgaac
     8461 cggtcgtaac ccgttttttt gaagatgtta tggtcatgga cgaagatccg gtgaaatgcg
     8521 gaaatcggct ttggctgtgc agtcaactgg cagaatggtc cggacggcat cttgatttgc
     8581 gggccattgt ttttgcctga aactatttta gccctaagaa cactaagaaa aaaactctct
     8641 aaaactatga aactcttttt actcagtgcc ttctttgcct ttataaatac tttggcactt
     8701 tgaaactctg aaacttttgt aactatgtgc cttagtgcct tagtgcctta gtgccttagt
     8761 gccttagtgc cttagtgcct t
//
LOCUS       DTVD01000108            8749 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2163_length_8749_cov_332.423625, whole genome
            shotgun sequence.
ACCESSION   DTVD01000108 DTVD01000000
VERSION     DTVD01000108.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_8749_cov_332.423625"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="EYO68_03860"
     CDS             <1..462
                     /locus_tag="EYO68_03860"
                     /inference="COORDINATES: protein motif:HMM:PF13478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06972.1"
                     /translation="QLLIIGAGHITIPLVRFAMDLDFLTVVIDPRTVFANPERFPVPP
                     DQLISKWPDDVLPDFPLNEDTYAILLTHDPKIDDPALHILLKQNLPYIGALGSSRTHA
                     KRCARLKEAGFDAASIAQIKGPAGADIGAQTAAEIALSMITEVVAAKHGKL"
     gene            complement(512..2005)
                     /gene="glpK"
                     /locus_tag="EYO68_03865"
     CDS             complement(512..2005)
                     /gene="glpK"
                     /locus_tag="EYO68_03865"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIC06973.1"
                     /translation="MSQIIMALDQGTTSSRTILFDENGKIVAEANAPLVCHYPQSGWV
                     EQVPEEIWSSQRQTIEAALAQAKLSMKDISAVGITNQRESTIAWNRKTGDAIGPAINW
                     QCRRTADFCEELKAEGFDRILKNRTGLVTDPYFSGTKMRWILENVPQARKLADQGNLC
                     FGTVDSWLIWKLTGGQVHATEISNASRTLVFNIDACSWDDEILARFGIPRETLPEVKP
                     SSGVFAQVNSGLFGGEAPIAGVAGDQQAALFGQACFEPGMAKNTYGTGCFMISNTGSE
                     LVFSKHGLLTTIAWQIGNEVTYALEGSVFIAGALIQWLRDGLQLFEDAAETQAMAESV
                     SDSGGVFVVPAFVGLGAPHWDPYARGTIVGLTRDTNRNHIVRASLEAIAYQSAEVLSS
                     IAKDTGTEVKELRIDGGAAANNFLCQFQADLLGLKVTRPQVLETTAMGAAFLAGLAVG
                     VWKDQAQIRSLWQEEKTFTPQLSKNEAVEHMKNWNRAVERSKGWIIP"
     gene            complement(2018..2497)
                     /locus_tag="EYO68_03870"
     CDS             complement(2018..2497)
                     /locus_tag="EYO68_03870"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIC06974.1"
                     /translation="MYRTGIGFDVHTLAEDRLLIIGGVQIPHPKGLLGHSDADVLVHA
                     VMDAVLGALALGDIGQHFPDTDLKFHGADSLKLLSHVQGLAEEKGYVCTNLDSIIMAE
                     KPKMAPYLMEMKTNLASALKIKTEQVSIKATTTERLGLIGREEGIAAQAIVLLNSIT"
     gene            2803..3282
                     /locus_tag="EYO68_03875"
     CDS             2803..3282
                     /locus_tag="EYO68_03875"
                     /inference="COORDINATES: protein motif:HMM:PF00430.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06975.1"
                     /translation="MDFKQAIIENAWGGISVDYGMLHLQLATPAAVLIIIFIMIFTLN
                     KLLFQPIIRTLDNRKEVIVKSQKRVVQVEGEVERLKQDFQQKLNEVRSEVLHLRNVGH
                     EKGLASRESMILEERKTLQEEFENNFDELSKEIKATKTSFAKLNKELSAAISKQLLS"
     gene            3407..3976
                     /locus_tag="EYO68_03880"
     CDS             3407..3976
                     /locus_tag="EYO68_03880"
                     /inference="COORDINATES: protein motif:HMM:PF00430.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06976.1"
                     /translation="MMRLFIFVLFASFWSTALYAAGGSDGGSPLMDFVWKTLNVAVLV
                     AIIYKFARKPVASALNSTAQSAKQVVDDARSAEKKITSELSGMRIQISVLEKDAVEMV
                     ASAKKDAENEKARIIEEGRQELVRMKEQARFTLEQERRKAEDVLRNWIAEESVRLAEG
                     KLKKEMNQKHQAKLVKNYMNQLKQSEGAA"
     gene            3973..4509
                     /gene="atpH"
                     /locus_tag="EYO68_03885"
     CDS             3973..4509
                     /gene="atpH"
                     /locus_tag="EYO68_03885"
                     /inference="COORDINATES: protein motif:HMM:PF00213.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIC06977.1"
                     /translation="MSQGVIARRYAKALINLAEKDLENTGKSLTALADVFSNSAELSE
                     VLSDTKVSSQIKQNVLKKILKKIKVSKLVDTFIRYLLAKRRIVLLPNIERAFNLFLQE
                     KLGRIEAGITVAQEISEVTVGKLEKAISRYSGKEVTVNITIDPAIIGGIVTRIGSVVI
                     DGSIHTQLNQIRQSIIRG"
     gene            4516..6024
                     /locus_tag="EYO68_03890"
     CDS             4516..6024
                     /locus_tag="EYO68_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIC06978.1"
                     /translation="MKIRAEEISKIIQQQVENFDKKATKSEVGTVLEVGDGIARVDGL
                     DNVQAGELVEFPGEITGMALNLQEDNVGVVIFGSDRTIKEGDEVKRTKRIAEIPVGEG
                     LLGRVVDPLGKPIDGKGPIATKETRLIETIAPGIVDRKSVHEPMQTGLKAIDSMVPIG
                     RGQRELIIGDRQSGKTAIAIDTIINQKGGDVICIYVGIGQKRSTMAQIVQRLENEGAM
                     EHTIVVSSTASEPAPLQFLAPYAGVSIGEYFRDKGQHVLCVYDDLSKQAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERSCKLSDERGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLSTDLFNSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLRLDLAQYREK
                     EAFAQFGSDLDLATQRQLARGQRLVEILKQPQYKPMPWVDQVLSIFSATNGFLDDQPL
                     SALSKFESEFLTFIKTNKADLRKSIEKASKIDDSAEAELKSALDEFTQAFSA"
     gene            6046..6912
                     /gene="atpG"
                     /locus_tag="EYO68_03895"
     CDS             6046..6912
                     /gene="atpG"
                     /locus_tag="EYO68_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIC06979.1"
                     /translation="MASLKDIRKRIDSVKRTQKTTSAMKMVSAAKLRRSENAIREATP
                     YANKLKSVVSSLSTRFDGKDQDTGFGLLFRDTGGKRTGVILITSDRGLCGGFNSNLSK
                     ALARQLADEEVECAEFVILGRKGNEFFKRSAHNILANHTGTSPGEQLVLVRDIVSKFT
                     SRFEAGELDQVILAYNHYVSVIAQVPVIEQLLPITPPETEIADERDIIFEPSPQDILE
                     TLLKKYVQNQVYVSWLDTIAGEHAARMISMDAATKNAGEMIEKLQLHYNRSRQAAITR
                     ELIEIISGAESL"
     gene            6956..8431
                     /gene="atpD"
                     /locus_tag="EYO68_03900"
     CDS             6956..8431
                     /gene="atpD"
                     /locus_tag="EYO68_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIC06980.1"
                     /translation="MNTGKIVQVMGPVVDVAFESGDLPEIFTALEIQPKEGERIICEV
                     QQHLGESTVRTVSMGSTDGLSRGMDVKDLGETITTPVGKAVLGRIINVTGDPVDEAGP
                     IKTDVRWSIHRDAPAFDEQETEAQMLVTGVKVMDLLCPFLKGGKIGLFGGAGVGKTVV
                     IMELIRNIAAEHGGFSVFAGVGERTREGNDLWNEMKDSGVLDKTSLVYGQMNEPPGAR
                     ARVGLTGLTVAEYFRDEEAVDVLMFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLST
                     EMGNLQERITSTSKGSITSVQAVYVPADDLTDPAPATTFSHLDATVVLSRAITELGIY
                     PAVDPLDSTSRILAADIVGEEHYKVAHEVQILLQRYKDLQDIIAILGMDELSEEDKDV
                     VSRARKVQRFLSQPFFVAETFTGTAGEYVDIKDTIRGFKEILEGKHDELPEQAFYMVG
                     GIEQVQEKAKKVTDGIKTVIHQQKKLMSSEAMTTDGQSTQS"
     gene            8409..>8749
                     /locus_tag="EYO68_03905"
     CDS             8409..>8749
                     /locus_tag="EYO68_03905"
                     /inference="COORDINATES: protein motif:HMM:PRK13446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIC06981.1"
                     /translation="MANQLSLEVVTPHRTVLVEDVDSVTLPGIEGELCILPEHIPLLT
                     TLDTGIMSYKSSSGKTLAIAVHWGYAQVEGNIVRVLAELAETADEIDIERAQDAEKKA
                     KDILLTGAPTTS"
BASE COUNT         2559 a         1866 c         2029 g         2295 t
ORIGIN      
        1 cagttgctga tcatcggtgc gggacacatc acaattccgc tggtacgttt tgcaatggat
       61 ttggatttcc tgactgtcgt gattgatcca cgtactgttt ttgccaatcc ggagcgcttt
      121 cctgttcctc cggatcagct catttcaaag tggccggatg atgttttgcc ggactttcca
      181 ctcaacgagg acacatatgc cattttgctc acccacgatc cgaaaattga cgaccccgcc
      241 ttacacattt tactcaaaca gaaccttccc tacatcggtg ctctgggcag tagccggact
      301 catgccaagc gctgcgcgcg cctcaaagag gctggatttg acgctgcttc aatagcacaa
      361 ataaaaggcc cggccggagc ggacatcggt gcccagactg cggccgaaat tgcactgagc
      421 atgattacag aagtcgtggc cgcaaaacac gggaaactgt agctgttatg tttttctatg
      481 ataaacctta gtgtccctag aaacaaaacg attacggaat aatccagcct ttggaacgtt
      541 caacagcacg gttccagttt ttcatgtgct ctacagcttc atttttggaa agctgcggcg
      601 taaatgtttt ttcttcctgc cagaggctcc ggatttgtgc ctgatctttc cagacaccga
      661 ctgccaaacc ggccagaaat gcagcaccca ttgcagtagt ttcaagaacc tgtggtcgtg
      721 taactttaag tcctaataaa tctgcctgga actgacaaag aaaattattg gccgcggcgc
      781 ctccgtcaat ccgaagttct ttgacttctg ttccggtatc tttggcaatg ctgctgagaa
      841 cttctgcaga ctggtaagca attgcttcca gtgaggctcg gacaatatga ttacggtttg
      901 tatcacgggt caaacctaca atggttcccc gcgcataagg atcccagtgc ggggccccaa
      961 gaccgacaaa ggcaggaact acaaaaacac ccccggaatc tgaaactgat tcggccatgg
     1021 cctgagtttc agcagcatcc tcaaaaagct gcaatccgtc ccggagccac tgtatcaaag
     1081 caccggcgat aaaaacagaa ccttcaagtg cataagtcac ttcgttgccg atttgccagg
     1141 cgatggtggt cagcagtccg tgtttggaaa ataccagttc tgaaccggta ttggagatca
     1201 tgaagcagcc cgttccataa gtattttttg ccattcccgg ttcaaaacag gcttgaccaa
     1261 aaagggcagc ctgctggtct ccagccactc ctgcaatggg cgcttctccg ccaaacaacc
     1321 cggaattgac ctgagcaaaa acaccacttg agggtttgac ctccggaaga gtttcacggg
     1381 gtattccaaa gcgagcaaga atttcatcat cccatgaaca ggcgtcgata ttgaaaacca
     1441 gcgtcctgga agcattgctg atttcagttg catgaacctg tccgccggtc aatttccaga
     1501 tcagccagga atcaactgtg ccaaaacaaa gatttccctg gtctgcaagt ttccgagcct
     1561 gcggaacatt ttccagaatc cagcgcatct tggttccgga aaaatacgga tctgtgacca
     1621 gaccggttct gtttttcaaa atacggtcaa aaccttctgc ttttaattcc tcacaaaaat
     1681 cagcggtacg cctgcactgc cagttgatag ccggaccaat tgcatctccg gtttttctgt
     1741 tccaggcaat cgtactttct cgctgattgg taattccaac tgcagaaatg tctttcatcg
     1801 acagtttggc ctgcgctaag gctgcctcta tagtttgtcg ctggctgctc cagatttctt
     1861 ccggaacctg ctcgacccag ccggattgcg gataatgaca taccaatgga gcatttgctt
     1921 cggcaacaat ttttccgttt tcatcaaaca gaatggtacg ggaactggtg gtgccctgat
     1981 ccagagccat gataatttgt gacatatttc ccctctatta agttatggaa ttaaggagga
     2041 caattgcctg agctgcgatg ccctcttcgc ggccgatgag acccagtcgt tctgttgttg
     2101 ttgccttgat agaaacttgt tcagtcttaa tcttgagggc agaagcaagg tttgttttca
     2161 tttccataag ataaggggcc atttttggtt tttcagccat gatgatgctg tcaagatttg
     2221 tacagacata gcccttttct tcagctaaac cctgaacatg actgagaagt ttcaggctgt
     2281 ctgctccgtg aaattttaag tctgtgtccg gaaagtgctg tccgatgtct cccagggcaa
     2341 gtgcacctaa gacggcatcc ataacagcat gcacaaggac atctgcgtcc gagtgtccca
     2401 gaagaccttt tggatgagga atttgtacgc ctcctataat cagcagccta tcctcagcaa
     2461 gtgtgtgtac atcaaaacca attcctgttc tatacattct tacaccagat tatacctgtt
     2521 atgcattttt aactcgtgag tcatgagttc tcgtaagata ctaatattat tgactacaac
     2581 ttgtcatctc gacaaaagag cagggtgctg atatcgtgca agatcccttg cattcgttcg
     2641 gggtgacaca acaaaactaa tgactgtcga gatattttta aaagaaaaat gattatacca
     2701 tcctaaaaat taaatagaaa tgtgcttgac attgtttata attatatgct aaaaaatatg
     2761 gtttagctta attaattttc ttgcaaaaaa gggtagcctc agatggattt caaacaagca
     2821 attattgaaa acgcatgggg tggtatcagc gtggattatg ggatgctgca cctgcagctt
     2881 gctacgcctg ccgcggtact gatcataatt tttattatga tttttacact taataagctt
     2941 ctttttcaac caattatacg aaccctggat aatcgtaaag aagttattgt caaaagtcaa
     3001 aaacgtgttg ttcaggtcga aggtgaagtt gaacgcctca agcaggattt tcaacaaaaa
     3061 ctgaatgagg tgagatctga agttctccat ctgcgaaatg ttggacatga aaaagggctg
     3121 gcctcaagag agtccatgat ccttgaggaa agaaagactc tacaggaaga gtttgaaaat
     3181 aattttgatg aactttctaa agaaataaaa gctaccaaaa ccagttttgc aaaactgaat
     3241 aaagagttat ctgctgcgat cagcaagcag ctgttaagct gaaaaaagta ctctgtcaat
     3301 gcataacaaa ataagcaatt caggcaatca catttgaacc accggaattg tacgggtgtt
     3361 cctctcagcc agtatctctt gttgaaaaaa tgatacagtg aaaaaaatga tgcgactttt
     3421 tatatttgta ctgtttgcct ctttctggag cactgctctc tacgcagctg gaggaagcga
     3481 tggcggttcg cctttaatgg actttgtctg gaaaacactc aatgttgctg tacttgttgc
     3541 catcatttac aaatttgcca ggaaacctgt agcatcagca ctcaacagta ctgctcagtc
     3601 tgcaaagcaa gtggttgacg atgccagaag tgctgaaaaa aaaatcactt ccgaattaag
     3661 tggaatgcgt attcaaatat ctgtccttga aaaagatgct gttgaaatgg tggcatctgc
     3721 aaaaaaggat gctgaaaatg aaaaagctcg aatcattgaa gaaggcagac aagagctagt
     3781 ccgcatgaag gagcaggccc gatttaccct ggaacaagaa cgcagaaaag cagaagatgt
     3841 tttgcgtaac tggattgccg aagaaagtgt caggttggct gaaggaaagt taaaaaagga
     3901 gatgaatcaa aagcatcagg caaaactggt aaaaaattat atgaatcaac tcaaacagtc
     3961 tgaaggagcc gcttgagcca gggagtcatt gcacggcgct atgccaaggc cttaattaat
     4021 ttggcagaaa aagaccttga aaatacaggt aaaagtttga ctgcattagc cgatgttttt
     4081 tcaaactccg cagagttaag tgaagtcctt tccgacacta aagtttcctc acagatcaag
     4141 caaaatgttc tgaaaaaaat cctaaaaaaa ataaaagttt ctaaacttgt tgacacattc
     4201 atccgctacc ttttggcaaa gcggaggata gtattgctcc caaatattga gcgggctttt
     4261 aatttgtttt tacaagagaa actaggccgt attgaggccg gaataactgt ggcacaagaa
     4321 atatccgaag taactgttgg caaattggaa aaagctattt ccaggtattc cggaaaagaa
     4381 gtcacagtta acattaccat cgaccctgcc atcattggcg gaatcgtgac gcgtatcggt
     4441 tccgtggtaa ttgatgggag tattcacacg cagttaaatc agattcgaca atcaattatc
     4501 agaggctgag tggctatgaa aatccgagca gaagaaatta gtaaaattat tcaacagcaa
     4561 gttgaaaact ttgacaaaaa agctaccaaa tcagaggtcg gaactgtgtt ggaagttgga
     4621 gatggaattg cccgtgttga tggcttggac aatgtccagg ccggggaact cgtcgagttt
     4681 ccaggggaaa ttacaggcat ggctctaaac cttcaagaag ataacgtggg agttgtaata
     4741 tttggaagcg accgcaccat caaagagggt gacgaagtta agcgaacaaa gcgtattgca
     4801 gaaattcctg tgggcgaagg cctgctggga agagttgtcg atcctcttgg aaagcctatt
     4861 gacggcaaag gtccaattgc tactaaagaa acacgtttga ttgaaaccat tgctccaggc
     4921 attgttgaca gaaaatctgt gcatgaacca atgcaaacag gactgaaagc gattgactca
     4981 atggttccaa tcggtcgtgg tcagcgggaa ttaattattg gcgacagaca gtctggaaaa
     5041 acagcaattg ccattgacac catcattaat caaaaaggcg gtgatgtaat ctgcatctat
     5101 gtaggaatcg gtcagaaacg atctacaatg gctcagattg ttcagagact ggaaaacgaa
     5161 ggtgcaatgg aacacaccat tgttgtttca tctactgctt ccgaacctgc tccattacag
     5221 tttttagcac catacgcagg tgtgagcatc ggcgaatatt tccgtgataa gggacaacac
     5281 gtgctctgtg tttatgatga tctctccaag caggcagttg cctaccgtca gctttctttg
     5341 ctgctgagac gaccgcctgg acgtgaggct tatccgggtg acgtttttta tcttcacagc
     5401 cgtttacttg aacgatcctg caaattgagt gatgaacgtg gcgcaggctc tttaactgcc
     5461 ctgccaatta ttgagactca ggccggagac gtttctgcat atattccaac taacgtaatt
     5521 tcaattacag atggacaaat ttttctgagc accgatcttt tcaactcagg aatacgtcct
     5581 gcaattaacg taggtctgtc ggtttcccgt gttggtggtg cagcccaggt aaaggcaatg
     5641 aaacaggttg ccggaacttt acgtctggac ctggcccagt acagagaaaa agaagccttt
     5701 gcccagtttg gaagtgactt agatctggct acccaacgac agttggcacg agggcagcgt
     5761 ttggtggaaa tcctcaagca accacaatac aaaccaatgc cctgggtgga ccaagttctt
     5821 tcaatttttt ctgcaaccaa tggattttta gatgatcagc cgttgagtgc cctgagtaaa
     5881 tttgagtctg agttcctgac ttttatcaaa acaaataaag ctgatttacg taaatcaatt
     5941 gagaaggcaa gcaaaattga tgactctgct gaagctgagc tgaagtctgc tttggatgaa
     6001 tttactcagg ctttttcggc ctaacacacc gtgtattaat ttagaatggc cagcctaaag
     6061 gatatacgta aacgcattga cagcgtaaaa agaactcaga aaactaccag cgccatgaaa
     6121 atggtgtctg ctgcaaaact gagacgctca gaaaatgcca ttcgggaagc aacaccttat
     6181 gcaaataagc tgaagagtgt tgtttcttcg ctcagcacac gtttcgacgg taaggatcag
     6241 gacacaggct tcggcctcct tttccgggat acgggcggta aacgtacagg tgtaatttta
     6301 attacaagtg accgcggtct atgtggaggt ttcaactcaa atctctctaa agctttagct
     6361 cggcagctag cagacgagga agttgagtgt gctgagtttg tgatccttgg tcgcaaagga
     6421 aacgaatttt tcaaacggtc agctcacaac attctggcta atcatactgg gacttctccg
     6481 ggggaacagc tggttcttgt ccgtgatatt gtgagtaaat ttaccagtcg ttttgaagcc
     6541 ggcgagttag atcaggtcat tcttgcatac aatcattatg tgagtgtgat tgcccaggtt
     6601 ccagttattg aacaactctt gccaataaca ccgcctgaaa cagaaatagc agatgagcgg
     6661 gatataatat ttgaaccttc tccgcaagat attttagaaa cgctgctaaa aaaatatgtt
     6721 cagaatcagg tatatgtttc ctggcttgat acaatcgccg gagaacatgc tgcgcgtatg
     6781 atatcaatgg atgcagcaac caagaatgcc ggagaaatga ttgaaaaatt acaattacat
     6841 tataatcgaa gcagacaggc ggcgatcact agagagttga tcgaaattat aagcggtgca
     6901 gaaagtttat aatacagaaa ataattatcc accaaactaa aacaaagagg gaacaatgaa
     6961 cacaggtaag atcgtccagg tgatgggacc cgtcgttgac gtggcatttg aatccggaga
     7021 cctccctgaa attttcacag cacttgaaat tcagccaaaa gagggcgaac gaattatctg
     7081 cgaggtgcag cagcatcttg gagaaagtac tgtacgtact gtttcaatgg gatcaaccga
     7141 cggtctttcc cgtggaatgg acgtgaagga tttgggtgaa acaattacta cacctgttgg
     7201 gaaagcagtg ctgggacgga tcattaatgt aacaggcgat ccagtagacg aagccggtcc
     7261 gattaagaca gatgtacgct ggtcaattca ccgcgatgct cctgcatttg atgaacagga
     7321 aactgaagca caaatgctcg taactggagt caaagtgatg gaccttctct gtccctttct
     7381 caaaggcggt aaaatcggtt tattcggtgg tgcaggagtt ggtaaaaccg tggtgatcat
     7441 ggaactgatc cgcaacattg ccgcagagca tggtggattt tctgtttttg ccggagttgg
     7501 tgaacgtaca cgagagggga atgatctctg gaacgaaatg aaagattccg gtgtgctgga
     7561 caaaaccagc cttgtttacg gacaaatgaa tgaacctccc ggggcacgtg cacgtgtagg
     7621 attgacaggt ttgactgttg cagaatattt ccgtgacgaa gaagctgtag atgtgttaat
     7681 gttcatcgac aatattttcc gctttacaca ggcaggttca gaagtttccg cattgcttgg
     7741 tcgtattcct tctgcagtgg gttaccagcc tactctttcc acagaaatgg ggaatttgca
     7801 ggagcggatt acgtcaacca gtaaaggttc tattacttct gtacaggcag tttatgttcc
     7861 tgcagatgac ttgactgacc ctgcacctgc aacgacattt tcgcatctgg acgcaaccgt
     7921 ggttttaagc cgtgcgatta ctgaacttgg aatttaccct gcagttgatc ctcttgattc
     7981 tacttcacgg attcttgcag cggatattgt tggcgaagag cactataaag ttgcacacga
     8041 agtgcaaata ctgcttcagc gctacaaaga tttgcaggac attattgcaa ttctcggcat
     8101 ggatgaatta tctgaagaag acaaggacgt tgtgagccga gccagaaaag tacaaagatt
     8161 tttatcgcag cctttctttg ttgcagaaac atttaccgga accgctggag agtatgtgga
     8221 cataaaagac acaatccggg gattcaagga aattcttgaa ggaaaacatg atgaacttcc
     8281 agaacaggct ttctacatgg ttggtggaat tgagcaagtg caggaaaaag ctaaaaaggt
     8341 gacagatggt ataaagacgg ttattcatca acagaaaaaa ttaatgagct cagaagcaat
     8401 gacaactgat ggccaatcaa ctcagtcttg aagtagtaac acctcatcgt acagttctgg
     8461 ttgaggatgt agattcagtg actttacctg gtatcgaagg tgaattgtgt atcctgcctg
     8521 aacatatccc tctactgact acacttgata ctggaattat gtcttataaa agcagcagtg
     8581 gaaagacact ggccattgct gttcactggg gctatgccca agttgaaggg aacattgtta
     8641 gggtgctggc agaactggcc gaaaccgctg atgaaattga catagagcgt gcacaagatg
     8701 cagaaaaaaa agcaaaggac attctcctca ccggtgctcc tacaacttc
//
LOCUS       DTVD01000109            2195 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_21677_length_2195_cov_591.496262, whole genome
            shotgun sequence.
ACCESSION   DTVD01000109 DTVD01000000
VERSION     DTVD01000109.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21677_length_2195_cov_591.496262"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(168..725)
                     /locus_tag="EYO68_03910"
     CDS             complement(168..725)
                     /locus_tag="EYO68_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIC06982.1"
                     /translation="METVSFTKMEDGTKEEYAFLEPLYIQCREGIPEMLLGLLKRMQG
                     DRLGYQIDRYQHSLQTATRAERDGSDEETIVCALLHDIGDVLAPDNHSQVAAAILQPY
                     ISERNYWVLKHHGLFQGYYYFHHINKDRNIRDNFKDHQFYQACVDFCSQWDQCSFDPD
                     YDTLPLEHFEPMVGRVISEPWNHFD"
     gene            complement(743..1840)
                     /locus_tag="EYO68_03915"
     CDS             complement(743..1840)
                     /locus_tag="EYO68_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HIC06983.1"
                     /translation="MIKKDESVEITWKNGETTLLPNLWLRDNCACDVCRNTQTTEKLF
                     QLAQVDVNLMPETVDLIEDELHLLWPDGHRSVYEGSAIRALTIPQTTHLRLWSSEFRP
                     QQFDYGKFTHNDSIAVDAIENFLSTGTILLTNAPTTESLEYLTPRLGPLRETVFGRIH
                     NVVVDSTGYNVASTNLELPPHTDLSSYSWPPSAQALHMLVNETEHGESIIVDGFAVAT
                     QFRNDHPNWFDRLCCTPVPFRIFDDNNETFATQPIIQLDTGGQIAGVRYSNQTMQPMN
                     PVRTGLNEFYSAYHEFSTRITSDKAKAIFRLEGGQILLVAGHRVLHARKEFVANAKRH
                     LQDAYFEHDNIRNHLTVIQRRLRKIGNTSLQ"
     gene            complement(1893..>2195)
                     /locus_tag="EYO68_03920"
     CDS             complement(1893..>2195)
                     /locus_tag="EYO68_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06984.1"
                     /translation="EGERCAEMLNDPKIKVMIMGNHGVLVIGSNVADTFNRLYYFERA
                     AETYIKALWTGQPLRLLSDEIAEKTAGQLENYPDFSQIYFEELKAILDEKEPTYRL"
BASE COUNT          595 a          489 c          468 g          643 t
ORIGIN      
        1 ccgtgtttcg cctccagatt ttctaattgc tctctgtggt cggcggcttc ttctgctgaa
       61 aaaattctca gaggaaagaa aaaaccgtcc cgtcgatatt gttcttgatc tgcttggctg
      121 agttgatggc tcatatcgtt tttttcggct gagtcagttt ttcttcatca atcgaagtgg
      181 ttccacggct cgctgataac acgcccaacc attggttcaa aatgctctaa aggcaaggtg
      241 tcgtaatccg gatcaaatga acattgatcc cattgggaac agaaatcaac acacgcttga
      301 taaaactggt gatccttgaa attatcccgg atgttgcggt ctttattgat gtgatgaaaa
      361 tagtagtaac cttgaaacag gccgtgatgt ttgagtaccc aataattgcg ttcgctgatg
      421 taaggttgaa gaatggcggc ggcgacttga ctgtgattgt ccggagctaa aacatcaccg
      481 atatcatgca gcaaagcaca gacaatggtt tcttcatccg aaccatcccg ttcagctcga
      541 gtggccgttt gtagagaatg ctgatagcgg tcgatttgat agcccaaacg atctccctgc
      601 attcgcttga gcaatcccaa gagcatctcc ggaattcctt cgcggcattg aatataaagc
      661 ggctctagga aagcatattc ctcctttgtg ccgtcttcca tttttgtgaa gctgactgtt
      721 tccataaatt cctccggaac tattattgca gggatgtatt accgattttt ctcaatctgc
      781 gttgaattac agttaagtgg ttgcgaatgt tatcgtgttc aaagtaagca tcctgcaaat
      841 gtcgtttggc atttgctaca aattctttgc gagcatgtaa cactcgatgc cctgcaacga
      901 gtaaaatttg ccctccttcc aaacgaaaaa ttgcttttgc cttatcactg gtaatacggg
      961 tagaaaactc atggtaagcg ctataaaact cattgagccc ggttcgtacc ggattcattg
     1021 gttgcatagt ttgattggaa taacgcactc cggcaatctg tccgcctgta tccaattgaa
     1081 tgataggttg agtggcaaaa gtttcgttgt tgtcatcaaa aatcctgaat ggaaccggag
     1141 tacagcataa acgatcaaac cagtttggat gatcgttacg gaattgggtt gcgacagcaa
     1201 atccatccac aataatcgac tctccatgtt ccgtttcatt gacaagcatg tgcagagctt
     1261 gagcactggg aggccaggaa taactagaca aatcggtatg cgggggcagt tctaagttcg
     1321 tactggcaac attgtaacct gtggaatcca ccacaacatt atgaatcctt ccgaaaaccg
     1381 tttcccgtaa cggccccaac cttggagtaa gatactccaa actttctgtt gttggagcat
     1441 tggtcagtaa aatagtacct gtgctcagaa aattttcaat cgcatcgaca gcaatgctat
     1501 cgttatgtgt aaactttccg tagtcaaatt gctgaggacg aaactcactg ctccaaagcc
     1561 gcagatgagt tgtttgggga atcgttaaag cgcgaatagc tgacccttca taaactgacc
     1621 tgtgtccgtc aggccaaaga agatgcagtt catcttcaat cagatcgaca gtttctggca
     1681 tcaagttaac atcaacttgg gccaattgaa acaacttctc tgttgtttga gtgtttcgac
     1741 acacatcgca agcacaatta tctcgcaacc acaaattcgg aagcagggta gtctctccgt
     1801 ttttccaagt gatttcaaca gattcatctt ttttgatcat gattcttagt ccgaaatatc
     1861 attttgaagt gattcgatcc atgttaaaaa gtttacaagc gataagtcgg ttctttttcg
     1921 tcaagaatag cctttagttc ttcaaaatat atttgactga aatccggata attctccaat
     1981 tgtccagcgg ttttttctgc aatctcatca gaaagcaagc gtagcggctg tcctgtccag
     2041 agtgctttga tgtaagtttc cgcggcccgt tcaaaataat aaagccgatt gaaagtgtcg
     2101 gccacatttg agccgataac aagtacaccg tgattgccca taatcatgac tttgatctta
     2161 ggatcgttga gcatttcagc acaccgctct ccctc
//
LOCUS       DTVD01000110            8734 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2168_length_8734_cov_352.478050, whole genome
            shotgun sequence.
ACCESSION   DTVD01000110 DTVD01000000
VERSION     DTVD01000110.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_8734_cov_352.478050"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(75..1298)
                     /locus_tag="EYO68_03925"
     CDS             complement(75..1298)
                     /locus_tag="EYO68_03925"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC06985.1"
                     /translation="MLTLRLAFRNLLRHKSRSLLCALSMMGAYVLFSISLGFSDGSYG
                     SLIRMLTDTHSGHVQIHSQGYLERPSLFRNFILYPALKNQLTADPEIKAWTARVFSAA
                     LIFRENKSTIARLMAIDPEQEAQVTTMREKVQDGEYLSEAADVTNPILLGRHLQKLLQ
                     AKLGETLIFIGQGADGSIANDLFTVVGIVGKSSADAESRMIYMTLESAQEFLSLGERI
                     HEIALRLENYSEARRQAAYLQNYLKAKELDVQPWQVVEEQFYKAMLADQQGNWITQAV
                     IMLIVGLVVLITVLMNTFERRHEYGLLLALGTPPGFIFRSIIVEMTMLSGLSVIVGLF
                     FSSGLNWYFSINGIAIEPPMEYGGVIFSAISSEVSLFVIFVPAVFTIGVAFLVAFFPA
                     IKSARAVPIEAMREN"
     gene            1336..1377
                     /locus_tag="EYO68_03930"
     CDS             1336..1377
                     /locus_tag="EYO68_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06986.1"
                     /translation="MFFENTGLKSIDL"
     gene            complement(2160..3287)
                     /gene="queG"
                     /locus_tag="EYO68_03935"
     CDS             complement(2160..3287)
                     /gene="queG"
                     /locus_tag="EYO68_03935"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIC06987.1"
                     /translation="MRTPTLTSLIRKKASELGFDLIGAIPVSRSKTIDIYNAWLKKGY
                     AGSMDYLERHSVLKEDPRQLLPETISLLALGFNYCTVDPPALVHNPEIGCISRYAWGD
                     DYHELIRTKLRVLENYICQELNAGNLSRSFVDSGPVLEREIAQRAGLGWIGKHSNLIN
                     MEKGSWFFLAELLVDVPLETNLPFTRVDCGTCTICIEACPTEAIIADRTVDARLCISY
                     LTIELKGAIPVELRPKMSNMIFGCDICQEVCPWNQNASKSPESGLRPRPENVAPELTE
                     LMKLDQSAFKKRFRKSPIKRTKRRGLLRNVAVALGNWAHESAIPALSLGLQDSEPLVR
                     SHAAWALGRISASQAKINLKAAKITEKDPAVLEEIEAALGA"
     gene            complement(3295..4503)
                     /locus_tag="EYO68_03940"
     CDS             complement(3295..4503)
                     /locus_tag="EYO68_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HIC06988.1"
                     /translation="MPLSPPQTQHLDSILPAEPLLLMGAGPVPISHAVSRANGVVINH
                     LGETMDAVVSNVKMMGRYAFQTVSDKIIGVSGPASAAMEMAITNILWPGRSVLALSMG
                     TFSGRMAEMAEGVGAEVTVLKSTGIEPVRLEQIREALSKKHYDVITMAQGETSCGVEM
                     KCIPEVAQMAKELGTLVVVDAVCTLTTMPLQMDEWGIDIAIAGGQKGLSSIPGVSLIV
                     FSEAAWKTIESRNVRQPHWCLDALKAQTFWGSQQYHYTAPVPGILALHEALRQICEET
                     LPSRFERHRTSSIALQSGIETMGLKLFVPIKDRLNSVVAIQTPEDTDSAQIRKIMSKR
                     FNVEISGAFGMDIMRIGQMGEQCRSHNLFKVLYALGMSCRQTGVDLDVSLGMAELELN
                     LVIDPENLVD"
     gene            complement(4619..8269)
                     /locus_tag="EYO68_03945"
     CDS             complement(4619..8269)
                     /locus_tag="EYO68_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC06989.1"
                     /translation="MNSPSQEKTDLPEILRKTIALAEKWQNRANTLRTKDELKQQQMM
                     RRLLNHPSDKTVVMHLVDQSFRSGNPRRAVDQFRYLLEHHGIPRFFPQVEQWLLKIFL
                     RFGNILPQISHSQILRKIRADTRRTILPEEPEILKAHLNKRQVGGVQVNLNHLGEAVL
                     GDGEALNRLKHYGESLRNPDIHTISIKISNIVAQLHPLGFENELDLICERLSELYRNA
                     SENPVLHSDGTRRAKFVNLDMESYHDLDLTMAAFMRTLDQAEFKNLQAGIVLQAYLPD
                     SFLRQQELVKWAKKRVKNAGSPVKMRLVKGANLVMEAVEAEIFGWPLTVFESKIETDA
                     NFKRMLEFGLQPENIAAVHFGLASHNLFDLAYTHVLAEERGITEGLVFEMLEGMADHV
                     RRTLQEEKRKAKTKETNLLLYTPVTSQDQFISAIAYLVRRLDENTSPDNFLRHSFDIR
                     VGSSEWKFLENQFLEAWQLKDKISSEPRRQQNRDKEVFPENLGTFADSIFRNEADTDW
                     VLPENRKWADKIRKKWQPTANTKVKKIPLYIGNSEVKSKRKQQLFFDPSQPAADVPAV
                     YSVALATKTDVKKAVKIAKDDPEQWRHTTLSERHELLSGAALNMRKRRGDLIGAAASV
                     CGKTFYESDPEISEAIDFIEYYPHSLRLIEQHTSLKLSPLGVVLVIPPWNFPIAIPTG
                     GVAAALACGNTVLFKPSPLAFPLGAEIAKCFWDAGIPREALQLVYCENGEPIQTLSGH
                     PDVDAIIFTGGTQTALKLLEKRPDAELSAETGGKNATIVTAMADRDQTVEHVLHSAFS
                     HSGQKCSATSLLVLEKEVYEDETFRKQLLDAIQTLKVGSVWNFSNKMGPLIREPMADF
                     RKGLETLEDGESWALQPKCDERNLKLWHPAVKWGVKRGSFSHQTEFFGPLLSVMKAES
                     LDDAIEIVNDTNYGLTSGLESLDPREQKIWSEKIKAGNLYINRVTTGAIVLRQSFGGM
                     GLSAIGAGIKAGSPNYAVQFCRIEESKPYGDSPTQGPLREESPLLYLARNWQTQLSRN
                     EHAEIWSELHKTIQAIYSCLFQYESEFSGEQDFFRFRGQDNLFRYLPLGKVTVRLHPD
                     DELFETLIRIAAARIAKCKVEVSFPPNLENIVTEFLGTTEGKRLCDSVEIRSETDKEL
                     CGRLTEIDRIRYAHPDRVPLEIHQATAKLGKHISRHIPLAEGRIEMLRYLQEQSLSID
                     YHRYGNLGEREF"
BASE COUNT         2356 a         2054 c         1850 g         2474 t
ORIGIN      
        1 gttttcggta attcttagtg ccttagtgcc ttagtgcctt agtgccttag tgcctcagtg
       61 cctcagtgcc ttttttaatt ctccctcatt gcttcaattg gaacagccct ggcagacttt
      121 attgcaggaa aaaaggctac caaaaaggct actccaattg tgaaaaccgc cggaacaaaa
      181 atgacaaata acgagacttc actactaatt gcactaaaaa taacacctcc atattccatc
      241 ggtggttcaa ttgcaattcc attgattgaa aaataccagt tcaatccact ggaaaaaaac
      301 agaccgacaa taacactgag accacttaac attgtcattt caacaatgat tgaacgaaaa
      361 ataaatcccg gtggagttcc caatgccagc agcagcccat attcatgacg acgttcaaag
      421 gtattcatta aaactgtgat caaaactacc aagccaacta tcagcatgat aacggcttgt
      481 gttatccagt ttccctgttg atcggcaagc atcgctttgt aaaattgttc ctcaacaacc
      541 tgccagggtt gcacatcaag ttctttagct ttaagatagt tttgcagata tgcagcctgt
      601 ctgcgggctt cggaataatt ttcaagacgc agtgctattt catgaatcct ttctcccagt
      661 gacaaaaact cctgtgcaga ctcaagagtc atgtagatca tacgcgactc agcatctgcc
      721 gaactttttc cgactattcc aactactgta aacaaatcat tggcaattga accgtctgca
      781 ccctgaccga tgaaaatcag tgtctcacca agtttggcct gaagcaattt ttgtaaatgt
      841 cttccgagca ggattggatt tgtcacatca gcggcctcac tcaaatattc tccatcctgg
      901 actttttcac gcatcgtcgt cacttgcgct tcctgctcag gatcaatggc catcagtctg
      961 gcaatagttg atttgttttc cctgaagatc agcgcggcag aaaaaactcg tgcggtccag
     1021 gcttttattt caggatctgc tgttagctga ttttttaagg caggataaag tataaaattg
     1081 cgaaaaagtg aggggcgttc cagataaccc tgactatgga tttgcacatg tccagaatga
     1141 gtatcagtca gcatacggat taaactgccg tagctgccgt cactgaaacc tagtgaaata
     1201 gaaaaaagca cgtacgctcc catcatgctc aacgcacaga gcaatgaccg acttttatgc
     1261 cgcagcagat tacggaaagc aagtctcaat gtcaacatta gtagctattt tcgtcttaat
     1321 tgactgaaag aaaatatgtt ttttgaaaat accggcttaa aatcaattga tttataacga
     1381 attaaagtcc tgctgttttt gttcagtgta cgcatttcca tcacagcagg aatcattttt
     1441 ccgcccattt gctgaattcc actaaattcc atgacccgtt tcttttcacc atcttcattg
     1501 taataaattt gctcaaccgg cataaaatct tggcggcgaa tactgatcga tattttaccc
     1561 cagactgtaa ccgtttcatt ttttggtctg agtacaataa agtagaattg attaggatct
     1621 gaaggatgtt cttccagttt tgcgtgataa tcttctgcaa gcgtgttttc tttgaccaaa
     1681 tcatcgttgg taaagtcaga acccatccac gaattcatca tcattgaagg cggaaccttg
     1741 aggaccttat ttattttagg aaaataattc cacatctgat taccgagctt cagtgttttt
     1801 atacctttat ctttgcgtgg tgagagaatt ttaatcagcg tgtcattcat tcctctcgac
     1861 cagacttcca taacagtcgt acgttcccag ttttcagtca caatcttcat ttccatggtg
     1921 gcatgacttg aatcacttcg gtaaagccgg tccaccttat ccagcagacc ttctacagaa
     1981 gtttcagcaa gcacggaaaa agggaataca aaaacaaaaa ttgcaatgcc aaagatttgt
     2041 ttcatgatta gtgtactgta cagttttgtt tgttgaacaa ataaacaacc attgttttcc
     2101 cacaagaacc aaattaatac aattagtgaa tatacccggt cctgctaatt ttcatagaat
     2161 caagcaccaa gggcagcttc aatttcctct aaaactgcag gatctttttc ggttattttt
     2221 gcagctttca aattaatttt agcctgtgac gcagaaatac gacccaaagc ccacgcagcg
     2281 tgactgcgta ccagaggttc ggaatcctga agccccagac tcagggcagg gattgcagac
     2341 tcatgagccc agtttcccag tgctactgca acgttacgta gtagtccacg acgttttgta
     2401 cgcttgattg gacttttacg gaaacgtttt ttaaaagcag actgatccag cttcatcaat
     2461 tcagtcagtt cgggagcaac attttcagga cggggccgaa gtccagattc tggtgatttt
     2521 gaagcattct ggttccaagg acagacttcc tgacaaatgt cacagccaaa aatcatgtta
     2581 ctcatttttg gtctcagttc aacaggaatt gcccccttga gttctattgt caaataagaa
     2641 atacagagac gtgcatctac tgtacgatct gcaattatgg cctcagtagg acatgcctca
     2701 atgcaaattg tgcatgttcc acaatccact cgtgtaaacg gcaaattcgt ttccagcgga
     2761 acatcaacca gcaattctgc aagaaaaaac catgagccct tttccatgtt tatcaaatta
     2821 gaatgtttgc caatccagcc cagaccagca cgctgcgcaa tttcacgctc cagcacaggt
     2881 cctgaatcaa cgaaactccg cgaaagattt ccggcattta attcctggca aatatagttt
     2941 tccaaaaccc tcaactttgt acgaataagc tcgtgataat catcacccca tgcataacgg
     3001 ctgatacagc caatttcagg attgtggact agtgcgggag gatcgacggt acaatagtta
     3061 aaaccaagtg caagcagaga gattgtttcc ggaagcaatt gacgtgggtc ttcctttaaa
     3121 acggaatggc gctccagata atccattgaa ccggcataac cttttttgag ccacgcattg
     3181 taaatgtcaa tggttttgga gcgtgaaaca ggaattgcgc caattaggtc aaaaccaagt
     3241 tcgcttgctt ttttccgaat gagactggtg agagtggggg ttctcattca attttcaatc
     3301 cactaaattc tcaggatcaa tcaccaaatt gagctccagc tcagccatcc ccaggctcac
     3361 atcaagatct acgcctgttt ggcgacagct cattccgagg gcatagagta ccttgaatag
     3421 attgtgggaa cgacactgtt cgcccatctg accgatacgc attatgtcca taccaaaggc
     3481 tcctgaaatc tcaacattga aacgcttcga catgatttta cggatttggg cactgtcggt
     3541 gtcttctggt gtttggattg ccactacgga attgaggcgg tcttttatcg gcacaaagag
     3601 tttcaatccc atggtctcaa ttcctgactg caaagctata gaactggttc tgtgacgttc
     3661 aaaacgcgaa ggcagagttt cctcgcaaat ttgccgcagg gcttcatgca aagccaatat
     3721 tccaggaact ggagcagtat agtggtattg ttgtgatccc caaaatgttt gtgctttcaa
     3781 ggcatccagg caccagtggg gttggcgtac atttcgagat tcgattgtct tccatgcagc
     3841 ctcagaaaat acaatcaggg agactccggg aatcgaagag agaccttttt gacctccggc
     3901 aatagcaata tcaattcccc attcgtccat ctgcaagggc atggtggtta atgtacaaac
     3961 cgcatccaca accaccagtg taccaagctc tttagccatc tgagcaactt cagggatgca
     4021 tttcatttca acaccacagg atgtttcgcc ctgagccatt gtgataacgt catagtgttt
     4081 tttggagagt gcttcccgga tttgctccaa acgtacaggt tcaattccgg ttgatttaag
     4141 cacagtcact tctgcgccta caccttcagc catttctgcc atgcgtccac taaatgttcc
     4201 catggacaag gctaaaacac ttcgacccgg ccagagaatg tttgtgatag ccatttccat
     4261 tgccgctgaa gcaggtccgg aaactcctat gattttatct gaaacagttt ggaaagcata
     4321 acgccccatc atctttacat tgctcacaac tgcatccatg gtttcgccca gatgattgat
     4381 caccactcca tttgctcggg aaaccgcatg agaaatcggg acgggtcctg cacccatcaa
     4441 cagcaatggt tctgcaggta ggatggaatc taagtgttgt gtttgtggag gagaaagcgg
     4501 catgttgaaa agatccgtta agatggcagt tttgcctttt ttcctttgca atttctacgc
     4561 tcaaggtatg aaattgacta caatcttttg cattttcaga aaaatatcaa aggaactctc
     4621 aaaattcccg ctctcccaaa tttccgtagc ggtgataatc gatggacaaa ctttgctcct
     4681 gtaaataacg caacatttca atacgtcctt cagctagcgg aatgtgacgg gaaatatgtt
     4741 tgccaagttt tgctgtggct tggtgaattt caagtggaac tcggtcggga tgtgcgtagc
     4801 ggattcgatc aatttcggta agtcttccgc agagttcttt gtcggtttca gagcgaattt
     4861 caacagaatc acataagcgt tttccttcag tagtgcccag aaattcagtg acgatgtttt
     4921 ccaaatttgg tgggaaactg acttccactt tacattttgc aattcgtgcg gcagcaatcc
     4981 tgatcagggt ttcaaagagt tcgtcgtccg gatgcaagcg cacggtgact tttccgagtg
     5041 gtagatagcg aaacaaattg tcctgtcccc gaaaacggaa aaaatcttgc tcaccagaaa
     5101 attcgctttc gtattgaaaa agacagctgt aaatcgcctg aatagtttta tgtaattcac
     5161 tccagatttc agcatgttca tttcgcgata gctgagtctg ccagtttcga gcaagataaa
     5221 gcagaggtga ttcttcacgc aacgggcctt gagtcggcga atcgccgtaa ggcttgcttt
     5281 cctcaatccg gcaaaactgc acggcgtaat tcgggcttcc ggctttgatt ccggcaccga
     5341 tggcagaaag tcccataccg ccaaacgact gacgcaggac aatcgcaccg gtcgtcacgc
     5401 ggttgatgta gaggttaccg gctttaattt tttccgacca gattttttgc tcccgcggat
     5461 ccaaactttc gaggccggaa gtcaaaccgt aattcgtgtc attgacaatt tcaatggcat
     5521 catccaaaga ttctgctttc atcaccgaaa gcaacggtcc gaaaaattct gtttgatgtg
     5581 aaaaactgcc gcgtttcacg ccccatttta ctgcaggatg ccaaagtttt agattccttt
     5641 catcacattt tggctgcaag gcccagctct ctccgtcctc caaagtttcc aagcccttac
     5701 ggaaatcggc catcggctcc cgaataagcg gtcccatttt atttgaaaaa ttccagaccg
     5761 aacctacttt caaagtttga atagcatcca gcaattgttt gcgaaaagtc tcgtcttcat
     5821 aaacttcttt ttccaaaaca agcagcgaag tcgctgagca tttttgtcca ctatgactga
     5881 atgcggaatg caaaacgtgt tcgactgtct ggtctcgatc ggccatggca gttacgatgg
     5941 ttgcgttttt acctcctgtt tccgcggaca gttctgcatc aggacgcttc tccagcagct
     6001 tcaaagccgt ttgtgtccct ccggtaaata ttattgcgtc cacatccgga tgtcctgaaa
     6061 gcgtttgaat cggctctccg ttttcacagt ataccagctg cagggcttct cgcggaattc
     6121 cggcatccca aaaacatttt gcgatctccg ctcctaaagg aaaagccagt ggtgaaggtt
     6181 tgaacagaac ggtgtttccg caagccagcg cagcggccac tcctccggtc ggaatcgcaa
     6241 tcgggaaatt ccaaggcggt attaccagaa ccacgccaag cggtgacaat tttaacgatg
     6301 tatgctgttc tatcaatcgc agagaatgcg gataatattc gataaaatca attgcttccg
     6361 aaatttccgg atcactttca taaaaagttt ttccgcaaac ggatgcagct gcgccgatta
     6421 aatcaccccg ccttttacgc atattcagtg cagctcctga aagcaattcg tgccgttccg
     6481 acaaagtggt atgtcgccac tgttcaggat cgtccttggc gattttgaca gcttttttga
     6541 catccgtttt tgtggccagc gcaacggaat aaacagccgg aacgtccgct gcgggttgtg
     6601 acggatcaaa aaataattgt tgtttgcgct ttgatttaac ttccgaattt ccgatatata
     6661 gcggaatttt tttgactttg gtattcgctg tcggttgcca tttcttacgg attttgtcag
     6721 cccattttcg gttttccgga agcacccagt ccgtatccgc ttcgttccgg aagattgaat
     6781 ccgcaaatgt tcccaggttt tccggaaaga cttctttgtc acggttttgc tgacgtcgtg
     6841 gttcagatga aatcttgtct ttcaactgcc atgcttcgag aaattggttt tccagaaact
     6901 tccattcaga tgaaccaacc cggatatcaa atgagtggcg caaaaagttg tccggactcg
     6961 tgttttcgtc gagccgcctt accaaatagg cgatggcgct gatgaactgg tcctgactcg
     7021 tcaccggcgt gtagagcaat aaatttgttt ctttggtttt agcttttcgt ttttcttcct
     7081 gcagagttct ccgcacatgg tcggccattc cttcgagcat ttcaaaaaca agtccctctg
     7141 tgatgccccg ctcttccgcg agaacatgtg tgtaggccaa gtcaaacaaa ttgtgtgaag
     7201 ccaaaccaaa atgcacggct gcgatatttt ccggctgaag tccgaattcc agcatacgtt
     7261 tgaaatttgc atcggtttca attttggact caaaaaccgt cagcggccaa ccgaaaattt
     7321 ccgcctcaac tgcttccatc accagattcg cgccttttac caaacgcatt tttaccggac
     7381 ttccggcgtt tttgacgcgc ttttttgccc atttgaccag ctcctgttga cgcaaaaacg
     7441 aatccggaag ataagcctgc aaaacgattc cggcctgtaa atttttgaat tccgcctgat
     7501 cgagggtacg catgaacgcg gccattgtta aatcaagatc atgataggat tccatgtcca
     7561 aatttacaaa tttagcacgc cgcgtgccgt cgctgtgcaa aaccggattt tctgaggcat
     7621 tcctgtaaag ttcggaaaga cgctcgcaaa tcaagtcgag ttcattctca aaaccgagcg
     7681 gatgcagttg cgcgacgata tttgaaattt tgatggaaat agtgtggatg tccggatttc
     7741 gcagcgactc tccgtaatgc ttcaaacggt ttaaagcttc accgtcaccc agaaccgctt
     7801 cacccagatg attgagattg acctgtacgc ctccgacttg ccgtttgttt aaatgtgctt
     7861 ttagaatttc cggctcctcc ggaagaattg tgcggcgcgt gtctgcacgg atttttcgta
     7921 atatttggga atgggagatt tgcggaagga tgtttccgaa tcgcaaaaaa atcttgagca
     7981 accactgctc gacttgcgga aaaaagcgag gaatgccgtg atgttccagc agataacgga
     8041 attgatcaac agcgcggcgt gggtttccgg aacgaaaact ctgatcaacc aaatgcatca
     8101 cgacggtttt gtcggacgga tgattgagca aacgacgcat catctgctgt tgtttcagtt
     8161 catctttggt gcgtaaagtg ttggcgcgat tttgccattt ttcggcgagg gcaattgttt
     8221 tgcgtagaat ttccgggaga tccgtttttt cctgtgaggg ggaattcatg ctctgccatt
     8281 tttcagctaa gttgaatcaa tctctctcaa aacaaagaca tggaagtaat gatcttcggt
     8341 gccgttgcac agatattcac gaccagccca gcctttgttc agaattctgg gcaaacacaa
     8401 aaatggattt tatgctcaat gcctttttca aaaagaccta aaaccgtgtc atttcgaccg
     8461 tagggagaaa tcttgcaaaa agctactaac taatattaag atttctccct acggtcgaaa
     8521 tgacatattt tggatttaaa agtttttacg agacttctat gcctaaaaac gttccccagt
     8581 tttatgatta cagggaaagt ttatgtaaat gtttgaaagg aggagcagtc tgggtcgccg
     8641 tacaaagcgt tgagtgccgg aaccggaata cgccaaatga ttcactgttt cagccgatta
     8701 acatatttcc aggcagaagg aagaaggagc accg
//
LOCUS       DTVD01000111            2192 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_21717_length_2192_cov_182.944314, whole genome
            shotgun sequence.
ACCESSION   DTVD01000111 DTVD01000000
VERSION     DTVD01000111.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21717_length_2192_cov_182.944314"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1933)
                     /locus_tag="EYO68_03950"
     CDS             complement(<1..1933)
                     /locus_tag="EYO68_03950"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIC06990.1"
                     /translation="MQEYLPQEIETKWQNYWEKQGLLDFDFASEKPKFYMLTMLPYPS
                     GDLHIGHWYAMAPSDSYARFMKMQGNEVFFPIGFDSFGLPAENAAIQNNIHPKSWTYA
                     NIERMQKQLRTMGNMFAWKNEVITCDPEYYKWSQWFFLRMMEEDLAYREHAPVDFCNH
                     CQTTLAREQVWGEDRVCERCQNPVVKKKLNQWKLRITKYADELLDFEGLDWPERVRSM
                     QQNWIGRSEGVEFALKISDSEDLSFRVFTTRPDTVFGMTFCVLAPEHILVDQITTPEQ
                     KEAVKAYKGAAERKDEIERTAEGQEKDGVFTGAFAVNPMNGESVPIWIADYVLASYGT
                     GAIMAVPGHDERDFHFARKYGLPTPVVIVAPEQLDSIPDGDDLSEPILQKENSVMVNS
                     LSFDGAVWPESFNRVADWMQKEGFGERQVNYRLHDWLISRQRMWGTPIPIIYCESCGM
                     QQVPYEELPVVLPDDAEFKPTGESPLKYHEGFLKTKCPKCGGDAERETDTMDTFICSS
                     WYYYAYVAPYWKKGETLTKSDQPWDAEKIRNMCPVDQYTGGIEHATMHLLYFRFFTKA
                     LADLGLLDFREPAKRLFNQGMILGEDHEKMSKSRGNVVKPDDLVQKYGTDTVRAYLMF
                     LGPWDAGAPWNPHGIEGLAR"
BASE COUNT          591 a          548 c          443 g          610 t
ORIGIN      
        1 aacgtgccaa cccttcaatt ccatgtggat tccagggtgc cccggcgtcc catggtccca
       61 gaaacatcag gtaagcccgc acagtatcag ttccgtattt ttgtacgaga tcatccggtt
      121 tgaccacatt gccacggctt tttgacattt tttcgtgatc ttcacccaaa atcatgcctt
      181 gattgaataa acgttttgca ggttcacgaa aatcaagcag tcctaaatcc gccagggctt
      241 tagtaaaaaa acgaaaatac aaaaggtgca tagtggcatg ttcaatgcct cccgtgtatt
      301 gatcaacagg acacatattt ctaatttttt ctgcatccca tggctgatca cttttggtaa
      361 gggtctcacc ttttttccag taaggagcaa cataagcata gtagtaccac gatgaacaga
      421 taaaagtatc catcgtgtca gtttctcttt cagcatcacc cccacattta ggacactttg
      481 ttttcagaaa accctcatga tatttcaagg gactttcgcc agtcggtttg aattcagcat
      541 catccggaag cacaacgggc aactcttcat aaggtacttg ctgcataccg cagctttcgc
      601 agtagataat cggaatcggt gtgccccaca ttctttgccg gctgatcagc cagtcgtgta
      661 aacgataatt gacctgacgt tcaccaaaac cttctttctg catccagtca gcaacacggt
      721 taaaactttc cggccaaacc gcaccgtcaa agctcaggct gttgaccatc accgaatttt
      781 ctttctgtaa aataggctcg cttaaatcgt caccatctgg gatagaatca agctgttccg
      841 gagcaacaat taccacgggc gtcggtaaac cgtattttct cgcaaaatgg aaatcccgtt
      901 catcgtgtcc gggaaccgcc atgattgcac ctgttccata ggacgcgagc acataatccg
      961 cgatccaaat cggtaccgac tcaccgttca ttggattgac cgcaaacgcg ccggtgaaga
     1021 caccgtcttt ttcctgaccc tccgcggtac gctcaatttc gtctttccgt tcagctgcac
     1081 ccttgtatgc tttgacagcc tctttctgtt caggagttgt aatttgatcg accagaatat
     1141 gttcaggcgc caaaacgcaa aacgtcatgc cgaaaacggt gtccggacgt gtggtgaaaa
     1201 cacggaagct caagtcctca gaatcggaaa ttttcagggc aaactccacg ccttcactac
     1261 gtccaatcca gttttgctgc atcgaacgta ctctctccgg ccagtccagt ccttcgaaat
     1321 caagcagctc atccgcatat ttcgtaatcc gcaatttcca ctgattcaat ttctttttta
     1381 ctaccggatt ttggcagcgc tcgcagacac ggtcttcgcc ccaaacctgt tcccgtgcaa
     1441 gagtagtctg gcaatggttg caaaaatcaa ctggcgcatg ttcacgatat gctaaatcct
     1501 cttccatcat ccgcagaaaa aaccattggc tccacttgta atattcagga tcgcaggtta
     1561 taacctcgtt tttccaggcg aacatattac ccattgtacg caactgtttt tgcatgcgtt
     1621 caatgttggc atatgtccac gattttggat ggatattatt ttgaatcgca gcattttccg
     1681 caggcagacc aaatgagtca aaaccaatag ggaaaaaaac ctcgttgccc tgcattttca
     1741 taaaacgcgc atagctgtcg gaaggcgcca tagcatacca gtgtccaatg tgcagatccc
     1801 ccgaaggata gggcagcatg gtcagcatgt aaaattttgg tttttccgaa gcaaaatcaa
     1861 agtccagcaa accttgtttt tcccagtagt tttgccattt ggtttcgatt tcttgcggaa
     1921 ggtattcctg catggttttt tctaaagggt atgaatttta gaaatgtatt gaatgactta
     1981 aacgttttat tctgcgtaga attgtggaaa aatacaatcg aaaaggtaag ataagatcaa
     2041 tcaaaggtga ggagtactga ttgtgccgga tgcgtcttta agtattggtc ccaaacgttc
     2101 tacagttgac agtcggttaa gtgcataaag atgaacccct tccacacctt cctggagcag
     2161 ttcttcaaac tgattttcaa catgatcaaa ac
//
LOCUS       DTVD01000112            2191 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_21736_length_2191_cov_213.907303, whole genome
            shotgun sequence.
ACCESSION   DTVD01000112 DTVD01000000
VERSION     DTVD01000112.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_2191_cov_213.907303"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            61..609
                     /locus_tag="EYO68_03955"
     CDS             61..609
                     /locus_tag="EYO68_03955"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIC06991.1"
                     /translation="MKRLQSECPENPLKKIILLNANASDIVPLRKWPLENFVELGKQL
                     LENSEFTVVLTGSPEEKDVCVDLALQINPDRTVNFAGKTSFKELITLYSISDLLITND
                     SGPVHFASTTEIPILALFGPGSTVDFGPMSPNAKVISLELACSPCVSVFNQKKSSCDD
                     NQCMKQISVQMVVKETQKILGK"
     gene            606..2171
                     /locus_tag="EYO68_03960"
     CDS             606..2171
                     /locus_tag="EYO68_03960"
                     /inference="COORDINATES: protein motif:HMM:PF09852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HIC06992.1"
                     /translation="MKLSVSSVIPQNPVFLWLWITLLVWWSGLAGRDFFLVPALIFVG
                     IYTYQIRNKQPSIITTKWTNSSYAKRWLISLFLVHVVLNLAITILKYYSFRWNVWDVG
                     SYSNMLYNISQGRFYSSYLGTHNWGDHFSPSMSPLALFYLWFPSTHWVTLAKTVAYLS
                     VPLLIHKICKESFQNKEQAWSVTVILGAAWMLFYAPALNSLYYEFQPSALAPPFILYA
                     FLCFQRKLWLRFWFTMIVILGFKENLGAVWIGFGCFMVLATPNKKMGFFLISGGIISI
                     YLIMFHVMPYFRNFEESWSMPVGPFQDIPGKLIYLFKILIPLGFLPLIFWRFGIIAGP
                     AIGVNLLSSGPKMYSTSYHYDDIPSTLLMVAMVLIVSSGSFNLRFWEKKKAGQWLLAG
                     WIVCVLILLPSSPMRELYASIPDKLHRVIRKELIEFDQFSKGEPIAVQTSLGPQFNRT
                     NILAITQDSNGNCAPMQRDLLVAETKYLVFAKSLNHYLIEDLEQCLKKINLSKDYEIL
                     HAYQNLHIYKKIN"
BASE COUNT          630 a          412 c          425 g          724 t
ORIGIN      
        1 ccctcccaaa ttaagaaccg ccttaaaata aacattccag aaaaagagca gcaaacttta
       61 atgaaacgtc tgcaatcaga gtgtccagaa aaccctttga aaaaaattat tttgcttaat
      121 gctaatgcca gcgacattgt tcccttgcgt aagtggcctc tagaaaattt tgtcgaactc
      181 ggcaaacaac tcttggagaa ctcggaattt actgttgtac tgaccggcag cccggaggag
      241 aaagatgtgt gtgtggattt agctctgcaa atcaatcctg acaggacagt caattttgcc
      301 ggaaaaacat ctttcaaaga actaattact ctatattcga tcagtgattt attgatcaca
      361 aatgattccg gacctgttca ttttgcttca acaactgaaa ttcctatcct ggctttattt
      421 ggtcctgggt ctactgtaga ctttggacca atgagtccga atgcgaaagt gatttcattg
      481 gaactggctt gcagcccctg cgtttcagtg ttcaatcaga aaaaatcttc atgcgatgat
      541 aatcaatgca tgaagcaaat cagcgttcaa atggtcgtta aagaaactca aaaaattctt
      601 ggaaaatgaa actgagtgtt tcttctgtaa ttcctcaaaa tcctgtattt ttgtggctat
      661 ggatcacgtt acttgtctgg tggtctgggc ttgcagggag agattttttt ttggtgcctg
      721 ctcttatatt tgtgggtatc tatacctatc agattcggaa caagcaacct tctattatta
      781 ctacaaagtg gaccaatagt tcctatgcaa aacgctggct gatttccttg tttttagtac
      841 atgtggtttt gaatctcgcg attactattt tgaaatacta ttcatttcgc tggaatgtct
      901 gggatgtggg tagttacagc aacatgcttt acaacatttc gcaggggcgt ttttactcat
      961 cctacttggg cactcacaat tggggcgatc acttctctcc ttcgatgtca ccactggctt
     1021 tattctattt atggtttcca agcactcact gggttacttt ggcaaaaaca gttgcctatt
     1081 tgagtgtgcc gctgttgatc cataaaattt gtaaagaatc ctttcaaaat aaagagcaag
     1141 catggagtgt cacggtaatt ttaggagctg catggatgct attttatgct cccgccttga
     1201 acagtcttta ttatgaattt caaccttctg ctctggctcc tccatttatt ctttatgcat
     1261 tcttgtgctt tcagcgaaaa ctatggctgc gcttttggtt tacaatgatt gtaatacttg
     1321 gcttcaagga aaatcttggt gcagtttgga ttggttttgg gtgtttcatg gttttagcaa
     1381 cacctaataa aaaaatgggt ttttttctta ttagcggagg tatcatttcc atttacctta
     1441 taatgtttca tgtcatgcca tatttccgca attttgaaga atcttggagt atgcccgttg
     1501 gaccatttca ggatattcca ggaaaattaa tatatctgtt taaaatatta attccactag
     1561 gttttttacc tttaatattc tggcgatttg gaattatcgc aggtccagca attggggtta
     1621 atttgctttc aagcggtcct aaaatgtatt cgacgagcta tcattatgac gatatcccct
     1681 cgacattact tatggtagca atggtcctca ttgtttcatc aggcagtttc aatcttaggt
     1741 tttgggagaa aaaaaaagct gggcaatggc tcttagctgg atggattgtt tgtgtactca
     1801 tcctgttacc ttcaagccca atgagggaat tatatgcctc aatcccagat aagctgcata
     1861 gggtcattcg aaaagaattg atcgaatttg atcaattttc aaaaggtgag cctatcgcgg
     1921 tacaaacttc actggggcct caattcaatc gtacaaatat tttagcaatc acacaggata
     1981 gtaatggaaa ctgtgcacca atgcaacgag atctgttagt tgcggaaaca aaatatttag
     2041 tatttgcaaa atccttaaat cactatttaa ttgaagatct ggagcaatgc ttaaaaaaaa
     2101 taaatctgtc taaggattat gaaattcttc atgcatatca aaacttacat atatataaaa
     2161 aaattaattg aactcttgga gactggtaca a
//
LOCUS       DTVD01000113            2190 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_21751_length_2190_cov_342.419672, whole genome
            shotgun sequence.
ACCESSION   DTVD01000113 DTVD01000000
VERSION     DTVD01000113.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_2190_cov_342.419672"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(416..1552)
                     /gene="sohB"
                     /locus_tag="EYO68_03965"
     CDS             complement(416..1552)
                     /gene="sohB"
                     /locus_tag="EYO68_03965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:PRK11778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HIC06993.1"
                     /translation="MNEFMEQYWSFYNAGVTIFLIAGLALIIKLVFFKRKRRKKEPHL
                     KFKHWNHRFENDFERLEKELHVAPFLPKEATKLLIKARKKEVKEEKRKDKEKSVKTIS
                     TIREKLKDGLNSEEVLKQHSNCVYVLTFIGSIMASEVEQLRDQISFLLQIAQPADEIV
                     VRLTSPGGAVPQYGLASSQLERLKQAGLRCVVCVDTVAASGGYMMAAVADKIIAAPFA
                     IIGSIGVVAGIPNFHRVLQKNEVDYHLFTAGKYKRTVTPFTEVTDEGKQKLQDDITAI
                     HEAFKRLIKEGRPDVDIEEIATGEYWLASQAKEKGLVDEIMTSDDYLGSKLDDCEVIE
                     ITTETEQNRIEKLIEGGASLFRQWTSSRIPGAGEELEDVRQRFR"
     gene            1658..>2190
                     /locus_tag="EYO68_03970"
     CDS             1658..>2190
                     /locus_tag="EYO68_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC06994.1"
                     /translation="MPKTDPNLLRTILLLSLCFALTGTTSSLTVSASPLVGFQIAGNK
                     ALATLPLSFHLIGTMLAGIPASFLMKNVGRRYGFMIGTGIGAFGAASAAGSIIYSNFY
                     LFCLSIFCLGILSGFAQLYRFAAADVATHEFKTKAVSLVMIGGIIAGFIGPTVASKAK
                     DLIPNAEFAGSYLFVIM"
BASE COUNT          578 a          465 c          424 g          723 t
ORIGIN      
        1 tgcagagtac tccgcaaatg gatcgtcacc gtgacattga acgggaagcc gcactgttcc
       61 ggaacagtcc agactatttc gacaaacgtg cggaaggatc gttaaaagcg tatgacattg
      121 ggattgatgc agctcataaa caaatcaatg gtacatagta gccgctttca tagtgaactc
      181 cccgtgcctg ctgttcaggt tacaaaggtc ttcagtattt aactcgacag tcacacgttt
      241 tatcgtgtca tcccaggcgt atataagtaa ccttataaga tattttctct tttatcagat
      301 ctctcgcatc ggtcgagatg acatgccgca atcaataata tcaatatctt ttgagaatta
      361 atggctcacg aagtaattta ttacaaattg agacaaatac gaaaaataag ctttttcacc
      421 tgaatcgctg acgaacatct tccaattctt ctcctgcacc tggaatcctg gaactggtcc
      481 attgtcgaaa cagggaggct cctccttcga ttagtttttc tatacgattt tgttctgtct
      541 ctgttgttat ttcaattact tcacagtcat caagcttgct tcccagataa tcatcgctgg
      601 tcataatttc gtcaaccagc cctttttctt ttgcctgtga agccagccag tattctccag
      661 ttgcaatttc ctcaatgtca acatccggac gtccttcttt aatcaggcgc ttaaatgcct
      721 catgaatggc tgtaatgtcg tcctgcagtt tttgtttgcc ctcgtcggta acttctgtaa
      781 aaggtgtgac tgtacgttta tattttccgg cagtaaatag gtgataatcc acctcatttt
      841 tttgcaaaac acggtgaaaa ttagggattc cggcaacgac tccaatggaa ccgataatag
      901 caaatggggc agcaatgatt ttatccgcaa cagcagccat catataacct ccactggccg
      961 ctactgtatc cacgcagact acacagcgca aaccggcctg cttcagacgt tcaagttgtg
     1021 aactcgctaa tccatattga ggaactgccc cgccgggact ggtcagacgt acaactattt
     1081 catcagcagg ttgcgctatc tgaagtagaa atgagatttg atctctaagc tgttctactt
     1141 cagaagccat tatgctgccg ataaatgtga gaacataaac acagtttgaa tgctgtttga
     1201 gtacctcctc cgaattgaga ccatctttta atttttcacg gattgttgat attgttttta
     1261 cgcttttttc tttgtctttt cttttttctt cctttacctc tttttttctg gccttaatta
     1321 acaacttagt tgcttcttta ggtaaaaaag gagcaacatg cagttctttt tccagtcgtt
     1381 caaaatcatt ttcaaagcgg tgattccaat gtttaaattt gagatggggt tcttttttgc
     1441 gacgcttgcg tttaaaaaaa actagtttga ttatcaaggc caagcctgca atgagaaaaa
     1501 ttgttacccc tgcattgtaa aatgaccagt attgctccat aaattcgttc atatagtatc
     1561 ttattcttca atgttaaact tacccagctt ggaggaagga tagaattatg ttagaatatg
     1621 cgctctttaa ttaaaacata tttattaatt tttatgcatg ccaaagactg accctaattt
     1681 attaagaact attttgctgt tgagtctttg ttttgctctg actggaacta ccagcagtct
     1741 tacagtttct gcctctccat tagtaggttt tcaaattgcc ggcaataaag cattagcaac
     1801 cctgcccctg tcatttcatt tgattggtac catgcttgcc ggaattccag cttctttcct
     1861 gatgaaaaat gtggggaggc ggtatggctt catgattgga actggaatcg gtgcatttgg
     1921 ggccgcatcc gctgctgggt caatcattta ttctaatttc tatttatttt gtctaagcat
     1981 attttgtttg ggaattttga gtggttttgc acaactatat cgctttgcag cagctgacgt
     2041 ggctactcat gaatttaaaa cgaaagcagt ttctttagtc atgatcggag gaattattgc
     2101 cggtttcatc ggaccaacag ttgcgtccaa ggcaaaagac ctgattccaa atgcagagtt
     2161 tgcaggaagc tatctgtttg tgattatgtt
//
LOCUS       DTVD01000114            2164 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_22139_length_2164_cov_283.619725, whole genome
            shotgun sequence.
ACCESSION   DTVD01000114 DTVD01000000
VERSION     DTVD01000114.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22139_length_2164_cov_283.619725"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="EYO68_03975"
     CDS             complement(<1..2021)
                     /locus_tag="EYO68_03975"
                     /inference="COORDINATES: protein motif:HMM:PF13899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIC06995.1"
                     /translation="MPEHSFTNKLINEKSPYLLQHAHNPVNWYPWGQEAFEKAKSEDK
                     LLLVSIGYATCHWCHVMERESFEDPELALYLNKHFVPVKVDREERPDVDKIHMDALHA
                     LGQQGGWPLNMFLTPEGKPVTGGTYFPPKPMYGRKSFRELLENLVFIWENEREKIYST
                     ADEITSHLQQQAVPSYNGLPELNWEVEEKAVAQFRESFDSRHGGFNQQLQNKFPPSMG
                     LMLLLRHYDRTGNAMSLEMVEKTLQKMFAGGIYDQLGGGLSRYSTDYEWLVPHFEKML
                     YDNSLFIWALIETFQVTRNPLYETAVRDVLTYVARDMTSPQGAFFSAEDADSEGVEGK
                     FYLWSKAEVTEILGAETGKLACAYWDITEKGNFESSNIPNRPMSDEEVAEQFSITTEE
                     MQIQLRAAREKLLAVRSQRIRPLLDDKVLTSWNALMISAFARAARVFDDTDFEMRAVR
                     AADFIFENLRDESGRLLRRWRDGEARYPAYLCDHAQLAAACLDLYETTYDPEWFRKAV
                     ELSQGINRLFRNDDGPYYDTGIDGETLLTRNAEGYDGVEPSGNSSVTNAFLRLRSYGL
                     SPEFYSDAQRILRSFATHLKQAGVSFSAMLGALHFSLSEPKEIIISGRRGDPETEALL
                     KEVRSEFHPNIVVAFVENGESPETEKIIPLASGRVMVNERATAYVCQNQT"
BASE COUNT          594 a          529 c          429 g          612 t
ORIGIN      
        1 gtctgatttt ggcaaacata agcggtcgca cgttcattta ccattactct tccggaagcc
       61 agcgggatga ttttttctgt ttccggactc tctccgtttt caacgaaggc cactactata
      121 ttcggatgaa attcactacg tacttccttc agtaaagctt ctgtctcagg atcaccacgt
      181 cttccggaaa tgataatctc tttcggttca gaaaggctga aatgcagggc accaagcatt
      241 gctgaaaagc ttactccggc ctgtttcaga tgggtagcaa aacttcttaa aattcgttga
      301 gcatcgctgt aaaattcagg ggacaaacca taagagcgaa gcctcaaaaa tgcatttgtc
      361 acactgctgt ttcccgaagg ttccacacca tcatagcctt ctgcattacg ggttaacagg
      421 gtttcaccat ctattccagt atcataatat ggaccgtcat cgttccggaa cagacgattg
      481 attccctgag ataattcaac agctttccgg aaccattcag gatcgtaggt ggtttcgtaa
      541 agatccagac aagcagcagc aagttgagca tgatcacaga gataagccgg ataacgagcc
      601 tctccgtcac gccatcgccg gagcaagcgt ccagactcgt cccgtagatt ttcgaaaata
      661 aagtcagccg cacgcactgc cctcatttca aaatcagtat catcaaatac ccgtgctgcc
      721 cgagcaaaag cagaaatcat caaggcattc caggatgtaa gtactttgtc atctaataga
      781 ggacgaatac gttgtgaacg aactgcaagc aacttctccc gtgcagccct gagttgaatc
      841 tgcatctctt cagtagtgat actgaattgc tctgcaactt cttcatcgga cattggacga
      901 ttcggaatgt tgctgctctc aaaatttcct ttctccgtaa tatcccaata tgcgcaggcc
      961 agctttccag tttcagcacc taaaatttcc gttacttctg cttttgacca gagataaaat
     1021 ttcccttcaa caccctcgct atccgcatct tccgcagaaa aaaatgctcc ctgcggagaa
     1081 gtcatatccc gtgcaacata agtcagaaca tcccgcaccg ctgtttcata aagtggattc
     1141 ctggtaactt gaaaagtctc tatcaaagcc cagataaaca gagaattatc ataaagcatt
     1201 ttctcaaaat gcggaaccag ccactcataa tcggtactgt agcgactcag tccaccgcca
     1261 agttgatcat aaattccccc ggcaaacatt ttttgtaaag tcttttccac catttccagt
     1321 gacattgcgt tacctgttct gtcatagtga cgaagcaaaa gcatcaatcc catgctaggc
     1381 ggaaacttgt tttgaagctg ctgattgaaa ccaccgtgcc gtgaatcaaa cgattcccga
     1441 aattgtgcaa ccgccttctc ttccacctcc caattcagtt caggtagtcc gttataagat
     1501 ggaacagctt gttgctgtaa atgagaagta atttcatcag cagtggaata aattttttcg
     1561 cgctcatttt cccaaataaa gactaagttt tccagcagct cacggaagga ttttcgccca
     1621 tacattggtt tcggaggaaa ataagtaccc cccgtaacag gctttccttc cggagtcaaa
     1681 aacatattca gaggccatcc tccctgctga ccaagggcgt gtagtgcatc catgtgtatt
     1741 ttgtccacat cagggcgctc ctctctgtct actttcactg gtacaaaatg cttgttaaga
     1801 tatagagcca attctggatc ttcaaacgac tcgcgctcca tcacgtgaca ccagtgacag
     1861 gttgcataac cgatgctgac cagcagcagc ttgtcttcac tttttgcttt ctcaaaggct
     1921 tcctggcccc aggggtacca gtttaccggg ttgtgcgcat gctgtaatag gtacgggctt
     1981 ttttcgttta tcaacttgtt ggtgaatgaa tgttcaggca tttttcaaga tggaaaagaa
     2041 attagtgttt ttttattgcc attttaagac tgtttacaaa tgatgcaata caaagttttt
     2101 ctcaggagac aagtatgatg gatgtctaat tttcactcaa gaaaaaggtt tttgtaaggt
     2161 tttt
//
LOCUS       DTVD01000115            8634 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2225_length_8634_cov_364.147919, whole genome
            shotgun sequence.
ACCESSION   DTVD01000115 DTVD01000000
VERSION     DTVD01000115.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_8634_cov_364.147919"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            166..1335
                     /locus_tag="EYO68_03980"
     CDS             166..1335
                     /locus_tag="EYO68_03980"
                     /inference="COORDINATES: protein motif:HMM:PF00482.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIC06996.1"
                     /translation="MGNFYWEGTDKQGSLHEGALESHSREAAKEHLLQEGYYRVRFWK
                     IQQSHLHRSLNNTEITEFLLQLHRLLKSGVELDDALGFAVQEQKDHVLSFLLCRIRQD
                     LRDGLSMSSALRHYQAFPQIVAKMIEVAESTGRLTEVLGMLLEFYQFQQKMILEQKRL
                     LNYPLVVFSIFIVLSLGAVIFMVPMFKRMYAGLGEDLFWLTRALVKLSDSLRFNPEAW
                     ILGTLFSGAIIVFVYRLFGWYWLLNLIPGGKRLQQSVQMLFYSRSMEIMLLTGVHLQE
                     ALALAEEMFPKLLRKRIAPVREAVDSGKSLCDAYAMVPVFSPMFVKMVALGESSGHLP
                     SSFARIGSHNQEALKKIMAWASTLIEPLLMILIALGVLGFLLSMYLPLFRMAERF"
     gene            complement(1291..1974)
                     /locus_tag="EYO68_03985"
     CDS             complement(1291..1974)
                     /locus_tag="EYO68_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HIC06997.1"
                     /translation="MLNKIRHGLADHQPLKNTQIEKRASVLIPLLESEGELFVLLTQR
                     SKQLRSHAGQVSFPGGKQDTQDANSLETALRETHEEIGLPPENVEIIGTLDQILSLHY
                     YLVTPFIGLIPEDFSPLLNTAEIESVFKVPLTFFMNGDNHWTEEFKTPIATVLAHHFD
                     YQGFDIWGLTAKLILRLLEIGLGHIPDFQVHHPDSPNWMERALDYSGEELPFDLKPLS
                     HSEQGKVHR"
     assembly_gap    2019..2118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2282..2716)
                     /locus_tag="EYO68_03990"
     CDS             complement(2282..2716)
                     /locus_tag="EYO68_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC06998.1"
                     /translation="MQKAQESEQSIQRARVSWEQSKEDLEMAKSFIKTNPDTSCLLSN
                     QAAINAFSSILQAHGHFQLPAYSSTEMLNICSSVASEVEETRPQCEVLDSALNRDLLG
                     HTRPKNIQFTPAFAKTSYEASRQIHKIIKAYWRENKARFFAP"
     gene            complement(2928..3599)
                     /gene="pyrE"
                     /locus_tag="EYO68_03995"
     CDS             complement(2928..3599)
                     /gene="pyrE"
                     /locus_tag="EYO68_03995"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIC06999.1"
                     /translation="MQSYKKEFAKFLVRAEALQFGEFTLKSGRSSPYFFNSSKFNTGP
                     LIEELGRYYASAIEENAPNCSLIFGPAYKGIPLCVSAATALSSSLNQNIGYFFNRKEK
                     KTHGDQGSLVGQLPLAKDSVVMVDDVITDGLTKIESVAMIRELCGVKFSGVLVALDRM
                     EKNSLGRDAIADFEQITGVPVWSIITIREVCDYLKNREIDGAVVLDEDIFLKIEHYLE
                     EHSVR"
     gene            3728..4684
                     /locus_tag="EYO68_04000"
     CDS             3728..4684
                     /locus_tag="EYO68_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HIC07000.1"
                     /translation="MVLNYHFQTSNQTMSKDKEKRLQKTIDALMVSYREHPEIEHIGV
                     VALPTKESIVKLVEDIQVLLFPGLIRQESFDNLNLPHIVGQQTVSIFYRLKEFIELVL
                     CWKASQEGENCEEQPEFGEQVENIAFEFLEYIPELREVLSEDVDAILQGDPASVSKRE
                     IVLAYPGLQAVSVFRIAHFLHERSVPLIPRIMTEHIHSQTGIDIHPGVSIGKGLMIDH
                     GTGIVIGETAVIKDQIRLYQGVTLGALSPQHSLANPNLKRHPTIENNVIVYSGATILG
                     DVVIGEGSIIGGNVWLTHSIQPNSRVFLKDADSALEVRVKTN"
     gene            4824..5132
                     /gene="clpS"
                     /locus_tag="EYO68_04005"
     CDS             4824..5132
                     /gene="clpS"
                     /locus_tag="EYO68_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIC07001.1"
                     /translation="MSEIEIDEQLKTKTRKKLKPPQSYRVLLHNDNYTSMEFVVYVLE
                     TVFGKSLSEATKIMIHVHENGIGICGSYTYEVAETKVEAVHGLAKQYEYPLQATMEEN
                     "
     gene            5142..7388
                     /gene="clpA"
                     /locus_tag="EYO68_04010"
     CDS             5142..7388
                     /gene="clpA"
                     /locus_tag="EYO68_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIC07002.1"
                     /translation="MFTKDLEMCLMAAQSEAIDRRHEYLCVEHILYALLHNDAGLNVI
                     RSCGGDTTLIKQDLEDFFSQQEEFDGNKLPRQTRAVQRVLHLAIMHVNSSGKASADVG
                     DVLVAIHRESQSYASYFLQKQDITRLDLLNYISHGIAKVPVEEEEGEGPEYEGLEDEE
                     EENEQTRPEQNPLNLYTQNLTLQAAENRIDPLIGRENEINRTLQILCRRRKNNPIYVG
                     DPGVGKTAVAEGLALQIFKGDIPEILEGTEVFALDIGALLAGTKFRGDFEQRIKAVLK
                     ALEKRPGSILFIDEIHTIIGAGATSGGSMDVSNLLKPALAKGEIRCIGSTTYEEYKKI
                     FEKDRALARRFQRIDITEPTLAETIKILKGLKSRYEKHHKLRYTAGSIKTAAELAEKY
                     INERYLPDKAIDVIDEAGSLVRLRPGTKRKTVAVQDIEKVVAGMARVPVERMNSTDKD
                     KLRELKKELQMQVFGQDEAIATLVQAIKRSRAGLHHPDHPIGSFLFTGPTGVGKTELA
                     KQLAFIMGINFERFDMSEYMEKHTVSRLIGAPPGYVGFDQGGMLTDAVRKNPHCVILL
                     DEIEKAHPDIFNILLQVMDHATLTDNNGREADFRNVVLVMTSNVGAKEMGSTTIGFGG
                     STPKGPSKKAIEKMFSPEFRNRLDNIITFSGLPEEVVLMVVEKMLVELEVKLQQKKVI
                     INVTDAAKSWLAKTGYDPIFGARPMGRTIQREIETVLADEVLFGKLENGGEVSIGLKK
                     DQLKFKYS"
     gene            7397..8155
                     /locus_tag="EYO68_04015"
     CDS             7397..8155
                     /locus_tag="EYO68_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIC07003.1"
                     /translation="MSYFIFDGVLECGRQYELKGEEAGHILKSRRLSVGDYFLIQDEQ
                     GLRFEVVLQNLSRNSLKFVPEKTVAVPPQSPLRLEILQALPKEKALDFILQKTTELGV
                     HRLDFFGGAHSSKVFRTSQAERQMVRWKRIALEASKQCGRQFPPEIYWHSNLETALSA
                     LPECPNSWVLSPGSADSVSWNNSTGADRAKPKEHQRVLVGPEGGFHPEEIDLALRSGM
                     RPVDLGPRILRTETAAVSAVAILQFLWGDLSQNK"
     gene            8331..>8634
                     /locus_tag="EYO68_04020"
     CDS             8331..>8634
                     /locus_tag="EYO68_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HIC07004.1"
                     /translation="MHIVVSIIFGLVLAGSVSAKESIQGHYDLVGNVPAAHSLKKVVY
                     EEFMNFGCTHCNNLHKASRNFRKIFADKVEFIDIPIVFRGQDDSPLRLYYVARKIGK"
BASE COUNT         2437 a         1717 c         2031 g         2349 t
ORIGIN      
        1 acaaatccct gaaccttatg caatcattaa accaataatt tatttatcat tcctgacctg
       61 catgaaaaat atgaaaaatc acaaaatttt ctcttgtcag aataaaagaa ctggagtata
      121 tgtcataaag ttaaattgag attataattt cttatattaa cagacgtggg aaatttttac
      181 tgggaaggga cagacaagca gggttcactg cacgaaggcg cactggagtc gcatagccgg
      241 gaggcagcca aagagcatct actgcaggaa ggatattaca gggttcgctt ttggaaaatt
      301 cagcagtcac atctgcacag gagtttaaac aatactgaga tcacagaatt tttgctgcaa
      361 ctgcaccgcc tgctcaaatc cggagttgaa ttggatgatg ctttagggtt tgcggtacag
      421 gagcaaaaag accatgtttt gagcttcctg ctctgccgga ttcggcagga tttgcgggac
      481 ggactttcaa tgagcagcgc tttgcggcac taccaggcat ttccgcagat tgtcgcaaaa
      541 atgattgagg ttgcggaaag taccggacga ctgacagagg tgctggggat gctgttggaa
      601 ttttaccagt ttcaacaaaa aatgattctg gagcaaaaac ggctgctcaa ttatcctctg
      661 gttgtattca gcattttcat agtcctgtct ctgggcgcag ttattttcat ggtgccgatg
      721 tttaagcgca tgtacgcagg tctcggcgag gacctgtttt ggttgactcg ggctctggta
      781 aaattaagtg attccctgcg ctttaatccg gaagcatgga ttttaggcac actgttttcc
      841 ggagcaataa ttgtttttgt ttacagattg tttggatggt attggctctt gaatctgatt
      901 cccggtggaa agcgtcttca gcaaagcgta cagatgctgt tctacagtcg tagtatggaa
      961 atcatgcttc tcacaggtgt tcatttgcag gaggcactgg ctttggcgga agagatgttt
     1021 ccgaagctgc tgcgcaaacg aattgctccg gtacgggaag cagttgattc cggaaagtcc
     1081 ctgtgtgatg cttatgcaat ggttccggtt ttttcgccaa tgtttgtgaa aatggttgct
     1141 ctcggagaat caagtggaca ccttccttca agttttgcgc gaatcggctc acacaatcag
     1201 gaagcactga aaaaaataat ggcctgggcc agtacgctga ttgaaccgct gttgatgatt
     1261 ttgatcgctt tgggcgtgct tggctttctc ttatcgatgt accttcccct gttcagaatg
     1321 gctgagcggt tttaaatcaa acggcagctc ttctcccgaa taatccagcg ccctttccat
     1381 ccagtttggc gagtccggat gatgtacttg aaaatccggg atgtgaccga gaccgatttc
     1441 cagcagacgc agaatcagtt tagcagtcag gccccaaata tcaaaaccct gataatcaaa
     1501 atgatgtgcc agtacagttg caatcggtgt tttgaattcc tcggtccagt gattgtcgcc
     1561 gttcatgaaa aaggtgaggg gcaccttgaa gactgattca atttcagctg tatttagaag
     1621 aggcgaaaaa tcttcgggaa tgagaccgat aaagggtgtg accagataat aatgcagtga
     1681 gagaatctgg tccagtgtgc cgataatttc tacgttttcc ggaggcagac ctatttcttc
     1741 atgggtttca cggagtgcag tttccagtga atttgcatcc tgtgtgtcct gcttccctcc
     1801 cggaaaagaa acctgtcctg catgcgagcg cagttgctta gaacgctgag tcagcaggac
     1861 aaatagttca ccctcagatt ccagcaaagg tataagcaca gaggcacgct tttcgatttg
     1921 tgtattttta agcggctgat ggtcggcaag accgtgtctt attttattta gcatagtgcc
     1981 tgaaaaataa agattattag ctatcagtaa tcagtaatnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnat cagtaatcag taattggcaa tcagttttca gagtacaagc
     2161 cagattttta taccgattgc tgacagctaa aaactgacgg ctatttttct gaacctgaac
     2221 agagtgttat tccaaatata aacaactgga gtgctgacac agtttcactg ctaacaacca
     2281 atcatggagc aaaaaaacgc gctttgttct ccctccagta ggctttgata attttgtgaa
     2341 tttgcctgct tgcttcgtac gaagttttgg cgaatgccgg tgtgaactgg atattttttg
     2401 gacgggtgtg tcccagtaaa tcccgattca gggcactgtc aagtacctca cactgcggcc
     2461 gtgtttcctc gacttccgat gcaacagaac tgcatatgtt gagcatttca gttgaagagt
     2521 aagccggaag ctgaaaatga ccatgtgcct gcaaaattga actgaaggca ttgatcgcag
     2581 cctggttgct cagcagacag ctggtatccg gatttgtttt gataaaggat ttggccattt
     2641 ccaggtcttc tttggattgc tcccaagaaa cccgggcacg ctgaatgctt tgttcagact
     2701 cctgtgcttt ctgcatattt gttttaaata ctaaacagtt acgagtttta tcatgtcatc
     2761 ccgaacgtac tgtgagggat catataagga aaccgcactt ttttcgatct ctctcttcgg
     2821 tcgagatgac actctgcaat caataatatc agtatcatgc gagaattcat tactcaatag
     2881 aaacaaaggt aaaatagaat gataagtatt ttactttccg gaccttgcta acggacactg
     2941 tgttcttcaa gataatgctc tatttttaga aatatatcct catccaaaac aacggcaccg
     3001 tcgatttcac gatttttcag ataatcacaa acttcacgga tagtgatgat agaccagact
     3061 ggaacgccgg ttatttgttc aaaatccgca atggcatcac gtcccagaga atttttttcc
     3121 atccggtcaa gtgccactaa aaccccggaa aacttaaccc cgcaaagctc ccgaatcatg
     3181 gcaacagact cgattttagt cagtccatca gtgatcacat catcaaccat aaccaccgaa
     3241 tccttcgcca acggcagctg accaacaagt gatccctggt caccatgtgt ctttttctct
     3301 tttcgattaa agaaatatcc tatattctgg ttaagactgc tcgacaaagc tgtggccgca
     3361 gaaacgcaca aaggaattcc tttgtaagcc ggaccgaaga ttaaactgca gtttggagca
     3421 ttttcctcaa ttgccgaagc atagtatcga cccagctctt caatcagggg gccggtgttg
     3481 aattttgagg aattgaaaaa gtaaggtgag ctgcgcccgc ttttgagcgt gaactcccca
     3541 aactgcaggg cttctgcacg tacaagaaat ttggcaaatt cttttttgta gctttgcatt
     3601 ctaaaccgga gattatttta gatactttcg caaaaagttc caaaaccgtg tcatttttga
     3661 gttatatttt acagcataac agttagtttc attcaactca attccggaac attatttctt
     3721 caacgaaatg gttctgaatt atcattttca aaccagtaac caaacgatgt caaaagataa
     3781 ggaaaaaagg ctgcaaaaaa caattgatgc cctgatggtc tcttaccggg aacatccgga
     3841 aatagaacat atcggcgtgg ttgctttgcc gactaaggaa agtattgtta aactggttga
     3901 agatattcag gtactgctgt ttcccggttt aatccggcag gagtcttttg ataatctcaa
     3961 tttacctcat attgttggac agcaaacggt ctcgattttc taccgcctca aggaattcat
     4021 agagctggtt ctttgctgga aggcttcgca ggaaggagaa aactgtgagg aacagcctga
     4081 atttggagaa caggttgaaa acattgcttt cgaatttctg gaatacattc cggaactgcg
     4141 tgaagttctc tctgaggatg ttgacgcaat tcttcaaggc gatcctgcat cggtcagcaa
     4201 gcgtgagatc gtgctggcct accctggtct tcaggcagta agtgtcttta gaattgcgca
     4261 ttttttgcac gaaaggagcg tgcctttgat tccgagaatc atgactgaac acattcattc
     4321 acaaaccgga attgatattc atccgggtgt ttcaattggg aaaggcctga tgattgatca
     4381 cggcaccggt atcgtgattg gggaaactgc agtaatcaag gatcagatcc ggctttatca
     4441 gggtgttact ttaggagcac tcagtccgca gcattctctg gctaacccga atttaaagcg
     4501 tcatccaact atcgaaaaca acgtgatcgt gtattccggc gcaacaattt taggagatgt
     4561 ggtgattgga gaaggctcaa ttattggtgg aaatgtctgg cttacacaca gtattcagcc
     4621 caatagtcgg gtttttttaa aggatgctga ctccgcactg gaagtaagag tgaagacaaa
     4681 ttagtgaaaa atattaaaaa acagattgac ttaactaaaa aatgagttaa agtgcaatta
     4741 tctacagaaa aacgatttgt ttgaaatttt catgcagaat tggattagtt tgaaaatcag
     4801 ggctgggttt ccgggaaaaa ataatgagtg aaattgaaat tgatgaacag ctgaagacta
     4861 aaacgcgaaa gaagctgaag ccgcctcaaa gctacagagt tctgctacat aatgataatt
     4921 atacctcgat ggaatttgtg gtgtatgtac tggaaacagt gtttggaaaa agtttaagtg
     4981 aggcaacaaa gatcatgatt catgttcatg aaaacggaat tggaatttgc ggttcttaca
     5041 cttatgaggt tgcagaaacg aaagtggaag cggtacatgg gttggcgaaa caatatgaat
     5101 atcctctaca ggcaacaatg gaagaaaact gagttgagtt tatgtttact aaagatttag
     5161 aaatgtgcct gatggcagct caaagtgaag ctattgaccg gcgacatgaa tatttgtgtg
     5221 ttgaacacat actctatgct ttgctgcaca atgatgcggg acttaatgtt attcgaagtt
     5281 gtggaggaga cacaaccctc atcaagcagg atttggagga cttcttttct cagcaggaag
     5341 aatttgatgg aaacaagttg ccgcggcaga cacgtgccgt tcagcgtgtg ctgcatttag
     5401 caatcatgca tgtcaacagt tccggaaaag cttctgccga tgtcggggat gtgctggttg
     5461 caattcaccg tgaatctcag tcctatgcgt cttatttttt acagaaacag gatatcaccc
     5521 gtctggattt gctgaattat atttcacacg gcattgccaa agtaccggtg gaagaagagg
     5581 agggagaagg acctgaatat gaaggactgg aagacgagga ggaagaaaat gaacagacac
     5641 gtcccgaaca gaatccactg aacctttaca cccaaaactt gactctgcag gcagcagaaa
     5701 accggattga tcctctgatt ggacgcgaaa atgagatcaa ccgaactctc cagattttgt
     5761 gcagacgtcg caaaaacaac ccgatttatg tgggagatcc tggagttgga aaaaccgctg
     5821 ttgccgaagg acttgccctg cagattttca aaggtgacat tccggaaata ctggaaggaa
     5881 cagaagtatt tgctctcgat attggcgctt tactggcagg aaccaagttt cgtggagatt
     5941 ttgaacaacg tataaaagct gttctgaaag cacttgaaaa gcgtccgggt tcgattttat
     6001 tcatagatga aattcacaca attattggag caggcgcaac cagcggggga tctatggatg
     6061 tttcaaatct gctgaaacct gctttggcaa aaggagaaat ccgttgtatc ggttccacga
     6121 cttatgaaga atacaaaaag atttttgaaa aggaccgtgc gcttgcacgc cgcttccaga
     6181 ggattgacat cacagaacct acactggcgg aaacaatcaa gattctcaaa ggtttgaagt
     6241 cacgatatga aaaacatcat aagttgcgtt acacagccgg ttccataaaa actgctgcag
     6301 agcttgctga aaaatacatt aacgagcgtt atttgccgga caaggcaatt gatgtgattg
     6361 atgaggccgg ctcgctggtg cggttgcgtc ctggtacaaa acgcaaaaca gttgctgtac
     6421 aggatataga aaaggtggtt gcaggaatgg cgcgtgttcc tgtggaacgg atgaattcta
     6481 ccgataagga taaactgcgg gaactgaaaa aagaattgca gatgcaggtt tttggtcagg
     6541 atgaagcaat agcaacttta gtacaggcca tcaagcgctc ccgtgcagga ctacatcatc
     6601 cggatcatcc aattggctct tttctgttca ccggacccac cggtgtagga aaaacagaac
     6661 tggcaaagca actggccttt atcatgggaa ttaattttga acgctttgac atgagtgaat
     6721 acatggaaaa gcataccgtt tcccgcctga tcggcgcgcc tcccggatat gtcgggtttg
     6781 atcagggtgg tatgctgacg gatgcagtgc gcaaaaatcc gcattgtgtg atcctgctgg
     6841 atgaaatcga aaaagcgcat ccggatattt tcaatattct gctgcaggtt atggatcatg
     6901 caaccctgac tgacaacaat ggccgtgaag cggatttccg caatgttgtg ctggttatga
     6961 cttccaatgt gggtgccaag gaaatgggtt cgaccaccat tggatttggc ggaagtaccc
     7021 ctaaaggtcc ttccaaaaaa gctattgaaa aaatgttcag tccggaattc cgcaaccgtc
     7081 tggacaacat cattacattt tcaggtcttc ctgaagaggt tgttttgatg gtcgtagaaa
     7141 aaatgctggt ggagttggaa gtcaaacttc agcagaaaaa agtaattatc aatgtaacgg
     7201 atgcagcaaa aagctggtta gccaagacag gttatgatcc aatattcggt gcacgtccga
     7261 tgggacgcac gattcagcgt gaaattgaaa ccgtacttgc agatgaggtt ttgtttggaa
     7321 agctggaaaa tggaggagag gtcagcattg gtctgaaaaa agaccaactg aagtttaagt
     7381 attcctgatc agaaaaatgt cgtattttat ttttgacgga gtgcttgaat gcggcaggca
     7441 atatgaacta aagggtgaag aagcagggca tattctgaaa tcacgtcgtc tgagtgttgg
     7501 ggattatttt ctcattcagg atgaacaggg ccttcgtttt gaagtagttt tgcaaaacct
     7561 ttcccggaat tctctcaagt ttgttccgga aaaaacggtt gcggttccgc ctcagtcacc
     7621 tcttcggttg gaaattttgc aggctttgcc caaagaaaaa gcattggatt ttattctgca
     7681 aaaaacgact gaacttggtg ttcatcgttt ggattttttt ggcggcgctc attcttcaaa
     7741 agtattccgt acttcacagg ctgagcgtca aatggtacgc tggaagcgta ttgccttgga
     7801 ggcttccaaa cagtgcggac ggcaattccc tcccgaaata tattggcatt cgaatctgga
     7861 gacagctttg tctgcacttc cggaatgtcc aaattcatgg gtactttccc cgggatcggc
     7921 tgattctgtt tcctggaata atagcaccgg tgcagaccga gcaaaaccaa aggagcacca
     7981 gcgggtgttg gttggtccgg aaggagggtt tcatccggag gaaattgatt tggcgctccg
     8041 ttccggaatg cgtccggtgg atcttggccc aaggattctc agaacggaaa ctgccgcagt
     8101 gtctgctgtg gcgattctgc agtttttgtg gggagacctt tcacaaaata aataaatagg
     8161 gtttgctgaa aaagtaattt atttgtcatc actgacttgt tccgggaacc agatgcccgc
     8221 taaggttgag aataagattt tctgtaagcc ccctggattc ctggtttgcg ttgtgcttca
     8281 cccggaaaga caaaaaagaa acatcatcaa caaaaaattg gagtaaagaa atgcatatag
     8341 tagtatcaat aatctttggg ttggtcctgg ctggttctgt ttcggccaaa gaatcgatcc
     8401 aggggcacta tgatctagtt gggaatgtac ctgctgcaca tagtcttaaa aaagttgttt
     8461 atgaggagtt tatgaatttc ggctgcactc actgcaataa tcttcacaaa gcctcgagaa
     8521 acttccgcaa aatatttgca gacaaagtag aatttatcga cattccgatt gtcttccgtg
     8581 gacaggatga ttcccctttg cgtctctact atgtggctag aaaaattggc aaag
//
LOCUS       DTVD01000116            2152 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_22329_length_2152_cov_154.243681, whole genome
            shotgun sequence.
ACCESSION   DTVD01000116 DTVD01000000
VERSION     DTVD01000116.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22329_length_2152_cov_154.243681"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            58..432
                     /locus_tag="EYO68_04025"
     CDS             58..432
                     /locus_tag="EYO68_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC07005.1"
                     /translation="MRVIGVTMENGAAMKLSLEAGKPVPVIEEPSLADSLGGGIGLDN
                     QYTFQLVRDLVDETLLVSEDEIAQAMRYIFETQRLVVEGGAAVGVAALLAKKIEVKGK
                     NVALVYSGNNVSSETVRKVLEG"
     gene            531..1835
                     /locus_tag="EYO68_04030"
     CDS             531..1835
                     /locus_tag="EYO68_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC07006.1"
                     /translation="MTKNYDAIIIGAGIIGAAIGYELSKKGLHTLNVEMLPAAGYGST
                     SNSCAIIRLYYSTLDGSAMAYDGYYYWREWADYLEAPKEEQLAKFHECGTLVMKTKLN
                     DGLRKQLVFMDALNICYEHRSNAQILENYPFYDLSSFAPAKTLDDPNFGEPTGGEIDG
                     AIFSPKGGYISDPQFATRNIQLAAERAGAAFLFNSRVKEIPTKNGSVEGVLLSDGTRI
                     NAPVVINVAGPHSSQINAMVGADADMKISTRALRQEVAHVPVPKGIDYSAVACVTSDS
                     DAAVYTRPEVGNNILIGSEDPPCDSHIFVDPDNYDLNFSNQWTVQAQRLGLRFPDLGI
                     PGKLKGCVDLYDVTEDWMPIYDSSCIEGYFMACGTSGNQFKNAPVVGKVMAELIEYCE
                     SGNDHDGEPMGYQLENIDHEISLATFSRLREINQNSTFSVLG"
     gene            1859..>2152
                     /locus_tag="EYO68_04035"
     CDS             1859..>2152
                     /locus_tag="EYO68_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006741389.1"
                     /note="cyclodeaminase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIC07007.1"
                     /translation="MKIFSRAELEHAVKLDTVALGVIRNGFVALAEDRVVMPPILSMD
                     VAENNGEVDVKTAYIEGVPQFAIKVSPGFFDNPKLGLPSLNGLMILFSSQTGLI"
BASE COUNT          576 a          443 c          573 g          560 t
ORIGIN      
        1 ggtttggaat tgctggaaga ttttccggaa ctcgattgta cagtagttgg gctttaaatt
       61 cgggtgatcg gagtgactat ggaaaacggt gcagccatga aactcagctt ggaagccggg
      121 aaaccggttc cggttattga ggagccaagt ctggccgata gtttaggcgg cggaattggt
      181 ttggataatc aatatacttt ccagttagta cgggatttgg tggatgaaac tttactcgtt
      241 agcgaagatg aaattgcgca agccatgcgc tatatttttg aaacacaacg gctcgtcgtc
      301 gaaggtggtg ccgcagttgg agtagcagca ttgttggcca agaagatcga agtaaaaggc
      361 aaaaatgtgg ctttggttta cagcggaaat aatgtttctt cggaaaccgt gcggaaggtg
      421 ttggaaggct aaaacggaga attgtgaaag gcaaagcatt gattattgtt gtggcaaaga
      481 aggtaaagca ggagaaattg attacataaa atttaaatca ggtgccaaca gtgactaaaa
      541 attatgacgc aattatcatc ggggcaggaa tcattggtgc cgctataggc tacgaactgt
      601 caaaaaaggg actgcacacg ctgaacgttg agatgcttcc tgctgccgga tatggatcaa
      661 cgtctaattc gtgtgccata atcaggcttt actattcaac gctggacggc agcgcaatgg
      721 cctacgacgg ttattattac tggcgagagt gggcggacta tttggaggcc ccaaaagaag
      781 aacagttggc gaagttccac gaatgtggaa ctttggtcat gaagacaaaa ctcaatgatg
      841 ggcttcgcaa gcaactcgtt ttcatggatg ctcttaacat ttgctatgag caccgttcta
      901 acgcacaaat cctggaaaat tatccttttt atgatctttc ttcctttgca ccggctaaga
      961 ctctggatga tccaaatttc ggtgagccga ccggcggtga aatcgatgga gctatttttt
     1021 ccccaaaagg aggctacatt tccgacccgc aattcgcaac ccgaaatatt cagcttgccg
     1081 cggaacgtgc gggggctgcg tttctattca acagccgcgt aaaagaaatt cccacaaaaa
     1141 acggtagcgt tgaaggggtg cttctctcag acggaacacg aatcaatgct ccagtcgtga
     1201 tcaatgtagc aggaccgcat tccagtcaga tcaatgcgat ggttggcgcc gacgcggata
     1261 tgaaaatttc gacacgtgcg cttcgacagg aagtagccca tgtgccggtt cccaagggca
     1321 tcgactattc ggctgtcgcc tgcgtcacct ctgacagtga tgcggccgtt tatacccgtc
     1381 ccgaagttgg caacaacatt ctgatcggca gtgaagaccc accctgtgac tctcacattt
     1441 tcgttgaccc ggataattat gatttgaatt tctctaatca gtggacagta caggcacaga
     1501 gattgggtct tcgttttccg gatctcggaa ttccgggcaa actcaagggc tgtgttgatc
     1561 tttatgatgt gaccgaggac tggatgccga tttatgacag ctcatgtatc gaaggttatt
     1621 tcatggcctg cggtaccagc ggcaatcagt tcaagaacgc accggtagtt ggaaaggtga
     1681 tggcggagtt gattgagtac tgcgaatccg gaaatgatca cgacggggag ccaatgggtt
     1741 atcaacttga gaacattgat catgagatca gcctcgcaac tttttcgcgt ctccgtgaaa
     1801 tcaatcaaaa cagtacattt tctgtactgg gctaagaaga tatttgtgga ataattacat
     1861 gaaaatcttt tcccgcgccg aattggaaca tgccgtcaaa ctggacacgg tagcgctggg
     1921 cgtaattcga aacggttttg tcgccctggc ggaagatcgt gtggtgatgc cgccgatttt
     1981 gagcatggat gttgccgaaa acaatggcga agttgatgtg aaaacggcgt acattgaagg
     2041 tgttccgcaa tttgccatta aggtcagtcc gggctttttt gataatccaa aattgggttt
     2101 gccgagtctg aacggtttga tgattctgtt cagttcgcaa accggattga tt
//
LOCUS       DTVD01000117            8611 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2235_length_8611_cov_428.288102, whole genome
            shotgun sequence.
ACCESSION   DTVD01000117 DTVD01000000
VERSION     DTVD01000117.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_8611_cov_428.288102"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            258..1823
                     /locus_tag="EYO68_04040"
     CDS             258..1823
                     /locus_tag="EYO68_04040"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC07008.1"
                     /translation="MNRSELSELSDKSFDVIVVGAGINGAGAAQQLVSDGYRVLIIDK
                     GDFAGGATSKSSRILHCGLRHLAPGKSIWDFVFHPSKFYLACHNARKSMIARSDISSS
                     MKELVRPFRFCFPIFKDDPYRSWQVDFAFKLLNLLGPHDNSLEYQRFSGENLQKIPFK
                     NWFRDSKQLQSVSVFKDYQFISAARLVVDTIRDAAEMGATVRNYTLMQQSKQLAEGWQ
                     LTLRDVLTSEEVMVKTKLILNVTGPWGNRFNQTLKSVIPDKVIGLKGAHIVVKLPEEF
                     SEWGIMIINRVNEPMYFLPWHGMHYIGLNRTPYDGNLDEVKATEDEAEWLLGEVNYVF
                     PQLSLQRKDILYSYAGIQPVTFDASDPQGNREVVVHDLASEGLHNVLMLTGGPIMTYR
                     LIAKQLLKEVSKRCVPTLAKQHPSSGSSEVTKLLRNSRSASVEMNISDEILKKIIVEE
                     QPVSLADILLRRTGIGWDIDQGKSAVPGVATVMAELCGWDEQRKEKEMQLFHQHIKEI
                     YQVQKYWGDSVCD"
     gene            2135..3172
                     /locus_tag="EYO68_04045"
     CDS             2135..3172
                     /locus_tag="EYO68_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC07009.1"
                     /translation="MKNLVYTMTQLSRGVLGVVLGLMLAIPVSAENLTVYTAVEAEDL
                     KRYAAEFNKDHPDIKIRWVRDSTGIVTAKLLAEKNNPRADIIWGLAATSLMLLQSEGM
                     LQPYAPKGLGKLDSKFRDSANPPSWTGMDAWIAAVCVNTVEAKKNNLPTPTSWMDLTD
                     PVYKGHLVMPNPNSSGTGFLDVSSWLQIYGERGGWQFMDNLHKNIAWYTHSGSKPCKQ
                     AARGETPIGISFAFRGAKSKAAGAPLEIVLPKEGVGWDMEATAIVKGTKKLAAAQTLA
                     DWSITKKANVLYNKGYAVVAYPGVAKPVKYFPAGILEAMIDNDFEFAAVNRKRILAQW
                     QKRYDGKSEAK"
     gene            3240..4322
                     /locus_tag="EYO68_04050"
     CDS             3240..4322
                     /locus_tag="EYO68_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HIC07010.1"
                     /translation="MPESKQNFLRIDNLTKHFGEFVAVQELSLEINDGEFICFLGPSG
                     CGKTTLLRAIAGLDPQSSGSITQAGKDISNLPPSQRDFGIVFQSYALFPNLTVFQNVA
                     YGLENQKIPKPEIRKQVNDLLELVGMPEQTGKYPAQLSGGQQQRVALARALATSPGLL
                     LLDEPLSALDARVRATLRLEIRSLHERLGVTTILVTHDQEEALSMADRIVVMNEGRIE
                     QVGTPEEIYSAPASPFVADFIGVMNFLQGEVTNSGKVLIGENTLDCETANIPEGSKVR
                     VTVRPEDILCSADDKNAANSFSVKVESVEFLGSFCRLILSGQEGFGKLMTDLSTQQVH
                     ELNVTPETTLTAVFPARSLKVFPESL"
     gene            4319..6043
                     /locus_tag="EYO68_04055"
     CDS             4319..6043
                     /locus_tag="EYO68_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HIC07011.1"
                     /translation="MSTADSIPASTVPARGASSVRIKLSRDDYALRTGLVVVISLLAI
                     LVVLPLYSLLSKSLEDMDGNFVGLQNFQEYFQTPALLTSITNSLSVAFFSAIIVLVLA
                     FIYAYALTRTKMPFRLFFRGVALIPILAPSLLAAISLIYWFGTQGVLKSWLFGASIYG
                     PIGVIMASCFWVFPHALMILITALSTADARLYEAAEALRTPKWRIFWTVTLPGAKYGI
                     LSTGFIVFTLVITDFGVPKVIGGNFNVLATDVYKQVVGQQNFEMGAVVSLVLLLPAVA
                     AFIADRWVQSKQVALLSARAVPFQPKSEPLRDWLLFGFCATIAFIFLAMLGMAAYASF
                     VTFWPYNLTLSLNNYDFDLMDGGGWEAYFNSIRMAIYCAVFGSGLIFFGSYLVEKARG
                     VSWLRTILHLIAMMPLAIPGLVLGIAYIFFFNHPDNPLGFLYGTMGILVISTIVHFYT
                     VSHLTSLTALKQLDPEFETVSLSLKTPVWRTFGKVTLPVCLPALLDVASYLFLNAMTT
                     VAAVVFLYSPETMLASIAVLNMDDAGDIAPAAAMALMIVYTSAAFRLLHALLTRYLKR
                     RTQVWRNS"
     gene            complement(6198..6623)
                     /locus_tag="EYO68_04060"
     CDS             complement(6198..6623)
                     /locus_tag="EYO68_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidylprolyl isomerase"
                     /protein_id="HIC07012.1"
                     /translation="MKVGTSAVTQQQAAKTNKRPQLALDAAFKHTRSGIQVYVERPGH
                     GDKTKDGSHIKVHYEGWLAEDYKMFDSSRAKRRPFEFDLGKGSVISGWDEALKGVREG
                     TKLQIKIPAKLAYGQQGVSSMGIPPNADLIFKVEVLKVT"
     gene            complement(6620..7873)
                     /locus_tag="EYO68_04065"
     CDS             complement(6620..7873)
                     /locus_tag="EYO68_04065"
                     /inference="COORDINATES: protein motif:HMM:PF03968.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07013.1"
                     /translation="MLKRKIKNIFSVFLLFFSIATTEPAWNYRQASLAAPASGYEITR
                     NTLDHLQREGFSKALTEMLSPILNTHYLNLKDFLEALPIQLTKKESDLLMRYASTQQI
                     KIQADEFSSDLNENAAVFRGNVKGVIPRENIELTTAKLRLVSGKGKNFEKLIGEGGVS
                     VKQWDREVRSDFAIYTQIDLEDQTPDTDSAETKSIETPQQTLRLQGNVIMKASQGSVI
                     SNTVFVDILRQHATLEGKDTSKEGRIKVEANLSELETNRKEQSSKIKTQEEETENQEK
                     TEQKKVLLLASRGVLDNIKHLAMFEGDVEMERTPDNLYIHAGKITLRLNELQELQSIR
                     AEQGVCFEQPGRVAKADQANFDEIKQTILLEGSAEVQSGKYHLQGNTINLYLDVNKGI
                     AQGDNKAPIQMTILGEQAPAIFACR"
     gene            complement(7866..>8611)
                     /gene="pyrG"
                     /locus_tag="EYO68_04070"
     CDS             complement(7866..>8611)
                     /gene="pyrG"
                     /locus_tag="EYO68_04070"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509607.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HIC07014.1"
                     /translation="SYESLNEALLHGGIANRLKVNLHYVDSELLTEKNVLSQLEKMDG
                     VLVAPGFGERGTEGKIAAIKIARTKNIPFFGICLGMQMAIVEFARNVVGIEGANSGES
                     RPDHPENVIDLMPEQEGQEETGGTMRLGSQTTILEKGTRIEKIYNKTTISERHRHRYE
                     VMNYFVPRFEEAGLTVSGRHPERNLVETIELAEHPWFIGCQYHPELQSKPLKPHPLFT
                     SFIEASYLSRQSCSDHQATSKKIADTSNA"
BASE COUNT         2207 a         1922 c         1926 g         2556 t
ORIGIN      
        1 ctgactttgt cattgaaaat ccgttcagtc caggaactcc ggaaacattt gaaagattta
       61 gcaaatgcgg ttccggatat tcaggaggaa atttcggaag atgtatggaa ggagttgttt
      121 gctttgctgc aggaagatta attatactat tccggaaaat tttgattgtg atttcaactg
      181 tttatttaag tttaaatctt ttaaacagac aataaaaaac atactaattc ttttaaatat
      241 cagtagagat ttgaaaaatg aatcgtagtg agttatcaga actttcagac aaatcatttg
      301 acgtaatagt tgttggcgca ggaataaatg gtgccggtgc ggcacagcag ctggtttccg
      361 atgggtacag agttttgatc attgacaaag gagattttgc cggtggagca accagcaaat
      421 ccagccgtat tctgcattgc ggtttacggc acttggcgcc tgggaaatcc atttgggatt
      481 ttgttttcca tccatctaaa ttctatcttg cctgtcacaa tgcaagaaaa tcgatgatcg
      541 cgcgaagtga catttcttca tcaatgaagg agttggtgag acctttccga ttttgttttc
      601 ctatttttaa agatgatcct tatcgttcgt ggcaagtcga ttttgctttt aaattgttga
      661 atctgttagg accacatgac aattcgctgg agtaccaaag attctcaggg gaaaatttgc
      721 aaaaaattcc attcaaaaac tggtttcgag attcaaaaca gttgcagagt gtttcagttt
      781 ttaaggatta tcaatttata tcggctgcaa ggttggtggt tgatacgatt cgtgacgccg
      841 cagaaatggg agcaactgtc cgaaattaca ccttgatgca gcaatcaaaa caattagctg
      901 aaggttggca gctaaccctc agagatgtct taacttctga agaagtaatg gtaaaaacta
      961 agttgatcct gaatgtcaca ggaccctggg ggaatcggtt caaccaaaca ctgaaatccg
     1021 tcatacctga caaagtgatt ggactgaaag gtgcccacat cgtcgtcaaa cttcctgaag
     1081 aattttctga atggggaata atgatcatca accgtgtaaa cgaaccaatg tacttcctgc
     1141 cctggcatgg aatgcactat atcgggttaa acagaacccc atatgatgga aatcttgatg
     1201 aagtaaaggc gacagaagac gaagcggagt ggttgcttgg agaggtaaat tatgtatttc
     1261 cacagctaag tcttcagcga aaggatattc tgtattcgta tgcaggtatt cagccggtaa
     1321 catttgacgc aagtgatccg caaggtaatc gtgaggtagt agtacatgat ttagcatcag
     1381 aggggcttca taatgtttta atgctcacag gcggtcccat catgacatac cgtcttattg
     1441 caaaacaatt attgaaggaa gtatcaaaac gatgtgttcc aacattagca aaacagcacc
     1501 cttcatccgg ttcaagcgaa gtgaccaaac tccttcgcaa tagccgatcg gcatcagttg
     1561 agatgaacat ttcagatgaa attttaaaga aaataattgt tgaagaacaa cctgtttctc
     1621 ttgctgatat tttactacgc aggacgggaa ttggctggga cattgatcaa ggcaaatctg
     1681 cagttcctgg agtggcaact gtgatggcag aactatgcgg ttgggatgaa caacgaaaag
     1741 aaaaagagat gcagttgttt catcaacata ttaaagagat ataccaagtg caaaaatatt
     1801 ggggggactc agtttgtgat tagtgtgtgg tagaagaagg agtcaaactt tgcagtccat
     1861 tattgatagg cactgataat gttcacctca gcttctagca atgctttttt tattttgtct
     1921 ggcggaggct tttcacacat gctatagtgt ctttaggaaa atacatgtgt ttgtattttc
     1981 agccttgctg agacgcagat cactcgtagt gattcttgcc agcagggttt ccctcggatc
     2041 agaaaataaa agattccgag atatcaacag ttgtcgaatt ctgttttcag agaataacgg
     2101 cagctggttt tttaacccat gtttagagag accaatgaaa aatctcgttt atacaatgac
     2161 ccaacttagc cggggtgttc tcggagtagt tttgggcctg atgcttgcca ttccggtaag
     2221 tgcagaaaat ctaactgttt atacagcagt ggaagcagaa gacctcaaac gctatgctgc
     2281 tgagttcaac aaagatcatc cggatatcaa aatccgttgg gtgcgggatt ccactgggat
     2341 tgtcactgca aaactgcttg cggaaaagaa caaccccaga gccgatatta tttggggcct
     2401 ggccgcaacc agtttgatgt tactccaatc agaaggaatg ctgcaacctt acgcgcctaa
     2461 aggtttgggt aagcttgatt cgaagttccg tgattcggct aatccaccaa gctggaccgg
     2521 aatggatgcg tggattgccg ctgtttgtgt aaacacggta gaagcaaaaa agaacaattt
     2581 acccactcca acctcatgga tggatctcac cgatcctgtc tataaaggac atcttgtcat
     2641 gccaaatcca aattcatccg gtacgggatt tcttgatgtt tcctcctggc ttcaaatata
     2701 cggagagcgc ggtggatggc agttcatgga caacctgcat aaaaacatcg cctggtacac
     2761 ccattccggc tcaaagccct gcaaacaggc agctagaggt gaaactccga ttggaatttc
     2821 ctttgcattc cgtggtgcaa aatccaaagc cgccggtgcc cctctggaaa tcgttttacc
     2881 aaaagaagga gttggctggg acatggaagc aactgcaatc gtgaaaggga ccaaaaaact
     2941 tgctgccgca caaactctgg cagattggtc gatcacgaaa aaagcaaatg tgctttacaa
     3001 taaaggctat gcagttgtag cctatcccgg agttgccaag ccggtcaagt atttccctgc
     3061 cggaatactt gaggccatga ttgacaatga ctttgaattt gcagcagtga accgcaagcg
     3121 aattcttgct cagtggcaaa aacgttacga cggaaagtct gaagctaagt aattctggtt
     3181 tcaaatttcc tttttcccgg gtcggatttt ctttcggccc ggatcttcta caccttacca
     3241 tgcctgaatc aaaacaaaat tttcttcgca tagataatct cactaaacat tttggcgagt
     3301 ttgtcgcagt tcaggaactt tcactggaaa ttaacgacgg cgaattcatc tgttttctcg
     3361 gaccgtccgg atgtggaaaa accacgcttt tgagagccat cgccggactt gatccgcaat
     3421 cttccggaag cattacacaa gccggaaagg acatttccaa cctgcctcca tctcaacggg
     3481 atttcggtat cgttttccaa tcctatgcgc tttttccgaa cctgacggtt ttccagaatg
     3541 tggcctatgg tctggaaaat caaaaaattc ccaaaccgga aatccggaaa caagtcaatg
     3601 atttattgga acttgttggt atgccggaac aaaccgggaa atatcccgca cagctttcag
     3661 gaggacaaca gcagcgtgtc gcacttgccc gtgcactggc tacttcaccg ggattgctgc
     3721 tgctcgacga acctctgagt gcacttgatg cccgtgtccg cgccacatta cgccttgaaa
     3781 ttcgttcttt gcacgaacga ctcggcgtga ccacaattct ggtcacacac gatcaggaag
     3841 aagccctttc gatggcagac cgaatagtgg tgatgaatga aggtcgaatc gaacaggttg
     3901 gaacaccaga ggaaatttac agtgcacctg cctcaccttt tgtcgcagat tttatcggcg
     3961 tgatgaattt tttgcaggga gaagtaacaa actcagggaa ggtgcttatt ggagaaaaca
     4021 cacttgattg tgaaaccgca aacattccgg agggcagtaa agttcgggtg acggtacgac
     4081 ccgaagatat tttatgcagt gcggatgata aaaatgcagc gaattcattt tcggttaaag
     4141 ttgaatccgt cgaatttctc ggttctttct gcagactgat tttatcaggc caggaaggct
     4201 ttgggaaact gatgacagac ctttccacgc agcaagtcca tgaactgaat gttactcctg
     4261 aaaccacact caccgccgta tttcctgcac ggagtctcaa ggttttcccg gaaagcttat
     4321 gagtaccgca gattctattc ccgcgtctac tgttcctgcc aggggagcct ccagcgtacg
     4381 tatcaagctc agtcgggatg actatgcttt gcggaccgga ctcgtggtcg taatcagttt
     4441 gctggcaatt ttagtagttt taccgcttta ttccctgctc tccaaaagtc tggaagacat
     4501 ggacgggaat tttgtcgggt tacagaactt tcaagaatat tttcaaactc cggcgctttt
     4561 aacttctatc acaaacagtt taagcgttgc ttttttttcg gcgataattg tgttggtgct
     4621 ggccttcatt tatgcctacg cgctgacccg aaccaaaatg ccgtttcgcc tgttttttcg
     4681 gggagtagcg ctgataccaa ttcttgcacc ttcgctgctc gcggcaattt cattgattta
     4741 ctggtttgga actcaaggcg tacttaaatc gtggttgttc ggcgcttcga tttacggccc
     4801 catcggtgtc attatggctt cgtgtttctg ggtatttcct catgcgctga tgattttaat
     4861 tacagcactt tctacggcgg atgccagact gtacgaagca gctgaagcct tacgaactcc
     4921 taagtggcgc atattttgga ccgttacgct accgggagcg aagtacggaa ttctcagcac
     4981 aggcttcatt gtttttacac tcgtcatcac agattttggc gtaccgaaag tcatcggtgg
     5041 aaattttaat gtgctggcga ctgacgtgta caaacaagtc gtcggtcagc aaaactttga
     5101 gatgggcgct gtagtttcgc tggttttgct cctgcccgca gtagccgctt ttatcgccga
     5161 ccgctgggta cagagcaaac aagtcgcctt actttccgca cgcgcagtac cttttcaacc
     5221 aaaatctgaa cccttgcgcg actggctcct gtttggattt tgtgcaacaa tagcattcat
     5281 tttcctggca atgctgggga tggctgccta cgcttctttc gtcacattct ggccgtacaa
     5341 tctcacgctt tcactcaata attacgactt cgatttgatg gatggcggtg gctgggaagc
     5401 ctatttcaat tcaatccgga tggctattta ctgtgccgtg tttggttccg ggctgatatt
     5461 cttcggcagc tacctggttg aaaaagcaag aggggtttca tggctgcgta ccattttaca
     5521 tttgatcgcc atgatgccgc ttgccattcc cggattggtt ttggggattg cctacatttt
     5581 cttttttaat catccagaca atccgctcgg tttcctttat ggaaccatgg gaattcttgt
     5641 gatcagcaca attgttcact tttacaccgt cagccatctt acctctctca ctgcacttaa
     5701 gcagcttgat ccggaatttg aaaccgtttc actttccctg aaaacacctg tctggaggac
     5761 ctttgggaaa gtcactcttc cggtttgtct accggctttg ctggatgtgg cgagctatct
     5821 ttttttgaat gcgatgacca ctgttgctgc tgtggttttc ctctattctc ctgaaaccat
     5881 gctggcctca atcgccgtgc tgaacatgga cgatgcagga gacattgcac ccgccgctgc
     5941 catggccttg atgattgttt atacttctgc cgccttccgc ttgctccacg ctctgcttac
     6001 ccgttatctc aaacgccgca ctcaagtctg gcgtaatagt tgatcaaaaa aatgctcgtc
     6061 tcctgttagc tcaactttgt attcgacgca ggtttacggg acaggaaata atttgatcct
     6121 gtaagtttgc gttcacctgt gtgtgttatt tatctggatt agatgactga tttgttggaa
     6181 ctagtaacaa ctgtaggtca tgttactttg aggacttcaa ccttgaagat caagtctgca
     6241 tttggcggga tgcccatcga ggaaacacct tgctgaccgt aggccagttt ggctggaatt
     6301 tttatttgga gcttcgttcc ctctctcact cctttgagag cttcgtccca tcctgagatt
     6361 acactacctt ttccgaggtc aaattcaaaa ggacgacgct tagcccggct gctgtcaaac
     6421 attttgtaat cctcagccaa ccagccttcg tagtggactt tgatgtggct gccgtctttt
     6481 gtcttatctc cgtgaccagg acgttctaca tagacttgaa tcccggatct ggtgtgttta
     6541 aaagcagcat ccagggccag ctggggtcgc ttatttgttt tagcagcttg ctgctgagtt
     6601 accgcggatg taccgacttt catctacagg caaagatggc aggagcttgt tctccaagta
     6661 ttgtcatttg tataggtgcc ttgttgtcac cctgagcaat tcctttgttt acatcaaggt
     6721 ataaattgat cgtgttaccc tggaggtgat atttaccgga ctgaacttcc gcgctgcctt
     6781 caagtaaaat ggtttgctta atctcatcaa agttggcttg atcagctttg gcgacacgac
     6841 ccggttgttc aaaacatact ccctgttcag cacgaatgga ctgtaattcc tgtaactcat
     6901 taagccgtag agtgattttt cctgcatgaa tatagagatt atccggagta cgctccattt
     6961 ccacgtcacc ctcaaacata gccagatgtt tgatattatc caaaacccca cgggaagcta
     7021 acaacagcac tttcttctgt tccgtctttt cttgattttc tgtttcttct tcctgagtct
     7081 tgatttttga gctttgttcc ttgcggtttg tttccaattc gctgagattg gcctcaacct
     7141 taattcgtcc ttctttgctg gtatcctttc cttccagggt tgcatgctga cgcagaatat
     7201 caacaaaaac agtgttactg attacactgc cttgactggc tttcatgatc acatttccct
     7261 gcaaccgcaa tgtctgctgt ggcgtctcga tcgattttgt ttctgcagaa tctgtgtccg
     7321 gagtttgatc ttcaagatca atctgcgtat aaatcgcaaa atctgagcga acctcacggt
     7381 cccactgttt aacgctcact ccaccttcgc cgatcaactt ctcaaagttt tttccttttc
     7441 ccgaaaccag acgaagtttg gctgtagtta attcaatgtt ttctctggga ataacacctt
     7501 taacatttcc gcgaaaaact gctgcattct cattaagatc gctgctgaat tcgtctgcct
     7561 gtatttttat ttgctgggtt gatgcatagc gcatgagaag atcactttcc tttttagtga
     7621 gttgtatcgg cagcgcttca agaaaatcct taaggttaag gtagtgtgtg ttcaggattg
     7681 gtgaaagcat ttcagttagt gcttttgaga agccttctct ttgcaggtga tccagtgtat
     7741 tgcgggtgat ttcgtaacca cttgcaggtg ccgcaaggct tgcttgtcta tagttccatg
     7801 caggttcagt tgtagcaata ctgaaaaata acaagaagac agaaaaaata tttttgattt
     7861 tacgcttaag catttgacgt gtcggctatc ttttttgagg ttgcttgatg atctgaacat
     7921 gactgccggc taagatagga cgcctcaatg aaagaagtaa acaaaggatg cggcttaagt
     7981 ggttttgact gcagttccgg atgatattga cagccgataa accagggatg ttctgcaagt
     8041 tcaatcgttt ctaccaaatt gcgttccggg tgacgccctg aaacagtcag tcctgcctct
     8101 tcaaatctgg gaacaaagta attcatcact tcatagcggt gcctgtggcg ctctgaaata
     8161 gttgttttgt tataaatttt ttctatcctg gttccttttt ccagaattgt ggtttggctg
     8221 cccagacgca ttgtaccacc tgtttcctct tgtccctctt gttcaggcat taaatcaatc
     8281 acgttttccg gatggtccgg acgtgactcc ccggaatttg ccccttcaat tcctacaaca
     8341 tttctggcaa attccacaat tgccatctgc attccaagac aaattccaaa aaatggaata
     8401 ttctttgtcc gtgcaatttt gatagcagca atttttccct cagttccacg ctctccgaat
     8461 ccgggcgcaa ccaaaacacc gtccattttt tccaattgac tcaacacgtt tttctctgtc
     8521 aataattcag aatcaacata gtgtagattt actttaagcc ggtttgcaat gccgccatgc
     8581 agtaaagctt cgttgaggct ttcataggaa t
//
LOCUS       DTVD01000118            8569 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2259_length_8569_cov_547.803500, whole genome
            shotgun sequence.
ACCESSION   DTVD01000118 DTVD01000000
VERSION     DTVD01000118.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_8569_cov_547.803500"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="EYO68_04075"
     CDS             complement(<1..629)
                     /locus_tag="EYO68_04075"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIC07015.1"
                     /translation="MQTKFIFITGGVVSGLGKGIAAASIGALLESRGLTVSLMKLDPY
                     INVDAGTMNPFQHGEVFVTVDGAETDLDLGHYERYTNAVLNRRHNRTTGQIYDAVIRK
                     ERAGEYLGATVQVIPHVIDEIKDTISEAARLSDNNPKTAAASNADICIVEIGGTVGDI
                     ESLPFLEAIRQYRHDHGRENTLFVHLTLIVQTSEMKTKPTQHSVGKLRE"
     gene            complement(723..1142)
                     /locus_tag="EYO68_04080"
     CDS             complement(723..1142)
                     /locus_tag="EYO68_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HIC07016.1"
                     /translation="MKIYQQILQIQTSGKGLQEITRKVQQVISESEITSGLCNIFVRH
                     TSASLVIQENADPDVIQDLEYFFGKLVPENDLGYTHTTEGPDDMPSHIRSALTKTSEQ
                     IPLAGAKMLLGTWQGIFLWEHRSRGHSRNVVVHICGD"
     gene            complement(1157..3385)
                     /locus_tag="EYO68_04085"
     CDS             complement(1157..3385)
                     /locus_tag="EYO68_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIC07017.1"
                     /translation="MPSRLKNLLKNYKELIATDSNIRNRMLAVVVLLFLLIVLVMFSM
                     QQGFSVLEQSGSQLMVFVAINVNIVLLAIVFYLIARNLLKLSYERRRHVLGVNLKTKL
                     ITSFIILSLPAMGFHLFASFFIATNLETWLKGQQESMVHSAQNISEAYHNNLRKTMEL
                     QNLIWENHLKKNEGELSQLKPPNNIDADVTLYSENQELLKQWMQSEISQAYWSTPSLN
                     NWYQLKTKDQTWFTEELEDRFIYRHIRPVSIGNRKLYLEVFFPATPYASGAINDIIAQ
                     DQNTRFLTESEDLVRGYYLIIFLLMTLFIIFVATWLAFYLARGFVQPIEDLALATQRV
                     SEGELGYQVELRGTLDKDFALLMQSFNSMSRDLLEHQVALDKTTNHLQESHRTLESQI
                     RFVELVLENITTGVMSLDMNGHIEILNRSAKQMLQLKTGSEGSKHYREVLEKESLIMF
                     EEMVKQIENTKERSISRNLVVHKKEGPIQVSATLLLLQNSEGHSAGMVCVYNNITEIQ
                     RLQRARAWREVARRIAHEIKNPLTPIQLSAERIHRKYAGEVSDDVALQQSTQTIINEV
                     QHLKRMVSEFSNFAKIPESNPQMSNLNQIINEALQLYHENMPARISLTTELSETLPQL
                     PLDGEQIRRVVINLVDNAISSIEKKGALSRIFRQGQIIVRTRHVPDLNIISMDVEDNG
                     TGIAPEISDDLFEPYTTTKEHGTGLGLTIVSQTISDHNGFTRFRNLDTGGVCFTIELP
                     VT"
     gene            complement(3472..3909)
                     /gene="ccmE"
                     /locus_tag="EYO68_04090"
     CDS             complement(3472..3909)
                     /gene="ccmE"
                     /locus_tag="EYO68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIC07018.1"
                     /translation="MKTRNKFVIGGAVIFAVIAALSVQGLQEMTVFFYTPAEVLASPD
                     EFENKTIRIGALVQKDSVEWDAQAIELSFKITEDSKNFIPVFYAGVKPDLFREGQGVV
                     VEGKMKGQRFVAGQLLVKHSEEYTVETDHKKSKEDYYRSIQAQ"
     gene            complement(3893..4078)
                     /locus_tag="EYO68_04095"
     CDS             complement(3893..4078)
                     /locus_tag="EYO68_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07019.1"
                     /translation="MLIGLEYVVAAYGIWVGTFAIYIFLTKRSMKNADQTVTLLEQRV
                     SESRKKSIPTENNENSK"
     gene            complement(4079..4777)
                     /locus_tag="EYO68_04100"
     CDS             complement(4079..4777)
                     /locus_tag="EYO68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HIC07020.1"
                     /translation="MASMKKIILYLEWGSILGLLACGLWVFLKLPLESSQGFPQKIMY
                     LHVPTFLSTYLAFFVVFTFSIAYLWKRELVFDQIAKASAEVGLVFCALLLATGAIWGR
                     TTWGTYWVWDARLTTTLLLFLIFMGYFLLRMSVNDRDKEARLASVLGIIGCLDIPIIH
                     KSVQWWRTLHQPSTLFKVESGAAKPSMPPELLYPLIASAIVMLVFYLYLLLLRLQTEK
                     RRETLNQLLAEIQN"
     gene            complement(4770..6203)
                     /gene="gatB"
                     /locus_tag="EYO68_04105"
     CDS             complement(4770..6203)
                     /gene="gatB"
                     /locus_tag="EYO68_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIC07021.1"
                     /translation="MQFEPVIGLEIHVQLATASKIFCCCSTEFGNPPNSNTCPVCLGL
                     PGALPVLNSQVPEFAAQLGLALNCDIRLDSQFARKNYFYPDLPKAYQISQFDRPICEN
                     GWLEIETESRGKRRIGITRIHMEEDAGKLVHADGSTESLVDLNRAGVPLVEIVSEPDV
                     RSAEEAKAYMEKIHAIATTIGVTDGDMEKGHLRCDANVSLRPVGQEEFGTRTETKNLN
                     SFRFVQQAVVYEIGRQREEILDGKQIVQETRLWDTGRKVTFSMRSKEEADEYRYFPDP
                     DLPLVHLAPELIETFRKNLPELPDAKRQRFMEEHGLSEYDAEVLSADRRMSEYFEKVL
                     TYGAAPKLACNWIIGDLTRVMNESGKSLKEIDLSPEHLAALTKIINSGEISSKIAKTV
                     FEEMLASARTPEEIIEEKGLKQVSDSGELGRIVDELLAANPEQVEQYRAGKTKVIGFF
                     VGQMMKQTQGKGNPAVINSLLKEKLDG"
     gene            complement(6380..7039)
                     /locus_tag="EYO68_04110"
     CDS             complement(6380..7039)
                     /locus_tag="EYO68_04110"
                     /inference="COORDINATES: protein motif:HMM:PF05014.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07022.1"
                     /translation="MSQKYSIYFAGDLFNHKDLIGNLLLSEAIEKNSAGRFVCVVPQH
                     LEQSTNRSIDIRNNDLSEVVKADLILLNFDGTELDSGTVIEFLFAKALDIPAVILRSD
                     FRSAGDQERGDPWNLMCSGYPRTRTIKLNSMAWYQEAWGKGGGTSAILERFYDKLSKL
                     INSEFDLVLKEASVVDNKQMLQNVYHWALRFPGNGFSDLFSEDELKTLLEAKQKKGSV
                     L"
     gene            complement(7083..7619)
                     /locus_tag="EYO68_04115"
     CDS             complement(7083..7619)
                     /locus_tag="EYO68_04115"
                     /inference="COORDINATES: protein motif:HMM:PF06941.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07023.1"
                     /translation="MKICSDVDGVILDYIRGFIEFTQREKIPYTHDPELYGVIRNFPD
                     NFEVRDRFHAGDSLRNLKYIDGSLEILNLLASRHELHLVTALEPEQTQKRAENLKSVN
                     YTTLQCVGDLRKEQIIVGEIQPDVMIEDRPELIESFCSAGLYVIYPDWHPYTKGMDRY
                     ATPFSHWQEVPDLLKKAS"
     gene            complement(7684..8088)
                     /gene="msrB"
                     /locus_tag="EYO68_04120"
     CDS             complement(7684..8088)
                     /gene="msrB"
                     /locus_tag="EYO68_04120"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIC07024.1"
                     /translation="MTDFKSKTEGEWKEQLTPEQFEICRKKGTERPFSGEYVETKTRG
                     TYHCLCCGNALFLSETKFDSGSGWPSFTDVLGDDNVSTQEDLSLSMQRTEVVCRQCDA
                     HLGHVFEDGPAPTGLRYCINSVSIVLEEDKES"
BASE COUNT         2417 a         1902 c         1682 g         2568 t
ORIGIN      
        1 atttctctga gttttcccac actgtgctga gtaggcttgg ttttcatttc agatgtctgc
       61 acaattaagg taaggtgaac aaacaaagtg ttttctcttc cgtgatcatg ccggtattga
      121 cgaattgcct cgaggaatgg cagggactca atatctccaa ctgttccacc gatttcaaca
      181 atacaaatat ctgcgtttga cgcagccgcg gttttagggt tattatcgga caacctggcg
      241 gcttcagaaa tcgtgtcttt tatttcatca atcacatgtg gaatcacctg aacagttgca
      301 cccaaatatt ccccagctct ttctttgcga ataactgcat cataaatttg accggttgta
      361 cggttgtgcc tgcggttcaa cacggcattt gtgtagcgtt cgtaatgtcc aagatccaag
      421 tctgtttctg caccatccac cgtgacaaaa acctctccat gctgaaaagg attcatggtg
      481 ccggcatcga cattgatgta gggatccaat ttcatcaagc tgaccgtcaa gccccttgat
      541 tccagcagtg ccccgataga agctgcggca atacctttcc ccagtccgga aactactcct
      601 ccggtaataa aaataaattt agtttgcata aattaagtgt tttgtttatt ggaattaatt
      661 ttcagttaaa aaagtttgaa ctttaaaaca gcatgaaagt tttctcaagc caaatgaaca
      721 aatcaatctc cgcaaatatg gacgacaaca tttctggaat gtccacgtga acggtgttcc
      781 cagaggaaaa tcccctgcca agttcctaaa agcatcttag caccggcaag cggtatttgc
      841 tcagaagtct ttgtcaatgc actccggata tgcgaaggca tatcatccgg accctccgta
      901 gtgtgagtat aacctaaatc gttttccgga accagctttc cgaaaaaata ttccaaatct
      961 tgtatgacat ccggatccgc gttttcttga attacaagac tggctgaagt gtgacggacg
     1021 aaaatattac agagtccgga agttatttca gactcactaa tcacttgctg gaccttgcga
     1081 gtgatttcct gaagcccttt cccactggtc tgaatttgga gaatttgctg ataaatcttc
     1141 attgcagatc tgcagttcag gtgacaggaa gttcaatcgt aaaacagaca cctccggtat
     1201 caagattccg gaagcgggta aagccgttgt gatcggaaat agtttgactg acaatagtca
     1261 aaccaagacc ggttccatgt tcttttgtcg tcgtgtatgg ttcaaaaaga tcgtctgata
     1321 tttctggagc aataccggtt ccgttatcct ctacatccat gctgatgatg tttaaatccg
     1381 gaacatgacg ggtacggaca ataatttggc cctgacggaa aattcgtgaa agtgcccctt
     1441 ttttttcaat tgaactgatt gcattgtcca ctaaatttat gactacacgc ctaatttgtt
     1501 ccccgtccag cggtagttgc ggcagtgttt cactcaactc tgtggtcagt gaaattctgg
     1561 caggcatgtt ttcatgataa agctggagtg cttcattgat aatctggttc agattactca
     1621 tttggggatt tgattccgga attttagcaa aatttgaaaa ctcactaacc atccgcttga
     1681 ggtgttgaac ttcattgata atagtctgag tcgattgctg aagggctaca tcatccgaaa
     1741 cttctcctgc atatttcctg tggatccgct ctgcggaaag ctgaattgga gtgagtggat
     1801 tttttatttc atgtgcaatc cgtcgcgcta cttcccgcca tgcccgggca cgctgcaatc
     1861 gctggatttc agtgatattg ttgtaaacgc aaaccatgcc ggctgaatga ccctcactgt
     1921 tttgaagaag aagcagagtt gctgaaactt gaatgggccc ctctttttta tggactacca
     1981 ggttacggga aatggaacgt tcttttgtgt tctcaatttg ttttaccatt tcctcaaaca
     2041 ttatcagaga ttccttttcc agaacttccc ggtagtgttt gcttccctca ctgccggttt
     2101 tcagttgaag catttgtttt gcggagcgat tcaatatttc aatgtgaccg ttcatatcca
     2161 atgacattac gccggttgtg atgttttcca aaacaagctc tacaaagcga atttgacttt
     2221 ccagtgtgcg gtgactttcc tggagatggt tggttgtttt atcgagtgca acctgatgtt
     2281 caagcaaatc acggctcatt gaattaaagg actgcatcag cagggcaaaa tctttatcta
     2341 aagtaccacg gagttctact tgataaccca attcaccttc agaaacgcgc tgtgtggcaa
     2401 gtgccagatc ttcgattggc tgaacgaatc cgcgcgccag atagaaggcc agccaggtgg
     2461 caacaaaaat aataaacagg gtcataagga ggaaaatgat taaatagtaa cctcgtacca
     2521 agtcctctga ttcagtcaaa aaccgtgtgt tctgatcttg agctataata tcgttgattg
     2581 caccggaagc atacggagtg gctggaaaaa aaacttctaa atacaacttt ctgttcccaa
     2641 tggaaaccgg tctgatgtga cggtaaatga atcggtcttc caactcttca gtgaaccagg
     2701 tttggtcttt agtttttaac tgataccagt tgtttaacga aggtgttgac caataagctt
     2761 gtgaaatttc gctctgcatc cattgcttca gcaactcctg attttcagag taaagtgtca
     2821 catccgcatc aatattgttt ggcggcttca gctgacttaa ttctccctcg ttttttttca
     2881 aatggttctc ccagatcaaa ttttggagtt ccatggtttt gcggagattg ttatgatagg
     2941 cttcagaaat gttttgtgca ctgtggacca tggattcctg ctgacccttg agccaggttt
     3001 ccaagtttgt ggcaatgaag aaacttgcaa acaagtgaaa gcccattgcc ggtaaagaga
     3061 gaatgataaa agaagtaatg agtttcgttt taagattgac acctaaaacg tgacgacgac
     3121 gttcataaga aagcttgagg aggttacggg caatcagata gaaaactatt gcgagcagga
     3181 caatattaac gttgattgcc acaaaaacca tcagttgtga acctgattgc tccaaaactg
     3241 aaaaaccctg ctgcatactg aacattacca gtacaatcag caaaaacaac aaaacgacta
     3301 ctgccagcat cctgttacgg atatttgagt ctgtagctat cagctctttg tagtttttca
     3361 gtaaattttt tagacgagat ggcatttttt aggtgggtcc aggtagagag ttttcaggaa
     3421 tcggagttca gaagtacttg aattctgtct ccaaatccaa aattaccagc tttattgcgc
     3481 ctgaatcgat ctgtagtaat cttctttgga ttttttgtga tccgtttcaa ctgtgtattc
     3541 ctcactgtgt ttgaccagca attgtcctgc aacaaagcgc tgccctttca ttttaccctc
     3601 cacaacaaca ccctgtcctt cacggaacaa atctggttta actccagcat aaaaaacggg
     3661 gataaaattt ttgctgtctt cagtaatctt aaatgaaagc tctattgcct gtgcatccca
     3721 ttcaacactg tctttttgta caagcgcacc gatacggatt gttttatttt caaactcatc
     3781 cggactggcc aggacttctg ccggagtata aaagaatacc gtcatttctt gcaacccctg
     3841 cactgataag gctgcaatca ctgcaaaaat aactgcccct ccaataacaa atttatttcg
     3901 agttttcatt attttccgta ggaatagatt tttttcgtga ttctgaaact ctttgttcca
     3961 gtaaagttac agtttggtcg gcatttttca tgctccgctt tgtcaaaaaa atataaatgg
     4021 caaatgttcc aacccaaatt ccgtatgctg caactacata ttctaaacca attagcattt
     4081 aattctgtat ttctgcaaga agttgattga gagtttcccg gcgtttttct gtttgcaagc
     4141 gcaggagcag caaatataaa tagaaaacca gcattactat tgctgaagca atgagtggat
     4201 aaagtaactc cggaggcatt gacggttttg ctgccccgga ttcaacttta aacagtgttg
     4261 aaggctgatg taaagtgcgc caccattgaa ctgatttgtg aataatgggg atatccaggc
     4321 aaccaatgat tcccaaaact gaagcaagtc gtgcttcttt gtctctgtca ttaacagaca
     4381 tccggagcag gaaatagccc ataaaaatta aaaacaacaa cagagttgtc gttaggcggg
     4441 catcccaaac ccagtacgtt ccccaggtcg ttcgccccca gatggcaccg gtcgccagta
     4501 atagagcaca aaagaccaga ccaacttccg ccgatgcctt tgcaatttgg tcaaacacca
     4561 gctcacgctt ccaaagatag gcaatgctga aagtaaacac cacaaaaaaa gccaaatatg
     4621 tggaaaggaa tgttggtaca tgaaggtaca tgattttttg cggaaatccc tggctggatt
     4681 caagcggaag tttcagaaaa acccaaagac cgcaagctaa cagacccaaa attgatcccc
     4741 attccaaata aagaataatt ttcttcatac tagccatcca gcttttcctt taagagactg
     4801 tttattacag ccggattgcc tttaccttgt gtttgtttca tcatctgacc cacaaaaaaa
     4861 ccaatcactt ttgttttacc tgcacgatat tgttctacct gttccggatt tgccgcaagc
     4921 agttcgtcta caatccggcc cagttctcct gaatcagaaa cttgcttcaa gcctttttct
     4981 tcaataattt cctccggagt ccttgctgaa gcaagcattt cctcaaaaac agtttttgca
     5041 atcttgctgc tgatttcacc gctattgatg atttttgtta aagctgctaa atgttccgga
     5101 gacaaatcaa tttccttgag agattttccg gattcattca tcacacgtgt caaatcaccg
     5161 atgatccagt tacaggcaag ttttggcgct gctccatatg tcaaaacttt ttcaaaatat
     5221 tcagacattc tcctgtctgc agaaagcact tccgcatcgt attcgcttag tccgtgttct
     5281 tccataaaac gctgtctttt tgcgtcagga agttctggaa gatttttgcg gaaagtttca
     5341 atcagttccg gagcaaggtg taccagcggc aaatccggat ctgggaaata gcggtattca
     5401 tcagcttctt ctttggaacg catactgaag gtgactttac ggccggtatc ccagagtcga
     5461 gtttcctgca caatttgttt cccatctaaa atttcttcac gctgacgtcc aatttcgtaa
     5521 accacagcct gttgaacgaa gcggaatgaa tttagatttt tagtttctgt acgtgtccca
     5581 aattcttcct gcccgactgg acgcagtgac acatttgcgt cacaacgtaa atgtcctttt
     5641 tccatgtctc catcagtaac accaattgtg gtggcaattg catggatttt ttccatatag
     5701 gctttggctt cttctgcaga acgtacgtcc ggttcactca caatttccac caaaggcact
     5761 ccagcacggt tcaaatcaac caaactttca gtgctgccgt ctgcatgaac cagtttccct
     5821 gcatcctctt ccatgtgaat acgggtaata ccaatacgtc ttttgcctct ggattcagtt
     5881 tcaatttcca accatccgtt ttcgcaaatc ggtcggtcaa actgggaaat ctgataggct
     5941 ttaggcagat ccgggtagaa gtaatttttt ctggcaaact gagaatccag ccgaatatca
     6001 caattcaacg caagtcccag ctgtgctgca aattccggaa cctgactgtt cagaacaggc
     6061 agggcacctg gcagacccag acaaaccgga caagtgtttg aatttggcgg attaccgaat
     6121 tctgtggaac aacaacaaaa tattttggat gcagtggcca gctgaacgtg aatttctaaa
     6181 ccaattacag gttcaaattg cattaaaatc tttttgatta agttactaga cagtcacaag
     6241 ttttattgtg tcatcccgaa tgtatatgag gaatcttata agatttcagc attttctcag
     6301 atctccccct tcggtcgaga tgacatatcg cagtcaaaaa tataagtatc tttcgtgaat
     6361 tcataattca cgatttaagt tataaaacag agcccttttt ttgtttggct tccagtaagg
     6421 ttttgagttc atcctcacta aacaaatcag agaatccatt ccctgggaaa cgtaatgccc
     6481 agtgataaac attctgcagc atttgtttat tgtcaacaac agaggcttct ttgagaacca
     6541 ggtcaaattc tgaattgatt aattttgaca atttatcata gaaacgttcc agaatagctg
     6601 aagttccgcc gccttttccc catgcctcct gataccaggc cattgaattc agttttattg
     6661 ttcttgtacg ggggtatccg gaacacatca aattccaggg atcaccacgc tcctgatcgc
     6721 ctgctgagcg gaaatccgag cgcaaaataa cggctggaat gtcgagtgcc ttggcaaata
     6781 aaaactcaat taccgttccg gaatcaagct cagtgccgtc aaaatttagc aggatcaaat
     6841 ctgcttttac tacctcactc aaatcattat tgcggatgtc aatggagcgg ttcgtacttt
     6901 gttccagatg ctgcggaacg acgcatacaa aacgccctgc ggaattcttt tcgatagcct
     6961 cagacagcag caggttgccg attaagtcct tatgattaaa caaatcgcct gcaaaataaa
     7021 tcgaatattt ttgtgacatt aaagctattt tagtggaaga atcagataaa aaattggttt
     7081 gtctacgaag cttttttcag caaatccgga acttcctgcc aatgagagaa tggggtggca
     7141 taacggtcca tcccctttgt gtaggggtgc cagtcagggt aaatcacata aagtccggcg
     7201 ctacaaaaag attctatcaa ttccggacgg tcttcaatca ttacatcagg ctgaatttcc
     7261 ccgacaataa tttgctcttt gcgcaaatca ccaacacact gcagagttgt gtaattcaca
     7321 gatttcaaat tttccgcacg tttttgagtt tgttccggtt ccaaagcagt cacaagatgc
     7381 aattcgtggc ggcttgccaa caaattcaat atttccagcg agccgtcaat atactttaaa
     7441 tttcgcaatg aatctccagc gtgaaatcga tcacgcactt caaagttgtc agggaaattc
     7501 cggatgacac cataaagttc cggatcatgg gtgtagggaa ttttttctct ttgcgtgaat
     7561 tcaataaaac ctcgaatgta atccaggatt actccatcta catcggaaca aattttcatc
     7621 tgtgtctgga cctttcaaaa ataattcagt caatctgtag cttgacagaa aaagactccc
     7681 caatcaagat tccttgtctt cttccagcac aatcgaaaca gaattaatac aataacgcaa
     7741 acctgttggt gcaggaccat cttcaaaaac atgtcctaaa tgtgcgtcac actgcctgca
     7801 aactacttct gttctctgca tggacagact caggtcttcc tgtgtactca cattgtcatc
     7861 accaagtaca tccgtaaaac tcggccagcc gcttccggaa tcgaattttg tttccgacag
     7921 aaacaaggca ttgccgcaac acaaacaatg gtatgtgccc ctggttttag tctccacata
     7981 ctctccgctg aaaggtcgtt ctgtaccttt ttttctacag atttcgaatt gttcaggagt
     8041 cagttgctct ttccactccc cctcagtttt ggatttaaag tcagtcaagt ttatttatca
     8101 ttaattgttc aacaatactt ttcttgtact taatattact caaaactatt tttttaatac
     8161 tcagagctgt taattgattt tcaaaatata aagtccctag ggtagcatga tacttgtaat
     8221 taaatcaata ataatgcaga atgatccctg ccgcaggatt tagtgttttt tagttgcaaa
     8281 gatgagtcag tggttacaaa tcgttacact gcagaaatga tttaagcttt aaatttagct
     8341 aaactgggtc atgctttgag ctgtattgtt taattccagt aattctcttt tactcttacc
     8401 tacaaaagtt gtctttgaaa aatttaataa aaacacaaat tcttttaacc ttgttttgct
     8461 tcgtctggat gggttttgct caggctcaaa accaaacttc ttatacagag gaaactgcaa
     8521 agttacttga acaagtaaac ttgaaaacgg ttgacggccg tgaaatggc
//
LOCUS       DTVD01000119           27736 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_225_length_27736_cov_538.793613, whole genome
            shotgun sequence.
ACCESSION   DTVD01000119 DTVD01000000
VERSION     DTVD01000119.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 27736)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27736)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27736
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_27736_cov_538.793613"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="cbiE"
                     /locus_tag="EYO68_04125"
     CDS             complement(<1..842)
                     /gene="cbiE"
                     /locus_tag="EYO68_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HIC07025.1"
                     /translation="MPDISKKWLCVIGIGEDGWDNLSADARDLLYESEIVLGGERHLK
                     MLPKDWEGERIIWSSPIREAVSKIVSWRPEDSASAQKVAIMASGDPLCYGIAAKLLRH
                     LPIEEIWIKPALTTFSLMCSRIGWSLPDVETLTIHGRPLEMLHPFVQPGAKLLVLSKG
                     EESPEQAAKLLCARGFGKSIITVLEHLGGRKERQFSGQADSWNHPEGAALNAMAIECI
                     PENNANVLSRNPGLPDEAFLHDGQLTKREIRAVALSKLMPVVDHLLWDVGAGCGSVAI
                     EWMRT"
     gene            complement(844..1509)
                     /locus_tag="EYO68_04130"
     CDS             complement(844..1509)
                     /locus_tag="EYO68_04130"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIC07026.1"
                     /translation="MTGDYLRDPKEIYRQSFATILKEADLSKLPEDIADLTVRLIHAC
                     GMPDIVEDLVFTPDAAQAGYAALLKGAPVLCDSRMVAEGIIRSRLSANNEVKCWNNHP
                     ECAPLADKMETTLSAAAVEFWRPQLAGAVVAVGNAPTTLFRLLEILAEGVEPPAVIFG
                     FPVGFIGAAESKELLVKQCVGAVPFLTLSGRRGGSAMAAAAVNALAKKAEISVANNIN
                     IQS"
     gene            complement(1506..2546)
                     /locus_tag="EYO68_04135"
     CDS             complement(1506..2546)
                     /locus_tag="EYO68_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIC07027.1"
                     /translation="MKEQYGVMVCGHGSRDTGAVEEFQAVAQGLKERLPQYETDWGFL
                     EFATPVIRTGLDALRAKGIRKVLAVPGMLFAAGHAKNDIPSVLNAYQAQNPELEISYA
                     RELGIDLKLIRAAGERVEDALQKAGDGVSREDTLLVVVGRGASDPDANSNVAKVTRML
                     WEGLGLGWAETAYSGVTFPLVEPGLEHATRLGYKRIVVFPYFLFTGILVQRIYHYTDI
                     VAAKHPEIEFIKASYLNDHPLVLDAFAERVEEIRNGENNMNCQLCKYREQFLGFENEV
                     GLRQESHHHHVEGVGTGTDESSDAVEDAHSHVHEHSHDHNHNHGHHPYPHADHPLGPV
                     TLEEKSGKSGAE"
     gene            complement(2825..3301)
                     /locus_tag="EYO68_04140"
     CDS             complement(2825..3301)
                     /locus_tag="EYO68_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002352084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIC07028.1"
                     /translation="MRLHKNPPLNIVLYEPQIPPNTGNIARLCAATKCHLILLGKLGF
                     DLSDKQLKRAGLDYWQWVSWDHFPDAEDFFSTLVPENFHFLSVHNERPYTKIRPQEGD
                     YLFFGKETSGLPDEWMERFPERCFTLPMWEKGVRSLNLGSSVAVVVYDGLRHLGKF"
     gene            complement(3356..4420)
                     /locus_tag="EYO68_04145"
     CDS             complement(3356..4420)
                     /locus_tag="EYO68_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIC07029.1"
                     /translation="MSKKLTIGLICGGTSGEHEVSLISAYNIQAALDRDKYDVLLIGI
                     DKSGAWFLGKTPEFLDNVDDVRQVKFRKNLPILLPQTGNSAGENLASTEQSGNELANV
                     DVFFPITHGKLGEDGALQGFLELLGKPYVGADVYGSAICMDKDVSKRLLLQRGITVAR
                     YKLIRNPDELSFVDAVEELGEPLFVKPCREGSSLGVSKVRTVKEYTQALEMAFAADRK
                     VMVEEAIYGREIECSVLGNDSPEAAAVLGEIVPLREFYSYEAKYVDEDGAELIIPVEI
                     DPDVSMQIRKVAVEAFLLTECRSMARVDFFLREDKTFILNEINTLPGFTNISMYPKLW
                     DASGLAYPELLDRLIELALE"
     gene            complement(4579..6396)
                     /gene="aspS"
                     /locus_tag="EYO68_04150"
     CDS             complement(4579..6396)
                     /gene="aspS"
                     /locus_tag="EYO68_04150"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIC07030.1"
                     /translation="MNKIMEWTRTHYCGDLRIENVDQEVVLMGWCQTRRDHGGVIFVD
                     LRDYTGISQIVFKMEISETSHVLADSIRGEFVLAVKGKVAHRIEGNVNPKLPTGEIEI
                     MVETLEILNKSETPPYVLENRENVDEKLRLTYRFLDLRDSTLQNNLMLRSQSLQIVRN
                     YFTSQRFFEIETPILTKSTPEGARDYLVPSRVNPGLFYALPQSPQLFKQLLMISGYDR
                     YMQLARCFRDEDLRGNRQPEFTQIDLELSFTEPEEIYKLTEGLMVKLFEETISVKVET
                     PFQRMTYQESMENYGSDAPDIRFDLKLKDISDIASECELRVFKQVVDKGGIVKAICVP
                     GGADFSRKDLDDLTEFAQIYGAKGMAWIKRNQDGWQSPIGKFFTSEQKEALEERVGLN
                     VGDLVLFCADNTKVVHDALGNLRKEIALRRGLINNSEYRFVWVTDFPLFEYSETEKSF
                     TSSHHPFTMPDIDDLEKYGEQDPEKICSRAYDVVLNGVEIGGGSIRIHREDIQNKVFR
                     LLKLTDEEIESKFGFLMKALSYGAPPHGGIALGFDRMMMFLLKTDSIRDVIAFPKTQK
                     AGCLMTDAPSAVETEQLDELHIRVKASAQQTEKLELAEK"
     assembly_gap    6423..6522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6546..6911)
                     /locus_tag="EYO68_04155"
     CDS             complement(6546..6911)
                     /locus_tag="EYO68_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017040012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC07031.1"
                     /translation="MNYQSINFSEKLNKFSDLWQPRVIAEMNDYQFKLVKIKGEFVWH
                     EHDDTDEVFIVLEGEMEIEFRDGSVEMKAGEMFVIPKNIEHKPVANNECKILLVEPRG
                     VVNTGDSGGELTAENDVWI"
     gene            complement(7279..7578)
                     /gene="fliE"
                     /locus_tag="EYO68_04160"
     CDS             complement(7279..7578)
                     /gene="fliE"
                     /locus_tag="EYO68_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HIC07032.1"
                     /translation="MNINLPSVGPALQGLKTEAESQKQSVESGISRTFEKLLQDVNQQ
                     QLNAEAKQVELLVTDNKDIHGTMLALEKADLSLRLMLQIRNKLVSAYEEVMRMQV"
     gene            complement(7647..8096)
                     /gene="flgC"
                     /locus_tag="EYO68_04165"
     CDS             complement(7647..8096)
                     /gene="flgC"
                     /locus_tag="EYO68_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HIC07033.1"
                     /translation="MSFQSSLNVSVSGMAAQRQRVNTISENIANASTTRTPQGGPYRR
                     RIVTLAAVSNDRTFEEELRSQQRSLDTATEVKVVGISQDNRAPILRYEPGHPDANEEG
                     YVAMPNVNIMEEMVSLMEASRSYEANTAAFNATKNMAQSALDLGRNV"
     gene            complement(8096..8626)
                     /gene="flgB"
                     /locus_tag="EYO68_04170"
     CDS             complement(8096..8626)
                     /gene="flgB"
                     /locus_tag="EYO68_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HIC07034.1"
                     /translation="MQADHSGYNNQIHSKSGPEIASSSQILLVEYYPKISGFMSGMFE
                     NNLFTLIQKTLDFRTQRQDLLTSNIANKDTPGYKAEDMVFEKSLEKALHAEEPGLLKT
                     TNSRHFDGRNTPPLKLVQAQRIHSASPFPDFDGNTVDLDREMAKMAENQLMYNASIRM
                     LTHQFRMLKTAITEGR"
     gene            8679..10172
                     /gene="argH"
                     /locus_tag="EYO68_04175"
     CDS             8679..10172
                     /gene="argH"
                     /locus_tag="EYO68_04175"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIC07035.1"
                     /translation="MDKKRAQVWGSHLKKPPNEQNILFCAGRDVQPLPMADELLLPYD
                     IWTNRAHCIMLHKQGLIPSASLSSILAGLNKLEKLVAAGNFSLDPAKEDVHINVEAFV
                     TENQGAEAGGRMHIGRSRNDQSACDMRLYLRAVGLSLFDSVKNLAASLLVQAEKNTES
                     VMPGFTHYQPAMVTTWGHWMCAYVQGLCRDLERLAFAISLVNRNPLGAAAAFGTSWPI
                     DRQLTTELLAFEKIDTNTLDCIVSRWENEAQLAHAGSMLMNHLSIMSQDLMFLSHPYS
                     GLLQIDDDYVTGSSIMPQKKNPDFAEVIKSKAALAHGFLSSLLGIQKGGLSGYNRDTQ
                     ATKYLLMDLVRECEAAPIILQGVFESLTINTEKMRELCETDFMNSVDIADHLARTLNL
                     SFRECYHILARAVKLSLPETKITTAALKKALAESDHPTEIAEDLAELHDPQKILKLRQ
                     HQGSPSPEQTSLQIEELSAELNSLSAPLVDLQNNIIAAQENCQNYGV"
     gene            10162..11202
                     /locus_tag="EYO68_04180"
     CDS             10162..11202
                     /locus_tag="EYO68_04180"
                     /inference="COORDINATES: protein motif:HMM:PRK00094.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIC07036.1"
                     /translation="MGSDGLKIGVIGAGAWGTSLARLLASSGNSVQLWCHEPETAKNI
                     LQKHVNTSFLPDILLPESLGASTDLLKIVQNSRILVAATPSHLTREIAKKIRPEITAE
                     HILLILSKGIEQHSLALMSEIFAEELDNLPKLAVLSGPTFAREVALDLPSAAVLACAD
                     ETAGSLLQKCFHSDRFRIYRSTDLVGVQLGGSIKNVIAIASGIADGMQLGLNARAALI
                     CRGVAEMSRLGTAMGGSAETFMGMSGIGDLVLTATGNLSRNHNLGEKLGQGFSLEDCL
                     PSGGAVAEGVRNAVSIQELAERHKIEMPICNAVYQVLHQGVSCTQALQKLLERDRPDE
                     EMYVPDLATMPD"
     gene            11265..12572
                     /locus_tag="EYO68_04185"
     CDS             11265..12572
                     /locus_tag="EYO68_04185"
                     /inference="COORDINATES: protein motif:HMM:PF04932.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07037.1"
                     /translation="MNSFFSTKNWNKFFLYLLFICSTFSIAGTDASITILYLMTLISW
                     IWYRQMSQLRNPLLWSILFFMAAAVLSGLLNAYETEHLMALRTNWRLLLPLLLAVILA
                     DIDEEQLLSVFFGFVMLISIYGIIQYFTGADWLRPEGQQLTTPFSSGTSTESPVFHGK
                     GNFSHHLTYGGFLLLCFPLVCSFIFCKGWSPFARLVTAIIAVVVLLGIGASLGRSIWL
                     GTAVAITILLFRLSPKLMLAFSILVIAGGTYFYSQFSEPSFQNRTPTNRIEALQQRFI
                     SGFMPQSNQDRILMWEAGISAIQDHFWLGIGYGNDSEIMPVYREKISERTGHRFNNSA
                     GTGIHNIYLQTWINYGLFGFLGYLSILIIFFWQSILTLYRTTPYSFENSILWAGIAGV
                     SGFMVAGFFENNFRDGEVQAMLMILIGLNLHQIQKMKERIFSH"
     assembly_gap    12633..12732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12877..14037
                     /locus_tag="EYO68_04190"
     CDS             12877..14037
                     /locus_tag="EYO68_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07038.1"
                     /translation="MGLIIISFFAFVSLGLPDGLMGVAWPGIRHQFQLPVDALGIILV
                     CGTAGYMLSSFLSGLLIRHLGIGGLLSLSCAAMAGTLLVYAVTPLWWVFVIFSAVGGL
                     GAGAIDAGINTYIAKYHSTRMMQWLHASFGVGITSGPFIMTMGINATSHWQSGYFVVS
                     VAMVILSVIFLFTKDMWEGISASSREEDHDENNASLSETIKSIQVLLSVALFFLYTGV
                     ELGLGLWIYSLLTESRGVAPEVAGIVTGGYWAMFTVGRIFAGWYAKKIDIRKLIYLSI
                     SLTLFGVALLLADSSQIITILGVTLAGFAIAPIFPGLVSSTTSRVGFRHQANTIGMQI
                     AAAGLGVAVVPSIAGVLARIFGLEVIPLYLLSTLALLLLVFIISNSHSGENT"
     gene            14080..14604
                     /locus_tag="EYO68_04195"
     CDS             14080..14604
                     /locus_tag="EYO68_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIC07039.1"
                     /translation="MKFINIQYCKTKIGELILGSFEGKLCLLDFRYRKMRKSVDERIK
                     KGLNAKFVENNTEIIERIRAELDEYFHGNRKEFDVPLQMVGTDFQKSVWEALLRVPFG
                     TTSTYLQLAKNIKNEKAVRAVAAANGANSMAIIIPCHRIIGSNGELVGYAGGLPIKKR
                     LLKLEQITTQSGTK"
     gene            14669..15673
                     /locus_tag="EYO68_04200"
     CDS             14669..15673
                     /locus_tag="EYO68_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIC07040.1"
                     /translation="MNNIPQSMNYIQIEKHGDPEVLKLHSMPVPEPGPGEVLIKVAAA
                     GVNRPDVMQRKGLYPPPPGATDVPGLEVSGTVVSVGQNVSEPPINSEVCALVTCGGYA
                     EYCLAAASICLPVPEKISLVNAAGIPETFFTVWTNVFKRGQLKAGESLLVHGGSSGIG
                     TTAIQLGKAFGATVYTTAGTSDKCEFCNNLGADAAINYREQDFSEEIKRLTEGKGVNV
                     ILDMVGGPYFPKNIRLLADEGRLVQIALMQGSKAEVDFRSLLLKRVTLTGSTLRPRSV
                     EEKTKIAQALQKNVWPLLESGAIRPIIHQTFPLKQASEAHRLMESSAHIGKILLKPAD
                     "
     gene            15837..16340
                     /locus_tag="EYO68_04205"
     CDS             15837..16340
                     /locus_tag="EYO68_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIC07041.1"
                     /translation="MDENAKKTALRMIPYGLYVMTAEDKDGRISAATVNWVTQASFKP
                     PLVAVGVKADSQIHDIIKTAGNFALNVLGKGQQGAAYAFFKPAERDGQKISGEPFHAG
                     STGAPVLENTPAFVECRLVTTVEEGDHSIFVGKVVDAGVTQEPEGRADEATLLLKDLG
                     EKTFYGG"
     gene            16354..17274
                     /locus_tag="EYO68_04210"
     CDS             16354..17274
                     /locus_tag="EYO68_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07042.1"
                     /translation="MTIEVRNVCKSFKDVDAVLNVNLRVESGEAVALLGPNGAGKTTL
                     VEMIEGVQTPDSGEILILGKTWQSDESFLRQEIGLALQETRFQDRLTVEETLRLFASF
                     YHLEKERTNEVLKIIQLESKRKTWVVQLSGGQRQRLALGIAILSRPRILILDEPTTGL
                     DPNARRDLWKILEDFKATGTTLILTTHYMEEAEFLCERIVMMDQGKILAEGSLSQLLE
                     KFSEGDLIEYAIDGTFPIERAKALSGVVSSHWNAESGQGLLTVKSISETLPDFLDLIK
                     FSTASLTSLECRRRTLDDLFLAMSGRHLDE"
     gene            17267..18376
                     /locus_tag="EYO68_04215"
     CDS             17267..18376
                     /locus_tag="EYO68_04215"
                     /inference="COORDINATES: protein motif:HMM:PF12698.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07043.1"
                     /translation="MNKMLFRLVISQFKLFFREPGVVFWSFGFPVLMAWILGIAFANK
                     GEIERNIAVVAENPEEVYLVPEWLLNKTGTASTKYSRESGLEWKVGENHGEQAHFRFL
                     VMSEDAAILALKRGQIALWIEGSPERDLRFRFDPNNTDARLTYLLLESAFRKQKSLNR
                     KTEVLPLTTIGIRYVDFLIPGLMAISIMNACLWGIGWNLIELRIKKLLRRMVATPLKK
                     SVFLLSHGLNRMFISAAELLLLYVFAHFYFDITIQGSLLALSLIFLSGYSAFAGIAVL
                     ISSRAENTQSGNGLINSVTLPMFILSGIFFSYHNFPDWLIPYVEVLPLTILTNSLRGV
                     ITEGIGLAQAIQPAIGLFGIGGVCFALGLRLYRWH"
     gene            complement(18463..18870)
                     /locus_tag="EYO68_04220"
     CDS             complement(18463..18870)
                     /locus_tag="EYO68_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07044.1"
                     /translation="MAFKTKVVLVVLLAALLIGVPPGLGQQPPADNRGNLYSIWLKLS
                     MMGHNQSEIEGILTGITEQQLQRLKNRLRRDVLETLMHHNLHNEIELSRTEQDLVMIR
                     DIIRTEIRFAGLENDRLLQRMIRHKFGIALQNI"
     gene            complement(19309..19929)
                     /locus_tag="EYO68_04225"
     CDS             complement(19309..19929)
                     /locus_tag="EYO68_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIC07045.1"
                     /translation="MLNGLLARKIGMTQVFEEDGTALPVTVLQSGPVTVVQKKTLEKD
                     GCNHVQVGFEEIPERKVNKPIKGHFKAQAPTRILREFEVENIDEVEVGQVFDVSLFSE
                     GESITVSGNSKGKGFTGVMKRHGFHGQPASHGHRGHRGTGSIGQCATPARVFKGKKMH
                     GRHGNSKVTQLGLKIVKILADEAIVLVKGAVPGPNGGLVTILKSNR"
     gene            20234..21316
                     /gene="mltG"
                     /locus_tag="EYO68_04230"
     CDS             20234..21316
                     /gene="mltG"
                     /locus_tag="EYO68_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIC07046.1"
                     /translation="MKLFITNKQKLLLAGLFILGVSCMVGVAGYIYQIYYSPIMSSAP
                     DGNSVILIPRGSTFDSVTARIRKKGLLPYPRLYDYLAEKLKVHSRIQAGEFKIQHNWN
                     TSELLQYLISGKSIRHRITIPEGENYRQIAERLQLAEIADKDVILSLKDDPELLAKTG
                     IPDMTTLEGFLFPETYFFSRVDTERQILSAMITQYRRVFNADFQKRAKEIGMSEYKVL
                     TLASIVEKETGVDSDRPKIASVFHNRLKRRMRLDSDPTVIYGLSDFNGNLTRKHLRKP
                     TPYNTYTKYGLPPTPISNPGRASLQSVLYPADKKFLYFVARGDGSSKFSKTLREHNKA
                     VSYFQKNRKNRQAMRRKAKKASSKKL"
     gene            21321..23174
                     /locus_tag="EYO68_04235"
     CDS             21321..23174
                     /locus_tag="EYO68_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07047.1"
                     /translation="MSSAQSIQLGDSSFIPLLKMPPKTSSKFALLGYYIRQHLFSYSL
                     GVVAILGTNWIAVSIPEYVRLSIDLLNNDLKSRQDLLWEYMFIMLGLALVMVVVRTLS
                     RILFFNPGRAIECQIKNDMFRKLMALQKNYYDKNPSGNIISRINNDITGVRMICGFGL
                     MQAFNIITALSMTPFKMWQLSPNLTLYCIIPIIIVFTVVRLGMNVLVRNLRARMESLQ
                     RLSGFTVSSLSAIDLIKNQTMREWSTGRFEKENQDMLRRSMKIAWTRSFVMPMLSNLE
                     HFLKLLVLLVGGIMVIEEGFTIGQLTAFIAYSALLTFPLMGLGWVTTMFQQGLVGLTS
                     IQTVLQQDLPRANLISLPEKKLKSLFAEGGLKVNNLSYHYPESKKDVLRHINFSIHPG
                     QVIGVLGRIGSGKSTLVNGLNRYLDMGKGSIYLGDQDLATLSDKDVRTCIRTVTQLPF
                     LFSDTTENNINFALGNGDSDDRELFQKILYSAALDKEVARFPKQEKTVVGEKGIMLSG
                     GQKQRISLARAMMEPCDLLILDNVLSAVDYETERHLLREIHKLLKPINNQQALAGSLL
                     IVSHRVTSMEHADLILVLDEGQIVDQGQHADLIQRPGYYQQMWALQNDHNS"
     gene            23144..24745
                     /locus_tag="EYO68_04240"
     CDS             23144..24745
                     /locus_tag="EYO68_04240"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC07048.1"
                     /translation="MGTAKRPQFLNSVENPSHFETQHPTLKTRYFPLLSLKNVSNRLW
                     DKQILKDISWSTEFGQSWAILGPNGAGKSTLIKVVLGQIPYCGTIKRHQEIEDFGKIA
                     HVSLEQQKILVAREEKKDRYEEYSGNEEHFLTGQEVMDPEGKHPESLLKIAEQLGLSS
                     LLGNPLRYFSNGETRKTLIGKALLSEPKLLILDEPFEGLDTESVLWLKGALSDLIKNG
                     LAVWLVSHRFEELVPEITHVLCLKSGEIFAQGLRSQVLITEVMEGLYEKADHKETNLK
                     TNENSAEINENLIQTDFFESVPEKDRKHSPIRMRNVNVHYGEKVVLKNFYWSVEQGEN
                     WKIVGPNGSGKSTLLSLICGDNLQAYANEIYLFGRRRGTGESIWDIKQKIGFVSSEFQ
                     VRYRQPVTALKVILSGFFDSIGYYQPASVTQKERAVSLMELLEITNLADLDFTLLSYG
                     QQRLILIARAMIKSPPLLILDEPCQGLDRTNRNRVLELVDLVGQNTTTQILYVTHAAA
                     DHLNCLQRELCFEATPEGIFRPIVT"
     gene            complement(24778..25938)
                     /locus_tag="EYO68_04245"
     CDS             complement(24778..25938)
                     /locus_tag="EYO68_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIC07049.1"
                     /translation="MSYSGNVHEISFDEKQILLIGTAHISQSSVDEVNSVIDQEKPDT
                     VCIELCSSRHQAMLDKDQWKNMDIYKVVREGKSFLLFANLIMTAFQKRLGSQLGVKPG
                     AEMLEAANAAERVGAELILADRDVKITLQRTWRGMTFWGRMKVLSQLLASLFVREEIS
                     KEEIEKLKESDALSEAMQMLAEQSPEMKRILIDERDQFMAEKIKLAQGKRIVAVVGAG
                     HVKGLTAELEREHNLAELETVPPPGKIGTWLKWGIPTLIVGLVVYGFFTVETDVSIEM
                     IQRWFLINGTLSALGTAIAFGHPITIATAFVAAPFTSLNPAVAAGWVAGLVEAFLRKP
                     QVRDFESLADDITHLRGFWQNNITRILLVVMFANLGSAIGTFVGGFAIASLL"
     gene            26025..>27736
                     /locus_tag="EYO68_04250"
     CDS             26025..>27736
                     /locus_tag="EYO68_04250"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01435.16,HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07050.1"
                     /translation="MFLPFLPLILASAGLTLFSGELERTEPWLNLPLAVNLSLIILFS
                     FLLAQMPQWLRQFSKLKRFPEVRKGSYSSTKFSRPRTLILIGWLALVYGEHLDLRIGH
                     LFNNITEAESVSFGVLLLLYWLADAVAAIPVYQWNAHGLEEKIKKSVLHLRLQLPVLA
                     LIIIQTVWFWITSKFLLSFTSNWSLIFELLCSLILMVLVAPVVFVKSWGAKAIENGND
                     FEEIRKELENSRTPVTAILSWPDSIMPYSTAGVIGFVRGFRYLLISPQLLKSLSATEL
                     RAVTAHEAGHLRKQHLLFYLLAFICLLELFAFAGSANLLLTWTGVLEVSGMLMGVASI
                     LSIILFIRFGIGFLSQNFERQADCHAFERHGISPISTALMKVSLLNGINPEQDNWHHY
                     GIQQRIDFLSICLKKPEMLQKHHRRVFRIKLVCAVLLVGLLGANYMLSSDTLKIKVLA
                     WKLEQSADNWQLKDAPMLTKMGDLLYFQDQKTEAELWYRRALEMNPEESHTLNNLAWL
                     LTEKHNNDKKRLRESIELAQKASTLKQAAFIWDTLAEAYLINGKYEAAADAARQALKL
                     AKAK"
BASE COUNT         7704 a         6128 c         5819 g         7885 t
ORIGIN      
        1 gtacgcatcc attcaattgc taccgaaccg caacctgcgc caacgtccca caaaagatga
       61 tcaacaaccg gcatcaattt agataaagcg accgccctaa tttcgcgctt tgtcagttga
      121 ccgtcgtgta aaaatgcttc gtccggaagt cccggatttc tactgaggac atttgcgtta
      181 ttttcaggaa tgcattcgat ggccatggcg ttcagtgcag caccttccgg atgattccat
      241 gagtcagctt gtcctgaaaa ttgcctctcc ttcctgccgc ctagatgctc caaaaccgtt
      301 attatgcttt tgccaaagcc tcttgcgcaa agcagttttg ctgcttgttc cggactttct
      361 tcacccttac tgaggacgag taattttgcc cctggctgca cgaatggatg cagcatttcc
      421 aacggacgac catgaatcgt cagtgtttca acatccggaa gtgaccaacc aattcgtgaa
      481 cacatcagcg aaaaagtggt caacgcaggc ttgatccaga tttcttcaat cggcaaatgc
      541 cgcaacaatt tggctgcaat tccataacac aacgggtccc cactagccat gatcgctact
      601 ttttgagcgc ttgcagagtc ctctggtcgc caactgacga tttttgaaac tgcctcgcgt
      661 atcggtgaag accaaatgat acgttctcct tcccagtctt tcggaagcat cttcagatga
      721 cgctctcccc caagcacgat ttccgattca tagagcaagt cacgtgcatc ggcagataaa
      781 ttgtcccagc cgtcttcacc aattccaatt acacacagcc atttttttga aatatcaggc
      841 attttaactc tgaatattaa tgttattagc cactgatatt tcagctttct ttgctaaagc
      901 attaaccgcc gccgctgcca ttgcgctgcc gccgcgtcgt ccggatagtg tcaaaaaagg
      961 cacggctccc acgcattgtt ttacgagcaa ttcttttgat tccgcagcac cgataaaacc
     1021 aacggggaat ccaaaaataa cagctggagg ttctactcct tctgctagta tttccagcaa
     1081 ccgaaacaga gttgttggtg cattgcctac ggctacaact gcaccggcaa gttgtggcct
     1141 ccaaaattcc actgctgccg ctgagagtgt tgtttccatt ttgtctgcaa gtggagcaca
     1201 ttccggatgg ttattccaac atttgacttc gttatttgca gacagacgcg aacgaataat
     1261 tccttctgcc accatcctgg aatcgcacaa aacaggagcg cctttaagca gtgcggcata
     1321 tcctgcttgt gcggcgtccg gagtaaaaac cagatcctcc acaatatccg gcattccgca
     1381 ggcatgaatt aaacgtactg tgaggtcagc aatatcttcc ggtagttttg ataaatccgc
     1441 ttccttgagg attgttgcaa atgattgacg ataaatttct ttcggatctc gaagataatc
     1501 ccctgtcatt cggcaccaga ctttccagat ttttcttcca aagttacagg acccaaagga
     1561 tgatctgcat gtgggtatgg atggtgtcca tggttgtggt tatgatcgtg tgaatgttcg
     1621 tgaacatgag aatgtgcatc ctcgaccgca tctgagcttt cgtccgtccc tgttccaact
     1681 ccttcaacgt ggtgatgatg actttcctgg cgcagaccaa cttcattttc aaaacccaaa
     1741 aactgctccc ggtatttgca aagctggcag ttcatgttgt tttcaccatt tctgatttct
     1801 tcaacccgct ccgcaaatgc gtccaaaacc agcggatgat cgttaagata agaagctttg
     1861 atgaattcta tttccggatg tttggctgcc acaatgtcag tgtaatggta aatgcgttga
     1921 acgagaattc cggtaaacaa aaaatatgga aaaactacta tccgtttgta acccaaacgg
     1981 gtcgcgtgtt ccagtccggg ctctaccaga ggaaaagtca ctccggaata agccgtttcc
     2041 gcccagccaa gtccaagtcc ttcccaaagc atccgggtca cttttgccac attagaattt
     2101 gcgtccggat cagaagcgcc gcgtccaacc acgaccaaca aagtatcctc acgagaaact
     2161 ccgtctccgg ctttctgcaa agcatcttca acacgttcgc cggctgcgcg aatcagtttg
     2221 agatcgattc ccaattccct cgcatacgaa atttccaatt ccggattttg tgcttggtag
     2281 gcgtttaata cggaaggtat atcgtttttt gcgtgacctg ccgcaaacag cattccggga
     2341 accgccagga ccttgcgaat acctttggcc ctcaacgcat ccaaacctgt acgaatcact
     2401 ggagttgcaa attccaaaaa tccccaatcc gtttcatatt gcggcagtcg ttctttaagt
     2461 ccctgcgcga ccgcttgaaa ttcttcaact gctcctgtat ctcgacttcc gtgtccacag
     2521 accattacgc cgtattgctc tttcatatca ctcagttagt tttgatagtt tcgcaaaagt
     2581 ctgaaatcca cacagacatt tctacttagg taaaacatta atgatattag taacatagtt
     2641 tatgccagat ttctagttta gctcgaaatg aaacggtttt aggacttttt acaaagatat
     2701 cattattggg ttaatctatt aatacaccat cttaacagaa ttgtgattga atgagtgaaa
     2761 tttgtccaac ctgaaggaaa aaaatactcc tgaataagtc aaacaaggag tgacaaatat
     2821 ttttttagaa tttccctaaa tgtcttaatc catcataaac cactaccgcc accgatgagc
     2881 caagatttaa acttcgcacc cctttttccc acattggtag agtaaaacag cgctcaggga
     2941 aacgttccat ccattcatcc ggcaggccgg aagtttcttt accaaaaaac aagtaatcgc
     3001 cttcctgtgg tcgaattttt gtgtagggcc gctcattatg gacagaaagg aaatgaaagt
     3061 tttccggaac aagtgttgaa aaaaaatctt ctgcatcagg aaaatggtcc cagctcaccc
     3121 attgccaata atctaaaccg gcacgtttga gttgtttgtc gcttaaatca aaccccagtt
     3181 ttcccagtaa aatcaaatga cattttgtgg ctgcgcacag ccgcgcaata ttgcctgtgt
     3241 tgggcggaat ttgaggttcg tagagaacaa tattaagagg tggatttttg tgaaggcgca
     3301 tcagactttt atgggatgaa gcaaaataac tgcattctga actgcttttt actgtttatt
     3361 ccaatgcaag ttcaatcagc ctgtccaaca gttcggggta agctaatccg gatgcatccc
     3421 aaagtttcgg gtacatgctg atattagtga atccgggcag ggtattgatt tcgttaagga
     3481 tgaaggtttt gtcttcacga agaaagaaat caactctggc catgctgcgg cattcagtga
     3541 gcagaaatgc ctccactgca accttccgga tttgcatgga aacgtccgga tcaatttcca
     3601 caggaatgat taactccgca ccatcttcat ccacatactt tgcttcataa gaatagaact
     3661 cacgtagcgg aacaatttca cctaacaccg ctgcagcttc cggagaatcg ttgcccaaca
     3721 cagagcattc aatttctcgt ccgtaaattg cttcttcaac cataaccttg cggtctgctg
     3781 caaaagccat ctctaaggcc tgcgtatatt cctttacggt ccggaccttg gaaacgccca
     3841 gcgaagagcc ttcccgacaa ggttttacaa atagtggctc acccagttcc tccactgcat
     3901 ctacaaaaga caattcatcc ggattccgaa taagtttgta acgcgcaaca gtgattccac
     3961 gctgcaacag caaccgtttt gacacatcct tgtccatgca aattgctgaa ccataaacat
     4021 ccgcaccaac atacggttta ccaagcaatt ccagaaagcc ctgcaatgca ccgtcttcac
     4081 caagttttcc gtgagtgatt ggaaaaaaca catcaacatt agctagctcg tttccggact
     4141 gttctgttga tgccaggttt tcgccagcgg agtttccggt ttgaggaagg aggatcggta
     4201 aattttttct gaacttaact tgacgaacat catcgacatt gtccaaaaat tccggagttt
     4261 ttcccaaaaa ccaggcacca cttttatcga ttccaattaa gagtacatca tatttgtccc
     4321 ggtcaagagc agcttgaatg ttataggccg aaattaatga tacttcatgt tcaccactgg
     4381 tacctccaca aatcaggccg attgttagtt ttttggacat aactatggtt cacttaaaaa
     4441 gtgttttttg tcacccctgt tttgattcgg gatccaaatc aatgatcaga ttgatcacag
     4501 tatttaaagt atctaatctg gattcctggt cttcgctccg cttctcacgg aatgacaatg
     4561 aatcggaatt gaaaaatatt atttttcagc aagttctaac ttctcagttt gctgagccga
     4621 agccttgacc ctgatatgca gttcatcgag ttgctctgtt tcaactgcag agggtgcatc
     4681 agtcattaag cagccggctt tttgagtttt aggaaatgca atcacgtcac gtatcgaatc
     4741 tgtttttagc aaaaacatca tcatgcgatc aaacccaaga gcaataccac catggggcgg
     4801 agcaccataa gaaagtgctt tcatcaggaa accgaattta ctttcaattt cttcatcagt
     4861 taatttcagc aaacggaata ctttattttg aatatcctca cgatgaatcc gaatacttcc
     4921 accaccaatt tcaactccgt tcaataccac atcataggcc cttgagcaga ttttttcagg
     4981 atcctgctct ccgtattttt ccaaatcatc tatatccggc atggtgaaag ggtggtggct
     5041 ggaggtaaaa cttttttcag tttcactata ctcaaagaga ggaaaatctg taacccagac
     5101 aaaacgatat tcgctgttgt ttatcaagcc tcgacgtagc gcaatttctt ttcgcaaatt
     5161 accgagtgca tcatgaacaa cttttgtgtt gtcggcacag aacaacacta gatctcctac
     5221 attcaaaccg actctctctt ccaatgcctc tttttgttcg gaagtgaaaa acttaccgat
     5281 aggcgactgc caaccgtcct ggttgcgttt gatccaagcc ataccttttg ctccataaat
     5341 ttgagcgaat tctgtcaggt catcgagatc tttgcgcgaa aaatctgctc cgccaggaac
     5401 acaaatagct tttacaatcc cgcccttgtc caccacctgt ttgaaaacac gcaattcaca
     5461 ttcagaggca atgtcagaaa tatctttgag ctttaaatca aaacggatat ccggagcatc
     5521 ggaaccatag ttttccatcg actcctgata cgtcatgcgc tggaaaggcg tttcgacttt
     5581 tacacttatg gtttcttcaa acagcttaac catcaaacct tctgtcagct tatatatctc
     5641 ttccggttca gtaaaactca attccaaatc aatttgggta aattccggtt gacggtttcc
     5701 acgcaaatcc tcgtcacgaa aacaacgggc aagctgcatg tagcggtcat aaccgctgat
     5761 catcagtaac tgcttgaaaa gttgcggtga ttgcggcaat gcgtaaaaaa gtccgggatt
     5821 gactcttgaa ggcaccagat aatcacgtgc gccttccgga gtagattttg tcagtatcgg
     5881 agtttcaatc tcaaaaaagc gctgacttgt gaagtaattc cggacaattt gcaaggactg
     5941 cgaacgcagc attaaattat tctgcaaagt ggaatcacgc aaatcaagaa atctgtaagt
     6001 gagccttaat ttttcatcca cattttcacg attttccagg acatagggtg gtgtttccga
     6061 tttgttgaga atctccaaag tctccaccat aatctcaatt tcgccagtcg gcaattttgg
     6121 atttacgttg ccttctatac ggtgtgcaac ctttcctttt acagccaaaa caaactcacc
     6181 acgaattgaa tctgctaaaa catgtgaggt ttcactgatt tccattttga acacaatttg
     6241 gctgatacca gtgtaatccc gtaaatccac aaaaatcact ccaccatgat cgcgccgggt
     6301 ttgacaccag cccattaata cgacctcctg gtcaacattc tctatcctca aatcaccaca
     6361 ataatgtgtt cttgtccact ccattatttt attcatttta ttttagaaaa ttaatttcga
     6421 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcatcata aattcacccg
     6541 caaatttata tccagacatc gttttccgca gttagttcgc cgccggaatc acctgtattc
     6601 accacacctc ttggttcaac aagtagaatt ttacactcgt tgttagcaac aggtttgtgt
     6661 tcaatatttt ttggaataac aaacatttct ccagctttca tttcaacact tccatcacga
     6721 aactcaatct ccatttctcc ttccagcact ataaatacct catctgtatc gtcatgctca
     6781 tgccagacaa attctccttt tattttaacc aatttgaatt gatagtcgtt catttcggca
     6841 atcactcttg gttgccatag atctgaaaat ttattcagtt tttctgagaa attgatcgat
     6901 tgatagttca tcaaaaactc ccttttataa ataattatta atagctgcta agcgttggat
     6961 tgtagcataa ctgtatttca atcaacagat ttttctctat ttacaataaa caataatagc
     7021 aggctcagag tagattcagg taaatatttt gacttggcta agaataagtc aaccttttaa
     7081 tatctcggac ttaatacgtg agtcaactga cgaattaact ttaagataag atttgaataa
     7141 cacccttgaa ttccggagct aaagtgcttc tttaagattg aaaacggggg aaataaaaaa
     7201 tggaaattat gggaagaggg gattgtcttt tttgtattaa gttttgtgtc gccttaattc
     7261 aaaacaggag taaagtgatc agacctgcat acgcatcact tcttcatatg cacttaccag
     7321 tttgttgcgg atttgcagca tgaggcgcaa agagaggtcc gctttttcca gggcaagcat
     7381 ggttccatga atatctttat tgtctgtgac cagcaactca acttgttttg cttccgcgtt
     7441 cagttgctgc tgattgacat cctgcagcag tttctcaaac gtccggctga tgccgctttc
     7501 aacagactgc ttttgacttt cagcttcagt cttcagcccc tgtaaagctg gtccaactga
     7561 tggtaaattg atattcataa agtctctaat ctaacatcta ataaatcaaa ttgagcaatt
     7621 atttattatt actacgacga tctactctaa acgtttcggc ccaaatcgag ggctgattgt
     7681 gccatatttt ttgtggcgtt aaaagcagcg gtatttgctt cataggaacg cgaagcctcc
     7741 atcaggctca ccatttcttc cattatgttt acgttaggca tggccacata tccttcttca
     7801 tttgcatccg gatgtcccgg ttcataacgt aaaatcggtg ctctgttgtc ttgtgaaatt
     7861 cccaccacct ttacctcggt agcagtgtcc agagaacgtt gctgtgagcg cagttcttct
     7921 tcaaatgtcc tgtcattgga aactgctgca agcgtaacta tacgacgacg atacggcccg
     7981 ccttgcggag tccgtgtagt actggcgtta gcgatgtttt cggaaatagt attgactcgc
     8041 tggcgctgcg ctgccattcc ggaaacactc acattaagag aggattgaaa actcattatc
     8101 gaccttctgt aattgcggtt ttgagcatcc ggaactggtg agtcagcatc cgtatactgg
     8161 cattgtacat caattggttt tcagccattt tagccatttc acgatccaaa tctaccgtgt
     8221 ttccgtcaaa atcaggaaaa ggagaagcgc tgtgaattcg ctgtgcctga accagtttca
     8281 gcggcggcgt gttgcgtcca tcaaaatgcc tggaatttgt ggttttgagc aaacctggtt
     8341 cttcggcatg cagtgccttt tcaagacttt tctcaaatac catatcctcg gctttatagc
     8401 cgggagtgtc cttgttggca atgttcgaag tcagcaaatc ctgtcgctgt gtgcgaaagt
     8461 ccagtgtctt ctgaatcaat gtaaacagat tattttcaaa cattcctgac atgaaaccac
     8521 ttatttttgg atagtattca actaataata tttgtgatga agatgcaatc tcagggccac
     8581 ttttagaatg tatttgattg ttatagccag agtgatctgc ttgcattttt tctgattcgg
     8641 cttaagatag acaaacaaag ttttaatcac gaggaaagat ggataaaaaa cgagcacaag
     8701 tttggggaag tcaccttaaa aaaccaccca acgagcagaa catccttttc tgcgcaggac
     8761 gggatgtgca gccattacca atggcagatg agttgctgct gccctacgat atctggacca
     8821 accgcgcaca ttgcataatg cttcacaagc agggactgat accatcagca agcttaagca
     8881 gtattttggc cggacttaac aaactggaaa aactggttgc ggcaggtaat ttttcacttg
     8941 atccagccaa agaagatgtt cacatcaacg ttgaagcctt tgtaacagaa aatcaagggg
     9001 ctgaagcagg cgggcggatg catataggtc gcagtcgcaa tgatcagtcg gcatgtgaca
     9061 tgcggctgta tttacgcgct gtgggcttaa gcttgtttga ttctgtgaaa aatttagcag
     9121 cctcattgtt ggtacaggca gaaaaaaata cagaaagtgt aatgccagga tttactcatt
     9181 accaacctgc gatggtcaca acatggggtc actggatgtg tgcttatgtt cagggacttt
     9241 gccgtgacct ggaacggttg gcatttgcca tctcactagt taatcggaat cctttgggag
     9301 cagcagcagc ttttggtaca tcatggccca ttgaccggca actcacaact gagcttctgg
     9361 cttttgaaaa gattgatacc aacactctgg actgcatcgt ttcacgctgg gaaaatgaag
     9421 cccaactggc ccatgcagga tcaatgctga tgaatcacct cagtattatg tctcaggatc
     9481 tcatgttttt aagtcatcca tattcaggat tgcttcagat tgatgatgac tatgtgaccg
     9541 gttcttcgat catgccgcaa aagaagaacc cggatttcgc cgaagtcatt aaatcaaaag
     9601 cagctctagc ccacggattt ttatcaagct tgcttggaat tcaaaaaggt ggattgagcg
     9661 gctataaccg tgacacgcag gccactaaat atttattaat ggaccttgta cgggagtgtg
     9721 aagctgcacc aatcattctg cagggtgtct tcgaaagttt gacaatcaat accgaaaaaa
     9781 tgcgggaact gtgtgaaacc gactttatga attctgtgga tattgcagat catttggcac
     9841 ggactctaaa cctgtctttt cgagagtgct accacattct ggcgagggca gtaaaacttt
     9901 ctttgccgga aacaaaaatt acaactgctg cattgaagaa agcattggca gaatccgatc
     9961 atccaacaga aattgcagaa gatctggcag aattacatga tcctcaaaaa attctgaaac
    10021 tcagacagca ccagggtagt ccgtccccgg agcaaaccag cctgcaaatt gaagaacttt
    10081 ctgcagaact gaattcactg tctgctccgc ttgttgatct gcaaaacaat attattgctg
    10141 cacaggagaa ctgccaaaat tatggggtct gatggtttaa aaattggcgt gattggtgct
    10201 ggggcatggg ggacatccct ggctcggctg cttgccagca gcggcaactc cgttcaactc
    10261 tggtgccacg aacctgaaac tgcaaaaaac atcctccaga aacacgtcaa cacctctttt
    10321 ttaccggata ttctacttcc ggaatcactt ggagccagca ctgatttgct taaaatcgtc
    10381 caaaattccc gtattctcgt cgctgcaact ccttcgcact taacccgcga aattgccaaa
    10441 aaaatacgtc cggagattac tgccgaacat attttgctaa ttcttagcaa aggaattgaa
    10501 cagcattccc tggcattgat gagtgaaata tttgcggaag aactcgacaa cctgccgaaa
    10561 ttagcagttc tttccggccc gacgtttgca cgtgaagttg ctttggattt gccatctgcc
    10621 gcagtgctgg cctgtgcaga cgaaaccgca ggcagtctgc ttcaaaagtg ctttcattca
    10681 gatcgcttcc gtatttaccg cagtactgat cttgttggcg tacaactggg tggttccatt
    10741 aaaaatgtga ttgcgattgc ttccggaatt gcagatggaa tgcaactggg actgaatgca
    10801 agggcagcct taatctgcag aggcgttgca gaaatgtccc ggctcggaac tgcaatggga
    10861 ggatctgcag aaacatttat gggtatgtcc ggaattggag atttggtttt gactgcaacc
    10921 gggaatcttt caaggaacca taatcttgga gaaaaactgg gccagggttt ttcgctggaa
    10981 gactgtcttc catcaggcgg tgcggtggca gaaggggtac gtaacgcggt ttcgattcaa
    11041 gaattggcag agcgccacaa aattgaaatg ccaatctgta acgcagttta tcaagtattg
    11101 catcaaggag tttcctgtac tcaggccttg caaaaacttc tggaacgtga tcgtccggat
    11161 gaagaaatgt atgtcccgga tttagctaca atgccagatt aacaaacaag tttgaaagtt
    11221 agattgttaa gacagtatcc taatttgtta atttttataa agccatgaac agtttttttt
    11281 ctactaaaaa ctggaataaa ttcttcctct atctcttatt catttgcagc actttcagca
    11341 ttgccggtac tgatgcctca atcacaatcc tgtatttaat gaccctgata agctggatct
    11401 ggtaccgcca aatgtcacaa ctcaggaatc cactgttatg gtcaatttta tttttcatgg
    11461 ctgccgcagt actttccgga ttgctgaatg catacgaaac agagcacctg atggctctac
    11521 gtacaaattg gcgcttgctg ctgcccttat tgcttgcggt aatattggct gatattgatg
    11581 aagaacagtt actgtcggtt ttctttggat ttgtaatgtt aatctcaatt tacggcatca
    11641 ttcagtattt taccggtgca gactggctgc gtcctgaagg gcaacaatta actactcctt
    11701 tttcgtcagg aacttcgaca gaaagtcccg tttttcacgg taaaggaaat ttctcgcacc
    11761 acctgacgta tggtggtttt ttactacttt gttttccttt ggtttgttca ttcatttttt
    11821 gtaaaggctg gagtccattt gcccgtcttg taacggccat tattgcagtt gtggtactgc
    11881 ttggtatagg agcctcactt ggtcggagca tttggctggg aactgctgtc gcaatcacta
    11941 ttctactttt tcgactttca cctaaactta tgctggcctt cagcattctg gttatcgcag
    12001 gtggcactta tttttactca caatttagtg agccttcttt tcaaaaccgc acaccaacaa
    12061 atcgtatcga agccctgcag cagcgcttca tcagtggctt catgccccaa tctaaccagg
    12121 atcgtatttt aatgtgggaa gcaggaatat ctgcgataca ggatcatttc tggttgggaa
    12181 tcggttatgg taatgactca gaaatcatgc ccgtttacag ggaaaagatt tcagagcgta
    12241 caggtcatcg attcaacaac agcgccggta cagggatcca taacatctat ctgcaaactt
    12301 ggattaacta cggccttttc ggttttttgg gataccttag tatcttgatt atatttttct
    12361 ggcaaagcat cttaacactc taccgaacca ctccctacag ctttgaaaat tcaatcctct
    12421 gggctggaat tgcaggtgta agcggattca tggttgccgg tttttttgaa aacaatttcc
    12481 gggacggtga agtccaggct atgctcatga tactgatagg cttgaacctg caccaaattc
    12541 aaaaaatgaa agaacggata tttagtcatt gaaagcaaaa cagcaggcac ctaaatatcg
    12601 ttcctctggc aaatgtctca gggaaactct ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnaaacagca ggcacctaaa tatcgttcct ctggcaaatg tctcagggaa
    12781 actctctaca ctttcgttga acaactcctc caaatttcac tcaatatcct gaccagaatt
    12841 gttaattaaa acacagacaa actctcacta aaaactatgg gattaattat tatctctttt
    12901 tttgcctttg tttccctggg cttaccagac ggactcatgg gcgtggcatg gcctgggatt
    12961 cgtcaccaat tccaactgcc agttgatgct ttaggaatta tccttgtctg cggaacagca
    13021 ggatacatgc tttcaagctt tttgagtgga ctactgattc gacatttagg aatcggtggg
    13081 ctgctgagtc tgagttgtgc tgccatggct ggtacattac tagtttatgc agttactcct
    13141 ctctggtggg ttttcgttat tttttctgca gttggaggac ttggtgccgg tgcaattgat
    13201 gccggaataa atacctatat cgccaaatac cacagtacca gaatgatgca gtggttacac
    13261 gcaagttttg gcgtgggaat tacctccggt ccattcataa tgacaatggg aataaacgca
    13321 acatcccatt ggcaaagcgg atattttgtt gtttcagtgg caatggtaat tctttccgta
    13381 atttttcttt ttactaaaga catgtgggaa ggtatttcag ccagtagtag agaagaagat
    13441 cacgatgaga ataatgcttc cctcagtgaa actataaaat caattcaggt actcttaagt
    13501 gtggcattgt tttttcttta tacaggtgta gaattgggtt tagggctttg gatttactca
    13561 cttttgacag aatcccgtgg agttgctccg gaagttgctg gaattgtaac cggaggttac
    13621 tgggcaatgt ttactgtcgg ccgtattttt gcgggatggt acgctaaaaa gatagacatt
    13681 cgcaaactaa tttacttgag tatctcactg acacttttcg gagtagctct gctgctggca
    13741 gattccagtc aaataataac aattcttgga gtcactttgg ccggttttgc gatcgcacca
    13801 atatttcccg gactggtttc cagcacaaca agtcgtgtag ggttccggca tcaggccaat
    13861 acaattggaa tgcagattgc cgcagcaggt ttgggggtgg ctgtggttcc ttcaattgca
    13921 ggtgtgctgg caagaatctt tggacttgaa gtaataccac tttatttgtt gagtacactg
    13981 gcactgctgt tattggtttt tatcatttcg aatagtcatt ccggtgagaa tacataggat
    14041 tgactgaaaa ctctcaaacg tttggactct tctgaaacta tgaaatttat caacatacaa
    14101 tattgtaaaa caaaaattgg tgaactgatt ttaggctcct tcgaaggcaa actttgtctg
    14161 cttgatttta gatacagaaa aatgagaaaa tccgttgatg aaagaattaa aaaaggacta
    14221 aatgccaaat ttgtagaaaa taacacggaa attatagaaa gaatccgggc agagcttgat
    14281 gaatattttc atggcaacag aaaagagttt gatgtccctc tacaaatggt aggaacggat
    14341 tttcaaaaaa gtgtctggga agctttactg agagttcctt ttggaactac gtcaacttat
    14401 ttgcagttgg ccaaaaatat aaagaatgaa aaagcggtca gagcagttgc cgctgctaat
    14461 ggcgcaaact cgatggcaat cataattccc tgtcatcgta taattggaag taatggagag
    14521 cttgtcggat atgccggcgg actgccgatt aaaaaacgat tgttaaagtt ggagcaaatc
    14581 acaactcaat ctggcactaa ataattttaa aaagaaataa ggtcatattg atacagttga
    14641 ctaagtttac tttattttcg gagttttcat gaacaacata ccacaatcaa tgaactacat
    14701 tcagatcgaa aaacatgggg atcctgaagt attaaaactg cattcgatgc cggttccgga
    14761 accaggcccc ggtgaagtac tgattaaggt cgcggcggcg ggagtcaacc gtccggacgt
    14821 gatgcagcgc aaagggcttt atccaccgcc tcccggcgca accgatgttc ccggactgga
    14881 ggtttccgga actgtagtga gtgtgggtca aaacgtcagc gagcccccca tcaattctga
    14941 agtttgtgcc ctcgtgacct gcggcggtta tgcggaatat tgtctggcgg cagcctcgat
    15001 ttgcttgccg gttccggaaa aaatttcgct agtaaatgcc gcgggaattc cggaaacctt
    15061 tttcaccgtc tggacaaatg tctttaaacg cggacaactc aaagccggag aaagtctgtt
    15121 ggtacacggc ggtagcagtg gaataggaac aactgccatt caactcggaa aagcctttgg
    15181 ggcaactgtt tacaccactg caggaacttc ggataaatgt gagttttgta ataatcttgg
    15241 tgccgatgct gccatcaatt acagagaaca ggatttctcc gaagaaatca aacgcttgac
    15301 tgaaggaaaa ggcgtaaatg tgatccttga catggttggc ggcccatact ttccaaaaaa
    15361 catccggttg ctggccgacg aaggtcggtt agtgcaaatc gctctgatgc agggcagcaa
    15421 agcagaagtt gattttcgct ccttactgct caaacgtgta accttgaccg gctccacctt
    15481 gcgtccccgc agcgttgaag aaaaaactaa aatcgctcag gccctgcaaa aaaacgtttg
    15541 gccattgctg gaatccggag ctatccgacc gatcatccat caaacatttc cgctcaaaca
    15601 agcgtcagaa gcacatcgcc tgatggaaag cagtgcccac attggaaaaa ttttacttaa
    15661 gcctgctgat tgacaccgat caaaaagtat tgttcgctgc ggaatatttc ctgctaaact
    15721 gccgaagtga atatgtgcaa aatccaagtg tctcgccaac ttaacttgac aaatctaact
    15781 catttctgaa acactgcagt ttcaactttt ttctattctt ctcaaaagga ggctttatgg
    15841 atgagaatgc taaaaaaacc gcattgcgga tgattcctta tggactttac gttatgacag
    15901 ccgaagacaa agatggtcga atttcggctg ctactgtgaa ctgggtcaca caggcttcgt
    15961 tcaaaccgcc cctggttgct gtcggtgtta aagctgattc ccagatacat gacattatta
    16021 aaacagccgg aaattttgca ctcaatgttt tgggcaaggg acaacagggt gcggcctatg
    16081 cgtttttcaa accggctgaa agagacggac aaaaaataag cggcgagcca tttcatgcag
    16141 gaagtaccgg agcaccagtt ttggagaaca cacctgcttt cgttgaatgc agacttgtaa
    16201 caactgtgga ggaaggagac cattccattt ttgtgggaaa agtcgttgat gcaggagtaa
    16261 cccaggaacc ggaaggtcgt gcagatgagg caactctgtt gctgaaggac cttggtgaaa
    16321 agacttttta cggcggctga tcgggaaaat tctatgacga ttgaagtccg gaacgtctgc
    16381 aaatccttca aagacgttga tgcggttcta aacgtcaatt tacgggttga atccggagaa
    16441 gcggtggcct tgcttggacc aaacggtgcg ggcaagacaa ctttggtgga gatgatagaa
    16501 ggcgttcaaa ctccagattc cggcgaaata ttgatccttg gaaaaacctg gcaaagtgat
    16561 gaaagttttt tgcgtcagga aataggcctt gccctgcagg aaacaaggtt tcaggaccgt
    16621 ttgacagtag aagaaacact ccggcttttt gccagttttt atcatcttga aaaggagcgt
    16681 acaaacgagg ttctcaagat cattcagctc gaaagtaagc gcaaaacatg ggttgtccag
    16741 ctttccggag gacaacggca gcggctcgcc ctgggtattg caattctatc ccggccccga
    16801 atcctcattc tcgacgaacc tacaaccggc cttgatccaa acgccaggcg tgatctctgg
    16861 aaaatcctcg aagattttaa ggcaactgga accacattga ttctcaccac acattacatg
    16921 gaggaagcag aatttctctg cgaacgaatc gtgatgatgg atcagggtaa aattctcgca
    16981 gaaggttctt taagtcaatt gctggaaaaa ttcagcgagg gggatttgat cgaatatgcg
    17041 attgatggaa catttccaat tgaacgggca aaagctcttt ccggagttgt tagctcccac
    17101 tggaatgcag aaagtggaca gggacttttg acagtaaaaa gtatttcaga aacattgccg
    17161 gattttttag atttgataaa attttctaca gcttctttga cttcccttga gtgccgcagg
    17221 cgtactcttg atgatttgtt cctggccatg agcgggaggc atttggatga ataagatgct
    17281 tttcaggctc gttatttctc aattcaaact gttcttcagg gaaccgggag tcgtgttctg
    17341 gtcttttggg tttccggttt tgatggcctg gattttggga attgcctttg ctaacaaagg
    17401 tgagattgag cggaatattg cagttgttgc agaaaatccg gaagaggttt acttggttcc
    17461 ggaatggctg cttaataaaa caggaactgc atccactaag tatagcagag aatccggact
    17521 tgagtggaaa gtaggcgaga accatggaga acaggcacac tttcgtttcc ttgtcatgtc
    17581 agaagatgca gcgatacttg ctctcaaaag aggacaaatt gcactctgga tcgaaggcag
    17641 tccggaaaga gatttgcgtt ttcgctttga tccaaacaat acagatgcac ggctgactta
    17701 tttactttta gagtcagctt tccggaagca aaaatcttta aatcgaaaaa cggaagtcct
    17761 gcctttgacc acaatcggca tccgctatgt tgattttttg attccgggtt tgatggctat
    17821 ttccatcatg aatgcatgtt tgtggggaat cggctggaat ttaattgagt tacgtatcaa
    17881 aaaattgctg cgccggatgg ttgccactcc actaaagaag tcagttttcc tgctctcgca
    17941 cggtttgaac cgcatgttta tcagtgccgc agaactgctt ttgctttacg tttttgcaca
    18001 cttctatttt gatattacaa ttcaggggag tctgctggcg ctgtctttga tttttttgag
    18061 tggatacagt gcttttgccg gaattgccgt tctcatttca tcccgagcag aaaatacgca
    18121 aagcggcaac ggcttgatta acagcgtgac cctgccaatg ttcatccttt ccggaatatt
    18181 tttcagttac cacaacttcc cggactggct cattccttat gttgaggtac ttccattaac
    18241 gatactaacc aattctttac gtggagtaat aacggaagga atcggactgg ctcaagccat
    18301 ccagccggct attggcttat ttgggatagg aggtgtttgt ttcgcgctgg gtttgagatt
    18361 ataccgctgg cattaagatg tgtaagtgtt gaatgttggg tttttcttga ggaccaaaga
    18421 gagcacacta taattcaaca ctcacacaat atgtcatttg ctttaaatat tctgcagtgc
    18481 aatcccgaat ttatgacgga tcatgcgctg cagcaggcgg tcgttttcca atcctgcaaa
    18541 acggatttcc gttctgatta tgtcacgaat catcaccaga tcctgttcgg tcctgctcaa
    18601 ctcaatttca ttatgcagat tgtgatgcat caaagtttcc agcacatccc gccgtaagcg
    18661 gtttttgaga cgctggagtt gctgctccgt tattccggta aggataccct caatctcaga
    18721 ctggttgtgt cccatcattg agagcttcaa ccagatggaa tacaggttcc ccctgttgtc
    18781 tgcaggaggc tgctgaccaa gtcccggagg tactccaatc aacaaagctg caagtaagac
    18841 taccagaacc accttggttt tgaatgccat ttttctcctt ttttctatta cgatttacgg
    18901 cttcaaagaa tggatatttt cccgcacaaa ataacgggaa actccgccct tcaacctgtc
    18961 atttcgataa actaagccat cacaaactgc gaaaccacac catgcagtac gcagttctcc
    19021 acaggcgatt gttcacaaaa ccggaacaat acgctgtatt tgttatgaca acaattcatc
    19081 tattatttac aagaacataa atcaggacca ccctgtggac acaccacgcc ccatagagtt
    19141 aagcagacgg aattccagac agaactctcc ctgccattcc aacggaaaag caatccaaac
    19201 ctcatagttc gttatcgaca gaacagccgg aaactttaag atgaatatcg gggaatataa
    19261 aaagacaggc gacacagaaa gcgccgccca cattatgctg ctaacaaatt agcggttgct
    19321 cttgagaatt gttaccagtc cgccgttggg accaggaact gcacctttga caagtacaat
    19381 tgcttcatct gcaagaattt taacaatctt taagccgagt tgagttactt tactgttgcc
    19441 gtgtcgacca tgcattttct tgcctttgaa aacacgggca ggggttgcac actgtccaat
    19501 acttcctgta ccgcggtgcc cacggtgtcc atgactggca ggttgaccat gaaatccatg
    19561 acgtttcata actccggtga atcctttacc ttttgagttg ccggaaacag tgatactttc
    19621 tccttcagaa aataagctta catcaaatac ctgtccaact tctacttcat caatgttctc
    19681 tacttcaaat tcacgcagga tgcgagttgg agcctgtgcc ttaaaatgcc ctttgattgg
    19741 tttatttact ttacgctccg gtatttcttc aaaaccgact tgtacatggt tgcaaccgtc
    19801 tttttccagg gtcttttttt ggacaactgt cacaggtccg gattgcagaa cggtcactgg
    19861 aagtgcagtt ccgtcttctt caaaaacctg ggtcatgcca atcttgcgtg ctaatagtcc
    19921 attcaacatg gagtcctcaa tcttaaaatt ctttattttt ttaaacgctc atgcataatc
    19981 ggagcgtttt taattagtgc acataaaata ataaatgatt tgtgaatgga atacaagttt
    20041 aattttatga agcatacata atccattcat ccagtgcctg tttcaaaaat gcagtaatta
    20101 tgaacatttc actaattcac aatataacag aaagtcaagc cccaaggttc aacatattgc
    20161 gttctgttct gtctgtcgat ataattgatt tcaatattaa caatactcac atttatctgt
    20221 atttttcaac tcaatgaagt tgttcatcac caacaaacag aaacttctgc ttgccggcct
    20281 gtttatacta ggggtttcgt gcatggttgg tgttgcagga tatatttatc aaatttacta
    20341 tagccccatc atgagcagtg ctccggatgg taattcggtt atcttgatcc ctcgaggttc
    20401 cacctttgac agcgttacgg cacgtatcag aaaaaaggga ctcctgccct atcccagact
    20461 ttacgactat ttggcggaaa aattaaaagt acattccaga atccaggccg gagaatttaa
    20521 aattcaacac aattggaata cctctgagtt gctgcagtat ctgatttccg ggaaaagcat
    20581 ccggcatcgc attaccattc cggaaggtga aaattatcga caaattgcag agcgcctgca
    20641 gcttgcagaa attgcagata aagacgtgat cctgtccctg aaagatgatc cggagttatt
    20701 agcaaaaact ggaattccgg acatgaccac tctggaagga ttcttgtttc cggaaactta
    20761 ttttttttcg cgggtagata cagagcgaca aattttatca gcaatgatta cacaataccg
    20821 gcgggttttt aatgctgatt ttcagaagcg tgccaaagaa attggaatga gcgagtataa
    20881 agttcttacc ctggcttcta ttgtggaaaa ggaaactgga gttgattcag accgaccaaa
    20941 gattgcttcc gtttttcata atcgactcaa acgcagaatg cggctcgaca gtgatccaac
    21001 agtaatttac ggactgagtg attttaacgg aaatttgact cgaaagcatt tacgaaaacc
    21061 aactccgtat aacacttaca ccaaatacgg attgccccct actccaattt cgaatcccgg
    21121 tcgagcatca ctgcaaagtg tgctttatcc ggcagacaaa aagttccttt attttgtcgc
    21181 acgcggggac ggaagcagca aattttcaaa aacattgcgg gaacacaata aggcagtttc
    21241 gtattttcag aaaaaccgca agaaccgtca ggcaatgcgt cgcaaagcta aaaaagcttc
    21301 ctcaaagaaa ttatagtccc atgtcctcag cccagtcaat tcaacttggt gattcctcat
    21361 tcattccgtt attgaaaatg ccgcctaaaa ccagcagcaa atttgccctg cttggttact
    21421 atatccggca gcatttgttt tcttattctc tgggagtggt tgctatttta ggtaccaact
    21481 ggattgctgt cagtataccg gaatatgtgc gtttaagtat tgatttgtta aataatgatc
    21541 tcaagtcaag gcaggattta ttgtgggaat atatgtttat catgcttgga ttagcacttg
    21601 taatggtggt ggtgcggaca ctttccagga ttcttttttt taatcccgga cgtgccatag
    21661 agtgtcagat aaaaaatgac atgttcagaa aactgatggc tctgcaaaaa aattattatg
    21721 ataaaaatcc ttcaggaaac ataatttccc gcatcaataa tgacatcact ggagtccgta
    21781 tgatttgcgg attcggcctc atgcaggcct tcaatatcat aacagcgctt tcaatgacgc
    21841 cttttaaaat gtggcaactc tcaccaaatc tcacacttta ctgcatcatt ccgattatca
    21901 ttgtttttac agtagtgcgc ctgggtatga atgtgctggt gcgtaatttg agggcccgta
    21961 tggaaagctt acagcgtctc tccggtttta cagtttcatc tctctcggca atagacctga
    22021 ttaaaaacca gacaatgcgt gaatggagca cggggcgttt tgagaaagaa aatcaggaca
    22081 tgcttcgacg ttcaatgaaa attgcctgga cccgctcttt tgtgatgccg atgttaagta
    22141 atttagagca ttttttaaaa ctcctggtat tgcttgttgg tggaatcatg gtaatcgaag
    22201 aaggcttcac cattggtcag ttaactgctt ttattgccta ttctgcatta ttgacatttc
    22261 ctttgatggg gttgggctgg gtaacaacca tgttccagca gggtctggtg ggactgacca
    22321 gtatccagac tgttttgcag caggacctgc ctcgtgcaaa ccttatttca cttccggaga
    22381 aaaaacttaa atctcttttt gcagaaggag gactgaaagt aaataattta agttaccact
    22441 acccggagag caagaaagat gtactaaggc acataaattt ctccatccat ccaggccagg
    22501 taattggagt actgggaaga attggttctg gaaaatcgac ccttgtcaac ggcttgaatc
    22561 gttatttgga catgggcaag ggttccatct atttaggaga ccaggacctc gcaactctca
    22621 gcgacaaaga tgtccgtacc tgtatcagaa ctgtcactca gttaccattt ttattttcag
    22681 atactacaga aaataatatc aactttgctc tgggaaatgg ggattccgat gaccgggaat
    22741 tgtttcagaa aattttgtac tcagcagctc ttgacaagga agttgcacgt ttcccaaaac
    22801 aggaaaaaac tgtggtcggc gaaaaaggaa tcatgctttc cggtggacag aaacaaagaa
    22861 tcagcctggc acgggcaatg atggaacctt gtgacctgct gattttagac aatgtccttt
    22921 ctgcagttga ctatgaaaca gaacgtcatc tgctgagaga aatccacaaa ctcctcaaac
    22981 cgattaataa tcaacaagcc ctggcaggaa gcctgcttat cgtttcccat cgtgttacat
    23041 cgatggaaca tgcagatttg attctggttc ttgatgaagg acaaatagtg gatcagggac
    23101 agcatgctga cctgattcaa cgtccaggtt attatcaaca aatgtgggca ctgcaaaacg
    23161 accacaattc ctgaacagtg ttgagaatcc atctcatttc gaaacccaac atccaactct
    23221 caaaactcgg tactttcctt tgctctctct caaaaacgtt tccaatcgac tctgggataa
    23281 gcagattctc aaagacattt cctggtccac agaattcgga caaagctggg cgattctagg
    23341 tccaaacggc gctggtaaat caactctgat aaaagttgta ctggggcaaa ttccatattg
    23401 cggtaccatc aaacgtcacc aggaaattga ggattttggc aaaatcgcac atgtttcact
    23461 tgagcagcaa aaaatcttgg ttgcccgtga agaaaaaaaa gaccgctacg aagaatacag
    23521 cggaaatgaa gaacattttt taaccggaca agaagtcatg gatccggaag ggaagcatcc
    23581 ggaatcactt ctcaagattg cagaacaact tggactttcg tccctactgg gaaacccttt
    23641 gcgctacttt tcaaatggtg aaactcgtaa aactttgatt ggaaaagccc tgctttctga
    23701 accaaaattg ttgattcttg atgagccttt tgaaggtttg gacacagaat ctgttctgtg
    23761 gcttaaagga gcactttcag atcttattaa aaacggatta gcagtctggt tagtcagtca
    23821 ccgttttgag gaattagttc cggaaatcac acatgttctt tgtttgaaat cgggagaaat
    23881 ttttgctcaa ggtttgcgct cgcaagtttt gattacggaa gtgatggagg gcctttatga
    23941 gaaagctgat cacaaagaaa ctaacttaaa aacaaacgaa aactctgcag aaataaatga
    24001 aaacctaatt caaactgact tctttgaatc agtacctgaa aaagacagaa agcacagtcc
    24061 tatccggatg aggaacgtta atgttcacta tggtgaaaaa gtggtgctga aaaattttta
    24121 ctggtcggta gaacaggggg aaaactggaa aattgtgggt ccaaatggat ccggtaaatc
    24181 gaccttgcta agtttaattt gcggagacaa tctacaggct tatgcaaatg aaatttatct
    24241 ttttgggcgt cgtcgtggta cgggtgaaag catttgggac atcaagcaga aaataggatt
    24301 tgtctcctct gagtttcagg tacgttatcg gcaacctgtt actgcgttga aagtgatact
    24361 ttcaggattt tttgattcaa tcggttatta tcagccagca tccgtaacac aaaaagaaag
    24421 ggcggtcagt ttgatggaac tgctggaaat tacaaatctt gctgatcttg acttcacgct
    24481 tctttcgtat ggacagcaac gacttatctt aattgcaaga gcaatgataa aatctccacc
    24541 tttactcatc cttgatgagc cctgccaggg attggatcgt acaaaccgga accgcgtttt
    24601 agaactagtt gacctggttg gacaaaacac aacaacacaa atactttacg taacgcacgc
    24661 cgcagctgat catttaaact gcctgcaaag agaattgtgc tttgaggcaa ctcccgaagg
    24721 catttttcgc ccaattgtta catgagtgtc tgcacatgga atatctctgc ttcccgatca
    24781 aaggagagac gcaatggcaa atcctcccac aaatgtgccg attgcactgc ctaaatttgc
    24841 gaacatgact acgagtaaaa ttctggtgat attattttgc caaaaacctc tcaaatgtgt
    24901 gatatcatct gcaaggcttt caaaatcccg tacctgaggt ttacgtagaa atgcttcaac
    24961 taatcctgcc acccaacctg cagcaacagc aggattgaga gaagtaaagg gagctgccac
    25021 gaatgcagtt gcaattgtta ttggatggcc aaatgcaatc gcagttccga gcgctgaaag
    25081 tgtgccgttg atcaaaaacc agcgctgaat catttcaatg ctaacgtccg tttcaacagt
    25141 aaaaaatccg taaacaacca accctacaat cagggttgga ataccccatt tcagccacgt
    25201 gccaattttc ccgggaggag gcactgtctc taattcagcc aaattatgtt cccgctccag
    25261 ttctgccgtc agtcctttta catgtccggc tccaactact gccacaatcc ttttgccttg
    25321 agccagtttt attttttctg ccataaactg atctctttca tcaatcagaa tacgtttcat
    25381 ttcaggagac tgttctgcca gcatctgcat tgcttcagaa agtgcatcgc tttccttgag
    25441 tttttctatt tcttccttgc tgatttcttc acggacaaac aaactggcaa gaagttgact
    25501 caagactttc attctgcccc aaaatgtcat cccccgccag gtacgctgca gggttatttt
    25561 aacatccctg tctgccaaaa tcagttccgc tccaacccgt tctgcagcat ttgctgcttc
    25621 aagcatttct gcaccaggtt ttactcccag ctgagaaccc aagcgttttt ggaaggcagt
    25681 catgatcaaa tttgcaaaaa gcaaaaagga tttaccttca cgaacgacct tataaatgtc
    25741 catgtttttc cattgatctt tatccagcat cgcctgatgt cgggaagaac agagttcaat
    25801 acaaactgtg tccggctttt cctgatcaat gacagagttc acctcatcca cacttgattg
    25861 tgaaatgtgt gcagttccga taagtaagat ttgcttttca tcaaagctaa tctcatgtac
    25921 attgccggag taagacattt actaaaataa gttttaggtt ttagaatcaa caaaagtcaa
    25981 taaatagaca tttaatttta agcgaaacta aacaaaggcg caaaatgttt ttgccatttt
    26041 tacctctgat tcttgcttca gcaggtttaa ccttattttc cggagagctg gaacgaacag
    26101 aaccatggtt gaatttacca cttgccgtga atctgtctct cattattctt ttcagttttc
    26161 ttctggcgca aatgcctcaa tggctccggc aatttagcaa attgaagcga tttccagaag
    26221 tcagaaaagg aagctattcc tcaacaaaat tctcccgacc aagaaccctg attttgattg
    26281 gctggctggc tcttgtttat ggcgaacatt tagatttacg cataggtcat ttatttaata
    26341 acattactga agctgaatcg gtaagttttg gtgtattact gctgctttac tggcttgctg
    26401 acgctgttgc cgcaattcca gtttatcaat ggaatgctca tgggcttgaa gaaaagatca
    26461 aaaagtctgt tctgcacctg cgcctgcagt taccggtgct ggctctgatt atcatccaga
    26521 cagtctggtt ttggattacc agcaagtttc ttctttcctt cacttcaaac tggagtctta
    26581 tatttgaatt gttgtgcagt ttaatcctga tggtgcttgt tgctccagtt gtttttgtga
    26641 aaagctgggg ggcaaaagct atcgaaaacg ggaatgattt tgaagaaatc cgtaaagaac
    26701 tggaaaattc ccggacaccg gtaacagcaa ttttatcgtg gccggattcg ataatgccat
    26761 actccactgc aggagtaatt ggcttcgtgc gtggattccg ctatttactg atcagcccgc
    26821 aactgctcaa atcactttca gcaacggaac tgcgagctgt aactgctcat gaagccggac
    26881 acttacggaa gcagcatctg cttttttatc tgcttgcatt tatatgttta ctggagttgt
    26941 ttgcatttgc aggcagtgca aacttattgc tgacctggac aggagttttg gaggtttcag
    27001 gaatgctgat gggtgttgcg tctattttaa gcattatatt gtttattcgt tttggtatcg
    27061 gctttctaag ccaaaatttt gaacggcagg cagactgcca cgcttttgaa cgtcacggaa
    27121 tttcacccat ctcaactgcc ctcatgaaag tttccctgct caacggaatc aatccggaac
    27181 aggacaactg gcaccattac ggaattcagc agagaattga ttttttatcg atttgtctaa
    27241 aaaagccgga aatgctacaa aaacatcacc gacgcgtgtt ccggattaag ttggtgtgcg
    27301 cagtattgct tgtaggactt cttggcgcaa attatatgct cagttcagac actttaaaaa
    27361 taaaggtcct tgcctggaaa ttggaacagt cagcagataa ttggcaactg aaagatgctc
    27421 caatgctgac aaaaatgggt gacctgcttt attttcaaga tcagaaaact gaagcagaat
    27481 tatggtaccg ccgtgcgctg gagatgaatc cggaggaatc acacacactg aataacctcg
    27541 cctggctttt aactgaaaag cataacaatg ataagaaacg tctccgggaa agtatagagc
    27601 ttgctcaaaa agcatcaaca ctaaaacagg cggcatttat ctgggacacc ttggcagaag
    27661 catatttaat taacggaaaa tacgaagctg cagctgatgc tgcacgccaa gccctgaagc
    27721 tcgcaaaagc taagat
//
LOCUS       DTVD01000120            8544 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2274_length_8544_cov_489.644245, whole genome
            shotgun sequence.
ACCESSION   DTVD01000120 DTVD01000000
VERSION     DTVD01000120.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2274_length_8544_cov_489.644245"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="EYO68_04255"
     CDS             complement(<1..212)
                     /locus_tag="EYO68_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIC07051.1"
                     /translation="MILDAFTFIVPLVVLIIVLVSMLKILREYERGVVFMLGRFWSVK
                     GPGLIIIIPILQEMVRVDLRTKVLDVP"
     gene            complement(209..1555)
                     /locus_tag="EYO68_04260"
     CDS             complement(209..1555)
                     /locus_tag="EYO68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HIC07052.1"
                     /translation="MLKRFLILIGILGVLWFEVPASTDSSSVILLEVKGPIGPATVDY
                     VERSLEHAKSRKTPLLILQLDTPGGLDASMREIIQQLITSPVPVVTYVAPSGARAASA
                     GTYILYASHIAAMAPGTNLGAATPVQLGASPVPEVPDSSEDNSSVSGSSAMERKIIND
                     AEAYLRSLAQLRGRNVEWAVEAVREGASLSAEEALQQNVIDLIAAHVPDLLAQLDGRE
                     VQLIHEKHVLHTSGLTVKRIEPDWRNELLSLITNPNVAYVLMLIGIYGLIFEFATPGT
                     FFPGIVGAVSLVLSLYAFQVLPISYAGLALMLFGLALMIAEAFAPSFGVLGIGGGLAF
                     IIGSIFLMDTDAPGYGLSPVLVGVLGVANLAFFAFVLGAVFQARKRPVVSGAQTMVGS
                     KAVALEAFEVEGQVRTKGEIWQARSPVPVEAEQALKITGTDGLVLLVKPLNSKEEI"
     gene            complement(1778..2263)
                     /locus_tag="EYO68_04265"
     CDS             complement(1778..2263)
                     /locus_tag="EYO68_04265"
                     /inference="COORDINATES: protein motif:HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIC07053.1"
                     /translation="MEIAEKSTSRVDSAVDVLSGKLKDILAQKEKGVIQVDSDCIVVE
                     AARKMRDNKVGALMVLENGELVGIFTERDLMSRVVAARLDPETVKVSAAMTSSIATVP
                     LETPIREAANLMSQNRIRHLPVLQDGKLCGVISAGDIFAWKLREQEFTLHQLEDYFFK
                     T"
     gene            2525..3163
                     /locus_tag="EYO68_04270"
     CDS             2525..3163
                     /locus_tag="EYO68_04270"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:PRK00081.3-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIC07054.1"
                     /translation="MNSPVTGSLLIGLTGGIASGKSTVLQHLRQAGYSVIDADKLGHK
                     VLEQGNPGYNKVVKCFGNEILNPDGSVNRTALGRIVFVDAEKLKQLNEISHPIIAEMI
                     QKEFEESVSDSNGGIVFLEAALLIEANWYKVCGHIWVVSLDPTVALRRLKERDNLSET
                     EAKLRVGAQLDQEERLAYADVVLQNEGTPEELFTKTQQALRELKQSMNSSHS"
     gene            3197..4174
                     /locus_tag="EYO68_04275"
     CDS             3197..4174
                     /locus_tag="EYO68_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07055.1"
                     /translation="MNHPMHLILDSYSHPGISLFWKRHGLRIMEQLVPNEFFFTTGDT
                     PLEKLVERQIWSGWKKLILIGSPGSIRRGFNTLMQASEECRSTLEIGFWPLDLQELAA
                     SISQSSFHLRPVLQVFKAGHTLLVDVMKVQFLAPELETKYFWNDFVINSTQANAETTI
                     YIDGQNSQVNGKFRCRIAFHDESLNSLTMHPGKLTRTPVLKVYLKRDEGLTTVDRFKQ
                     MKQWFAAEKGNADEETLLKTGKQVEVQGNWANLTLDVSGMQDTVQSVHFEVARKSFSL
                     IVPAKPIQTLESTKKILPAFRPTGAIANNRGSSKRVLLKHKKNSLFYKD"
     gene            complement(4198..4680)
                     /locus_tag="EYO68_04280"
     CDS             complement(4198..4680)
                     /locus_tag="EYO68_04280"
                     /inference="COORDINATES: protein motif:HMM:PF04519.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIC07056.1"
                     /translation="MFGSDKKVPAPGSVPTYVGKGMRIEGKIWGTRPIWIEGEVRGSV
                     DCDSEVIIGESGKVDATIRASIIKVNGFVEGELFASKQIEIMSKGRVRGNITNIPGSL
                     VIHDGGIVEGLCSTETAEKMKNLLPQSFPKLLPEETDSSVKEIETTENKEILESPQTA
                     "
     gene            4988..5482
                     /locus_tag="EYO68_04285"
     CDS             4988..5482
                     /locus_tag="EYO68_04285"
                     /inference="COORDINATES: protein motif:HMM:PF03918.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HIC07057.1"
                     /translation="MQHRQIFAADFRTLCGLFALIILCSTFTVTFAAESEEFEARFRK
                     LSNELRCPTCQGLSVKDSEAGFSNSIKDKIRELMKHKKSDKEIREYFIERYGEWILRA
                     PPKQGFNLVLWILPGAGIVIGLLWVVFRSKSWVTNSQHEELAQLTPEEEQKLKEDLGR
                     FEES"
     gene            complement(5479..6540)
                     /locus_tag="EYO68_04290"
     CDS             complement(5479..6540)
                     /locus_tag="EYO68_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07058.1"
                     /translation="MQNFHPNLIRLLVFDLDSLTVNFDQEKWDNFRQFLLMLDDRDFE
                     TLLISGSIEASEWESFAKLSVLQSTAENAFQNNPALSAKDVFWFSEQPELQKKISRTS
                     QNFAGSDGCTTQNGGLQYQHLHDMLQIFHPSRISAADLSANLFKLKQHSQQVPLMLGI
                     GGPDECGHSFFVGELTDALEDQELLVSIIDLSQVLGTEFQKKSVSTDKSSSSLWRSEE
                     IRDWVVEEVLRPYSRGQQVYFENAPQFIQEYELCTFPFFLAPEMILLIWGTTVFLPEF
                     EKLIDVRVLLELSAKTAAARLFALDDRENFDQSFVETYLQKEGKYYADYLSDYDVPNK
                     IDYRINFENFNAFRLKEKT"
     gene            6621..8024
                     /locus_tag="EYO68_04295"
     CDS             6621..8024
                     /locus_tag="EYO68_04295"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIC07059.1"
                     /translation="MLKRHTQVFASVLRLMDMFLAFGAWELAYQLRFHWINFPPASMI
                     PSHEEYLKAALFVAVLTGFVFAFSGVYRLHKVILPRRELSYLLRGTLSLLLLTLVAAF
                     FYREFSFSRIHTLYFLTCFLIILFFSRLLSRSTLHWLHARGTHVEHVLLIGNGVSANK
                     FIERLESLKSLGIVLKGVIEIGSSSNSDIPQDIPRIGKIEQLNQIIQANQIDQVFIAL
                     APEDQHMLPELKEMLAEQWVDVRIIPDLGTFRTMHTEIESFDDMPIITIVQSPMTGWN
                     QVLKRLLDISGSFFAIVIFAPGMLLISILIKLTSSGPALYGQERMGLDGRTFRALKFR
                     SMQIDAESQTGAVWASENDERRTKFGIFLRRYSLDELPQLFNVFKGEMSLVGPRPERP
                     VFIEQFKSQIPHYMLRHKVKAGITGWAQINGWRGNTSLEKRIECDLYYIERWSIWFDL
                     KILFLTLFRGIFDPNAY"
     gene            8014..8454
                     /locus_tag="EYO68_04300"
     CDS             8014..8454
                     /locus_tag="EYO68_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC07060.1"
                     /translation="MRTDLNTGGFTLYEIMISITIVGIITGLLSVSLFPLLDRSEFVN
                     TADQIKDSLRQAQWLALNRRESHRLKSEFGKLLLQRKNNGSYETVLEEKVPEKISISA
                     TRWPSFSAFGFAAGGTITLETEYYITKVVVSPIGRIRQTEILKK"
BASE COUNT         2534 a         1855 c         1759 g         2396 t
ORIGIN      
        1 ggcacatcga gtaccttggt tcgaagatcg actctaacca tttcctgtaa aatcggaatg
       61 atgataatca gccccggtcc tttgaccgac caaaagcggc ccagcataaa gactacacca
      121 cgctcgtatt cgcgcagaat ttttagcatg ctgaccaaaa caatgatcag tacaactaaa
      181 ggaacaatga atgtaaatgc atctaaaatc atatttcctc cttagaattg agaggtttaa
      241 caagaagcac caaaccgtcg gtgccggtga ttttcaaagc ctgctccgct tcaaccggaa
      301 caggagaacg cgcctgccag atttcacctt tagtccggac ctgcccttca acctcaaatg
      361 cttccagcgc aactgccttg gatccgacca tggtctgcgc tccactgacc accggtcgtt
      421 ttcgggcttg aaagacggct cccagcacaa aagcaaaaaa ggccaaattc gccacgccga
      481 gcactcccac caaaacagga gagagtccgt agcctggcgc atccgtgtcc atcaaaaaaa
      541 tagaaccgat tataaatgcc agaccgccgc caataccaag gactccaaaa ctgggtgcaa
      601 acgcttctgc aatcatcagg gctaaaccaa acaacatcaa ggctaatccg gcataactga
      661 tgggtagaac ctggaaggca taaagggaaa gcacaaggga aacagcaccc acaattccgg
      721 gaaagaaagt tccgggagtg gcaaattcaa agatgagacc gtaaatgcca atcagcatta
      781 aaacataagc cacatttgga tttgtgatca gcgaaagcaa ttcatttctc caatcgggct
      841 cgattcgttt tacagttagt ccggaggtgt gcagaacgtg cttttcatga ataagctgga
      901 cttcgcgtcc atccaactga gcgagcaaat ccggaacatg agctgcaatc aaatcgatca
      961 cgttttgctg tagagcctct tccgcggaaa gactggctcc ttcgcggacg gcctccacgg
     1021 cccactctac attgcgtccg cgcaactgtg ccaaacttct gagataggct tcggcatcat
     1081 tgattatttt gcgctccatt gcggagcttc cggatacgga tgagttgtct tcagatgagt
     1141 ccggaacttc cggaactgga gaagctccca attgaaccgg agtggcggcg cccaaattgg
     1201 ttccgggagc catggcagca atgtggctgg cgtaaagaat gtaggttccg gcactggctg
     1261 ctcgtgctcc gcttggtgcg acgtatgtaa ctaccggaac gggagaagtt atcaactgtt
     1321 gaattatttc ccgcatcgat gcatccaaac cgcctggagt atccaactgc aaaatcagca
     1381 aaggggtttt tcgactcttc gcatgttcca gcgaccgttc cacataatcc actgtagcag
     1441 gaccaatcgg accctttact tccagcagga ttacagaaga agaatcggtg gacgcaggta
     1501 cttcaaacca caatacaccc aatattccta tcagaatcag aaaccgctta agcatgagaa
     1561 tcacagtgaa aacagtgagt tattcctcaa cctgttcagt aattacctgg ttgtgaagca
     1621 ataaaaaatg caaacatgcc gtaatttaaa tcatggagcc tgatgaatcg aaatctgcaa
     1681 acaaaaacaa tcaaaacagc tctgatctaa ataaatcaat tattcttcac tgcaaacagg
     1741 tcttgaagga aaaatgtcag ctatgaaata ctgattatca ggttttaaaa aaataatcct
     1801 ccaactggtg gagagtgaat tcctgttcac gcagtttcca cgcaaaaata tctccagcag
     1861 aaataacgcc gcacaacttc ccatcttgaa gtaccggaag atgccgtata cgattttgcg
     1921 acattaaatt agcagcttcg cggataggtg tttccagcgg cacagtagca attgatgagg
     1981 tcatggctgc tgaaacttta actgtttctg gatcaagtct tgctgcaaca acacgactca
     2041 tcaggtcccg ttcagtaaaa ataccaacca gttctccgtt ttccagaacc attaaagctc
     2101 caactttatt gtcgcgcatt tttctggctg cctcgactac aatgcagtca ctgtctacct
     2161 gaatcacccc tttttccttt tgtgccagaa tatctttcag ttttcctgac agaacatcca
     2221 ctgccgaatc aacacgagaa gtagatttct ccgcaatttc catatttcct cctgggttga
     2281 aaatagttaa tcaacattga ctaaaaagca gctgaaattt gcttccgcca attattcgga
     2341 ctttgttact ccaaacagaa tctgatacaa ttgatcataa cagtcaatac tttttttgtt
     2401 attatcaaaa attcgtgtct tgatctaact ccattatcac attatgctta cagtccaaaa
     2461 tcctgttgct aaatttcttt aaactgattc aagcaatttt cccactttgc tgaaagatac
     2521 aattatgaat tccccggtaa caggatcgct tctaataggc ttgacaggag gcattgcgtc
     2581 tggtaaaagc actgttcttc agcatctccg acaggcaggt tattcagtga tagacgccga
     2641 caagctggga cacaaagtct tggagcaggg taatcccgga tacaataaag tggtgaaatg
     2701 ttttggaaat gaaatattaa atccggacgg tagtgtcaac aggaccgcac tgggaagaat
     2761 agtttttgta gatgcggaaa agcttaaaca gttgaatgaa atttctcatc ctataattgc
     2821 agaaatgatt caaaaagaat ttgaagaatc tgtttcggac agtaacggag gaattgtgtt
     2881 tctggaagca gccttgctga ttgaagcaaa ttggtataag gtttgcgggc atatttgggt
     2941 tgtatctctg gatccgactg ttgcactccg cagattaaaa gaacgtgata acttgagtga
     3001 aactgaagca aaactgcgtg ttggggcaca acttgatcag gaagaacgtt tagcttatgc
     3061 agatgttgtt ttgcagaatg aaggaactcc ggaagaactt ttcactaaga cacagcaagc
     3121 actgcgggaa ttgaaacaat cgatgaacag cagtcattct taaaaaccgg taagcaccaa
     3181 tccgtataaa attattatga atcatccaat gcacctcatt cttgacagct actcccatcc
     3241 gggtatctcc ttgttctgga agcgtcacgg actaagaatt atggaacagc tggtgccaaa
     3301 tgaatttttc tttactaccg gagatactcc acttgaaaag cttgtggaac gtcaaatttg
     3361 gtcgggttgg aaaaaattga ttttgattgg ttctcctggt tctatcagac ggggatttaa
     3421 cacactgatg caggccagtg aagaatgccg cagcactctt gaaataggat tctggcctct
     3481 cgacttacag gaacttgctg ccagcatcag ccagtcatca tttcatttaa gaccggtttt
     3541 gcaggtattt aaagccggac acactcttct agttgatgta atgaaggttc agtttcttgc
     3601 acctgaactg gagacaaaat atttttggaa tgattttgtg ataaacagca ctcaagctaa
     3661 tgctgagact actatttaca ttgacggaca aaactcccaa gtgaacggta agttccgctg
     3721 tcgaattgca ttccacgacg aaagtttgaa ttcactgaca atgcatccgg gaaagttgac
     3781 aaggactcct gtattgaaag tgtacctgaa acgggatgaa gggctaacga ctgtggaccg
     3841 ctttaagcag atgaagcagt ggtttgctgc agaaaaggga aatgccgatg aagagacact
     3901 tcttaaaaca ggaaagcagg tagaggttca ggggaattgg gctaatctta cgctggatgt
     3961 ctcgggaatg caggatacag tacagtcagt gcattttgag gtggcccgta aatctttttc
     4021 gcttattgta ccggcaaaac ccattcagac gcttgaaagc accaaaaaga ttcttccggc
     4081 atttcgtcca accggtgcaa tagccaacaa ccgcggttct tcaaaaagag ttctactgaa
     4141 acataaaaag aactctttat tttacaagga ctgatattta tttgcagtta aggaatttta
     4201 tgcagtctgc ggagattcta gtatttcttt gttttcagtg gtttcaattt ctttgacaga
     4261 agagtctgtt tcttcaggca gcagtttagg aaaagactgt ggtaacaaat ttttcatctt
     4321 ttctgcagtt tcagttgaac acaggccttc gacaattcct ccatcatgaa taaccaaact
     4381 tccaggaata tttgtgatgt ttccgcgaac acgcccttta gacattatct caatctgttt
     4441 tgaagcaaac agttctcctt ctacaaaacc atttactttt ataattgaag ccctgattgt
     4501 agcatctact ttacctgatt caccaattat aacttcactg tcacaatcaa cacttccccg
     4561 gacctctcct tcaatccaaa taggacgtgt accccaaatt ttcccctcaa tccgcatacc
     4621 tttacctaca taagttggta cagagcctgg ggctggtact tttttgtcac tgccaaacaa
     4681 atataactcc ctttaaaatt ttaaaactaa actgcctctg cattttaaaa agctcttaaa
     4741 tccaccacga cctgtattaa acacttttca atacagattc acaagataaa ttttaatatt
     4801 gatgactgta tagcgaattg aaaataatat ctacataaaa aagtgtttaa taataaagta
     4861 ttatacatta attacctgtt ttacattgca tactttttgt tagaaagata gaatatacga
     4921 attgataaat tagacattgg ttctgagtta tcttaaattt tagaaaatta ttgatacact
     4981 cctgttcatg caacacaggc aaatttttgc tgctgacttc agaactctct gcggattgtt
     5041 tgctttaatt attttatgca gcacttttac agtaactttt gctgcagaaa gcgaagaatt
     5101 cgaagcccgc ttcaggaaac tctctaacga attgcgctgt ccaacctgtc agggattgtc
     5161 tgtgaaagat tctgaagcag gattttcgaa cagcatcaaa gataaaatcc gtgaactgat
     5221 gaaacataaa aaatcagata aagaaataag ggaatatttt attgagcgtt acggagagtg
     5281 gattttacgg gcaccaccaa aacaaggttt caatttagtg ctttggatac ttcctggtgc
     5341 aggaattgta attgggctgt tgtgggtcgt gtttcgttca aaaagctggg tgacaaattc
     5401 acagcatgag gaactggcgc aactgactcc tgaagaggaa caaaagctca aagaggattt
     5461 aggacgtttt gaggagagtt aagttttttc ttttagccgg aaggcattaa agttttcgaa
     5521 atttatcctg taatctattt tgttcggcac atcataatct gacaaatagt ctgcgtaata
     5581 tttgccttct ttctgcagat aggtttcaac aaatgactgg tcgaaatttt ctcgatcatc
     5641 cagtgcaaac aaacgggctg cagcagtttt tgcagaaagt tccagtaaaa cgcgtacatc
     5701 gattaatttt tcaaactcag gcagaaagac tgttgtaccc caaatcagta aaatcatttc
     5761 cggagcaaga aaaaacggga atgtgcataa ctcatactcc tgaataaatt gaggagcatt
     5821 ttcgaaataa acttgctgcc cccggctgta gggtcgcaaa acttcttcga caacccagtc
     5881 tcgaatttct tcggaacgcc agagtgagga agatgattta tctgttgata cagatttttt
     5941 ttgaaattct gtccccaaaa cctgggacag atcgatgatt gagactagca gttcctgatc
     6001 ttcaagtgcg tctgtcagtt cgccaacaaa aaatgagtgg ccgcattcgt caggaccacc
     6061 gataccgagc atcaatggta cctgctgtga atgctgcttc agtttgaaaa ggttagcaga
     6121 caaatctgct gcagaaattc tgctgggatg aaatatttgc agcatgtcgt gtaaatgctg
     6181 atattgcagg ccgccgtttt gagtagtgca gccatcgctt ccagcaaagt tttggctggt
     6241 gcgagatatc tttttttgca gttccggctg ttcggaaaac caaaatacgt ctttggctga
     6301 cagtgcagga ttattttgaa atgcgttttc tgctgtactc tgcaaaacag acagtttggc
     6361 aaaagattcc cattcagaag cctcgataga ccctgaaatc agtaaggtct caaaatcacg
     6421 atcatcgagc atcagcaaaa actgacgaaa attatcccat ttctcctgat caaaatttac
     6481 agtcaggctg tctaaatcaa aaactaaaag tcgaattaaa tttggatgaa aattttgcat
     6541 tactacttaa ataatttttt taatattaac cctgatgctc agtcaaaaag tgacttatca
     6601 gtttcttccg aaaaaagcag atgctgaaaa gacatactca ggtttttgct tcagtcctac
     6661 gactgatgga tatgtttctg gcatttggag catgggaact cgcttatcaa ctgcgctttc
     6721 actggatcaa cttccctcct gcctcgatga ttccctctca cgaggagtac ctgaaagcag
     6781 ccctttttgt tgccgtttta acaggcttcg tctttgcttt cagcggagtt taccgtttgc
     6841 acaaagtcat cctcccgcgc cgtgaattat cttatcttct acgtggaaca ttaagcttgt
     6901 tattgctgac tttggttgcc gcatttttct atcgagaatt ttctttttca cggatccaca
     6961 cactctattt tttaacttgt tttctgatta tactgttttt ttcacgacta ctttcaagat
     7021 caaccttaca ttggctgcac gccaggggta cacatgttga acatgttctg ctgattggaa
     7081 acggagtttc tgcaaacaag tttattgaac gtctcgagag cctgaaatca ctgggaatcg
     7141 tcctcaaagg tgttattgaa atcggcagca gttcaaactc agatattcca caggatattc
     7201 ccagaattgg taaaattgag caactgaacc agattattca ggcaaatcaa attgatcaag
     7261 tttttattgc acttgcccct gaagatcagc acatgctccc ggaactgaaa gaaatgctgg
     7321 ccgagcagtg ggttgatgtc cgcattatac ctgaccttgg caccttccgt acaatgcata
     7381 ctgaaattga atcctttgat gacatgccga taataacaat cgtacaaagc cctatgaccg
     7441 gctggaatca ggtccttaag cgactgctgg atatttccgg ttcatttttt gccatagtta
     7501 tttttgctcc gggaatgctc ttgatttcta tactgatcaa actcacatca tcaggacctg
     7561 ctctttatgg tcaggaacgt atgggacttg acggccggac ctttcgtgct ttaaaatttc
     7621 gttccatgca aattgacgct gaatcgcaaa caggtgcagt ttgggcaagt gaaaatgatg
     7681 aacgccgtac aaaatttggt atctttcttc gcaggtacag tttggatgag ttgccgcagc
     7741 ttttcaatgt attcaaggga gaaatgagcc tggtcggccc gagaccggaa cgtcctgtat
     7801 ttattgagca atttaaaagt caaattccgc attacatgct gcgacataaa gtcaaagcag
     7861 gcattaccgg ctgggctcaa atcaacggct ggcgcggaaa tacatccctg gaaaaacgta
     7921 tcgaatgtga tttgtattat atcgaacgct ggtccatctg gttcgatctt aagattctgt
     7981 tcctgactct gttcagagga atttttgatc ccaatgcgta ctgacttaaa cacaggagga
     8041 tttaccttat acgaaattat gatttccatt actatagtgg ggattataac aggcctgctt
     8101 tccgtttctc tctttcccct gctggaccgc tctgagtttg taaacactgc agatcagata
     8161 aaagacagtc tccggcaggc acagtggctg gcactgaaca ggcgggaatc tcaccggttg
     8221 aaaagcgaat tcggcaaact actcctgcag cgaaaaaaca acggcagcta tgaaaccgtt
     8281 ttggaggaaa aagtaccgga aaaaatctca atcagcgcca cacgctggcc ttcattcagt
     8341 gctttcggtt ttgctgcagg cggaactatc acactggaaa ctgaatatta cattacaaaa
     8401 gtcgtggtca gtccgattgg acgaatcagg cagactgaaa tcttgaaaaa ataaaatgtt
     8461 taatcaaaaa agaaacttga agtacccaag tttatttttg atctgctttt ttatatcaac
     8521 tgctttgttt ttgaattctt gcgg
//
LOCUS       DTVD01000121           27674 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_227_length_27674_cov_311.905464, whole genome
            shotgun sequence.
ACCESSION   DTVD01000121 DTVD01000000
VERSION     DTVD01000121.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 27674)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27674)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27674
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227_length_27674_cov_311.905464"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            241..1161
                     /locus_tag="EYO68_04305"
     CDS             241..1161
                     /locus_tag="EYO68_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07061.1"
                     /translation="MNLLNYNHGISSLFILSIRFYQKNIIPILFLTAALIAPAFMIGF
                     AGWGEPESVVFFLSVHLLEGAITLGVIGTAFGSYFPSLGILRALRSPLILGAVHVAIL
                     QYLLFITGVMGLTIPFPFSILVIALWLGGLLLTSMAQQVFIVEGVRGIRAMARSIQLA
                     RTNLSRVFVVVVISTLMQFFVFAILFTLFMPDLNLNIDAEDNDAVPLLLSEILSDPGI
                     HTAIRWAQYLASLLFYPFASLVMTFLYFDLAQRQQVLNMDHLAKFSNQFFGTPLAGTA
                     EPASQAEEEVVEPSEVEILNPDSSEEDKEK"
     gene            1158..1727
                     /locus_tag="EYO68_04310"
     CDS             1158..1727
                     /locus_tag="EYO68_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HIC07062.1"
                     /translation="MKIHSAEFILSAASPWQFPVPSLPEIAFAGRSNVGKSTLINSLL
                     NRKKLVKTSSTPGKTQLINFFKINDEFYFVDLPGYGFAKVPESVSKQWQRLIEAYLQE
                     RETLRNVVLIVDSRHKPTAQDRQLKEWLDYYQRPVLIVASKIDKLKRGQIQKQLKQIK
                     QDLSLDKTPLVHSSLEKGRREEIWNNLWP"
     gene            complement(1956..2387)
                     /locus_tag="EYO68_04315"
     CDS             complement(1956..2387)
                     /locus_tag="EYO68_04315"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIC07063.1"
                     /translation="MPLEQTFSIIKPDGVRRNLVGKILSRFEEKGLRIVATKMIHMSK
                     LEAEGFYAVHRERPFFGELTEFMSSGPVVVSVLEGENAVKYHREIMGATNPADADEGT
                     IRRDFAESLGENTVHGSDSLENAAIEISYFFSQTECVNSVS"
     gene            complement(2486..3088)
                     /gene="hisH"
                     /locus_tag="EYO68_04320"
     CDS             complement(2486..3088)
                     /gene="hisH"
                     /locus_tag="EYO68_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIC07064.1"
                     /translation="MNVIIDYKVGNLYNLKNALDFSGVENQLVSHADELKEADRILLP
                     GVGAFGPAMEHLRNSGMLEALQEKVQAGTPLLGICVGAQLLMDESEEDGSRVGLGWIP
                     GKVKRFQHELKIPQIGWNQVSQQRKDPLFQDVLDEMHFYFVHSYHLRPENSGHVLGLS
                     NYGYDFAAVVRKDNLWGVQFHPEKSQNAGLQLLKNFCTLT"
     gene            complement(3094..3687)
                     /gene="hisB"
                     /locus_tag="EYO68_04325"
     CDS             complement(3094..3687)
                     /gene="hisB"
                     /locus_tag="EYO68_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIC07065.1"
                     /translation="MKQKRVAKIERVTKETKIKLELNLDGSGKSEINTGIGFLDHMLN
                     LWAFHGLFDLKLTCSGDLEIDAHHSTEDIGLALGSALAEAVGEKKGIFRYGHSYVPMD
                     EALIRAALDLSGRPEFVFSGEFTQPSIGQLDTQMINHFFKSLAISSKMTLHLSILYGI
                     NDHHKCEGMFKSMARALRMAVESDPRRTEVVSTKGSL"
     gene            complement(3719..5299)
                     /locus_tag="EYO68_04330"
     CDS             complement(3719..5299)
                     /locus_tag="EYO68_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07066.1"
                     /translation="MPACLRICFVRLFSIVPALVLFVLSAGPGTATAQTHFVPLERGD
                     VSERKEHLTFSPGVELSGDYRLRISKINSTSLPVSRAGTNSAEEFSFDQDLRLRLRST
                     VHRIISINLELATNQEAIYQSDIRTSRSSRTTGAESQTANLTARQSYLELNRNPNEVT
                     KIGKHAINIGDRNGKVFSGILSGYSQRCKAGTWCYEIGGMKLSSADGDWLYFFSLDYP
                     FWHEVDSQGEVIDSFRIEMFRIKYTEHDVPLGMNNIPAKRLSSSTLSSLESGGFTSGS
                     DCNTSLSAYTLNSSCKPIYYNAHEQEYFGLRMQWETPVWGVYADIISNQGNRNYFQYD
                     DRHNMDKRKISGGAAELEISWKKPGEEYMLVGMMAQGDEQLDDSSRNGTNYLRGLESF
                     YEISPGTYRGTKFYFNGGSPDLNSGTGLGHSINNTQMGGFRYRYDIPETTAVYRFGLY
                     ELKRIKPVIDSFGTKSSMIGIEWDNTFTLKMAGHANVDLDFNAFQPARAFSYHDQTVP
                     TGRKDMIFHFAGRLTYSF"
     gene            complement(5422..5913)
                     /locus_tag="EYO68_04335"
     CDS             complement(5422..5913)
                     /locus_tag="EYO68_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07067.1"
                     /translation="MYKLQLDREFSQDLFSESSKEIRDWVVNAIANIVVADDIIEKHE
                     FVALQEAMGLLDSKEEILDLMKKVKERNLFEVKKIKMDPDLALKIFFYLAAIAVIDGS
                     LKKSEAELLKKCGNCLDLEVDFIRAVISWSVKQMEINRKLTQDLKTSNTHRNRIIESI
                     IMS"
     gene            complement(5957..7297)
                     /locus_tag="EYO68_04340"
     CDS             complement(5957..7297)
                     /locus_tag="EYO68_04340"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIC07068.1"
                     /translation="MKNSQVSSAIHKQVKQAREAFLVLANLENLHKNNVLHELAQALR
                     ENSAEILAENARDMAEAQKMLECGELSASACNRVLLNEEKIEQMAQNCESVAALPDPA
                     GKIIQATRLDEGLDLYRVSCPIGVILVIFESRPDVVIQISALALKSGNAVILKGGKEA
                     QNSNRVLAKLIRNTLAKNDTVPENAVSLVESRDEVSQLVKMSSELDLIIPRGSNELVR
                     NIQKNTQVPVLGHADGLCHVYLDQAADEKKSIEVALDAKLDYPAVCNAVETLLVHQKY
                     PVEWLVEILRKMQEAGVELRVCQQTQKRLEKFTELKLKNAVEEDWTTEYIDLIISVKS
                     VKSAEEAVRHINHFGSRHTDTIVTENSELAEWFMNTVDAAGVFWNASTRFADGFRYGF
                     GAEVGVSTGKTHSRGPVGLEGLVIYKYKLYGNGQGAGQYGQGKREFLHEEIQYK"
     gene            complement(7344..9827)
                     /gene="bamA"
                     /locus_tag="EYO68_04345"
     CDS             complement(7344..9827)
                     /gene="bamA"
                     /locus_tag="EYO68_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIC07069.1"
                     /translation="MNFLSSVFRFLFAIVFLGFLCLSNFNQVLYAQTTSSADLSSLIS
                     EIEIKGTSDLESSQILFLIESQVGEPLSRKAVRRDIHSIYQMKLFEDVQAEVKLQDFE
                     ESGAKGYLLRFVVKERPRLSEVKLVGVMLVERTEIEEKMTLVQYDPFDQEKIAVNEQI
                     ILEYYRSEGYSGVRVKSKVEAVESSLEAEGKKFRVVFEMEEKPRVYLSDIYISGTKFY
                     SELDIKRFILSSEIDCVAWANQSGLFREEMINQDLSLITQHYLKNGFIKVFIEKPDVT
                     LIHNPDYSRVDVRLNITEGDQYYSGKISVSGDILGEKEKLMEGLLLEEGEIYNPFLQN
                     RDRSGISEIYHERGYAFVRVVPETKVHKETKTVDVTYRILKGEKAYIGRLEIAGNIET
                     RDHVIRREFELQEEELFNGKKLRLSQENLNRLGFFQSGVILERSPRDQEDNLLDIMAR
                     LKEAQTGTFQAQVGYSDFSGFSGGVTLSKGNLLGSGRTLRLSAQFAEQSVQQKFDITL
                     IDPRLFDSQVSGSIFASRSKVQDSTEFGRGIITENNYGFSLAMPLYFRNLRFGTQISS
                     LDRIFSDSGDDIYKHSVSPSLTYNTVNHPVFPSGGLKTSFRVIQTGTPFGGNIQLREY
                     RLQYQQFWAMNQNNTLILMAKGRFGLLQEQGGSPIPSEDRYRIGGIDSVRGHYYYSIA
                     GPYGPSEQLRNREYRVITDELGYQQTKTYDSRAVGLSTSELQELKSGGISERVFNLEL
                     LFPLSQDENSFVRGLVFLDAGNVNAESRQYQLLGEKEPGFFDLRKSAGFGVRVITPMG
                     VLRFEYGSKLDKRPDETPDRFEFTVSGLF"
     gene            complement(9882..10565)
                     /locus_tag="EYO68_04350"
     CDS             complement(9882..10565)
                     /locus_tag="EYO68_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07070.1"
                     /translation="MQIQVSNLHKSYIDGEGLKLHILRGLNFEAKSGSTVAIVGTSGT
                     GKSTFLHTIGMLEGIDEGQLYLDGSDLANLTRDQAAQFRNEKVGFIFQFHQLLQDFTA
                     LENVMIPQLIQGKNTGIVRKYAFELLEKVGLSERAGHKPTQLSGGEQQRVAIARALVN
                     RPRIVLADEPTGNLDQETGEQVMEILLRLNRENETTLVMITHNTSLASAMQYQHRMEN
                     GQLHLLTND"
     gene            complement(10643..12433)
                     /locus_tag="EYO68_04355"
     CDS             complement(10643..12433)
                     /locus_tag="EYO68_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIC07071.1"
                     /translation="MTASMEKSINKNLIEMLEKSRKLYADRPLYGTKRDGIYEWTTYE
                     QFAEKVDALRGGLASLGVTAGDKVAIICKNTEEWAVSAYATYGLCAQHIPMYETQMAK
                     EWEYIIRDCGAKVLLAANTTVLEQTRDFTERIEELEHIVLLSGSDAGDVKTYSDLLKT
                     GRANPINVKQPESQSPMGMIYTSGTTGDPKGVVLSHRNLIFECDVVLPLLGMTKDDRS
                     LAFLPWAHVFGQVAEVHGLINSGGSAGLVDDVNTLIEELGVVKPSMFFAVPRVYNRIY
                     ERLIGQMQEKPGFIRSLFYGGLKQAKREREGETLSFMENLTLTLARKIIFKKVLNRFG
                     GNLRIAVSGASALSPQVAEFVNDIGISVYEGYGLSENTAALSVNYPGARRFGSVGKPL
                     PGVRIEIDKSVEGSKEEDGEVIAYGENIMLGYHNLPEFTKENITEEGGLRTGDLGHLD
                     EDGYLYITGRVKEQYKLENGKYVAPAPLEESLKLSAYVDQIMLYGFNRPHNVAVIVAA
                     MPAVEKFAEENKISGSGPSLLNEPEIRKLFQEQLEHYGKDFKSFERPQNFALLSEEWG
                     IDNGLLTPTLKLKREVVEEQFKSQIESLYS"
     gene            complement(12593..13903)
                     /locus_tag="EYO68_04360"
     CDS             complement(12593..13903)
                     /locus_tag="EYO68_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIC07072.1"
                     /translation="MKTALTIRNAEVVLPVRTERTNVSVISGRICAIGNEIPDVGEVI
                     DADGLFLLPGIIDPQVHFREPGADHKEDLHSGSCAAAAGGVTSFLDMPNNTPPVITRQ
                     RMQEKKQLAAEKSVVNYGFFMGATPDNLEELNAVENVCGIKIFMGSSTGDLLVDDPDV
                     LEKIFANGSRLIAVHAEDESVLQANKALLKDISDVRIHTLLRNESAALRATKLAVRFS
                     LKYRRRLHILHLTTEDECDFLRTLPREHRISTEVCPQHFTLTAPECYEQLGTLAQMNP
                     PLRTKRHADALWQALKDGIIDCIATDHAPHTLAEKDQPYGKAPAGMPGVETSLALMLD
                     RVNRNVCTLQEVVYWMSESPAKLYAMQGKGRIEVGQDADLVLVDMSLKKTVTNGELQT
                     RVNWSPYHGMELKGWPVQTIVNGQTVFLNGEVDKSVRGREISFA"
     gene            complement(13900..14889)
                     /gene="hemB"
                     /locus_tag="EYO68_04365"
     CDS             complement(13900..14889)
                     /gene="hemB"
                     /locus_tag="EYO68_04365"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIC07073.1"
                     /translation="MDLVYRPRRLRRTAPIRGLVRETELSPRQLIYPLFVTEGKSVRD
                     HIDSMPGQFRLSTDELVRECEELYALGVGAVNLFGYSEEKDELATKSYDSDGLVQRAI
                     RTIKSAVPDLCVQTDVALDPYTTHGHDGLVIDGEIVNDESVEVLCKMALSHAEAGADW
                     IAPSDMMDGRIGAIRNALDVQGFSQVGILAYSAKYASSFYGPFRGALQSAPKSGNKKT
                     YQMDPANIREALKEIALDLEEGADVVMIKPALCYLDVISRVREAFDAPVAAYNVSGEY
                     AMVVAAAEKGWVDRDSAMMEMLLSIRRAGADMILTYFAKDAAALLRADYGSFR"
     gene            complement(15019..15381)
                     /locus_tag="EYO68_04370"
     CDS             complement(15019..15381)
                     /locus_tag="EYO68_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIC07074.1"
                     /translation="MKRDAEFLRKVTELASKNNMGATAKALKIPVYTLKARLLSAAMK
                     LGKIPPQFAKTRVVKKVAKPKKRKHLDTVRASGKAGSSRRVIIPQQIFQELDWNKGDK
                     IVIRRSGKNKIVIEKSAG"
     gene            complement(15537..17219)
                     /locus_tag="EYO68_04375"
     CDS             complement(15537..17219)
                     /locus_tag="EYO68_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07075.1"
                     /translation="MRESVNLTQAFSRILRALLTRSRSQYAALMVFSGMLLADSLFRI
                     YLFPLYNNFLLKIGANPVWSDLDVGFAPIVWLSFFAIILGSLTIVITFASQNVPKLID
                     LYMDHWPSLFFVWWAAACLIHAFTIKILSEAGVEIVSSLIFNYHVLLAVSLVIGFPFV
                     LSILKSTKTSNVIENLLQGSYAKFKRLAKKGPTGIISPEEHAKIQYQLFEELNQLMDL
                     LVFVPYKEPKSQIIEGIGELLRYYVKLKPKFPEDFYKIDQQIHDDISFRTLKSLLAEV
                     EKSHTFYEQKSFRMIGNVYNVFLDKGEFDLSTLCVEQLSQVGKTAIECDDQELIEVIT
                     VRLNTYFRFALKHGQHNNEPRNLYNLVFHYGQFLSYLVEHRQVDRVKTCIGYFVFYGQ
                     QCFNAIPRARSLAFILDILASEMQKLMQIIYQNNWERKLQQELLQKFLLFDNFQDMDK
                     EIAIQFFSQNHGIRLLHIGLALFYLNQNEKEFAETIAKDTLQDLLLLGEPLFSKTMDT
                     IFARLQYSGAKFWEDTDRGNVNIYYTPYQYEIEYFKKLQVKFKLESQKKIPA"
     gene            complement(17277..17516)
                     /locus_tag="EYO68_04380"
     CDS             complement(17277..17516)
                     /locus_tag="EYO68_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIC07076.1"
                     /translation="MKESYLNLMEEHKQDEKVNLFEKVITMTKRAKSLYSLEESARAG
                     LKHKPTFQAILENNEGRLTMKRVQTENLEEDSDQA"
     gene            complement(17695..18369)
                     /locus_tag="EYO68_04385"
     CDS             complement(17695..18369)
                     /locus_tag="EYO68_04385"
                     /inference="COORDINATES: protein motif:HMM:PF09976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07077.1"
                     /translation="MSDAEQMSRKELLQPNRLEKKLFAFVDHAYRKKRLYISAAVAVV
                     VLILAIWGGWKYVQNERINQANQYHIARAMLTNPALSEDERLTQGISALRDFAKSESG
                     TTLSVLALMESGEAYARQTQIDESIAVFQDVIKHPEATTFLRNAARLSLAALFEQQQR
                     WDEAEMMLDSINIASWEDVRWRAMARIAIAKGETEKAKNLLEQLLEKAPDSVFRQETE
                     ILLLSL"
     gene            complement(18724..20337)
                     /locus_tag="EYO68_04390"
     CDS             complement(18724..20337)
                     /locus_tag="EYO68_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC07078.1"
                     /translation="MSDSKNMILDFVAEGFQQGWKHIDASRLAADQSLEADVVIIGSG
                     AGGGNSAEILAQSGLNVIIVEEGPLQTSRDFHMKENEAYSNLYQESAGRQTKDRGIKI
                     FQGRMVGGGTGINWTTSFRTPAETLKYWAEEMGVLGFSEDEMLPWFEWSEKRYNIQDW
                     KVPPNANNSALETGCEKLGVSWGKIRRNVTGCLNLGYCGTGCPVNAKQSTLVTTIPGA
                     LKEGATLISKARAETIEIKNGKITELKCKAMTPRGNAPAVQTIKIKARHYVLAAGSIG
                     SPAIMLRSENKILNPYGLVGTRTFLHPVNISGAIMPFPVNGEYGAPQTSYSDHYIETR
                     LDNQKSGFKLECPPLQPMLVATALEGHGKVHAEIMRQRPFLQVLVALQRDGFHPESIG
                     GKVYLKSDGTPGLDYPVSGYVWGGIRDAFIAMSEIQFASGAKAVAPVHRDAGLYHSLD
                     KVKKAIASLPMETLKARLFSAHVMGGCPMGENAAKSVVNSKGRHHEVENLSIFDGSVF
                     PTSIAANPMESIFAVTAKFATELAKVLVM"
     gene            complement(20568..21125)
                     /locus_tag="EYO68_04395"
     CDS             complement(20568..21125)
                     /locus_tag="EYO68_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07079.1"
                     /translation="MLTRRNLFKLGAVSGVTLLAIETFHASLEIPPALDGLGHSFEFL
                     TPVDRGILSKFTPVILAEALPESNEERVLAIKEVIVGWDIAVSGLPPLTQQEIRKLFL
                     VMDSNFIFSLTPRLLTGVPGAWDDPSEIDDCLNNWRLSDPESLITGNELRVGYMGMVE
                     LTMASWYANPRSWDFCSYAGPPVLF"
     gene            complement(21198..22043)
                     /gene="mazG"
                     /locus_tag="EYO68_04400"
     CDS             complement(21198..22043)
                     /gene="mazG"
                     /locus_tag="EYO68_04400"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIC07080.1"
                     /translation="MDEKKTPALTPIENLKTVMARLRHPEEGCPWDREQTFASVAPYT
                     IEEAYEVSEAIDSKDMSSLKEELGDLLLQIVFHSRMAEESGDFNFDDVAQTISDKMIC
                     RHPHVFGKESERSKEFHSGEWEAQKRRERVEKAQRHGNAASVSVLDDVALALPALMRA
                     EKLGKRVAKVGFEWPEIDSVFEKINEEIQELRSELEANEISEESIEEELGDILLTVAT
                     LARHVHVDPELALRRANAKFERRFRFVEESLAEDGQSPENVSLQKLEKLWQEAKQRKL
                     TKGET"
     gene            complement(22045..22827)
                     /locus_tag="EYO68_04405"
     CDS             complement(22045..22827)
                     /locus_tag="EYO68_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIC07081.1"
                     /translation="MSSDDAKIGIVGGAGPYAGLDLAQKILQQTSAIIDQDYLPTISI
                     STPADIADRTRFLLGQTTKNPAHAIFRNLTELEDLGATVAGFPCNTAHAPAIRDVFME
                     KLKQSGSRLKLLDMIAETVDFLRETCPEVKIVGVLSTIGTWKAGFYPKLLGEAGYEVR
                     ILEEPQQQRLHDEALFHPEYGIKVQAHPVSKAARKVLLEGVQELEKQDVQAVVLGCTE
                     IPLGLPERKLGSTYLIDPGLILARALIREFCPEKLLPWSWKT"
     gene            complement(22879..23670)
                     /locus_tag="EYO68_04410"
     CDS             complement(22879..23670)
                     /locus_tag="EYO68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07082.1"
                     /translation="MLSVNNIEVIYDHVILVLKGVSLDVPKGSVVAILGANGAGKTTT
                     LKAISNLLQAERGDVTKGSIDFDGTQVHDLSPNELVKRGCIQVMEGRHVFEHLTVEEN
                     LLTGAYTRKEGRSTIAADLEKTYSYFPRLKERRTSQAGYTSGGEQQMLTIGRALMSKP
                     KMILLDEPSMGLAPQLVEEIFEIVKDLNQREGVSFLLAEQNTNIALRYADYGYVLENG
                     RVVMDGEGAELRENEDVKEFYLGMGGAGRKSFRDGKNYRRRKRWL"
     gene            complement(23761..25074)
                     /locus_tag="EYO68_04415"
     CDS             complement(23761..25074)
                     /locus_tag="EYO68_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07083.1"
                     /translation="MKKKFFLTLVASVITCLWLSQEIMAAKGIYIPLFTYRTGPFAGS
                     GIPNANGMSDYLNMLNERDGGIGGVPIIVEECETGYNTKKGVECYERIKQKNPVVINP
                     YSTGITLQLIPKSPVDKIPVHSMGYGLSAAADGSVFPWVFNYPSTYWNQASAIIKYIG
                     YQEGGMSNLRGKKIGFIYLESGYGREPIPLFEQLSSELGFILFKIAVAGKEMQNQSSH
                     WLKVRKEKPDWMIMWGWGAMNPTAIKEATRIKFNMEKFVGVWWSGGEDDARPAGKAAK
                     GYKSANFHGIGTNYPAVQDILKYVVDKGKSQVKDRSRVGENFYMRGVFNSVLVAEAIR
                     TAQGKFGNRVISSEEMRWGMENLNLTSARLAEIGLPGFANPIKVSCDDHANNGPIFIQ
                     QWNGTSWERVSGWINSMDRVRPMLEKAAADYKAKNSVPDRTIPCS"
     gene            complement(25221..26297)
                     /locus_tag="EYO68_04420"
     CDS             complement(25221..26297)
                     /locus_tag="EYO68_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07084.1"
                     /translation="MLYREAGQFKTTYIRDQELFPVKQDRWGIFVIIGFSFIIVPLIA
                     NDYWLQAILIPFLIYSLSALGLNLLTGYAGQLSLGTGGFMAVGAYSCYKLVTVFPETN
                     VIVLILISGFFSAGIGLIFGIPSLRIKGFYLAVATLAAQFFFEWLFGRVQWFYNYNNS
                     GAIEVPVRNFFDIAVTGPTATTQARYMVVLCIVTFLALVAVNLVRGRIGRAWMAIRDM
                     DIAAELIGIRPFPTKLIAFAISSYYAGVAGALFVFTYLGSAEVEVFDINMSFEILFMV
                     IIGGLGSISGAFLGAAFIVLIPILITNLPLILGIPINSATVEHFNFMILGALIVLFLI
                     FEPHGLAKIWSIVREKLRAWPFPY"
     gene            complement(26315..27259)
                     /locus_tag="EYO68_04425"
     CDS             complement(26315..27259)
                     /locus_tag="EYO68_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07085.1"
                     /translation="MLTTIFIHPFFDMAADPILFLQVIWEGFVTGILYSLIALGFVLI
                     FKASGVFNFAQGIMVVFAALTLVGLHENGIPAYIALPITILVMYALAFSVERLVLRPL
                     VNQPDIILFMATIGVTFFLIGFGEFIFGGEPKMMITEELLLPYGSITFFDDMLILQEI
                     DIAASGIAALALIFLALFLGKTKIGIALRAVADDNQAALSVGVSLNTIWVLVWFISGI
                     IALITGIMWGAKSDVSFALSIIAFKALPVLILGGFTSVQGAIVGGLIIGIGEKIGEFY
                     WGDLVGGSIESWLAYMIALVFLLFRPQGLFGEKIIERV"
     gene            complement(27283..>27674)
                     /locus_tag="EYO68_04430"
     CDS             complement(27283..>27674)
                     /locus_tag="EYO68_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07086.1"
                     /translation="NREYVEDIIDFLEIQAIRRTPVGRLPYGLQKRVELGRALAMEPD
                     LLLLDEPMAGMNIEEKQDMCRFVLDVNNQYGTTIALIEHDMSVVMDISDKVVVLDYGE
                     KLAEGTPDEIRGNQTVIDAYLGVTTAN"
BASE COUNT         7875 a         6406 c         5131 g         8262 t
ORIGIN      
        1 cacgtgattt ccaatcacgc accttcggcc actcggtcac ctctccgctg cagatttgcc
       61 ctgaaaaatc caagaccttt ccagcacata aattttacct gaggtgaccg gtccgtgcaa
      121 gctttttgca aagctttaac caccatcttt aagattctat tctgcacttt tacttgttat
      181 actgtaagag gctttgtatg ctttaggcta aacgttttcc gcaaattttc aacagacatt
      241 atgaatttac taaattacaa ccacggtatt tcatccttat tcattctgag tataagattc
      301 tatcaaaaaa acattattcc aattttgttt ttaactgcgg cattgattgc acctgctttc
      361 atgattggtt ttgcaggttg gggtgaaccg gagagtgtag ttttttttct ttctgtgcat
      421 ttattggagg gggcaattac attaggtgta attggaaccg cattcggcag ttatttcccg
      481 tcactaggca tattacgggc acttcgttca cctttaattc taggggcagt ccatgttgca
      541 atcctgcagt atcttctctt catcacagga gtaatgggcc tgacgatacc ctttcctttc
      601 agtattttgg taattgccct ttggctggga ggattattgc tgacttcaat ggcacaacag
      661 gtatttatcg tagaaggtgt tcgtgggata cgggcaatgg ccaggagcat acagctggcc
      721 cgtacaaatt tgtcacgagt ttttgtggtt gttgtgatca gcactcttat gcaatttttt
      781 gttttcgcca ttctcttcac cttgttcatg cctgacttga atttaaacat tgatgcggaa
      841 gacaatgacg cagtaccttt attactctca gaaattttga gtgatccggg gattcacacg
      901 gcaatccgct gggcacaata tttggcatcc ctgctgttct atccgtttgc atcattggtg
      961 atgaccttcc tctattttga tctggctcaa cgtcagcaag tactgaatat ggatcatctc
     1021 gccaaattct caaaccaatt cttcgggact ccgcttgcag gaacagcaga acctgcaagt
     1081 caagcagaag aggaagtagt tgaaccctct gaagtcgaaa tccttaatcc ggattcgtct
     1141 gaggaggata aagagaaatg aaaatccatt ctgctgaatt tatccttagt gctgcctctc
     1201 catggcaatt ccccgtccct tcacttcctg aaattgcctt tgccggtcgt tcaaacgtgg
     1261 gaaaatccac gctgataaac tctctgctca accgcaaaaa actggttaaa accagctcta
     1321 ctccgggaaa aacgcagttg attaattttt ttaagatcaa tgatgaattt tattttgttg
     1381 acctacccgg atatggtttt gcaaaggttc ctgaaagtgt cagcaaacaa tggcagcgcc
     1441 tgattgaagc ttatttgcag gagcgtgaaa ctctgcggaa tgtagtgctg attgtagaca
     1501 gcagacacaa gccgactgca caggatcggc agctcaagga gtggttggac tattatcagc
     1561 gtccggtcct gattgtggcc agtaaaattg acaaattaaa gcgcggccag attcaaaaac
     1621 agttgaagca aattaagcag gatctctctc tcgacaaaac accgttggtg cactcctctt
     1681 tagaaaaagg gagacgcgaa gaaatatgga ataatttgtg gccttaaaga cgtagttata
     1741 attttgctga ggcccgaatg gaaaccgacc atatcgtttt tgcctgcaat agtatcagaa
     1801 ctcgagagat attaatcctc atataaaact gatattaccg ggtcatctcg actgaagtga
     1861 gaaatctgat aaagcgctga tatcttaaaa gacccctcac aaacgttcgg gatgacacga
     1921 tatatcacgt gattgtcgag ttcctgaaat gaatgtcagg aaactgaatt tacacactca
     1981 gtctgtgaga aaaaataaga aatttcgatg gccgcatttt ccagactgtc tgaaccatgg
     2041 acagtatttt caccaagact ttccgcaaaa tcacgtctga ttgttccctc atccgcatca
     2101 gctggattag tcgctcccat tatttcacga tgatatttaa ccgcgttttc accttccaga
     2161 actgaaacta ctaccggtcc ggaactcata aattccgtca gttccccaaa gaatggccgt
     2221 tcccgatgaa ctgcataaaa tccttctgct tcaagttttg acatatgaat cattttggtg
     2281 gcaacaatac gaaggccttt ttcttcaaag cggctgagta tttttccaac aaggttgcgg
     2341 cgcacaccgt ctggtttgat gattgaaaat gtttgttcta aaggcatttt ttctttttta
     2401 tcaaggttta atattgttcg tgataattct tcagactaaa ttctgccgtt gattaacact
     2461 gataattagt gtgtaaaaca caccgctaag tgagtgtaca gaagtttttc aacagttgca
     2521 gtcccgcatt ttgactcttt tccggatgaa actgaactcc ccacaagttg tctttacgaa
     2581 caactgcggc aaagtcataa ccgtaatttg agagacccaa aacgtgccca ctgttttccg
     2641 gacgcaaatg ataagaatga acaaaataaa agtgcatctc atctaaaaca tcttgaaaaa
     2701 gcggatcttt tctctgctga gaaacctgat tccagccaat ctggggaatt ttcagttcat
     2761 gctggaaacg cttaacttta cctggaatcc agcccaaacc cacacgagaa ccatcttctt
     2821 cgctttcgtc catcagcaat tgtgctccta cgcaaattcc cagcaaagga gttcctgcct
     2881 gaactttttc ctgcagagct tccagcattc ctgagtttcg caaatgttcc atagccggac
     2941 caaatgctcc aactcccgga agcagaattc tgtctgcttc ctttagctca tctgcatgag
     3001 aaacgagctg attttccaca ccagagaaat ccagtgcgtt tttcagattg tacagatttc
     3061 caactttgta atcgatgatt acgttcatgc ctgtcaaaga cttccttttg tagaaaccac
     3121 ttctgtacgt cgtggatctg actcaacagc catacgtagg gcacgcgcca ttgacttgaa
     3181 catgccttcg catttgtgat ggtcgttgat tccgtataat atcgacaggt gcagagtcat
     3241 tttagacgag atagcaagtg atttgaaaaa gtggttgatc atctgtgtat ccaactgacc
     3301 aatgctcggc tgagtaaatt cacctgaaaa gacaaattcc ggacgtccgg acaaatccaa
     3361 agcagcccgg attaacgctt cgtccatggg cacatacgaa tgaccgtagc ggaaaatccc
     3421 tttcttttca ccaacggctt ctgccaacgc actaccaaga gcaagcccga tatcttctgt
     3481 agagtgatgc gcatcaattt ccaaatcacc actgcaggtt aatttcaaat caaacaaacc
     3541 gtgaaaagcc cataaattca gcatgtgatc tagaaaacca attccggtat taatttcaga
     3601 tttaccggaa ccgtcaagat tcagttccag tttaattttt gtttctttgg ttacacgctc
     3661 aattttcgcg actctttttt gtttcatagg tcctcataag tttgttaaaa gcgacagttc
     3721 agaaagagta agtcaaacgt ccggcaaagt gaaaaatcat atctttcctt ccggtaggaa
     3781 ctgtttgatc atgataactg aatgctcttg ccggctgaaa tgcattaaaa tccaaatcta
     3841 cgtttgcgtg accagccatt ttcagtgtaa atgtattgtc ccactcaatg ccgatcatac
     3901 tggatttagt cccaaacgaa tcaataactg gcttgatgcg ctttagttcg taaagaccaa
     3961 atcgataaac agcagttgtt tcaggaatgt catagcgata acgaaaacca cccatttgag
     4021 tattattaat tgagtgcccc aagccggtac cactgttcag gtcggggctg cctccgttga
     4081 aataaaattt ggttccacgg tacgttcctg gtgatatttc gtaaaaactt tccagaccgc
     4141 gaaggtaatt tgtaccattt ctgctgctgt catcaagttg ttcatcaccc tgggccatca
     4201 ttccgacaag catatattct tcacccggtt ttttccaact gatttccagt tcagctgctc
     4261 cgcctgatat ttttctttta tccatgttat gcctgtcatc atactggaaa taattccgat
     4321 tgccctgatt tgaaataata tccgcataaa caccccaaac aggtgtttcc cactgcatac
     4381 gcagtccaaa atattcctgt tcatgtgcat tgtaataaat tggcttacag cttgagttga
     4441 gagtataagc cgacaggctc gtattacagt cacttcctga ggtaaaccct ccactttcca
     4501 aactggaaag agtactgctg gagagacgtt ttgcaggaat attattcatt cccagtggaa
     4561 catcatgttc tgtgtatttg atacggaaca tttcaattcg aaaagaatcg ataacttcgc
     4621 cctgtgaatc tacctcgtgc caaaaaggat agtcgagtga aaagaagtac agccagtctc
     4681 catctgcaga agaaagcttc attccgccaa tttcataaca ccaggttcca gccttgcagc
     4741 gttgcgaata accactgagg attccggaaa ataccttgcc gttacggtct cctatgttaa
     4801 tcgcatgttt accgattttt gttacttcat tcggatttct gttgagttcc agatacgatt
     4861 gccgtgcagt tagatttgca gtttgggact ctgctccagt agtacgtgaa cttcgtgaag
     4921 tcctgatgtc agactggtat atcgcttcct gattagtagc aagttccagg tttattgaaa
     4981 ttatacgatg cacagttgag cgtagacgca aacgtaaatc ctgatcaaaa gaaaactctt
     5041 ctgcagaatt ggttcctgct cgtgaaacag gaagagatgt tgaattaatc tttgaaattc
     5101 gcagtctgta atctccggaa agttcaactc caggggaaaa tgtcaggtgt tctttacgct
     5161 cagaaacgtc acctcgctca aggggtacaa aatgagtttg tgctgttgct gtacctggac
     5221 ctgctgacaa aacaaataaa actagtgcag gaacaatcga aaacaaacgg acaaagcata
     5281 tccgcaaaca ggctggcata agactctgaa aagaaagagg atagatgact tgtataattt
     5341 ttataagagg ataatttata cttgaatatt agtagattaa gttaagaata gcaagttttt
     5401 ttcagcacaa cgtttgtata ttcaactcat tattatactt tcaataatac ggtttcgatg
     5461 tgtgttgctg gtttttaagt cttgagttaa ctttcgattt atttccattt gttttactga
     5521 ccagctaatg acagcacgaa taaaatcaac ttcaagatcc aaacaatttc cgcatttttt
     5581 taataattct gcttcacttt ttttaagact accgtctata actgcaattg cagcaagata
     5641 aaagaaaatt tttagagcaa gatccgggtc cattttgatt ttttttacct cgaatagatt
     5701 tctctctttc acttttttca ttaaatcaag aatttcttcc ttgctgtcca gcaggcccat
     5761 tgcttcctgc aaagccacaa actcatgttt ctcaatgatg tcatctgcaa caactatgtt
     5821 tgcaattgca ttgaccaccc agtccctgat ttcttttgat gactcagaaa aaagatcttg
     5881 tgaaaattca cgatctaatt gcagtttata catgaaagct cctatttcaa tctagtaaaa
     5941 actttgaaat ctacagttat ttatattgaa tttcctcatg tagaaattcc cgcttaccct
     6001 gcccatattg accagcgccc tgcccgtttc catataattt atatttataa attaccaaac
     6061 cttcaagtcc aacaggacct cgtgagtgag ttttaccggt acttactcca acttctgccc
     6121 caaatccgta acgaaatcca tccgcaaatc gagttgacgc attccaaaat acacctgctg
     6181 catccacggt attcatgaac cattctgcca actctgaatt ttcagtaaca attgtatcag
     6241 tatgcctgga accaaaatga ttgatgtgcc taactgcctc ctctgcagat ttgactgatt
     6301 taacagaaat tatcaaatcg atatattcag ttgtccagtc ttcttcaact gcatttttta
     6361 actttaattc agtaaatttt tccagacgtt tttgagtttg ctgacatacc ctcaattcta
     6421 caccggcttc ctgcattttt ctcaaaattt ctaccagcca ctcaaccgga tatttttgat
     6481 gcacaagtaa agtttccaca gcattgcaga cagcgggata atccaacttt gcatccagtg
     6541 caacctcaat gcttttcttt tcatctgcag cttgatccag atacacatga caaagtccat
     6601 ctgcatgtcc cagcactgga acctgcgtat ttttttgaat attccggacc agttcattgc
     6661 tgccgcgggg aatgattaaa tcaagttcag aactcatttt caccaactgt gaaacttcat
     6721 cgcggctttc caccagactg actgcatttt ctggtacagt atcattcttc gcaagagtat
     6781 tccggatcag ttttgccaga acacgatttg aattttgtgc ctctttgcct ccttttaaaa
     6841 ttaccgcatt tccggatttt agagctaatg cagaaatttg tatgaccaca tccggacgtg
     6901 attcaaaaat taccagaatg actccaattg gacaggacac acgatagaga tcaagacctt
     6961 catccagccg ggttgcctga attattttgc ctgcaggatc tggcaatgca gcaacacttt
     7021 cgcagttttg tgccatctgc tcaatctttt cttcattgag taaaacacgg ttgcaggcac
     7081 ttgcagacaa ctcaccacac tccagcattt tttgggcttc agccatatcc ctggcgttct
     7141 ctgccaaaat ttcagccgaa ttttcacgca atgcttgagc taattcatga agaacattgt
     7201 ttttatgcaa attttccaga ttcgccaaaa caaggaaggc ttcacgtgcc tgttttactt
     7261 gcttgtgtat tgctgaggaa acttgagagt ttttcattaa attataagat tgcaatttcg
     7321 gccggacagt tcagcagcat ttgttaaaac aagccgctga cagtgaattc aaaacgatcc
     7381 ggagtttcat cgggacgctt atccagtttc gatccatact caaaacgcag cacacccatt
     7441 ggagttatca cacgaacacc aaagcccgca cttttacgca gatcaaaaaa accgggttct
     7501 ttttcgccaa gtagttgata ttgcctcgat tccgcattaa catttcctgc atccaaaaat
     7561 accagtccgc ggacaaaact attctcatct tgactgagtg gaaacagtaa ttcaagatta
     7621 aagactctct ctgaaattcc gccgctcttt aattcctgca attcgctggt actcaagccg
     7681 acagctctgc tgtcataagt ttttgtctgc tggtaaccca gttcgtcagt gatcactctg
     7741 tattcacgat tacgaagctg ctcgctagga ccgtagggtc cggctatgct gtaataatag
     7801 tgtccccgta ctgaatcaat acctcctata cgatacctgt cttcggatgg aatcggcgaa
     7861 ccaccctgtt cctgcagcaa cccgaaacgt cctttggcca tcaaaattaa agtgttattc
     7921 tggttcattg cccaaaattg ctgatactga agtcggtact cccgcaactg aatattcccg
     7981 ccgaaaggtg tgccggtctg tatcacacga aaggaagttt ttaagcctcc gcttggaaag
     8041 acaggatgat tgacagtgtt gtaggtcaaa gacggtgaaa cagaatgctt ataaatgtca
     8101 tctccggaat cagaaaagat gcgatcaagt gaactgattt gagttccgaa acgtaaattc
     8161 cggaaataaa gtggcattgc caaactaaaa ccatagttgt tttcggtgat tattccacgt
     8221 ccaaattctg ttgaatcctg aaccttgctt cttgaagcaa aaatagaacc tgaaacctgt
     8281 gaatcaaaga gtcttggatc aatcagtgtt atatcaaatt tttgttgaac actctgttct
     8341 gcaaactggg cactaagccg cagagtccgc cctgacccga gcaggttgcc ctttgaaagt
     8401 gtcactcctc cactgaatcc ggaaaaatcg ctatacccta cttgagcctg aaaagttccg
     8461 gtttgggctt ctttaagacg agccatgata tccagcagat tgtcctcttg atcacgtgga
     8521 gatcgttcca ggatcactcc ggactgaaaa aagcctaaac ggttaagatt ttcctgactg
     8581 aggcgcagtt ttttaccatt aaaaagctct tcttcctgta attcaaactc acgccgaatc
     8641 acatgatctc gtgtttcaat atttcccgca atttccagac ggccaatgta ggctttctca
     8701 cccttcaaaa tgcggtaagt tacatcaaca gtttttgttt ctttatgaac ttttgtttcc
     8761 ggtacaaccc tgacaaaagc ataaccccgt tcatggtaaa tttcacttat acctgaacga
     8821 tctctatttt gcagaaacgg attataaatt tcaccttctt ccagcaacaa gccttccatt
     8881 aacttttctt tttcacccag aatgtcaccg gatacagaga tctttccaga gtaatactga
     8941 tctccttctg taatatttaa acggacatcg acccgactat aatcaggatt gtgaatcaac
     9001 gttacatccg gtttttcaat aaatactttt atgaatccat ttttaagata gtgctgagta
     9061 atcagtgaca ggtcctgatt tatcatttct tcacggaaaa ggcctgattg atttgcccag
     9121 gccacacaat caatttcaga actcaggatg aatcttttga tatcaagttc tgaataaaat
     9181 ttagtaccag aaatgtaaat atctgagaga taaacacgag gtttttcctc catctcaaat
     9241 actacacgaa acttttttcc ttcggcttca agactggatt ccacagcttc aactttactt
     9301 ttcacacgaa ctccggaata accctcactt cggtagtact ccaaaattat ttgctcattt
     9361 accgcaattt tttcttgatc aaatggatca tactggacca gggtcatctt ttcttcaatt
     9421 tctgtccgtt caaccagcat tactccaaca agtttcacct ccgacagacg aggacgttcc
     9481 tttaccacaa agcgcagtaa atatcctttt gcgcctgatt cttcaaaatc ctgaagtttt
     9541 acttctgcct gtacatcttc aaacaatttc atttgataaa tggagtggat gtccctgcga
     9601 acagccttac gtgacagagg ttcgccaact tgactttcta tcaaaaacag gatctgagag
     9661 gattccaagt cggatgtgcc ttttatttca atctcagaaa taagtgacga taaatccgca
     9721 ctacttgtcg tttgtgcata gagaacttga ttgaaattgc ttaggcacag gaagcccaga
     9781 aaaacaatcg caaataaaaa acgaaaaaca gaagaaagga agttcatcag gtttgaaaga
     9841 agcaggaatc aggcagagta gaaatccttt tgcagaaatt cttaatcatt cgtaagcaaa
     9901 tgcagttgtc cattttccat acgatgctga tactgcatgg ccgatgcaag gctggtgttg
     9961 tgggtaatca taacaagtgt agtttcattt tctcggttta gtcgcagcaa aatttccatt
    10021 acctgctcac ctgtctcttg gtccaggttt ccggttggtt catctgccag tacaattcgg
    10081 ggtcgattga ctaaagcacg agcaatcgcg acccgctgct gttctcctcc ggaaagctgt
    10141 gtcggcttat gtccagcacg ctcagacagt ccaacttttt ccagcagttc aaaagcatat
    10201 ttccggacaa ttccagtatt tttaccttga atcaactggg gaatcatcac attttccaac
    10261 gcagtaaaat cctgtagtaa ctggtggaat tgaaagataa aaccaacttt ttcattccgg
    10321 aactgtgcag cctggtcgcg ggttaagttg gccaaatcgc tgccgtcaag gtacaactgc
    10381 ccttcgtcta taccttccag cattccaatt gtatgcagaa aagtgctctt tccggttccg
    10441 gaagttccaa ctattgctac agtcgaaccc gactttgctt caaaattaag gccccgcaaa
    10501 atgtgcagct tcaagccttc tccgtcaatg tagcttttgt gcagattaga aacctggatt
    10561 tgcattgtac tgaagaaaca ctaaaatata acagaataac agaaaattac ggcgaatcac
    10621 aagaccaaac gaaccacaaa tattaagaat aaaggctctc aatctgtgac ttgaactgtt
    10681 cctccaccac ctcccgtttc agtttaagag ttggagtcag tagtccattg tcaatacccc
    10741 actcttcaga gagcagggca aaattttgcg gacgttcaaa actcttgaaa tcttttccat
    10801 aatgttccag ttgttcctgg aacaattttc taatttcagg ttcatttaac aaacttggac
    10861 cacttccgga aatcttattt tcctcagcaa atttttccac tgcgggcatc gctgctacaa
    10921 tcacagccac attatgaggt cggttgaagc cgtaaagcat tatttgatca acgtacgcac
    10981 tcagcttcag tgattcttct aggggtgcag gtgccacata cttgccattt tcaagtttgt
    11041 actgctcctt cacacgtcca gtgatataga ggtaaccgtc ctcatccaaa tgccccaggt
    11101 ctcccgtccg caaccctcct tcttcagtga tattttcttt cgtgaattcc ggcaggttgt
    11161 ggtagccaag catgatattt tcaccatatg ctattacctc tccgtcttct tctttggaac
    11221 cttcaacaga tttatctatt tcaatccgta ctccaggaag tggtttaccc accgaaccaa
    11281 agcgtcttgc acccggataa ttaactgaaa gtgctgccgt attttcactt agcccataac
    11341 cctcataaac gctgatgcca atatcattta caaactctgc gacttgtgga ctcaaggcgc
    11401 tggctccact tacagcgata cgcagatttc caccaaagcg gttcaaaact tttttgaaaa
    11461 taattttacg agccagcgtt agtgttaaat tttccataaa actaagagtt tcaccttctc
    11521 tttctctctt tgcctgtttg agtcctccat aaaacaggga gcggataaag cctggttttt
    11581 cctgcatttg acctattaga cgctcataaa tcctgttata aacacgaggc acagcaaaga
    11641 acatgctggg tttgaccacg cccagttctt caatcagcgt attcacatca tcaaccagtc
    11701 ccgcagaacc gccggaattg attaaaccat gtacttctgc cacctggcca aaaacgtgag
    11761 cccaaggcaa aaatgccagt gatctatcat ctttcgtcat gcccagcaaa ggtaagacta
    11821 catcacactc aaaaatcagg ttcctgtgac tcagcaccac accttttgga tcacctgtag
    11881 ttcctgaagt atagatcatt cccatcggtg actgtgattc aggctgcttg acgtttatcg
    11941 ggtttgctct gcctgttttc agtaaatcag aatatgtttt aacatctcct gcatcacttc
    12001 ctgaaagtaa aacaatatgt tccagttctt caatccgttc cgtaaaatca cgagtttgtt
    12061 caagcacagt tgtgtttgcc gccagtaata cttttgcacc acagtcacgg ataatgtatt
    12121 cccattcctt tgccatctgt gtttcataca ttgggatgtg ctgtgcacat aatccataag
    12181 tggcataagc agagactgcc cactcttctg tgtttttaca gataatagcg actttgtctc
    12241 cagcagttac acctaatgaa gccagccctc cgcgtaaagc atctactttt tctgcaaact
    12301 gttcgtaagt agtccactcg taaattccat cgcgtttcgt tccatacaaa ggacgatctg
    12361 cgtaaagctt tcgagatttt tcgagcattt cgattaaatt tttattaata cttttttcca
    12421 ttgatgcagt catcattacc tccaaattta ggttttttta cattgggaga aatcgtaaaa
    12481 tttcaaaata ccacacttgt tattcaaatt tgcttcacag aatcttttcg ttattttttc
    12541 aggtaaatgt taaaataaaa ttacttcaac tatcctaact cttatccact gatcatgcaa
    12601 agctgatttc tcttccacgt acacttttat cgacttctcc attcaaaaaa acagtttgac
    12661 cattaacaat cgtttgaacc ggccagccct ttagttccat accgtgataa ggagaccagt
    12721 taactctggt ctgtaattca ccattcgtaa ccgttttctt taatgacatg tcgactaaaa
    12781 ccaaatctgc atcctggcca acttcgatac gtccttttcc ctgcattgca tataattttg
    12841 caggagattc agacatccag taaaccactt cctgcaaagt gcaaacattg cggttgactc
    12901 tatccagcat taaggccaat gaggtttcga caccgggcat tcctgcaggc gccttaccat
    12961 atggctgatc tttttcggct aaagtgtgag gcgcatggtc tgtggcaatg cagtcaataa
    13021 ttccatcctt gagagcctgc cacaatgcat ctgcatgacg ctttgtccgc aacggaggat
    13081 tcatctgagc caatgtgccg agctgctcgt aacattccgg tgcagtcaag gtgaaatgct
    13141 gtggacaaac ctctgttgaa atgcgatgct cccttggtaa cgtgcgcaga aaatcacatt
    13201 catcttccgt tgtcaaatgc agaatatgca gtcgtcgccg gtatttaagc gaaaaccgca
    13261 cggcaagttt tgtagccctg agtgccgcag attcatttcg gagcagggtg tgaatgcgta
    13321 cgtccgaaat atccttgagc aatgctttgt ttgcctgaag aacagattcg tcttccgcat
    13381 gtactgctat cagacgggag ccgttggcaa aaattttttc caaaacatcc ggatcgtcca
    13441 ccagcaaatc tccggtagat gaacccatga aaatttttat accacaaaca ttttctactg
    13501 cattcaactc ctccagatta tccggagttg ctcccataaa aaaaccgtaa ttaactacag
    13561 atttttctgc cgccaactgc tttttttcct gcatccgctg acgcgtgatt accggaggtg
    13621 tattattcgg catatccaga aaacttgtaa ctccgcctgc cgcagcggca caagaaccac
    13681 tgtgtaaatc ttctttgtga tcagctcccg gttcacggaa atgaacctgt ggatcaatga
    13741 tacccggaag caaaaacaat ccatccgcat caatcacttc tccaacgtca gggatttcat
    13801 taccaatagc gcaaatccga ccactgataa ctgagacatt tgtccgctcc gttcttaccg
    13861 gaagaactac ctcggcattc cggatagtaa gtgctgtttt catctaaaac ttccataatc
    13921 tgcacggagt aaagcagcag catcctttgc aaaatatgtc agtatcatat ctgcacctgc
    13981 ccgacgaatt gacaatagca tttccatcat agcactatcc cggtcaaccc agcctttctc
    14041 tgcagctgca acaaccatcg cgtattctcc ggaaacatta taagctgcca ctggtgcatc
    14101 aaaggcctca cgaacacgtg aaattacgtc cagatagcac aatgcaggtt tgatcatcac
    14161 cacatccgct ccttcttcca aatctaaagc aatctcctta agtgcctccc ggatgttggc
    14221 aggatccatc tggtatgttt ttttgttacc actttttggt gcagattgaa gagctccacg
    14281 aaatggtcca taaaaactgg aagcatactt tgcagaatat gcaagaatgc cgacctgaga
    14341 aaatccctgc acgtccagcg catttcgaat tgcaccaatt cgcccgtcca tcatgtcgga
    14401 aggcgcaatc cagtctgcac ctgcttctgc atgtgaaagt gccattttgc acagtacttc
    14461 aacagattca tcgttcacga tttcaccatc aatgaccagt ccgtcatgac cgtgagtcgt
    14521 atatggatca agagctacat cggtttgcac acacaaatct ggaacagcag attttattgt
    14581 acgaatagca cgctgaacaa ggccatctga atcatacgac ttggtagcca attcatcttt
    14641 ttcttcagaa tagccaaata agtttactgc accaacaccc agtgcataaa gttcttcaca
    14701 ttctcgaacc agttcatctg tagataagcg aaattgtccg ggcatactgt caatatgatc
    14761 ccggacggat ttgccttccg tcacaaacag cggataaata agttggcgcg gagaaagttc
    14821 tgtttcacgc accaatccac gaataggtgc ggtgcgtctt aaacgtcttg ggcgatacac
    14881 caaatccatt ttaaacctgt caaatgcaaa ttaaatggtc aactgttgaa acactcgacc
    14941 acgagccttc aaccgctatc caaaatgaag gggggaacac tcatacaaaa aattaaagag
    15001 tgcttaaaaa gaagcttatt atcctgccga tttttctatt acaattttgt ttttgcctga
    15061 acgtcgaatg acaatcttat cgcctttatt ccaatccaac tcttgaaaaa tctgttgtgg
    15121 aattatcaca cgcctcgaac ttccggcttt acctgatgct ctaactgtat ccaggtgttt
    15181 acgctttttt ggctttgcaa ctttttttac aactcttgtt tttgcaaact gaggcggaat
    15241 ttttccaagt ttcattgcgg cactcaacaa gcgggccttt aatgtataaa ccggaatttt
    15301 taaagccttt gctgttgcac ccatgttatt tttagaagca agctctgtca cctttcttaa
    15361 aaattctgca tctcttttca tatcaatcct ttttattaga taacatttcc taaatatctt
    15421 tcctgctttt caaaactaag caaacattaa cagtagcggt tgaagtataa ttcaattttt
    15481 ttcaggacac aagaatttat ttaattctgc aacttttttt aatagtctgc ctcatttcat
    15541 gcaggaatct ttttttgact ttcaagctta aacttcactt gtaatttctt aaaatactct
    15601 atttcatatt gatatggtgt gtagtaaata ttcacatttc cacggtctgt atcttcccag
    15661 aattttgctc cagaatactg aagtcttgca aaaatagtat ccattgtttt ggaaaacagt
    15721 ggttctccca ataataaaag atcttgtaga gtatcttttg caattgtttc agcaaactct
    15781 ttttcattct gattgagata aaacaaggcc aaaccaatat gtaaaagccg aatgccgtga
    15841 ttttgactaa agaattgtat cgcaatttct ttgtccatat cttgaaaatt atcaaacaat
    15901 aaaaactttt gcagtaattc ctgctgtagt tttctctccc aattattctg atagattatc
    15961 tgcattagtt tctgcatttc ggaagcaaga atgtctaaaa taaaagctaa ggaacgtgct
    16021 cgtggaattg cgttaaagca ctgttgtcca taaaatacaa aataacctat acacgtctta
    16081 acccggtcca cttgccggtg ttccaccagg tagctgagaa actgtccata atgaaaaaca
    16141 agattatata aattgcgcgg ttcattattg tgctgaccgt gttttagtgc aaatctaaag
    16201 taagtgttaa gacgaaccgt gattacttca atgagttcct ggtcgtcaca ttctatcgcc
    16261 gtttttccca cctggctcaa ttgttccaca cacaaagtag aaagatcgaa ctctccttta
    16321 tcaagaaaga cgttgtatac atttcctatc attctaaagc tcttctgttc ataaaatgtg
    16381 tgagattttt caacttccgc caataaactt ttcaaagtcc ggaaagaaat atcatcatga
    16441 atctgttgat caattttata aaagtcttca ggaaattttg gttttaactt cacgtaatat
    16501 cttagtaatt cgccaattcc ttctataatt tgtgattttg gttctttgta tggaacaaag
    16561 accaacaaat ccattagttg attgagttct tcaaataact ggtactgtat ttttgcgtgt
    16621 tcttccggcg atattattcc tgtgggacct tttttagcta aacgcttaaa cttggcataa
    16681 ctaccctgca gtaagttttc gattacatta gaggttttgg tagatttgag aatggataaa
    16741 acaaaaggga agccaatcac cagtgaaaca gcaagtaaca cgtggtaatt aaatatgagg
    16801 ctggatacta tttcaactcc ggcctccgaa aggatcttga ttgtgaaagc atgtatcaga
    16861 cacgctgcgg cccaccagac aaaaaataga ctgggccaat gatccatgta taaatcaatc
    16921 agtttcggta cattttggga ggcaaatgtg atgacgattg ttagacttcc taagatgatg
    16981 gcaaagaaag aaagccagac tataggagca aaaccgacat caagatctga ccagaccgga
    17041 ttggctccaa ttttaagcag gaaattatta tagagaggaa aaaggtaaat tcgaaaaagg
    17101 gaatcagcaa gcagcattcc tgaaaaaacc atcagagccg catactgtga acgtgaacga
    17161 gtcagcagtg cgcgtagaat tcgcgaaaag gcctgcgtca aattgactga ttcccgcatt
    17221 tcgtttttta gctttcaggt ttcaatgggc aggattccat ttgagtagta ttccgtttat
    17281 gcctgatcgg agtcctcttc caaattttcg gtttgaactc ttttcattgt cagacgtcct
    17341 tcattatttt ccagaatggc ttgaaaagta ggcttatgtt ttagtccggc gcgagctgat
    17401 tcttcaaggc tatacagcga tttagcccgt ttggtcatgg ttataacttt ttcaaacaag
    17461 ttgacctttt catcctgttt gtgttcttcc atcaaattca gataactttc tttcattttt
    17521 ttgtttttaa taattggtta actcgacagt cacgagtttt atcgtgtcat cccgaacgta
    17581 tgtgagggtt gttataaaat atcagcactt tttcagatct ctcccctcgg tcgagataac
    17641 atgctgcagt caatattatc agcaccttac gagatttaat aactcacaaa ctggttaaag
    17701 tgacaaaagt aaaatctcag tttcctgccg aaagacggaa tcaggagctt tttccagcaa
    17761 ctgctcaagc aaattttttg ccttttcagt ttctccttta gcaattgcta tcctggccat
    17821 tgcccgccaa cgtacgtcct cccatgatgc tatgttaatt gaatccagca tcatctctgc
    17881 ttcgtcccac cgctgctgct gttcaaacaa ggccgccaaa cttagccggg ctgcatttcg
    17941 cagaaaagtt gtagcttccg gatgctttat tacatcctga aaaactgcaa ttgactcatc
    18001 aatttgtgtc tgacgtgcat atgcctctcc ggattccatc aatgccagta cgctcagtgt
    18061 agtgcctgat tcagacttgg caaaatcccg taaagcagat attccctgag ttaacctttc
    18121 atcttcagaa agagctggat tggtaagcat tgcccgtgca atatgatatt gattggcctg
    18181 gttaatacgt tcattttgca catacttcca acctccccaa attgccagaa tcaatacaac
    18241 aactgcaact gcagcactga tatacaagcg tttcttccgg tatgcatgat cgacaaatgc
    18301 aaataacttt ttttccagac gatttggctg cagcagctct ttgcggctca tttgttctgc
    18361 atcgctcatt aattttccgt caataactat ttttttgaaa aatgtatgat ttcatttcat
    18421 ttcctaaccc aaagtgaatt aaggtgggcg tccttctttt ctgctgtgag attctcatcg
    18481 ccaatagatt tcatactctg caataaatct caataagaaa gatgcaactt tttagtttaa
    18541 gctatattgc taaacccttc aagcataatt gtgttaaatc accaagaagt cgccatttat
    18601 cacattctac cagaaatttt ccggattaaa ccgttagcgt agaaaaggct tgtttatcag
    18661 actgaggcat agactaggat gtgatactaa atactctgta ctgagcgcaa tcccgttttg
    18721 attttacata accaaaacct tcgcaagttc cgtggcgaat ttagcagtca cagcaaaaat
    18781 agattccata ggatttgcag caattgatgt tggaaacaca gaaccatcaa atattgaaag
    18841 attctccact tcatgatgac gcccttttga attaaccacc gatttagctg cgttttcacc
    18901 cataggacag cctcccataa catgcgctga aaacaaacgg gctttcaaag tttccattgg
    18961 taaagatgca atcgcttttt taactttatc gaggctgtgg tatagcccgg cgtccctgtg
    19021 aacaggagca acagctttgg cgccggaagc aaattggatt tccgacattg ctataaaagc
    19081 atctctgatt cctccccata cgtatccgga aacaggataa tccaaaccgg gagttccgtc
    19141 acttttcagg taaacttttc caccaatgct ttctggatga aatccgtctc gctgaagtgc
    19201 aaccaagacc tgcagaaaag gtcgctgcct catgatttct gcatggactt taccatgtcc
    19261 ctccaaagca gttgcgacca acatcggctg caatggtgga cattccagct tgaaaccgga
    19321 cttttgattg tcgaggcgcg tctcaatgta atggtctgag taacttgttt gcggtgcacc
    19381 gtattctcca ttgaccggaa acggcataat ggctccggaa atattgacag gatgaagaaa
    19441 tgtccgtgtt ccaactaaac cataagggtt taatattttg ttttcagaac gtaacatgat
    19501 ggctggactt ccaattgatc ctgcagccag tacataatgt ctggctttaa tttttatggt
    19561 ttgtacggct ggggcattgc cgcgcggagt catcgctttg catttcagtt cagtaatttt
    19621 tccgtttttg atttcaatag tttccgctct ggcttttgaa atgagagtcg ctccctcttt
    19681 taatgcaccc ggaatagtcg taaccaaagt ggactgtttt gcgttgaccg ggcagcctgt
    19741 tccgcaatat cccaaattaa gacatcccgt aacatttctc ctgatcttcc cccaactaac
    19801 tccgagtttt tcgcaacctg tttccagcgc actattattg gcgttgggag gaaccttcca
    19861 atcctgaata ttgtagcgtt tctccgacca ttcaaaccag ggaagcattt catcctccga
    19921 aaatccaaga acccccattt cctcagccca atattttagt gtttcagcag gtgtgcgaaa
    19981 gcttgttgtc cagttgattc ctgtaccacc gccaaccatt cttccctgaa atattttgat
    20041 gccgcgatct ttggtttgtc ttccggctga ctcttgataa agattggaat aggcttcatt
    20101 ttctttcatg tgaaaatcac gggaagtctg aagcggccct tcttccacga tgatgacgtt
    20161 caatccagat tgagccagaa tttctgcgga atttcctcct cctgctccgg aaccgataat
    20221 cacgacatct gcctcaaggc tttgatccgc agccaaccga gacgcgtcaa tatgtttcca
    20281 tccctgctgg aaaccctctg ccacaaaatc aaggatcata ttttttgaat cactcatctt
    20341 tgactgtctg gtaaaaattc tgaaatccac cccgcacctt aatcagcaat ctggaaatga
    20401 aaaaatagaa taatccggag gctgtctatg tgcggtcatt tctacggtag agagaaatct
    20461 taataatatt agtttagtag ctcatgcaag atttttcgct tcgctcaaaa tgacaaactt
    20521 aaggataact tttagatttg ttacgagttc atcaataatt attgaactta aaacaaaacc
    20581 gggggacccg cataactaca aaagtcccag gaacgtgggt tggcatacca ggatgccatg
    20641 gttaattcta ccatccccat gtagccgact cgaagctcat ttcctgtgat caaactttcc
    20701 ggatcactga ggcgccaatt atttaaacaa tcatctattt cgctgggatc gtcccaagct
    20761 ccaggaactc cggtaagtaa acgtggagtt aaagagaata taaaatttga gtccatcact
    20821 aaaaacagct tccggatttc ctgttgagtc aacggcggca atccgctaac ggcaatatcc
    20881 cagccgacaa ttacttcctt gatcgccaat accctttctt cattagactc gggcagcgct
    20941 tctgcaagaa taacaggggt aaactttgac aagattccac gatcaaccgg agttaaaaat
    21001 tcaaaggaat gacccaatcc gtcaagtgca ggtggaattt ccagagacgc atgaaaagtt
    21061 tcaatcgcca acagggttac tccgctgacc gcacctaatt taaataaatt tctgcgtgta
    21121 agcatgtttt tcaaaataaa atgttttttt gaaaaagaga ggaaaattag aagaataaat
    21181 taagtgaagc aataaactca ggtttctccc tttgtaagtt ttctttgttt ggcctcctgc
    21241 cagagttttt ccagtttttg aagactgaca ttttctggag attgaccatc ttcagccaga
    21301 ctttcttcaa caaaacggaa acgtcgttca aatttagcat tggcgcggcg taaagctaat
    21361 tcaggatcaa catgaacatg acgagcaaga gtggctacgg tcagcaaaat gtcaccgagt
    21421 tcttcttcga tagactcttc ggaaatttca tttgcctcca gttcagaccg taactcctga
    21481 atttcttcgt ttattttttc aaaaaccgaa tctatttccg gccattcgaa tccaactttt
    21541 gcaacccgtt ttcccagttt ttctgctcgc atcaaagccg gaagcgccaa agcgacatca
    21601 tcgagaacgc tcaccgaggc ggcattacca tgacgttgag ctttttccac tcgttcacgt
    21661 cttttttgcg cttcccattc tccactatga aactcttttg aacgttcaga ctccttccca
    21721 aatacatgtg gatgtctaca gatcattttg tcgcttatgg tttgggcaac gtcgtcaaaa
    21781 ttaaagtctc cggattcttc cgccattcgt gaatgaaaaa ctatttgcaa aagcaagtca
    21841 cccagttctt ctttcagcga agacatatct ttgctgtcaa ttgcctcaga aacttcgtaa
    21901 gcctcttcaa tagtgtaagg tgcaacactg gcaaaagttt gttccctgtc ccacggacaa
    21961 ccctcttccg gatgccgcaa acgggccatc accgttttca ggttttcaat tggagttaat
    22021 gcgggtgttt tcttttcatc cactttatgt tttccaggac cagggtagaa gtttttccgg
    22081 acaaaattca cgaatcagag cccgtgctaa aatcaaaccc ggatcaatta aatatgttga
    22141 tccaagttta cgttccggaa gtcccagggg aatttctgtg cagcctaaaa caacagcttg
    22201 tacatcttgt ttttccaatt cctgaactcc ctccagcagc acttttctgg ctgctttgct
    22261 tacaggatgt gcctgtactt tgattccata ttccggatga aacagggctt cgtcgtgtaa
    22321 acgctgctgt tgcggctcct caagtattct gacttcgtaa ccggcttcac cgagcaattt
    22381 cgggtaaaac ccggctttcc aggttccgat tgtggaaagc acaccgacga ttttaacttc
    22441 cggacatgtt tcccttaaaa agtcgactgt ttctgcgatc atatccagca acttgaggcg
    22501 gcttccggat tgtttgagct tttccatgaa tacatcccgg attgcaggtg catgtgcggt
    22561 gttgcaggga aatcccgcaa ctgttgcacc aagatcttct aattcagtta aattacggaa
    22621 aatggcgtgg gcgggattct tagtggtttg ccccaaaaga aaacgggtac ggtctgcaat
    22681 atcggcagga gtggaaattg agatggttgg gagataatcc tgatcaatta ttgcactggt
    22741 ttgctgcaat attttttgtg ctaaatcgag tcctgcatac ggacctgcac ctccaacgat
    22801 gccgattttt gcatcatcac tgctcatggt tttgtctttg atgagaaatt cagaataatg
    22861 gctaacggta gggatcgact agagccatcg tttacgtcgt ctgtagtttt ttccatcacg
    22921 gaagctttta cgaccagcac cacccatgcc taaataaaac tctttgacat cttcattttc
    22981 acgcaattct gctccttcgc catccatcac gacacgaccg ttttcaagaa catatccata
    23041 atctgcataa cgcagggcaa tatttgtatt ttgttccgcc aaaaggaaac taactccttc
    23101 acgttgatta aggtctttga caatttcaaa tatttcttca acaagctgcg gtgcaagtcc
    23161 catcgacggt tcatcgagca ggatcatttt aggctttgac atcaacgccc gtccaatggt
    23221 aagcatttgc tgttctcctc ctgaagtgta gcctgcctga ctggttcggc gctctttcaa
    23281 acgtggaaaa taggaatagg ttttttcaag atcagctgca attgtagatc ttccttcctt
    23341 tcgtgtatag gcacctgtca ataaattttc ttcaacagtc aggtgttcaa aaacatgtct
    23401 gccttccata acctgaatgc atccacgctt cactaattcg ttaggagaaa gatcatgaac
    23461 ttgggtacca tcaaaatcaa tactgccttt ggtgacatcg cctcgttctg cctggagtaa
    23521 gttggagatt gctttaagag tggttgtttt accggctccg ttggcaccca aaatggcaac
    23581 aacactcccc tttgggacat ccagggagac acctttgagt acaaggataa catggtcgta
    23641 aatgacctca atgttgttaa cgcttaacaa gttcttgcca gatgaagtgc tttgatatca
    23701 aaagaaaaaa gggagagttg aaacaacttc tcccttttta agttaatgtt taaaagataa
    23761 tcaggaacag ggtatagtcc tgtcgggtac actgttttta gctttatagt cggccgcggc
    23821 cttttccagc attggcctta ccctgtccat ggaatttatc catccggata ctctttccca
    23881 gcttgtccca ttccactgct ggataaatat gggaccgtta ttcgcatggt catcacaaga
    23941 tactttgatg ggattggcaa atcctggtaa accaatttct gcgagtctgg cagaggtcag
    24001 gttgagattt tccatccccc accgcatttc ttcactgctg ataaccctgt taccaaattt
    24061 accctgtgca gtccgaattg cctctgcaac aagtacagaa ttgaaaactc ctctcatgta
    24121 gaaattttct ccgactcttg aacgatcctt gacctggctt tttcctttat caacaacata
    24181 cttcaaaata tcctgaacgg caggataatt ggttcctatt ccatggaaat ttgctgattt
    24241 atatcctttg gctgctttac cagctggacg ggcatcatct tcacctcctg accaccagac
    24301 gccaacgaat ttttccatat tgaacttaat tcgtgtagct tccttaatgg cagttggatt
    24361 catggctccc caaccccaca tgatcatcca atcaggcttt tcttttctta ctttcagcca
    24421 atgagaagat tggttctgca tttctttacc tgcaacagcg attttaaaca gtataaaacc
    24481 cagttcgctt gaaagttgtt caaataatgg aatgggttct ctaccatagc ctgattcaag
    24541 atagataaaa ccaattttct taccccttaa attactcatt cctccttcct ggtagccgat
    24601 atattttatg atggctgaag cctgattcca gtaagttgag ggataattaa aaacccatgg
    24661 aaagacactg ccgtccgcgg ctgctgaaag tccgtatccc atggagtgca cgggaatttt
    24721 atctacaggc gactttggaa ttaactgtag tgtgatacca gtggaatagg gattgatcac
    24781 tactggattt ttctgtttta ttctttcata acactcaact ccttttttag tgttatatcc
    24841 cgtctcacat tcttctacaa ttataggtac accacctatg cccccgtctc tttcattgag
    24901 catgtttaaa taatcactca tcccatttgc gttgggtatc ccacttccag caaatggccc
    24961 ggttctgtaa gtaaaaagcg gaatataaat cccttttgcc gccattattt cttgagacag
    25021 ccataaacat gttatgactg aagcaactag agtaaggaaa aatttctttt tcataacaac
    25081 tcctatctga tgattcacga ttaaaatgaa ccctggtcca tttgatggat aggatttcat
    25141 ttgagtcaga acaactccga ctcttccatt gagccagaac aactctgacc cttccacggg
    25201 gtggattccc ctaagataaa tcaataggga aatggccatg cccgaagttt ttccctcaca
    25261 attgaccata tttttgccaa accatgaggt tcaaaaataa gaaacagtac aatcaaagca
    25321 cctaatatca taaaattaaa atgttcaacg gttgccgagt ttattggaat tcctaatatc
    25381 agtggaagat ttgtaatcag aattgggata agaactataa atgccgcacc caaaaatgct
    25441 cctgaaatgc ttcctaaacc gccgatgatt accatgaaca aaatttcaaa tgacatgtta
    25501 atgtcaaaga cttctacttc tgcagatccc aaatatgtga acacgaaaag tgctcctgca
    25561 acaccagcat aataggaact aattgcgaat gcaatcaatt ttgtggggaa tggtcgtatt
    25621 cctatcaatt ccgctgcaat atccatatcc ctaatggcca tccaagcacg cccaattctg
    25681 cctctgacaa ggtttacggc aactagtgct aaaaaagtta caatgcataa tacaaccata
    25741 taacgtgcct gcgtggtagc tgtcggacct gttacagcaa tatcaaaaaa atttcttaca
    25801 gggacctcaa ttgcacctga attattatag ttgtaaaacc attgaactct gccaaaaagc
    25861 cattcaaaga aaaactgggc tgcaagagtt gcgactgcga gatagaatcc tttgatcctt
    25921 aaagatggta tcccaaatat taaaccaatg cctgcagaga aaaaacctga aataagaata
    25981 agaacaatga cgtttgtttc cggaaaaaca gtaactaatt tatagcatga ataagcacct
    26041 actgccatga atccacctgt gcccaaagac aactgtccag catatccggt cagcaggttg
    26101 agacctaaag ctgataaaga ataaataaga aaagggatta agatggcttg cagccaatag
    26161 tcgtttgcta tcaatggaac aataataaat gaaaatccaa tgattacaaa tattccccaa
    26221 cggtcctgtt ttaccgggaa aagttcctga tctctgatgt atgttgtttt aaattgtcct
    26281 gcttctcgat atagcatttt taatttattt taacttatac tctttcaata attttctctc
    26341 caaataaccc ctgcggtcgg aagagaagaa aaacaagtgc gatcatgtag gccagccaac
    26401 tttcaatact tccacccact agatcccccc aataaaattc tccaatcttt tcaccgatac
    26461 caatgatcaa tccaccgaca attgctcctt gcaccgatgt gaaaccccct aaaattaaaa
    26521 ccggtagggc tttaaaagca atgatcgaga gggcaaagct gacatcactc ttagcccccc
    26581 acatgattcc cgtaatcaaa gcaattatcc ccgaaatgaa ccagacaaga acccaaattg
    26641 tgttgagaga tacacctaca gaaagagcgg cctgattgtc atctgctact gctcttaaag
    26701 caattcctat ttttgttttt cccaagaata gtgctaagaa aattaatgcc aatgcagcaa
    26761 taccagaagc agctatatct atttcctgca aaatcagcat atcatcaaag aaagtaatgg
    26821 agccgtaagg tagtaaaagc tcttcagtaa tcatcatttt tggttctcca ccaaaaataa
    26881 attcaccaaa acctatcaaa aaaaacgtaa caccgatggt tgccataaaa agtatgatat
    26941 ccggctggtt gactaggggt ctcaaaacaa gcctttcgac tgaaaaagct aatgcataca
    27001 tcaccaatat tgtaatgggc agtgcaatat atgcggggat tccattttca tggagaccta
    27061 caagagttaa tgcggcaaat acaaccataa ttccttgtgc aaaattgaac acaccggatg
    27121 ccttaaaaat tagtacgaac ccaagcgcga ttaatgaata aagaataccc gttacaaatc
    27181 cttcccaaat aacttgcaaa aataaaatgg gatcagctgc catatcaaaa aacggatgta
    27241 tgaatatcgt cgttaaaatt tccatcaaaa cattattatc tattaatttg cagtggtaac
    27301 cccaagatag gcatcaataa cggtctggtt accacggatc tcatcaggag ttccttctgc
    27361 cagtttttca ccataatcca gaacaacaac tttatctgaa atatccatga caacactcat
    27421 gtcgtgttca attaaagcga tggtagttcc atattgatta ttaacgtcca agacgaatcg
    27481 gcacatgtcc tgtttttctt caatattcat ccccgccatc ggttcatcca gcagcagtaa
    27541 atctggttcc attgccagtg ccctccccaa ctcaacacgt ttctgcaaac catatggcaa
    27601 gcgaccaacg ggtgtacgac gaatggcttg aatttcaaga aaatcaataa tgtcctcaac
    27661 gtattcccga tttg
//
LOCUS       DTVD01000122            2126 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_22800_length_2126_cov_421.827137, whole genome
            shotgun sequence.
ACCESSION   DTVD01000122 DTVD01000000
VERSION     DTVD01000122.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22800_length_2126_cov_421.827137"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="EYO68_04435"
     CDS             complement(<1..1333)
                     /locus_tag="EYO68_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIC07087.1"
                     /translation="MPEGLITVVYIVSAALFILSLRGLSNQETARHGNMFGVAGMIIA
                     VVATVLGGQVGSIGTLILVMLIGGGIGLVAARRVAMTAMPELVAILHSFVGAAAVLVG
                     IVSHLESGSLTGTELLIHKIEIVLGVFIGAYTFTGSVIAFLKLRGSLGGKPLTLPLRH
                     QLNILLVLVSVLFGTWFVNGAPEEQIMYLLIIISIAGLLGIHMIMAIGGADMPVVVSM
                     LNSYSGWAAASAGFMLSNDLLIITGALVGSSGAILSYIMCRGMNRSFISVIAGGFGVA
                     EGTVAAVSKEDDGKEAQSISYEETADILRGAKQVIVVPGYGMAVAQAQHIVHEITENL
                     RKRNISIKFAIHPVAGRLPGHMNVLLAEANLPYDIVFEMDEINSEFNATDVVLVIGAN
                     DIVNPGALDDESSPIYGMPVLEVWDAKSVIVLKRSMATGYSGVGNPLYFGDN"
     gene            complement(1400..>2126)
                     /locus_tag="EYO68_04440"
     CDS             complement(1400..>2126)
                     /locus_tag="EYO68_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HIC07088.1"
                     /translation="EMVESMIPGSVIVDLAAEGGGNCEMTKPGEFRVYKKVTIIGFTD
                     LPSRLPTQASQLFSNNTAKLIQHLNVDGELNLDLEDEITSAITVVHDGKVLWDPEAQN
                     KVQKKTVAPVKLETVSEKISELPEVEKKKWSLLKMIGWLSAFVLVALIGFGGSSEFIS
                     HVTVFVLACFVGYMVIWNVSPSLHTPLMSVTNAISGIIVIGGMIHLSGEQIILPAIAV
                     LIAAINIVGGFLVTHRMLEMFRK"
BASE COUNT          639 a          523 c          382 g          582 t
ORIGIN      
        1 tattgtctcc gaaataaaga ggattgccaa ctccagaata tcctgtagcc atacttcgtt
       61 taagaactat cacggattta gcatcccaga cttcaagaac cggcataccg taaatcggac
      121 tgctttcatc atccagcgct ccaggattga cgatgtcatt tgctccaata accaatacca
      181 catccgttgc attaaactct gaattaatct catccatttc aaaaacgata tcataaggca
      241 agttggcttc ggccagcaga acgttcatgt gtcctggcaa acgtccagca accggatgta
      301 tggcaaattt tatactgata tttcttttgc gcaggttttc ggtgatttcg tgtacaatgt
      361 gctgtgcctg tgccactgcc attccgtagc ccggaacaac gataacctgt ttggctccac
      421 gcagtatatc tgcggtttct tcataagaaa ttgattgtgc ttcttttccg tcatcctctt
      481 tagagaccgc agcaaccgta ccttctgcta caccaaagcc ccctgcaatt acactgatga
      541 aagagcggtt catgcctctg cacataatgt aacttagaat tgctccgctg cttccaacaa
      601 gtgctcctgt aattatcagt aaatcattgg aaagcatgaa tccggcagag gcagcagccc
      661 atccggaata gctgttgagc atggagacaa ctaccggcat atctgctcca ccaatcgcca
      721 tgatcatatg aattccgagt aatccggcaa tggagataat tattaagaga tacatgattt
      781 gctcttccgg tgctccgttc acaaaccatg ttccaaacaa aacactgacc agcaccaata
      841 atatattgag ttgatgacgc aatggtaatg tcaaaggttt tccgcctaaa cttcctctca
      901 atttcaaaaa ggcaatcact gaccccgtaa atgtgtaggc accaataaaa acaccaagaa
      961 caatttcaat tttgtgaatt aacagctctg taccagttag gctgcctgac tctaaatgac
     1021 tgacaatgcc gaccagcacg gctgcagctc cgacaaaact gtgcaaaata gccacaagtt
     1081 ctggcattgc cgtcatggct acacgtcgag cagcaactaa tccaattcca ccgccaataa
     1141 gcataactaa tattaaagtg ccaatactgc caacctgccc tccaagaaca gtggcaacaa
     1201 cagcaatgat catccctgca acaccaaaca tgttgccgtg ccgggcagtt tcttgattag
     1261 aaagtcctct caaactgagg ataaaaagtg cagctgacac aatgtaaaca acagtaataa
     1321 gtccttcagg cattattcag tttaatagtt tatggttaaa caacagctgc tatatatttg
     1381 caaccaacaa tatatttgat tattttcgaa acatttccag catacggtga gttactagaa
     1441 aaccgccgac aatgtttata gcggcaatca ggaccgcaat ggcaggcaag atgatctgtt
     1501 ctccggaaag atgtatcatc cccccaatca caattatacc actgatcgca ttggtgacac
     1561 tcatcagcgg tgtatgcaat gaaggcgaga cattccaaat caccatgtat ccgacaaaac
     1621 aagcgagaac aaatactgtt acatgggaaa tgaattctga agaacctcca aatccaatca
     1681 gagctaccaa aacaaaagcc gagagccagc caatcatttt taacaatgac cactttttct
     1741 tttctacttc tggtaattca gatatctttt cagaaactgt ttccagtttt actggtgcaa
     1801 cagttttttt ctgaacttta ttttgtgcct ccggatccca aagaactttt ccatcatgaa
     1861 ccactgtaat tgcactagta atctcgtctt ccagatccag gtttagttcc ccatctacat
     1921 tcaggtgctg aattaattta gcagtattgt tgctgaagag ctgacttgct tgtgttggca
     1981 aacgactggg aaggtctgta aaaccaatga tggtcacttt tttgtaaaca cgaaattctc
     2041 ctggttttgt catttcacaa tttccacctc cttcggcagc caaatctact ataactgatc
     2101 caggaatcat gctctctacc atttct
//
LOCUS       DTVD01000123            8501 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2301_length_8501_cov_450.075657, whole genome
            shotgun sequence.
ACCESSION   DTVD01000123 DTVD01000000
VERSION     DTVD01000123.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_8501_cov_450.075657"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="EYO68_04445"
     CDS             <1..257
                     /locus_tag="EYO68_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HIC07089.1"
                     /translation="SLSAEEQSSPANIFQMFALYEHQIAVLDRKSFGKIGADGKHFLR
                     LSIAAEQSVLEEGILRLKNAGEDTNGLDRFLKKRPDLTSA"
     gene            373..1782
                     /locus_tag="EYO68_04450"
     CDS             373..1782
                     /locus_tag="EYO68_04450"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HIC07090.1"
                     /translation="MQEIHHYDIVVIGSGPAGEKAALQASKLKKQVALIDKSPHLGGA
                     SLHTGTIPSKSLRETVMHLALLRQQTHGIDIRFKENLTTRELMYRKEIVIQDQENSLR
                     RNFEKNQITVIEGIPRFQSATMLEITPADSSEKYEITADYTIIATGSSPRRPKWAPFD
                     NECVFDSDTILDIKHLPKKVTIIGAGVIGCEYASIFSKIGIRVNLIARDRNILPFVDR
                     QIAENLMYQMRNQRVTLRLGENVEDIEKINADEIHVKLESQKILPCSTVLVAAGRFAN
                     SGGLNLDKVGVELGNNGLIKTNENFQTAQPNIFAVGDVIGFPGLASTSMSQARIAVLH
                     AFGQLESEKMPKDLPLGIWTIPAVSMVGKTEEELTDACTPYEIGIAHFKEISRAHIMG
                     EEEGILKLLFDPETLKILGIHIIGPHASELIHLGHSVMFYEGTIQYFMNTVFNSPTLD
                     EAYRIAAFNGMNRLDHESL"
     gene            complement(1807..2805)
                     /locus_tag="EYO68_04455"
     CDS             complement(1807..2805)
                     /locus_tag="EYO68_04455"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HIC07091.1"
                     /translation="MHISRFPRLHFAHLPTPLEPLKNLSKLLGGPQLFIKRDDCTGLA
                     TGGNKTRKLEFLLGEALQKNADTIITQGATQSNHVRQTAAICSKLDLRCEILLEHRTG
                     SKDPDYLENGNVLLDRLFGANILSVPAGTDMDAAMEETAAEIRKNGGNPYIIPGGGSS
                     PTGALGYVNCAMELVGQANDRSLRIDHLVTATGSAGTQAGLLAGLEGTRSGIPVLGIC
                     VNADKETQEVKVFALAEKTAEFLGIPGSIKRESVVANGDYVGPGYGFSTNGMIEAVQL
                     LAREEGILLDPVYSGKGMAGLIDLVRKGKYRKDENIVFLHTGGSAGLFAYRKTFTA"
     gene            complement(2825..3616)
                     /gene="hisN"
                     /locus_tag="EYO68_04460"
     CDS             complement(2825..3616)
                     /gene="hisN"
                     /locus_tag="EYO68_04460"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIC07092.1"
                     /translation="MSEYLKFAHELADASGKVIRKYFRSKMTVENKADDSPVTVADRL
                     TETIMREMISKKFPDHDILGEEHGYQPTGSRWKWVLDPIDGTRSFVAGMPIFGTLICL
                     LEQEIPQIGMIDIPILQERWCGLRKKESIFYPNQPDGKPLACKVSNQQKIEQSILYSA
                     DPAMFNNAQKPYFDQIAALAKLVRFGGDCYSYGLLASGHIDLVVEADLKNYDVMALVP
                     VVESAGGLISDWNGNSSFDDEWDGCLVATASPELHKQALGLLKTA"
     gene            complement(3652..4965)
                     /locus_tag="EYO68_04465"
     CDS             complement(3652..4965)
                     /locus_tag="EYO68_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIC07093.1"
                     /translation="MLKFLGKKGRTLVILILLGALLGIILFLNKILFPFLLASFLAYV
                     LAPVIDNLHQRKIRSHSISRGGAVLLVYFVILVVLIGGGSYVVPKVSAEMGSMIQEFP
                     KAVTKISKKWGPVLDEKISSIASLFPEPDKVEPLPVIEKIEDKNQSTEIWTENGELLK
                     ILNSYTYEIKGLDSDRIEIVPHRKSGSESGIEENPNVPLDMQTQLSLLAEEGVDKLKA
                     NMVRFLGLGRQLISQVVGSVFTLFLTFMVAAFILVDTERTLSFWRSLVPLQYHTRYSD
                     LLKSLDKGLNGVVRGQLIICLVNGILTFIGLMIIGVPFAFTLGLVAMVFSLIPIFGTI
                     LSSIPIVIMGLTISLSTGLLGLLWILIIHFIEGNFLNPKIMGTAAQIHPALIVFALVT
                     GEHMAGIAGALLAVPVYSILQTFFFFLKSMVEELEKEPAVAEKVV"
     gene            complement(5059..6684)
                     /locus_tag="EYO68_04470"
     CDS             complement(5059..6684)
                     /locus_tag="EYO68_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit"
                     /protein_id="HIC07094.1"
                     /translation="MDPKTSKKVSEKETPAMLYDQMLKRGEELLEKPLQGGGESRVRV
                     QHSKGRMTVWERIKVLTTAEAHITFQNWGAELDGAGIVTGILNIDGRDVAVYGHDFTV
                     RAGSMDATNGSKIARLILMAGEHGIPLIGMNDSAGAYVPAGVGGLDGYSEAFSAMRKI
                     SGIVPSILLMFGYNAGGGAYLPRQGSFVIQPENTFFGLTGPDVVREALGEDITADDLG
                     GPKVHSKSGVVDLTAPDELGALRTALRLLRYLPDNNYTAPPFRATSLSMDEYVEEEAI
                     LLHKTFTNPVTGFNTPFDMRLFLQQLVDYGDYFEFQEVRAKQLTTAFGRMGGNVVGFI
                     ANNSAFASGNIDTESSRKGAKFIRFCNLYNIPVIFLEDVSGYAPGSEQERAGVVQAGR
                     ELLDAIVDLRVPRLTLIVRNAFGGAYCAWNSYHVGGDFVFALPSARIAVMGPAGRQYV
                     YKDEFREILKNFQQSLDSGVEEHEAAIVRDKAMAKLTLRYERELLNPEEALRLGSVSS
                     IVMPGHSRKVLGNALCYLLRHYQPSAMGGPQRE"
     gene            6865..7788
                     /locus_tag="EYO68_04475"
     CDS             6865..7788
                     /locus_tag="EYO68_04475"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIC07095.1"
                     /translation="MGTPEWAIPSLQAVLDSGSEISAVFTQPDRRVGRKRVLTTSSIK
                     QFAQNQKLPVHTPEKAGNPETLELVRSLAPELIIVCAYGQILPHSFLDIPRIGCFNLH
                     FSFLPKLRGASPVQAAITYGLETTGVSLQKIVLKLDAGPLIAASYPESIRPDDTTPLL
                     GSRLAEIGGQLIRNTLPKLLEDNFTTSEQNEGEATYCRIIKKDEGRVRWTEETAREIE
                     RKLRAFTPWPGIFTFYSISGKNENKRRIQLTKVEVVNESFESGKVYPELTVGTRTGGL
                     RILRLKPEGKQEMDADSFLRGNPQIVGTVLE"
     gene            7921..>8501
                     /locus_tag="EYO68_04480"
     CDS             7921..>8501
                     /locus_tag="EYO68_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HIC07096.1"
                     /translation="MKEIEFWFSIGSTYTYLSVTRLKEVEQKFEVKFSWQPFSVRKIM
                     LEMDNVPFPPTKKVKADYMWRDIERRARSYGFEAKVPAPYPLNEFDLANRIAVIGMQE
                     GWCSEYVIATYRRWFVAGLEPGSEPNVSESLREIDQDPERVLELAADETIAKAYLSQT
                     EQAQSKRIFGSPSFIVDGELFWGDDRLEDAVNWA"
BASE COUNT         2517 a         1949 c         1741 g         2294 t
ORIGIN      
        1 acagcctttc ggcagaagag caaagttctc cggcaaacat cttccaaatg tttgcactct
       61 atgaacatca gattgcagtt ttggaccgca aatccttcgg aaaaattggt gcagatggga
      121 aacactttct acgtctttct attgcagcag aacaatcggt gcttgaagaa ggaatcctgc
      181 gtttaaaaaa tgccggcgaa gatactaacg gactggacag gtttttaaaa aaacgtccgg
      241 atttgacttc agcgtgagaa cattattgta gtttgttgcc attgtacctg actcatgctg
      301 gaatagataa acttattaga acttttcagc tacgatttgt taacaaatat ccaaaaaaat
      361 aaaagggaat aaatgcagga aattcatcat tacgacatag ttgttatcgg atccggacca
      421 gctggagaaa aggccgcctt gcaggccagt aaacttaaaa aacaagttgc actgattgat
      481 aaatcaccgc atctgggtgg agcctcactt cacacaggca caattccctc caaatctctt
      541 agagaaacgg taatgcattt agcactgctg cgtcagcaaa cacacggaat tgacattaga
      601 tttaaagaaa atttgaccac ccgggagttg atgtaccgta aagaaattgt gatccaggat
      661 caggagaatt ccttacgtcg caactttgaa aaaaatcaga tcacggttat cgaaggaata
      721 ccacgcttcc agtctgcaac catgctggaa atcactcctg cagacagcag cgaaaaatat
      781 gaaattactg ccgactatac gattatcgcc actggatcat ctccacgaag accaaaatgg
      841 gctccatttg acaacgaatg cgtctttgat tcggacacca ttcttgacat caaacatctg
      901 cccaaaaaag taactattat tggtgccggg gtaatcggct gtgagtacgc ttctattttt
      961 tcaaaaatag gtattcgagt taatctcatt gcacgtgacc gtaatatact gccctttgta
     1021 gatcgtcaaa ttgcagaaaa tttaatgtac cagatgcgta accagcgtgt tactttgcgt
     1081 ctgggagaaa acgtagaaga cattgaaaaa atcaatgcag atgaaataca tgtaaaactg
     1141 gaaagccaga aaattttacc ctgctctaca gttctggtgg cagcagggcg ttttgctaat
     1201 tccggtggtc tgaatctgga taaagtagga gtagagttgg gtaataacgg attaatcaaa
     1261 accaatgaaa attttcagac agctcaacct aatatatttg cggttggaga tgtgattggt
     1321 tttccgggtc tggcatccac ctccatgtcc caggcccgga ttgccgtttt gcacgctttt
     1381 ggtcaacttg aatctgaaaa aatgcccaag gatttacctc ttggaatctg gactatacca
     1441 gcagtctcga tggtaggaaa aactgaagaa gagttgactg atgcctgcac tccatacgaa
     1501 atcggtattg ctcattttaa agaaatttcc cgcgcccaca ttatgggtga agaagaggga
     1561 attctcaaac tgctctttga tccggaaaca ctcaaaatac tggggatcca catcattgga
     1621 ccgcacgcct cagaattgat tcacctggga cattcagtga tgttttatga aggtacaata
     1681 caatatttca tgaacacagt ttttaactcc ccaacacttg atgaagccta ccgtattgca
     1741 gcttttaacg gcatgaaccg gctggatcac gaatcactct aaatcaagct ccggacaaat
     1801 cttacttcat gcagtaaaag tttttcgata ggcaaacaaa cccgccgagc caccggtgtg
     1861 tagaaagact atgttttcgt ctttacgata tttacctttt cgtacaagat caatcagacc
     1921 tgccatccct tttccggaat aaaccggatc cagcagaatc ccttcttcac gagccagtag
     1981 ctgcacggct tcaatcattc catttgtcga aaatccgtaa ccaggaccga cgtagtcacc
     2041 attcgcaaca actgattcac gttttatact gccgggaatt ccgagaaatt ctgcagtttt
     2101 ttcggccagc gcaaatactt ttacctcctg agtttctttg tctgcattta cacaaattcc
     2161 caaaaccgga ataccactgc gcgttccttc caggcctgcc aaaagtccgg cctgtgttcc
     2221 ggcactcccg gtagcagtaa ccagatgatc aatccttaaa ctacgatcat tagcttgtcc
     2281 cactagttcc atggcacaat ttacataacc cagcgcacca gtcggactcg aaccgccgcc
     2341 gggaataata tagggattgc cgccgttttt tcttatttca gcagccgttt cttccatggc
     2401 agcatccata tcagttccgg ctggaacaga aagtatattt gcaccaaaaa gtcgatccag
     2461 caaaacattc ccgttttcaa gatagtccgg atctttgctg cctgtacggt gttccagcaa
     2521 aatttcgcag cgtaaatcta attttgagca aattgctgca gtctgccgaa catgatttga
     2581 ttgtgttgcg ccttgagtaa taattgtgtc tgcatttttt tgcagtgcct cccccagcag
     2641 aaattccaat ttacgtgttt tgtttcctcc agtagcaaga ccggtacagt catcgcgttt
     2701 aataaaaagc tgtggaccgc caagtagttt actcaagttt ttgagtggtt cgagaggagt
     2761 cggcaggtgc gcaaaatgga gtcgtgggaa tcgtgaaata tgcatattat tttttgaaac
     2821 aaggttaagc agttttaagc agacccaagg cctgcttgtg taattccgga gatgcagtcg
     2881 caaccaggca accatcccat tcgtcatcaa aagagctgtt accattccag tcgctgatca
     2941 atccgcccgc tgattcgact accggaacaa gtgccattac atcataattt ttgagatctg
     3001 cctcaactac caaatctatg tgtccggaag ccaacaatcc atagctgtag caatctccac
     3061 cgaatcgtac cagttttgcc agtgccgcaa tctgatcaaa gtacggtttt tgagcgttgt
     3121 tgaacatcgc cgggtctgcg ctgtaaagaa tggactgttc gattttttgc tgattggaaa
     3181 ctttgcaggc aagcggtttt ccgtcaggct gattaggata aaatatactt tcttttttcc
     3241 tcaatccgca ccagcgttcc tgaagaattg gtatatcaat cattccaatt tgcggaattt
     3301 cctgttccag cagacaaatc aacgttccaa atattggcat tccggccaca aaactgcgtg
     3361 ttccatcaat aggatccagc acccatttcc atctgcttcc tgttggttga taaccgtgtt
     3421 cttcgccgag aatatcatga tcaggaaatt tttttgaaat catctcgcgc ataattgttt
     3481 cggtcagacg atctgcaaca gtaaccggac tgtcgtcggc cttattttca acagtcattt
     3541 ttgaacgaaa atatttccga attacttttc ctgacgcatc tgcaagttca tgcgcaaatt
     3601 tgagatattc agacatccgg cttttgaaag gggaaaaaat taatggactt tttaaacaac
     3661 tttttctgcg actgcgggtt ccttttccag ttcctctacc attgatttaa ggaagaagaa
     3721 aaaagtttgc agaatggagt aaacaggtac agccaacagt gcccctgcta ttccggccat
     3781 atgttctccg gtaaccaggg caaaaacaat cagagcagga tgaatttgtg ccgcggtacc
     3841 catgattttt ggattgagaa aatttccttc aataaaatga attattaaaa tccatagaag
     3901 ccccagcagt cccgtactta atgaaattgt cagtcccatg attacaattg gtatactcga
     3961 aaggatcgtg ccgaagatgg gaattaatga aaaaaccatg gccacaaggc caagtgtgaa
     4021 agcgaagggg actccaatta tcatcaaacc gataaatgtc aaaatcccat taaccaggca
     4081 aataatcagt tgacctcgta ccactccatt taatccttta tccagacttt tgagcagatc
     4141 actgtaacgt gtatgatact gcaaaggcac aagagaacgc cagaaagaaa gagtacgttc
     4201 ggtatccacc aagataaatg ctgcaaccat aaaagttaaa aacagtgtaa aaactgagcc
     4261 aaccacttgt gaaatcagtt ggcggccaag tccgagaaag cggaccatat tggctttcag
     4321 cttgtccaca ccttcctctg ccaaaagaga taattgagtt tgcatatcta atggaacatt
     4381 ggggttttct tcaattccac tttcgctgcc tgacttacga tgcggaacta tctcgatgcg
     4441 atctgagtca agacccttga tttcataagt gtaactattc agaattttca gcaattctcc
     4501 gttttccgtc caaatttctg tggattgatt tttatcttca atcttttcaa taaccggcag
     4561 cggttccact ttatcaggtt caggaaagag agaggcaata ctgctaattt tttcatctaa
     4621 aaccggcccc catttttttg atattttcgt cacagctttg ggaaattctt gaatcatgga
     4681 acccatttct gcagagactt tgggcaccac ataacttccg ccaccaataa gtacgactaa
     4741 aattacaaaa taaactagca ggactgcacc tccacgagaa atactgtgcg aacggatttt
     4801 tctctggtga agattgtcaa tcaccggagc aagtacgtat gccagaaaac ttgccagtag
     4861 aaaaggaaaa agaattttat tcaaaaacaa aattattccc agcagtgcgc ccagcaaaat
     4921 aagaataacc agtgtacggc cttttttacc aagaaattta agcatataaa atgaaagatg
     4981 taatttaaac tgtagaaaga caaacagatt caaacaaatc aggatacact ttttgtttga
     5041 tttttcaagc tgtttcggct attccctttg cggtccgccc atagcgctag gttgataatg
     5101 acgcaggaga taacacagtg cgttgcccaa aacctttcgg ctgtgcccgg gcattacaat
     5161 actggaaaca gaaccaagcc gcagagcttc ttccggattg agcaattccc tctcataacg
     5221 taaagtcagt tttgccattg ccttatctcg aacaattgca gcttcatgtt cctccactcc
     5281 ggaatcaaga ctttgctgaa aatttttcag aatttctcga aattcatctt tgtaaacata
     5341 ctgtcttcca gccggaccca tcacagcaat tcttgcactt ggcagggcaa aaacaaaatc
     5401 accgccaacg tgataactgt tccaggcaca gtatgcccct ccaaaagcat tcctgactat
     5461 cagcgtcagt ctgggcacac gcaaatccac gattgcatca agtagttccc gacctgcctg
     5521 aacgactccc gcacgttcct gttcagagcc aggagcatag ccactgacat cttcaagaaa
     5581 aataaccggt atgttgtaga ggttgcaaaa tcgaatgaat tttgcacctt ttctggagga
     5641 ttctgtgtcg atgtttccgg aagcaaaagc gctgttgttg gcaatgaaac caactacatt
     5701 gccacccatt ctgccaaatg ccgtggtcag ttgttttgcc ctgacttcct gaaattcaaa
     5761 ataatctcca taatcaacaa gctgctgcaa aaacagtctc atatcaaaag gcgtgttgaa
     5821 accagtaact ggattggtga atgttttgtg gagcagaatt gcttcctctt cgacatactc
     5881 gtccattgat aaagaggttg cacgaaaagg aggcgctgtg taattattgt caggcagata
     5941 acgcaaaagt ctcaaagcag tgcgcaatgc tcccaattca tccggagcag tcaagtcaac
     6001 cacaccgctt ttactgtgaa ctttcggacc tcccaaatca tcggcagtaa tatcttcacc
     6061 aagtgcctca cgtacaacgt ccgggccggt taaaccaaaa aaggtatttt ccggctgtat
     6121 cacgaaggaa ccctgccgcg gcagatatgc tcctcctccg gcattgtagc caaacatcag
     6181 caggatcgaa ggcactatac cgcttatttt acgcatggcc gaaaatgctt ccgaatatcc
     6241 gtcaagccct cccacaccag ccggcacata agctcctgcg gaatcattca taccaattaa
     6301 tggaataccg tgctctcccg ccatcaaaat aaggcgcgca attttgcttc cgtttgttgc
     6361 gtccattgaa cctgcacgaa cggtaaaatc atgtccgtaa accgcaacat cgcgtccgtc
     6421 aatgttgaga attccagtca caattccggc accgtccagt tccgcacccc agttttgaaa
     6481 agtaatgtgg gcttcggctg tagtcaaaac tttgattcgc tcccatactg tcatccgccc
     6541 cttgctgtgc tgaacacgaa cccgtgattc accaccgccc tgcaaaggct tttccagcaa
     6601 ctcttcacca cgcttcagca tctggtcata aagcattgca ggagtttctt tttcagaaac
     6661 ctttttagat gtttttggat ccaatggatt ttcgagattt aatttttcca cagctcagca
     6721 tcaaaataaa aggggccact tcaataaaat taactaaaca gaactttaat cccaaatcac
     6781 ttgcaaagca agttaaacta tagtgttatc agctaaatta taatttatca tttctactgt
     6841 gtaataataa tgagcataat tttcatggga acaccggaat gggcaatccc ttctttacag
     6901 gcagttttgg attccggatc agaaatcagc gccgttttta ctcagccgga caggcgggtt
     6961 ggacgcaagc gggtgctgac tacgtcatcc ataaagcaat ttgcacaaaa tcaaaaattg
     7021 cctgttcata ctccggaaaa agcgggaaat ccggaaacac tggaattggt gagatcatta
     7081 gctccggaac tgattatagt atgtgcttat ggacaaattt taccgcattc ttttttagat
     7141 attcccagga tcggctgttt caatctgcat ttctcttttt tgccgaaatt gcgtggagcc
     7201 tctccggtac aggcggcaat tacttacgga cttgaaacaa cgggtgtatc tttacaaaaa
     7261 attgttttga aacttgatgc aggccccctg attgccgctt cctaccccga aagcattcgt
     7321 ccggatgaca caactccgtt actcggaagc aggctggcag aaattggagg acagcttatt
     7381 cgcaatactt taccaaaatt gcttgaagat aatttcacca cttcagagca aaatgagggg
     7441 gaggcaactt actgtagaat catcaaaaaa gatgaaggtc gagtacgctg gactgaagaa
     7501 acggcccgcg aaattgaacg taaattacgg gcatttacac catggccagg aatttttact
     7561 ttttatagca tatccggaaa aaatgaaaac aaacgcagga ttcagttgac aaaagttgaa
     7621 gtagtgaatg aaagctttga gtcgggaaaa gtttatccgg aattgaccgt gggcacacgt
     7681 accggaggtt tgcgaatctt gcgtcttaaa ccggaaggca aacaggaaat ggatgcagat
     7741 tcatttttac gaggaaaccc ccaaatcgtg ggaacagttt tggaataaca gcttgtaaag
     7801 aggtcaaaat accggttgtt aatttgttga cacttcataa tttgaatgtt acgattcgct
     7861 cagaaattac atcgttgaac tgactaaatg taaatattaa acttcaataa aattaaatcc
     7921 atgaaagaaa tagaattctg gttctccatc ggcagtacct acacctatct ttcggttaca
     7981 cgcctcaaag aagttgaaca aaaatttgaa gtcaagtttt cctggcaacc tttcagtgta
     8041 cgaaagataa tgctcgaaat ggacaatgtg ccttttcctc caacaaagaa agtgaaggcg
     8101 gattacatgt ggcgtgacat cgaacgtcgg gcacgcagct atggttttga ggccaaagtt
     8161 cctgctcctt atcctctcaa tgagttcgac ctcgctaacc ggatagctgt cattggaatg
     8221 caggaaggct ggtgttccga atacgtcata gcgacatacc ggcgctggtt tgttgctggt
     8281 ctcgagcccg gaagtgaacc aaacgtttca gaaagtctca gggagatcga tcaggatccg
     8341 gaaagagtcc tggaactggc tgctgatgaa acaattgcta aagcctatct aagtcaaacc
     8401 gaacaagcac agtcaaaacg tattttcggc agcccaagtt tcatcgttga tggtgaactg
     8461 ttctggggtg atgaccggct ggaggatgca gtgaattggg c
//
LOCUS       DTVD01000124            8476 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2312_length_8476_cov_213.930887, whole genome
            shotgun sequence.
ACCESSION   DTVD01000124 DTVD01000000
VERSION     DTVD01000124.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_8476_cov_213.930887"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="EYO68_04485"
     CDS             complement(<1..440)
                     /locus_tag="EYO68_04485"
                     /inference="COORDINATES: protein motif:HMM:PF04295.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07097.1"
                     /translation="MNQVSALLEKFKLKDRQHIRTLVIQENGGTRKTIENGIKIVRKL
                     LERTRDFQRETVSAKHLCIGLECGGSDAYSGISANPALGAAADLVVEHGGSAILSETP
                     EIYGAEHLLIQRAVTPEVGNRLMDLIHWSSFVVLIIFIHRLLKN"
     gene            complement(461..1048)
                     /locus_tag="EYO68_04490"
     CDS             complement(461..1048)
                     /locus_tag="EYO68_04490"
                     /inference="COORDINATES: protein motif:HMM:PF04295.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07098.1"
                     /translation="MRLHPNDNVGLALTKIKENCSFENVIAQENIPAGHKIALAEIQK
                     GEAIRKYNQTIGFASQTIHAGDHVHTHNIEFHSFERLPEVGGVKNKINKPNKSANFQG
                     YLRSNGKVGTRNYIGILSTVNCSASISQRIAGYFKSESDGESFNDNMAPFPNADGVIA
                     LTHDSGCGMSIEGDGLTLLQRVLTGYAEHPNFAGF"
     gene            complement(1350..2240)
                     /locus_tag="EYO68_04495"
     CDS             complement(1350..2240)
                     /locus_tag="EYO68_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011569824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC07099.1"
                     /translation="MNQSSPGVNLVIYRILIYGALLFWAFLCLFPIYWTITTSFKTAV
                     SVTQGHLIPWIDFTPKWIGFRSLGLSPETIFQISTVREEFLKRFFNSVITSVSASILA
                     VMLGSLAAYGLSRFQYKLGFVKNSDISFFFLSQMILPPVVLALPFLVLYRELALLDST
                     IGLVMLYALMVLPIVIWIMRDQFSTIPIELEEAGLVDGLNVWGVFMRIVLPISFPGMV
                     AAFILCLVLSWNEYFFAALLTSADAKTLPVMVASQTGSQGINWWSMAALSTAAIAPLA
                     IIGVFLESYIVKGLSTGAVK"
     gene            complement(2237..3328)
                     /locus_tag="EYO68_04500"
     CDS             complement(2237..3328)
                     /locus_tag="EYO68_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC07100.1"
                     /translation="MTTTYIGTDHVPAQQKTLGRVIIFIATILLVALFLIQILYKTDT
                     ITLGFENWRPTLYAYLLWSIALCWGILLIKGDRGQRALFVLPAFLFTIAMVIFPLIFS
                     VYISLHDWNLSAFEGQKFNGLDNFRALLVDEYYWNSMLNMVYYLVAILFEYAIAFGLA
                     LLLNSQIVARKFFRVVFLMPLMLSPVAVSWMLGKSLMEYRFGPAATLARHLGWESPAF
                     FSSPEIARFSIMVLDAWTFIPFMMIMLLAGLQALPKEVNEAAKIDGATGWQHFWKITF
                     PLMLPVSVTATVIRIIFKLKLADVVINVTSGGPGGATDTVTSFIFREYRDRSNVGYGT
                     MMAVVYLIIIIILMTLMLKLIDRWMQKVT"
     gene            complement(3489..>4913)
                     /locus_tag="EYO68_04505"
     CDS             complement(3489..>4913)
                     /locus_tag="EYO68_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004143571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC07101.1"
                     /translation="ARVSKGEFDGVELTFMGLNNQNLHNFLFRGFLKPWEKYTGAKIK
                     WIDLAQADYNARLQQSIATKTVDFDILEMGAPFEGDVCGKGLASEMPNWVKDQIEMDD
                     YVGYLKAPVGTWNGKTYRISIDGDCHNFNYRADYFKDAGFAAAWKAEGHKGAWGVPKT
                     WQKVQEVSKFLKGKKDPTFGGDAVGYLDPCKGWGGFGFYFLASRATAYAKHPNDPAWL
                     FDADTMKPRVNNPAWVRAIQDVIDVLPSQPANQLNADPGTTAFQQFLAGTGSMLSWWG
                     DVGSMAKTSDGSVVGDVVGFDILPGSDDVYNSKTGRWDTLPGGPNYAPNMAYIGWGVY
                     VMARVDSNSKKRKAAWSAAAHLGGKDISLWASAYPSGFQPYRNSHFNIDEWVNAGYDR
                     AFITSYLESESNSYNHPNAAIEPRIPGIFQYYSVAEDELSKIYAGKYDVQTGANNIAA
                     AWEKLTDQIGRKKQVALYRASLGL"
     assembly_gap    4914..5013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5213..6283)
                     /locus_tag="EYO68_04510"
     CDS             complement(5213..6283)
                     /locus_tag="EYO68_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIC07102.1"
                     /translation="MLDSRWEQLADILVNYSTSTGPGERVLITMMETDTWPLARAVHS
                     AVIKVGAHPHIEFQSTLLQRDLMQGGDPEQFDSAHELQQKGMQWADVYIGLRGAANPH
                     ELNGIKPERITAFRKSLGKVSALRTEKTRWVLVRVPNAAFAQQAGLSTDEMMEFFFDA
                     TLLDWQEESKRYDAIREFMQTTEEVRIVGTGTDLSFNTTGRKYLIDDGHINMPGGEIY
                     TAPTDDSAEGNISFEFPAVFAGQYIEGIKLSFSKGKVVEASADKNEALLLELLEMDDG
                     AKRIGEFGVGANYGISRYCYDLLFDEKIGGTVHIALGRAYPECGGINQSAFHWDLIKD
                     LREEGVLYLDGKKIIEKGHILI"
     gene            complement(6302..7102)
                     /locus_tag="EYO68_04515"
     CDS             complement(6302..7102)
                     /locus_tag="EYO68_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HIC07103.1"
                     /translation="MSEKIIQFGRKTVVAMVHIPALPGSPDYDAASGMTKIIASVIAD
                     LEALQSGGVDAVMFGNEFDRPYVLKAPPEGLAALASVIGQVKSIIKVPFGVNYLWDPV
                     ATVALAVATEASFAREIFTGVYASDMGLWAPDCAGAARLRASQGRSDLQMLFNINAEF
                     ATSLDTRPLAVKAKSAVFSSKADVICVSGPMTGMGVNQTELREVREALPDTPLLANTG
                     VTIDTVADIFSLTDGCVIGSHLKHNGDTWSAVDPERVRKFMDKVETLH"
     gene            complement(7103..>8476)
                     /locus_tag="EYO68_04520"
     CDS             complement(7103..>8476)
                     /locus_tag="EYO68_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HIC07104.1"
                     /translation="AEQDPNDWWDALGKSVRKAIRDSGISVNQIAAMAVDTTCCSVVP
                     IDDSGNPVRPALIWMDVRSAEQAEQMVATEDDALRINSNGSGPVSAEWMIPKALWIKQ
                     NEPENFDRAVTICEYQDYINLHLTGRLGASINNVSTRWHCDYSREGVPKSLLEKLDLG
                     ELAEKWPQDVFRLGELVGGLTPRAAGHLGLTPDLPVVQGGADAQIGMIGLGVVKPGNL
                     ALITGSSHLHLGLSEKPFHGTGIWGTYADALLPGLHTVEGGQTSTGSVINWLKNLFGE
                     SDYEALNRDASKLPPGSENLIVQEHFQGNRTPHTDPKSRGAFHGLTLKHGREHLFRAA
                     IEGVAFGSELILESMRSNGFFPESIVVSGGATRSELWLQIHSDVSNLPLTLTKNPDAP
                     LLGCAILAAVGAGIYEDIPTAVEQMVQIDRVIEPNSQVHAEYQPFYEAYKASYVGLRN
                     IRNNLEN"
BASE COUNT         2335 a         2002 c         1771 g         2268 t
ORIGIN      
        1 ttgttcttca ataatctatg aataaaaata attaagacta caaaactgct ccaatggatt
       61 aaatccataa gtctgttccc cacctcaggt gtgactgctc tttggatgag caaatgttcc
      121 gcaccataga tttccggagt ttcactcaaa attgcactac caccatgttc tacgaccaag
      181 tctgctgccg cacccaaagc cggatttgca ctgattccgg agtaagcatc agaccctccg
      241 cattctaaac cgatacacag atgttttgcc gaaacagttt ctctttgaaa gtctctagtt
      301 cgctccagta attttcgtac tattttgatt ccattttcaa ttgtttttct tgttccacca
      361 ttttcttgaa taacaagtgt acggatatgt tgtctgtctt ttagcttaaa tttttcgaga
      421 agtgcggaaa cctggttcac ttcacatccc aaaccgacga tcaaaatccc gcgaaatttg
      481 gatgttctgc ataaccagtc aaaacccgct ggagcaaagt aagcccatcc ccttcaattg
      541 acattccgca accactatcg tgggtcaggg ctatgactcc atcagcattc gggaaaggcg
      601 ccatattatc gttgaaagat tctccatcag attcgctttt gaaataaccg gcaatccttt
      661 gtgagatgct ggccgaacag tttactgtac tcagaattcc aatgtaattt ctggtgccaa
      721 ctttcccgtt tgaacgtaag tacccctgaa agtttgcaga tttatttggt ttgtttattt
      781 tgtttttaac cccaccaact tctggaagcc tttcgaatga atgaaattca atattatgtg
      841 tgtgaacatg atctccggcg tgaattgtct gagaagcaaa accgatcgtc tggttatatt
      901 tccggattgc ctcccctttt tgaatttcgg ctaaagctat tttatgtcca gctggaatat
      961 tttcttgtgc aatgacgttt tcaaaagaac agttctcctt tattttcgtc aaggctaatc
     1021 ccacattatc gtttggatgt aatcgcattt tatacactat ctgtctttta atttgtttta
     1081 ggttgtgcgt tatgtgacgt aggcacctga ctgcgaatgg gtggcatctc ctcaatcctt
     1141 tgagtttagt aggaggtgct ggctcgcctc aagtttcatt tcatgttgac cagatataat
     1201 tgtatttcta ctcatattat ttataatgat caattgtggt aagttcgcat ttcgaatcgg
     1261 cttggatgca ctcgatatgg cgtaacccga tcaatgccgc acctacgatg gtaatcttgc
     1321 ttagttcata ccggggtttc taatactctc tacttaaccg ctccagttga aagtcctttg
     1381 acaatataac tttcaaggaa aacaccgata attgccagtg gtgcaattgc ggctgtagag
     1441 agtgccgcca tcgaccacca attgatcccc tgtgatcctg tttgactggc caccatgacc
     1501 gggagtgttt ttgcatcggc actagtcagc agggcggcaa aaaagtattc gttccaggac
     1561 aaaacaagac agaggataaa agctgccacc attcccggaa acgaaattgg taagacgatg
     1621 cgcatgaaaa cgccccaaac attgagacca tcgaccagcc ctgcttcttc cagttcaatt
     1681 ggaattgtac taaattgatc ccgcatgatc cagattacta ttggcaaaac catgagtgca
     1741 tagagcatga ccagaccaat agttgaatcg agcaatgcca actcacgata aagtactaaa
     1801 aacggtaggg ccagtacaac cggaggaaga atcatctggg acagaaagaa aaacgagatg
     1861 tctgaatttt tgacaaatcc taacttatat tggaaacgac tgagtccata tgccgcaagt
     1921 gaacccagca ttactgcaag tatggatgca cttacggaag taattacgct gttaaaaaat
     1981 cttttcagga actcttcccg aactgtagaa atttgaaaaa ttgtttccgg agataaaccc
     2041 agtgaacgaa aaccgatcca ttttggtgta aagtcaatcc atggaatcag gtgaccttgt
     2101 gtaacactga ctgcggtttt gaaggatgtc gtgatagtcc aataaatggg aaacaaacac
     2161 aaaaaggccc aaaacaaaag tgcgccataa atcaggatgc gatatataac gaggtttaca
     2221 cctggcgaag attgattcat gtaacttttt gcatccagcg gtcaataagt tttaacatta
     2281 gcgtcataag tatgatgata ataatcagat aaacgacagc catcatggtt ccgtaaccta
     2341 catttgagcg gtctcgatat tcacggaaaa taaaactggt gaccgtgtcc gtagcaccac
     2401 ccggacctcc ggaagtgacg ttgatgacaa catccgccaa ttttaatttg aaaataatcc
     2461 ggataacagt tgccgttacg ctgacaggaa gcatcaacgg aaaagtgatt ttccagaaat
     2521 gttgccagcc cgttgcacca tcaatttttg cggcttcatt gacttctttc ggcaaggcct
     2581 gcaaaccggc aagtagcata atcatcataa acggaatgaa agtccaggca tctaaaacca
     2641 taatgctaaa acgcgcaatt tccggagacg aaaagaaagc cggactttcc caacccaagt
     2701 gacgcgcgag agtcgccgct gggccaaaac gatattccat caatgatttt ccgagcatcc
     2761 aactcactgc aacagggctg agcatcaaag gcatgagaaa taccacccga aagaattttc
     2821 tggccacgat ttgtgagttg agcaacagtg ccagtccaaa agcaatggcg tactcaaata
     2881 ggattgcaac caaataatag accatgttca acatggaatt ccaataatat tcatctacaa
     2941 gaagagcccg gaagttatcc agtccattga atttttgacc ttcaaaggca ctcaagttcc
     3001 aatcgtgtag agaaatgtaa acgctgaaaa tcagcgggaa aatcaccatt gcaatcgtga
     3061 aaagaaaggc tggcaggaca aaaagtgcac gttgaccacg atcgcctttg atgaggagta
     3121 tcccccagca gagggctatt gaccaaagaa gatatgcata aagggtagga cgccagttct
     3181 caaagcctaa agtaatggta tcagtcttgt ataaaatctg aatcaggaac aatgcgacta
     3241 gtagaatagt tgcaatgaaa ataattaccc gtcccagagt tttctgttga gcaggaacgt
     3301 gatctgtgcc gatgtaagtg gtggtcatct taaagtttgc aagcaaatac tgatgagtat
     3361 ttgtgaataa gaatgctgag ctgtactcag cttttataat ttggtgaaga aataagggaa
     3421 gcgggaaaga ccggcgattt ttactttgaa ccaccggtca aaccattacc tgttatcgca
     3481 tgatctaact acaaaccaag tgaggcgcgg tagagcgcaa cttgcttttt cctgccaatt
     3541 tggtcagtaa gtttctccca ggccgccgca atgttatttg ctccggtctg gacatcatac
     3601 ttaccggcat aaatttttga caactcatct tcggcaacac tgtagtactg aaagatcccc
     3661 ggaattcgag gctcaatcgc tgcatttgga tggttgtagg agttggactc tgactccaaa
     3721 taagacgtga taaatgctct atcatatcct gcattaaccc actcatcaat attgaagtga
     3781 ctgttgcgat acggttggaa tccagacgga taggcagaag cccaaagaga gatatctttt
     3841 cctccaagat gagcagcggc actccaggcg gctttacgct tttttgagtt actatcaacg
     3901 cgagccatga catacactcc ccagccaata taggccatat ttggagcata gttgggtccg
     3961 ccgggcagcg tatcccatct gcctgttttt gaattataga catcgtcaga tcctggaagt
     4021 atgtcgaaac caacaacgtc accaacgaca gacccgtcac tggtttttgc catggaacct
     4081 acatcacccc accaggaaag catcgagcct gttccagcaa gaaactgctg aaaagcggtt
     4141 gtgcctgggt ctgcattcag ttggtttgca ggttgggaag gcagaacatc aatgacatcc
     4201 tgaattgctc ttacccatgc tggattatta acacgtggtt tcattgtatc tgcatcaaac
     4261 aaccatgcag gatcattggg atgcttggca taggccgtag cacgactggc caggaagtag
     4321 aaaccaaaac caccccagcc cttgcaggga tcaagataac cgacagcatc tcccccgaaa
     4381 gtcgggtctt ttttgccttt gaggaattta ctgacttcct gaactttctg ccatgttttt
     4441 ggtacgcccc aggcaccctt gtgcccttcg gctttccagg cagcagcaaa acctgcatct
     4501 ttaaaataat cagcccggta attgaaattg tggcaatcgc cgtcaatcga aatccgatag
     4561 gtttttccat tccatgttcc aacgggagct ttcagataac cgacataatc atccatttct
     4621 atttgatctt tgacccaatt tggcatttcg gaggcaagtc ctttgccaca tacatcaccc
     4681 tcaaacggag cccccatttc caaaatatca aagtccaccg ttttagttgc aatggattgt
     4741 tgcagacgtg cattgtagtc tgcctgtgcc agatcaatcc attttatttt tgcacccgta
     4801 tacttctccc agggtttcag aaatcctctg aacaggaagt tgtgcaaatt ctgattgttc
     4861 agtcccatga aggttaattc gacaccgtca aattcaccct ttgaaactcg tgcnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaacaat catttgcagt accggacagt
     5041 acattttgca gaaggcaaaa tatgacacaa atgtttgctc aaaaccactt ctgcgctaag
     5101 cgcagaagtg taaaaccaat cgcaatgcga ttggataaca accggacaac ccggttgtta
     5161 aaggcttaaa gttttagttt tctcccaagt ataaatataa ttgtgtacca cgctatatca
     5221 aaatatgacc tttttcaatg atttttttcc catccagata cagcacacct tcttcccgta
     5281 aatctttaat aagatcccag tgaaaagcgg attgattaat tccgccacat tccggataag
     5341 ctcgtcccag agctatatga acagtcccgc ctatcttttc atcaaaaagc aaatcataac
     5401 agtagcgact gattccgtaa tttgcaccaa ctccaaactc gccaattctc ttggcaccat
     5461 cgtccatttc aaggagttcc aggagcagtg cctcgttctt gtcggcagaa gcctctacta
     5521 ccttaccttt agagaaactt aatttaatac cttctatata ctgtcctgca aagactgccg
     5581 gaaactcgaa agaaatgttt ccttcggcgc tgtcatctgt cggagcggta tagatttcac
     5641 caccgggcat gttaatgtgt ccgtcgtcaa tcaggtattt tcgtcctgtt gtattgaaac
     5701 tgagatcagt acccgtgccg acaattcgga cctcttccgt agtttgcatg aactccctga
     5761 ttgcatcgta acgtttgctt tcttcctgcc agtcaagcag tgttgcatcg aagaaaaact
     5821 ccatcatttc gtctgtactc aatcctgcct gttgggcaaa tgcagcgttg ggaacccgta
     5881 ccaggaccca gcgggttttt tcggttcgta aggcagaaac tttgcctaaa gatttccgga
     5941 aagctgtaat tctttcaggt tttattccgt tcagttcatg agggttagct gcaccacgta
     6001 atccaatgta aacatctgcc cactgcatcc ctttttgctg aagctcatgt gcgctatcga
     6061 attgttcagg gtcaccgcct tgcatcagat cccgctggag cagggtggac tgaaattcaa
     6121 tatgaggatg ggcaccaacc tttatcaccg ctgaatgtac ggcccgtgcc agaggccagg
     6181 tgtctgtttc catcatagtg atcagaacac gctctcctgg accggtcgag gtcgagtaat
     6241 ttaccagaat atcagcaagt tgttcccagc gagaatcaag cataaaaacg gttttgaaaa
     6301 atcagtgtaa agtttcaact ttatccataa atttccggac tcgctccgga tcaacagcac
     6361 tccaagtatc gccgttatgt ttgaggtgcg agccgatgac gcacccgtcg gtgagcgaaa
     6421 aaatatcggc aacggtgtcg atggttactc cggtattggc taataacggt gtgtccggaa
     6481 gggcctcacg gacttctctc aactctgttt gatttacgcc cattccggtc attggcccgg
     6541 aaacacaaat cacatcggcc tttgaggaaa acaccgcgct tttggccttg acggctaaag
     6601 gcctggtatc aagtgaggtg gcaaattccg cattgatatt gaaaagcatt tgcaaatcgg
     6661 aacgtccttg tgaggccctc aaacgtgcag ctccggcaca gtccggtgcc caaagtccca
     6721 tgtcagaggc ataaacaccc gtgaatattt cacgtgcaaa acttgcttcg gttgcaactg
     6781 ctaaagccac tgtagctact ggatcccaca gataattcac gccgaatgga acctttataa
     6841 ttgatttaac ctgccctata actgaggcaa gtgccgccaa tccttcaggt ggagccttga
     6901 gtacatacgg acgatcgaac tcgttgccaa acattactgc atctactcca cctgattgca
     6961 aggcttcaag atcggcgatt acggaagcga taattttcgt cattccggag gctgcatcat
     7021 aatccggaga accaggcaag gccggaatat gcaccatggc caccacagtt ttacggccaa
     7081 attgtatgat tttttcagac atttagttct ccaaattatt gcggatgttt ctcaagccta
     7141 cataacttgc tttgtatgct tcataaaacg gttggtattc agcgtgcacc tgagagtttg
     7201 gctcaatcac gcgatcaatt tgtaccattt gttcaactgc tgtaggaatg tcttcgtata
     7261 ttcctgctcc aactgcggca aggatagcac atcccagcaa aggtgcatca ggatttttgg
     7321 tgagggtcaa tggcaagttt gaaacatcgg aatggatttg aagccacaat tcggagcgtg
     7381 ttgctcctcc ggaaacgaca atgctctcgg ggaaaaagcc gttactgcgc atggattcca
     7441 aaatcagttc gctgccaaaa gctacccctt caatcgctgc ccgaaaaaga tgttctctac
     7501 catgtttgag agttaaacca tggaaagccc cacgcgactt tggatctgta tgtggcgttc
     7561 gatttccttg aaaatgctcc tgaacaatta agttttctga ccctggagga agtttcgaag
     7621 catcacggtt gagtgcttca taatcggact cgccaaacaa atttttgagc cagttaatta
     7681 cagaaccggt tgaagtctga ccgccttcta cagtgtgcag tccgggtaaa agcgcatctg
     7741 cataagtacc ccaaatgcct gttccgtgaa atggtttttc actcaatccc agatgaagat
     7801 gagaagaacc ggtaatgagc gcaaggtttc ctggtttgac gacaccaaga ccgatcatgc
     7861 cgatttgcgc gtccgcacca ccttggacga cgggcaaatc aggagttaag ccaaggtgtc
     7921 ctgctgctct tggagtaaga cctccaacca attctccgag tcgaaacaca tcctggggcc
     7981 acttttcggc aagttcgcct aaatcaagtt tttccaataa cgattttggc actccttctc
     8041 tggaataatc acaatgccag cgagtggaaa cattgtttat actggcaccc aaacgacccg
     8101 tgaggtgcag attgatgtaa tcctgatatt cgcaaattgt gactgcacga tcaaaatttt
     8161 ccggttcatt ctgcttaatc cagagagcct tgggaatcat ccactctgcc gagactggac
     8221 cactgccgtt actgttgatt cgcaaggcat catcctctgt ggcgaccatt tgttctgctt
     8281 gttctgcact gcggacatcc atccaaatca gtgcaggacg cactggattt ccagaatcgt
     8341 caatgggcac cacactgcaa caagtcgtgt ccacagccat tgccgcgatc tgattaacac
     8401 ttattccgga atcccgaatc gctttcctga ctgacttgcc gagagcatcc caccagtcgt
     8461 tggggtcctg ctcggc
//
LOCUS       DTVD01000125            2097 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_23310_length_2097_cov_409.010284, whole genome
            shotgun sequence.
ACCESSION   DTVD01000125 DTVD01000000
VERSION     DTVD01000125.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23310_length_2097_cov_409.010284"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2097)
                     /locus_tag="EYO68_04525"
     CDS             complement(<1..>2097)
                     /locus_tag="EYO68_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HIC07105.1"
                     /translation="GYQARYLEIDASAETLLLEDDKDLAFTRKVNERYGSSDFLILTY
                     SPYADLLADSTLDSLRKLSAELLELERVESVMSILNVPLLESPPKPIKELIKNVPTLE
                     SPGIDKALAKLEFLNSPIYRDNLVSPDFKTTAVLINLNDDPLYRELLQQRNELRKKAK
                     DGLLSELEQSELENVLIDFKNHRDKMRLVEHKNISQVREIAEKYRGDAKIFLGGASMV
                     ADDLITFIRSDLQVFGSAVFVFLILTLWVIFRQFRWILLPVITCSFSVVTTSGFLGLF
                     GWEVTVISSNFISIQLIITMAITIHLIVRYRELAGLNPNMDQRQLVLDSTIFMARPCT
                     FAVLTTVVGFGSLVLSGILPVMNFGWMMSAGIGVSLFLTFLIFPVLLILMPKAMPNTS
                     FESRFALTKIFADITERHGKTIMFLSVAALMLSIVGASKLNVENSFIDYFKESTEIYQ
                     GMKVIDQQLGGTTPLDLVVELDESVEETLPVKKDTKPEAELEDEEEFDSFEEEFEETA
                     SEAQYWFTAEKMEKIERIHDYLNGLEQTGKVLSLGTMLKIGKTLNSGKSLDNFLLALL
                     YNELPERFRKIILDPYVSIENNEARFYVRIRDSEPNLRRNELLLQIQHDLKTKLDIPE
                     EKVHLANLLVLYNNMLQSLFKSQILTLGVVIISFLIMFIFLFRSFTIALIAIFPNVLS
                     IGVVLGFMGWMGIPLD"
BASE COUNT          634 a          461 c          402 g          600 t
ORIGIN      
        1 tatccaatgg gattcccatc cagcccatga atcccaacac cactccaatt gaaagcacat
       61 ttgggaaaat cgctatcagt gcaattgtaa atgagcggaa cagaaaaatg aacatgatta
      121 agaatgaaat aatgactaca cccaaagtga gaatttgtga tttgaaaagg ctttgcagca
      181 tattgttgta gagcacaagt aaatttgcga gatgcacttt ttcttccgga atatccagtt
      241 tggttttcaa atcatgctga atttgaagta aaagctcatt gcggcgtaga tttggttcag
      301 aatcacgaat acggacataa aaccgagcct cattattttc gatggaaacg taaggatcta
      361 ggattatttt tctgaagcgt tccggaagtt cattgtagag cagagcaagt aagaaattat
      421 ccagagattt tcctgaatta agcgttttac caattttgag catagtaccc agagaaagca
      481 ctttccctgt ttgttccagg ccattcaggt aatcgtggat tctttctatc ttctccattt
      541 tttctgcagt gaaccaatat tgtgcttcac tggcagtttc ctcaaactct tcctcaaagg
      601 aatcaaactc ttcctcatct tccagttccg cttctggttt agtatctttc ttgaccggca
      661 gtgtttcttc cacactctca tcaagttcca ccaccaaatc aagtggtgtt gtaccgccaa
      721 gttgctggtc aatcaccttc attccctggt atatttctgt ggactccttg aaatagtcaa
      781 tgaagctgtt ttctacattc agtttggacg caccgacaat actcagcatt aatgcagcaa
      841 cactgaggaa cattattgtc ttaccgtgac gttctgtgat atcggcaaag attttggtca
      901 aggcaaagcg ggattcaaac gaagtattgg gcattgcctt tggcataagt atcagcagta
      961 ccggaaatat aagaaacgtc agaaagagcg acacacctat tccggcactc atcatccagc
     1021 caaaattcat gactggtaaa attccgctca gaaccagtga gccaaaacca accacagtgg
     1081 tcagtaccgc aaaggtgcag ggtctggcca taaagattgt agagtccaaa accaactggc
     1141 gttgatccat gtttggattt agtcctgcca attcacgata acggacgatt aaatgaatag
     1201 taatcgccat tgtgattatt agctgtatgg aaataaaatt tgaagagata actgtcactt
     1261 cccaaccaaa aagccccaaa aatccactgg tcgtgactac agaaaaactg caggtaataa
     1321 ccggaagtaa aatccagcga aactgcctga aaataaccca gagtgtgaga atcaagaaaa
     1381 caaaaactgc actgccaaaa acctgcaggt cacttctgat gaaggttatt aaatcatctg
     1441 caaccatgct tgcaccaccc agaaatatct tagcatctcc acggtatttc tctgcaatct
     1501 cacggacttg agaaatattt ttgtgctcaa caagtcgcat tttatcacga tgatttttga
     1561 aatcaatcaa cacattttcc aactctgact gttctaattc actcagaaga ccatcttttg
     1621 cctttttacg cagttcattc ctctgctgga gcaactcacg atacaatgga tcatcattga
     1681 gattgatcag cactgcagta gttttgaaat ccggactaac aaggttgtct ctgtaaatag
     1741 ggctgttcag gaattccagt ttagccagcg ctttatctat tcccggagac tcaagggtag
     1801 gaacattttt tatcagttcc ttaattggct ttggaggact ttccagcagt ggaacattca
     1861 ggatggacat tactgattca actctttcca actcaagcag ttctgcactt agtttcctca
     1921 atgagtcaag tgttgaatct gccagtaaat ctgcataagg agagtaagtt aaaataagaa
     1981 aatcgctgct gccgtagcgc tcatttactt tccgtgtaaa ggccagatcc ttgtcatctt
     2041 ccagcaacaa agtttctgct gaagcatcaa tttccaggta gcgtgcttga taaccta
//
LOCUS       DTVD01000126            2090 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_23441_length_2090_cov_348.178870, whole genome
            shotgun sequence.
ACCESSION   DTVD01000126 DTVD01000000
VERSION     DTVD01000126.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23441_length_2090_cov_348.178870"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="EYO68_04530"
     CDS             <1..1060
                     /locus_tag="EYO68_04530"
                     /inference="COORDINATES: protein motif:HMM:PF05987.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HIC07106.1"
                     /translation="AEDEKEQTEFDEELNRQEALNTETAGNEIVPDEESTEEETVLEG
                     VCPLSFEGNGAELFGILMKNLFFNLLTLGIYYPWAKAKQLRYYYGSSRIHGSDFQFHG
                     TGREMFIGLTKALVVLAVLNFIYEGILAGILSESLLFLTVFLYLFIFFLLILIASVGA
                     RRYRMSRISWSGIRFRFLGTFKDTVILVAKGWILTVLSLGFYYPFYLNRFQDFWTTKT
                     TFGNIAFSYDGLGREVFRIWLKGMLLTILTLGIYLFWLKANLQRYFWSHSSFGESRII
                     SKLYGGMLLKETLIFLLIMILTFGIGRAWAVVRYKKFYLGTFSLEGKIDLAAIKQSEA
                     RLTGATGEGMADFFDVEM"
     gene            1063..>2090
                     /locus_tag="EYO68_04535"
     CDS             1063..>2090
                     /locus_tag="EYO68_04535"
                     /inference="COORDINATES: protein motif:HMM:PF01435.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIC07107.1"
                     /translation="MSKTWKALYFDGRTPTHRNVDVQTDRQGVRIEFEDGTTRIWRNE
                     DFSVQQDRSQGPLRLEYGEFPPEILEIADPEFGEVLGKRLPKKSLFLSVGFAMLAVLL
                     VPAIIYWGIPLASGWFAHFIPVSIEKQVGQYVIDEVFPSRIVCKTDAGKQALEKLVSR
                     LVPPDSAYVFQVEVVDSGLVNAIAFPGGNILIFRGLLEKSPSADAVAGVLAHEMQHVF
                     QRHGTENLLNQVALSGLFKLLTVEANAIAETLFAGVRTLSLLKYTRELETEADALALQ
                     LLYQAGVDPVEMLAMFQVLKNHSSSLPESISTHPDMSSRMEKLEIIIDQKTDFVSKPV
                     LSKQSWESL"
BASE COUNT          541 a          401 c          541 g          607 t
ORIGIN      
        1 ggcagaagac gaaaaagagc aaactgaatt tgatgaagag ctgaacaggc aggaagcatt
       61 aaatactgaa acagcgggaa acgaaatcgt tccggatgag gaaagtacgg aggaggaaac
      121 tgttctggaa ggcgtctgtc cgctgtcatt tgaaggaaat ggggcagaac tgtttggaat
      181 attaatgaaa aatttatttt ttaatttgtt gaccttaggg atttattacc cctgggcaaa
      241 agccaaacag ctgcgttact attacggctc cagcagaatt catgggagcg attttcaatt
      301 tcacggaacc ggccgggaga tgttcatcgg tttgacgaag gctttggtag ttttggcagt
      361 tttgaacttt atctacgaag gaattcttgc tgggatttta tcagaaagtc tcctgttcct
      421 gacggttttc ctttatttat tcatcttttt tctgcttatc ctgatagctt cggttggagc
      481 aagacgttac cgcatgagcc ggatttcgtg gagtgggatc cgtttccgtt ttctgggtac
      541 tttcaaagac actgtaatcc ttgttgcaaa gggatggatt ttgaccgtgc tgagtttggg
      601 attttattat ccattttatc ttaaccgttt tcaggacttt tggaccacaa agaccacttt
      661 cggaaacatc gcgttcagtt atgacgggct gggcagggaa gttttccgga tttggctcaa
      721 aggcatgctg ctgacgatac tcactttggg catttatttg ttctggttaa aagcaaatct
      781 gcagcgctat ttctggagcc attccagttt tggtgaatcc cgaataattt cgaaactgta
      841 tggtggaatg ttgttgaaag aaacactgat ttttttactg attatgattc ttacattcgg
      901 aattggacgg gcatgggccg ttgtccgcta caagaaattc tacctgggaa cttttagcct
      961 ggaaggaaaa atagatctgg ctgcaatcaa acagtctgaa gccaggttga caggtgcaac
     1021 cggagaagga atggcggatt tctttgatgt ggaaatgtag aaatgtcaaa aacatggaag
     1081 gcactttatt ttgacggccg cacgccaact caccggaatg tggatgttca aacggacaga
     1141 cagggtgtca ggattgagtt cgaagacggt acgacacgga tttggcggaa tgaagacttt
     1201 agtgtccagc aggatcgttc gcagggtcct cttcgactgg aatacggaga attccctccg
     1261 gaaatactcg agattgccga tccggaattc ggggaagttt tgggaaaaag gctgccaaaa
     1321 aaatccctat tcttatctgt gggatttgcc atgctggcag tgctgctggt tccggcgatt
     1381 atttactggg gaattccgtt ggcttccggt tggtttgcgc attttattcc ggtatccata
     1441 gaaaaacagg ttgggcaata tgtgattgat gaagtgtttc caagtcgcat agtttgtaaa
     1501 acagatgcgg gaaaacaggc attggagaaa ctggtttcac gtttagttcc tccggactcc
     1561 gcatacgttt ttcaggtaga agtagtggat tcaggtctcg tcaatgctat tgcttttccg
     1621 ggcggcaata ttttgatttt ccgcggactg cttgaaaaaa gtccctctgc ggatgcagtt
     1681 gccggtgtac tggctcatga gatgcagcat gtttttcagc ggcacggtac ggaaaatttg
     1741 ctgaaccagg ttgcattgtc cggacttttt aagctgctga ccgtagaagc aaatgcaatt
     1801 gcggaaacgc tttttgcggg tgtccggaca ctttcactgc tgaaatatac ccgtgaactt
     1861 gaaaccgaag ctgatgcttt ggccctgcag ttgttgtatc aggccggagt ggatcctgtg
     1921 gaaatgctgg caatgtttca agtgctgaaa aaccattcct cctccctgcc ggaaagcatt
     1981 tcaacacatc cggatatgtc ttccagaatg gagaaactcg aaattataat tgatcaaaag
     2041 acggattttg tgagcaagcc agttctttcc aaacaaagtt gggaatccct
//
LOCUS       DTVD01000127            2082 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_23584_length_2082_cov_306.860385, whole genome
            shotgun sequence.
ACCESSION   DTVD01000127 DTVD01000000
VERSION     DTVD01000127.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23584_length_2082_cov_306.860385"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="EYO68_04540"
     CDS             complement(<1..197)
                     /locus_tag="EYO68_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07108.1"
                     /translation="MNPAGHTIRSLIDQHAKTSPDRPFVKYPESGISHTWSEFQARVQ
                     SIARYLKNMDFPPNVPVAGLLG"
     gene            complement(245..1639)
                     /locus_tag="EYO68_04545"
     CDS             complement(245..1639)
                     /locus_tag="EYO68_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC07109.1"
                     /translation="MTLTSVETVVGKAQTAQAEIETTSQKTVDELITGLAWAILEPKT
                     NRSLAEQAVRDTGLGNADDKIIKNHRKTLGLLRDLKHAPSCGIVRKLPEQGLVEIARP
                     VGVVGAVTPSTNPIATPLNKTLNAIKGRNAIILSPSPKGEAVCQRVVDLLQDVLRSLG
                     HPENLIQKLPAPVSKEATHELMQLVDLVVVTGSQNNVRASYRSGTPAIGVGAGNVSSI
                     IDETANLVSAARKITASKTFDNATSCSSENSVVIVEDVYDKAIAALESEGGTLLSPEE
                     TQKLQNTLWIQGRLNPDLIAKSAPEIAEAIGLQRVEMNNVKMLMVEETGTGCEFPFSG
                     EKLCPVLTVYRASNFETACEQVREIYDFQGKGHSVGLHSTDESRPLQIGLNLPACRVI
                     VNQAHCFATGGSFDNGLPFSLSMGCGTWGGNSISENLNYRHYLNIVRIVHPIPPVEPS
                     EKEIFGEYWNKHGR"
BASE COUNT          555 a          437 c          437 g          653 t
ORIGIN      
        1 ccgagcaaac ctgcaaccgg aacattcgga ggaaaatcca tgttctttag ataccgtgca
       61 atcgattgta ctcttgcttg aaattcgctc catgtgtgac tgattccgga ttcaggatat
      121 ttcacaaatg gacgatccgg agaagtttta gcatgctgat caatcaaact gcgaatcgtg
      181 tggcctgcag gattcatact ttcaatagat ttggattttt ttattaagca ttttcatttt
      241 taatttaacg tccgtgtttg ttccaatact ctccgaaaat ttctttttca cttggttcaa
      301 caggtggaat tgggtgtaca atacggacaa tgttcaggta atgtcgatag ttgagatttt
      361 cacttatgga attaccgccc catgtaccgc agcccattga gagtgaaaac ggcaatccgt
      421 tatcgaaact gcctccggtg gcaaaacaat gtgcctggtt cacaatcaca cggcaagcag
      481 gaagattcaa ccctatttgc aaaggccggg attcgtctgt tgagtgtagt cccactgaat
      541 gccccttgcc ctgaaaatca taaatttccc ggacttgttc acatgccgtt tcaaaatttg
      601 aggcacgata cacggttaac accgggcaca gtttttcgcc ggaaaaaggg aattcgcacc
      661 ccgttccggt ctcttcgacc attagcattt tgacattatt catttctaca cgttgaagtc
      721 caattgcttc cgcaatttcc ggagccgact ttgcgattaa atccggattg agcctgccct
      781 gaatccacaa agtgttttgc agtttttgtg tttcttccgg actcagcaga gtaccgccct
      841 cgctttccag agccgctatt gctttgtcat acacatcctc tacaatcacc acgctgtttt
      901 cagaagaaca actggtagcg ttgtcaaatg ttttggaagc agtgattttt ctcgcggcag
      961 aaactagatt agctgtttca tctatgattg atgaaacatt gcctgcgccc actccaatag
     1021 ccggtgtacc gctgcggtag gaagcgcgga cattgttttg tgaacctgta accaccacaa
     1081 gatcaaccaa ttgcattaat tcatgagtcg cttctttgct gaccggtgca gggagtttct
     1141 ggatgaggtt ttccggatgt cccaaactgc gtaaaacatc ttgcagcaaa tccacaactc
     1201 gctgacaaac cgcttcaccc tttggcgagg gtgagagtat aattgcattt cggcctttga
     1261 tagcgttaag agttttgttt agcggagtgg ctatgggatt ggtggaggga gtaactgctc
     1321 ccacgacacc gacgggacgt gcaatttcta ccaagccttg ttcaggaagt ttcctgacga
     1381 ttccacagga aggtgcgtgt ttcaaatcac gcaaaagtcc cagcgtcttg cggtgattct
     1441 tgattatttt gtcatctgca tttccaagtc cagtatctcg aacagcctgt tccgcaagtg
     1501 aacggttcgt tttcggctca agaattgccc aagccaatcc tgtgattaat tcgtcaacag
     1561 ttttttggct ggtcgtttca atttcagctt gtgccgtttg tgctttacca acaacagttt
     1621 caacagaggt tagagtcatt ttttgctttc gaggtggcca aggataactt agcttgcaga
     1681 gaagcgacag tagaaattta aaattatatg ttcgagattg ttgagaaatt cctcaagtta
     1741 aactttggtg ctgcgtccag aatattttca acaaaacttt aggaaacaaa ttcaactgtg
     1801 agtctcagga gcacttatac cttttgcact ttgttaatcg aatatgtctt aaattcaact
     1861 agttgatctc atttttcaag ctttaatata aattaaataa attaagttaa tgtcgtgtcc
     1921 taatgttttt ttcttgacat taatttaaat tgtgatagat atgtggatga aattatcaca
     1981 tagtgtgata atggttataa ttatttataa tattcagtgg agtttcgaga tgaataaaat
     2041 taaattaaaa aaactggcta aatttggttt tgctacaatg at
//
LOCUS       DTVD01000128            2081 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_23601_length_2081_cov_558.972853, whole genome
            shotgun sequence.
ACCESSION   DTVD01000128 DTVD01000000
VERSION     DTVD01000128.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23601_length_2081_cov_558.972853"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="lipA"
                     /locus_tag="EYO68_04550"
     CDS             <1..807
                     /gene="lipA"
                     /locus_tag="EYO68_04550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIC07110.1"
                     /translation="SAANLKLSTVCEEANCPNIGECWNGGTATFMLMGDTCTRGCRFC
                     AVKSAKSPGALDAEEPKKLAETVNKMDLKYVVLTTVDRDDLPDQGAAHIARCIRSTQR
                     ACPDMLIEMLMPDFQGKTELVQQVINARPAVLAHNLETVRSLTPIVRDARAGYDQSLQ
                     VLRYLKQNCPQGYTKSSLMLGVGESRAETLRAMEDLRDVGVDFLTIGQYLQPTKKHLK
                     VEKFVPPEEFAELAELGDKMGFEYVASGPLVRSSYKAAEFYIERKIRGRA"
     gene            804..>2081
                     /gene="aroA"
                     /locus_tag="EYO68_04555"
     CDS             804..>2081
                     /gene="aroA"
                     /locus_tag="EYO68_04555"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIC07111.1"
                     /translation="MSLPDKLTLSPIQKISGSVVLPGSKSLSNRILLLSMLAEGKTEI
                     QNLLDSDDVRRMVEALETLGIQLEENRAENLITVSGTSGIIPVKEATLMLGNAGTAIR
                     PLTAAMTLGHGRFVLDGVQRMRERPIIDLVNGLSQLGANLRCINGTDCPPVEVIADGL
                     PGGITRLSGAISSQYLTAILLAAPYADKEVQIEITDKLVSVPYVEMTLRLMRSFGVSV
                     NHENFRLFHIPRQTYRSPGSIFVEGDASSASYFLAGAAITKGTVTVKGCGTDSLQGDA
                     RFAEVLEKMGAKVEWEPQQVKLTGNSLNGIDVDMNQMPDAAMTLAVAALFASGPTAIR
                     NIYNWRVKETERLQAVSTELRKLGAEVEEGYDYLVIQPPEQIRKAEIDTYDDHRMAMA
                     FSLAACGESPITINNPGCVSKTFPDYFEVLNGLC"
BASE COUNT          569 a          462 c          541 g          509 t
ORIGIN      
        1 agtgctgcaa atcttaagct gagtactgtt tgtgaagaag caaattgtcc aaacattggt
       61 gaatgctgga acggcggcac ggcaactttc atgctgatgg gcgacacctg tacacgcgga
      121 tgcagatttt gtgcagtcaa atccgcaaaa agtcctggag cactagacgc agaagaacca
      181 aagaaattgg ctgaaactgt taataaaatg gatttgaaat atgtggttct gacgactgtt
      241 gaccgtgacg atctcccgga tcagggtgct gcgcatattg cccgctgcat ccggtctact
      301 caacgagcct gtccggacat gttaattgaa atgctgatgc cggattttca gggaaaaaca
      361 gaactggttc aacaagtaat aaacgcacgc cctgcggttt tagcacacaa cctggaaacc
      421 gtccggagtc tcactccaat agtccgggat gcacgtgcag gttatgacca gtcactgcaa
      481 gttttacgat acctcaaaca aaactgtccg cagggataca ccaaatcttc actgatgctc
      541 ggcgtgggcg aaagccgcgc agaaactttg cgggccatgg aggatctgcg tgatgtcgga
      601 gtggattttc ttaccatagg tcaatatctg cagcccacaa aaaaacattt gaaagttgaa
      661 aagttcgtgc ctccggaaga gtttgctgaa ctggctgaat tgggcgacaa aatgggattt
      721 gaatatgtgg cctccggacc gttggtgcgc agttcataca aagcagctga attttatatt
      781 gaacgaaaaa tccggggccg cgcatgagtc ttccggacaa actcactttg tctccgatcc
      841 agaaaatttc cggcagcgta gttttaccgg gttccaagag tctctccaac cgaattttgc
      901 ttttgagcat gttggctgaa ggaaagactg aaattcagaa tctgctggac agtgatgacg
      961 tccgccgaat ggtggaagca ttggaaacac ttggaattca gctcgaagaa aaccgtgcag
     1021 aaaatctcat aacagtttca ggaacatctg gaataattcc ggttaaagaa gccactttga
     1081 tgctgggaaa cgccggaaca gcaatcaggc ctttgaccgc agccatgact ttgggacacg
     1141 gacgttttgt tctggatgga gtgcagcgca tgcgggagcg ccctatcatc gatttggtaa
     1201 acggcctttc ccagctcgga gcaaatttgc gttgcataaa cggaacagat tgtccgccgg
     1261 tggaagtgat tgcagacggc cttcccgggg gaattacccg tttgagcggt gcaatcagca
     1321 gtcaatattt gacagcaatt ctgctggccg cgccttatgc agacaaagag gtgcaaattg
     1381 aaataaccga taaactggtt tctgtgcctt acgtggaaat gacgctgcgt ctgatgcgga
     1441 gttttggtgt ttctgtgaat cacgaaaact tccgtctttt ccatattccc cgtcaaacat
     1501 atcgctctcc cggaagcatt tttgtagaag gcgacgcctc atccgcctct tattttctgg
     1561 ctggtgctgc aattaccaaa ggaactgtga cggtaaaggg atgcggtact gacagcttgc
     1621 agggggatgc acgttttgct gaagtcctgg aaaaaatggg agccaaagtt gaatgggaac
     1681 cacagcaggt taaactgacc ggaaactctt taaacggaat tgatgttgac atgaatcaaa
     1741 tgccggacgc ggccatgaca ttggcagttg cggctctgtt tgcttccgga cctacggcca
     1801 tccggaacat ctacaattgg agggtaaaag aaaccgaaag gctgcaggca gtttctacag
     1861 aattacgcaa acttggagct gaggttgagg aaggttacga ttatcttgtc attcaaccgc
     1921 cggagcaaat ccggaaggct gaaattgata cctacgatga ccacagaatg gcgatggctt
     1981 tttcgctggc agcctgcgga gagtctccaa taacaataaa taatcccgga tgtgtgagca
     2041 aaacttttcc ggattatttt gaagtgctta acggtttatg t
//
LOCUS       DTVD01000129            2080 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_23617_length_2080_cov_211.979259, whole genome
            shotgun sequence.
ACCESSION   DTVD01000129 DTVD01000000
VERSION     DTVD01000129.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23617_length_2080_cov_211.979259"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="EYO68_04560"
     CDS             <1..170
                     /locus_tag="EYO68_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HIC07112.1"
                     /translation="GAKGVGEIGVVGSIPAIANAILDALWERGVRTFDMPAFPQKIWN
                     LLQNVIKNSNE"
     gene            249..533
                     /locus_tag="EYO68_04565"
     CDS             249..533
                     /locus_tag="EYO68_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HIC07113.1"
                     /translation="MTTHFIAHDAADTVGVVVVEQVKAGQEATGWIMETDETVTIKAL
                     VEVPLGHKLALADIKNEDTIIKYGNDIGRAVSDIPKGGYVHVHNVKTKRW"
     gene            533..1705
                     /locus_tag="EYO68_04570"
     CDS             533..1705
                     /locus_tag="EYO68_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005504396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactarate dehydratase"
                     /protein_id="HIC07114.1"
                     /translation="MENWQQSFFGFRRENGRVGVRNHVLILPVDDISNAAVEMVGHNI
                     KGTLAIPHSYGRLQFGADLELFFHTIIGTGRNPNVAAVVVIGIEPGWTQRVVDGISQT
                     GKPVSGFSIEGEGDLATVAKASRKAQEYVQWATELQREECPLSDLWISVKCGESDTTS
                     GLGSNPAVGNLMDKLDPLGVHLCFGETSELTGAEQVCANRSSTPEAKEKFLNTWNAYN
                     DFILENKTSDLSESQPTKGNIAGGLTTIEEKAFGNLQKIGKKTNFIDVLEPAEEPTKG
                     PGLYFMDTSSAAAECVTLQAAAGFTVHLFPTGQGNVIGNPIEPVIKLTANPNTAATMS
                     EHIDLDVSGILKREMNLDQAGSELVNITVRTANGRHTCAESLGHREFVLTKLFRSA"
BASE COUNT          650 a          378 c          475 g          577 t
ORIGIN      
        1 tgggtgcaaa aggagtcgga gaaattggag ttgtaggttc aattccggca attgccaatg
       61 caattttaga cgcattgtgg gagcgaggtg tacgcacttt tgacatgcct gcatttcctc
      121 aaaaaatatg gaaccttctt cagaacgtga tcaagaattc caacgaataa atatgttgac
      181 tctacaaaat ataaaaacta aagttttgta aagttttaaa cctaaacctc aaattaataa
      241 ataaagaaat gacgacacat tttattgcac atgatgccgc agacacagtt ggagtggtgg
      301 ttgtagagca agttaaagcc ggacaggaag ccacaggttg gattatggaa actgatgaaa
      361 cagtaaccat aaaagcactt gtagaggttc cgttagggca caaacttgcc ctggcagata
      421 taaaaaatga agacactata attaaatatg gaaacgacat tggacgtgca gtgtcggaca
      481 ttcccaaagg cggttatgtg catgtccata acgtaaaaac aaagaggtgg taatggaaaa
      541 ttggcagcaa tctttttttg gttttcgacg cgaaaatgga cgcgtgggtg ttcgtaatca
      601 cgttctcata cttccagtag acgacatttc aaatgcagct gttgaaatgg tcgggcacaa
      661 tatcaaaggg acacttgcaa ttccacatag ctacggacgt ttacaatttg gtgctgatct
      721 ggaattgttt tttcacacaa ttataggtac aggccggaat ccaaatgttg ctgcagtagt
      781 cgtgattggt atcgagcctg gttggactca gcgtgtggtt gatgggattt cgcagactgg
      841 aaagccggtg tcaggattta gtatcgaagg agaaggcgac ctcgctactg ttgccaaagc
      901 ttcccgaaag gctcaggagt atgtccagtg ggcaactgaa cttcagcgtg aagaatgtcc
      961 gctttctgat ctatggattt ctgttaaatg cggagaatct gataccactt ccggacttgg
     1021 atcaaaccct gcagtcggca atttaatgga caaacttgat ccgcttggtg tccacctctg
     1081 cttcggtgaa acttcagaat taaccggggc tgagcaggtc tgcgccaatc gttcgtcaac
     1141 cccggaagct aaagaaaagt ttctcaacac ctggaatgcc tacaatgact tcattcttga
     1201 aaacaagacc agtgatcttt ctgagtctca accgactaag ggtaatatag ctggtgggct
     1261 gacaactatt gaggaaaaag catttggcaa tctacagaag attggaaaga agacaaattt
     1321 tattgatgtt ttagagcccg cagaagaacc aactaaaggt cctgggttgt atttcatgga
     1381 tacttcctct gcagcggcag agtgcgtcac gttacaagca gcggctggct tcacggtaca
     1441 tctcttccct accggtcagg gcaatgtgat cggtaatcct attgagccgg tgataaagct
     1501 tacggcaaac ccaaatactg cggcgactat gagtgagcat atcgatcttg atgtatcggg
     1561 tattttaaag cgcgaaatga acctagatca agctggtagt gaactggtaa atattacggt
     1621 tagaactgcg aatggacgcc acacatgtgc ggagtctctt gggcaccgtg agtttgtgct
     1681 gaccaaactg ttccgaagtg cctgatgctc cattctcctg gctagaaata ggaataccat
     1741 ttttacggaa aatgcttagt gaaatgagga taattttgtt aaataaacct cactttcctt
     1801 tgaagcacct aaaatgaggt gaaggataag aaaagattta tccagtagta ttttgtttga
     1861 catagaaact gaaaagatat agtatttcga ataattagtc agggaatgct taatcttttt
     1921 gagaatcttt gtcaatcaaa aaaatgaatt ctattgacac tatattttag attcataaaa
     1981 tattagttga tatcttgact aaaaattgat taaataactg attattgctg aaataatgtg
     2041 ttttaaaata aaaatagtca tatatattat gtaatatcat
//
LOCUS       DTVD01000130            8376 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2370_length_8376_cov_389.267276, whole genome
            shotgun sequence.
ACCESSION   DTVD01000130 DTVD01000000
VERSION     DTVD01000130.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_8376_cov_389.267276"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="EYO68_04575"
     CDS             <1..234
                     /locus_tag="EYO68_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07115.1"
                     /translation="FLLLQGETDALIPVQETQAFWHALQSKNRRHSALLTLPLIEHAF
                     DIFPTLTAQCIVPTIERYLFLLHENYNNQQGKK"
     gene            231..1049
                     /locus_tag="EYO68_04580"
     CDS             231..1049
                     /locus_tag="EYO68_04580"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIC07116.1"
                     /translation="MNTNAKQIHPASAAAEDLVLQELDNGVATLTLNRPRQFNALSEA
                     MLTSLQEKLERITENNAVRLVILQGAGKVFCAGHDLKEMIATRKETYYKTLFKQCSRM
                     MMTLNQMPQPVIARVHGIATAAGCQLVAACDLAVAAEDTRFATSGINVGLFCSTPAVP
                     VSRNIPRKKAMELLLTGDFIDANTALSWGLLNRVAPLEKLDEEVKQLADSILAKSSVA
                     VSTGKKMFYKQLESKMEDAYAFASEIMACNMMADDVTEGVDSFINKRQAVWKGR"
     gene            complement(1199..2065)
                     /locus_tag="EYO68_04585"
     CDS             complement(1199..2065)
                     /locus_tag="EYO68_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIC07117.1"
                     /translation="MEKRIVVPIDYSDVSKDVALFADKWAVRTIGKLYFLHVSRLPQV
                     SYYPGHFEYLDQRDENEDLYTLENFLGQIGLKSNFEFSHEYGSPDLKIVDLVESLEAD
                     LVILSAHSHTMLGRLFLGSNTDYVMHHVHCPIYIFKKTSLQTNKIILVPLDFSEANRP
                     VVEKANLWAERTEYELHFMHVLIPVDYSYFGAETSLGLGKAELEMTEEQGMEAMDNFL
                     APMKITVPEKRLVLFGNSSYSRILEYQKEVQAGLLMLAGHDHTVAGRLFLGSNTDYLL
                     HHIDVPMYVYKC"
     gene            2604..>2854
                     /locus_tag="EYO68_04590"
     CDS             2604..>2854
                     /locus_tag="EYO68_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase FdhD"
                     /protein_id="HIC07118.1"
                     /translation="MNLQKNNYRPEMTNAGIEASYPVTVMDEFGNKRETHITGERPLT
                     IYVDKREIVTLMTLGKYPELLVIGYLHNQGFIKNSEELKA"
     assembly_gap    2855..2954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3561..4250
                     /locus_tag="EYO68_04595"
     CDS             3561..4250
                     /locus_tag="EYO68_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07119.1"
                     /translation="MNGADQHKEEVLERLKTVFESSGKSSRAFSKSIGLKPTSFHKVL
                     TGTAGLTIPLANSIELNHGFRSEWLLSGNGKMKVNKHNQLSPLERCLLEVSLSSIQKW
                     HLLEILIIEKINKRISDQFWGTLRDDSNLQSGEDRRTTAYNNLEQITKVFRELREEEK
                     ACLENQDLIGQKIFTQLTQALLLAAFYGEEWDSIKNNCEEYHDLETDGNLKDFEKLLA
                     YINELLSEIDS"
     gene            complement(4427..4966)
                     /gene="mobB"
                     /locus_tag="EYO68_04600"
     CDS             complement(4427..4966)
                     /gene="mobB"
                     /locus_tag="EYO68_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIC07120.1"
                     /translation="MILKSPPLLGFCAYSGTGKTTLLTQLIPVLKEHGLKIGLVKHAH
                     HGFDTDLPGKDSYKLRKAGACEMLVASAKRWALVHESTERTGDPVLEELLPHLSLEEL
                     DLVLVEGFKHESLPKIELHRPSLGKPYLFPNVPGIIALATDLAPREDIEIPVLDLNKI
                     DEIAEFIMGKYTGRQTSYT"
     gene            complement(5025..5690)
                     /locus_tag="EYO68_04605"
     CDS             complement(5025..5690)
                     /locus_tag="EYO68_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07121.1"
                     /translation="MNEPLLVFREIEKSFSGSQILCGVDLSLYPGKCILLSGKNGVGK
                     TTLLKIIAGLEIPDLAKIEISGKTNCWKKAVRSVRKEIIYLHQHPIMFSSTVESNVAY
                     GLRFASLNRKQRRESVEEALEWAGLTDVAKKQAKTLSGGVQQRVAFTRAWILKPKVLL
                     LDEPMANMDHESREQTYQLLVRMKSAGMSLVITSHFMQYFEGIVDQHFQLKNGALELK
                     SKS"
     gene            complement(5687..6388)
                     /locus_tag="EYO68_04610"
     CDS             complement(5687..6388)
                     /locus_tag="EYO68_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC07122.1"
                     /translation="MNGLLLTTQQALQLLFTGDPELWSIVLLSFSVSLKAILLTTPFA
                     FLVSFALSHGKFPGIRIILAIFNSLLSVPTVVIGLFLYVLLTRQGPFGDLRLLFTQTA
                     MIIGQMILCFPTLVALGYAAIQAADRRVWETARTLGAPPWFSMLTVFFEVRFALLAAV
                     LTGFGRIISEIGISMMIGGNIMGYTRNITTAIAMETSKGEFAQGIALGLVLLLLSFLL
                     TALLSYLQGHKEFAA"
     gene            complement(6390..7229)
                     /locus_tag="EYO68_04615"
     CDS             complement(6390..7229)
                     /locus_tag="EYO68_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07123.1"
                     /translation="MSINIVIRFDKFLVKKYLLWFLFCYLNSGIIQAEPIRLATTTST
                     DNSGLLREIIPHFEQKTGNVVHVLSVGTGHALRLGQDGNVDVVLVHAPNAEMHWVEAG
                     YGVNRRSVMVNDFVILGPENDPAGIRGERNAVKTLQKIARGKHIFVSRGDDSGTHKKE
                     MALWKKTRMKPSVTWYRETGQGMGRVLQMADELNAYTISDRGTWLVLRSKLSLKLLFE
                     GDPELHNLYSIIAVNPSRYPGINYMGAMSLIAWLTSVDGQNQIRKFKLDGQSLFFPTA
                     ILD"
     gene            complement(7219..>8376)
                     /locus_tag="EYO68_04620"
     CDS             complement(7219..>8376)
                     /locus_tag="EYO68_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIC07124.1"
                     /translation="AEEILTVESCGRILAQDINSPANVPNFDRSAMDGYALDAESTFG
                     ASAYNPLMFKVVGEVTPGEIYEAVIQPGEALRIMTGGPVPKGANAVLMAEHAELFDDQ
                     IQVLEAVTPGKHVGQIGEDVKKNQNLLKQGRFLRPQDIAVLASVGLKKVKVVRKPEVE
                     LLITGNELLKPGEKPSGVQIIDTNSVMLRPLIQRDGGVLKAVLHLQDERDLLRRHLSD
                     STADLICMTGGTSVGIEDHGPTLVEELGELLVHGIPMRPAAPTGFGLIAGSSAGSKKK
                     VFLLPGNPVSCLSAYDYFVGRSLRLMGGRSEEWPYRSKMVKLATKISSQIGRIEYVRL
                     RIENERAFFIAAGGASILSSTTQADGFLLTEEESEGFAEGEEVRVWLYDEY"
BASE COUNT         2491 a         1831 c         1593 g         2361 t
ORIGIN      
        1 tttttactgc ttcaaggaga aactgacgct ctgattcctg tacaggaaac acaagcattt
       61 tggcatgccc ttcagtcaaa aaatagacgt cattcagcat tgttgacttt gcctttaatt
      121 gaacatgcat ttgacatttt ccccacatta acggcacagt gtatagtacc aacgatagag
      181 cgctacctgt ttttgcttca cgaaaattac aataaccagc aaggaaaaaa atgaacacaa
      241 acgcaaagca aatccaccca gcttctgctg cagcagaaga tctggtactt caagaactgg
      301 acaatggtgt ggccacttta actttgaatc gccccagaca attcaatgct ttgtctgaag
      361 caatgctgac ttcgctgcag gaaaagctgg agagaatcac agaaaacaat gcagtcagac
      421 tggtgatttt acaaggtgca ggcaaagtgt tctgtgccgg acatgatttg aaagaaatga
      481 ttgccacacg gaaagaaacg tactacaaaa cgctctttaa gcagtgcagc aggatgatga
      541 tgacactgaa tcaaatgcct cagcctgtca ttgccagggt ccatggaatt gctactgcgg
      601 caggctgtca attggtggcg gcctgcgatt tggcggtggc ggcagaggac actcgttttg
      661 ccacgtccgg aatcaatgtt ggtttgtttt gctctactcc ggcagttccg gtgagccgaa
      721 atataccgcg caaaaaggca atggaattgt tgcttactgg cgatttcatt gacgcaaaca
      781 cggcactcag ttggggcctg ctcaaccgtg tggcaccgtt ggaaaaactg gatgaggaag
      841 ttaaacaact tgcagactca attcttgcca aatcctccgt tgctgtttcc acaggcaaaa
      901 aaatgttcta caaacaactg gaaagtaaaa tggaagatgc atacgccttt gccagtgaaa
      961 tcatggcctg taacatgatg gcggatgatg tgactgaggg agtagattct ttcatcaaca
     1021 aacgtcaagc agtctggaag ggacgctgag acatggagtc aacaaacaca ctgaaccaat
     1081 agtgacaatt gaagtaaaaa aagtttagtt cgtgattgcc ctggcccctt gctctcagat
     1141 aaactgatct aacagaaaac taaattactg cattctacaa gaaaactact gccaggtttc
     1201 agcatttgta aacgtacatt ggaacgtcaa tatgatgcag cagataatcg gtattgctgc
     1261 ccagaaacag tctcccggca acagtatggt catgtccagc cagcatcagt aaacccgctt
     1321 gaacttcttt ttgatattcc agaatcctgg aataagaaga atttccaaaa agcaccagac
     1381 gtttttccgg aactgttatt ttcattggtg ccagaaaatt atccattgct tccattccct
     1441 gttcttccgt catctccagt tctgccttgc ctaatcccaa actggtttct gcaccaaaat
     1501 atgaataatc cacagggatc aacacatgca taaaatgcag ttcatactcc gtgcgctctg
     1561 cccacagatt agctttttct acgacaggac gattggcttc actgaaatca agaggaacca
     1621 atataatttt gttggtctgc aaagaagtct ttttaaagat gtaaatggga caatgaacat
     1681 gatgcatgac ataatcggtg ttgctcccca ggaacagcct gccaagcatt gtatgtgagt
     1741 gtgctgacaa aattacaagg tcggcctcta aactttccac taaatccaca attttgagat
     1801 cgggactgcc gtattcgtga gagaattcga agttcgattt caatccaatc tgccccagaa
     1861 aattctcaag tgtgtacaga tcctcattct catcacgctg atccaggtat tcaaaatgtc
     1921 cgggatagta tgaaacttgc ggcagtcgac tcacatggag aaaatataat ttccctatag
     1981 tgcggactgc ccatttgtca gcaaatagtg cgacgtcttt gctgacatca ctatagtcta
     2041 tggggacaac aattctcttt tccatcttac cctcccttat tttttctaag aactccgctc
     2101 aattatcctt agtactttga aagtgcaggc ggatatattt tttcttatct ttcctatcat
     2161 atggactaac tgttattgtg gtacatcttc ttgtcaaaaa acacaatctt ttttccagat
     2221 aaagcgttta cggcagccca aggatttcac gcagaccttc ctgccgagga tattatagca
     2281 agattgctgg aattgaattt aggttgcgta taagaattgg aacagttccc cccggattcc
     2341 ggatctgcgc tgtgcttcac ccggaatgac aatggagaac aatataaagt atcaaaacca
     2401 ttattctctt aatgtaatct ccatggaaaa ggaactaaaa caacagatgt gcgcggcttt
     2461 tgtgcttttt cagggaatac ccttgtagtt aacatcttga acagaataat tacttaatga
     2521 tattattgtt taagattatt tttcaacaag tcctaattaa gcactaaagc cctcaagaga
     2581 acacttaata attgggagaa tctatgaatc tacaaaaaaa caattaccgg cccgagatga
     2641 cgaatgccgg tatcgaagcc tcatatccgg ttacagtgat ggatgaattt ggaaacaagc
     2701 gtgaaaccca cataaccgga gagcgtccgc tgacaattta tgtggacaag cgggaaatag
     2761 taactctaat gactcttggg aaatatccgg aattgctggt aatcggctat ctgcacaatc
     2821 agggtttcat taaaaattct gaagaactaa aagcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnttaagc acttaagtga aaataatttc atgatccgct ataatactta
     3001 cgttagactg caaagtgact tctgttgata gaaaatttat tttacctata tcaaaatttt
     3061 gtgtttcatt attcggcttg atattgttat attcagcaat tagactatgc taaattaaat
     3121 ttcgtgtagt ataataacac attttgttaa aataggactt gctgataaat aattttatcc
     3181 aataatatca ttaagtaatt attcgattga tgatgacaac tgcatggtgt ttcctgaaaa
     3241 agcacgaaaa ccctgcactt ctgttgtttg agcccctttt acgaccaggt aacctcacga
     3301 gaacaatagt tgttcttctt tcgattcttc tcctttgtca tccctagcga agcgcagatc
     3361 cggaatccct gggaaatgta tcaaattttg tactcaaact gtacggtaat ctagaccccg
     3421 gatcaagtcc aggatgacaa aagaaaaaca ttatcagtaa gacctaaata tccatggaaa
     3481 ctgagcggtc aaactcaata gctaagaaat atttcattgt agccagcaga ataattaatt
     3541 tgaaactcgg ataatttagc atgaatggag cagatcaaca taaagaggag gttctcgaac
     3601 ggcttaaaac agtctttgaa tcttccggaa aatcaagccg tgcgttttcc aaaagtattg
     3661 gtctgaaacc aacttctttt cacaaagtat tgactggaac tgcaggcctg actattccac
     3721 tggccaactc tatcgagtta aatcatggtt tccgctctga atggctgttg tcaggaaacg
     3781 gaaagatgaa agtgaacaaa cacaaccagc tatcacccct agaacgatgt ttactggaag
     3841 tttcactttc cagtattcag aaatggcacc tccttgaaat attaataatt gagaaaataa
     3901 acaaaagaat ttctgaccaa ttctggggta ctctccggga tgattcaaat ttgcagtctg
     3961 gtgaagaccg cagaacgact gcatataaca atctggaaca aataacaaag gttttcagag
     4021 aactaaggga agaagaaaaa gcctgccttg agaaccagga tctgataggt caaaaaatat
     4081 ttacccaact aactcaagct ctgttgttag ctgcttttta tggggaagag tgggacagta
     4141 taaaaaacaa ttgcgaggaa tatcatgatt tggagacaga tggaaactta aaggattttg
     4201 aaaaattact tgcttatatt aatgaattac tgagtgagat tgattcataa aatccgttgc
     4261 taagactatt agcaataata ttggttagca attattccaa ataatttatt gtctgttaat
     4321 taaattgtat tattattcga catataatta tattttttgt acattttttt tgattaatta
     4381 tttaataccg tattaatatt ctacagaatc ggaatattct agaaaactat gtgtaggagg
     4441 tttgtctgcc agtatacttt cccatgatga actctgcaat ttcatcaatt ttatttaaat
     4501 ccagaacagg aatctcaata tcttctcttg gagctagatc tgtggctaag gcaattatgc
     4561 ccggaacgtt cggaaataaa tacggtttac cgagactggg cctgtgtagt tcaatctttg
     4621 gaagtgactc atgcttgaat ccttctacca aaaccaagtc cagttcctcc aatgacaagt
     4681 gtggtagaag ttcttcaaga acaggatccc cggttctttc cgtagactca tggaccagtg
     4741 cccagcgctt tgctgaggct accagcattt cacatgctcc tgctttacgt aacttgtagc
     4801 tatcttttcc cggtagatca gtatcaaacc cgtggtgtgc atgtttgacc aagccaatct
     4861 ttagtccatg ttctttcagg actggaatca attgagtcag tagtgtagtt tttcccgttc
     4921 cgctataagc acaaaaccct agtagtggag gcgattttag tatcattttt tgaaatctcc
     4981 agattcaaat tattagtaga ttcatagttt attaatcttt tactttaaga tttactcttg
     5041 agttccagag caccgttctt gagttgaaaa tgctgatcga caattccttc aaaatattgc
     5101 ataaaatgac ttgtaatcac taatgacatg cctgctgatt tcattctgac caataattga
     5161 tacgtctgtt cacgcgactc gtggtccata ttggccatcg gttcatccag taacaaaact
     5221 ttcggcttca aaatccaggc acgggtgaaa gcaactcgtt gctgaactcc tccggacaaa
     5281 gtctttgcct gtttttttgc tacatccgtc aatccggccc actccagcgc ttcttccaca
     5341 gattcccgac gttgctttct gtttaaagaa gcgaagcgta acccataagc aacgtttgat
     5401 tctacagtac tgctaaacat gattggatgc tgatgcagat aaataatttc tttacgaaca
     5461 cttcttacag cttttttcca acagttagtt tttccagaaa tttcaatttt cgccaaatct
     5521 ggaatttcaa ggccggcaat gattttcagt aaggtagttt tcccaacacc atttttgcca
     5581 gacaataaaa tacattttcc cggatacaga gacaaatcaa ccccacatag aatttgactt
     5641 ccagaaaacg atttttctat ctcccgaaaa acaagcagag gttcgttcat gctgcaaact
     5701 ccttatgccc ttgaagatag ctcaataaag cggtcagcag aaaagaaagt aacaataaaa
     5761 caagtccgag cgcaattccc tgggcaaact cacctttact ggtttccatg gcaatggcag
     5821 tagtaatgtt gcgtgtgtaa cccatgatat ttccgccaat catcatcgaa ataccaatct
     5881 cggagataat tcttccaaag ccggtaagta ctgcggccag caaggcgaag cgaacttcaa
     5941 aaaatactgt caacatgctg aaccacggtg gtgctcccaa ggtacgtgct gtttcccaca
     6001 cccgcctgtc tgccgcctga attgctgcat aacctaatgc taccagtgtt ggaaaacaaa
     6061 gaatcatttg ccctattatc attgcagttt gtgtgaataa caacctcaaa tcaccaaaag
     6121 gcccctgcct cgtcagcaaa acatacagaa ataatccgat aacaacggtg ggtacagaaa
     6181 gcaaagaatt aaagattgca agtataattc tgattccagg aaattttcca tggctcaacg
     6241 caaatgaaac cagaaatgca aaaggagtgg ttaacaaaat tgctttaagg gagacagaaa
     6301 aggaaagcag aactatcgac cacaattccg gatcgccggt aaagagcaat tgtaaggctt
     6361 gttgagtagt taaaagcagt ccgttcatct cagtctaaaa ttgcagtagg aaaaaacaaa
     6421 gactggccat caagtttaaa tttgcggatc tgattttgac catctactga agtcagccag
     6481 gcaatcaggg acattgcacc catataattg attcccggat agcgggaagg attcactgca
     6541 ataatgctgt aaagattgtg taattcagga tctccttcaa acaacaattt gagtgacaac
     6601 tttgagcgga gcaccagcca tgttccccta tcagagattg tataagcgtt taattcatcg
     6661 gccatttgca gtacccgtcc catgccctgt cctgtttctc gataccacgt tacagaaggt
     6721 ttcatccggg tttttttcca gagtgccatc tcctttttat gagtccctga atcatcaccc
     6781 ctagatacga aaatgtgttt accgcgtgca attttttgaa gtgttttcac tgcatttcgc
     6841 tctccccgaa ttcctgccgg atcattttca ggaccgagaa taacaaaatc attgaccatc
     6901 acactccgtc gattcacacc atatcccgct tctacccagt gcatttccgc attgggtgca
     6961 tgaaccagaa ccacatccac atttccgtct tgccccaatc tcagggcatg tcctgtgccg
     7021 acggacagca cgtgtactac attccccgtc ttttgttcaa aatgcggaat tatttcccgc
     7081 agcagtcctg agttatcggt gctggttgtg gtggctaaac ggattggctc tgcctgaata
     7141 atcccactgt ttaaataaca gaacaggaac caaagtagat attttttcac aagaaattta
     7201 tcaaatctga ttacaatgtt aatactcatc atagagccac acccgtactt cttctccttc
     7261 agcaaatcct tcagattctt cttctgttag cagaaatccg tctgcttgtg ttgttgaaga
     7321 aagtattgat gctccaccgg ctgcaataaa aaacgccctt tcattttcaa tccggagacg
     7381 cacatattca atcctgccga tttgagagga aattttagtg gcaagtttca ccattttact
     7441 gcggtatggc cattcttcag aacgcccacc catcaacctc agtgaacgcc ccacaaaata
     7501 atcataagct gataaacatg aaacaggatt acccggaagc aaaaatactt ttttcttgct
     7561 gccagcggaa cttccggcaa tcagtccaaa tccggtggga gcggcgggcc gcattgggat
     7621 tccgtgtacc agtaattcgc caagttcttc aaccaaagtt ggtccatgat cttcaatacc
     7681 aaccgacgtt cctccagtca tgcaaatcaa atctgctgtg gaatcactca aatgtctacg
     7741 gagcaaatcc cgctcatcct gaagatgaag gactgctttc aaaactcccc catcacgctg
     7801 aattaacggt ctgagcataa ctgaattggt atcaataatt tgtactccac ttggtttttc
     7861 tcctggtttt aacaattcat ttccggttat taacaattcc acttccggtt tccgtaccac
     7921 tttaactttt ttcaggccta cactggccag cacggcaatg tcctgtggac ggagaaacct
     7981 accctgcttg agcaaatttt ggttcttttt aacatcttct ccaatctgcc ccacatgttt
     8041 tccaggtgtc actgcttcca atacttgaat ttggtcgtca aataattctg catgctctgc
     8101 catcaataca gcatttgcac ccttgggaac aggaccacct gtcatgattc gcaatgcttc
     8161 acccggctga attacggcct cataaatttc tccgggagtg acttctccga caactttaaa
     8221 cattaacgga ttgtaagcag atgcgccaaa agtggattcg gcatccagtg cataaccgtc
     8281 catggctgaa cgatcaaagt ttgggacatt tgcaggagaa ttaatatcct gtgccaggat
     8341 ccgtccgcat gattccactg tcaagatttc ctctgc
//
LOCUS       DTVD01000131            8365 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2373_length_8365_cov_426.756197, whole genome
            shotgun sequence.
ACCESSION   DTVD01000131 DTVD01000000
VERSION     DTVD01000131.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_8365_cov_426.756197"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="EYO68_04625"
     CDS             complement(<1..138)
                     /locus_tag="EYO68_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIC07125.1"
                     /translation="MNDLKQKIRTIPDFPAPGILFRDITTLLADPGAFNDVIERFVEH
                     YQ"
     gene            441..2336
                     /locus_tag="EYO68_04630"
     CDS             441..2336
                     /locus_tag="EYO68_04630"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13145.4,HMM:PF13624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07126.1"
                     /translation="MIQELREYSNNLFFKLLMGVIAITFVLSFGVGGFFGDRKEVVAI
                     VNDQEILLKEYRETYQNRMRAFQEQFGENAEKFAEQLNLRQQVFNQLIDRHLLLTDAA
                     ELNLLATDLELQDFIRRQAFFQKNGQFDYDTYETVLSQNRIVRHEYEGSLRADLLLAK
                     KQQLLGTGLVISSREVEQAYRMDFEEIEVEYVFFDPQIFIDKTTVNQVDLRRYHREHP
                     DEFQTLNQFKIEFYTLSADYYKDIVKVREREVRRYFKKNTESYVTPPQIKARHILLKL
                     PPDSPKETLTEKQQQLEKLLTQIKSGTSFEELAREHSEDGTAADGGDLGWFKPGEMVP
                     AFETAAFALAAGEVSDIVQSPFGLHLIKVDELKEEITKSLDEARDEITGILAESRAQK
                     RLDEDLDRLSGLAGESFTEEARKLSKEVQISKWFDKSQVIPGLGSASGLVTELLSRKP
                     GEMGVWRRNPILGHVVYRLSQAKEPVTRDFEEAKEDVETAVRLEKARSLALETAKTAL
                     TQVKGGENIERLADKLGLKTETLAFTANTRFLPGVGDNTEFRKVGLHLNQNQLFGLSL
                     NENRADLIHFKKRTLAVENADEQKDKVRAQLRQNLQQALLSKELKRLRDSASIEVINP
                     VFRTPGA"
     gene            2425..6246
                     /locus_tag="EYO68_04635"
     CDS             2425..6246
                     /locus_tag="EYO68_04635"
                     /inference="COORDINATES: protein motif:HMM:PF04257.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07127.1"
                     /translation="MPLLHYSNRLECLIVPLAQELEKRDPFDSAEIVVPNFSLEKWIS
                     LKLAQFQGIAANLRFITLEKAINEGLQKKLSGRFYALLKSETTQCLLLEVLRKKLKTS
                     DPLWLPVRSYITPKTKLSPEAGEHRLYQLAGRLTHLFMEYELSRNDEILTHWLAGRNA
                     FDQGSLGTESWQRELWSELFGPEGKVTRHNRSARQSESVEFQPELYTLPQLCRLYSSE
                     SKQQNSILYSQSSNNKLKPLHIFGVSYLSSFHQKALTEQLANLRDIHVYTLNPCMEFW
                     EDVQSFGESKAAARRSLEAERQRFEKYEVLSETEIEMGELFQDEEDNPFLQAWGRPGR
                     ENIKLLNQWSAWCFEPWFADADSSAAQTENSEKPQQSVLNQLQQDILFREPRRAEPLN
                     LEQDESLVVLACANPRREVEAVASLIWDWIRRDPDLQLNECALIVHDMELYQHEIEQV
                     FESIYNLPYHLIDGISGSASRLEDAANSLLGLCFTEYTRRDIFSLINNPCFLQKFDDT
                     VSAGKNSYGEPLQIQQWLQWADELNVFYGIDRKSQEEQGYLHLEHDIYHWEQAFQRLT
                     LGEMVSAEEGNGIFSNGDQQIVPVDLAEEWSAEAARFMLIVRSLIADTRDLPKWKMCG
                     KDWGRYLQTLLKTYLRPIEEADEEAFQNLLRNVLAFRDLDLGDESGPRQFSFSTIFEF
                     FKQKQQSATVHRGHYLAEGVTVSSFKPMRPIPFKAVFLLGMGEGLFPTPYRRDNLDLR
                     HIPVNLNPAVEGQAIRERRLGDVSVTERDRYMFLETLVSTRKHLVLSYVSRNDRTDDE
                     LNPSSIIQTLIDELDSGYLKAKYEIIRHPLKPYSLEYFPELSAEQIDFADNQNPASKT
                     HYPNYDPAAFYQARAFRMRELFDQEFPSSGSSGGFQRISPDWLSPEVMLPITAGAFRI
                     KATESVAVKQLSSVAFSRLRKFLESPLQSTAGHLLGLAEDEEDVSEKVDEPLVLVRLS
                     EWLLLRKVWNMGLNVPAKGSQQKSHPEWQKLYRLQAQRMELQGEMPTGIFGEAMQIRH
                     LRILKNWQKQLTAVLETDWNSLQKNLSQFYFGAVRKGKFNEGLTGTDRILPAVNIQKE
                     NPEPSDSENMYFKIQGETEWWYTDESGNRYVIYFSERESKDKAWLRHFLDVLVLLAVD
                     IIPEGTQVTGLCITGEGKRKSRKIHLPSRQQAQDYLANLLQEMNTEHAAVLMPVESVL
                     ELAKENLTGADYNSRYTEWMDAKLNSSSENLGISSQYGPVKFLTDIAYPENPHQLMNR
                     RFQLFFETVLV"
     gene            6261..7079
                     /gene="xdhC"
                     /locus_tag="EYO68_04640"
     CDS             6261..7079
                     /gene="xdhC"
                     /locus_tag="EYO68_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HIC07128.1"
                     /translation="MENHLKKMQALLEEGTSFVSATLVEIKGSTPRAQGGRMLVTASG
                     LHSGTIGGGLVEAKALEFAQDLLAETNPAEQTKFVEWNLNRDAGMSCGGSVKLFFERF
                     HANPWEIIVFGAGHVAQALIPLLLTLECKLTCLDTRNEWLNKLPETPKLKKLCVESLP
                     ESVEETSEKAFVLIMTQGHRSDFYVAQRFLARKELPFLGVIGSRSKAATLKRELKKEG
                     LSEEQTKRLVCPIGFSLGGNHPQEIAISITAQLLFERDKLFGKIHPRNPVPEKP"
     gene            7076..7672
                     /locus_tag="EYO68_04645"
     CDS             7076..7672
                     /locus_tag="EYO68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07129.1"
                     /translation="MNQLNISLPNWIDSFLGEYPKTIPDPEKQMRFVLALTERNIREE
                     TGGPFGAAVFERDSGKLVSVGVNVVVRQNCSAAHAEMMALMLAQKELGHFDLGADGFP
                     GHRLVTSGKMCAMCLGNVCWSGVSEVLSSAEPEDVEKIAGFDEGPTPPDYNAQLERRG
                     IKIVPELLRSEGRAVLQLYVDLGGKVYNARHSQESSLT"
     gene            complement(7658..8344)
                     /locus_tag="EYO68_04650"
     CDS             complement(7658..8344)
                     /locus_tag="EYO68_04650"
                     /inference="COORDINATES: protein motif:HMM:PF03466.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIC07130.1"
                     /translation="MELYAVTQRMEILEREAQDVLQTAGGFQSGHLRVGAVGPYHLTE
                     MLAVFRERFPYLKITVTVRNSEDILENLLNFEVDVAVLSQTETDSRILAVPFCQEPLV
                     VFVHSGHPWASRETLRIEELEGQDVILREPGSTTRRAFEKALKQTGVTIRPVMEIGSR
                     EAVWMAVARGLGIGVVSNLEFMPHPNLRKLSFENADVHTNAHVVCLRERRDSRMIHEF
                     FQIVEELRQT"
BASE COUNT         2470 a         1746 c         1963 g         2186 t
ORIGIN      
        1 ctgataatgc tcaacaaagc gctctataac gtcgttaaaa gctcccggat ctgcaagcag
       61 ggttgtgatg tcccggaata gaattccggg tgccggaaag tcgggaatcg tacggatttt
      121 ctgttttaag tcattcatgc ggtttgcata aaacgaaaaa aatggttttg aagggcttca
      181 gcaaattcta actgcggaag actgattaca tggatcgtga aatcgtgatt gttaccaggg
      241 acaattattt tatcatacac acgtttgaaa acgaactaaa aataacactc cggcccttga
      301 agacaataac gtttattctg aaatattcct tggataaact gagtttgact tgcaagaatg
      361 attaaaatct aatctaatta gaggttgatt catactacta ataagctgac attaaaaact
      421 aaaataaaaa caggactcgg atgattcagg aattaagaga atattcaaat aacttatttt
      481 tcaaacttct catgggcgtt attgccatca cttttgtctt atcgtttgga gtaggaggtt
      541 tttttggtga ccgcaaggaa gttgtggcca tagtgaacga tcaggaaatt ttactcaagg
      601 aataccgtga aacttatcaa aaccggatgc gtgctttcca ggaacagttt ggagaaaatg
      661 cagaaaaatt tgcagaacag ctgaatctgc gtcaacaagt ttttaatcag ctcattgacc
      721 ggcacctgct cctgacggat gcagctgaac tcaacctgtt ggcaacagat ttggaacttc
      781 aggacttcat ccgccggcag gcgtttttcc aaaaaaatgg acagtttgat tatgatactt
      841 atgaaactgt tttaagtcaa aacaggattg tgcgacatga atacgaaggt tctctgaggg
      901 cagacctttt acttgcaaaa aaacaacagc tgcttggtac aggtctggtg atcagttcaa
      961 gagaggtcga acaagcttac cgcatggact ttgaggaaat tgaagtggag tatgtgtttt
     1021 ttgatccaca gattttcatc gacaaaacaa cagtaaatca agtggatctt agaaggtacc
     1081 atcgggagca tccggatgaa ttccagactc tgaatcaatt caaaattgag ttttatactc
     1141 tttcagcaga ttactacaaa gatattgtca aggttcgtga acgtgaagta cgccgttact
     1201 tcaagaaaaa tactgagagt tatgttactc caccgcaaat aaaggctcgt cacattcttt
     1261 tgaaacttcc tccggattct ccgaaagaaa cacttactga aaaacagcag cagttggaaa
     1321 aactgctgac gcaaatcaag tctggaacgt catttgagga gttggcaaga gagcattctg
     1381 aagatggaac agccgcagat ggcggtgatt tgggctggtt taagcccgga gaaatggtgc
     1441 cggcttttga aaccgctgct tttgccctgg ctgcaggaga agtgagtgat attgtacagt
     1501 ctccatttgg gttgcacctg attaaagtcg atgaacttaa agaggaaatt acaaaatctt
     1561 tggacgaagc acgagatgaa ataacgggta ttcttgcaga aagccgagca caaaaacgac
     1621 tcgatgaaga cctggacaga ctttcaggac ttgccggaga gtcatttact gaggaagccc
     1681 ggaaattaag caaagaggtg caaatctcaa agtggtttga caaatctcag gtcatacccg
     1741 gacttggttc cgcttcagga cttgttacag agttactgag tcggaaacct ggagaaatgg
     1801 gtgtctggag acgtaatcct attttgggac atgtggtcta ccgtctcagt caagcaaaag
     1861 aaccggtcac acgtgacttt gaagaggcaa aggaggatgt cgaaactgca gtacgtcttg
     1921 aaaaagccag atcactggcc cttgaaacag caaaaaccgc tttgactcaa gttaagggag
     1981 gtgagaacat tgaaagactt gcagataaac ttgggctaaa aacagaaact ttggccttta
     2041 ccgcaaacac tagattcctg cctggagtgg gtgacaatac agaattccgt aaagtgggct
     2101 tgcatttaaa tcaaaatcaa cttttcggct taagcttgaa tgaaaatcgt gcggacctga
     2161 ttcactttaa aaaacgtact cttgcagtag aaaatgcaga tgagcagaaa gacaaagtca
     2221 gggcacagct ccgtcaaaat ttgcagcagg cactgctcag taaggaactg aagcgcttgc
     2281 gggattctgc atcgattgag gtcattaatc cggtgttccg cacacctggt gcctgaggtt
     2341 taattctctc caatttgatt tttgtttact gttcattgat catagcagct tgatcattgt
     2401 tcatcggtat ctgatcattg aaaaatgcct ctccttcact attcaaaccg cctggaatgt
     2461 ctaattgtgc ctctggcaca agagctggaa aaacgggatc cttttgattc ggcagagata
     2521 gtggtgccga atttcagcct ggaaaaatgg atctctctca aactcgcaca atttcaggga
     2581 attgccgcaa acctgcgttt tatcacgctg gaaaaggcca tcaacgaggg tctgcaaaaa
     2641 aaattgagcg gccgatttta cgccctgctt aaatcggaaa ccacgcagtg cctgctgctg
     2701 gaagtattac gtaaaaagct aaaaacttca gatcctctct ggttgccggt tcgcagttac
     2761 attacaccaa aaacaaaact aagtccggaa gcaggagaac atcgactata tcagttagcc
     2821 ggacgcttaa cgcatttgtt catggaatat gaactttccc gaaacgacga aattctaact
     2881 cactggctgg ctggacgaaa tgcgtttgat caaggttctc tcggaacaga aagctggcaa
     2941 agggaactct ggagcgaact ttttggtccg gaaggcaaag tgactcgtca taaccgcagt
     3001 gcccgtcaat cagaatcagt agaattccag ccggaacttt atacattacc gcagctttgc
     3061 cggctttata gcagtgaatc aaaacagcaa aattctattc tttattctca atcctcaaat
     3121 aataaactaa aacctctgca tatttttggt gtttcatatc tttccagctt tcatcaaaaa
     3181 gcacttactg aacagctggc gaacctgcgt gatattcatg tctataccct caatccatgt
     3241 atggaatttt gggaggatgt gcagagtttt ggagaatcta aagccgctgc tcggcggtcc
     3301 ctggaagcag agcgtcaacg ttttgaaaaa tatgaggttt tgtccgagac agaaattgaa
     3361 atgggtgagt tgtttcagga tgaagaggat aatcctttct tacaggcctg ggggcgtccc
     3421 ggacgtgaaa atataaaact gctgaatcaa tggagtgctt ggtgttttga accatggttt
     3481 gcggacgcag attcatcggc ggcacagact gaaaactcag agaaacccca gcaaagcgta
     3541 ctcaaccaat tacagcagga tattttattc agggaaccgc gccgtgccga accgcttaat
     3601 ttggagcagg atgaaagttt agttgttctc gcctgcgcaa atccaaggcg tgaagttgaa
     3661 gcagtggcga gcttgatttg ggactggatt cgccgtgatc cagatttgca gctaaatgaa
     3721 tgtgcgctca ttgtgcatga tatggagctt tatcaacatg aaattgaaca agtatttgaa
     3781 agcatttaca atttgcccta ccacttgata gacggaatca gtggaagcgc aagccgtttg
     3841 gaggatgcag caaactccct tttgggttta tgcttcacgg aatacacacg gcgggatatt
     3901 ttcagtttaa tcaacaatcc ctgttttctg cagaaatttg acgatacagt ctcagcagga
     3961 aaaaattcat atggtgagcc gctgcaaatc cagcaatggc ttcaatgggc agatgagcta
     4021 aatgtttttt acggcatcga caggaagtcg caggaggagc agggctacct tcatttagaa
     4081 catgacattt accactggga acaggctttt cagcgtttga cgttgggcga aatggtgtct
     4141 gcagaagaag gaaacggtat cttctcaaat ggcgatcagc agattgtgcc ggttgatcta
     4201 gcagaagaat ggagtgcaga agccgcacgt tttatgttga tagtgcgctc tttaattgcc
     4261 gataccaggg atttgccaaa atggaaaatg tgcggaaaag attgggggcg ctacttgcag
     4321 actctgctca aaacatacct caggccaatt gaagaagcag atgaagaagc attccagaat
     4381 ttgttgagga atgtgcttgc atttcgtgat ttggatttag gagatgagag cggtccacgc
     4441 cagtttagtt tcagcactat tttcgaattt ttcaagcaaa agcagcagtc tgcaactgtg
     4501 catcgcgggc attatttagc agaaggagtc actgtctcat catttaagcc aatgcgtccg
     4561 attcctttca aggcagtttt tttgttaggg atgggcgagg ggttgtttcc aacgccgtac
     4621 cggcgcgaca acctggattt aagacacatt cccgtaaacc tgaatccagc tgtagaggga
     4681 caggccatcc gggagcgtcg attaggtgac gtttcggtga cagagcgtga ccgttacatg
     4741 tttttggaaa cgctggtttc tacgagaaaa catcttgtcc tgagttacgt ttcacgcaac
     4801 gaccgtacag atgatgagct caatccttcc tcaatcattc aaactttaat cgatgaactg
     4861 gacagtggat atttgaaagc aaaatacgag ataatccgac acccgctgaa accgtattct
     4921 ctagagtatt ttccggaact atctgcggaa caaatagact ttgccgacaa tcaaaatcct
     4981 gcatcgaaaa cccattaccc aaattacgat cccgctgcat tttatcaggc acgggcattc
     5041 aggatgaggg agttatttga tcaggagttt cccagttccg gatcttccgg cggatttcag
     5101 cgtatttctc cggactggct ttctcctgaa gttatgctgc ccataacagc aggtgcgttt
     5161 cgtattaaag caacagaatc tgtagcagta aaacagcttt cgtctgttgc attttccagg
     5221 ctgcggaaat ttcttgaatc tccgctgcag tccactgccg gtcatttgct gggccttgct
     5281 gaagatgaag aagatgtaag tgaaaaagtt gatgagcctc ttgttttagt acggctgagc
     5341 gaatggctac tgctccggaa agtctggaac atgggcttga atgtgcctgc aaaaggttca
     5401 cagcagaaat cacatccgga gtggcagaag ctatatcgac ttcaggcgca acgcatggaa
     5461 ctgcagggag aaatgcctac cggcattttc ggagaagcaa tgcagatccg gcacctgcgg
     5521 attcttaaaa actggcaaaa acaactcact gcagtactcg aaactgattg gaacagcctg
     5581 caaaaaaatc ttagccagtt ttattttggt gcagtcagga agggaaaatt taatgaagga
     5641 cttaccggca cagatagaat tttgccggcc gtcaatatcc aaaaagaaaa cccggaaccg
     5701 tctgacagtg aaaatatgta cttcaaaatt caaggagaaa cggaatggtg gtacacagat
     5761 gaatccggta accggtatgt catttacttc agtgaacgag aaagtaaaga caaagcctgg
     5821 ctgcgtcatt tcctggatgt gctggttctt cttgcagtag acataatccc ggaaggcaca
     5881 caagttacgg gattatgcat tacaggtgaa ggaaaacgaa aatccagaaa aattcatctg
     5941 ccttcacgac agcaggcaca agattatctg gctaatttgc tgcaggaaat gaatactgaa
     6001 catgcggctg tgttaatgcc tgtagaatct gtacttgaat tggcaaagga aaacttgaca
     6061 ggtgctgact acaattcaag atatacagaa tggatggacg ccaaacttaa ctcatccagt
     6121 gaaaatcttg gaataagttc ccaatatgga ccagttaaat ttttgacgga catagcatat
     6181 ccggaaaatc ctcatcaatt gatgaaccgc cgttttcaat tgttttttga aacagtattg
     6241 gtttgagtca aataacagat atggaaaatc atctcaaaaa aatgcaggcc ttgctggaag
     6301 aaggaacttc atttgtatct gcaacactgg ttgagattaa aggaagtaca ccacgtgctc
     6361 aaggcggacg gatgctggtt actgcatcag gcctgcattc cggtacaatt ggaggtggac
     6421 tggttgaggc aaaggcattg gaatttgctc aggatttatt agcagaaacc aatcccgcag
     6481 agcaaacaaa atttgttgaa tggaacctca atcgtgatgc aggtatgagt tgcggcggtt
     6541 cagtcaaact cttttttgag cgattccatg ctaatccctg ggaaataatt gtttttgggg
     6601 ccggtcatgt tgctcaggcc ctgataccgc tgctgctgac tctggagtgt aaactgactt
     6661 gccttgacac caggaatgag tggttgaaca aattacctga aactccaaaa ctaaaaaaac
     6721 tttgtgtaga atcccttccg gaaagtgttg aagaaacctc cgaaaaagct ttcgttctga
     6781 taatgacaca gggacacagg agtgattttt acgttgcaca acgatttctt gcacggaaag
     6841 aattgccgtt tttaggagtg atcggcagcc gttccaaggc agctactttg aaacgggaac
     6901 tcaaaaaaga gggcctcagc gaagaacaaa ctaagcgtct tgtttgtcct atcggttttt
     6961 cactcggagg aaatcatcca caggaaattg ccatcagtat caccgcccaa ctgctttttg
     7021 agcgggacaa acttttcgga aaaattcatc cgcgtaaccc agttccagaa aaaccatgaa
     7081 ccaactaaac attagtctac caaactggat tgattcattt ttaggtgagt atccaaaaac
     7141 aattcctgat ccggaaaaac aaatgcgctt cgtcctcgcc ttgacagagc gtaacatacg
     7201 tgaggaaaca ggcggaccat ttggagctgc tgtttttgaa agggattccg ggaagctggt
     7261 atctgttggg gtcaacgtag ttgtccggca aaactgctct gctgcgcatg cagaaatgat
     7321 ggcgctgatg ctggcgcaaa aagagttggg acactttgat ttgggtgcgg acggttttcc
     7381 aggacaccgc ctggtaacaa gtgggaaaat gtgtgcaatg tgcctgggga atgtctgctg
     7441 gtccggagtc agtgaggtgt tgtcatctgc agaaccggaa gacgtggaaa agattgccgg
     7501 ttttgatgaa ggccctaccc ccccggatta taacgcgcag cttgaacgaa ggggaattaa
     7561 aattgtgcct gaattactgc gcagtgaagg ccgagctgtt ttacagcttt atgttgatct
     7621 tggcggtaag gtttataacg caagacacag tcaggaatca agtttgacgt agctcttcca
     7681 caatctgaaa aaattcgtga atcattcggg aatctcgacg ttcacgcaga caaaccacat
     7741 gtgcattagt gtgtacatct gcgttttcaa aacttaattt tcgtaagtta ggatgcggca
     7801 taaactccag attcgagacc actccgattc ccaaccccct ggccacggcc atccagactg
     7861 cttccctgct gccgatttcc attaccggac gaatcgtgac cccagtctgc ttcaatgctt
     7921 tttcaaaagc gcgacgtgtt gtagaaccag gttcacgtaa tataacatcc tgaccttcca
     7981 gctcttcgat ccggagagtc tcccgacttg cccaggggtg tcctgaatgt acaaaaacaa
     8041 ccaggggttc ctgacaaaac ggaactgcta aaattctgga atcggtttct gtttgtgaaa
     8101 gtactgcaac atctacttca aaatttaaca ggttttctaa aatatcttct gaatttcgaa
     8161 ctgtgaccgt gattttcaaa tatggaaacc gttcacgaaa aacagccagc atttccgtca
     8221 aatgataagg tccaacggct ccaaccctga gatgaccgct ctgaaatcct cctgcagtct
     8281 gaagtacatc ctgggcttct ctttccaata tttccatgcg ctgagtgaca gcatagagtt
     8341 ccattcctgt ttcagtcaac tcaac
//
LOCUS       DTVD01000132            8306 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2398_length_8306_cov_234.639559, whole genome
            shotgun sequence.
ACCESSION   DTVD01000132 DTVD01000000
VERSION     DTVD01000132.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2398_length_8306_cov_234.639559"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="EYO68_04655"
     CDS             complement(<1..1362)
                     /locus_tag="EYO68_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC07131.1"
                     /translation="MGDLIGKSVKRVEDNRFLKGEGKYTDDFNMPNQTFAVYVRSPHA
                     HANLVSVDISAAKAMDGVINVFTGKDIKDAGIVGSICGWQVDFKNGDTMKEPGHPILA
                     VDKVRHVGDAVALVIAEDIGKAKDAAGAVEVNYKVLDAIVDPKAAAQDGAPQVHDDVP
                     NNTIFDWELGNKDETDAAFENAHHITELSYHNQKLVPNAIEPRAALAYFDSNDEKYTL
                     YTTSQNPHLARLIIAAFVLSIPEHKLRVIAPDVGGGFGSKIFTYNDECGVLWASKQIG
                     RPVKWTADRTEAFFTDCHGRDHVTDAKLALDADGNITGLRTKTYASLGAYLSNFSTCI
                     PTYLHGTLMQGLYTTPAVHVDVTAVVTNTVPVDAVRGAGRPEATYGIERLVETAAREI
                     GMDPAEFRLKNFIPPFDGVKQPGYQTQVALQYDSGNYEGALKKALKNANYEKLKKERA
                     AARA"
     gene            complement(1371..1829)
                     /locus_tag="EYO68_04660"
     CDS             complement(1371..1829)
                     /locus_tag="EYO68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC07132.1"
                     /translation="MKVSMKVNGTAYEHDVEPRTLLVQHLRENMGLTGTHVGCDTSSC
                     GACTIIVNGETVKSCTLLAVQCDGAEIKTIEGMATNGELHPIQESFREKHGLQCGFCT
                     PGMVMSSWQLLERNPKPSEDEIRHAIEGNFCRCTGYHNIVKAVQDAATKI"
     gene            complement(2073..3689)
                     /locus_tag="EYO68_04665"
     CDS             complement(2073..3689)
                     /locus_tag="EYO68_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIC07133.1"
                     /translation="MKEAYESLRKNPANHVPLTPLSFLHRTADIYGEREAIIYGERRY
                     SWRQLRERCLCMASSLTELGVGLGDTVAVLAFNTPELFEAHFSVPMTGAVLNTINTRL
                     DAETVAYILKFGQVKALIVDRELLPLAQKALQDEQIKLKIILIDDDSAEVKPSVELEV
                     LEYEELINSGDPQFTCPPLQDEWQALSLNYTSGTTGRPKGVVYHHRGSYLMSMGTVTA
                     WELPHHPRYLYCVPMFHCNGWGHAWTMTLVAATVVCMRSFSPKLLFDLLERHDITHFG
                     GAPVVLNMLANAPAEEQKHFDRSIKVMTAGAPPPAKVLESMTEFGFDVLHVYGLTETY
                     GHILESAPQSDWQDQSAARRSELISRQGVRFPIMEEVSVIDPKTKKVVPSDGKTIGEI
                     VMRGNTTMKGYLNNKEATAEAFDGGWFHSGDLAVVQEDGYIQIKDRAKDIIISGGENI
                     SSVEIENVLYKHPAVGEAAVVAMPDDKWGEVPCAFVELKAGESTKDQELIEFCTQHMA
                     RFKRPKKIIFGPLPKTATGKIQKHELRQRI"
     gene            complement(3789..4922)
                     /locus_tag="EYO68_04670"
     CDS             complement(3789..4922)
                     /locus_tag="EYO68_04670"
                     /inference="COORDINATES: protein motif:HMM:PRK12486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoniopropionate demethylase"
                     /protein_id="HIC07134.1"
                     /translation="MRPELLISSRTRSTPFSSRVEACGVKAYSVYNHMLLPLIFRSLE
                     EDYWHLCESVQVWDVSCQRQVEITGPDTQKLVQLMTPRDLSQAELGQCFYAPLCDETG
                     GMINDPILIKHSNNHWWLSIADSDVMLWAKGLATGFGLDALVTEPDIWPLAVQGPKAE
                     ELLSRVFGKEISRILFFRSRTFNYQGTKMLVARSGWSKQGGFEIYVNDAELGGQLWDE
                     LFAKGEDLNVGPGCPNLIERIESGLLSFGGDMGYDTTPFECGLDQYVSLDADIESLSL
                     EELRTRKSKTKLIGIVIDRKVNLIDKRVFIGSNVVGKITSDTWSPRYSAFLAFARCEL
                     KNLEDAQKLYVKTTSGEVSARITDLPFDFETLGLTGIEEGTSI"
     gene            complement(4926..5957)
                     /locus_tag="EYO68_04675"
     CDS             complement(4926..5957)
                     /locus_tag="EYO68_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HIC07135.1"
                     /translation="MPKIELKNVYKIFGEDPNSVLPLVQKGATKEEILEDTYHTVGLD
                     NVSLSIEEGETFVCMGLSGSGKSTLIRHINRLIDPTEGEVLVDGVNVLEFNEKELLEL
                     RRHEMSMVFQRFGLFPHKTVMENVAYGLEVQGKPMEDRSHIAGEQIKAVGLNGFEHQY
                     PSQLSGGMQQRVGLARALATNPGILLMDEAFSALDPLIRNDMQDQLLELQSKLHKTIV
                     FITHDLDESLKLGDHIGILNGGKLVQVGKPEEIIMNPADDYVAAFVKDVNRAKVLRAK
                     TIMLSPEKYTANGKGSGETHKVLENSFIEEFLPLVLEKRSVVEVIDKDGNTMGFITEK
                     ELGQSLSKT"
     gene            complement(5957..6979)
                     /locus_tag="EYO68_04680"
     CDS             complement(5957..6979)
                     /locus_tag="EYO68_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HIC07136.1"
                     /translation="MKTNWKYWVPICFVAFWGLIIISYRYAKPRYKPENISDLLDDFS
                     WLINWPKWLDFPLMQPLNAGFDWLITEYGMFFDGINNLLLGLYTTMKDAVVGLPWPLV
                     VVLVIVMTYFVSGKKMGTTIMVGFCTFFIGFLSPRYWDKAIMTTCIVLIGIFLCLVIG
                     IPIGIAMARKPKFRKTLLPLLDLMQTIPSFCYLIPGILLFGLGAVPAIIAIFVYAAPP
                     LIRLTDLGIRLVDKEVVEAADAFGASKKQKLWGVQIPLALPNIMQGVNQSVMMALAMV
                     VIASMIGTRGIGDEVLLGLQQLNVGMATEAGIAIVLLAIIFDRITQSYGDKIQERTMP
                     KKLKKV"
     gene            complement(7058..8083)
                     /locus_tag="EYO68_04685"
     CDS             complement(7058..8083)
                     /locus_tag="EYO68_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC07137.1"
                     /translation="MYTIKKVLLSLSLLGTVLVFSTSAYAACKVHLGDFDWDSANVHT
                     AISQFILEKGYGCKVRVSKGSTTPIMAALFDQQIDIITEVWRDNLVQLIEDNLAKGNI
                     IELGINTPESGQGFYVDKPTSDKYGLKHVNDMKSAKIAALFSDPEAPGKGRMTSCISG
                     WTCYTINFVKHKVYGLDKYYTNFDPGSGGALDAAIAGGFKKGKPVFSYYWEPTGLMGK
                     VDLVKLKEPTYEQGCWDRMTTVVEDIKKNGPDVYKPTCACAYVDMALTKAASTKFANN
                     PANKPILDFIKAYSIPTALVNSSLAFYMDESGGDMEETAKNFLSNSNIWESWVPSDVA
                     SKVKAAL"
BASE COUNT         2196 a         1975 c         1698 g         2437 t
ORIGIN      
        1 agcacgagcg gcagcccttt cttttttgag cttttcatag ttagcatttt ttagagcttt
       61 cttcaacgcg ccttcgtagt ttccgctatc gtattgcaaa gcaacctgag tctggtagcc
      121 cggttgtttt acaccatcaa atggcggaat gaaatttttg agtcgaaatt cagctggatc
      181 catgccaatt tcccgtgccg ctgtttctac aagtcgctca atgccatatg ttgcttccgg
      241 gcgaccggct ccacgaacag catccactgg gacagtattg gttaccactg cagtcacatc
      301 cacatggacg gcaggtgtag tatataatcc ctgcatcaaa gttccatgga ggtaagtggg
      361 gatacacgtt gagaaattgg acaaatatgc tccgagagat gcataggtct tggttcgcaa
      421 tccggtaata tttccatcag catctaatgc caatttcgca tccgttacat gatcccgacc
      481 atggcaatct gtaaaaaatg cttcagtccg gtcagctgtc cattttacag ggcgaccaat
      541 ttgcttggat gcccaaagaa caccacactc atcattgtaa gtaaatatct tacttccaaa
      601 tccacctccc acatccggtg caatgaccct cagtttatgc tctggaatac tcaatacaaa
      661 tgcagcaata atcaatcgtg ccaagtgtgg attctgtgaa gttgtgtata gggtgtattt
      721 ctcatcatta ctgtcaaagt acgccagtgc agcccgtggc tcaattgcat tgggaaccag
      781 tttttggtta tggtaggaaa gttcagtaat atggtgtgca ttttcaaacg cagcatctgt
      841 ttcatcctta ttcccgagtt cccagtcaaa gatggtattg tttgggacat catcatgaac
      901 ctggggtgca ccatcctggg ctgctgcttt tggatctaca attgcgtcca aaactttata
      961 attgacttcg actgctccgg cggcatcctt ggctttgcct atgtcttcag ctattaccaa
     1021 agccacggca tcacccacat gtcttacctt atcaactgcc agaataggat ggccaggctc
     1081 tttcattgta tcgccgttct taaaatcaac ctgccatcca caaatagacc ctactattcc
     1141 agcgtcttta atgtcttttc ctgtaaaaac gtttattaca ccatccattg ctttagcagc
     1201 agaaatatca acgctgacta aatttgcgtg tgcatgcgga cttcgcacat aaactgcgaa
     1261 ggtttggttt ggcatattaa agtcatcggt gtatttacct tcacctttta gaaatcgatt
     1321 atcctctact cgtttaactg attttccaat taagtcgccc attgagtctc ctaaattttt
     1381 gtggcggcat cttgaaccgc tttgacaatg ttgtggtaac cggtacagcg acagaagttt
     1441 ccttctatag catgccttat ttcatcttct gatggttttg gattgcgttc cagcaattgc
     1501 caagaagaca taaccatacc gggagtgcag aatccgcact gcaacccgtg cttttcccgg
     1561 aaactttcct gaataggatg cagttctccg tttgttgcca taccttctat agtctttatt
     1621 tccgcaccat cacattgcac agcaagcaaa gtgcaggatt ttactgtctc accgtttacg
     1681 atgattgtgc aggctccgca actgctggta tcacatccaa catgtgttcc ggtcagcccc
     1741 atattctcac gcaaatgctg cacaagaagt gttcgtggtt ccacatcatg ctcgtatgcg
     1801 gtaccgttta ctttcattga aactttcatc gatctgttcc tctaaagaaa gttaattaat
     1861 cagccatgaa aggcccgtga aatcactttt ttatcgatta ttaaagctat aaataacacc
     1921 gatattattt tttgtcaaat tatttttcaa tttttttcaa attaactaaa aataattaag
     1981 gacacacaat acttaattca aaaaagacat gtcatgtttc taaaattcgt tgtaatttac
     2041 tgttgaaaat gccttttgaa ctagatcgtt gattatattc gttgacgcag ttcatgtttt
     2101 tgaatttttc cagtcgcggt tttgggcagg gggccgaaaa ttattttttt tggtcttttg
     2161 aaacgggcca tgtgttgtgt acagaattcg ataagttcct gatcctttgt actttctccg
     2221 gcttttaact caacgaaggc acagggtact tctccccatt tatcatcagg cattgcaaca
     2281 actgctgctt ctcctactgc aggatgctta taaagtacgt tttcaatctc aacagaggaa
     2341 atattctcac ctccggaaat aataatatcc tttgctctgt ctttgatttg aatgtagccg
     2401 tcttcctgta caacagccag gtcaccacta tgaaaccaac cgccgtcaaa tgcttcagca
     2461 gttgcttctt tgttgttcag atagcccttc attgtggtat taccgcgcat cactatttcg
     2521 ccgattgttt taccgtcgga tggtaccact tttttagttt tagggtcaat cacagagact
     2581 tcttccatta ttggaaatcg aacaccctgg cgagaaataa gctctgatct gcgagcggca
     2641 ctttgatcct gccaatctga ctgaggtgct gactccagaa tatgaccata cgtttcagtt
     2701 aaaccatata catgcaatac gtcaaaacca aactctgtca tactttccag aacctttgcc
     2761 ggcggaggag cgccggcagt catgactttt attgagcgat cgaaatgctt ttgttcctca
     2821 gccggggcat tggccagcat attcagaacg acaggagcgc cgccaaagtg cgttatatca
     2881 tgacgttcca gcaaatcaaa aagcagcttt ggactgaatg agcgcatgca aaccactgtt
     2941 gcagccacta atgtcatggt ccaggcgtgg ccccaaccat tgcagtgaaa cataggtaca
     3001 cagtaaagat aacgcgggtg atggggtaat tcccaagcag ttactgttcc catactcatc
     3061 aggtatgatc cacggtggtg atatactacg ccctttggac ggcccgtggt tccggaagtg
     3121 tagttcaatg aaagtgcctg ccattcgtcc tgtaaaggcg ggcatgtaaa ttgagggtct
     3181 ccggaattaa ttaactcttc atattccaaa acttctaatt cgactgacgg cttaacttct
     3241 gctgaatcgt cgtcaatcag aattattttt agtttgatct gttcatcttg aagagctttt
     3301 tgagccagtg gcagcaactc acgatccaca atcagtgcct tgacttgtcc gaatttcagg
     3361 atatatgcaa ccgtttcagc atctagtctg gtgttgatag tgttgagcac ggcaccggtc
     3421 atgggtacgg aaaagtgtgc ttcaaacaat tccggtgtat tgaaagccag tacagctact
     3481 gtatctccca aacctacacc aagttctgtc agactggagg ccatgcacag acagcgctcc
     3541 cgtaactggc gccagctgta tcgtctttca ccgtatatta ttgcctcccg ctcaccataa
     3601 atgtctgcag ttcggtgtag aaatgagagt ggagtcagag gaacgtggtt agccggattc
     3661 tttctgagcg actcatatgc ttctttcata attcaaactc actttctttg aaatgacgaa
     3721 acaaagttcg gttctatttt taagaatttc tcaaaaatgc aaatgtaaat ctatcgcaaa
     3781 agttaaggtc aaatactcgt accttcttca atacctgtca atccaagagt ttcaaaatca
     3841 aagggtaagt cggtgatcct ggctgacact tctccagaag tagttttaac atataatttt
     3901 tgagcgtctt ccaggttttt gagttcacac ctcgcgaatg ccaggaaggc tgaatatcgg
     3961 ggagaccatg tgtcggaagt aatttttcca accacattac tgccgataaa tactcttttg
     4021 tctatcagat tgaccttacg atcaatcact attccaatga gcttggtttt tgactttcgt
     4081 gttcttagtt cctcaagact caggctttca atgtctgcgt ccaaactgac atactgatca
     4141 agaccgcatt caaacggcgt tgtatcgtat cccatatctc caccaaaaga cagcagcccg
     4201 ctttcaattc gctcaatcaa gttaggacag cccggaccaa cattcaggtc ttcacctttt
     4261 gcaaaaagtt catcccagag ttgtcctccc aactccgcgt cattaacata aatttcgaaa
     4321 ccaccctgtt tgctccaacc ggatcttgcc accaacatct ttgttccctg gtagttaaat
     4381 gtcctgctgc ggaaaaaaag gattctgctg atttcttttc caaaaacccg ggaaagtagt
     4441 tcttcggctt ttggtccctg aactgcaagc ggccaaatat caggttctgt aacaagggca
     4501 tccaatccaa aaccagttgc cagccctttg gcccagagca ttacatctga atcagcgatt
     4561 gaaagccacc agtggttgtt gctgtgtttg atgagaatgg gatcattgat cattcctccg
     4621 gtttcgtcac agagcggagc ataaaaacac tgtccaagct ctgcctgtga caggtcacgg
     4681 ggagtcatta actggacaag tttttgtgtg tccggtccgg tgatctcaac ctgtctctgg
     4741 caggaaacat cccagacctg tacggattca cataagtgcc agtaatcctc ttccaggctg
     4801 cggaagatca aaggcaggag catgtggtta taaactgaat atgcttttac accacaggcc
     4861 tcaacccggc tgctgaaagg agttgaacgg gttctggagg aaattaacag ttcaggacgc
     4921 acagttcagg ttttggagag tgactgtccc agttcttttt cagtaatgaa tcccatagtg
     4981 ttaccatcct tatcgatgac ttccaccacg gacctctttt caagtaccag tggcaaaaat
     5041 tcctcaatga agctgttttc cagcaccttg tgggtttctc ccgatccttt gccgttagcg
     5101 gtatattttt cgggactcag cataatggtt ttggcgcgca gtaccttggc cctgttgaca
     5161 tccttaacaa aggccgcaac gtaatcatca gccggattca tgataatttc ttcaggcttt
     5221 ccgacctgta ccagtttacc gccgttgaga atacctatgt gatcaccaag tttcagtgat
     5281 tcatccaggt cgtgggtaat gaagacgatg gtcttgtgca gtttagactg tagttccagc
     5341 aactgatcct gcatatcgtt gcggatcagt ggatccagtg cgctgaaagc ttcgtccatc
     5401 agcaatattc cggggttggt cgccagtgcc cgtgctaatc cgacacgttg ctgcatgcct
     5461 ccggacaact ggctggggta ttggtgttcg aatccgttta gtccaactgc cttgatctgc
     5521 tctcccgcaa tgtggcttcg gtcttccatc ggcttgccct gaacttccaa accgtaggcg
     5581 acattttcca tcacggtctt gtggggaaaa agcccaaagc gctggaagac catgctcatc
     5641 tcgtggcgcc gtaattcgag aagttccttt tcgttgaact ctaaaacgtt aacgccatca
     5701 accaggacct ctccctctgt cggatcaatc aagcggttga tatgtcggat cagcgttgat
     5761 tttcccgaac cggacaatcc catacagacg aaggtttctc cttcttcgat actgagagaa
     5821 acattatcaa gccccacggt atgataggta tcttccagta tttcttcttt tgtagctcct
     5881 ttttgcacca aaggcaaaac agagtttggg tcttctccaa agattttgta gacgttctta
     5941 agctcaatct tgggcattat actttcttaa gtttcttggg catggtcctc tcctggattt
     6001 tatcaccata ggactgtgtt atacgatcaa aaataattgc cagcaaaact attgctattc
     6061 cagcttctgt agccattccc acgttgagct gttgtaaacc aagcagaact tcgtctccaa
     6121 ttccacgagt accaatcatc gaggcaatta caaccattgc taatgccatc atcacagact
     6181 ggttgactcc ctgcatgatg tttggaaggg caagaggtat ttgtactccc caaagtttct
     6241 gttttttgct ggccccaaaa gcatctgctg cttcaacaac ttctttgtct accagccgga
     6301 ttccgaggtc agttaacctg atcagcggag gagcggcata aacaaaaatt gcgataatag
     6361 caggtactgc tcccagccca aacaacaata tacccggaat caaataacag aaacttggga
     6421 ttgtttgcat caggtccagt aacggcaaca atgttttcct gaattttggt ttcctcgcca
     6481 ttgctattcc gattggaatt ccaattacca ggcagagaaa aattccgatg aggacaatac
     6541 aggtcgtcat aatggccttg tcccagtatc ttggactgag gaaaccaatg aaaaatgtac
     6601 aaaatccaac catgatggtt gtacccattt ttttgccgct cacaaaataa gtcattacga
     6661 tgaccagtac caccacaagc ggccatggta ggcctaccac tgcatccttc attgtggtat
     6721 aaagacccag cagtaagttg ttaataccat caaaaaacat cccgtactct gttatcagcc
     6781 agtcaaaacc tgcattcaga ggttgcatca agggaaaatc gagccatttg ggccagttta
     6841 tcagccagga aaaatcgtct aataaatccg aaatattttc cggtttatac cggggttttg
     6901 cataacgata gctgataatt atcagtcccc agaaagctac gaaacaaata gggacccagt
     6961 atttccagtt agttttcatt agtcctgtat gcgaagaact aagagcccga acgggctctt
     7021 agttacaaaa agagagattg agtaattaca aagcagatta cagtgctgct ttgactttac
     7081 ttgccacgtc tgagggaacc caactttccc agatgttgga gttagagagg aaatttttgg
     7141 ctgtttcttc catatctcca cctgattcat ccatgtaaaa agccagcgaa ctgttcacca
     7201 gagccgttgg aatagagtag gctttgataa aatctaaaat cggcttgttg gcaggattgt
     7261 tggcaaattt agttgatgca gccttggtaa gagccatatc tacgtatgcg caggcgcaag
     7321 ttggtttgta gacatccggg ccgtttttct tgatatcttc aacaactgtt gtcatcctgt
     7381 cccagcatcc ctgttcgtag gtcggttctt tgagcttgac caagtctact ttacccataa
     7441 ggccggttgg ttcccagtaa taggaaaaga ccggtttacc tttcttgaat ccaccggcaa
     7501 tcgccgcgtc aagtgcacct cctgagcccg gatcaaagtt tgtatagtat ttatccaaac
     7561 cgtaaacctt gtgcttcaca aagttgatcg tgtagcacgt ccaacctgaa atgcagctcg
     7621 tcatcctgcc tttcccgggt gcttcgggat cagaaaatag agcggcgatc ttagcactct
     7681 tcatatcatt gacgtgtttc aggccgtact tatctgatgt cggcttgtcc acatagaatc
     7741 cctgaccgga ttctggtgta ttaatcccga gttcaatgat attgcctttg gcaagattat
     7801 cttctatcag ttgtacaaga ttatccctcc agacttccgt tattatatct atctgttgat
     7861 caaacaatgc ggccatgatc ggcgtggtac tcccctttga tactcttact ttacagccat
     7921 atcctttttc aagaatgaac tgagatattg ctgtgtgaac attggcgctg tcccagtcaa
     7981 agtcacccag gtggacttta catgcagcat aggctgatgt actgaaaacc aacacggttc
     8041 ccagcaggct caacgacaag agcacttttt taattgtata catcatgttc ctttcattgg
     8101 aaattaagga attgaactaa ttatcatcta atacatgtta aaaacaaatg tgcttagacg
     8161 aaaccaaaaa aacggagctt aatctacact gattatactc cccggttaat tgccggatga
     8221 ggcaacaaac ctgaaacgag cctcacttta acacaaaaat aagtgaaagc aaggttattt
     8281 ttgataatcc aaaaaaaaat gatggg
//
LOCUS       DTVD01000133            8303 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2402_length_8303_cov_342.201261, whole genome
            shotgun sequence.
ACCESSION   DTVD01000133 DTVD01000000
VERSION     DTVD01000133.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2402_length_8303_cov_342.201261"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2183
                     /gene="glgB"
                     /locus_tag="EYO68_04690"
     CDS             <1..2183
                     /gene="glgB"
                     /locus_tag="EYO68_04690"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIC07138.1"
                     /translation="DFHEPRSVLGFHEVRQRNKQQVWIVRVLEPDAEKVSLFWENETE
                     AEATSLKRIHPEGLFELKIPPRPELKPYRLKISYKNGNEHIRFDPYYFSTQLTEYDQF
                     LFAEGNHHRFYHKLGAHPLEIDGVPGTLFAVWAPNAKRVSIVGDFNYWDGRKHPMQIL
                     GDSGIWELFIPEVAAGMLYKYEIKTGQNEILLKSDPVAFTAEQRPSTASVVTELNGFR
                     WQDQKWLEERENRDPLRQPINVYEVHLGSWQRVPEEDNRSLTYPELADRLIPYAVEMG
                     YTHLELMPVAEHPLDGSWGYQVTGYFAPTSRFGTPHEFMEFVDRCHTSGLGVILDWVP
                     GHFPKDSHGLAGFDGTALYEHEDPLQGEHKEWGTLIFNYERNEVRNFLISNALFWFDE
                     YHIDGIRVDAVAAMLYLDYNRKEGQWVKNRHGGRENIGAIEFLKQLHETLFHYHPGIL
                     SIAEESTAWGGVSHPTYAGGLGFNLKWNMGWMNDTLRYIEKDPVHRKYDHHLITFSLM
                     YAFTENFVLALSHDEVVHGKRSMLGKMPGDDWQNFAGLRMYLAFQMAHPGKQLTFMGT
                     EFGQRDEWSENRSLDWHLTQFPDHQKLQEYVRTLNHFYLSKPTLFTNDFDWTGFQWID
                     HHDQDNSVLSFIRRSADEVSDPDLVFIFNFTPVPRDDYLTGFPESGHYSKLLDTDDPK
                     FGGSGYNQRQQIITSPQMWQGQPHRATVNLPPLGCLVFEKMAGM"
     gene            2239..4749
                     /locus_tag="EYO68_04695"
     CDS             2239..4749
                     /locus_tag="EYO68_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIC07139.1"
                     /translation="MPQTRKEMENIWELVATYRSMDRDGLIESIANHLEFSQCKNRFT
                     AEDFDVYRSFALSVRDRLVEFWNDTQQTYLKKKCKQVNYFSLEYLIGRSLNNNLLNLG
                     ISQAGEDAIRKIGYDLEELQELEHDAGLGNGGLGRLAACFMESMATLQLPAHGYGIRY
                     EFGIFKQQFENGAQVEAPDNWLEEGYPWEIPRWEVVYPVHFFGRVKKYTNEKGKLSRK
                     WVETEEVLAMAYDVPIAGFGNHTVNNLRLWSAKPSKSFDFQLFNSGDYIQAVEETQRS
                     GTISKVLYPNDQGFSGKELRLKQQYFFVAASLQDIILRFKVHSETFDKFPEHVSIQLN
                     DTHPSIAIPELMRIFVDEEGLEWNEAWAITTRVFAYTNHTVLPEALERWSVDLMGRLL
                     PRHLEIIYEINDLFLESVKEKYPDDADLLQRISFIEENDHKQIRMPYLSIVGSHTING
                     VAELHTELLKTTVFKDFYKLFPERFQNKTNGITPRLWLRNTNPELSELISEKIGDGWI
                     TDLQKLRKLEPFADDPEFQEAWRSVKRIKKEQLANWLKQTSGVIIDPESLFDVQIKRI
                     HEYKRQLLNILHVTYLYNKILEHPELPFVPRTILLAGKAAPGYYMAKLIINLANDVAR
                     VINSDPAVQGRLNLVFVPNYNVSVAEKIIPATDISQHISTAGTEASGTGNMKFILNGA
                     LIQGTMDGANIEIAEEVGRENIFTFGLSSDEVSILAESGYNPRVSYQNNPGLIEALSM
                     INTGYFNRDKPHLYNDLYNSLVFEDKYMLLEDFASYDECRQKVMNTWKDQNTWTRMSI
                     LNTAGSGKFSSDRTIAEYAKDIWGLEAVTVELQGKKVR"
     gene            complement(4818..>8303)
                     /locus_tag="EYO68_04700"
     CDS             complement(4818..>8303)
                     /locus_tag="EYO68_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIC07140.1"
                     /translation="EKRSVLLEDKYTLREGYAFMTGIQALVRLPLVQRELDLKAGHNT
                     AGYISGYRGSPLGGYDQQLARNKKLLDEHYVKFQPGVNEDLAATALWGTQQAELSGEG
                     KYDGVFGIWYGKGPGVDRSGDVLRHANYAGTSKFGGVLALMGDDHTAESSTTCNQSEF
                     AMLDAMIPVLNPSSVQEIIDFGIIGWMLSRYSGCWVGLKCVHDTVEATSTINVDVARL
                     KLELPNDFNFPVDGLNIRFPDTPQAQERRMHEFKVDAVRSFCRANKLDRTIWPGGNAK
                     IGIVTVGKSYLDTRLAFDELGIDETGAEKFGVRLYKVAMPWPLEPQGILEFAKGLDLI
                     IVVEEKRALIEPQIKEFLYDLSNRPKVIGKTDETGEPLFRTAGALDPNHIAATIARRI
                     LTFRTDEKLKKRLQEMEIRMDSSLLPQESMVRLPYFCSGCPHNTSTVVPEGSIAMAGI
                     GCHYMVQWMDRDTIGFTHMGAEGANWVGQATFSTRKHIFQNIGDGTYFHSGTMAIRAA
                     VSSGINITYKILFNDAVAMTGGQGFDGPMTVQSIIQQMYAEGAKRVDVVSDEPEKFTQ
                     SSGIPANVKVYDRKDLDILQRELREIEGVTVLIYEQVCAAEKRRRRKRGLIPDPPRRI
                     YINDDVCEGCGDCGLKSNCVAVLPLETQFGRKRVIDQSACNKDYSCVNGLCPSFVSVI
                     GGKMRKNDPSANMDVEWTSLPEPKLPVIKGTYNIVLTGVGGTGIVTIGALLGMAAHLE
                     KKGIGILDMIGLAQKGGAVLSHLRIGKSPEDIHSPRIASQGADLVIGGDLVVTGGHKT
                     LSVIKSGHTKLVINSYEMITGDFTKNADMLFPSLEIKQAIQQTAGTDNTEFLDASRLA
                     TALIGDTIATNMFMLGFAFQRGLIPLERSSIEQAIEINGMSVESNKQSFLWGRRTAHD
                     GKRVRELTASIVEGFLLEDPTEGLDELIQHRADVLTAYQNKAYAKRYLQLVERVRTIE
                     TDRLPGSLSLTEAVARYYFKLLAYKDEYEVARLYTNGDFLKKIRSRFEGDFRLKLHLA
                     PPLFSHRDSHTGEPIKSAFGSWIFPVLKMLSRFKFLRGTAFDLFGKTKERRMERQLIQ
                     EYEQTIKELLRGLTKKNHNIALEIAKIPEQIRGYDMVKQRHFETAKSTEKKLLTQFRD
                     SAKITVGLKSSEPVS"
BASE COUNT         2391 a         1830 c         1737 g         2345 t
ORIGIN      
        1 gtgatttcca tgaaccacgc tccgttctcg gctttcacga agtccgtcag agaaataagc
       61 agcaagtttg gattgtacgt gttcttgaac cggatgcaga aaaggtttcg cttttctggg
      121 aaaacgagac ggaagctgag gctacctcat taaaacgaat tcacccagaa ggtttatttg
      181 aactgaaaat acctccgcgt ccggaactaa agccttatcg tttgaaaatt tcctacaaaa
      241 acggaaatga acatattcgg ttcgatccat attatttctc tacacaactt acagaatacg
      301 atcaatttct ctttgctgag gggaatcacc atagatttta ccacaaactt ggtgcacatc
      361 ccttagaaat tgatggtgtt ccaggtacac tgttcgctgt ctgggcacca aacgccaagc
      421 gtgtgagtat tgttggagat ttcaattatt gggacggacg aaagcatccc atgcaaattc
      481 tcggagactc cggtatttgg gaactcttta ttcctgaggt agctgcagga atgctctaca
      541 agtatgaaat taaaaccgga caaaacgaaa ttttattaaa gagtgatccc gttgctttta
      601 ctgcagaaca gcgtccttca acggcttcag tagtaacaga attgaacggt tttcggtggc
      661 aagaccaaaa atggctggaa gaacgagaaa atcgagatcc tctccggcag ccgatcaatg
      721 tttacgaagt gcatttaggt tcttggcaga gggttcctga ggaggacaac cgttcgttga
      781 cctatccgga actggctgac cgcttgatac catacgcggt tgaaatgggc tacacgcacc
      841 ttgaattgat gcctgtagca gagcatcctt tagacggttc atggggttat caggttaccg
      901 gttattttgc gccgacttct cgttttggta caccacatga gttcatggaa tttgtggacc
      961 gctgccatac atccggatta ggcgtgattc tggattgggt tccagggcac tttcctaaag
     1021 actctcatgg acttgcaggt tttgacggca ctgcacttta tgaacatgaa gatccacttc
     1081 agggtgagca caaagaatgg ggcacgctaa tttttaatta tgagcgtaac gaagttcgta
     1141 actttctgat tagtaatgct ttattttggt ttgacgaata tcatattgac ggcataagag
     1201 tagacgcagt cgccgcaatg ctgtatttgg attacaatag aaaggaaggg caatgggtta
     1261 aaaatagaca tggcggtcgt gaaaatattg gtgcaataga atttttgaag cagcttcatg
     1321 aaacgctgtt tcattaccat cctggaattc tctcaattgc agaagaatct accgcctggg
     1381 gcggtgtgag tcatcccaca tatgccggag gtttagggtt caatctaaag tggaacatgg
     1441 gctggatgaa tgacactctg cgatacattg aaaaagatcc ggtgcaccgc aaatacgatc
     1501 atcatttgat cacattttca ttgatgtatg cctttaccga aaatttcgta cttgcgcttt
     1561 ctcacgatga agtggttcac ggaaagcgtt caatgcttgg taaaatgcca ggtgacgact
     1621 ggcaaaattt tgctggactg cgaatgtatc tcgcttttca aatggcacat ccgggaaagc
     1681 agctgacatt catgggaaca gagtttggac aaagggacga gtggagtgaa aaccggagct
     1741 tagattggca tttaacgcaa tttccggatc atcaaaaatt acaggaatat gtccggaccc
     1801 ttaatcattt ctatctcagt aaacctacat tgttcaccaa cgattttgac tggacaggct
     1861 ttcaatggat tgatcatcat gatcaggaca acagcgtttt atcgttcatc cgccgaagtg
     1921 cagatgaagt atccgatcca gatttagttt ttattttcaa tttcacacca gtaccccgtg
     1981 atgattattt gactggattt ccggaatctg gtcattacag taaattattg gacacagatg
     2041 atccgaaatt tggcggatcc ggatacaacc aacggcagca aatcatcact agtccccaga
     2101 tgtggcaagg tcaaccgcac cgcgccacag ttaatttacc tccgcttgga tgtttggttt
     2161 ttgagaaaat ggcaggcatg taatcccggc ctgccagaaa accataacta accctcaaaa
     2221 tatataaaac cgaaaagaat gccgcagaca cgaaaagaaa tggagaatat ttgggaattg
     2281 gttgccacgt accgttcaat ggaccgggat gggttgatcg aatccattgc aaatcatctt
     2341 gaattctcgc aatgtaaaaa tcggtttact gcagaagatt ttgatgtcta ccggagtttc
     2401 gccctgtcag tccgggatcg gctggttgag ttctggaatg atacacagca aacctacctc
     2461 aaaaagaaat gtaaacaagt caattatttt tcactggaat atctgatcgg cagaagttta
     2521 aataacaatt tactcaacct gggcatcagc caggctggtg aagatgcaat ccggaaaatc
     2581 ggttatgatc ttgaagaact gcaggagctt gaacatgatg caggtttagg caacggtggt
     2641 cttgggcggc tcgctgcctg ttttatggaa tcaatggcga ctttgcaact gcctgcacat
     2701 ggatatggaa tccgctatga attcggaata ttcaaacagc aatttgaaaa tggggcacag
     2761 gtggaggctc cggacaactg gttggaagag ggatacccct gggaaattcc tcgatgggaa
     2821 gtggtttatc ctgtccattt ttttggtcgt gtaaaaaaat acacgaatga gaaaggtaaa
     2881 ttgtcccgga aatgggtaga aacagaagaa gtgttggcaa tggcttacga tgttcccatt
     2941 gccggatttg gaaatcatac agttaataat ttacgtcttt ggtcagccaa accttcaaaa
     3001 agttttgatt ttcaattatt taacagcggc gactatattc aggcggttga ggaaacacag
     3061 cgcagcggta ccatttcaaa agtattgtat ccgaacgatc aaggattttc cggaaaagaa
     3121 ttgcggctta agcagcagta tttttttgta gcggcttcac tgcaggacat cattctacgt
     3181 ttcaaggtgc attcggagac ttttgataaa tttccggaac atgtttcaat tcagctcaat
     3241 gacactcatc cctcaattgc aattccggaa ttgatgcgga tttttgtaga tgaggaaggt
     3301 ttggaatgga atgaggcctg ggctataacc acacgtgttt tcgcctatac caaccacacc
     3361 gtccttccgg aagcattgga gcgctggtcc gttgatttga tgggacgttt gctgccgcgt
     3421 caccttgaaa ttatttatga aataaatgat ttatttttgg aaagtgtgaa agaaaaatat
     3481 ccggatgacg cagatttatt acaaagaatt tcctttattg aggaaaatga tcacaaacaa
     3541 atccgtatgc cttatctttc aatcgttgga agtcacacga ttaacggtgt agcggagctt
     3601 catacagaat tgctgaaaac tacagttttt aaagattttt acaaactgtt tcctgaacgt
     3661 ttccaaaaca aaaccaacgg tattacaccc agactttggc tgcgaaacac aaatccggaa
     3721 ttatctgaat taatctcaga aaaaattggc gatggctgga ttacggattt acagaaatta
     3781 aggaaactgg aaccatttgc cgatgatcct gaattccagg aagcatggcg ttcagtcaag
     3841 cgcataaaaa aggagcaact ggcaaattgg cttaagcaga ccagtggagt aataatcgat
     3901 cctgaatcac tatttgatgt ccagataaag aggatccatg aatacaagcg gcaattactg
     3961 aatattttgc atgtgactta tctttacaac aaaatcctgg agcatccgga acttcctttc
     4021 gttccacgca caattttgtt agccggtaaa gcagcaccag gttattacat ggcaaaactc
     4081 atcataaact tagccaacga tgtggcgcgt gttatcaata gtgatcctgc agtgcagggg
     4141 cgtttaaatc ttgtttttgt accaaattat aatgtctcag ttgcagaaaa aataattcca
     4201 gcaacagata tttctcagca catttcaact gccggtactg aagcctcagg taccggaaac
     4261 atgaaattta ttttaaatgg agccctaata caggggacca tggatggtgc aaatatcgag
     4321 attgcagaag aagtcggcag ggaaaatatt ttcactttcg gtttaagttc ggatgaagtt
     4381 agtattttgg cagaatccgg ctacaatccc agagtttcct accaaaataa tccaggattg
     4441 atagaagcgc tttcgatgat caacacagga tattttaacc gtgacaagcc acacctttac
     4501 aatgatctct acaactcact ggtttttgag gataaataca tgctgctcga agattttgcg
     4561 agctatgatg aatgccggca gaaggtgatg aatacctgga aagatcagaa tacctggaca
     4621 agaatgtcaa ttttgaacac agctggatca ggtaagtttt ccagtgatcg tactattgct
     4681 gaatacgcaa aagatatttg gggacttgag gccgttactg ttgaattaca aggcaaaaaa
     4741 gttcgctgac agtataactc gtgaacaaaa gatcttccct gtagtggagt atgattacca
     4801 ggcattatgg acggcagtta actgaccggc tcactacttt tcaagcctac tgtgattttt
     4861 gcagaatccc tgaattgcgt tagtaatttc ttttcggtag actttgcggt ttcaaaatgt
     4921 cgctgtttca ccatatcgta accacgaatc tgttccggta tttttgctat ttccagcgct
     4981 atattgtggt ttttctttgt cagtccacgc agaagttcct taattgtttg ttcatattcc
     5041 tgaattaact gccgctccat tcggcgttcc tttgtttttc caaatagatc aaaagcagtt
     5101 ccgcgcagga acttaaaccg tgacagcatt ttcaacacag gaaaaatcca tgaaccaaaa
     5161 gcagatttga tcggctcccc agtatgggaa tcacgatgag aaaacaaagg tggtgctaaa
     5221 tgcagcttca accgaaagtc tccctcaaaa cggctccgta tttttttcaa aaaatctccg
     5281 ttagtataga gacgagctac ttcatattca tctttgtaag caagtaattt aaaataatat
     5341 cgtgctaccg cctcagtcaa ggataaacta cctggaagac ggtcggtttc aatggtgcga
     5401 acacgctcca ccagttgtaa atatcgcttg gcataagctt tattctggta tgcagtcaag
     5461 acgtccgcac gatgctgtat gagttcatcc aatccttcag taggatcctc aagaagaaaa
     5521 ccttctacga tcgaggccgt caactcccgc acgcgttttc catcatgtgc tgtcctcctg
     5581 ccccataaaa aagactgttt gttactctct actgacatgc cgttgatttc gatagcctgt
     5641 tcaatagaac tacgttccaa aggaattaaa cctcgttgaa aagcaaagcc gagcataaac
     5701 atgttggtgg caatagtatc accaatcaag gcagttgcca ggcgtgatgc atccaggaat
     5761 tcggtattgt ctgttccagc tgtttgctga attgcctgtt tgatttccag gcttggaaaa
     5821 agcatatccg catttttggt aaaatcaccg gtaatcattt cataactgtt tatcacaagt
     5881 tttgtatgtc cggattttat gactgaaaga gttttatgtc cacctgtcac caccaaatct
     5941 cctcctatca ccaaatcggc accttgagaa gcaatccttg gtgaatgaat atcttccgga
     6001 ctttttccta tgcgcaaatg tgataaaact gcaccgcctt tttgtgccaa tccaatcatg
     6061 tcaagaatac caattccctt tttttcaagg tgtgcagcca ttcccagtaa agcaccaatt
     6121 gtcacaattc cagtacctcc aactccggtc aaaacaatgt tatacgtgcc tttaattaca
     6181 ggtagctttg gttcaggcaa agaggtccat tcaacatcca tattggcaga aggatcattt
     6241 ttcctcattt ttccgccaat gacactcaca aaactgggac acaaaccatt gacacagctg
     6301 taatccttgt tacaggcaga ctgatcaatc acccgttttc tgccaaattg agtctcaaga
     6361 ggtaaaactg caacacaatt tgatttaaga ccacaatctc cgcagccttc acaaacatca
     6421 tcattgatat aaatcctgcg aggtggatcc ggaattaatc cacgtttccg tcgccgacgt
     6481 ttttctgcag cacaaacctg ttcgtaaatt agtaccgtca cgccctcaat ttcacgcaat
     6541 tctcgctgca gaatatccaa atccttgcgg tcgtaaacct ttacgtttgc cgggatgccg
     6601 gagctttgag taaatttttc cggttcatca ctgactacat ccaccctttt tgcaccttct
     6661 gcatacatct gctgaataat gctctgaaca gtcatcggtc catcaaaacc ttgtccgccg
     6721 gtcatggcga ctgcgtcatt gaacaaaatt ttgtaggtaa tgtttattcc ggaagatact
     6781 gctgcacgga tggccatggt tccggaatgg aaataagtac cgtctccaat attttggaaa
     6841 atatgtttac gtgtactgaa tgtagcctgt ccaacccagt ttgccccctc agcacccatg
     6901 tgagtgaatc caattgtatc acggtccatc cactgcacca tgtaatgaca accgattccc
     6961 gccattgcaa tgctgccttc cggaactacc gtggacgtat tgtgaggaca accggaacaa
     7021 aagtacggca acctcaccat tgattcctga ggtaacagtg aagagtccat gcggatttcc
     7081 atttcctgca aacgtttttt tagcttttca tctgtccgaa aagtcaaaat tcgtcgagca
     7141 atagttgcgg caatgtggtt aggatcaagc gctccagcag ttcgaaacaa tggttctcca
     7201 gtttcatctg tttttcctat cacttttgga cggttggaaa gatcgtaaag gaattctttt
     7261 atttgtggtt caattagagc ccgcttttcc tcaactacaa taatcaaatc cagccctttg
     7321 gcaaattcaa gtataccttg cggttccaac ggccaaggca tggctacttt gtagagtcgt
     7381 acaccaaact tttctgctcc agtttcgtca attcccagtt catcaaaagc tagtctagta
     7441 tcaaggtatg attttccaac agtaacaata ccaatttttg cgtttcctcc aggccatatt
     7501 gttcggtcca gtttgtttgc ccgacaaaat gagcgtactg catcaacctt aaattcgtgc
     7561 attctccgtt cctgcgcctg cggcgtgtcc ggaaagcgaa tatttaaacc atctacagga
     7621 aaattaaaat cattgggtaa ttccaatttg agtcttgcaa catcaacatt gattgtagaa
     7681 gttgcttcaa cggtatcatg tacacacttt aaaccaaccc agcatccgct gtaccgagaa
     7741 agcatccaac ctataattcc aaaatcaatt atttcctgaa cactggaagg attaagaacc
     7801 ggaatcatgg cgtctaacat ggcgaattca ctctgattac aagtagttga ggactctgcc
     7861 gtatggtcat cccccattaa tgccagcacg cctccaaact ttgatgtacc tgcataatta
     7921 gcatgtcgca atacatcacc ggacctatca acccctggcc ccttaccgta ccaaattcca
     7981 aaaaccccat catattttcc ttcaccactc aactccgcct gctgtgttcc ccaaagcgct
     8041 gttgctgcta aatcctcatt cactccgggc tggaatttaa cgtaatgttc atcaagaagt
     8101 tttttattac gtgccagttg ctgatcataa ccacctaaag gcgagcctcg ataaccactg
     8161 atgtaacctg ctgtattgtg tccagccttc agatccagtt cacgctgcac taagggcaaa
     8221 cgcaccagag cctggattcc agtcatgaaa gcataacctt ctctcaaagt gtacttatct
     8281 tccagtaaaa cagatcgttt ttc
//
LOCUS       DTVD01000134            2053 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_24118_length_2053_cov_353.000000, whole genome
            shotgun sequence.
ACCESSION   DTVD01000134 DTVD01000000
VERSION     DTVD01000134.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24118_length_2053_cov_353.000000"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..731)
                     /locus_tag="EYO68_04705"
                     /pseudo
     CDS             complement(1..731)
                     /locus_tag="EYO68_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510772.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(758..1759)
                     /locus_tag="EYO68_04710"
     CDS             complement(758..1759)
                     /locus_tag="EYO68_04710"
                     /inference="COORDINATES: protein motif:HMM:PF00710.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIC07141.1"
                     /translation="MKPKIHVIGTGGTISGTGSTATDAAYESGRVDASDLVSAIEGLE
                     RLADVHSETMFATGSENLGPHQWRILARRIEELTADSEVDGVVVTHGTDTLEEAAFFL
                     ELVCKSGKPVVLTAAMRPSTALSADGPANLFQAVLAATSPQLQSHGLLVVMNGLVIPG
                     WQIIKTDSVALDSFHAYPGGPIGKIVGESLLVFGSPNLSPLVGRFHDHLRNDDELPFV
                     DIVLLRAGCGDQPLADFGGNACKGLVVAGFGGGTMPDNLNGLVAETANSGCTVVVSSR
                     VSQVTVLAETMTSVKSKNVFPSGLLNPQKSALLLSLALDANLDALELTELFNVFVIQ"
     gene            complement(1793..>2053)
                     /locus_tag="EYO68_04715"
     CDS             complement(1793..>2053)
                     /locus_tag="EYO68_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIC07142.1"
                     /translation="PGCSVKKGDPGRDDFLKKMAEFYFTLEGLQKIKQKSGKTVGLMH
                     SLPRNEGEFDFAIDASEHELYFKQIGFSVPLRMSLLANICGV"
BASE COUNT          559 a          528 c          430 g          536 t
ORIGIN      
        1 tgagaaacag cggtcaggat catgatatcc ttgaatccaa gcacatttgc catgtaggct
       61 gttgtcaggt ctttgtcgat aactgcttca actccacttc gcaaaccttg actgttgcgg
      121 acaaccggaa caccgccacc tccgcctgca ataactatag taccattact tgataaggat
      181 tcaacaagag aaatatcgca aatgtgtttg ggtttcggag aggcaactac catgcgccaa
      241 ccacgtcagg aattttcttt catagtctat cccatttttt ctgtaagatc tgcggcttct
      301 ttctcgttgt aaaaataacc cacgggcttg gttggattct taaaaccagg atcatttgcg
      361 tcaacttcaa cctgtgtcac cagaccaacg acatggcgct ggtttccggc tttcctcaag
      421 gcattttcga aagcctgcac cagaagataa gccgtcacac cctgcgtgtt ggctacgcaa
      481 atgtccaaag acattggtgc aacctgttta tgggccaggg cttgacgcat cagaaccttg
      541 ccaaccccgg ggccgttacc atgggtaatt atcaactcgt tttcaagttc taacaacggc
      601 aacaaaacat ctgccgtttc ttcagcaatg gcaacctgtt cttcagatgt accttttatt
      661 ccggccgggt gaatcgcatt gccgccaaga gcaacaataa ttcgtttggg aaattctttg
      721 tttgaattca tgtaataacg cctaaatggt attgatttta ttgaataaca aaaacattga
      781 acaattcggt taattccagt gcatcaaggt ttgcatccag agccagagac aaaagcaatg
      841 ctgatttttg cggattaagc aaacctgacg gaaaaacatt tttactttta accgaagtca
      901 tggtttccgc tagaaccgta acctgtgaca cccgagagga aacgacgaca gtgcatccgg
      961 aatttgccgt ttcagcgact agtccattca ggttatcagg cattgtaccg cctccaaacc
     1021 ctgccaccac caatccttta caagcatttc ccccaaaatc ggcaagtggc tgatcaccgc
     1081 aaccagcacg aagtaaaaca atatctacaa acggcaactc gtcatcattc cgcaaatggt
     1141 cgtggaatcg tcccactagc ggagaaagat ttggtgatcc gaaaaccaaa aggctttcac
     1201 cgacaatctt tcctatcggc ccaccgggat aagcatgaaa agaatccagt gccacagaat
     1261 cagttttgat gatttgccat ccgggaatca ccaaaccgtt cattactaca agcaatccat
     1321 gactttgaag ttgcggagag gtggctgcaa gaaccgcttg aaatagattc gcagggccgt
     1381 ctgcactaag tgcggtgctt ggacgcatag ccgcggtcaa aactaccggt ttcccggatt
     1441 tgcacaccaa ttcaagaaaa aaagccgctt cctcaagtgt gtcggttccg tgggtaacaa
     1501 caacgccgtc aacttctgag tctgctgtta actcctcaat tctacgtgcc agtattcgcc
     1561 attggtgcgg tccaagattt tcagaacccg tggcaaacat ggtttctgaa tgaacatcag
     1621 ccagtctttc caaaccctca attgcactga cgaggtcgga agcatctacg cgcccggact
     1681 catacgccgc atctgttgcc gtagaaccag ttccggaaat cgttccgccg gtgccgataa
     1741 catgaatttt cggtttgata gtgacctctt ttcaactgtc gtttaccgtg cttcaaacac
     1801 cgcagatatt tgccaataag gacatccgta gcggcacaga aaatcctatc tgcttgaaat
     1861 aaagttcgtg ctcgctggca tcaatcgcaa aatcaaactc accttcgttc cgtggaaggg
     1921 agtgcatcag gccaaccgtc ttgccgcttt tttgttttat tttctgaagt ccttcaaggg
     1981 tgaagtagaa ttcagccatt tttttcaaga aatcatctcg tccgggatct ccctttttca
     2041 cagaacaacc ggg
//
LOCUS       DTVD01000135            2046 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_24260_length_2046_cov_331.791562, whole genome
            shotgun sequence.
ACCESSION   DTVD01000135 DTVD01000000
VERSION     DTVD01000135.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24260_length_2046_cov_331.791562"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            453..1055
                     /locus_tag="EYO68_04720"
     CDS             453..1055
                     /locus_tag="EYO68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07143.1"
                     /translation="MYCSFFDVDGVLLDFEGGFMKAIKDYFQLDIPDDFSSKSFWFSD
                     LLTKEQVTEGWDYFVHSPEFEQLQPMVDPEHFNAKFGAYPVHFITNIPPDCLERRKTN
                     LRNAGFKFDSAHCAGFINYDGHPVQTKADVILKLQQEEEKILFVDDHPDNCLDVRESF
                     PDAEIWLMSRPFNDDFEHPEIRRAKDWSDVLENSNIIPAR"
     gene            1139..>2046
                     /locus_tag="EYO68_04725"
     CDS             1139..>2046
                     /locus_tag="EYO68_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07144.1"
                     /translation="MLFQKILRKQFVPAVIILLLLVPGTYAQEEAAVQTGGSNAAWRI
                     GFWEILHQTFSSDDLHPFYQMTGTNRFKGGKGFFGLTTLDPEKNNLIPNIYNKNDDEH
                     GSKDGFLTKLFSNIPLLKGLPSFSLEYILPTDFDVGIGLAFAYTNIWLDDTKVRAATS
                     GADPAYATPLLRMASHFYMFSASLHPFGVPRPDDMDIFLGFGLSRVESTLRYGIRDNP
                     TIEEYASVTKTELSGSSGTMPFRRMGIASGGDSFGFMLEFLFPGKNEIIDNPFATNTI
                     IDSTVYDSTYNDRGGALPSKVGMPGG"
BASE COUNT          610 a          387 c          411 g          638 t
ORIGIN      
        1 taaccactca tttccaggtg atttacattt tactgtcgat gagtagttca atttcataca
       61 ataatgcttt acttttgtta aaaaaatctt taaaataaat ttaccttttt aagttcgcca
      121 aatattatgt agtctaacaa aacagactgg cgaaccataa aacacccccg caaaagcctt
      181 gtggtgtatc agggaaatct tggttttcag ctcctcccta caaaatgttg aaagatttca
      241 tgtaccagga aaatcgatgc agactaaggt tggaagatgt ctggtcacta ttatagctaa
      301 cgggggtgaa acaagcttaa atcaagataa cctccggtta atcccttctt ttttaatttt
      361 aatatttttt gtacaaattt agcattgcag actttgtgtt tatgcaactt gttctcagcc
      421 ctttaattta gtattcccat agaagaaaaa aaatgtattg tagttttttt gatgtagatg
      481 gtgttttgtt ggactttgag ggtggtttca tgaaggcaat caaagattat tttcaattag
      541 atattcctga tgatttttca tccaaaagtt tttggttttc tgatttactg acaaaggagc
      601 aagttacgga aggatgggat tattttgtcc atagtccaga atttgaacaa ctccagccaa
      661 tggttgatcc ggaacatttc aatgctaaat ttggggcata tccagtacat tttatcacaa
      721 atataccacc ggattgtctg gagcgtagaa aaacgaattt aagaaatgca ggattcaaat
      781 ttgattctgc acactgtgcc ggatttataa attatgacgg ccatccagta cagacaaaag
      841 cggatgttat cctgaaatta cagcaagaag aagaaaaaat tctatttgtg gatgatcatc
      901 cagataattg tctcgatgtc cgtgaatcat tcccagatgc agaaatttgg ttgatgtccc
      961 ggccttttaa tgatgatttc gagcacccgg aaattcgcag ggccaaagat tggagtgatg
     1021 tacttgaaaa ctccaatatt attcccgctc gctaaccaaa cctacctgga ctatctggat
     1081 gtttccgaat tgatgatgta ctactcggtt tttgattatt gatgattctg atgtgttcat
     1141 gttgttccaa aaaatcttac gaaagcaatt tgtacctgca gtaattatac tgctattgct
     1201 tgtgcctggt acttatgctc aggaagaagc tgccgtgcaa accggaggca gtaacgctgc
     1261 atggcgtatt ggtttttggg agatccttca tcagacattc agcagcgatg atttacatcc
     1321 attttatcaa atgactggta caaaccggtt caaaggtgga aaaggttttt ttggcctgac
     1381 aactcttgat cctgaaaaaa acaatttaat tcccaatatc tacaataaaa atgacgatga
     1441 gcacggcagt aaagacggtt ttttaaccaa attattcagc aatatcccat tgctaaaggg
     1501 actgccttct ttttccctgg aatacatact tcctacagat tttgatgttg gcatcggctt
     1561 ggcctttgcc tacacaaata tttggctgga tgatacaaaa gtcagggcag ccacctcagg
     1621 tgccgatcca gcatatgcaa cacctctgtt gcgtatggct tctcatttct atatgttttc
     1681 tgcctcactt catccatttg gagttccaag accagacgac atggatattt ttttgggttt
     1741 cggactgtca agagttgaga gtactttgag atatggaata cgcgacaatc ccacaattga
     1801 agaatatgct tcagtcacaa agactgaact cagcggttca agcggaacga tgccgtttag
     1861 gcgaatggga attgcgtccg gaggagacag ttttggtttt atgctggaat ttttattccc
     1921 tggaaaaaat gaaatcatag acaacccatt tgcgacaaac accatcatcg attcgactgt
     1981 ctacgactca acttataatg accgcggagg agcattaccg tcaaaggtag gaatgccggg
     2041 aggaat
//
LOCUS       DTVD01000136           26660 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_243_length_26660_cov_352.078632, whole genome
            shotgun sequence.
ACCESSION   DTVD01000136 DTVD01000000
VERSION     DTVD01000136.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 26660)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26660)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26660
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_26660_cov_352.078632"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="EYO68_04730"
     CDS             <1..450
                     /locus_tag="EYO68_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC07145.1"
                     /translation="GSAAGLDGSMAKAVERGENWFGYYWSPTSMIGKYNMFKVPFGVP
                     FAGSKNWDGCIVKPEQECANPKPSSWTKSVVNTIVTDRFKKAGGPAADYFTKRVYPGP
                     VMNGMLVYMADNQAGGADAAVEFLQKHEDVWTKWVPASVASKVKSSL"
     gene            523..1413
                     /locus_tag="EYO68_04735"
     CDS             523..1413
                     /locus_tag="EYO68_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HIC07146.1"
                     /translation="MSWFEKIPTMDRQELRDLKKYIDISFKDFSRTYGDGIETFFDPL
                     LYFLVWFEKLLINAPWPLIILVIAIMAWIGSRSWFVIAGTVCSFLLIGYFGMWEDTMS
                     TLAIISICTLLCISVGIPIGIFMSRSDRLQSMVNPVLDVMQTIPSFVYLIPVVMLLGI
                     GKVPGLIAVCIYALPPIVRLTNLGIRLVDKEVLEASESFGASYSQKLFGVQIPLALPN
                     IFAGVNQTIMMALAMVVIASMIGVKGLGVPVLRAVSNQYLALGMLNGLAIVALAIIFD
                     RISQSFGKRMQKHLESAHGV"
     gene            1403..2452
                     /locus_tag="EYO68_04740"
     CDS             1403..2452
                     /locus_tag="EYO68_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC07147.1"
                     /translation="MVSETKVAIKNLYKIFGKNPTGMINHVQNGISKQELLEKYGSVL
                     ALNDVNIDIPARGVQVIMGLSGSGKSTLIRHINRLIEPTSGEIIVDGQNVLDMSKTEL
                     REFRRHTASMVFQRFALLPHHTIMENVSYGLTIQNVSKQDASERSQQWIDRVGLAGYE
                     NYYPNQLSGGMQQRVGLARALATDAEILLMDEAFSALDPLIRTDMQDVLLGLQEELHK
                     TILFITHDLDEALRIGENIAILRDGLVVQKGTPQEIILNPVDKYVTDFIKDINRGRVL
                     RVSSIMESKKLKNGPEIEKNMIIEDALQIISNAEVSEAIVTENGKTIGSVKLGRMITA
                     IARPSEKTGQITNYR"
     gene            complement(2662..4194)
                     /locus_tag="EYO68_04745"
     CDS             complement(2662..4194)
                     /locus_tag="EYO68_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIC07148.1"
                     /translation="MSRERKRKGRAQSRTNNGIQQQPWKQPRNPYSPFEIASADQIEE
                     IHQASLRILRELGIAFLDDEALDILKEHGAEVDRSSQMVKFSPELIEEYIAKAPSQFT
                     LHARNPAHNLEVGKNWTLFSMVGSTPNCSDTDNGRRPGNFKDYQNLIRLAQYFNIIHM
                     TGGYPVEPIDLPANTRHLDCALTHLTLTDKVFHAYSLGKQRISDTIDMLCIGLGTTRE
                     ELKTRPSLISIINTSSPLRLDGVMIQGMLEMIRNGQSVCVTPFTLSGAMAPITLAGAL
                     SLQNAEALASLAFTQMEAPGSPVIYGGFTSNVDMKTGAPAFGTPEMAKTTLIGGQLAR
                     RYGLPYRASNVNACNTVDTQAGYESMMALWPTIMSHCNFVKHAAGWLEGGLCASFEKV
                     IVDVELLQMMSAFLDELSFSEDEMAFEAIADVGPGGHFFGTQHTLDRYETAFYPPILS
                     DWNNFGQWTEDGGLDTTQRANHIWKRALEDYQQPPMEESRVEELEAFVARRKEKPGIS
                     MH"
     gene            complement(4313..5560)
                     /locus_tag="EYO68_04750"
     CDS             complement(4313..5560)
                     /locus_tag="EYO68_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC07149.1"
                     /translation="MPLASVQEIKAGRAIQHGAMEFSTAPVDLDTVRRYRLQRLRTKM
                     GELDVSGLLLFNQINTRYAVDATNMQIWCSHYETRCVFVALDGPVVLFDYANHPYLSE
                     GIPTIDEYRVLPAFSFFGAGERCHDFVKEFAAQIEGLMKNHGGGNRRLAIDILSHTAC
                     DALRETGLELVEGEQIAETARAIKSKEELVLMQASMDVCEAGVLSMHEALEPGITENG
                     LWAKLHETNIRLGGEWIETRLLSSGPRTNPWFRECSMRQIEKGDMVSFDTDLIGPYGY
                     CSDMSRAWVCGEKPNNEQRRLYTKAFEQIEHNISVLKAGMTFREVSEKCWPIPDEFLA
                     NRYTTLIHGVGLADEYPNIIHCEDFDDKGYDGLIEVGMTLCVESVIGSEGGRECVKLE
                     EQIIMTETGVERQSSYPFAMEML"
     gene            complement(5705..6955)
                     /locus_tag="EYO68_04755"
     CDS             complement(5705..6955)
                     /locus_tag="EYO68_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HIC07150.1"
                     /translation="MQYYSQQRLPTHVYTQPETYVLSRLPVDQASTLIPDAYTAADFF
                     ALEQEKIFATAWVAVGCLQQVQNPGDILVTEVAGRSILVMRDKTGKLGAFYNVCRHRG
                     SKLLEKSCNIKSLHCPYHAWVYGLDGQCLGTSMFRNEKIVDTVPSNGGETDLSDTALF
                     DKKNYGLLPLSVQTWGFLVFVNLSAQTTPLEEWLGDIPQKFQSHVLENWEIFGEKNYD
                     VASNYKLIEENFMEYYHLPFVHPELTNVSRMEDHHRSQGAGMYTGMLTTPVSRDVDSV
                     WLNLPAVQGLNKEHLEAAYHICIFPNVTITILPNHAFCMITDPIRANQTRERTFLLTP
                     PGTLDNESHTSMFLELVKFWDMVNTQDLGIVERVQLGLSNTAFTGGRMCPKFEEPLHR
                     YQNWVADRMCGIHRVPSGDNDARV"
     gene            complement(6973..7878)
                     /locus_tag="EYO68_04760"
     CDS             complement(6973..7878)
                     /locus_tag="EYO68_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIC07151.1"
                     /translation="MSANILDGKAIAKDILDEVSSKVSELKTSGWDPKLISIKVGENP
                     AVDLYIRNQRLKAEKIGIIFEEKEYPNDISANELQAAIANFNVDPKVTGIFLQRPVPK
                     SISIKTLQKAIHPLKDVEGMHPASIGNIVYNEMELGPCTAMASVELLRRTGQDLKGLE
                     VVVIGHSEIVGKPIAFLLMAEGATVTVCHHMTKDVADHSRRADAVFVAVGKPNLITGD
                     MLKSGSTLIDIGINRVTDDKGKSRIVGDSDFDSCSKKAGWITPVPGGVGPVTVSCLMR
                     NTVSAAQKLKSYYESQFQNSIDAPF"
     gene            complement(7875..8402)
                     /locus_tag="EYO68_04765"
     CDS             complement(7875..8402)
                     /locus_tag="EYO68_04765"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC07152.1"
                     /translation="MINYEKEYQNSRNVCGEPFPEIVEFFENYDDECATVLDSGCGQG
                     RDALFIARKGHSVLGVDTAQTGIEQMLEEAESENLAVDGVIADITNYEAPDLYNIVVI
                     DSVLHMLQNDEIRNAVLEKSSNAVSKAGFILIADTPKHKSLICDYFKSVPKVWKIIKN
                     EKNFFFAHKIALVKS"
     gene            complement(8406..10868)
                     /locus_tag="EYO68_04770"
     CDS             complement(8406..10868)
                     /locus_tag="EYO68_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC07153.1"
                     /translation="MKSHTQVVVIGGGVVGCSVLYHLTKLDWKDAVLLERSELTSGST
                     WHAAGGMHTINGDPNVAKLQQYTIDLYQEIQEISGQDIGKHVTGGIMLAATKERFEWL
                     KSIHARGRYLGLETEIISPKEAQKIMPLINPEHFVGALHDSIEGHLDPYGTTWAYAKA
                     AKKQGGEIYQNVRVTDLQIHPQGGWRVILENGDSGEQEHPVRVEEIHAEHVVNAGGLW
                     AREVGRMVGLELPVLAMEHMYFLTEDMPEVMAFNQENELELPAVIDFDGEIYLRQEQQ
                     GMLMGTYEKACKPWSPKVTPWDFGQDLLPPDLERIAPSLEVGFEHFPVFENAGIKKVI
                     NGPFTFAPDGNPLIGPVRGLPGFWCACAVMAGFSQGGGVGLALANWMIEGDPGFDVWG
                     MDVSRFGDWATLSYTNTKVQENYSRRFSIRFPNEELPAGRPLRTTPVYDKMLTEGAQM
                     GSSFGLEVPLWFAPKGVTDKFSWRRSTDFEHVAEEVRAVREGVGVLEISGFAKYTVVG
                     SGASDWLDQILACRIPKPGRMTLAPMLNENGKLIGDFTLANLDDDEFFIAGSGIAEEY
                     HLRWFEQHLPESANLEVIPHRADILGLSIAGAKSRELLAKITDEDVSRDAFRFMDIRE
                     MNIGMVSCLVGRVSYTGDLGFELWMEADVQRYVLELLLREGEEFGIRLFGLRALNALR
                     LEKNYGSWATEYRPLYGPLEAGLSPFVALNKDADFIGKDAAIQEKSDGGILRLCTFVV
                     EAKDADVIGDEPIWFGNEVKGWVTSGGFAHNSGVSVAMGYVPKEIADEKQGWFVEILG
                     ERYPATLQTEPLFDPKGEIMRG"
     gene            complement(11156..11452)
                     /locus_tag="EYO68_04775"
     CDS             complement(11156..11452)
                     /locus_tag="EYO68_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07154.1"
                     /translation="MLRYYCLILIFALFIGGCQVQVRYPTEFPSKTTVTHEENYWFWG
                     LVGEKKYEVYDLCPQGRVYEILIYSTYMQSTYTVLSLGIYSPRTVLIVCSIRGE"
     gene            complement(11506..11985)
                     /locus_tag="EYO68_04780"
     CDS             complement(11506..11985)
                     /locus_tag="EYO68_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIC07155.1"
                     /translation="MKLGVLVYLENNDEHVLMIHREKEDEHKGLWLAPGGKVEPNEAP
                     FETAVREMLEETGLKIEDPELKAVLSFPDQGDSPFGDEWHVFVFYSNCYSGTLTEDCP
                     EGQLDWIPKDNLTELASWEGDRLFTPKILEPGCFSAKFLYSGKTLLDYTFSKSKLRL"
     gene            12005..13030
                     /gene="fliM"
                     /locus_tag="EYO68_04785"
     CDS             12005..13030
                     /gene="fliM"
                     /locus_tag="EYO68_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIC07156.1"
                     /translation="MPQSKKELNAILKRISVEQDKRLNPEEDEKTVVLPYELGFQAPI
                     IRGRMPGLEAVYDRFARIYAQTFSHHVRRPVTMTRRATELVSFRDYLAAISTPASLTV
                     FGMNPLHGHALFVFEHHLVYILIDMLFGGNGWFENPPPKRELTGIEIKMLSKVVHSAL
                     EDLGKAWKRLTPLEPYFDRMEVNSKFTAIVPPDEVVASTTFDVEINRVPYTMSLCLPY
                     SMLDPVRTQLEAGKTSYDEEVNAVNVSRLEENLLNSKVGVKVDLGEARMSLRSFLTLK
                     EGDRILLNQDQNKPLKVLVQDKLKYLATQGAYKGKNAVQITKLIEPPPRFSDLLDQPA
                     KDTAEDS"
     gene            complement(13044..13319)
                     /locus_tag="EYO68_04790"
     CDS             complement(13044..13319)
                     /locus_tag="EYO68_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIC07157.1"
                     /translation="MNKSELIEELAAKTGYNPKLTDKMVRIFFDRIKAALSAGDKVEI
                     RGFGSFTLKKYKGYIGRNPKTGETVEVKPKQLPVFRTGKDLRSRVNQ"
     gene            complement(13455..15221)
                     /locus_tag="EYO68_04795"
     CDS             complement(13455..15221)
                     /locus_tag="EYO68_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIC07158.1"
                     /translation="MNNFKKHWQRKLNKFLSRGLAGSDTDLLSRYQAVSAREAVVMMT
                     AALAIGLGCGVLAVGLNWSVHSLRLFISDLDLGWRAIFFPALGAGIAVFLVRSLMHDS
                     AGHGVSDVIKAIAIGSEGLRRRMIFSRFFGSFLTVGSGGSTGLEGPIVSIGAALGSTL
                     GRWLNMNERHKKLLVGYGAAGAVAGIFNAPLTGLIFSLEIIIGEWTYLTILPTIIAAV
                     SATELSRWITGNKIAFFQEIAGFSLTSLLACFVLGALTGVISILFARSLLLWEKFFTK
                     VSLHHWIRAAIGGLFVGTLGFFMPEVLSEGYSATQVFLTDSIRPELAFVLLFVLLKFL
                     ACGMTLGSGGIGGVFAPSLVIGSGVGLAFGMIMHSLPLDGVAEDAAFALAGMAGMVAG
                     VMHGPLTGIFLVMEVTRGYSMILPLMLTASSAMVVSSFFEIGSVYTQDLIKQGSLIKR
                     GTDRYLLHHLNIGDILDKDFITIREDLLLRNFIEEFKKARRNYFPVLNKENKCVGVVF
                     LDDIRPHLFDRSLYDLVTMGSVMRNLPLISLNEPISAALDKFETSKAWSLPVVEGDQF
                     LGMLSKSTLFDHYRRELQIQSI"
     gene            complement(15184..15864)
                     /locus_tag="EYO68_04800"
     CDS             complement(15184..15864)
                     /locus_tag="EYO68_04800"
                     /inference="COORDINATES: protein motif:HMM:PF00359.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIC07159.1"
                     /translation="MSKWLVVSDVVQLLHVPEATIYRWIRQGDIPCIIRRGKYYFNQS
                     TLISWADSKHIHLEEHKRRSRKSKEQVSQSRLIEALKAGNVFHAVPSSSIESLFQQVS
                     VQMDLPQPIGDSLAELLLQREHLSSTGIGNGIAIPHCKTPLGKQINKSMVGTFFLENS
                     LDFMAPDGLPVFVVFVLLSVDSFHHLQLISQLARILSQSQMIDFLNNSPSLDNLVEQF
                     QKTLAEKA"
     gene            16221..18263
                     /gene="dnaX"
                     /locus_tag="EYO68_04805"
     CDS             16221..18263
                     /gene="dnaX"
                     /locus_tag="EYO68_04805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:PRK05563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIC07160.1"
                     /translation="MCRIPQPLFVNIGIPLMSYVVLARKLRPSRFDDLIGQETAAQTL
                     RNAVLSDRVAHAFLFTGSRGVGKTSAARILTRAVNCLNPDNGDPCNRCENCTEINQNA
                     SADVIEIDAASNRGIENIRELRENVNYVPAKCRYKIYIIDEAHMLTLESFNALLKTLE
                     EPPPYVKFIFATTDPHKIPPTIISRCQRYDFLRISIKKMADFLETVVKNENLELSRKA
                     IEMICRNSVGGMRDALTAIDQILSFTGTSATDQKVAQILGILDSESRFAFIEALLEKK
                     DGEAISYFQKLQEHGHDVNDVLSDLLQTIKTIALVRTLGMNQALFQDIPADDLETFST
                     LSKIVSADELQQIFHILLELEEQMKRSAHTKICFEMAILQIASIEPLVGLPEIISEIQ
                     NIRKSKSAGNSNTENTDSVIEGPAELSGPEKDNSGANQQHSFLKMSGAHKPLTDATVI
                     KNILKSDKTESAADEPAKLNESSPDPKMTEKQHSVTNQDNYSHVDEEKPYVAETEVSQ
                     VKAEQTENADKEMSPEAQNNINQNQEIVKLAAKKNIEETSMEPPEEWCNFSEEVQKLS
                     SKLASFVRNAVPHNLTGSQLILSFKSGNYASMLTEDNRELLEKIASDWTGHSVHVSCE
                     ITPISASQLTIVEHEQILLERKNELKRKNARAFPQVKDILSVFTNSRVTNIELSDA"
     gene            18323..18658
                     /locus_tag="EYO68_04810"
     CDS             18323..18658
                     /locus_tag="EYO68_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIC07161.1"
                     /translation="MIDMNEMMKKAQELSETMKQQQEELAKQIISVSVGGEMVKMTFN
                     GKQEAISIEIDPEVVDADDIETLQDLILSAVNEGLRQSQQNMQNSLGQQIGKMTGGLD
                     LSSILNPKS"
     gene            18946..19704
                     /locus_tag="EYO68_04815"
     CDS             18946..19704
                     /locus_tag="EYO68_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07162.1"
                     /translation="MAADQVLLENYSQDANPVFRIYDWECTTLSLGRNEILDNLIDLD
                     GCGRLEIPIVRRSTGGKAVLHGFDLTYSIVGGVLDQHFSGGVLDNYRYLAKGFYAFFK
                     KLGLNPVLKAIDRQKKKSDSHICFADPAVYEILVEGRKIIGNAQRVRNFRSSDSSSCR
                     VFLQHGSIPLKDSIPQIIQIFPHAAEDDLRREMHSLETAGVYPGLAQKKLRQMFLDCL
                     QETFQLKWKRQFWSVEELKMISERENSFQNLNIN"
     gene            complement(19799..20050)
                     /locus_tag="EYO68_04820"
     CDS             complement(19799..20050)
                     /locus_tag="EYO68_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HIC07163.1"
                     /translation="MLKAISELVVKLPWSMIILFCLTLGLAPFFPIPHVWEKLKMFSS
                     GPLVRPIDWFDLIMHAAPWILLLIKIVLTMKENGVTDNS"
     gene            complement(20145..20627)
                     /locus_tag="EYO68_04825"
     CDS             complement(20145..20627)
                     /locus_tag="EYO68_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC07164.1"
                     /translation="MKIREAVKEDFEQIWIIFQHIVSAGETYAYPVETSKEEAFQIWM
                     EQPHKTFVCVEQQHIFGTYYLKPNNPGQGSHVCNCGYMVSPDARGKGLATLMCEHSQK
                     AAKELDYKAMQFNLVVVSNEQAIRLWTRLGFETVGRITKAFLHPKLGYVDALLMYKWL
                     "
     gene            complement(20642..21277)
                     /locus_tag="EYO68_04830"
     CDS             complement(20642..21277)
                     /locus_tag="EYO68_04830"
                     /inference="COORDINATES: protein motif:HMM:PF01810.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIC07165.1"
                     /translation="MDFTQWSQLALICVMGAMSPGPSLAVILRNTISGGRTQGLMSGV
                     GHGLGITFYSVVAITGLVALLKTVPNFFLIAQIIGSLFLILLGGKMIISGIGGEQDNG
                     ELKQSTITGYRGFSEGFLIAFLNPKIAVWLLALFSQFVQPDADFTEQLVMVSTVGGVD
                     TSWYCLVAFLASSGGLVKALQRNAKRIDLIMGVLLILLAAGMLWRAFTLLS"
     gene            complement(21426..23882)
                     /gene="priA"
                     /locus_tag="EYO68_04835"
     CDS             complement(21426..23882)
                     /gene="priA"
                     /locus_tag="EYO68_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIC07166.1"
                     /translation="MIVEVALNLPIRKSFDYHWPDKLTLVPEKGLQVLVPFGAQKKGG
                     VIVRVKKHSGITRLKNVETLVDEEPLFSEELLKLTKWTSEYYFCAWGETLNAAIPGGL
                     ALRLRTTYTPQTTSLPGLDTLSQKPQILIDTQSTWTQQEWLQCNPDERDHQQLRNWLS
                     KDHVQSTQVLLGQKTKPKMERWIRLLKPDNPKNSVSRRKTKRQQIFEILNENREICWS
                     DVQNRVNAPSQALKKLKEEGHIEFFEKRVYRRFMEGGLPEIEPFKELTPEQKSVFEKL
                     SDSLQNGTYRTYLLEGITGSGKTEVYLHAVREAQKLGKSCLILVPEISLTPQLVNRFR
                     SRFGDHVAILHSGMDDGERFDEWSRVRHGFASIVIGARSAVFSPMKNLGLIVIDEEHD
                     PSYKQGETPRYHGRDVAIFRGYEAGATVLLGSATPSLESSNNVSNGKYELLSLPSRIN
                     QALLPEVRLLDMKTVPGQKGSPYFSSELVEALRLRLLKKEQSIVFLNRRGFAPLVRCS
                     KCESTFTCPNCSLSLVYHQVANQVQCHQCDFVKPLVQRCPECGSDHAPIIIGTGTEQV
                     EENLKMFFPAARILRMDRDTLHGKHALSKMHDRIRRHEVDIVIGTQLVTKGHDFPEVT
                     LVGVILSDLSLNIPDFRASERTFQLLTQVAGRAGRGYKPGEVLIQTHNPRHHSLLCAK
                     EHDTRQFREMELERRQNLRMPPFHSLTLVVCSSPNEKRAENLIWEIAEKIQKFSSNKN
                     YSNTAQFTSEIKPIDSVQVIGPIEAPMKKLRNRFRWQLLLKADNVRPILRLLKQVLET
                     PPSTRRDELIQIDVDPHHLM"
     gene            complement(23886..25358)
                     /locus_tag="EYO68_04840"
     CDS             complement(23886..25358)
                     /locus_tag="EYO68_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07167.1"
                     /translation="MTSRDFYIKYLLSCLEISQQLESESGSSIWFAHLNLHDFTEVEI
                     PDGGKIADYLLSEMDLQVVQFFLLLIERKRLETWTENSEQVSFQELIEIKLQKSNHNN
                     KNATLKKQGSVWKNKLDPETLKGIIVQNPDAPLESVAKNRHAVIVNPEQSLRLEVLNI
                     PKPWGHEGWYTGVEKRGVVKVTDEYGKTELPYALNIFKKQVLADHPESLILLKTLNPV
                     SEDVIGDLYYEMHEKKWEVYVVTEIDQTAWPSGTGIIKAGLHPEKIKDYQEIHGSKWV
                     EILLKNFRETIGEYEKIRRQIDDSTEDIPNELHEQELKLRQKASNFVGDCQVKVGDII
                     SFPVFQMHSLRHGIKVIEFQTPHYERLILMFAQKVLTQNHWDTDDALNKMLPVVYEPP
                     ELECLHKSSGLLIERFVDFPQFTADRICLEPETIWEDQLDGKYHLLITISGQASIIPK
                     SGSPVKLNREEALFLPVGVGSYRLESTGEIPLICLKAIPK"
     gene            complement(25362..26282)
                     /locus_tag="EYO68_04845"
     CDS             complement(25362..26282)
                     /locus_tag="EYO68_04845"
                     /inference="COORDINATES: protein motif:HMM:PF01177.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07168.1"
                     /translation="MKLVITDSGLGGLSVCAKLLQLLSEPAGANHPNYPADDLQITYI
                     NAVPSNNRGYNSMSGRAEQLKTFEIILRNTEKIFAPHHIFVACGTLSVLLDDLEIPSE
                     KTVKIEGILQIGVKMLLSSLLNDAQSSAIIFGTPTTINTETFQNELFKKGVQEIRIIS
                     QGCPDLASQISNDPDSSFVEERIRHWVQKAMLKLPEKYIDTLLIFLACTHYGYRQDLF
                     QKAFNEEGFCNITLLNPNLAAAENLVKTVSNNLNPSSTESKAFSVEFVTPYAIPEQEI
                     ITLTQLLSPISPATADALNNARICPELLNP"
BASE COUNT         7846 a         5686 c         5370 g         7758 t
ORIGIN      
        1 ggttcagcgg caggtcttga tggatcgatg gctaaggctg ttgagagagg tgagaactgg
       61 tttggatatt actggtcacc aacctctatg attggaaagt ataacatgtt taaagttcct
      121 ttcggtgttc catttgcagg tagcaaaaac tgggatggct gtatcgttaa gccggaacag
      181 gagtgtgcaa acccaaagcc ttcttcttgg accaaatctg tagtaaatac tatagttacg
      241 gatcgtttca aaaaggctgg tggacccgca gctgactatt ttaccaagcg tgtatatcca
      301 ggtcctgtta tgaatggcat gctggtttat atggcagata atcaagctgg cggtgcagat
      361 gcagcagttg agtttctgca gaaacacgaa gatgtttgga ccaagtgggt tcctgctagt
      421 gtagcatcaa aagtaaaatc ctccctctaa taaaaaatta acggacctga cttttatatt
      481 tatcggcggg tccgttaatt cttttatcca aaaattactt caatgagctg gttcgaaaaa
      541 attccgacaa tggatcgtca ggaattgcgt gacttaaaaa agtacattga tataagtttt
      601 aaggattttt caagaacata tggtgatggt atcgagacct tctttgatcc attactttat
      661 ttccttgttt ggttcgaaaa attattaatc aatgccccct ggccgctgat tatacttgtg
      721 attgctataa tggcctggat cggctccaga agctggttcg taatagcagg cacagtttgt
      781 tcttttcttt taatcgggta tttcggcatg tgggaagata ccatgtcaac actggctatt
      841 atttcaattt gcactttgct ttgtatctca gtagggatac cgattggaat atttatgtca
      901 cgctctgatc gtttgcagtc catggtcaat cctgtattgg atgtaatgca gactattcca
      961 agttttgtct atttgattcc tgtcgtgatg ttgttaggga ttggaaaggt gccaggactt
     1021 atagcagtct gtatctatgc cctacctcct atagtaaggc tcacgaatct cggaatacgg
     1081 ctcgtagata aagaggttct agaggcctct gaatcttttg gagcgagcta cagtcagaaa
     1141 ctatttggtg ttcagattcc tctagctttg cctaatatat ttgcaggtgt taatcaaacc
     1201 attatgatgg ccttggcaat ggtggtaata gcttcgatga taggcgtgaa aggcttaggg
     1261 gtaccagttc tacgtgctgt ttccaaccag tatctggctc ttggaatgtt gaatggtttg
     1321 gctatagttg ctctggctat tatatttgac aggatctcac aatcatttgg caaacggatg
     1381 cagaagcacc tggaaagtgc ccatggtgtc tgaaacaaaa gtagccatta agaatcttta
     1441 taagatcttc gggaagaatc ctactggtat gatcaaccat gtccagaatg gtatttccaa
     1501 gcaagaactt ctcgaaaaat atggctctgt actagcactc aatgatgtga acatcgatat
     1561 tcctgccagg ggtgtccagg tcattatggg actgtctgga tctggaaaat cgacactgat
     1621 ccggcatatc aacaggctca ttgagcccac atctggggag attattgtag atggtcagaa
     1681 tgtactggat atgagtaaaa cagagcttcg tgaatttagg agacacactg catccatggt
     1741 ttttcagaga tttgctcttt taccccatca tactatcatg gaaaatgtca gttacggttt
     1801 gacaatacaa aacgtgtcaa agcaggacgc ttcagaaaga agtcagcaat ggatcgacag
     1861 ggttggtctg gcagggtacg aaaactatta tccaaatcaa ctttccggag gaatgcaaca
     1921 gcgtgttggc cttgcgcgag cacttgctac tgatgctgaa atacttctta tggatgaagc
     1981 tttttctgct ctggatcctt taattagaac agacatgcag gacgtgctac tgggtctgca
     2041 ggaagaactt cataaaacaa ttttatttat tactcacgat cttgatgagg cactacgaat
     2101 aggagaaaat attgcgatcc tccgtgatgg acttgtagtt caaaaaggaa caccgcaaga
     2161 gattatctta aatcccgtag ataagtacgt gaccgatttc ataaaggaca ttaatcgagg
     2221 tagagtgctg cgtgtttcct ccattatgga gagtaagaaa ttaaagaacg gacccgagat
     2281 cgagaagaat atgattattg aagatgcgct ccagattatt tcaaatgctg aggtaagcga
     2341 agcaatcgtt acagaaaatg gcaaaaccat tggttcagtt aaacttggca ggatgattac
     2401 cgctatcgca aggccaagcg aaaaaacagg gcagatcacc aattaccgct agattttcaa
     2461 cctttctttg aaaaaagatg aaagatcctc tttttttgat tgactcttta cagtactaga
     2521 actggccgtt tccaaacaga ctttctcgaa tgagttggca agtcctttcc acagagtatc
     2581 cgtttgaata agtcgcttcc ggaagatcgt aaagtggaag tgtagcgagt gccggaacgt
     2641 ccggtgcaag taatgtcatt ttcaatgcat cgagattcca ggcttttctt tacgtcttgc
     2701 gacaaatgct tccaattcct caacccgtga ttcttccata ggcggttgct gataatcctc
     2761 aagtgctcgt ttccaaatgt ggttggcacg ttgagtagtg tctaaaccac cgtcttctgt
     2821 ccattgacca aagttgttcc aatcggatag tatgggcgga taaaatgccg tttcataacg
     2881 gtctaaagta tgttgtgtac caaaaaaatg acctccggga ccaacatcgg caatggcctc
     2941 aaaagccatt tcgtcttcgg aaaaagaaag ttcatccaga aaagcagaca tcatctgcaa
     3001 taattcaaca tcgacaataa ctttttcaaa tgaagcgcaa agtccgcctt ccaaccagcc
     3061 tgccgcatgt ttgacaaaat tgcaatggct catgatcgtt ggccaaagtg ccatcatcga
     3121 ctcataaccg gcttgagtat cgaccgtatt acaggcattg acattggagg cacgataggg
     3181 caggccgtaa cgtcgcgcca gttgtccacc gatcaaagtc gttttggcca tttccggagt
     3241 tccaaaagcc ggtgcgccgg ttttcatatc gacgtttgaa gtgaatccgc cgtaaatcac
     3301 cggcgaaccc ggagcctcca tttgcgtaaa agccagagag gccagagctt ccgcattttg
     3361 caacgataaa gcccccgcca aagttatcgg agccattgcg ccggaaagcg taaacggtgt
     3421 cacacaaacg gattgcccgt tgcgaatcat ttccaacatt ccctgaatca ttactccatc
     3481 gagccgcaac ggtgaagaag tgttgataat ggaaatgaga ctgggacgtg ttttgagttc
     3541 ttcccgtgtg gttcccagtc caatgcaaag catatcgatg gtgtccgaaa tgcgttgttt
     3601 ccccagagaa taagcgtgaa aaactttatc cgtcagtgtc aaatgtgtca gggcgcaatc
     3661 caaatgtctt gtatttgccg gcaaatcaat gggttccacc ggataacctc cggtcatgtg
     3721 aatgatgttg aaatattgcg ccagtcgaat caaattttgg taatccttaa aatttccggg
     3781 gcgtcttccg ttgtccgtat cggaacaatt tggcgtactt cccaccattg aaaacaaagt
     3841 ccaattcttg ccgacttcca gattgtgcgc cggatttcgt gcgtggagcg tgaattgtga
     3901 cggtgctttg gcgatatatt cctcaatcaa ttcaggggaa aacttaacca tttgtgaaga
     3961 acggtccact tctgcaccgt gttctttaag aatatccaga gcctcatcat ccaaaaaagc
     4021 aattccgagt tcccgcaaaa tgcgcaaact ggcttgatga atttcttcaa tttgatcggc
     4081 acttgcaatt tcaaatggcg agtatggatt acgaggctgt ttccatggtt gctgttgaat
     4141 accgttatta gttcgagact gcgcccgccc cttgcgtttc ctttctcttg acatatcttt
     4201 aatagtatga aatggtttga gtctttgcct gatggtaaat aaaattaaaa aattctgact
     4261 agatctgatc tcgaaggcaa agatgccaca gtaaagacct tgtattcaga cttcaaagca
     4321 tttccattgc aaatggataa cttgattgac gttcaactcc tgtttccgtc atgataattt
     4381 gctcttcaag ttttacacac tcacgtcctc cttctgatcc tattactgat tcgacacaaa
     4441 gagtcattcc gacttcaatc aagccgtcat agcctttgtc atcaaaatct tcgcagtgta
     4501 tgatattggg atattcgtcc gccaagccga ctccgtgaat caaagtggta taacgattag
     4561 cgagaaattc atccggaatc ggccagcatt tttctgaaac ttccctgaag gtcattccgg
     4621 ctttcaaaac tgaaatattg tgttcaattt gttcaaaggc tttggtgtac aaacggcgtt
     4681 gttcgttatt tggtttttct ccacaaaccc aagcacgtga catgtctgag caatacccgt
     4741 acggcccgat caaatcggta tcaaagctga ccatatcacc cttttcaatc tgacgcatcg
     4801 aacattccct gaaccatgga tttgtgcgtg gtccggaaga aagcagacga gtttctatcc
     4861 actcccctcc aagacgaatg ttcgtttcat gcagtttcgc ccatagtccg ttttcagtaa
     4921 ttcccggttc caaggcttca tgcatagaga gaactcccgc ttcacaaaca tccatcgagg
     4981 cttgcataag taccagttct tcttttgatt ttattgccct tgcggtttcc gcaatttgct
     5041 caccttcaac caattccaaa cctgtttcgc gcaaagcatc acaagccgta tgggacagaa
     5101 tgtcaatcgc cagccgacga tttccgccac catgattttt cattaaaccc tcaatttgcg
     5161 cagcaaattc ttttacaaaa tcatgacaac gttctccagc accgaaaaaa gaaaaggctg
     5221 gcagaacacg gtattcgtcg atggttggta tgccctcaga caagtaagga tgattggcat
     5281 aatcgaacag aactaccgga ccatccaggg ccacaaacac gcagcgggtt tcataatgag
     5341 aacaccaaat ctgcatattt gtcgcatcca ctgcatagcg tgtattaatc tgattgaaca
     5401 gcaataatcc ggagacatcc aattctccca tttttgtacg caaacgttgt aaacgataac
     5461 gccttactgt gtctaaatca accggtgctg tggagaattc catggcccca tgttgaatgg
     5521 cacgtccagc ttttatttct tgaactgaag ctaaaggcat aattccattt attttttagt
     5581 attggcttgg atagtttttt aaaattctga aattaaaaaa tattttattt catctcttga
     5641 tctggtttcc tgacgggaca aatcactctt aattctccac atcaagtact agtgttaaag
     5701 agttttatac ccttgcatcg ttatctccgg atggaactcg atgaataccg cacattcgat
     5761 ccgccaccca gttttgataa cggtgcagtg gttcttcgaa tttaggacac attcgtccgc
     5821 ctgtaaatgc ggtgttgctc aagcccagtt ggacccgttc gacaatgccc aaatcctgag
     5881 tgttgaccat atcccaaaat ttcaccaatt ctaaaaacat agaagtgtgc gactcgttat
     5941 ccagagtccc cggaggtgtg agtaagaagg tccgttccct ggtttggttt gcacgaatcg
     6001 ggtcggtgat catacaaaaa gcatgattcg gcagtatggt gattgtgaca ttggggaaaa
     6061 tacaaatgtg atacgctgcc tccagatgtt ctttgttcaa gccctgtacc gcaggaagat
     6121 tcagccatac cgaatccaca tcccgcgaaa caggtgtcgt cagcatgcca gtatacatcc
     6181 ctgctccctg actacggtga tggtcttcca tgcgggaaac atttgtaagt tctggatgca
     6241 caaaaggcaa gtgataatac tccatgaaat tttcctcaat caatttataa ttggaagcta
     6301 catcataatt cttctcacca aaaatctccc agttttccaa aacatgtgat tgaaattttt
     6361 gcggtatatc accaagccat tcctcaagtg gtgtcgtttg cgcactcaaa ttgacaaata
     6421 ccaaaaatcc ccaagtctgt actgaaagtg gcaagagccc atagttcttc ttatcaaaca
     6481 aagctgtatc agataaatct gtttctcctc cgtttgaagg gacagtatct actatttttt
     6541 catttcggaa catcgacgtt cccaaacact ggccatcaag tccataaacc caggcgtgat
     6601 acggacagtg gaggctttta atattacaac ttttttctaa taatttagaa ccgcgatgac
     6661 ggcacacatt gtaaaatgca cctagtttcc cagttttgtc tcgcatcact aaaatagatc
     6721 ggccagcaac ttccgtaacc agaatgtctc caggattttg aacttgttgt aaacaaccaa
     6781 cggcaaccca agccgttgcg aatatctttt cctgctctaa agcaaagaaa tcagctgccg
     6841 tgtaagcatc tggaattaaa gttgaggctt gatctacagg taggcgactt aagacgtaag
     6901 tttccggttg agtgtaaacg tgggtcggca atctttgttg actgtaatat tgcatattag
     6961 ctgtttaata ttttaaaatg gtgcatcaat agaattctga aattgtgact cataatatga
     7021 cttcagtttt tgtgcagccg aaacggtgtt gcgcatcaaa caagaaaccg tgaccggacc
     7081 aacaccacct gggaccggag taatccagcc ggcttttttt gaacaggaat caaaatcact
     7141 gtcaccaaca attctgcttt tccccttatc atcagtgact ctattaatcc cgatatctat
     7201 cagtgttgac ccggatttaa gcatatcccc agtgatgaga ttcggttttc caaccgccac
     7261 aaaaacagca tccgccctgc gtgaatgatc cgcaacatct tttgtcatgt gatgacagac
     7321 ggtcacagtg gctccttctg ccatcaatag gaaggcaatc ggtttaccga caatttccga
     7381 gtgcccaatc accacaacct ccaaaccttt taaatcctga ccggtacgtc tcaacagttc
     7441 tactgaagcc atggccgtac aggggccgag ttccatttcg ttgtaaacaa tatttccaat
     7501 cgatgcgggg tgcattccct ccacatcttt gagtggatga atagcttttt gcaaagtttt
     7561 aatggaaatg cttttgggaa caggacgctg taaaaagatg ccggtgacct ttggatcaac
     7621 attgaaattg gcaatagccg cctgcagttc attcgcggat atatcattcg gatactcttt
     7681 ttcttcaaat ataattccta ttttctctgc tttcaaacgt tgattgcgga tgtaaagatc
     7741 aacagcgggg ttttctccga ctttgattga aatcagcttt ggatcccaac cacttgtttt
     7801 taattctgat actttgctgg acacttcatc aagaatgtcc ttggcaattg ccttgccgtc
     7861 caatatgttt gcactcatga ttttactaat gctatcttgt gggcaaaaaa gaagttcttt
     7921 tcatttttaa ttattttcca gactttcggt acagatttga aataatcaca aataagtgat
     7981 ttatgtttag gagtgtcagc aattaaaata aaaccagctt tggagactgc attactggat
     8041 ttttcaagaa ccgcattgcg tatttcgtca ttctgtaaca tatgaagcac actgtcaata
     8101 actacgatat tgtacagatc aggcgcttca taattcgtaa tatcagcaat aactccatca
     8161 acagctaaat tttcactttc cgcttcttcc agcatttgtt caataccggt ttgagccgta
     8221 tccactccta aaaccgaatg tcctttgcgg gcaatgaaca aggcatctcg tccttgtccg
     8281 caaccagaat caagaacagt agcacattca tcatcataat tctcaaaaaa ctcaactatt
     8341 tccggaaacg gctcgccgca aacattgcga ctattttggt attctttctc gtaatttatc
     8401 ataaactagc cccgcatgat ctcacctttg ggatcaaaaa gcggttctgt ctgcaaagta
     8461 gccggatagc gttctccaag aatttccaca aaccagcctt gtttttcgtc agcaatttcc
     8521 ttcggcacat aacccatcgc tactgaaact cctgaattgt gagcaaagcc tccggaagtt
     8581 acccagcctt tgacctcgtt tccgaaccaa atcggttcat cgccaatcac atcagcatct
     8641 ttcgcttcga caacaaaggt gcataaacgc aaaattccgc cgtccgattt ttcctgaatg
     8701 gcggcgtctt tgccgataaa gtccgcatct ttattcagag ctacaaaggg gcttaaaccg
     8761 gcttcaagcg gcccgtaaag cggacgatat tctgtggccc atgaaccata gtttttttcc
     8821 aaacgcaaag cattcaacgc ccgaagaccg aataaacgga tgccgaattc ttcgccttcc
     8881 cggagtaaca gttccagcac atatcgttga acatctgctt ccatccacaa ttcaaaaccc
     8941 aaatctccgg tgtaactcac acgccccacc aaacaggaaa ccatcccgat attcatttca
     9001 cggatgtcca taaaacgaaa ggcatctcgg gaaacatctt catccgtaat tttggccagc
     9061 aattcacggg actttgcccc ggcaatcgat agtccgagaa tgtcagctct gtgcggaata
     9121 acctccaaat ttgcggattc cggaagatgt tgttcaaacc atcgtaaatg atattcctcc
     9181 gcaattccgg aaccagcaat aaaaaattcg tcatcatcaa gattcgccaa agtaaaatca
     9241 ccaatcaatt ttccgttttc gttgagcatt ggtgccagag tcatgcggcc gggttttgga
     9301 attcggcaag ccagaatttg atccagccaa tcagaggctc cggaaccaac tacggtatat
     9361 ttagcaaatc cggaaatctc cagcacaccg acaccttcac gcacggctcg aacttcctca
     9421 gcaacgtgtt caaaatcggt ggaacgccgc catgaaaatt tgtctgttac gccttttggg
     9481 gcaaaccaga gaggaacttc caaaccaaac gaactcccca tttgcgctcc ttcggttagc
     9541 attttgtcat aaaccggagt tgtccggagc ggtcgtccgg caggcaattc ttcgttcgga
     9601 aaacgaatcg aaaagcgacg ggaatagttt tcctgcactt tggtattggt gtaggaaagc
     9661 gtggcccaat ctccaaaacg gctgacatcc atgccccaaa catcaaaacc gggatcgccc
     9721 tcaatcatcc aatttgcgag tgccaaaccg actccgccgc cttgactgaa tccagccatc
     9781 accgcacagg cacaccaaaa tcccggaagc ccccgaaccg gtccgatcag tggattccca
     9841 tccggcgcaa aagtgaaggg tccgttgatt acttttttaa ttccggcatt ctcaaaaacc
     9901 ggaaaatgct caaaaccaac ttccagagaa ggagcaatcc gctctaaatc cggaggcaat
     9961 aagtcctgtc cgaagtccca cggagttact ttgggcgacc atggtttgca ggctttttca
    10021 taagttccca tcagcattcc ttgttgctcc tgacgcaaat aaatttcgcc gtcaaaatcg
    10081 attaccgccg gaagttccag ttcattttcc tgattaaagg ccatcacttc cggcatatcc
    10141 tcagtcaaaa aatacatgtg ttccatcgcc aaaaccggca actccaaacc aaccatacgc
    10201 ccgacttccc gcgcccaaag tcctccggca ttaacaacat gttctgcgtg gatttcctca
    10261 acccgcaccg gatgttcctg ttctccggaa tctccatttt ccaaaatcac ccgccaacca
    10321 ccttgtggat ggatttgaag atcggtaact cgtacatttt gataaatttc accaccctgt
    10381 ttttttgcgg ctttggcata agcccaagtt gttccgtaag ggtctaagtg gccttcaatt
    10441 gaatcgtgta aggctcccac aaaatgctcc ggattgatga gcggcatgat tttctgtgct
    10501 tctttgggac tgatgatttc agtctccaag ccgaggtaac gccctctcgc gtgaatgctt
    10561 ttgagccatt caaagcgttc cttagtcgca gccaacataa ttccgcccgt cacatgtttc
    10621 ccgatatctt gtccggatat ttcttgaatt tcctgataca gatcaatcgt atattgttgc
    10681 agtttggcaa cattcgggtc accgttgatg gtgtgcattc cgcctgcggc gtgccatgtg
    10741 gagccggatg tcaattccga gcgttccaac aaaactgcgt ctttccagtc taatttggtc
    10801 aagtgatata aaacactgca tccgaccacg ccgccgccga tgacaactac ttgtgtgtgt
    10861 gatttcatta atactttaac atagtgagtt tacgggattg tgcttccaga aatatacttt
    10921 tgttttatca aaaataaaag taaattatat aaaaattact ggtaagattt tatatgaagg
    10981 cagtcaatgg tttggattaa ctcaatgtat ttgacgcagt gatccaagga aactagaaca
    11041 gtttgtcatt catctcctta taaaaacagg tcgtccggtt ggtcctgtga cacaacaaat
    11101 gtggaattat tttttgcaaa tagcccctga atagccggtt gtttccggtt tcaggctatt
    11161 ctcctctgat tgaacaaaca atgaggacag ttcgtggact gtagattccc agtgaaagta
    11221 ctgtgtatgt actttgcatg tatgtgctgt aaatgagtat ctcataaact ctaccctggg
    11281 ggcagaggtc ataaacttcg tatttttttt cacctactaa accccaaaac caataatttt
    11341 cttcatgggt tacagtagtt tttgaaggaa attctgtagg atagcgtacc tgaacttggc
    11401 atcctccaat gaatagagcg aaaattaaaa tcagacagta ataacggagc atagtactaa
    11461 ttttggaaaa atattgtctt gccttgcgtt tcaaaaattt cgaatttaca atcttagttt
    11521 agactttgaa aaagtgtaat ccagtaaagt tttaccagaa taaagaaatt tggctgaaaa
    11581 gcatccgggt tccagaattt tgggcgtaaa tagccggtcg ccttcccatg aggcaagttc
    11641 agtcaggttg tctttgggaa tccaatcaag ttgaccctcc ggacaatctt ctgttagagt
    11701 accagaatag cagtttgagt aaaaaacaaa aacatgccac tcatcaccaa aaggtgagtc
    11761 gccctgatca ggaaaagaaa gtactgcttt tagttccgga tcttcaattt tcagtccggt
    11821 ttcttccagc atttcacgga ctgcagtttc aaacggagct tcattaggtt caactttccc
    11881 tccgggagct aaccagaggc ccttatgttc gtcttctttt tcacgatgaa tcatcagaac
    11941 atgttcatca ttattttcaa gataaaccag aacacctaat ttcacaactt tactccaaaa
    12001 atttatgcct cagtcgaaaa aagaattaaa tgcgattctg aaacgcattt cagttgaaca
    12061 agacaaacga ctgaacccgg aagaagatga aaaaacagtg gtgttgccgt acgaattggg
    12121 ttttcaggct ccaattattc gcggtcgtat gccgggactg gaagcagttt atgaccgttt
    12181 tgctagaatt tatgctcaaa ctttttcaca tcatgtgaga cgtccggtta ctatgacacg
    12241 gcgtgctacg gagttagtca gttttcgcga ttatcttgct gccatttcta ctccggcttc
    12301 actgacagtt tttggcatga atccgcttca tggacacgcc ctgtttgttt ttgaacatca
    12361 tctggtgtac atactgattg atatgctttt cggaggaaac ggctggtttg aaaatccacc
    12421 tccaaaacgt gagcttaccg gtatcgaaat taaaatgctg tccaaagtcg tccacagtgc
    12481 tcttgaagat ctaggtaaag cctggaagag attgactccg ctggaacctt attttgaccg
    12541 catggaagtc aattctaaat ttacagctat tgttcctccg gatgaggttg tggcaagtac
    12601 tactttcgat gtagaaatca accgtgtccc ttacacaatg agcctttgtc tgccttattc
    12661 aatgcttgac ccagtacgta cccaacttga ggcaggaaaa acaagctatg atgaagaggt
    12721 caatgcagtt aatgtaagtc gtcttgaaga aaatttgctt aattcaaaag ttggtgtaaa
    12781 ggttgacctg ggggaagccc gcatgtccct tcggagtttt ctaactttga aagagggtga
    12841 tagaattctc ttaaatcagg atcaaaataa accgcttaaa gttctggtac aggacaagct
    12901 gaaatatttg gctactcagg gtgcttacaa agggaaaaat gcagttcaaa taaccaagct
    12961 aatagagcct cctccacgct ttagtgactt actggatcaa ccagcaaaag atactgcgga
    13021 agattcatga tatatctaaa ctgttactga ttgactcgac tgcgcaaatc tttgcctgtc
    13081 cggaacactg gaagttgctt tggtttgacc tccacagttt ctcctgtctt aggattcctt
    13141 ccaatatatc ctttatattt tttcagtgta aaagaaccaa atccgcggat ttctacttta
    13201 tcaccggcac ttagtgcagc tttgatacgg tcaaaaaaga tacgaaccat tttgtccgtt
    13261 aattttggat tgtatcctgt tttagccgct aactcttcta ttaattctga tttgttcata
    13321 actcatggta tttaaggtta gttgattaaa atctgaaaac tcttggattt caatcggatt
    13381 cttctttgaa aaaaacttga tgatgtaaag ttagggtaaa atgaaaaaat atcaagtgat
    13441 ttagtaaatt atttttatat tgattgaatt tgcagttcac gacgataatg gtcgaagaga
    13501 gttgatttag aaagcatacc taaaaattga tcaccttcta ctacaggtaa tgaccaggct
    13561 ttagaggttt caaacttgtc taaggcagca ctgattggct catttaggct gattaagggc
    13621 agattacgca tgactgatcc cattgtcact aaatcataaa gacttctgtc aaaaagatga
    13681 ggacggatgt catcaagaaa aacaactccc acacatttat tttctttatt cagaacagga
    13741 aaatagtttc gacgtgcctt cttgaattcc tcaataaaat ttctcaaaag cagatcttca
    13801 cgaatagtta tgaaatcctt gtccaaaata tctccaatat tcagatgatg cagcaagtaa
    13861 cgatcagttc cacgcttgat caaactaccc tgtttaatca aatcctgggt atagactgaa
    13921 ccgatctcga aaaatgaact tacaaccatt gcactgcttg cagttaacat cagcggcaaa
    13981 atcattgaat aacctcgagt tacttccata acaaggaaaa tccccgtcag tggtccgtgc
    14041 atgacacctg caaccattcc tgccatacct gctagagcaa aagcagcatc ttcagcaacc
    14101 ccgtccagtg ggagtgaatg catgatcatt ccaaaagcca aacccacacc actgccaatc
    14161 acaaggctgg gcgcaaaaac tcctccgatg ccgccactac cgagtgtcat cccgcaggca
    14221 agaaatttga gcagtacaaa aagcagaaca aaagcaagtt ccggacgtat tgaatcagtc
    14281 agaaagactt gagtagctga gtatccttct gacaacactt caggcataaa aaaacctaat
    14341 gttcctacaa acagcccacc aattgcggca cgtatccaat gatgaagaga gacttttgta
    14401 aaaaattttt cccataacaa caaacttcgg gcaaacaata tggagatgac tcctgtcaag
    14461 gccccaagta caaagcaggc aagtaacgag gtgagggaaa aaccagctat ttcctgaaag
    14521 aaagcaatct tatttccagt tatccagcga ctgagttctg ttgcagaaac agctgcaatg
    14581 atcgtgggta aaatagttaa atacgtccat tctcctataa ttatttctaa tgaaaaaata
    14641 agacctgtaa gtggggcatt gaaaattcct gcaactgcac ctgctgctcc gtaaccaaca
    14701 agaagttttt tgtgacgctc attcatgtta agccagcgtc ccagtgttga acccagtgct
    14761 gcaccaatac tcactattgg accttccaaa ccggttgaac ctccactacc tactgtcaga
    14821 aagcttccga agaatcgcga gaaaatcatg cgtctcctga gaccttctga tcctattgct
    14881 atggccttta ttacatctga cactccatgt cctgcagagt catgcatgag agatcgcacc
    14941 aaaaatacag caattccggc tcccagagca ggaaaaaata tggctcgcca acctaagtct
    15001 aagtcgctta tgaagagtct tagagaatgt acgctccagt taagtccgac ggcaagaact
    15061 ccgcaaccca gaccgattgc caatgctgca gtcatcatca ccacagcttc cctggcagaa
    15121 acagcctggt aacgtgaaag tagatcggtg tcagatccag ccaaaccgcg cgaaaggaat
    15181 ttgttaagct ttctctgcca gtgttttttg aaattgttca actagattat ctaatgaagg
    15241 tgaattgttg agaaagtcaa tcatctgcga ttgacttaga atacgggcaa gttgagaaat
    15301 aagttgaaga tggtgaaaag aatcaacact caaaagtaca aatacaacaa aaacaggtaa
    15361 accgtccggt gccataaaat ccaatgagtt ttccaaaaaa aatgtaccta ccatggattt
    15421 atttatttgt ttacctagtg gtgttttaca atgcggaatc gcaattccgt taccaattcc
    15481 ggttgaagaa agatgttccc tttgcaaaag caattcggct aatgagtctc caataggttg
    15541 aggcaaatcc atttgtactg aaacttgctg aaaaagtgat tctattgaac ttgatggaac
    15601 agcatgaaaa acatttcctg ctttgagagc ttcaatcagg cgtgactgag aaacttgttc
    15661 tttgctcttg cgggatcttc ttttgtgttc ttcaaggtgt atgtgttttg aatcagccca
    15721 ggaaattaga gtagattgat taaagtaata tttcccgcga cgaatgatgc agggaatatc
    15781 accctgacgt atccaacgat aaatagttgc ttcaggaaca tgcagcaact gaacgacatc
    15841 tgaaacaacc aaccatttag acatgtataa ttgaaagaca aaaatattta atgaaaaagg
    15901 gttataatat ttggagtaac cagtttaaca gtattaactg tctttgtgaa gaagtgtaat
    15961 ctatataaat taaattatta agagttgaaa attggtgcat tatttatgaa aatcaattac
    16021 aattttctaa caaaaagtta cttagaaaga aattcaaagg caattgaaag gttaaattaa
    16081 taaaaaagac actatttcaa atccaatgtc ttgatattag cctaaaaact ttagataaag
    16141 acttcttgtg gctgggggtg cgcaatcgaa aatttcgaac cgtgtcagat cctagcggaa
    16201 gcagcactaa gatttcgtag atgtgccgca ttcctcagcc tctcttcgta aatattggaa
    16261 ttcctctcat gtcttatgtc gttctggcgc gcaagttacg cccttcacgt tttgacgatc
    16321 tgattgggca agaaactgca gcccagactt tacgcaatgc agttctttca gacagagttg
    16381 ctcatgcctt tttatttact ggatcacgag gagttggaaa aaccagtgca gccagaattt
    16441 taacaagggc tgtcaactgc ttgaatcccg acaatgggga cccgtgcaat agatgtgaaa
    16501 actgtactga aatcaatcaa aacgcatctg cagatgttat cgagatagat gctgcctcca
    16561 accgtggaat tgaaaatatt cgtgagttgc gtgaaaacgt aaattatgta ccggcaaaat
    16621 gccgctacaa aatatatatc attgatgagg cgcacatgct cactctcgag tcttttaacg
    16681 cgcttcttaa aacactggaa gaaccacctc cgtatgtgaa atttattttc gcaaccaccg
    16741 atccccataa aattcctccg actattattt cgcgttgtca acgctatgat tttttaagaa
    16801 tttcaataaa aaaaatggca gattttctgg aaacagttgt caagaatgaa aatctcgagt
    16861 tgtcacgcaa ggctattgaa atgatctgca ggaattcagt aggcggaatg agggatgcat
    16921 tgactgcaat tgatcaaatt ctaagtttta cgggaacatc tgcgactgat cagaaagtag
    16981 cccagatttt ggggatactg gacagtgaat ccaggtttgc atttattgag gctcttctgg
    17041 aaaaaaagga tggtgaggcc ataagttatt ttcagaaact tcaagaacat ggacatgatg
    17101 taaatgatgt attatctgat ttgttacaaa ccattaaaac catcgcactt gtgcggacac
    17161 ttgggatgaa tcaagctctt tttcaagata ttcctgctga tgatctggaa acattttcta
    17221 ctttaagcaa aattgtcagt gccgatgagt tacaacagat ttttcatatt ctgttggagt
    17281 tggaagaaca gatgaagcgt agtgcacata ctaagatttg ttttgaaatg gctattctcc
    17341 agattgcatc cattgagcct cttgtcggcc ttccggagat tatcagtgaa atacaaaata
    17401 tacgtaaaag taagtctgcc ggaaactcaa atacagaaaa tacagattca gtaattgaag
    17461 gtccagcgga actctcaggg ccggaaaaag ataattccgg agcaaatcag cagcactcat
    17521 ttttaaaaat gtccggagcc cataaaccat taacggacgc aactgtaatt aaaaacattt
    17581 tgaagtcaga caagacggag tcagctgctg atgaacctgc caagttgaat gaaagttctc
    17641 ctgatccaaa aatgactgaa aagcagcatt ctgttacaaa tcaagataat tattctcatg
    17701 ttgatgaaga aaaaccatat gttgcagaaa cagaagtatc ccaggtaaaa gctgaacaaa
    17761 ctgaaaatgc agataaggaa atgtcaccgg aagctcagaa taatattaat caaaatcaag
    17821 aaattgtaaa acttgctgcc aaaaaaaaca ttgaagaaac ttcaatggaa cctccggaag
    17881 aatggtgtaa cttttcagaa gaagttcaaa aactgtcatc aaagctggcc agttttgttc
    17941 gcaatgcggt ccctcataat ttgacaggat cacaattaat actttctttc aaaagcggaa
    18001 attatgcatc tatgctgact gaagataaca gagagttact ggaaaaaatt gcttccgact
    18061 ggactggcca ttcagttcac gttagttgtg aaattactcc aatatctgca tctcagctaa
    18121 ctatagtaga acatgaacaa atacttttag agagaaaaaa tgaacttaag cgaaagaatg
    18181 caagagcatt tccgcaggtc aaagatattc tttctgtgtt tacaaacagc agagttacaa
    18241 atatagaatt gtctgatgca taaagataat ttgaaccaca ataatttaaa atagattaaa
    18301 ctttaaacta agggataaaa ttatgattga tatgaatgaa atgatgaaaa aggctcagga
    18361 actttctgag actatgaaac agcaacagga ggaacttgcc aaacagatta tttctgtttc
    18421 tgttggtgga gagatggtga agatgacttt caatggaaag caggaagcaa taagcattga
    18481 aattgatccg gaagttgtag atgcagatga cattgagact ctacaggatt taatcctttc
    18541 tgcagtcaat gaaggactca gacaaagcca gcaaaatatg cagaactctc ttgggcagca
    18601 aataggaaaa atgacaggag gactggattt atcaagtatt ctcaatccaa aatcataatc
    18661 aaatattgtc tgaacgaaaa ttcaaacagc cggacttttt tccagtttta agtaggtttg
    18721 taatagccaa agatttagct gtcagttttc agctgtcagc tcaggttgaa atataatagc
    18781 aaataagctg actgctgaaa gattttttgt taaatctata ttgttgaaat cacagataaa
    18841 aaaggttgtt attattgctt ataattttgt tattacacta tttgttaatt aaaaatgcga
    18901 atctggcgtc tgatcgagga cattccccgt accggcagtt tcaatatggc agcagatcag
    18961 gtgctgttag aaaattattc tcaggatgct aatccagtat ttagaattta tgactgggaa
    19021 tgtactaccc tttcactggg gcgtaatgaa atattggaca atctaattga tttggatgga
    19081 tgcgggagac ttgaaatccc aattgtccgc cgttcaactg gcggaaaagc tgttttacat
    19141 ggttttgacc tgacttactc cattgttggc ggagtcctgg atcagcattt ttccggaggt
    19201 gtattagaca actaccgcta tctggcaaaa ggattttatg ccttttttaa aaagttggga
    19261 cttaatcctg tattgaaagc catagaccgt caaaagaaaa aatctgactc gcatatctgc
    19321 tttgcagatc ctgctgtata tgaaatattg gttgaaggta gaaaaattat tggcaatgca
    19381 caacgtgtaa gaaatttccg cagttctgat tcatcttcat gcagagtgtt tcttcagcat
    19441 ggttctattc ctctcaaaga ttctataccc cagataatcc aaatattccc acatgcagca
    19501 gaggatgatc tgcgtagaga aatgcactca cttgaaactg cgggggttta tccaggactt
    19561 gcccaaaaaa aattaagaca gatgtttctg gactgcctgc aggaaacttt tcaattaaaa
    19621 tggaaacgtc agttctggag tgttgaagaa ttgaaaatga tttcggaacg tgaaaattct
    19681 tttcaaaatc ttaatattaa ttagtgttgt aattctaacg gagtcactga cagctaaatt
    19741 cagcagaagt cgttataaat ttggattatt gacttcaatt caaagactgg gaacaatttc
    19801 aactgttatc tgtaacacca ttttctttca tagttaaaac tattttaatc aaaagtaaaa
    19861 tccatggtgc agcatgcatt attaaatcga accaatcgat tggcctgaca aggggtcccg
    19921 aagaaaacat ttttaatttt tcccacacgt gggggatagg gaaaaatgga gcaagtccca
    19981 aagtaagaca aaacaggatt atcatcgacc atggaagttt caccactaat tcacttatag
    20041 ccttcagcat aggtgttttt agttacaaaa gattgatatc aggcttaatt taagtatatt
    20101 aagcatatgg tatgacaacc ttaaaggaca gatacatttt ctttttagag ccatttgtac
    20161 atgagcaatg catccacata accaagtttt ggatgcagga atgctttggt aattcttccg
    20221 acagtttcaa atcccaatct ggtccataag cggattgcct gctcattact gactactacg
    20281 aggttgaatt gcattgcttt gtagtccagc tctttggctg ctttttgtga gtgttcacac
    20341 atcagtgtcg ccaatccttt ccctctggcg tcgggcgaaa ccatataacc gcagttgcaa
    20401 acatgactgc cttggccggg attatttggt tttagatagt aagtcccaaa aatgtgctgc
    20461 tgttcaacgc agacaaaggt tttgtgtggc tgctccatcc aaatttgaaa agcttcttct
    20521 ttacttgtct ctacgggata agcataggtt tcacctgcag aaacaatatg ctgaaaaata
    20581 atccaaatct gttcaaagtc ctcttttaca gcttcacgaa ttttcatgaa tgttgtccgt
    20641 tttaactcaa aagtgtaaat gctcgccaga gcatccctgc agccaataaa atcagcagta
    20701 cacccattat taggtctata cgtttagcat tgcgctgcag cgcttttaca agtcctccgg
    20761 aggaagcaag aaatgcaaca agacagtacc agcttgtatc aacacccccc acagttgaga
    20821 ccattaccaa ctgttcagta aagtcagcat caggctgtac gaattgactg aaaagtgcca
    20881 ataaccagac tgcaatttta ggattcagaa aagcaatcaa aaaaccttca ctgaaaccac
    20941 ggtacccggt tattgttgat tgttttagtt ccccattatc ctgctcgcca ccaataccgg
    21001 aaataatcat tttgccgcca agcaatatta aaaacaatga tccaattatt tgggctatca
    21061 ggaaaaaatt aggaacagtc tttaacagag caaccaggcc agttatcgca accacggaat
    21121 agaatgtaat acccagaccg tgaccgactc cactcattaa accctgagtc ctacctccgg
    21181 agatagtatt tcgtaaaatt accgcaagac ttgggccggg tgacatagca cccatgacac
    21241 agattaaagc taactggctc cattgtgtaa aatccatttg tttactctat attttaccgt
    21301 acaaatttgt cattgaaaca ttaaattcgt aattactggt taagttttcc gagtagcaga
    21361 caattgccat acagcaaact gcttttaact gcaggcttaa aatctgttct taacttagtc
    21421 atgttttaca tcaaatgatg aggatctaca tcaatctgaa tcaactcatc acgtctcgtt
    21481 gaaggtggtg tttctaaaac ctgttttaac aaacgcaaaa tgggtcgtac attatctgct
    21541 ttcagtaaaa gctgccagcg aaaacggttg cgcaattttt tcatcggtgc ttctatcggt
    21601 ccaatgactt gtactgaatc aatcggtttg atctcactgg taaactgggc agtgtttgaa
    21661 tagtttttgt tagaagaaaa cttttgaatc ttttcggcta tttcccaaat caaattttca
    21721 gctcgttttt cattaggact gctgcagaca accaaagtta gtgaatgaaa aggcggcatc
    21781 cggagattct gacgtcgttc aagctccatt tcccggaatt gcctggtatc gtgttcttta
    21841 gcacaaagta aactatggtg cctgggatta tgagtttgaa tcagcacctc acctggttta
    21901 taacctcgtc cggcacgtcc agctacttgc gttaatagtt gaaatgtccg ttcacttgca
    21961 cggaaatccg gaatgtttag cgataaatca gataatatta ctcctacaag agtcacttcc
    22021 gggaagtcat ggcctttggt tactaattgt gttccaatta cgatatctac ttcgtgtctg
    22081 cggatacgat cgtgcatttt agaaagagca tgtttgccgt gcaatgtgtc ccgatccatg
    22141 cgtaaaatac gggccgcagg aaaaaacatt ttaagatttt cttcaacttg ctcggttccg
    22201 gtacctatta ttattggtgc gtgatcactt ccacattcag ggcatctctg aaccagaggt
    22261 ttaacaaagt cacattgatg acattgcacc tgatttgcta cttgatggta aaccaaactc
    22321 agactgcagt tggggcaagt gaatgtcgat tcgcattttg aacaacggac taaaggcgca
    22381 aatccgcgac gattcaaaaa aacaatactt tgctccttct tcagtaaacg taggcgcagg
    22441 gcttcaacca actcgcttga aaagtacgga cttcctttct gtccgggtac tgttttcata
    22501 tccaataacc tgacttccgg taagagtgcc tgattaatgc gtgaaggtaa gcttaaaagc
    22561 tcatatttac cgttgcttac attgtttgaa gattccagtg aaggagtggc cgaaccaagt
    22621 aaaactgttg cacctgcctc atatccgcga aaaatagcta catctcgtcc gtgataacgc
    22681 ggagtctcgc cctgtttgta ggaaggatca tgttcttcat ctatcacaat cagtcccagg
    22741 tttttcattg gagaaaagac tgcactgcgt gctccaatta caattgatgc aaatccatga
    22801 cgtacccttg accactcatc aaagcgttca ccatcatcca taccactatg taagattgct
    22861 acgtggtccc caaaacgtga acggaaccgg ttcacaagtt gtggtgtcag tgatatttca
    22921 ggtactagta tcaagcatga tttccctaat ttctgggctt ctcggactgc atgaagataa
    22981 acctctgttt ttccgcttcc tgttatacct tccagtaaat aagtacggta agttccgttt
    23041 tgcagtgaat ctgaaagctt ttcaaaaact gatttctgtt ccggagttaa ctccttaaat
    23101 ggttctattt caggaagtcc tccttccata aatcggcggt aaactctttt ttcaaaaaat
    23161 tcaatgtgac cttcttcttt tagttttttt aacgcctgtg atggagcatt gactcggttt
    23221 tgaacgtcgc tccaacatat ttcacgattt tcattcaata tttcaaatat ttgctgtctt
    23281 tttgttttac gccgagaaac ggaatttttc ggattatcag gtttgagcag acgtatccag
    23341 cgttccattt ttggctttgt cttttgccca agtaacacct gagttgattg aacatgatct
    23401 ttggaaagcc agttacgcag ttgttggtgg tcacgttcat cagggttgca ctgaagccat
    23461 tcctgttgtg tccatgttga ttgggtatct atcaggatct ggggtttttg gctcagagtg
    23521 tccaggccgg gaagtgaggt tgtttgggga gtataagttg tacgtaaacg caaggcaagt
    23581 ccacctggaa ttgcagcatt aagagtttca ccccatgcgc aaaaatagta ctcagaagtc
    23641 cattttgtga gtttcagtaa ttcttcagaa aaaagtggtt cctcgtcgac cagtgtttca
    23701 acatttttta gcctagtaat tccggaatgt tttttaacac gaacgatgac tccacccttt
    23761 ttctgtgctc caaaaggcac aagaacttgt aatccttttt ccggaactag agtaagttta
    23821 tccggccagt gataatcaaa cgattttcga attggtaaat ttagggcaac ttcaacgatc
    23881 atgtattatt tcggtattgc ctttaagcaa attagtggaa tctctccagt gctttccaac
    23941 cgataactac cgacaccaac aggtaaaaac agggcctctt cacgatttaa tttaactgga
    24001 gaaccacttt taggtataat actggcctgt ccggagatgg taattagcaa atggtatttt
    24061 ccatccagtt gatcctccca aatagtttcc ggttccaaac aaattcgatc agccgtaaat
    24121 tgcggaaaat caacaaaacg ttctatcagc aaaccggaag atttgtgtaa gcattcaagt
    24181 tcaggaggtt cataaactac cgggagcatt ttgtttaaag cgtcatcagt atcccaatga
    24241 ttttgggtga gaactttttg agcgaacatc agtattagac gctcgtagtg cggtgtttga
    24301 aattcaatga ctttaattcc atgtcgaaga gaatgcattt gaaacaccgg aaagctgatg
    24361 atgtcaccaa cttttacctg acagtcgcca acaaaatttg aagctttctg tcttagttta
    24421 agttcctgtt catgtagttc attcggaata tcttcggtag aatcatcaat ttggcgtcgt
    24481 attttttcat attcaccaat tgtttccctg aaatttttca gcagaatttc tacccatttt
    24541 gacccatgta tttcctgata atccttgatt ttctccggat gcagtcctgc cttgatgatt
    24601 cctgttcctg atggccaggc tgtttggtca atttctgtaa ccacgtaaac ttcccatttc
    24661 ttttcatgca tttcataata caaatctcca atcacatcct cagaaacagg atttagtgtt
    24721 ttgagcaaaa tcagcgattc cggatgatcg gcaagcactt gttttttaaa aatattcagt
    24781 gcataaggta actctgtttt cccatattcg tctgtaactt tcaccacacc gcgtttttct
    24841 actcctgtgt accatccttc atgtccccat ggttttggga tattgaggac ttcgaggcgt
    24901 aaagactgtt ccggattgac tattacggca tgtctgtttt ttgccacaga ttcgagtgga
    24961 gcatcaggat tttgtacgat tattcccttg agagtttccg gatcgagctt gtttttccag
    25021 acactacctt gcttttttaa tgttgcgttt ttattgttgt gattcgattt ttgaagtttg
    25081 atttcaatca attcctgaaa ggaaacctgt tcagagtttt ctgtccatgt ttctaaacgt
    25141 tttcgctcga ttaaaagtag gaaaaattga actacctgaa ggtccatttc agaaagtaag
    25201 taatctgcga ttttgcctcc gtctggtatt tcaacctcag taaagtcatg caaattgagg
    25261 tgtgcaaacc aaattgatga tcctgactca gactcaagct gctgagaaat ttctaaacag
    25321 gaaagcaagt attttatata aaaatctcgt gaagtcatgg attaaggatt aagcagttct
    25381 ggacaaatcc tggcattatt aagtgcatca gccgttgcag gtgagatagg actcagtaat
    25441 tgcgtcagtg tgattatttc ctgttcaggt atggcataag gtgttacaaa ctcaacagaa
    25501 aatgcctttg attcagttga actgggattt agattattag aaacggtttt gactaaattt
    25561 tcagcagcag caagatttgg gttaagcaga gttatattgc agaatccttc ttcattaaaa
    25621 gccttttgga aaaggtcttg acgataacca taatgagtac aagcaagaaa aatcagcaaa
    25681 gtatctatgt atttttcagg caatttcagc atggcttttt gaacccagtg ccggatacgt
    25741 tcttctacaa atgagctatc cggatcatta gaaatttggc ttgccaaatc cggacaaccc
    25801 tgggaaatga tccgtatttc ctgaacaccc ttcttaaaca gttcgttttg aaaagtttcg
    25861 gtattaatcg ttgtgggagt tccaaatatg atagccgatg attgagcatc attgagcaga
    25921 cttgataaaa gcatcttgac accaatttga agaatccctt caattttaac agttttttca
    25981 gaaggaatct ccaagtcatc caatagtaca gacagcgttc cacaagcgac aaaaatgtga
    26041 tgcggagcaa aaatcttttc agtgttccgg aggattattt caaacgtttt aagctgttcc
    26101 gctcttccgg acatggaatt gtatccacga ttatttgaag gaacagcgtt aatgtaagtg
    26161 atttgtaaat cgtcagcggg ataatttgga tggtttgccc ctgcaggttc tgataatagt
    26221 tgcagaagct tggcacaaac agacagacct ccaagtcctg agtctgtgat aacaagtttc
    26281 atgaagatat tttattggca ggtgatgttg atcagaaata atgcatctaa ccttatcctg
    26341 agacttggcc aaattcaaac taatagtgtt tacagtatga atattggagt agaattactt
    26401 agggctcact gaaaaagttt tacctttgtc atcccagact tgatccggga cccagagtca
    26461 ccgttcagct tgagtacaag atctgaaaca tttctcctgg attccgggtc tatgctacgc
    26521 accgcccgga atgacaaagg agaagaatcg aaagaagcat aactattgat cttgttaggc
    26581 cacctttttg gaaaaggaac ttaaacaact gaagagcaga gtgtttagtg cttttttctg
    26641 gaaataccct gcagttgtct
//
LOCUS       DTVD01000137            2036 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_24467_length_2036_cov_266.516406, whole genome
            shotgun sequence.
ACCESSION   DTVD01000137 DTVD01000000
VERSION     DTVD01000137.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24467_length_2036_cov_266.516406"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="EYO68_04850"
     CDS             complement(<1..551)
                     /locus_tag="EYO68_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07169.1"
                     /translation="MFVRIFTTVNQFPKTILLVVLALSAFFFVQARDGLFDSQTGRLR
                     INSTVEPFIERDSGAYQQFLDARKAFGSEEVVVIALHNTEKKPVGLEFILTLAHLKSD
                     IETTVPGITKVFSMLDIPQASGECTGKSYFHQMGIGSVCFSVLEKYEQDISCLNSTAS
                     SSTQDTEVNLEESLEGGLEESLE"
     gene            complement(559..2025)
                     /locus_tag="EYO68_04855"
     CDS             complement(559..2025)
                     /locus_tag="EYO68_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07170.1"
                     /translation="MDEDPLAESGSDLDMDEDPLAEESSQIGQEETPNIDESEIQESE
                     IQVAEQEEYQPKVTFSHEVKTLLGGTETKGLSVLEPLLSEIKELRFVSTYEQSVKVQT
                     SPRMYNYFRLSVSFSQNYELNKKRIFDGFASLREIYSNYRAGNHQIRYGTQIFGLGKV
                     DLDKVIDVLHMNNIMGLYTFDPDDSKDSIPSIRYNWFRGAHTATIYVSPIRQQTFGMR
                     FTEFREGVEKKEKDEEESKASFLRDYFGLQYQWTGDVFDTRFGFFHWFDANPHIKLEY
                     QGAADNESTSLQGTFEKMLGNYNEKETRTDFMTFELDAVWSDMVWKLESGLFKKRNLY
                     SFEITEGKNIHLSTVSAPHFAMATSFERTFPYFYWLMIYSHRKSFDVPENSHIFLYEN
                     ESVLVPQKRDVIRNQVSGVAVLKTPDNSLRITLLNYQTWPFVQKGFASLFTWEQYKQN
                     MELELKFFRLKTEKQKMMKNEIETNQVFLTYTQKFTAN"
BASE COUNT          581 a          436 c          378 g          641 t
ORIGIN      
        1 ctttccaggc tctcctctaa tcctccttcc agactttcct ctagattaac ctctgtatcc
       61 tgtgttgaag atgaagccgt actattcagg caggaaatgt cttgttcata tttttccaaa
      121 acagaaaaac aaacagagcc aatacccatt tgatgaaaat acgattttcc tgtacattca
      181 cctgaagctt gtggaatatc cagcatactg aagacttttg taatgccggg aacggtagtt
      241 tcaatgtctg atttcaagtg agccagcgtc agaatgaatt caagccctac cggctttttt
      301 tctgtattgt gcagggcaat cactaccact tcttcactgc caaatgcttt tctggcatca
      361 aggaattgct ggtaggctcc tgaatcccgc tcaataaatg gttcaactgt gctgttgatg
      421 cgcaatctcc ctgtttgaga atcaaacaat ccgtcccttg cctgaacaaa aaagaaggca
      481 ctcagggcca gaactaccaa caggattgtt ttggggaatt gatttacagt agtgaaaatg
      541 cggacaaaca tagaggcttt agtttgcagt aaatttttgg gtgtatgtca aaaatacctg
      601 atttgtttca atttcatttt tcatcatttt ttgcttttct gttttcagac ggaaaaactt
      661 tagttcaagt tccatgtttt gtttgtattg ctcccaggtg aatagtgagg caaaaccttt
      721 ttgaacaaat ggccatgtct gataattcag cagtgtaatt cgcagtgagt tgtccggtgt
      781 tttcaggact gcaacaccag acacctgatt tcgaatgacg tccctttttt ggggaacaag
      841 aactgattcg ttttcataca aaaaaatatg tgagttttcc ggcacatcaa acgacttgcg
      901 atgtgaatat atcatcagcc aataaaaata tggaaaagtc ctttcaaacg aagtagccat
      961 ggcaaaatga ggagcgctga cggttgaaag gtgaatattt ttcccctctg taatttcaaa
     1021 ggaatacaag tttcttttct taaataaacc tgattccaat ttccatacca tatcgctcca
     1081 tactgcatcc agttcaaaag tcatgaaatc agtacgagtt tctttctcat tgtagttacc
     1141 cagcattttt tcaaaagtac cctgcagtga agttgactca ttgtctgctg ccccctgata
     1201 ttcaagctta atatgcggat ttgcatcgaa ccagtgaaag aagccaaacc ttgtatcaaa
     1261 aacatcacct gtccattgat attgcaaacc aaaataatca cgtagaaatg acgctttgct
     1321 ttcctcttca tctttttctt tcttttctac tccttcacga aattctgtaa acctcatgcc
     1381 aaacgtttgc tgccgaattg gagatacgta aatcgtagct gtgtgtgctc ctcgaaacca
     1441 attgtatcgg atagaaggaa tggaatcttt ggaatcgtca gggtcaaaag tgtaaagccc
     1501 catgatgttg ttcatatgaa gaacatcaat aactttatct aaatcaactt tccccagacc
     1561 aaaaatctgt gtcccataac gtatctgatg attgcctgcg cggtaatttg aatatatttc
     1621 tcttaaactg gcaaagccgt caaaaatacg ttttttattc aactcatagt tttgcgaaaa
     1681 actgacagaa aggcggaaat aattatacat ccggggagat gtttgtacct ttacagactg
     1741 ctcataggta gaaacaaaac ggagttcttt aatttcgctc agcaacggtt ccaatactga
     1801 cagaccctta gtttcagtgc cgcctaacaa agtttttact tcgtgactga aggtgacttt
     1861 tggctgatat tcttcctgtt cagctacttg aatttcgctc tcctgtattt cagattcatc
     1921 aatatttgga gtttcttcct gtcctatctg cgatgattct tcagcaagtg ggtcttcgtc
     1981 catatcaaga tccgaaccag attcggcaag ggggtcttcg tccataccaa gatccg
//
LOCUS       DTVD01000138            2031 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_24569_length_2031_cov_258.860324, whole genome
            shotgun sequence.
ACCESSION   DTVD01000138 DTVD01000000
VERSION     DTVD01000138.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24569_length_2031_cov_258.860324"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="EYO68_04860"
     CDS             <1..191
                     /locus_tag="EYO68_04860"
                     /inference="COORDINATES: protein motif:HMM:PF07947.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HIC07171.1"
                     /translation="ILVILIMSGLARERHLQLELPQTLFAACGAILFLISDALLAWNR
                     FRMKFKSAQFLILDTLTM"
     gene            complement(420..923)
                     /locus_tag="EYO68_04865"
     CDS             complement(420..923)
                     /locus_tag="EYO68_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07172.1"
                     /translation="MKYLFIICILFWNLTGITVAAPDKAQVMELLEGRHWKLDVESFQ
                     LLGDDTDKVLIEIGGDTSLINYLRFRALDALSLFPTENTASFLELYAEKSFAPLARRG
                     FEALKNGFYKTQPQRVKRLAARLLKHPNAQVRISAARFMRSVDAPRFERFLKSESDSW
                     VRKEAQK"
     gene            complement(969..>2031)
                     /locus_tag="EYO68_04870"
     CDS             complement(969..>2031)
                     /locus_tag="EYO68_04870"
                     /inference="COORDINATES: protein motif:HMM:PF13582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07173.1"
                     /translation="EELSGVSSAKTNIDGLNTAISEITQTVGSDGLLAGFFVVPEDGV
                     SFLLSIFLGSNYNIKFYSLTDPDGTDILSASSTPNLYNEASGRLGSSGYANVLVPQSP
                     DFTAKAGTWTFKAKNNDRVKLTLRSGTVSASTTIVVQPYITGTTRSAGDLSAALSVMS
                     SIYSANGITLTINDTITISDTQYAEVSGTFTNSTTSALVSQGVTEGVNLFFIEDYSDS
                     EHLGNAAGIPGSMGIANSWNGVLISLTAHATGSTLVAQLLGETAAHEMGHQLGLFHTT
                     ESGGTVFDILTDTAECLNSTKDFDRNGKMSAEECEGYGGENLMFWTAWTTSSRSAGKK
                     QETLSSHQQHVLKYSPIAK"
BASE COUNT          551 a          440 c          432 g          608 t
ORIGIN      
        1 acattctggt aatccttatt atgagcggtc tggccaggga gcgtcatctg cagttggaac
       61 ttccgcaaac attatttgct gcgtgcggcg cgattttatt cttaatttca gacgcactgc
      121 tggcctggaa tcgatttcgg atgaaattca aatccgcgca atttttaatc ttggatacac
      181 ttactatgta gcccagtggg cacttgcaat gtctttaggc caaccgaatg tttactattt
      241 gagttgatca tttataacca tgcaaaatct gttaaaaaat tatcttgttc tcctaaaaaa
      301 tttgcgtgag aaaaactccc ttttatttgt catgcacgtt tttttcgtgg gtgcggccct
      361 actgacatta ttgctggttt tgctgtttag cggattacgt ttgctagtct aaagctgttt
      421 tatttttgcg cctctttgcg cacccatgag tctgattctg attttaaaaa acgttcaaat
      481 cgtggtgcat ctacagaacg catgaaacgg gccgccgaaa tccgaacctg tgcattaggg
      541 tgtttcagca aacgggcagc cagtcgtttt actctctgag gctgagtttt gtaaaaacca
      601 tttttcaaag cttcaaaacc acggcgggcc agaggtgcaa atgatttttc tgcatatagc
      661 tccaaaaatg aagcggtgtt ttctgtggga aacagtgaca aagcatcgag tgccctaaaa
      721 cgaagatagt tgatcaacga cgtatctccg ccaatttcaa tcagcacttt gtctgtatca
      781 tctcccagaa gctgaaatga ctcaacgtcc agcttccagt gacgtccttc cagcagttcc
      841 ataacctgag ccttgtccgg agcagcaact gtgattccgg tcaggttcca gaataaaata
      901 caaatgataa acagatattt catgatcctc ctgttttttc agagaagtgt tgttgaaagc
      961 tgataaattt actttgcaat tggtgaatac ttcaaaacgt gttgctgatg actggacaaa
     1021 gtttcctgtt tttttcctgc agatcgtgat gaagtggtcc aggcagtcca gaacatcaaa
     1081 ttttctccac catatccttc acattcttcg gcagacattt tcccgtttct gtcaaaatcc
     1141 ttggtgctgt ttaagcattc cgcggtatca gtcaaaatat caaatacagt tccgccagac
     1201 tcagtagtat gaaacagacc aagctggtgt cccatttcgt gagccgctgt ttcacccagc
     1261 agttgagcaa ccagtgtgct tccggttgca tgagcagtga gactgatcag cactccgttc
     1321 caggaatttg caattcccat tgaacccgga attcctgctg cattgcccaa gtgttcggag
     1381 tctgaataat cctcaataaa aaacaaatta acaccctctg tcactccctg tgaaacaagt
     1441 gcggaagtag tagaattggt gaatgttccg gaaacttcag catactgagt gtcactgatt
     1501 gtaattgtat cgttgatggt aagtgtgatt ccatttgcag agtagatact tgacattacg
     1561 ctcaaggcag cgctcagatc tccggcagac ctggttgtac cagtgatgta gggctgcaca
     1621 actattgttg ttgaagcaga aacagtaccg gagcgtagtg ttaattttac acggtcgtta
     1681 tttttggctt taaatgtcca tgtgcctgct tttgcagtga agtcgggtga ctgcggaacg
     1741 agaacattgg cataaccaga gcttcccagt ctaccgctgg cctcattgta taaattagga
     1801 gttgaagaag cacttaaaat atctgttcca tcaggatcag ttaatgaata aaattttatg
     1861 ttgtaattgc tgccaaggaa aattgacaaa agaaaagata cgccgtcctc aggcacaaca
     1921 aaaaaaccag caagaagtcc gtcacttcca actgtttgag ttatttcaga gatagcggtg
     1981 ttaagtccat ctatatttgt ttttgcacta ctgacaccgg aaagttcctc a
//
LOCUS       DTVD01000139            8185 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2458_length_8185_cov_390.087085, whole genome
            shotgun sequence.
ACCESSION   DTVD01000139 DTVD01000000
VERSION     DTVD01000139.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_8185_cov_390.087085"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="EYO68_04875"
     CDS             <1..252
                     /locus_tag="EYO68_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC07174.1"
                     /translation="MTRILLEEVFQTDIDQAQDQIIFCGDSPNDAPMFGFFENSVGVA
                     NVLDYEDELEHQPTWLTTKPASSGFVELAAAILDAHSNA"
     gene            655..2589
                     /locus_tag="EYO68_04880"
     CDS             655..2589
                     /locus_tag="EYO68_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HIC07175.1"
                     /translation="MKQQQFLNLASAEEAEERFWQAVQPGPLGEELIPIEHARERILS
                     QNVIAKHNVPYFDRSNFDGFAVRAEDTFGAQETAPVSLKLNPEVLACGVVPEKSVTQG
                     TATTIATGGVMPRGADAVVMIENTLPIEADKSGEAGIKILKAVVPSGGVSLAGSDIGA
                     GEVVLRIGDLLGYRETGTLAALGEAKVWVWRRPKVGIISTGDELVAPGGQMELGKVFD
                     SNATVLGHAVEELGCEPVHFGIVPDEESRLETVLRKALELDFVLISGGTSKGEGDLNY
                     RVFEKYNNPGILVHGVALKPGKPLCLAILAGTPAAILPGFPTSATFTFSKFIAPVLRA
                     MAGRLPEPTTHVKANVPVRLNSDKGRTEFNLVHLVRNDSGFSAYSTGKGSGSITGFAR
                     ADGFMEIPRNTEMVEVDEEVRIQLLGKSAHPPDLMIIGSHCVGLDYLIGEMQKRGVSC
                     KFLAVGSMGGVLAAERGECDLAGTHLLDENAGEYNRHLLTPELHLQKGYRRSQGLLFR
                     KDDSNFTDFKSDFENAIQQIINNAEVRMINRNRGSGTRILLDRLLADQRPAGFFQEAK
                     SHNSVAAAISQNRADWGIAIRSVAEDLGLGFYPIQDEEYDFILPKNRLERPEVALFLS
                     LLQETEIQNKLAKFGLRTTN"
     gene            2656..3198
                     /gene="hemJ"
                     /locus_tag="EYO68_04885"
     CDS             2656..3198
                     /gene="hemJ"
                     /locus_tag="EYO68_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIC07176.1"
                     /translation="MYFLYIKALHIIGVIVWFAGLFYLGRLFVYHQEANLRSEPLRTV
                     MKLQFSQMERRLYYGITWPGLCISIVFGTALIFEIGLQNWLHSKLALAAILVGYHLWC
                     GHLRKQLLNDRCNWSAKQFRFFNEIPTLLLVSIVFIVVFKNEFSWSIFSIILGGLILL
                     ISGTLQWLVKRRKNEAAENN"
     gene            3176..3904
                     /locus_tag="EYO68_04890"
     CDS             3176..3904
                     /locus_tag="EYO68_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07177.1"
                     /translation="MRLPKIIEPVEIMKKYKMQLKHLVLIIFAVLVTGCSWFSDSTEP
                     VNESYEAGKKALEEGNYEIAKSYFREISPDSPFYPQAIWMIQRVPFKKGVVAFEQKQY
                     QIAIFELSKVPLHSPDYAESRRYLKLVNLALLNKQFLNASGQDRFVLVQEIIDIAYEL
                     ADSKLIFESVDLIYTGLDQSTSSRHTRDLIILLGSVVSTNKDLALQQKALNYLLTDFE
                     QLYKHSEVRPEVFRIIGNLKLEMM"
     gene            complement(4032..4763)
                     /locus_tag="EYO68_04895"
     CDS             complement(4032..4763)
                     /locus_tag="EYO68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIC07178.1"
                     /translation="MLPIIGIVLVPGILWGWVFYHAQRYKKVYLPLLLVLFLGGMACG
                     MLALVLNHAVEKYTLFWPEAPLPQIIVLGKSIPLLSSGFWFLVGINEEFAKLLVLLAV
                     VFPSRHLEDPFDGILYAAVVSVGFATMENFYYLDQFGVAVVATRTVITIPAHAFMSVP
                     MGYYAAKSRIALDSSKEIKFRYFLPMATIFQGWLISAFLHGLYDFFLSLKMEREAYLL
                     IIIMGCISVWLGKIALRVSRRKVER"
     gene            4894..6315
                     /locus_tag="EYO68_04900"
     CDS             4894..6315
                     /locus_tag="EYO68_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIC07179.1"
                     /translation="MQRKSLSSPKNEKELNLVTEIIKSGRYFNEISLSYLKEMLRQGE
                     LVSLNADEYLIREDHPKPPELIVLLEGSLAVTSQSLFIMRLDLPGDVVGEMSIINNDP
                     NPFADVIAEENSKVVIFPNHLFKVKGNDTKVSVAYLMFSHILAEKLKQTTAQFLLNKN
                     VRTQEGTLPIIGILDPDKEYRTSIKTILANEWDKARVVEFGSYKKFIENQQKNNFDFL
                     IIDPENFTGGFSRKDSIQILIEICCSHFSPILVVSKYCNKEENRRFLAGLSVTDFLSK
                     PFSDFDLKHIIAKIRKDHYLHKELEQVEITADTDRLTGLANRRRMDEFLEAMVTLYPG
                     EQQPFSLIIADVDHFKHYNDTHGHQMGDVVLASVATIFKNRVRRGDLAARFGGDEFVV
                     ILPKCGKMNALLIGEKLHTAIADEKFPNQEQQPLGNLTCTFGIATFPEDADARELLLK
                     KADDCLYEGKERGRNTVVAAEPD"
     assembly_gap    6383..6482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6503..6946
                     /gene="rplM"
                     /locus_tag="EYO68_04905"
     CDS             6503..6946
                     /gene="rplM"
                     /locus_tag="EYO68_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIC07180.1"
                     /translation="MNTKFARKEDFYNGTHPRAWWIVDAEGRTLGRLATQIAHVLRGK
                     HKTLFSPHVDTGDFVIVVNAEKIHVSGKKNEDKMYYHHTGYPGGIKSARYYELQEKHP
                     ERIVEGAVKGMMPKNALNRRTLQRLKVYTGSEHPHESQQPQPLNI"
     gene            6950..7351
                     /gene="rpsI"
                     /locus_tag="EYO68_04910"
     CDS             6950..7351
                     /gene="rpsI"
                     /locus_tag="EYO68_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIC07181.1"
                     /translation="MVKKATVQYYGTGRRKDSVARVYLRPGKGEIVVNKRPVEDYFGR
                     ETLKMVLRQPLELTESLEKFDIFINVHGGGLSGQAGAIRHGISRALLLALEDSRDVLK
                     KAGMLTRDSRKVERKKYGQPGARKKYQFSKR"
     gene            7463..>8185
                     /locus_tag="EYO68_04915"
     CDS             7463..>8185
                     /locus_tag="EYO68_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07182.1"
                     /translation="MVTILFIFWTLSSVFLTLNVFHPLAKRRSSSFFTLLISFALGWL
                     VGDLLPQWILLNSGIALLFSFSDIFSQTLGWAGLVIHLCCWIILIIRLWIILNLPARI
                     DQQLEEQLGSIWQNSSTFFSPPDNFLEVNWHSWLNPNSILEDPRIEIIRNHVFFEEDD
                     LRLRLDIYRPRSSKKKRPVLLQIHGGAWISGSKRQAAFLMTHMAAQGWVCFSVGYRFS
                     PDIKFPQHLIDIKRALKWIRNHA"
BASE COUNT         2392 a         1666 c         1747 g         2280 t
ORIGIN      
        1 atgacaagaa ttttactgga ggaagtattt cagacagaca tcgatcaggc acaggatcaa
       61 atcatctttt gcggagactc cccaaatgat gcgcctatgt tcggcttttt tgaaaattcg
      121 gttggcgttg ccaacgtttt ggattacgaa gatgagttgg aacatcaacc aacctggctg
      181 actacaaaac ctgcctcctc tggatttgtg gaactcgctg cagcaatact ggacgctcat
      241 tcgaatgcct gaaagtacat cgcagcaaaa acccgcgcgt ccaatcttcg ccattgtcat
      301 tccgggcgga gcacagcgca gacccggaat ccaggggaac tgcttcaaag ctgatttccg
      361 aactgaaatc actctttaac ctcgctcaag tcaggattga ctgccttgat tttttccttt
      421 tgtcatcccg gacttgatcc gggatccagc taaattacgc ccccgctttc taaacacaat
      481 attgacaaac taagtacatc tgttgtatcc ttttttcatg aacagcagtg aaatcaagcg
      541 atgtttttcg aaacagtgaa caaactttgt ttccgaaaaa ccgcgccgct cttcctttaa
      601 ctttacctga gtttataccg gccatcaaat ccgtgagtta aatgtgtttt caaaatgaaa
      661 cagcagcaat ttttaaattt agcttcagca gaggaagcag aggagcgttt ttggcaggca
      721 gttcaacctg gaccacttgg tgaagaactg attcccatcg aacacgcacg cgaaagaatt
      781 ttgtcgcaaa atgtgattgc caaacacaac gttccctatt ttgatagaag taatttcgac
      841 ggctttgcgg tacgtgcgga agacactttt ggcgcacagg aaactgctcc ggtttccctg
      901 aaattgaatc cggaagtcct ggcctgcgga gttgttccgg aaaaatcagt cactcagggt
      961 actgcgacaa ccattgctac tggcggagtt atgccgcgcg gtgcagacgc tgtggtgatg
     1021 attgaaaaca ctttgccgat tgaagctgat aaatccggag aggcaggaat taaaatcctc
     1081 aaagctgttg ttccgtcagg tggagtgtca ctggcaggct cagatattgg ggcaggggag
     1141 gtagttttgc gaatcggcga cctgctgggt tatcgggaaa ccggtacact ggcagcactg
     1201 ggagaagcaa aggtctgggt ctggcgtcgt ccaaaagttg gaatcatttc gactggagat
     1261 gagttggttg cacccggagg acagatggaa ctgggaaagg ttttcgattc aaatgcaacc
     1321 gttctgggcc atgctgttga agaactgggt tgcgaacctg tgcattttgg aattgttcca
     1381 gatgaagaat cacgccttga gacagttctc cggaaagcac ttgaactgga ttttgtactg
     1441 atatccggag gcacatcaaa aggggaggga gacctgaatt atcgggtttt tgaaaaatac
     1501 aataatcccg gaattttggt acacggtgta gcactaaaac cgggaaaacc tctttgtctt
     1561 gcaattcttg ctggaactcc agcggccatt cttcccggat ttcccacatc tgccactttc
     1621 actttcagca aatttattgc tccagtcctg cgggccatgg ccggacggct tccggaaccc
     1681 actactcacg ttaaagcaaa tgttccggtt cgtttgaatt ctgataaagg acgtacagaa
     1741 tttaatcttg tccacctggt tagaaatgat tccggtttca gcgcatattc gacaggaaag
     1801 ggttccggtt caatcaccgg atttgcgcgt gcagacggct tcatggaaat tccccgcaat
     1861 accgaaatgg ttgaagttga tgaagaagtt agaatccaat tacttgggaa atcagcacat
     1921 cctccggatt tgatgatcat cggaagtcac tgtgtcgggc tggattattt aatcggagaa
     1981 atgcaaaaac gcggagtttc atgtaaattt ctggcagttg gaagtatggg aggagtttta
     2041 gctgctgaac ggggtgaatg cgatttggcc ggtacccatt tgctggatga aaatgcagga
     2101 gaatacaacc gccatttact tactcctgaa ctgcatctgc agaagggata ccgacgcagc
     2161 cagggcttgc tgtttcgtaa agacgattcc aattttacag actttaaatc agattttgaa
     2221 aatgcaattc agcaaattat caataatgct gaagtgcgca tgattaatcg aaaccgggga
     2281 agcggcacac ggattttact agaccgtttg ttagcagatc aacgtcccgc aggttttttt
     2341 caggaagcaa aatcgcacaa ttctgtggct gcggcaatat cccaaaatcg tgctgattgg
     2401 gggatagcaa ttcgttctgt tgccgaagat ttgggacttg ggttttatcc aattcaggat
     2461 gaagaatatg attttatttt accgaaaaac cgcctggaac gtcctgaagt cgctcttttt
     2521 ttaagtttgt tgcaagaaac agaaatccaa aataaacttg caaaatttgg cctgagaact
     2581 acaaattaat ttaaagtttt tcagggatat accgtcaccc tcactctcgt taatataact
     2641 caattaattt gaatcatgta ttttctttac attaaagcac ttcacatcat tggcgttatt
     2701 gtgtggtttg caggactctt ttacctggga cgactttttg tatatcatca ggaagcaaat
     2761 ctccgttctg aaccgttacg gacagtaatg aaattgcagt tttcgcagat ggaaagacgt
     2821 ctttattacg gaattacttg gcccggcctg tgcatctcga ttgtctttgg aactgcctta
     2881 atttttgaaa ttggtttaca aaattggctg cactctaaat tagcactcgc ggcaatattg
     2941 gtcggatatc atttgtggtg cggacatctt cgcaaacaac tgctgaatga taggtgcaac
     3001 tggagcgcaa aacaatttcg tttttttaat gaaatcccaa cgctgctcct ggtcagtatc
     3061 gtttttattg tggtatttaa gaatgagttt tcgtggagca ttttttcaat tatcctgggt
     3121 ggtttaattt tgctaattag cggaacgcta cagtggctgg taaagcgtcg gaaaaatgag
     3181 gctgccgaaa ataattgaac ctgtcgagat aatgaaaaaa tacaaaatgc agcttaaaca
     3241 tctggttttg attatatttg ctgttctggt aacaggctgc agttggttta gcgattctac
     3301 agaaccagtg aacgaatctt atgaggccgg taaaaaagct cttgaagaag gtaattacga
     3361 aatagccaag tcttactttc gtgaaatcag tccagattct ccattctatc cgcaggcaat
     3421 ctggatgatt cagagagtgc ctttcaaaaa aggagtagta gcttttgaac agaaacagta
     3481 ccaaattgca atttttgaat tgtccaaggt tccacttcac tctccagatt atgcagaatc
     3541 ccggcgatat ctgaagctgg tgaatcttgc cctgctgaac aaacagtttc tgaatgcttc
     3601 cggtcaagat cgttttgtgc tggtccagga aatcatagat attgcatatg aattagcaga
     3661 ctcaaaacta atttttgaga gcgtggatct tatttatacc gggcttgatc agtctacttc
     3721 aagcaggcat accagggatt taatcattct gcttggaagc gttgtttcta ccaataaaga
     3781 tctggcatta cagcaaaaag cccttaatta tttgttgact gattttgagc agttgtacaa
     3841 acattcagaa gttagacctg aagttttcag aattattgga aacctcaaat tggaaatgat
     3901 gtgataaccg gtttatattg cagttgcagt gtccttcaaa aactattcaa aagttaaatt
     3961 tataccaggc aggcaattat tgtttagatt ttaaattcaa actaaaacca ataacataat
     4021 cctctcttcc ttcatctttc aaccttcctc ctgctcaccc tcagagcaat ttttccaagc
     4081 cacacactga tgcatcccat tatgatgatc agtaaatatg cttctctctc catcttcaaa
     4141 gacagaaaaa aatcatacag gccgtgcaga aatgcagaaa tgagccatcc ttgaaatatt
     4201 gtggccatcg gcaggaaata tctgaatttt atttctttgg aggaatcaag cgctatgcgt
     4261 gattttgccg cataatatcc cattggtaca ctcataaaag catgtgcagg aatggtaatg
     4321 accgttcttg tagcaacaac tgccactcca aactgatcca gataataaaa gttttccatt
     4381 gttgcaaacc caactgaaac tactgcagca tagagaattc catcaaaagg gtcctccaga
     4441 tggcggcttg gaaaaactac tgccagcaat acaagcaact ttgcaaactc ctcatttata
     4501 ccaactagaa accagaagcc tgaactaagc aggggtatcg acttaccaag tacaataatc
     4561 tgtggtaatg gtgcttccgg ccaaaatagt gtatattttt caaccgcatg atttaagact
     4621 agagccagca tcccacatgc cattcctcct agaaaaagga cgagcagtaa gggcaggtaa
     4681 acttttttgt agcgctgtgc atgataaaat acccagcccc agagaatccc tggaaccagg
     4741 acaattccga taatgggcag catactaact ttttctaagt gttgtaattg ctttttgttc
     4801 gtggcatagt ttccgtagaa actgtaaaaa atatcttaca tgttgactgg agggagcctg
     4861 cagcatgtag caagaatgta aagaaaaact ccaatgcagc gaaaatcact ctcatcaccc
     4921 aagaacgaga aagaactgaa tcttgttaca gaaatcatca agtccggacg ctatttcaat
     4981 gagatcagcc tatcttacct taaagaaatg ctgcgtcaag gtgaattagt ttccttgaat
     5041 gctgacgagt acctgattcg agaagatcat cccaaaccac ccgagttgat agtccttctg
     5101 gaaggttctc tggcggtaac ttcacaaagt cttttcatca tgcgtcttga tcttcccgga
     5161 gatgttgtcg gagaaatgtc aatcatcaac aacgatccta atccattcgc cgatgttatt
     5221 gcagaagaaa attctaaagt ggtgattttc ccaaatcatc tcttcaaagt taaagggaat
     5281 gacacaaaag tctctgttgc atatttgatg ttttcacata ttcttgcaga aaaactgaaa
     5341 caaacaaccg cacagttctt gttgaataaa aatgtccgga cacaagaagg aacactcccg
     5401 ataatcggca ttcttgatcc ggacaaagaa tatcgtactt caatcaaaac catacttgca
     5461 aatgaatggg acaaggcccg agtagttgaa tttggatcat acaaaaaatt tattgaaaat
     5521 cagcaaaaga ataatttcga ttttctaata attgatcctg aaaattttac aggcgggttt
     5581 tctcgaaaag attctattca aatcttgatt gaaatatgct gctctcattt ttcgcccatc
     5641 ctggttgtta gtaaatattg taataaagaa gaaaaccggc gctttcttgc gggactcagc
     5701 gtgacagatt ttttaagtaa accattttca gattttgact tgaagcacat aattgccaaa
     5761 atccgaaaag atcattatct tcataaagag ttggaacagg tagagattac tgctgacaca
     5821 gacagactta ccggattagc caaccgccgc aggatggatg agtttctgga ggcaatggta
     5881 accctttacc cgggagaaca acaaccattc tcgcttataa tcgctgatgt ggatcacttt
     5941 aaacattata atgatacaca tggtcatcaa atgggcgacg ttgtccttgc ctcagttgcc
     6001 acaatcttca aaaacagggt ccggagaggt gacttggctg cacgttttgg aggtgatgaa
     6061 tttgtagtca ttttaccaaa atgtggaaaa atgaatgcac ttttgattgg tgaaaaactg
     6121 cacacggcaa ttgctgatga aaaatttcca aatcaggaac agcaaccgct gggtaatctc
     6181 acttgtacat ttgggattgc aacatttccg gaagatgcag atgcaaggga actactcttg
     6241 aaaaaggctg atgactgttt atatgaggga aaagagagag gaaggaacac agtggttgcg
     6301 gccgagcctg actgagattt gattccacaa tgataaggcc ctcatccggg aataaatcaa
     6361 tggtgcagta cataaatcgt gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnaaaaaaga ttgagataat taatgaatac taaatttgcc agaaaagagg atttctacaa
     6541 tggcacccac ccgcgagcct ggtggattgt ggatgcagag ggccggacac tgggccggtt
     6601 agcaacacag attgcccatg tgctgcgtgg aaagcataaa actttgtttt ctccacatgt
     6661 agacacagga gattttgtaa ttgtggttaa tgcagaaaaa atccacgtaa gcggtaaaaa
     6721 aaatgaggat aaaatgtact atcatcatac tggatatcct ggaggaatta agagcgccag
     6781 atattatgaa ctgcaggaaa aacatccaga gcgcattgtg gaaggtgctg tcaaaggtat
     6841 gatgccaaaa aatgcactca accgacgtac ccttcagcgt ttaaaagttt ataccggttc
     6901 ggagcaccca cacgaatctc aacaacctca gccccttaat atctgaccta tggtaaaaaa
     6961 agcaactgta caatactacg gaactggacg tcgaaaagac tccgtagccc gcgtttacct
     7021 caggccggga aaaggtgaaa ttgtagtaaa caaacgacct gttgaagatt attttggtcg
     7081 agaaactctt aaaatggttc tccgtcagcc cttggaacta accgagtcac ttgaaaaatt
     7141 tgatatattt atcaacgttc acggcggcgg actttcaggt caagcgggtg ctattcgaca
     7201 tggaatttca cgtgcattat tacttgctct cgaagactca cgtgatgttc tgaaaaaggc
     7261 cggaatgctg acccgtgact cccgcaaagt tgaacgtaag aaatacggac agccgggtgc
     7321 acgtaaaaaa tatcaatttt ctaaacgtta atttattggc tgtgtttgac tgcaatgtaa
     7381 gtgttaaaca cagtttctaa ctttccttac ttttctctta gtaattctta aagaggactc
     7441 tgcattgctt ttataaagcc gcatggtcac aattctcttc atattctgga cattaagttc
     7501 tgtatttctg acactcaatg ttttccatcc tcttgctaaa cgcagaagtt cttctttttt
     7561 cacattactt atttcatttg cactgggatg gttagttggt gatttgcttc cgcagtggat
     7621 actgcttaat tcaggaatag cccttctttt ctcattttcg gacattttca gccagacgct
     7681 cggttgggct ggtcttgtga ttcatctttg ctgctggatt atcctgatta tccgcctctg
     7741 gataatttta aatttacctg cacgcattga tcaacaatta gaagaacagt tgggcagtat
     7801 ttggcaaaac agttcaacat ttttctctcc tccagacaat tttctggaag ttaactggca
     7861 cagttggtta aatccgaaca gtatcttaga agatccacgg atagaaataa taaggaacca
     7921 tgtgtttttt gaagaagacg atttgcggct caggctggat atttatcgtc ctcgaagttc
     7981 aaaaaagaag cgtcccgtat tattgcaaat tcatggcgga gcctggatca gcggcagcaa
     8041 acggcaagcc gcatttttaa tgacacatat ggctgcacaa ggctgggttt gtttttcagt
     8101 cggctaccgc ttcagtccgg acataaaatt ccctcagcat ttgattgaca tcaagcgtgc
     8161 attgaagtgg attcgtaacc atgca
//
LOCUS       DTVD01000140            8165 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2464_length_8165_cov_532.073490, whole genome
            shotgun sequence.
ACCESSION   DTVD01000140 DTVD01000000
VERSION     DTVD01000140.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_8165_cov_532.073490"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2942)
                     /locus_tag="EYO68_04920"
     CDS             complement(<1..2942)
                     /locus_tag="EYO68_04920"
                     /inference="COORDINATES: protein motif:HMM:PF00400.30"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIC07183.1"
                     /translation="MINCQNEMQYGVLMKNLFVFIRKIIPEKSCCFVFIILLLCLSSS
                     TAAKEEDSEKLKKDYFLSQSLKLGKKPVQSAVFSPNDQQAVILSGSSSLEIFRIQNGK
                     RLRVISSHEHKAISLVLHAGGKLAVTGGQDDTVRIWSTDQSTAQAVMRGHLSAVSVLA
                     LNTGGEILASGSLDGTVILWNMKEHELLKSTKVIGKSSIKSLAFHPNGRILAIGGEDG
                     SLQFRSIPEMKLITTLSGHKNSINDLEFNPRGDLFVSGSEDGKVIIWDWKVKKQRSKI
                     NFEDAVSTISINPKLAEIVVGTAGGRLETWSLAKGTLLHQITESGPLISSAGYDRNGS
                     RIISALEDGTVEIWENGVSLHLKTFSGHERIVESLDFSSNNKFLISSSSDKSVRIWDL
                     KTNENVHNFDIGNHRVQDLRFAPGSQQFATAGTDSSVIIWDAKDGSRVRTLKFHKGKI
                     NALSYHPEDSVLLSAGSDRKWVLWDLVSGKSLLSHKGHTSQILAASFSPDGKRFATAG
                     SDLFIKIWEYPQGEELGKLKGHKKPVTTLAFSPDGKLLASGSQDHQIFLWRLEPEISK
                     TPFRKLEGHSYIVTQVFFSIAGKALISISKDKTMRLWEVKSGKMLRILHGDSTPLVAA
                     ALSPDGKLIALSNLTNDITILTFPTDIPELQDDTGDVSASGETDNSASLQTSESDSDA
                     SDKYGIDLENLKEMEKLPMTAEELRAYAVTETDDLSTVQYEMRQRLNLLLKTKDTCLH
                     AAEMEKLALKILVIVPDDLAAYHALVKTSILKQDFATLSLFVSSGYFAELDQELYDYI
                     SILEVRNIFDNLRFELFDQSFVRQGNKQKIKLANCKGNPLSVSLSGISRNLHYPVEFL
                     KNINSIPRLIDLREYMNLPQKEFQDRMFAEIERILGSRTPHPTSYLPKTPEERSEKVP
                     VGILNLNLEKAQTWKNDGMASFSLRKVGGQWQSYQTDRDNRIVMHLPAGSYSLKVAGV
                     LRKTFFLIA"
     gene            3105..3929
                     /gene="tatC"
                     /locus_tag="EYO68_04925"
     CDS             3105..3929
                     /gene="tatC"
                     /locus_tag="EYO68_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIC07184.1"
                     /translation="MSKTKSEQKKDPDQNENSDDVPMPLTSHLGELRKRLVRIIIVIL
                     VVFVGATVVEMELDQPILSAFRAPLDARNVPLVFLELTEPFFTYLRIGLYAAIFLSFP
                     YLLGQIWLFVRPALFSKERNAVWPFILLSYPLFVGGGLFGYFVVIPLGYDFFLGFENK
                     FTLPSLSMASYLSLTIHLLFAFGLVFELPTVSFILTRLGLINAAWLRKNRKYSLVVIF
                     IGAAILTPPDVFTQTLMAGPLIILYEISILVSQMAGKKEKVPAPEEQKTQDKKSKS"
     gene            complement(4114..4728)
                     /locus_tag="EYO68_04930"
     CDS             complement(4114..4728)
                     /locus_tag="EYO68_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC07185.1"
                     /translation="MPDNYTLLQRKYWIFDLDGTLTVAVHDFNAIRNELGIPAGQPIL
                     KTIESLPENESLALQQKLQEIEENLARNARPAKGVKNLLEALHRRNYHLGILTLNSRE
                     NAWLTLEALDLADYFNEYSVIGRWCVEPKPSPKGIHRLLNHWEVAANDALIVGDYLYD
                     LQVGRAAEIATVHVDPKGEFSWPELSDIQANSLDELADLLAENS"
     gene            4769..5554
                     /locus_tag="EYO68_04935"
     CDS             4769..5554
                     /locus_tag="EYO68_04935"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC07186.1"
                     /translation="MHSTDISSVRTSQKSDAGLSCKNLSRQISGSWIWSKINLHLAPG
                     DKLALTGSSGSGKSLLLRTLAGLDVIESAPSGETGTINFDGISLHDWEMPQYRTKVSF
                     IPQNPVFFDETVETNLKRAFQLKAHQHRRYDQKQILAWLEQFSLPIAAGNSSGVGDYS
                     EFLQRPAKDLSGGEAQITALLRVLQLEPQVLLLDEPTASLDAELTGRFENLLEKWQKE
                     LPDSNSDTANTTPQRAWIWVSHNPDQLRRMCRNMFSLESENGN"
     gene            5544..6344
                     /gene="fetB"
                     /locus_tag="EYO68_04940"
     CDS             5544..6344
                     /gene="fetB"
                     /locus_tag="EYO68_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HIC07187.1"
                     /translation="METDYIFLTIGQLLTASVLMAVNIGLSLILKLGLAKQWSIASLR
                     MVGQLLLVGFLLDWVFALNNPFWILGVALFMATIASITAVGRTRKRFSAIYLNSFISI
                     LGAGFFVMFFALSGVLQIDPWYSPQYLFPLLGMVLGNTLNGISLALERFMDSLSVRRK
                     EVEMLLSLGATSWEAAHDLIRDALRTSMIPTLNSMLVMGIVSLPGMMTGQILAGVSPG
                     EAVRYQIMMIFVIASGAALGATLVVILAFRALFNSRHQLLLKHLRTVS"
     gene            complement(6373..7296)
                     /locus_tag="EYO68_04945"
     CDS             complement(6373..7296)
                     /locus_tag="EYO68_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HIC07188.1"
                     /translation="MKTIVIVNPQAGNGRTEKIWPNIESALEKSIGSFEVLQTTCRGD
                     ATDLSRRILAVDTARIVAVGGDGHLNEVLNGFIENDLPVNPEARLSFVMTGTGCDFQR
                     SLGISGKWQNAAAKLKDAKVRKIDVGKVTYTAADKTQKIRYFDNIASFGLSGAVDHCL
                     EHSRLRDYLGGSPLFLWATIKTVFTHPNQSIRFRIDDGPEEEICSRLGLLANGRYFGG
                     AMHAAPEAELDDGLLDLLMLKEISVAKFLWHLPKIYKGTHLKIPEIFFQKVRKFTAES
                     SEQVILDIDGESPGYLAATFEVLPKILNLQI"
     gene            7402..7605
                     /locus_tag="EYO68_04950"
     CDS             7402..7605
                     /locus_tag="EYO68_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07189.1"
                     /translation="MKINLTMSLLFGAVLMMLTACNWNKTEKAQQQIYAPGPDATSAL
                     SGTLQRNQDYYKESDGDLLKKMR"
     gene            complement(7614..8030)
                     /locus_tag="EYO68_04955"
     CDS             complement(7614..8030)
                     /locus_tag="EYO68_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIC07190.1"
                     /translation="MLWGINGYSVGRPHNLGLKNNMLAPCPSSPNCVLSQASDAKHKI
                     KPIYYATSVEEAKEKLIKVIQSMDGTRIIKQDEVYWHVEFTTRWLRFIDDVEFYFPES
                     EALIHLRSASRSGYWDLGVNRKRVEEIRSRFEELAR"
BASE COUNT         2238 a         1822 c         1647 g         2458 t
ORIGIN      
        1 gcaatgagga aaaatgtttt tcttaaaact cctgcaactt tcagagaata acttcctgca
       61 ggaagatgca tgacaattct gttatcccga tcggtttggt agctttgcca ctgaccccct
      121 actttacgta aactaaatga agccatgccg tcatttttcc aggtctgggc tttttcaaga
      181 tttaaattca gtattcctac cggaaccttt tctgaccgct cttccggagt tttcgggagg
      241 tatgaagtag gatgaggagt cctgcttccg agaatccgtt ctatctcagc aaacatcctg
      301 tcctgaaact ctttctgcgg caaattcata tattcccgca agtcaatcaa acgaggtata
      361 gaatttatat tttttaaaaa ctcaactgga taatgtaagt tccgggaaat tcctgataag
      421 ctgactgaga gaggattgcc tttgcagttt gccaatttta ttttttgttt gttaccctga
      481 cgtacaaacg attgatcaaa aagctcaaat cgtaaattat caaaaatatt tctaacttcc
      541 aatatggaaa tataatcata aagttcctga tccaattccg caaaataccc tgaacttaca
      601 aacagactca gtgttgcaaa atcctgtttg aggatagacg tcttgaccaa tgcatgatag
      661 gcagccaaat catcaggaac aatgactaat attttcagtg ccagtttttc catttctgca
      721 gcatgcagac acgtgtcttt tgttttaaga agcagattaa gacgctgccg catttcgtat
      781 tgaactgttg ataaatcgtc ggtttccgtc acagcatagg cacgtaattc ttctgcagtc
      841 atcggcagtt tttccatttc cttcaaattc tccaagtcga ttccgtattt atccgatgca
      901 tccgaatctg actcagaagt ttgtaaactt gctgaattat cagtttctcc ggatgctgaa
      961 acatcacctg tatcatcctg caactccgga atatcggttg gaaatgtgag gatggtaata
     1021 tcatttgtca ggttgcttaa tgcaattaat ttcccgtctg gacttagcgc agcggcaaca
     1081 agaggagtac tgtcaccatg aagtatgcgc agcattttcc cgctttttac ttcccacagg
     1141 cgcatcgttt tgtctttgga aattgaaatc aatgcttttc cagctatgga aaaaaagacc
     1201 tgagtaacaa tgtaactgtg cccttccagt tttcggaaag gagtctttga aatttccggt
     1261 tcaagtctcc atagaaaaat ttgatggtcc tgtgaaccgc ttgccagcaa tttaccatcg
     1321 ggactgaagg ccagagttgt aacaggtttc ttgtgtcctt tgagttttcc gagctcttct
     1381 ccctgagggt attcccagat ttttatgaaa aggtcactgc cggcagtggc aaagcgcttg
     1441 ccgtctggag aaaaagatgc cgctaatatt tgagaggtat gccctttatg agaaagcagg
     1501 gactttccgg aaaccaaatc ccatagtacc cacttcctgt cagaaccagc agaaaggagt
     1561 actgaatctt caggatgata gctgagagca ttgatttttc ctttgtgaaa tttcaaggta
     1621 cgaacgcggc ttccatcttt tgcatcccag attatcaccg aactgtccgt tcccgcggta
     1681 gcaaattgtt ggcttccggg agcaaaacga agatcctgca ccctgtggtt gccgatatca
     1741 aaattgtgta cgttctcatt tgttttcagg tcccagatac gtacgctttt atcggaggag
     1801 gaactgatga gaaatttatt gtttgatgaa aaatccagcg actctacgat cctttcgtga
     1861 ccactgaatg ttttcagatg cagtgaaacc ccattttccc atatctccac agttccatct
     1921 tccagtgcgg aaataatcct gcttccgttt ctgtcatatc ctgcactgct aatcaagggt
     1981 ccggattcgg tgatttgatg cagcaatgtt ccttttgcaa gactccatgt ttcaagtctt
     2041 cctcctgcag ttccaacaac tatttctgca agttttggat tgatgcttat tgttgaaaca
     2101 gcatcttcaa agtttatttt ggaacgttgt tttttgactt tccagtccca gataatcact
     2161 ttcccatcct cagatccgga aacaaataaa tcgccccgtg gattgaattc aagatcatta
     2221 atcgagtttt tatgaccaga gagggtggtt atcagtttca tttcagggat acttcgaaat
     2281 tgcaaacttc catcttctcc gccaatggcg agaattctgc cattcggatg gaaggccaga
     2341 ctttttatgc tgcttttacc gataaccttg gtagatttca gcaattcatg ttctttcatg
     2401 ttccacagga taacggttcc gtcaagactt ccggaagcca aaatctctcc tccggtatta
     2461 agagcaagta cggaaacagc gctcagatga ccccgcataa ctgcctgtgc agtgctctgg
     2521 tcagtactcc aaattctaac cgtgtcatcc tgacccccgg tcaccgccaa ctttcctccg
     2581 gcatgaagga caagtgaaat ggccttatgt tcatgactgg aaattacacg cagtcttttt
     2641 ccattttgaa ttctgaatat ttccagagaa gaacttccgg ataagatgac agcctgctgg
     2701 tcatttggtg agaaaacagc ggactgaacc ggtttctttc ccagcttcaa actttgcgag
     2761 aggaaataat cttttttaag tttttcagag tcttcctcct ttgccgccgt agatgaagac
     2821 aaacagagca gcaaaatgat aaaaacaaaa cagcagcttt tttccggtat tattttccgg
     2881 ataaagacaa aaaggttttt catcaacact ccatactgca tctcattttg gcaattaatc
     2941 atttgtttgt cgtgcaaata aaatcagttt atggtacaaa atatggttgg cgttcagtcg
     3001 ttaatcagga gaaataaaaa aatagctgaa aaacttaggc tcgaatgtct gtttgtattc
     3061 aactcaaatt ataattattt accatatttt tgtagccttt aataatgagc aaaaccaaat
     3121 ctgaacaaaa aaaagatccg gatcaaaatg aaaattcaga tgatgtccct atgcctttaa
     3181 cctctcatct tggtgaactg cgcaagcgtc ttgtccgtat aattattgta atcctggttg
     3241 ttttcgttgg tgcaactgtt gtagaaatgg aactggatca accaattctt tcagcctttc
     3301 gcgcaccatt agatgcacgc aacgtccccc tggtctttct agaattaaca gaaccgttct
     3361 tcacatatct gcggatcggg ctatatgctg caattttttt atcattcccc tacctgcttg
     3421 gtcaaatctg gttgtttgtt cggccggcac ttttttcaaa agaacgcaat gcagtctggc
     3481 cctttatttt actttcatat ccactttttg tcggcggagg tcttttcggt tattttgtcg
     3541 tgattcccct tggttatgat ttctttttgg gttttgaaaa taaattcact ttgccatcct
     3601 tgagtatggc cagttatctg tcgctgacaa ttcacctgtt attcgcattc ggactggttt
     3661 ttgaactgcc gacagtctca ttcattctca ccagacttgg actcatcaat gcagcctggc
     3721 tacggaaaaa ccgtaaatat tcgctggtcg tcatttttat tggtgccgca atcctgactc
     3781 ctccggatgt gtttacacaa acattaatgg ccggacctct tattattcta tacgaaatca
     3841 gcattcttgt ctcacagatg gctgggaaaa aagaaaaagt tccggcgccg gaagagcaga
     3901 aaacccagga taaaaaatcc aaatcataaa ctgcacaact gcaacccgga ttaagtccga
     3961 tatgacgaaa gaaatacttt ttccgctggt ctctattatt atctgcagaa ttgaacccaa
     4021 atactaccct caaagtaaag agtaaggcag gagtcatcta acaccaaaga ggagaaagtc
     4081 gcaagtgaca agcgggaaga tttgataata tcgtcagctg ttttcagcta ataaatcggc
     4141 aagttcgtct aaagaatttg cctgaatatc tgacagttca ggccaggaga attctccttt
     4201 cggatcaaca tgaaccgtag caatttccgc ggcacgtccc acctgcaggt cataaagata
     4261 gtctcccacg attaacgcgt cgtttgctgc aacttcccag tgattcagca acctgtgaat
     4321 tcccttcggg gaaggtttcg gttctacaca ccagcgtcca ataacggaat attcgttgaa
     4381 gtaatctgcc aaatcgagag cctccagcgt gagccatgca ttctccctgg agtttaaggt
     4441 gagtatgccc aaatgataat tcctgcgatg gagtgcttcc agtaaatttt ttacaccctt
     4501 tgcaggtctt gcgtttcgcg ccaggttttc ttctatctcc tgaagctttt gctgaagagc
     4561 aagtgattca ttttccggaa gagattcgat agttttaagt atgggctgtc ctgctggaat
     4621 tcctaactca tttcggatag cattgaagtc atgtactgct acagtaagag taccatcgag
     4681 atcgaaaatc cagtatttcc gttgcaaaag tgtataattg tctggcataa agactgtcaa
     4741 tgtaatcgaa tagacaaagg ctaaagtaat gcattctacg gacatctcat ctgtgaggac
     4801 aagtcaaaaa tctgatgccg gactatcctg taaaaacttg agccgtcaaa tttcaggcag
     4861 ctggatttgg agtaagatca acctgcattt agctccggga gataaacttg ctttgacggg
     4921 ctcttccgga agcggtaaga gtctcctgtt acggacatta gccggattag atgttattga
     4981 gtccgcacct tccggtgaaa ccggaactat taattttgac ggtatatccc tgcacgactg
     5041 ggaaatgcct caataccgca ccaaagtcag cttcatccct caaaatcctg tgtttttcga
     5101 cgaaaccgtt gaaacaaatt taaagcgtgc attccagtta aaagcacatc aacatcgccg
     5161 ctacgaccaa aaacaaatcc tggcgtggtt ggaacaattt tcgctgccga tcgctgccgg
     5221 taattcttca ggagttggtg attattctga atttttgcaa cgtcctgcaa aggacctttc
     5281 cggaggagaa gcccagatta cagcactact ccgggtactg caactggaac cgcaggtgct
     5341 tttgcttgat gaacccactg catcactgga cgcagaactg actggtcgtt tcgaaaatct
     5401 gcttgaaaaa tggcaaaaag aacttccgga tagtaattcg gatacagcaa atacaactcc
     5461 tcaacgagcc tggatttggg tcagtcataa tccggatcaa ttacgaagaa tgtgccgcaa
     5521 catgttttcc cttgaatcag aaaatggaaa ctgattatat ttttctcact atcggacagt
     5581 tgctgactgc ctcagtcttg atggcagtca atattggttt gtcgcttatt ttgaaacttg
     5641 ggctggcaaa gcagtggagt attgcttcat tgagaatggt gggacaactg ctgctggtcg
     5701 gctttttatt ggactgggtt tttgcgctta acaacccttt ttggattctt ggagttgcat
     5761 tgttcatggc aacaatcgct tccatcactg cagttggacg tacacgaaaa cgattttccg
     5821 caatttattt gaacagtttc atttcaatcc ttggggccgg attttttgta atgttttttg
     5881 cactttctgg agttctgcaa attgatccat ggtattctcc acagtacctt ttccctctcc
     5941 tcggcatggt tttggggaat acactcaacg gaatttcact ggcactggag cgttttatgg
     6001 acagcctttc tgtgcgacgc aaagaagttg aaatgctgct cagcctgggt gcgacatcgt
     6061 gggaagccgc acatgactta attcgtgatg ctttgcgaac aagcatgatt ccaacgctca
     6121 attcaatgtt ggtgatgggt attgtaagtt tgcccggaat gatgaccggt caaatattgg
     6181 ccggcgtcag tcccggagaa gcagtccgtt atcaaataat gatgattttt gttattgcct
     6241 caggtgcagc tttaggcgcg accttagttg ttatcctggc cttcagggca ctgtttaatt
     6301 ctcgacatca actcctgttg aaacacctgc gaacagtctc atgagtttgt tttagtattt
     6361 tttgctgctg aatcatattt gcagattcaa aatcttcggc aggacttcaa aagttgcagc
     6421 caaatatccc ggagactcac cgtcgatatc gagaataacc tgttcagaac tttcagcagt
     6481 gaatttacga actttttgaa aaaatatttc tggaatcttc aaatgtgtac ctttgtatat
     6541 ttttggcaga tgccacagaa attttgccac agaaatttct ttcagcatta acaaatcgag
     6601 caacccgtcg tccagttctg cttccggagc ggcatgcatt gctccgccaa aataacgtcc
     6661 gttggccaat aagccgagtc ttgagcaaat ttcctcttcc ggaccatcgt caatacgaaa
     6721 acggatactc tggtttgggt gagtgaaaac ggttttgatg gtggcccaca aaaagagcgg
     6781 tgatcctccc aaatagtctc gcagtcttga gtgttcaagg caatgatcta ctgctccgct
     6841 gagaccgaaa cttgcgatgt tgtcaaaata gcggattttc tgtgtcttat ctgctgcagt
     6901 gtaggtgact tttccaacat caattttgcg cacttttgca tctttcagct ttgctgcagc
     6961 attctgccac ttgccggaaa tgcccagtga acgctgaaaa tcacatcctg ttccagtcat
     7021 cacaaaactc aatcttgctt ccggatttac cggaagatcg ttttcaataa atccgttcag
     7081 cacttcatta agatgaccat ctccaccaac cgccactatt ctcgcagtat ctactgctaa
     7141 aattctgcgg cttaaatccg tggcatcacc ccgacaagtt gtctgcaata cctcaaacga
     7201 accaatgctt ttttccagag ccgattcaat gtttggccaa atcttctcag ttctgccgtt
     7261 tcctgcttga ggattaacta tgacaatggt tttcacatgt ctccggaact aattgtgaca
     7321 aatagcttgc gacagtataa agagtttttt aaagtagttg cagataatga cattgagcac
     7381 ataaaaatac aggaaaactg aatgaagatc aatctgacaa tgagtctgct ttttggagca
     7441 gtactaatga tgctgactgc atgcaactgg aacaaaacag aaaaagctca gcagcaaatt
     7501 tatgccccag gaccggatgc gacttctgct ctcagcggaa ctctacagcg taaccaggac
     7561 tattacaaag aatccgatgg agatctgctg aaaaaaatgc gctaagttca gttctacctc
     7621 gcaagttctt caaaccttga acggatttcc tctacccgtt tccggttgac tcccaaatcc
     7681 caatatcctg aacgggatgc cgaacgcaga tgaatcaatg cttcggattc cggaaaataa
     7741 aactcaacat cgtctataaa acgcagccag cgtgtggtga attcgacatg ccaatagact
     7801 tcgtcctgct taataattcg tgtgccatcc atcgactgta ttaccttgat tagtttttct
     7861 tttgcctctt ctacagatgt tgcataataa attggcttaa tcttatgttt tgcatcactc
     7921 gcctggctca aaacgcaatt ggggctgctt ggacatggtg cgagcatatt atttttcaga
     7981 ccaagattat gcggacgacc aactgaataa ccattaatac cccacaacat aattactcct
     8041 cctatcaaga tagatatttt tttttgagta aattgaaatt tattcggtct tttcataaat
     8101 catttcatga tatttgatga aataaaaata atcatgaagt attgaaaaac atttttacca
     8161 cagag
//
LOCUS       DTVD01000141            8160 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2466_length_8160_cov_361.454781, whole genome
            shotgun sequence.
ACCESSION   DTVD01000141 DTVD01000000
VERSION     DTVD01000141.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_8160_cov_361.454781"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(95..781)
                     /gene="rsmG"
                     /locus_tag="EYO68_04960"
     CDS             complement(95..781)
                     /gene="rsmG"
                     /locus_tag="EYO68_04960"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:PF02527.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIC07191.1"
                     /translation="MSEIQKPLKSIEVRQICLENDLEISDSQWQLLEKWAVMLLDVNQ
                     KVNLISRKETDLLWEKQILPCLSLLVLRKIEKGADVCDFGTGGGLPGMILAIVRPDLR
                     LTLLDSRQKKIVVLQQMIDALKLKNVQTVPGRGEELGKQRPWRHRFPFVTARAVAPLI
                     DLVRWTSDLRKPDSVLHIFKGGEIRNEVTALTKVVSAIKVKKSLMVLKGYPHFAENQK
                     YLISLEFSSK"
     gene            complement(858..1361)
                     /locus_tag="EYO68_04965"
     CDS             complement(858..1361)
                     /locus_tag="EYO68_04965"
                     /inference="COORDINATES: protein motif:HMM:PF02623.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HIC07192.1"
                     /translation="MLVQTSRFGEIEVKDDQIISFPSGLIGFSEDHRFVIREDEAAAP
                     FRWLQSIDSEDLAFVMIEPHVSISNYELELTNDHLKKLHAEKTEDLSVYSLVTMAKKM
                     EDVTINLQGPLLFNLEKRLGIQIIVQDGRYSTRHSLFAEKLKKSTPESSSQEEPENSS
                     KSLEQAG"
     gene            complement(1376..1606)
                     /gene="csrA"
                     /locus_tag="EYO68_04970"
     CDS             complement(1376..1606)
                     /gene="csrA"
                     /locus_tag="EYO68_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIC07193.1"
                     /translation="MLILTRKSGESITIGDDVKITVVEVKGKQVRIGIDAPRSYIIHR
                     EEVYISIQEENRRAAEESPLSLAGIKHLFGKL"
     gene            complement(1675..2976)
                     /locus_tag="EYO68_04975"
     CDS             complement(1675..2976)
                     /locus_tag="EYO68_04975"
                     /inference="COORDINATES: protein motif:HMM:PF00700.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07194.1"
                     /translation="MRVTEVTKRDHVVDNIQRSSGKLQDIQIQMASGRRLNKTSDDPI
                     GAARSQDIVTTLSSQKQQLQNIEDNIAWLQRSELEIGHINEILGQMRTLAISQAGSDS
                     NEETRQMVAREFAVARKTLFDTGNAREGKLYLFSGIKSLSPALKKNGIFQPAKVEKSG
                     VAQKDIRELLDVKQFRAQFEGFSTNNYRIKVTKGGIWGQARIKISDDGGRTWSKEQTL
                     RPVIHVFNQDGKLNDQVLLKFSDQEGRLGNILPKQFDFNSEKSAKFDLDALGVLFPEG
                     IEFVYQPNPEVSYNGSIHKKESLISNGLSIPVNITAQELLLGEGEDGVDTFSLLAAME
                     RALLENDGIAIAKRIDKLNLAQNQVLKQQADVGNIVRELYSTQSRLEDQQFEKERQLS
                     DIQDLDVAEATVDLKVAEANNILSLNTGARLIQPSLSDFLR"
     gene            complement(3056..4747)
                     /gene="flgK"
                     /locus_tag="EYO68_04980"
     CDS             complement(3056..4747)
                     /gene="flgK"
                     /locus_tag="EYO68_04980"
                     /inference="COORDINATES: protein motif:HMM:PF06429.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HIC07195.1"
                     /translation="MSSLNSSLNLGQRALSINQRAMQTVGHNIANQETEGFSRQQVRS
                     GTSAPDPTGVGGGADAQPTTRVYDKFVQRKILQENPRSGMFKSRGEFLQKIEIIFSET
                     EGNGLHQALNEFWNSWSQLSNQPESESARMQVKVHSDVLARRFRNMHSQLDGLRKEIN
                     GRLNANINKVNELGQKVAELNRQINLYEGGGQRNANDMRDARNQAIEELSDLVDINSF
                     EDPNGRTTVIIGRDWTLVEGNNRYQLEGTMKGGELGMLRVDGVSTNDNRRDLTRTFRE
                     GEMSEMLRMRDETIVSYQHNLDEIAFSLAGKVNRIHATGTGINSAAEMMKSTFGLNPD
                     ALNQPLPFLKDGIFQLHLVDPHNEILETYEIEIQAGKDSLPDIVQRLNQTINDPGLLQ
                     ASIESDGSLLLQSAPDYKFIFGEDQSSITQVLGLNSFFDTLKGAEDIQLSEHIRENTN
                     NISTGKDLIPGDNRVALEIAKLQTRPTMRDETMTFDEYYNGVLTGMGLKIQRNKTEQA
                     QQESMVRQFKEIRSSISAVNMDEELTDMMQYQKAYEASARFISTVDKMMETVINM"
     gene            complement(4801..5283)
                     /locus_tag="EYO68_04985"
     CDS             complement(4801..5283)
                     /locus_tag="EYO68_04985"
                     /inference="COORDINATES: protein motif:HMM:PF05130.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HIC07196.1"
                     /translation="MQELLNIMRQEVELHEQLISQLQKESEGFGRLRGSELLKLQGEK
                     SRCVRATARLERERIQFVEKLADSWNTKSKELTLSVIIDRTEDEFSKPLQQCFERLKS
                     LVAEIRKIADENALQASGRLKSVESSIRFMSQLQNGPPTYSDAGKLQNGTSTMSRTEA
                     "
     gene            complement(5347..5643)
                     /gene="flgM"
                     /locus_tag="EYO68_04990"
     CDS             complement(5347..5643)
                     /gene="flgM"
                     /locus_tag="EYO68_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HIC07197.1"
                     /translation="MKITGQQPPEFHGVKGGTAKDSQKTADRSGLTSGTSEPPVKTST
                     FVMNKIKDRISTEPEVRADRVAELKDKIKNGEYQVDAQRLAKTMLEDALQEDIS"
     gene            complement(5967..6287)
                     /locus_tag="EYO68_04995"
     CDS             complement(5967..6287)
                     /locus_tag="EYO68_04995"
                     /inference="COORDINATES: protein motif:HMM:PF10135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07198.1"
                     /translation="MKVSMPLPLSVLTLGADRFKAAPEIKGVTDDAKLRETASEFEAI
                     FIQQMLKTMRESSFESDLLPKSEGEKMFQSLLDEQYAKLSAKSGSLGLGEMIYQQLKS
                     NLDK"
     gene            complement(6294..7418)
                     /locus_tag="EYO68_05000"
     CDS             complement(6294..7418)
                     /locus_tag="EYO68_05000"
                     /inference="COORDINATES: protein motif:HMM:PRK05303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HIC07199.1"
                     /translation="MTMKNQLPFLRLGTALLYFFLLAVLTTPAWGVRVKDIAALRGAR
                     DNELIGFGIVVGLDGTGDSQESLLTRKPIVNALERIGISLKSQDILGRSIAAVWLTAT
                     LPPFAKSGQRLDITAATIGDAVSLRGGVLIMAPLRGPDRLVYALGQGPIAGIPKGVSR
                     ADALPAEELANLSIGSRMVASVGHVHGGAIVEREISLNLNSRARLYMNLHSPDFTTAF
                     RLAKLINQNLGIRSARAQDAGTVEVSVPDSYLGNTVELVSFIENLEITPDHTAKVVLD
                     ERSGTVVMGGSVRISPIAISQNGLNIQVKLPTLNVEGTQGELTEGRILASSVFMLKGG
                     TDLKEIVDGFNKIGASSKDLIEVLKAVKTAGALHAELVIR"
     gene            7953..>8160
                     /locus_tag="EYO68_05005"
     CDS             7953..>8160
                     /locus_tag="EYO68_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07200.1"
                     /translation="MEMILLFFCLTSVVITSVIVALELRGDKTLSRQSYKGEMNTINF
                     HGQQILAKDKNTNEQLRNRVFETLD"
BASE COUNT         2185 a         1888 c         1568 g         2519 t
ORIGIN      
        1 cgatataagt attttatcag atccctctct tcggtcgaga tgacaaaccg taaacaacaa
       61 tatcattatc ttacgagaac tcgtgactaa cgagttattt tgaggaaaat tctaaagata
      121 ttaaatattt ttgattctct gcaaaatgtg gatatccttt tagtaccatc agagacttct
      181 tgactttgat tgcggaaact actttagtca gtgccgttac ttcattcctt atttctccac
      241 ccttaaaaat atgcagtact gaatccggtt ttctcaaatc agaggtccag cgtactaaat
      301 cgatcaaagg tgcaactgca cgtgctgtca caaacgggaa tctatgacgc cagggacgtt
      361 gtttgcctaa ctcttcccct cgcccaggga cagtctgaac atttttgagc tttaatgcat
      421 caatcatttg ttgaaggacc acaatttttt tctgacgtga atccaacaaa gtaagccgca
      481 aatcaggacg tactatggcc agtatcattc ccggcaatcc tcctcctgta ccaaaatcac
      541 aaacatctgc acctttttca attttacgta aaaccagtaa tgaaagacag ggcaatattt
      601 gtttttccca caataaatct gtttctttgc gggaaattaa atttactttc tggttgacat
      661 ccagcaacat aacagcccac ttttccagca gctgccattg ggaatctgaa atctccaaat
      721 cattctctaa acagatttgt ctgacttcaa tagatttaag gggtttttgt atttcactca
      781 ttattgagtg tttgcaggaa ggaattttgt cagcatgaga acttcagttt cattgccgat
      841 tacgggctga tgcaggatca cccggcttgt tctaaagact tgctgctgtt ctccggctct
      901 tcctgagatg aagactcagg agtactcttt tttaattttt cagcaaacag tgaatgacgg
      961 gtagagtatc taccatcttg aacaatgatc tgaattccaa ggcgcttttc aagattaaaa
     1021 agcagggggc cctgtaaatt tatagtcaca tcttccattt ttttggccat ggtgaccagg
     1081 ctatagactg acagatcttc agttttttct gcatgaagtt ttttcaggtg atcatttgtt
     1141 aactcaagct cataatttga aatagagaca tgcggttcaa tcatcacaaa agcaagatct
     1201 tcactatcaa tggactggag ccagcgaaaa ggtgctgctg cttcatcttc ccggatcaca
     1261 aaacgatggt cttccgaaaa acctatcaat cctgaaggaa agctaattat ttgatcgtct
     1321 ttaacttcga tctcaccgaa gcgtgaggtt tgaacaagca ttaatgtttt attgattaga
     1381 gtttaccaaa aaggtgtttg attccggcta acgaaagtgg tgactcttct gccgcacgcc
     1441 tgttttcttc ctgaatgctt atatatactt cttctctgtg gattatataa cttcggggtg
     1501 catcaattcc aattcgaact tgtttgccct tgacctcaac caccgtgatt tttacatcat
     1561 ccccaatggt tatactttct cccgacttcc ttgtcagtat gagcataatg atctgtcttc
     1621 ttgataatta attccaaatt atacaaaacc tgtgattatc attcacaggt taaattaacg
     1681 gagaaaatcc gacagtgacg gttggatcaa gcgggctccg gtattcagcg aaagtatgtt
     1741 gttagcctct gcgactttaa ggtccacagt tgcttccgca acatctaaat cctggatatc
     1801 agaaagttgt ctctcctttt caaactgctg atcttccagc ctggactgag ttgaatagag
     1861 ttcgcgaaca atatttccaa cgtctgcctg ctgcttcaaa acctgatttt gtgccaagtt
     1921 aagcttatct atacgttttg caatcgcaat gccatcattt tcgagaagag ctctctccat
     1981 tgccgcaagt aaagaaaacg tatccacgcc atcctcacct tctccaagca gcagttcctg
     2041 agcagtaata tttaccggaa ttgaaagtcc atttgaaatc aaagattctt ttttatgtat
     2101 tgatccatta tatgatactt ccgggtttgg ctgatacaca aactcaattc cttccggaaa
     2161 caaaacacca agtgcatcca agtcaaattt tgccgatttc tctgaattaa aatcaaattg
     2221 ttttggcaga atgttgccca aacgaccttc ctggtcagaa aatttgagca aaacctggtc
     2281 gttaagtttt ccatcctggt taaatacgtg aatcaccgga cgtaatgtct gttctttact
     2341 ccaggttcgt ccaccatcgt ctgaaatctt tatcctggct tgtccccaaa ttccgccttt
     2401 tgtgactttg attcgataat tattagtaga aaaaccctca aattgcgctc taaattgttt
     2461 cacatccagc aattccctga tgtctttctg cgcaactccg cttttctcaa cttttgccgg
     2521 ttgaaatata ccattttttt tcaaagcagg agaaagactt tttatgcccg aaaacaaata
     2581 aagttttcct tcacgcgcat ttcctgtgtc aaataaagtt ttccgggcca cagcaaactc
     2641 tcgagccacc atttgcctcg tttcttcgtt tgaatcactg ccggcctgtg agatagcaag
     2701 tgtgcgcatt tgtcccaata tttcattaat atgtccaatt tccaactccg aacgctgcaa
     2761 ccaggcaata ttgtcttcaa tgttctggag ctgctgtttc tgtgaagaaa gtgtagttac
     2821 aatatcctgt gatctggccg caccaattgg atcatccgag gttttgttca aacgacggcc
     2881 ggaagccatc tggatttgaa tatcctgaag cttccctgaa gatctttgaa tattgtctac
     2941 tacatggtca cgcttagtga cctcagttac acgcatttaa aggacttgtg gtttaagttt
     3001 ttgacaaaag tcttgggttg cctcttactt tcttctgttt cttttgcttg attcattaca
     3061 tattaataac ggtttccatc attttatcta cagtactgat aaaacgtgca gatgcttcgt
     3121 atgctttctg atactgcatc atatcagtca gttcttcgtc catattgact gcactgatag
     3181 agctcctgat ttccttaaac tgcctcacca tactttcctg ttgtgcctgc tccgttttat
     3241 ttctttgaat tttcaaaccc attcctgtga gcacaccatt ataatactca tcaaaagtca
     3301 tggtttcatc tctcatagtc ggacgtgtct gcagcttcgc aatttccagt gcaacccggt
     3361 tatcaccggg aataaggtcc ttgcctgttg aaatgttatt cgtgttttca cgaatatgct
     3421 cacttagctg gatatcttct gctcctttga gtgtatcaaa aaaactattc aaacccagaa
     3481 cctgtgtgat gctggattga tcttcaccaa aaatgaactt gtagtccggt gcactttgta
     3541 aaagcagcga gccgtctgac tctatggaag cctgtaacaa tcccggatcg ttgatggttt
     3601 gattcaggcg ttggacaata tcgggtaagg aatctttccc cgcctgtatc tcaatttcat
     3661 aagtttccag aatttcatta tgaggatcaa ctaaatgaag ctgaaatatt ccatctttca
     3721 ggaaaggcag gggttgatta agtgcatccg gatttaagcc gaaagtactc ttcatcattt
     3781 ctgcggcaga atttattcca gtacctgttg cgtgaatcct gttaactttt cctgcaagac
     3841 tgaaggcgat ttcgtcgaga ttatgctggt agctcacaat tgtttcatcg cgcattctta
     3901 acatttcact catttctcct tctcgaaaag tgcgggtcaa atcacggcga ttgtcatttg
     3961 tcgaaactcc atcgactctg agcataccca attcaccgcc cttcattgta ccttcaagct
     4021 gatagcggtt atttccttct accagagtcc agtcacggcc gatgataact gtagttcttc
     4081 cgttgggatc ttcaaaagag ttaatatcca ccaagtctga caactcctca attgcctgat
     4141 tgcgggcatc cctcatatcg ttggcattcc gctggccgcc gccctcgtat aagttaatct
     4201 gtctattcag ctcggctact ttctggccca actcatttac tttatttata tttgcattca
     4261 gacggccgtt aatttcttta cgcagcccgt caagttgaga gtgcatgtta cgaaagcggc
     4321 gtgccagcac atcactatgc acctttacct gcattctggc tgattcagat tccggctgat
     4381 tcgaaagctg gctccatgag ttccaaaact cgtttagagc ctggtgtaag ccgtttccct
     4441 ctgtttcact aaaaataatt tcaatttttt gcaggaattc tcctcgagac ttaaacatgc
     4501 ctgaacgcgg attttcctgg aggattttgc gctgcacgaa tttatcataa acccgtgtag
     4561 tcggctgggc gtcggcacct ccccctactc cagtgggatc tggtgcagac gtgcctgagc
     4621 gtacttgctg gcgggagaaa ccttcagtct cttgattggc aatgttatgc ccaactgttt
     4681 gcattgcacg ctgattgata ctcagggcgc gctgtcccag atttagggaa ctattgagcg
     4741 aagacatgtg acaatgctga aatggttagg gttcctaaac gattttagtg acttataaag
     4801 tcaggcttcg gtacgggaca tggtgctggt accattttgc agtttccccg catctgaata
     4861 agtcggagga ccgttctgga gctgactcat gaaccgaata gaggattcaa cagattttaa
     4921 gcgacctgat gcctggagag cgttttcatc cgcaattttg cgtatttcgg caaccaggct
     4981 tttcaaccgt tcaaaacatt gttgaagtgg ttttgagaat tcatcctcag tcctgtcaat
     5041 aataacgctt aaagtcagtt cctttgattt agtgttccag gaatctgcaa gcttttccac
     5101 gaactgaatc cgctccctct cgagtctggc agttgcacgt acacaacgag acttttcgcc
     5161 ttgaagttta agcaactctg aaccgcgtaa acgcccaaat ccctcggatt ctttttgcaa
     5221 ctgtgaaatc aactgctcat gcagttccac ttcctgtcgc ataatattca ataattcttg
     5281 catcttaaaa ggacaccatc taacggactt agttaggccg ccgtgttatt tttcagcact
     5341 taagattcag cttatatctt cctgaagagc atcctccagc atcgttttgg caagacgttg
     5401 cgcatcaacc tgatattcac cattttttat tttatcttta agttctgcaa cccggtctgc
     5461 acgtacttcc ggctcagttg aaatcctatc ttttatttta ttcatcacaa atgtcgaagt
     5521 cttaactgga ggttctgatg ttccggatgt caatcctgaa cggtctgcag ttttctgact
     5581 gtctttagca gtccctccct tgacaccatg aaactcgggg ggctgttgac ctgttatttt
     5641 cataattcac ctgtcaatgt taaatagttt agatccctat ctctgcggga atgtccggtg
     5701 taagccgaca agcatacccg cagactctga cgagcacaac taaaaatgtg cagttcgcta
     5761 gcctgagaag gcccataggg cgtgtaaact aacatgattc aagaagcgtt tgcttccgaa
     5821 aatgcgtata cagacacttc agtcttgggc ctcaggtcag atgttaccgg gtctggaaag
     5881 gccatatatt tgctttcctg acaagtcaca gaaacacctt attgcatgta tcggccaatc
     5941 aatcacaaaa ctttagcgtt tttttattat ttgtctaaat tcgatttcaa ctgctgataa
     6001 atcatctctc ccaggcccag acttccggac tttgcagaaa gtttcgcata ttgttcatcc
     6061 agtagagatt ggaacatttt ttcaccttct gacttaggaa gcagatcaga ctcaaaagat
     6121 gattcccgca ttgttttcaa catctgctgg ataaaaatcg cctcaaactc actggcagtt
     6181 tcacggagtt ttgcatcgtc ggtgactcct tttatttcag gagccgcctt aaatcgatcc
     6241 gcacctaagg tcagtacaga taaaggtaac ggcattgata ctttcatttt cgtttatcga
     6301 atgacaagtt ctgcatgaag agcgccggca gtttttacag ccttgagcac ctcaattaaa
     6361 tctttgctgg aggcgcctat tttattaaag ccatccacaa tttccttcaa gtcagtacct
     6421 cctttaagca taaaaacact ggaagcaagt attcttcctt cagtcagctc accctgggtt
     6481 ccttctacat ttagagttgg aagtttaact tgaatattca aaccgttttg agaaattgca
     6541 attggcgaaa tcctgacact gcctcccatc actacagttc cagaccgttc gtcaagaaca
     6601 actttagcag tatgatctgg agtaatttcc aaattttcaa tgaaggaaac caactccacc
     6661 gtattgccca agtaactatc cggaactgag acttcgacgg tgccggcatc ctgggcacgg
     6721 gcactccgga tacctaaatt ctgattaatc agctttgcca aacggaatgc tgtagtaaag
     6781 tccggagaat gcaggttcat atacagtcgg gcccggctgt taagatttaa actaatctca
     6841 cgttctacaa tcgctcctcc gtgaacgtgc ccaactgagg ccaccatccg tgatcctatc
     6901 gacaaattag ccaattcttc tgcaggcagt gcatcggctc tgctgactcc tttcggtatt
     6961 cccgcaatgg gaccctgccc gagggcataa acaagtctat ccggtccccg taacggagcc
     7021 attatcaaaa ctcctccgcg aagtgaaacc gcatcaccaa ttgttgcggc agtaatgtct
     7081 aaacgttgtc ctgattttgc gaaaggtggt aaagtcgctg tcagccatac tgcagcaata
     7141 cttcgtccta aaatatcctg gctctttaag ctaatcccaa tcctttccaa ggcattcaca
     7201 atcggctttc tggttagtaa actctcctgt gaatcgcctg tgccgtccaa accaactaca
     7261 ataccaaagc cgataagttc gttgtcacgt gcaccccgca atgctgcaat atcctttacg
     7321 cgcacacccc aggctggtgt tgtcaaaacg gccaacagga agaaatacag caatgcagta
     7381 cccaatcgta aaaaaggcag ttggtttttc atggtcataa aaaatgaatg ttcttcaaga
     7441 ttttggtgtc cagtttgtaa tacaagttca atgccagatt aatgcagttc tgctattcag
     7501 tttgcgctca ttaactgcat caatcacatg agtataattc cggttaaaac cgccttgaat
     7561 tgtgcattga agaaaaagat tcagcttcat aaaatgtcgt cgtttaaaaa caatgggaac
     7621 atatttgagg aaatgctgat tgacatcagc tattttttct atcaattttg atgaatctgc
     7681 aaaaagtcct aaaaccgtgt cattccgagc acagcgagaa atctctcata aactacttca
     7741 ctaatatcac aaagatttca gactttttgc gaagccatta attttttgct ggtctgtttt
     7801 ctgcattgtc tccgggagaa gaaatttgag ctagttgaat ctataaaatt gcacagcact
     7861 tggtccgaat cggaatctaa ggaatttgag ataaaattag aacggacttt tgaaccctgt
     7921 aaaactagta aaaaccagca aatgaggtta ctatggaaat gattttattg tttttttgcc
     7981 tgacttcagt ggtgatcact tcggtgattg tcgcattaga gttaagagga gacaaaactc
     8041 tgtcgaggca gtcttacaaa ggtgagatga acaccattaa ctttcacggg cagcagattc
     8101 tggcaaaaga taaaaacacc aatgagcagt tacgtaacag ggtgtttgaa accttggatc
//
LOCUS       DTVD01000142            2021 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_24763_length_2021_cov_241.412513, whole genome
            shotgun sequence.
ACCESSION   DTVD01000142 DTVD01000000
VERSION     DTVD01000142.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24763_length_2021_cov_241.412513"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="EYO68_05010"
     CDS             <1..1541
                     /locus_tag="EYO68_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIC07201.1"
                     /translation="EPRGMRDYTQDFIHYYKDIQKTGRDYHNENPDYFLQEIGKGQGS
                     DISIFLYTSGTTGKPKGVVLTSDNLIITARNSIEFDNLTSEEEVLAYLPMAWVGDNIF
                     SLAQAYVIGFCVNCPENPETVMTDLKEIGPTYYFAPPAIYETVLTKVMIRMEDASKIK
                     RSMFKYFMDLAKSVGIRILDGKPVSFKERILYSIGQFLVYGPLKNNLGLSRTRLAYTA
                     GEAIGPEIFEFFRSLGINIKQLYGQTEASVFICAQPDGQVKADTVGTAYPGVELRLAD
                     NNEVFYRSPGVFHSYYKNPESTAETKDAEGWVATGDAGFFDEDGHLKIIDRAKDVGRM
                     SDGTMFAPKYIENKLKFFPFIKEAVAFGDEKEFTTAFICIDIEAVGNWAERKNLAYSG
                     YTDLSARAEVYDLLQECVETVNADLARDEKLSGSQIKRFLLLHKELDADDGELTRTRK
                     VRRRIVAEKYAVLISALDDPQQTHCEIDSQMTFEDGRTGMVHADLQIRESALAESQVH
                     TLAA"
     gene            1570..>2021
                     /locus_tag="EYO68_05015"
     CDS             1570..>2021
                     /locus_tag="EYO68_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC07202.1"
                     /translation="MESKFGDYLLSVDHISLSFGGVKALKDISFGIREKEVFAIIGPN
                     GAGKTSMLNVINGFYHPQEGEITYKGVHRKQMKSHDAAVHGIARTFQNVALFKGMSTI
                     DNIMTGRIIHMKRNLFWQAFYLGPARKEEMANREYVEDIIDFLEIQAIR"
BASE COUNT          606 a          407 c          463 g          545 t
ORIGIN      
        1 aggaaccccg cggaatgcgt gactacaccc aggattttat acactactac aaagacatac
       61 agaaaacagg acgtgattat cacaatgaaa atcctgatta tttcctccag gaaattggga
      121 agggacaagg tagtgatatt tccattttcc tttacacttc cggaaccacc ggaaaaccaa
      181 aaggcgtagt tctcacttca gataatctca tcatcactgc acgcaacagt attgaatttg
      241 acaatctcac cagtgaggaa gaggtgctgg cctatttgcc gatggcctgg gttggagaca
      301 acattttctc tttggcacag gcatacgtca tcggcttctg tgtaaactgt cctgagaatc
      361 ctgaaacagt gatgacagat ttgaaggaaa ttgggccaac ctattatttt gctccaccgg
      421 ctatttatga aaccgtgctg acaaaagtga tgatccgcat ggaagacgca agcaaaatta
      481 aacgctccat gttcaaatat tttatggatc tggcaaaatc tgtgggaatc cgtattctgg
      541 atggaaaacc agtctccttt aaagaaagaa tcctttacag catcgggcag tttttagttt
      601 atggtccctt gaaaaataat ttgggtttga gtagaacccg tctggcatac actgcggggg
      661 aagcaattgg tccggaaatc tttgagtttt tccgctctct tggcatcaat atcaaacaac
      721 tttacggaca aacggaagcc tccgtattta tctgtgcaca accggatggt caagtaaaag
      781 cggacactgt tggaactgct tatccgggag ttgaactgcg gctggcagac aacaacgaag
      841 ttttttaccg aagtccgggg gtttttcatt cttattataa aaatccggaa tctactgcgg
      901 aaactaaaga tgccgaaggc tgggtggcaa ccggagacgc aggatttttt gatgaggatg
      961 gacatcttaa aatcatcgat agagcaaaag atgtgggcag gatgtctgat ggaaccatgt
     1021 ttgcgcccaa gtacattgaa aataaactca agtttttccc ttttatcaag gaagcggttg
     1081 cttttggaga tgaaaaagaa ttcaccactg cgttcatttg tattgacata gaggcagttg
     1141 gtaactgggc agaacgtaaa aatttggctt actcaggtta tacggatctt tctgccagag
     1201 cggaagtata tgatctgctg caggaatgtg ttgaaactgt taacgctgat ttggcccgtg
     1261 atgaaaaact cagtggttca caaataaaaa ggttcctgtt gttgcataaa gaattggatg
     1321 cagatgacgg tgaactcaca aggacccgta aagtccgccg gagaatcgtt gcagaaaaat
     1381 atgctgtctt aatctccgcg ctggatgatc ctcagcagac tcactgtgaa attgattcac
     1441 aaatgacttt tgaggatggc cgtaccggaa tggttcacgc agatttgcag attcgggaat
     1501 cagcactagc tgaatcacaa gtccacactc tcgccgcata aactcctaat accaaataaa
     1561 atattgtcca tggaaagtaa atttggggat tacctgttga gcgtcgatca tatcagtttg
     1621 tcatttggag gagtcaaagc tttaaaagac attagttttg gaatacgtga aaaggaagta
     1681 ttcgccatca tcggtccaaa tggtgccggt aagacatcca tgttaaatgt gataaatggc
     1741 ttctatcatc cccaggaagg cgaaattaca tacaaagggg ttcatcgtaa gcaaatgaaa
     1801 tcccatgatg ccgccgtcca tggaattgcc cgtactttcc agaatgttgc tcttttcaag
     1861 ggcatgtcca ctatcgacaa tatcatgact ggccgtatca ttcacatgaa acgaaacttg
     1921 ttttggcaag cattttatct gggacctgcc cgtaaagagg aaatggcaaa tcgggaatac
     1981 gttgaggaca ttattgattt tcttgaaatt caagccattc g
//
LOCUS       DTVD01000143            1997 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_25225_length_1997_cov_280.570031, whole genome
            shotgun sequence.
ACCESSION   DTVD01000143 DTVD01000000
VERSION     DTVD01000143.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25225_length_1997_cov_280.570031"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(26..874)
                     /locus_tag="EYO68_05020"
     CDS             complement(26..874)
                     /locus_tag="EYO68_05020"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIC07203.1"
                     /translation="MTAQKIIQIRDVKLSNENSFALVGGLNVLESESVALQVAETFRS
                     ITDKLRIPYIFKASFDKANRSSIDSFRGPGLDEGLQILEKIKSEFDVPILTDIHEPYQ
                     AAPAADVADVLQLPAFLSRQTDLIVAAAKTGAAINIKKAQFLAPEEMSNILQKCESSG
                     NDRLILCERGTSFGYNNLVVDMLGFSVMKSFGYPVLFDVTHALQRPGGMGDAAAGRRE
                     SVRELALAGMSQKIAGLFLEAHPEPEQALCDGPCALRLNQLEPFLKQMRAVDDLVKSF
                     EPLDTA"
     gene            complement(883..1932)
                     /locus_tag="EYO68_05025"
     CDS             complement(883..1932)
                     /locus_tag="EYO68_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HIC07204.1"
                     /translation="MGLLTTTIGAYPKPEYVKLPDWFGNLDTSDPTRGWAEALNAMGD
                     DVNSILERGTHEAVNDQVDAGIDIPTDGEITRENYIHYHCRHLEGMDFENLTEKTLRT
                     GNYSSLLPTVRGPVKTRDLFLADDWRRAQEATDKPVKITMPGPLTVADTVVDEYYGNQ
                     KDFGKAIAEALNQEVLSLANAGCRHIQIDEPLFARYPERALEFGVENLERAFQNCPDG
                     VTRTAHMCCGYPDVIDAVDYPKAPRESYFQIADAMEDSSVMALSLEDAHRYNDLSLLE
                     HFKTTTIIFGVVAIAKSRVEAVEEIRKRLMDALENIDANRLIAAPDCGLGILGRELAV
                     QKMKNLCEAAHSIET"
BASE COUNT          525 a          467 c          423 g          582 t
ORIGIN      
        1 tctccaaaat catttccact agttgttaag ctgtgtccaa aggttcaaaa gattttacta
       61 aatcatctac agccctcatt tgcttcagga agggttcgag ctgattcagt cggagtgcgc
      121 agggaccgtc acaaagcgct tgttccggtt ccggatgtgc ttccagaaaa agacctgcga
      181 ttttttgaga catacctgcc aaagcaagtt ctcgcacaga ttcccgccgt ccagctgctg
      241 cgtcacccat acctcccgga cgctgaagcg catgagtaac gtcaaaaaga actggataac
      301 caaaactttt catcacagaa aagccgagca tatccaccac caaattgttg tagccgaaac
      361 ttgtgccgcg ttcgcaaaga atgagccggt catttcctga ggattcgcat ttctgcagaa
      421 tgttactcat ttcttccggg gctaaaaact gggctttttt aatgttgatt gcggccccgg
      481 ttttagccgc tgcgactatc aagtcggttt gacgagacaa aaaggcagga agctgcaaaa
      541 cgtctgcgac atctgcagct ggtgccgcct gataaggctc atgaatatca gtgaggatgg
      601 gcacgtcaaa ttcggattta attttttcga gaatctgtaa accttcatcc agtcccggtc
      661 ctcgaaaaga gtcgatggat gaacggttgg ctttatcgaa ggaagctttg aaaatataag
      721 gaatccggag cttgtctgta atcgaccgga aagtttccgc tacctgtaaa gctacggatt
      781 cagattcaag cacgttcagt ccgccgacta gagcaaacga attttcgttg ctcaatttta
      841 catcacgaat ttgtattatt ttttgagcgg tcattttttg atttaagttt caatcgagtg
      901 tgcggcctcg cataagtttt tcattttttg gacagcaagt tcacgtccca aaattccaag
      961 tccgcagtcg ggtgcggcaa tcagacggtt tgcgtcaata ttttccagtg catccattaa
     1021 acgcttccga atttcctcga ctgcttcaac gcgacttttt gctatggcaa ctactccaaa
     1081 aataatggtt gtggttttga aatgctccag caaactcagg tcattgtagc ggtgtgcatc
     1141 ttccaaagac agggccatga cagacgaatc ttccattgca tccgcaattt ggaagtatga
     1201 ctcacgagga gctttaggat aatcaactgc atcaattaca tcaggatatc cacaacacat
     1261 gtgagcagtt cgggtaactc cgtccggaca gttttgaaat gcacgttcca aattttcgac
     1321 tccaaattca agcgcacgtt caggataacg ggcaaaaagg ggttcgtcta tttgaatgtg
     1381 ccggcagcct gcattggcca gactgagtac ttcctggttc agtgcttcag caatcgcttt
     1441 cccaaaatct ttttgattac cgtaatattc atccacgacg gtatctgcaa cagtcaaagg
     1501 tccgggcatt gtaattttta ctggcttgtc tgttgcttcc tgggcccgcc tccaatcatc
     1561 tgctaaaaac aaatcccttg ttttgactgg accacgaacc gttggcagta acgaggagta
     1621 atttccagta cgcaaagtct tttctgtaag attctcaaaa tccattcctt ccagatgtct
     1681 gcaatgatag tgaatgtaat tttctctggt aatctcacca tctgtgggga tgtcaattcc
     1741 tgcatcgacc tgatcgttga ctgcttcgtg agttcccctc tcgagaattg aattaacatc
     1801 atcacccatt gcattcagtg cttcagccca acctcgggtc ggatccgatg tatccagatt
     1861 accgaaccag tctggaagtt ttacgtattc cggttttgga taagctccaa ttgtagttgt
     1921 taataatccc atattgtcat tttttgtaag atattaatta tcaaaaattt gtttcaaaag
     1981 tcttcaaaat ttacctg
//
LOCUS       DTVD01000144            8015 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2530_length_8015_cov_417.639824, whole genome
            shotgun sequence.
ACCESSION   DTVD01000144 DTVD01000000
VERSION     DTVD01000144.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_8015_cov_417.639824"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="EYO68_05030"
     CDS             <1..1215
                     /locus_tag="EYO68_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HIC07205.1"
                     /translation="ASENVQSDAVKQIESNDFMGRYAEGHPNTAQEDNRYYEGTRYID
                     QIESMTTREIIQLAKCLQADVRPVSGNAANTAVALGILRGGDTVLVNSIEIGGHISHN
                     PIGVVGRRIQVRGKVLTPGRENSISLHFWPATEDGYHLDVPKCVDLVEQTSPNMVILG
                     KSLFLFPEPVKQIAEVCREKNIPLLYDGAHVLGLILGGQFQNPFEEGAHFINGSTHKT
                     FPGPQRGVILGNMATEQEMKWWTSVDRGVMPGSSSSHHLHTLPGMLIAIREMAQFGRE
                     YAAQTVANAKALGQALTDEGVNVEAKEFGFTESHQLAINVTNFGIAKELARSLSDKNN
                     IITNYNMLPGDKDAKNPTGLRIGVQEMTRYGMKEDEMGELADLMKAGLQGKIVKDEVI
                     KLRSRFTDVHFA"
     gene            complement(1355..2731)
                     /locus_tag="EYO68_05035"
     CDS             complement(1355..2731)
                     /locus_tag="EYO68_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIC07206.1"
                     /translation="MSSPHSVVAIVGRPNVGKSTLFNRLVGRRSAIVEKNPGVTRDRN
                     SGISKYHGFQFIAVDTGGFEPVAESSLVQKMAIQARLAVEEADCVLFVVNALEGWTPA
                     DSEIYRTLVKAETPLFVVVNKADNPRLEEESFEFHQLGVENIYPISAEHNRGIEGLLE
                     EVDQLIPLKIKEKEDYNEDLSRSDAPIAVAVIGKPNAGKSSIVNALLRKERMIVDPVS
                     GTTRDPVDSQCSFEGRDILLVDTAGIRRRGKVSRKIETFSIVSALKSIERADVTLLII
                     DAEEGVTEQVTKIAGYANERRKSLVIVMNKWDLVKKDGITQKEVKEEVYDSLGFINFA
                     PVIFVSAVTGQRVTQIFENILRVHEQYVRRIQTSDLNTILQLIVNQHPPPSKSGRPTK
                     VFYGNQVSVAPPTFVFMTNNPEKTNFAYERYVSNQFRFHFGFEGTPLNFIWRKKKSTH
                     SKKPGKRS"
     gene            complement(2750..5152)
                     /gene="gyrB"
                     /locus_tag="EYO68_05040"
     CDS             complement(2750..5152)
                     /gene="gyrB"
                     /locus_tag="EYO68_05040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIC07207.1"
                     /translation="MPEIETVDKSTYDESDITVLQGLEAVRLRPGMYIGGVDSTALHH
                     LVFEVVDNSVDEALAGYCSEIHVIINSDGSLSVEDDGRGIPVGIHEEEGIPAVELILT
                     RLHSGGKFDNSNYKVSGGLNGVGASVVNALSGKMIAEIHREGFLWKQEYQQGITASSL
                     EQIEESKKTGTHITFWPDKTIFDNVDFNFEILAHRLRELAFLNKGILISIRDNRSDRF
                     QEFKYEGGIAAFIEHINENKNVLHESPVYFSGKSEDVEIEVAFQYNDSYTERIYSFVN
                     NINTVDGGTHLTGFKGALTRTLNSYMSANKITNDLEENLTGEDVREGMAAVISTRVTN
                     PQFESQKKIKLTNVEIKGKVETLVSEYLGAHLEENPAVAKKIVAKAIDAQRARLAAKK
                     AREITRRKNVLEFSTLPGKLADCQESDPALSELFLVEGDSAGGSAKQGRDRKNQAILP
                     LKGKILNVEKARFDKMLSHEEIKSMITAMGTGIGKEEFDLEKIRYHKIIIMTDADVDG
                     LHILTLILTFFYRQMPEIIENGYLYIAQPPLYRAKKGRSSFYAKNENELTERLVRASS
                     ETLSLTSEKGTTISGEDLFQTALKIRLYRTHYGRLCFNPNLTLLINLLLKHEIEIDLG
                     GAEHILNCIDNLKNSYPDYKLTVDPEKQGSNVLIQVNDTQIELSLNLLENLSAYDYSQ
                     LLQEHLNLKTALGENGVVLTEENEGESHSIQTWSQVLEFMVSFGKKGMYIQRYKGLGE
                     MNPEQLWETTLDPTIRTLKKVKVEDIVESDGIFTILMGDQVEPRRNFIVENALRVKNL
                     DV"
     gene            complement(5200..6267)
                     /gene="recF"
                     /locus_tag="EYO68_05045"
     CDS             complement(5200..6267)
                     /gene="recF"
                     /locus_tag="EYO68_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HIC07208.1"
                     /translation="MIIRNIEVSQFRNYSRCTTKLSPEINWIYGANGQGKTNLVEAVF
                     YLCNLESFRTTKPSLLLQEKKSEAVLQAKVERKKVLHLVRVNITKKGRQVFLDHKPSR
                     RVSEYIFSFMALSFTPEDVNLFRSVPQERRKFFNRIMAFNDPLYFNNIQEYSKIISQK
                     NALLRQGKIEQIQLWNEMLAKSAVQLMKHRNSYVEQVNQHLNDIFKDISGRTETLKLI
                     YKPSLNSADYEEKNCLQQLEKSLSRDLQYGFAVLGPHRDEYHLMMDGKKDRDFFSQGE
                     FRITNLSLKMTINRLFYEGYQFYPVLIFDDLFSELDDEVIKNVFQYFLTLKNQIFITS
                     TSEPSGSLTVKKFNIVKGELV"
     gene            complement(6345..7472)
                     /gene="dnaN"
                     /locus_tag="EYO68_05050"
                     /pseudo
     CDS             complement(6345..7472)
                     /gene="dnaN"
                     /locus_tag="EYO68_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
BASE COUNT         2333 a         1733 c         1537 g         2412 t
ORIGIN      
        1 gccagcgaaa acgtgcagtc ggatgcagtc aaacaaatag agtcaaacga tttcatgggc
       61 cgctatgcag aaggacatcc gaatactgca caggaagaca accgatatta cgaaggtacc
      121 cgctacatcg atcaaattga aagcatgacc actcgtgaaa tcatccagct ggcaaaatgt
      181 ctgcaggctg atgttcgtcc agtcagtgga aatgcagcaa atacagccgt ggctttagga
      241 atattacgcg gcggagatac ggtgctggtc aattccatcg aaatcggcgg acatatcagt
      301 cataacccga ttggcgtggt gggaagaaga attcaggttc gaggaaaagt actcactccg
      361 ggacgtgaaa actccatctc attgcatttc tggcctgcaa ctgaagacgg atatcatctt
      421 gatgtaccaa aatgcgttga cctggtggag caaacttctc cgaatatggt tattcttggc
      481 aaaagcctgt tcctctttcc ggaacctgta aagcaaattg cagaagtctg ccgggaaaaa
      541 aatattcctc ttctttatga cggtgctcac gttcttggtc tgattctcgg tggtcaattc
      601 caaaatccat ttgaggaagg cgcgcatttc attaacggaa gtacacataa aactttccct
      661 gggccacagc gtggagtaat tttaggcaac atggccactg aacaggaaat gaaatggtgg
      721 accagtgtag acaggggcgt aatgcctggt tcttccagca gtcaccatct gcacactctg
      781 ccgggaatgc tgatagcaat ccgggaaatg gctcagttcg gccgggaata tgccgcacaa
      841 acagttgcaa atgccaaagc tttaggacag gctctgactg atgaaggggt gaatgtggaa
      901 gccaaagaat tcggttttac cgaaagtcac cagttggcaa taaacgtaac caattttgga
      961 attgccaaag agcttgcccg ctcactctct gataaaaaca acatcatcac caactacaac
     1021 atgctgcctg gcgacaagga tgcaaaaaat ccaaccggtt tgcgaattgg tgtgcaggaa
     1081 atgacacgct acggcatgaa agaagatgaa atgggtgagc tggctgattt gatgaaagcc
     1141 ggactgcaag gaaaaatagt caaagatgaa gttatcaagc tgcgcagccg ttttaccgac
     1201 gtacactttg cctgacaatc aggttatgaa agaaaattca accagcacaa ttttaccagg
     1261 gtttcagtaa atttggaatg gccatggatt tagctgtcag ttttcagctg tcagtctgaa
     1321 aatcaggcaa aaatactgat cttggatatc tttttcagct gcgttttccg ggttttttac
     1381 tgtgagtcga ttttttcttg cgccagataa aattcaaagg agtaccttca aaaccaaaat
     1441 gaaagcggaa ctggttggaa acatagcgct cgtaagcaaa gtttgttttt tcgggattat
     1501 tggtcatgaa gacaaaagtc ggcggggcaa cactgacctg attaccataa aaaaccttgg
     1561 tcggccggcc cgattttgat ggaggcggat gttggttgac aatcagctga agaattgtat
     1621 tcaaatctga agtttgaatc cgccggacgt actgctcatg aacccgcaga atgttttcaa
     1681 aaatttgagt aacgcgctgt ccagtcaccg cactgacaaa aataaccggt gcaaagttga
     1741 taaaacccaa actatcgtaa acttcttctt tgacttcctt ttgggttatg ccgtcttttt
     1801 tgaccagatc ccatttgttc atcacaatca ccagtgattt tctgcgctca ttggcatagc
     1861 cggcaatctt ggtaacttgc tctgttaccc cttcttctgc atcaataatc aaaagggtta
     1921 catccgcacg ttctattgat ttgagtgcag aaacaatact gaatgtttca atcttccgac
     1981 tgaccttgcc gcgtcgacga attcctgcag tatcaaccag caaaatatcc cggccttcaa
     2041 aactgcactg actgtcaacc ggatcacggg ttgttcctga aaccggatcc acaatcatcc
     2101 gctccttccg gagaagtgca tttacaatcg acgattttcc tgcatttggt ttaccaataa
     2161 ccgcaactgc aataggagca tcagagcggc ttagatcttc attgtagtct tctttttctt
     2221 ttattttcag tggaatcagc tgatctactt cttccaaaag tccttctatt ccacggttgt
     2281 gctcagcaga aattggataa atattttcta ctccaagctg atgaaactca aaactttctt
     2341 cttccagacg ggggttatcg gctttgttga caacaacaaa caagggagtt tctgctttca
     2401 ccaatgtcct gtatatttca ctgtctgccg gagtccagcc ttccagtgca tttacaacaa
     2461 acaaaacaca atctgcctct tcaacagcca gacgtgcctg tattgccatt ttctgaacca
     2521 atgagctttc tgcaactggt tcaaatccac ctgtatcaac tgcaataaac tgaaaaccgt
     2581 gatattttga aattccggaa ttgcgatcac gtgttacacc cggattcttt tctacaatgg
     2641 cagaacgtcg cccgacaagg cgattgaata atgttgattt tcctacattt ggacgcccga
     2701 ctatggcgac aacggaatgg gggctggaca tggagttggt gcaataattt caaacatcca
     2761 aattcttcac tcgaagtgcg ttttccacaa tgaagtttcg gcgtggctca acttgatcgc
     2821 ccatcagaat ggtgaaaatt ccgtctgatt caacaatatc ttcaactttc acttttttaa
     2881 gtgttctgat tgtcggatca agtgttgtct cccatagctg ctccggattc atttcaccca
     2941 atcctttgta acgctgaatg tacatgcctt tcttgccaaa actaaccata aattccaaca
     3001 cctgagacca tgtctggata gagtgactct caccttcatt ttcttcagtc agtaccactc
     3061 cattttctcc aagtgccgtc ttgaggttca aatgttcctg taacaattgg gaataatcat
     3121 atgcgctcag gttttccaat aaattcagag acagctcaat ttgtgtatca ttaacctgga
     3181 ttagaacatt acttccttgt ttttccggat caacagtcag tttgtagtcc ggatagctat
     3241 tcttcaagtt gtcaatacag ttgaggatgt gctctgcacc tccaagatca atctcaattt
     3301 catgcttgag caggagattg atcaaaagag ttagattggg gttaaagcac aagcggccat
     3361 aatgggttct gtaaagcctg attttaagtg cagtctgaaa aagatcttct ccactgattg
     3421 ttgttccttt ttcagaagtg agacttagag tttcactgga agcgcgcacc agccgttctg
     3481 tcaattcatt ttcattcttg gcataaaaac tggaacgacc tttttttgcc ctgtacaaag
     3541 gcggctgtgc aatataaaga tagccatttt caataatttc aggcatttgc cggtagaaaa
     3601 aagttagtat caaagtcaaa atgtggaggc cgtcaacatc tgcatctgtc atgataatga
     3661 ttttatggta acgtattttt tcaagatcaa actcttcttt cccaatacct gttcccattg
     3721 cagtgatcat ggattttatt tcttcatggg aaagcatctt atcaaaacgg gccttttcca
     3781 cattcaatat tttacctttg agaggcagaa tagcctggtt tttgcggtca cgcccctgct
     3841 ttgccgagcc tcctgcagaa tcaccctcaa ccagaaacaa ttcactcaat gccggatcag
     3901 attcctgaca gtcagccagt tttcctggaa gagtactgaa ctcaagtaca ttttttctgc
     3961 gggtaatttc tcgtgctttt tttgcagcaa gcctggctcg ttgtgcatca atggctttgg
     4021 caacaatctt ttttgcaaca gcaggattct cctccagatg tgcaccaaga tattctgaaa
     4081 ccagggtttc aacttttccc ttaatttcaa cattggtcaa ttttattttt ttctgcgact
     4141 caaactgagg attggtaaca cgggtactta tcactgccgc cattccttcc ctgacatctt
     4201 cgcccgttaa attctcttct aaatcattag taattttatt ggcactcatg tagctgttca
     4261 aagtgcgggt aagagctcct ttaaatcctg tcagatgagt tccgccatcc actgtattta
     4321 tattattaac aaaagaatat attcgctcgg tataactatc gttgtactga aaagcaactt
     4381 cgatctcaac atcttcagat ttcccggaga aataaaccgg agattcatgc agaacatttt
     4441 tattttcgtt gatgtgctct atgaatgctg caattccacc ttcatattta aattcctgaa
     4501 accggtcaga gcggttgtcc ctgattgaaa ttaaaattcc cttattcaaa aaggccaatt
     4561 ccctgagacg atgagctaat atttcaaaat taaaatctac attatcaaaa atagtcttat
     4621 ccggccagaa cgtaatatgg gtcccggttt ttttgctttc ctcaatttgt tcaagggaac
     4681 ttgcagttat tccctgctga tattcctgct tccacagaaa tccttcgcgg tgtatttcag
     4741 caatcatttt accagaaagc gcattcacaa ccgatgcacc aactccattc agtcctccgg
     4801 aaaccttgta gttgctgtta tcgaactttc ctccggaatg aagacgggtc aaaatcagtt
     4861 caactgccgg aattccttcc tcttcgtgta ttccaacagg aattcccctg ccgtcatctt
     4921 ctactgaaag acttccgtcg gaattgatta taacgtgaat ctcagaacaa taacctgcta
     4981 aagcttcatc aacactgtta tctacaactt caaaaaccag atgatgaagt gctgtactat
     5041 ccactcctcc aatatacatt cccggacgca ggcgaacggc ttccaggccc tgcagtacgg
     5101 ttatatcaga ttcgtcgtaa gtcgatttat ctacagtttc aatttctggc attttgttct
     5161 ttcgtgaagc agtcgaagtc tcaaacttca actgtgttgt caaaccaact ctcccttgac
     5221 aatattaaat ttttttactg tcagggatcc agatggttca gaggtagagg taataaaaat
     5281 ttgattctta agtgttaaaa aatattgaaa aacatttttt atcacctcat catccaattc
     5341 tgaaaaaaga tcatcaaaaa tcaaaaccgg ataaaattga tatccctcat agaataaacg
     5401 atttatcgtc atttttaaag acaaattagt aatcctgaac tctccctgcg aaaaaaaatc
     5461 tctgtctttt tttccgtcca tcatcaaatg gtactcatcc ctgtgcggac caagtacagc
     5521 aaagccgtac tgcaggtctc ttgacagact tttttcaagt tgctgaagac aattcttctc
     5581 ttcataatca gctgaattca aagacggttt ataaatcagc ttcaatgttt ctgtgcgacc
     5641 ggaaatatct ttgaaaatat cattcaaatg ttgattaacc tgctctacat aactgtttct
     5701 atgcttcatt aactgaactg cagactttgc cagcatttca ttccatagtt ggatttgttc
     5761 aatttttccc tgtctcaata aagcattttt ttgggaaata atttttgaat attcctgaat
     5821 attattaaaa tagagaggat cattgaaagc cataatcctg ttaaaaaact tgcggcgctc
     5881 ctgtggaaca cttcggaaaa gattaacatc ttccggagtg aaagacagtg ccatgaaaga
     5941 aaaaatatat tcagaaaccc ggcgggaagg tttgtgatct aaaaaaacct gtcgaccttt
     6001 ttttgtgata ttgacacgaa caagatgcag aacttttttt cgttctacct tagcctgaag
     6061 aactgcctct gacttttttt cctgcagcag cagagacggc tttgttgtgc gaaatgattc
     6121 taagttacaa aggtaaaata ctgcctcaac caggttcgtt ttaccctggc cgtttgcacc
     6181 gtaaatccag tttatttccg gagaaagttt tgtcgtacat ctactgtagt tgcgaaattg
     6241 agatacctca atgttgcgga tgatcatgta caatcatttg cggttgacgg ttgactgtcg
     6301 acagtcaaca agtgatcagg aacagtaaaa cgaaaaccgt ccagctacca ttcgactctc
     6361 aatggcataa ttactgacaa aaaggattca tcaacagtgg atttgatgat ggtgggactc
     6421 atcgaatttt tacactctag ctttatatct tcactttcaa tcacaattaa aacatccaac
     6481 agatatctgg agttaaaccc aatttgaaaa ggatcacctt cgtagccgat ttctatttca
     6541 tctaccactt ctccataatc tgctttctca ctttccagtt tcaaaacccc ctgagagata
     6601 agaagtttca ctggtcttag tttttctgaa ctgattgaag agacaattct cagtgaattg
     6661 ataaaacttt cacggttcac aataatttcc attgtgttgt ctttgggaat gataggatcg
     6721 cagtttggaa attttccttc aatcaacctt gtcttgaaac tgatcctgcc gccggtaaac
     6781 tgcataacac gttcatcgaa agaaattacc actgtctgac caaaaagatc tgctgtccgg
     6841 cgcacttcag tcaaagcttt tctgggaacg ataacttcct tatcttcact aaaattcaga
     6901 tcatcgactg atttgagaat ttgtgctaat cgatgaccgt cagtggaaag ccagcgggtt
     6961 tgctggtcac tcgtagaact gagacaaaca cccatcaggt tccgtcgaga ctcgttggta
     7021 atagcagcaa acaatgtcat gtcaatacac tgcttcaaat catccacaga tatcgtcaca
     7081 gattgaggaa ggtcttccag tactgtttgt gggtataagc cgacttcaac cgaaggaaga
     7141 cgcatctcag aagaaccaca ggtaatatga atccacagtt gttctgtaga acgaatcttt
     7201 acatcctcgc cctgaagttc tttgactata tcgaatactt tttttgcatt gacgcagata
     7261 ctgcctggtt ctgtgatttc tgcttccacc tgctcaacaa gagaaagctc ataatcagtc
     7321 gacgaaaact caacctttga gacttctccc tccatggtcc tgataacgaa attagacagg
     7381 attggtttgt ttgaacttgt acttttgtca acaacagaat taatgttttg cagagtacta
     7441 ctgaatgctt ctctttttaa attaatttcc atatttaatt attattagta gtaggtttgt
     7501 tgacaacttg ttggcaagtg aataactttt tgaattataa gaatttttac ctgattttta
     7561 tatgttgaca acttgttggc agaatgttga caaacaataa tttatcaaaa ttaaagaaaa
     7621 aacccaacac gaattgttgg ttagttgttg ataactaatc agtaacaaac atctgaccgc
     7681 aataagttga ttttttacag gtaaaatatt tgattaaatg ttgacaacat gttgataact
     7741 gttagcagtc agttagatgt ggataaattt attaatccac aatagcctga taaaacaagg
     7801 ttttaaatat aaattgatgt cagtagcaga tcatccttta ttgagaacct gccggaatgt
     7861 tgaaaacctg cagacgtaat accggtacat gcttttgaga gtaaaaaaga aaattcggaa
     7921 tttttgtcat caattattat ttctgcatcc tgaaaataaa ggtaggtttg tgagatcggg
     7981 aaaaagcatt tgaagattga ctctttagcc gaaaa
//
LOCUS       DTVD01000145            7873 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2606_length_7873_cov_311.293809, whole genome
            shotgun sequence.
ACCESSION   DTVD01000145 DTVD01000000
VERSION     DTVD01000145.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_7873_cov_311.293809"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="EYO68_05055"
     CDS             complement(<1..845)
                     /locus_tag="EYO68_05055"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HIC07209.1"
                     /translation="MSIQSNYVYTRGYRGKVKGLVLDWSGTTADEYVLAPAVVFVDVF
                     KKYNVEISMAEARGPMGLRKDLHILELTKVPEIRERWKTVYGKYPDQSDVDDMFKDFV
                     PMQLACLRKYTTLLPHVAEVTQKFQKEGIKIGSSTGFVRSMVDILEEDAKKQGYTPDA
                     SVAGDEVINGARPKPFMVYRNLDLMNVHPIQSVVKVDDTVSGVGEALEAGCWGVGIAR
                     YSNYMDMDTLEEAGSLPEEEIQRRLIKTRDILQKAGAHYVIDTFDQLVDVVDDINLRL
                     SRGERP"
     gene            1401..2507
                     /locus_tag="EYO68_05060"
     CDS             1401..2507
                     /locus_tag="EYO68_05060"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HIC07210.1"
                     /translation="MKRNILLNPGPATTTDSVKQALMVPDICPREQEFGDLTQSVLKK
                     VVQVVNGNLTHSAVIFAGSGTAGVEAALSSVVASDGKILILDNGAYGKRAETIIQAYG
                     ISHRTYRIGWGEYPDVAEIESIFQQDRELTHFAFVHHETTTGMLNPLPELLELCQKYE
                     VDSIVDAMSSYAGLPIDLQELPIDYLISSSNKCIQGMAGTSFVIANNEKLAKIAKFPA
                     RNLYLNLWGNHSYIEKTGQFQFTPPVQIVYALNAALDEFFVETQAGRTARYFKAYETL
                     LDGIDKVGLELFLPKSQHSKLLTAIVEPQSPNYDFMEMHDYFYENDITIYPGKGAKQD
                     TFRIANIGAIDYRDMLLFNEKLLQYFEDRKISNS"
     gene            2816..3853
                     /locus_tag="EYO68_05065"
     CDS             2816..3853
                     /locus_tag="EYO68_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC07211.1"
                     /translation="MFNNKKIITLILGSLLWFPHFAPAAQVNILSERQEFLLRPFLDV
                     FEEQTGIKANVVYLKKGSLERLKQQPGSVDALLTVDISNLTAIADAGLFQPINSNVIE
                     QNVPGNYRDPKGLWTALTARARVIYYSKERIKVGELSTYEALGESGNNQRVCTRSGYH
                     KYNVALISSMIAEHGVAKAKTWLQGLKNNRGRKPSGNDRGQVKAIYQGQCDVALGNTY
                     YMGKMLEREDQRAWAASVGIYFPNQGDRGTHMNISGGAVTKAAKNKAEAVKMLEFLSG
                     KLAQYMYAQVNHEYPVKSGIQYSGIVQGFGGGQDGVKKGVFKQDQRNLAEIGSHRKKA
                     IKILDEVDFDN"
     gene            4093..4782
                     /locus_tag="EYO68_05070"
     CDS             4093..4782
                     /locus_tag="EYO68_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIC07212.1"
                     /translation="MTEHSKVIEEYLLTLYKLNRAGEPAKAVTLANRLDTSPPTVHAT
                     ILRMQRDGLVNVTKTKELALTKEGQSIAEDIAYRHNLSEYFLCNTLGIPWYEVHQHAH
                     LLEHAMTPMVVEKLAEFLGKPEFCPHGTPMPGTELPENTITLMEARPGMTVEVAMIGE
                     VLEDSVDLMKILHEHKVMPGKQHKVIEKTEVMRSISLESKEGPSSLPFHVAEKIFVIE
                     IPKDKKRSIQP"
     gene            4802..6448
                     /locus_tag="EYO68_05075"
     CDS             4802..6448
                     /locus_tag="EYO68_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HIC07213.1"
                     /translation="MKSSVHSSGSWNLLTLSLALLFALPFFSLIVTAGSGGENVWGHL
                     LSTVMPGYLKNTFWLMLGVGSGTLLLGVSTGWLISMTEFPGRRMMEWALLLPLAFPGY
                     IIAMVYVELLEFSGPVQEQLRLWFGWQNYMDYWFPPIASLGGAITMLSLVLFPYVYLL
                     ARTAFLQQSATLMEASRMLDKSAGNSFFMIALPLARPAIVVGVSLAMMETLADFGTMQ
                     LFAVQTFTTGIYHTWFGAYNAPGAAQLSLSLLTFISFFIFLERYSRKQQRFHAQSSIK
                     NAHTRYPLSGIRAVLALLFCIFPVLFGFALPAGLLIQWATESWRGVMLDRFFNDAGNS
                     ILLAGVTALLGVFIGLLMAFGSRLSKRRRIHWAARLVSLGYTFPGPVVALGVLLPFAW
                     FDRTMDAWMQETFGITTGYLLSGTLFILVFAYLVRFLALAYGTAESGLERITSDMEDA
                     SRSLGKNTWQTLKQVHLPLLRGSVLAAGMLVFVDVMKELPATLMLQPFNFSTLATRAY
                     GYATEELLKEASLWCLTIVVVGLFPVIFLNRQLRQTAPQN"
     gene            6460..7512
                     /locus_tag="EYO68_05080"
     CDS             6460..7512
                     /locus_tag="EYO68_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07214.1"
                     /translation="MIECRNLTKSYGVLEAVSDFSLHLEEHEFLSILGPSGCGKSTLL
                     RLIAGLEVPSKGQVLLEKREISGEKIILPPERRKFGMIFQDFALFPHLSIEKNIAYGV
                     SGSRSEKQKRVVELLKLVSLQHLASKMPHQISGGEQQRIAVARSLAPRPRLILMDEPF
                     SNLDYQLRLQLRREIRGILKHEGVATILVTHDQVEAITFSERVLLMHKGKLVQAGTPE
                     EIYQQPQTLWASSFVGEANHLSVEWEKTSLKSPFGFLDVPAEIGKETRILMVRPEDFI
                     LVSAPDDEANGIVKTVEFSGAAQTVGVELKSGEKIQVSGSPHLLWNLEDPVKITAERY
                     LCFNSSGDRLDGFTAK"
     gene            complement(7650..7712)
                     /locus_tag="EYO68_05085"
                     /pseudo
     CDS             complement(7650..7712)
                     /locus_tag="EYO68_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT         2234 a         1685 c         1707 g         2247 t
ORIGIN      
        1 ggacgctcac ctctggagag gcgcaggtta atgtcatcaa caacatccac caattggtca
       61 aaagtatcaa taacatagtg tgcaccagct ttttgcagga tatccctcgt tttaatcagc
      121 ctgcgctgga tttcttcttc aggaagcgaa ccagcctctt cgagtgtatc catgtccatg
      181 tagttgctgt aacgagcgat acctactccc caacagccgg cttcaagcgc ttcaccgaca
      241 cctgaaacgg tatcgtcaac cttaaccacc gactgaattg ggtgaacatt catcagatcg
      301 agatttcgat aaaccataaa cggcttgggt ctggcaccat taatcacttc gtctcctgcc
      361 acggatgcat ctggagtgta cccctgtttt ttcgcatcct cttcaaggat gtctaccatg
      421 ctccttacaa aacctgtaga actcccaatt ttgatacctt ctttttgaaa tttttgagtc
      481 acctctgcga cgtggggaag gagggtggta tacttcctga gacaggcgag ctgcattggg
      541 acaaaatcct taaacatatc atccacatca ctctgatccg gatacttacc gtataccgtt
      601 ttccagcgct cacgaatttc cggaaccttg gtcaactcaa ggatgtgaag gtccttgcgc
      661 agccccatcg gccctcttgc ttccgccatg ctgatttcaa cattatattt cttaaaaaca
      721 tctacaaaca ccacggccgg ggcaagtacg tattcatctg cagtggtgcc gctccagtcc
      781 agtaccagcc ctttgacttt tccgcggtag ccacgggtgt aaacgtaatt tgattgaatg
      841 ctcattttga tctccttttt gtgagtgaaa aaatattttc cggaaactcc ggatacagta
      901 gaaaatattt attttaacaa caatatcaat atctgtataa tatcaatatt gtatcttatc
      961 tattgattta aacaatagta aattttttcc ttcaacaata gtaagcttcc tgaaaaataa
     1021 tattttccaa taatatcatt aggctataat tagccccatg aaatcaactg caagtggttt
     1081 tcctgaaaaa tgacaaaagc cctgtagttc tgttgtttga gtttatttta caaggagatt
     1141 aatcacgagt ataaaggttg ttactcttca attcatggac tcaacctttt tgggaatctg
     1201 gatcccggat taagtccggg atgacaagaa aataatattt tagtaagccc tgtttatctt
     1261 aaaatatttt actactattg atttaatcaa tcgaaagaat tgtttatttg cattttacag
     1321 atacgtaaag atttagtaat atttaacatt attgaagaag aattcaccat aatttctcaa
     1381 cagtccaagc taattatctc atgaaacgta atatccttct caatccaggt ccagccacaa
     1441 cgactgacag cgtcaagcag gcactgatgg ttccggacat ttgtccccgc gaacaggaat
     1501 ttggtgacct cacccagtcc gttttaaaaa aagtggttca ggtggttaat ggcaatttga
     1561 cgcatagcgc tgtcattttt gccggatccg gaactgccgg agttgaagca gccttgagtt
     1621 cggttgtagc ttcggacggc aaaattctga ttctcgataa tggcgcttat ggcaagcgcg
     1681 cagaaacgat tattcaagcc tatggcattt cgcaccggac atacagaatc ggttgggggg
     1741 aatatccgga cgtggcggaa attgaatcaa tatttcagca ggaccgtgaa ctgacgcact
     1801 tcgcttttgt gcatcacgaa acaactactg gaatgctcaa ccctctgccg gaactattgg
     1861 aactttgcca aaaatatgaa gttgattcaa ttgttgatgc catgtcctct tatgcaggac
     1921 tgcccattga cttgcaggaa ctgccgattg attatttaat atcttcttca aacaagtgta
     1981 ttcaaggcat ggcaggtaca agttttgtga ttgcaaataa tgaaaaactt gcaaaaattg
     2041 caaaattccc ggcgcgtaat ctttacctca atttgtgggg aaatcacagt tacattgaaa
     2101 aaaccggaca atttcaattc acgcctcctg tgcagattgt ttatgctctt aatgcagctc
     2161 tggatgagtt ttttgtagag acacaggctg gacggactgc acgctatttc aaagcttatg
     2221 agaccctgct ggacggtatt gataaagtcg ggctggaact ctttctgccc aaatctcaac
     2281 actccaagct gttgacggca atcgtagaac cgcaatcccc aaactacgat tttatggaaa
     2341 tgcatgacta tttttatgaa aacgatatca caatctatcc tggaaaagga gccaagcaag
     2401 atactttcag gattgccaac atcggtgcaa ttgattatcg tgacatgctg cttttcaacg
     2461 aaaaacttct tcagtatttt gaggacagga aaatctctaa ttcataatat attaataccg
     2521 acacttttct gaatcgattg cgaaaattct tactggttgc tatcgtgaaa taaaaaatta
     2581 ggctttttaa ttaaattagt gtagactaat aattttattt tgcttacaat aactaccatg
     2641 ctggtatttt tgtcagggca atccctacta caactcctcc ttctctttca tcgaagttgc
     2701 agtatttcgg ggaaaatcga taaaggctca agctggaaga tctcttacaa agaattaaat
     2761 tttttatcaa catcctgact caggtttatt tttaatattt ttgtaaagaa atactatgtt
     2821 taacaataaa aaaatcataa ccctcattct cggaagtctg ttgtggtttc cacattttgc
     2881 cccggctgca caagtaaata ttctttcgga gcggcaggaa tttttgctcc ggccttttct
     2941 ggatgtattt gaagaacaaa ccggaatcaa ggccaatgtt gtttatctca aaaaaggctc
     3001 tctggagcgc ctcaagcagc agccgggttc ggtcgatgcc ctgctgacag tggacatttc
     3061 aaatttgacc gcaattgcag atgcaggact atttcagcca ataaactcaa atgtaattga
     3121 acagaatgtt ccgggaaact accgtgaccc aaaagggctt tggacggctc taactgctcg
     3181 cgcccgtgtg atttattatt caaaagagcg tatcaaagtc ggtgaattat ccacctatga
     3241 ggcattgggt gaatccggaa acaaccaacg tgtctgtacc cgttccggat accacaaata
     3301 caatgtggca ttaatttctt caatgattgc agaacatggt gtggccaaag cgaaaacttg
     3361 gctgcagggt ttgaaaaaca atcggggacg caaacctagc ggaaacgatc gaggtcaggt
     3421 caaagccatt tatcagggac agtgtgatgt cgcgctggga aacacatatt acatgggtaa
     3481 aatgctggag cgtgaagacc agcgtgcatg ggccgcatca gtaggaattt attttccaaa
     3541 tcaaggtgat cgtggtaccc acatgaatat ctccggaggc gcagtcacta aagcagcaaa
     3601 aaacaaagct gaggctgtaa aaatgctgga gtttctttca gggaaactag cccaatacat
     3661 gtatgcacag gtgaaccatg aatatccggt caaatccgga attcagtatt caggaatcgt
     3721 acagggtttc ggtggtggac aggacggagt caagaaaggt gttttcaaac aggatcagcg
     3781 taatttagct gaaatcggca gtcaccgaaa aaaagccatc aaaatacttg atgaagttga
     3841 ttttgacaat taagcacaaa gatttcagga acattaattt ttaatcagga actcgctaca
     3901 tgacagttga aaaaatttca catgtaattt gttattgcga gcctaactga gtttctgcag
     3961 ctgaaatggt ttctgccgtt ttcagttgta cattaacgaa aggccgccgc tcggctaccg
     4021 gaagatagag gttgtcattc tcaaacattg ctgttagtat atgctaattt caaaccccaa
     4081 ttaaaaagtg ctatgactga gcattctaaa gttattgaag agtatctgct gacactctac
     4141 aaactgaacc gtgcaggaga gccggcaaaa gcggttacac ttgcaaaccg tttggacaca
     4201 agtcctccaa ccgttcatgc aacaatctta agaatgcagc gggatggatt ggtcaatgta
     4261 acaaaaacaa aagaacttgc attgacaaaa gaaggccaaa gtattgctga agacattgcc
     4321 taccgtcata acctctctga atattttctt tgcaatacac tgggaattcc atggtacgaa
     4381 gtgcatcagc acgcacacct tttggaacac gcaatgactc ctatggtagt tgaaaaactt
     4441 gcagagttcc tcggtaaacc cgaattttgc ccacacggta caccaatgcc cggaaccgaa
     4501 ttacctgaaa atacaatcac tctcatggaa gcacggccgg gaatgactgt tgaggttgcc
     4561 atgattggag aggtattaga ggattcagtt gatttgatga agatcctgca tgagcacaaa
     4621 gtgatgcccg gcaagcaaca caaagtaatt gaaaaaacgg aagtgatgcg ctccatctca
     4681 cttgaatcca aagagggtcc ttcgtctctg ccttttcatg ttgcagagaa aatctttgtg
     4741 atagaaattc ccaaagacaa aaaacgttcc attcaacctt gaattcatct cccagaaaaa
     4801 aatgaaatct tccgtccact cttccggaag ctggaatctt ttaacactct cgctggcttt
     4861 gttgtttgcg ttaccttttt tctctctgat cgtaactgcc ggttcaggag gagaaaatgt
     4921 atggggacat ttattaagca cggttatgcc cggttatcta aaaaacacat tctggctgat
     4981 gcttggtgtt ggcagcggca ctcttctttt aggagtgagt acggggtggt tgatttcaat
     5041 gacagaattt cccgggcgtc ggatgatgga atgggcattg ttactgccgt tggcatttcc
     5101 cggttacatt atcgcgatgg tctacgtgga actgctggaa ttttccggac cggtgcagga
     5161 acaactccgt ttgtggttcg gctggcagaa ttacatggac tactggtttc cgccgattgc
     5221 ctcacttgga ggcgcaataa cgatgctctc gctagtactc tttccttacg tttatctgct
     5281 tgccagaacc gcgtttcttc aacaatcggc tacattgatg gaagccagcc ggatgctgga
     5341 caaaagtgca ggtaacagtt tttttatgat tgccttgcca ttggcgcgtc cggcgattgt
     5401 ggttggtgtc agcctggcaa tgatggaaac cctggcagat tttggcacaa tgcagttatt
     5461 tgccgtacaa acctttacta ccgggattta tcatacatgg ttcggagcat acaatgctcc
     5521 gggtgcagct cagctttctt tatcgttact gacgttcatc agcttcttta tatttttgga
     5581 acgctactcg cgaaaacaac agcgtttcca cgcacaaagc agtataaaaa atgcacatac
     5641 cagatatcct ctttctggaa tccgtgccgt cctggctttg ttattttgta tatttccggt
     5701 actgttcgga tttgctttgc ctgcaggttt attgattcag tgggcaactg aaagctggcg
     5761 tggagtgatg ttggaccgct ttttcaacga tgccggaaac agtatcttac tggccggcgt
     5821 aaccgcatta ttaggagtat ttatcggcct tttaatggct ttcggttccc gactcagcaa
     5881 gcgccgcaga attcattggg cagcacgcct ggtaagtctc ggttatactt tcccgggacc
     5941 ggttgttgcg ctcggtgtcc tcttgccttt tgcctggttt gaccggacaa tggatgcatg
     6001 gatgcaggaa acatttggaa taactactgg ttatctgctg agcggaactt tgttcatttt
     6061 ggtttttgct taccttgtac ggtttcttgc cctggcttac ggtaccgccg aatcaggact
     6121 tgagaggatc acttccgata tggaagacgc aagtagatct ttgggaaaga acacctggca
     6181 aactttaaag caggtacatt tgccgctgct tcgaggcagc gtgttagccg ccggaatgct
     6241 ggtctttgtc gatgtgatga aagagttgcc cgcgacactt atgctccagc cttttaattt
     6301 tagtacactt gcaaccaggg cttacggtta tgccacagaa gaactgctca aggaagcctc
     6361 tctctggtgc ctaactattg tcgtagtcgg tttgtttcca gtaattttcc tgaatcgcca
     6421 actgcggcaa actgcaccgc aaaactaact tatttaacca tgattgaatg tagaaatctc
     6481 accaaatcct acggcgtact ggaagccgtc agtgacttca gcctgcattt ggaggaacac
     6541 gaattccttt ccattcttgg accatccgga tgtggaaaat ctactctgct gcggctgata
     6601 gcaggactgg aagttcccag caagggacaa gtattgctgg aaaaacgaga gatctcaggg
     6661 gaaaaaataa tcctgcctcc ggagcgtaga aaatttggta tgatttttca ggattttgcc
     6721 ttgtttccgc atttgagtat agaaaaaaac attgcttatg gtgtaagcgg atcccgaagt
     6781 gagaaacaga aacgggtggt ggaattgctg aagttggtca gtttacagca ccttgcatcc
     6841 aaaatgcccc atcaaatcag cggtggagaa cagcagcgaa tcgccgttgc cagatcactt
     6901 gccccacggc caaggctgat tttaatggat gaaccgttca gtaatttaga ttatcaattg
     6961 cgtttgcagc taaggcgcga aattagggga attcttaaac atgaaggcgt ggcaacaatt
     7021 ctcgtaacac atgatcaggt tgaagcaata actttttctg agcgtgtgct cctcatgcac
     7081 aaaggtaagc tggtacaggc tggaactccg gaagaaatat accagcaacc gcaaacactt
     7141 tgggcctcat cttttgtggg tgaagccaac catctttcgg ttgaatggga gaaaacatca
     7201 ttaaaatcac cttttggttt tttggacgta cctgctgaaa tcggtaaaga aacccgcatc
     7261 ctgatggtac gtccggaaga ttttattctt gtgtctgctc ctgacgacga ggcaaacggc
     7321 attgtgaaaa cggttgaatt ttctggtgca gcgcagacag tcggagtgga attaaaaagt
     7381 ggcgaaaaaa ttcaggtaag cggttctcca catttattgt ggaatctgga ggatcctgtg
     7441 aaaatcacag cagaaagata tttgtgcttc aacagttccg gtgacagact tgacggattt
     7501 actgcaaaat aaaagtgcaa tagcttttaa cacactagaa actttcaaat gtcttgttct
     7561 gggaaaagca tctgatcata tctggcgtct tatcaatgtg aagtcacaat ttatgttatt
     7621 aagtcttcag tttcctcttt ggatcattaa cacataaagt tgccagggaa tctttcaata
     7681 tttcttctta ctacttgttt tagcactttc gtaccaaaac tttacaactg atatccaact
     7741 atcagctgaa tggttttgaa ttgtgaagtc tatacaggtg tggtgaaatg gtgaggatta
     7801 attaaagact ttaatcgaaa tagcgaacca tcatcaaaag actcacgtgc caacagaaac
     7861 aactaaaata act
//
LOCUS       DTVD01000146           26127 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_262_length_26127_cov_307.606935, whole genome
            shotgun sequence.
ACCESSION   DTVD01000146 DTVD01000000
VERSION     DTVD01000146.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 26127)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26127)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26127
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_26127_cov_307.606935"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="EYO68_05090"
     CDS             complement(<1..1136)
                     /locus_tag="EYO68_05090"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC07215.1"
                     /translation="MKQNNTADIIIIGGGIIGCSIAYNLANQGAKNVVVLEKGELCSG
                     GTAKSCAITRSHYSIEANVHHAVESVKIFENFDDMVGGDPRFTCTGQLVLGQEKHRPV
                     MESVFCTQNKYGSETQTLTPAEAAKLHPLLQFEDVDVIGFESRSGYCDPYLTTIAYAK
                     RAKDLGVKFYTGTPVTGIRINGVNKTIETRRSDFECSTLILAAGPWTHNLGKMLGIKF
                     PYEISKHKVLTLKIDIPYEKDWPIVKDLLTPEKIYFRPDAGGVVLVGTGDQGDPVNNV
                     EEMTNDVEMEHIERIGKLMANRMPAFADAKLVRSWTGPYDIPPDWNPIIGPVPKLEGV
                     HVAVGFSGHGFKLAPTIGESLAQQILGTNPRVPIDMYDMTRFETG"
     gene            complement(1170..1925)
                     /locus_tag="EYO68_05095"
     CDS             complement(1170..1925)
                     /locus_tag="EYO68_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIC07216.1"
                     /translation="MTTNNWTPEQPIVSLNNVYKSFGELEVLKGISFDVMKGEVICII
                     GPSGSGKSTLIRCINALNDINSGSIKVHGLEVNDSNLNKLELRKNVGMVFQQYNLFPH
                     KTALENIMMAPELVLKEAKEDVLVKAQALIRKVRLEDKQNNYPAELSGGQQQRVAIAR
                     SLAMNPDVMLFDEVTAALDPETVKEVLVTIKELAAEGMTCILVTHEMGFARELADHIY
                     FTDRGIIVEHGPPATFFKKAKDPRTKEFLSQIL"
     gene            complement(1935..2840)
                     /locus_tag="EYO68_05100"
     CDS             complement(1935..2840)
                     /locus_tag="EYO68_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIC07217.1"
                     /translation="MFGYLNHIPGISPNSQQSDLPVLLPYQGHSAGKPEDLRFSRWLA
                     SSSPWVLLVIFLLISFYPLAVLAQSKYTLIQAFATLWRWLPFVVGSGFVMNLLISFFT
                     MLFGTVAGVFLGLGQISVVAPVRKFSWFFTQLFRNSPWLVLLFIVMLTFPFEIEIGEH
                     IISIPDWMKAVFGLSLPIMANISEVVRGAVQSVPTGQWEASESLAFSRVQMIWKIILP
                     QCFKRMIPPWMNWYAILTMATPLVSLLGVQEIVTLSRQAMEAEDNHPELLIPFFGFAL
                     LIFFAYCYPIARWTLKLEERYSVKL"
     gene            complement(2890..5337)
                     /locus_tag="EYO68_05105"
     CDS             complement(2890..5337)
                     /locus_tag="EYO68_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC07218.1"
                     /translation="MSTKLPKQSQIVIIGGGIMGCSTAYHLLKNGCRDVILLERTKLT
                     SGTTWHSAAQVRQLRSTESLTRLVQNSVALYSSLEAETGQATGWKQTGSLSIATNPDR
                     LTHIRRQASLSQAFGIGAEEISVSNAAEKWPLMRSDDLIGAVYSPSDGRVSPSDICAA
                     LIKGAKSQGLKVFEDTPVTGIRTENGRVATVQTAQGEISCETVVNCAGIWGRSIATMV
                     GAVAPLHACEHFYLLTELMDSVTAPLPTLSDHDGHLYLRDEGGGLLIGCFEPQGKALD
                     IETLPEDFAFDLLPEDWDHIEPILANAMHRIPELEQTGVKMLLNGPESFTPDDRFLLG
                     ESPELRGFFLGCGMCSVGIATGGGAGRALAEWIIDGEPSMDLWPVDIRRFVPAQNTLR
                     TLRERSPETLALHYAVSFPGRQHQTARNLRLSPLHSRLENAGAEFAERMGWERPRWFN
                     PENKPTAPELSFEKPGWHSLHAEEHRAAREAVVLFDQSTFGKLLVQGRDAESVLQRLC
                     ANDISKADRRVVYTAMLNKHGGYESDLTLMRLDADSFLLVTGTGQPVRDKDWIRRNLN
                     PDEFVTVTDVTGAYAVISIAGPNSRKLLSRVSLDDFSNYGFPYYTHRTIEVGPAMVRA
                     ARLSYVGELGWELYIPSESALPVFDALSTVGDDLGLKLAGVEALSSLRIEKGYCAWGH
                     EIGPDDTPLQAGLEFAVKFNKPESFIGKEALLKQKTEGDTRHLVMLTVADSEAFPHGG
                     EPIWWNNKLVGRTTSGTFGHTLGCAVMMGYIGWVETKLDTMLNTGNFEVEIARRHFPA
                     QVSLDAPYDPKGLASRK"
     gene            complement(5403..6158)
                     /locus_tag="EYO68_05110"
     CDS             complement(5403..6158)
                     /locus_tag="EYO68_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIC07219.1"
                     /translation="MPEAISHFFLELYKSDPKWNFIFFYDPVQMDRVIEGFWTTIELA
                     VVCVLISVVIGIVGAWMQNQHNRFLRLLVQGYIQFFRNTPPLIQLMFFYFALGQFTPT
                     YSPDGWLEIPIISNVGWAIISLSFFAGAFNVEIFRAGIEAVPESTKEASEALGFSHLQ
                     TYRYIVLPLAFRVSLPSLNNNLVNLVKTTTQAMAIAVPELLYQMVSIYNDYSTAMNAS
                     MLLLFVVYIGLVAILVLGMSRWEENSRIPGYGG"
     assembly_gap    6199..6298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6418..7269)
                     /locus_tag="EYO68_05115"
     CDS             complement(6418..7269)
                     /locus_tag="EYO68_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIC07220.1"
                     /translation="MKVLNKMILGTLSATLALSLVSTAASAGQCTNERWNKVMKRGKI
                     VIGVKADYKPWGYRNTAGGLVGMEIDMAKDVADAMGVKLELVPVQSSNRMQFLEQGKT
                     DMMIATMSDRVDRRKIVGITQPNYYTSGTNIMSPKALGLTSWEDLRGKPVCGKQGAFY
                     NKIVADRYGAKIIAFTGNAEAKQALRDKKCIAWVYDDSSIMSDLSSGNWDDFEMPLAS
                     EDDNPWGLAVPLEELSCVFGNFMTGMTYNWHQSGRLIELEKKHGIQATNYLVIQKFRS
                     KDWLEGK"
     gene            complement(7520..7713)
                     /locus_tag="EYO68_05120"
                     /pseudo
     CDS             complement(7520..7713)
                     /locus_tag="EYO68_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280526.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(7846..8640)
                     /gene="hutG"
                     /locus_tag="EYO68_05125"
     CDS             complement(7846..8640)
                     /gene="hutG"
                     /locus_tag="EYO68_05125"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HIC07221.1"
                     /translation="MNPVEVFEGESPVILGQPHGGTFIPAKVASQFNANGLKIADTDW
                     HIHRLYKGLLPQATVVQATFNRYLIDVNRDPSGKSLYPGLVTTELCPTLDFEGQDIYN
                     KGAEPDAQEIESRLQTYHTAYHAALLEQLNRIKKKYGIVLLFDCHSIRSYLPNLFEGA
                     LPELNLGTNNGKSCDSKIEKVAIDMCEKDGRYNYVLNGRFNGGWTTRTYANPEYGIHC
                     IQLEIAQRTYMDEFVPWKFCEDRAEKLQLYLKNLLRELEIKIKKCL"
     gene            8828..10198
                     /locus_tag="EYO68_05130"
     CDS             8828..10198
                     /locus_tag="EYO68_05130"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIC07222.1"
                     /translation="MKYFAELALIPEGWSKNVRVTIDSSGKIESVESGIEPNHEDIHL
                     KNRILLPALSNLHSHSFQRALAGLTEKRLKNNDSFWSWRVLMYHFLEQLSPEQLEAIA
                     SQVFMEMLECGYTSVGEFHYVHHQEAGKHYDNIAETSCRIITAAHETGIGLTHLPVFY
                     MQSNLDGSKLSQNQLRFGNNSVQYFRILELARAALSNAPKDYLLGIAPHSLRAVSPEA
                     LVEIIEAKTSGPVHIHIAEQLKEVVDFQLKFNARPVEWLLDNTAVDSKWCFIHATHLT
                     SKEIHNSAKSGAVAGLCPITEANLGDGIFDGSGWLLSGGKYGIGSDSNVQISLTEELR
                     LLEYSQRLLKKERNVMSQVPGSVGHALYNDSLVGGAQALGRNSGSITSGNWADLLTLD
                     AESLTFCGCTEDQILDRWIFCGNDSLVREVWSAGRQMVVNGRHIKHSIIEKQYRNVIQ
                     EIQKEM"
     gene            complement(10473..10946)
                     /locus_tag="EYO68_05135"
     CDS             complement(10473..10946)
                     /locus_tag="EYO68_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIC07223.1"
                     /translation="MLHLARYSSITPSDFDKALGITVGFDSMFDRLFGDVERSENQGG
                     FPPYNIRKDEENMYTIEMAVAGFSKEDLEAELKEGVLTVRSKTDQEEGEYLHRGIAKR
                     AFTRSFTISDDVIVKGADLVNGMLTISLERIVPEEKKSRMIEIGQPTESDLKKLN"
     gene            complement(11108..13732)
                     /gene="clpB"
                     /locus_tag="EYO68_05140"
     CDS             complement(11108..13732)
                     /gene="clpB"
                     /locus_tag="EYO68_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIC07224.1"
                     /translation="MDTQKFTEKSLSALENAHSDAVRRKNSELSTVHLLSALTFQENG
                     LVPRIFEKMELDSEKFTQALESSLKSLPTLSGSSAGRVGASGEMNQALVRAEDEAKKL
                     QDEYVSVEHLLLALTEEGRKGPAGKILAENGVTRERLMGTIQDVRGGQRVTSQNPEET
                     YEALEKYGTNLSQRARDGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAI
                     AEGLAQRIVRNDVPDSLKNKKLVSLDMGALIAGAKYRGEFEERLKAVLKEVVDSDGQI
                     MLFIDELHTVVGAGKTEGSMDAGNLLKPMLARGELHCIGATTLDEYRKHIEKDPALER
                     RFQQVLVDQPTVEDTISILRGLKERYEIHHGVRIKDAALIAAATLSHRYISDRFLPDK
                     AIDLMDEAAARLRVEINSMPAEMDEISRRILQLQIEKEALKKENDTASKERLKKLSKE
                     LAELQHSFDNLTLQWEKEKSALQDMSGLKQEIEEVRLQIERARQDYNLEEAARLEYGE
                     LPELEKRLKTTQSEEQSSKNQLLKEEVDAEDIAEIVGHWTGIPVQKLIEGEGEKLLRL
                     PEQLHQRVVGQDEAIDLVSDAVMRARSGINDPNKPLGSFIFLGPTGVGKTELARALAE
                     NLFDDEQNMIRIDMSEYMEKHAVSRLIGAPPGYVGFDDGGQLTEAVRRKPYSVLLFDE
                     IEKAHFDVFNVLLQILDDGRLTDSHGRTVDFKNTLVIMTSNIGSQKLINQNEDGISGS
                     SLLAEQKKLVLQELRQHFRPEFLNRVDDTVVFHPLLQEHMNGIIKIQLERLKKRLEER
                     NISLQLEDKTIDYLAEVGYDPVYGARPLKRAIQKELETALARAILSGEIHDGQEVTAN
                     VSKAKIFFENITSVSNEE"
     gene            14350..14925
                     /locus_tag="EYO68_05145"
     CDS             14350..14925
                     /locus_tag="EYO68_05145"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013795705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIC07225.1"
                     /translation="MAKLYFYYSAMNAGKTTTLLQSAYNYRERGMKTLVLKPKVDDRY
                     NPDRVRSRIGLESDAASFESEDDLLALTEKIDKKTPLSCILLDEAQFLTKTQVYALGE
                     IVDKLGIPVLAYGLRTDFKGELFEGSLHLLAWADELVEIKTICHCGKKANMVLRLDEN
                     GEPLKSGEQIQIGGNDSYVSVCRKHYKSAGT"
     gene            15040..16053
                     /locus_tag="EYO68_05150"
     CDS             15040..16053
                     /locus_tag="EYO68_05150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIC07226.1"
                     /translation="MNLKNVAIAGATGAVGTEFLKLLELRKFPINNLKLLASERSVGK
                     KLEFCGEQITVEELTTKSFQGVDLVFFSAGKGRSKEFVPHAVDAGTVVIDNSSAFRLH
                     PDVPLVVPEINPQMVFKHKGIIANPNCSTIQMVVALNPLHQAATIKRVVVSTYQSTSG
                     AGSKGMRELISQTKAWLNDDPLEVSAFPTQIAFNLFPHVDVFLENGYTREEMKMVNET
                     HKIMDAPDIGISATCVRVPVLRAHSEAVWIETEQKLSPEEAREILKKAPGIIVKDEPV
                     DGGYPTPWEASETFETFVGRIREDFSHPKGLTFWVVADQLYKGAALNSIQIAEVLATG
                     QVT"
     assembly_gap    16322..16421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16431..16853
                     /locus_tag="EYO68_05155"
     CDS             16431..16853
                     /locus_tag="EYO68_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07227.1"
                     /translation="MSKKVVPIPNLLNLKTNEYEFDFRNGNSIQLEFSVQIQNSVSSK
                     IVKRKGLVIFESDYNRIRLPKRFMLDANGYARVLVGIDPPRIDDSKFRGLITIRAFMD
                     EDGFDDVAEGNLVLKIRNSGKIRTLILDPDKGLTETEG"
     gene            complement(16863..18338)
                     /locus_tag="EYO68_05160"
     CDS             complement(16863..18338)
                     /locus_tag="EYO68_05160"
                     /inference="COORDINATES: protein motif:HMM:PF01555.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07228.1"
                     /translation="MQDTEKKSIKDKARSSAGTIPITAADLDKKPIRVRKKSKAAPEE
                     NAGDSKYKRPKVSKPESNYKQNSKLKTQNSAQLPWKRKDWQQTESENTFGAPPKNEKK
                     IKQRRSASQMFLNSESFGEQEGLMLEALGASVKGRSEYDVTQGFHPYPGRFHPDLPKI
                     LLKQFPEGSTVFDPFMGGGTVLLEGLLWKHKVLGNDLNPIAKMVTRERCRWISEKNAS
                     RVWSALEDVRERVNIRSLAKKSVHRRNLSWLSHFHPPYLFVELLHWIDGIENLYSANE
                     RETLVAVFSSLIVKFSKKIFETSENTKQPSFPKGAVGKWMERKTQELLKNQLKLATKI
                     PNTAPAIIWNENILELEGPEENSVNCTITSPPFPGTYDYLKLHELRMKWLDLSSNTMS
                     SSEITNRNYSPGQWKQVFREFMLKLRRWTAEDGLCYLVLGDWVERNKRVSGLEFTQKY
                     ADSIGWKVTGGASVQREIYDSELRKSFGKTGKWEHLILLRK"
     gene            complement(18361..19287)
                     /locus_tag="EYO68_05165"
     CDS             complement(18361..19287)
                     /locus_tag="EYO68_05165"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIC07229.1"
                     /translation="MSDFTSAKTSHRHGVISVLVALPFVSLTGLFGKFLTFSPLLIVQ
                     GRTIFAFGALLLALFLLRKKIFFSEHREWLWLALSGSILGVHWIAFFQAIQVSTVAIG
                     LLSFASYPLFTTLLEPLFFREPLRVRNVFAALIVICGLALMATSSSDDSNTIISGSVF
                     HGILWGLMAGFGFAVLTLLNRGHVRKHSALLITCWQNGFAALVILPWTLSESWILSGK
                     ELGLLFLLGVVCTVGGHTLLINGLRHVRAQVASLLIAGLEPVFAILFALFLLEEIPSL
                     QTLLGGVLIISTTVFIIYGTEQKEIPEQAVSK"
     gene            complement(19297..21189)
                     /gene="mnmG"
                     /locus_tag="EYO68_05170"
     CDS             complement(19297..21189)
                     /gene="mnmG"
                     /locus_tag="EYO68_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIC07230.1"
                     /translation="MKTNPKKYDVLVIGGGHAGCEAALAAARMGADTLLVTLSRDTIA
                     QMSCNPAIGGIGKGHLVKEIDALGGEMGKVADATGIQFRVLNTSRGAATHGSRCQSDY
                     LAYKKFMRHILEKQSGLTIIEAAAEELLLNGNQVVGLRTEKEELYAETIIITTGTFLN
                     GLIHRGSERVDAGRVAEPSAKRLSLSLADQSLKLGRMKTGTPARLDKRTIDWSQLGIQ
                     PGDEKPKKFSFWDSEILLPQVPCHIAYTNERTHLIIQKNLSRSALYGGEITGIGPRYC
                     PSIEDKIVKFADKKRHQVFMEPMGYAEDSLMIYPNGLSTSLPTEVQFEFLRSIKGLEE
                     VEIFEPGYAIEYDMLDPTQLKATLELKTVKNLYLAGQINGTTGYEEAAAQGLMAGINA
                     VLKVRKKQPFILQRNEAYIGVMINDLITRGVIEPYRMFTSRAEYRLHLREDNADARLS
                     QKGWDLGLLPEKYFQHFQQKQQEISRLSGIVKAERITPNKAVQTELKKLGEAPLKTAA
                     KVSDLLKRPKINIDRLLGSESISGSIDWNYYSPVVREQVEINIKYAGYLARQDQELKY
                     VNQLDRIEIPDNLHLSGIPGLSREVVEILDKLKPGTLGQASRITGMTPAAITILRVYL
                     RTKQAA"
     gene            complement(21327..21968)
                     /locus_tag="EYO68_05175"
     CDS             complement(21327..21968)
                     /locus_tag="EYO68_05175"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIC07231.1"
                     /translation="MHIILLGLPGCGKGTQAQNLIRDYGLVQLSTGDMLRAAVKSGSE
                     VGKQAKTVMDSGGLVSDEIVIGIVREQITHADCTKGYLLDGFPRNLSQAKKLDEMLAA
                     QNEQIDNAIELCVDDKTVVTRIAGRRFHLASGRSYHIEFNPPKIEGKDDVTGERLVQR
                     DDDNEETVQSRLITYHEQTKPLVKYYEEKGILVSIDGIGTPDEIFSRIKVVLE"
     gene            complement(22151..22861)
                     /locus_tag="EYO68_05180"
     CDS             complement(22151..22861)
                     /locus_tag="EYO68_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015222481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC07232.1"
                     /translation="MENSAKKVIMISGANRGIGRAITEKLLSENYRMSLGVRNPEQLP
                     ETLASVAETVLLCQEYDARNHDSAKTWIDATVKQFGRIDGLVNNAGILHATGLEDDEE
                     DLLDDMWEVNAKAPLRLTRLAFPYLRKSGSGRIIDIISMSGKRVKGNLVGYSMSKFAA
                     MAASHAARLQGWDDGIRVTAICPGFVRTDMTAEVKTFAQEKMSRPEDIAQIVNTLLLL
                     PNESVVPEVMMNCVFETL"
     gene            complement(22873..24126)
                     /locus_tag="EYO68_05185"
     CDS             complement(22873..24126)
                     /locus_tag="EYO68_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07233.1"
                     /translation="MSSSSFFCGRQKINFFFRLILQVLLWSNILVPKVFSAEFSFSTK
                     SLIATQNSTQYSTSFFFNRFRAQTDFALSENCATEIEYELFPAWIDSAAQASTISIKN
                     SFSRIYRIADLNHSLTGSPAATDTSELWQLEQNLDRALMRCQSGNWEYFIGRQAVTFG
                     GMSVSPTDVFSPVAFKPLDQEFRPGVDAFRIVGGLGETTEFEAGILAGKDLSSENNGA
                     YLSSHFLLGTTDLKPLLASFQQNQLLGLTLQTDLGMLGLVFDGALILQRKSAPETTEE
                     VRNEWAPWTIGLNFQWNENLFTMLDFHHNPMGAKNPGNYVSNSSSTIYSEFPVSLLGR
                     DYLLPNLSYQFSPLLSFSSSAFFNLNDSSFLNTSGLEWNFLEDLLLSWNISLASGIKS
                     SMQTEKNSEFGDTPNSFKLILKHYR"
     gene            complement(24113..24802)
                     /locus_tag="EYO68_05190"
     CDS             complement(24113..24802)
                     /locus_tag="EYO68_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07234.1"
                     /translation="MEETVIVAHKIFKAFGEGETEIQALRNVDLDIKRGDFAAIVGPS
                     GSGKTTLLNILCGLDNVSSGEIKVSGRSLQEMSGSELAQFRREHIGLVFQAYNLIPVL
                     TVLENIEYVMLLQHVPAAERKERVHTFLAALGLEGKGNRFPAELSGGQQQRVAIARAM
                     VSKPDIILADEPTANLDSETGGQLLEMMRKFNEELKMTFVFSTHDPQIMERAKRLITL
                     TDGRIVSDELK"
     gene            complement(24817..26040)
                     /locus_tag="EYO68_05195"
     CDS             complement(24817..26040)
                     /locus_tag="EYO68_05195"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07235.1"
                     /translation="MLLKLAWRNLWRNRTRTWLSALVIAIGLVAMIFMDTMMVGMNTN
                     MVKNATDSLMGHAQMHALGFRDEQDVKLTINDLDQALLQLEKHPDLKARSLRIVTQSM
                     LSSSGGGEPVFSLGVDPEEEQLLSKFDEAIIEGEYLDSVSGQKLILGWKLAEKLELGL
                     GDRLVLTAADAESGEMVQELFRLSGIFKTGEEKMDASMVLVARKTLQSMLKLEGRIHQ
                     IALRYSKLTQDGTPVNPLKKPDDSSKNELLLWTELMPSLYMISQMTDLSMAIMGTILF
                     LIIALGITNTLLMGLYERMFEFGVIKSIGTTPWQAARIMLFEAVCLGFVSIIVGLILA
                     LFVTALFANYGIDYTGIEFSGVTFQEKIYPSYKWSRLVLYPFMSLGFTLLASLYPAFK
                     LWKMLPVEALRKRKF"
BASE COUNT         7345 a         5883 c         5122 g         7577 t
ORIGIN      
        1 ccggtttcaa aacgtgtcat atcatacata tcaattggaa cacgcggatt tgttccaaga
       61 atttgttgcg ccaaagattc gccaattgtg ggcgccaatt tgaaaccatg tccgctgaaa
      121 ccaaccgcca catgcacacc ttcaagtttc ggaacaggac ctatgatggg attccaatcc
      181 ggaggaatgt catacggacc agtccaactc cgtaccagtt ttgcgtcggc aaaggcaggc
      241 atacggttgg ccattagttt gccaatccgt tcaatgtgtt ccatttccac atcgttcgtc
      301 atctcttcaa cattattaac cggatcgcct tgatctccag taccaaccag tactacacca
      361 ccagcatccg gtctaaagta aattttttcc ggagtaagta aatccttcac aatcggccag
      421 tctttttcat aaggtatgtc gattttcaaa gttagcacct tgtgtttgct gatttcataa
      481 gggaatttaa ttccaagcat ttttcccaaa ttatgcgtcc atggtccggc tgcaaggata
      541 agagtggagc attcaaaatc acttcgacgt gtttcgatgg ttttgtttac accgttgatt
      601 cggatgccgg taacaggagt tccagtataa aatttcaccc ccaaatcttt tgcacgcttt
      661 gcgtaagcta ttgtggttaa ataaggatca caataacctg atcgtgattc gaaaccaatt
      721 acatccacat cctcaaattg aagtaaagga tgaagtttag cagcttccgc tggagtcaag
      781 gtttgagttt cactaccata tttgttctga gtgcaaaata cactttccat cactggtcga
      841 tgcttttcct gtcccaaaac caattgtcct gtacaagtaa atcttggatc accacctacc
      901 atatcgtcaa agttttcaaa aattttcacg ctttctacag catgatgcac attggcttca
      961 attgaataat gactgcgtgt aatggcgcag gatttcgccg ttcctccgga acaaagttct
     1021 cccttctcca aaaccacgac attttttgcc ccttgattcg ccaaattgta ggcaatgctg
     1081 catccgatga ttccaccgcc gatgatgata atatctgctg tgttattttg tttcatagtt
     1141 cgtttgaagt gttattggtt ttgtaaaatt tacaaaatct gactcaaaaa ttctttggta
     1201 cgcgggtctt tggctttttt gaaaaaagtt gcgggtggcc cgtgttcgac aatgattccc
     1261 cgatctgtga aataaatgtg atctgccaat tcacgagcaa aacccatttc gtgcgttact
     1321 aaaatacacg tcattccttc tgccgcaagc tccttaattg taaccaaaac ctctttgacc
     1381 gtttccggat caagggcggc ggtgacttca tcgaaaagca tcacatccgg attcatggcc
     1441 agcgaacggg cgatggcgac tcgttgttgt tgtcctccgg aaagctcagc cggatagttg
     1501 ttttgcttgt cttcgagccg gactttacgg attaaggcct gggctttgac cagcacatct
     1561 tctttggctt ctttcaaaac caattcggga gccatcatga tattttccag tgcggttttg
     1621 tggggaaaaa ggttgtactg ttggaaaacc atgccgacat ttttgcgcaa ttccaatttg
     1681 ttcaaattgg agtcattgac ttccaagccg tgaactttga tggaaccgga attgatgtca
     1741 ttgagggcat tgatgcaacg aatcaaagtg gattttccgg atccggaagg gccgataata
     1801 caaatcactt cgcctttcat cacatcaaaa ctgatgcctt tgagaacttc gagttctcca
     1861 aaagatttat atacattgtt cagggaaaca atcggttgtt ccggagtcca gttatttgtg
     1921 gtcatgtcaa tctctcataa tttaacagaa tagcgttctt caagttttaa tgtccaacgt
     1981 gcaatcggat aacaataagc aaagaaaatt aaaagtgcaa agccgaaaaa tggaatcaat
     2041 aattcaggat gattgtcttc ggcttccatt gcctgacgcg atagcgtgac tatttcctga
     2101 acacctagca atgaaaccaa tggagtcgcc atggtcaaaa tcgcatacca gttcatccac
     2161 ggcggaatca tacgcttaaa acattgcggc agaataattt tccaaatcat ttgaacacga
     2221 ctgaaagcca aagactccga agcctcccat tgtccggtag gaacggactg caccgcgccc
     2281 cgaaccactt cagaaatatt ggccataatg ggcaatgaaa gcccgaaaac cgctttcatc
     2341 caatccggaa ttgaaatgat gtgctctcca atttcaattt caaaaggaaa agtcagcatc
     2401 acgataaaaa gcaacaccaa ccacggagaa ttgcgaaaaa gttgtgtgaa aaaccatgag
     2461 aatttcctca ccggagcaac gactgaaatt tgccccaaac cgagaaaaac tcctgcaacc
     2521 gttccaaaca acatggtaaa aaaactaatc aataaattca tcacaaatcc actgccgaca
     2581 acaaaaggca accaacgcca aagggtcgca aaggcttgaa tgagcgtgta ttttgattgc
     2641 gctagaacgg ccaatggata gaagctaatc aataaaaata tcaccaacaa aacccacggc
     2701 gaagaagaag ccaaccaacg gctgaaacgc aaatcttccg gttttccggc agagtgccct
     2761 tgatacggca ataataccgg caaatcgctt tgctgtgagt tgggggaaat tcccggaatg
     2821 tggtttaaat aaccgaacat tgttttttaa ctcgaataaa tttaaattaa aatattgaaa
     2881 taacaatcat tattttcgtg atgcgagtcc ttttggatcg taaggcgcat ccagtgaaac
     2941 ttgagcggga aagtgcctgc gagcaatttc aacctcaaaa ttaccagtgt tcagcattgt
     3001 gtccagtttc gtttccaccc agccgatgta acccatcatc acggcgcaac caagtgtatg
     3061 cccaaaagtt ccggaagtgg tacgcccaac taacttgtta ttccaccaaa tcggttctcc
     3121 gccgtgcggg aatgcttctg aatcggcgac ggtgagcatc accaaatggc gggtgtctcc
     3181 ttccgttttc tgctttaaca gtgcttcctt tccgataaat gattccggtt tattgaattt
     3241 caccgcaaac tcaagtccgg cttgcaatgg tgtatcgtcc ggcccgattt catgccccca
     3301 cgcacaataa cccttttcga tgcgtaagga actgagtgcc tcgaccccag caagttttaa
     3361 ccctaaatca tcaccaaccg tgctgagtgc gtcaaaaacc ggaagtgcag attcgctcgg
     3421 aatatacaat tcccagccca actctccaac gtatgataaa cgagccgcac gaaccatcgc
     3481 cggaccaacc tcaatggtgc ggtgtgtgta atacggaaaa ccgtagtttg aaaaatcgtc
     3541 taaactgaca cgactcaata actttcgtga atttggcccc gcaatgctga ttactgcata
     3601 agctccggta acgtcggtaa cggtgacaaa ctcgtccgga ttgaggtttc tacgaatcca
     3661 gtctttgtcg cgcaccggtt gtccggttcc ggtgacgagc aaaaaactat ccgcatctaa
     3721 gcgcatcaaa gttaaatcac tttcgtaacc gccatgcttg ttgagcatgg ctgtataaac
     3781 aactcggcgg tctgctttgg aaatgtcgtt ggcacataat ctttgtagaa cagattccgc
     3841 gtcacgtccc tgcaccagca atttcccaaa agtcgattgg tcaaataaaa caacagcttc
     3901 tcttgccgct cgatgttctt ccgcatgaag tgaatgccag ccgggtttct caaaactcaa
     3961 ctccggtgca gtcggtttgt tttccggatt gaaccagcga ggccgttccc aacccatccg
     4021 ttccgcaaat tctgctccgg cattttcgag tcgggaatgc aaaggactaa gtcgcaaatt
     4081 ccgagcagtt tgatgctgtc ttccgggaaa actgacggcg taatgcaaag cgagcgtttc
     4141 cggagaacgt tcacgaagtg tccgtaaagt attttgtgcc ggaacaaaac gccgaatatc
     4201 taccggccaa aggtccatcg acggctcgcc gtcaattatc cattcagcaa gtgcgcgtcc
     4261 ggccccgccg cccgttgcaa tacctacaga acacattccg caaccaagaa agaacccacg
     4321 cagttccgga gattcaccca acaaaaaacg gtcatccgga gtaaaacttt ccgggccgtt
     4381 gagtaacatt ttcacaccgg tttgctccag ttccggaatg cggtgcatgg cgtttgcgag
     4441 aatcggttca atgtgatccc aatcttccgg aagtaaatcg aaagcaaaat cttcaggaag
     4501 tgtttcaatg tctaaggctt ttccttgcgg ctcaaagcaa cctatcagta accccccacc
     4561 ctcatcccgc agatagagat gtccgtcgtg gtcgctgagt gtagggagcg gagccgtgac
     4621 cgaatccatc agttcggtga gtaaataaaa atgttcacat gcgtgcagtg gtgcgacggc
     4681 tccaaccatt gtggcaatgc tacgtcccca aattccagca cagttgacaa ctgtttcaca
     4741 actgatttcc ccttgtgcgg tttggacggt ggctacacgt ccattttcag tgcgaattcc
     4801 agtaactggt gtatcctcga aaaccttcaa cccctgtgac tttgctcctt taatcagtgc
     4861 ggcgcaaatg tcgctcggac tgacccgtcc atcagagggt gaataaactg caccgatcag
     4921 atcatcgcta cgcatcaacg gccatttttc agcggcgttg ctcactgaaa tttcctcggc
     4981 accaattccg aaagcttgcg aaagagacgc ttgtcggcga atgtgtgtga ggcgatccgg
     5041 attggtggca atgctcaaac ttccagtttg tttccatccg gtggcctgtc cggtttctgc
     5101 ttccagcgag gaatacaatg ccacgctatt ctgcacaagc cgtgtgagac tttcggtaga
     5161 tcgaagttgc cgaacttgtg cagcggaatg ccaagtggtg ccgcttgtta atttcgtccg
     5221 ctccagcaaa attacatctc tgcaaccatt ttttaaaagg tggtacgccg tgctacaccc
     5281 catgatgccg ccgccaataa ttacgatttg gctctgcttt ggtaacttag tactcattaa
     5341 acttgagttt ctgaattagt gtattctact tgagatttca ggaacccaca tcttgaaaac
     5401 attcatcctc cataacccgg aattcgactg ttttcctccc atcggctcat gcccaaaacc
     5461 agaatggcaa ccaggccgat atacacgaca aacaacaaca gcatcgaagc attcatggct
     5521 gtcgaatagt cgttgtaaat gctcaccatt tggtaaagaa gttccggaac tgcaatggcc
     5581 atagcttgcg ttgtggtttt gacaaggttc accaaattgt tgttcagaga aggcaaactg
     5641 acccgaaaag ccaacggcaa aacaatgtaa cggtaagttt gtaaatggct gaaacccagt
     5701 gcttcggagg cttctttggt agattccgga accgcctcaa ttccagcccg gaagatttcc
     5761 acattgaaag ctccggcaaa aaacgaaagt gaaataatag cccagcctac attggaaata
     5821 ataggaattt ccagccaacc gtccggagaa taagtcggtg taaactgacc cagtgcaaag
     5881 tagaaaaaca tcaactgaat caatggcgga gtgttacgga aaaactggat ataaccctga
     5941 accagcaagc gtaaaaatcg gttgtgctgg ttttgcatcc atgctccgac aatcccaatc
     6001 acaacactga tcaggacaca aaccactgcc agttcaatgg tagtccaaaa accttcgatg
     6061 acacgatcca tctgaaccgg atcatagaaa aaaatgaagt tccatttggg atcgcttttg
     6121 taaagctcca aaaagaaatg gctgatggct tcgggcatta tgaatatttt tcggggagaa
     6181 ccggaacgtc cggtgcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     6301 ggaacgtccg gtgcgggtga aagcgaccgg aactttttta gttcaagtcc ggtcgccgga
     6361 aagaaaatgg aaaccagtca agatttatct gcaatgctca actggtttgt gtttgattta
     6421 tttgccttcc aaccagtctt tactacgaaa cttttggatg acgagataat ttgtagcctg
     6481 aatgccgtgt tttttctcca gttcgatcaa acgtccggac tgatgccagt tgtatgtcat
     6541 accggtcatg aagttgccaa atacacaact tagctcctct aaaggaaccg caagtcccca
     6601 ggggttatcg tcttcggaag ccaaaggcat ttcaaaatcg tcccaatttc cggaagatag
     6661 atccgacata atggaggaat cgtcataaac ccaggcgata cactttttat cgcgcaaagc
     6721 ttgtttagct tcagcgtttc cggtgaaggc gatgattttc gctccataac gatcggcgac
     6781 aattttgtta tagaatgccc cttgtttgcc gcaaaccggc ttgccgcgca gatcttccca
     6841 agaggttaga cccaaggctt tcggagacat aatattagtt ccggaagtgt aatagttagg
     6901 ctgagtgatg ccgacaattt tacggcgatc cacacgatcc gacatggtgg cgatcatcat
     6961 gtcggttttg ccctgttcca agaactgcat ccggttcgag gactgcaccg gaaccaattc
     7021 cagtttcacg cccattgcgt cagcgacatc cttggccatg tcaatttcca tacccaccag
     7081 tccaccggcg gtattgcgat aaccccaagg tttgtaatcg gccttaacac cgatgacaat
     7141 tttaccgcgc ttcattactt tgttccatct ctcgttcgtg cattgtccgg cactagccgc
     7201 agtggaaact aggcttaaag ccaaagtcgc cgacaaagta cccaaaatca ttttattcag
     7261 tactttcatt ttattctcca ataagagtta aaaaatcttt gcagcaccat tgcgcaaaga
     7321 taaaatgcta cacagaacga aaattcaaat actgaaaaca tcgttattga aattgtgtaa
     7381 catccttgca cgcaaagtta taagtttatt agctgaacct gtcaagtact tttcatatat
     7441 ttcttcacta gtgtcccaat aagttatcga ataaattcaa ttggaaagaa tcttcaatgg
     7501 caattggagt attattatat taatcaagta actgaattaa tgatattttt caaataatga
     7561 ccggcttaaa acctgtagaa tattgtagag tgatgcatct attttcagtc gttttttgat
     7621 aagagccaca ggcacataaa cagaaactgt gatccggatt tgtgttttga ctgcgttttc
     7681 agaggttccg taaaactatt tgatgcgtaa gtgcgtattg ttagcattcg agatagatta
     7741 gctgttttta aaataatatt ttctctttac acttaagatg gattcaataa ttcctagtac
     7801 aggcgtaaaa tagcttatgc ttataggaca tctggtacat tagggtcaaa gacacttttt
     7861 tatttttatt tccagctctc tcaataaatt tttcaaatac aactgaagtt tttctgccct
     7921 atcttcacaa aatttccaag gcacaaactc atccatataa gtccgttgtg caatttccag
     7981 ttgaatacaa tgaattccat attctggatt tgcataagtc cgggtagtcc aacccccatt
     8041 gaaacgtcca ttcaaaacgt aattgtatct gccatccttt tcacacatgt cgattgctac
     8101 tttttcaatt tttgaatcac aactcttacc attgtttgta cctaaattta attccggtaa
     8161 tgcaccttca aataaatttg gcaggtatga gcggattgaa tgacaatcga aaagcaagac
     8221 aatgccgtat ttttttttga tacgattaag ttgttccaac agtgcagcat gatacgcggt
     8281 atggtaagtt tgtagacgac tttcaatttc ctgagcatct ggttctgctc ctttattata
     8341 aatgtcctgc ccctcaaaat ccaatgtcgg acatagttca gttgtaacca gtccaggata
     8401 aagtgatttt ccagaaggat cacgattcac atcaattaga tagcgattaa aagtggcctg
     8461 taccacagtt gcttgtggaa gcagaccctt gtaaagtcta tgaatatgcc agtctgtatc
     8521 agctatctta agaccgttag cattgaattg agaagcaact tttgcaggta taaatgtccc
     8581 tccgtgaggt tgtcctagaa tcacagggct ttctccttca aatacttcaa ctggattcat
     8641 gactctccaa tttttgaaat ttgtattccc acacaaaatg taaaaaggtt tattttctga
     8701 atcagattta atttttaatc aacttaatct ctctaacttc atcatctgca tttcaattta
     8761 aattaacatc ttgctttctt tttgtaagta catataattt ttttctcaac tcaacaacta
     8821 tgacaaaatg aaatattttg ctgaattagc tctcattccc gaaggatggt caaagaatgt
     8881 acgtgtgaca attgattcgt cgggcaagat tgaatcagta gaatcaggga ttgaacctaa
     8941 tcatgaagat atacatctaa aaaatcgaat ccttctacct gcattatcga atctgcatag
     9001 tcactctttt caaagggctc tagctggatt gaccgaaaag cgtttgaaga ataatgacag
     9061 tttttggagt tggagggtgt taatgtatca ttttttggaa caactttctc cagagcaact
     9121 tgaagcgatt gcctcacaag tatttatgga gatgctggaa tgcggatata cttcagtcgg
     9181 cgagttccat tatgtacacc atcaggaagc cggaaagcac tatgataata tagcagaaac
     9241 atcttgtcgg ataatcactg cagctcatga aacaggaata ggattgacac atctgccagt
     9301 tttttatatg caaagtaatt tagacggatc taaactttct caaaaccaac ttcgctttgg
     9361 caataattca gtacaatatt ttcgtattct tgaactagcg cgagctgcac tcagtaatgc
     9421 accgaaagat tatttgctag gaatcgcacc acattctttg cgagcagttt ctccagaagc
     9481 cttggtagaa ataatcgaag cgaagacctc cggaccggta catattcaca ttgctgaaca
     9541 gctcaaagaa gttgttgact ttcagcttaa attcaacgct cgcccggtgg aatggttgtt
     9601 ggataatact gcagttgatt caaaatggtg ttttattcat gcaactcatt taacctcaaa
     9661 agaaatacac aattcggcta aatccggagc agtagcagga ttgtgtccaa tcactgaagc
     9721 caatctggga gacggaatct ttgatggttc cggctggctt ttgtctgggg gaaaatatgg
     9781 aattggttct gattcaaatg tacaaatttc actaacagaa gaattacgat tattagaata
     9841 cagtcaacga ctcttaaaaa aagaacgcaa tgttatgtct caagtaccag gatctgtcgg
     9901 gcatgcactt tacaatgatt ctctagtagg cggagctcaa gctttgggcc gtaattccgg
     9961 aagtataaca tccgggaatt gggctgattt gctgacactt gatgcagaaa gtctgacttt
    10021 ttgtggatgt actgaagatc aaattttaga tcgttggatt ttttgcggta acgattccct
    10081 tgtccgtgaa gtctggtctg caggtcggca aatggtggtc aatggtcgcc atattaagca
    10141 cagtataatt gaaaaacaat accgaaacgt aatccaagaa attcaaaaag aaatgtgagt
    10201 gtgtttccaa acttaaattt acttgtccac tccaaagatt acatagaaaa tattagatat
    10261 aattctttga tccaaatatc aacagcctcg tcaataacca cattgcctgc gtcaattcta
    10321 taaaatttat agccaaattc attgaaaata cttagccggt tttgaatact agataattca
    10381 gactttcaag ccttaaaaac aaaaaatccc gccaaagtta tgtagaaaca aactctggcg
    10441 ggataatttt agcaagcaga atcagcttaa gattaattta atttcttaag atctgactct
    10501 gttggctgac caatctcgat catgcgagat ttcttttctt caggaacgat cctttcaaga
    10561 ctaatggtca gcataccatt aaccaaatcc gctcctttaa ctatcacgtc atcagaaatg
    10621 gtaaaactcc gtgtaaatgc ccgcttggca atcccacgat gcaaatattc tccttcttcc
    10681 tgatctgttt ttgaacgcac agttaataca ccttctttga gctctgcttc cagatcctct
    10741 ttggaaaatc cggcaacagc catttcaatg gtgtacatat tttcttcgtc cttacgaata
    10801 ttgtatggtg gaaaaccgcc ctggttttca gacctttcaa catctccgaa aagacggtca
    10861 aacattgaat caaacccaac ggtaatcccg agagctttat caaaatctga cggtgttatt
    10921 gatgagtaac gtgctaaatg taacataata cctccttata taagcaaggt gttaacagaa
    10981 atacctccaa tccatagcac tagacctgga ggtggttgaa ggggctaccg ccatggtcag
    11041 ccccggactc ggcttctctc acttttcact aagttagaga accgaaatta aaaaactatt
    11101 taacggctta ttcctcattg gagacagaag taatattttc aaaaaatatt ttagctttgg
    11161 aaacatttgc tgtgacttcc tgtccgtcat ggatttctcc tgataaaatt gctcttgcca
    11221 aggcagtctc aagttctttc tgaattgcac gtttgagcgg tctggcacca tagaccgggt
    11281 catagccgac ttctgcaagg taatcgatag ttttatcttc caactgcagt gaaatgtttc
    11341 gctcttctaa acgctttttc agcctttcca actgaatctt tattataccg ttcatgtgtt
    11401 cctgaagcag cggatgaaag acgactgtat catcaaccct gtttaagaat tccggacgaa
    11461 aatgctgccg cagttcctgt agaaccaatt ttttttgttc tgccagcaaa gatgatccgg
    11521 aaattccgtc ctcattttgg ttgatcaatt tttgactgcc aatattcgaa gtcatgatta
    11581 ccagtgtgtt tttgaaatca acggtacgtc cgtgtgagtc agtaagtctt ccgtcatcaa
    11641 gaatttgcag caaaacatta aaaacatcaa agtgagcctt ttcaatttca tcgaacaaca
    11701 aaacgctgta aggttttctt cgtactgctt cagtgagttg tccaccatcg tcaaagccaa
    11761 cgtatccggg aggtgcgcca atcaaacggg aaaccgcatg tttttccatg tattcactca
    11821 tgtcaatgcg gatcatgttc tgctcatcat caaataaatt ttctgccaaa gcccgcgcca
    11881 attctgtttt acctacacct gttggaccca aaaatataaa acttccaagc ggcttgtttg
    11941 gatcattgat tccggagcgt gcccgcatta ctgcatccga aacaagatca atcgcctcat
    12001 cctgtccaac cactcgttga tgcaactgtt ccggaagtcg caacaatttt tcgccttctc
    12061 cttcaatcag tttctgcaca ggaattccgg tccagtgtcc tacaatttcg gcaatgtcct
    12121 ctgcatctac ttcttccttg agtagttgat tcttgctgct ttgttcttca gattgtgtgg
    12181 ttttcagcct tttttccagt tccggaagtt caccatattc caaacgtgct gcctcttcca
    12241 aattatagtc ctgccgtgca cgttcaattt gcagacgcac ttcttctatt tcctgcttca
    12301 atccactcat atcctgtaaa gcagattttt ctttttccca ttgcagagtc aaattgtcaa
    12361 agctgtgctg caactctgcc aactctttag acagtttttt cagcctctct ttggaagcag
    12421 tatcattttc ttttttcagt gcttcttttt cgatttgcaa ttgcagtatt cgccgtgata
    12481 tttcatccat ttcagcaggc atggaattta tttctactct taaacgcgct gccgcttcat
    12541 ccatcaaatc gatggcttta tccggaagaa agcgatccga aatatagcgg tgagaaagcg
    12601 ttgcggcggc aatcaaagct gcatctttta tacgaacacc atgatgtatt tcgtaacgtt
    12661 cctttaagcc ccgtaaaatc gaaatcgtat cctcaactgt gggctgatcc accaaaacct
    12721 gctgaaaacg ccgctcgagt gcagggtctt tttcgatatg ttttcggtat tcatccagag
    12781 tagtggcacc aatacaatgc agttcgccgc gagccagcat tggtttaagc aaatttcctg
    12841 cgtccataga accttcagtt tttcccgcac cgacaactgt atgcagttca tcaatgaaaa
    12901 gcattatttg tccatctgaa tctacaactt ccttgaggac tgctttaaga cgctcttcaa
    12961 attcacctcg atatttcgct ccggcaatca gggcacccat atcaagcgaa accagctttt
    13021 tattttttag actgtccggc acatcatttc gaacaatacg ctgtgctaaa ccttcggcta
    13081 ttgcagtttt gcctacaccc ggctctccaa tcagaaccgg attgtttttt gtgcgacgcg
    13141 aaagtacctg tatcactcta cgaatttcat cgtcccgacc gatcacagga tccagctttc
    13201 catcacgtgc acgctgactc agatttgtcc catatttttc aagtgcctcg tatgtttcct
    13261 caggattctg acttgtcact ctctgtcctc cacgtacatc ctgaattgta cccattaaac
    13321 gttcacgtgt aactccattc tcagccaata tttttcctgc aggtccctta cgtccctcct
    13381 cggtcaatgc cagcaaaaga tgctcaacac ttacatattc gtcctgcagt ttttttgctt
    13441 catcttcggc tcgcaccaag gcctgattca tttcaccgct agcgcccact cttcctgctg
    13501 aactcccgct aagagtcggt aaagacttca aagatgattc cagtgcctgt gtaaattttt
    13561 cagaatcgag ctccattttt tcgaaaatcc tgggaactaa accattttcc tggaaagtta
    13621 aggcactcaa aagatggact gtggacaact cggagttttt gcgacgtact gcgtcgctgt
    13681 gtgcattttc caaggcagaa agcgattttt ctgtgaattt ttgggtgtcc attataacct
    13741 ttattttttc gaataaaatc cttttagcac tcactatact agagtgctaa caaaacttca
    13801 agtgaaattt tttacaaaga aaaaaaagtt ttccgggaaa ataattatga aatttatttt
    13861 tttttgtttc ttactttttt tgtaataaaa tccaaaaaag caataaaaat aaaaaaatca
    13921 gaggtatgga atgaactaaa aaaaatagtc ttcaagtcag caaaaaaata tttcaaaaaa
    13981 atattttttt ttataattcc tgcttgaaat ctgtttggga agtctgtatc ttttgaggct
    14041 acaccccgga gtgaatatgg agcaaatccc aggtcatgct aggctcagga tttaaattca
    14101 tgaatttgta aaaggattca agtggcggag ccactctccg gggtcttaat aaatatgagc
    14161 aacaagcgtg ccttgcccct tccacaatgc tttcatgtaa tttctgcctt tattattttc
    14221 ttatttgttg ctaaaattac ctcttttaga ttttccagaa gaattatact tagctcatag
    14281 atttttctac cattcgtcca aagaaaagaa tggtgttttt tgtgaccatc atttttttca
    14341 ctgacacaga tggccaagct gtatttttac tattctgcaa tgaatgcagg aaaaaccacg
    14401 acactccttc agtcagcata taattatcgt gagcgtggaa tgaaaacact ggtacttaag
    14461 ccaaaagttg atgatcgtta caaccccgac agggtacgtt cacgaattgg actggagtcg
    14521 gatgcagcca gttttgagtc agaagatgat ttacttgctc tgacagagaa aattgacaaa
    14581 aaaactcctt tgagctgtat tttgcttgat gaagctcaat ttttgacaaa aacccaggtt
    14641 tatgcattag gagagattgt tgataaactt ggaatccctg tactggccta tggtttgcgt
    14701 actgatttta agggagagct ttttgaagga agtctgcatt tactggcgtg ggcagacgaa
    14761 ctggtggaaa taaagactat ctgtcactgt ggtaaaaaag cgaacatggt acttcgtctt
    14821 gatgagaatg gagagccgtt aaagtccggt gagcaaatac agatcggcgg aaacgacagc
    14881 tatgtttcag tttgccgaaa acattataag tcagcaggaa cctgaaaact tctgaactga
    14941 tgccccttaa ttctgtaact attaatgtat acagaattca gtggacactc attatcaatt
    15001 agtaataaca aaatatttta atattccttt cacaaaaaaa taaatttgaa aaatgttgcg
    15061 attgccggtg caaccggtgc tgttggtacg gaatttctta aacttttgga actccgtaaa
    15121 tttcctatta acaatttaaa actgcttgct tccgaacgtt ccgttggaaa gaaacttgag
    15181 ttttgtggtg aacaaatcac tgttgaagaa ctgacgacaa aatcttttca aggagtagat
    15241 ttggtgtttt tttctgctgg aaaagggcgc agcaaagaat ttgtacctca tgcagttgat
    15301 gccggtacag tggtgattga caacagcagt gctttccgat tacatccgga tgtaccgctt
    15361 gttgttccgg aaatcaaccc gcaaatggtg ttcaaacaca aaggcataat tgctaatcca
    15421 aattgctcca caatccaaat ggtggttgcg ctcaaccctc tgcaccaggc cgcaactatt
    15481 aaacgagtgg tggtttctac ctatcaatcc acttccggag caggttcaaa aggtatgaga
    15541 gagttgatca gtcagactaa agcctggtta aacgatgatc ccttggaggt ttcagcattt
    15601 ccgacacaaa tagctttcaa tctttttcct catgtagacg tttttctcga aaacgggtac
    15661 acaagggaag aaatgaaaat ggtcaatgaa acacataaga ttatggatgc accggatata
    15721 ggtatttctg caacttgtgt cagagtcccc gtcctgcggg cgcattcaga ggcagtttgg
    15781 atcgaaacag aacagaaatt atctccggaa gaagctcgtg aaattctaaa aaaagctccg
    15841 ggcataatag ttaaggatga accagttgac ggaggttacc caacaccgtg ggaggcaagt
    15901 gaaacatttg agacatttgt agggcgtata cgtgaagact tttctcatcc taaaggcctt
    15961 acattttggg tggttgccga tcaactatac aaaggcgcgg ctctcaactc gattcaaatt
    16021 gcagaagttc tggcaacagg tcaggtaact tagatgatta aataaatttt actcgccttt
    16081 ttgcagcttt aattaaactg ggatccaaga gaaatattat gcgaatattc tttctcttca
    16141 aaagcatatt atttatttca gccttaagtc tcactgccac tttggttcct gctcaagaca
    16201 gcagtccagc cattgcaaca cttttacaaa ttgagggagg tgtagaagtt gtaaccgata
    16261 aatctccaaa aggccgacga gggagagacg gaatgctgtt gtttgccgga aataaaatca
    16321 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nactgatgat ttgtcaaaaa
    16441 aagtagttcc aattcctaat ttgctgaatc taaaaactaa tgagtatgaa ttcgacttta
    16501 ggaatggaaa ttcaatacag ctcgaattca gtgttcagat tcagaacagt gtcagcagta
    16561 agattgtcaa acgcaaaggt ctggtcattt ttgaaagtga ttacaaccgt atccgtctgc
    16621 ctaaacgttt tatgttggat gcaaacggtt atgcccgagt tttagttggg attgatcctc
    16681 cgcgtattga cgattcaaaa tttagaggtc tgattaccat ccgtgcattc atggatgaag
    16741 atggatttga tgatgtggct gaaggtaatt tggtactgaa aatacgtaat tcaggcaaaa
    16801 tacgtacact cattctcgat ccagacaaag gtttaacaga aacagagggc tgacaatttt
    16861 tctcacttcc gcagcaaaat caggtgttcc cacttcccgg tttttccgaa tgatttacgc
    16921 agctctgaat catatatttc acgctgaact gaagcacctc cagtcacttt ccaaccaata
    16981 ctgtctgcgt acttttgcgt aaattccaaa ccactaacac gtttgttacg ctcaacccag
    17041 tctcccaaca ccagataaca tagaccgtcc tcagcagtcc atctacgcaa tttcagcata
    17101 aattcccgaa aaacctgctt ccattgtcct ggtgaataat tacggttcgt aatttcacta
    17161 gaagacattg tatttgaaga taaatccagc cacttcatcc gcagctcgtg tagtttgaga
    17221 tagtcatatg ttcctggaaa aggtggactt gttatcgtgc agtttaccga gttttcttcc
    17281 ggtccctcca attctagaat gttttcgttc caaattattg caggtgcagt attcggaatt
    17341 tttgttgcaa gtttcagctg atttttcaac aattcctgtg tcttgcgttc catccatttt
    17401 ccgactgcac cttttggaaa agaaggttgt ttggtgtttt cagaagtctc aaaaattttt
    17461 ttggagaatt taacgatcaa acttgaaaaa acagcaacta aagtttcacg ttcatttgcc
    17521 gagtaaagat tttcaatgcc gtcaatccaa tgcaaaagtt ctacaaacaa atatggtgga
    17581 tggaaatgac tgagccagct taaatttcgt cggtgaacac tttttttcgc cagacttctt
    17641 atattaactc tttcccgtac atcttccaaa gcactccata cacggcttgc atttttttca
    17701 gaaatccagc gacaacgttc ccttgtaacc atttttgcaa taggattgag atcgtttccg
    17761 agaactttgt gcttccaaag caaaccctca agcaaaacag tacctccacc cataaatgga
    17821 tcaaaaacag tacttccttc aggaaattgt ttgagcaaaa tctttggcaa atcgggatgg
    17881 aatcgtccag gataaggatg aaaaccttgt gtaacatcgt attcgcttcg ccctttcaca
    17941 gaagctccca gcgcttccag catcagtccc tcctgttccc caaaagattc actgttcaaa
    18001 aacatttgcg atgcagatct tcgctgtttt attttttttt cattttttgg cggtgctcca
    18061 aaagtgtttt cactttcagt ttgctgccag tcttttcttt tccagggaag ttgagcagag
    18121 ttttgagttt tgagttttga gttttgctta taattgcttt ccggctttga gactttaggt
    18181 cttttatact ttgaatctcc ggcattctct tccggagcag cttttgattt tttcctgaca
    18241 cgtataggtt ttttatccaa atccgcagca gtaatgggga tagttccggc gctagacctg
    18301 gccttatctt tgatgctttt tttttcagta tcctgcatat ttattttcaa acttttctta
    18361 ttatttggat acagcttgtt ccgggatttc cttttgttca gtaccgtaaa ttatgaaaac
    18421 ggtagtgctg attattaaga cacctccaag caaggtttgc aaagaaggaa tttcttccag
    18481 cagaaaaagt gcaaacaaaa tcgcaaaaac cggttccagt ccggcaatca acaaacttgc
    18541 cacttgagca cgaacatgac gcaagccgtt gatcaacaag gtgtggcctc ctactgtaca
    18601 aactacacca agaagaaaca ataaacccaa ttcttttccg ctgagaatcc atgattcaga
    18661 taaagtccag ggcaatatta ccagtgcagc aaaaccattt tgccagcagg ttatcaataa
    18721 cgcagagtgt tttctgacgt gtccccgatt gagtaaggta agtaccgcaa agccaaatcc
    18781 tgccatcaaa ccccataaaa ttccatgaaa aacagaaccg gatataattg tgtttgaatc
    18841 atcagaactg gaggtagcca tcagtgccaa tccgcatatc acaatcaatg ctgcaaagac
    18901 atttcgtacc cgaagaggtt cacggaaaaa cagtggttcc agcagggtgg tgaataaagg
    18961 gtaactggca aatgagagca aaccaattgc tacagtggaa acttgaattg cctgaaaaaa
    19021 tgcgatccag tgtaccccca atatgcttcc gctcagtgcc agccagagcc attcccgatg
    19081 ttcactgaaa aagatttttt tacgcaataa gaacagtgcc agcaacaatg ctccaaaggc
    19141 aaaaatagta cggccctgta cgatcagtaa tggagagaaa gttagaaact tgccaaacaa
    19201 tcctgtcagg ctgacaaaag ggagggcgac caaaacgctg atcaccccat ggcggtgcga
    19261 cgttttagcg ctggtgaaat ccgacatcaa tcagtgttaa gctgcttgtt tggtacgtaa
    19321 atatacccgt agaatcgtga tcgctgcagg cgtcataccg gttattcgac ttgcctgtcc
    19381 aagtgttccc ggttttaatt tatctaatat ttcaacaact tcacgagaaa gtcccggaat
    19441 tccggataag tgcaaattgt ccggaatttc aattcgatcg agttgattaa cgtactttaa
    19501 ctcctgatct tgcctggcaa gataacctgc atacttaatg ttgatttcta cttgttcccg
    19561 cactaccgga gaataataat tccagtctat tgatccactg atggattcac tgccgagcag
    19621 cctgtcaata ttaatttttg gacgtttcag caaatcggaa actttggcag cagttttaag
    19681 gggagcttct ccaagttttt tcaattctgt ttgaactgct ttattcggcg taattcgttc
    19741 tgcttttact attccggaca acctggaaat ttcctgctgc ttttgctgaa aatgctgaaa
    19801 atatttttcc ggcagcaacc ccaggtccca gcctttttgg cttaaacggg catcggcatt
    19861 gtcttcacgc aaatgcagcc tgtattccgc tcgtgaagtg aacatccggt atggttcaat
    19921 cactccgcgt gttatcaaat catttatcat cacaccaatg taagcttcat ttcgctgaag
    19981 aataaacggt tgtttttttc tcactttcaa aaccgcatta attcctgcca tcagaccctg
    20041 cgctgcagct tcctcatatc ctgtagtacc gttaatttgg cctgcaagat acagattttt
    20101 cactgttttt aactcaagtg tcgctttcag ttgagttgga tccagcatgt cgtattcgat
    20161 agcataacct ggttcgaaaa tttcaacttc ttccaagccc ttgatactgc gtaaaaactc
    20221 aaactgtact tctgtcggca aacttgtaga taatccattg ggataaatca ttaagctgtc
    20281 ttctgcatat cccatcggtt ccatgaatac ctgatgtcgt tttttgtctg caaacttaac
    20341 aattttatct tcaatagagg gacagtaacg gggtccaatt ccagttattt ctcctccata
    20401 aagtgcagac ctgctcaggt ttttttggat aatcaggtgt gtgcgctcat tcgtgtaggc
    20461 aatgtgacat ggtacctggg gcagtaaaat ctctgaatcc caaaaagaaa actttttggg
    20521 cttttcgtca ccaggctgaa ttcccagttg actccagtca atagtacgtt tgtcgagacg
    20581 agcaggcgta cctgttttca tccgtcccag cttcaaagac tgatcagcca gggacagtga
    20641 aagtcgtttt gcggaaggtt ctgccacacg tccggcatca acacgctctg aaccacggtg
    20701 aataaggcca ttgagaaatg tgcctgtggt gatgattatt gtttctgcat ataattcctc
    20761 tttttcagta cggagaccca ccacctgatt cccgtttaac aacaattcct ctgcagcagc
    20821 ttcaataata gtgagtccgg actgtttttc cagtatatga cgcataaatt ttttgtaagc
    20881 cagataatct gactgacagc gtgaaccatg tgtggcggcg ccgcgggatg tattgagcac
    20941 acggaactgg atacctgttg catcggcaac tttccccatt tctccaccca gcgcgtcaat
    21001 ttccttaacc agatggcctt taccgattcc gccaattgca ggattgcagg acatttgcgc
    21061 aatcgtgtca cgggacagtg taacgagcaa agtatccgct cccattctgg ctgcagccag
    21121 tgcggcttcg catccggcat gtcctcctcc tatcacgaga acatcgtatt ttttagggtt
    21181 agttttcaat aaaaatccta ttttgttttc gttatcgtcg gacgtcccaa ctctttaaag
    21241 gcccggccca cgacaaaatt attgtgtatg aagtaacaag tatagctgaa tgcttcagaa
    21301 ctattaagat catgtagaaa gattttttat tccaagacaa cttttattct ggaaaaaatc
    21361 tcatccggag ttccaattcc gtcaattgaa acgagaatac ctttttcttc atagtacttg
    21421 actaggggtt ttgtttgttc atgataagtg atcagccttg attgcacagt ttcttcgtta
    21481 tcatcgtccc gctgaacaag ccgttcacct gttacatcgt cttttccttc aattttcggc
    21541 ggattgaatt caatatggta actgcgtccg ctggccaggt ggaagcgccg tcctgcaatc
    21601 cttgtaacca ccgtcttatc atcaacacac aactcaatcg cattgtcaat ctgctcattt
    21661 tgggcagcca gcatttcatc cagttttttg gcttgagaca aattgcgtgg aaagccgtct
    21721 aacaaatatc cttttgtaca atccgcatgt gtaatttgtt cacggacaat tccgatgacg
    21781 atttcatctg aaaccaaacc tccggaatcc atcactgttt tagcttgttt accgacttca
    21841 gatccggatt tgacggcagc acgcaacatg tcgccggtgg aaagttgtac gagaccataa
    21901 tctcgaatta aattttgagc ctgagtacct tttccacaac ccggcagacc tagtaaaatt
    21961 atgtgcatta atatctttct tctgaaacat tccgttaata ttgaaaatac ttaaaactca
    22021 tcttatccat taatttagag gataatttat tgcttgagtt tctgaaacag actgttagat
    22081 acggatattt tctaggcctc tgattataaa gcatgcagcc aataagacaa attaaaatca
    22141 cttcagttct tcaaagtgtt tcaaaaacac aattcatcat tacttccgga actacggatt
    22201 catttggaag caaaagcagt gtgttgacaa tttgagcaat gtcttccggg cgagacatct
    22261 tttcctgcgc aaaagtcttc acctcagccg tcatatcagt tctcacaaaa ccagggcaaa
    22321 ttgcagttac acgaattccg tcatcccaac cctgcagacg tgcagcatgt gatgcggcca
    22381 ttgctgcgaa tttactcatt gagtatccaa ccaaattacc tttgactctt tttccggaca
    22441 tggaaataat atcgataatg cgtcctgaac ctgattttcg caaataagga aatgcgagtc
    22501 gagttaaacg cagaggagcc ttggcgttga cctcccacat gtcgtccagc aaatcttctt
    22561 catcatcttc cagaccggta gcatgtaaaa taccggcatt gttgaccagt ccgtcaatgc
    22621 gtccaaattg ttttacagta gcatcgatcc aggttttagc agaatcatgg tttcgggcat
    22681 catactcctg acagagaagt actgtttccg caacagaagc tagtgtttcc ggaagttgtt
    22741 cgggattgcg aacacccaaa ctcattcgat aattttcgct taagagtttt tccgtaattg
    22801 cgcggccgat tccccgattt gctccggaga tcataattac tttttttgct gagttttcca
    22861 tgagtttccg tcttaacgat aatgttttag aattaattta aacgagttcg gtgtgtctcc
    22921 aaattcagaa tttttttctg tttgcatcga ggattttata ccggaagcca aactgatgtt
    22981 ccaggacaaa agcaaatctt ccaaaaagtt ccattccagt ccggaagtgt tcaaaaacga
    23041 gctgtcgttg aggttaaaaa atgcagaact actgaatgac aaaagcggcg aaaactgata
    23101 gctgagattc ggcagcaaat aatccctgcc aagcagcgaa accggaaatt cactgtagat
    23161 cgttgaggat gagttactca catagtttcc gggatttttt gctcccattg gattatgatg
    23221 aaaatcgagc atcgtaaaca aattctcatt ccattgaaaa ttcagtccta tagtccaggg
    23281 cgcccattca tttctaactt cctccgtagt ttccggagca gattttctct gcaaaatcaa
    23341 cgctccatca aataccagtc ccagcattcc taaatctgtc tgcaaagtca ggccaagcag
    23401 ttgattttgt tgaaaacttg ccagcagcgg tttcagatcg gttgttccca gcaaaaaatg
    23461 tgaacttaaa taagcaccgt tgttttctga agacaaatct tttcctgcca aaattcctgc
    23521 ttcaaattct gtcgtttctc ctaatccacc aacaatccgg aaggcgtcta cgcccggcct
    23581 gaattcctgg tccaacggtt tgaaagcaac cggagaaaaa acatctgtgg gactgaccga
    23641 cattcctccg aatgtcacag cctgccgtcc aataaaatat tcccagtttc ccgattgaca
    23701 acgcattaag gctcgatcca aattttgctc aagctgccaa agttcagaag tgtcagttgc
    23761 tgcagggctt ccggtcaggg agtgattcag atctgcaatt ctgtaaattc tgctgaaaga
    23821 gtttttgatg gagatcgtgc ttgcttgtgc tgcagaatcg atccaggcag gaaacaattc
    23881 atactctatc tcagtcgcac aattttccga caaagcaaaa tcagtttgtg cacgaaaacg
    23941 gttaaagaaa aaactggtac tatattgtgt tgaattttgg gttgcaatta aactttttgt
    24001 actgaatgaa aactctgcag aaaaaacctt cggaactaat atgttactcc aaagcagcac
    24061 ctgcagaatg agtcgaaaga agaaattaat tttttgcctt ccgcaaaaaa aactacttga
    24121 gctcatccga tacaatccgg ccgtcagtta gagttatcaa gcgtttggca cgctccatta
    24181 tttgaggatc atgtgtggaa aatacaaagg tcatctttag ttcttcattg aattttctca
    24241 tcatttccag tagttgccct ccagtttcag aatccaaatt tgcagtaggt tcatccgcaa
    24301 gaataatatc cggctttgaa accatggccc gtgcaatggc tactcgctgt tgctgacctc
    24361 cggaaagttc tgcaggaaac ctgtttccct taccttccaa acccagtgca gccaggaaag
    24421 tgtgtacccg ttctttacgt tctgcagccg gtacatgctg tagcagcatt acatattcga
    24481 tgttctcaag gactgtcaaa acaggaatca ggttataggc ctggaaaacc aggcctatat
    24541 gttctctacg aaactgggcc agttcgctgc cgctcatttc ctgaagtgag cgcccggaaa
    24601 ccttaatctc accggaactt acattatcca gaccacataa aatattgagc aaagttgttt
    24661 ttcctgaacc tgaaggtcca acaatggctg caaaatctcc tcgcttaata tccagatcca
    24721 cattcctgag agcctggatt tctgtttcac cttctccaaa tgccttaaat attttatgtg
    24781 caacaattac tgtttcttcc ataagtctgt ttctgtttaa aattttcgtt ttcgcagtgc
    24841 ttcaacgggt aacatcttcc agagtttgaa ggcaggatag agcgaggcta aaagggtaaa
    24901 tcccagactc ataaacggat ataaaaccag cctgctccat ttgtaggaag gatatatttt
    24961 ttcctgaaag gtaactccgg aaaactcaat gccggtataa tctattccat aatttgcaaa
    25021 taaagctgtc acaaaaagcg caagaatcag tccgacaatt atactgacaa accccagaca
    25081 aactgcctca aagagcatta tacgggctgc ctgccagggt gtagttccaa ttgatttgat
    25141 taccccaaat tcaaacattc gttcgtaaag ccccatcaac aacgtattgg tgatgcccag
    25201 tgcaattatc agaaataata ttgttcccat aattgccatt gaaagatcgg tcatttgcga
    25261 aatcatatag agtgagggca ttaattctgt ccagagcagt aattcgtttt tcgaagaatc
    25321 atccggtttt ttcaaagggt taaccggagt tccgtcctgt gttaattttg aataacgcaa
    25381 agctatttgg tgaatccgcc cctcaagttt gagcatggac tgcaaagttt ttcgtgctac
    25441 cagaaccatt gaagcatcca ttttctcttc gccggttttg aaaataccac tcaagcggaa
    25501 gagttcctgt accatctcac cggactctgc atctgcagca gttaagacca aacgatctcc
    25561 aagacctaac tccagttttt ccgcaagttt ccaccccaga atcagtttct gtccggaaac
    25621 tgaatccaga tattctcctt caataattgc ctcatcaaat tttgaaagca gttgttcttc
    25681 ttcaggatca actccaagtg aaaaaacagg ttctcccccg ccggatgaag aaagcatgct
    25741 ttgtgttaca atgcgcaaac tgcgcgcttt caaatcggga tgtttttcca gctgcaacag
    25801 tgcttgatcc aaatcattta tggtgagttt tacatcctgt tcatccctaa aaccaagggc
    25861 gtgcatttgg gcatgaccca tcaaggaatc tgtggcattt tttaccatgt tagtgttcat
    25921 tccaaccatc atggtatcca tgaaaatcat ggcaaccagg ccgattgcaa tcaccagagc
    25981 tgagagccag gtgcgtgtcc ggtttctcca aagattacgc caggcaagtt ttaataacat
    26041 ttaggttgtc agttttcggt aattcttagt gccttagtgc cttagtgcct tagtgcctta
    26101 gtgccttagt gccttagtgc cttagtg
//
LOCUS       DTVD01000147            1938 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_26505_length_1938_cov_319.927244, whole genome
            shotgun sequence.
ACCESSION   DTVD01000147 DTVD01000000
VERSION     DTVD01000147.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26505_length_1938_cov_319.927244"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(28..423)
                     /locus_tag="EYO68_05200"
     CDS             complement(28..423)
                     /locus_tag="EYO68_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HIC07236.1"
                     /translation="MKIKNAANSLAKLGHSTRLSIYRLLVQAGHRGLTVGEIGKRLGI
                     APTTLNHHFRVADSFKTVCEQIELFRATVLHVDRLPFKIRTISLEFLGRAVQSSEFLE
                     RMLGNGLVIAEMDEFCFFTGKPDSSPNSG"
     gene            complement(460..870)
                     /locus_tag="EYO68_05205"
     CDS             complement(460..870)
                     /locus_tag="EYO68_05205"
                     /inference="COORDINATES: protein motif:HMM:PF07884.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIC07237.1"
                     /translation="MLDASIISLIALGGAALFGLQGSISLAVRHHLVRELQQRSACDR
                     VAHSKEANIANIPVAYGAVIFYGLVLFLLFRGMNQPEIPLFWLNSAMVAALIVTCYYA
                     YVMFFRLNIVCMGCLRIYLANLMMAAALLAYQFN"
     gene            990..>1938
                     /locus_tag="EYO68_05210"
     CDS             990..>1938
                     /locus_tag="EYO68_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07238.1"
                     /translation="MKKTPAWDLLTLTVCRIDPAETRFNWFPGNLSEEDGKSLEQNGI
                     LIPLLLQAVPGKKYRIIDGFKRISWLTSNNTASDQKQQETPIPCFILPESMPEREATN
                     IRLETLSTSSGNFSGIQIGRVLKQLQDSDFTTEEIATQFLPRLGLKPSARLVRQLLNL
                     HNVLKTMTLPESLLRLGSEDLLPLLKFSQSALPDLAALSERMEVGGKKWRNLLQVLDE
                     VSRLREMSADEVLKLPEILEIIGRSSLQAPVRYRLLKQQLDTWRYPELSDLRQRFEQG
                     RQRLNLSPRITLEIDPYFENDDLTLSLKIRSVQELRKHLK"
BASE COUNT          580 a          422 c          408 g          528 t
ORIGIN      
        1 tctccaccga accattgtgt aaccttttca gccggaattg gggctactat caggctttcc
       61 agtaaaaaag caaaactcat ccatttcagc aataaccaac ccgtttccca acattctttc
      121 cagaaattcg ctactctgga ctgctctccc caaaaactcc aaactaatgg ttctgatttt
      181 aaaaggtaaa cgatccacat gaagaaccgt agcccgaaac aattcgatct gttcgcaaac
      241 tgttttgaaa gaatccgcga ccctgaagtg atgattgaga gtagttggag caattccaag
      301 ccgcttgcct atttcaccaa cagtaagacc tctgtgtcca gcctggacca gcaaacggta
      361 tattgaaagc ctggtagagt gtcctagttt tgctaaagaa tttgctgcgt ttttaatttt
      421 catctgcaag agaatgtccg gttaaaatgc ggaaaatatt cagttaaatt gataagccag
      481 cagcgctgcg gccatcatta agtttgcaag gtagatgcgc aggcagccca tacaaacaat
      541 atttaatctg aaaaacatga cgtaggcata ataacatgtc acaatcaggg ctgcaaccat
      601 agctgaattg agccagaaaa gcggtatttc gggttgattc attccgcgga atagcaggaa
      661 aagcacaaga ccgtaaaaaa ttactgctcc ataagctaca ggaatatttg caatatttgc
      721 ttctttggaa tgtgcaactc tgtcgcaggc agaccgctgt tgaagttctc gaacaagatg
      781 atgtcttaca gcaagactta tagatccctg cagtccaaag agggctgcac cgccaagtgc
      841 aattagactt attatagatg catcaagcat acttaattgt cttgttttgt tccggacttg
      901 aatctgagtc aaggtttgat gatactgaaa gtatcaaaat attagcatcg gaatgaactg
      961 caaaattcct tcctgaacaa ttacttcaaa tgaaaaaaac gcccgcctgg gacttgttaa
     1021 ctctcactgt ctgccggatt gaccccgctg agactcgttt taactggttt ccgggtaatc
     1081 taagcgaaga ggacggaaaa tcactggagc agaatggaat cctcatacct ttactgctgc
     1141 aggcggttcc gggtaaaaaa tatcgtataa ttgacggctt caagcgcatt tcctggctca
     1201 cgtccaacaa tactgcctcc gatcaaaaac aacaagaaac accaattccg tgctttattc
     1261 ttcctgaatc gatgccggag cgggaggcga caaatattcg cctggaaact ttgtccacat
     1321 cttcaggtaa tttttccgga atacaaatcg gcagagtatt aaaacagtta caggattccg
     1381 attttactac cgaagaaatt gccactcaat ttttacccag acttggcttg aaaccttcag
     1441 caagacttgt tcgtcaatta ttgaatttac acaatgtttt gaaaacaatg actctgcccg
     1501 aatctttgct gcggttgggg tctgaagatt tattgccttt gctgaaattt tcgcagtcag
     1561 cacttccgga ccttgccgca ctcagcgaaa gaatggaagt agggggaaag aaatggcgca
     1621 atttgcttca ggttttggat gaagtcagtc gtttaaggga aatgtctgca gatgaagttt
     1681 tgaaacttcc ggaaattttg gagattattg gacgttccag ccttcaagcc cctgtacggt
     1741 atcgtttgct taaacagcaa ttggatactt ggcgctatcc ggaattgagc gatttgcgtc
     1801 aaaggtttga acagggacgt caaagactga atttatctcc gcgcataaca ctggaaattg
     1861 acccgtattt tgaaaatgat gatctgactt tgtcattgaa aatccgttca gtccaggaac
     1921 tccggaaaca tttgaaag
//
LOCUS       DTVD01000148            7802 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2652_length_7802_cov_396.495159, whole genome
            shotgun sequence.
ACCESSION   DTVD01000148 DTVD01000000
VERSION     DTVD01000148.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2652_length_7802_cov_396.495159"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(2..658)
                     /locus_tag="EYO68_05215"
     CDS             complement(2..658)
                     /locus_tag="EYO68_05215"
                     /inference="COORDINATES: protein motif:HMM:PF01865.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HIC07239.1"
                     /translation="MNPLSLFFKKQYAVEEKIQRLLRYLEDMGQLYRGAYEAYLDGSY
                     DDFAQRNEDLNKIEKEMDDLGLQIQMTLMRESLMPDSRDDLLWFLTKLDKVPSSFKHS
                     LGAIAIEKPEIPQDFQDPLKKMLAHTHDAVKALAHATDSLFTDLRAVRQHVEEVGRQE
                     SEVDKVEYKLLRTVFENEKFELARQYQLKGILKQLGAVTNLAEDVADAVLILATKHSA
                     "
     gene            complement(670..2145)
                     /locus_tag="EYO68_05220"
     CDS             complement(670..2145)
                     /locus_tag="EYO68_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIC07240.1"
                     /translation="MLDPTVLIFLSSGLFLGWSLGANDAANVFGTAVGSRMVRFTTAA
                     LICGVFVVLGAFFSGTGAAQTLGKLGAVNALGGSFMAALAAGLTVLWMTKLGLPVSTS
                     QAIIGSIIGWNLFSDSYTDISSLLKILSTWIICPLLSAVIAALLFTLAKIFVRKIGVG
                     LIRMDGYTRLALILAGAFGAYSLGANNIANVMGVFVPVAPFPDLQFGQDFSVSSAQQL
                     FLVGGLAIAVGVFTYSKRVMMTVGSELMTLTPLAAWVAVMSHSIVLFLFASERLEQLL
                     ANMSLPTIPLVPVSSSQAVVGAVIGIGMLQGGREIQWPRIYGIVRGWAITPMISCLLC
                     FVGLYFLQNVFQQEVQRESNYLLSTRVLEKFQKEGIETTGLNQLSDSTFSSSAELVRA
                     VSSIVPLSSQQGLKVVEFSLQKSLLITQEKIASMDKKGLSSIQLDALNQLQGQTFNFP
                     WQLGDSLAEISSEWEVRGGGLKNKLHDRKIKQKLAYLYRNF"
     gene            complement(2370..2849)
                     /locus_tag="EYO68_05225"
     CDS             complement(2370..2849)
                     /locus_tag="EYO68_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIC07241.1"
                     /translation="MSKTKLPASKEEWFFENYKQDAVFEFGPVEVEEEEVLKFARRYD
                     PQPFHIDLEAAKKSPYKGLIASGWQTCAYVMRELVEHYFSPVSSLGSPGIDELRWILP
                     VRPGDKLMVRATILETKRSRSKPDRGIVRSFIEAVNQRQETVMSFKSVNFMLCRKKT"
     gene            complement(2873..3427)
                     /locus_tag="EYO68_05230"
     CDS             complement(2873..3427)
                     /locus_tag="EYO68_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07242.1"
                     /translation="MSFPKQIFLLFFFISGSTQVFGASFPVLPEVQVYHTVGSRGSNY
                     STGALGLSLHLYDEPTGPGRYLHLLALSIPAKTAAWKSRVGDLFLSENGTGFEIGVHD
                     QWVSPSGKGATLSLRKIQWPKVRQGDTDTGEATSDILHMGWSWISGTPIITVWLMGLD
                     IATLNLPHDRKLEMQFALGMRTAF"
     gene            3537..4928
                     /locus_tag="EYO68_05235"
     CDS             3537..4928
                     /locus_tag="EYO68_05235"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIC07243.1"
                     /translation="MRYSSTRGQDKNLLFEEAVMMGLADDGGLLVPDEFPQVKSKFHE
                     WRALSFSELSLEIMLLFTSGRIPREEFAPLVQRSYTNFRHPKITPVKSVGKIHILELF
                     HGPTFAFKDVALQFLGNLFECFLAKRDHPLRIIGATSGDTGSAAIHGMRGKKGVDVFM
                     LHPKGRVSPIQELQMTTVLDSNIHNLAIEGSFDDAQAIVKALFNDQEFKHSYHLGSVN
                     SINWARILAQIVYYFYAWFQVTRDNSEFVSFAVPTGNFGNVLAGYYARRMGLPIDKLV
                     VGTNENDILHRFFCNGEYKQTEVKETLSPSMDIQISSNFERYLFELAGRSSEQLQSWM
                     DDFESNGKLAFGNDLLKKAQNDFVSALVNDEEILEMVSRFNQENGYLLDPHSAVGVRA
                     AEKNDIQTPVICLACAHPAKFGDAIRKALGSEPELPDELSQLTNLETRIKTVAADPET
                     IKQVVLETLEARS"
     gene            4971..6032
                     /locus_tag="EYO68_05240"
     CDS             4971..6032
                     /locus_tag="EYO68_05240"
                     /inference="COORDINATES: protein motif:HMM:PF03279.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07244.1"
                     /translation="MLSSDSLETESKILEPTYDEFKKNRPAWTTSLRRGVEYAVFRFG
                     LYLGRRLSLKQLQKLGRVIGSLAFLVLRKDRGIVEKQLELIFPEIDAAQRRQWTKECF
                     GQFGQMLFEFLSLPQILHDEANLLQVENEEALTNAIHAEKGVILLAMHTGNWELISAY
                     ARRTGLKMKAATTNFPDPRINKLIESQRENGNMEILRRGTSAAIRKLLGCLRNKEVLV
                     LAIDQDTNVLSTWVPFFGIPAKTPVGAAVFALKTGAAVVSYNVIRQTDGTFKMRFETL
                     GTFVRQHHEMEQDVYAVTRKMNLHLEQRIRENPQQWAWFHRRWRHRPSEDERQKMEEL
                     EQNEIQFLERLDSGKHIKA"
     gene            6063..6839
                     /locus_tag="EYO68_05245"
     CDS             6063..6839
                     /locus_tag="EYO68_05245"
                     /inference="COORDINATES: protein motif:HMM:PF00561.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07245.1"
                     /translation="MSIVVSNKQKIHYELAGDKGPWMLLHPPHLIPMSAWKEEGYVRQ
                     LEKDFRLVMIEPLGQGLSDAPEDSSHYTISSRIRHILDIMREVQADFAFFFGVGLGAQ
                     VGFQMSKEFPRRIRSLISVGAQPYQELEEAKYLAEKLDKLRSGNVSAYLQQWHSLDHL
                     SEEQEKLILEGNSEAYALGLEASINWEGLGDDLKSLGTTTLLFTAKAEPRFLSVRDAG
                     KRLRNGRYVILPKVTHSHGLWSSDLIIKPLLEFTRRERSN"
     gene            6854..7774
                     /locus_tag="EYO68_05250"
     CDS             6854..7774
                     /locus_tag="EYO68_05250"
                     /inference="COORDINATES: protein motif:HMM:PF04773.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07246.1"
                     /translation="MQSIRRCVLTISFFLLSSTVPVLSPAKTVPALAVLSRIKAKVKA
                     GYSKKMTEGFNGQMLLRRYRVRTEKKGRATIFFNDGSEVRLFGETELTIGAKKSRNYR
                     WVRYRLFLHTGSFWGHFVRGKNPVEIGGGGMRLQLSNASLRFSREKTGIDIAVPSGMA
                     KVSNKSSSVKLLAGQRLYQVRKNDFLPQKISLIPNQLKLWIEPSAPVFKGKESLKLNL
                     HFQIVRYGTDRPVDRPGSVYLKSNYYNLKIPDSIRLNKDGKAKTTIEVAPPRSNDRTF
                     EGTVTLHSIMDQSGYNDVQDGLLKVRFTSH"
BASE COUNT         2302 a         1721 c         1736 g         2043 t
ORIGIN      
        1 ttcaagccga gtgcttggtg gccagaataa gtactgcatc cgcaacgtct tcagcaagat
       61 tggtgactgc acccaactgc ttcaaaatgc ctttcagttg atactgccgt gccagttcaa
      121 atttttcatt ctcaaaaact gttctcagca gtttatactc taccttgtcg acttcactct
      181 cctgacgccc aacctcttcc acatgctgtc ggacagccct caaatctgta aatagtgaat
      241 ctgtggcatg agccagagct tttactgcat catgggtgtg agccagcatt tttttcagtg
      301 gatcctgaaa gtcttgaggg atctcaggtt tttctatagc tatagcgccc agtgaatgct
      361 tgaagctact ggggacttta tccagcttgg tcaaaaacca gagtaggtca tccctggaat
      421 ccggcatcaa agattctcgc atcagtgtca tttgaatctg aagtcccaga tcgtccattt
      481 ctttttcaat tttgttgaga tcttcattgc gttgagcaaa atcgtcatag cttccatcca
      541 gatatgcctc ataagctccc cgataaagtt gccccatatc ttccaggtaa cgcaaaagtc
      601 gctgtatttt ttcttctaca gcatactgtt ttttaaaaaa caaacttaaa ggattcatct
      661 ttctctccgt taaaaatttc gatataaata agctaatttt tgtttaattt tgcggtcatg
      721 cagtttgttc ttcagcccac ctccgcggac ctcccattct gaactgattt ccgccagaga
      781 atctcctaat tgccagggga aattaaaggt ttgaccctgc agttggttca aggcatccag
      841 ttgaattgaa gagagacctt ttttatccat ggaagcaatt ttttcctgag tgatgagcag
      901 acttttctgt aacgaaaact ctaccacttt aaggccctgc tgactggaca aaggtactat
      961 agaacttact gcccttacca gctcagcaga actactgaat gttgaatctg acagttgatt
     1021 caaaccggtt gtctcaattc cttctttttg aaatttttct agaacacgag ttgacagcaa
     1081 atagtttgat tcacgctgta cttcttgttg gaaaacgttt tgcagaaaat acaatccgac
     1141 aaaacaaagt aaacaggaaa tcataggcgt gatggcccag cctctgacta taccgtaaat
     1201 tcgcggccac tgaatttctc ttccaccctg caacattcca attccaatca ccgctccaac
     1261 cactgcttgg gaacttgaaa caggaaccag tggaatggta ggtagcgaca tgtttgccag
     1321 cagttgttcc aggcgctcag aagcaaaaag gaagaggaca atcgagtgtg acattactgc
     1381 tacccaggct gccagtggag tgagcgtcat cagttctgag ccgactgtca tcataacccg
     1441 cttggaatat gtgaagacgc ctaccgcaat agccagaccc cccactaaaa atagttgctg
     1501 tgcggaagaa acactaaaat cttgaccaaa ctgcaaatcg ggaaacggag ccacaggcac
     1561 aaaaactccc atcacgttgg caatgttatt tgctcccaga ctgtaggcac caaaagctcc
     1621 ggcaaggatc aaagcaagcc tggtatagcc atccatacgg atcagcccaa caccaatttt
     1681 tcgtacaaaa atctttgcca atgtaaacaa aagagcagca atgactgcgg aaagcaaagg
     1741 acaaatgatc caggtcgaga gaatcttcag taaagaagaa atatctgtgt aagaatcact
     1801 gaacaagttc cagccgatta ttgaaccaat gatggcctgg ctggttgaga cagggagtcc
     1861 gagttttgtc atccaaagaa ctgtcaatcc tgcagccaac gcagccatga atgagccccc
     1921 aagcgcattt accgcaccaa gtttacctaa tgtctgcgct gcaccagttc cgctgaaaaa
     1981 ggcacccaga acaacaaata caccgcaaat cagtgccgca gttgtaaatc gaaccatacg
     2041 gctgccaacc gcagtgccga aaacgttggc tgcatcgttt gcaccaagtg accagcccag
     2101 gaaaaggccg ctgcttaaaa atattaggac tgtaggatca agcaaaacta tactttttga
     2161 aaattatttt taaaatatca gggtgttatt agatcactct tcgaaggcaa ctgcaaggtc
     2221 tatctggacc ccagccagtc caagaaggtc gatcaaaaac aaaatttttg cgaaagcagc
     2281 cgcaaaaaat cagcctcaga attattgtga atgtacggat gacctgctca gaaaataaag
     2341 gaggaggatg gattgtatct gaagatgtcc taggtttttt tacggcaaag cataaaattc
     2401 acagatttga agctcattac agtttcctgc cgctgattaa ctgcctcaat aaatgagcgg
     2461 acaattcctc tgtcgggctt tgagcgagag cgttttgttt ctaaaatggt ggctcggacc
     2521 atgagtttgt ctcccggacg caccggtaaa atccagcgca gttcatcaat ccccggagag
     2581 cccagactgg aaaccggtga gaagtaatgc tcaaccaact cccgcattac ataggcacag
     2641 gtctgccagc cactcgcgat cagtcctttg taaggacttt tttttgcggc ttctaggtct
     2701 atgtgaaaag gttgaggatc atagcgtcta gcgaatttga gtacctcttc ttcttctacc
     2761 tcaacaggtc caaattcaaa gactgcgtcc tgtttataat tctcaaaaaa ccactcctcc
     2821 tttgaagctg gcaacttggt cttagacatc aaactcctaa atgtgataat aatcaaaaag
     2881 ccgtccgcat tcccagtgca aactgcattt ccagtttccg gtcatgtgga agattcagtg
     2941 tcgcaatatc cagtcccata agccacaccg taataatggg agtccctgag atccaactcc
     3001 atcccatgtg aagaatgtca gaagttgctt cccctgtatc agtgtctccc tgacgaacct
     3061 tgggccactg gattttccgc aaagataagg ttgccccttt cccggatgga gacacccatt
     3121 gatcatgtac cccaatttca aaaccggtcc cgttttcact caaaaagaga tctccaaccc
     3181 tgcttttcca ggcagcagtt tttgcgggaa tgcttaaagc cagcaagtgc agatagcgtc
     3241 cgggaccggt aggttcatca taaagatgaa gtgacagtcc tagtgctccg gtgctgtaat
     3301 tgcttcctct acttccaaca gtatgataga cttgaacctc cggaagtact gggaatgatg
     3361 ccccaaaaac ctgggtgctg ccagaaatga aaaaaaagag caaaaatatt tgttttggga
     3421 atgacatcgt ccaattgact tggagatttg ctaaccaata cgaaaaacca ctgtcattca
     3481 tccgaaatga atggtgacag aaaaccgttt cagaagtaca aacatacaac aaaataatgc
     3541 gttattcaag tacacgtggg caagacaaaa accttctttt tgaggaggca gtaatgatgg
     3601 ggcttgcaga cgacggcggt ttgctggttc cggatgaatt tccgcaagta aagagcaagt
     3661 tccacgaatg gagagcatta agttttagtg aattgagtct ggaaattatg ctgctcttta
     3721 ccagtgggcg aattccacgg gaagaattcg cacctttggt gcagagaagc tataccaact
     3781 ttcgccaccc taaaataact ccggttaaat cagttggaaa gattcatatt ttagaattgt
     3841 ttcatggacc gacctttgcg ttcaaagacg tggccctgca gtttttaggc aacctttttg
     3901 agtgtttcct ggcaaagcgt gaccatcctt tgagaattat aggggccacc tcaggagaca
     3961 ccggaagtgc cgccatccac ggaatgcgtg ggaaaaaagg cgtggatgta ttcatgcttc
     4021 atccaaaagg acgggtcagc ccaattcagg aattgcaaat gacaacagtt cttgactcaa
     4081 acatccacaa tctggccatc gaaggttcat ttgatgacgc gcaggcgatt gtgaaagcac
     4141 tttttaatga tcaggaattc aagcacagtt atcatttggg ttcagtcaac tccatcaact
     4201 gggctcgaat tctggcacaa attgtatatt atttttacgc ctggtttcaa gtgaccagag
     4261 ataattccga gtttgttagt tttgcagtac caactggaaa ttttggcaat gtgctggccg
     4321 gatattatgc caggcgcatg ggcctgccaa tcgataagct tgttgtagga acaaatgaaa
     4381 atgacattct gcaccgcttt ttctgcaatg gtgaatacaa acaaactgaa gttaaggaaa
     4441 cattaagtcc ctcaatggac attcaaattt ccagcaattt tgaacgttat cttttcgagc
     4501 ttgcaggaag atcctctgaa caactgcagt cctggatgga tgattttgaa agtaacggta
     4561 aattggcgtt tgggaacgat ctcctcaaaa aagctcaaaa cgactttgta tctgcacttg
     4621 taaacgacga ggaaattttg gaaatggtga gccggtttaa tcaggaaaat ggatatttac
     4681 tggacccaca ttctgcagtt ggtgttcgtg cggcagaaaa aaatgatatc cagacgcccg
     4741 tcatttgttt agcctgcgca catcctgcca agtttggaga cgcaatccgg aaggccctgg
     4801 gttccgaacc ggaattaccg gatgaattat ctcaacttac aaacctggaa acgcgcatta
     4861 aaacggttgc ggctgatccg gaaacgataa agcaagtggt gctcgaaacg ctcgaagccc
     4921 ggagttaaca gccttagcca tgataactga aacagacaga cgatagctaa attttgagtt
     4981 ctgacagtct agaaacagaa tctaaaatcc ttgagccgac atacgatgaa tttaaaaaaa
     5041 atcgtccggc ttggacaact tcgctgagac gtggagttga gtacgcagtg ttccgttttg
     5101 gattgtattt ggggagaaga ctgtctctaa aacaattaca aaaattggga cgcgtaatcg
     5161 gaagtttagc ctttctggtg ctcagaaaag atcggggaat tgtcgaaaaa cagcttgaac
     5221 tgatttttcc ggaaatagac gcggctcagc gtagacaatg gacgaaggag tgttttggtc
     5281 aatttggaca aatgcttttc gagttcttga gcttaccgca gattttacac gatgaagcta
     5341 atttgcttca ggttgaaaat gaggaagcgc tgacaaatgc aattcacgcg gaaaaaggtg
     5401 ttattctatt ggccatgcac acagggaact gggaattgat ttctgcatat gcacgacgca
     5461 caggcctgaa gatgaaagcc gcaacaacga attttcctga tccgcgaatt aacaaactta
     5521 ttgagagcca gcgtgaaaat ggaaatatgg aaattctacg tcgcggaaca tcggccgcaa
     5581 tcagaaaact actaggctgc ctccggaaca aagaagtgct ggttttagcc attgaccagg
     5641 atacaaatgt actaagtaca tgggttccgt tttttgggat tccggcaaaa actcctgttg
     5701 gtgctgcagt ctttgccctg aaaaccggtg cagctgttgt gagttataat gtaattcgcc
     5761 agacagatgg aactttcaaa atgagatttg aaacactggg cacttttgtc cgtcaacatc
     5821 atgaaatgga acaggatgtg tatgcagtta cgagaaaaat gaatctgcat ctggaacagc
     5881 gtatacgtga aaatccgcag caatgggctt ggttccaccg ccgatggaga catcgtcctt
     5941 ctgaagacga acggcagaaa atggaagaat tagagcaaaa cgaaatacag ttcctggaga
     6001 gacttgactc agggaaacac attaaagcct gaaacttaaa acctgaataa tttagctgaa
     6061 atatgtcaat tgttgtttca aataaacaaa agattcatta tgaacttgct ggagataaag
     6121 gtccttggat gttgctgcat ccgcctcatt tgatccccat gagtgcctgg aaggaagaag
     6181 gatatgtgag gcagttggaa aaggattttc ggctggtaat gattgaacct ctcgggcaag
     6241 gtttaagcga tgcccccgag gattcttcgc actacaccat ttcttccagg atcaggcaca
     6301 tactggacat tatgcgtgaa gtacaggccg attttgcatt tttttttggt gtcggtttgg
     6361 gtgctcaggt tggatttcaa atgtccaaag aatttccccg acgaattcgc tcattgattt
     6421 ctgtaggtgc acagccttac caggaactgg aagaagcaaa atatctggca gaaaaactgg
     6481 ataaactgcg ctcagggaat gtgagtgcct atcttcagca atggcactct ttagatcatt
     6541 tatctgaaga acaggaaaag cttattctgg aaggcaattc ggaagcttac gcactcggtc
     6601 tggaagcatc cattaactgg gaaggtttgg gtgatgattt gaaatcactg ggaaccacta
     6661 ctttactttt taccgccaag gcagaaccac gatttttatc tgtacgggat gccggtaagc
     6721 gtttgcgcaa cggacgttat gtgatccttc caaaagttac acatagccac ggactgtgga
     6781 gttccgattt aattataaaa cctctgctgg aattcacccg tcgggaacgc tcaaactgaa
     6841 tcttcgcaat aacatgcaat ccatccgccg gtgtgtttta acgatcagtt tttttctgct
     6901 gagcagtacc gttccggttc tttctccggc aaaaactgtt ccggctttag ctgtactttc
     6961 ccggataaaa gctaaagtga aagccgggta ctccaaaaaa atgactgagg gtttcaacgg
     7021 acaaatgttg ctgcgccgtt acagggtccg gacagaaaaa aaaggaaggg caactatatt
     7081 ttttaatgat ggttccgaag tccgattgtt tggtgagaca gaactaacga tcggcgcaaa
     7141 aaaaagccgg aactaccgtt gggtgcgtta tcgactgttc ttgcatacag gtagtttttg
     7201 gggacatttt gtgcgcggaa aaaatcctgt ggaaatcggc ggtggaggta tgcggttgca
     7261 gctttcaaat gcctctctga gattttcaag ggaaaaaacc ggaattgaca ttgctgtacc
     7321 ctccggaatg gccaaggttt ctaacaagag ttcctccgtt aaattacttg caggtcaacg
     7381 actctatcaa gtccggaaaa acgacttttt accacaaaaa attagtctga ttccgaatca
     7441 gctgaaactc tggattgaac cttcagcacc ggtttttaag ggaaaggaat ccctaaaact
     7501 aaatctgcat tttcagatag tccgatacgg cacagatcgt cctgtagatc gtcccggttc
     7561 ggtatattta aaaagtaact actacaattt gaagatacct gattccatcc ggctcaacaa
     7621 ggacggcaag gcaaagacca ccattgaagt tgcaccgccc cgttcaaacg accgtacatt
     7681 tgagggtact gttaccttac attcaataat ggatcaaagc ggctataatg atgttcagga
     7741 tggattgcta aaagtaagat tcacaagcca ctgaaaaatg atggcaacag agagtacttt
     7801 aa
//
LOCUS       DTVD01000149           25798 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_266_length_25798_cov_280.589209, whole genome
            shotgun sequence.
ACCESSION   DTVD01000149 DTVD01000000
VERSION     DTVD01000149.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 25798)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25798)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25798
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_25798_cov_280.589209"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            73..303
                     /locus_tag="EYO68_05255"
     CDS             73..303
                     /locus_tag="EYO68_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIC07247.1"
                     /translation="MSDFDTELNCEGLNCPLPVLKTKKKIDGMSSGEILKMTSTDPGS
                     VNDVTFWSKINKNYLLSHAEDGGVYTFYIQKK"
     gene            567..1607
                     /locus_tag="EYO68_05260"
     CDS             567..1607
                     /locus_tag="EYO68_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC07248.1"
                     /translation="MSTQPYIGFRQLFDQDTSTFTYLLWDLDTREGVIIDPVREQFER
                     DLCLVGELGVRMVYALDTHVHADHVTSLGMLREEMRLQTAVGEPSGVPCADIMLNDGY
                     TLRFGRHTLTALATPGHTDACTSFKVENMVFTGDALLIRGCGRTDFQQGDPEMLYRSI
                     TQKLYALPDETLVYPGHDYNGKSVSTIGEEKQYNPRIPATQTESDFAELMNSLNLPRP
                     KHIDEAVPANMGCGISVDHGHLTEEVFGVRDLQKILTALSEDEVVIDCRTPDEYEAGH
                     IPGAINLPMGKELDQLGELRDYRKIYLYCYSGRRSQTVFATLTSKGLDNLVCLGSSGM
                     AEWKKFGYPVER"
     gene            1641..2063
                     /locus_tag="EYO68_05265"
     CDS             1641..2063
                     /locus_tag="EYO68_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIC07249.1"
                     /translation="MTEFEVAMPLAGGILIGIAAAMMMLFSGKIAGISGIFGGMLFQQ
                     GEERTWRSAFVAGLIAGGVLLYSINTEYFENSSGRGLVAVTIAGLLVGIGTRVGGGCT
                     SGHGVCGIGRLSARSLAATVTFMVAGMAMVALVQPLYH"
     gene            2072..2518
                     /locus_tag="EYO68_05270"
     CDS             2072..2518
                     /locus_tag="EYO68_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIC07250.1"
                     /translation="MAKSNIAAFLCGLLFSIGLGISGMTQPQKVIGFLDVFGEWDLSL
                     AFVMGGAVISYLLLQLWIQLRFSKPMLGETFQIPSRKDLDRSLIFGALLFGVGWGLGG
                     YCPGPAITSLGSGSVNAVLFVVAMGMGMLLADRVISPILTHQALKS"
     gene            2650..2811
                     /locus_tag="EYO68_05275"
     CDS             2650..2811
                     /locus_tag="EYO68_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC07251.1"
                     /translation="MAFTVKLLINGESVDGDGDSLAVQNPSTGSTICEVAEATAEQVE
                     ATVRAARDA"
     gene            2804..3973
                     /locus_tag="EYO68_05280"
     CDS             2804..3973
                     /locus_tag="EYO68_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIC07252.1"
                     /translation="MLDIFYCLNIQKKSTTSSFDNLLKNPEKTITSIIKRVATGPELS
                     KSISYKESRASMRLVLEGLADPVQAAMFLIGLRMMRETDDEYKRILQDIRNKTKTVVA
                     DVDELVDIADPYNGYGRSLPSSPFLPVLLAESGVPAVSHGVETVGPKFGVTHHQILQA
                     AGVSVELTGEEAGKQISNPEIGWAYVDQSQFCPSLFGMAEFRKKIVKRAAISTAEVLT
                     GPVRGRNKTHLLTGYVHNEYPPIYTMLARHSGFNSALLMRGTEGGVTPSLRKRGEFVR
                     YWEQSEDVVVEADPSKIGIEQENRLVPIAHALMPIIDREFGPDENNAEIAKLAAKEGL
                     AAIEGKKSATCDALLYSASLTLWHLGRYDSVPDAASMVREILFSGKAAARFRRAA"
     gene            4415..5605
                     /locus_tag="EYO68_05285"
     CDS             4415..5605
                     /locus_tag="EYO68_05285"
                     /inference="COORDINATES: protein motif:HMM:PF04143.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07253.1"
                     /translation="MNTIETIKEPENLKEVFASGYAKMFGKQWPMWVGGLLFGALNVF
                     MFAYEKAWSVSDGVRNWGNWFFNTIEVSEMIIIEPHLFTTSILNFGIIVGAMASALLS
                     KQFQVRGAPARELIKALIGGILMGIGSVLSFGCNIGGFFSAISALSLAGVAMMAGLMI
                     GAFIGLKLLVWEITYLSPASSHASRKISGDRKKNQPLAGIIVLLLGVSLAFVYDDLDY
                     SIRGGFLVFGLVIGLLMQRTRFCFVRAFREPFLSGDGNAAKAVALAVIISVVGFSVLK
                     WSDMKDWEEIVYAGFWFGSLVGGIIFGIGMSLAGGCASGSLWRAGEGHIKLWVALIAF
                     ALSGSYFREWLVESGWIMKLGEPLFIPDLIGWKMGILSICILMLLWYLIVSWNEATRK
                     LVVV"
     gene            5960..7042
                     /locus_tag="EYO68_05290"
     CDS             5960..7042
                     /locus_tag="EYO68_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIC07254.1"
                     /translation="MDNQGEPTVSVVDYHKGETIKKIQMGETGNHHIFVIPGARYAFS
                     SQRYEKDLIWVIDLTTHEVVDTFHLSMGGKKVIAPLHIGFAYTQPLAVVGNILDKKNG
                     YLSFINTATRKPSHIVELGCHGARDAMFTLDDSKVFTTCQQEPKGVSIVDVKSRKEIK
                     VIPIKGGRAGGMTPDGKYFMVGASKAIVFIDTATNEIAKTIKVPGGGGNFTCLPDGSK
                     CYGGLRKDNSVAVIDMAKLELIKVIKTGPNANRLYLNSANPRYGLFTNESGKSEQVSV
                     IDTQDDVKIKDIITGLGPHNVAFDPTGKFALVTTKKENVATLIDTSSANPADWDVITT
                     DIAAGIQNNGVRWVPSPGAIKAAMAN"
     gene            7143..7610
                     /locus_tag="EYO68_05295"
     CDS             7143..7610
                     /locus_tag="EYO68_05295"
                     /inference="COORDINATES: protein motif:HMM:PF13501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07255.1"
                     /translation="MVIKEDFSRRKFIRYSVIGGAVVPLLSGVPYENEVYGGVFNAPK
                     DLENLTEIERIHLPKVIMPPVVEDGTQASIVVSLDHPMEEDHYIKSIQILNFADPVVI
                     KGKFYMTPINGEAFISTQIRLAGGEGTVWVVAECNKHGKWAANRTVNVAAGGC"
     gene            7558..9231
                     /locus_tag="EYO68_05300"
     CDS             7558..9231
                     /locus_tag="EYO68_05300"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00149.26,HMM:PF02872.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07256.1"
                     /translation="MENGRQTEQSMSLLEDVEVVLKFRKMTKTICFCFLTLIFFGPVF
                     ISETASVFASETEQIRIISTNDIHSYLRPIYYRYLDEFKPWGAQSREGDYVNKAEIEG
                     KIGGMAHVATMIKKLRAEKPGKNLLLDAGDTWHGAGLSLFDKGVSMVKIMNAIGYDVM
                     TPGNWEFLYPKDHFLDLVDQANFPVIAYNLTDREWEEPVLDQYVVKKVGNLKVALIGL
                     TYPWTALTSAITGAAQWFKFGIKEEEARELIEDIRKQENPDLVVFVSHGGYGLDQKFA
                     QRVDGIDIYLSGHTHDEVFDPVVWNDTIIFQGGAHGKYVVSLDVDVKDKKIQNFDYRL
                     LKVRQNQIEPDPEIEKLIEEAYEPYKKQLSEVVGQTKVMLHRRDYFQSSMGNLLTDAM
                     RDSQKTDIAFFPAWRYGATMMPGKITAEDVYNIVPTDGHVFTYSMGGKGLKTLLENIL
                     GSVVNKDPYTRVGGDMIRFSGMKLVADLAKSPGNRIVSLTVGGKPFSKDAVYSVASAH
                     TRFQNGPLFGASKVKDTGIVFVEELINYIRKNSPITSALDDRINILGFQ"
     gene            9272..9625
                     /locus_tag="EYO68_05305"
     CDS             9272..9625
                     /locus_tag="EYO68_05305"
                     /inference="COORDINATES: protein motif:HMM:PF08770.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07257.1"
                     /translation="MAKTIRDPRIRISRRIKKDKVFKVKVKFDHPSFTGLGIVDDETK
                     PVFSRAIPVSFIRNMFVYFGDQLVSRFRMSSAIADDPLFTFKLKAIQEAPVKVVFVDN
                     QGKKWEASKKIKFSN"
     gene            9701..10405
                     /locus_tag="EYO68_05310"
     CDS             9701..10405
                     /locus_tag="EYO68_05310"
                     /inference="COORDINATES: protein motif:HMM:PF00034.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIC07258.1"
                     /translation="MKKIFRFIILSFFFLIMGSHAVFAGTIEEEFKIEGLISPASPKA
                     LKSALEKELEVKVIDLNLKNTKTGWPVLTVQFDSGAVSREKIENVIGEIEDPAGHKYK
                     VHHGPLLANAPILDEEEEAIAILGDTAEVFPNMKNPITDKAASASRGGKLFVNNCAKC
                     HGLSGNGYGTVALSFTTWPRQLWAWNNTGPETDGYLFWFITNGRSDMPPFGLILSENN
                     RWDLINYIKTIKNPGE"
     assembly_gap    10582..10681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10726..11148
                     /locus_tag="EYO68_05315"
     CDS             10726..11148
                     /locus_tag="EYO68_05315"
                     /inference="COORDINATES: protein motif:HMM:PF03625.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIC07259.1"
                     /translation="MEVAQDIVKKVFMGKNFKDLLDDLIKEIEVRNYLIKRISNIDNI
                     HKRYSQGIAPDIDFQHYKIVEFCNLESCSGLISSNFLAGVFMPVRFVVFQRFEQEEIN
                     IAFLKPTSFARLFNSNKMMNFAKSLEQDMNYVLEEIVF"
     gene            11123..11737
                     /locus_tag="EYO68_05320"
     CDS             11123..11737
                     /locus_tag="EYO68_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIC07260.1"
                     /translation="MCWKKLFFKSKIFLLVFVPLFFLSEYIFILTAHEKKVSGTNNFH
                     GGDFTLKSTEGPFSLKDLRGSVVLLFFGYTSCPSVCPISLATISNVFSQMSPDELKRT
                     KALFISLDPEKDNLEILKQYTDYFHPNIVGLTDDIKTLTKVAKQYGVKYKKTLVPDSA
                     LGYVISHSDDIYVVGPNGKPQKTFPHDTNTVPLLAQIKRLLVVN"
     gene            11789..13372
                     /locus_tag="EYO68_05325"
     CDS             11789..13372
                     /locus_tag="EYO68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIC07261.1"
                     /translation="MNSFIKFSLCLLVFLQLNATSFAFEHEDSDVHFLEYDPNVIQKN
                     RFKKKPYFLLFAAQWCHWCHVFNEKTLTDEKVISYLNENFVNVFIDADINTSAYQKYK
                     AKGVPFTVFLNPDTSEYYKYSGTLYAEPFLEVIQDVIQNVKQGKTVDGEKIFAFEYNP
                     PTKFNKSTLDNMRNTYIKGVLDNFDTEEYGVGNGIKTILPETFLYLIKSTKGENRQDS
                     VLWISETLKKAIENIYDPIEGGFFRFAETGDWQVPHYEKMAGLNAGTVLLLYKVNEEN
                     PNPKFIEVALQSIQYLSNTLYDEETGSFLSFQAADTSYYFLNENRRKNVKPPLVISKV
                     FTDHLAVTLTYFLDVLDYSKDNNLENKVIHSLDFLSGMILNNKRIYHFYTIKEKQWRG
                     KSGLQDHVLLAKLFQRAADKFQNESYNKAYSKMLRFLKLNYYDEKKQIFVDPELDTND
                     YEYLMAMNGDIVFALMGQSKKSAIPQLSPVKPIITYFSGLDEFLEDKFWDSKSWDFLE
                     RSASFLSSADSFLATQSNP"
     gene            13543..13923
                     /gene="bamE"
                     /locus_tag="EYO68_05330"
     CDS             13543..13923
                     /gene="bamE"
                     /locus_tag="EYO68_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIC07262.1"
                     /translation="MSVGKYMNIGNHFESTERGFVIRNFAIILILLAGISLFTSGCVT
                     LGKDFPEANVSVIKIGQTTKNEIRKLFGSPWLSGIQDGELAWTYGNYDYSLFGKREAK
                     DLVIQFDEKGVVTTYTFSTTDHDE"
     gene            14020..14469
                     /locus_tag="EYO68_05335"
     CDS             14020..14469
                     /locus_tag="EYO68_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein"
                     /protein_id="HIC07263.1"
                     /translation="MNGDTLAKILLVLITLCFATFASAAEKVPFGSEPLAPEEAFVMD
                     HIIVGPSEAVIRWQIPKFYYLYKEKFIFTSKDFIIENVQFPPPEVIDDPFFGSSEIYH
                     NVVEATLHLTPTIKGESKGILEIGFQGCWEGGVCYPPVKTSLKLSGL"
     gene            complement(14625..21686)
                     /locus_tag="EYO68_05340"
     CDS             complement(14625..21686)
                     /locus_tag="EYO68_05340"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02801.20,HMM:PF07977.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HIC07264.1"
                     /translation="MQPIAVIGLSCLFPEAKTPEDYWKNLLQEKDSCTSAAAADMDAD
                     PSRFFAEKKGTPDKYYCARGGYINDFKMDPDGYLLSAETIEKLGATFQWPLHVAREAL
                     RDSGYIENTKILKECGIVLGNLSFPTKASNHILLPLYQKVIEPHLQKVLNRPDFNLGS
                     FAAETDNAQLNSHISGLPAAVVSQALGLSARSLVLDAACASSLYAVALACHYLHSGKT
                     DLMLAGAVSAADPFFVNMGFSIFQAFPESPQTFNPLDNNSGGLLASQGAGMFVLKRLC
                     DAERDADKIHAVISGIGLSNDGRGQFVLSPNSKGQVLAYQRAYESSKVPPEKIDYIEC
                     HATGTPLGDKVELDSMEQFFASPDLPEVKKIKPLIGSAKSNLGHMLTAAGMGGMIKVI
                     LALQHGEIPATRGVKAAMTSKNGGVSEKQIVQKTAVWPHNRKDRRAAVSAFGFGGTNA
                     HLIFESGVKASKRIDFPKTQNSQIMAIVGMEAIFGGCKGLHEFYQTIYDNKQHFRSLP
                     PERWKGFEQHPELKNHPQGAWLESFEMDFLRFKLQPNPKERLIPQQLLTLEVTDRAIK
                     KTKLGEGQNVAVLVAMETELEIHRFRGRVNLSTQIQDSLQAAGIVLSDEERKDLINLS
                     RNSLLEEVPINRFTSFIGNIMATRISSLWDFSGPAMTISAEENSVLRALEIAQILLLE
                     DNVDAVVVTAVDLAGSPEQVLLRQRNLPLNSGEATLSFDKNSNGWLIGEGAGTVILKS
                     VESAKKDDEQIFATVDSIAFSTGLTPESVENAANQALTEANVSAGEIGMLEVFGSGNT
                     VEDSAEMSGLAQAYAKVDAGCALGSIKSNVGHTFAASGMASLIKAALCLHHRFIPGVP
                     EWKSPKTELQSAKQFYVPVESRPWLIQSGIKQRHAAVSSLGIDNICSHLILSEASPEL
                     RQTIKVAESGDLNLFLVTGQEKSAISKGLLELEKDLLSGKEPASLAYQYYVRSNNNEE
                     KFAAVLIGSSRDELQKEIDAAKSGIETSFTGSGDWNSPRGSYFTASPLSREGKVAFTY
                     PGGFSAYVDCGRSLFQMFPGLHELDEQYLNQTGPADKRRGSNYLGELLQERRLYPRTL
                     ERLTDAEVTELQKEFIHTPIAMFESGVSSAVLNTHVMRKGFGLEPDIAFGYSMGEISM
                     LYGLGVWESMSNMSDILNRSKLFEERLAGPMNAVREAWDLGPDEFRQKPLWGCYSLQL
                     SPQIVQQEVENEAHVYLILINTPEEVVIAGEPTACERVIQQLGRPVHPIPVTDVVHCE
                     PVKTEYEEIKRVHTDKVVKCPDIDFYSAIDYEVSKLDSKTLAHNVATIYGKTVDYSRL
                     IEKVYADGARIFVDLGPRTTCSKWISKTLDKRPHLSISVNRKGTDNREMILQALSSLI
                     SHRVPVKLEALFPSQPVQAEKQLMQTIKLGGTPVQQVFEKGADKLFKGTKALRHQGTE
                     FQSFKVSEFQSGSASGRAYSNLVAPDYAPGNVSVSSFSPFPGEYGSHRNSAHSAFLNV
                     RQHGMRQLAELISSNTNTKGISVSNTFQHTVSQKTTPETQQPVPQTKPCIFDYHDLLE
                     LAGGSIANVFGAEYAEIDSYRRCVRLPMEPYLLVSRVTQLDAKLGEFKPSTITTEYDI
                     PDNAWYTTDGQIPWAVAVESGQCDLMLISYLGIDFDCKGEQVYRLLDCTLTYLDDMPL
                     EGQTLRYEISINSFAKHDQNLLFFFNYECFVGNKMVLKMDNGCAGFFSDEDLSQGKGV
                     IHTAQELELKEKADKIKFPPLLHCTKTTFTRQELLEISAGNPAGCFGPEYNQQGKNPS
                     LKNAPDQFLMSDRIMSVEPHGGPYGLGYIVAEKDLAPDDWYFPCHFKDDPVMAGSLMA
                     EGCVQLLQFFLLHLGLQTLVEDATFQPIHGLPQIVRCRGQVIPGDPKMTYRVEIKEIG
                     LEPHPYAIADIDILVGDRIVVDFRDLGVQLAEKTGSTGGGNLLRVSKNQRKAYSGGEI
                     KTPDSNIQVPETSDRQLAADEQMIWEFALGDVEKCFGSEFAIYKGRSMQRNPNGDLQL
                     ISRVYDLHGKRMEFDKTMTLVSEYDVPEDAWFFRNNSYPENMPYSVLMEIALQPCGFI
                     STHSGAILTYPELDLYYRNLDGNGTLLRNPDLRGKTVLNEVKLLTTVASGNTIIQTHR
                     FSLSCDGQIFFEGDTMFGYFTGESLAAQVGLDGGKKAVPWIDETPSVSSILLDLNSVD
                     FRKALGEKPEHPYFRLCSGQLSFSDIIRIVPEGGKFSKGYVYARKEVNPEDWFFPCHF
                     HGDPVMPGSLGVEAIIQALQAFALQQGIGNSFKSPRFLPVLSKVAWKYRGQIIPQNKY
                     MQLDLHVKNIEQKEGQVVLTADANLWREDLRIYEIFDIVVGISETEK"
     gene            complement(22075..24549)
                     /locus_tag="EYO68_05345"
     CDS             complement(22075..24549)
                     /locus_tag="EYO68_05345"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08659.8,HMM:PF14765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC07265.1"
                     /translation="MVPLAPPDVLHAELPENPLCLITDDGSSLAPVLAKALSEQGWSP
                     VILRFSGISELVKKKRKPFAKEIPLIELSSSAESELETSLKSLTEKRGNIGGFIHLHP
                     ASSAPAESKLEDGANAFLKQAFLTAKNVCTFLNEAGKSDSSRSHFVTVTRLDGELGMG
                     SGQFGAVGSGLSGLIKTAGVEWPDVFCRFVDLQPELTADTAASCILQELLDPDLRIKE
                     VGYSGSGKSGTRRMTVQPKIIRDLTTKNSSKSLTKKSVFLVSGGARGVTAECVVKLAQ
                     TQPCSFILLGRSTIEDEPEWAKGVDEDKTKLKQAAMKSLVDSGEKPTPAKVNQLVGKV
                     EAGRDIRKNLERISNAGGNAEYVSADVTDAKKLKTAIAPVVKKIGPVTGIIHGAGVLA
                     DKLIEKKTSDDFDAVCSTKINGIDALLKSINPKKLTHLLLFSSAAGFYGNAGQSDYAV
                     ANEALNRIALLFKKNNPKCHVTSFNWGPWEGGMVTPELKRLFEERNVEVISVEDGTRV
                     FVEEVTSAGQENPIVLIGNSMVVPGEPGNGLRKWKISRRVNLEESPVFQDHKIGKNPV
                     LPAVHAMAWMADVCAQELPGFRLSNCENFKVLNGVKFDESLAAEYILELQEIERLNGN
                     FTDVEVKVSSLSGSESGSKKLPRFHYSTKVRMVQQVPEAPLHDRIDMSNSHNLPGSTF
                     YQDGTLFHGPKFQGIEQVLNIGEQGLTLECRLPDVSVAEQGQFATSDFNPFAADLAFQ
                     AMLIWAWRFHQSGSLPLKTDLLEHFRNVEPATRFFLSMSVNKSSASTLNANLFLHDEQ
                     GLLYTRMLGSEVTLSKSLNALFGKNN"
     gene            complement(24606..>25798)
                     /locus_tag="EYO68_05350"
     CDS             complement(24606..>25798)
                     /locus_tag="EYO68_05350"
                     /inference="COORDINATES: protein motif:HMM:PF00550.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HIC07266.1"
                     /translation="KIPALPEVPGDDLAEMRTLGEIVAHLQSKLGTDDSALQTAGTLT
                     SEITAAASAASSSGVSAEDITAAMMAIVGEKTGYPLEMLEPGMDMESDLGIDSIKRVE
                     ILGAVKDKIPELPEVPGDELAEMRTLGQIIDHLKSKIGRDVSEASREIPSTVAIPDSE
                     LNTQNPTPDTGQDPGTVFMSIISEKTGYPAEMLELGMDMEADLGIDSIKRVEIMWSLQ
                     EQFEDLPQIGANEVAELRTVGQIIEHIKSLLPAFGGKDVVQKSNLDLEPAPLSVSAGS
                     PGGNGNGSGAVSSDPVPGEIAPALLAIIGEKTGYPAEMLELGMDMEADLGIDSIKRVE
                     IMWSLQEQLPHLPQVSGSEMSELRTLKEIVDHLSSLAPSETAGTTIIPAPVSTDSDSV
                     KKNS"
BASE COUNT         7340 a         5326 c         5563 g         7469 t
ORIGIN      
        1 aaaaaaactt gactattatt gtcaaacaag ggaaacacag caatattttt atgatttcat
       61 caggataatg aaatgagtga ttttgatact gagctaaatt gtgagggact taattgccct
      121 cttccggttt taaaaaccaa gaaaaaaatt gatggaatgt cgagtggcga aatattgaaa
      181 atgacttcga cagaccccgg ttccgtgaat gacgttactt tctggtcgaa gattaataaa
      241 aattatttgt tgtctcacgc tgaagatggt ggtgtatata ctttttatat tcagaaaaag
      301 tagatttatt taaagttatt aagaaagata atttttgtgt gacttgttac acaatcaagt
      361 tgggaacaga atctcggctt tatcgaggag caggaaagac aaacaagtgt ggtattttga
      421 ctttttacga aaccatcata aattatattt taatttttaa tatattagtt aacttattat
      481 ttcactcata agccccattc cacatgttct cagcacagta aagcaccgta tcgacctcag
      541 cgtaacctca cttgacaaat gtcaccatga gtactcaacc ctacatcggt ttccgtcagc
      601 tctttgatca ggacacatcg accttcactt acctgctctg ggatttggac accagggaag
      661 gggtgattat tgatccggtg agggaacagt ttgaacgcga tctttgtctt gttggcgagc
      721 ttggtgtgcg tatggtctac gcgctggaca cgcatgtcca tgccgatcat gtaacgtcgc
      781 tgggaatgct gcgcgaagag atgaggctgc aaaccgcggt tggagagcct tccggagttc
      841 cctgcgcgga catcatgctg aatgacggtt acacattgcg ctttggcagg cataccctta
      901 ctgcgcttgc cactcccgga cacacagacg cctgcacaag cttcaaggtt gagaacatgg
      961 tgttcacggg cgatgccctc ctcatccgag gctgcgggcg cacggatttc cagcagggcg
     1021 atcctgaaat gctgtatcgg agtatcacgc aaaagctcta cgctcttccg gatgaaactc
     1081 tggtttatcc cgggcacgac tataatggta agtctgtttc caccattggt gaggagaagc
     1141 agtacaatcc gcgcattcca gccacacaaa ccgaatcgga ttttgctgaa ttgatgaact
     1201 cactgaacct gccgaggccc aaacacattg atgaggcggt tccggcaaac atgggctgcg
     1261 gtatttcagt ggatcacgga cacctgactg aggaagtatt tggagtacgt gatttacaga
     1321 aaatcctcac tgctctgtcc gaggacgagg tggtgatcga ctgccgcact ccggatgaat
     1381 acgaggcagg gcatattccg ggcgcgatca acctgccgat gggaaaggaa ctggatcagc
     1441 tgggtgaatt gcgggattac cgaaaaatct acctctactg ttattcagga cggaggtccc
     1501 agacggtgtt tgccactttg accagcaaag ggctggacaa cttggtctgc ctgggaagct
     1561 ccgggatggc ggagtggaaa aaattcggat atcctgtgga gagatgaacg aacacaggca
     1621 accaaaacag ggagaataaa atgacagaat ttgaagtggc tatgccgctt gcgggaggta
     1681 tactgattgg gattgcggca gccatgatga tgctgttcag cgggaagatc gcgggaatct
     1741 cagggatatt cgggggaatg ctctttcagc agggagagga gcggacatgg aggtcagcct
     1801 ttgttgccgg tctgattgcc gggggagtcc tgctctacag tataaacacg gaatattttg
     1861 aaaactcatc cggacgaggt cttgtggccg tgacaattgc cggactgctg gttggtatcg
     1921 gcacacgggt tggcggaggc tgcaccagcg ggcacggagt gtgcggcatc ggcaggcttt
     1981 cggcacgttc acttgcggct acggttacgt ttatggtggc aggaatggcg atggtggccc
     2041 ttgttcaacc cctttatcac tgagaactaa gatggctaaa tcgaatattg cagcctttct
     2101 ttgcggactt cttttctcca ttggtcttgg tatcagtgga atgacacagc ctcagaaggt
     2161 cattggcttc ctcgatgtct tcggggaatg ggatcttagt ctggcttttg tgatgggcgg
     2221 cgctgtgatc agctatttat tgctccaact atggattcag ctacgctttt ccaaaccgat
     2281 gctgggggaa acgtttcaaa ttccctcccg taaggatctt gaccgttcac ttatctttgg
     2341 agcgctgctg ttcggcgtgg gctgggggct aggtggatac tgtccgggac cggcgatcac
     2401 ctctctaggc agcggaagcg tgaatgccgt gctgttcgtg gtggcaatgg gtatgggcat
     2461 gctgctggca gaccgtgtga tttctcccat tcttactcat caggcgctca aaagctgagt
     2521 ctgtttatcc gatatgacaa atgaagcgct ttttcagcaa gccctaatta agtccgtgtt
     2581 tttgttgact aagcgcttag agaacaccat cttactcaca ttcaaatgac ctcaactaca
     2641 aggaaaacga tggcattcac ggtaaaacta ttgatcaacg gagaatcagt tgatggtgac
     2701 ggtgattcat tggctgttca gaacccttcc accggaagca caatttgtga ggttgcggaa
     2761 gcaacagcgg aacaggttga agcgactgtg cgggccgccc gggatgcttg atatatttta
     2821 ttgtttgaat atacaaaaaa aatccacgac ttcctccttt gacaatttgc taaagaatcc
     2881 agaaaaaaca ataacttcga tcatcaagcg agttgctact gggcctgaac tcagcaaaag
     2941 tatttcttat aaagagtcaa gggccagtat gcgtcttgtt ttggagggtc tggcagatcc
     3001 agtccaggcg gcaatgtttt tgatcggttt gcgaatgatg cgtgaaactg atgatgaata
     3061 caaacggatc ttgcaggaca tccgtaacaa aacaaaaacc gttgtcgctg atgtagatga
     3121 attggtggac atcgccgatc cgtacaacgg ttatggtcgc agtcttccgt cttctccatt
     3181 tttgccggta ttgcttgcgg aatccggagt tccagcggta tcgcacggag tagaaacggt
     3241 tggaccgaaa tttggagtga cgcaccacca gattttacag gcagccgggg tttcggtgga
     3301 gttgactgga gaagaagccg gaaagcagat tagtaatccg gaaattggtt gggcctatgt
     3361 tgatcaaagt caattttgtc caagcctgtt tggtatggca gagtttcgta agaaaatagt
     3421 gaaacgggca gccatctcaa ctgccgaggt tttgaccggt cctgtacggg gccgtaataa
     3481 aacacactta ctgaccggat atgtgcacaa tgagtatccg ccgatttaca ccatgctggc
     3541 caggcattcc ggattcaatt ccgcgctttt gatgcgtgga acggaaggcg gggtgactcc
     3601 gtcattacgc aaacgtggtg aatttgtccg ctattgggag cagagtgaag atgttgttgt
     3661 tgaagctgat ccatcgaaaa ttgggattga gcaggaaaat cgtctggtgc caattgctca
     3721 tgcgctgatg ccaataatag atagagaatt tggacctgat gaaaacaatg ctgaaattgc
     3781 aaaacttgcc gcaaaggaag gtctggcggc tatagaagga aaaaaaagtg ccacctgtga
     3841 tgccttgctt tacagtgcat cgctgacgct ttggcatttg gggcgttatg actctgttcc
     3901 ggatgcagct tcgatggtgc gtgaaatttt attcagcggc aaagccgccg cacgtttccg
     3961 aagagcggca tgagtgaatt atttgtaaac accttcttct taaatttatt gaataaaata
     4021 atttctttat tacgtttgaa tcgaatattt ttctgcaatt tagaccggat catgttaaaa
     4081 taagctcgct tatgtaactc aaactcaaac ttataattcg aatggtgatt tctggatggt
     4141 agttccctta gtttgctcat ggcagtctat gaaaaaattg acggtcggag atgttttgca
     4201 aatgagcagt tctcatcctt gagctgagcc agatataaag gcctggtgca ggacttccgg
     4261 acacaaattg ctgcaaatcg tgattgagaa tggagagaga cactttttcg tgcgttgtgc
     4321 gaattaagag agattgaaag taaacgcaga ttaagaataa tacattcact agtttcgttt
     4381 gaaagttttt cattcatgga gaaaggatag agccttgaat acaatcgaaa ccataaaaga
     4441 accggaaaat ttgaaagaag tatttgcttc cggttatgca aagatgtttg gtaagcaatg
     4501 gccgatgtgg gttggcggac ttttgtttgg agcactcaac gtttttatgt ttgcctacga
     4561 aaaagcctgg agcgtttctg atggcgttag aaactgggga aactggtttt tcaataccat
     4621 tgaggtttca gagatgatta ttattgaacc ccaccttttt accacttcca ttttaaattt
     4681 cggtatcatt gtcggtgcta tggcttcggc acttttgtca aagcaattcc aggtcagggg
     4741 tgcccctgcc cgggagttaa tcaaagctct gattggcgga atattaatgg gaattggctc
     4801 agtcctgtct tttggctgca acattggagg attttttagt gcaatcagtg cactatccct
     4861 tgcaggagtg gcaatgatgg ccggactcat gataggtgcg tttattggtc tgaaacttct
     4921 agtttgggaa ataacgtatt taagtccagc ttcttctcac gcaagcagga aaatatcagg
     4981 tgaccggaaa aagaatcagc ctttagcggg cataattgtt ttgctgttag gagtcagcct
     5041 tgcctttgtt tatgatgatt tggattattc aatccgggga ggttttttag tctttgggtt
     5101 agtgataggc ttgctcatgc agcggactcg tttctgtttt gtgcgggctt tccgtgaacc
     5161 ttttttgtct ggagatggaa atgctgccaa ggcggttgca ctcgcggtga ttatcagtgt
     5221 agtcggattt tcagtactaa aatggtcaga catgaaagat tgggaggaaa ttgtgtatgc
     5281 aggattttgg ttcgggagtc tggtgggagg aattattttt gggatcggca tgtcccttgc
     5341 cggtggatgt gcttcaggtt cgttatggcg ggccggagaa gggcatatta agctttgggt
     5401 tgctttgatt gcatttgcat tgtcaggctc atattttaga gaatggctgg ttgaaagtgg
     5461 ctggatcatg aaattgggcg aacctctttt cataccggat ttaatcggct ggaaaatggg
     5521 gattttgtca atatgcattc taatgcttct ctggtatctg attgtttcat ggaatgaagc
     5581 aaccagaaaa ctagtggttg tctgaacatc agtttttata acctgctcca aatctcacaa
     5641 agagattggt gtttttcttg ttaatttatc attggaaggg aggactaata taaaaaaaat
     5701 tactggaatt ggcggaattt cccggcaatt tgaataattc aaaaacaata aggagaagat
     5761 tatgaataag caattaaaaa taatgttaac tgtctccatg ttagcaggtt ttcttggagg
     5821 ttgtgtaact ggaacaaaaa tgggagcgcc gacaaaaggt atgagcactg aggagtatca
     5881 gtcactcatt ttcctggatc cgcaaaatac gctccggaca aactgggatg ccgtaaggga
     5941 tacgcccgga tattttctga ttgataacca gggagaaccg acagtttcgg tggttgatta
     6001 tcataaagga gaaacaatca agaaaattca aatgggtgaa acaggaaacc accacatctt
     6061 tgttatacct ggagcgcgat atgcgttttc gtctcagcgt tatgagaagg atttaatctg
     6121 ggtaatcgac ttaactactc atgaggtagt tgatacattc catctgagca tgggcggaaa
     6181 gaaagtaatt gcgcctctgc acattggttt tgcttacact cagcctctgg cagttgtggg
     6241 taacatcttg gacaaaaaga acggttacct gtcgtttatc aacacggcaa cccgtaaacc
     6301 atcacacatt gttgagctag gctgtcacgg ggcacgtgat gccatgttca ctttagatga
     6361 ttccaaagtg tttaccactt gtcagcagga gccaaaaggg gtttcgattg ttgatgtgaa
     6421 aagcagaaaa gaaatcaaag tcattcctat taaaggagga agagctggtg gaatgacacc
     6481 ggacggtaaa tatttcatgg ttggtgcttc aaaggctatt gtcttcattg atacagcaac
     6541 caatgaaatt gccaaaacta tcaaggttcc gggtggtgga ggaaacttca cttgtcttcc
     6601 ggatggttca aaatgttacg gtggtttgag aaaagacaat tcagttgctg tgatcgatat
     6661 ggcaaaactt gaacttatca aggtgattaa aaccggtcca aacgcaaacc ggctttatct
     6721 taactctgca aatccccgtt acggattgtt tacaaatgag tccggaaaat cagagcaagt
     6781 ctctgtgatt gacacccagg atgatgtcaa gatcaaagac atcattactg gtttagggcc
     6841 tcataatgta gcctttgatc caacaggtaa atttgctctt gttaccacaa aaaaggaaaa
     6901 tgtagcaacg ctgatagaca cctcttcagc aaatcctgct gactgggatg tgattaccac
     6961 agacattgct gcaggcatcc aaaataatgg tgtgcgctgg gttcctagcc caggtgcaat
     7021 taaagcagca atggcaaact aagtaggatt ggggaattgg ctgtagtttt ttcagttttt
     7081 aaaaactacg ccaaccccca atttcattat tcacaaaacc ttctcataaa agagattgtc
     7141 tcatggtaat taaagaagat ttttccagaa gaaagttcat tcgttattct gtgattggcg
     7201 gggctgttgt tcctctcctt tccggagttc cttatgaaaa tgaagtgtat ggcggagttt
     7261 tcaatgctcc aaaagattta gagaatctga ctgagataga acgcatccac ctgccaaagg
     7321 tcatcatgcc accggtagtt gaggatggca cacaagcatc tattgtagtc agtctggatc
     7381 atcccatgga agaagatcat tatatcaaaa gcattcagat attaaatttt gccgatcctg
     7441 tggtcatcaa gggaaaattc tacatgactc cgataaacgg agaagcgttt atcagcacac
     7501 aaatacgtct tgctgggggt gagggaaccg tttgggttgt tgcagaatgc aacaaacatg
     7561 gaaaatgggc ggcaaacaga acagtcaatg tcgctgctgg aggatgttga agtggtcttg
     7621 aaattcagga aaatgacaaa aacaatttgt ttttgttttt tgacactgat tttttttggg
     7681 ccagtattca tatcagaaac tgcttctgtt tttgcatccg aaacagaaca aataaggatt
     7741 atttctacca acgatattca ttcctattta cgcccgattt attaccgtta cctggatgag
     7801 ttcaaaccgt ggggggcaca gtcaagagaa ggtgattatg taaacaaggc tgaaatagaa
     7861 ggtaaaattg gcgggatggc acatgttgcc acgatgatca aaaaactaag agcagagaag
     7921 cccggaaaga atctgctgtt agatgcaggt gatacgtggc atggagcggg attgtcactt
     7981 tttgacaaag gtgtttcgat ggtgaaaatc atgaacgcca ttggctacga tgtcatgacg
     8041 ccgggaaatt gggagttttt atatccgaaa gatcattttt tggatttggt tgatcaagct
     8101 aattttcctg taattgctta caatttgact gacagagaat gggaggaacc tgttctcgac
     8161 cagtatgttg tcaagaaagt cggcaatttg aaagttgccc tgatcggctt gacttatccc
     8221 tggactgcct taacttcagc aattaccggt gcagcacagt ggttcaagtt cggaatcaaa
     8281 gaagaagaag cacgcgaact gattgaagat atccgcaagc aggagaatcc agatcttgtg
     8341 gtcttcgttt cacatggcgg ttacggcctg gatcagaaat ttgctcagcg agtggatgga
     8401 attgatatct atctcagcgg tcacacacac gacgaggtat ttgatcctgt tgtctggaac
     8461 gacaccatca tcttccaggg cggtgcacat gggaaatatg ttgtcagtct tgatgttgat
     8521 gttaaagata agaaaataca aaattttgat tatcgtttac tcaaagtaag acagaatcaa
     8581 attgagcctg acccggaaat tgaaaaactg attgaagaag catacgagcc ttacaaaaag
     8641 cagttgagcg aagtggttgg gcagaccaaa gtcatgctgc accggcggga ctattttcag
     8701 agcagcatgg gaaatttgct gactgatgca atgagagatt ctcaaaaaac cgacatcgct
     8761 ttttttcctg cttggagata tggagcgaca atgatgccgg gaaaaataac tgctgaagat
     8821 gtttataaca ttgtaccaac agacggtcat gtttttacat attctatggg aggaaaagga
     8881 ctaaaaactc tgctcgaaaa tattttgggt tcagtggtca ataaagatcc ttacacaaga
     8941 gttggcggag atatgattcg tttttccgga atgaaacttg ttgcagattt agcaaaatca
     9001 cccggaaata gaattgtctc attgacagtt ggcgggaaac cattctcgaa agatgcagtt
     9061 tacagcgttg cttccgcaca cacaaggttt cagaacggcc ccctttttgg ggcgtcgaaa
     9121 gttaaggaca caggaatagt ttttgtggaa gaattgatca actatatccg caagaattca
     9181 ccaataactt ctgctttgga tgatagaata aatattttgg gatttcaata agttaatgaa
     9241 gatatctaaa taattttaat ggagaagaac aatggcaaaa acaatccggg atccacgcat
     9301 aagaatttcc aggcgtatca aaaaagataa agtgtttaag gtcaaagtta aattcgacca
     9361 tccctccttt accggcttgg gaattgttga tgatgaaaca aagccggtat ttagcagggc
     9421 aattcccgtt tccttcatta gaaacatgtt tgtctatttt ggtgatcaac tggtcagccg
     9481 ctttcgcatg tcatctgcaa tagcagatga tccattgttc acgtttaaat taaaagccat
     9541 tcaggaagca cctgtcaagg tggtttttgt ggacaatcaa gggaaaaagt gggaagcgtc
     9601 caaaaagata aaattcagta actagacttt ttagaaaaaa ttatagggcg gttttactaa
     9661 gtgcatcctc cattttgtta gaaaatgaac caaagtgttt atgaaaaaaa tatttaggtt
     9721 tattattttg tcattcttct ttttgatcat gggttcccat gctgtttttg ccggaacaat
     9781 tgaagaggag tttaagatcg aagggttaat ctcgcctgca agtcccaagg ctttgaaatc
     9841 cgcgctggaa aaagaattag aggttaaagt aattgattta aatctaaaaa atacaaaaac
     9901 cggatggccg gttcttactg ttcaatttga ttccggagca gtctcaagag aaaaaattga
     9961 aaatgtcatt ggagagattg aggatcctgc aggacataag tacaaggtac atcatggtcc
    10021 ccttctggcg aatgctccga ttcttgatga agaagaggag gctatcgcaa tcctgggtga
    10081 tactgccgaa gtgtttccaa acatgaagaa ccccattacg gataaagcag cttcagcaag
    10141 tcgtggggga aagttatttg taaataactg tgctaaatgc catggtttaa gtggaaatgg
    10201 ttatggaaca gttgctctca gcttcactac ctggcccagg caactgtggg catggaataa
    10261 tactggtcca gaaacagacg gatatctatt ttggtttatt actaatggac gttcggatat
    10321 gccgccgttt ggcttgattt tatctgaaaa taaccgctgg gatttgataa attacattaa
    10381 aacaatcaag aatccaggtg aataattagt gtcctggcag tacctgtata attcattcaa
    10441 actgctttaa acctcaatct ttgtatgcca ggttggcgat tgtgtaacac gacctgcttt
    10501 tggtgtaata gtttttcaga gtactcgtaa agaaaaaaat ccgacgaaag caacctagaa
    10561 aaatgacacg atggaagttg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 ngtaaagaaa aaaatccgac gaaagcaacc tagaaaaatg acacgatgga agttgctcag
    10741 gatattgtca aaaaggtttt tatggggaaa aatttcaaag atcttcttga tgatctgatc
    10801 aaagaaattg aagtacgaaa ctatctgatt aagcgcatca gcaatataga taatatccac
    10861 aaaaggtata gtcaaggtat tgctccggat attgatttcc aacattacaa aatagttgaa
    10921 ttttgtaacc tcgaaagttg cagcgggcta atttcttcaa attttcttgc cggtgttttt
    10981 atgccagtaa gattcgtcgt tttccagcgt tttgagcaag aagaaataaa tatcgcgttt
    11041 ctaaaaccga cttcctttgc ccgccttttt aactccaata aaatgatgaa ttttgcaaaa
    11101 tcattagagc aggatatgaa ttatgtgctg gaagaaattg ttttttaaga gcaaaatatt
    11161 tttattggtc tttgtgcctt tatttttctt atctgaatat atttttatac ttacggcaca
    11221 cgaaaaaaaa gtgtcaggga caaataattt tcatggtgga gattttactt taaagtctac
    11281 agaaggccct ttctcactca aagatttacg tggttcggtt gtcctcttgt ttttcggtta
    11341 cacctcctgc ccaagtgttt gcccaatttc cttagccacc atcagcaatg tcttttccca
    11401 aatgtcgcct gatgagttga aaagaacaaa ggcactattc attagtcttg atcctgaaaa
    11461 agacaatctt gagatactca aacaatacac agattatttt catccaaaca tagtaggact
    11521 tactgacgat attaaaacat tgaccaaagt tgcaaagcaa tatggtgtaa aatacaaaaa
    11581 aacacttgtt ccggattctg cgttaggcta cgtgatttct cactcagatg atatttatgt
    11641 ggtcggaccg aacgggaaac cgcaaaaaac attcccgcat gatacgaata cagttcctct
    11701 tcttgcgcaa attaaacgac ttttggtagt aaattgatac cagttttgtt atcaataata
    11761 aaacgtgtaa atcacctgga aagtgatcat gaattcattt atcaaattta gtttatgtct
    11821 tttggtgttt ctccaattga atgcaacatc ttttgctttt gagcatgagg attctgatgt
    11881 ccattttcta gaatatgatc ccaatgtcat ccaaaagaac aggtttaaga aaaaaccata
    11941 ttttttacta tttgccgctc aatggtgcca ttggtgtcat gtcttcaacg agaaaacact
    12001 cactgatgaa aaagtaattt cttacctcaa cgaaaatttt gtcaatgtgt ttatcgatgc
    12061 agatatcaac acaagtgctt atcaaaaata taaggcaaaa ggagttccgt ttacagtatt
    12121 tttgaatcct gacacatcag aatattataa gtattccgga accctttacg cagagccttt
    12181 tcttgaagtc attcaggatg tgattcaaaa tgtgaaacaa ggaaaaactg ttgatggaga
    12241 gaaaatattt gcatttgaat ataatccgcc tacaaaattc aataaatcaa ctttagataa
    12301 tatgcgcaat acttacatta aaggcgtttt ggataatttt gatactgaag aatatggagt
    12361 tggaaacgga ataaaaacaa tccttccgga aactttcctc tatctcataa aatctaccaa
    12421 aggagaaaat cgacaggact ctgttttatg gatttctgaa acactaaaaa aagcaattga
    12481 gaatatttat gatccaattg aaggcggatt ttttcgtttt gccgaaaccg gtgattggca
    12541 agtacctcat tatgaaaaaa tggctggtct taatgctgga acagttttac tcttgtacaa
    12601 agtgaatgag gaaaatccaa atccaaaatt cattgaagtg gctctgcaaa gtattcagta
    12661 tttgtctaat acattatacg atgaggagac tggttctttt ctcagttttc aagcagcaga
    12721 cacatcgtat tattttttga atgagaatcg ccgaaaaaat gtaaaaccgc ctttggtaat
    12781 cagtaaggtt tttacagatc atttagctgt aacactcact tatttcttgg atgtacttga
    12841 ttattcaaaa gataataatc tcgaaaacaa agtgatccat tcacttgatt ttctttccgg
    12901 aatgattctc aataacaagc gaatttatca cttttatact atcaaagaaa aacagtggcg
    12961 tggtaaaagc ggtcttcagg atcatgtttt gctggcaaaa ctgtttcagc gggcggcgga
    13021 caaattccaa aatgaaagct acaataaggc ttattcaaaa atgcttcgat ttttgaagtt
    13081 gaattattat gatgagaaaa aacaaatttt tgttgatccc gaattagata caaatgatta
    13141 tgagtatttg atggctatga acggtgatat tgtatttgcc ttgatgggac aaagtaagaa
    13201 atctgctatt ccacagttga gtcccgtgaa accgataata acatattttt ctggattgga
    13261 tgagttcctt gaagataaat tttgggatag taaaagctgg gattttcttg aacggtctgc
    13321 atctttttta tcttcagcag acagtttttt ggcaactcaa agcaatcctt aaaatatata
    13381 catattttaa taaaggtact cgacagtcac aggttatttc gtgttatccc gaatgtgtgt
    13441 gataaattat atatgatatc agcatattat cggatttatc cttttggtcc agatgacata
    13501 ctgcagtaaa taattgcaat ttcttacgag atttcacaac tcatgagtgt aggtaaatat
    13561 atgaatattg gaaatcattt tgaatctact gaaagggggt ttgttattcg aaactttgca
    13621 ataattttga ttcttttagc tggaataagc ttgtttacgt ctggctgcgt cacactggga
    13681 aaagactttc ctgaggcaaa tgtatctgtg atcaagattg gtcagactac caaaaatgag
    13741 atccggaagt tgtttggatc tccgtggctt tcaggaattc aggatggaga actggcctgg
    13801 acttacggta attatgatta ttctcttttt ggtaaaaggg aggcgaagga tcttgtcatt
    13861 cagtttgatg aaaaaggtgt agtgactacc tacactttca gtactacgga tcacgatgaa
    13921 taagtgcgtt tgtaagaact gtagtaaaag tcttttttaa atgatccttg tcatttactg
    13981 gaaaaacaaa tagtttatga attttaggaa gaaaataata tgaatggtga cacattagca
    14041 aaaatcttac tggttttgat aactctgtgc tttgcaactt ttgcttcagc tgcagagaaa
    14101 gtaccattcg gttcagaacc gcttgctccg gaagaagctt ttgtgatgga tcacatcatt
    14161 gtaggacctt ccgaagcagt tattcgctgg caaattccaa agttctatta tctatacaaa
    14221 gaaaagttca tcttcaccag taaagatttt atcattgaga atgttcagtt tcctccacca
    14281 gaagttattg atgacccctt ttttggttca agtgaaattt atcataatgt cgtggaagct
    14341 acgttacatc ttactccaac aatcaaagga gaatctaagg gaattctgga aattggcttc
    14401 cagggttgtt gggagggcgg agtttgctat cctcctgtaa aaacttcatt aaaactgtcc
    14461 ggactttaaa ctgtttcaac tgaaattttt caatatagta caaggccttt ttttctgtaa
    14521 ccaaaatatt acgggcttgt tgaaaaataa tatttttcaa tgatatcaat aagaaattat
    14581 tcgattgatg aaaaaaactc caaggagttt ccaaaaaaac tgttttattt ttctgtttct
    14641 gaaattccaa ctacaatgtc aaatatttca tagattctca aatcttcccg ccacaagttt
    14701 gcatctgcag tcaagaccac ctgcccctct ttttgctcta tgtttttaac atgcaagtcc
    14761 agctgcatgt atttattttg tggaataatt tgtccacgat atttccaggc aaccttgctc
    14821 aaaacaggca aaaaccttgg acttttgaat gagtttccaa ttccctgctg caaggcaaaa
    14881 gcctgcaaag cttgaattat tgcttccact ccaagtgaac cgggcataac tggatccccg
    14941 tgaaaatgac aggggaaaaa ccagtcttcc ggattgactt ctttgcgtgc ataaacatag
    15001 cccttgctga attttccgcc ttccggcacg atccggatta tatcagaaaa actaagttgt
    15061 ccggaacata aacgaaaata aggatgttcc ggtttttctc ctaaagcctt ccggaaatct
    15121 acggaattca aatcaagtaa aattgaagac acagaaggag tttcatcaat ccacggaacg
    15181 gcttttttac ctccgtctaa accaacttga gcagccaatg actcgccggt aaaatagcca
    15241 aacattgtat caccctcaaa aaatatttgt ccatcacagc tcaaagaaaa acgatgcgtt
    15301 tgaatgatag tgtttccgga ggcaaccgtg gttagtaact tgacttcatt caaaaccgtt
    15361 tttccgcgta aatccggatt cctgagtaaa gttccgtttc cgtcaaggtt gcggtaatac
    15421 aagtcaagtt ccggataagt caaaattgct ccggaatgag ttgaaataaa accgcacggt
    15481 tgtaaagcaa tttccatcaa aacggaatac ggcatgtttt caggataaga attattacga
    15541 aaaaaccagg catcttcagg aacgtcatat tcactgacca aggtcatcgt cttgtcaaac
    15601 tccatccttt tgccatgcag atcatagact cgtgaaataa gctgcaaatc gccgttcgga
    15661 tttcgctgca tggaacggcc tttgtagatg gcaaattcgg aaccgaaaca tttttccaca
    15721 tctcccaaag caaattccca aatcatctgt tcgtctgcag caagctgcct gtcactagtt
    15781 tccggcactt gaatattaga atccggtgtt ttgatttctc caccggaata tgctttgcgt
    15841 tggtttttag aaacacgaag caaattgccg ccgccggtgc ttccggtttt ttccgccagt
    15901 tgaactccca ggtctcgaaa atcaaccacg attctgtcgc caaccaaaat atcgatgtct
    15961 gcaattgcat acggatgtgg ctccaaaccg atctctttga tttcaacccg ataggtcatt
    16021 ttaggatcgc ccggaatcac ctgcccccgg caacgtacaa tttgcggcaa cccatgaatc
    16081 ggttgaaatg ttgcgtcctc cactaaggtt tgcaacccca gatgaagaag aaaaaactga
    16141 agcagctgta cacagccttc tgccatcagg gatccagcca taaccggatc atccttaaaa
    16201 tgacatggga aataccagtc atccggagct aaatcttttt ctgcaactat gtatcccaga
    16261 ccatacggac caccatgcgg ctctactgac ataattctgt ctgacatcaa aaactgatcc
    16321 ggtgcatttt ttaatgatgg atttttcccc tgctgattat attccggacc aaaacatccg
    16381 gcaggatttc cggctgaaat ttcgagtaat tcttgtcgag taaatgttgt ttttgtacag
    16441 tgaagaagcg gcgggaattt gattttatcc gctttttctt tgagctctaa ttcctgtgct
    16501 gtatgaatca cacctttgcc ctgagataaa tcttcatctg agaaaaatcc cgcacagccg
    16561 ttatccatct tgagtaccat tttgttgccg acaaaacact cataattaaa aaagaacagc
    16621 aaattctgat catgttttgc aaacgaattg atagatattt cgtagcgcaa agtttgtcct
    16681 tcaagcggca tgtcgtcaag gtatgtcaat gtacaatcca gcagtcggta aacttgctca
    16741 cctttgcagt caaaatcaat acccaaataa ctgatcagca tcaaatcaca ttgtccggac
    16801 tctactgcaa ctgcccacgg aatttgaccg tcagttgtgt accacgcgtt atctggaatg
    16861 tcgtattctg tggtgatcgt tgaaggcttg aactctccaa gttttgcgtc aagctgtgtc
    16921 acacggctga ccagtaaata tggttccatc ggtaatcgca cacagcgtcg gtaggaatca
    16981 atttcagcat attcggcacc aaaaacattg gcaatgctgc cgcctgcaag ttccagtaaa
    17041 tcatgatagt caaaaatgca gggttttgtt tgtggtacag gttgctgtgt ctccggtgtc
    17101 gttttttgag aaactgtatg ttgaaaagta ttggaaactg aaattccttt tgtgttcgta
    17161 tttgaactga tcaattctgc cagctgacgc attccatgct gtcgaacatt caaaaatgct
    17221 gagtgtgccg aatttcggtg agaaccgtat tcacccggaa atggtgaaaa agaggaaaca
    17281 gaaacattcc ccggtgcgta gtccggggct accaaatttg aatatgcacg gccagacgcg
    17341 ctcccacttt gaaactctga aactttgaaa ctttgaaact cagtgccttg atgccttagt
    17401 gccttagtgc ctttaaaaag tttatcagca cctttttcaa aaacttgctg aactggagtg
    17461 cctccaagtt tgatagtctg cataagttgt ttttccgctt ggacaggctg ggacgggaaa
    17521 agcgcttcca gcttaaccgg aactcggtga cttatcagac tggacaaagc ctgaagaatc
    17581 atttctctgt tgtcagtacc cttacggtta acactgatcg aaaggtgagg ccgtttatcc
    17641 aaagttttac tgatccactt tgagcaggtt gttcgaggtc ctaaatccac aaatatacgg
    17701 gctccatctg cataaacttt ttcgatgaga cgactataat ccacggtttt tccgtaaatg
    17761 gtagcaacat tgtgcgccaa tgttttgctg tctaattttg aaacttcata atcgattgca
    17821 gaataaaaat caatatccgg acatttaaca actttatctg tgtgaacacg tttaatttct
    17881 tcatattctg ttttgaccgg ctcacagtgc accacatctg tgaccggaat tggatgtaca
    17941 ggccgtccca gttgctgaat cacacgctca caagctgtcg gttcgcctgc aatcacaact
    18001 tcttccggcg tgttgatcaa aatcagataa acatgtgctt cgttttcaac ttcctgctgt
    18061 acaatttgtg gagaaagctg gagagaataa catccccaaa gtggtttttg tctaaattca
    18121 tcaggtccca aatcccaggc ttcacgtact gcattcatgg gtccggccaa cctttcttca
    18181 aaaagcttgg atctattcag aatgtcgctc atatttgaca tgctttccca cactccaagt
    18241 ccatacagca tagaaatttc gcccatgctg taaccgaacg ctatgtccgg ttcaagacca
    18301 aagcctttgc gcataacatg tgtattcaac actgcagagc tgaccccgct ttcaaacatt
    18361 gcaataggag tgtggataaa ttctttctgc aactccgtta cttccgcatc agtcaagcgt
    18421 tccaaggttc gcggatacag ccgccgttcc tggagaagtt cccccaaata attgcttccc
    18481 cgccgtttat ctgcggggcc ggtctgattc aggtactgtt catccaactc gtgtagaccc
    18541 ggaaacattt gaaacaaaga acgtccacaa tcgacatacg cactaaaacc tcccggataa
    18601 gtgaaagcaa ccttcccctc ccgcgataaa ggtgatgcag taaaataact gccacgtgga
    18661 gaattccaat caccgctgcc ggtaaaagaa gtttcaattc cggattttgc tgcatcaatt
    18721 tctttttgca gttcatcacg actagagccg atcagtactg ccgcaaattt ttcttcatta
    18781 ttatttgaac gcacataata ttggtaagca agagatgccg gttctttgcc ggaaagcaaa
    18841 tctttttcta attcaagcaa ccctttgctg attgcggatt tttcctgccc tgtcacaaga
    18901 aacaaattca agtcgccaga ttcggcaact ttaattgttt gacgtaattc gggtgatgct
    18961 tccgaaagta tcagatgtga acaaatattg tctatgccca aactgctgac ggctgcatgt
    19021 ctttgtttaa ttcccgattg gatcagccat ggacgagatt ccaccggaac ataaaactgt
    19081 tttgcggact gcagttctgt tttgggtgac ttccactcag gaactccggg aatgaaacga
    19141 tggtgcaggc aaagcgcagc cttaatcaaa cttgccattc cggatgctgc aaaagtatgc
    19201 cccacatttg acttgatgct tcccaaagca caacctgcgt caactttagc ataagcttga
    19261 gccaacccgc tcatttctgc ggaatcttca acagtgtttc cgctgccgaa aacttccagc
    19321 attccaatct caccagccga gacatttgcc tctgttaaag cctgatttgc ggcgttttca
    19381 accgattctg gagttaaacc agttgagaaa gctattgaat caactgttgc aaaaatttgt
    19441 tcatcatcct tttttgctga ttctacactt tttaaaataa ccgtcccggc cccttctccg
    19501 atcaaccagc cgttggaatt cttgtcaaaa cttagcgtag cctcgccgga attcaggggt
    19561 aaatttcttt gacgcaacaa aacttgttcc ggactacccg caagatcaac agctgttacg
    19621 actactgcat caacattatc ttcaagcaac aagatttgcg caatttcgag tgctctcaga
    19681 actgagtttt cttcagctga aattgtcatt gcaggaccag aaaaatccca gagtgatgat
    19741 atcctggttg ccattatgtt gccgataaaa ctcgtaaagc ggttaattgg cacttcctcc
    19801 agcaaactgt tcctggacaa attgatcaaa tcttttcgtt cctcgtcact caaaacaatt
    19861 cctgctgctt gcaagctgtc ttgaatttgt gtagaaagat taacccgtcc cctgaaccgg
    19921 tgaatttcaa gttcagtttc catggctacc agtaccgcaa cattttgacc ttctcctaat
    19981 tttgtttttt taattgcccg gtctgttact tccagcgtca ataattgttg aggtatcaaa
    20041 cgttcttttg ggttgggctg caatttgaaa cgcagaaaat ccatttcaaa tgattcaagc
    20101 catgcaccct gaggatgatt ctttaattcc ggatgctgct caaaaccttt ccagcgctcc
    20161 ggcggaagag aacggaagtg ctgcttgttg tcatatatcg tttgataaaa ttcatgcaat
    20221 cctttgcatc cgccaaaaat tgcttccata ccgacaattg ccatgatctg tgaattttga
    20281 gtttttggaa aatcaattcg cttagaagcc ttaactcctg actcaaaaat aaggtgggca
    20341 tttgttcctc caaaaccaaa agcactcact gcagccctgc gatcctttcg attgtgtggc
    20401 cacactgcgg ttttttggac gatttgtttt tcagaaacac ctccattttt ggaagtcatt
    20461 gcagctttaa cacccctggt tgcaggtatt tctccatgtt ggagtgctaa aataactttt
    20521 atcattccgc ccattcctgc ggcagtcagc atatgaccca agtttgattt tgcagaacca
    20581 attagcggtt taattttctt tacttcagga aggtccggag acgcaaaaaa ttgttccatt
    20641 gagtccaatt ctactttgtc gcccagagga gttccagtag catgacattc gatatagtca
    20701 attttttctg gaggaacttt cgaggattcg taagcacgtt gataggccag aacctggccc
    20761 ttggaattcg gacttagcac aaattggccc cgtccgtcat tagacaaacc aattccggat
    20821 attaccgcat gaattttgtc agcatcacgc tctgcatcac acaaacgttt gagaacaaac
    20881 attcctgctc cctggctggc aagcaaccct ccagagttat tatcaagtgg attgaaagtc
    20941 tgaggagatt ccggaaaggc ctgaaagatc gaaaaaccca tattcacaaa aaacggatcc
    21001 gcggcactta ccgctccggc cagcattaaa tcggtttttc cagagtgcag ataatgacag
    21061 gcaagcgcga ctgcatacaa tgatgacgca caagcagcat ccagcactaa acttcgggca
    21121 gacaaaccca atgcttgtga aacaactgct gcaggaagtc cggaaatatg tgaattcagc
    21181 tgagcattat ctgtttctgc agcaaatgaa cctaagttga aatcaggacg attcagcact
    21241 ttctgcagat gaggctcaat cactttttgg tacaaaggca gtagtatatg attggatgct
    21301 ttggttggaa aagacaggtt acccagtact atgccgcatt ctttaagtat cttggtgttt
    21361 tctatataac cactgtcacg caacgcttca cgagccacgt gaagaggcca ttgaaatgtt
    21421 gctcccagtt tttcgatggt ttctgcagac agtagataac catctggatc cattttaaaa
    21481 tcattaatat aaccaccgcg tgcacaataa tatttatccg gagtcccttt tttctctgca
    21541 aaaaatcggg acggatccgc atccatgtct gcggcagcgg cagaggtaca tgaatctttt
    21601 tcctgcagta aatttttcca gtaatcttcg ggggtttttg cctcaggaaa gaggcatgac
    21661 aggccgataa cggcaatagg ttgcatttat ttattgattt ccaatttgct ttctgtgttg
    21721 ttcttttcag ctagccaata aattattttt gtcaatgatg aagaacctct aaaaagaaaa
    21781 aataacacac ttgtatgtca ttcccgcgaa agctggaatc cattgcttca gacacttaca
    21841 agtttctgga ttcccgcttt ggcgagaata acaattattt tcgacacaaa ccaaaccccg
    21901 aaacggagtt cggggctacc aactttgtag gggcacggtg agctttgtac gagaacagcc
    21961 ctctaagtac gggcacggtg cgacgtgacc atactttgaa tccttgaaac ttcttttgcc
    22021 attttactca gtgccttagt gtcttctttt actttgaaac tctgaaactc tattttagtt
    22081 attttttcca aataatgcat ttaaggactt acttagagta acttctgaac ccaacattcg
    22141 tgtatataat aagccctgct catcatgtaa aaacaggttt gcgttgagag tagaagcaga
    22201 acttttattg acactcatac ttagaaaaaa tcttgttgca ggttccacgt tgcggaaatg
    22261 ttccagcaga tctgttttaa ggggaaggct gccggattga tgaaaacgcc aggcccaaat
    22321 caacatggcc tgaaaagcaa ggtctgctgc aaatggattg aaatcactgg ttgcaaattg
    22381 tccttgttct gcaacagaaa catccggcag tctgcattcg agagtcaaac cctgctcacc
    22441 aatattcagc acctgttcta ttccctgaaa ttttggtcca tgaaacaaag tgccatcctg
    22501 ataaaaagtt gaaccaggca gattgtgtga gttactcata tcaatccggt catgcagcgg
    22561 tgcctccgga acctgctgaa ccatgcgaac tttagtactg taatgaaaac ggggaagttt
    22621 tttgctgccg ctttcagatc cagagagact ggaaaccttt acttcaacat ccgtaaaatt
    22681 tccgtttaaa cgttcaatct cctgcagttc cagtatgtat tcggcagcca gggattcatc
    22741 aaattttact ccattcagta ctttaaagtt ttcacagttg gacagcctga acccaggcaa
    22801 ttcctgtgca catacatctg ccatccaagc cattgcatgg acggcaggca aaacaggatt
    22861 tttaccaatt ttatgatcct gaaaaaccgg actttcttcc aaattaactc ttcgggatat
    22921 tttccatttt cgcaatccgt ttcccggttc acctggaacc accattgaat ttccaatcag
    22981 tacaatcgga ttctcctgac ctgcagatgt aacctcttct acaaacacac gggttccgtc
    23041 ttcaacagaa attacttcaa cgttacgctc ctcaaaaagc ctttttaatt ctggagtaac
    23101 cattccgcct tcccatggtc cccaattgaa ggatgtaaca tggcattttg gattattttt
    23161 cttgaataaa agtgcaatcc ggtttagagc ctcattggca acggcataat ccgactgtcc
    23221 ggcattaccg taaaaaccag cggcagatga aaacaacagc agatgagtta atttttttgg
    23281 atttatgctt ttcaaaagag catcaatccc gtttattttg gtggagcaga ctgcatcaaa
    23341 atcgtcgctg gtttttttct caatcagctt atctgccaaa acacctgcac catgaataat
    23401 tccagtcacc ggaccaattt ttttaacaac cggagcaatt gccgttttca acttttttgc
    23461 atcagttaca tctgcactga catattccgc atttccgccg gcattgctga tgcgttccaa
    23521 atttttccgg atgtcccggc ccgcttccac ttttccaacc agttgattta cttttgcagg
    23581 agtcggtttt tctccagaat caaccagtga cttcatggcc gcctgcttga gttttgtctt
    23641 atcttcatca actcctttgg cccattccgg ttcatcttca atggtagagc gacccagcag
    23701 gatgaaactg cagggctgcg tttgagcgag tttaacaaca cattctgctg taactcctct
    23761 tgcaccgcca ctgaccaaaa atacagattt ttttgttaaa gatttactgc tgtttttagt
    23821 tgttaaatct cgaataattt ttggttgaac tgtcattcgc ctggtaccgg actttcctga
    23881 tccggaatag ccaacctcct tgatccgcaa atccggatcg agaagttcct gtaaaataca
    23941 gcttgctgca gtatctgcgg taagctccgg ctgcaaatcc acaaaacggc aaaaaacatc
    24001 cggccactct acacctgctg ttttaatcaa tcctgaaagt ccactgccaa ctgcaccaaa
    24061 ctgtccggat cccattccaa gttcaccatc aagacgggtt actgtcacaa aatggctgcg
    24121 acttgaatca gattttccgg cttcattcag gaaagtgcaa acattttttg cagtgagaaa
    24181 tgcctgcttg agaaaagcat tggcaccatc ttccagcttt gattcagcag gtgctgaaga
    24241 agcagggtga agatgaatga atccgccgat atttccgcgt ttttctgtca gggatttgag
    24301 tgatgtttct agttcagatt ctgctgaaga agacagttca attagtggaa tttctttggc
    24361 aaagggtttt cgcttctttt ttacaagttc agaaataccc gagaaacgca aaatcacagg
    24421 tgaccatcct tgttcactta aagcttttgc cagaaccggt gccaatgaag aaccgtcgtc
    24481 tgtgatcaaa caaaggggat tttcaggaag ttccgcatgc agtacatccg gtggagccag
    24541 cggtaccaca tgagctaaac caagggtgac ttgatgctct ggaactgcga attcagattt
    24601 tgggttcagg agtttttttt tacagagtcg gaatctgttg aaacaggtgc aggaataata
    24661 gttgttcctg cagtctctga tggagcaagt gaactcaaat gatcaacaat ttccttcagt
    24721 gtccgcagtt cactcatttc actaccgctg acctgcggca aatgaggaag ctgttcctga
    24781 agcgaccaca taatttcgac acgcttaatt gaatcaatac caaggtctgc ttccatgtcc
    24841 ataccaagtt caagcatttc tgcaggataa cccgtttttt caccgatgat agccaagaga
    24901 gcgggagcta tctcaccggg aacggggtct gatgaaactg ccccactgcc gtttccattt
    24961 ccgcctggag aacctgcact taccgataaa ggtgccggct cgagatcaag atttgatttt
    25021 tgaacgacat cttttccacc gaacgcaggt aaaagtgatt tgatgtgctc gataatttga
    25081 ccgaccgtcc taagttccgc aacttcgttt gcaccaatct gcggtaaatc ctcaaattgt
    25141 tcctgcagcg accacattat ttcaacacgc ttgattgaat ctatcccaag gtctgcttcc
    25201 atgtccattc caagctccag catttctgca ggataaccgg ttttttcact aataattgac
    25261 ataaaaaccg taccaggatc ctgaccagtg tctggtgttg gattttgagt gtttagttct
    25321 gaatctggaa ttgcgacagt agacgggatt tctcttgaag cttcagaaac atctctgcca
    25381 attttactct ttaagtgatc aataatttga ccaagtgtac gcatttcagc aagttcatct
    25441 cctgggactt ccggcaattc aggaatctta tccttaaccg ccccaagaat ttctacacgt
    25501 ttgattgaat caatgccaag gtctgactcc atgtccattc caggttccag catctccagt
    25561 ggatagcccg ttttttcgcc gacaatagcc atcattgccg cagtgatatc ttctgcggaa
    25621 actcccgagc ttgaagccgc agatgcagca gcagtaattt cacttgtaag cgtgcctgca
    25681 gtttgtaggg cagaatcatc tgtacccaat ttgctctgca gatgcgcaac aatttcgcca
    25741 agggtcctca tttcggccaa atcatcacca ggaacttccg gcaatgcagg aattttat
//
LOCUS       DTVD01000150            7760 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2683_length_7760_cov_343.372485, whole genome
            shotgun sequence.
ACCESSION   DTVD01000150 DTVD01000000
VERSION     DTVD01000150.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_7760_cov_343.372485"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="EYO68_05355"
     CDS             <1..877
                     /locus_tag="EYO68_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC07267.1"
                     /translation="CLRCSDTRVFISVRPRERYAHARLCDCVSSPCKTCKGTGFLVEQ
                     DSFQRDVAITCPDCEQIKKRVQLYNSARIPRRYLNSRLNSQDRDSGNEMVFDLLESIF
                     RLLPQRLSNKNNLQNDTEDLKGMVLMGPPGTGKTHLMTGFVYQCTIGHGISCIFQSFA
                     ELLSELRQGYSDGKSDMEIIEPHLQTDILIIDDMGKGRNSDWELGILDMLISERYNRN
                     QIIMVTTNFTESEEHTLKEQIRSRDKSETEQFITDTISRRVGERIHSRLKEMCYFENL
                     MGQDRRITEDELDDE"
     gene            complement(878..1906)
                     /locus_tag="EYO68_05360"
     CDS             complement(878..1906)
                     /locus_tag="EYO68_05360"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIC07268.1"
                     /translation="MVRIGIIGAAGLSGRELLYLLQSHDEASVELLTSSKYQGKKVND
                     VFPELTGYNQVFQPNDIDVTGCDLVFLAIPNQASLERVPNLLEQGVRVIDLSGAFRLR
                     DKEVFSKFYKLSHSAPELLEEAVFGLPEYFKSEITTARLVANPGCYATGALLGLLPLG
                     ELLGKLDRPPIIDAKSGVSGAGGRVEDDVTNFVSVNENFKAYKVFSHQHQPEIQQYLQ
                     DLSPYSSDVTGEIILTPHLLPLSRGILSTIYLHFREPVTSSDILERFSTFAESQEFVH
                     FLEEGDLPDLRMTVHSNRCMIGAETDQSGQNCVIVSSIDNLVKGASGQAIQNMNLMFG
                     LDEKQGLL"
     gene            complement(1907..2101)
                     /locus_tag="EYO68_05365"
     CDS             complement(1907..2101)
                     /locus_tag="EYO68_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07269.1"
                     /translation="MESASLILTGKGKKRQEWNPASDDKANILKDVIGPSGNLRAPTW
                     RIGNEFIVGFNPELYEEVFG"
     gene            complement(2368..3147)
                     /locus_tag="EYO68_05370"
     CDS             complement(2368..3147)
                     /locus_tag="EYO68_05370"
                     /inference="COORDINATES: protein motif:HMM:PF08241.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC07270.1"
                     /translation="MRLKAKFHKNPKAFKDNFTTQSKAYAAYRPLYPETLFRYLAGEC
                     REHKLAWDAGTGNGQSAQKLALYFDQVYATDASSTQILNAFPLENISYAVANEQAPAL
                     KRRSVNLITVAQALHWFDTEVFYAEAERVLKRYGVLACWSYKLFRINPDVDREVDCLY
                     KDILGNYWDPERRLVDTGYRTLSFPFREFRAPKIELMSTWDFENMLGFLSSWSAVANY
                     KKRKGSDPIAVILDRLKAVWGEPVEKKNVKWPLSIRVGRIR"
     gene            complement(3200..4777)
                     /locus_tag="EYO68_05375"
     CDS             complement(3200..4777)
                     /locus_tag="EYO68_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate monoester hydrolase"
                     /protein_id="HIC07271.1"
                     /translation="MRKNILFIMCDQLRADYLSCYGHPFLETPNIDRLAERGVRFSNA
                     YCQAPLCGPSRASFYTGRYLASHGALVNADPLKLGELSLGDYLQKINYRTVLVGKSEA
                     RANQDALARLQIDQRSNLGQRLAQGGFEHYEHFAGIYPDEIVPDDLAYNDYLRSNGYD
                     GENLWESWANSAYDVNGKKVSGWQMRNANLPAAVAEEHSETAFTTDRALEFLSGVDPN
                     DQWCLHLSYIKPHWPYLVPAPYHSLYCKDQILPVVRNPDELQAPHPVFKAFHEQTYSQ
                     NFSRDEVRERVVPTYMGLIKQIDDHLGRVFEMLKSKKLMDSTLIVLTADHGDYLGDHW
                     LGEKDLFHEPSVRIPLIVCDPETYAESTRGTVNHEFVEAVDIVPTMVEFAGGNICKQR
                     MEGQSLLPLTRGILDPDNKREYTISQIDYSDRGPRVSLNLHPYDCRAHMIRTKDWKYI
                     LHEKFSPQLFDLKNDPEEFYDLGADPEYQAVGKEMHEQLFTWFRERLIRTEMEHDFLF
                     EMGVERDEKMGILIGHW"
     gene            complement(4814..5521)
                     /locus_tag="EYO68_05380"
     CDS             complement(4814..5521)
                     /locus_tag="EYO68_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIC07272.1"
                     /translation="MKLGILDTVPRNYWSTDLGITDSEKFIDLLTPVMPDATLDQLFV
                     AENEWPESLYSYDAYLITGSPCSVNEGHLWIEKLQQFVRDCLVSQKKLVGICFGHQLI
                     ASALGGTVERRRDGWLLGAESFDIVDVQSWMKPTQMKCEIFHINQDHVSVLPENAQLI
                     GHSELCENSAFVIGDSVLALQGHPEQPRRAMENFIKELLLLGANPQEMEYGRSRLRDE
                     VPDANLWARWICEFLQN"
     gene            6019..7539
                     /locus_tag="EYO68_05385"
     CDS             6019..7539
                     /locus_tag="EYO68_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306846.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HIC07273.1"
                     /translation="MYDTNFLAQHLRAASLGRDNSTFIRLHETDNTISFQDFFQNAER
                     IASVLINSGIQPGDRVAVQVEKSVSALELYMGTVLAGSIFLPLNPAYTPAEIEYFLRD
                     ARPALFVCDPSKIEMLTTVAETCGVTQVWTLTDENTGSLVEARNSESPGFRGIERGPD
                     DLAAILYTSGTTGRSKGAMLTHRCLHSNAEALVDTWRFGANDVLIHALPIFHTHGLFV
                     ATNVSLLSGSSMIFMHKFDTDGICQRMTEASVLMGVPTFFHRLLQNQELEQASKNMRL
                     FVSGSAPLLAQTHVLWRERTGHAILERYGMTETNMNTSNPYDGERRAGTVGFPLPGVE
                     VIVTNPENGQILPSGEIGVLEVRGPNVFKGYWEMPEKTSEELRENGFFITGDLGTISE
                     DGYVSIVGRSKDLIISGGYNIYPKEIEVLLDGMDGVLESAVIGVPHPDFGEGVVAVIV
                     PQSGAKLIQEDIMLKLKDQIARFKHPKRIVFIDELPRNTMGKVQKNDLRERFARLF"
BASE COUNT         2164 a         1828 c         1587 g         2181 t
ORIGIN      
        1 ctgcctgcgt tgcagcgaca ccagagtttt tatcagcgtt cgtccacgcg aacgatatgc
       61 tcacgccaga ctttgtgact gcgtttcaag tccctgcaaa acctgcaaag gtacaggttt
      121 cctcgtagag caggacagtt tccaacgtga tgttgctata acatgcccgg actgcgaaca
      181 aatcaaaaaa cgcgttcagc tctataacag tgcccgcatc ccccgtcgct acctgaacag
      241 ccgactgaat tcgcaagatc gagattcagg aaacgagatg gtatttgatt tacttgaaag
      301 cattttccgg cttctgccgc agcgattgag caataaaaat aatttgcaaa acgacacgga
      361 agacctgaaa ggcatggttc ttatgggccc tcccggtacc ggaaaaacac acctaatgac
      421 aggttttgtc tatcaatgta ctattggtca tggtatatcc tgcatttttc aaagttttgc
      481 agagttgctt tcagaattaa gacagggtta ttccgacgga aaatcagata tggaaattat
      541 tgagcctcat ttgcagactg atatcctcat catcgacgat atgggcaaag gacgaaacag
      601 tgactgggaa ctgggcattc tcgatatgct gatttctgag cgctacaacc gcaatcaaat
      661 catcatggtt accactaatt ttactgaaag cgaagaacac acacttaagg aacaaatccg
      721 tagtcgtgat aaatctgaaa cagaacaatt cattactgac accataagca gacgagtggg
      781 tgagcgtatt cactcccgtt tgaaggaaat gtgctatttt gaaaatttga tggggcagga
      841 ccgtcgaatt actgaagatg aactcgacga tgaatgatca aagcaagccc tgcttttcat
      901 ccaaaccaaa catcagattc atattttgaa ttgcctgccc tgaagcaccc ttgaccaaat
      961 tatcgatgct gctgacaatc acacaattct gtccggactg atcagtctct gctccaatca
     1021 tgcagcggtt agaatgtaca gtcatcctta aatctggcaa atctccttcc tccaaaaagt
     1081 gaacaaattc ctgactctca gcaaatgttg aaaaacgttc caaaatatca cttgaagtaa
     1141 caggttcgcg gaaatggagg taaatcgtgg ataaaattcc tcggctaagc ggaagtaaat
     1201 gcggtgtcag gataatttct cccgtgacat ccgagctata cgggctgagg tcttgcaaat
     1261 actgctgaat ttcaggctgg tgctgatgtg aaaaaacttt gtaggctttg aaattttcat
     1321 tgacacttac aaagttcgtc acgtcatctt caaccctgcc tcctgcacca ctcacccctg
     1381 attttgcatc aattatcggc ggccgatcta attttccaag taactctccc aacggcaaaa
     1441 gccccagcaa ggcacctgta gcataacatc ctggatttgc aaccagtctt gctgtggtaa
     1501 tttcgctttt aaaatattcc ggtagtccga atacggcttc ttccagcaac tccggggcag
     1561 aatgtgatag cttataaaat ttggaaaaaa cctctttgtc ccgtaaacga aatgctccgg
     1621 aaagatcaat cacccgtaca ccctgctcca ataagtttgg aacccgttcc aagctggcct
     1681 ggttggggat tgcaagaaaa acaagatcgc atcccgtaac gtcaatatcg ttcggctgaa
     1741 aaacctgatt gtaaccggtc aactccggaa aaacatcatt gactttcttc ccttgatact
     1801 tactggaagt cagtagttca acagacgcct catcatgact ttgcagcaaa tacaggagtt
     1861 cacgtccgga taaaccggct gctccaatta ttccaattct taccatttag ccaaagacct
     1921 cttcataaag ttcaggatta aagcccacaa taaattcatt gccaattctc caggttggag
     1981 cacgcaggtt tccactcgga ccaattacat ctttcaaaat atttgctttg tcatcagaag
     2041 caggattcca ctcctgcctt tttttacctt taccggttaa aataagactt gcagactcca
     2101 tcagtttcca ggcttcctgg ctgtttatct tttctttgtt ggcaaaaaca cgtgtttcta
     2161 ctgaaatatt ttttgcgtca agaacctctt gcgctctctt acaggatgtt caaccattcc
     2221 tgaaataacc ccaatttatt tgtttgctca ttttcccttt cagatattgt gttttttcaa
     2281 atcattatcg tgtttaaagt ctagctgatg cgggtctcac aatcaaatcc ttcccattat
     2341 ttgccacaga attaacaaac atgattttca gcggatgcgg ccaacacgaa ttgaaagcgg
     2401 ccactttaca tttttctttt caacaggttc accccaaact gcttttaaac gatccaatat
     2461 aactgcaatc ggatcacttc ccttgcgttt tttataattt gccaccgcag accaactgct
     2521 gagaaaaccg agcatatttt caaaatccca tgtcgacatt aattcaattt ttggagcacg
     2581 aaattcacga aatggaaaag acaatgttcg gtagccggta tcaaccaaac gacgttccgg
     2641 atcccagtaa ttgcctaaaa tatctttata caaacaatcc acttcacggt caacatccgg
     2701 atttatcctg aataatttgt aggaccagca ggcaagcacg ccatatcttt ttaggacccg
     2761 ctcggcttct gcgtaaaaaa cttcagtatc aaaccagtga agagcctgcg ccacagtaat
     2821 taaattaaca ctacgtcgct tgagtgcagg agcctgttcg ttggcaacag cataactgat
     2881 gttttccaga ggaaaagcat ttagaatttg ggtggagctt gcatccgtgg cataaacctg
     2941 atcaaagtac aaagcaagtt tttgtgcgga ctgaccattt cctgtcccag cgtcccatgc
     3001 cagtttgtgt tcccggcatt caccagccag gtagcgaaaa agggtttcag gatacaaagg
     3061 acgataagcg gcatatgctt tggattgcgt tgtaaaattg tctttgaatg cttttggatt
     3121 tttatgaaac ttggccttta atctcatttt agaacgaata actttgggtt ggtaaagtaa
     3181 gcatattttt tcatccctct caccagtgtc cgatcaaaat tcccatcttt tcatccctct
     3241 caacccccat ttcaaacaga aaatcatgtt ccatttctgt acgaatcaag cgctccctga
     3301 accaggtaaa tagctgttcg tgcatttcct tgcccacagc ttgatattcc ggatcggctc
     3361 ctaaatcata aaattcttcc ggatcatttt tcagatcaaa aagttgcgga ctaaattttt
     3421 catgcaaaat gtatttccag tccttggtgc gaatcatgtg agcgcggcaa tcgtaaggat
     3481 gcaaattcaa cgaaacacgg ggacctcgat cgctgtaatc aatttggctg atagtgtatt
     3541 cccgtttatt atccggatct aaaattccgc gtgtcagggg aagcagtgat tgcccttcca
     3601 tgcgctgttt gcatatattg ccaccggcaa attcaaccat ggtcggcaca atgtcaacag
     3661 cctccacaaa ttcgtgattc actgtgcccc ttgtcgattc tgcataagtt tccggatcac
     3721 aaacaattaa cggaattctg acagacggtt cgtgaaacaa gtctttctcc cccaaccagt
     3781 gatcacccaa ataatcccca tgatctgctg tcaaaacaat caaagttgag tccattaatt
     3841 ttttggattt cagcatttca aaaacacggc ccaaatgatc atcaatttgt ttgatcagtc
     3901 ccatataggt cggaaccaca cgttcccgga cttcatcgcg tgaaaaattt tggctgtaag
     3961 tttgttcatg gaatgcttta aataccggat gcggagcttg aagttcgtcc ggattacgaa
     4021 caacaggcaa aatctgatct ttacagtaaa gcgaatggta cggagccgga acgagatacg
     4081 gccagtgcgg tttgatgtaa ctaagatgca aacaccattg atcatttggg tctactccgg
     4141 acaaaaattc cagagcgcgg tctgtggtaa atgcagtttc ggaatgttct tcagcgacgg
     4201 ctgccggcag atttgcgttt cgcatttgcc aaccactgac ttttttgccg ttgacatcat
     4261 aagcactgtt ggcccaactt tcccaaagat tttcaccgtc atatccgttg gaacgcaaat
     4321 aatcattgta agctaaatca tcgggaacaa tctcgtccgg atatattcca gcaaaatgtt
     4381 cataatgttc aaatcctcct tgcgccagcc gttgaccaag atttgagcgt tggtctattt
     4441 gcaagcgtgc cagtgcatcc tgattggctc tagcttcaga ctttccaacc agaacagtac
     4501 gataattgat tttttgaaga taatcgccca aagaaagttc ccctagtttt agcgggtctg
     4561 cgttgaccaa agctccgtgt gaagcaaggt aacgtccggt gtaaaaactg gctcgcgaag
     4621 gaccgcagag cggggcctgg caataggcat tcgaaaaacg tactccccgc tcggcaagtc
     4681 ggtcgatgtt gggcgtttca agaaacggat gtccatagca ggagagataa tccgcccgta
     4741 attggtcaca catgatgaat aaaatgtttt ttcgcatatc ctactcgtta aaaaattctg
     4801 ctgtgttttt gaatcaattt tgcaaaaatt cacagatcca gcgtgcccat aaatttgcat
     4861 cggggacttc atcccggaga cgggagcgtc cgtattccat ttcttgtgga tttgctccga
     4921 gaagcaaaag ttctttgatg aaattttcca ttgctctgcg aggttgttcc ggatgacctt
     4981 gcagtgccaa caccgaatcc ccaatcacaa aagcactatt ttcacaaagt tcggaatgcc
     5041 cgatgagttg ggcgttttcc ggaagaacgg aaacatgatc ttggttgata tggaaaattt
     5101 cacatttcat ttgggtgggc ttcatccatg attgtacgtc cacaatatca aatgattccg
     5161 ctcctaaaag ccaaccgtct ctgcgtcgtt caaccgttcc tccaagagcg ctcgcaatca
     5221 attgatggcc aaaacagatc ccgactaatt ttttttgtga aaccagacaa tccctgacaa
     5281 actgctgtaa tttttcaatc cacaaatgtc cttcattgac actgcaaggg ctgcctgtaa
     5341 tgaggtaagc atcataagag tataatgatt ccggccattc attttcggcc acaaaaagtt
     5401 gatccaatgt cgcatctggc atcacgggag tcagcaagtc tataaatttt tcggagtccg
     5461 taattcccag atccgtagac cagtagtttc gaggaaccgt atccaatatt ccaagtttca
     5521 tttgttttcc attcctaagt aaacaggcga ttcatccgga agaaggacgt aatgcatcaa
     5581 acagtacttt gagcaatcag cgctatcccg taacgagact gtatttcagg aacaatggct
     5641 accagcatcc cttctacaat tccgttcaaa gctacaaccg ataaaatact gctatccact
     5701 ttccttcaat tcgttcttct aacataaaaa acctcgttta attttactat ttagatcagt
     5761 aaaactactt gatgttccat catttatcaa cttcaataaa ataatcattg aaattattac
     5821 aaaactaatt tattttctat ctgtttttga tattcgtttt atacagaatt ttaactaacc
     5881 atatcctgtt tatcattttc agccttatga acaatcgacc ttacacctca tctaggtaga
     5941 ttaaagaagt cgaaccgctt tgggcggagt gcttataccg cttgatccgc ttgggacgca
     6001 tctataaaaa acccgtttat gtacgacacc aattttctcg cacaacatct acgcgccgcc
     6061 tcgcttggca gggataattc taccttcatt cgtttgcatg aaacagacaa cacgatcagt
     6121 ttccaagact tttttcaaaa cgctgaacgc atcgcctctg tactgatcaa ctccggaatt
     6181 caacccggag acagggtggc ggtgcaagtc gaaaaatcgg ttagcgcact cgaactttat
     6241 atgggaacag ttttggccgg atcaattttt cttcccttaa atccagctta tactcctgca
     6301 gaaattgaat attttctgcg tgacgcacgc ccagcccttt ttgtttgtga tccttcaaaa
     6361 atagagatgc taaccactgt cgccgagacc tgcggagtta cgcaagtctg gacactaact
     6421 gacgaaaata cggggtctct ggtcgaggca cgaaattccg aatctcccgg attcagagga
     6481 atcgaaaggg gaccggatga tttagctgcc attctgtaca cctccggaac tactggccgc
     6541 tccaagggag cgatgctgac acatcggtgc ctccattcaa acgcggaagc actcgtggac
     6601 acctggcgtt tcggcgcgaa tgatgtgctg attcacgctc tgccgatatt tcacactcac
     6661 ggtttatttg ttgccaccaa tgtttcccta ctgtccggtt cttccatgat ttttatgcac
     6721 aaatttgaca cagacggaat ttgtcagcgc atgacagagg ccagtgtttt aatgggtgtt
     6781 ccgacctttt ttcaccgtct tttgcaaaat caggaactgg aacaagcctc aaaaaatatg
     6841 aggcttttcg tttccggttc ggcaccgctg ctggcccaaa ctcatgtgct ctggcgtgag
     6901 cgcactggac acgcgattct ggagcgctac ggcatgaccg aaaccaacat gaacacttcc
     6961 aatccctacg acggtgaacg gcgggccggt actgtcggtt ttccgcttcc cggagttgag
     7021 gtgattgtta ctaacccgga aaacggacaa attttgccgt ccggagaaat tggagtgctt
     7081 gaagttcgtg ggccgaatgt gttcaaaggc tattgggaaa tgccggaaaa aacctccgaa
     7141 gagttgcgcg aaaacggttt ctttatcacc ggtgatcttg gtacaatcag cgaagacggt
     7201 tatgtttcca ttgttggacg ctcaaaagat ttaattattt caggaggtta taacatatat
     7261 ccaaaagaaa tcgaggtttt gcttgatggg atggacggag tattggaatc ggcggttatc
     7321 ggtgtgccgc atccagattt tggtgaaggt gtagtggctg ttattgtccc tcaatccgga
     7381 gcaaaactaa ttcaagaaga tatcatgttg aaactgaaag accaaatcgc ccgtttcaag
     7441 catcccaagc ggattgtttt tatcgacgaa cttccgcgca acacgatggg gaaggtacaa
     7501 aaaaacgatc tgagagaacg ttttgcaagg ttgttctgat tattttgaac aacttgtaga
     7561 tccggtgggt tctaaataca tggaagcttt ttggtgattc attgattcag gtttgcatca
     7621 tcctgcaatt ctgagaggat ggaacgcccc atcgccgtgt ctgccaaggg acgagtcaag
     7681 tctcccaagg tatcaagttg cgtcttgaga gcttgagaat cctgcccctt catgctttgt
     7741 cgtactacat ccatttgctt
//
LOCUS       DTVD01000151            1914 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_27024_length_1914_cov_473.976869, whole genome
            shotgun sequence.
ACCESSION   DTVD01000151 DTVD01000000
VERSION     DTVD01000151.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27024_length_1914_cov_473.976869"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            513..830
                     /locus_tag="EYO68_05390"
     CDS             513..830
                     /locus_tag="EYO68_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07274.1"
                     /translation="MSYVIHVTAVVEGDLDQIEILMNDYRCKCLENQIGMEQFFVCRS
                     VEQKNVFLYTQVFTDQAAHKVHLEGEDPKWFFEQMSENKFEFQGQWVAGTEIDSSAGH
                     VLN"
     gene            1142..>1914
                     /locus_tag="EYO68_05395"
     CDS             1142..>1914
                     /locus_tag="EYO68_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail-specific protease"
                     /protein_id="HIC07275.1"
                     /translation="MKVSKYLLLRLALLLFLFPSFLAAQDEKQKLTGPVVKGIVEQFV
                     SMHYAQKPLDDEMSAKIFKLYLNRLDPAHYYFLAADVNEFQKYETRIDDMLLRGKVKL
                     ALEIFERFKVRLSERLAMMEEFLAEDFDFSRDANWTLERDDLPYPETTEEARKIWRTK
                     IKFDLLTLKLADTSIKDGKERLMKRVRGMWKDFSQYENDDVVSIFLNSMAAAYDPHSS
                     YLAAQDLKNFDISIKLSLEGIGAVLRWEDGYTVVNSIVPG"
BASE COUNT          581 a          329 c          411 g          593 t
ORIGIN      
        1 aatgtggaat tttacttgga aagttttgga aggaagagta taaatgtggt atggggattt
       61 taaaatttaa ttgtcccata ttttaaacaa ctaatgaaag gaggtgtgaa tgtctagtca
      121 gataatgatg gtgattcttc ccctttatag tttggtgagg aatcaccttt gatcaaaaag
      181 tttcattttc aggtaaggaa aattgtttga aaatgatggt ataaccatct cataaataat
      241 tacatccttt cttaatttgg aaggttattg ggatgatgtt tgaggtgtga attctcattc
      301 atcatccttc ttattagcgg ctaatcttca ctgcttgctc aatcttcatt tgaaaattta
      361 atccaacagg ggcacttagt tgtgtgcggc cgacaggaca taaaaatctg ttaactgcac
      421 aaaaaaagca gtttaaataa ttaagtattg tgtcggcctg gttaataaga ataaaatagt
      481 actgtaatta taaccttttt agattaatac aaatgagtta tgtgatacat gtgacggcag
      541 ttgttgaggg agatcttgat caaattgaga ttttaatgaa tgattaccgt tgcaaatgtc
      601 tggaaaacca gattggaatg gaacagtttt ttgtttgccg gagtgtggag cagaaaaatg
      661 tttttctcta cacgcaggta tttacagatc aggcagcaca caaagtgcat ctcgaagggg
      721 aggatcctaa gtggttcttt gaacagatgt ccgaaaataa attcgaattt caaggacaat
      781 gggttgcagg gactgaaatt gactcctctg caggacatgt cctcaactga caaatatgga
      841 agggagatag aaaataagag gaattgtaac ctcgtttcat tggaagaata atcatctccc
      901 ttccactaaa ttccgtaatg caactgcaga acttaattaa tatgccgcag aagtacattc
      961 actttaatga gtgaattccc actgctctcc acctgatttt cacttcctgt tttgtttaaa
     1021 taattcttca taccaagttc gaatctggaa gaagttcggc aaattgattg atatttcttg
     1081 taattcatga cataaaagta gttcgtttta aactataatt tacataaaaa caaaaaaata
     1141 catgaaggtt tccaaatacc ttttgctccg cctggcactc cttttattcc tttttccgtc
     1201 ctttttagca gcacaagatg aaaagcagaa gctcaccggc cctgtcgtca agggtatagt
     1261 tgagcagttt gtctccatgc attatgctca aaaaccgctg gacgatgaga tgtctgcaaa
     1321 gatctttaag ctttacctga accggctgga tccggcacat tattattttc ttgcagcaga
     1381 cgtgaatgaa ttccaaaaat acgaaacacg tattgacgat atgctgctgc gcggaaaagt
     1441 aaaattggct ttggaaattt ttgaacgctt caaagtgcgt ttgtcagagc gcctggcgat
     1501 gatggaagaa tttcttgctg aggattttga tttcagccgc gatgcaaact ggacattgga
     1561 gcgcgatgat ctgccttatc cagaaacaac agaagaggcc aggaaaattt ggcgtaccaa
     1621 aatcaagttt gatttgttga ccctgaagct tgctgatacc agtatcaaag atggaaaaga
     1681 gcgcttgatg aagcgtgtac gtggaatgtg gaaagatttt tctcagtatg aaaatgatga
     1741 tgttgtttcg atattcctca attcaatggc ggcagcttat gatcctcact cttcctatct
     1801 ggcggcacag gacctgaaga attttgacat ctcgattaaa ctctctcttg aaggcatcgg
     1861 tgcggttttg cgttgggagg acggctacac ggttgtaaat tcgattgttc ccgg
//
LOCUS       DTVD01000152            7714 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2717_length_7714_cov_273.729860, whole genome
            shotgun sequence.
ACCESSION   DTVD01000152 DTVD01000000
VERSION     DTVD01000152.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_7714_cov_273.729860"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(2..3472)
                     /locus_tag="EYO68_05400"
     CDS             complement(2..3472)
                     /locus_tag="EYO68_05400"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03572.16,HMM:PF07676.10,HMM:PF14685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HIC07276.1"
                     /translation="MKSKTKFSKKLLPTLNGTGQGYFTQPTISNKQIIFVCENDLWQV
                     PLEGGRAQRLTSSRSETHSPVYSPNGQWIACCTMEEGEHDVYLMESSGGPLQRLTWLN
                     SVTHIVSWSHDGQYIWFRSTHQAVHSRGSDAWIFRVSINGGPVERLPYGPAMTVDLQV
                     NGKKGVVLGRNTLSNSRWKRYRGGMAGEIWVDKNNSGNFKRLFRDLQGNPVSPFWLRE
                     RLWFVSDHEGVGNLFSCTPEGGRLRQETFQKEYYVRFPATDGKALIYHVGGELWTLDP
                     EENSGPERETQIPIGWHSTKTRLQRRFFYGDEYWEETMIHPQGHEVALTARGKLFSMP
                     FWEQAVRQHGIRDGVRYRMPCWLPNGKLAAISDAPLKKNKTKKLSAMEEQLDVFGSTP
                     TEFPDCSHRLPPGRMQEIAVSPRFQHLALTTSRMELFLINAETGRISKLDDSKIREIS
                     DPVFSPDGRWLAYTKHLSLELTAIFLLDLKPSANGKRIKAKDPVQITQPVRYDFSPAF
                     DPEGRWLYFLSSRIYNPIWDTVQTGTSFSRSMKPYLLTLKKDSPNPFEALPHAPGGQE
                     TGGTPAGDSSHETQDSNNSTTKQSKDSKKVALEIDLDGIAERIVEFPVSEGVYKQIIG
                     LPNKVIFTEFPLSGALHDLEAEDNQDKDEGILWVYDFEKQEAETIAENVGIVQTSVPD
                     ESENSSRTMLYSSGDLLRALEAGVSVPEDTKTEKQHSRKSGWLDLRRIRISVQYQEEW
                     AQMFQETWRLQKEFFWTEDLSGVDWERVYQRYARLLPRIGSRSELSDLIWEMQGELGT
                     SHAYEYGGDYPYSPRYPVGCLGADLVFDSKQKSWIFQKIYSGDIWKPNEHSPLAEPGV
                     SVEEGERLLAVGGVPVDEHKTPGELLVHQAGQIVPLSVGQGTSKKKQVKGPKGQVSSV
                     KNFEDDARQVLVKTLSGEQEVRYREWVRENIKTVDSLTGGRVGYLHIPDMSTHGIAEF
                     HRGYLAQVDREGLIVDARYNAGGMVSPLILEKLAHRHLGFDVPRWGTPESYPYHTLRG
                     HLIVIANQFTGSDGDMFTTSFRQLQLGQLVGKRTWGGVIGIDGRYQLVDGTTTTQPQY
                     SIWFHHAGWSVENHGVDPDIEVEDTPQSYINNEDPQLERTVKEMLRLLKEKPVLSVNY
                     SPSPRRLLPE"
     gene            complement(3649..5481)
                     /locus_tag="EYO68_05405"
     CDS             complement(3649..5481)
                     /locus_tag="EYO68_05405"
                     /inference="COORDINATES: protein motif:HMM:PF05134.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07277.1"
                     /translation="MDNVFYLVSAHTSCIEGIRLSIKNKHVYITQDIVGFSADEEEDS
                     AGKNGSDSEQWAAKIGKFLDGQQWSDHAIAFLLPAEDVTFRKLSFPFQERKKVEQALP
                     FELEEELMGDLAETAYSVQIQSTTEQHSEALVLLIGRKRLQQLQQLCLERDLIIRNVD
                     CAAHALYRSKIFDNSTTIETQDLFQIYIGSDESFVNTIHEKRLDEIKIFPNQIPVILQ
                     KHFSTENLLAAFLQSFAKHPDGVDNADGDSAQNEAFIQLKEELRWLCAQLTLYLRIKN
                     FSSESQIEVYGIFGPMIKWDGLKFSIRSFPLPEAEAFAERSVEDPDFIGTSEESVKDG
                     ILTISQTDVKAPDTLEELLVEAKQRERSDEKTQTSDDQITTTPSEEKKKNRPSSGVLE
                     SINPQTSLLSLIERKHWGVLGELRKKTEIILESHRLSLYYENTPWRRILLRNRVAFAV
                     AASLIIIISAGFVWNTITQLDLLHQEIVRGERLVQTELRQVLPNTSASGVNAMLMELE
                     EKIELRKAYIKNSKSFEKRDYHNLSFLKNISALLSEDAPFQVDSLEYAPERFSISGTI
                     DSYDSLQILKNNLQEIKEFKGRRIVESNRKSPDGIVFRISVDFK"
     gene            5802..5993
                     /locus_tag="EYO68_05410"
     CDS             5802..5993
                     /locus_tag="EYO68_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07278.1"
                     /translation="MAKIELEVGTCPTGVLLALKSVEGRVHHVTAIEMTNDEALEISK
                     LIKQRVKENLESPEPSEIN"
     gene            6016..7350
                     /locus_tag="EYO68_05415"
     CDS             6016..7350
                     /locus_tag="EYO68_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07279.1"
                     /translation="MNKERKSVWGWAMYDWANSAFATTVMAGFFPIFFKQYWSIGTDV
                     NQSTAILGFGNSIASLLVALMAPVLGAIADRGSLKKKFLIFFAYLGVLMTAGLYMVEK
                     GDWMMAVFVYVMGVIGFSGANIFYDSLLPSVADESKIDSVSSLGFAMGYLGGGLLFLV
                     NVVMTLQPAIFGLTDAGEAVRWSFVSVALWWGVFTLITIFWVPEPEMLGEKDQENPVL
                     GGFQQLFGTFQEIRHLKTVMLFLLAYWFYIDGVDTIIKMAVDYGMSIGFESNDLIIAL
                     LIVQFVGFPAALIFGSLGERWGVRSSIFLAIGVYIVVTIWATMMREKYEFYVLAVAIG
                     LVQGGIQALSRSYYSRLIPKNQAAEYYGFFNMLGKFAAIIGPALMGVVGLTMRNMLMP
                     DSPSAEQIKAVGQEASRWSIASIVILFVIGAVLLFYVDDEKGRAEAEYLFKN"
     gene            complement(7383..>7714)
                     /locus_tag="EYO68_05420"
     CDS             complement(7383..>7714)
                     /locus_tag="EYO68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIC07280.1"
                     /translation="FNAPHRNMVDFAHLPIVKRLTRMPVCIDPSHSVGTREASPDGVL
                     DVLHSTSQGIIAGANMVLVDFHPSPTDALVDGPQALTLDELPKFIEDTRISREAYKQR
                     VELWAEK"
BASE COUNT         2082 a         1693 c         1626 g         2313 t
ORIGIN      
        1 attactccgg aagcaggcgt cttggtgacg gagaataatt cacggataga acaggctttt
       61 ccttcagcag acgcaacatt tccttaactg tacgttccag ctgtggatct tcattattga
      121 tataagattg tggagtgtct tccacctcaa tatccggatc aacaccatga ttttcaactg
      181 accagcccgc atgatgaaac cagatggaat actgaggctg tgtggttgta gtgccgtcca
      241 ccagttggta acgcccatca attccgatga caccgcccca ggttcgtttt cccactaact
      301 gtccaagctg cagctgtcta aaacttgtag taaacatatc accatcagag ccggtaaact
      361 ggttggctat tacaatcaag tgtccgcgca gagtgtgata cggatatgac tccggagttc
      421 cccagcgtgg tacatcaaaa cccaaatgcc gatgcgctaa tttttccaga atgagtggag
      481 aaaccattcc tcctgcattg tagcgggcat cgacgatcaa accttcgcga tcaacttgag
      541 ccaaatatcc ccgatgaaat tcagcaattc catgcgtact catgtctgga atatgcagat
      601 aaccgacgcg ccctccggtc agagaatcaa ctgtttttat attttccctg acccactccc
      661 gataacgtac ttcctgttcg ccggaaagag tttttaccag gacctgacga gcatcgtcct
      721 caaaattttt gacacttgaa acttgtcctt tgggtccctt aacctgcttt tttttacttg
      781 tgccttgtcc tacgctcaat gggacaatct ggcctgcctg gtgaacgagt aattctccgg
      841 gagttttgtg ttcatcaacc ggaactccgc caacagcaag cagtcgttca ccttcttcaa
      901 cggaaacacc cggttctgcc aatggagaat gctcgttggg cttccaaata tcaccggagt
      961 aaatcttttg aaaaatccat gacttttgct ttgaatcaaa gacaaggtct gcacccaaac
     1021 acccgacagg ataacgtggt gaataaggat aatcaccgcc atattcatat gcgtgagatg
     1081 tccccaactc tccctgcatt tcccagatta aatcagacag ttctgaacgt gaaccgattc
     1141 tgggcaacaa acgggcatag cgttgataaa ctcgttccca gtcaactcca gaaaggtctt
     1201 ccgtccaaaa aaattctttt tgcaaacgcc atgtttcctg aaacatttgt gcccactcct
     1261 cctgatactg cactgaaatt ctgatgcgtc tcaaatcgag ccagccactt tttcgcgagt
     1321 gctgtttttc cgttttagta tcttccggaa cagaaacacc tgcctctaaa gctcgtaaca
     1381 gatctccgga actgtaaagc atggtacggg aagaattttc ggattcatcc ggaacagaag
     1441 tttgaactat gccgacattt tctgctatag tctctgcttc ctgtttttca aaatcataga
     1501 cccacaagat accctcatcc ttatcctgat tatcttctgc ttccaaatcg tggagcgcac
     1561 ccgaaagtgg aaactccgta aaaataactt tgttcggaag accaataatc tgtttgtaaa
     1621 caccttcaga aactggaaat tcgactattc tttccgcgat accgtctaaa tcaatttcaa
     1681 gagcaacttt ttttgaatct ttggattgtt tagttgttga attatttgaa tcctgagttt
     1741 cgtgactgga atcaccagca ggggttccac ctgtttcctg ccctccaggc gcatgaggta
     1801 atgcttcaaa agggtttgga gaatcttttt tcagtgtcaa cagatacggt ttcattgaac
     1861 gtgaaaatga ggtgcctgtt tgcactgtgt cccaaattgg attgtatatt ctggaagaaa
     1921 gaaaatagag ccagcgtcct tcaggatcaa aagccggact gaaatcgtaa cgtacaggtt
     1981 gggtaatttg gactggatct ttagctttga tacgtttacc gtttgccgac ggtttaagat
     2041 ccagcaaaaa aatagccgtt aactccaaac tcaaatgttt ggtgtatgcc agccatcttc
     2101 catccggtga aaaaaccgga tcactaattt cccggatttt cgagtcatca agtttgctga
     2161 ttcgtcctgt ttccgcgttg attaaaaaca gttccatccg gctggttgtc agcgccagat
     2221 gctgaaatct cggagagaca gcaatttcct gcatgcgtcc gggaggcaga cggtgtgagc
     2281 agtcaggaaa ctcagtcgga gttgagccaa atacatcgag ttgctcctcc attgcagaaa
     2341 gtttttttgt tttatttttt ttcaacggag catctgaaat tgctgccagt ttaccgttcg
     2401 gcagccagca aggcatcctg taccgaactc cgtcacgaat cccgtgctga cgcacagcct
     2461 gttcccaaaa cggcattgaa aacaatttcc cacgagcagt cagtgctact tcgtgacctt
     2521 gcggatgaat catggtttct tcccagtatt catcaccata aaaaaatcgt cgctgcaaac
     2581 gggtttttgt cgaatgccag ccaatcggaa tttgagtctc tctttcaggt ccggaatttt
     2641 cttccggatc aagtgtccat aattctccgc caacatgata aattagtgcc ttaccatctg
     2701 ttgcagggaa tcgtacatag tactcttttt gaaaagtttc ctgacgaagt ctccctccct
     2761 caggagtgca ggaaaaaaga ttaccgacac cctcgtgatc tgagacgaac cacagtcgtt
     2821 ctcttaacca aaacggactc actggatttc cctgaagatc acggaaaagg cgtttaaaat
     2881 ttcctgaatt atttttatca acccagattt ctccagccat tccaccgcga taccgtttcc
     2941 agcgggaatt actcagcgtg ttgcgaccca ggacaactcc ctttttaccg ttcacctgta
     3001 aatccacagt cattgccgga ccatacggca gacgctcaac cggaccacca ttgattgaaa
     3061 cccggaaaat ccatgcgtca ctgcctcgac tatgtacagc ctggtgagtg ctgcgaaacc
     3121 agatatattg tccgtcatga gaccagctca cgatatgcgt cacactgttt aaccaggtta
     3181 aacgctgaag cggtcctcca ctggactcca tcaaataaac gtcatgctct ccttcttcca
     3241 ttgtgcagca ggctatccac tgaccgtttg gagaatagac tggtgaatgt gtttcactgc
     3301 gggaactggt caatctttgg gctcgcccgc cttccagagg tacttgccat aaatcgtttt
     3361 cacagacaaa gataatctgt ttgtttgaaa ttgtcggctg ggtaaaatag ccctgtccgg
     3421 taccgtttag ggtgggaagt agttttttgg agaattttgt ctttgatttc acgtatggga
     3481 taaatatgta aaatgttaat gttgatgccg tcgtaacaag tcataaaacc ttgtcattac
     3541 gagtgatggc agaaatcctt tataagctac tgcactaata tcattaagat ttcttcatag
     3601 ttagaaatga ttgtgtggat ttaagactta ttgcgagatt attaatgctt atttaaaatc
     3661 aactgaaatc cggaaaacaa ttccatccgg gcttttacgg ttactttcaa cgatacgccg
     3721 ccccttaaac tctttaattt cctgcaaatt attttttaaa atttgaagac tatcatagct
     3781 gtcaatagta ccactgattg aaaaacgttc tggtgcatat tccaaactat caacttgaaa
     3841 aggcgcatct tcgcttagta atgcagaaat gtttttgaga aaactcaaat tatgatagtc
     3901 acgtttttca aaacttttac tgtttttaat ataggctttg cggagttcaa ttttttcttc
     3961 cagttccatc agcatagcat tgactcctga tgctgaagta ttgggcaata cttgtctaag
     4021 ttctgtttga accaaacgtt caccgcgtac aatttcctga tgaagaaggt ccagctgcgt
     4081 tatagtgttc cagacaaaac cggctgaaat aataattatt aagcttgcgg caactgcaaa
     4141 ggctacccgg tttctaagca aaatccgtct ccacggagtg ttttcgtaat agagactcaa
     4201 ccgatgtgat tcgagaataa tttcagtttt ttttcgtaat tctcccagta ccccccaatg
     4261 tttgcgctca atcagggaaa gtaatgatgt ctgcggatta atagactcca ggactcctga
     4321 tgatggtcga ttcttcttct tttcctcaga aggagtagtt gtaatttgat catcagaagt
     4381 ttgtgttttt tcatctgatc gttccctctg ctttgcttca accagcaact cttcaagtgt
     4441 atccggagcc ttgacgtcag tctgagaaat agttaggatt ccatctttaa ctgattcttc
     4501 tgaggtcccg ataaaatccg gatcttcaac tgatctttca gcaaatgcct ctgcttcggg
     4561 taaggggaag gaacgtatac tgaattttag accatcccat ttgatcattg gaccaaaaat
     4621 accgtaaacc tcaatttgac tctctgagct gaaatttttt attcgcaaat acaaggtaag
     4681 ttgtgcgcat aaccagcgca actcttcttt aagttgtata aaagcctcat tttgcgcaga
     4741 atctccgtca gcattatcaa caccatccgg atgttttgca aaactttgta aaaatgctgc
     4801 aagtaaattt tctgtggaaa agtgtttctg aagaatgact ggtatttgat ttggaaatat
     4861 cttgatctca tccaggcgtt tttcgtggat tgtattcaca aaagattcat cactgccaat
     4921 gtaaatctga aacaaatcct gagtttcgat agttgtagaa ttgtcaaaga tttttgagcg
     4981 gtaaagggcg tgtgcagcac aatcaacatt cctgattatc aagtcgcgct ccagacaaag
     5041 ctgctgcagt tgctgcaaac gcttacgccc aatcagcaat accagtgcct ctgaatgttg
     5101 ctcagttgtt gactgaatct ggacgctgta tgcagtttcc gccaggtctc ccattaattc
     5161 ttcctcaagc tcaaagggca aagcctgttc tacttttttt cgttcctgaa agggaaaaga
     5221 cagttttcga aatgtcacat cttctgcagg tagtaaaaaa gcaattgcat gatcgctcca
     5281 ttgctgccca tccagaaatt ttccaatctt tgctgcccat tgttcagaat cagatccatt
     5341 ttttcctgca gaatcttctt cttcatctgc agaaaaacct acgatgtcct gagtaatgta
     5401 gacatgctta tttttaattg acagacggat gccttcaata catgacgtgt gggcagatac
     5461 cagataaaag acgttatcca tgaattggct tttttcctgc tgtagagcta aggttaatca
     5521 tgtgtataac tttatcagaa tcagataaat ttctcaaaga attgaaaaat acattcgttt
     5581 cagcttgtaa ataaacacca atctggtcag aatcctctga gcatttattc ttttttaaga
     5641 tggttttgaa aaaagtcgta aatacagata ttttgaatca atctttcagg aaatagaaag
     5701 ctgatttgtt gctatttttc tgagtaaatc gtgatgagtt ttatccaatt ttgaactctt
     5761 tacgattgca tttctttata aaataattct ggagttagat cgtggccaaa atagagttgg
     5821 aagttggtac atgccccacc ggagttttgc tggctcttaa gtccgtggag ggcagagtgc
     5881 accatgttac tgcaattgaa atgaccaacg atgaagctct tgagatttca aaactcatta
     5941 aacaaagagt taaagaaaac ctggaatctc ctgagccttc tgaaatcaat taatataagc
     6001 tttcttgaga aatatatgaa caaggaacgc aaatcagtat ggggctgggc catgtacgac
     6061 tgggcaaatt cagcttttgc caccacagtt atggccggtt ttttccctat cttcttcaag
     6121 cagtactgga gcatcggtac agatgtcaat caaagtactg ctatactcgg ttttggcaac
     6181 tcaattgcca gtttgcttgt ggcccttatg gcaccagttc tcggagcaat tgcggatcgt
     6241 ggttcattga agaaaaaatt cctgatattc tttgcctacc ttggagtgct gatgacagca
     6301 ggattgtaca tggtagaaaa aggagattgg atgatggcag ttttcgtcta tgtaatggga
     6361 gtaatcggtt tttccggagc aaatattttt tacgactctc tgctgccaag tgttgcagac
     6421 gaatcaaaaa ttgactctgt ttccagttta ggttttgcca tgggatatct tggtggtggt
     6481 ctgctatttc tggttaatgt tgtcatgact ttgcagccgg ccattttcgg acttactgat
     6541 gcaggagaag ctgttcgctg gtcatttgtc agcgttgccc tgtggtgggg agtttttacg
     6601 ctgattacaa tattctgggt tccagaacca gaaatgctag gagagaagga tcaggaaaat
     6661 cctgtgcttg gaggctttca gcaacttttt ggcacctttc aggaaattcg ccatctaaaa
     6721 actgtgatgt tgtttttgct ggcctactgg ttttatattg acggtgtaga tactatcatc
     6781 aagatggcag tggattatgg tatgtcaatc ggctttgaat ccaatgacct gatcattgct
     6841 ctgttgatag tacaatttgt gggtttccca gcagcactga tttttggaag tttaggagaa
     6901 cgctggggtg tccgttcatc aatatttttg gctatcggag tttacattgt cgtgactatc
     6961 tgggccacca tgatgcgtga aaaatatgag ttttatgttt tggcagtggc gataggcttg
     7021 gtccagggag gaattcaggc tctaagtcgc tcttactact cccgtttgat cccaaaaaat
     7081 caggcagctg aatattatgg attttttaat atgcttggga aatttgccgc aattatcggc
     7141 ccggcattaa tgggcgtagt agggctgacg atgagaaaca tgctgatgcc ggactcacct
     7201 tcggcagaac aaattaaagc agtcggccag gaagcatcgc gctggagtat tgcctcaatt
     7261 gtgattttat ttgtgattgg tgccgtgctg ctgttttatg ttgatgacga aaaaggacgt
     7321 gctgaagcag aatatttatt taagaattga ttaacgctga ttaaataaca gaacagtatg
     7381 atttactttt ctgcccaaag ttctacacgc tgtttgtacg cctcccggga aattctggtg
     7441 tcttcaataa atttcggcaa ctcatccaga gtgagcgctt gcggtccatc gaccagggca
     7501 tctgtgggag aaggatgaaa atcaaccaac accatgttgg ctcccgcaat tatgccctga
     7561 gaagtggaat gtaagacgtc aagcactcca tccggagaag cctctcttgt acccactgaa
     7621 tgcgatgggt cgatgcagac cggcatacgt gtcaggcgct ttacgatagg taaatgagca
     7681 aaatcaacca tgttgcgatg cggcgcattg aatg
//
LOCUS       DTVD01000153            1902 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_27275_length_1902_cov_524.498105, whole genome
            shotgun sequence.
ACCESSION   DTVD01000153 DTVD01000000
VERSION     DTVD01000153.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27275_length_1902_cov_524.498105"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            7..1428
                     /locus_tag="EYO68_05425"
     CDS             7..1428
                     /locus_tag="EYO68_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HIC07281.1"
                     /translation="MKNILTSDRTSGILSQLSGANQSFESDYPGKSLLRQPVHTIYGG
                     AHLFKTDTAQKMGKLAIKNLNDFAPNFVTFAHALELKGAESLPKNISEINALVADAEQ
                     NGVDPANSATWLAWRVYGQVCKKMKHEAVEDFRLDYEDGFGIRPDEEEDAVAVQGAKA
                     VAAGMKQGTLPPFIGIRIKPFNSEFVERGVRTLNIFICTLLAETGGVLPDNFVVTLPK
                     VEIPEQAGALVQLFEVLEADSELSSGDLKMEIMIETTQSVFNANGEVTARKIAQAAKN
                     RCIGAHFGTYDYTASCDLIAAYQTMDSAVCDFARDVMKVAFGGSGIFLSDGATNIMPI
                     AQHRGENLSALQVRENLETVHRAWKLAYGHIRHSLAHGFYQGWDLHPGQIPVRYAANA
                     AFFLEQIQESTVRLRNFVEQASKATLSGDIFDDAATGQGLLNFFFRALNSGAIDPEDV
                     ENAGVTVEEVQAGSFRKIVEARR"
     gene            complement(1474..>1902)
                     /locus_tag="EYO68_05430"
     CDS             complement(1474..>1902)
                     /locus_tag="EYO68_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510787.1"
                     /note="high-affinity transporter for xanthine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease XanP"
                     /protein_id="HIC07282.1"
                     /translation="AAIFNTFPNTTFSQNNGVIQMTGVASRYVGMYLAGFLVILGLFP
                     VIGGFFRSLPNPVLGGATIVMFGAVAAAGVNIIASMGRLGRREFLIIAVSLGIGLGLA
                     FVPEVLSQTPKAIQQIFGSAITSGGLAALILNMVLPQNES"
BASE COUNT          531 a          405 c          473 g          493 t
ORIGIN      
        1 aaaaccatga aaaacatttt aacttcagac cggacttcgg gaatcttgag tcaactttcc
       61 ggtgcaaacc aatcttttga aagcgattat ccgggtaaat cccttttgcg acaacccgtt
      121 catacgattt atggcggagc gcatttgttc aaaaccgaca cggcacaaaa aatgggcaag
      181 ttggcaataa aaaatctaaa cgattttgca cccaattttg ttacttttgc acatgctctg
      241 gaattgaaag gagcggaaag tttgccaaaa aatatttctg aaattaatgc tttggttgcg
      301 gatgcagaac aaaatggtgt cgatccagca aactctgcaa catggctcgc ctggcgggtt
      361 tatgggcaag tttgtaaaaa aatgaaacat gaagctgtag aagattttcg gctcgattat
      421 gaggacggct ttgggattcg tccggatgaa gaggaagacg cagtggcggt tcagggagct
      481 aaagcagttg ctgcaggaat gaaacaaggg actttgccgc cgttcatcgg catccgcatc
      541 aaacccttca attcagagtt tgtggaacgc ggtgtccgga ctttgaatat ttttatctgc
      601 actttattgg cggaaactgg cggggtgctt ccggataatt ttgtggtgac tttaccaaaa
      661 gttgaaattc cggaacaagc gggggcattg gtgcagttgt ttgaggtttt ggaagccgac
      721 tctgagttgt catccggtga cctcaaaatg gaaattatga tcgaaaccac tcaatctgtt
      781 ttcaatgcaa acggcgaagt gaccgccaga aaaattgcac aagcagctaa gaaccgttgt
      841 atcggagcac atttcggaac ttatgattac acggcttctt gtgatttgat tgcggcctat
      901 caaacgatgg acagcgcagt ttgtgatttt gcaagggatg ttatgaaagt ggcctttggc
      961 ggttccggaa tttttctttc cgatggcgca accaatatca tgcccattgc tcagcatcga
     1021 ggagaaaatc tttcagcgtt gcaagttcgt gaaaatttgg aaaccgttca ccgtgcgtgg
     1081 aaactggctt acggccacat ccggcattcg ttggcacatg gattttatca aggctgggat
     1141 ttgcatccgg gacaaattcc ggtacgttat gcggccaacg ccgctttttt tctggagcaa
     1201 attcaggagt ccactgtgcg cctccgcaac tttgtagagc aagcctctaa agccactttg
     1261 agtggagata tttttgatga tgcagctaca ggtcaaggtt tgctgaattt ctttttccgt
     1321 gcactaaaca gcggagcaat tgatccggaa gatgtggaaa atgcaggagt gacggtggag
     1381 gaagtacagg ctggttcttt ccggaaaatt gtggaagcac gcaggtaggg gcaaattttg
     1441 tgtttgcccc cattcagttt tcagcgaatc aaattatgat tcattttgcg gcagcaccat
     1501 attcaaaatc aatgctgcaa gaccacctga agtgatggct gatccaaaaa tttgctgaat
     1561 cgcttttgga gtttggctga gaacctccgg tacaaaagca agcccaagtc cgattcccag
     1621 ggaaacggca ataatcagga attccctgcg ccctaaacgg cccatagaag caattatgtt
     1681 cacacccgct gcagccactg ctccaaacat aacaattgtt gctccgccta aaaccggatt
     1741 cggaagggag cggaagaacc cgccgatgac tggaaacaag cccagtataa cgagaaatcc
     1801 cgcgagatac atgcccacat aacgactggc aactccggtc atttggatga caccgttgtt
     1861 ttgactgaaa gtagtatttg gaaaagtatt gaaaatcgcg gc
//
LOCUS       DTVD01000154            1895 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_27449_length_1895_cov_324.714674, whole genome
            shotgun sequence.
ACCESSION   DTVD01000154 DTVD01000000
VERSION     DTVD01000154.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27449_length_1895_cov_324.714674"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="EYO68_05435"
     CDS             <1..222
                     /locus_tag="EYO68_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07283.1"
                     /translation="FDNAKRKIQIEQTVRGFLQLLDENELDLEDGLVAWNMLGFTIFQ
                     DTYPEENHDEIQQRMADFSKNLFDSGQLS"
     gene            222..1355
                     /locus_tag="EYO68_05440"
     CDS             222..1355
                     /locus_tag="EYO68_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07284.1"
                     /translation="MNSQHLVLIRVTIFRVFRRVSVLLYLWILFAPLLQAAEPAFIRE
                     SIRARGMGNAFTAAANDEMLLFYNPAALRSVYYNMYEVVGFNTTTNENTINLGKSSSS
                     FSTIGSLAGKKIYNEANLGLLSHVNSRFGWSFFSNGLVDIQVRNPIIPYLETKAYVQT
                     GLAGGMAWSFLDFQLDLGLGVKLVQRSGIDTKLHIFDEGIIEFTEDQKTTKLQKKFAS
                     KAAFAPDAGVIYHFDSYHNMEPKVAFSLQNIGGLDFEGAGKVPMTMNIGVSTESEFNG
                     FDMILAADYRDLADSQEMISKGNIMTERNIKIGVEFGWQKLFNGHHLISIRAGRNGPY
                     NSVGWSMNLFGFKVDFAKYSEEIGGYAGELEDKRTSLQVSLIF"
     gene            1510..>1895
                     /locus_tag="EYO68_05445"
     CDS             1510..>1895
                     /locus_tag="EYO68_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07285.1"
                     /translation="MEKLKSQYESSLQEQVSALREIRYISKHMGEPGKREIALRAMTF
                     FAFASDDGDIRDRSLSRLETVLESPEWPTYLKFAVIDSTVDLVTGELGFQEKHDGVLM
                     RFGVKSGIREDALKFLLSNFDQLTPEL"
BASE COUNT          556 a          324 c          439 g          576 t
ORIGIN      
        1 tttgataatg ctaaacgtaa aatccaaatc gaacaaaccg tccgaggatt tttgcagttg
       61 ctggatgaaa atgaacttga tttggaagat ggacttgttg cctggaacat gttaggattt
      121 actatctttc aagatacata tcctgaagaa aatcatgatg aaattcaaca acgcatggct
      181 gatttttcga aaaacctttt tgattcaggg cagctatcct gatgaacagt caacatttag
      241 ttttaattcg agtcactata tttcgagtgt ttagacgcgt atcagtttta ctttacttat
      301 ggatcttgtt cgctccattg ctccaagctg cagaacctgc ctttattcgt gaaagcatac
      361 gtgcccgtgg aatgggcaat gcgtttactg ctgcggctaa cgacgagatg ctgctgtttt
      421 acaatcctgc cgctttacgt tctgtatatt acaacatgta tgaagtagtt ggttttaata
      481 ctacaaccaa cgaaaacact ataaatctcg gaaaatccag tagcagcttt tctaccatcg
      541 ggtctttagc cggtaaaaaa atatataacg aggcaaatct tggtttattg agccatgtta
      601 attctcgatt cggttggtca tttttttcaa atgggctggt agatatccaa gtcagaaatc
      661 ctattatccc atatttagag acaaaagctt acgtgcagac gggacttgcc ggaggaatgg
      721 cgtggagctt tttggatttc cagcttgatt taggtctggg tgttaaacta gtgcaaagat
      781 caggaatcga tacgaagtta catatttttg acgagggcat cattgagttc acagaagatc
      841 agaaaaccac aaaacttcaa aaaaaattcg ctagcaaagc agcctttgcg cctgatgctg
      901 gagtaatcta tcactttgac agctatcaca acatggaacc aaaagttgcg ttcagcctgc
      961 aaaatatagg tggccttgat tttgaagggg ctgggaaagt tcccatgaca atgaacattg
     1021 gtgtaagtac tgagtcagag tttaatggtt ttgatatgat acttgcggca gattatcgtg
     1081 atttagcaga ctcgcaggaa atgatttcta aaggcaatat aatgactgag cgaaatataa
     1141 aaattggtgt ggaatttgga tggcaaaaat tatttaatgg acatcatctt atctcaattc
     1201 gtgcagggcg taatgggcca tacaacagtg ttggctggtc gatgaactta tttgggttta
     1261 aagttgattt tgcaaaatac tccgaggaga taggaggata tgcaggtgag ttggaggaca
     1321 aacgtacaag tttacaagtt tcacttattt tttaattaca atcttcaaaa accaagagat
     1381 ctcatgagaa atactacttt tgtgatattt gcactatcat tggtgtggtt atgtggttgt
     1441 attacgcagg gaagattgac ctcactgacc ttcaaaaaga gttttaatta taagactctg
     1501 gactcctcaa tggagaaact caagagtcaa tatgagagct cgctgcagga gcaagtcagt
     1561 gcattacgcg agatccgtta tatttcaaaa catatggggg agccgggtaa gcgggaaatt
     1621 gcattgcgtg ctatgacctt ttttgctttt gccagtgacg atggtgatat acgtgacagg
     1681 agtctctcgc gtcttgagac agttctggaa tcaccggaat ggccgactta tctgaagttt
     1741 gcagtgattg acagtactgt tgatctggtg acaggagaac ttgggtttca ggaaaagcat
     1801 gatggtgtgc tgatgcgttt tggtgttaag tcgggcatac gggaagatgc tttaaagttt
     1861 ttactgagta attttgatca gttgacacct gagct
//
LOCUS       DTVD01000155            1851 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_28499_length_1851_cov_164.532851, whole genome
            shotgun sequence.
ACCESSION   DTVD01000155 DTVD01000000
VERSION     DTVD01000155.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28499_length_1851_cov_164.532851"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(26..1768)
                     /locus_tag="EYO68_05450"
     CDS             complement(26..1768)
                     /locus_tag="EYO68_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIC07286.1"
                     /translation="MERKLSTIFASDVVGYSKMMGNNEEKTLETLGERREVIDSAIAE
                     HNGIIFGSAGDSVIAEFGSPVKATECAVQIQGKMKTMNEDIPVDQQMIFRIGINIGDV
                     MVSKDNLFGDAVNVAARLESAAQPSGICVSKTVFDLINQKIRVSFEDAGKLDLKNIVE
                     PIQAYFVIQKKGGVRFNQYEDSPQIKVEKAEQGSLAVMLFKNLSKDEDQAYFCEGFSE
                     DLITVLSKYSKLLVVSGNASFTYRDKSKSPKEIGKELGVRYILEGSVRKLGQKVRIGA
                     KLISADRENTIWSHNFDFTIDEMFDIQDELVETIVSTIVGHVDDEEVKQLVRVKPENH
                     TAYDLVLQGLEYHRKSNVTVENAKKAVEFFDQAIEVDPNCARAHAWRACSLSNYVGWR
                     PDEYGEDWIDQCADSVIRSLEIDQNDHEAHRIMGAISLVRGDFELARHHQERAMELCP
                     SDAYIMGKNAALLVYLGEPEKALEIVQHAMRINPFCPDDLFVDEGMCYFWLGKYSEAV
                     NCFRKMKTPDRESVFYLTASLSKLGEGIKSAETLKQAFKMTDLSVEDFLLTQHYQNPE
                     LKQELREILESIPI"
BASE COUNT          469 a          447 c          359 g          576 t
ORIGIN      
        1 gcttatgcgg ggcagtttat tttgttcaga ttggaatgga ttccagaatt tcccgaagtt
       61 cctgcttgag ctcaggattc tggtaatgct gtgtcagtaa aaaatcttcc acactcagat
      121 ccgtcatctt gaatgcctgc ttcaaagttt cagctgattt tattccttcc cccaatttag
      181 agagtgaagc tgtgaggtag aaaacacttt cacggtctgg tgttttcatt tttctaaaac
      241 aatttacggc ctcactgtat tttcccagcc agaaataaca catcccttcg tcgacaaaca
      301 agtcatcggg acaaaaagga ttgatgcgca tggcatgttg gactatttca agtgcttttt
      361 cgggctcacc gagatataca agcagagctg cgtttttgcc catgatgtat gcgtcgctgg
      421 gacaaagttc catggctctt tcctgatggt gtcgggcaag ttcgaagtca cctctaacca
      481 gtgatatagc tcccatgatc cggtgcgctt catgatcgtt ttggtcgatt tcgagtgaac
      541 gaatcacaga atcagcacat tgatcaatcc agtcttcgcc atactcgtct gggcgccagc
      601 caacgtaatt tgataaggaa caggcccgcc aggcatgggc ccgtgcgcag ttaggatcaa
      661 cttcaattgc ttgatcgaag aattcaacag cttttttagc attctccacc gtaacattgc
      721 ttttcctgtg atactcaagt ccctgaagta cgagatcata tgctgtatga ttctcaggct
      781 tgacacgcac caactgtttt acttcttcat catccacgtg tccaacgatg gtagagacga
      841 ttgtttcgac taactcatcc tgaatatcga acatttcatc aatagtgaaa tcgaaattat
      901 gagaccagat tgtattttca cgatcagcag atattagttt ggctccgatc ctgaccttct
      961 gacccagttt tcttacactt ccttcaagga tgtagcgcac tccaagttcc tttccaatct
     1021 ctttgggact tttcgatttg tccctgtagg taaagctggc attgcctgag acaaccaaaa
     1081 gtttgctata ttttgaaagc acggtaatga ggtcttcgga aaatccctca cagaaatatg
     1141 cctgatcctc gtctttacta agatttttaa aaagcattac tgccagtgaa ccttgttcgg
     1201 ctttttcaac cttgatttgg ggcgaatctt cgtattggtt aaaacgaaca cctccttttt
     1261 tctgaattac gaaataagcc tgtattggct ctacaatgtt cttcaagtcc agttttccag
     1321 catcttcaaa ggaaacccga attttctgat tgatcaaatc aaagacagtt tttgaaacgc
     1381 agattcctga gggctgtgct gcggattcaa ggcgggccgc cacatttacc gcatctccaa
     1441 aaagattgtc tttggagacc atcacgtccc cgatatttat tcctatcctg aaaatcattt
     1501 gctgatccac aggaatatct tcgttcatgg ttttcatttt accctgtatt tggacagcac
     1561 attcggtagc tttgaccgga ctcccgaatt cagcaatcac actatcgccc gcactgccga
     1621 aaatgatacc attatgctcg gcaatagcac tgtcgattac ctcccttctc tcacccaaag
     1681 tctccagggt cttttcctcg ttattcccca tcattttact ataaccgact acgtcggaag
     1741 caaaaatggt acttaatttt cgttccattt atttaatctt tttcaaatgg ggtaagccgt
     1801 tgtcttaagt gatttacctg agtgcccaat tcactgcatc ctccagccgg t
//
LOCUS       DTVD01000156            7519 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2851_length_7519_cov_280.734325, whole genome
            shotgun sequence.
ACCESSION   DTVD01000156 DTVD01000000
VERSION     DTVD01000156.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_7519_cov_280.734325"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            730..2694
                     /locus_tag="EYO68_05455"
     CDS             730..2694
                     /locus_tag="EYO68_05455"
                     /inference="COORDINATES: protein motif:HMM:PRK09631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIC07287.1"
                     /translation="MERKKMRYLKPLMDQNFLEYASYVIKDRAIPDIVDGLKPVQRRI
                     MHTMKEMDDGKFCKVANIVGDTMKLHPHGDASIGSALVVMANKEYFIEKQGNFGNLLT
                     GDPASAPRYIEARLTPLAKEALFNSELTEYIDSYDARNKEPVVLPSKLPVSILFGAEG
                     IAVGMSTRILPHNFNEVIKAQIAFLKNRPFKLLPDFFNGGLLDAGQYEEGNGKVRVRA
                     RIEITDEKILTVRELPFGVTTESLIQSVQDAVNKGKFKLSSINDFTAEKVEIELKATR
                     GMDAEKLLKLLFAFTQCEVSISVNMLLIQENHPAQLTVSEVLRYNTLRLKDLLKQELL
                     LSLEQARRKWHLRRMEMYFIGEKIYQKLEACDSYEEALEVVGKALLQIESVLHFPIVQ
                     DDIEKLLSLQIKRISRYDQKESQKELDKLKVTISSLNKSLRDITSYTITYLEKIKDKY
                     GSDYPRRTKIKIFDEIRVQDVAEKQKVCWDRKEGFLGMNVKSGTTSFYCSAYDKIVFI
                     RKDGSYQVIRVPEKQFIGKDVIFVEKLNAKTVHNVIYWEGASRVCYAKRFRVEKFILD
                     REYNLFTRKKGAKILHLSQGEGIILEIFFVPVPRIRKSKDIYILDDLAIKGIQARGNK
                     VSSKSVQSVKVTTVMPEQAQQPSLPEDTEN"
     gene            2775..2966
                     /gene="rpmB"
                     /locus_tag="EYO68_05460"
     CDS             2775..2966
                     /gene="rpmB"
                     /locus_tag="EYO68_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIC07288.1"
                     /translation="MSTKCQICGKTPANGNRVSHANNRTKRRWMPNLQTVRIRQANGN
                     RKKTRVCTQCIRTGKTIAA"
     gene            3042..3884
                     /locus_tag="EYO68_05465"
     CDS             3042..3884
                     /locus_tag="EYO68_05465"
                     /inference="COORDINATES: protein motif:HMM:PF00800.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07289.1"
                     /translation="MLLKDCTQIYTLGPQGTFSDEAAQKVLKTGVRVTYTGTFAEALA
                     KVTEDLGSAAVVPVENSVAGTVAQVQDSLVSNDLIILGEINLLIEYSLLANAELDQIE
                     VCFAHPQALEQSSGFITKNLGKAQNHFTRSNVDSGIRFLEAVDSGNKPVAAIVPATFA
                     QENSKWRKASAIQDYQNNTTRFLVVRSRKSDEQLDYSRKKTSLLVEFLDDRSGLLYQL
                     LSVFNLFKINLCRLESRPAKDTPWAYVFYVDFYNSADTEACLEVFSVSNFRYKVLGSY
                     DLIS"
     gene            3971..5188
                     /locus_tag="EYO68_05470"
     CDS             3971..5188
                     /locus_tag="EYO68_05470"
                     /inference="COORDINATES: protein motif:HMM:PF07238.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIC07290.1"
                     /translation="MFFLRNIFQKAGIFLGITSGWLLLCGIDRSPKQANFGLKDLQTK
                     NMLQSMPPLSEIPLSFYLLTSAFIASVVSAILLVRFLHKKKIREGLEKIAEDQAQLSV
                     DSEFEARQVDDEDREFIKIMCNSKDPVTLLPVIMSTKVFEKTAEDYKKSSKFSKSDLE
                     KIFLLRKSLQFTFKNTGVEFTCSQMLEPGTHLECVIHHKGKNVVFTTSILDSTEKQLL
                     IKPPRVNKRPANMKQFSELFCNIRRGKDADYEFKFKIIGQLTKDLNAVILSHTRNIKK
                     LQIRISERLPMDLEMDFLLVTAVNYEMMQGFDVGNLQQYALSGIIKDMSAGGIKVQIG
                     ELPTQGINKGDIFLFHLPNASLRGNLAATVLDVLSRRGRNEIHFAFRDTDILTHMKLN
                     QYLHRKKVNMEAA"
     gene            5211..>7519
                     /locus_tag="EYO68_05475"
     CDS             5211..>7519
                     /locus_tag="EYO68_05475"
                     /inference="COORDINATES: protein motif:HMM:PF01804.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIC07291.1"
                     /translation="MFRKIFYGISLLALLVIAGGYGFLRTKLPQRSGEIKLSGLKSKV
                     DVVFDKWGIPHIEAANEQDAYHALGYLNAQDRIFQLELMVRVAHGRLSEMLGEATLDV
                     DRYFRTLGIQRFAKKYAAEHFRNNPPKIQKALKAYLSGLNSFVASGPTPIEFTLLGIP
                     KREYSLVDLVSISAYMSYTFTNAVRQDPLVSYIHKKLGSNYLKDLAIYWPEGDTQIKV
                     SKKDAAEDLELAALFAEMDNLLDLIPPFFGSNSWVVSGSRTKSGKVILANDPHIGYAA
                     PATWYEAHLKTPDWELYGHHLAGIPFAILGHNHRMAWGVTMLQNDDLDYYREQANPEN
                     PDQVRFGNHWEDLKIIEETISVKGKVDVPLRIRISRHGPIINDVLESVGKTESQPVAL
                     AWQMLSNFENSMEQVFYELSHSESLADTQAAVSKLHAPGLNINYGDAEGNIAWWAAAR
                     LLIRPSHVNSFIILDGAGGRDEVLGYRDFSANPQAVNPRSGIVFNGNNQPGDVGNGLE
                     PGYYAPEERAHRMSQLLDPDKFDWTAADMRQIQLDTKHPRISNFRQHFMKVISESSVR
                     SEVFRQAAEVFKNWEGVHEKNALGPTIFHRLFYHLSISIFADEIEEKGTLVLLNSSLI
                     DKSMPLLLNNPQSPWWDNLQTPDKLENQQEILQTAWVNAVQSLEEDYGPDVNTWNWGR
                     IHTLEIQHLLGRKKPLNLIFNIGPFAVPGSKGVINQIRHKYGPKELKVSSGPSTRRVI
                     DFGDAENSTGISPTGQSGYFFDQHYQDQTP"
BASE COUNT         2254 a         1475 c         1719 g         2071 t
ORIGIN      
        1 gcggagaagt agtagactat cctgtgaata tgccgagtgt tcaggtttta agcagcggtc
       61 ctgtatcttc ttatacctcg cttgcagaaa aactgggtgt gttttcttcg caatatattg
      121 aatttactcc aactaattta gaaattattt accgcggcaa attagcaagg catgatggaa
      181 ctctcctgag actctgtttt ctgaagggtt tgctgcagag taagcaggat tatgtgagtt
      241 atgttaacgc ggatcagcag gctgaaaatg ttggtctgca tatcgaagaa aaagatgatc
      301 cgggctttac cgattatgaa agcgccctga aatgcacact tttcgcatcc ggcagtcagt
      361 ttactatagg cggtgttgtt tttagcgggc cccatccgcg aatcaacttg atcaatggtt
      421 ttgtctgtga atttgaggtg gaaggaacaa tattggcaac cattaatcag gatcgtcctg
      481 gaatggtggg ggtactggga acctgtttag gtaaaaatgg cgtcaatatc gaccagtttc
      541 aactgtcaag aaataaccgc ggcggcgagg cactgtcgct gattcgagtt gatgatgacc
      601 tgccggattc agtagttgaa gaaatccgaa agcaggatgg aatcacatta gtttgtaaaa
      661 tcgttttgta atgaattcca ggcaatttta atgcagacgt cctgtggtgt ctgtctggaa
      721 ctaatttgaa ttgaaagaaa gaaaatgcgt tatctaaaac ctttgatgga tcaaaatttt
      781 ctggaatatg cctcctatgt gattaaggat cgcgcaattc cagatattgt agatggtcta
      841 aaaccggtgc agcgtagaat tatgcacaca atgaaggaga tggatgatgg aaaattctgc
      901 aaggttgcaa atattgtagg cgatacgatg aagctgcatc cacacggaga cgcatccatt
      961 ggttcagcac tggtcgtgat ggctaacaaa gaatatttta ttgaaaaaca gggtaatttt
     1021 ggaaatttac tgactggtga tcctgcctct gctccaagat acattgaggc ccgccttact
     1081 ccactggcca aggaagctct ctttaattct gagttgactg aatatattga cagttacgat
     1141 gcaagaaata aggagcctgt tgtactgccc tctaaactac cggtatctat tctgtttggt
     1201 gcagaaggaa ttgcggtggg catgtctact cgaattttac cgcataattt caatgaagtt
     1261 atcaaagcac aaattgcttt tctgaagaac cggcccttca agctgttacc ggattttttt
     1321 aacgggggcc tgctggatgc cggacaatat gaagaaggaa atggtaaggt tcgggttcgt
     1381 gcccgcatcg aaattactga tgaaaaaatc ttaactgtac gggaattgcc gtttggagtg
     1441 acgactgaat ctttgataca gtccgttcag gatgcagtga acaagggaaa attcaaatta
     1501 tcctcaatca acgactttac tgcggaaaaa gttgaaattg aattgaaggc aacaagagga
     1561 atggatgcag aaaaactgct gaaactgctc tttgccttca ctcagtgcga agtctccatt
     1621 tcagtcaata tgctgcttat tcaagagaat catccggctc agctgactgt cagtgaagta
     1681 ctgcggtaca atactctaag gctgaaagat ttgctaaagc aggaattgct gctgtctctt
     1741 gagcaggcca gacggaaatg gcacctccgt agaatggaaa tgtatttcat tggtgagaaa
     1801 atatatcaga aactggaggc ttgtgattct tacgaagaag ctcttgaagt tgttggaaaa
     1861 gctctgcttc agattgaatc agtcctgcac tttccgattg tgcaggatga tatcgaaaaa
     1921 ttactgtcac tgcagatcaa acgtatttcc cgctacgatc agaaggaaag ccagaaggag
     1981 cttgataaac ttaaagttac aatcagttca ttgaataaat cattgaggga tattacaagc
     2041 tacacaatta cttacctgga aaaaatcaag gataaatatg gatcggatta tccgcgacgt
     2101 accaaaatca aaatatttga cgaaatccgt gttcaggatg tagcagaaaa acaaaaagtt
     2161 tgctgggacc gcaaagaagg ttttctggga atgaatgtaa agtccggtac tacttcgttt
     2221 tattgttctg cctacgacaa aatagtgttt atccgcaaag acggttccta tcaggttatc
     2281 cgtgttccgg aaaaacaatt tataggcaag gatgttatct tcgtggagaa actgaacgcc
     2341 aagacggttc acaatgtgat ttattgggag ggtgcaagtc gggtatgtta tgcaaaacga
     2401 ttccgggttg aaaagtttat tcttgaccgg gaatataatt tgtttaccag aaaaaagggt
     2461 gcaaaaattt tacacttgag ccagggagaa gggataatac ttgaaatttt cttcgtacct
     2521 gtacccagaa ttagaaaatc caaagacatt tatattcttg atgatttggc aattaaaggg
     2581 attcaggcca ggggcaacaa ggtttccagt aaatcggtac agagcgtgaa agtgactact
     2641 gtcatgccgg aacaagcaca acagccgtct ttaccggaag atacagaaaa ttaatggatt
     2701 gcatttaatt ataaaatgct ttagtatcaa agctctgaga tttagtttga acaatttgtt
     2761 tagaaaaaac cgtcatgtca acgaaatgcc agatttgcgg aaaaactccg gcaaatggaa
     2821 atcgggtaag tcacgcaaac aacaggacaa aacgccgctg gatgcctaat ttgcaaaccg
     2881 tgcgaatccg tcaggcaaat ggaaatcgta aaaaaactcg agtttgtact cagtgtatcc
     2941 gcaccggaaa aacgattgct gcttaagctg ccgaccttac aatctgccgg catacattca
     3001 aaacgccggc tgtttgcctg cctcttaact tcactgtttt catgctttta aaggattgta
     3061 cccaaatcta cactttggga ccgcagggta cttttagtga tgaagccgcc caaaaagtcc
     3121 tcaaaacggg agtccgtgtt acctataccg gtacctttgc tgaagcactt gcaaaggtga
     3181 cggaggattt gggttctgcg gcagtggttc cggttgaaaa ttctgttgcg ggtacagtgg
     3241 cacaggttca ggactctctg gtcagcaatg acctgattat tcttggtgaa atcaacttac
     3301 tgatcgaata ttctttgctg gccaatgcag aactggatca gattgaggtt tgttttgctc
     3361 atccgcaggc acttgaacaa agcagtggat ttatcacaaa aaacctgggt aaagcacaaa
     3421 accacttcac cagaagtaat gttgattctg gaattcggtt tctggaagca gttgattccg
     3481 gaaataaacc tgtagcggcg atcgttccgg ctacttttgc gcaagagaat tccaaatgga
     3541 gaaaagcttc agcaatccag gactatcaaa ataatactac ccgctttctg gtagtccgtt
     3601 cccgtaaatc agatgagcag cttgattatt cccgcaaaaa aacgtctctc cttgttgagt
     3661 ttctggatga tcgttctggc ttgctttatc aactgctgag tgttttcaat ctatttaaga
     3721 tcaacctgtg ccgtttggaa tccaggccgg caaaggacac accatgggcc tacgtctttt
     3781 atgttgattt ctacaattct gctgacacag aggcttgtct tgaggtgttc agcgtatcaa
     3841 attttcgtta caaagtactt ggaagttatg atttaatcag ctgattggca taccagatgc
     3901 gttactggaa cagaataata ttttttatga attttcatcc gcacatatat tgtcaaaacc
     3961 agagcaggtc atgttttttt tgagaaatat ctttcagaaa gcaggaatat ttttaggtat
     4021 cacttcaggc tggttgctcc tttgtggtat tgacagaagt ccaaaacagg caaattttgg
     4081 acttaaggat ctgcagacaa aaaatatgct gcagtctatg cctcccctct cagaaattcc
     4141 tttaagtttt tatctcctta cctcagcttt tattgcttca gtagttagtg ctattctact
     4201 ggtacgattt cttcacaaaa agaaaatcag ggaaggactg gaaaaaattg cggaagatca
     4261 ggctcaattg tcagtggatt ctgaatttga agccagacag gtggatgatg aggaccgtga
     4321 atttataaaa ataatgtgta actcaaagga tccagtaaca ttgttgccag tcataatgtc
     4381 tacaaaagta tttgaaaaga ctgcagaaga ctataaaaaa tccagtaaat tttcaaaatc
     4441 agatttggaa aaaatatttt tactgcgaaa aagtttgcag ttcacattta aaaacacggg
     4501 agttgaattt acctgctcac aaatgcttga acccggaaca catttggaat gtgtgattca
     4561 ccacaaggga aaaaatgtag ttttcacgac ttccattctt gactctactg agaagcagct
     4621 gctgataaaa ccaccgagag ttaataagcg ccctgccaat atgaaacaat tttctgaatt
     4681 attctgcaat atacgacgcg gaaaagatgc agattatgag ttcaagttta aaataatagg
     4741 gcagttgacg aaagatctca acgctgttat tctgagccac accagaaata tcaagaaatt
     4801 gcagattcgc atttccgaac gtctgccgat ggatttggag atggactttc tgctggttac
     4861 tgcagttaat tatgaaatga tgcagggttt tgatgtaggg aatctgcaac aatatgctct
     4921 ttccggaatt ataaaagaca tgagtgccgg aggaataaag gtccagatag gcgagttgcc
     4981 gacccagggg atcaacaagg gagacatttt tctttttcat ctaccgaatg caagtttacg
     5041 cggcaacctg gctgcaaccg ttcttgatgt gttatcaagg cgcggtcgta atgaaattca
     5101 ctttgctttc cgtgacactg atattctgac acatatgaaa ctcaatcaat atctgcatcg
     5161 gaaaaaagta aatatggagg ctgcctgatt ggaaaaattt tgctgacaaa atgtttcgaa
     5221 aaatatttta tggaatcagc cttttagctt tactggtaat tgcaggcggt tatggttttt
     5281 taagaacaaa attacctcag cgttcaggtg aaataaaact ttccggccta aaaagcaaag
     5341 ttgatgttgt ctttgacaag tggggaattc cgcatattga ggcagcaaac gagcaggatg
     5401 cctatcatgc tttaggttat ctgaatgcac aggatcggat ttttcagctg gaattgatgg
     5461 tccgtgttgc tcatggcagg ctttcagaaa tgctgggtga agcgaccctc gacgtagatc
     5521 gctatttccg tactctgggc atccagcgct ttgccaaaaa atatgctgca gaacatttca
     5581 ggaataatcc tccaaaaatt caaaaagccc ttaaagccta cctttccggt ctgaacagct
     5641 ttgttgcttc cggaccaact ccgattgaat tcacgctgtt aggtattcca aaacgtgaat
     5701 acagccttgt ggatctggtc agcatttctg catacatgtc ctacacattc accaatgcag
     5761 tccggcagga tccgctggtt tcttatattc ataaaaaact tggcagcaac tacctcaagg
     5821 acctggccat ctactggcca gaaggtgata cacagattaa agtttcaaaa aaagatgcag
     5881 cagaagatct ggagttggca gcactttttg cagaaatgga taatctgctg gatctgatac
     5941 ctccgttttt cggcagcaac tcctgggttg tttccggttc acgtaccaaa tccggtaaag
     6001 ttattttagc caatgatccc cacattggtt atgcagcacc ggcaacctgg tatgaagctc
     6061 acctgaaaac tcctgattgg gaactctacg gacatcactt ggcaggaatc ccctttgcga
     6121 ttctgggcca caaccaccgc atggcctggg gtgtcaccat gctgcagaat gatgatctgg
     6181 attattatcg tgagcaggcg aatcctgaaa atccggacca ggtccggttc ggtaatcact
     6241 gggaggatct gaaaattata gaagaaacaa tttccgtcaa gggaaaagta gatgttcctc
     6301 tgcggataag gatttcaaga catggtccca tcatcaatga tgtgctggaa tctgtcggaa
     6361 aaacagagag ccaaccagtt gcactggcct ggcagatgct gagcaatttt gaaaattcaa
     6421 tggagcaggt tttttatgaa ctgagtcact cagaaagcct tgcggataca caagctgcag
     6481 tcagcaaact ccatgcgccc ggtttgaata tcaactatgg agatgcagaa ggaaatatcg
     6541 cctggtgggc agcagcacgt ttactgattc gtccctcaca tgtgaattct tttattatcc
     6601 tggatggagc gggcggcagg gatgaagtcc tgggatacag ggatttctcc gcaaatcccc
     6661 aggcagttaa tccaaggtcc ggtatagtat tcaacggcaa taatcagccg ggggacgtgg
     6721 gaaatggtct tgaaccgggt tattatgcac ctgaggagag ggcacatcga atgagtcagc
     6781 tgcttgatcc agataaattt gattggacag cagcagacat gcggcagatt caactggata
     6841 cgaaacatcc acggatcagt aattttcgtc agcactttat gaaagtgatt tcagaatctt
     6901 ccgtacgcag cgaagtattc cgtcaggctg cagaagtatt taaaaactgg gagggagtgc
     6961 atgaaaaaaa cgctctagga ccgactattt ttcaccgact cttttatcat ttgagtatca
     7021 gtatttttgc agatgaaatt gaagaaaagg gaactctcgt tctactcaat tcttcactga
     7081 ttgataaaag catgccgttg ctgctgaaca atccgcaatc tccctggtgg gacaatctgc
     7141 aaacaccgga caagcttgaa aatcaacagg agatactgca gacggcctgg gttaatgcag
     7201 tgcagagtct ggaagaagat tatggtccag atgtgaacac ctggaattgg ggcaggattc
     7261 acacactgga aattcagcat ttgctgggac gtaaaaaacc gctgaattta atatttaata
     7321 tcggtccttt tgctgttccc ggaagcaaag gagtgattaa ccagattcga cataaatatg
     7381 gaccaaaaga actgaaagtc agttccggcc cctcaactcg ccgtgtgata gattttggag
     7441 atgctgaaaa ttcaactgga ataagtccaa ccggacagtc cggatatttt tttgatcagc
     7501 attaccagga tcaaacccc
//
LOCUS       DTVD01000157            7471 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2871_length_7471_cov_332.365696, whole genome
            shotgun sequence.
ACCESSION   DTVD01000157 DTVD01000000
VERSION     DTVD01000157.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2871_length_7471_cov_332.365696"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="EYO68_05480"
     CDS             complement(<1..650)
                     /locus_tag="EYO68_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopenicillin N synthase family oxygenase"
                     /protein_id="HIC07292.1"
                     /translation="MKPAEMPVTVKHPALPTIDLASAEKEIVAALDSAFSEVGFCICN
                     NAYLDPGIRESAFAASRAFHKLPESVKNSYAINSAHRGYISPKSSTSRTSSVAKVQRP
                     NTSESFMLMHEVLENDPRLGKPLQGPNQWPQEVPGFRDKMTAYWQDVNCMARKLAKLC
                     LKALGIEIGLYEAWFRQPTEFLRLLHYWPESMNEPEDSFGSAPHTDHGFLTLLVQDR"
     gene            complement(647..2095)
                     /locus_tag="EYO68_05485"
     CDS             complement(647..2095)
                     /locus_tag="EYO68_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC07293.1"
                     /translation="MIDLTKNEAKIQQNAKHCRERKIKLPTFGQMQNPETIPEEIKDE
                     LKNVGLWETHPANLFRISWKNEPVSEGGGFGGVNYIVIPPELSGVKAKIIALVGKWFP
                     TGAHKVGATYGCLAPALTTGQFCPGETKAVWPSTGNYCRGGAYVSSLLGCDSIAILPE
                     NMSRERFEWLEKVAGEVIATPGCESNVKEIFDKCWELKNTRNDIQIFNQFEEFGNPLW
                     HYMVTGKAAEEALKQEMGANSRFAGAISSSGSAGSMAFGYFLKEKFPGSKLAVAEALQ
                     CPTLLNNGFGDHRIEGIGDKHVPWIQDCKNTDMVIGVDDEAAVRLLRLFNEATGRETL
                     AHYGVDPEFAKQLDLLGISGIGNVLAAIKFAKYYELTEDDYVITILTDSMELYGSRIE
                     ELTAERGVYTIIDAHKDMQLMMDSGIENVLELTHYEKKRIHNLKYFTWIEQQGRELDE
                     LNSQWYDHDSYWNSIFSLAPQIDELIMEFNGK"
     gene            complement(2130..3323)
                     /locus_tag="EYO68_05490"
     CDS             complement(2130..3323)
                     /locus_tag="EYO68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIC07294.1"
                     /translation="MLSFNYKCSQCDAVYEIDPELMLCPVCSATQRSGQPLNGVLEVE
                     LKGSISSHDPLDFLPVEREFFPAIPVGNTPLWHPQNLVEKYAFPHLYLKDDGVNPTGS
                     LKDRASFLVAAFARKHEIREVVVASTGNAGSSMAGVGAAAGLKIKLFLPKTAPPAKMI
                     QALQYGADVVLAEKNYDQAFALSLEYTRTRGGMNRNTAYNPMTVEGKKTVSLEIFNQL
                     APDVPDGKAPDHVFVGVGDGVILSGIYKGFRDLLQLGRIAKIPTVHAVQASGSAAIYR
                     ALETGVFDGHPAKTVADSISVDVPSNGHLAVKNLKAYDGKCITVSDDEILSAQKELSS
                     ACGLFSEPAAATSYAGFLKQKSSLDSGETCVVLLTGNGLKDTNSAAKNIILPEKAINS
                     LDEIS"
     gene            complement(3571..7395)
                     /gene="dnaE"
                     /locus_tag="EYO68_05495"
     CDS             complement(3571..7395)
                     /gene="dnaE"
                     /locus_tag="EYO68_05495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIC07295.1"
                     /translation="MFTHLHLHTQYSLLEGAIRIKDLVTALKKNGFESCAVTDHGNMF
                     GAVEFFHTLKEEGLKPIIGMGASVIEDDPEEVLTESGPAKSSIGQTQFLCQNRLGYHN
                     LGFLASLSYTEGKVKGVPCINHQLLEKYNEGLIALSGGMKGQINKHFLDGRPDDARRV
                     ALWYRDVFSNRYYIELQNTGLPEQQELNQQLIRLGHELDIPLVGTNDCLYLTPEEAEA
                     QYILWLMGLQRRVTDEGVPQQLGSQRYLKSPEEMLSAFAELPPTALNNTSKIAEQCDV
                     SLENKKTFLPQIATRESETLDSKLREDAEKGLKNRLVKLYELYAPQVPFEEFREPYSE
                     RLSFELEVIIQMEFPGYFLIVSEFIKWAKDNGVSVGPGRGSGAGSLVAYALLITDIDP
                     LRYGLLFERFLNPYRVNLPDFDIDFDVEGREKVIEHVREKYGDKNVCQISTFGSLKAK
                     AVVRGVARVLDFPYSEADKIAKLVPNDLNITLDQALAKEPELALLAAEGSENEQRLIK
                     FSRQLEDLNTHMGTHAAGVIIMDQDIREVMPVCTGKEGALQSMYPMKYAEDQGAVKFD
                     FLGLQNLSTIDNTLDLIKTSRPDSASIDISSIPMDDSLTFDLFCRADTIGVFQLESSG
                     MKRLVSNMQPSVFGDIVAILALYRPGPLGSGMVEDYVQCKHGRKRVIYPHPLMADILR
                     ETYGVLVYQEQIIQGVQVLAGFSLGQADLLRRAIGKKTPEVLAEQRLQFVEGCLKNPE
                     FVEKCPKSSTPEEKANEIFDTINYFSGYGFNKSHSVAYGLISYQTAFLKAHYPVQLMA
                     ALFNGSINNQDNVISYISECKAMGVNVLPPDVNHSAKTFTVAPTGFRISVRTISHFER
                     DFCGTSPAKETVPEEWIEPLRNSMKILKNRDFKEEAEFLEFFAQTTESSSGKLNTLNS
                     KVNIAIFDLKSPFSAWLRREARIEVIRFGLNAVKNVGSKAVDAILKVRNEHGQLTDFM
                     EFMKKVDLKEVNKRMIETLVKCGAFDSLHENRSQLFAAIDSAFHLAQEFHRAEEPSQD
                     SLFELMDAGDAEATKTQLEFPEVRNWPKRERLNQEKTALGFYVSGHPLDSYVSEIKIL
                     ATTTAKMKEGLHAEKDKVSLVGIVVNNTVRLNQSNEKFAIVTLEDTRGTIEFPVFASV
                     YEKVGELLESDDPLLISGRVNYRDEEVGMFVDNVHRLSELRETEAKSMLIKIGAESLN
                     QESVSLLRKTLQKYSGDKPFTFSVQTPEAVSVTITPEERINITSTLIEELEEILPHQT
                     LEFGYPSLKNFR"
BASE COUNT         1977 a         1784 c         1586 g         2124 t
ORIGIN      
        1 cgatcctgca caagcagcgt gagaaaccca tggtccgtgt ggggtgcgga tccaaaagaa
       61 tcctccggtt cattcataga ttccggccaa tagtgcagca aacgcaaaaa ctcagtaggt
      121 tgccgaaacc acgcttcgta caatccgatc tcgattccga gtgcttttag acacaacttt
      181 gccaacttgc gggccatgca attcacgtcc tgccaatacg cggtcatctt gtcccgaaac
      241 cccggaactt cctgcggcca ctggttcggt ccctgaagcg gtttacctaa acgtggatcg
      301 ttttcgagca cctcgtgcat cagcatgaac gattcagagg tgttgggtct ctggaccttt
      361 gcgactgagg aggtacgtga ggtgctggac ttgggggaga tatagcctcg atgggccgag
      421 ttgatcgcgt agctgttttt cactgattcc ggaagtttat gaaacgcacg ggatgcggca
      481 aaggcggatt cccggattcc cggatcgagg tacgcattgt tgcagatgca aaatccaacc
      541 tcgctaaaag cggaatccaa tgcggctacg atttcttttt cagcgctggc gaggtcaatt
      601 gtggggagtg cgggatgttt cacagtaaca ggcatttcag cgggtttcac ttcccgttaa
      661 attccataat caactcatca atttgcgggg cgagcgaaaa aatgctgttc cagtaagaat
      721 cgtgatcgta ccattgtgaa ttcaattcat ccagttcacg tccctgctgt tcaatccagg
      781 taaagtattt gagattgtgg attcttttct tttcataatg tgtcaattcc agcacatttt
      841 caattccgga atccatcatc aactgcatat ctttgtgtgc atcaatgatt gtgtatacgc
      901 cccgttctgc tgtcagttct tcaatacgcg aaccgtaaag ttccattgaa tctgtcaaga
      961 ttgtaatcac gtaatcgtct tcggtcaact cataatattt tgcaaatttg attgctgcaa
     1021 gcacgtttcc aatacctgaa attcccaaca ggtccaactg cttcgcaaat tccggatcca
     1081 caccgtagtg cgcaagtgtt tcccgcccgg tggcctcatt gaaaagacgc agcagacgaa
     1141 ccgcagcctc atcatcaact ccaatcacca tatccgtgtt tttgcagtct tgaatccacg
     1201 gtacatgttt gtcgccaata ccttcgatac ggtgatctcc aaaaccgttg ttcaacagag
     1261 tgggacactg cagggcctcc gcaacagcca attttgaacc gggaaatttt tctttcagaa
     1321 aatatccaaa ggccattgaa cctgcagatc cggaagagga aatggcaccg gcaaatctac
     1381 tgtttgcacc catttcctgt tttaaagctt cttcagcagc tttgcctgtt accatataat
     1441 gccaaagcgg atttccaaat tcttcgaact gattgaagat ctggatatcg ttccgcgtgt
     1501 ttttcagttc ccagcatttg tcaaatattt ccttcacatt gctttcacaa cccggagttg
     1561 caatgacttc accggccact ttctccaacc attcaaaacg ttcacgcgac atattttccg
     1621 gtaaaatcgc aattgaatca cagcccagca gcgaagacac ataagccccg cctctgcagt
     1681 agtttccggt ggatggccag actgccttag tttctcccgg acaaaattgt ccggtggtta
     1741 aagccggagc caggcagccg tatgtcgccc ccactttatg cgcaccggtt ggaaaccact
     1801 tgccgaccag tgctattatt tttgctttga caccggaaag ttccggagga ataacgatgt
     1861 aattcacacc gccgaaacca ccgccctctg agacaggttc atttttccag gagatccgaa
     1921 acaaattggc cggatgtgtt tcccacaaac caacgttttt cagttcatct ttgatttctt
     1981 ccggaatcgt ttccggattc tgcatctgtc caaaggtggg aagtttgatt tttcgttccc
     2041 ggcagtgttt tgcgttttgc tgaatttttg cttcgttttt tgtcaaatca atcatattta
     2101 tttagattag aaatttagta gtttcagttt tatgaaattt catcaagcga gttgatggcc
     2161 ttttcaggca ggataatatt ttttgctgca gaattagtgt ctttcagtcc gtttccggtc
     2221 agcagaacaa cacaagtttc accggaatcc aaagatgatt tctgtttcaa aaaccctgcg
     2281 taagacgtag ccgccgccgg ttcagaaaaa agtccgcagg cggaagaaag ctctttttgt
     2341 gcagacaaaa tttcatcatc cgaaacagta atacatttgc cgtcgtaggc ttttagattt
     2401 ttcacagcaa gatgtccgtt gctcggaaca tcgactgaaa ttgaatccgc aacggttttt
     2461 gccggatgtc cgtcaaaaac tccggtttcc agtgcacggt aaatcgcagc gcttccggaa
     2521 gcctgtactg catgaacggt gggaattttg gcaatcctgc caagctgcag cagatcccgg
     2581 aatcctttgt aaatacccga caaaattact ccgtctccaa ctccaacaaa aacatgatcc
     2641 ggcgcttttc catccggaac gtccggtgcg agttgattga atatttctag ggagacagtt
     2701 ttcttcccct caacagtcat tggattgtaa gcagtgttgc ggttcattcc accacgggtt
     2761 ctggtatatt ccaatgaaag tgcaaaggcc tgatcgtagt ttttttccgc caaaaccaca
     2821 tctgccccat attgcagggc ctggatcatc tttgcaggag gtgcagtttt tggtaaaaat
     2881 aatttgattt tcaatcctgc tgccgcaccg actcctgcca tggaagagcc cgcgtttccg
     2941 gtagaggcca caacaacttc acggatctcg tgttttcggg caaaagctgc caccaaaaaa
     3001 gaagccctgt cttttaatga accagtggga tttactccat catccttcaa atacaagtgc
     3061 ggaaaagcat atttttccac aagattttgc ggatgccaca aaggagtatt tccaacagga
     3121 attgcaggaa aaaactcgcg ctccaccggc aaaaaatcca atggatcgtg actggatata
     3181 ctccctttaa gttccacttc cagcacgccg ttcagaggtt gtccggatcg ttgcgtggca
     3241 gagcagacgg gacacaacat cagctccgga tcaatttcat aaactgcatc gcactgactg
     3301 catttgtagt tgaagcttaa caatagtaac tctttctatt ctaaagttgc tgtcgcataa
     3361 ttccggatta agcaacaatt tgtcttttgt catcccggac ctgttccggg atccagttca
     3421 ctgaacgggt tgaatacaag aattgaagtg attcaatgga ttcctggtct ccgctgcact
     3481 gcccggaatg acaattctaa attccggtat tttcactgcc acaagcttac tggaatgtgt
     3541 gacaaaaaac gagtaataat tactctataa ttagcggaag tttttaagag atgggtaacc
     3601 aaattccagc gtctggtgag ggagaatttc ctccagttct tcaatcagcg ttgaagtaat
     3661 gttgatccgc tcttcggggg taatggttac agaaacggct tcaggtgttt gaactgaaaa
     3721 tgtgaaaggc ttgtcaccag aatatttttg cagagtcttc cgcagtaaac tgacagattc
     3781 ctgattcagg gattcagcac ctatttttat caacatgctt tttgcctcag tttccctgag
     3841 ttcactcagc ctgtgtacat tgtccacaaa cattcccacc tcttcatccc gataattcac
     3901 acgaccggaa atcaaaagtg gatcgtcgct ttccagcaat tctcccacct tctcataaac
     3961 cgaggcaaag acaggaaact caatggttcc ccgtgtatct tcaagtgtga caatggcaaa
     4021 cttttcgttg ctttggttta gacggacagt attattgaca acgattccaa ccaaagaaac
     4081 cttatctttt tctgcatgca gaccctcttt cattttagca gtggtggtag ccaggatttt
     4141 aatttctgag acataactgt ccagcggatg tcctgaaaca taaaagccca gtgccgtctt
     4201 ttcctgattt aaacgttccc gtttgggcca gttcctgact tcaggaaatt caagctgagt
     4261 tttggtagct tctgcatcgc ctgcatccat caattcaaac agggaatcct gcgaaggctc
     4321 ttcagcacga tgaaattcct gcgctaaatg aaaggcactg tcgattgcag caaacaattg
     4381 cgaacggttt tcatgcagtg aatcgaaggc accgcacttg accagtgttt caatcattcg
     4441 tttattgact tccttcaaat ccactttttt catgaattcc ataaaatcag tcaattgtcc
     4501 atgttcgtta cgtaccttca aaattgcatc aactgctttt gagcccacgt ttttaacagc
     4561 attcaaacca aaacgaatga cctcaatccg ggcttcccgg cgcagccaag cagaaaatgg
     4621 ggacttcaaa tcaaagattg caatattgac tttagagttt agtgtattta atttcccgct
     4681 tgaagattca gtggtttgag caaaaaattc cagaaattca gcttcttctt tgaaatcgcg
     4741 atttttaagt attttcatgg aattccggag cggctcaatc cattcttcag gaactgtttc
     4801 ttttgccgga ctggtaccgc agaagtcacg ttcaaagtgc gagattgttc tgactgagat
     4861 tctgaaaccg gttggtgcaa ccgtaaaagt ttttgcgctg tgattcacat ccggaggcaa
     4921 aacattgaca cccattgcct tgcattcact aatgtaactg atgacgttgt cctggttgtt
     4981 gattgaacca ttgaacaatg cagccatcag ttgaacggga taatgtgcct tgagaaatgc
     5041 agtttgatat gaaatcaaac cataggcaac actgtgggat ttgttaaaac cgtaacctga
     5101 aaaataattg attgtgtcaa atatttcatt ggctttttct tccggagtac tggatttagg
     5161 acatttctca acaaactccg ggtttttcag gcagccttca acgaattgta gacgctgttc
     5221 agccagaact tccggagtct tttttccaat cgcacgacgt aacaaatctg cctgtcccag
     5281 agaaaatccg gcaaggacct gcacgccctg aatgatttgt tcctgataga caagcacacc
     5341 gtatgtttcc cgtaaaatat ccgccataag tggatgcgga taaataactc tctttcgtcc
     5401 atgtttgcac tgcacataat cctcaaccat tccggaacca agagggcccg ggcggtaaag
     5461 tgccagaatc gcaacaatat ctccaaaaac tgagggctgc atgttagata ccaggcgttt
     5521 catacctgac gattccagct ggaaaactcc gattgtatct gcacggcaga ataaatcgaa
     5581 agttaaggaa tcgtccatgg gtatactgga gatgtcgatt gaggctgaat ccggacggct
     5641 tgttttgatc aggtcaagag tattgtcaat ggtggaaagg ttttgcaaac cgaggaaatc
     5701 aaacttgacc gcaccctggt cctctgcata tttcattgga tacatcgact gcagcgcacc
     5761 ttcttttccg gtacagaccg gcatcacttc acggatatcc tgatccatga taatcactcc
     5821 agcagcgtgg gttcccatat gagtgttcag gtcttccagc tgccggctga acttaatcaa
     5881 acgctgttca ttttcactgc cttctgcagc aagcagcgcc agttcaggct ctttagccag
     5941 cgcctgatca agcgtgatat tcaaatcatt tggaacaagt tttgcgattt tatctgcttc
     6001 agaatatgga aaatccagca cccgcgctac accgcgcacg accgcttttg ctttgagtga
     6061 accaaaagtg gatatttggc atacattttt gtcgccgtat ttttcacgaa cgtgctctat
     6121 tactttttct ctgccctcta catcaaaatc aatatcgaaa tccggcaggt ttacacgata
     6181 tggattaagg aatctctcaa aaagcagccc gtagcgcagg ggatcaatat ccgtgatgag
     6241 taaagcgtaa gcgaccagcg aacctgctcc ggaacccctt ccagggccaa ccgaaacgcc
     6301 gttgtctttt gcccatttga taaattcaga aacaatcaaa aagtaacctg gaaattccat
     6361 ttgaatgatc acctccagtt caaaacttaa acgctcagag taaggttccc gaaattcctc
     6421 aaacgggact tgcggagcat aaagttcata aagttttacc aaacggtttt tcaatccttt
     6481 ttcggcatct tcgcgcagtt ttgaatcaag agtttcagat tctcttgtgg caatttgcgg
     6541 cagaaatgtt tttttatttt ccagagagac atcgcattgc tctgcaattt tactggtatt
     6601 attcaatgca gtcggtggaa gttccgcaaa tgccgagagc atttcttccg gacttttcag
     6661 gtaacgctga cttccaagtt gttgaggaac accttcatct gtcactctgc gctgcaggcc
     6721 catcagccag agaatatact gtgcctcagc ttcttccgga gtgagataaa ggcagtcgtt
     6781 tgtgccgacg agaggaatat cgagttcatg tcctaaacgt attaactgtt ggttcaattc
     6841 ctgttgttcc ggaagtcctg tattctgtag ttcaatataa taacggttgc taaaaacatc
     6901 tctgtaccaa agcgcaactc gacgtgcgtc atccggacgt ccgtcaagaa aatgtttgtt
     6961 gatctgtccc ttcattccgc cgctgagggc aatcaaaccc tcgttatatt tttccagcag
     7021 ttgatgattg atgcagggga ctcccttgac tttaccttcg gtgtagctga ggctcgccag
     7081 aaaacccaga ttgtgataac ccagccgatt ctgacaaaga aattgagtct gtccaatgga
     7141 agattttgca ggtccggatt cggttaaaac ctcctcaggg tcatcttcaa taacagatgc
     7201 acccattccg ataatcggct ttaagccttc ttccttcaaa gtgtggaaaa attcaacggc
     7261 accaaacatg tttccatgat cggtaacagc acaggactca aaaccatttt ttttgagtgc
     7321 agtaaccaaa tctttaattc ggattgctcc ctcaagcaga gaatattgcg tgtgaagatg
     7381 aaggtgggtg aacattactg tctgaattgt tttttggaag agtgaccagt atgcatctta
     7441 catgaaatta attttgcggg caagattctt a
//
LOCUS       DTVD01000158            7456 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2883_length_7456_cov_274.317525, whole genome
            shotgun sequence.
ACCESSION   DTVD01000158 DTVD01000000
VERSION     DTVD01000158.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2883_length_7456_cov_274.317525"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(430..2163)
                     /locus_tag="EYO68_05500"
     CDS             complement(430..2163)
                     /locus_tag="EYO68_05500"
                     /inference="COORDINATES: protein motif:HMM:PF12895.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07296.1"
                     /translation="MTLTTCFKYTIIFCTARLPSFLIAKEIKNQFLRKLLPLLFTSLL
                     FVNLVSPQFLQARQSVGLLSVDVKYPSKGLNWLGLFVQEELSMQLQLADRFSVITPDI
                     MRRWNQRLRDSGQLTSSNISLQNSEISQIKPNRLIQISLQKVLNQLSVTLSISIFGKS
                     KSTLKIQNTHTWNSPDKLIVSLLQSLGEGDKFFSNLTHFPKTYDWDGLKHFYQWRLKQ
                     VPVPNTSAWEAHKDELEALLISYPSLASSIQFYRAVMLIIESRVLEPALVPALNSAET
                     EILAAMKQHPDNSEHHTLLSLVHYLRREPLFAKQQANIANQLNPRDGMSLILYGLTIG
                     QTPQAGDAFVKRGLKLYPFVTEPSTDGWQPYHVLIKDLEPWMIPALSEKTQSFEQLMH
                     SGQQDYNARRWKEALQSFEDAAALQPLQPEPHLYLAKLRLVQQDPQSALLLLAKLRKK
                     FPKHPEINLYLGYAHEKLKHLEKAETLYRQVLHLKPEHHKALLRLGAVLIKLGKRHEA
                     RSFLESLTRKYPIYSVAWWNLGIVYYQLGELQLAETAWEESLRLEPDNSQIRLRIEQL
                     REESSYIPATL"
     gene            complement(2415..2591)
                     /gene="zapB"
                     /locus_tag="EYO68_05505"
                     /pseudo
     CDS             complement(2415..2591)
                     /gene="zapB"
                     /locus_tag="EYO68_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002535264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
     gene            complement(2946..4889)
                     /gene="dxs"
                     /locus_tag="EYO68_05510"
     CDS             complement(2946..4889)
                     /gene="dxs"
                     /locus_tag="EYO68_05510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIC07297.1"
                     /translation="MKSLLESISQPTEIQNLNLQELTQLAEECRQRIIKVTSRRGGHL
                     ASSLGTVEITVALLKNFNFNIDRIVWDVGHQAYAYKILTGRNEKFDSLGKAGGIKKFL
                     SRDESSFDHFGAGHASTSISAALGMAIGRDLQQKKHRVIAVIGDGAMTGGLAFEALNH
                     NGALDKNMLVIYNDNSVSIDPNVGAISKLLTRFASSRFYNSFREETLEFAEKAPFSER
                     LGLKTTLQKLHDSAKSFFSPPSMLFEQLGWRYFGTVDGHDLPELLDLINHVKDLDGPI
                     VIHAITQKGKGYAFAEEDAHKYHGVTPFEPEDGKFVKKKSSGNAISFSQVFGNKLGEL
                     MARDEQVVVVTAAMLSGTGIVKLQPKYPERVLDVGIAEGHAVTCSAGLATTGSKPFVA
                     IYSTFLQRALDHIIHDVAIQKLPVRFMLDRAGFVGADGPTHHGLYDLTYLRMIPNMTI
                     MVPRDGEELRGMMELAYNNESGPSAIRYPRGNTANPDENDCSQLEFGKAQILRKGKDI
                     ALFAIGLMVDIAEQTADLLEKNGYSVAVVNARFVKPLDEDVIVRLGREVQLLVSLEEN
                     TIHGGFGSGVLETLSVSGICVPTLQLGVPDRFIAQGNPDEQLRSAELSMEQIYSRILE
                     RLPVPAHKKIRKKRTVSPKILAS"
     gene            5043..7442
                     /locus_tag="EYO68_05515"
     CDS             5043..7442
                     /locus_tag="EYO68_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07298.1"
                     /translation="MSTNTLEQLKQAKSALEAERKPVSHVQEAVKATKDITQFVKIAL
                     SKENRWIFQAGEQDSIVSILAEINRTNKELYNKCRTPEHFNRDATRYLKMKNHIIRKS
                     ECIHFSLDQGFYATEPLGFSAGKAIELSLKQIRKIRDGHQQGKWTRLPIDVNVDDSAK
                     TEVEKIYAESAPLAEKLLKNENIGNFDPPMTIEEVQYVYKFFHNVHQIQELEQQALTD
                     ALSKKTPAEQSTPNEILDQLSSFEKKLKNVRVPTSKNIPDWGIHLYEMSLTDMTLRMH
                     LLRIPFEPLGFLNRHLKILEEQKDSDGIEKSISSFHDNAVQELEKLQLFSESFAGVDE
                     TLQEVTGILQNLSICFGKIYHNASSQTITNSFLIKVLELIKGMLEKGGRVADSKSDTL
                     LKLHQSVNDVDLFDDPITEGMIHSLDLLEQGKEAMSQYCDKLKTFLETLSNASDWDSR
                     QTYKFLKNAYDENASLTSASMFFGSITKTCVLLKSSIKEVESMWLELAKLREQKLDSG
                     ALYEQTIALLGQDTGFSENSLRQRADQVEELNWILDEMLGLGVQEEQRFVDELHSELI
                     PVSDRIKALRIKESARKVELTSTKEKSEQDSIVVKSKVRKTKKSDKSSELAIGNKKPT
                     YDPFQPFQMVALKKACKEMTSSEGKMIYQVLTNTHIFFTLLNKMESSFKQGEGLENEL
                     EQDLARSKTTQEFLMNFARLKFLPNGYYSENNTPFDYNEEKRQFEVPEGIKKQLKITD
                     SSTSSPMDIFSRLIRVLLGLDDSNTLAAIMPPQNLSIMEDDFMLKRKDRKAVEEGLQS
                     T"
BASE COUNT         2277 a         1478 c         1491 g         2210 t
ORIGIN      
        1 cctgtcttgg attttttaaa gtacgaactg gcgtatatat actctcctga caaatttgtc
       61 tttgaagttt catctgtcgg tgcgacctcc tgactatcag tttgagctaa aacctggaca
      121 gaaaaaaaga tagatatgag taatattatg agttttttca tgttcaattc cttcttctgc
      181 tcttaattaa tttgaaaatc tttttccaac ctagcatgaa agtttattgt ttactaaaga
      241 gtattgcaag ctttttattc caaaaaaacc gtatgatata ctttttttaa gttaaaatac
      301 actataaaaa agtatttttc cacaaaaaaa ttatttcagc ttcaaataca cctaatattt
      361 tatgcaaaac aaactgtata ttacaacaat tgcaagtgtg aagcctaatt gattatttgt
      421 ttaataattc tacagtgtgg ctggtatata tgaggattct tcacgcagtt gttcgatgcg
      481 taacctgatt tgactgttat ccggctcaag tctcaacgac tcctcccacg cagtttctgc
      541 taactgcaat tctccaagct gataatagac aattccaagg ttccaccagg ccacagaata
      601 tataggatat tttctagtga gactttccag gaaactgcgg gcttcatggc gttttccaag
      661 ttttataagc acagctccaa gcctcaacag tgctttatga tgttcaggtt tcaggtgaag
      721 tacttgtcgg tataatgttt cggctttttc cagatgtttt agtttttcat gtgcataacc
      781 cagataaagg ttgatttcag gatgtttggg gaatttcttt cgaagtttag caagtagtaa
      841 aagcgcactt tgcggatcct gttgaaccag gcgtaatttt gccagataca gatgaggttc
      901 tggttgtaat ggttgcagtg cagcagcatc ttcaaaagac tgaagtgctt ctttccagcg
      961 gcgggcattg taatcctgct gaccggaatg catcagctgt tcgaaacttt gtgttttttc
     1021 ggaaagtgca ggaatcatcc atggttctaa atccttgatc agcacatggt aaggctgcca
     1081 gccatcagta cttggttcgg taacaaacgg ataaagtttt agcccccgct tgacgaaagc
     1141 atcacctgcc tgaggggttt gtccgatagt aagtccatag aggatcaaag acataccatc
     1201 gcggggattt aactgattgg caatatttgc ctgttgttta gcaaacaagg gttctcttcg
     1261 aaggtagtgc acgagcgaga gtaaagtgtg atgctcgcta ttatcagggt gctgtttcat
     1321 ggctgccaaa atttctgttt ctgcagaatt tagagcaggg acaagagctg gttctaaaac
     1381 tctgctttcg ataatgagca tgacagctct gtagaattga atcgatgatg ccagagaagg
     1441 ataactaatt agcaatgcct ccaactcatc tttatgtgct tcccatgcag aagtattagg
     1501 gacaggtact tgtttaagtc tccactgata aaaatgcttt agaccatccc aatcgtaggt
     1561 tttggggaaa tgagtcagat tactgaaaaa tttgtccccc tcaccaaggc tttgcaagag
     1621 tgaaactatt agtttatcag gagagttcca ggtatgagtg ttttgtattt tcagagttga
     1681 tttacttttt ccgaaaatac tgatgctcaa tgtaaccgaa agttggttaa ggactttctg
     1741 taaagatatt tgaattagtc tgttcggttt tatttgggaa atttccgagt tttgtaatga
     1801 tatattactt gaggtaagct gtccggagtc acgaaggcgc tgattccagc ggcgcatgat
     1861 atccggagtt attacagaga atcgatcggc cagttgcagc tgcatggaaa gttcttcctg
     1921 aacaaacagc cccaaccaat tcaatccttt tgaagggtat ttgacatcaa ctgaaagtaa
     1981 tccaactgat tgacgtgcct ggagaaactg aggagacacc aggtttacaa agagcaggct
     2041 tgtaaacagc aaggggagaa gtttgcgaag aaactgattt tttatttcct tcgcaattaa
     2101 aaatgaaggc aaacgagcag tgcaaaaaat tatggtatat ttgaaacacg tcgttagcgt
     2161 catgcgtagt gttgtgagga tcccggggct tcagtgtatg aatctgcact ggtaccaaaa
     2221 gtggaccctt caattttctc tccttcaaat tcttccgtat ccccctcagc ctcctcatcc
     2281 tcagtctcca cagcaaccac ttcctctgcc tcaacttcct ccgcctcagc ctcctcagca
     2341 accacttcct cagcctcttc gtctttttga attgacgtat caacctctgc ctcaacttca
     2401 acagcttcca catcttccag ctgttcaaat ttttcaatta aggatgatag cctttgctcc
     2461 caatcttcat atcgattttc caactcatca ttcttttctt ttaaatccgt aatttccatt
     2521 cgtgaaaatt cgatggtttc aatcgcttca ttaatttttg cttcaagctg tttcagtaaa
     2581 tcgtttgaca tgtttaattt ccttaaaaaa tggttgtagc aacttcctca cggaattcgt
     2641 taaccggatc cccggcaaaa ctttcagcac aactctgatg caagagctgt acattttagc
     2701 ttatacttga atgaaaaagg taatcaacaa taataacaat tgattgcttg taattcctga
     2761 aataaaggtt taacaaaaaa agtatgcaac tgtcagtcac ctactttcgg cctgattttt
     2821 cagactaatc gttgattgat gaaaactggg gaccatacaa ttgggcaata acaaatcagc
     2881 tgaaacggag atagaagccg gtttagataa tattgttcag gcactgttta ataattcttc
     2941 aattattatg aggcaagtat ttttggtgag actgttcttt tctttctgat tttcttatga
     3001 gcaggaacgg gtaatctttc aagaatacga gagtagattt gttccatact caattcagcc
     3061 gaccttagtt gttcatccgg gttcccttgt gctatgaagc gatcaggaac tccaagctgc
     3121 agtgtaggca cacaaatacc acttacactt agagtttcaa gcacaccact gccgaaacct
     3181 ccgtggattg tgttttcttc aaggcttacc agtaattgta cttctctacc aagcctaaca
     3241 attacatcct catccagcgg tttgacaaac cttgcgttta caacagcaac tgagtaacca
     3301 ttcttttcaa gaagatcagc tgtctgctct gcaatatcga ccattaaacc tatcgcaaac
     3361 agcgcaatat ctttcccttt tcgtaaaatt tgtgctttgc caaattcaag ttgagaacaa
     3421 tcattttcgt caggattagc agtatttcct ctagggtagc gtattgccga gggaccgctt
     3481 tcattattat aggcaagttc catcatgccg cgcaattcct ccccgtcacg gggtaccatt
     3541 attgtcatgt tcggaatcat acgcaggtaa gtcaagtcat acaatccgtg atgggtcggg
     3601 ccatcagctc caacaaatcc tgctctgtcg agcatgaagc gtaccggtaa tttttgaatt
     3661 gctacgtcgt gtataatgtg atcaagtgca cgctgcaaaa atgtggaata aatggcaaca
     3721 aatggtttac taccggtggt tgcaagtcct gctgagcacg tcacagcatg accttcagcg
     3781 ataccgacat caagcacacg ttccggatat tttggttgca gtttaacgat acccgttcca
     3841 ctcagcatgg cggcagtcac aaccacgact tgttcatctc gtgccatcaa ttctcctagt
     3901 ttgtttccaa aaacctgaga aaaactaatt gcatttccgg atgatttttt ctttacaaat
     3961 tttccatcct ctggttcgaa tggggtaaca ccatgatact tatgggcatc ttcttcagca
     4021 aaagcatagc ctttaccttt ttgtgtaatg gcgtggatga caatgggacc gtctaaatcc
     4081 ttaacatgat ttattaaatc cagcagttct ggaagatcat gaccatcgac tgtaccaaaa
     4141 tatcgccaac ccaattgttc aaagagcata cttggcggag aaaaaaagct ctttgcagaa
     4201 tcgtgtaatt tctgtaaagt agttttcaga cccagtcgtt cagaaaaagg tgccttctct
     4261 gcgaattcca gagtttcttc acggaaactg ttgtaaaacc tggaagaggc aaaacgagtc
     4321 aataattttg aaattgcacc aacatttgga tctatactga cgctgttatc attgtaaata
     4381 accagcatgt ttttatcgag agcaccattg tgattcagtg cttcaaaagc taaacctccg
     4441 gtcattgctc catctccgat aacagcaatg acacgatgtt ttttttgttg aagatcacgt
     4501 ccaatggcca ttccaagtgc tgcagaaatt gaagttgagg catgtcctgc tccaaaatgg
     4561 tcgaaactgc tttcgtcacg tgagagaaat tttttgatgc cgccggcttt accaagcgaa
     4621 tcaaattttt cattgcggcc ggtcagaatt ttataggcgt aggcctggtg tcctacgtcc
     4681 cagacaattc ggtcaatgtt gaaattaaaa tttttcagca atgctactgt gatttctaca
     4741 gtaccaaggg aagaagcaag atggcctcca cgtcggctgg tgactttgat gatacgttga
     4801 cggcattctt cggccaactg agttaattct tgcaaattta agttttgtat ctctgtaggt
     4861 tgtgagatac tttccaataa cgatttcatt cttgaatctt aaatacttag tttcaatggg
     4921 aaaaaatata aatgtcttca gatcttcagt tttatttccc aattggttca ggccactgat
     4981 gtaccatgtc tgtcagtacg aaaagataat catagggtac agtatatctt ggagggattc
     5041 ttatgtccac taatacactg gaacaattaa aacaggccaa atcggctctt gaggctgaga
     5101 gaaaacctgt atctcatgtt caggaagctg taaaggcaac aaaagatatc actcaatttg
     5161 ttaaaattgc actgagtaaa gaaaatcgct ggatttttca ggccggtgaa caggatagta
     5221 tagtttcaat tctggcggag atcaaccgaa ccaacaagga actgtataat aaatgccgca
     5281 ctccggagca ttttaataga gatgcaactc gttatttgaa aatgaagaat catataatcc
     5341 gcaagtctga gtgcatccat tttagtctgg atcaaggatt ttatgccaca gaaccacttg
     5401 gattttctgc cggaaaagca attgaattat ctctgaaaca aatccggaaa atacgcgacg
     5461 gtcaccagca aggaaaatgg acacgattgc caattgatgt aaatgtagat gattctgcaa
     5521 aaactgaagt tgaaaaaata tacgcagaga gcgcaccact tgcagaaaaa ttactcaaaa
     5581 atgaaaatat tggtaacttt gatccaccaa tgaccataga agaagttcaa tatgtttata
     5641 agtttttcca taatgttcac caaatccagg aattggagca gcaagcactg acggatgctc
     5701 tttcaaaaaa aacgcctgcg gaacagtcta ctccaaatga aattctggac caactttcca
     5761 gttttgaaaa aaagcttaaa aatgtaagag ttccgacctc aaaaaatatt ccggactggg
     5821 gtattcatct gtacgaaatg tcacttacgg atatgaccct ccgtatgcat ctgctccgta
     5881 tcccttttga accattggga tttcttaata gacatttgaa aatacttgaa gagcaaaaag
     5941 acagtgatgg tattgagaaa agtatctcaa gcttccatga taatgctgta caggaactgg
     6001 agaaactgca actttttagt gagtcttttg caggtgttga cgaaacttta caggaagtga
     6061 caggaattct acaaaacctt tcaatctgct ttggaaagat ttatcacaat gcatcttcac
     6121 aaactatcac caattctttc ctgatcaaag tccttgaact gatcaaaggt atgctggaaa
     6181 aaggtggtcg cgttgctgac tcaaagagcg atacattgct taaattacat caaagtgtca
     6241 atgatgttga cttgtttgat gacccaatta cagaaggaat gattcattcg ctggacttac
     6301 ttgaacaagg caaagaagcg atgagtcagt attgtgacaa acttaaaacc tttttggaaa
     6361 cactttcaaa tgcctctgac tgggactcca ggcagactta taaattcttg aagaacgctt
     6421 atgatgaaaa tgcgagttta acctcagcat caatgttttt tggaagtata actaaaacat
     6481 gtgttttgtt gaaaagttcc atcaaggaag tcgaatcaat gtggctggaa ctagctaagt
     6541 tgcgagaaca aaaacttgac tcaggggcct tgtatgagca aacaatagca ctgctgggac
     6601 aggacacggg attttctgaa aatagtttgc ggcaaagagc ggatcaagtg gaggagttga
     6661 attggatact ggatgaaatg ttaggtcttg gagtacaaga ggagcagcgt tttgtcgatg
     6721 aacttcattc agagttaata ccagtcagtg acaggattaa agctcttaga ataaaagaat
     6781 ctgcaagaaa agtagagttg acaagcacta aagaaaaatc agagcaagat tctattgtgg
     6841 taaaatccaa ggttagaaaa acaaaaaaat ctgataaatc cagtgagctt gctattggca
     6901 ataaaaaacc aacttacgat cctttccagc catttcaaat ggttgccctg aaaaaagcct
     6961 gcaaagaaat gacatcatcc gagggtaaaa tgatatatca ggtgttgaca aacacacaca
     7021 tatttttcac cctgttgaac aaaatggaaa gttccttcaa acaaggtgaa ggacttgaaa
     7081 atgaactgga acaggattta gctagaagca aaacaacaca ggaatttttg atgaattttg
     7141 caagattgaa atttttacca aatggttatt atagcgaaaa caataccccc tttgattaca
     7201 atgaagaaaa gaggcaattt gaggtcccgg aggggataaa aaaacagctt aaaattaccg
     7261 atagttctac cagttcaccc atggacattt tcagtcgact gattcgagtg ttgttgggtt
     7321 tagatgattc taatacgctt gctgccataa tgcccccgca aaatttatcc attatggagg
     7381 acgattttat gcttaaaaga aaagatcgaa aagccgttga ggaaggattg caatcgacgt
     7441 gaattccaaa aaagta
//
LOCUS       DTVD01000159           24930 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_289_length_24930_cov_335.482573, whole genome
            shotgun sequence.
ACCESSION   DTVD01000159 DTVD01000000
VERSION     DTVD01000159.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 24930)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24930)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24930
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_289_length_24930_cov_335.482573"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="EYO68_05520"
     CDS             <1..817
                     /locus_tag="EYO68_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07299.1"
                     /translation="MGIEYTWSESVKSADFARVMREYAENGYQLITGDSFGAERIARR
                     VARDYPQVSFVFGSGIGPAEPNFGVFDNWIHEPAYLSGMIAGKMTKTNILGVVAAMPI
                     PEVNRLSNAFCSGAKEVNKKVRCKFSFIGSFFDPPKAKEAALAQIEAGADVLYAERFG
                     VIEACKEKGVLAISNMSDQASLGPDTVITGPVWDMWPTIKHVVTLVKAGVPTAQDFGG
                     FSYMGKGGSYLAPYHNWDSKLPASVKAMVEKRKAEMLDGTFRADIDESAPKSD"
     gene            954..2471
                     /locus_tag="EYO68_05525"
     CDS             954..2471
                     /locus_tag="EYO68_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004140311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07300.1"
                     /translation="MSNPDYLLSMQGICKSFGAIQANNNVDLNLSEGDILGLLGENGA
                     GKTTLMNILFGTYQADEGKIMIQGRQITIHSTADALGFGIGMVHQHFHLVHRHTVLEN
                     LLVGEPGRRGRLDFSGARRRLLEIGDQFGLTLNPDARVSELTIGEQQRLEIIKALFGD
                     SKILILDEPTAVLTPNESTGLFEALRALAEGGLGIIFISHKLHEVRNVTNRVLVMRAG
                     SVSALVEDTKEVTESDLARLMCGHELNPPKKNTSKLGDTFLSLSAIKTGGSTPQKLKG
                     INLSVQGGEILGIAGVSGNGQKELADVIAGVLSHTEGTIEVKGEIISKASPRLMQKLK
                     IGRIPEDRMTTGLVTSLPLYVSMVLPWIRRSIFSRFGILRKNEILSFTSKMIKRFTIQ
                     ATGPEVKTGTLSGGNLQKVLLARELAFEPEVLLASQPTRGLDVAAAEFVHRQFLSLRE
                     KGAAIILISEDLEELFALSDRIVVMYEGRIAGETKINDASVEDIGLLMAGATFGA"
     gene            2501..3562
                     /locus_tag="EYO68_05530"
     CDS             2501..3562
                     /locus_tag="EYO68_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07301.1"
                     /translation="MAFRLELREKTPIWFNIVLPLSAVMATLLLCSGLVALAGANVFH
                     AYSELFLSAVSSRFNLVETFVKAAPLIFTGLAVAVAFRAKFWNIGAEGQLLAGAMAAA
                     YIGGIEFLPSFSLVPLMIIASALAGAGWAMLPAILRTRFKVDDVVTTLLLNFVMFYVM
                     TALLDGPWKDPLSGYPDSPDIRMDAEFPILLHATRLHLGVLLSLLAAPMVWWFIRRTT
                     LGFSISVVGENPSASAYAGISITRILIYTALISGSLAGLAGAGEVGGLHFQVMSGISP
                     GYGYTGIVIAMLARLNPIGVVPAALFFAVVVTGAEAMSRATGVPVYLADVIQGTALVT
                     MLIAQLFTTYRIRTLTTQG"
     gene            3566..4510
                     /locus_tag="EYO68_05535"
     CDS             3566..4510
                     /locus_tag="EYO68_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07302.1"
                     /translation="MEEIISQIFQVGFFAAIIRIATPFVFATIGELFAERSGVLNLGI
                     EGIMMFGAMTGFSTAYFTSSLWLGILAAMLTGMLMAALMGFLTVSLGLSQHVSGIGIT
                     MLSTGFAFFFYRLIFGQPSTLPNIKAFTPVGIPVLKDIPWIGPVLFEQTTLTYLAFAV
                     VPLAAFLLYKTPWGLNLRTVGENPHAADSAGVSVSAMRYQGLIISGALMGIAGAFLVM
                     VQFNAFTFGVISGRGWICIALVVFGQWGPWKSAAGALLFALIDALQLRLQASSIIDLP
                     YQVFLMMPFVLTIVTMMLVSRNARAPSALLIPFRKEER"
     gene            5013..6452
                     /locus_tag="EYO68_05540"
     CDS             5013..6452
                     /locus_tag="EYO68_05540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIC07303.1"
                     /translation="MAKNITSRKEDYSKWYLDVIAAGQLADYAPVKGCMVIRPTGYAI
                     WEAMQSRLDRMFKETGHVNASFPLLIPKHFMEREAEHVEGFSPECAVVTHGGGKELEE
                     PLMIRPTSETVIGHMYSQWIQSYRDLPVLINQWCNVMRWEMRTRLFLRTSEFLWQEGH
                     TAHETADEAQEETLRMLEIYRIFQEEYLAIPVITGLKSEAEKFPGALATYSIEAMMQD
                     GKALQSGTSHNLGQNFAKAFEIDYLDRNNNRKHVWTTSWGVSTRMIGGLIMTHSDDDG
                     LIIPPKIAPIQVVIVPIFNNDQERVQTFEFAEKISNSLNEKIDKLQIKIDTRDNLRPS
                     DKFFHWIQQGVPVRVEVGPRDVKKQSAMVVRRDVREKSAVPLENISEHIVELLERIQD
                     NLFQRALDYRQTNTRTAASYEEFKEIIADEGGFIFAHWDGSKEIEAKIKQETKATIRC
                     LPLEDRSETGKCMVTGKPSEQEALFAIAY"
     gene            6499..7728
                     /locus_tag="EYO68_05545"
     CDS             6499..7728
                     /locus_tag="EYO68_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIC07304.1"
                     /translation="MNKNIIVSNVSDESFALGVGYAHSQEIDISDLIALKSFINNEFC
                     PRFLQDHVTEETLGHGLKGKSVYIVSTHSAYYSRNELAMRNYLIASAAKENGAEFVAL
                     VEPDLFYSAQDRGPRTLDHPQVSDFASREKFVGQPCSAEMYAQLLKTSGVDSVMTVHN
                     HKPDVMRNIYQKVYPTGNSHKIPVFLNLDISPLIANYILRSGLVRLWNYGEHVGFVAP
                     DEGAVDFVKRVREFTGLHNSVLVTFKKTRMGQRDVKVDLSEDVELLKGKDIFILDDMV
                     RTGGTIAANINAISESKSCRPANIFFYSTHSTISPEARENLNSPHLNQFITSNTIPSV
                     LNRDIQGRLRKKIVVLKIEKWIANAIRHCLEEARYPDEIYGINSVTQSDDFYEVDLST
                     KNPLHNKSRVQQYELTI"
     gene            7731..>8089
                     /locus_tag="EYO68_05550"
     CDS             7731..>8089
                     /locus_tag="EYO68_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIC07305.1"
                     /translation="MSKDENTLILGIGGVGMHLAQRLVHEGYPVTVIESDSELLEAAA
                     ESLDARLICGNAMQLSSWREAHAQDMGLMIAATNDDSTNMLSSLIADRFGIERKIVRT
                     RSIDLMDGSILSPEDLK"
     assembly_gap    8090..8189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8190..8873
                     /locus_tag="EYO68_05555"
     CDS             <8190..8873
                     /locus_tag="EYO68_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIC07306.1"
                     /translation="MMILGGGLVGSRVAQLLEKEVEIKLIENNQNRADELASDLKNTE
                     VIHGDGTDANLLVMSGLDSTESFIATTGDNETNIISCLLAKHLMNRNNRDLQAGHGKT
                     IALVNKEDYLVLASTIGLDIALNAKISAANEILKFIRRNELLSVAHLHGVDAEVVELK
                     AGPGSEIARKPLKKLASYFRQHNILIGGVEQDGVWKVAVGSTQVQPHNRVVAVCSSHQ
                     LKKVRQLFN"
     gene            9048..9296
                     /locus_tag="EYO68_05560"
     CDS             9048..9296
                     /locus_tag="EYO68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07307.1"
                     /translation="MDQRLELQRICRAVSSRNTNRFMVLLGKIYPLNNDGRTAALEEI
                     STSLEDAGYFCGTYRFRNAPCSVGKNTLIDQTTCSYSR"
     gene            9508..10749
                     /gene="ftsA"
                     /locus_tag="EYO68_05565"
     CDS             9508..10749
                     /gene="ftsA"
                     /locus_tag="EYO68_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIC07308.1"
                     /translation="MPSNTKNNNLTVGLDIGTTKICAIAVEGDDIEALNVVGVGTAKS
                     DGLRKGVVVNIEKTVKAIKKAVEECELMCGAQIRSVFAGIAGHHIRGQNSRGMVTVYH
                     NRIVTDEDIRRVIDAAQVLIPNDREVLHILPQEFIVDDQDGVQNPLGMAAARLEVNVH
                     IVTGSVTSAQNIIKCCNQSGLDVEDIVLEPLSSSQAVLSPDEQEVGVVLVDIGGGTTD
                     VTIYSEGSIVHTAVLALGGNHLTHDIAIGLGAPLHEAEEIKHNFGVAMSSMVKEDEMI
                     DVPSVGGRNNRTMKKRVLASIIEDRFREIFELITHEIEKTHFHTLMASGVVITGGTCI
                     MPGADRLASQVLNLPVRVGYPENIAGLREMIYSPKYATSVGLVRYGITSNQGKLNFVG
                     DDTNLFHKVSRRMKDWMQDFF"
     gene            10807..12588
                     /gene="ftsZ"
                     /locus_tag="EYO68_05570"
     CDS             10807..12588
                     /gene="ftsZ"
                     /locus_tag="EYO68_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIC07309.1"
                     /translation="MLNFSDIQPVRDDLPKIKVVGVGGGGGNAVNRMIQSGVQGVDFV
                     VANTDLQDLRKSLAPQKLQIGSKCSRGLGAGAKPEIGRNAALESIDQIRDSLQGADMV
                     FLAAGMGGGTGTGGTPIVAQVAQEMKALTVGVVTLPFNFEAKRRRKVANGGVTELRDH
                     VDTLIVVPNENLFSIINRRTPMTEAFGFADDVLRQGVQGISDLITRDGLVNLDFADVR
                     SVMANKGKAVMGTGLASGENRARHAAEQALHSPLLNDNTIDGARGILINVVGGLMMGM
                     QEVEEASTFIKEQGHKDAEIIWGAAINPDFEDQMMITVIATGFDEREATEKIYVPGLP
                     AMPFDALVVTNKHSQINTETDEKEFNSEVELNLEKNDSIEPYSDDDYFEETESITAED
                     KVFVEGDPEDSSFSATAQSKDSNEESVVDDQEELSSHQISIHSSETESEEIITTEEIT
                     LTDESSHLSSDSEEEQLEEPESLMNETSTVESVNFSDSNLEVESGTFEDLAIDHSSTD
                     IDAENYSIHGFSGVSTKLNDNESNETHSFSVSNVTSENENDTETNPVETSPLIPEWAN
                     QTENPTFYQNLDIPTFLRRRGSNRPRQ"
     gene            12814..13689
                     /locus_tag="EYO68_05575"
     CDS             12814..13689
                     /locus_tag="EYO68_05575"
                     /inference="COORDINATES: protein motif:HMM:PF16697.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07310.1"
                     /translation="MKSTRSLHNAVLGLLGGLASWTLMQSGFHAFDALSVTGLSGLNI
                     IWLNKFIFEGALVGLGLGMVLQARVSLWYHYELVHILSKMFIGAFIGAILGLICFSIG
                     ELLQVWLVLPALSRIASWTLLGLLLVGTTELFHSRSGFLRPRIISGGIGGAIGGGIFE
                     LLLLYQMTGPDHLLGLILVGFSISLFVGITENRATSFALRVVSGKQEGQIFLLDQNRF
                     TLGYGSQNDFILKGYAEVCDLHANILKKGKEVIIENADEGGEVLVNYRLVDQQSMKKG
                     DVIKIGTALLQYYEI"
     gene            13689..14702
                     /locus_tag="EYO68_05580"
     CDS             13689..14702
                     /locus_tag="EYO68_05580"
                     /inference="COORDINATES: protein motif:HMM:PF16697.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIC07311.1"
                     /translation="MISQIDRCLRACVHSAGLLIIGILTSTSLPAAEQVVEQNVLIRE
                     GFHTAFLEYFFPQTFFLKINETPSLTLIESLLIFAGVLLAMFGLWFLQKPKAGTQSGV
                     GFQLLNLGERKPFIPLKSEIQTLEFLTQIQTSKKYRLSANLTRVTLTPHQNTFVLEDK
                     NYKNALLINRRRSHRTVLCDNDVLDVGEMILLFRNHVVPAGTIHRPASKDLQLPIVGI
                     IPKGPVHKMTPILTVSGSTQKIPLVRNLITIGSSNSNDIIVEGDEVALRHAKIYKVGA
                     SWKIQNLLIQETTTVNGRRIDQRFLQDGDEVTVGDIMFKFKSGKAQIKQTLKSKTETA
                     KKI"
     gene            complement(14708..16354)
                     /locus_tag="EYO68_05585"
     CDS             complement(14708..16354)
                     /locus_tag="EYO68_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07312.1"
                     /translation="MNKYRLSIFRIISTAFLFLGLLSAPLMGQSLLVEQAESTVEIKQ
                     DNLVRAKAEALKAAKGNVVLHAVARFLDFESMIYLKPLLIQQFFEHPDFYIESIRVIS
                     EGNTADLTEFTLNIETRIFHSRLLAAFRKLGLVTEQERIPFMDVYLIYDADHALRQTR
                     VLNLFLEQLQIRLRPYRIRTRVILTKGRKLPIEAGLPARLALLPLKSAEKRNGTNVAL
                     LELKLRLSPQPEKSPQGKLIAQLIFWSQKRDFSESSISTTMATAEISYKTWNTNVIIP
                     EILDQLLLQWTPVMQHIVTVNQVSGAQVKLKFKGLPGPIEEQQLIKTLFRNNPRWKKV
                     SLDTISSNFVSYKALFLGPLKQIIQEFRSPKNAPFRITSVNWEDNDLVVQVEWNEVPM
                     PLELFETTLTDDVFSEIDSEENYIPEPEYQVPLRTFKQTFKLPFAKAVYDHIRHRGDS
                     TLFRIEVPKNMNVNEENKLINLAWFRLGTTHLNPELTIFDQNRNRKKSYLLRRRKQFT
                     FQYRIPVGQEAFYLRISDEVGFLKGVAGSYQSFHYVLTAN"
     gene            complement(16365..18086)
                     /locus_tag="EYO68_05590"
     CDS             complement(16365..18086)
                     /locus_tag="EYO68_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIC07313.1"
                     /translation="MNIIEKVQFWAENPCFDAETQAEAKNLLAAENKNAREECFGSLL
                     EFGTGGLRGLMGVGTNRINRYTVQMATEGVARIIKNKKNESGAKGVVVGYDSRHFSFE
                     FAKVTSEVLAAHGIPVYLFRSIAPTPLVSCEILRRKAIAGIIITASHNPPEYNGYKVY
                     WENGGLIVPPEDSQIIEEVRNINRIDKIPAISFENALQNEIVEWIDNEADEYYLKTVR
                     KLSLGLRENNKDLGVIFTPLHGTGGRLVPELLQQQGFENVKVVKEQMVPDGDFSTVGS
                     PNPEDRDAFKMAVEQATDDEDLILANDPDADRLGVMVRDKEKQWHWLTGNQIGILILD
                     SIISALHQQNRFPKKGVIITTVVTSPLVSKMARENGLDLVEVLTGFKWIRQAALRYEE
                     EVDGKFVFGMEESHGYLMGSHSGDKDGIWASMAFAEMVATLKKSNLTAIDRLNQIHQQ
                     YGPHLDTLRTQTYTGTEGLEKIQQIMRKLRNSPPKSVAGQTVVKITDYLENTIICADA
                     TKNLQGPGLPKSNVISLELSDNTRITARPSGTEPKIKYYFNLHGKNSALLQERLVEIQ
                     AEFLSDQ"
     gene            complement(18120..19091)
                     /locus_tag="EYO68_05595"
     CDS             complement(18120..19091)
                     /locus_tag="EYO68_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIC07314.1"
                     /translation="MSIRMTSEHTELTFHYMRVEGREETLLEFLLRRFRYLDDQEWRE
                     NINDRRIWVDGKLGRTNFKLRNNQKIVYLRPDFLEPDVDLDFEIIFEDDALIAVCKSG
                     NLPTSPSGKYYKNTLVSLIKSQFGLKKLYTLHRLDRETSGVIIFAKRHEVAQSMAAHF
                     RKNRIHKIYSAILSRHLPQISKTTVPEIFISLPIGQDLNSNIRIKQSVNSLGKPSHTY
                     FREIGKMGDYSLVEVRPFSGRTHQIRVHAAHMGCSVVGDKLYGLPNDGFIRWLNEGDD
                     YLLSQNFPLHRQLLHAVEIRFPHPVTRKETVIRSDNEKILKELKQSS"
     gene            complement(19126..20169)
                     /locus_tag="EYO68_05600"
     CDS             complement(19126..20169)
                     /locus_tag="EYO68_05600"
                     /inference="COORDINATES: protein motif:HMM:PF14238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIC07315.1"
                     /translation="MFKPLLFTTFSLFIIGGGLIYYQDDNYFTKPQISKDNRLFPEQL
                     DTKALNQIKIESQGSSVNLMQLKGGGWKEQSLNYEADPIPIQDLLLNLSQIRLGDLVT
                     NNPDHHERFQLLTPPENKEDWSEDRHGFSVSLLRGDGTSILSFLLGKNRTSGPGQYVR
                     HKGSNKVFLIPEGLSIDSEANDWLKKDLLALESNLVQSLVLKNEEGGSFSINRDSAET
                     DWKSSSEKSDVPDSDKITTLLNRLGKLSFSKLYINDSVSQQAEGTDLIETTLFVSLFD
                     SRVYTLNLRNNIASNENYILSMRMGISLVASGGSEAEDSKLRDEMGIYNQQVNGRFYE
                     ISSWEGKELLLSE"
     gene            20441..23125
                     /gene="acnA"
                     /locus_tag="EYO68_05605"
     CDS             20441..23125
                     /gene="acnA"
                     /locus_tag="EYO68_05605"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIC07316.1"
                     /translation="MKDNQFGCRINFESSEGKTALYSLEKLQKDGIGTIDRLPFSIRI
                     LLEQALRNVDDFQVCAEDVTALANWNAAEKSNQEIPYKPTRVILQDFTGVPAVVDLAA
                     LRSAMVAMNGDPSVINPRIPVDLVIDHSIQVDAFGSSGALKKNMEIEFVRNRERYEFL
                     KWGKQAFDNFNVYPPGIGIVHQVNLETVATVVQSRDGVCFPDTLVGTDSHTTMINGLG
                     VMGWGVGGIEAESVMLGQPINMLIPEVIGFRLLGELQQRATATDLVLRVVEMLREKGV
                     VEKFVEFFGPGLNNLKLADRATLANMAPEYGATMGFFPVDDETLRYLRDTGRPDLLVQ
                     LVERYCKEQGLFRTSDTPVPEFSDTLELDLSTVEPALAGPKRPQDRVNLTQMQQNWQK
                     TLRVSRKERGFELSDEQIETSVQMQGIPSGKLKHGSVVIAAITSCTNTSNPSVMLAAG
                     LLAKKAVEKGLNRQPWVKTSLGPGSRVVTDYLEAAGLNEPLDALGFHTVGYGCTTCIG
                     NSGPLPESIVTAINQGDLVVASVLSGNRNFEGRISQHIKANYLASPPLVVAYAIAGTV
                     NHDLINEPLGNGKDGQPVFLKDIWPAQEEIGEVEKLITSEMYEKEYKGAGESSPLWNA
                     IEARTGEVYEWDDSSTYIQNPPFFQKMGLDVNALQDITGARVLAKLGDSITTDHISPA
                     GAFSSETPAGKYLLELGTEIKDFNSYGSRRGNDRVMTRGTFGNVRLRNELVPGTEGGF
                     TKLIPSGELTTIYEAAQKYKDSGTPLVVLAGAEYGTGSSRDWAAKGTLLLGVKAVVAT
                     SFERIHRSNLVGMGVLPLQFKNGETHKSLGLSGEEEYAVLGLTDNLKAGQDVILRADD
                     REITVTCRLDTLVELEFYKNGGILHTVLRNFMRESLRE"
     gene            23158..23709
                     /locus_tag="EYO68_05610"
     CDS             23158..23709
                     /locus_tag="EYO68_05610"
                     /inference="COORDINATES: protein motif:HMM:PF14279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIC07317.1"
                     /translation="MQDVLLLNQDGNPLTLWPLSTLSWQQAIKALYLDKVTVLRSYDD
                     WICHSQHLALPVPSVVMMSRYHQQQGTVNFTRRNIFLRDRFHCQYCLHQFSADHLTID
                     HVIPKSRGGGMRWKNVVAACHKCNLSKGANIVHPSQTPFEPNYWQMVKIAKTLHIKIP
                     HSSWQEYLQWPIHLVKIDQAKAA"
     gene            complement(23706..24794)
                     /gene="serC"
                     /locus_tag="EYO68_05615"
     CDS             complement(23706..24794)
                     /gene="serC"
                     /locus_tag="EYO68_05615"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIC07318.1"
                     /translation="MTNRVYNFSAGPATLPIPVMETVQKEFLDFHGLGASIVEISHRL
                     PVFKDIIETTKSLFRELTGLPNNYKVLFMHGGAQMQFSAVPLNLMSMKSHKGSYFITG
                     RWGTLAENEARKYGNTEILMDGKEFEYRQIPQYDTSSLDQDSSYAHLTTNNTLYGTRW
                     HQFPDTGNVPLVADATSDILSREMDYSQFGIVYAGLQKNLGTSGTGLVVVREDLLGHA
                     LPETPKLLDYALFDEHNSIPNTINVFAVYVMRLVLEWVKEQGGVPEMEKLAEKKSSLL
                     YEILDNSELYSSVAHPKHRSITNVTFHLPQETLLQKFLTETDKEGLFALKGHASVGGV
                     RASIYNAMPLEGVDALAQFMKEFERKNG"
BASE COUNT         7159 a         4831 c         5459 g         7381 t
ORIGIN      
        1 aatgggaatc gaatacacct ggtcggaaag tgtaaaatct gctgatttcg cccgtgtcat
       61 gcgtgaatat gccgaaaacg gttatcagtt gattacaggg gactcttttg gggcggaacg
      121 tattgctcgt cgtgtagcca gggactatcc acaggtttcg tttgtttttg ggtcaggaat
      181 cggtccggct gaacccaact ttggagtttt cgataactgg attcatgaac cagcctacct
      241 gtccgggatg attgccggaa aaatgaccaa aaccaatatc cttggagtcg ttgcagccat
      301 gccgattccg gaagtgaacc ggctttcaaa cgctttttgc agcggagcca aggaagtgaa
      361 taaaaaagtg cgatgcaagt tctctttcat tggttcgttc tttgatccgc ctaaagccaa
      421 agaagcagct ctggctcaga ttgaagcagg tgcagacgtg ctttacgctg agcgttttgg
      481 tgtcattgaa gcctgtaaag agaaaggtgt gctggccatt tctaacatgt ctgaccaggc
      541 ttctctagga ccagacacgg tgattaccgg tcctgtctgg gacatgtggc ctaccattaa
      601 gcacgtggta acgctggtca aagccggagt accgacagcc caggatttcg gaggattttc
      661 atacatgggc aaaggagggt cttatcttgc gccctatcac aattgggaca gcaaacttcc
      721 tgcgtctgtg aaggctatgg tggaaaaacg taaagcggaa atgcttgacg gtaccttccg
      781 ggctgatatt gacgagagtg ctcctaaatc agactgatta cttttacccg actcaacggt
      841 tttccgttat ttataacgga aaaccgccat tcaggctatt cctcatcatt aaggaacgtt
      901 aaacgttcat agtcttccat ttcactactg tatttctgtt ccttcccctt gttatgtcca
      961 atccagatta tttgctgagt atgcagggca tctgcaagag ttttggagca attcaggcaa
     1021 ataacaatgt tgatctcaac ctcagtgaag gagacatcct tggtcttctg ggtgaaaatg
     1081 gtgcaggcaa gactacactg atgaacatcc tgttcggtac ctatcaggcc gatgagggaa
     1141 agataatgat ccagggcaga cagatcacta tccattcaac tgcagacgca cttggatttg
     1201 ggataggaat ggttcatcag cacttccatc tcgtccatcg acacacagta ttggaaaact
     1261 tattggtagg cgaacccgga agaaggggaa gactggattt ctccggagcc cgcagacgtc
     1321 ttttggaaat aggagaccag tttggtttaa cattgaatcc tgatgcaaga gtttctgaac
     1381 ttacgattgg tgaacagcaa cgtcttgaaa tcatcaaagc ccttttcgga gactccaaga
     1441 ttcttatcct agatgaaccg actgcggttc tgacacctaa tgaatctaca ggtctttttg
     1501 aggcactccg cgctcttgcg gaaggagggc tgggcattat tttcatcagt cacaaacttc
     1561 atgaagtcag gaatgttacc aaccgggtgc ttgttatgcg cgccggttct gtgtctgcac
     1621 tcgttgaaga tacaaaagag gtaacagaaa gcgatcttgc ccgtttgatg tgtggtcatg
     1681 aactgaatcc tccaaagaag aatacctcca aactcggtga tacttttctg agtctttcag
     1741 caattaaaac aggtggttct actcctcaga agttgaaagg gatcaacttg agtgttcagg
     1801 gtggagaaat tcttggtata gccggagttt caggaaatgg tcagaaggaa cttgcggatg
     1861 tgattgccgg agtgctttct catacggaag gaaccattga ggtgaaaggt gaaattattt
     1921 ccaaagcaag tcccaggtta atgcagaaat tgaaaatagg acgcatcccg gaggatcgta
     1981 tgacaacggg gctggtaacg agcctgcccc tttatgtgtc catggtactg ccctggatca
     2041 gacgttctat tttcagtcgt tttgggattt tgcggaaaaa cgaaatcctg tcctttacca
     2101 gtaagatgat taaacgtttc accatccagg cgacaggtcc ggaagtgaaa acaggaactc
     2161 tttccggagg aaacctgcag aaagttctgc tggcacgtga actggccttt gaaccagagg
     2221 tattgctggc atcccaaccg acgcgtggac tggacgtggc agccgcggaa tttgtccatc
     2281 gacagtttct ctctttacgt gaaaaaggag cagcgatcat actcatcagt gaagatctgg
     2341 aagaactgtt tgcattgtca gaccgcattg tcgtgatgta cgaaggtcga attgctggag
     2401 agacaaaaat taatgatgca tcagtagagg atattggtct gttgatggca ggcgccactt
     2461 ttggagcata atacaatgat gaattaatag atcagttatc atggcctttc gactcgaact
     2521 cagagaaaag acaccaatct ggttcaatat tgttcttcct ttatcggcag ttatggcgac
     2581 attgctcctt tgcagtggac ttgtggctct tgcaggtgcg aatgtttttc atgcctactc
     2641 agaacttttc ctgagtgctg tttccagccg cttcaatctg gttgagactt ttgtaaaggc
     2701 agctcccttg atctttacag gactggcagt tgcggtcgct ttccgtgcaa aattctggaa
     2761 tataggagcg gaagggcagt tgcttgccgg agcgatggcc gcagcttata ttggaggtat
     2821 tgaattcctg ccctcgtttt cactggttcc tctgatgata attgcatctg cactggcagg
     2881 tgcaggatgg gccatgttgc ctgcaattct gcgtacacgc ttcaaggtgg acgatgtggt
     2941 cacgacgttg ctgctaaatt tcgtcatgtt ctatgtgatg accgcacttc tggacggtcc
     3001 ctggaaggat ccactgagtg gttatcctga ttcaccggac attcgcatgg atgccgagtt
     3061 tcctattctt ttgcacgcta cccgtttgca tctgggtgta ctgctctctt tgctggcagc
     3121 accaatggtc tggtggttca ttcgaagaac taccctggga ttttcaataa gtgttgtagg
     3181 agaaaaccca tcagcatcag cttacgcagg aatctcaatt accagaattc tgatatatac
     3241 cgcattgatc tccggctcac ttgccgggct tgccggtgca ggcgaagtcg gaggacttca
     3301 ctttcaggtg atgtccggta tttcacccgg ttatggttat acaggtattg tcattgccat
     3361 gctggccaga cttaacccca ttggagtagt acccgcggca ttgttttttg cagttgtggt
     3421 gactggtgca gaagctatgt cacgggccac aggcgtacct gtttaccttg cagatgttat
     3481 ccagggcaca gccctggtaa caatgctgat tgcacagttg tttaccactt accgcattcg
     3541 aactctaaca actcagggtt gaaatatgga agaaattata tctcagattt tccaggtagg
     3601 attttttgca gcaataatca gaattgccac tccgtttgtg tttgcgacga tcggggaact
     3661 ttttgcagag cggtccggag tacttaatct tggtattgaa ggtataatga tgtttggagc
     3721 aatgacaggt ttttctactg cttatttcac cagcagcctc tggttgggaa tcctggctgc
     3781 tatgctcaca ggaatgttga tggcagcatt gatgggattt ctgacagtca gtcttggact
     3841 gagccagcat gtttcgggaa ttggaataac tatgctttcc actggttttg cttttttttt
     3901 ctaccggctc attttcggcc agccgtcaac tcttccaaat atcaaagcat ttacaccggt
     3961 tggcattcct gtgctcaagg atattccatg gatagggcca gttctttttg aacagacgac
     4021 attgacctat ctggcctttg ctgtagttcc tctggcggcc tttcttctgt ataaaactcc
     4081 atggggcctt aatctgagga cggttggaga aaatcctcat gcagccgatt cagcgggtgt
     4141 aagtgtatct gcgatgcgtt accagggtct tataatatcc ggtgcattga tgggaattgc
     4201 gggcgctttt cttgtgatgg tacagttcaa tgccttcact ttcggtgtga tttcggggcg
     4261 cggttggatc tgcatcgccc tggttgtctt tgggcaatgg gggccgtgga agagtgccgc
     4321 cggagctctc ctttttgcgc tgattgatgc tcttcagctt cggcttcagg ccagcagcat
     4381 tattgatttg ccttaccagg ttttcctgat gatgcctttt gtgctgacca ttgtaacaat
     4441 gatgttggtt tccagaaatg cccgagctcc ctcggcactg ctcattcctt ttcgtaaaga
     4501 agaacgctga acaagcagat ataaatagtg tttgaactat aataataatc agtagtaata
     4561 acagttaatt attcgtgatt tctgaaattc agacttaaca aaaaagctgt aagttgtcag
     4621 ctaaaaaaat attggccaga ttgttcaaac tgaaaacaga tagctgaaaa ctatcgtcca
     4681 aatctttgtt tatttaaaac tttctgaaac ggtaaaaaaa ctttgccgga cagattttcg
     4741 ttcagagact agataagaag acaagaaatt tctttctgaa aaaagaaaaa cagtatttac
     4801 atgtaattag tttgaaatca ggattctaaa ttccttttta atttcatcaa aatattttta
     4861 acttcatttc aataacatct tgtatctgta ctataaagtt tgtaactttg tagtctcaat
     4921 ccgacacggt caactcaggc tgacaaaact atttaaacgt tatgtgttgg aactgaagcc
     4981 ccttttgctt tttccaaaaa aaatctgccc aaatggcaaa aaatattaca tctcgcaaag
     5041 aagactactc caagtggtat ctcgatgtga ttgcagcagg gcagcttgcc gattatgcgc
     5101 cggtaaaagg ctgtatggtg attcgtccca cgggctatgc catttgggaa gccatgcagt
     5161 ctcgtctgga ccgcatgttc aaagaaacgg gtcatgttaa tgcctcattc cctttattga
     5221 tccctaaaca ttttatggaa cgtgaagccg agcatgttga aggattcagt cctgaatgtg
     5281 cggttgttac gcatggagga ggaaaagaac tggaagaacc gctaatgatt cgtccaactt
     5341 ccgagactgt aattggtcac atgtattcgc agtggattca aagttatcgt gatcttccgg
     5401 tgttgatcaa tcagtggtgt aatgtgatgc gctgggaaat gcgcacccgt ttatttttgc
     5461 gtacctcaga gttcctttgg caggaaggtc acacagctca tgaaactgcc gatgaagccc
     5521 aggaagaaac attgcggatg ctggaaatct accgtatttt tcaggaagaa tatttggcga
     5581 ttcctgtgat tacaggttta aaatctgaag ccgaaaaatt tcctggagca ctggctacgt
     5641 attcaattga agcaatgatg caggacggta aagccctgca atccggaact tcacataatt
     5701 tgggtcaaaa tttcgcaaaa gcatttgaga ttgattatct tgatcgaaac aataatcgaa
     5761 aacatgtttg gacaaccagc tggggtgtat caacacgaat gatcggcggt ttgattatga
     5821 cacatagtga tgatgacggt ttaattattc cgccaaaaat tgctcccatt caagtagtaa
     5881 ttgtgcctat ctttaataat gatcaggaac gtgttcaaac atttgagttt gcagaaaaaa
     5941 tttcaaacag cctcaatgag aaaattgata agctccagat aaaaattgat actcgtgaca
     6001 acctgcggcc ttcagacaag ttttttcact ggattcagca gggtgtaccg gtgagggttg
     6061 aagttggtcc tcgcgacgta aaaaagcagt cggcaatggt agtgagaagg gatgtaagag
     6121 aaaaaagtgc agtaccgctt gaaaacattt ctgaacatat tgtagagttg ttggaaagga
     6181 tacaagacaa cttgtttcaa cgtgccctgg actatagaca aaccaatacc cggactgctg
     6241 caagttatga agaatttaag gaaattattg ctgatgaagg tggattcatt tttgcgcact
     6301 gggacggttc aaaggaaatt gaagcaaaaa taaaacagga aacaaaggcg actatccgtt
     6361 gtctcccact tgaggataga tctgagacag gaaaatgtat ggtaaccggt aagccctctg
     6421 aacaagaggc actgtttgca attgcttact gaagagcatg caaaaaaccg aagaaaatct
     6481 taaaccattc aaatttttgt gaacaagaat atcattgtat caaacgtcag tgacgaatct
     6541 tttgcgcttg gagtcggcta tgcgcattct caggaaattg atatttctga tttgatcgcc
     6601 ctgaaatcat ttataaacaa tgagttttgc ccaagatttt tacaggatca tgttacagaa
     6661 gaaactttgg gacatggact aaagggcaaa tcagtttata tcgtgtcaac tcattctgcg
     6721 tattattcgc gaaatgaact ggcaatgagg aattatttaa ttgcatctgc agcaaaggag
     6781 aacggagcag agtttgttgc tttggttgaa ccggatttat tttattcagc ccaggaccgt
     6841 ggaccaagaa cgctggacca tccgcaagtt tccgattttg cttcacgtga aaaatttgta
     6901 ggacaacctt gttctgcaga aatgtatgcg caactgctga aaacatccgg ggttgattcg
     6961 gtgatgactg ttcacaacca taaacctgat gtgatgcgga atatatatca aaaagtttat
     7021 ccaactggca attcccacaa aattccagtt ttcctgaatt tggacatttc gccactcatt
     7081 gccaactata ttctccgttc tggactggtc cggctttgga attatggaga acatgtagga
     7141 tttgttgcac ctgacgaggg agcggtcgat tttgttaaac gtgtgagaga atttacgggc
     7201 cttcataact ctgttttggt gactttcaaa aaaacacgca tgggtcaacg agacgtgaaa
     7261 gttgatttaa gcgaagatgt agagttgctg aaaggcaaag atatttttat tctcgatgat
     7321 atggtacgta caggtggaac aattgcggca aatatcaatg ctatttcaga gtcaaaaagt
     7381 tgccgacctg caaatatctt tttctacagc acacattcaa caatctctcc ggaagctcgt
     7441 gaaaacctga attctcctca cctgaatcaa ttcataacga gcaacacaat tcccagtgtt
     7501 cttaatcgtg acattcaggg acgtcttcgt aagaaaattg tggtcttgaa aattgaaaaa
     7561 tggattgcca atgcaattag acattgtctg gaagaagcca ggtatccgga tgaaatatat
     7621 ggaatcaatt ctgttactca atctgacgac ttttacgaag ttgacctctc aacaaaaaat
     7681 cctctgcata acaagtcccg agttcaacaa tacgagttga caatctgaat atgtctaaag
     7741 acgaaaatac gcttatcctt ggaattggcg gagtgggaat gcacctcgct caacgattgg
     7801 tccatgaagg ttatccggta actgtaatcg aatccgattc tgaattactt gaagcagcag
     7861 cagagtcact tgacgcccgt ttgatatgtg ggaatgccat gcaactttca agttggcgtg
     7921 aggctcatgc acaggatatg ggtctaatga ttgctgccac aaatgatgat tccactaata
     7981 tgttgtcttc cttaattgct gacagatttg gcattgagcg aaaaattgtc cggacgcgtt
     8041 ctattgattt aatggatgga agtattttat ctccggaaga cctgaagatn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgatgattct cggaggcggc ctggtaggaa
     8221 gtagagtagc acaactgctg gaaaaagaag tggagataaa gctcatcgaa aataaccaga
     8281 atcgtgcgga cgaacttgca tctgacttga aaaatacaga ggttattcat ggtgatggca
     8341 cagacgcaaa tttacttgtt atgtcaggac ttgattctac cgaatctttc atcgcgacaa
     8401 ctggagataa tgaaacaaat ataatcagct gtcttttggc caaacatttg atgaatcgaa
     8461 ataatcgtga tcttcaggct ggtcacggta agacaattgc gttggttaac aaggaggact
     8521 accttgtttt agcatctaca attggattgg atatagctct caacgcaaaa atttctgcgg
     8581 ccaacgaaat cctcaaattt atccgcagaa atgaactctt gtcagtggcc catctgcatg
     8641 gagtagatgc ggaagttgtt gaactgaaag caggacccgg ctcggaaatt gcacgtaagc
     8701 ctttgaaaaa attggcgagt tactttcgtc agcataatat tttgatcgga ggtgttgagc
     8761 aggatggagt ttggaaagtc gctgtgggtt caactcaagt acaacctcac aaccgtgtag
     8821 ttgccgtttg ttcctcacat caactaaaaa aagtaagaca gttatttaat tgaaataatt
     8881 agttttaata ccccctcaaa ctttttaata attattttca tataatccta ccaatataca
     8941 atgaatgggt atatactatt tcaatggaat tgctgttaag atgtttaaca gggctgttta
     9001 tccttatttc agttggatgt agccaagagc gaaggtatgt gtatcccatg gatcaacgtc
     9061 ttgagttaca aaggatatgt cgagctgtaa gttccagaaa tacaaatcgt ttcatggttt
     9121 tattgggtaa aatttacccc ctgaataatg acgggcgcac agcagcactt gaagaaataa
     9181 gtacaagttt ggaagatgct ggatattttt gcggaactta tcgctttaga aatgcacctt
     9241 gttcagttgg aaaaaacaca ctgattgatc aaacaacgtg ttcttattca aggtaaataa
     9301 ttgaaaatat tagcttattt taacaattta ttttatacga taaaatgtta tagagaaata
     9361 aagaaaatat tcacttctat gaaaatcatt aaaaccctgt ctgaatgcag ttttaacatg
     9421 gacagtaaat tgcacccaaa ggtagtttca ttattttcat tcataatttc tgcaattagt
     9481 acgaaaattt aaagacgagg agcatggatg ccgtcgaaca ctaaaaacaa taacttgacc
     9541 gtaggacttg atattggaac cacaaaaatc tgtgcaattg cagttgaagg tgatgacatt
     9601 gaagcattga atgttgttgg agtcggcaca gcaaaatcag acgggttacg taagggtgtc
     9661 gtcgtcaata ttgaaaagac agtcaaggca atcaaaaagg cagtagaaga atgtgaatta
     9721 atgtgtggag cccagattcg ttccgtcttt gctggcattg caggacatca tatcagggga
     9781 caaaacagca gaggtatggt cactgtttat cataaccgta ttgttactga tgaagacata
     9841 cgtcgtgtca ttgacgcagc ccaagtattg attcccaatg atagagaagt gctgcacata
     9901 ctcccccagg aattcattgt tgatgaccag gatggcgttc aaaaccctct tggcatggct
     9961 gcagcacgtt tagaggtaaa tgttcacatt gttactggtt cagtcacctc tgcacaaaat
    10021 attattaaat gctgcaatca gtccggactg gatgtagagg acattgtact ggaacctttg
    10081 tcctcaagtc aggcagtcct cagtcctgat gaacaggagg tcggagtggt actggttgat
    10141 attggcggag gtacaacaga cgtcactatt tattcagaag gcagcattgt ccacacggct
    10201 gttttggcat tgggcggcaa tcatttgaca cacgatattg ccatcggttt aggtgcacct
    10261 ttacatgaag cagaggaaat taaacacaat tttggtgtgg cgatgtcctc catggtgaaa
    10321 gaagacgaaa tgattgatgt ccccagcgta ggcgggagaa ataaccgcac catgaaaaaa
    10381 cgagtgttgg caagtatcat cgaagatcgt tttagggaaa tatttgaatt gattacacat
    10441 gaaattgaga agacccactt tcatacattg atggcatcag gtgtagttat tacgggtgga
    10501 acctgtatta tgccgggtgc agaccgactg gcttctcaag ttttgaattt gccggtacgg
    10561 gtgggatatc cggaaaatat tgctggttta cgtgaaatga tttattcacc gaaatatgca
    10621 accagtgtag gacttgtacg ttacggaata acaagcaatc aaggaaaatt gaattttgta
    10681 ggtgatgaca caaatttatt tcataaagta tccagacgta tgaaagattg gatgcaagat
    10741 tttttttagt tttttgacag gtcaatatca aacatgatct ctatataaca accaaaggga
    10801 gcaaacatgc taaatttttc tgatatccaa cccgtccgtg acgatttacc gaaaataaaa
    10861 gttgtcggtg taggcggcgg cggcggcaat gcagtcaatc gcatgataca atcgggtgtt
    10921 caaggggtag attttgttgt agcaaataca gatttacaag atttacgtaa atcacttgct
    10981 cctcagaaat tgcagattgg cagcaaatgc tcccgaggtt taggggcagg tgctaaaccg
    11041 gaaattggcc gtaatgctgc gttggaaagt attgatcaaa taagagattc ccttcaaggt
    11101 gcagatatgg ttttcctggc cgcaggcatg ggcggtggaa ctggaactgg aggaacacca
    11161 atagttgctc aggtagctca ggaaatgaaa gcccttactg ttggtgttgt gacattgccg
    11221 tttaattttg aagcaaaacg acgtcgaaaa gtcgcaaatg gaggtgtaac tgaattgaga
    11281 gatcatgttg acacgcttat agttgtacca aacgaaaatt tatttagtat catcaatagg
    11341 cgaaccccga tgaccgaggc ctttggattt gcggacgatg tgcttcgtca aggagttcaa
    11401 ggtatatctg atttaataac aagagatgga ttggtcaatt tggattttgc agatgtccgg
    11461 tctgttatgg caaacaaagg gaaggcagtt atggggaccg gacttgcctc aggggaaaat
    11521 agagcgagac atgcagcaga acaggctctc catagccctt tgctgaatga taacacaatt
    11581 gacggcgcac gcggaatatt gattaatgtt gtaggaggac tgatgatggg gatgcaggag
    11641 gttgaggaag catctacatt cataaaggaa caaggacaca aagatgctga aataatttgg
    11701 ggtgctgcaa taaatccgga ttttgaagat caaatgatga ttacagtcat tgcaacgggt
    11761 tttgatgaga gggaagcaac agaaaaaatt tacgtcccag gtcttcctgc aatgcctttt
    11821 gatgcacttg ttgtgacaaa taaacattcc caaataaaca cggaaacaga tgagaaagag
    11881 ttcaattctg aagttgaact taatctggaa aaaaatgatt ccattgaacc atacagtgat
    11941 gacgattact ttgaagaaac tgaatcaatt actgcagaag ataaagtttt tgttgaaggt
    12001 gatcctgagg acagctcttt ttcagcaact gctcaatcta aagactcaaa tgaagagtca
    12061 gtagtcgatg atcaggaaga actttcaagt caccagatat ctatacattc atcagaaaca
    12121 gaaagtgaag aaattatcac aactgaagaa attacattaa cagatgaatc atcacatctt
    12181 tcaagtgaca gcgaagaaga acagttggaa gaaccggagt cattgatgaa cgaaacttct
    12241 acagttgaat ctgttaattt ttctgattca aatctggaag ttgagtcagg cacatttgaa
    12301 gatttagcaa tagatcattc ttctacagac attgatgcag aaaactattc catccatggt
    12361 ttttcaggag tgtcaacaaa actgaatgat aatgaatcaa atgagacaca ctctttttca
    12421 gtttctaatg tcacttcaga aaatgaaaat gatactgaaa ccaaccccgt tgagacaagc
    12481 ccgctgatac ctgagtgggc aaatcaaacg gaaaatccca cattttatca aaatttagac
    12541 attcctactt ttctgagaag aagagggtca aatcgaccac gccaataaaa taacttgtag
    12601 ttctatttgt ttcacattat tctagcacct gaggctgaaa actgagcggt tagcagatta
    12661 tatagatatt tttgaaacag cacaagtacc aaccgtgagt aattacctgt cggcttcaga
    12721 catatttttc caagtttttg tagagaagag ttgctaattt attttgattg aattagtggt
    12781 tctaaaatta atttttcatt tagtatttta cttatgaaat caactcgcag cttacataat
    12841 gcagtgttag gtcttctagg aggtcttgca agctggacgt taatgcaaag cgggtttcat
    12901 gcgtttgatg ctctttccgt tacaggacta tcaggattaa atattatttg gctcaataag
    12961 tttatttttg aaggggcttt agtcggtctc ggactgggca tggttctaca ggcaagggtt
    13021 tcactttggt accattatga attggtacat attttgtcca aaatgtttat cggtgctttc
    13081 attggagcga ttctgggact aatttgtttc agtatcggcg aattattgca agtttggctg
    13141 gttttgccag cattaagccg tatagcaagt tggacacttt taggattatt attagttgga
    13201 accactgagt tgttccattc ccgttctggt tttttaaggc cacgaatcat tagtggagga
    13261 attgggggag caatcggagg tggaattttt gaattattgc tgctgtatca gatgactggt
    13321 ccggatcacc ttttaggatt gattctggtt ggtttcagca tctcattatt tgttggaatt
    13381 actgaaaatc gtgccacttc ttttgctctt cgagttgtgt cgggcaaaca agaaggacaa
    13441 atatttctac ttgatcaaaa tagattcact ttaggatatg gttcacaaaa tgacttcatt
    13501 ttgaaaggtt atgcagaagt ttgtgacctt catgcaaaca ttcttaaaaa aggcaaagaa
    13561 gtcattattg agaatgcaga cgaaggaggt gaagtcctgg tgaattatcg tctggtcgat
    13621 caacaatcca tgaaaaaagg agatgtcatc aaaattggta cagcgctgct gcagtactat
    13681 gaaatctgat gatctcacaa atagatcgat gtcttcgtgc ctgcgtgcat agcgctggac
    13741 ttttaattat tggaatcctc acatcaacat ctttgccggc tgcggaacag gtcgttgaac
    13801 aaaatgtgct gattcgggaa ggctttcaca ctgcgttttt agaatatttc tttccgcaga
    13861 cttttttcct taaaattaat gaaaccccca gtctgactct tatagagtcg cttctgatat
    13921 ttgcaggagt acttttggcg atgtttggat tatggttcct gcaaaaaccg aaagctggaa
    13981 cacaatccgg cgtgggtttt caacttctca atcttggtga acgaaaacca ttcattcctc
    14041 tgaaatcaga aatacaaaca ctggagtttc ttactcagat tcaaacaagc aaaaaatatc
    14101 gtttgtctgc aaatttaacc cgggtgactc taactccaca tcaaaacact tttgtacttg
    14161 aagacaagaa ctacaagaat gcactactga tcaatcgaag acgttctcac cgaacagttc
    14221 tgtgtgataa cgatgttttg gatgttggtg aaatgatttt acttttccga aaccatgtag
    14281 ttcctgcagg tacgattcac cgtccagcaa gtaaagattt acaacttcca attgttggaa
    14341 ttattcccaa aggacccgtt cataaaatga caccaatttt aacagtttca ggaagtacac
    14401 agaaaattcc tttagtacgt aacctgatta ctataggatc atcaaatagt aatgatatta
    14461 tagttgaagg tgatgaagtg gccttgaggc acgcaaaaat atataaggtt ggagcatcct
    14521 ggaagattca aaacctgctc attcaggaaa ctactacagt taacggacgt cgaatagatc
    14581 aacgttttct gcaggacggc gatgaagtta ctgttgggga cattatgttt aagtttaagt
    14641 ccggtaaagc acaaataaag caaactctta aatcaaaaac agagactgcc aaaaagattt
    14701 gaaatactta atttgctgtc aaaacataat gaaaagactg gtagcttcct gcaacgcctt
    14761 ttaggaaacc cacttcatca gaaatgcgca gatagaaagc ctcttgtcca actggaattc
    14821 tgtactgaaa tgtaaattgt ttccgccttc gtagtagata tgatttttta cgatttcggt
    14881 tttggtcaaa tatcgtaagt tccggattta aatgagtagt accaagacgg aaccaagcta
    14941 agtttatgag tttattttct tcatttacgt tcatgttttt gggaacttct atcctgaaca
    15001 atgtactgtc tccacgatgc cgaatatgat cataaacggc ttttgcaaag ggtagtttga
    15061 atgtttgctt gaatgtgcgt aagggtactt gatattccgg ttcaggaata tagttttctt
    15121 ctgagtcaat ttcagaaaat acgtcatccg ttaaagttgt ttcaaatagt tccaggggca
    15181 taggaacttc attccattcc acctgcacaa ccaaatcatt atcttcccaa ttgactgagg
    15241 taatccgaaa aggtgcattc ttaggtgaac ggaactcttg tataatctgc ttcagtggcc
    15301 ctaaaaaaag agctttgtaa gaaacaaaat tactggaaat agtatctagg ctgacttttt
    15361 tccagcgggg gttattccga aataaagttt taattaattg ctgctcttct attggtcccg
    15421 gtaatccttt gaattttagt tttacttgag ctccgcttac ctgattcaca gttacaatgt
    15481 gctgcataac aggcgtccat tggagtaaca attgatcaag aatttcggga ataattacat
    15541 ttgtgttcca ggttttatag gaaatttctg cagttgccat ggttgtactt attgatgatt
    15601 ccgaaaaatc ccgcttttgt gaccagaaaa taagttgtgc tatgagcttt ccttgaggtg
    15661 atttttcagg ttgcgggctc agcctaagtt ttagttcaag taatgctaca ttagtcccgt
    15721 ttcttttctc agcagatttt agtggcaaca atgctagtcg agccggtaga cctgcttcaa
    15781 tcggtaactt gcggcctttt gttaggatta ctctggttcg aattcgataa ggtctcagcc
    15841 gaatttgcag ttgttctaaa aacagattca aaactcttgt ttgacgtaat gcgtgatccg
    15901 catcatagat tagataaaca tccatgaatg gaattcgttc ttgttctgta actaatccta
    15961 atttgcggaa tgctgccagt aaacgtgaat gaaaaattcg tgtttcaata tttaatgtaa
    16021 attcagtcag gtctgcagtg ttgccctcac tgattacacg aatagattca atataaaaat
    16081 cgggatgttc aaaaaactgt tgaatcaaaa gtggctttaa ataaatcata ctttcaaaat
    16141 caagaaacct tgcaactgca tgcagaacaa cattcccttt tgcagctttc agtgcctcag
    16201 cttttgctcg taccagattg tcctgcttaa tttcaacagt tgattctgcc tgttcaacaa
    16261 gtaaactctg ccccatcaaa ggagcagaaa gaagtcccag gaacaggaat gctgtagaaa
    16321 ttattcgaaa aattgacaat ctgtatttat tcatttaatc atgttcattg atcgctgaga
    16381 aattctgcct gaatttcaac aagacgttcc tgtaaaagcg cagaattttt accatgtaaa
    16441 ttaaagtagt atttaatttt tggctcagta ccggaaggtc gagcagtaat tctggtattg
    16501 tcggacaatt ccagtgaaat cacatttgac ttaggtaatc ccggcccttg caaattctta
    16561 gttgcatctg cacaaattat agtattttct aaataatcag ttatttttac tacagtttga
    16621 cctgccacag atttcggagg agagttgcgt aacttacgca taatctgctg tattttttcc
    16681 aaaccttctg tgccggtata agtttgcgtc ctcagagtgt cgagatgtgg cccatattgt
    16741 tgatgaattt gattaagcct gtcgattgca gtcaagttac ttttcttcag tgtagccacc
    16801 atttcagcaa aagccataga ggcccaaatt ccatctttat ctcccgaatg gcttcccatc
    16861 aaatagccgt gactttcttc catgccaaaa acaaactttc catcaacctc ttcttcataa
    16921 cgcaaggcag cttgtctaat ccatttaaat cctgttaaca cttcaacgag atccaatcca
    16981 ttttcccggg ccatttttga gacaagagga gaggtcacta cagttgtaat gattactcct
    17041 tttttcggaa agcgattttg ttggtgcaat gcagaaatga ttgagtctag aatcaatatc
    17101 ccaatttggt tgcccgtcaa ccaatgccac tgcttctctt tgtcacggac cataacgccc
    17161 agacggtccg catcaggatc attggctaag ataaggtctt catcatcggt tgcttgttcc
    17221 actgccattt taaaggcatc cctgtcttca ggattgggtg atccaactgt gctgaaatct
    17281 ccatccggaa ccatttgttc tttgacgact tttacatttt caaagccttg ctgttgtaac
    17341 aattctggaa ccaaacgacc gccggtaccg tgaagcggag taaaaatcac acctaaatcc
    17401 ttattatttt cacgcagccc caaacttaat tttctaacgg tcttcagata atattcgtct
    17461 gcttcattat caatccactc tactatttca ttctgaagtg cattttcaaa agatatcgct
    17521 ggaattttgt caatacggtt gatattccgg acttcttcta taatctgact gtcttccgga
    17581 ggaacaatca aacctccatt ttcccaataa accttgtaac cgttgtattc cggtggatta
    17641 tgactggcag tgataataat tccggctatt gctttacgcc tgagaatctc acaggaaacg
    17701 aggggggtag gtgcaattga acggaacaaa taaacaggta tgccgtgtgc tgctaaaact
    17761 tcgctggtta cttttgcaaa ctcaaacgag aagtgacgtg aatcgtaacc aacgaccaca
    17821 ccttttgcac cggactcatt tttcttattt tttataatac gtgcgactcc ttctgttgcc
    17881 atttgcacag tatagcggtt gattcgattt gtaccaacac ccattaaacc tcttagaccg
    17941 cctgtaccaa attcgagcaa acttccgaaa cattcttcac gtgcattttt gttttcagca
    18001 gccagaaggt ttttagcctc tgcttgtgtc tcagcatcaa agcaagggtt ttcggcccag
    18061 aattgtactt tttcaattat gttcattgtt gatctggata tcagtgaaat tgtttttgct
    18121 taactactct gtttcaattc ttttaatatt ttttcattgt cagaacgaat tacagtttct
    18181 tttcttgtaa caggatgtgg gaatcggatt tctactgcat gcagtaattg tctatggagc
    18241 ggaaaatttt gtgaaagcaa ataatcgtca ccttcattaa gccagcgtat gaagccatca
    18301 tttggaagtc cataaagctt atcgcccaca acagaacagc ccatgtgtgc agcatgaact
    18361 ctgatttgat gagtccgacc actgaaggga cgcacttcaa caagtgaata atcacccatt
    18421 ttcccaattt ccctgaaata ggtatgggaa ggttttccta gagaatttac gctttgttta
    18481 atgcggatgt tgctgtttag gtcctgacca atcggcagcg aaatgaaaat ctccggaact
    18541 gttgtctttg aaatttgggg aaggtgtctg ctgaggattg cagaatatat tttgtgaatc
    18601 cggttttttc gaaaatgagc agccattgat tgggcaactt cgtgtctttt agcaaaaata
    18661 attaccccgc tggtttcacg gtcaagtcga tgcagagtgt aaagtttttt caggccaaac
    18721 tgagacttga tcaaactgac cagtgtattt ttgtaatact tacctgaagg acttgtcggc
    18781 aaatttcccg atttgcagac tgcaattaat gcatcatctt cgaaaataat ttcaaagtcc
    18841 agatcaacgt ccggttcaag aaaatctgga cgcaaataaa caattttttg attgtttcgc
    18901 agcttaaaat ttgtgcgccc caatttgccg tcgacccaaa tgcgtcgatc attgatattc
    18961 tcacgccact cctgatcatc caaatagcga aaacgccgga gtaaaaattc caacagcgtt
    19021 tcctcccgtc cttccacacg catataatgg aaagtcagtt cagtgtgctc ggatgtcatc
    19081 cggattgaca tatcaggcta agaaaggcta taaaatgttg aatcgttatt cactgagcaa
    19141 aagttccttt ccttcccagg aacttatttc ataaaacctg ccattcactt gctggttata
    19201 gattcccatt tcatcacgaa gttttgaatc ttctgcttca gaaccgcctg atgctaccag
    19261 tgaaataccc atccgcatgc tcaaaatata attttcattt gatgctatat tattccttag
    19321 gttcaatgta taaactctgc tgtcaaataa cgacacaaac aatgttgttt ctattaaatc
    19381 tgtaccttct gcttgctggg aaacagaatc atttatgtat aatttggaaa aagaaagctt
    19441 ccccagcctg ttgaggagag ttgttatttt atctgagtcc ggtacatctg atttttcaga
    19501 ggaggatttc cagtctgttt cagcactgtc acggttaatg gaaaatgaac ctccttcttc
    19561 gttcttaagc acaaggcttt ggactagatt tgactctaat gcaagtaaat cctttttcag
    19621 ccaatcattt gcttctgaat ctatggacaa gccttccgga attaaaaaaa ctttattaga
    19681 acctttgtgc cttacatact gccctggacc actagtacga ttctttccaa gaagaaagct
    19741 taatattgaa gttccgtctc cccgcaaaag gcttacagaa aatccatgtc gatcttcgct
    19801 ccaatcttcc ttgttttccg gaggagttaa tagttgaaaa cgttcatgat gatccggatt
    19861 atttgttacc agatctccca gacgtatttg agatagattg agcagcaaat cctgaatggg
    19921 aattggatct gcctcataat tcaaagactg ctctttccaa cctccgcctt ttaactgcat
    19981 taagtttaca gaagagccct gtgactcgat tttaatctgg ttcagggctt tggtgtcaag
    20041 ttgttctgga aagagtctat tgtcttttga tatttgtggt tttgtaaagt agttgtcatc
    20101 ttgataatag attaatccac ctccaatgat gaacaaacta aaagtagtga ataatagagg
    20161 tttaaacata attaaagcaa ttcagattat aatttcaaaa aataaattta tatccccatt
    20221 atctattatt ttacaacagg cttcaagact agaggtgtct ttttaatctt tcttaatcct
    20281 atttttggaa ttgggattac tttttttcaa atcatataaa cttgagattt ttcaagaagt
    20341 ttcgtaaatt taatgtttta tttactttga tcacaatgaa gtttttaagt gaatatttaa
    20401 ctcaggaagc acataaattt ttcaaacaaa cataagagat atgaaagaca atcagtttgg
    20461 ttgccggata aattttgaaa gtagcgaagg caaaactgct ctgtacagtt tagaaaagtt
    20521 gcaaaaagat ggaattggta cgatagacag actcccattt tccatacgga tattgttgga
    20581 acaggcactc cgtaacgttg atgattttca ggtttgtgct gaagacgtga cagcgctggc
    20641 caattggaat gctgcagaaa aatccaatca ggaaattcct tataaaccca cccgggtgat
    20701 tttacaggat tttacaggag tacctgcagt tgttgatttg gctgcactgc gttcagcgat
    20761 ggttgcaatg aatggcgatc cgagtgtaat taatccacgt ataccagtgg atttggtgat
    20821 tgaccattca atacaagttg atgcttttgg aagttcggga gcactcaaaa aaaatatgga
    20881 aattgagttc gtacgaaacc gtgaacgcta cgagtttctg aaatggggaa agcaggcttt
    20941 cgacaatttt aatgtctatc ctccaggtat cggaattgtg catcaagtga accttgaaac
    21001 ggtggcaact gttgttcaat cccgggatgg tgtttgtttt ccagacacat tggtgggcac
    21061 agattcacat accacaatga tcaatggtct tggagtaatg ggctggggag taggtggaat
    21121 tgaggcagaa tcggtgatgt taggtcaacc aatcaacatg ctcattccgg aagtgattgg
    21181 ttttcgtctt cttggagaac tgcaacagag agcaacggcg actgatttgg ttttacgggt
    21241 agttgaaatg ctgcgggaaa aaggtgtagt ggaaaaattt gttgaatttt ttggacctgg
    21301 tttgaataat ttgaaattag ccgaccgtgc aactctggca aatatggctc cggagtatgg
    21361 tgctaccatg ggttttttcc cagtagatga tgaaacttta agatacttgc gtgataccgg
    21421 acgaccggac ttgctggttc aactcgtaga acgctattgc aaagaacaag gtctcttccg
    21481 gacgagtgat actccagttc cggaattttc ggatactttg gagctggatc tttccactgt
    21541 tgaacctgcg cttgcaggtc ctaaacgtcc acaggatcgt gtcaatctta cgcaaatgca
    21601 gcaaaactgg caaaaaactt tgcgtgtatc caggaaagag cgtggatttg aattgagtga
    21661 tgagcaaata gaaacctcgg ttcagatgca gggtattcct tccggaaaac taaaacatgg
    21721 ttcggtggtc attgcggcaa ttacgtcctg tacaaacacg agcaatcctt ccgttatgct
    21781 tgcagcagga ctgcttgcaa aaaaagcagt tgaaaaaggc ttgaatcgcc aaccttgggt
    21841 taaaacatct cttgggccgg gttcacgtgt ggtgacagat tatctggaag cagccggatt
    21901 gaatgaaccg cttgatgcac ttggttttca tactgttggc tatggctgta ctacctgtat
    21961 tggtaactca ggtcctcttc cagaaagtat tgttacagca ataaatcaag gcgatttggt
    22021 agttgcctca gttttgtctg gaaatcgaaa ttttgaagga cggataagtc agcatatcaa
    22081 ggctaattat ttggccagtc ctccgctggt tgtagcttat gccattgctg gaacggtgaa
    22141 tcatgattta ataaatgagc ctcttggcaa cggaaaagat ggtcagccgg tttttctaaa
    22201 agacatttgg cccgctcagg aagaaatagg tgaagtagaa aaactgatca ccagcgaaat
    22261 gtatgagaaa gaatacaaag gggcaggtga aagctctccc ctgtggaatg ctattgaagc
    22321 gcgaacaggt gaagtttatg aatgggatga ttcatcgacc tatatccaaa atccaccatt
    22381 ttttcaaaaa atgggtcttg atgttaatgc tttgcaggat attactggtg caagagtttt
    22441 agcaaaatta ggagattcga ttacaacaga tcatatttcc cctgccggag ctttttcctc
    22501 ggaaactcca gcagggaaat atttactgga acttggaacg gaaatcaaag atttcaattc
    22561 ttatggatcc cgtcgcggga atgaccgtgt catgacgcgg ggaacttttg ggaatgtccg
    22621 tttgcgaaat gagttggttc ccggaacaga gggtggtttt accaaactaa tcccttccgg
    22681 agaacttaca acaatttacg aagcagcaca aaaatataaa gatagtggaa ctccgctagt
    22741 tgttttagca ggtgccgaat atggaactgg ctcctcgcgt gattgggctg caaaaggtac
    22801 attgctgctt ggtgttaagg ctgtagttgc aacgagtttt gagcgtattc accgctccaa
    22861 cctggtgggg atgggtgtct tgcctcttca attcaagaac ggtgaaactc ataagtcact
    22921 tggcttgagc ggagaggaag agtatgcggt gttgggttta actgataatt tgaaggcggg
    22981 ccaggatgta atcttacgtg cagatgatcg cgaaattaca gtcacttgtc gtttagatac
    23041 gctggttgaa ttagaatttt acaaaaacgg tggaatttta cacacagttc tgagaaattt
    23101 catgcgagaa agtcttaggg aatagtgttg aaagattaac taattattag tgtcgaaatg
    23161 caggatgtac ttttactaaa tcaagacggg aacccgttga cactgtggcc tttgagtaca
    23221 ctctcctggc aacaggctat caaggctctt tacctagata aggtgactgt tcttcgttct
    23281 tatgatgatt ggatatgtca ctcccagcat ttggccttac ccgtgccaag tgtggttatg
    23341 atgtcacgtt atcatcaaca gcaaggtacg gtcaatttca ctcgccgcaa cattttcctg
    23401 cgggaccgtt ttcattgcca atattgtctg catcaatttt cagcagatca tcttactatt
    23461 gatcacgtaa ttccaaaaag ccgaggcgga ggaatgcgtt ggaaaaatgt ggtagcagcc
    23521 tgtcacaaat gcaatttaag taaaggcgca aatattgtcc atccctctca gactccgttt
    23581 gaaccaaact attggcaaat ggtcaaaatc gccaaaactc tccatatcaa aattcctcat
    23641 tccagttggc aggaatattt gcagtggcca attcatctgg tgaagattga tcaggcaaag
    23701 gctgcttaac cgttctttcg ttcaaattct ttcatgaact gagccagtgc atcaactcct
    23761 tcaagaggca ttgcattgta aatggatgct cttactccac caactgaagc atgaccttta
    23821 agtgcgaaca atccttcttt gtcagtttca gttaaaaatt tctggagtag tgtttcttgc
    23881 ggcaaatgaa atgttacgtt agttattgag cggtgttttg gatgtgctac agaactgtat
    23941 aattctgaat tatccagaat ctcatacagc aaactggatt ttttttcagc cagtttttcc
    24001 atttccggaa cccctccttg ttcttttacc cattccaaaa ccaaacgcat tacgtaaact
    24061 gcaaatacat tgatggtatt tggtatggag ttgtgttcat caaaaagagc ataatcaagc
    24121 agttttggtg tttcaggcag tgcatgaccg agtagatctt cacggaccac cactaaacct
    24181 gttccggaag ttccaagatt tttttgtaaa cccgcataaa ctattccaaa ttgtgaataa
    24241 tccatttccc tggataaaat atccgaagtt gcatcggcca cgagtggtac attccctgtg
    24301 tcaggaaatt ggtgccagcg agttccgtag agtgtgttgt ttgtcgtcag gtgcgcgtag
    24361 ctggaatcct ggtccagtga actggtgtca tattgcggaa tttgccgata ctcaaactct
    24421 ttaccatcca tcagtatctc ggtattccca tatttcctgg cctcattttc tgcaagtgta
    24481 ccccaccttc cagtgataaa atacgaccct ttgtgagact tcattgacat caaatttaat
    24541 ggaactgcag aaaattgcat ttgagcacca ccatgcatga aaagcacttt gtaattattt
    24601 ggcagaccag ttaactcacg aaaaagagat ttggtagttt ctataatgtc tttaaaaact
    24661 ggaagtctgt ggctgatttc aacaattgag gctccaagtc catgaaagtc caagaattct
    24721 ttttgaactg tctccattac aggaatcggc agcgtagccg gtcctgcact aaaattataa
    24781 acgcggttag tcatttaatg tcctggttaa tttgttttta taaacagtta ttaaggttct
    24841 ctgaaaaagt attgttaaag cagtcgcgga cttgatctgt gctacggatt tcagactagg
    24901 ttggggtctg gatattagat aatgatccgg
//
LOCUS       DTVD01000160            7420 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2902_length_7420_cov_317.679022, whole genome
            shotgun sequence.
ACCESSION   DTVD01000160 DTVD01000000
VERSION     DTVD01000160.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_7420_cov_317.679022"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(531..1589)
                     /locus_tag="EYO68_05620"
     CDS             complement(531..1589)
                     /locus_tag="EYO68_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HIC07319.1"
                     /translation="MKQNALGSSGIKVSCLGLGTMTFGEQNSEEEAFAQMDCALAAGV
                     NLFDTAEMYPVPPKENTFTISEQIVGNWIKLRKCRERIVLATKVVGPTVESRSMGSYI
                     RDGLNHLTKKNIRAALEGSLSRLKTETIDLYQIHWPDRNVNIFGKRGFVPSENSETDN
                     EGIPETLETLNKLREEGKVQAFGVSNETPWGVMRYLSLSEKNRWEKVASIQNPYSLLN
                     RTFESGLAEITYRESVGLLAYSPLAFGMLTGKYLDGLKPARARLTLFSRFQRYQTKNA
                     IKATRDYVKLAWEHALDPAQMALAFVRTRPFMASVLLGATSVKQLDTNLASVELELSA
                     EVLEGIEEIHGRIPNPCP"
     gene            complement(1619..2446)
                     /locus_tag="EYO68_05625"
     CDS             complement(1619..2446)
                     /locus_tag="EYO68_05625"
                     /inference="COORDINATES: protein motif:HMM:PF13174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07320.1"
                     /translation="MFSIDLKTTSTIISTFKKSRKRTVKRLIMSITAPGHIVSFLIIL
                     IFFVGGCSSSGANKTTPLNNTEEMAALKKTLAQQEELLDRLQALTADQLLRSNEIEQS
                     MPPRDLLESLQKGFIELQKKTLILEKQLSELKKGLETSGSKEKPSKSSANNFSINQEK
                     IILGLVSLQSGNPDQAVEHLQDILKAKKATKLKGDILMAIGNGFLAQGHSKQAASHYG
                     LFLREYPKSRHTPQALYYLGQAMKDLGEVEKQKILWKELIKNYPQSSLAKRAKKRLR"
     gene            complement(2471..3754)
                     /locus_tag="EYO68_05630"
     CDS             complement(2471..3754)
                     /locus_tag="EYO68_05630"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07321.1"
                     /translation="MKSTNYVYQLKLRMRAGLVFLLFGLFILTCGGSVSAIDYFEITN
                     PKFIPVKVGLSKESSPEVVFLQNVFKTNLEKVLYFQFITESKEEFDQSEEVIFHLDLE
                     LKSKPFLLKIMLWSQGEKTPVSKELYPVGTNQDLRKLGLKLADWFVENTLGFEGVSTS
                     RISYTAQKTNRRKNIFLSSFDGTVLKRFSYNLGSNNLSSWSFDGNNILYTTLTRSSAQ
                     LAIQPAKRLRAKILSFPSGFQPLSASWNLDGESILLTLMKQGNADIYNYHLENAQLDK
                     IFAWKSLETSPQMSPDGRKIAFVSDSARPRRPQIYIHNLNTLKTERVTFKGNFNSSPK
                     WSPNGTQLIYERQEKGYFQLYIYTLSTRRHLQLTFGRFNSEKPDWAPNGKQIVFSAKK
                     KNVPKLYYISSFGGRTIRVTKTPSNIAETNPVWSK"
     gene            complement(3765..4523)
                     /locus_tag="EYO68_05635"
     CDS             complement(3765..4523)
                     /locus_tag="EYO68_05635"
                     /inference="COORDINATES: protein motif:HMM:PF13103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07322.1"
                     /translation="MTLKWRFSGGILFSLLLHFFLLSALLYWYPGMIGRSDQPWQEWS
                     QFLAEKILPSGTVSKTTPETSAKMTTATLIFPDIVFYRQQKKPLIITPSKKKKSKPKL
                     SFNNILSQIEKFPERLKVSKDSKKPFSSQVVSLLTLLKPVSLQNQKAKIGTTNLNAVE
                     LLNYRKELEAFLSEQWKVPINLVGSTNTVVVKFEINKDGRILKWYQAETGNTLLYKSV
                     KNLLKNLQFLPALPKSYPEDSYKFGIRFSPANFK"
     gene            complement(4536..5354)
                     /locus_tag="EYO68_05640"
     CDS             complement(4536..5354)
                     /locus_tag="EYO68_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIC07323.1"
                     /translation="MNENKIKLRKVGIFSQQLVHPVTELLQEWFVPHGIEITTLADGE
                     KSETGLDLIVSFGGDGTVLAALSLFPECPVLAVNFGNVGFLTAGDREDLTDMLQSVLD
                     GNYIISERSVLECLHPLGTDYAVNEIVVRGATRLIAVELSINGKHIRRVRGDGVIVGT
                     ATGSTAYLLAAGSPIVIPELRCMIIAGLNEYDFRSRHLVVTGESKIRLVISEQTHEKE
                     IYLSADGKEKVPLKIGDEVLIQESARQAKLVFMEKNYFFHNLSSRLSWFHSGEK"
     gene            5579..7384
                     /locus_tag="EYO68_05645"
     CDS             5579..7384
                     /locus_tag="EYO68_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIC07324.1"
                     /translation="MNTFLRNIGIWLVIGLVMLMLFNLVGPRESNEREISFSEFISQI
                     ESGSVLEVIIRGSQIHGVSDTNGSFQTQVPNYPALFKILDSHQVRVKVEPTDETNLFM
                     AILSSWLPMILIIGIWLFFMRQVQGGGNRAMGFGKIRSRITNTEDNPIKFEDVQGIDE
                     SREELEEIVDFLKDPGKFERLGGEIPRGVLLMGEPGTGKTLLAKAIAGEAAVPFFSIS
                     GSDFVEMFVGVGASRVRDMFAQGKKHAPCIIFIDEIDAVGRSRGAGLGGGNDEREQTL
                     NQLLVEMDGFEVNEGIIVVAATNRPDVLDHALLRPGRFDRHVVVPSPDIKGRENILKT
                     HSKKIELDKKVSLNKIARGTPGFTGADLANLVNEAALWAARQDKETVDNDDFEYARDR
                     VMMGAERRSLLISDEEKETTAYHEVGHALVAASIEEVDPVHKVSIIPRGRALGVTMLL
                     PEEDRHSHNRRSLLGQITMTMGGRAAEHLVFKRFTTGASDDLKRATELARKMVCQWGM
                     SEKLGPLTYTEDAGHVFLGRDLQQHTEFSNESMRMIDEEVLEILNSSYERAKSILKTY
                     RKALESLALTLLEKETIDGENVLQVMEEFAPKTKK"
BASE COUNT         2116 a         1489 c         1579 g         2236 t
ORIGIN      
        1 ttttggacat ccgccacaca ggccggacct attgacggtt gggggttatt tttggatgta
       61 ggtcaattaa aaccggttaa tgatgacagt ggaagagagt accagggttc caaaataatc
      121 ggagatcaga ttgactatca gtttgctttg ggggattctt tttctttctc acttttcgct
      181 tcagaaaaca tgaacaaagg agtattgccg gcaaacacaa aatatgggta ttacaaagcc
      241 ggcatcctag gtgcagaatt tcgggcatgg atggggccat tatttatagg tgtacatgga
      301 gggcaatatt ttctgacatg gattgaatct ttgagttctt atacaggact taaatggtcc
      361 ggaggcagcg gctggggact tggtctcgaa ggaaaatcag gatggtctct tggttggtac
      421 aaggaaaaaa gtgaaaaaat tgatttcgat gattttcccg atcaacgaat tgaaggagac
      481 cgattcatac tcggctaccg ctggcgttaa tgtagttcct cttaattctg tcagggacat
      541 ggatttggaa ttcggccgtg tatttcttca ataccttcca gcacttctgc tgaaagttct
      601 aattcgacgc tggcgaggtt tgtatccaat tgtttgacag atgtagcccc aagcagtaca
      661 ctcgccataa acggccttgt ccgcacaaaa gccagtgcca tttgcgcggg atccaaagca
      721 tgctcccatg caagtttgac ataatctcgt gtagccttta tcgcattttt tgtctggtaa
      781 cgctgaaaac gggaaaacag agttaaacgc gcacgtgccg gtttgagtcc gtcgagatat
      841 tttccagtca gcattccgaa agcaagcgga gaataagcca gcagcccaac tgattcccgg
      901 tacgtaattt cagcaagtcc ggattcaaaa gtgcggttga gcagactata cggattctga
      961 atactggcaa ctttttccca tctgtttttt tcagacagac tgaggtaacg catcacgccc
     1021 catggcgttt cattggaaac tccgaaagcc tgcactttcc cttcctccct gagtttattc
     1081 agcgtttcca gggtttccgg aattccttca ttgtctgttt ctgaattttc cgaaggcaca
     1141 aatccccgtt ttccaaaaat attaacattc cggtccggcc agtgaatctg atagagatca
     1201 attgtttcag tttttaaacg actcaaactg ccttcaagag ctgctctgat attttttttt
     1261 gtgaggtgat ttagtccatc ccgaatgtag ctccccattg aacgagattc tacagtgggt
     1321 ccaaccactt ttgtagctaa aacaatgcgc tccctgcatt ttcggagttt aatccaattt
     1381 ccaacaatct gttccgaaat agtgaaagta ttttctttag gtggtaccgg atacatttcg
     1441 gctgtatcaa acaggtttac acctgctgca agtgcacaat ccatttgagc aaatgcttcc
     1501 tcttctgaat tctgttcacc aaaagtcatt gtaccaagtc cgagacaact gacttttatc
     1561 ccggaacttc ctagagcatt ttgtttcatt ttaaagatta atttgcagtt ccagtagatt
     1621 atcgcaaccg ttttttcgca cgtttggcaa gagatgattg aggataattt tttatcaact
     1681 ccttccacaa aatcttttgt ttttctactt caccaagatc tttcattgct tgtcccaaat
     1741 aatacaaagc ctggggagta tgccgacttt ttggatattc gcgaagaaaa agtccgtaat
     1801 gagatgctgc ctgctttgaa tgaccctgtg ctaaaaatcc atttccgatt gccatcagga
     1861 tgtcaccttt taattttgta gccttttttg ctttcaagat atcctgcaga tgctccaccg
     1921 cctgatccgg attacctgac tgtaatgaaa ctagtcccag aataattttc tcttgattga
     1981 tggaaaaatt atttgcagaa gatttagagg gtttttcctt acttccggat gtttccaaac
     2041 cttttttcag ctcagatagc tgtttttcaa gtatcaatgt ttttttctgc agttcaataa
     2101 atcctttttg taaagattcg agcaaatccc gcggcggcat agattgttca atttcgttgc
     2161 tgcgcagcag ctgatctgct gtcagtgcct gcagtcgatc caacaattct tcctgctgtg
     2221 ccagggtttt ctttaaagca gccatttctt ccgtattatt tagcggagtt gttttattag
     2281 ctcccgagga agaacaaccc ccaacaaaaa atatgagaat gatcaaaaat gaaacaatat
     2341 gtcccggtgc tgttatggac ataataagtc gttttactgt ccgcttccgt gacttcttga
     2401 atgttgaaat aattgttgaa gttgttttca aatcaatact gaacatttat taatcgtgcg
     2461 gtaaattagg ctatttactc cagacaggat ttgtctcggc gatgttggaa ggagtcttag
     2521 tgacacgaat ggtgcggcct ccaaaagatg aaatatagta aagttttgga acatttttct
     2581 tttttgcgga gaaaacaatt tgtttcccgt ttggcgccca gtcaggtttt tcagaattaa
     2641 aacgtccaaa tgtcagctga agatgacggc gtgtagataa ggtgtatata taaagttgaa
     2701 aataaccttt ttcctgcctt tcataaatca gttgtgttcc atttggtgac cattttggag
     2761 aactgttgaa attaccttta aaagtcacac gttctgtttt aagagtattt aaattatgga
     2821 tataaatttg tggacggcgt ggacgtgcac tgtctgaaac aaatgctatc ttcctgccat
     2881 ccggagacat ttgcggagag gtttccaggc ttttccaggc aaaaatctta tccagctggg
     2941 cattttccaa atgatagttg tagatgtcag cattaccctg cttcatcaac gtcagcaaaa
     3001 ttgattcgcc atccagattc cagcttgcgc tcagcggttg aaatccggaa ggaaatgaaa
     3061 gaattttggc acgaagacgt tttgcaggct gaatggccag ttgtgcgctg gaacgtgtga
     3121 gtgttgtata aaggatgtta ttaccgtcga aagaccaact tgacagatta ttggaaccaa
     3181 gattgtatga aaatcgcttt agcacagttc catcaaaact actgagaaat atgttttttc
     3241 gccgattcgt tttttgtgca gtgtaggaaa ttctgctggt ggatacccct tcaaatccta
     3301 gcgtgttttc tacaaaccag tcagccagtt ttagcccgag ctttctcaaa tcctgatttg
     3361 ttccgaccgg atacaattct ttggaaacag gagttttttc tccttgggac cagagcataa
     3421 tttttaatag aaacggctta ctttttagct ccagatcaag gtgaaaaata acctcttcgg
     3481 actgatcaaa ctcttctttt gattcagtaa taaactgaaa ataaagtact ttttctaaat
     3541 tagttttgaa aacattctgc aaaaatacga cttcaggact gctctctttt gaaagtccga
     3601 ctttgacagg aatgaacttt ggatttgtga tttcgaaata atcaatggcg gaaacactgc
     3661 ccccgcaagt aagtatgaac aatccaaaca gcagaaaaac taaaccagcc cgcatgcgta
     3721 attttagctg ataaacatag tttgtggact tcactggaat ctcattattt gaagttagcc
     3781 ggactgaagc ggataccaaa tttgtatgaa tcttctggat aggactttgg caaggctggg
     3841 agaaattgca agtttttcag taaatttttc acagatttgt acagtaaagt attgccggtt
     3901 tctgcctggt accattttaa aattcggccg tctttgttaa tttcaaactt aaccacaacg
     3961 gtatttgtac ttccaaccaa attgattgga actttccact gttcagacaa aaacgcttca
     4021 agttccttac ggtaattcaa caattctacc gcatttaaat tggtagttcc aattttcgct
     4081 ttctggtttt gcagactaac cggtttcaaa agtgttaata gtgaaaccac ttggctggaa
     4141 aacggttttt ttgaatcttt ggatactttc agacgttcag gaaatttttc tatttggctg
     4201 agtatgttat taaaagacag ttttggttta gattttttct ttttgctggg agtaataatc
     4261 aggggctttt tttgttgcct gtaaaaaaca atatcaggaa aaatcaaagt agcagttgtc
     4321 atttttgctg aagtctccgg agttgttttg ctgactgtac cagagggcag tattttttca
     4381 gctaaaaact gggaccattc ctgccatggt tggtcactcc tgccgatcat tccgggatac
     4441 cagtatagca aggcagataa caaaaaaaaa tgtaataaca atgaaaacag gattccacct
     4501 gaaaaacgcc atttcaaagt cataaaccga tccaactatt tttcaccact gtggaaccag
     4561 gacagtcgtg aagaaagatt gtgaaaaaaa taattctttt ccatgaaaac taattttgcc
     4621 tgtcttgccg actcctgaat gagaacttca tctccaattt taagcggcac cttttctttt
     4681 ccatccgcag aaagataaat ttctttttca tgagtctgtt ctgatattac aagcctgatt
     4741 ttggactctc cggtgacaac cagatgtctg gacctgaaat catactcgtt caagcctgcg
     4801 atgatcatac aacgtagttc cggaattaca attggagatc cggcggcaag taaatatgca
     4861 gtacttccgg ttgcagttcc cacgataact ccgtctccgc gaacacgtcg tatatgcttc
     4921 ccgttgatgc ttaactcaac tgcgattagt cgagttgcac cacggactac aatttcattc
     4981 actgcatagt cagttccaag aggatgaagg cactctagta ctgaacgttc actgattata
     5041 taattcccgt caagtacact ttgcagcata tcagtcaaat cttcacggtc tcctgcagtc
     5101 aaaaaaccga cattaccgaa attgactgcc aaaaccggac actccggaaa aagacttaat
     5161 gctgcaagta cggtcccatc tccgccaaat gagacaatta aatccaaacc ggtctcactt
     5221 ttttccccat cagcgagagt ggtaatttca ataccgtgag gaacaaacca ctcttgcaac
     5281 aactccgtta ccggatgtac cagttgctga gaaaaaatac cgactttgcg cagcttgatt
     5341 ttattttcgt tcaccactct ttatttgaaa aaaattacca tgtctttaca gtatcttcgg
     5401 gcactttcgg cttccttgtc aagtaaaaaa ccccggccgg ttgtctcctg cataaattga
     5461 ttacaaaaaa ttattaaaat aaatgcttaa aatccggttt ttagtctcag acttgcgttt
     5521 cctgtgtata tatgtgatga tctaccagtt aaaacaataa catttatttc agacaactat
     5581 gaatacattc ttacgtaata tcggcatctg gcttgtgatc ggccttgtaa tgctgatgct
     5641 ctttaatctg gttggaccaa gagaatcaaa cgagagggaa atttcttttt cagaatttat
     5701 ttctcaaatc gaatccggtt ctgtgctgga agttataatc cgtggaagtc aaattcatgg
     5761 tgtcagcgat acaaatggtt cttttcaaac tcaagttccg aattaccctg cactgttcaa
     5821 aatattggac agccatcaag tacgcgttaa agttgagcca accgatgaaa ctaatttatt
     5881 tatggccatc ctgagcagtt ggcttcctat gatcctgatc attggtatat ggctcttctt
     5941 catgcgtcag gtccagggtg gaggaaaccg tgccatgggt tttggaaaaa tccgctcacg
     6001 aataaccaac acggaagaca atccgataaa atttgaagat gttcagggaa ttgatgaatc
     6061 acgggaagaa ttggaggaaa tcgtagattt tctgaaagac cccggaaaat ttgaaagact
     6121 tggaggagaa atcccaagag gtgttttatt aatgggagaa cctggaaccg gaaaaaccct
     6181 gctggccaaa gcaattgcag gtgaggctgc agtccctttt ttcagtatca gcggttccga
     6241 ttttgtggaa atgttcgttg gtgtcggtgc cagccgtgtc agggacatgt ttgcgcaagg
     6301 taaaaaacac gcaccgtgta tcattttcat tgatgaaatc gatgcagtcg gacgcagccg
     6361 tggtgccggt cttggagggg gaaacgatga acgtgaacag actctgaacc agttactggt
     6421 tgagatggat ggttttgaag taaatgaagg cattattgtt gtagctgcaa ctaatcgacc
     6481 ggatgtgctg gatcatgctt tgctgcgtcc agggcgtttt gaccggcatg tagttgtacc
     6541 ttctccggat atcaaaggtc gtgaaaatat tttgaaaacg cacagtaaaa agattgaact
     6601 ggataaaaaa gtaagtttga acaaaatagc acgtggaact cctggattta ccggtgcaga
     6661 cctggctaat cttgtcaatg aagcagcact ttgggctgcg cgtcaggata aagaaactgt
     6721 ggataatgat gattttgaat atgcaagaga tagagtgatg atgggcgcag agcggcgcag
     6781 tctcttaatt agtgatgaag aaaaagagac tacagcctat catgaggttg gacatgctct
     6841 tgttgcagcc agtattgaag aagttgaccc cgtgcataag gtgagtataa ttccgcgagg
     6901 acgggcttta ggagttacaa tgctcctgcc ggaagaagat cgacacagtc ataacaggcg
     6961 ttctttgctg gggcaaataa ccatgacgat gggaggacgt gcggcagaac atttagtatt
     7021 taaacgcttc actacaggtg ccagcgatga cctgaaaaga gctacagaat tagcccgaaa
     7081 aatggtttgc cagtggggaa tgagtgagaa actgggacca ctgacttata ctgaagatgc
     7141 ggggcatgtt tttctgggac gcgatttaca acagcatact gaattcagca atgagtccat
     7201 gcgtatgatc gatgaagaag tgcttgaaat cctaaattca tcttatgagc gggcaaaatc
     7261 aattttaaaa acttaccgta aagcacttga atctcttgcc ctgacattgc tggaaaaaga
     7321 aactattgat ggagaaaatg tgctgcaggt tatggaggag tttgcaccaa aaactaaaaa
     7381 atagtgtctg aaaatttttt tgattttcaa ataactttga
//
LOCUS       DTVD01000161            1825 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_29211_length_1825_cov_177.888701, whole genome
            shotgun sequence.
ACCESSION   DTVD01000161 DTVD01000000
VERSION     DTVD01000161.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29211_length_1825_cov_177.888701"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="EYO68_05650"
     CDS             <1..592
                     /locus_tag="EYO68_05650"
                     /inference="COORDINATES: protein motif:HMM:PF16576.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIC07325.1"
                     /translation="EQGSSTRSQLDVLQLQYLKGEIALKNSILGLERAILNVDNTILS
                     LERSREDIKGSELSVENALIAVERSSHDVELSEDALEDTLIKAKIGGIITAKSFQEGE
                     VITAGAELFQIIDIKQVEIKIQLGEGDLPMISEGQAVVFTTPGYRDIEFSGIIERISW
                     TADPETGRFPLYVKASNPGLKLRAGMSVKVYLLWEK"
     gene            complement(605..1666)
                     /locus_tag="EYO68_05655"
     CDS             complement(605..1666)
                     /locus_tag="EYO68_05655"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIC07326.1"
                     /translation="MLNSNQQSVISNNHIGILLINLGTPDTPHTRDARAFLRGMLSSK
                     RFLDINPILRWLLLNLVILPFRPRKTANALKKIWLEDGSPLLVHSQKLKNALVEKFTE
                     DKIPVELGMLLGKNNLKSAVDRLRRQECDRIIVLPMFPQYSATTHGSAIEALYNEILH
                     DWDIPVIQIIPPFYSDPRFIEAWAKVGSPYLVNEPDHVLFSYHSLPVKHIQKSDCSGT
                     WCKNDNACCLELVNENRNCYSAQCFQTAKLLAGRLNLETEKWSVSFQSRLGSAEWIKP
                     YTEQHLTKLAEQGVKKVVVFCPSFVVDNLETLEEIEIRAAEHFRKSGGEKLTLVPSLN
                     YHPEWVAALTSIIRQQLAG"
BASE COUNT          541 a          374 c          372 g          538 t
ORIGIN      
        1 cgaacaaggt tcgtctactc gttcgcaatt ggacgtactg cagctgcagt acctcaaagg
       61 agaaattgca ttaaaaaatt ctatacttgg attagagcgt gccatactaa atgtggacaa
      121 taccattctg agtcttgaac gttccagaga agatataaag ggttccgaac ttagtgtgga
      181 gaatgcgctt atagcagtag agcgttctag ccatgacgta gaacttagtg aagatgctct
      241 tgaagacact ctgatcaaag caaaaattgg cggcataatt actgcgaaat cattccagga
      301 aggagaagtg ataaccgcag gtgcagagct ttttcaaatc atcgacatca aacaggttga
      361 aatcaagatt caattaggag agggagattt gccgatgatt agtgaggggc aggctgttgt
      421 ttttaccaca ccaggttatc gtgacataga attttcagga atcattgagc gcatctcctg
      481 gacagcagat ccggaaacag ggcgtttccc tctgtatgtc aaagcttcaa atccaggcct
      541 caagttaagg gccggtatga gtgtaaaagt ttatttgctc tgggaaaaat aattttgctg
      601 aattttatcc ggccaactgt tgccggataa ttgaagtcag tgccgcaacc cattccggat
      661 gatagttaag tgaaggaaca agtgttagtt tttctccacc acttttccgg aaatgttctg
      721 ctgcacgtat ctctatttct tccaacgttt ccagattatc tacaacgaat gaaggacaaa
      781 aaacaacaac tttcttcaca ccttgttccg caagttttgt gagatgttgt tcagtgtatg
      841 gtttgatcca ctcagcactg cctaaacggg actgaaaact taccgaccat ttttctgttt
      901 ccaaatttaa gcgtccggcc agcaattttg cagtttggaa acactgcgca ctatagcagt
      961 tgcggttctc attaactaat tccaaacaac atgcattatc attcttgcac caggttccgc
     1021 tgcagtcgct tttctgaatg tgcttaactg gaagactgtg ataactgaac agtacatgat
     1081 ctggttcatt tacaaggtaa ggtgacccta cttttgccca agcctctata aaacgaggat
     1141 ctgaataaaa tggtggaatt atctgaatga ctggtatatc ccagtcgtgc aaaatttcat
     1201 tgtaaagagc ctcaattgca gatccatgag tagtggccga gtattgggga aacatgggca
     1261 aaacgattat ccggtcacat tcctgcctgc gcaatcgatc cactgcactt ttcaagttat
     1321 tttttcctag tagcattccc aactctacag gaattttatc ttcagtaaat ttctcaacca
     1381 gggcgttttt taacttctga ctatgaacca aaagtggaga tccatcttcc aaccaaattt
     1441 tttttaaggc attagcggtt ttcctgggcc gaaaaggtaa aattacaaga tttaacaaca
     1501 accagcgcaa gatcggattt atatccaaaa atctcttgga ggaaagcata ccccttaaaa
     1561 aagcacgggc atcccgcgta tgaggagtat ccggtgttcc gagatttatc agtaaaatac
     1621 cgatatgatt attgctgatt actgattgct gattactgtt tagcatattg ttgttcctta
     1681 aagtgtcaaa agccaaaaga cctgccgaat gaatcacaaa gtactcattt gactaagtcg
     1741 taaatatttt ttcctgtctt tccgcagctt tttttatgtt gtataaaaag cgttttcttc
     1801 aatatagccc ttagtcaaat cacaa
//
LOCUS       DTVD01000162            1821 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_29312_length_1821_cov_134.464326, whole genome
            shotgun sequence.
ACCESSION   DTVD01000162 DTVD01000000
VERSION     DTVD01000162.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29312_length_1821_cov_134.464326"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            12..>1821
                     /locus_tag="EYO68_05660"
     CDS             12..>1821
                     /locus_tag="EYO68_05660"
                     /inference="COORDINATES: protein motif:HMM:PF00211.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIC07327.1"
                     /translation="MKDEKPTRKLATILAADCVGFSRLMDKNEELTLQNIKKCRNLID
                     PVITENGGRIFHTAGDSVIAEFNSVVDSVNAAIEFQKMLHERNENVPEDSRMVFRVGM
                     HLDDVIIEGDNVYGSGVNVAARLEGLCEPGCILLSRTVHEKISKRIQVAIDSLGNAQL
                     KNIEGDFEIYQISPILKNGIKPIDPTKLTTKSSPTESKIVTENKDKNAKPRLMILPFR
                     NVNKSEDNDFLVDGIVDDIITEFSMINFIEIMSRNTTFEYKDNPVDVREAAEKYKLDY
                     VVTGSVRSAGNRVRISAELGDPFTGDSIWSERYDKTMDDVFEIQDEIVRKVSSAVLGE
                     IEITSLKRAKRKPTEDMTSYEYFLQGVYHKRKNTKEDVNKAVEMFTKAIENDENNGRA
                     YAQRCCTWGGALGKNWLNEPDNELIPKILSTLEEAYELTDHDWDCHRLLCAVSLFFHA
                     NYDKAEEHGRKAYELNPNNHAVLTIYGQSLIFNGECERGIEILEKAFELDPMNQELVD
                     KIIWGYYAMADYEACIYHTNKIKKMGPNNWLLKIASLGALMRYEERDTEMNLFSDAHG
                     KDELKLQFDNLNFNNEEIIESTKNFIFNELESGNVVF"
BASE COUNT          644 a          288 c          402 g          487 t
ORIGIN      
        1 aatgcataat tatgaaagat gaaaaaccaa ccagaaaact ggcgacgatt ttagcggctg
       61 actgcgtggg attcagcagg ttgatggaca agaatgaaga gttgacccta caaaatataa
      121 aaaaatgccg taatctgatt gatcctgtca tcacagaaaa tggagggaga attttccata
      181 cggctggtga ttcagtaatt gcggagttca acagtgttgt agattctgta aatgcagcca
      241 tagaatttca gaaaatgctg catgaacgca atgaaaatgt gcccgaagat tccaggatgg
      301 tattccgggt ggggatgcac ttggatgacg tcatcattga aggcgataac gtctatggta
      361 gtggagtcaa tgtcgctgct agactagaag ggctttgtga gccaggttgt atactacttt
      421 ccaggacggt tcacgaaaaa atttctaaac gtattcaagt ggcaatcgac agtctcggca
      481 acgcccaact aaaaaatatt gaaggtgatt ttgaaatcta ccaaatttct ccaatattga
      541 agaatgggat taaaccaata gacccaacta aactgaccac caaatcctca cctacggaaa
      601 gtaaaattgt aacagaaaat aaggataaaa atgctaagcc tcgtttaatg atattaccat
      661 ttagaaacgt gaacaagagt gaggacaatg actttctggt ggatgggatt gttgatgaca
      721 ttattactga gttttccatg atcaatttta ttgaaataat gtcacgcaac actacctttg
      781 aatacaaaga taatcctgtt gacgtcagag aagctgcaga aaaatacaaa ttggattatg
      841 tcgtcacagg tagtgtcaga tcagctggca atcgagttcg catttcagct gaattggggg
      901 atccatttac aggagattcg atatggagcg aacggtacga caagactatg gacgatgttt
      961 ttgaaattca ggatgaaata gtaagaaagg tatcatctgc ggtcctgggt gaaattgaaa
     1021 ttactagttt gaaaagagca aaaagaaaac caacagagga tatgacctca tatgaatatt
     1081 tcctgcaggg tgtatatcat aaaagaaaaa atacgaaaga ggatgtaaac aaagccgtag
     1141 aaatgttcac aaaagctatt gaaaatgatg agaacaatgg aagggcatat gctcaaagat
     1201 gctgcacttg gggtggagct cttggaaaaa attggttaaa tgaacccgat aacgaattga
     1261 ttcctaaaat tctttcaaca cttgaagaag catacgaact gacagatcac gattgggatt
     1321 gccatcggtt actttgtgcc gtttcattgt tctttcatgc aaattatgac aaggcagaag
     1381 aacatggacg taaagcttat gaactgaatc caaataatca tgcagtttta actatttatg
     1441 gacaatcgct tatctttaat ggtgaatgtg aaagaggtat tgaaattctc gaaaaagcat
     1501 ttgagttaga tcccatgaat caggaactgg tcgacaaaat aatttgggga tattatgcaa
     1561 tggctgatta tgaagcgtgt atctaccaca ctaacaaaat taaaaaaatg ggaccaaata
     1621 attggcttct gaagattgcc agtttgggtg ccttgatgcg atatgaagaa agagacacgg
     1681 aaatgaatct tttctctgat gcgcatggga aagatgaatt gaaattgcag tttgacaatt
     1741 taaatttcaa taatgaggaa ataattgagt caacaaaaaa tttcattttt aacgaacttg
     1801 aatccggaaa cgttgtcttc a
//
LOCUS       DTVD01000163            1817 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_29441_length_1817_cov_335.825766, whole genome
            shotgun sequence.
ACCESSION   DTVD01000163 DTVD01000000
VERSION     DTVD01000163.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29441_length_1817_cov_335.825766"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            159..1415
                     /locus_tag="EYO68_05665"
     CDS             159..1415
                     /locus_tag="EYO68_05665"
                     /inference="COORDINATES: protein motif:HMM:PF01943.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIC07328.1"
                     /translation="MPVSADFNQVSNLRNNLSWNFVGQVVAKTSLLLFHIIFANHVGA
                     GSYGEFSFVFVGGLILLQPALDLGLNQLITKWVSRGNTEVISHSFRIKGFTALGLIPL
                     VFLIGWWMEISLLLLVTVLGFFLINTLQQSLFGILRGLEDLRPESLTVALQNVLACAI
                     LGIFVFQEIAEPWIGSLVLMLTRLAGTVFISGIIWIKYQTNLKKNESSSELLGSKTLW
                     SEAITLGLVLFLIQFYFRIDTIMLGVLASESEVGLYSVAFNLMEGTFFIPTIVMAAIF
                     PGLSQAKHFSAYFRKGVLLLTLSGIAGGSAVFLLAEWIIHLFFAAEFQSSAEILQILA
                     FAIPLVFWGYLMTQSLVALDHNRIYLGITAGGVLLNVLLNFWLIPEHGASGAAVATVI
                     TEALIPFSCFLMILKHQLSSPPAHGA"
     gene            1462..>1817
                     /locus_tag="EYO68_05670"
     CDS             1462..>1817
                     /locus_tag="EYO68_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07329.1"
                     /translation="MPLKLPALQQMLESLLSFSFPIRLSLYTLFLPPVALFCIVWNRK
                     RAVEIWQKLRLPLIVLAFLFCWMWLGAIMSEYPKVALKHSGRYSIFLMTFLAFLFAIE
                     RNSLKKSGQIFTVIYVL"
BASE COUNT          437 a          374 c          399 g          607 t
ORIGIN      
        1 tccaaaggcc tcagtccagc tacaccaatc caaaaatcgg cccttggtgg agtttgaaat
       61 acaacagcag tggagtgtgg tgcttcctga ataacaccac actgcacatg tgtttcctgt
      121 ctgtcacttc cctttaaaaa tacagaagat tccccattat gcctgtctcc gctgatttta
      181 accaagttag caatttacgc aataatctaa gttggaattt tgtaggacaa gtggtcgcta
      241 aaacaagcct cttacttttt cacattattt ttgcaaacca tgttggagct ggaagttacg
      301 gagaattttc cttcgtcttc gtaggaggac ttattcttct ccagccggct ttggatttgg
      361 ggctaaatca gctgattacc aagtgggtaa gccggggaaa tactgaagtt atttcgcatt
      421 ctttccgcat aaaaggtttt actgcactcg gattgattcc gctggtattt ttaatcggct
      481 ggtggatgga aataagcttg ctgctactgg tgacagtact gggatttttc ctgatcaata
      541 ccctgcagca atcattattt ggaattttgc gtggattgga ggatttgcgt ccggaatcac
      601 tcacggtggc cctgcagaat gttttggcct gtgcaatttt ggggattttt gtttttcagg
      661 agattgcaga gccttggatt ggttcattgg tgttgatgtt aacacggctt gcaggaactg
      721 tctttatttc cggaattatc tggataaaat atcaaacaaa tttgaaaaaa aatgaaagca
      781 gttcggaatt attaggatcg aaaacacttt ggagtgaggc gatcactttg ggattggttc
      841 tttttttaat acagttttat ttccgtatcg acaccatcat gcttggagta cttgcttcag
      901 aaagcgaagt cggtttgtat tctgtagctt tcaatttgat ggaagggacc tttttcattc
      961 caacgattgt gatggctgca atctttccag gactgtctca agccaaacat ttttccgcat
     1021 actttcgaaa gggagtactg ctgcttacgc tttccggaat cgcaggggga agtgccgtct
     1081 ttttactggc agagtggatc attcatttgt tttttgcggc ggaatttcaa agctccgcag
     1141 aaatattgca aattttggcc tttgccattc cacttgtttt ctggggatat ttgatgacac
     1201 aatctttagt tgcactggat cacaaccgca tatatctggg tatcactgcc ggtggagtac
     1261 tgctaaatgt actactcaat ttctggttaa ttccggaaca cggtgcctcc ggagcagcag
     1321 tggccacggt gattaccgaa gctcttatcc cttttagttg ctttctcatg attttaaaac
     1381 accaactttc atctccccct gctcatggtg cttaaacaat ggataaatcc actgttagtg
     1441 ggtcttttga ttttgttctc tatgcctttg aaacttccgg ctctgcagca aatgctggaa
     1501 agtttgttga gtttttcatt tccaattagg ctcagccttt acactttgtt tctaccgcct
     1561 gtggctttgt tttgcattgt ttggaaccga aagcgcgcag tcgaaatctg gcaaaaactc
     1621 cgactgcctt taatcgtact ggctttcctt ttttgctgga tgtggttggg tgcaatcatg
     1681 agtgaatatc cgaaagtcgc cctcaaacat tccggacgat actcgatatt tctcatgacc
     1741 tttctggcat ttctgtttgc cattgagcgg aattccttga aaaaaagcgg acagattttt
     1801 actgtaattt atgtact
//
LOCUS       DTVD01000164            7339 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_2951_length_7339_cov_180.408979, whole genome
            shotgun sequence.
ACCESSION   DTVD01000164 DTVD01000000
VERSION     DTVD01000164.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_7339_cov_180.408979"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(20..2482)
                     /gene="lon"
                     /locus_tag="EYO68_05675"
     CDS             complement(20..2482)
                     /gene="lon"
                     /locus_tag="EYO68_05675"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIC07330.1"
                     /translation="MSGKDQEKNTAQNQIALPMLPMRDIVVFPHMTAPFFIGRSLSIA
                     SLEKALDGDRQIFVVAQEDPLVEEPEAKDLFRVGTIGKVLQIMRLHNGTIKALFEAKS
                     RGRLIEAHMEEPHFAAVVEPIPEQVSKAPELLALSKNVRAEFKRYLKDVKKRTEGIEK
                     LSIESEEPHILADRIAPLLNMDLQKKQDLLENSDPKRRLEIVYGRMLEEKEFKKVERK
                     LKERVQGQIGRTQKEYYLNEQVKAIQKELGHGEDSKAEMDEYAKKIEEIKLSDEAREM
                     AEKELQKLKMMPSMSSEANVVRNYIDWLLSMPWAEKTEDNFDLEKAEKVLDAQHYGLE
                     KVKERIIEYLAVAQQVGKMKGPIICLVGPPGVGKTSLARSVAEALGRKFARVSLGGIR
                     DEAEIRGHRRTYIGAMPGKVIQSLRKTKFNNPLLLFDEIDKMSHGVMGDPAAALLEVL
                     DPEQNHTFMDHYLEVEFDVSDVLFFCTANVPQNIPPALKDRMEVIRLSGYTELEKENI
                     ARKHLLPKQMDENGLTSKRIQFQQKAILKIIRNYTREAGVRNLEREIAKTCRKVATQL
                     VKKTNLNKVVVTPLRVQKYLGVQSYKHGKAEEKNEIGTSCGLAWTQAGGELLVTEVNI
                     MKGTGKLQLTGKLGDVMKESAQAALSYVRTNANQLGIFSSVFEKTDIHIHVPSGAVPK
                     DGPSAGVTIATSIVSAFTSIPVRKEVAMTGETTLRGKVLAIGGLKEKLLAAKRGLIST
                     VIIPHKNKKDLSEIPEEIQSGLEIIPVQTVEKVLEIALERMPIAVIDPEPEVEENMKV
                     DSPEAAILPQTDPPESPRTYDA"
     gene            complement(2680..3000)
                     /locus_tag="EYO68_05680"
     CDS             complement(2680..3000)
                     /locus_tag="EYO68_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07331.1"
                     /translation="MRQIILFFIISQILFSGCSSTGNKFLASYDPEYKVADRGKLDVI
                     VKGRACALLADDAVLYARQTAEFHLRSVVGSQNHRKEFQEVDRYYDGDKICVEMSAAA
                     LPPL"
     gene            3231..4514
                     /gene="hemL"
                     /locus_tag="EYO68_05685"
     CDS             3231..4514
                     /gene="hemL"
                     /locus_tag="EYO68_05685"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde-2,1-aminomutase"
                     /protein_id="HIC07332.1"
                     /translation="MQITQSKQLFSRAQQRIPGGVNSPVRAFKSVNMTPLFITRAQGS
                     KLYDADGNEFIDFVGSWGPMILGHAHTEVIRKLEETLLKGTSFGAPTEGEILLAEMVC
                     EAVPSVEMLRLVNSGSEAAMAVLRVARGYTGRDKIIKFEGCYHGSVDPLLVQAGSGAM
                     TLGIPDSPGVPASFVEHTLQAKFNDLDSVIKLVEENSSKIAAVIIEPVAGNMGMIPPE
                     HGFLQGLRDLCDREGILLIFDEVMSGFRVDYGGAQALFGVLPDMSIFGKIIGGGLPVG
                     AYGGRQDIMLQVAPAGPVYQAGTLSGNPLAVAAGLSTLEILRKENPYPELGQKTSWLS
                     GEFQSAAESAGVPLQTQAMGGMFGFFFSETPVRNYQDALNSDVERFTRFFRSMLNQGI
                     YLAPSAFESLFVSTAHSEKDLERTARAFRKALQEK"
     gene            complement(4566..4862)
                     /locus_tag="EYO68_05690"
     CDS             complement(4566..4862)
                     /locus_tag="EYO68_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIC07333.1"
                     /translation="MDIETPEKGSTLHQLKFLADASHVLQAEIARTDISLQKVMQWEL
                     GSVVMFEKIVGSPIDVLIGDRLIARGEVVVVNDRYGVRINEITHPDEKPVSNWK"
     gene            5469..5708
                     /locus_tag="EYO68_05695"
     CDS             5469..5708
                     /locus_tag="EYO68_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIC07334.1"
                     /translation="MFGLGIWELLIILAIIVVLFGAKRLPLIGEGLGGMITNFKSATK
                     KNKEDQSKIDENTTVIKEDSTVSKEDVIVSKEDNS"
     gene            complement(5720..6154)
                     /locus_tag="EYO68_05700"
     CDS             complement(5720..6154)
                     /locus_tag="EYO68_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIC07335.1"
                     /translation="MGGSWEENEPLSEINVTPFVDVLLVLLIIFMITAPIVNHVIQVD
                     LPEDSYNQDVMKPLQEPLKVVIDKAGVLYLGNTRIGGSKDDESQRILQDKIRQWTKKQ
                     KQPWMVDVEADEKVDYGAIVPVIARLKELHVSMNLVIRPRPK"
     gene            complement(6129..6818)
                     /locus_tag="EYO68_05705"
     CDS             complement(6129..6818)
                     /locus_tag="EYO68_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HIC07336.1"
                     /translation="MVLSGSLIAKLVLLILALMSALSWAIIIIKALQYHNIRKEDQKF
                     YQIYLNESSLNQIKKVSSEYLFSAFSFIFSATYQEAAQMSQRIQRSSPNILESNSMLP
                     YLSQQLQRVIEKAINERYSFIENRLNILATISSAAPFIGLFGTVLGIINSFQSIGTTG
                     VTSLASVAPGISEALVATAAGLLAAIPALMAYNYFRNQARLLTNTMRNFGMEITNRFE
                     WIVNGRLLGRE"
     gene            complement(6901..>7339)
                     /gene="pal"
                     /locus_tag="EYO68_05710"
     CDS             complement(6901..>7339)
                     /gene="pal"
                     /locus_tag="EYO68_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIC07337.1"
                     /translation="KEEAQIAVEQDAEKSVEAPKAVTIISAPPEESDVAIYDLEMVYF
                     AYDKSIITTAFGEALQKNYEWIAENPDVQIQLEGHCDERGTNEYNLALGERRAKAVFD
                     YLISLGASPSQFSLVSFGEERPADHGSNEVAWRKNRRVEFTRL"
BASE COUNT         1963 a         1597 c         1503 g         2276 t
ORIGIN      
        1 cgaaccagcg acccgatgat catgcgtcat aagttcgagg tgattcaggg gggtctgtct
       61 gtggcagaat tgcagcctct gggctgtcaa ctttcatatt ttcttcaact tcaggttcgg
      121 gatcaatcac tgctattggc atacgttcaa gagcaatttc taagactttt tctacggttt
      181 gtacaggaat aatttccaaa ccactctgta tttcttccgg gatttcactc aaatcttttt
      241 tatttttgtg aggtataatc acagtagaaa ttaaaccccg ttttgctgca agaagttttt
      301 cttttaatcc gccaatcgct aaaaccttgc cacgcaaagt agtttctcct gtcattgcta
      361 cttcctttct gacaggaatg gaagtaaatg cactgactat agaagtcgca atagttactc
      421 cagcggaagg accgtctttg ggaacagctc ccgaaggaac atgaatgtga atgtcggttt
      481 tttcgaacac ggaagaaaat atccccaact gatttgcatt agtgcgtaca taactcagtg
      541 ctgcctgtgc cgactctttc atcacatccc caagttttcc cgtcaactgt aatttacctg
      601 tacccttcat aatattaact tccgttacca aaagttctcc tccggcctgt gtccaggcta
      661 aaccgcaaga ggtgccaatt tcattttttt cttcggcttt tccatgtttg taactttgta
      721 cacccaaata tttttgcacc cgcagtggag taacaacgac tttgttaagg ttcgttttct
      781 ttaccaactg ggttgcaact ttgcggcatg ttttggcaat ttcccgttca agatttcgga
      841 ctccggcttc cctggtgtaa ttccggataa ttttcaggat ggctttttgc tgaaactgaa
      901 ttctttttga tgtcagtcca ttttcatcca tctgttttgg gaggagatgt ttgcgggcaa
      961 tgttttcttt ctccagctca gtgtagccgg aaagtcggat aacttccatt ctgtctttca
     1021 gtgcaggcgg aatgttttga gggacattgg ctgtacagaa aaataagaca tccgaaacat
     1081 cgaactctac ttccagataa tggtccatga aagtgtggtt ctgttccggg tcaagtactt
     1141 ccaataaggc tgcagcagga tctcccatca cgccatgaga catcttgtct atttcatcaa
     1201 agagaagcag gggattgttg aattttgttt tccgcaaaga ttggataact ttcccaggca
     1261 tggcaccaat ataagtcctg cgatgccctc tgatttcggc ttcatcacgt attcctccca
     1321 gacttacgcg tgcaaatttt cttcccagag cttctgcaac agaacgcgcc agggatgtct
     1381 ttccaacacc aggaggtccc accagacaaa taattggacc tttcattttg cctacctgct
     1441 gagctacagc aagatattct ataatacgtt cttttacttt ttccaaaccg tagtgctgag
     1501 catccaggac tttttcagct ttttccaggt cgaagttatc ttccgttttt tctgcccagg
     1561 gcatgctgag cagccagtca atataatttc gaacgacatt tgcttctgaa gacatagagg
     1621 gcatcatttt gagtttttga agttcctttt ctgccatttc tctagcttca tcagaaagtt
     1681 taatttcctc aattttctta gcatactcat ccatttcagc tttactgtct tctccgtgac
     1741 ccaactcttt ctggatagcc ttaacttgtt cattgaggta gtattctttc tgtgtccggc
     1801 caatttgccc ctggacgcgc tcctttagtt ttcgttcaac ttttttgaat tctttttctt
     1861 caagcatccg gccatagact atttcgagcc gtctttttgg atccgagttt tccagcaaat
     1921 cctgtttctt ttgcaaatcc atgttcagaa gtggagcgat acggtctgca agaatatgcg
     1981 gttcttctga ttcaatggac aatttttcga ttccttcagt tcttttttta acgtccttca
     2041 ggtagcgttt aaattcggcg cggacatttt tactgagggc caataattcc ggtgccttgg
     2101 aaacttgttc cgggattggt tccactactg cagcaaaatg gggctcttcc atgtgtgcct
     2161 caattagacg gcctctcgat tttgcttcaa agagtgcttt aatggtacca ttatggagac
     2221 gcattatttg caaaacctta cctattgttc ctactcgaaa cagatccttt gcttcaggtt
     2281 cttcaaccaa cggatcttct tgagcaacaa cgaaaatttg acggtctccg tcaagtgctt
     2341 tttccagtga ggcaatagag agtgagcggc cgatgaaaaa cggtgcggtc atgtggggga
     2401 acacgacgat atcacgcata ggcaacattg gcagcgcaat ctggttttgt gcagtatttt
     2461 tttcctggtc ttttcctgac ataaactacc tttaaattgg ctaagtgagt gaagtaggaa
     2521 aacattcctg aatcttgaga accgaaagaa tccagacttt atgtttaatc ttagttcata
     2581 aatcgtaaac ttccaagtcc agaaacttat tattacttta tagatttgca gcttaaatcg
     2641 caagtaatca aggttctaag gcaaaattat taatccgctt cataaaggag gcaaagcagc
     2701 agcactcatt tccacacaga ttttgtctcc atcatagtag cgatctactt cctgaaattc
     2761 cttcctgtga ttctgacttc ctacaacgct gcgcaggtga aattcagctg tttgccttgc
     2821 atacaagacg gcatcatccg caagaagtgc gcaagccctg cctttaacta tgacgtcaag
     2881 cttaccccga tctgcaacct tatattccgg atcataagaa gccagaaatt tatttccagt
     2941 agaggaacat ccactgaaca gaatttggct aattatgaaa aacaggatta tttgtcgcat
     3001 tttttagtga agtcttgaca actcttgagt cgttctccaa actatttttt acattaatta
     3061 cgttcataga atatatattt ttggattcag gtaccttaaa gttggtaaca gaacataaac
     3121 agtttccgaa ttttatctgg tctgttctca ggatccactc aaaccggaat agccggaaac
     3181 ttattcttct atgaaaaact cttgatcaaa ctttaagaag tgcaaccttc atgcagatca
     3241 cacagtctaa gcaattgttt tctcgcgccc agcaaagaat acctggaggc gtaaacagtc
     3301 ctgtccgtgc tttcaaatca gtaaacatga caccgctttt tatcacccgt gctcagggtt
     3361 caaaactgta tgacgcggat ggtaatgaat ttattgattt tgtcggctcc tggggaccta
     3421 tgattttagg acacgcacat acagaagtta tcagaaaact tgaagaaact ctactgaaag
     3481 gaacttcttt tggtgctccc accgagggag aaattttgtt agccgaaatg gtctgcgagg
     3541 cggtgcccag tgttgaaatg ctgcgtttgg ttaattccgg ttctgaagcc gctatggcag
     3601 ttcttagggt tgcccgtgga tatacaggcc gagataaaat aattaaattt gagggttgct
     3661 accacggcag cgtggacccc ttactcgtac aggccggatc cggagcaatg actttaggaa
     3721 ttccggattc tccgggagtt ccagcatctt ttgttgaaca tacgctgcag gcgaaattca
     3781 atgatctgga ttctgtcatc aaactggttg aagaaaactc cagtaaaatt gctgcagtta
     3841 ttattgaacc tgttgcaggt aatatgggca tgattcctcc tgaacacgga tttttacagg
     3901 gcttgcgtga cttgtgtgac cgcgagggta ttttgcttat ttttgatgaa gtgatgagtg
     3961 ggttccgggt tgattacgga ggtgctcagg cactgtttgg ggtgcttccg gacatgagta
     4021 ttttcggaaa aattatcgga ggcggactcc ccgttggcgc atatggtgga cgtcaggata
     4081 tcatgcttca agttgcacct gccggccccg tttatcaggc tggtacgctg tctgggaatc
     4141 ctctggcagt agccgcaggt ttgtcgactt tggagatttt aagaaaggaa aatccttacc
     4201 ctgaacttgg acaaaaaaca tcatggcttt caggagagtt tcaatcggca gcagaatcag
     4261 ctggagtccc gctgcagacg caggccatgg gtggtatgtt cggatttttc ttttctgaaa
     4321 ctccggtacg taattaccag gacgcactca actctgatgt ggagcgcttc acccggtttt
     4381 tccgcagtat gctaaatcag gggatttatc tggcaccctc agcttttgaa tcgctttttg
     4441 tttctaccgc tcattctgag aaggatctgg aacggacagc cagggcattt cgtaaggcac
     4501 tgcaagaaaa atgatttaac tgatattggc aaagtcttac cgtaacgtcc acctgtaatc
     4561 agtgattatt tccagtttga aactggtttt tcatctggat gagtaatctc attgattcgc
     4621 actccgtaac ggtcatttac cacaacaacc tcacctctgg caattaaacg gtcaccaatt
     4681 aacacatcaa tcggtgatcc aactattttc tcaaacatca ctacagatcc tagttcccat
     4741 tgcataactt tttgcaggga tatatctgtc ctggcaattt cagcctgcaa aacatgcgaa
     4801 gcatctgcta aaaactttaa ctgatgaaga gtggatccct tttctggagt ttcaatgtcc
     4861 atggacggtt ttgtaaagtt tgctaaatat taattgcctg acaaaaaatt gaaatttttt
     4921 ttcccagcag tgtgagaaac agtttatttg attacagaaa aaaatatcta taacttgctg
     4981 aataataata ttattcaata atatcaataa gtaattatca gatttatgat gacaaccgca
     5041 aggtttttca tgaaaaagta ctaaaagctt ttcacttctg ttgtttgagg cccttttcca
     5101 acaaggtgag cccacgtgaa aaatagtcgt acttctttcg attcttctcc tttgccattc
     5161 tgggcaaagc gaagcgcaga cccggaatca aggggaaatg tttcaaatct tgtactcaag
     5221 ctgatcggtg atccggatcc cgaatcatcc gggatgacaa aagaaatact ttttcagtga
     5281 gccctatcta aacaggtcta gatgacaata taacctaatt ttcaatttta ttcatgaact
     5341 acatttatta acagttgact tgttttatta aacttgcgat tatcagtcca gtttgatcta
     5401 ctgttagtta caattcacat tatagggtcg tctatgacca atgacacaat tagttaggag
     5461 agtgcactat gtttggttta ggtatttggg aacttttgat tattttggct attatcgtag
     5521 ttttgtttgg agcaaaacgc ttacctctga ttggtgaggg acttggcgga atgattacca
     5581 attttaaaag tgcaacaaag aaaaacaaag aagatcagtc aaaaattgat gaaaatacta
     5641 ctgtaattaa agaggactct actgtcagca aagaagatgt aattgtcagc aaagaagata
     5701 atagctgaat gaattatctt tattttggtc tgggacgaat aaccaggttc atgctgacgt
     5761 gcaactcttt gagtcgggca atgactggaa cgattgcacc gtagtcaact ttttcatcag
     5821 cttctacatc aaccatccac ggctgtttct gcttcttagt ccattgtcga attttatcct
     5881 ggagaatccg ctgactttca tcgtctttac tgccgccgat acgcgtattt cctaaataca
     5941 gtacccctgc cttatcaatc accactttta gcggttcctg caaaggcttc attacatcct
     6001 ggttgtagct gtcttccgga agatccacct gtatcacatg atttacgatt ggagccgtta
     6061 tcataaagat gatcaacaat accagcagta cgtcaacaaa tggtgtaacg ttgatctcac
     6121 tcagtggctc attctcttcc caggagcctc ccattcacta tccattcaaa acgattagtt
     6181 atttccatgc caaaatttcg catggtatta gttaatagac gagcctgatt gcgaaaataa
     6241 ttgtaagcca tcaatgcagg aattgcggca agcaatcctg cagcagttgc aaccagagcc
     6301 tcagaaatac ctggtgcaac tgaagcaaga cttgttactc ccgttgttcc aatgctctga
     6361 aaagagttga taattcctaa gacagttcca aataatccaa tgaagggtgc tgcactagaa
     6421 atggttgcaa gaatatttaa acggttttca atgaaagagt accgttcatt gattgccttt
     6481 tcaatcactc gttgcaactg ttgactgaga taagggagca tcgaattgct ttctaatatg
     6541 ttaggagaag aacgctgaat gcgttgactc atttgcgctg cctcctgata ggtggcagaa
     6601 aaaatgaatg aaaaagcaga aaaaagatac tctgaagaaa cttttttgat ttgattcagg
     6661 ctggactcgt tcaggtagat ttgatagaac ttttggtctt ctttgcggat gttgtggtac
     6721 tgaagcgcct taattatgat gatggcccag gaaagggccg acatcaaagc caaaatgagc
     6781 agcaccagct ttgcaatcaa actgccactc agtaccatcc cgactatgct cgtttctcct
     6841 aaccattcgg tgagcataat tcaaagagta gtcgaaattc gtgacagagt tacaaagtgt
     6901 ttacagacgg gtgaactcta ctcgcctatt ttttctccag gcaacttcat tacttccatg
     6961 atctgcagga cgttcctcgc cgaaactgac caatgaaaat tgtgatggac tggctcccag
     7021 gctgatcagg tagtcaaaga cagcttttgc acgccgttcc cccaatgcca gattgtattc
     7081 attggtacct cgctcatcac agtgaccttc caattgaatc tggacgtccg gattttcagc
     7141 aatccattca tagtttttct ggagagcttc tccgaaggca gtagtgataa tactcttgtc
     7201 gtaggcaaag tagaccattt ccaaatcata aattgccaca tctgattcct ctggaggagc
     7261 tgagatgatg gtgacagctt tcggagcttc aacacttttt tcggcatctt gttctaccgc
     7321 aatttgagcc tcttctttt
//
LOCUS       DTVD01000165            1813 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_29559_length_1813_cov_221.004551, whole genome
            shotgun sequence.
ACCESSION   DTVD01000165 DTVD01000000
VERSION     DTVD01000165.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29559_length_1813_cov_221.004551"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
BASE COUNT          511 a          344 c          369 g          589 t
ORIGIN      
        1 tgtgtcttca ctccagaaaa gacgtccatc aaaagcggaa gatgaaaaat ttcattcaca
       61 acttgacagc agccacaaag cagccaagct gcgtatgtcc tcttctgatg aaaagctaca
      121 caccttccat acaccagtag aattctcctg gtcccagttc tggatagctt ttatttatga
      181 gaacctacct ccggtctttg tttcccctat tgctgccctg ctcattgagc ggtcttttac
      241 cagagcctgg catgtcactc agcaccggtc tctatgctta gtttcaacca aacacaattc
      301 attggggttt attatttttt cctgggtcat tatctatcca tcttcctggc ttatcactgt
      361 tgcattgact ctgaggttat ttggatacga aggagcagtg cagaatgttg acctttttca
      421 gatgatactt gcataccttt tcctgtttat gcgtcgtctg attatttcgg taaaatatgc
      481 gtattttaga ccagaggata tagtactact aggtctttca gcacctaact gggatggcga
      541 taaaacaagc cgacgattag tggggttcgg ttggagcaac ccagctagtt accctgggct
      601 cattgaggac gaactgacct gtgccatgga tgaaaatgat ctttcactgc aggggattac
      661 ctttaaatta gatgagagtg tctgcaattc aatgaagaac catcctacca acgagctatt
      721 tacagctgaa aagaaaaaca atcaaagaga tgaagtaaac tcaggtttca ttcttcatca
      781 aattttacgt gcagtctaca acatcaaatt acccccaatt tttaatttaa tacccatgtc
      841 gtgcgcattt tctatcgcat tgctcccgat ctttttaaga atgtattatg gattggatgt
      901 tttcggtgaa accttttttg aaaatgttat atttgctgga tgttttatag gatttttaaa
      961 tggtttaaat attatgactt ttggatttat ctgtgcctat gatttcaaaa ggagattcaa
     1021 tgcaatgaaa agattgggtg aattaataaa tttcccagga attcctttaa gtgagttcct
     1081 ttttcatatg cctaaaaagg aaattaagaa tagtggtgag caacctgatg aaaatcttag
     1141 tgctgatatt gagaatgacg aacttggggg accccagaaa accccttatg tttttatcga
     1201 ccttaagaag cgttcgaacg tttttgcctg gatgaattgc cgcaaaacct tgcgctcttt
     1261 cggagaggga ttttatcttc ggattcaagg ctacacttcc atcctgttaa tgtactcttt
     1321 cttttgtgtt gtcattctaa atgtaattgc ctggatgcag atgaggcacc atgtttcaac
     1381 aatttatttg ataatgatga tagtgattat tatagcttca attagcatct tctcaatttc
     1441 taaagcaata aaactgcagt acttaagttc gagccagcga gatttgatca ggaaagaaat
     1501 tttccttcag gaagaggaaa tatggaattc agaatctgaa gataatagtg ccaaggagct
     1561 aaggcaactg agaagcgcca aggctttatt ggagcaggtg gacgagtcta ttaattttaa
     1621 tgatttaatc cacaaaccta ccaaggttct ggggtatgtg gcaaaccaaa atgtgattgg
     1681 ctctgcgttg ggtattatag ctaccggttg ttttttagca attgaaggat tttcaggatc
     1741 aggtatctca tatgaccaaa acggttggtt taatttttag ctgtttgctt ctgcatctgc
     1801 aaggtgccgt tct
//
LOCUS       DTVD01000166            1809 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_29675_length_1809_cov_229.268529, whole genome
            shotgun sequence.
ACCESSION   DTVD01000166 DTVD01000000
VERSION     DTVD01000166.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29675_length_1809_cov_229.268529"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="EYO68_05715"
     CDS             complement(<1..194)
                     /locus_tag="EYO68_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HIC07338.1"
                     /translation="MNYEVIVSCAVTGAGDTVGKSPLIPVTPEEVANAAIEAAKAGAS
                     IAHIHVRDPETGKGSRDPEL"
     gene            complement(237..620)
                     /locus_tag="EYO68_05720"
     CDS             complement(237..620)
                     /locus_tag="EYO68_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HIC07339.1"
                     /translation="MDKERFERGLAARKSVLGAEYVEKALANADDFNREFQEQLTEFC
                     WGSCWGNETLDRRQRSLLNLGMIAALNRMVEWEIHFRGAIKNGITREELKAILLQISV
                     YCGMPTGVECFRIAQKVFAELDAVE"
     gene            complement(649..>1809)
                     /locus_tag="EYO68_05725"
     CDS             complement(649..>1809)
                     /locus_tag="EYO68_05725"
                     /inference="COORDINATES: protein motif:HMM:PF03972.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIC07340.1"
                     /translation="TSRTVFWMGASMHIQEVDDLHRQSVVHPGCVVIPTVLALGMRED
                     ISGLQMLEAVVKGFEACTRIGNSVGPAHYKIWHNTATCGPFGAAYAAGTLFGLEKEQF
                     RDALGNAGTQSSGLWEFSENGAMSKHLHAGRAGQSGLLSAELAKLGFSGSPTILEGKR
                     GFYAACCPDANPDALLVDPEGSWQIHKTSIKPWPCCRHTHPAIDAALEISSKLDGGNI
                     ESIELGVTQATLDVCDKPTPETLYEAKFSLQHCVSVAILDGQVGFDAFESDARRRVAK
                     MAMKISLERNEEIEKAYPNAWGAEVKVKLTNGKEFSVRRKHAKGDPDAPLSPTEFKEK
                     ATMLFRFGGVSEPEAWVERILELDRVSSFRDSDLIDLLDVSANGSMGKSFKI"
BASE COUNT          447 a          472 c          400 g          490 t
ORIGIN      
        1 aacaattccg gatcgcgtga tccttttccg gtttccggat ctcgaacatg aatgtgcgcg
       61 atcgacgcac cggctttggc tgcttcaata gcggcattcg caacttcttc tggtgttacc
      121 ggaatgaggg gacttttccc gacggtgtcg ccggcccctg tgactgcaca tgaaacgatt
      181 acttcataat tcataattca acctcttaat cataagtttt tcaaaataaa ttttgttcac
      241 tcaacagcat ctagttcagc aaacactttt tgggcgatac ggaagcactc cactccggtg
      301 ggcattccac agtatactga gatttgaagc aaaatggcct tgagttcttc gcgtgtgata
      361 ccattcttga tagcaccccg gaagtggatt tcccactcaa ccatgcggtt gagtgccgca
      421 atcataccca ggttgagtaa gcttcgctgg cgtcggtcta atgtttcgtt cccccagcaa
      481 gaaccccaac agaactctgt gagttgttcc tgaaattctc ggttaaaatc gtcggcattc
      541 gccagcgcct tttccacata ttcagctccg agcacagatt ttcttgcggc aagtccgcgt
      601 tcaaaacgtt ctttatccat atttatttct tggtttgagt ttgttagttt aaatcttaaa
      661 agatttgccc atgcttccgt ttgcggaaac atccagcaag tcaattaagt cactatcacg
      721 aaacgatgac acacgatcga gttcaagaat gcgttccacc catgcctcag gttcggaaac
      781 tcctccgaaa cggaaaagca tggttgcttt ttccttaaat tcagtcggtg aaagcggggc
      841 atccggatca cctttggcgt gtttccgacg gacgctaaat tcttttccat tcgttaattt
      901 aactttaacc tctgcacccc aggcattcgg gtaggccttt tcaatttctt cgttgcgttc
      961 cagactgatt ttcatcgcca ttttggcaac ccgtcggcga gcatcggatt caaaagcatc
     1021 gaaaccgact tgaccatcaa gtatggcgac agagacacaa tgctgcaagg aaaacttggc
     1081 ttcgtaaagc gtttccggag ttggtttatc acagacatcg agggtagcct gtgtgactcc
     1141 aagttcgatg gattcaatat ttccgccatc caacttactt gaaatttcca aagccgcatc
     1201 aatagccgga tgtgtgtgcc gacaacaggg ccagggttta attgaagttt tatgaatttg
     1261 ccaggaacct tccggatcaa caagcagagc atccggattt gcgtcgggac agcaagccgc
     1321 ataaaaaccg cgtttgcctt ccaaaatggt gggtgaaccg ctgaaaccaa gtttggccag
     1381 ttctgcggaa agtaatccgg attgtcccgc acgacccgca tgaaggtgct tactcatcgc
     1441 accattttct gaaaattccc agagcccgga ggactgtgtt ccggcatttc caagagcatc
     1501 ccggaattgt tctttctcaa gtccaaagag tgttcccgcc gcataagccg caccgaaagg
     1561 accgcaagtt gcggtgttat gccaaatctt gtaatgcgcc ggacccactg aattaccgat
     1621 cctcgtgcag gcttcaaatc ctttgactac tgcttccagc atctgcagtc cgctgatgtc
     1681 ttcacgcatt ccaagtgcaa gtaccgtcgg aatgaccaca catcccggat gaaccactga
     1741 ttgtcggtgc agatcatcta cttcctggat atgcattgat gcgcccatcc agaaaaccgt
     1801 gcgtgaagt
//
LOCUS       DTVD01000167            7234 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3028_length_7234_cov_514.419975, whole genome
            shotgun sequence.
ACCESSION   DTVD01000167 DTVD01000000
VERSION     DTVD01000167.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3028_length_7234_cov_514.419975"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            14..238
                     /locus_tag="EYO68_05730"
     CDS             14..238
                     /locus_tag="EYO68_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:122..124,aa:Sec)
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07341.1"
                     /translation="MNENSLARPSGFENLTSILSCPLCRRASEEIVPPDTUIRMHRCR
                     SCSKDFAAKPGDCCVFCSYGSVRCSGELNL"
     gene            complement(318..1211)
                     /locus_tag="EYO68_05735"
     CDS             complement(318..1211)
                     /locus_tag="EYO68_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIC07342.1"
                     /translation="MLILCSPLEAAEESPVNLAPNISAGKYLFQAAGGCSCHTDTKNN
                     SAFLAGGRPIETPFGTFYGTNITPDPETGIGNWSDEDFIRAMTQGLSPEGNHYFPVFP
                     YTSFQRISRKDLLALKAYLFSLPPVRQKNLPHDLILPFGEQYAMMFWKNFVWSPEPFI
                     SNPERTESWNRGEYLAQALAHCGECHTPRNLLGALKPEMHFAGSKEGPEGELAPNITP
                     DKETGIGSWNKVDISYFLQNGMKPDGDDVQGLMGEVIELGYQHLLEEDLDALAEYLLS
                     LTPISNELRPDSAENSETEEY"
     gene            complement(1294..1770)
                     /locus_tag="EYO68_05740"
     CDS             complement(1294..1770)
                     /locus_tag="EYO68_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07343.1"
                     /translation="MKLFQFLVLIFLLFQLNFGVALGNPDSSDSDSNDDSKPVNEAYQ
                     NAYYEVKSGNFQVAIKYLKQAAKSSTNKADIYNLMGYSHRKLDLLEEAFFYYQKALKL
                     DPRHQGANEYIGELYLRTNNLKKAEEHLEVLDDVCLFGCDEYDDLKDAIEKYKKSM"
     gene            complement(1780..2232)
                     /locus_tag="EYO68_05745"
     CDS             complement(1780..2232)
                     /locus_tag="EYO68_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIC07344.1"
                     /translation="MKQIKKVAALTVLLSFCFFVSSSLADSEGIIKYRKNVMKSSGGH
                     MGAIVDILKNDLPLQAHILDHARSINQNSKMILEMFPIGSGLGDTKAKPAIWENWSKF
                     ESAVNAFVRESSKLVKVAESGDMKALAKQFRATGKTCGGCHKNFRERE"
     gene            complement(2374..2682)
                     /locus_tag="EYO68_05750"
     CDS             complement(2374..2682)
                     /locus_tag="EYO68_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIC07345.1"
                     /translation="MFKAMILLKRKEEYTQREFRNWWLGEHAQLAIQLPGLRKLCFNP
                     TDNEEAMYDGVAELWFDSEADFTEAYQTELGKAVAADSLAHVGRRDRLFVDENILLDD
                     "
     gene            complement(2705..3250)
                     /locus_tag="EYO68_05755"
     CDS             complement(2705..3250)
                     /locus_tag="EYO68_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIC07346.1"
                     /translation="MKPQIRRIVIQVEEIHQEIGRTIDPPARKVTVAAVINNPYAGKY
                     VDDLEPLYDLGAETGGLLAKKGVTALGVKPSEIVSYGKGAIVGLDGEIEHAAAILHPR
                     FGKPVRAAVEKGDDIIPSTKKMGGPGSVLVMPLTNKDSIWSFDHMDATEISLADAPRA
                     DEIVVAVALADSGRPLARTGT"
     gene            3470..4969
                     /locus_tag="EYO68_05760"
     CDS             3470..4969
                     /locus_tag="EYO68_05760"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIC07347.1"
                     /translation="MQIEANNYTIAVLGAGTMGEGIAQIAGTAGHPVLLYDVSQEQTQ
                     SAIENIDKRLQRSVNKEKITDKKRTQILANIKPVQTFEELAKAKLAIEAIIEDLLIKQ
                     DVFSQLESLLEEDAILATNTSSIDLNEMGSVLKRPERLVGMHFFNPAPVMALVEVVQS
                     TETDSEVADMVFSLAEQWGKNPVHVRSSPGFIVNRAARPFYSEALAILKEGGTDAATC
                     DAIMRECGGFRMGPFELMDLIGLDVNYAVTCQIWESYQRHPRFAPSVLQKELVDSGRL
                     GRKSGQGFFEYGVDVEMQAPKNAPESPAPASLIIEGPEKLPQSLLKLIEGGSLKTKSI
                     SGNGIIRLPAGAAVMISNGKSSTEQSLDLEENVISLDLCLDYFQSPRIALAPASQCSA
                     NALQEAVGLFQSLGKLVSVLKDIPGMVLTRTVAMLSNEASLLVQEEISDAEGVNLAMR
                     KGVNYPQGPLEWAEQWGLFSVVETLDNLRKFYGERYQVSSWLQQKAKHN"
     gene            5163..6368
                     /gene="pcaF"
                     /locus_tag="EYO68_05765"
     CDS             5163..6368
                     /gene="pcaF"
                     /locus_tag="EYO68_05765"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIC07348.1"
                     /translation="MEEAFICDGIRTPIGRYGGALAGVRTDDLAAIPIKTLMERNSGV
                     DWETVNDVILGCANQAGEDNRNVARMAALLSGLPQTVPGATVNRLCGSGLDAVGIAAR
                     AIKSGEQNLVIAGGVESMSRAPFVMGKATSAFSRNAETFDTTIGWRFVNKLMLEQYGT
                     DSMPETAENLAAEFNISREDQDAFAWRSQQKAGQAMESGRLGEEIVPVSIPQLKGEPL
                     IVDQDEHPRPETKLEKLGKLTTPFRENGCITAGNASGVNDGAAALIIASETMATKQKL
                     SPVARVVGMATAGVEPRIMGYGPVPATQKLLELTGVSLDKIGIIELNEAFAAQALAVT
                     RALDLADDDQRVNPNGGAIALGHPLGMSGARLALTAVKELQLRRERYALCTMCIGVGQ
                     GIAMLIERV"
     gene            complement(6396..7229)
                     /locus_tag="EYO68_05770"
     CDS             complement(6396..7229)
                     /locus_tag="EYO68_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07349.1"
                     /translation="MKLNVINSSSGQNNTIFAATGGRVLNPELPLVIFMHGGGMDHTV
                     WNLHTRYFAFHGYSVLAFDFPGHGRSDGSFLDSIEQMADWIPELMNALGLEEPYHTAS
                     LVGHSMGALAALECTSRYPERVRSLCLMGPSAKMPVHPDLLEAARKDDPLAYDLVTSW
                     GHGPAGHLGRTPVPGLSLIGGGRALLSSAPKGALGNDLEACNVYQNGMNAAKNIRCPT
                     LCIIGSDDKMTPARNGMELASTIPEAKTEIIQNCGHMMLLEDSNASLKALKGHLNSFE
                     S"
BASE COUNT         1977 a         1589 c         1647 g         2021 t
ORIGIN      
        1 tcacaaacta aaaatgaatg aaaactcatt agctcgacca agtggatttg aaaacctaac
       61 ttcaattctc tcctgcccgc tttgccgacg cgcctcagag gaaattgttc ctccggatac
      121 ttgaatccgg atgcaccgct gcaggtcatg cagcaaagat tttgcagcaa aacccggaga
      181 ctgctgtgtg ttttgttcct atggttcagt caggtgttcc ggtgaactaa acttgtagtc
      241 gacagaatca agctaagacc attttttaac aaatcctgga accactggcg aatacgaaaa
      301 gcaaaagtat agtgaaatta gtattcctca gtttcagaat tttctgctga atccggccgc
      361 agttcatttg aaattggagt aagtgaaagc agatattcgg ccagcgcgtc caaatcttct
      421 tccagcagat gctggtagcc cagctcaata acttctccca tcaaaccctg tacatcgtca
      481 ccgtcgggtt tcattccgtt ttgtaaaaaa tagctgatat caaccttgtt ccagctgccg
      541 attcctgttt ctttgtccgg agtgatattc ggcgcaagtt caccttccgg accttctttt
      601 gatccggcaa agtgcatttc cggtttaagc gcacccagca gatttcgcgg agtatgacat
      661 tctccgcaat gggcaagtgc ttgagcaaga tactctccgc gattccaact ttctgttcgt
      721 tccggatttg aaataaaggg ttctggagac cagacaaagt ttttccaaaa catcatcgcg
      781 tattgctcac caaaaggcag aatcaggtcg tgaggcagat ttttttgacg aacgggaggt
      841 aatgaaaaaa ggtaggcttt gagggccagc aaatccttgc ggctaatccg ctggaaagaa
      901 gtgtaaggga acaccgggaa atagtgattt ccttcaggac tcaagccctg cgtcatggcc
      961 cggataaaat cctcatcact ccagtttcca atgccagttt caggatcggg ggtgatgttt
     1021 gttccgtaaa aggttccgaa aggcgtttca atcggtcggc ctccggcaag aaaagcactg
     1081 ttgttttttg tgtcagtatg gcaactgcag cctccggcgg cttgaaaaag atatttgccg
     1141 gcagaaatgt ttggtgctaa attaacagga ctttcctcag cggcttcaag tgggctgcag
     1201 agaatcaaca aataccaaca tactaaagca cacaatcggc ggctgttcac taagtatttt
     1261 ccccggaatg tctgcggctt ccaccaacaa tttctacatg ctttttttgt acttttcgat
     1321 ggcatctttg agatcgtcat actcgtcgca accgaataaa cagacatcat caaggacttc
     1381 caaatgctcc tcggcttttt tgaggttatt ggttcgcaaa tagagttcac caatgtattc
     1441 attcgcacct tgatgacgcg gatccagttt cagtgccttc tggtaataga aaaaggcctc
     1501 ttccagcaga tccagttttc ggtgactgta tcccatcaaa ttatagatat ccgctttatt
     1561 tgtggagctt ttggcggcct gctttaaata tttgatggca acctggaagt ttccactttt
     1621 aacctcataa taagcatttt gataagcctc gttcacgggc ttggaatcgt cattgctgtc
     1681 tgaatctgaa gaatccggat tccctaatgc cactccgaaa ttcaactgga acaagagaaa
     1741 aatcaaaact aaaaattgga aaagtttcat ctgatttcct tattctcttt cgcggaaatt
     1801 tttgtgacag ccaccacacg ttttacctgt tgcacggaat tgttttgcta aagctttcat
     1861 atcgccgctt tctgcgactt tgaccagttt agaactttca cgcacaaaag catttactgc
     1921 ggattcaaat tttgaccagt tttcccagat tgccggtttt gctttggtgt ctccgagtcc
     1981 ggaacctatt ggaaacattt ccagtatcat tttgctgttt tgatttatcg accgcgcatg
     2041 gtcaagaatg tgggcttgca gaggcaaatc gtttttcaaa atgtccacaa tcgctcccat
     2101 atgacccccg gatgacttca tcacgttttt gcggtacttg attataccct ccgagtcagc
     2161 caaagatgag ctgacaaaaa aacaaaatga caacaaaact gttaatgctg cgactttttt
     2221 tatttgtttc atagatcaat cctccagata attattaata attttaaaag ttcaatactg
     2281 aacgtaacat atcatggtga agtaattaaa acaacaaaaa tagtttagac tattagaatt
     2341 ggcataagac tgaaaaaaat tgtttgcgaa attttaatcg tctaacagta tgttttcatc
     2401 cacaaataaa cggtccctgc gtccgacatg tgccaaagaa tcagcagcaa ctgctttgcc
     2461 gagttcagtt tgataggcct ccgtaaaatc tgcttctgag tcaaaccaga gctctgccac
     2521 tccgtcatac atagcctctt cgttatcggt gggattgaaa cacaatttgc gtaatccagg
     2581 cagttgaatg gcaagttgtg catgttcacc gagccaccag tttcggaatt cgcgctgagt
     2641 atattcttct ttgcgcttga gtaaaatcat tgctttaaac attttcttta tccttttata
     2701 tttgttatgt tccggttcta gcgagtggac gcccgctgtc agcaagagcg actgccacca
     2761 ctatttcatc tgccctgggt gcatccgcaa gactgatttc ggtggcatcc atatgatcaa
     2821 aactccagat gctgtctttg ttggtcaacg gcattaccaa aaccgatcct gggccgccca
     2881 tttttttagt agaaggaatt atatcatcac ctttttcaac tgcagcacgt accggtttac
     2941 caaaacgagg atgtaaaatg gctgcggcat gttcgatttc tccgtccagt ccgactattg
     3001 cacctttgcc gtaactgaca atttcggaag gttttacgcc tagtgcagta actccttttt
     3061 tagccagcag tccacctgtt tccgcaccta aatcgtaaag cggttccaaa tcatcaacat
     3121 atttacctgc atacggatta ttgattactg cagcaactgt gacttttcgt gccggtggat
     3181 caatcgtacg gccaatttcc tgatgtattt cttcaacttg gatgacaatt ctgcgtatct
     3241 gaggtttcat gtaattcctt tgatttgtta tgctgaaaat attacttttt agcgacggcc
     3301 ttctaccggg actctaatgg aaaattatct gtttttcaag ctcttttaag aaagatggtc
     3361 gaattgtttt tcaaattgca tgagtttgtt tgatcggcta aaataacccg acgttaaatc
     3421 tgtttatact ttttaacttc cattctatcc cgtcataaat aaaaatcaaa tgcaaattga
     3481 agcaaacaat tataccatcg ccgtgcttgg tgcaggaacg atgggtgagg gaattgccca
     3541 gattgcggga acggctggac atccagtgtt gctttatgac gtctctcagg aacaaacaca
     3601 aagtgcaatt gaaaacattg acaagcgact ccaacgctca gtaaataaag aaaaaattac
     3661 ggacaaaaaa agaactcaaa tcctggcaaa cataaagccg gtacaaactt ttgaagaact
     3721 tgctaaggcc aaactggcga ttgaagcgat tattgaggat ttgcttatta aacaggacgt
     3781 tttttcccaa ctggaatcat tgctggaaga agatgcaatt ttggcgacca atacttcatc
     3841 gattgattta aacgaaatgg gcagtgtgct gaagcgtccg gaacgtctgg tcggcatgca
     3901 cttttttaat cctgctccgg taatggcgct ggtggaagtt gtgcaatcca ctgaaacgga
     3961 ttcagaggtt gcagatatgg ttttctcact cgcagagcaa tgggggaaaa acccggtaca
     4021 tgtccgctcc agtccgggat tcattgtcaa ccgcgcagcc cgcccttttt acagtgaagc
     4081 tctggctatt ctcaaagaag gtggaactga tgcggcaacc tgtgatgcca taatgcgtga
     4141 atgcggtggt ttcaggatgg gaccttttga actgatggat ttgattggcc tggatgtgaa
     4201 ttatgcggtg acctgtcaaa tttgggaaag ttatcagcgt catcctcgtt ttgccccctc
     4261 tgtgctgcaa aaagagttgg tggattccgg acgtttagga cggaaatcag gacagggatt
     4321 ttttgaatat ggagtggatg tggaaatgca ggccccaaaa aatgctccgg aatctcctgc
     4381 tcctgcttct ctcattattg aaggccctga aaagctgccc cagtctttac taaaattgat
     4441 tgaaggaggt tctttaaaga caaaaagcat ttccggaaat ggaattattc gcctacctgc
     4501 aggtgcggca gtcatgattt caaatgggaa aagctcaact gagcaatctc ttgatttgga
     4561 agaaaacgtg atttcactgg atttatgttt ggattatttt cagtccccgc ggattgcgct
     4621 tgctccggct tcacagtgtt cagcaaacgc tttacaggaa gcagtgggtc tttttcagtc
     4681 tttgggcaaa ctggtatctg tattgaaaga tattcccgga atggttctga cacgtactgt
     4741 tgccatgttg tccaacgaag cttctttgct cgtccaggaa gaaatctccg atgccgaagg
     4801 agttaatctg gccatgagga aaggagttaa ttatccgcag ggacctttgg agtgggccga
     4861 gcaatggggc ttattttcgg tggtggaaac ccttgataat ttacgtaagt tttatggaga
     4921 acgttatcag gtctcttcct ggctgcagca aaaggcaaag cataattaac aatgctttcg
     4981 ccacttaact attgtcattc cgggcgaagt gaagcgtaga ttcggaatcc aggggaattt
     5041 cttcaaatct tgtattcaag ctgaacggag atctggatcc cggatcaagt ccgggatgac
     5101 aaaagaaaaa tctttttcag tgagccctaa ctaaaaacaa aatcaacaat taaaaaatca
     5161 aaatggaaga agcttttata tgtgatggaa tccggacccc gattggacga tacggtggtg
     5221 ctttagctgg agtgagaact gatgatttgg cggcaattcc aataaaaaca ttgatggaaa
     5281 gaaattccgg agtggattgg gaaacagtga atgatgtaat tctgggctgt gcaaatcagg
     5341 ccggagagga taaccgcaac gtggccagga tggcagcttt actttccgga ttgccgcaaa
     5401 ctgttccagg agcaaccgta aaccgtcttt gtggttccgg gcttgacgca gtggggattg
     5461 ccgcaagagc aatcaaatcc ggagaacaaa atctggtcat tgccggcgga gttgaaagca
     5521 tgagccgggc tccttttgtg atgggaaaag caacatccgc attttcacga aatgctgaga
     5581 cttttgacac aactatcggc tggcgctttg tcaataaact gatgctcgaa caatatggaa
     5641 ctgattcaat gcctgaaact gcggaaaatt tggctgcaga atttaatatt tcaagagagg
     5701 atcaagatgc ttttgcctgg cgttctcagc aaaaagccgg acaggcaatg gaatcaggac
     5761 ggcttggaga ggaaattgtg ccggtaagta ttccgcagct caaaggtgag ccgttgatcg
     5821 ttgatcagga tgaacatcca cgtccggaaa caaagctgga aaaactcgga aaattgacca
     5881 cgccctttcg tgaaaacggt tgcatcactg cgggaaatgc ttccggagtt aatgatggtg
     5941 cagcagcttt gatcattgca tcagaaacga tggcaacaaa acaaaagctt tctcctgttg
     6001 cgagagtcgt aggaatggcc acagcaggtg ttgaacccag aattatgggc tatggtcctg
     6061 ttccggcaac gcaaaaactg ctcgaattga ccggagtttc actggacaaa atcggaataa
     6121 tcgaactcaa tgaagcattt gcagcgcaag ctttagctgt aacgcgtgct ttggatttgg
     6181 ctgatgacga tcagcgtgta aatccaaatg gaggagcaat tgcgctggga catccgctcg
     6241 gtatgagtgg cgcccgtttg gctttgaccg ccgtgaaaga actgcagttg cgcagagaac
     6301 gttatgcgct ttgcacaatg tgtatcggtg tcggacaggg aattgctatg ctgattgaac
     6361 gggtctgaga tagacctctg aaacaggtgc tgagatcagc tttcaaaact gtttaaatgt
     6421 cccttaaggg ccttcaaaga tgcatttgaa tcttccagca gcatcatgtg tccacagttc
     6481 tggatgattt cagtttttgc ttccggaatt gttgaagcga gttccatccc gttacgggcc
     6541 ggagtcattt tgtcatcact tccaattatg cacaacgtgg ggcagcgtat attttttgcc
     6601 gcattcattc cgttttggta aacattgcag gcttccaaat cattgcccaa agctcctttt
     6661 ggtgcagaac tcagcaaagc acgccctccg ccaataagtg acaaaccagg aaccggtgtc
     6721 cttcccagat gtccggctgg tccgtgtccc caggaggtaa ccaaatcata agccagcggg
     6781 tcgtctttgc gggccgcttc aagtaaatcc ggatgcaccg gcatttttgc cgaaggaccc
     6841 atcaaacata atgagcgcac tctttcagga taacgggatg tgcattccaa agccgcaagg
     6901 gctcccatcg agtgtcccac taaggaagca gtatgatacg gttcttccag tcccaaagca
     6961 ttcattaact ctggaatcca gtccgccatt tgttcaatgc tatccagaaa tgaaccgtct
     7021 gaacgtccgt gtcctggaaa atcaaatgcc aggactgaat aaccgtgaaa agcgaaatag
     7081 cgtgtatgta aattccagac tgtgtgatcc attcctcctc catgcatgaa aatgacgagg
     7141 ggaagttccg ggttaagtac tcttcctccg gtagcagcaa aaattgtatt attttggcct
     7201 gaagaactgt tgatcacatt gagcttcatt ttgc
//
LOCUS       DTVD01000168            7209 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3044_length_7209_cov_249.277188, whole genome
            shotgun sequence.
ACCESSION   DTVD01000168 DTVD01000000
VERSION     DTVD01000168.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_7209_cov_249.277188"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            22..897
                     /locus_tag="EYO68_05775"
     CDS             22..897
                     /locus_tag="EYO68_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HIC07350.1"
                     /translation="MIPPPFEYLSPNQLSEAISLLQKHGEDAKILAGGHSLIPAMKLR
                     FALPEYLIDIGGIEGMDYLREEGGQLRIGAMTRESSLEDSELISKKYPLLLDTVKMIA
                     DPQVRNMATVGGNLAHGDPANDHPATMIALRASVIAEGPNGSREIRIDDFFPDFFTTA
                     LETNEILTEIRISSPPPKSGGAYLKIERKVGDYAAAAVACQLNLNTAGSIEQIGIGLT
                     NVGPTPIRATSAENLLRGKSPSDDLIKEAGLLAAGDSDPSDDLRGSAEYKKDLLCELT
                     QRAIRISIERSERSG"
     gene            900..1382
                     /locus_tag="EYO68_05780"
     CDS             900..1382
                     /locus_tag="EYO68_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC07351.1"
                     /translation="MSKHTVTIEINGSSFTREVDSRLLLVHFIREDLQMTGTHIGCDT
                     THCGACTVLMDGRSIKSCTLFTIQANGKSLTTIEGLESGGKLHPLQEGFHEEHGLQCG
                     FCTPGMIMRAVELLGKNPNPTEDEIRTGISGNLCRCTGYVNIVKSVQYAAEKMKAEVA
                     "
     gene            1379..3748
                     /locus_tag="EYO68_05785"
     CDS             1379..3748
                     /locus_tag="EYO68_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-monoxide dehydrogenase large subunit"
                     /protein_id="HIC07352.1"
                     /translation="MKELKTSAEVGGMGHSVKRKEDARFIRGKGNYTDDVVLPGMLHM
                     DIVRSPYAYAKIKSINTDKAMAIPGVHAVITGEVLKGYNLHWMPTLMSDTQMVLPTDT
                     VMYQAQEVAAVIADDRYIAADGVEAVEVEYEPMKAVVDPFKAMEKDAPILRTDKEGKT
                     DNHIWHWETGDAEKTEQVFKNADVVVEEKVYLPRIHVCSIETCGCVASFDPAEGKLTV
                     WMTTQAPHAIRTVFALVAGHVGLSEEKIRIISPDIGGGFGGKVPIYPGYVIAVAASVV
                     IGKPVKWVEDRSENLQADSFARDYHMTVEMAASKEGKMEALRIKTIADHGYTDAAANP
                     SKYPAGMFSICTGSYDIKTAFTEMDAYYTNKPPGGIAYRCSFRVTEAVHAIERIVDVL
                     AKKLDRDPAELRLQNFIQPDEFPYKSALGWEYDSGDYPRALKLAMDKIGYEELRKEQL
                     IKRSKGELMGIGICSFTEIVGAGPSKDFDILGIKMFDSCEVRIHPTGKAIARFGTKSQ
                     GQGHETTYAQILAEELGIPYTDIQIEEGDTDTAPYGLGTYASRSTPTAGAAAAKAAHK
                     IREKSKKIAAHLLEVSEEDLEWEVGKFSVKGSPEQSKTIQEIAFAAYTNHPQGLEAGL
                     EATHYYDPPNLTFPFGSYICVVDIDPKTYAIKIRRFIAIDDCGNIINPMIVEGQIHGG
                     LTQGLAPALFEELIYDEDGNILNGSLMDYLVPTAVETPNWETDQTVTPSPHHPLGAKG
                     VAESPTVGAPPAIANAVIDALSHLGVESMGIPITPFKVWQVLKDKGLAA"
     gene            3802..4800
                     /locus_tag="EYO68_05790"
     CDS             3802..4800
                     /locus_tag="EYO68_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIC07353.1"
                     /translation="MFYFFAPFLITIMTEVFFRDYLKLSKKRVPFATAVVVKHDIPIS
                     GKSGDKAIILNDGSLLGWIGGGCTNSIVIEEGLSALNSGKSKLIVIDPENKSDNGPGE
                     KHFQMTCHSGGSLSVYVEPVFPRPLIVVMGNSPVAISLLKMSSELGYETLWTVTPEKE
                     NETLEVDRIQKTFDLKNINLSSPCFIIVCTQGENDWEALESAMKTSVNYVAFVGSRRK
                     TETLKKELSENGVSPDRLGKMVNPAGLDLKARTPSEIALSILAEIVQLLRDDNTLDNQ
                     VVSESEEKAIDPVCGMKVDTELTKITFQFKSQDYHFCCNGCKTKFKNNPELFLIAS"
     gene            4873..5457
                     /locus_tag="EYO68_05795"
     CDS             4873..5457
                     /locus_tag="EYO68_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07354.1"
                     /translation="MEVKAEKNLKLNQDPDMIWKCMIDPSFMVKSVPGAELTEQLDER
                     NFKGKISIKIGPVTAKFNGEAEFTKLEEADYELTMEGKGLDTSGKGGANMTMNIKLST
                     LEEGGTEMQSSMSLSITGRLAQFGARMIVAVNNKMFDQWATSFTELLNEQTSSKDSEE
                     KSTNQPDNGNVAEPTPVKALPLAWAAIKGLFGSK"
     gene            complement(5596..6231)
                     /locus_tag="EYO68_05800"
     CDS             complement(5596..6231)
                     /locus_tag="EYO68_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIC07355.1"
                     /translation="MALRLGDIAPDFSAETTQGNINFHDWLSDSWGILFSHPKDYTPV
                     CTTELGYMSKIKPEFDKRNVKILAISVDPVDSHNGWVGDINETQNTTVNFPIIADTDR
                     KVSDLYDMIHPNADDTLTVRSVFVIGPDKKVKLTITYPASTGRNFDEVLRVVDSLQLT
                     SDYQVATPVNWKHGEDCIVVSSIPTEDIPEKFPKGHREIKPYLRYTPQPDI"
     gene            complement(6495..7127)
                     /locus_tag="EYO68_05805"
     CDS             complement(6495..7127)
                     /locus_tag="EYO68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC07356.1"
                     /translation="MFDRKKHWGSVYQEKSPSDVSWYQKEPKLSLELIRCTQVASNEA
                     IIDVGGGASVLVDHLCKECFTNLAVLDISENAIASVKKRLGDSAKSIEWFESDITQFD
                     APQKFSLWHDRALFHFLTDPSDRKSYVKALKNALRPEGHLIIATFAIGGPEKCSGLEI
                     VQYDSEKMIAELGDNFELIEERNEVHITPANKEQKFIFFRFLKVPKNRYT"
BASE COUNT         2232 a         1343 c         1550 g         2084 t
ORIGIN      
        1 aattttccca caggaggatt tatgattcca ccaccatttg agtaccttag tcctaatcaa
       61 ttatctgaag caatttccct gctccaaaag catggggagg atgcaaaaat actagctgga
      121 ggtcatagtc tgattccagc gatgaaactc agatttgcat tgccagaata tcttattgat
      181 atcggcggaa tagaaggaat ggattacctt cgtgaagaag gaggtcaact aagaataggt
      241 gccatgactc gagaatcatc cctagaagat tctgagttaa ttagcaaaaa atatcctttg
      301 cttttggata cggtaaagat gattgctgat ccacaggtaa gaaatatggc tactgttgga
      361 ggcaatcttg ctcatggtga tcctgcgaac gatcatcctg caacaatgat tgcgttaaga
      421 gcctctgtca ttgcagaagg accaaatggc agtcgtgaga taagaattga tgattttttc
      481 cctgactttt ttacaacggc tttggagaca aatgaaattc taactgaaat caggatttct
      541 tcgccacctc ccaagagtgg gggggcttac cttaaaatag aacgaaaagt tggagattat
      601 gctgctgctg ctgtagcttg ccagcttaat cttaatactg ctggttcgat agaacagatt
      661 ggtattggtc tgacaaacgt aggacctact cctattcgtg caacctctgc agaaaactta
      721 ctcagaggta aaagcccgag tgatgattta attaaagaag caggactttt ggctgccggc
      781 gattctgatc catcagatga tctccgaggt agtgcagaat acaaaaagga tctcttatgt
      841 gaactaacac aaagagcaat aaggatttca attgaacgtt ctgaaaggag tggataatca
      901 tgtcaaaaca cactgtcacc attgaaatta atggttcaag ctttacccga gaagtggatt
      961 cacggttgtt acttgttcat tttattcgtg aagatttaca gatgacagga actcacattg
     1021 gctgtgatac cacacactgt ggtgcatgta ctgtgctcat ggacggacgt tccataaagt
     1081 catgtacact atttacaata caggctaatg gcaaatcatt aactactatt gaaggtttgg
     1141 aaagtggagg gaaacttcat ccgcttcaag agggtttcca cgaggagcat ggtctccagt
     1201 gtggcttctg tactccaggt atgattatga gagcagttga acttttggga aagaacccaa
     1261 atccaaccga agacgaaatc cgtactggta tttcagggaa tctttgccgt tgtaccggat
     1321 acgttaatat tgtgaaatct gtccaatacg cagcagaaaa aatgaaagcg gaggttgcat
     1381 gaaagaatta aaaacaagtg ctgaagtcgg aggaatgggg cacagtgtta aacgtaaaga
     1441 agatgcccgt tttattaggg ggaaagggaa ctacactgat gatgtagtac ttcctggaat
     1501 gctgcacatg gacattgtca gaagcccgta tgcctatgca aaaatcaaaa gtatcaatac
     1561 agacaaagca atggctattc caggtgttca cgcagttata acaggtgaag tattgaaggg
     1621 atacaacctt cactggatgc caacgctcat gtcagacact caaatggttc tcccaactga
     1681 cacagtaatg taccaggcac aggaagtcgc agctgtgata gctgatgaca ggtacattgc
     1741 ggcagatgga gttgaagctg ttgaagtaga gtatgaaccg atgaaggcgg ttgtagatcc
     1801 atttaaggct atggaaaaag atgctccaat tcttcgaaca gataaggaag gtaagactga
     1861 caatcatatt tggcactggg aaactggtga tgcagaaaaa accgaacaag tttttaagaa
     1921 tgctgatgtg gttgttgaag aaaaagttta tctgcctaga atccacgtct gctcaattga
     1981 gacttgtggc tgtgttgcct cctttgatcc tgccgaggga aaacttactg tctggatgac
     2041 aactcaggca cctcatgcta ttcgaaccgt ttttgcatta gttgctgggc atgttggact
     2101 ttccgaagag aaaattcgta ttatttctcc tgacattggt ggaggttttg gtggtaaagt
     2161 cccgatatat ccaggttatg tgattgccgt tgcagcgtca gtggtaatcg gtaagcctgt
     2221 caagtgggtt gaggatcggt cagagaatct acaggccgat tcttttgcaa gggattatca
     2281 tatgactgtg gaaatggctg caagtaaaga aggtaagatg gaagcattga ggatcaaaac
     2341 aattgctgat catggttaca cagatgcagc ggcaaatcct tcgaagtacc ctgcaggtat
     2401 gttttcaatc tgtacaggct cctatgacat caaaacagcg tttaccgaga tggacgcata
     2461 ctataccaat aaaccgcctg gcggtatcgc ctacagatgt tcgtttagag ttacagaggc
     2521 agtacatgca attgagcgaa tagttgatgt tttagctaaa aaacttgaca gagatcctgc
     2581 agaattacgt ttgcagaact tcattcaacc agatgaattt ccttataagt ctgctttagg
     2641 ctgggaatat gacagcggag attatccgag agctctgaaa ctggctatgg ataagatagg
     2701 ctatgaagaa cttcgtaagg aacaattaat aaaaagatcc aaaggtgaac tgatgggcat
     2761 aggtatctgc agtttcacag aaattgttgg tgcaggtccc tctaaagatt ttgatattct
     2821 aggcatcaaa atgttcgaca gttgtgaagt ccgcatccat ccaaccggta aggctatagc
     2881 gagatttggt acaaaatcgc aagggcaggg tcacgagaca acctatgccc aaattcttgc
     2941 tgaggaactt ggtataccct acacagacat acagatagaa gagggagaca ctgacactgc
     3001 tccatatggt cttggaactt atgcaagccg aagtactcca acagccgggg ctgctgctgc
     3061 caaggctgca cacaagatac gtgagaaatc taaaaaaatc gcagctcacc ttcttgaggt
     3121 aagtgaagaa gacttggagt gggaagttgg caaattttcc gtaaaaggct ctccagaaca
     3181 gtccaaaact attcaggaaa ttgcattcgc tgcctataca aatcatccgc aaggactgga
     3241 ggctggacta gaagcaaccc attattatga tccgccaaat cttactttcc cttttggttc
     3301 atacatctgt gttgtagata tagatccaaa aacatatgca ataaagatca gacgtttcat
     3361 tgcgatagat gactgcggaa atattataaa tccgatgatt gttgagggtc agatacatgg
     3421 ggggctaacc caaggcctag cacccgcatt gtttgaagaa ttgatctatg atgaggatgg
     3481 taacatccta aatggttcac ttatggacta cttggttcca actgctgttg aaacccctaa
     3541 ttgggaaaca gatcagactg taactccttc tcctcatcat cctcttgggg ctaaaggtgt
     3601 cgctgagtct ccaacagtgg gggcacctcc tgcaattgct aatgcagtga ttgacgcctt
     3661 gtcgcacctt ggtgttgaaa gcatgggcat ccctatcacc ccgtttaagg tttggcaagt
     3721 acttaaggac aaaggactag ctgcctgata aaatatattc ccggaacaaa aaaaatagtt
     3781 ccgggaagcc tcccatcccc tttgttttat ttttttgctc cttttttgat aactatcatg
     3841 accgaggttt tttttcgaga ttatctgaaa ttgagtaaaa aaagggtccc ctttgcaaca
     3901 gcagttgttg ttaaacatga tatccccatt tctggaaaat ctggagataa agcaatcata
     3961 ctaaatgatg gtagtttact tggatggatt ggtggaggct gtactaactc cattgttatc
     4021 gaagagggtc tgtctgcctt gaattctggg aaatcaaagt tgatagtaat tgatccagag
     4081 aacaagtcag ataatggtcc tggtgaaaaa cattttcaaa tgacatgtca tagtggagga
     4141 tcattgagtg tatatgtaga accagttttt ccaagacctc ttatcgttgt tatgggaaat
     4201 tcgcctgttg ctattagtct tctaaaaatg tcttcagaac tcggttatga aaccctatgg
     4261 actgtcaccc ctgaaaaaga aaatgaaaca ctggaagttg atagaataca gaaaactttt
     4321 gatcttaaaa atataaatct ctcttcccca tgctttataa ttgtttgcac gcaaggagaa
     4381 aatgattggg aggcacttga atctgcaatg aagacctcag ttaattatgt tgcttttgtt
     4441 ggaagccgta gaaaaacaga aactcttaaa aaagaacttt cggaaaatgg tgtaagtcca
     4501 gacagattgg gaaaaatggt caatcctgca gggttagacc ttaaagcaag aactccttca
     4561 gaaatagcct tgagtattct tgcagaaatt gttcaacttc tcagagatga caacacccta
     4621 gacaatcaag tagtttcaga atctgaagaa aaggccatag atccagtttg tggaatgaaa
     4681 gtggacacag aattaaccaa aattactttt caattcaaaa gtcaggatta tcatttttgc
     4741 tgtaatggat gcaaaactaa attcaaaaat aatcctgaat tatttcttat cgccagctga
     4801 aatttaaaac tgtatcagat ggtcttattt tcattataat ttataaattt tcaaattaat
     4861 taacatataa atatggaagt caaagcagag aaaaatctaa agttaaatca agatccagac
     4921 atgatttgga aatgtatgat tgatccatca ttcatggtta aatctgttcc tggtgcagaa
     4981 ctaacagagc agttagatga aagaaatttt aaaggtaaga tcagtattaa aattggtcct
     5041 gtaaccgcaa agtttaacgg ggaagcagaa tttaccaaac ttgaagaggc agattatgag
     5101 ttgaccatgg aaggcaaggg cctggatacc tcaggtaaag gtggagctaa tatgactatg
     5161 aatataaagc tgtccacttt agaggagggt ggaactgaaa tgcaaagtag catgtcactt
     5221 tctataaccg gtagactggc acaatttggt gctagaatga ttgtagcagt aaataacaaa
     5281 atgtttgacc aatgggctac gagttttaca gagctgctga atgaacaaac atcatcaaaa
     5341 gattcagaag aaaaatcaac caaccaacca gataatggta atgtagcgga accaactccg
     5401 gtcaaagcac ttcctctggc ttgggcagca atcaagggac ttttcggatc taaatagtaa
     5461 aaaataatta tctggattta acaccaactg tttcagttac aattatgccc atgtggtaat
     5521 tttgatcata ttatctttaa ccagaatttt tccttgattc aacagggacg gccctgttta
     5581 gaaccgtcat gttttttata tatcaggctg cggagtgtat ctgaggtagg gcttgatttc
     5641 tctatgtcct ttggggaact tttccggaat gtcctctgtt ggaatggaag aaacaacgat
     5701 gcagtcctct ccgtgtttcc agtttactgg tgttgctact tggtaatctg aggtgagctg
     5761 cagcgagtcg acaactcgca gtacttcatc gaagttgcga ccagttgatg ctggataagt
     5821 aatagttagc ttgaccttct tatctggtcc aataacaaac accgaccgaa ctgttagagt
     5881 gtcatctgcg tttggatgaa tcatatcgta aagatccgaa acctttcggt ctgtgtcagc
     5941 tatgattggg aagttgactg ttgtgttttg tgtctcatta atatctccaa cccatccgtt
     6001 atgagaatca actggatcaa cgcttatagc aagtatcttc acattgcgtt tatcaaattc
     6061 tggctttatt tttgacatgt agcctagttc agttgtacaa actggagtgt aatccttagg
     6121 atgggaaaag aggatgcccc aagaatcact aagccagtca tggaaattta tatttccttg
     6181 cgttgtttct gctgagaagt caggtgcaat atctccaagt cttaatgcca ttttgtctcc
     6241 taaataaatt ttaagtattg cgtaaccatt tagagctgag atctttttta tttaaaaaga
     6301 acattttaaa aataatttat cccatccttt cttagtggaa tataaatgaa atattttaat
     6361 tttacaaatt aataatcgtg tcaatttatg aattcataaa attgtcatta gatagcattg
     6421 taatcgcaac gaataaatgt tgaagtgtga ggccttgagt tagtggagct ttttcaataa
     6481 gacacatcca atagttatgt atatctgttc tttggaacct taaggaaacg aaaaaatatg
     6541 aacttctgtt ctttgttggc tggagtgata tgcacttcgt ttctttcctc tatcaactca
     6601 aaattatcac ctagttcggc tatcattttc tcagagtcat attgcacaat ttctagacca
     6661 ctgcattttt cagggcctcc aattgcaaaa gtcgctatga tgagatggcc ttcaggcctt
     6721 agagcatttt ttaatgcctt tacgtaactt ttcctatcgg atggatctgt taggaaatga
     6781 aagagagctc tatcgtgcca aagtgaaaac ttttgtggtg catcaaattg agtaatatca
     6841 gattcaaacc actcaatgct tttcgctgaa tcaccaagcc tctttttgac actagctatt
     6901 gcattttctg aaatatctaa aactgccaaa ttcgtgaagc attctttaca taggtgatct
     6961 actagaacag aagcaccacc cccaacatca atgattgcct cattactggc aacctgagta
     7021 caacggataa gctccaggga tagctttggt tctttttggt accagcttac atctgaggga
     7081 gacttctctt gatacacact cccccaatgc ttttttctgt caaacatcac aaagtactat
     7141 gtcacaaact attttgatat tatttttcat tcgttctgtt cagaactact aataagaaaa
     7201 tgatgtttt
//
LOCUS       DTVD01000169           24169 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_307_length_24169_cov_395.056191, whole genome
            shotgun sequence.
ACCESSION   DTVD01000169 DTVD01000000
VERSION     DTVD01000169.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 24169)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24169)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24169
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_24169_cov_395.056191"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="EYO68_05810"
     CDS             complement(<1..956)
                     /locus_tag="EYO68_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIC07357.1"
                     /translation="MTKMSPLLERALETLQIEIAGLEGLIKRLDSEFEEAVNLLFACG
                     GKVILAGIGKSGLIAQKIAATLTSTGTPALFLHAGESTHGDLGIITKQDVVIAFSYSG
                     ETNEVLQMIGHIQAIGVPLIAMTGKPDSTLAQAATVHLSIAVEKEACPLGLTPTASST
                     ATLALGDALAMCLLEQRQFSEDDFALFHPGGSLGRKLTIKIEDVMVSGEPLPVVPENM
                     PMREALNVLSRKNLGIVVAVNRDEKISGVFTTGDLMRLLEDEQSFLDKPLLEFAHPDP
                     KTIAPEELAAKALHVMETHSITCLVVADQENRPIGIVQIYYIL"
     gene            complement(953..2083)
                     /locus_tag="EYO68_05815"
     CDS             complement(953..2083)
                     /locus_tag="EYO68_05815"
                     /inference="COORDINATES: protein motif:HMM:PF00132.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HIC07358.1"
                     /translation="MSTFAENLKTIPTVENTVILRLYNEREELVSIIPNIPGRQGSIR
                     VFQHLADAKGQINFEAAKEGLRLFGEHVEDAQKNPGKHQKLELLLGLKNREALRIEIV
                     ESTCSNLLNNLYDQKASAEECEVFIELLNQGKLRAAEKVNNFWVPQPYIIDGVLNYFG
                     THGNARMEGKYWDKIPLKTANYTDQEFEQGGVRYAPGCMVRTGAYIGPQTVIMNLAFV
                     NIGAYISGEGCMIDGGARVASCAQIGKNVKFGAGSGIEGILEPAGRLASIVEDHVKIG
                     AMCEVTGIIGEGSVIASGVIMASGKKIYDEDTGDFVSPLECIVGDKKFLLPVIPPYRL
                     AVGGSLPSKKGNHNTDAVILKAGDLRDSATMRHFTKQGILYG"
     gene            complement(2185..3555)
                     /locus_tag="EYO68_05820"
     CDS             complement(2185..3555)
                     /locus_tag="EYO68_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIC07359.1"
                     /translation="MSNILCIGAGYVGGPTMAMIAKNCPEHKITVVDINQDRIARWNS
                     DELPIYEPGLLKVVKEARGRNLFFQTDVAAATTDADIIFVSVNTPTKMFGEGAGKAAD
                     LQFWEKTAHEILANCRKPEVVVVEKSTLPVRTAEAMAKILESGKSSTKFSVVSNPEFL
                     AEGTAIEDLTNPDRVLIGGEQNEHGRKAAETVAALYAHWVPQERILLTSVWSSELSKL
                     VANAMLAQRISSINSISALCERTEADIAEVSKAIGMDTRIGSKFLEASIGFGGSCFRK
                     DILHLSYLCEYYGLPEVADYWESVVRINEWQTDRFFQSILQEQFNTLAGKTITLLGFA
                     FKQDTGDTRDSPAIPLCEKLLAEHACVRVHDPKALENARQDLKEIQGDISFVEDVYEA
                     ADCAHALALVTQWKEYRELDFERIFQAMAKPAFIFDGRNHLDHKKLYEIGFNVFPLGK
                     MPLKHL"
     gene            complement(3597..5237)
                     /locus_tag="EYO68_05825"
     CDS             complement(3597..5237)
                     /locus_tag="EYO68_05825"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIC07360.1"
                     /translation="MLFLKNLQQYFSGRGPLIHVVLDGWGVGAADETNAVNRANLPVM
                     SRLIRGCPYTQLWTHGKYVGLPNEKDMGGSEVGHMTMGAGMVMEQGPTLIQNLLQSGE
                     FFENPVLSRIIQNCVERDTPLHLLGLLSNGNIHSHVDHTEAIIRHAFQSGIRRCYLHA
                     LLDGRDVGVQSALDFTEPFEKLFSELKGQRPGIDYAFASGGGREVITMDRDNNWEKIE
                     TGWQIHVQGQSENQFPSIRDAIEHFRKQNPEIIDQDIPGFVIIRNGEAVGRIEDQHAL
                     IFTNFRGDRATEFSQAVLADDFPYFERYRCPEVLFAGMTQYDQDNQIPPDYLVGTPVV
                     EEPFGKRILELGLKQFRLSETQKFAHVTFFYNGGYREPLDPLQENYHFIASDKIPSFA
                     ERPAMKAPGISKKAVEFINSGEYQYGLINFANADMVGHTGDFQATVRAVEAADAALDN
                     IVRAIDAVNGLLVITADHGNADEMLISNQNGTLEISTKHSLNPVPFLIYDPLYNGDYR
                     LKPFGQDYNNNLSNIAATNFLLLGQAVPDDLAPSLFAD"
     gene            complement(5258..5983)
                     /locus_tag="EYO68_05830"
     CDS             complement(5258..5983)
                     /locus_tag="EYO68_05830"
                     /inference="COORDINATES: protein motif:HMM:PF00994.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIC07361.1"
                     /translation="METAGIIIIGNEILSGKVTDINSPYLCKELRILGVEVQQIITIP
                     DIPEIIGRTVRDFSEQFSWVFTSGGIGPTHDDITVASIAKGFGIELFEAPQIMKAIQN
                     YHGDKMTAAHRRMAMIPEGSELIEYAEGRGPQLKFRNIFVFPGIPEYLKIRFASIKER
                     FRTTPIVLKQIYLKADEGEIADSLDATMSEFPQLMLGSYPKVSGSDYDVKLTLECRDS
                     EYLDKALNFLCERLPEECILRFE"
     gene            complement(6006..6341)
                     /locus_tag="EYO68_05835"
     CDS             complement(6006..6341)
                     /locus_tag="EYO68_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parvulin peptidyl-prolyl isomerase"
                     /protein_id="HIC07362.1"
                     /translation="MFRRVLVELVTASHILITYEGALRATKKRDRKEARILAEQILKD
                     LKRGKDFAELAREHSDGPSGPKGGDLGRFTRGQMVPEFDQAVFNLKPGEVSGVVETQF
                     GYHIIKRIK"
     gene            complement(6780..7382)
                     /locus_tag="EYO68_05840"
                     /pseudo
     CDS             complement(6780..7382)
                     /locus_tag="EYO68_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010645783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(7556..8143)
                     /locus_tag="EYO68_05845"
     CDS             complement(7556..8143)
                     /locus_tag="EYO68_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HIC07363.1"
                     /translation="MSKSLFNNLRVVLLDRDGVINEEPGPILTPEQFVMIPKSATAVA
                     RLNAEGFRCFVITNQAAFARGDLSESAFQAITDKMQHELQKAGAHLDGQYYCPHHPDW
                     ENGERREVPAPCACRKPGTLLLEQAAEEHGFSAEESILIGDSTSDFAAASKWGICSIG
                     VHTGHGGQDSKAKADPDYWRDDLWSAVEFLLANAS"
     gene            complement(8153..9292)
                     /locus_tag="EYO68_05850"
     CDS             complement(8153..9292)
                     /locus_tag="EYO68_05850"
                     /inference="COORDINATES: protein motif:HMM:PF10023.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIC07364.1"
                     /translation="MFTILELTFPKQIIAVRSGYLLLLALGSVIFLSGCSDLGFYWQA
                     ASGHLDLLNRKQDIRELLDSPDTSPKLKRKLKLVESVRAFAVSQMALPENEGYTGYVD
                     LGRSYVTMVVTAAPELELKAHKWCYWFVGCQEYRGYFDEGDARLYAEEMEQQQLDVSV
                     GPVTAYSTLGWLNKPWLPNYFSDPVLSTFIHRRDAELIAVLIHEMAHQVVFVNNDTSF
                     NESFAVFVEQEGLRQFLIQSSNSDFFDGDGKEIYQWYLNARKDRKLFQQLIKTTYDKM
                     KILFASELSVAEKLRNKQVLYDQLRENYEIDKKAFKVLSYDNWFAKNLNNTHLLGVQR
                     YQSRVEQFTHLFEKQGRKWSQFFEAVRELAKVSDKERKQQLELLD"
     gene            complement(9271..11247)
                     /locus_tag="EYO68_05855"
     CDS             complement(9271..11247)
                     /locus_tag="EYO68_05855"
                     /inference="COORDINATES: protein
                     motif:HMM:PF11412.6,HMM:PF13098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIC07365.1"
                     /translation="MPPRSLFKSSIYRALRQLLLLLCLSLLFTSGFPHTTIAQFDDDV
                     VIPESVVTFQVRLEPEKPRPGEHARIIVDLQIHPESGWHVFSVIPGEGDFAPIPTSLT
                     FEADPLILIGPVYESNPIIAADPVLDMVLSYHLMKATLFQNILIPDDISGGTVLNKGG
                     KLRYQACSERICLPPKTELFSLQIPLGTGTVRTEYKIPQFAVDSPPEEVSEIEDALKE
                     GFWGFIGLAVIAGLLALLTPCVFPMIPVTVAFFTKQGEAGSKETLRLAALFGAGIVGT
                     YTVTGMLLSVVLGAAGAVQFATNGWVNLAIGVMFTLFAFSLMGFIQLNAPGGFGNLAD
                     RWSRKLSGTAGVLAMGLAFTLTSFTCTVQFVGTLLIAASQGMWFWPIVGMLVFSTVFA
                     FPFFLLGLFPRLIQTMRSQSGNWMEHLKIVLGLLELAAAFKFFSNTDLVWQWGVLDKD
                     FVLAAWVVLCVIGALFLLGAVKIHHVRVEKTGGTGFTAAFLFAFLGIYLLRGLFGVPL
                     NPWVDTYLPPVLRTLSESRQGFATSSGTSGEAVSHSLPWQNDLADALIQAKAENKRVF
                     IDFTGYTCVNCRWMEKNIFSHPDVMARFQQEFVLVQLYTDGGDKAAANQQMQISRFQT
                     VALPLYVILDSDNNLLAKHAGILNPAKEFLQFLN"
     gene            complement(11378..11704)
                     /locus_tag="EYO68_05860"
     CDS             complement(11378..11704)
                     /locus_tag="EYO68_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07366.1"
                     /translation="MNLVKLLEEGNYFEIQKRSGRSKTLEKDSTPFCGALRPHYNPKM
                     LLLLSSPLESRSDVFEFRSEDILYAEELSSLTKPNGVTVERVRLWVRNGSPAMRMEPL
                     RVGSAE"
     gene            complement(11723..12766)
                     /gene="ilvC"
                     /locus_tag="EYO68_05865"
     CDS             complement(11723..12766)
                     /gene="ilvC"
                     /locus_tag="EYO68_05865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIC07367.1"
                     /translation="MKDIEINGIKETIVERSDYPIEECKNILGNEVTAILGYGPQGRG
                     QGLNMRDQGFKVVLGLRKGRSWDKALADGWLEGETLFETEEAAQRGTILQYLLSDAGQ
                     IAAWPVVKASLNEGDALYFSHGFGIVFHQDTSIIPPENVDVILVAPKGSGLTVRTHFQ
                     EGRGINASFAIHQDYTGRARERCISTAFAIGSGHLFETTFENEVHSDLTGERCVLMGL
                     LQGAFLAQYEVLREHGHSPSEAYNETIEEALQSLYPLVAEKGMDWMYANCSTTAQRGA
                     LDWAPRFRDVIKPVVEKCYQSVLDGTEAKISINSNSQNDYREKLEKELEEVSSQEMWQ
                     AGKVLRKLRPENL"
     gene            complement(12913..15483)
                     /gene="xdhB"
                     /locus_tag="EYO68_05870"
     CDS             complement(12913..15483)
                     /gene="xdhB"
                     /locus_tag="EYO68_05870"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HIC07368.1"
                     /translation="MKENGNQVTNVSAASSVTNDDLFVDDLPSAQNELRSDFIESPAA
                     QKRDRMKVVGKQVAHDSAVGHVTGDALFVDDLPFTKNELVVDFIGSPVAHGEIINLNS
                     EELAQLDGITGVFTHADIPGHNLYGPVIQDERFLAEGVVEYVGQPIAIIAGESREAIR
                     QAKKKLRLEIKELVPVFTIEEAISAKQFIGTTRRFKQGNFEKAWSEAEHTLKGTFICN
                     GQEQFYLESQAALAWPGEHGEIQIHSSSQNPTEIQEVIAEALGLGFNEVVCVCKRMGG
                     AFGGKETQAVIPAVMVALVVAKTKRPARIAYTKDEDMRSTGKRHPYKIHYKVAFTADG
                     KITGVKFDIFSNGGAGADLSTAIMERTLFHSENAYFIPNLIFNGTICKTNFPPNTAFR
                     GFGGPQGMANIENVIQEIAIFLKKDALEIRRLNCYSHDERNVTPYGQIVRNHLLPEII
                     DQLVETSGYRQRLTEVEDFNRQSETHLRGLALTPMKFGISFTTKFLNQGNALVNIYKD
                     GTVQVSTGGTEMGQGLNTKIRQLVADEFSISYDDVRMMITSTEKNNNTPPTAASAGTD
                     LNGFAAVNACRKIRKNLTKFASSYFAAKQGLDPAPEHICFEAGKSGGNSSVFDDRNPE
                     NRLTFRELVKLAFMDRVSLGERGFYSTPEINFNRDTEKGEPFFYYTTGAAVSEVLIDR
                     LTGHLKLERSDLLLDIGESINPGIDRGQIIGGFIQGVGWVTNEDLRYSEKGELLTYSP
                     TTYKIPNIQDLPEIFNVDTIHNPHHQINIRRSKAVGEPPLMLCLSVWIAVKHALSCVR
                     NDVCPRLNLPATGEEILLRLTELNDYSKEHRTIDFSALESEVSLVVLPSSSAETKATV
                     "
     gene            complement(15480..16946)
                     /locus_tag="EYO68_05875"
     CDS             complement(15480..16946)
                     /locus_tag="EYO68_05875"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01799.18,HMM:PF03450.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07369.1"
                     /translation="MRDYLLIYVNGRRYKISGKRGFQSLSDFLRYDLGMVGTKVVCAE
                     GDCGSCTVLIGRVRNSQLLYEGIDSCIQYLYQLDCSHVITVEGLKDEAQIIHPVQKAM
                     VDAAGSQCGYCTPGFVMALAAMLETGETLTRHSVQDGLTGNLCRCTGYEQIIDAGLSL
                     NQKVIPKASKCYDAKAILADFMEHVVQPVYCEYEKKWNGAGQQVQFFVPAYLEEALEF
                     KQKNKHVTVVAGGTDISVQMNKERLEPNCIMSLTHLTGLEILEIENKTVTVGAKVTWT
                     ELGEFCQEHLPELDEIISIFASRQIKNAATLAGNIANASPIADSLPFLHVIDAEMELT
                     ASHGTRWVNINRFYHGYKNTEMKPDELITRIRWNLPEPETVLKLYKVSKRKDLDISTF
                     TAGILLRLKKGKIQNARLAFGGVGPVVLRLPKTEEFLQGKPMLPGIFHEAGKLARDEI
                     SPISDVRASSDYRLQLAENILLKFFHECSDNQNKKEAA"
     gene            complement(16939..18582)
                     /locus_tag="EYO68_05880"
     CDS             complement(16939..18582)
                     /locus_tag="EYO68_05880"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07370.1"
                     /translation="MNSSEIEKSHSEQRLPLQLSRITKSFPGIVANDSVDFTLEEGEI
                     HTILGENGAGKSTLMNMIAGLYPADAGEMRVFGEQVNFTNPRQAIEKSIGMVFQHFML
                     VRNHTVAENIILGLPGVGKLKLKEVHQQIREISERYDLYVDPEKKIQELSVGEQQRVE
                     IVKVLFRGARILILDEPTAVLTPQESEALYEIMEKMVNEKHSIIFITHKMKEVMALSH
                     RITVLSRGKVMATLKKDETNPQELADLMISNLEAKDLVKVSEFLRDTTVEEKDGDGSV
                     SQKQKKKFGNLTVALKDIHAVNDLGYPALKGISLDIRAGEILGMAGISGNGQPELTDV
                     LSGLRSPTRGKYQFEDVPLNKPTVYEMIDMGVGYIPEDRKRFGVSSGMSIAYNFLLRD
                     FQNPQFCSRQLLKLSNIFDYGQKKMQEFDIRAPTEKTPVGLLSGGNMQKVILAREISR
                     PLKLLLAAQPTRGLDIHATSFIREQICQARDRGLAVLWISEDLDELLMVADRIAVIFD
                     GRIVGTLSHEEASVTLIGQMMTGMNKNGTEHVMEKREYA"
     gene            complement(18876..20486)
                     /locus_tag="EYO68_05885"
     CDS             complement(18876..20486)
                     /locus_tag="EYO68_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIC07371.1"
                     /translation="MKIRQNPSSLNTLRHASNHFNKVKGGIERLSSGVKINRGADGPA
                     SLISSERLRGHIVGLQQVYNNASSSVSMLQTAEGALNEVSDLLIKIKQLTVHAQNEAI
                     NSSDMLSADQQEIDNLLSSIDRISENTEFGGKRLLDGSMGVNGTAVGNYLRFVSAEAT
                     ASTSPEQGWKVDIQQVATRAKKTGSIAININNIRNGLQIVLNEGGRNLTLNTKDGRLG
                     QEIEQLIKNADQDRLLFPQAEISSQIRELIIFALSQAAEESGLDLYIAETPDKKLLVR
                     HNKFGDSASFSVTSSVAGILSDDANIAQKSESGINIEGTINDEVTLGEGQLLTTLEGM
                     EAEGITIKYDREIGLINTPILNESGELIGTELIEEKNEDIVGGPNNPKIEGYVHVSQM
                     SKEFQVGLDKKSNPTFSFANVRTSLMGNGIHNKSGFSSLAEIEVTSSQGAQDASIIVD
                     RVIEEISVQRGKLGSFQKNALENNMNSLKIAEENITQAESTLRDSDMAAEMSNLTKDQ
                     ILLQASTAMIAQANQVPETVLGLIQNRG"
     gene            complement(20630..21220)
                     /locus_tag="EYO68_05890"
     CDS             complement(20630..21220)
                     /locus_tag="EYO68_05890"
                     /inference="COORDINATES: protein motif:HMM:PF01728.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIC07372.1"
                     /translation="MKKIKDHYFHKAKRNGYVARSAYKLEEIDKKHRIIRKGNLVLDL
                     GCSPGSWLQYASRKVGEEGEVLGVDLQPVNHSLPAHVKVLQADIFELTAKDLEMSGGM
                     VDVILSDMAPKTTGIRATDAQRSYALNQQVLQLAEELLRPQGMLLVKAFQGAPLDELR
                     NAFRNSFAKVKLCKPKSSRAESVEIFLLGMGKKQNN"
     gene            complement(21217..22608)
                     /gene="radC"
                     /locus_tag="EYO68_05895"
     CDS             complement(21217..22608)
                     /gene="radC"
                     /locus_tag="EYO68_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HIC07373.1"
                     /translation="MAEHEHFFQILQKKLGASLRMHPWTAAQLNSSNIRLLSRKNLGE
                     KLLDRILPLFEVSEELTRFAGLQPLYDGINLLDPVYCRKDEVLRMLEKCTGLNDSQRE
                     QLTSAVMVFMDIVKKTDLNPMQLKSIKTLSLWWKIYPDLKPWNALKWLWQEGIAVPHS
                     QSGYRAWRRFSHGSNSESAKNASLHPKKWLEICEEQNVFETAFEADRLSAAFSGEGSH
                     AGLAGVCGNLPDCDNCELSLECHWYAAEGNSEKMAIEEKIQRNKISTADIPELMQWLL
                     SSNPEEAKALQNSLNAEAPLKDWSRERLRELENQQPLDSNLILRLEALREMCRNYGIE
                     KLKPQDQFNSSREIFNHFHQQLERQKQEQFIIVLLDNKHRYLAEEDVTKGILNKSLVH
                     PREVFASAIEHRAAALICVHNHPSGDPEPSQEDFRITERLVEVGKLVGIPVLDHVIVG
                     GDNYTSFADKGLL"
     gene            23060..24028
                     /locus_tag="EYO68_05900"
     CDS             23060..24028
                     /locus_tag="EYO68_05900"
                     /inference="COORDINATES: protein motif:HMM:PF01618.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07374.1"
                     /translation="MLDKIGTLLGMLMGVSLVIFGIIWPDHLSNYYMYQFREFELSLE
                     ALKVSQAPIEEIQALKASFKMFQESWFGSISRFADLKSLLIVLGGSYAATLIAFRFGD
                     AMRAILFIAKAFLTGKADKDFLEVYHTVISLCEKRANKELITDEEISAVKNKDLQNWL
                     QDFIAVDLVTEEMIEEIVRSEIEMYNYRSFEEIDMLEFMGRAAPAFGMIGTVVGLIMM
                     LGSVGGEGGADIAGLMGGMAVAMITTLYGVLLSQLIFLPIASKRFQLKETQVLLMEMI
                     REGLLYLKRRELPETAAKDLVIYLPPKLRKKVMKEEQAAMGQGLGL"
     gene            24030..>24169
                     /locus_tag="EYO68_05905"
     CDS             24030..>24169
                     /locus_tag="EYO68_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07375.1"
                     /translation="MSRSASSEGGWITTFADLMTLLFCFFVLLNALSTQPKNCGGLEQ
                     FL"
BASE COUNT         6665 a         5666 c         4629 g         7209 t
ORIGIN      
        1 agaatgtagt agatctggac aataccgatt ggacgatttt cctggtcggc aacaacgaga
       61 caggtgatgg agtgtgtttc cattacatgc aaagcttttg ctgcaagttc ttccggagca
      121 atcgttttag gatccggatg ggcaaattca agcaggggtt tgtcaagaaa agactgctca
      181 tcctcaagca gccgcattaa atctcctgtc gtaaaaacac cgcttatttt ttcatcccgg
      241 tttacagcaa ccactattcc aaggtttttc ctggagagaa cattcagtgc ttcacgcatt
      301 ggcatatttt caggcactac aggcaatggt tcccctgaca ccatcacatc ctctatcttg
      361 atggtcaatt tacgaccgag actaccacca ggatgaaaca aggcaaaatc atcttcacta
      421 aactgacgct gctcaagtaa acacatggca agtgcgtcac ccaaagccag agttgctgta
      481 ctgcttgctg tgggagtcag tcccagcgga caggcttcct tttcaacagc aatgctcaaa
      541 tgaactgtag ccgcctgtgc cagggtcgaa tccggttttc cggtcattgc aatcagcggg
      601 actccaattg cttgaatatg accaatcatc tgcagaactt cattggtctc accgctgtat
      661 gagaaagcaa ttactacatc ctgtttggta atgattccca aatcaccgtg agtactttct
      721 cctgcatgta aaaaaagcgc tggtgttcct gtactggtaa gcgtcgcagc aattttttga
      781 gcaatcaaac cacttttacc aattccggcc aggatcactt ttcccccgca ggcaaaaagc
      841 aaatttactg cttcctcaaa ttcagaatcg agtcgtttga tcagcccttc cagacccgca
      901 atttcaattt gtagcgtctc gagtgcacgc tctagcaatg gggacatttt tgtcatccgt
      961 ataaaatacc ctgcttcgta aaatgccgca tcgttgcaga atccctcaaa tcacctgcct
     1021 taagaataac tgcatctgtg ttgtggttcc cttttttact tggcagtgaa cctccaactg
     1081 caagccgata cggtggtata accggcagca aaaacttttt gtcacctaca atacactcta
     1141 agggagatac aaaatcgccc gtatcttcat catatatttt cttaccggat gccattatta
     1201 ctccgctggc aataactgaa ccttcaccaa taattccagt tacctcacac atcgcaccaa
     1261 tcttaacgtg atcttcaaca attgaagcta acctgcctgc cggttcaaga attccttcaa
     1321 ttccggatcc tgcgccaaac tttacgttct taccaatttg agcacaggaa gcaactcgtg
     1381 cacctccgtc aatcatgcag ccttctccgg aaatatatgc tccgatgttg acaaaagcca
     1441 ggttcatgat aactgtctgg gggccaatgt aagcacccgt gcgtaccatg caaccgggtg
     1501 cataacgaac tccgccctgt tcaaattcct gatctgtgta gtttgcagtt ttcagtggaa
     1561 ttttatccca gtatttaccc tccatacggg cattgccatg agtaccgaaa tagtttaaaa
     1621 caccatcaat aatatatggt tggggtaccc aaaaattatt aactttttct gccgcccgta
     1681 atttaccctg attcaataat tcaataaaaa cctcgcattc ttctgcagaa gctttttgat
     1741 catacaggtt gttcaacagg tttgaacagg tactttccac aatttctatc ctgagtgcct
     1801 ctctgttttt caatcccaac agcaattcaa gtttctgatg ttttcccgga tttttctgtg
     1861 cgtcttcaac atgttcgcca aaaagcctca gtccctcttt tgctgcttca aagttaattt
     1921 gaccctttgc gtctgcaaga tgctgaaaga cccggatcga accctgtcga ccgggaatat
     1981 taggaatgat cgaaaccagt tcttctcgct cattatagag acgcaaaatg actgtatttt
     2041 cgacagttgg gatagtcttt aagttttctg caaatgtgct catgtatcgt tgttagtcag
     2101 agttctttaa atatgtagtt cgtatataaa ctacctttcc tgcaacagta aaaacaagta
     2161 aattttggct tttttctaat tccatcaaag atgcttcagc ggcatttttc ccagcggaaa
     2221 aacattaaag ccaatttcat aaagtttttt gtgatcaaga tgatttcgtc catcaaaaat
     2281 aaaagccggt ttagccattg cttgaaagat tctttcaaaa tccagttcgc gatattcttt
     2341 ccactgggtg acaagggcca gtgcgtgagc acaatcagca gcttcatata cgtcttctac
     2401 aaaactaata tcaccctgta tttccttcag atcctgacgg gcattttcca gagcttttgg
     2461 atcatggacc cttacgcatg catgttcagc cagtaatttt tcacaaagcg gaattgcagg
     2521 agaatcccgt gtatctcccg tatcctgttt aaaggcaaaa ccaagcaatg taatggtttt
     2581 accggctaat gtattgaatt gttcctgaag aatgctctgg aaaaatctat cagtttgcca
     2641 ttcattgatc cggacaacac tttcccaata atctgcaact tccggcaatc cataatattc
     2701 acaaagatac gataaatgta atatatcctt acggaaacat gaaccaccaa aaccgatact
     2761 ggcttctaaa aattttgagc cgattcgtgt gtccataccg attgccttgg agacttccgc
     2821 aatatctgct tcagtacgtt cacaaagtgc ggaaatacta ttaattgaag aaatgcgctg
     2881 tgccaacatt gcattggcca ccagttttga taattcactg ctccatacgc tggtcagcag
     2941 tatgcgttcc tgcggaaccc agtgtgcgta aagtgccgcc accgtttctg ctgctttacg
     3001 gccatgctca ttttgttctc cgccaatcaa aactcgatcc ggatttgtta aatcttcgat
     3061 tgcagttcct tctgccaaga attccggatt tgaaacaacg gaaaattttg tcgaactttt
     3121 tcctgattct aaaattttag ccatcgcttc tgcagtacgt accggcaggg tgcttttctc
     3181 gactacaaca acttccggct tgcggcaatt ggctaatatt tcatgagcag ttttttccca
     3241 gaattgtaaa tctgcggctt ttcctgcacc ttctccaaac atttttgtag gtgtgttgac
     3301 tgaaacaaaa atgatgtctg catccgttgt tgccgcggcc acatcggtct gaaaaaacaa
     3361 attcctgccg cgtgcttcct ttacaacttt cagcagtccc ggctcataaa tcggcagttc
     3421 atcactgttc caacgggcaa tccgatcctg gttgatgtca acaaccgtta ttttgtgttc
     3481 cggacaattt ttggcaatca tcgccatcgt tggtccacct acataaccgg ctccgataca
     3541 aagtatatta ctcatttatt tagtcgtctg taaaaattat tggtttatta tctgcttcaa
     3601 tctgcaaaaa gtgaaggtgc caaatcgtcc ggaacagctt gtcccaaaag caaaaaattt
     3661 gttgctgcaa tattacttaa attgttattg taatcctgac caaacggttt aagtcgatag
     3721 tctccattat ataaaggatc ataaattaaa aacgggacag gattcagcga atgttttgta
     3781 ctaatttcca atgttccgtt ttgatttgaa atcagcattt catctgcatt accatgatct
     3841 gcggttatca ccagtaggcc gtttactgca tcgatggcac gtacaatatt gtccaatgcc
     3901 gcatccgcag cttcaactgc tcgaactgtg gcctggaaat ctcccgtgtg gcccaccata
     3961 tctgcatttg caaaatttat caaaccgtat tgatattctc cggaattgat aaattcaaca
     4021 gcttttttgc tgattcccgg agctttcatt gccggacgct ctgcaaaaga aggaattttg
     4081 tctgaggcaa tgaaatgata attttcctgc aaaggatcca gcggttcacg ataacctcca
     4141 ttgtagaaaa aagtcacatg ggcaaatttt tgcgtttcag agagtctaaa ctgtttgagt
     4201 cccagttcca aaatacgctt gccgaatggt tcttctacta caggtgttcc aaccaaataa
     4261 tccggcggaa tctggttatc ctgatcgtac tgtgtcatgc ccgcaaacag cacctctggg
     4321 cacctgtaac gctcaaaata tggaaaatca tctgcgagaa cagcctgtga aaactcagta
     4381 gcacggtcac cgcgaaagtt tgtgaaaatg agtgcatgct gatcttctat tctgccaact
     4441 gcttccccat tgcgaataat cacaaatccg ggaatgtcct gatcgatgat ctccggattt
     4501 tgttttcgaa aatgttctat tgcatcccgg attgaaggaa actggttttc agattgaccc
     4561 tgaacatgaa tctgccaacc agtttctatt ttttcccaat tgttgtcacg atccatcgtg
     4621 attacttcac gtccaccgcc gcttgcaaaa gcataatcga ttccgggacg ctgccccttt
     4681 aattccgaaa acagcttttc aaaaggttct gtaaaatcca aagcagattg aacaccaacg
     4741 tctcttccgt ccagcaaagc atgcagataa cagcgtctta taccagactg aaaagcatgt
     4801 cgaatgatgg cttcagtgtg atcaacgtgg ctgtggatat ttccatttga aagcaaaccc
     4861 agcaaatgca gtggcgtgtc gcgttccaca caattttgga tgattctgct taacaccgga
     4921 ttttcaaaaa actcaccgga ttgaagcagg ttctgtatca gtgtcggtcc ctgttccatg
     4981 accattccgg ctcccattgt catgtgccca acttctgatc cacccatatc tttttcgttc
     5041 ggcaagccaa catatttgcc gtgtgtccaa agctgggtgt atggacaacc gcgaatcaga
     5101 cgactcatta ctggtaaatt agccctgttg actgcattag tttcatctgc cgcaccaaca
     5161 ccccatccat ccaaaacaac gtgaatcaat ggtccgcgtc cagaaaaata ctgttggagg
     5221 tttttaagaa acaacattac cagtttgatt aaccttgtta ttcaaaacga agaatacatt
     5281 cttcaggcaa acgttcacaa agaaaattta aggctttgtc caaatactca gaatcccgac
     5341 attcaagagt caactttacg tcataatcag atcctgatac tttaggatat gaacccagca
     5401 tcagctgcgg aaattctgac atggttgcat caagtgaatc cgcgatttcg ccctcatctg
     5461 ctttgagata aatttgcttc aaaacaattg gagttgtccg gaaacgttcc ttgatactgg
     5521 caaaacggat cttcaaatac tctggaattc ccggaaaaac aaatatattg cgaaacttca
     5581 gttgtggacc ccggccttcg gcatactcaa tcagttcaga accttccgga atcattgcca
     5641 ttctgcggtg tgcggcagtc attttatcac catgataatt ttggatggct ttcatgatct
     5701 gaggtgcttc aaatagttct ataccaaaac ctttggcaat tgaggcaacg gttatatcgt
     5761 catgagtcgg tccgattcca ccacttgtaa aaacccagga gaactgctcc gaaaaatccc
     5821 gtaccgtccg tccaataatt tccggaatgt ctggaattgt gatgatttgc tggacctcta
     5881 caccaagaat tctcagttct ttacacaaat atggtgaatt aatatccgtc acttttccgg
     5941 ataaaatttc gttaccaatg atgatgatac ctgcagtttc caagacttaa ctcaaaacta
     6001 attgtttatt taattcgttt gataatgtgg tagccaaatt gagtttcgac tactccactt
     6061 acttcacccg gtttcaggtt aaaaacggcc tgatcaaact ccggaaccat ttgtccccgt
     6121 gtaaaacgcc ctaaatcccc tccttttggt ccggaaggac catcagaatg ttcccgcgcc
     6181 aattctgcaa aatcttttcc tcttttgaga tctttcagta tttgttccgc taatattctg
     6241 gcttcttttc gatcccgctt tttggtggca cgtagagcac cttcgtaagt tataaggatg
     6301 tgactggccg ttacaagctc aactaaaacc cgccgaaaaa ttaaatagcc aaaaggagag
     6361 tctaccggtt cacttatttg tcctacaccc aatttaagtg cgggcttgag aaagtccggt
     6421 aagctcggct gtttagcaga taataaagga agtttagact gttgaggcaa gtccgtaaac
     6481 tggttgatta aatcagaaaa cttgatacct tttcgacgtg cataatctgt cagtttttgt
     6541 gcaatttttt ctgcaccaga cttgtcatag aatatatttt gtttgggtgt cctcgcacct
     6601 tgataagcaa ccaccagcat atcaacacta acttcgcctg cttctgcatt atcagagtct
     6661 ggaatttttg caggatctaa ttcagttgtt gcgtgaggaa tatatttttt atttttttca
     6721 gcaagttttt tgttacgttc tgcaattaat ttttctgcaa ttgcaggatc aaaggccatg
     6781 gctgctttgc ctttgcgaat tattgtcact gcctgaatca ggtctccttt ttcgatggca
     6841 ttgactacat tcattccttc aacaacttca ccaaaaactg aatgcttgtt gtccagatgc
     6901 ggtgttggga catgtgtaat aaaaaactgg ctgccgtttg tgtgcgctcc agagttagcc
     6961 attgaaagaa ttcccggctt attatgtttc agttcaggat ggaattcgtc ggcaaatgtg
     7021 tagccggggc caccagtacc ggtagccagt ggatctccac cttgaatcat gaaatctttg
     7081 atcacacgat gaaaattcaa accatcaaaa aatcgtgttt tttttgcttt tccagttaca
     7141 gggtctttcc aatccttgct gccttccgca agtccgacaa agttggacac tgtcagaggc
     7201 gcacgtttaa aataaagccg caacactatt tcaccttttg acgtcttcat ttgtgcgtac
     7261 aggccctcct gaagcgttcc tccccaaacc gttcccgaaa aaaatattgc tgccgcgctt
     7321 agatacagtg ttaccgtgaa tgtcttaatt gaaagtcctg tatttatata aaaagtgttc
     7381 atgttgaatt ttgtaaacaa attgtaataa attaagaggt cctgtgattt aaagctttaa
     7441 aagagaaaaa ttaatctttc aggctagcct gtttacgctg caggtctcaa gttatttgtg
     7501 tttgcaaaaa cttatttctt tagcagacat tttcgaaaaa attctatttt gaagatcagg
     7561 aagcatttgc cagcaaaaac tctactgcac tccataaatc atcccgccaa taatccggat
     7621 ctgcttttgc cttgctgtcc tgaccaccat gtccggtgtg caccccgatt gaacatatcc
     7681 cccatttcga agctgctgca aaatcactgg tagaatctcc gattaaaatc gattcctctg
     7741 cagaaaatcc atgttcctct gctgcctgtt ccagtagtaa ggttccaggt tttcggcaag
     7801 cacaaggtgc tggcacttca cgacgctctc cgttttccca gtccggatga tgcggacaat
     7861 aatactgccc gtctaagtgt gctccggcct tttggagttc gtgttgcatt ttgtcggtta
     7921 ttgcctgaaa tgcagactca ctcaaatcac ctctggcaaa cgcagcttga ttcgtaataa
     7981 cgaaacacct aaagccctca gcattaagac gtgcaactgc agtagcgctt tttggaatca
     8041 tcacgaactg ttccggagtc agtatcggcc caggctcctc gttgattaca ccgtcacggt
     8101 ccagcagaac aacacgtaaa ttattgaaca aagatttaga cattgattaa attcagtcca
     8161 gaagttccaa ttgttgtttt cgttctttgt cggatacttt agcaagttca cgtacagcct
     8221 caaagaactg cgaccacttt cgtccttgtt tctcaaagag gtgcgtaaat tgttctactc
     8281 gactctggta gcgttgcaca ccaagcaaat gtgtattgtt caggtttttg gcaaaccagt
     8341 tatcgtatga aagtacttta aatgcttttt tatctatttc ataattttcg cgtaattgat
     8401 cataaagcac ctgtttgtta cggagctttt ctgcaactga aagttctgat gcaaacaaga
     8461 ttttcatttt atcataagtg gttttaatca gttgttggaa caacttacga tcttttctgg
     8521 cattcaaata ccactggtaa atttcttttc cgtcaccatc aaaaaaatcc gaattactgg
     8581 actgaataag aaattgccgt aggccctcct gttcaacaaa aactgcaaat gactcattga
     8641 aggaagtatc attgttgaca aacaccacct ggtgggccat ctcatggatc aggactgcta
     8701 tcaattcggc atcgcgccgg tgaataaaag tactaagaac cggatccgaa aaataattcg
     8761 gcagccaggg tttgttaagc caaccgagtg tggaataggc tgttaccggt cccaccgaaa
     8821 catccagttg ttgttgctcc atttcttccg catataacct ggcatcacct tcatcaaaat
     8881 agcctcgata ttcctgacag ccaacaaacc agtaacacca tttgtgtgct tttaattcca
     8941 gctcaggagc tgccgttacc accatggtca cataagaacg tcccagatct acatagccgg
     9001 tgtagccttc attttcagga agtgccattt gactgactgc aaaagcccgc acgctctcca
     9061 ccaatttcag tttccgtttt agtttcggag atgtatcagg agaatccagt aactcacgaa
     9121 tatcttgctt acggttcagc aagtcgaggt gtccggatgc ggcctgccaa taaaatccta
     9181 agtctgagca accacttaag aaaatgacag acccaagagc caacaataat aaatagcctg
     9241 aacgcacagc aataatttgt ttgggaaaag ttagttcaag aattgtaaaa attcctttgc
     9301 aggatttaaa attccagcat gtttggcaag caaattgtta tctgaatcaa gtattacgta
     9361 taacggaagg gcaaccgttt ggaatctgct gatctgcatt tgttgattgg cggcagcctt
     9421 gtctccacca tcagtataaa gctgtacaag tacaaactcc tgctgaaaac gagccattac
     9481 atccggatga gaaaatatgt ttttttccat ccagcgacag tttacgcagg tatatccggt
     9541 aaaatcaata aatacccgtt tgttttcagc ttttgcctgg atcagggcat cagccaaatc
     9601 attctgccat ggtaaagaat gtgacactgc ttctcctgaa gttcccgaac tagttgcaaa
     9661 accctgcctg ctttctgata atgttcgcag aaccggaggc aaatatgtat ccacccacgg
     9721 attaagcggt actccaaaca atccccgcag cagatatatt cccaaaaatg caaaaaggaa
     9781 agctgcagta aatcccgtac cacctgtttt ttcaacacga acatgatgta ttttaacagc
     9841 acctaacaaa aacaatgctc caatcacaca aagtacaacc caggccgcca gcacaaaatc
     9901 tttatcaaga acaccccatt gccaaaccaa atccgtgtta ctgaaaaatt tgaatgccgc
     9961 agctaattca agtaaaccca aaacaatttt caggtgctcc atccagtttc cggactgtga
    10021 acgcatcgtc tggataagcc gtgggaaaag tcccagcaag aaaaatggaa aggcaaatac
    10081 tgttgagaaa acaagcattc caacaatcgg ccaaaaccac attccctgtg aagcggcaat
    10141 cagcaaagtt cctacaaact gcacggtaca ggtgaaagac gtcaatgtga aagccagccc
    10201 catggccagt acccctgctg ttccactcaa cttcctggac caacggtctg caaggtttcc
    10261 aaatcctccc ggtgcattca attgaataaa tcccatcaag ctgaaggcaa aaagagtgaa
    10321 cattacacca attgccagat taacccagcc atttgtggca aactgtactg ctcctgcagc
    10381 acctaaaaca acagaaagca gcatacctgt tactgtatac gttcctacga ttcctgctcc
    10441 aaacagagcc gctaaacgta atgtttcttt ggaacctgct tcgccctgtt tagtaaaaaa
    10501 agcaactgtg accggaatca tcggaaacac acacggtgtc agcaatgcta acagacctgc
    10561 aatgactgcc agaccgataa aaccccaaaa accttccttg agcgcatctt cgatttctga
    10621 aacttcttca ggaggtgagt ctactgcaaa ctgtggaatt ttgtattcag tacgcacagt
    10681 ccctgtgcca agtggaattt ggagtgaaaa cagttcagtt ttgggtggaa gacaaatgcg
    10741 ttcgctgcat gcctgatatc gcagtttccc gcctttgttc aggacagttc cgccagaaat
    10801 atcatctgga atgagaatat tttggaaaag ggtggctttc atcaggtgat agctcaacac
    10861 catatccaaa accgggtctg ctgcaataat tggattactt tcgtaaaccg ggccgatcaa
    10921 aatcagcgga tcagcctcaa atgtcaatga agtgggaata ggggcaaaat cgccttcacc
    10981 gggaataaca gaaaagacgt gccatccaga ttccggatgg atttgtaaat cgacaataat
    11041 acgggcatgc tctcccggac gcggtttttc aggttcgagt cgtacctgga atgttacaac
    11101 tgattctgga attactacat catcatcaaa ctgagcaatc gtggtatgcg gaaacccgga
    11161 agtgaacagc aaagacaagc acagtaacag caggagttga cgcaaggccc tgtaaatact
    11221 tgatttaaat aaactgcggg ggggcataat ttgaagtcaa tagaattggc tgtaaattgt
    11281 acagatgaga ttcgggaaag gacacctctg caacaatttt cgaaatattg ttgatgaaaa
    11341 cttttggcaa gcagtttcca gcaagattca gctcaattta ttcagcactt ccgacacgca
    11401 gcggctccat gcgcatcgca gggcttccgt tccgtaccca aagccgtacc cgttcgactg
    11461 ttacaccgtt cggttttgtg agactggaaa gttcttctgc ataaagaatg tcttcagacc
    11521 tgaactcaaa aacatcgctt cgagattcaa gtgggctgga cagcagcaac agcattttcg
    11581 gattgtagtg tggacgcaac gctccacaaa aaggagttga atctttctcc aaagtttttg
    11641 aacgtccact acgcttttgt atctcgaaat aattaccttc ttcgagtagc tttaccaggt
    11701 tcatgcattc ctcaaaagat gattataaat tttccggacg caattttcgc agaacttttc
    11761 ctgcttgcca catttcctga ctgctaactt cctcaagctc cttttccagt ttttccctgt
    11821 aatcattttg agagtttgag tttatggata tttttgcttc agttccatcg agaacactct
    11881 ggtaacactt ttcaaccaca ggcttaataa catcccggaa acgcggtgcc caatcgagtg
    11941 ctccccgctg agcagtagtc gagcaattgg catacatcca atccattcct ttttccgcaa
    12001 ccagaggata aagactctgc agggcttctt cgattgtttc attgtaggct tcagaaggag
    12061 aatgtccatg ttctcgcagg acctcatatt gtgcgagaaa tgctccctgc agcagtccca
    12121 tcaaaacaca tcgctcgccg gtgagatcac tatgaacctc attttcaaat gtagtttcaa
    12181 aaaggtgtcc ggaaccaatc gcgaatgctg tggaaataca tctttcgcgc gcacgtcctg
    12241 tataatcctg atgaattgca aaagaagcat ttattccgcg tccttcttga aaatgtgtgc
    12301 ggactgtcag accgcttccc tttggagcta ccaggattac atccacattt tcgggtggta
    12361 taatgctcgt atcctggtga aagacgattc cgaaaccatg tgaaaagtaa agtgcatcac
    12421 cttcatttaa gctggcttta accactggcc atgcagctat ttgtcctgca tctgagagca
    12481 aatattgaag aattgtccca cgctgagccg cttcttcggt ttcgaaaagt gtttctcctt
    12541 ctagccagcc atctgccaga gccttgtccc agctcctgcc tttacgcagt cctaaaacaa
    12601 ccttgaaacc ctgatcccgc atattcagac cctgccctcg accctgggga ccatagccca
    12661 aaattgcagt gacttcattc cctagaatgt ttttgcattc ttcaatcgga taatcagagc
    12721 gctcaacgat ggtttccttt attccattaa tttcaatatc cttcattctt tctccacaaa
    12781 gttagtaatt tgtttaatta ttatcgacag tctgacttaa gtaattacta gtcacactat
    12841 gctgaataaa gtaaaaaaag tcaatacaat cagctattgt gcgctgatga gagtaaacaa
    12901 cagaggactt gttcatactg tcgctttagt ctcagcagaa gatgagggca gaacaaccag
    12961 ggagacttca gattccaaag cagaaaaatc tattgttcta tgttcttttg aatagtcatt
    13021 taattctgtt agccggagca atatttcttc acctgttgca ggaagattta gtcggggaca
    13081 cacgtcattt ctgacacatg acaaagcatg cttgactgca atccaaaccg acaggcagag
    13141 catcaaagga ggctctccta ctgccttact gcgacgaatg ttgatttgat gatgcggatt
    13201 gtggatggtg tccacattga aaatttctgg aagatcctga atgtttggaa ttttataggt
    13261 cgtcggagaa tatgtcaaaa gttcaccttt ttcagaatag cgcaaatctt catttgtcac
    13321 ccaacccaca ccctgaataa aacctcctat aatttgcccg cggtcaattc caggattgat
    13381 cgattcacca atatcaagca gtaaatctga acgctcaagc ttaaggtgac cggttaaacg
    13441 gtcgatgagt acctctgaaa ccgctgcccc agtcgtgtaa taaaaaaatg gttcaccctt
    13501 ctctgtatca cgattgaaat ttatttcagg cgtactgtaa aatcctctct cgcccagact
    13561 cactctgtcc ataaaagcca gttttacaag ttcccggaaa gtgagtctat tttctggatt
    13621 cctgtcatca aaaactgaac tattgccacc ggattttcct gcttcaaaac aaatgtgttc
    13681 cggagcagga tcaagtccct gcttcgccgc aaagtaactg cttgcaaatt ttgtcaaatt
    13741 cttcctgatt ttcctacatg cgttgactgc agcaaatccg ttcaagtcag ttccagcgga
    13801 agcagcagtc ggtggagtat tgttgttttt ctctgtggat gtaatcatca tccttacatc
    13861 atcataagaa atggagaatt catccgccac caattgcctg attttggtgt tcagtccctg
    13921 ccccatttcc gtaccgccgg tagaaacctg caccgtaccg tctttgtaga tattcaccag
    13981 ggcattcccc tgattaagaa attttgtggt gaaggaaatt ccaaacttca ttggagttaa
    14041 agccagtcca cgcagatgcg tttccgactg acgattgaaa tcctcaactt cagtcaagcg
    14101 ctgccgatat cctgatgttt caaccagttg atcaatgatt tccggaagaa gatgattacg
    14161 gacaatctgt ccgtatggcg taacgttgcg ttcatcatgg gaatagcaat ttagacgccg
    14221 aatctccaat gcatcttttt tcaaaaaaat ggcgatttcc tgaattacat tctcaatatt
    14281 tgccatgcct tgcggtcctc cgaatccacg gaaagctgta ttaggcggaa agtttgtttt
    14341 acagatcgtt ccattaaaaa tcagattcgg aatgaaatag gcgttttctg agtggaaaag
    14401 ggtacgctcc atgattgcag tcgacaaatc agctccggca ccgccgttgg aaaaaatatc
    14461 gaatttcact ccggttatct ttccgtctgc tgtgaaagct actttgtaat gaattttgta
    14521 tggatgacgc ttcccagtgg aacgcatatc ttcgtctttt gtgtaagcaa tccgtgcagg
    14581 tctttttgtt tttgcgacta ccagtgcaac catcactgca ggaatcactg cctgcgtttc
    14641 cttgccgcca aaagcaccgc ccatacgttt gcaaacacaa accacttcgt taaatcctaa
    14701 tcctaaagcc tcagcgataa cttcctggat ttccgttgga ttctggcttg aagaatgaat
    14761 ctgtatttca ccgtgttcac caggccatgc caaagcagct tgagattcca gataaaattg
    14821 ttcctggccg ttgcagataa atgttccttt caaagtgtgt tctgcctcac tccatgcctt
    14881 ttcaaaatta ccctgtttga accgacgtgt cgtgcctata aactgttttg cagaaattgc
    14941 ttcctcgatg gtgaaaacag gaacaagttc cttaatttct agacgcagct ttttcttggc
    15001 ctgccgaatt gcctccctgg actcaccggc aataattgca atgggctgac ccacatattc
    15061 aactacacct tccgccaaaa aacgttcatc ctgaatgacc ggcccataaa gattatgacc
    15121 aggaatatca gcatgtgtaa aaactcccgt aataccgtcc aattgagcca gttcttcaga
    15181 attgagattt ataatttcgc catgggcaac aggacttcct ataaaatcaa caaccagctc
    15241 atttttcgtg aatggtaaat catcaacgaa taaagcatct cctgtaacat gacctactgc
    15301 tgaatcatgc gcaacctgtt ttccaaccac tttcatgcgg tctcttttct gtgctgccgg
    15361 actttcgata aaatcagatc tcagttcatt ttgtgcagac ggtaaatcat ccacaaacaa
    15421 atcatcattt gttacacttg acgccgcaga aacattcgta acctgattcc cattctcttt
    15481 catgcagcct cttttttgtt ctggttgtca gagcattcat gaaaaaactt cagcaaaatg
    15541 ttttctgcca gctgcaacct gtaatccgaa gaggcacgga catctgaaat tggacttatt
    15601 tcatctcgag ccagttttcc agcttcatgg aaaattcccg gcagcatcgg cttaccctgc
    15661 aaaaactctt ctgtctttgg caagcgcaac acaacaggtc ccacgccccc aaaagccagg
    15721 cgggcatttt gtattttccc tttttttaac ctcagcaaaa tcccggcggt aaaggtagat
    15781 atatccaaat ccttacgttt ggaaactttg tagagtttca gcacagtctc cggttcaggt
    15841 aaattccagc gaattcttgt gataagttca tccggtttca tttcagtatt tttatagccg
    15901 tgataaaacc ggttaatatt tacccaacgt gtaccatgtg atgcagtcaa ttccatttcc
    15961 gcgtctatta catgtagaaa tggcagggag tccgcaattg gtgacgcatt tgcaatattg
    16021 cctgccagcg ttgccgcgtt cttgatttgt cgtgaggcaa aaattgagat gatttcgtca
    16081 agctcaggca ggtgttcctg acaaaattca ccaagctccg tccaagttac cttggcgcca
    16141 actgtcacag ttttgttttc aatctcaaga atttccagtc cagtcaaatg cgtcaggctc
    16201 ataatacaat ttggctccag ccgctctttg ttcatctgta cagaaatatc cgttccaccg
    16261 gcaacaacgg tgacatgctt gtttttttgc ttaaattcaa gagcttcttc taaatacgca
    16321 ggcacaaaaa actgtacttg ttgacctgct ccattccatt ttttctcgta ctcacaataa
    16381 acaggctgga caacatgttc cataaaatcc gcaagtattg ccttcgcatc gtaacatttt
    16441 gaagcctttg gtatgacttt ctgattcaaa gacaagccgg catcaataat ttgttcgtaa
    16501 cccgtacagc ggcacaaatt cccggttaag ccgtcttgca cggaatgtct cgttagtgtt
    16561 tcaccggttt caagcattgc agccagtgcc attacaaatc ccggtgtaca ataaccacat
    16621 tgtgacccag cggcatcaac cattgctttt tgtacaggat gtattatttg agcctcatcc
    16681 ttcaagcctt ccactgtaat gacatgagaa caatccagtt gatataaata ctgaatgcaa
    16741 ctgtcaattc cctcatagag caactgagag ttccggactc gcccgataag tacggtacag
    16801 gaaccacaat ctccttctgc acacaccacc ttggtaccaa ccatacccaa atcataacgt
    16861 agaaaatcag acagtgattg aaatccacgc tttccggaaa ttttgtaacg ccggccattc
    16921 acataaatta gaagataatc acgcatattc tcttttctcc atcacgtgtt ccgtgccgtt
    16981 tttgttcatt ccggtcatca tctgaccaat caaagttaca gaggcctctt catgcgaaag
    17041 agttccaaca atacgtccgt caaaaataac tgcaatgcgg tctgcgacca tcaataattc
    17101 atccagatcc tctgatatcc aaagcaccgc cagaccccga tctcgtgcct ggcatatttg
    17161 ctcacggatg aaactggttg catgaatatc caggccacgg gtgggttgtg cagcaagcag
    17221 taatttcaat ggacgcgaaa tttcacgggc aagaatgact ttctgcatgt tacctccgga
    17281 taaaagtcca accggtgttt tttccgtagg tgcccggata tcaaactcct gcattttttt
    17341 ttgtccatag tcgaagatat ttgaaagctt gagcagctgt cttgaacaaa attgggggtt
    17401 ttgaaaatct cgcagcaaaa aattgtaggc gattgacatt ccggacgaaa caccaaacct
    17461 tttacgatcc tccggaatgt aaccaactcc catgtcaatc atttcataca cagtgggttt
    17521 gttcaaaggc acatcttcaa actgatactt gcctcttgtc ggagaccgca gcccactcaa
    17581 aacgtcggtc aattccggtt gaccgttccc ggaaatcccg gccattccca gaatttctcc
    17641 ggcacgaata tcaagtgaaa tccctttcaa tgccgggtac cccaaatcat tcaccgcatg
    17701 aatatccttt aaagctactg tcaaattacc aaactttttc ttttgctttt gagaaactga
    17761 accatcccca tctttttctt caacagttgt atcccgtaga aattcagaaa cttttactaa
    17821 gtccttagct tccaaattac tgatcatcaa atcagctaat tcttgcggat tggtttcatc
    17881 tttcttcaaa gttgccatca cctttccacg actcaaaacc gttattcgat gcgaaagtgc
    17941 catcacttcc ttcattttat gtgtaataaa aatgatggaa tgtttttcat ttaccatctt
    18001 ctccatgatt tcgtacaatg cctctgattc ctgaggtgtc aaaactgcgg ttggctcgtc
    18061 aagaatcagg attcgtgctc ccctaaacag aactttgaca atttccacgc gctgctgttc
    18121 acccactgaa agttcctgaa ttttcttttc tggatcaaca tataaatcat agcgttcgct
    18181 tatttcccgg atttgttgat gaacttcttt caacttaagc ttccccactc cgggtaaacc
    18241 aaggatgata ttttcggcaa cagtatgatt tcgaaccagc ataaaatgct gaaataccat
    18301 gccaatgctt ttttcaatag cctgacgagg attagtaaaa ttcacttgct ccccaaagac
    18361 acgcatctct ccagcatcgg cagggtagag ccctgcaatc atattcatca aagttgattt
    18421 acctgcacca ttttcaccca gtattgtgtg aatctctcct tcctccaatg taaaatcaac
    18481 actatcattg gcaacgattc ccggaaaact ttttgtaatt ctactcaatt gcaaaggtag
    18541 ccgttgttcc gaatgagact tttctatttc agatgaattc atattgcact cagagtatta
    18601 attttaatgg tttcgaaaat aataaacatt cccgaacttg cttaacatta tcgtttgact
    18661 gtatacattt ttcgactgca tcacaactca atgctaaagc ttttaacagg tgcttaagct
    18721 caaagcgtat tttctttatt taattgtgat attaaccttt tactcgagta accggcaagt
    18781 cttttaagag attcaataaa tgaaaaattc ggagtaaagg aaggatggat ggggattccg
    18841 gtgtctgcac ttaaaaagaa ctaaactttt tcggcttaac ctctgttttg aattaatccc
    18901 agaacagttt ctggaacttg attagcctga gctatcattg cagtactggc ctgaagcagg
    18961 atttgatctt tagtgaggtt cgacatttct gcagccatat ctgagtcacg cagggttgac
    19021 tctgcctgtg tgatattttc ttctgcaatc ttaagactgt tcatgttgtt ttccagggca
    19081 tttttttgaa atgaacccaa cttaccacgc tggacagata tttcttcaat tacccggtct
    19141 acaataattg aagcgtcttg agcaccctgt gacgaagtta cttcaatctc agcaaggcta
    19201 gaaaatccgc tcttgttatg aatgccgtta cccataagac tggtgcggac gtttgcaaat
    19261 gagaatgtag gattagattt tttatctaga ccaacctgga attctttcga catttgggaa
    19321 acatgtacgt agccttctat tttcggatta tttggaccac caacaatatc ctcatttttc
    19381 tcctcaatca gctctgttcc gatcagttca cctgactcat tcaaaattgg agtatttatg
    19441 agtccaatct ctctatcata cttgatcgtt attccttcag cttccatccc ttccaaagta
    19501 gtcagcagct gaccttctcc caaagtcact tcatcgttga ttgttccttc tatatttata
    19561 ccgctttcag acttctgggc tatattagca tcatcagaaa gtatacctgc aactgagctt
    19621 gtcactgaaa aagaggcaga gtccccaaat ttgttgtgtc gaacaagcaa ttttttgtct
    19681 ggtgtctcag ctatataaag atctaaacca ctttcttcag ctgcctgact caatgcaaaa
    19741 atgataagtt cacgaatttg tgatgaaatt tctgcttgag gaaacagtaa tcgatcctga
    19801 tcagcatttt ttatcaattg ctcaatctct tgtcccaacc gaccgtcctt tgtgtttaag
    19861 gtcaaatttc tgccaccttc attcagaacg atttgaagtc cattacgaat attgttgata
    19921 ttgattgcaa tcgatcctgt ttttttggct ctagttgcaa cttgctgaat atcaactttc
    19981 cagccttgtt caggggaggt tgaagctgta gcctcagctg agacaaaccg caggtaattc
    20041 cctacagctg taccgttaac acccatactg ccgtcaagca gtctcttacc gccaaattca
    20101 gtattttccg aaattctatc aatagaactc agcaggttat caatttcctg ttgatctgca
    20161 gacaacatgt cagagctgtt tattgcttcg ttctgagcgt gtacagtcag ctgttttatt
    20221 tttatcagca gatcacttac ttcatttaat gcgccttctg ctgtttgcag cattgatact
    20281 gaggaagaag cgttgttata tacctgctgg agtcccacaa tatgtccgcg taatctttct
    20341 gaagaaatca gagaggctgg accatcggca cctctgttaa tctttactcc ggaactaagg
    20401 cgctctatgc cgcccttcac cttgttgaag tgatttgagg cgtgtctcaa agtgtttaat
    20461 gaggatggat tttgtctaat tttcattgct gaagaaaaac tttaagttca ggtaaaaaat
    20521 atgcttgagc tgttgtttca acaagcatgc tcaagactta atcagtagaa aactctcgtg
    20581 tctttaatcg gcaataatca ataaatcttt aacaatatta caggtttttt tagttatttt
    20641 gttttttacc cattcccagt aaaaaaatct ccacgctttc ggctctggaa cttttcggtt
    20701 tacataattt aaccttagca aatgagtttc taaaagcatt gcgcaattca tccagaggtg
    20761 caccttgaaa agctttcacc agcagcattc cctgtggtct caacaattcc tctgccagct
    20821 gcaaaacctg ctggttcagt gcataagatc gctgggcatc agtagcacga attccggtag
    20881 tttttggcgc catatcgctc aggataacgt caaccatccc accactcatt tccagatcct
    20941 ttgccgtcag ttcaaaaata tctgcttgaa gtactttcac atgtgcaggc agcgaatggt
    21001 tcactggctg taaatccaca ccgagaacct ctccctcttc acctactttt ctgctcgcgt
    21061 actgaagcca tgaacctgga ctgcacccta ggtccaacac caaattacct ttacggataa
    21121 tcctgtgttt tttatctatt tcttccagtt tgtaggcaga acgggcaaca taaccgtttc
    21181 gctttgcctt gtgaaaataa tgatctttaa tttttttcaa agcaaaccct tatcagcaaa
    21241 actggtatag ttatcaccac ccacaatcac atgatcgagc acaggtattc ccaccagttt
    21301 cccgacctct accagacgtt ccgttatccg gaaatcctct tgtgaaggtt ccggatctcc
    21361 ggaaggatgg ttatgaacgc atatcaaagc tgcagcacga tgttcaatag ccgaagcaaa
    21421 aacttctctt ggatgcacca ggcttttatt taaaatacct ttggtaacat cttcttccgc
    21481 caaataccgg tgtttattat ccagtaaaac aattataaat tgctcctgtt tttgacgctc
    21541 caactgttga tgaaaatgat taaaaatttc acggctggaa ttaaactgat cctgcggctt
    21601 cagcttttca attccataat ttctgcacat ttcccgtagg gcctccaggc gcaaaatcag
    21661 atttgaatca agaggctgct gattttccag ttcccgcaaa cgttcgcggc tccaatcttt
    21721 gagcggtgcc tccgcattaa gtgaattttg caatgctttt gcttcttccg gatttgaact
    21781 cagcagccat tgcattaatt ccggaatatc tgcagttgaa attttgtttc tctgtatttt
    21841 ttcttcaatt gccatttttt ctgagttccc ctcagctgca taccaatggc attctaaact
    21901 cagttcacaa ttatcacaat ctggtaaatt accgcaaaca cctgccaaac cggcatgact
    21961 cccttcaccg gaaaatgctg cagacagacg atctgcttca aaggcagttt caaaaacgtt
    22021 ttgctcctcg catatttcca accatttttt tggatgtaag ctggcatttt ttgctgattc
    22081 tgaattagat ccatgtgaaa aacgccgcca tgcgcgataa ccactttgac tgtgagggac
    22141 tgctattcct tcctgccaga gccacttcag tgcgttccat ggtttgaggt cgggatatat
    22201 tttccaccac aaactcaacg ttttaattga tttcaattgc atgggattta ggtccgtttt
    22261 cttcacaata tccataaaaa ccataacggc actggtcagt tgttcccgct gtgaatcatt
    22321 aagtcccgtg catttttcca gcattcgcaa aacctcatct ttacggcaat aaacaggatc
    22381 aagaagatta ataccatcgt aaagcggttg cagtcccgca aatcttgtca attcttcact
    22441 cacttcaaaa agtggtagaa ttcgatccaa tagtttttca ccaagatttt tccgactcaa
    22501 caacctgata ttgcttgagt tcagttgggc cgctgtccaa gggtgcatcc ttaaggaagc
    22561 gccaagtttt ttttgtagta tctgaaaaaa gtgctcgtgt tccgccattt ttaaccggtg
    22621 ctgtgttgtc ctttgtgcac tttagataaa ctcttcattt cattcaattt aacgaaaatg
    22681 aattgtaact cttatgcttt tgttacagaa tgcactaaag aaacaagaag tttattatta
    22741 gtatctttgc tctgtttgtc tttagcctat caggttttaa gcttattact attaatttat
    22801 tggtcaccaa tagcggtctg gcaaaaagtc tgaaatccac acagtcattt cgactgttgt
    22861 gaaaaagctt aataaaatta atgtagtagc ttatgcagga tttctcgctt cgctcaaaat
    22921 ggcgcggttt taggtttttt tacgacggta tcttctatgg tttggttgta gagtaattat
    22981 agtgtttttt cgaaatgtat gatattatac tgatattatt gattattatt tctaattcaa
    23041 gtttataatt aaggatctca tgttagacaa aataggaacc cttttgggaa tgttgatggg
    23101 agtatcgctg gtaattttcg gtattatttg gcccgatcac ctttctaatt attacatgta
    23161 ccagttccgg gaatttgagc tgagtctgga ggccttaaaa gtatctcagg caccaatcga
    23221 agaaatacaa gccctgaaag caagttttaa aatgtttcag gaatcatggt ttggttctat
    23281 ttcacgtttc gccgatttaa aatctctgct tattgtactc gggggatctt atgctgcaac
    23341 cctaattgcc ttccgttttg gtgatgcaat gcgtgcaatt ctgtttatcg ccaaagcttt
    23401 tttaacaggc aaagccgaca aggattttct tgaagtttat catactgtga tcagtctctg
    23461 tgaaaaacgt gccaataaag agttaattac tgatgaggaa atcagtgccg taaaaaacaa
    23521 agaccttcaa aactggctgc aggattttat cgctgtcgat ttggttacag aagaaatgat
    23581 tgaggaaatt gtgcgttctg aaattgaaat gtacaactat cgttcttttg aagaaataga
    23641 catgctggaa tttatgggac gtgccgcacc ggcttttggc atgattggta cagttgtcgg
    23701 tctgattatg atgctgggca gtgttggcgg agaaggtggc gctgacatag cggggttgat
    23761 gggaggcatg gcagttgcga tgataactac actctatggt gtcttgcttt cgcagctgat
    23821 ctttttgcca atcgcttcca agcgttttca actcaaagag acgcaggttt tgttgatgga
    23881 aatgatcagg gaaggtctgc tctacctgaa acgcagggag cttcctgaaa ctgctgccaa
    23941 ggatctagta atatatcttc ctcccaaact gcgtaagaaa gtaatgaagg aggaacaggc
    24001 tgcaatgggt caagggttgg ggttgtaaca tgagtcgaag tgccagttca gagggcgggt
    24061 ggataacgac atttgcggat ttaatgactc tgttattctg ctttttcgtt ttgctgaacg
    24121 ctctttccac tcaacctaaa aattgcggag gccttgaaca gtttctcag
//
LOCUS       DTVD01000170            1769 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_30822_length_1769_cov_545.161610, whole genome
            shotgun sequence.
ACCESSION   DTVD01000170 DTVD01000000
VERSION     DTVD01000170.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30822_length_1769_cov_545.161610"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="tpiA"
                     /locus_tag="EYO68_05910"
     CDS             <1..504
                     /gene="tpiA"
                     /locus_tag="EYO68_05910"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIC07376.1"
                     /translation="ECGVQWCIVGHSERRILFGESDEFIRSKTETLLKHGIRPILCIG
                     ETLEQRESSNQEEVVVIQLGKGLSGLAVDDQKRCVVAYEPIWAIGTGKTATPEQANSM
                     HKTIRKELESLASPEAAENMQIIYGGSVNAENAKTLLSQSEIDGALVGGASLKTDAFP
                     QIVAASS"
     gene            531..926
                     /gene="secG"
                     /locus_tag="EYO68_05915"
     CDS             531..926
                     /gene="secG"
                     /locus_tag="EYO68_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIC07377.1"
                     /translation="MFELILVIHIIACISIIFFVLLQSGRGAELGAAFGSVGQANSVR
                     GSMTGVGKITTGVAIVFMLTSLSLAYLSSDTARDSVVRDLAPATTTIPVAEQMLIPDE
                     DTQAEKTPEIPPAELEQKVAPPIESSSGN"
     gene            1247..1714
                     /locus_tag="EYO68_05920"
     CDS             1247..1714
                     /locus_tag="EYO68_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07378.1"
                     /translation="MKFLKQISLNEWLSTLIIASAAFLLLGAMTALWSNPFFMRMTEV
                     SGWDYIILSFESLMIGLFFGIRAPHCATKKAGIGGVLGFVGFGCSICNKILLLLFGSS
                     FLLSYFEPVRHYVGALGLLLFSYALIQRWLLRLAVLNENRLQKETDPDSEVVL"
BASE COUNT          495 a          339 c          390 g          545 t
ORIGIN      
        1 gaatgcggag ttcagtggtg tatagttgga cattccgaac gcaggatttt atttggtgaa
       61 tcagacgaat tcatccgtag caaaacagaa actctgctca aacatggtat acgcccaatt
      121 ctttgtatag gagaaacgtt ggagcaacga gaatcaagca atcaggaaga agttgtggtt
      181 atacagttag gcaagggatt gtccggatta gcggtagatg accagaaacg ctgtgtcgtc
      241 gcctatgaac cgatttgggc tataggcaca ggaaaaaccg ccacaccgga acaagctaat
      301 tctatgcaca agacaatcag gaaagagctg gaatcactgg ccagtccgga agcagcagaa
      361 aatatgcaaa tcatttacgg tggaagtgta aatgcggaaa atgcaaaaac tttactttct
      421 cagtctgaaa ttgacggtgc tcttgtaggt ggagcaagtc ttaaaacgga tgccttccca
      481 caaattgtag ctgccagcag ttaattataa ttctcatttg accagttttt atgtttgaat
      541 taattctagt catccatatt attgcttgta tttcgattat cttttttgtt cttcttcaat
      601 ccggacgcgg tgctgaactt ggtgcagctt ttggtagtgt cggtcaagca aactcggtac
      661 ggggaagcat gacaggtgtc ggaaaaatta caaccggtgt ggcgattgtt ttcatgctga
      721 cctctctctc tcttgcatat ctttcctcag atactgcccg tgattcagtg gtacgggact
      781 tggctcctgc cacaacaacg attcctgttg cagaacaaat gctcattcct gatgaagaca
      841 cgcaggcaga aaaaactccg gagattcctc cagctgaatt agaacagaaa gttgcaccac
      901 ctatcgaatc ttcatccggc aactaagact actggtcaca aaactccgct ctgactgtta
      961 cgcattttgc acactttgag gttgttttgc tgatctaaat ctgactacct gaattttgtt
     1021 caacttttga ctgccatcgt acaatccgat gatccccgtc ttatctcaga gcctttccaa
     1081 ctgtaataat aaaatcaatt atgactgaag cacaaatttc gggaaatcca gtattctaaa
     1141 ctgaattgat tgaaaatgag cttgttgcat ttattaagaa tctgatccat tgtggttcgg
     1201 aataatttga gtgcagaacg gaatgtaatt tcctaaagga ctgctaatga aatttttgaa
     1261 acaaataagc ttgaatgaat ggctgagtac cttgattatt gcttctgcag cgtttttact
     1321 gttgggagca atgactgcac tttggagtaa tccatttttc atgcgtatga ccgaagtgag
     1381 tggctgggat tatataatcc tcagttttga atcacttatg attgggctgt tcttcggtat
     1441 tcgtgcaccg cactgtgcca cgaaaaaagc aggaatcggt ggagtattgg gatttgtcgg
     1501 gtttggctgt tcgatttgca ataaaattct tttactattg tttggctcta gttttttgct
     1561 ttcttacttt gagccggttc ggcattatgt aggcgcattg ggtttacttt tgttttccta
     1621 tgcccttatc cagagatggt tgttgcggct agctgtttta aatgaaaacc gtcttcaaaa
     1681 ggagacagat cctgattcag aagtagtatt ataaaatatg cgaagaataa tttaataaat
     1741 tagatttgca tcaatatctg aatctgtgc
//
LOCUS       DTVD01000171            7148 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3099_length_7148_cov_306.960665, whole genome
            shotgun sequence.
ACCESSION   DTVD01000171 DTVD01000000
VERSION     DTVD01000171.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_7148_cov_306.960665"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="EYO68_05925"
     CDS             <1..159
                     /locus_tag="EYO68_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIC07379.1"
                     /translation="VGWMLREVGKRELEIEEEFLQEHYQEMPRTMLRYAIERITDARR
                     KMYLKGQI"
     gene            373..1599
                     /locus_tag="EYO68_05930"
     CDS             373..1599
                     /locus_tag="EYO68_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC07380.1"
                     /translation="MFELSETQKQLQSTAREFAKKEIAPRAAEVDRTEQYPWENVELL
                     TKQGFMGMTIPEEYGGPGMSYLDVVLVIEEIAKACGVSARIVVEANMGAVGAVMKYGS
                     EVQKKLAAEHVLSGDKPAICITEPGAGSAATEMATRADHKGDRYILNGTKHWITGGGV
                     SKFHLIFARVFENEEPQGIAGFIVLQGTKGLKVGTREPAMGLRGIPETQIICEDLEVP
                     EEMVLIPPEGLRRGFAGLMNAYNGQRVGAGTVALGIAAGAYELALDYSKQREQFGRPI
                     CEFQGLQWMLVDMHTQIEAARLLLHKAASSGSGSSGQVAPDVPGFPDMLEAAQAKIFS
                     SEMAIKVTNDALQIFGSAGYSRNMPLERMARDARMFTIGGGTAQILRTVVASALLNRK
                     LPQTRDGHFKLAAKEA"
     gene            1736..3397
                     /locus_tag="EYO68_05935"
     CDS             1736..3397
                     /locus_tag="EYO68_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIC07381.1"
                     /translation="MANSAEIIGKRLYHSGCRRAFGIPGGEVLVLMEGLRKSGIDFVL
                     TKHENCAGFMAEGGFHADGAPGILVATIGPGVANAVNVVANAFQDRVPMIFLTGCVDA
                     DEALTYTHQVFDHGELLKSVTKASIKVVDGAVDVSIDKAIAIALDGQPGPVHVDLPIR
                     LASKSQPASTMIVRTFPSHTAPAEGTELETARQWLREAKRPLIIAGVDVLNQNAVCVV
                     KEFVRDFGIPLITTYKAKGVLPEDDPLALGGAGLSPLADQYLLPLVRESDLIILAGYD
                     PIEMRSGWRDPWDDGTRVIEFCAVPNTHYMHQADLSFVGHVGEGLKTLRKEASANPVW
                     KNGEPEKVRRDLKSIFSGTVTSGAGEEWGPGAVVDVVRKALPRNGVATVDSGAHRILL
                     SQMWECYEARSLLQSSGLCTMGCAVPLAMGFRLAAQAGMSGANSPVPDVPAVVAFVGD
                     AGMEMVLGELATLRDLKLPVVIVVFIDESLALIELKQRKSKLQNLGVDFGATDFPAVA
                     RALGGIGHWISSRNELQVALDGAFERETFTLLACIIGEKVYDKRF"
     gene            3483..4952
                     /gene="mpl"
                     /locus_tag="EYO68_05940"
     CDS             3483..4952
                     /gene="mpl"
                     /locus_tag="EYO68_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIC07382.1"
                     /translation="MLLPPVFLNAAAFCYPMSLLYSNSKLIHFTGICGTGMASLAVLL
                     KQRGHHICGSDENVYPPMSEFLSQNNITVKSGYDAKNLEPVPDLVVIGNALSRGNPEV
                     EAILESKLPYISMAELLKEYFIRGKTSLVVTGTHGKTTTTSLLAWVFETAEKKPGFMI
                     GGIAENFGASCLDGSGNCFITEGDEYDTAFFDKRSKFLHYLPDQLILNNLEFDHADIF
                     DSLADIKKAFRLLLRLIPGKGLIVANGDDENVKSVLESSFSPVLKFGLGKSCDIQAVN
                     LKSSEAGMSFEVLDNRAESSTQNQEFELNLFGTYNVLNALGVIVLARYNGIADKTIQA
                     AFDSFQSVRRRQELRGEVNGISVYDDFAHHPTAIRETVTAIRQKHPGRRLITVFEPRS
                     NTSVLKIHQDALIDSFKKADEVILTTPHRLERTPVEQRLEVLEVLKSLIKNKIPAYEF
                     PQVEGIIEHLKSNCRSGDVVLIMSNGKFDNIHQRLLEEL"
     gene            4971..6479
                     /locus_tag="EYO68_05945"
     CDS             4971..6479
                     /locus_tag="EYO68_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIC07383.1"
                     /translation="MAIREHTIYDCIMRNALNLSGETALVSGDLRLTFSEIPIHVNCL
                     ARGLLNTGFEKGDRIAVLLNNCAEYALLLAACTRVGVVAVCLNTRTSAGEMRMVLEST
                     KPKALLFQTSFEQQAEELRDLFPHRQFYCIDEASILSTSFDQLLDENPAPLTEPQPGV
                     DDGWLIIPTAAVDGVPKGALLSQRNVLASAAVHLHHFGRDAIKGHLLALPIFHVMGLT
                     SAWATFIGGGKNVVLKQFDDKEAVRLIDAEQLTYFGSFPPILERVLDSAKETGSVLKS
                     LQVVYGLEGPQNIKRLQEETNAVFWTGFGQAETTAFVTASPASERLGASGQSTLINSV
                     AIVNELDEQVPTGEEGEIVVRGENIFLEYWNMPEATAYAQRNSWHHTGDIGRLDDDGF
                     LWYVKRKAEKELIKTGGENVYPGEVESVLLKHAEIKDCCVIGVPDKTWGEAVKAVCVL
                     KKGSKLSEKEVSDYVGGQIAGFKKPRKVEFVEKLPRENDEVDREAVKAKWEE"
     gene            complement(6489..>7148)
                     /locus_tag="EYO68_05950"
     CDS             complement(6489..>7148)
                     /locus_tag="EYO68_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC07384.1"
                     /translation="DAEGEKTVSEIKATGGEAIYTHADISKAADCENMVKAAEDQFGK
                     LNVMFNNAGIMDSRDDNALVTEEEVWDLTMAINLKGVFLGCKYGIAAMQRAGGGSIIN
                     TASFVGLMGAATPQVAYTASKGGVIALTRELSVIHARENIRVNALCPGPLRTELLMKF
                     LDTEAKKQKRLVHIPMGRFGEAKEMAQAALFLASDESSYTTGTEFVVDGGITSAYVTP
                     E"
BASE COUNT         1936 a         1434 c         1848 g         1930 t
ORIGIN      
        1 gtcggctgga tgctccggga agtcggcaaa agggaacttg aaattgaaga agagtttttg
       61 caggaacatt accaagaaat gccgcgtaca atgctccgtt atgcaatcga gaggattacg
      121 gacgcaaggc gaaaaatgta tttaaaagga caaatctgaa ctcaaaaaca cataaatcta
      181 caggagtggt tgatgatttg acaatcactt aaccctcctg tcattccggg cagagcgtag
      241 aggagacccg gaatccaggg gaattaccgc gagttctgga ttcccgtccc caatcaggtt
      301 gaggacaagc tttcacggga atgacaaaat gaaaaatcag cccgaaatca accatttttg
      361 aaaagaaaaa tcatgtttga actatcagaa actcaaaaac aactgcaatc aaccgcacgg
      421 gagtttgcca aaaaggaaat tgcgccccgt gccgcagaag tagatcgtac ggaacagtat
      481 ccgtgggaaa atgtggaact cttgaccaag cagggtttca tgggcatgac gattccggaa
      541 gaatatggag gccccggaat gtcgtatctg gatgtggtac tggtaattga ggaaattgca
      601 aaggcctgcg gggtttcggc tcggatagtt gtggaagcga atatgggagc agttggagca
      661 gtaatgaaat acggttcaga agttcaaaaa aaattggcgg cggaacatgt tttgagcggc
      721 gacaaaccgg cgatctgcat caccgaacct ggagctggaa gtgcggccac agaaatggcg
      781 acccgcgccg atcacaaagg cgaccgctac attctcaacg gtacaaagca ctggattacg
      841 ggtggcggcg tttccaaatt tcatttaatt tttgcccgtg tttttgaaaa tgaggaaccg
      901 cagggtattg ccggatttat cgttttgcag ggaacaaagg gattgaaagt cggaacacgc
      961 gaaccggcga tgggtttgcg cggaattccg gaaacgcaaa tcatttgcga agatttggaa
     1021 gttccggaag aaatggtctt gattccgccg gaaggtttgc ggcgcggatt tgccgggttg
     1081 atgaatgctt acaacggcca gcgtgttgga gctggaacgg tagcgctggg cattgccgca
     1141 ggtgcctatg aactggcctt ggactattca aagcaacgtg aacaattcgg acgcccgatt
     1201 tgcgaatttc agggattgca gtggatgctg gtcgatatgc acacgcaaat tgaagccgca
     1261 cgtctattgc ttcacaaagc tgctagctca ggttccggta gttctggaca agtcgcaccg
     1321 gacgttccgg gttttccgga catgctggaa gcggcgcagg ccaagatttt ttcctctgaa
     1381 atggcgatca aagtcaccaa tgacgcactg caaatatttg gttctgccgg atattcccgg
     1441 aacatgccac tggaacgtat ggcgagggat gcacgcatgt tcaccattgg aggcggcaca
     1501 gcgcagattt tgcgtactgt agtggcttcc gcattactga atcgaaaact cccgcaaaca
     1561 cgggacgggc attttaaatt ggcggcaaaa gaggcataat tcgcaggtat gtaattccgc
     1621 aatgtccggc acgttgtcag gctctacagc tttttgcaaa aaataaatgt tggttattgg
     1681 caaaatatat ttttggtttt tgtgagtttt ttgcaaaata aaatgaaagg ttgtaatggc
     1741 aaattctgct gaaataattg ggaaaagact gtatcactcg ggatgtcgac gtgcgtttgg
     1801 gattccgggc ggagaggttt tggttttgat ggagggcttg agaaaatccg gaattgattt
     1861 tgttttaaca aagcacgaaa actgtgccgg gttcatggcg gaaggcgggt ttcatgctga
     1921 cggtgcgcca ggaattctgg tagcgaccat tggtccggga gttgccaatg ctgtgaatgt
     1981 agtagcaaat gcttttcagg atcgggtgcc gatgattttc ctcaccggtt gcgtggatgc
     2041 tgatgaggcc ctgacctaca ctcatcaggt ttttgatcac ggtgaattac tcaaatcggt
     2101 tactaaggcc agtataaaag tcgttgatgg cgctgtagat gtgtcaattg acaaggcgat
     2161 agcgatcgcg ttggacggtc aacccggacc ggtgcatgtc gatttgccga ttaggctggc
     2221 ctcgaaatca cagccggcaa gcactatgat tgtccgaaca tttccttcac ataccgcacc
     2281 agcggaagga acagaattgg aaactgcccg acagtggctc cgggaagcaa aacggccttt
     2341 gataatagcc ggtgtagatg tgttgaatca gaatgcggtg tgcgttgtga aggaattcgt
     2401 gcgggacttc gggattccac tgatcacaac ttacaaagca aaaggcgtgt tgccggagga
     2461 tgacccgctt gctctcggcg gtgccggact ttcgcctttg gccgaccaat atttgttacc
     2521 gctggtacgg gaaagtgatt taattattct ggcaggatac gatccgattg aaatgcgttc
     2581 aggctggcgt gacccctggg atgatggcac gagggtgatc gaattttgcg cagtaccgaa
     2641 cacgcattat atgcaccaag ccgatttgag ttttgtcgga cacgttggtg aaggtttgaa
     2701 aaccttgaga aaagaagctt ctgcaaatcc ggtttggaaa aatggagaac cggaaaaagt
     2761 caggagagat ttgaaatcga tcttttccgg aaccgtaacg tccggtgctg gtgaggaatg
     2821 ggggccggga gcggtagttg atgttgtgcg aaaagcttta ccacgaaatg gagttgccac
     2881 ggtggacagc ggagcgcaca ggattttgct gagccagatg tgggaatgtt acgaagctcg
     2941 cagcttgctg caatcttctg gactttgtac gatgggttgc gctgtaccgt tggccatggg
     3001 ctttcgtttg gcggctcagg ccggaatgtc cggtgcgaat agtcccgtac cggacgttcc
     3061 ggcggtcgtg gcctttgtcg gagatgcagg tatggaaatg gttttaggtg aattggcgac
     3121 tttgcgggat ttaaaattgc ctgttgtgat tgtggtattt atcgatgaat cactggccct
     3181 gattgaactc aaacagcgca aatcaaaatt acagaatctc ggagtcgatt ttggtgcaac
     3241 cgactttcca gctgtagccc gggctttggg tggtattgga cactggattt caagtcgaaa
     3301 cgaactacag gttgcactgg atggagcttt tgaacgtgaa acatttacgt tactggcttg
     3361 tataattggt gaaaaggttt acgataaacg cttctaattg gggctgtcag accactgcga
     3421 tcctcatttt ttgctcaatt aggttcgcgg attctgtttc gtagtacagt ttatgaaagt
     3481 ctgtgctgct ccctcctgtt tttcttaatg cagctgcatt ttgttacccg atgtctcttc
     3541 tatattcaaa ttccaagctc attcatttta caggaatctg cggaaccgga atggcttctc
     3601 tcgcagtgtt attgaaacag cgtggtcatc atatatgcgg ttctgatgaa aatgtttatc
     3661 ctcccatgag tgaatttctt tcacaaaata acataaccgt aaagagcgga tatgacgcaa
     3721 aaaatttgga gcctgtccct gatttggttg taatcggaaa tgctctatcg cgaggtaatc
     3781 ctgaggtaga ggcaattctg gagagtaaac tgccgtatat ttcgatggcg gaactgctga
     3841 aagagtattt catacgtgga aaaacatctc ttgtggtcac tggaacacac ggaaaaacaa
     3901 ctacaacgtc tttattggcc tgggtatttg agactgcaga gaaaaagccg ggcttcatga
     3961 ttggaggtat tgcagaaaat tttggagcca gttgtcttga tggaagcggt aactgtttta
     4021 tcactgaagg tgatgagtac gatacagcat tttttgacaa gcgttcaaaa tttttacact
     4081 atctgccgga tcagttaatt ctgaataatc tcgaatttga tcatgcagat atttttgatt
     4141 cacttgcaga tattaaaaag gccttcagac tgctgctgcg tcttattcca gggaaaggtc
     4201 tgattgttgc caatggagat gatgaaaatg taaaaagtgt tttggagtcg tctttttcgc
     4261 cggtacttaa atttggttta ggtaaatctt gtgatataca ggcagtaaat ctgaaatcca
     4321 gtgaagcagg aatgtctttt gaggtacttg acaatcgtgc tgaaagttct acccaaaatc
     4381 aggaatttga actaaatctt tttgggacgt acaacgtcct caatgcattg ggtgtgattg
     4441 tactggcacg gtataacgga attgcagaca aaacaattca ggccgctttt gattcattcc
     4501 agagtgttcg cagaaggcag gaattacgtg gagaagtaaa cggaatttct gtttatgatg
     4561 actttgcaca tcatccgaca gcgattcgtg agacagtgac tgcaatcagg caaaaacatc
     4621 caggacgccg cttgattacc gtttttgagc cacgaagcaa taccagcgta cttaaaatac
     4681 accaggacgc cttgattgac tctttcaaga aagccgacga ggtgatttta actacacccc
     4741 atcgtttgga acggactcct gtagaacagc ggctggaagt tttggaagtt ttgaaatccc
     4801 tgattaaaaa caaaattcct gcctatgaat ttcctcaagt agagggcata attgaacatc
     4861 taaaatcaaa ttgccgttca ggagatgttg tcttaattat gagtaatgga aaattcgata
     4921 atattcatca acgtttgctg gaagaacttt aacaaattga aaaggaaata atggctatac
     4981 gggaacatac tatttacgat tgtattatga ggaatgccct gaatttatcc ggagaaacag
     5041 ctctggtttc aggcgatcta cgactcactt tttcggaaat tccaattcac gtcaattgtc
     5101 tggcacgtgg attactgaat accggttttg agaaaggaga caggattgct gttctgctaa
     5161 acaactgtgc cgaatatgca ctgcttttgg ctgcctgtac tcgtgtcggg gtagttgcag
     5221 tctgtttgaa cacacgaaca agtgccggcg aaatgcgcat ggtgcttgaa agtacaaaac
     5281 cgaaagcctt gctatttcaa acgagtttcg aacagcaggc tgaagaacta cgtgatttgt
     5341 ttccacatcg tcaattttac tgtattgatg aagcttcgat actttctaca tcctttgacc
     5401 aactgctgga tgaaaaccct gcaccgctca ctgagccgca acccggtgtg gacgatggct
     5461 ggttgattat tccaactgcg gcagtggatg gtgtacctaa aggtgctttg ctcagtcaac
     5521 gaaatgtttt ggcctctgcg gcagtgcatc tccaccattt tggacgtgat gccatcaaag
     5581 gtcatttact tgcgttgccg atctttcatg tgatgggtct gacttctgcc tgggcgacat
     5641 tcatcggtgg tggtaaaaat gtagtgctta aacaatttga tgataaagaa gctgtgcgtt
     5701 taattgacgc agaacaactg acatattttg gttcgtttcc gccaattttg gaacgtgtac
     5761 ttgactctgc aaaagaaaca ggatcagttt tgaaaagttt acaggttgta tatggtttag
     5821 aaggaccaca gaatatcaaa cgcttacagg aagaaaccaa tgctgttttt tggacgggct
     5881 ttggacaagc ggaaaccacg gcttttgtga ctgcgtctcc tgcttctgaa cgtcttggtg
     5941 cttccggaca gtcgactttg attaactccg tggcaattgt taatgagtta gatgaacagg
     6001 tgccaacagg ggaagagggt gaaattgttg tgcggggaga aaacattttt cttgaatact
     6061 ggaatatgcc cgaagcaaca gcatatgccc agcgcaacag ttggcatcac accggtgata
     6121 taggacgctt agatgacgat ggattcttgt ggtatgtcaa aagaaaggca gaaaaagagt
     6181 tgataaaaac cggcggagaa aatgtttatc ccggtgaggt tgaatcggtt ttgctgaaac
     6241 atgctgagat taaagattgc tgtgttatag gagttcctga taagacttgg ggcgaagcag
     6301 tgaaagcagt ttgtgtgctt aagaaaggat ccaagctttc cgaaaaagaa gtaagcgatt
     6361 atgttggcgg acaaattgcg ggttttaaga aacccagaaa agtagaattt gtggaaaaac
     6421 tgcctcgtga aaatgatgag gtagatcgtg aggcagtaaa agccaagtgg gaagaatgag
     6481 gtaagaaatc attccggagt aacgtaggcc gaagtgatcc cgccgtccac cacaaactct
     6541 gttccggttg tataagatga ttcatctgaa gctaaaaata aagctgcctg tgccatttct
     6601 tttgcttctc caaaacgacc cataggaata tgtaccagtc ttttttgttt tttagcctca
     6661 gtatcaagaa acttcatcag cagttccgtg cgtaaaggtc ccggacaaag ggcattgacc
     6721 cggatgtttt cacgtgcgtg aattacggat aattcacgag tcagggcaat cacacctcct
     6781 ttgctggcag tgtaggcaac ctgtggtgtt gccgctccca tcagtcccac gaaagacgct
     6841 gtattgatta tcgatccccc tcctgcacgc tgcatggcgg caattccgta tttgcagccg
     6901 agaaacactc ctttaaggtt aattgccatc gttaaatccc aaacttcttc ctctgtaaca
     6961 agcgcattgt catcacggct gtccatgatt ccggcattgt tgaacatcac attcagtttg
     7021 ccgaattgat cttctgcggc tttaaccata ttttcgcagt ctgctgcttt ggaaatatcc
     7081 gcatgtgtat aaatcgcttc gcctccagta gctttaattt ctgaaactgt tttttctcct
     7141 tcagcgtc
//
LOCUS       DTVD01000172            7043 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3180_length_7043_cov_320.853463, whole genome
            shotgun sequence.
ACCESSION   DTVD01000172 DTVD01000000
VERSION     DTVD01000172.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_7043_cov_320.853463"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1308)
                     /locus_tag="EYO68_05955"
     CDS             complement(<1..>1308)
                     /locus_tag="EYO68_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC07385.1"
                     /translation="EIIYHIVTAIILAIIIIVVAVPEGLPMMIAVVLSMNMRKLLKAK
                     VLVRKLLGIETSGSLTILFTDKTGTLTQGKLTVSELLLGNAEHLQSYKEIPENLRETV
                     SFALRNNTPASIDTGDPENPKIVGANPTGQALLRFLGADLAKQDDVKVKVNIPFNSAY
                     KFSATQVEGSQNLTMVKGAAEIVLAGCTHYLDVDGNKVELDSEKLKEEMLGLSKRAMR
                     LIGLAVSNQDLGEENVLPSDLTLVGIFGLRDEMRKESKTAVETVRRAGIQVVMITGDA
                     KDTAQAIAREVGILEKEQALVLTSKELGEISDEELIKILPDMRVVARALPADKNRLVK
                     LAKSMNWVVGMTGDGVNDAPAVKNADVGFSMGDGTDMTKESSDIVILDNNFISLTNAV
                     RYGRTLLKSIRKFLIFQLTVNVAAILVAFLGPFFGIDLPLTMT"
     assembly_gap    1309..1408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1409..2210)
                     /locus_tag="EYO68_05960"
     CDS             complement(<1409..2210)
                     /locus_tag="EYO68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HIC07386.1"
                     /translation="MKFSYPGLSDNAVSESRKIYGANVVTTQEAEGFFDKLRTNLKDP
                     IIVILIVALAVTVFLAAMGFAPWYEGLGIAFAVVMATLIATWSEYSNENEFQRLLEEA
                     SKVKVKVFRNSTMVEILIDDLVVNDLVLLQPGDTVPADGYLLTGEIELNESALTGESE
                     TVKKTGGADEKHSEAEEKYEMSRAALVVDGEAVMKVLEVGDKTKYGVTLKELTSAETR
                     PSPLQEKLATLGRQISRFGYVGALFIAFSFMFNHIFLEGGGWEIYFSKP"
     gene            2452..2802
                     /locus_tag="EYO68_05965"
     CDS             2452..2802
                     /locus_tag="EYO68_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC07387.1"
                     /translation="MKHGFTLVEVMFANAMLLILVAGFAKITEQTFEQIQLKTLKNEL
                     ASHLDEWTLTKLNNPLNAGNYQENISLSGKNAELHWQVKQVNPNLITLLFQVKTSDTV
                     PKVLAQWQTALKTQ"
     gene            2799..3326
                     /locus_tag="EYO68_05970"
     CDS             2799..3326
                     /locus_tag="EYO68_05970"
                     /inference="COORDINATES: protein motif:HMM:PF07963.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC07388.1"
                     /translation="MMKKRQGCLEYNSAYTLIEVMLVLAIVSVVLISAQRPLLEFHRI
                     AVLEQQKEVLRGDFQRFLLLLKSELIQAGYALSSGSETAVEISTHSLVFKADRNRDGD
                     VADTREKIAYRYDPETKVLFRKSGNAAYQTLIESIYDLKFEIAQAPPQTCIKVSVQVI
                     IDAPEQETVLCQLVW"
     gene            3501..3920
                     /locus_tag="EYO68_05975"
     CDS             3501..3920
                     /locus_tag="EYO68_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIC07389.1"
                     /translation="MKYLHTMIRVQNLDSALDFFIDKLGMKEVRRKEVPEGKFTLVFL
                     AADDNESQLELTYNWDQNEPYSGGDNFGHVAYSVENIYDYCEKLEKKGVTILRPPRDG
                     KMAFIRSPDQISIELLQQGDPLPIQEPWSSMKNRGKW"
     gene            3922..4491
                     /locus_tag="EYO68_05980"
     CDS             3922..4491
                     /locus_tag="EYO68_05980"
                     /inference="COORDINATES: protein motif:HMM:PF09835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIC07390.1"
                     /translation="MKKKIEKIIQEKLINPVLHSRAPVSEVSLGVAVGVFLGLTPTVG
                     VQMYLVAVVWSIYRYIFRRHFNLPVGVAMVWISNPLTMVPLYYLFLVTGYWLLETQNG
                     LSYQYFADTLGRISETGGTWGIIVEGARFLLIDLGWPMIIGGFVYAVPGFFISYFLTK
                     SIATSHRKSMARIAGMSYEDWQTENETQH"
     gene            4475..5779
                     /locus_tag="EYO68_05985"
     CDS             4475..5779
                     /locus_tag="EYO68_05985"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07391.1"
                     /translation="MRLNIKRCMSQTSYSVIESAFPEDIITSGDYESAENLLNKKSIP
                     SEILAESFYRLGNRFYSSEQKQAAELAWQKSRELTDITAEFASITVPVSRNQKKYYLQ
                     TIGSVILLIVCLYVFVFTLFPREPEPFQFTTFRSTSGELSFWDEWWNTGRPVTRSMRQ
                     RFGPEQLWPMLQRTLENLFGTQNDQLSEDIREKLKRWLELSRKPQFSKGPVDYYALTG
                     RGLFEAREFEDALSTLNDGLHYAESTEQLEQLYQDLGTVYYYKGYKLQPNGLAKYDLD
                     AVRNSVESYEMALRFGEDPYLYGNLGWGYYLLGDYSSSIESSLHSLALKPDLNYARMN
                     LGIAQLKKGDYELAFSAYASLKQHSPELDEYEGGIRDLRELQQEFPGLHPFSNFIMGL
                     LYLQQGRHKEARTAWQKFVSQKFSEPFWQHRARLLLKKMETE"
     gene            5800..>7043
                     /locus_tag="EYO68_05990"
     CDS             5800..>7043
                     /locus_tag="EYO68_05990"
                     /EC_number="3.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIC07392.1"
                     /translation="MQKNIVAEPQLAGQGKQKIEWVKRNMPILRQIEDDFRKHQYFSG
                     LRVLVCIHLEAKTAYLAQVFAAGGAEVAVTGSNPHSTKDDVVAALAEEGMHVYARYGA
                     TPEEMRQYMNRALDVRPNIMIDDGGDIAELVHTERQELLPEVLGACEETTTGVLRAKA
                     RAKAGVLEFPVVLINDARCKYLFDNYHGTGQSVWDGVMRTTNLVVSGKTVVIIGYGWC
                     GKGCAERAQGLGANVIVCEVDPIKAADALMHGLRVMPLNQAVPEGDIILTVTGGKHVI
                     RQEHYERMKDGVILANAGHFKIEFDLDALLNLSVEQKEMRHNITGFLMADGRWLNLLG
                     DGDLVNIACADGHPAEIMDTSFALQALSGKYVALHHEDLNKDVYRVPDEIDEEVARLK
                     LKGSGVSIDELLPEQKAYLESW"
BASE COUNT         2005 a         1424 c         1542 g         1972 t
ORIGIN      
        1 tgagtcatgg tcagcggtaa atcaatacca aaaaatggtc ccaaaaaagc cactaaaatg
       61 gcggcaacgt tgacggtcaa ttgaaaaatc aaaaattttc taattgattt taatagtgtc
      121 cgcccataac gaactgcatt tgtgagtgag ataaaattat tatcaagaat gacaatatcg
      181 ctcgattctt ttgtcatgtc cgttccatca cccattgaga aaccaacgtc tgcgtttttt
      241 acggctggtg catcattgac tccatctccg gtcatgccga ccacccagtt catggatttt
      301 gccaatttaa ccaaacggtt tttatccgcg ggaagtgcac gggcaacgac cctcatatcc
      361 ggaagtattt ttattaactc ttcatcagaa atttcgccaa gttctttgga agtaagaaca
      421 agcgcttgtt ctttttctaa aattccgact tctctggcaa tcgcttgagc agtatctttt
      481 gcatcgccgg taatcattac tacctgtatt ccagctcgcc ggactgtctc cactgctgtc
      541 tttgattcct ttcgcatttc gtcacgcagg ccgaagattc ccactaaagt caaatcggaa
      601 ggaagaacat tttcctcccc caaatcttga tttgaaacag ccagtccaat gagccgcatt
      661 gcgcgtttgg aaagccccag catttcttct ttgagttttt cggaatccag ttcgaccttg
      721 ttaccgtcca catccaaata atgtgtacat ccggcgagca cgatttctgc ggcccccttg
      781 accatagtta aattttgcga accttcaacc tgtgtagctg aaaatttata agcactgtta
      841 aaaggaatat ttacctttac ttttacatca tcctgcttgg ctaaatctgc gccgaggaaa
      901 cgcaataaag cttgtcctgt tggattagcg ccaacgattt tcgggttttc cggatctcca
      961 gtatcaatcg aagccggggt attgtttcgc agggcaaagg acacggtttc acgcaaattt
     1021 tcgggaattt ctttgtaaga ttgtaaatgt tcagcattac ccagaagaag ctctgaaact
     1081 gttaatttac cttgagtcag tgttcctgtt ttgtcggtaa acaggattgt caggcttccg
     1141 gatgtttcaa tgccgagaag tttccgcacc agaactttcg ctttgagaag cttgcgcata
     1201 ttcatcgaaa gcacgactgc aatcatcatt ggcaaccctt ccggaacggc tacgacaata
     1261 atgatgattg ctaaaataat agctgtcact atatgataaa ttatttcann nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ggtttgctga aataaatttc ccatcctccg
     1441 ccttccagaa aaatatgatt aaacatgaat gaaaaggcga taaacagcgc tccaacataa
     1501 ccaaaacgac tgatttgccg gccaagagtt gccagttttt cctgcagtgg agagggtctg
     1561 gtttccgctg aggtcaattc tttgagtgtt acaccatact ttgttttgtc tccaacctcc
     1621 aaaactttca ttacagcttc accgtccacc accagggcag cacgtgacat ttcatacttc
     1681 tcttctgcct cagaatgttt ttcatctgca ccccctgttt tttttacggt ttcagattca
     1741 cccgtaagtg cagattcatt caactcaatt tctccagtga gcaaatagcc atctgcgggt
     1801 actgtgtctc caggctgcag aagaaccaaa tcgttgacta ccagatcgtc aattaatatt
     1861 tcaaccattg tactgttacg gaagactttt acttttacct ttgaagcttc ctcaagcaaa
     1921 cgctgaaatt cattctcatt actgtattca gaccaggtgg cgatcaaggt tgccatcacc
     1981 acagcaaacg caattccgag tccttcatac cacggagcaa atcccatggc ggccaaaaaa
     2041 accgttactg ccagggcaac aatcaaaatg acgatgatcg gatcttttaa gttggttcgc
     2101 agtttatcaa aaaatccctc tgcttcctgc gttgtaacaa cattcgcacc gtaaattttt
     2161 cgagattcag aaaccgcatt gtcgcttagt cctggataac taaatttcat tgattgactc
     2221 tcttggtaaa ggttgggaaa atatcattaa gattttccca aggctcgaaa tgactccgaa
     2281 cctaaaatgt ctttggatta ttcttttttt catttccaga ttgctaattc aggtgtgggg
     2341 gtggatttca aactatttac tgaactacct cggcataaca attatcaaag gcaagtttga
     2401 catataagag tagtaaaagc aaaaaatcat tttgaaagat atagaatcag gatgaaacat
     2461 ggatttacgc tggttgaggt gatgtttgca aatgcgatgc tgctgatttt agtggcagga
     2521 tttgccaaaa taacagaaca gacatttgag caaatacagc tcaaaacgct caaaaatgaa
     2581 ctagcctcac atttggatga atggactctg acaaagctga ataacccatt aaatgcagga
     2641 aattaccagg aaaatatttc tttgtcaggt aagaacgccg aactgcattg gcaggtcaaa
     2701 caagtgaacc caaacctaat tacacttttg tttcaggtaa aaacaagtga tacggttcca
     2761 aaagtgctgg cgcagtggca aacagcgctg aaaacacaat gatgaaaaag cgtcaaggtt
     2821 gccttgaata caattccgct tataccttaa ttgaagtgat gcttgtcctg gcaatcgttt
     2881 ctgttgttct tatttcggca caacggcctt tacttgaatt tcatcgaatc gcggttctgg
     2941 agcagcagaa agaagtgctg cggggtgact ttcaacgttt ccttctgctg cttaaatcag
     3001 agctgattca agccggttat gcgcttagtt ccggaagtga aactgctgtc gaaatttcta
     3061 cccattcgct ggtcttcaaa gcagaccgca acagggacgg agacgttgca gatacacgtg
     3121 aaaaaatcgc ctaccgttat gatccggaaa cgaaagtact cttccgaaaa tcagggaatg
     3181 ctgcttacca aacattgatt gaatccattt atgaccttaa atttgaaata gctcaagctc
     3241 cacctcaaac ttgcataaag gtatcggttc aagtaataat tgacgcaccg gaacaagaaa
     3301 ccgttttgtg ccagttggtc tggtgaatct taaatgatgg tttagtgaaa aggctgaaat
     3361 cctaattttt tgatttcgac cataggaaaa aatcttaatg atattagtgt agtagattat
     3421 acaagatttc tttcttcgct cgaaatgaca ctgttttagg gctttttcca agttcagttt
     3481 aaatgataag aaaggaaaaa atgaaatatt tacataccat gatccgtgtt caaaatcttg
     3541 attcagcact tgattttttc attgacaaac ttggaatgaa agaagtgaga cgcaaggaag
     3601 tccctgaagg gaaatttacc ctggtttttc tggcagcaga tgataatgaa tctcaactgg
     3661 agttaaccta caactgggac cagaatgaac cctacagcgg cggagataat tttgggcatg
     3721 ttgcctactc tgttgaaaat atttatgatt attgtgagaa actggaaaaa aaaggtgtaa
     3781 ccattttgcg tcctccaagg gacggaaaaa tggcattcat ccgtagtccg gatcaaatat
     3841 cgattgaatt actacaacag ggtgaccctc tacctataca ggaaccttgg tcttccatga
     3901 agaaccgggg gaagtggtag aatgaagaaa aaaatagaaa agattattca agagaaatta
     3961 attaatccgg ttttacattc cagggctcct gtttcagaag tgagtttggg agttgcggtt
     4021 ggtgtttttc ttggtttaac tccaactgtt ggagtgcaaa tgtatttggt ggccgttgtc
     4081 tggagtatct accgttacat tttccgcaga cattttaatt tgccggtggg agtggcaatg
     4141 gtttggattt ccaatccact cacgatggtg cctctttatt atttattttt agtaacagga
     4201 tattggttgc ttgaaactca gaatggtctt tcatatcagt attttgcgga tactcttgga
     4261 cgaatttctg aaaccggggg aacatgggga ataatagttg aaggcgcacg ttttttattg
     4321 attgaccttg gctggcccat gattatcggc ggttttgttt atgcagttcc aggatttttt
     4381 atcagctatt ttcttacaaa aagtattgcg acatcgcacc gaaaaagtat ggcccgcatt
     4441 gccggaatga gctacgaaga ctggcaaact gaaaatgaga ctcaacatta aacgctgtat
     4501 gtctcagacc tcttacagtg ttattgaatc tgcatttcca gaagatatta taacttccgg
     4561 cgattatgaa tctgctgaaa atttgttaaa taagaagtca atcccctctg aaattctcgc
     4621 tgagagtttc taccgtctgg gtaaccgttt ctattcttca gaacaaaaac aggctgcgga
     4681 actggcctgg caaaaatccc gtgagcttac agatattact gcagaattcg cctcgatcac
     4741 ggttccggtt tcccgcaatc agaaaaaata ttatttgcaa acaattgggt cagttatact
     4801 tttaatcgtc tgcctgtatg tctttgtctt caccttgttt ccgcgcgaac ctgaaccttt
     4861 ccaattcaca actttccgtt ctacttccgg ggaactttct ttctgggatg aatggtggaa
     4921 cacaggacgg ccggtaacgc ggtcaatgag acagcggttt ggtccagagc agttatggcc
     4981 catgctgcag agaaccttgg agaatctatt tggtactcaa aatgatcaac tgtctgaaga
     5041 cattcgtgaa aaattaaagc gctggctgga actttcaagg aaaccgcaat ttagtaaggg
     5101 acccgtggat tattatgctt tgaccggaag aggcttgttt gaggcaaggg aatttgaaga
     5161 tgcactatca actttgaacg acgggctgca ttatgctgaa tcaacagaac agttagagca
     5221 gttgtatcag gatttgggaa ccgtctatta ttacaaagga tacaaactcc agccgaatgg
     5281 tttggcaaag tatgatttag atgctgtcag gaattcagtt gaatcatacg aaatggcact
     5341 gcgctttggt gaagaccctt atttgtacgg aaatttgggt tggggatact atctgctggg
     5401 tgattattct tcctctatag aaagcagtct tcactcgttg gccctcaaac cggatttaaa
     5461 ttatgcccgt atgaatttgg gaattgcgca actaaaaaag ggggattatg aattggcatt
     5521 ttcagcatat gcatcgctca agcagcacag tccggaactt gatgaatatg aaggtggaat
     5581 ccgtgattta cgggagttac agcaggaatt tcccggtctt catcctttca gcaattttat
     5641 aatgggacta ttatatttgc agcagggtcg tcacaaagaa gcacggactg catggcaaaa
     5701 atttgtcagt caaaaatttt cagaaccctt ttggcaacat agagcacgtt tgctgttaaa
     5761 aaaaatggaa acggaataac taaaaacgac aaacaaaata tgcaaaaaaa cattgttgct
     5821 gaacctcaac ttgctggaca gggaaaacag aaaattgagt gggtcaaacg caacatgcca
     5881 attctgaggc aaattgaaga cgattttcgc aagcatcagt atttcagcgg tttgcgggtt
     5941 ttggtctgca ttcacctgga agctaaaacc gcatatttgg cacaggtttt tgctgcaggt
     6001 ggagcagagg tggcggttac aggcagcaat ccacattcaa ctaaggacga cgttgtggct
     6061 gcactggccg aggaaggaat gcacgtttat gcacgctatg gagcgacacc ggaagagatg
     6121 cggcagtata tgaaccgggc actcgatgtt cggccgaata ttatgattga cgatgggggt
     6181 gatattgctg aattagtgca tacggaacgt caggaactgc ttccggaagt gctgggtgcc
     6241 tgcgaagaaa ccactacggg ggttttgcgt gccaaagcca gggcaaaagc aggagtactt
     6301 gaatttccag ttgttttgat caacgacgcc cgctgtaaat atctgtttga caactatcac
     6361 ggtacaggac aatcagtttg ggacggagta atgcgaacca caaatttggt ggtcagcggg
     6421 aaaacggtgg tgattatcgg ttacggctgg tgtggtaaag gttgtgctga acgagctcag
     6481 ggtttgggag caaatgtgat cgtttgtgaa gtggatccga taaaggcagc cgatgccctg
     6541 atgcacggat taagggtgat gcccctgaat caagccgtac cggaaggtga tattattttg
     6601 accgttacag gtggaaaaca cgtcatccgc caggaacatt acgaacgcat gaaggacgga
     6661 gtaattctgg ccaatgccgg acatttcaaa atcgaattcg atttggatgc tttgctaaat
     6721 ctatccgtgg aacaaaaaga aatgcgtcac aatattaccg gttttctgat ggcagacgga
     6781 cgctggctta atctgctcgg agacggagat ctggtaaata tcgcctgtgc cgacggtcat
     6841 cctgcggaaa tcatggatac cagttttgcg ctgcaggccc tgtccggaaa atatgtggcg
     6901 cttcatcatg aggatttgaa caaagatgta tatcgggttc cggatgaaat tgacgaagaa
     6961 gttgccagac tcaaacttaa aggttctggc gtttccattg acgagttgct tccggaacaa
     7021 aaagcgtatc tggaaagttg gga
//
LOCUS       DTVD01000173            1728 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_32031_length_1728_cov_376.425583, whole genome
            shotgun sequence.
ACCESSION   DTVD01000173 DTVD01000000
VERSION     DTVD01000173.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32031_length_1728_cov_376.425583"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="EYO68_05995"
     CDS             complement(<1..764)
                     /locus_tag="EYO68_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07393.1"
                     /translation="MRLKIFEKMIYILLIYLIAGAVLFFFQRKLLYFPTGKIPHAYET
                     LTLENENETLKVIVLNSGREQALLYFGGNAETVVYNAADFITAFPLHTVYLLNYRGYG
                     GSSGQPTEAGIFADALALFDKVQKKQERISVMGRSLGSGAATYLASKRPVEKMLLISP
                     FDSIKSVAQNKFPIYPMFLLLKDKYDSIGRVKDISAKTIVLIAENDEVIPKKHSLRLI
                     SEFPPEQITVKTISDAGHNDISNKMEYYNHLKDFLN"
     gene            complement(785..>1728)
                     /locus_tag="EYO68_06000"
     CDS             complement(785..>1728)
                     /locus_tag="EYO68_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC07394.1"
                     /translation="EKVIPAMQQGEYCEMAAIASQSLEKGQAAAAQLGIPKAYGSYDE
                     LLADAEIDAVYIPLPNHLHVPWSIKALKAGKHVLCEKPIGLTTVEAEELLDEARKHPE
                     LKVMEAFMYRHHPQWQRAQQLVNEGKIGALQTIHSFFSYYNADPENIRNMAQIGGGGL
                     MDIGCYCISLARFIFGSEPQKVLGKLEYDPEFRTDRLCSGILDFGEGTSSFTCSTQLT
                     PYQRVNIFGTEGRIEIEIPFNAPPNVPCRMLHQNGDQLEEIVLEICDQYTIQGDLFSL
                     SVLNDTEVPTPLEDAFANMKVLEAVIRSSGKGAWTRL"
BASE COUNT          436 a          399 c          382 g          511 t
ORIGIN      
        1 ttattgagaa aatcttttag atggttatag tattccattt tatttgaaat gtcattatgt
       61 cctgcatccg aaattgtttt gaccgttatc tgctccggag gaaattcact gatcaggcgc
      121 agtgaatgct tttttggaat gacctcatca ttttctgcaa taagcacgat tgtttttgcg
      181 ctgatatctt ttactctgcc gattgagtca tatttatctt tcagcagcaa aaacatggga
      241 tagatcggga atttgttttg tgcaacactt ttgatgctgt caaaaggaga gatcagcagc
      301 attttttcaa cgggtctttt tgaagccaga taagttgcgg ccccgcttcc caggctcctg
      361 cccatcacag aaatcctttc ctgttttttc tgcactttgt caaaaagagc cagtgcatcc
      421 gcaaaaatgc ctgcctcagt cggttgtccg gaacttccgc cgtaaccgcg gtaattaagc
      481 agatagactg tatgcaacgg aaatgctgtt ataaaatcag ccgcattgta aacaactgtt
      541 tccgcgtttc cgccgaaata gagcagtgct tgctctcttc cggaattgag tacaattact
      601 ttaagtgttt cattttcatt ttccagtgtc agcgtttcat aagcgtgagg tattttgccg
      661 gtaggaaaat agagcagttt tctctggaag aaaaacagca ctgctccggc aatgagataa
      721 atcagtaaaa tataaatcat tttctcaaag atttttaatc tcatgatttg gtctgcactg
      781 gttgttataa cctggtccaa gccccctttc cggaactgcg gataactgct tcaaggacct
      841 tcatgttggc gaaagcatcc tccagcggcg tcggaacctc cgtgtcattt agcacggaca
      901 gtgaaaaaag gtcgccctgt attgtatact ggtcgcaaat ttcaagcacg atttcttcaa
      961 gctgatctcc gttctggtgc agcattctgc acggcacatt cggcggggca ttgaaaggta
     1021 tctcaatctc aattctgccc tccgttccaa aaatgtttac ccgctgatag ggcgtcagct
     1081 gggtggaaca ggtgaaggat gaggtacctt caccaaagtc gagaatcccg gaacagagtc
     1141 tgtcggttct gaattcagga tcatattcca gctttcccaa aactttttga ggttcactgc
     1201 cgaagatgaa gcgcgcgaga gaaatgcagt agcagccaat gtccatcaat ccacctccac
     1261 cgatctgagc catgttgcgg atgttttcag gatcagcatt gtagtaggaa aaaaatgaat
     1321 gaatggtctg cagcgcaccg atcttaccct catttacaag ctgctgtgca cgctgccatt
     1381 gcggatgatg gcgatacatg aaggcttcca ttactttcaa ttcgggatgt ttccgcgctt
     1441 catcgagcaa ttcttctgcc tcaacagttg tcaggccaat gggtttttcg cacaaaacgt
     1501 gtttgcctgc ttttaatgct ttgattgacc atggaacatg cagatgattt ggcagtggaa
     1561 tgtaaaccgc atcaatttca gcatccgcca gtagttcatc ataggaaccg taggcttttg
     1621 gaatgcccag ctgagctgct gctgcctgtc ctttttccaa gctctgagaa gcaattgcag
     1681 ccatctcgca gtactcaccc tgctgcatgg cgggaatcac tttttcca
//
LOCUS       DTVD01000174           23448 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_323_length_23448_cov_468.555423, whole genome
            shotgun sequence.
ACCESSION   DTVD01000174 DTVD01000000
VERSION     DTVD01000174.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 23448)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23448)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23448
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_23448_cov_468.555423"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            330..965
                     /locus_tag="EYO68_06005"
     CDS             330..965
                     /locus_tag="EYO68_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HIC07395.1"
                     /translation="MLLSINPINPQARLIQQVVDVLNNDGVIIYPTDTVYGLGCSIFS
                     KKAMKRLQQIKNSNDHSGMNPKKPLTFICANQTEVQEYTQGIPTQVFKMLRRKLPGPY
                     TFVFKAAKIVPKMMLTPRSTVGLRWPNHPITNEIVKTFGHPILSISLRISEDELYDDP
                     HELHEKYKKQVDLIVDGGTIFAENSTIIDFSHGEVEIVRMGKGPVDWLEEV"
     gene            complement(996..1793)
                     /locus_tag="EYO68_06010"
     CDS             complement(996..1793)
                     /locus_tag="EYO68_06010"
                     /inference="COORDINATES: protein motif:HMM:PF07963.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC07396.1"
                     /translation="MMRIFTSIPNNARINNSGFSLLELLISVVLLGLVMSLLYGAFFQ
                     ISNSSIQVKSTLNTRQELRLLMKIVLDDLQSVQYLKHFSSAVQSKSLRRETGLIAERK
                     LGPENTETGELEEVSSIYFHTSIKSRFYPDEKDHDPELHEVGYSLEENPDTKIWEFIR
                     REDFYLDDNLREGGKSYVLSEAVTKFELDLLESETALAGGGFKENWTTVWNSEEGVCI
                     DKTAADNFCLPRAVKLTMALRGDDGNTVSDSQVINLCVPPCNPEIFE"
     gene            complement(1762..2169)
                     /locus_tag="EYO68_06015"
     CDS             complement(1762..2169)
                     /locus_tag="EYO68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIC07397.1"
                     /translation="MENVGDKSGSIRKRGFTILEVLVSLSILVFILMALYQSYSTSIF
                     VLSSTTNLWKAMSHAQNELLKSERATISPPVSVNQGEFELDDPMSGFRWKKLVRDTTP
                     LPGIMVRQVNYQLLWNEGKSEFTYDADIYVNPQ"
     gene            complement(2179..2748)
                     /locus_tag="EYO68_06020"
     CDS             complement(2179..2748)
                     /locus_tag="EYO68_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07398.1"
                     /translation="MQISIQAIYKRFSYRASGVSSQRLKGFTFLEILVVLTLGILLMG
                     MVVPQFFALFSKAHESEFKHLSSVLKMLRNDAVLKSTAYCLLFDLKTQQMMTTEEDES
                     GKCGKDILDNPKVLKPHDFPEDLSLSTAKLVGDEFSSSGTASDLLEVHINRSGFVSPF
                     FLVYSLHDSSKSWIIESKGIMGKLQLREQ"
     gene            complement(2720..3193)
                     /gene="gspG"
                     /locus_tag="EYO68_06025"
     CDS             complement(2720..3193)
                     /gene="gspG"
                     /locus_tag="EYO68_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HIC07399.1"
                     /translation="MKNKRKQIIVNATLDKSKKRSRGFSFIEVMVVIIILGLLSSIVG
                     VYLFDSAEKAKVDATKTQIRGLETALDLYRLHNSRYPTSEQGLKALLDKPEVGMIPKN
                     WNGPYLRGNNLPEDSWDSPFRYVSVNGKNYEIISLGADGVDGGTDLDADINSSNL"
     gene            3268..3649
                     /gene="rnpB"
                     /locus_tag="EYO68_06030"
     ncRNA           3268..3649
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="EYO68_06030"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3744..4541)
                     /locus_tag="EYO68_06035"
     CDS             complement(3744..4541)
                     /locus_tag="EYO68_06035"
                     /inference="COORDINATES: protein motif:HMM:PF13413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07400.1"
                     /translation="MTSQANNPQVEENYYQILEVPKTASLVEIRDAYEGKLEETQLDA
                     FAAYSLFPEDETEETLLNFSQAFVTLANPVARAKYDDELQQGMVSAEMQLVTEKEHLT
                     KIKKLFSKKSVREKTKTVPPAKKRPEKEEAGKEKEIKKSVAREISAESQEERREHYHT
                     LAVKNELAAENLEDFYSTFRTHGGKLSYNGTVLQQIRKIKSVTLEELSQITCIRVTYL
                     QAIEDENFSKFTSEIYLKGYLLCYVEALQLPKEQVLTDYILLYKKSF"
     gene            complement(4538..5479)
                     /locus_tag="EYO68_06040"
     CDS             complement(4538..5479)
                     /locus_tag="EYO68_06040"
                     /inference="COORDINATES: protein motif:HMM:PF01656.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HIC07401.1"
                     /translation="MEKKIWAIGGGKGGVGKSYVAGNLGILLAQKGHNVVLADLDLGG
                     ANLHTWLGVSNPIKGITEFIERDIPDIKKLLIPTDMSGLRLISGAKDGVEIANMKHTQ
                     KRRFVTALRQLDADYIVIDLGAGTAYNTVDFFLLADSQIIIVIPEPTSIENAYRFLKN
                     SFYRQILHNSEKFRMKSTIKNILRKDNPFNINTPKQLIAYMNQLGGNAAVFIEEQQQL
                     FMPSIILNQVRSEKEKKIGVAMEKACLKYFNLNVKISGYLDFDETVRQSVIDREPLAK
                     NSLESVPVQQLSIICEKLLQEEKERTKKNNLLSQLAL"
     gene            5797..6180
                     /locus_tag="EYO68_06045"
     CDS             5797..6180
                     /locus_tag="EYO68_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07402.1"
                     /translation="MRYFIFYVSVIIFLHACSSTTPDFPQQSFRSRLSGGDRHMGWSL
                     NYFDSWQNGLQPRYLQLAEQHTIAAIKMFAHLESDTSPRISEFYVVRERRTRSCRLLA
                     EIQFAAGNHGYKLSSRTPDGCVYFY"
     gene            complement(6218..7783)
                     /locus_tag="EYO68_06050"
     CDS             complement(6218..7783)
                     /locus_tag="EYO68_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07403.1"
                     /translation="MWNLIKLLFGIVFIISVISMFQISWEIGLSVFVLITATFCIWIV
                     LKKITKVVFYPDFVLSSEPFRNSVTSGGNEEILADQDTSASGNLIPGVDESIFEQFRE
                     KLNLHPPSESTGDSAISSEKNANSGLVAEAYEKANSTNAPDTFSEELEDVEQVKVTLS
                     KDAKNLQEEQDEGNAKDTVEEQSDDAAEEQNKKVAEKQETKYREKEKTDRKLGTGEEA
                     LEILSRKHQEIRRQTRDIAAEPQEEFDEDLFADELIPIPGGETLAEPEEREFSDEDLF
                     APAPATDNLEDEDGLVSSLRKTTPRGEKNAEAEGLLKLATTSCEAGRMEEAGASLKSY
                     LDLLKELGQKPSSDVLHLAEKLGIPLDSTLTAELALKTDETKTETEKEPEKNILDEPE
                     QTNYESVMDGIVKTLEEKDSYEEALPLLKDLLNYNRQRVNISAMDPLYERIERAHSTM
                     ENDEELVATYKEHLAIKQQLDDIEGELHLLDLISAHYTNIGDLKASKRYQSEILRVKD
                     GLEYKQAMEEEVQ"
     gene            complement(7857..8816)
                     /locus_tag="EYO68_06055"
     CDS             complement(7857..8816)
                     /locus_tag="EYO68_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIC07404.1"
                     /translation="MKKISVNRQQNDEKIEKDQRCGFIGLIGRPNVGKSTLLNQLVGQ
                     KVSITAHKAQTTRNRILGIRTVKNTQMVFIDTPGIHPAKKLLNRKIVAYATETLRETD
                     LNLWLIEPLPETSLKKDGLSVLHREDQEILKMLSGKEKRTVLVLNKIDTILQEQALVS
                     MEKLAKLGDFAEIVPISALKSTNVEHLVETLKKYLSIHPFYFENNQVTDVSERFLASE
                     FVREELFMRLQQEIPYSVAVVVEQFEEDQKCIKIACNICVERDSQKGIIIGKKGQMLK
                     TIGIAARKKIERLLGNKVHLALHVKVLKHWSSNAQHLRNLGFN"
     gene            complement(8813..10006)
                     /locus_tag="EYO68_06060"
     CDS             complement(8813..10006)
                     /locus_tag="EYO68_06060"
                     /inference="COORDINATES: protein motif:HMM:PF02541.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07405.1"
                     /translation="MSTFTCCRAPKAVPDLSGNMSNMLKEKCKKWEQKYETVSDDLNF
                     ITFFQEMSPKTFAAIEIGSNAVRMIVGELRSSCRLCVLERWSAHLRLGDSVFESGEIS
                     EQLFQQLLQTIQGLLSKVHKYSDLKLSITATSAMRSAKNNAETAARIESIVGYPLKIL
                     SGTEECQCLADGVKSFLPPSMVLDYPLKQTFLADLGGGSLEISLLKSGKSPSCKNGDY
                     QYSIDIGTLRLRKMVNPENELKSVITEKLLQLDEVLSANPQNRTNLILTGGNAKTFAR
                     LYPQVSANLRPENSSTVEAKNWLSMNWTEFECTEQIFQSQSADEQIEQWKLRPQQTEV
                     FNSALAVFRAIGSKLRTEQLCIPFFGLKESLLLSLVSVVTEKPMEDFTLVLIYGPPKK
                     YKIGA"
     gene            complement(9888..10292)
                     /locus_tag="EYO68_06065"
     CDS             complement(9888..10292)
                     /locus_tag="EYO68_06065"
                     /inference="COORDINATES: protein motif:HMM:PF11969.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIC07406.1"
                     /translation="MASIFTKIIQGEIPCEKIIETDSEIAFLDIMPCAAGHTLVIPKL
                     EVERLEDLPAEQALSLMRTMQQVAKAVSTAFDGIDYNLILNNGLNAGQEIAHVHFHVL
                     PRAKGSPGPFREHVQYAEGEMQEVGAKIRNCL"
     gene            10263..13322
                     /locus_tag="EYO68_06070"
     CDS             10263..13322
                     /locus_tag="EYO68_06070"
                     /inference="COORDINATES: protein motif:HMM:PF01103.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07407.1"
                     /translation="MNNFGKDRSHFGVSALSLKVSGSRLEVRSLLYFVFRFPLLFLVV
                     HGVLIVISTQLLSAESHFKTQAPHYKIDVSYDHDKTLLVGKMQVRFTRNAYPTHELLF
                     SLPGNRFNYPDERGTRKHKIVPVFSLRRFQDNLEDPKTPTGFSTGSLKINSVSAFTQN
                     QSVEKHPLKYSLEPNPDLEVGYSTSNGLLRILLPKNLPDTKNFPGESTVLIEFSTNFP
                     EHAQEGAVNGMLLTVNWHPKLLTWNEKPGLNEKKWETTEDNPSPATFEVTWKAVQAGT
                     LITTPGHQKLLAGQVVTLSVTKRTIKYFPLIFSRVHQQFSGNEGRAIVVKNTSTAAAK
                     TSYQLTSFYLEGHERRAELLHNWSASFLSFMHSRYGLKPPWESIRIVAVEAEYEQVDV
                     LNNLILVPMPNYKRSDFLDRQALGFLTRRLAQLWFGELIWSNQDTQQWLNLGVPAFFG
                     LRFFQHNFGADAGIFDSLDWLNPRYRDHFFEKMANSVSPKLRYPILSSFRKNPDSQKY
                     LQTLTYKTAMVLSMLEYTLGDKAFKKGIRYFAQNYQQNVIELEEFQQAMEKFNYHQLR
                     TPPLPSGSPYNMDGNGSLEWFFSQWFRTVQTLDYSFGDSTTRTLPNGLYETEVNVNKI
                     GLAQMPLVVSLITKDGKQIRRLVPGIKQQETVVFQTAGFPDKVSLDPEERLLETSRIN
                     NHSYIFYRVRFGFDWKKQREHLVLLVPGFGNNALDGNSVGVGIRYRFDDYRIYAIPGY
                     GSKNKRGLYIFNLDREHLGLHGLEAGVSAREYGGVRSQGIRATYKPSNNPGELEYKFH
                     SSFSREILFSARNNPDNSDVIETGESNTFLLEHTGAVSPIDSYRINWNIWNEQPSLEM
                     ESDFSYVRGQAKLGQILRVGHRKWFEFDIIHATTSGKSPLQKKFQLGSPAVLRGYPQQ
                     TNLSDDHLLASRLNFKFPLITKPLWGMLSAFKIQGTVFYDQGKIWSENISYEKAKHRE
                     NAGMGIEWTLDTASLFQVPLKIEVAFPLNDPDYKKPQFIFLGVLTGS"
     gene            13643..14686
                     /locus_tag="EYO68_06075"
     CDS             13643..14686
                     /locus_tag="EYO68_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07408.1"
                     /translation="MLSRKPKKFSLLDELGVKRSSSVRSKNSSDSPSVSIRKEGLSLL
                     SPKPKRKKSAADAATSILDELGVKRSSSVRSKKISPVGIQKKAEQKKETTEELESWER
                     IVLKNHEIQIPHTKTKILNKSTTKKTEQKKFEKIDSSAQKTAELPEVKFGFHEKFVLL
                     SMLVVMGVASLAIIGGHLKLLDSAIFYQLVGGNVHQLEFAGEFEARLVENGYNRLPVF
                     VVEGSIRNTFNESDQIKKIQLKAFAFDADQQLIANHFTYAGTVLSDEQLESLSPMDIK
                     ALRHSSEFGIQDSTALTNDKNDLLNTSLPQAPLIPFQVVFIKSVPTIKKTSLQIVSYV
                     RNNKVVYVHAPNL"
     gene            14763..15899
                     /locus_tag="EYO68_06080"
     CDS             14763..15899
                     /locus_tag="EYO68_06080"
                     /inference="COORDINATES: protein motif:HMM:PF04339.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIC07409.1"
                     /translation="MKFVQSKFEPKIGAVSSKQWSTLKGTEFPFSEHNYLNALEESSC
                     VGEESGWLPCHLTLWEKKQLQGAVCLYEKYNGYGEYIFDWGWANAYEKHGLNYYPKLV
                     SAIPFTPATGLKLLVHPEADTSNVRKKLLEEALLCMRSRNCSSLHFLFITAEELPVFE
                     SMGFLIRHSFQFHWKNNFYQDFDEFLGSLKRKRRKEIIRERRQIQQQMIKVEVLEGEA
                     ILVEHINYMYEFYLSTSEKKWGRPYLSKDFFSRIHQTMCNKLVLFLAYVDGDCVAGTI
                     NFKNGKCLYGRYWGCKGNFRSLHFELCYYQPIEYAIRKGISLFEAGAQGEHKIQRGFL
                     PELTYSAHWLEHQGFRSSISKFLEEEKAAIDQGIEEFTPHSPYR"
     gene            complement(15908..16603)
                     /locus_tag="EYO68_06085"
     CDS             complement(15908..16603)
                     /locus_tag="EYO68_06085"
                     /inference="COORDINATES: protein motif:HMM:PF07508.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIC07410.1"
                     /translation="MSIYGYTRLNNHRPTPDKLDTRNQAGIIRKWSEKKELKLKKIFR
                     DIESNSASLELPNLKKLITLIVQDKVTVLIVARLDRLTRSIRLYKQLLNLFEKHKIRF
                     VSVAEGLDSKTKSGKMVLDALSIMALWDAKSIPDRTREMIERKREIGERVGHAPFGYT
                     YRNKRLAPVEKELAIAKLIREKREDENLSYHKIARFLNSQRLRSKRGGRWYAETIKGI
                     CKNSLYKRTSNIK"
     gene            complement(16677..17384)
                     /locus_tag="EYO68_06090"
     CDS             complement(16677..17384)
                     /locus_tag="EYO68_06090"
                     /inference="COORDINATES: protein motif:HMM:PF07729.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HIC07411.1"
                     /translation="MLLKAVRSQRRFEQVAEQISKLIQKGKLKPGMRLPPERELALQL
                     EVSRPTVREAMIALEISGLVEVRVGSGIYVLDTSSPNLKMCKNSVDIGPGPLELIEAR
                     KEIEGAIAELAAQRIDTGCLEQLEQAIDKMVHENKIGADGDREFHLLVAKAAGNSVLE
                     TITVFLWDQQQNSPMWTKLIERMKEKKLHTTILDDHQCLLDSLRQKDSKGAKAIMRNH
                     LNHARDIYFDLMGEEEN"
     gene            17713..19194
                     /locus_tag="EYO68_06095"
     CDS             17713..19194
                     /locus_tag="EYO68_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC07412.1"
                     /translation="MSFEKNIRGEFVRILGKEWVKDDQVTLYTYRCDGLALYTAPPMG
                     VLFPGTVQELSSVIKLLNQHKISFVARGAGTGLSGGAVPQEGSVIIEMARFKEVHEVD
                     WVNRTITVGPGVINLHITENVHSHGYHYAPDPSSQKACTIGGNVAENSGGPHTLKYGV
                     TVNHVLGVEMVLPDGELVTLGGKHLGMPGPDLLGLVTGSEGTFGIISKIICRLTPNPE
                     KAVTLLGIYTSVRNACESVSSIIRHGIIPAAMEMLDKITISAVEKALKPGFPLDAEAV
                     LIVELDGLADGLDAEAEIVEKLLKETGATKVNRAQDEAERAKIWRARKEAFGAIGQIS
                     PSYYVQDGVIPRSKLPEVLDEITAIGKKHGLTVANVFHAGDGNLHPLILYDYENPEEV
                     EKSHKIGEEILSACIRYGGTLSGEHGIGIEKAEWMSDLYSENSLANMQNVRVFFNPEN
                     LLNPGKIFPHPGRCAESKAGTPPPNLSAVNKTKMVAAKSGIAV"
     gene            19191..20399
                     /locus_tag="EYO68_06100"
     CDS             19191..20399
                     /locus_tag="EYO68_06100"
                     /inference="COORDINATES: protein motif:HMM:PF01565.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC07413.1"
                     /translation="MSKIEPGSLEELQNWVNSRIETQVPFRICGNGSRFVSTISGNQK
                     DIPGDILSLKKLDGTRFFDPDDMVVGVEAGMSIRTLQGMLAEENMVLPVNPWFPDSCV
                     GSVVACNDYGPNRMDMGGLRDCIIGIEYINGKGELVQAGGKVVKNVSGYDLTRMMLGS
                     QGGLGVITAVNFKVMPRPVEPHGMFGIFRDEAWLSQLKELRKLRIPLNWIQALSTSDS
                     SWVLGMGYSGNKLNRNRIEGEIREVFGKTLNIQPDGKSYFGQIFTPGEQRFTGFLPSI
                     RDAWKMEESHFHVFATLPTEEIFTFPFETFRKANFKIAVHPTGGDINFMHKSTERKEQ
                     LQHLEKIKSALIHADSRLRWVSSTPKCSFMDLGKFGVASGYSLVKRLKKHLDPAGVFF
                     APYYDLEFDE"
     gene            20396..21808
                     /locus_tag="EYO68_06105"
     CDS             20396..21808
                     /locus_tag="EYO68_06105"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02754.14,HMM:PF13183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIC07414.1"
                     /translation="MSINFSNKTAVSTKELFRQAEFDNILKCVHCGLCLESCPTYREL
                     EDEKDSPRGRLYLMRGLWEGELELEQSVIDPLSRCLDCRACESACPSGVPYGELLEKT
                     RGIILENTPQSLKERFLRNLLLKGLFRYTSIMTAASRILKIYAATGLPKFITKTFIGK
                     LLPKSFVFQQHLLPNCSGESFKRKYADQILSPVFPEKTLHTTQNRKSKIRVGLFSGCI
                     MDVSESAIHESTLTLLRHFGCEVVVPGNQGCCGALHVHSGDRKTAREFALKNLAAFEP
                     RHFDAIITNAAGCGAQLKEYHHLFSDETNGSEQDWVGFENKVIDILEFLSRFPELLES
                     LSWREDQDIVLYDAPCHLMHAQKVDENPRQLLNSLPGVKLVPLKESNWCCGSAGIYNL
                     VQPDLSRAVLKRKTESVRQTLTENPKAKTIITGNPGCLYQIRAGIRSENIDLRILHPA
                     VYLVERLKKLGTRDIPQIPI"
     gene            complement(21843..22154)
                     /locus_tag="EYO68_06110"
     CDS             complement(21843..22154)
                     /locus_tag="EYO68_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine methyltransferase"
                     /protein_id="HIC07415.1"
                     /translation="MKSPIYQQIHEIIRLIPVGKVATYGQIADIVGGCTARMVGYAAS
                     AIPFDSDIPWQRVINFQGGISTRSGGSGELLQQELLEAEGIQFDQNGRTNLELYRWKG
                     E"
     gene            complement(22161..23354)
                     /locus_tag="EYO68_06115"
     CDS             complement(22161..23354)
                     /locus_tag="EYO68_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07416.1"
                     /translation="MNEKTKDFRPFICIDNAGIYPFKDASILLINNAKRTQQTDHNGK
                     TISDLIKGKKALSWQTARNSFPGDVRRIDPAELLTFWNEKKLNGHFQQKGDTYTETLT
                     PFLKIEDDGSFKMSAKLHKIIVDLEKDFNGRMLLIFRDEALHLVLESTAKNVFIKSGN
                     VSANLTSQFPENEPVWSIPLEAKFFKLLKNKNPAKLEFQVISNRLHVKNHSTSKEQTL
                     DLKPAISSFNFASRWNPPEEGPLIETVVTKDVMAKLRDISVNSSYKRLGIVGNSGQIK
                     VALFGNEPDDFLFLGNEIGTCTTEFQVVSHPMPMLEDDFIISIYENQILRLRSLTEDY
                     EWMTFFEKDFSILDASSYAEKSGSSLPAKSQEHLQLVQNKLKVASAENSVTETKTRDL
                     AKSVS"
BASE COUNT         6657 a         4890 c         4734 g         7167 t
ORIGIN      
        1 tcagggctga tttaccgttt tgaccttgca aaaggagatg acggcattgc accaacactg
       61 tttattaatt atcctttgtc attaggtaca cttgggagtt agaaacgctt tgtcagagtt
      121 tcaatgcttc tgaaacatcc aggggaacac aggtgcagaa actggttgtt gtgccagcaa
      181 tttcttttat tgctcagaat tttaagcatt ccaagatatg tttcttccgg ctggaaatgt
      241 ttcaccagcc aactaatact tcctttacag cagacaattt gttaagtctt tgagtttgtt
      301 gcactgactt agctttcagt aaatttcaca tgcttttgag tattaatcca ataaatcctc
      361 aggctcgact cattcaacaa gtcgtggatg ttttgaataa cgatggagtc atcatttacc
      421 ccactgatac agtttacggg ctgggctgca gcattttcag caaaaaagct atgaagcgcc
      481 tgcagcaaat taaaaacagt aacgaccaca gcggtatgaa tcctaaaaaa cctttgacat
      541 tcatctgcgc taatcaaact gaagtccagg aatacactca gggaattcca acacaagttt
      601 ttaaaatgct tcgcaggaaa ttaccaggtc cctacacttt tgtctttaag gcagccaaaa
      661 ttgtacccaa aatgatgctc acaccaagaa gtactgttgg tttgcgctgg ccgaatcatc
      721 ctattaccaa cgaaatagtt aaaacctttg gacacccgat tctcagcatc agtttgagaa
      781 tatcagaaga tgaactgtac gacgatccac acgaattaca tgaaaaatac aaaaaacaag
      841 tggatttaat tgtagacggc gggactattt ttgccgaaaa ttcgaccatt atagatttca
      901 gtcacggaga agtagagatt gtccggatgg gtaaagggcc tgttgactgg ctggaagagg
      961 tttaaccctt tgtagtgtaa aattttcaat tcaggctatt caaatatttc cggattacaa
     1021 ggaggaacac atagatttat gacctgcgaa tccgaaactg tgtttccatc atcacccctg
     1081 agagccatag ttaattttac tgcacggggg agacagaaat tatctgcagc agttttatca
     1141 atacatactc cttcctcaga attccagact gttgtccaat tttccttgaa tccgcctcct
     1201 gcaagagcag tctcactttc aagtaaatcc agctcaaact ttgttaccgc ttccgacaaa
     1261 acatagcttt ttcctccctc tctgagattg tcgtcaagat aaaaatcttc acggcggata
     1321 aactcccaaa tttttgtatc gggattttcc tccagagaat agccgacttc atgcagttca
     1381 gggtcatgat ctttctcatc cgggtagaaa cgtgacttga ttgaagtatg aaaataaatg
     1441 ctgcttactt cttcaagttc tcccgtttca gtattttccg gacccagttt tctttctgca
     1501 atcaatccag tctctcgtcg cagagatttg ctttgaacgg cactggagaa gtgttttaaa
     1561 tattgtacgg actgcaaatc atctagaact attttcatca gcaaccgcaa ttcctggcgg
     1621 gtgttcaaag ttgattttac ctggatagag gaatttgata tttggaaaaa cgcaccgtaa
     1681 agtaaggaca tcaccagtcc gagcaggaca acagaaatta ataactccaa cagggaaaat
     1741 ccagaattgt tgattcgggc gttattgggg attgacgtaa atatccgcat cataagtaaa
     1801 ttcgcttttt ccctcgttcc agagtaattg atagttgacc tgccgtacca ttattccggg
     1861 aagaggtgtg gtgtcccgga caagtttttt ccagcggaaa ccactcattg gatcatcaag
     1921 ttcaaactct ccctggttga ctgaaacagg aggtgaaatg gttgcacgtt ctgatttcaa
     1981 taattcattc tgggcatgtg acatggcttt ccacaagttt gttgtggaag agagaacaaa
     2041 aatggaagtg gaataagact ggtaaagtgc catcaggata aacaccagga tcgagaggga
     2101 aactagaacc tccaagattg taaaacctct tttcctgatt gatcctgatt tgtctccaac
     2161 attttccatg actgaacttc actgttcacg taactgcagt tttcccatga ttcccttact
     2221 ttcaattatc catgatttgg aagaatcatg taaagagtac accaggaaaa aagggctgac
     2281 aaatcctgat ctgttgatat ggacctccag caaatcagaa gcagttccgg aagaagaaaa
     2341 ttcatcaccg acgagttttg cagtacttaa gctgagatct tccggaaaat catgaggttt
     2401 cagaactttt ggattatcca aaatatcttt gccgcatttt ccagactcat cctcttccgt
     2461 ggtcatcatt tgctgtgtct tcaaatcaaa gagcaggcaa taagcagtgc ttttgagaac
     2521 agcatcgttg cgcagcattt tcagcacgct gcttagatgt ttaaattcgg attcatgtgc
     2581 tttcgaaaac agggcaaaaa actgaggtac aaccattccc atcagcagaa tccctaatgt
     2641 caataccacc aaaatttcca gaaaagtgaa gcctttgagt ctttgactgc tgactcctga
     2701 ggcacggtag ctgaatcttt tatagattgc ttgaattgat atctgcatcc aaatccgttc
     2761 ctccatctac accatccgca ccaagtgaaa taatttcata atttttaccg ttcacactga
     2821 cataacggaa tgggctgtcc caactgtctt ccggcaaatt atttcctctt aaatagggtc
     2881 cgttccagtt ttttggaatc attcccactt ccggtttgtc caacagtgct ttaagtccct
     2941 gttctgatgt aggatagcgg gaattgtgca ggcgataaag atccagtgca gtttccaaac
     3001 ccctgatttg ggtttttgtt gcatcgactt ttgctttttc tgcagaatca aaaaggtaca
     3061 ctcccacaat actggaaagc agccccagga taattatcac aaccattacc tcaatgaaac
     3121 tgaatccgcg gctgcgcttt ttgcttttat ccagagtagc atttacaata atttgtttcc
     3181 gtttattttt catctgagtt caaatctgag tgtgttaaaa gaagttcgct tgctcttatc
     3241 cacaatgatg ctagtttagt aagcatcaga gccggatacg tggccgcccg ctttcctgat
     3301 tattttcgaa tccggggggc tggaggaaag tccgggcaac atagggcaag ggtgctggct
     3361 aacggccagg cggcgcgagc cgatggaaag tgcaacagaa aacataccgc cgatggtttc
     3421 tccggaaaca caggtaaggg tgaaatggca gggtaagagc ctaccgcagt cttggtgaca
     3481 agactggcag tgtaaacccc accagttgca agtccaaata gaaccgctat caggtggccc
     3541 gcctggcggt cgggtaggac gctagaggcg gccggtaacg gcaatcctag ataaatggtc
     3601 acgactcaca tctgtgagaa cagaacccgg cttatttccg actctgactt tttccaaact
     3661 gacacaagct ttcagttcaa cagccctacg atgctgtatt gaattgtaca gtaccagcat
     3721 aaaacatttc aactcttttt agactaaaat gattttttat agagaagaat ataatctgtt
     3781 aaaacttgct ccttgggcaa ttgcagggcc tcaacgtagc aaagcaaata gccctttaaa
     3841 taaatctcag atgtaaattt ggagaaattt tcgtcttcaa tcgcttggag ataggttaca
     3901 cgaatacagg tgatttgtga gagttcttcc agagtgactg attttatttt tctaatttgc
     3961 tgtaatacag taccgttgta tgatagttta cccccatggg ttctgaaagt ggagtaaaaa
     4021 tcttctagat tttctgcagc aagttcattt tttactgcca gggtatggta atgttcacgg
     4081 cgttcttcct ggctttctgc gctaatttct ctggcaacac ttttcttgat ttctttctct
     4141 ttcccagctt cttctttttc tgggcgtttt ttggcgggag gaactgtttt agttttttcc
     4201 cttactgatt ttttggagaa cagtttttta attttagtaa gatgctcttt ctcagtgaca
     4261 agctgcatct ctgctgaaac cataccctgt tgtaattcat catcatattt tgcccgggca
     4321 acaggattag ccagagtcac aaaagcctga ctgaaattca gtaatgtttc ttcagtctca
     4381 tcttccggaa acaaggaata tgcagcaaag gcatctaatt gagtttcctc cagtttaccc
     4441 tcgtaagcat cacggatctc taccaatgaa gcagtttttg gtacttcaag aatctgataa
     4501 taattttctt caacctgtgg attatttgcc tgagaagtca taatgctagc tgtgacaaca
     4561 gattattttt tttagttctt tccttctctt cctgtaataa tttttcgcag attattgaca
     4621 gttgctggac tggcacgctc tccagtgaat tctttgctaa aggttcacga tctatgaccg
     4681 actgcctgac tgtttcatcg aaatctaaat atcccgaaat tttcacattc aggttaaaat
     4741 attttaaaca tgctttttcc attgccacac caatcttttt ttccttttcg gaacgtacct
     4801 gattcaggat aatacttggc atgaacaact gctgctgttc ctctataaaa accgccgcat
     4861 tgcctcccag ttgattcatg taagcaatga gttgtttcgg ggtatttatg ttgaatgggt
     4921 tgtcctttcg caaaatattt tttatcgttg atttcattct aaatttttca ctgttgtgaa
     4981 gaatttgacg ataaaatgaa ttctttaaaa aacgataggc attttcgatt gatgtcggtt
     5041 ccggaattac aataattatt tgcgagtctg ccagcagaaa aaaatcaact gtgttgtatg
     5101 cagtgcctgc acctaaatca atcacaatat aatctgcatc caattgtctc agggcagtta
     5161 cgaaacgacg tttttgagtg tgtttcatat ttgctatttc gaccccgtct ttggctccgc
     5221 tgatcagacg caatcccgac atatcggtgg gtattaataa ctttttgatg tcaggtatgt
     5281 ctctttcaat gaattctgta atccctttaa tgggattgct cacaccaagc caggtgtgca
     5341 ggtttgctcc acccaaatcc aagtcagcta gaactacatt gtgacccttc tgagccagca
     5401 gaatgcccaa attgccggca acataacttt tgccaacgcc tcctttacct ccaccaatag
     5461 cccaaatttt cttttccatt ttagccagtt aaagtctgtc gaaatgagtt gctttttttt
     5521 gtcgttctta caaaactgca aatagttggc caattttaat ttgtaaagat taacagttaa
     5581 gcataacatc tttgttttta gtgacttaag ttgaaggcaa aagcaaaatt atgcaagccg
     5641 gatcgtgaag ttccttactt ttttaaaggt ttttggcagg aggccgatac atattacagg
     5701 cgggttacta tactctttta aagaggaatt gaaacaagaa atccaaaaac ttcagtaatt
     5761 ttacataaga cattcagcag gccaaaacaa atgtttatgc gctattttat tttttatgtt
     5821 tcagtcatta tttttttgca cgcttgttca agtactactc ctgattttcc acaacaatcc
     5881 tttcgcagcc ggttaagcgg aggagatcgt catatgggat ggagtctgaa ttattttgac
     5941 agctggcaga atggacttca gccccgctac ctgcagttgg cagaacagca cactattgcg
     6001 gcaataaaaa tgtttgccca ccttgaatct gacacatcgc cgcgaatcag tgagttttac
     6061 gtagtacgtg aaagacgtac acgcagttgc agactgctgg ctgaaataca gtttgcagca
     6121 ggaaaccacg gctacaagct aagttcccgt actcccgacg ggtgcgtata tttttactga
     6181 ttttatttta gtcggttgta tccactgaat cagtacttta ctgtacttct tcttccattg
     6241 cttgcttgta ttctaatccg tcttttactc ttaaaatttc tgactgatag cgttttgatg
     6301 ctttcagatc tccaatattt gtatagtggg cactgatcaa atcaagcaag tgcaactcac
     6361 cttcaatgtc atccaactgt tgtttgattg caagatgttc tttgtaggta gctactaatt
     6421 cttcatcatt ttccattgtt gaatgtgcac gctcaattct ttcgtaaagg ggatccattg
     6481 ctgaaatatt cactcgctgc cgattgtagt tcagcagatc ctttagcagc ggcaaggctt
     6541 cttcatatga atctttttct tcaagagtct tcactattcc atccatgaca ctttcataat
     6601 tggtctgctc tggttcatcc agaatatttt tttccggttc tttttcagtc tctgtttttg
     6661 tttcatcagt tttcaaagca agttccgcag tcaaagttga atcaagtgga atcccaagct
     6721 tttcagctaa atgcagcaca tctgaacttg gcttttgacc aagctctttg agcagatcca
     6781 gataactttt aagactggca ccggcttctt ccattctgcc tgcttcgcat gatgttgttg
     6841 caagtttcag cagtccttca gcttcagcat ttttttctcc ccgaggagtt gttttccgta
     6901 aagaagaaac caaaccatcc tcatcttcca gattgtctgt tgccggtgcc ggggcaaata
     6961 agtcttcatc actgaactct ctttcttctg gttcagcaag ggtttctcct ccaggaatcg
     7021 gaatcagttc atctgcaaaa agatcctcat cgaattcttc ctggggctct gcagcaatat
     7081 ctcttgtttg tcttcttatt tcctgatgtt ttcgagaaag aatttccaga gcttcttctc
     7141 cagtacctag tttacggtct gtcttttctt tttccctgta ttttgtttcc tgtttttcag
     7201 caactttttt gttttgttct tccgctgcat cgtcactttg ctcctcaact gtgtcttttg
     7261 cgttaccttc atcctgttct tcttgaagat tttttgcatc tttgctcaaa gtaactttca
     7321 cttgttctac atcctccagt tcttcagaaa aagtatcagg agcatttgtt gaattggctt
     7381 tttcatatgc ttctgcaacc aagcctgagt ttgcattttt ttcagaggat atagctgagt
     7441 ctccggtaga ttcggaagga gggtgtaaat ttaatttttc tctgaattgt tcaaaaatag
     7501 attcgtcaac accaggaatt agatttcctg atgcagaagt atcttgatca gctaaaattt
     7561 cttcattgcc gccagaagtt actgaattac gaaacggttc tgaagaaaga acaaaatcag
     7621 gataaaatac aacttttgta atttttttca gtacaatcca gatacaaaat gtagcagtga
     7681 tcaaaacaaa aacacttaga ccaatttccc acgatatttg aaacattgaa ataactgaaa
     7741 taatgaaaac tatgccaaac agtaatttta ttaaattcca catatctttt gatattgaaa
     7801 tttttgcctg aaatcaggaa ctgaaaatat tagttttgac tcttgttacc tgcaagttag
     7861 ttaaatccaa gattgcgcaa atgctgtgca ttgctggacc aatgttttaa aactttcaca
     7921 tgcagtgcta aatgaacctt gtttccaagt aatcgctcga tttttttccg ggcggcaatc
     7981 ccgattgttt tcaacatttg accttttttt ccaatgatga tccccttctg tgaatctcgc
     8041 tccacacaaa tattgcaggc aatcttaata catttttgat cctcttcaaa ttgttccact
     8101 acaactgcaa ccgaataagg aatttcctgc tgcaggcgca taaacagttc ctcgcgtaca
     8161 aattcactgg caaggaagcg ttctgacaca tcggtaacct ggttgttttc aaagtaaaat
     8221 ggatggatag aaagatattt tttcagggtt tcaaccaggt gttcaacatt agttgatttc
     8281 agcgctgaga ttggaacaat ttcagcaaag tctcccaatt tcgctaattt ttccatgctg
     8341 acaagtgcct gttcctgtaa aattgtatca attttattca aaaccagcac ggtccgtttt
     8401 tcctttccgg acaacatttt tagtatttcc tgatcttcac gatgcaaaac agagagacca
     8461 tcctttttca aagaagtttc aggcaaaggt tcaattaacc acaaattcaa atctgtttcc
     8521 cgcaaagttt ctgtcgcata tgccactatt ttacggttca gcagcttttt tgcaggatga
     8581 attccaggcg tgtcaatgaa aaccatctgt gtatttttta cagtccggat accaagaatg
     8641 cggtttctgg tagtttgtgc tttgtgtgca gtgattgaaa ctttctgtcc aaccagttgg
     8701 ttcagtaaag ttgatttacc aacatttgga cggcctatta atcctataaa tccacaacgc
     8761 tgatctttct ctattttttc atcattttgt tgcctgttga cgctaatttt tttcatgcac
     8821 caattttata cttttttgga ggtccataaa tcagaaccag tgtgaaatct tccataggtt
     8881 tttccgttac cactgaaacc aggcttaaca gtagtgattc cttcaaacca aaaaaaggga
     8941 tgcacagctg ctcagttctg agtttactgc cgattgcccg aaagaccgcc agagcagaat
     9001 taaaaacttc tgtctgttga ggacgtagtt tccattgttc aatttgttca tctgctgact
     9061 ggctctgaaa aatctgttcg gtgcactcaa attcagtcca attcatactc agccagtttt
     9121 ttgcctctac cgtacttgaa ttttcaggtc gaagatttgc tgaaacttgc ggataaagac
     9181 gcgcaaatgt ttttgcgttt ccacccgtta aaatcaaatt cgttcggttc tgaggattag
     9241 cagaaagaac ctcatccaac tgtaataact tttccgtaat tactgatttc agctcatttt
     9301 caggattgac cattttccgc agccgcaatg tgccgatatc tatactgtac tgataatctc
     9361 cattcttgca gctcggactt tttcctgatt tcagtagaga tatttccaaa ctgcctccac
     9421 ctaaatccgc caaaaaagtt tgttttaacg gatagtccaa aaccatagac ggaggcaaaa
     9481 aagattttac tccatccgct aaacattgac actcttctgt tccggaaaga atttttagag
     9541 gatatccaac tattgattca atccgggcag ccgtttcagc attatttttt gctgagcgca
     9601 ttgcgcttgt ggcggttatc gataatttga ggtcggaata tttatgaacc ttacttagca
     9661 aaccctgaat tgtctgcagc aactgttgaa ataattgttc agaaatttct ccggactcaa
     9721 aaactgaatc acccaaacgc aagtgggcac tccaccgttc aagtacacat aacctgcagg
     9781 aactcctcaa ttctccgaca atcatgcgta ctgcattact gccaatttct atggcggcaa
     9841 aggtctttgg actcatctct tgaaaaaaag taataaaatt gagatcatca gagacagttt
     9901 cgtatttttg ctcccacttc ttgcatttct ccttcagcat attggacatg ttcccggaaa
     9961 ggtccgggac tgcctttggc gcgcggcagc acgtgaaagt ggacatgcgc tatctcctgt
    10021 ccggcattca aaccgttgtt gaggatcagg ttatagtcaa ttccatcgaa agctgtacta
    10081 actgcttttg ccacttgttg cattgtacgc atcaacgaca gagcctgctc tgcaggtaaa
    10141 tcttctaagc gctcaacttc aagttttggg atcaccagtg tgtgtcctgc agcgcagggc
    10201 atgatgtcta aaaatgcaat ttctgaatcc gtttcaataa ttttttcaca aggaatttca
    10261 ccttgaataa ttttggtaaa gatcgaagcc attttggtgt ttcagcttta agtttaaaag
    10321 tttcaggttc gagactcgag gtgcgcagtt tgttgtactt cgtttttcgt ttcccgttgt
    10381 tgtttttagt tgttcacgga gtgctgattg taatttcaac tcagctttta tctgcagaat
    10441 cccactttaa aacgcaggcc ccgcactaca aaatagacgt ttcttatgat cacgataaga
    10501 cacttcttgt gggaaaaatg caagtccgtt tcactcgaaa cgcttatccg actcatgaat
    10561 tactcttttc tctaccggga aaccgtttca attatccgga tgaacggggt accagaaaac
    10621 acaaaatagt acctgtcttt tcactgagac gctttcaaga taatctcgaa gatccaaaaa
    10681 ctccaactgg tttcagtacc ggaagcttaa agatcaattc cgtgagtgcg ttcactcaaa
    10741 atcaatcagt agaaaaacac ccgctgaaat attcactgga accaaatcct gacctggagg
    10801 ttggttattc aacttcaaat ggtctgctgc gaatattgtt gccaaaaaat cttccggata
    10861 caaaaaattt tcctggagag tcaactgttc tgatagagtt ttctacaaat ttccccgaac
    10921 acgcacaaga aggtgcagtc aatggaatgt tgctgacagt aaattggcat ccaaaactct
    10981 taacctggaa tgaaaaacct ggattgaatg agaaaaaatg ggaaactact gaagataatc
    11041 cgtcacctgc cacatttgag gttacctgga aagcagttca ggcgggaaca ttaatcacga
    11101 ccccgggtca tcaaaaattg ttggcggggc aagttgtaac tctttctgtt acaaagagaa
    11161 cgattaaata cttccccttg attttttcca gagttcatca acaattttcc ggaaatgaag
    11221 gaagagcaat tgtggtgaaa aatacaagca ctgcagctgc taaaacttct taccaactga
    11281 cttcatttta cttggaaggt catgaacgaa gagctgaact tctacataat tggagcgcca
    11341 gttttttgtc atttatgcat tcacgttatg gactcaaacc gccctgggaa tcaattcgga
    11401 tagttgcagt tgaagcagaa tatgagcagg tggacgtttt gaacaatttg atcctggtcc
    11461 ccatgccgaa ctacaaaagg tcagatttcc tggaccggca ggctcttgga tttttgacac
    11521 ggcgactggc gcaactttgg tttggagagt taatctggag caaccaggat actcaacagt
    11581 ggctcaattt gggggttcct gctttttttg gattgcgttt ttttcaacat aattttggtg
    11641 cagatgcagg aatttttgat agtcttgact ggctgaatcc acgttaccgg gatcattttt
    11701 tcgaaaaaat ggcaaactct gtttcaccaa aattgagata tcccattctc tcatcattcc
    11761 gaaaaaatcc tgactctcaa aaatatttgc agacacttac ctacaagact gcaatggttc
    11821 tttccatgtt ggaatacaca ctgggagaca aggcatttaa aaaaggtatc cggtactttg
    11881 cccaaaatta tcagcaaaat gtaattgaac tggaagaatt tcagcaggca atggagaagt
    11941 ttaattatca ccaactcaga acgcctccac ttccttccgg gagtccttac aatatggatg
    12001 gaaacggttc attggaatgg tttttttcac agtggttccg cactgtacaa actttggatt
    12061 acagttttgg agattcaact actcgaactc tgcccaatgg gttgtatgag actgaagtaa
    12121 atgtcaataa aattggactt gctcaaatgc cgctggtagt ttcacttatc acaaaagacg
    12181 gtaaacagat tcgcaggctg gtaccaggaa ttaaacagca ggagacagta gtctttcaaa
    12241 cggcgggttt tccggataaa gtttcacttg atccagaaga acgattgctg gaaacatcga
    12301 ggatcaataa tcattcttat attttttatc gtgttcgttt tggtttcgac tggaaaaaac
    12361 agcgtgaaca tttggtgctt ttagttcctg gatttgggaa caatgcctta gatggaaatt
    12421 ctgttggtgt aggaataaga tacaggtttg atgactatcg tatttatgca attcctggat
    12481 atggttcaaa aaataaacga ggactctaca tttttaatct cgaccgtgaa cacctgggat
    12541 tgcatggatt ggaggccgga gtaagtgccc gtgagtatgg tggagtccgt tcccagggca
    12601 tccgggccac atacaaaccc tctaataatc ccggtgaact ggaatataag ttccactcca
    12661 gtttttcccg ggagattctt ttttcggctc gcaacaatcc tgacaacagt gatgtaattg
    12721 aaaccggaga atcaaacact tttctgctgg aacatacggg tgctgtcagt cccatagact
    12781 cctaccgaat taactggaac atctggaacg aacagccttc tctggaaatg gaaagcgatt
    12841 tttcttatgt tcgcgggcaa gcaaaacttg gacaaatatt gcgcgttgga catcggaaat
    12901 ggtttgagtt cgatatcatt cacgctacta cttcgggaaa atctccgctg cagaaaaaat
    12961 ttcaactggg aagccccgcc gtattacgtg gatatcctca acaaacaaat ttgagtgacg
    13021 accatctgtt ggcttcaaga ttgaatttca agtttccctt aatcactaaa cctttgtggg
    13081 gaatgttgag tgcctttaaa atacaaggta ctgtgtttta tgaccagggt aaaatatggt
    13141 cggaaaacat ttcatatgaa aaagcaaaac accgggaaaa tgccggtatg ggtattgagt
    13201 ggacgctgga tactgcttca ctgtttcaag tacctctgaa aattgaagta gcctttccgt
    13261 tgaatgatcc tgattataaa aaaccacagt tcattttcct gggtgtcctc acaggaagtt
    13321 aaaatacata cgcctactat ttcagggtag ccttgctttt ttttatttta aatgtaaaat
    13381 cagcatccac tggacgtttt ctgtagatat gcgacaataa ttcaattctg aaattacttg
    13441 acgtggaaca tacactaaaa tactacaact cgacagtcac tagttttgta gtgttatcct
    13501 gaacataagt gagggatctt gtacggtatc agcactttgc cagatatctc ccttcggccg
    13561 agatgacgag tgaaagtcaa taatatccgt accttactat cactcatgac tcacgattaa
    13621 tacaattata aactgattta aaatgctgtc gagaaaacca aaaaaatttt ccctccttga
    13681 tgagctcgga gttaaacgat cttcctcagt cagatcaaag aattcaagtg atagtccttc
    13741 tgtgagtatc cgcaaagaag gcttgtccct gctctcacca aaaccaaaac gcaaaaagtc
    13801 tgcagctgat gctgcaactt ctatccttga tgagctcgga gttaaaagat cttcctcagt
    13861 cagatcaaaa aaaataagtc cggtagggat tcaaaaaaaa gctgaacaaa aaaaagaaac
    13921 aacagaagag ttggaatcct gggaaagaat agtcctcaag aaccatgaaa ttcaaatacc
    13981 acacactaaa acaaaaatat taaataaatc gacgactaaa aagacagaac agaaaaagtt
    14041 tgagaaaatc gattcgagtg cccaaaaaac ggctgaactg cctgaagtta aattcggttt
    14101 tcatgaaaaa tttgttttgc tttccatgtt ggtggtaatg ggtgttgcgt ctcttgccat
    14161 tatcggagga catcttaaat tactggattc agccattttt taccagttgg tcggcggtaa
    14221 tgtgcatcag ttggagtttg caggtgaatt tgaggcaaga ctagtagaaa atggatataa
    14281 tcgtctgccg gtatttgtag tcgaaggatc tattcggaac accttcaatg agtctgacca
    14341 gattaaaaaa atacaattga aggcttttgc atttgatgcc gaccaacagt tgattgcaaa
    14401 ccatttcacc tatgccggaa ctgtattgtc agacgaacag ttggaatcac tgagtcccat
    14461 ggatatcaaa gcactgcgtc attcctccga atttgggatt caggactcca ctgctttaac
    14521 gaacgacaaa aatgatttat taaatacgag ccttcctcaa gccccgttga tccctttcca
    14581 agttgttttt ataaagtctg ttccgaccat aaaaaaaaca tcacttcaaa ttgtaagtta
    14641 tgtacgtaac aataaggttg tttatgtaca tgcaccaaat ctctagtgat taacgagttc
    14701 ggaaaaaaaa ttctgaactt tcctgctggt acagccctgc tgattttcac cgttttacca
    14761 aaataaaatt tgtgcaatct aagtttgagc ccaagatagg agccgtttct tccaaacagt
    14821 ggagtacctt gaagggtacc gagtttcctt tttctgagca taattatctg aatgctctgg
    14881 aagagagcag ctgtgttggc gaagaatccg gatggttgcc ttgccatctg actttgtggg
    14941 aaaaaaagca gttacaggga gcagtttgcc tgtacgaaaa atataacgga tacggcgaat
    15001 atatttttga ctggggctgg gcaaatgctt atgaaaagca tggtctgaat tattatccaa
    15061 aattagtttc tgctatacct tttacaccgg caaccggcct gaaattactt gtgcatccgg
    15121 aagcagatac cagtaatgtc aggaagaaac tcttagaaga ggcgttgctt tgcatgcgtt
    15181 caagaaactg ttcctctctc cattttttat tcattactgc agaagagctg ccagtctttg
    15241 aatccatggg atttctaatc cggcacagtt tccagttcca ttggaaaaac aatttttacc
    15301 aggattttga tgaattcctt ggaagcttaa aacgtaaacg ccggaaggaa attatacggg
    15361 aacgcaggca aattcaacag cagatgatca aggtggaagt actggaggga gaggctattc
    15421 tggttgaaca tataaattat atgtatgaat tttatctttc tacatctgaa aaaaagtggg
    15481 gacgtccata tctttcaaaa gacttttttt cccgtatcca tcaaactatg tgcaataagc
    15541 tggtgctttt tcttgcttat gttgatggag attgtgtcgc aggaacaatc aattttaaaa
    15601 atggcaaatg cttatatggg cggtattggg gttgtaaagg aaattttagg agtttgcatt
    15661 ttgagttatg ctactatcaa ccgatagaat atgctatcag gaaaggaatt agtttgttcg
    15721 aagctggtgc tcaaggagag cacaaaatac agcgcggttt tcttccggaa ctaacctaca
    15781 gtgcacattg gctggagcat cagggatttc ggagttcaat ttcaaaattt ctggaagaag
    15841 agaaggcagc aattgatcaa ggtattgaag aattcacccc acactcacct tatcgttaaa
    15901 cagttcctta tttgatattc gaagttcgtt tgtaaagaga atttttgcaa attcctttta
    15961 tcgtttctgc ataccagcga cctccccgct tagacctcag tcgctggcta ttgaggaacc
    16021 tggctatttt gtggtaactc aaattttcat cctcccgctt ttcccggatg agtttggcaa
    16081 tagccagttc tttttctaca ggcgccagtc gtttgttccg gtacgtatat ccaaaaggag
    16141 catggcctac acgttcacca atttcccgtt ttcgttctat catttcgcgg gtccggtccg
    16201 gaattgattt tgcgtcccaa agtgccatta tactgagtgc atctaaaacc atttttccac
    16261 tttttgtttt cgaatccaaa ccttctgcta cagaaacaaa acgtatttta tgtttttcaa
    16321 ataaattaag taattgtttg taaagacgaa ttgacctcgt taaccgatcc aaacgagcaa
    16381 caatgagtac cgtgacttta tcctgtacaa tcagggtgat aagttttttt agatttggca
    16441 gttccaggct ggcactgttt gattcaatat cacggaaaat ctttttgagt ttcagttcct
    16501 ttttttcaga ccattttcga attatcccgg cctggtttcg tgtgtccaat ttatcaggag
    16561 ttggacggtg gttgtttaaa cgtgtatatc cgtaaatact catttttctt ttataaaatg
    16621 caaagccatg ttttaaattt aacaaaatac tcaattaaac ttgcctgacg agacagtcag
    16681 ttttcttctt ctcccattaa atcaaaatat atgtctcgag catgattaag gtgattcctc
    16741 atgatagctt tagccccttt ggaatctttt tgacgaagag agtccagtaa acattggtgg
    16801 tcatccagaa tggtagtgtg cagctttttc tctttcatcc gctcaatcaa ttttgtccac
    16861 atcggagaat tctgctgctg atcccagaga aatacagtta ttgtttccaa aacactgttt
    16921 ccggcagctt tggctaccag caaatgaaat tctcggtccc catcagcacc aatcttgttt
    16981 tcatgcacca ttttatctat tgcctgctcc agttgctcca ggcagcccgt gtcaatacgc
    17041 tgcgcggcaa gctctgcaat ggcaccctca atttctttgc gtgcctcaat taattcaagt
    17101 ggaccaggcc caatgtcaac agaattttta cacattttca gatttggact tgaagtgtcc
    17161 aaaacgtaaa ttccagaacc aaccctgact tcaaccaaac cagaaatttc aagtgcaatc
    17221 attgcttcac gcaccgtcgg acgacttact tcgagctgaa gtgcaagttc cctttcaggc
    17281 ggcagacgca tacccggttt gagttttcct ttttggatta atttagatat ttgttctgca
    17341 acttgttcaa aacgacgttg tgagcggact gctttcagga gcatttgatt attccaactg
    17401 atgatttatt gttttggaaa aagacaatta attttcatga cgagttcgta gggaggcgtc
    17461 ggcaaagctc atcaattcct gcaatgaatc ctctgttttc tgaggacaat ggtcaagtgg
    17521 attctgtttt atgacaaaaa taataattgg tcttacaatt ataccacttg atagagtgtt
    17581 tttaataagt tattcttaat tccagcaata tgtcccaaac aactttattg aggcctgcga
    17641 aaacatttta atgaagatcg ttttcatcac aatatattaa caaaattatt ttaattttga
    17701 ggagcgtgga acatgtcatt tgaaaaaaat atccgcggag agtttgtccg gattcttgga
    17761 aaagagtggg tcaaggatga tcaagtaaca ctttacactt accgttgcga cggtctggct
    17821 ctttacactg caccacctat gggtgttttg tttcccggaa cagttcagga actttcatcg
    17881 gtcatcaaac tgctcaatca gcacaagatc agttttgtcg cacgaggcgc cggcaccggg
    17941 ctttcgggag gcgcagttcc tcaggaagga agtgtgatta ttgaaatggc gcgtttcaag
    18001 gaagtgcatg aagtagattg ggttaaccgc acaattacag tcggtcccgg agtaattaat
    18061 ttacatatta ctgagaacgt ccactctcac ggctaccact atgctcctga cccttcttcg
    18121 caaaaagcct gcactattgg cggcaatgta gcagaaaatt ccggagggcc gcacacactc
    18181 aaatacggag taactgttaa tcatgtgctg ggtgtcgaaa tggttttgcc tgacggtgag
    18241 ttagtcacac ttggtggaaa acatcttgga atgcccggac cggatttgct gggtttggta
    18301 actggttctg aaggtacttt tggcatcatc agcaagatca tttgccgtct tactccaaac
    18361 ccggaaaaag cggtaacttt gcttggaata tacacttcag tgcgcaatgc ctgcgaatct
    18421 gtttcaagta taattcgtca cggcataatt ccggctgcaa tggaaatgct ggacaaaatc
    18481 acaatttctg ccgttgaaaa agcactcaaa ccgggcttcc ctttggatgc ggaagctgtg
    18541 ctgattgtgg aactggacgg tttggcagac ggactggatg cagaagcaga gatagttgaa
    18601 aaattactca aagaaactgg agcaactaaa gtcaatcgag ctcaagatga ggcagaacgt
    18661 gccaaaatct ggcgtgcacg taaagaagct tttggcgcaa ttggtcaaat atcaccgagc
    18721 tattatgtgc aggacggtgt aattccacgc agcaaacttc cggaagtact cgatgaaatc
    18781 acagcaattg gtaaaaaaca cggtttgact gtggccaatg tttttcatgc aggagacggc
    18841 aatttgcatc cgctgatttt gtatgattat gaaaatcctg aagaggttga aaaatcacat
    18901 aaaattggcg aagaaatatt atcggcctgt atccgttacg gcggtacgct ttccggagaa
    18961 catggaattg gtattgaaaa agccgagtgg atgtctgatc tttactcaga gaattcgctt
    19021 gctaatatgc agaatgtacg tgtttttttt aatcctgaga atttgctcaa tccgggaaag
    19081 atttttccgc atcccggacg ctgtgctgag tccaaagcag gaacacctcc gcctaatctg
    19141 tctgccgtta ataaaacaaa aatggttgcc gcaaaatcag gaatagcagt atgagcaaaa
    19201 tagaaccggg ctccttggaa gaattgcaaa attgggttaa ctcccgtatt gaaacgcaag
    19261 taccattcag gatctgcgga aatggttccc gatttgtgag cacaatttca ggcaaccaga
    19321 aagatattcc cggcgacatc ctttcactga aaaaactcga cggaacacgc ttttttgatc
    19381 cggatgacat ggtcgttggt gtggaagcag gaatgagtat ccggactcta cagggaatgc
    19441 tggcggagga aaatatggtc ctgcctgtca atccgtggtt tccggattct tgtgttggct
    19501 ctgtagtagc ctgcaatgat tatggaccaa accgcatgga catgggcggg cttcgcgact
    19561 gtatcatcgg tatcgaatat attaacggga aaggtgaatt agtacaggct ggcggaaagg
    19621 tggtaaaaaa cgtttccggt tatgacctga cacgaatgat gttaggaagt caaggcggtt
    19681 tgggtgttat cactgctgtt aattttaagg ttatgccacg gcctgtcgaa ccgcatggga
    19741 tgtttggaat tttccgggat gaagcatggt tgtcccagct caaggagcta cgcaaactca
    19801 ggattccatt gaattggatt caggctttat ctacttcaga ctcaagctgg gttttaggta
    19861 tgggatattc cggaaacaag ctgaatcgaa accgaattga aggtgaaata cgtgaagttt
    19921 ttggtaagac tctgaatatt cagcctgacg gcaaatcata tttcggacag attttcactc
    19981 cgggagaaca aaggtttaca gggtttttac cgtcgattcg tgatgcatgg aaaatggagg
    20041 aatcacattt tcatgttttt gcaactttgc cgactgaaga aatctttacc ttcccttttg
    20101 aaactttccg aaaagcaaat ttcaaaattg cagttcatcc tacaggaggt gatattaatt
    20161 ttatgcacaa aagcacagaa cggaaagagc agcttcagca tcttgaaaaa ataaaatcag
    20221 ccctgattca tgctgacagc aggctccggt gggtgagttc tacaccgaaa tgctctttta
    20281 tggatctggg aaaatttggt gtggccagtg gatattcttt ggtaaagaga cttaaaaaac
    20341 accttgatcc tgcaggtgtt ttttttgctc catattatga tttagagttt gacgaatgag
    20401 cataaatttt tcgaacaaaa cggctgtttc gactaaggag cttttccgcc aggccgaatt
    20461 tgataacatt ctaaaatgtg tacattgcgg actctgcctg gaaagctgtc ctacatatcg
    20521 tgaattggaa gatgaaaaag attctccacg cggtcgactt tatttaatgc gtggactctg
    20581 ggagggagaa ctggaactgg aacaatcagt aattgatcct ttaagccgct gccttgattg
    20641 ccgtgcctgc gaaagtgctt gtccttccgg agtaccttat ggagaactgc tggaaaaaac
    20701 gcgcggaatt attctggaaa acacgcctca aagtttgaaa gagcggtttc ttcggaatct
    20761 cttgttaaaa gggttatttc gctacaccag cataatgact gcagcaagcc gtatcttgaa
    20821 aatatatgct gccacaggat tgcccaaatt tattactaaa acatttattg gaaaactgct
    20881 tcctaaatca tttgtttttc aacaacacct gttgcccaat tgttcaggag aatcgttcaa
    20941 acgcaaatat gccgatcaaa tcctttcacc tgtttttcca gaaaaaacac tacacactac
    21001 acaaaataga aaatctaaga tcagagttgg attgttttcc ggatgtatta tggatgtttc
    21061 agaatcggca attcatgaat caacattgac actgctgcgt catttcggtt gtgaagtggt
    21121 tgtaccgggg aatcagggct gttgcggtgc acttcatgtt cacagcggtg acagaaagac
    21181 tgcccgtgaa tttgctctga aaaatcttgc tgcctttgaa cctcgtcatt ttgatgcaat
    21241 tatcacgaat gcagcaggct gcggagctca gttgaaggaa tatcatcacc tgttttcaga
    21301 tgaaactaat gggtccgaac aggactgggt cggttttgaa aataaagtaa tagatattct
    21361 cgaattcctt tcgcgcttcc cggaactgct ggagagtctt tcctggcgtg aagatcaaga
    21421 catagtgctt tatgatgcac catgtcatct tatgcacgcc caaaaagtag atgaaaatcc
    21481 tcggcaactt ttgaacagtt tgcctggagt gaaactggta ccgctgaaag aatccaactg
    21541 gtgctgcggt tctgcaggaa tctacaatct tgttcaacct gacctctcac gagccgtgct
    21601 gaagcgaaaa accgaatctg ttcgtcaaac tttgacggaa aacccaaaag cgaaaactat
    21661 tatcaccggt aatcccgggt gcctgtacca aattcgtgcc ggaatcagga gtgaaaatat
    21721 tgatcttcgg atcctccacc ctgcagtgta cttagtggaa cgtttgaaaa aacttggaac
    21781 cagggacatt cctcaaattc cgatttgaag ccgaagaagc aagtgctcaa tccactccac
    21841 aatcactcac ctttccagcg gtataattca agatttgttc gcccattctg atcgaattgg
    21901 ataccttctg cttccagcag ttcctgctga agtaattctc cagatcctcc acttctcgta
    21961 cttattcccc cctgaaaatt gatgactctc tgccatggaa tatcggagtc aaaaggaatt
    22021 gcagatgcag cataacccac cattcgtgcc gtacaaccac caactatatc tgctatttgt
    22081 ccgtaagtag ccaccttacc gacaggtatg agccggatta tttcatgaat ttgctgatag
    22141 attggcgact tcacagaata ttaactgacg gatttcgcta aatctctagt ttttgtttct
    22201 gtaacagaat tttctgcaga ggcaactttc aacttatttt gaacaagttg aagatgctcc
    22261 tgtgactttg ctggtaagga agatccggat ttttcagcgt aagaagaggc gtccaaaatg
    22321 ctaaaatctt tttcaaaaaa agtcatccac tcataatctt cagtcaggct tctcagacgc
    22381 aggatctgat tttcataaat agagataata aaatcatctt ccagcatcgg catgggatgg
    22441 gaaactacct ggaattcagt tgtgcaagtt ccaatttcat tccccaaaaa aaggaaatca
    22501 tccggttcgt tcccgaaaag tgccaccttg atttgacctg aatttccaac aataccaaga
    22561 cgtttatagg aactgttgac gctgatatcg cgtaactttg ccatcacatc ttttgtaact
    22621 actgtctcaa ttaaaggacc ttcttcagga ggattccagc gggaagcaaa attaaaggaa
    22681 ctgattgcag gtttcaggtc cagagtctgc tctttgcttg tactgtgatt tttgacatgg
    22741 agcctgtttg atatcacctg aaactctaat tttgccggat ttttgttctt tagtaattta
    22801 aaaaattttg cctctaatgg aatagaccat actggttcat tttccggaaa ttgtgatgtc
    22861 agattagcag aaacgttacc cgattttata aaaacatttt ttgcagttga ttcaaggaca
    22921 agatgtagag cttcatctcg aaatatcagc agcattctac cattaaagtc cttttccaga
    22981 tcaacaataa tcttatgcag ttttgccgac attttaaagg aaccatcatc ctcaattttc
    23041 aagaatggag tcaaagtttc agtgtaagta tctccttttt gctggaaatg cccattcagt
    23101 tttttttcat tccaaaaagt gagtaattct gccggatcta ttctacgcac atcgccgggg
    23161 aaactgtttc gtgcggtttg ccatgataat gcttttttcc ccttgatcaa atctgaaatt
    23221 gttttcccgt tatgatctgt ttgctgagtc cgtttggcat tatttattag gaggatagaa
    23281 gcatctttaa aggggtatat tcctgcatta tcaatacaaa tgaaaggtct gaaatctttg
    23341 gttttttcat tcattgaata gaccttgcct gaaaactggt gtttctttta aaagttatga
    23401 taatattcct gcaaaaaaat caccagaaac atggagcgag aaacgggg
//
LOCUS       DTVD01000175            1704 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_32787_length_1704_cov_190.462098, whole genome
            shotgun sequence.
ACCESSION   DTVD01000175 DTVD01000000
VERSION     DTVD01000175.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32787_length_1704_cov_190.462098"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="EYO68_06120"
     CDS             complement(<1..698)
                     /locus_tag="EYO68_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HIC07417.1"
                     /translation="MNSKNEISENNVTDTSSDELHEKLEEMVATVDTGVRNLRSWAGW
                     VLTSVSLAWSLFQLWIASPFPYMLADLIPLLNSTHTRSAHLGFAIFLAFVAFPALKRS
                     PRTKVPMQDWIFGLTGTSCALYIAVFSDQLALRPGLPITPDLIISGIGLVFLLEATRR
                     ALGLPMMCVALLFLSYVFFGEYAPDIIAWQGASFQKAMSHMWLTQEGVFGIGLGVSTG
                     FIFLFVLFGALLEKA"
     gene            complement(806..>1704)
                     /locus_tag="EYO68_06125"
     CDS             complement(806..>1704)
                     /locus_tag="EYO68_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIC07418.1"
                     /translation="EQTFVTIGTGGVTGVYYPTGGAICRLVNKKRKEHGIRCSTESTG
                     GSVYNINTIRAGELDMGVAQSDWQYHSYRGTSKFKDQGPFKGLRAIFSVHPEPVTILA
                     RRDSGIRHISDLKGKRVNIGNPGSGTRATWEVIEEALGWSRSDLKLAAELRSAETGQA
                     LCDNKIDAYFWLVGHPSGLTKETVSSCDAVLVEASGSGIAKLVKDNSFYRWATIPGGM
                     YSGNPNDIKTFGVGATFVTSTRVPANVIYHVVKGVFDNFDQFKKLHPAFRHLNEREMI
                     TDSLSAPLHEGALRYYKERGWM"
BASE COUNT          523 a          414 c          369 g          398 t
ORIGIN      
        1 gctttttcca gtaatgcacc aaaaagaaca aacaaaaaaa tgaatccagt ggaaacgccc
       61 aagccaattc caaaaacacc ttcctgtgtc agccacatgt gagacatggc tttctgaaat
      121 gaagccccct gccatgcaat aatatccgga gcatattcac caaaaaacac ataactcaaa
      181 aacaaaagcg ccacgcacat cataggcaaa cccagtgcac gtctggttgc ttctagtaaa
      241 aataccaacc caatcccaga aataatcaaa tccggtgtga ttggtagccc cggacgaagt
      301 gccagttgat ccgaaaaaac tgcaatgtac agagcacagg aggtaccggt caaaccaaat
      361 atccagtctt gcatcgggac ctttgttcgt ggtgagcgct tcaatgcagg aaaggccaca
      421 aaagccaaaa atatcgcaaa tcccaagtga gctgagcgag tatgggtact attcaaaagg
      481 ggtattaaat ctgccagcat gtacggaaaa ggtgaagcga tccaaagctg aaaaagcgac
      541 catgccaggg aaacagaggt taaaacccag ccagcccagg aacgtaaatt acggacacct
      601 gtatcaaccg tagccaccat ttcttcaagt ttttcatgta attcatcgga tgaagtatct
      661 gttacgttat tctcagagat ttcatttttt gaattcatag agaaaacgta tttaataaag
      721 attgatgaaa atcgggaaag aaagggggaa aagcagagcc ggaaagcgaa caacactttc
      781 cggacaatgc ctcaatcagg tatgatcaca tccagccgcg ctctttgtag taacgcaaag
      841 ctccttcatg caatggagca gaaagtgagt ctgtaatcat ttcgcgctca tttagatgac
      901 gaaatgccgg gtgcagtttt ttgaactgat caaaattatc aaacacacct ttgacaacat
      961 gataaatcac gtttgccggt acacgtgtgg aggtaacaaa agttgcacca actccgaaag
     1021 ttttgatatc attcgggttt cctgaataca taccgcccgg aattgtagcc cagcggtaga
     1081 atgaattatc cttgaccaat ttagcaattc cggaaccgga agcttccact aggacagcat
     1141 cacaggaaga cactgtctct tttgtcaatc ctgaaggatg cccgacaagc cagaaatagg
     1201 catcaatttt attgtcgcaa agtgcttgtc cggtttctgc agaacgcagt tccgctgcca
     1261 attttaaatc acttcggctc cagccaagtg cttcctcgat cacttcccag gtagcacgcg
     1321 ttccggaacc gggattcccg atattgacac gcttgccttt gagatcgctg atatgacgaa
     1381 tcccggaatc acggcgagca agaatggtca caggctcagg atgtacggaa aaaatcgcac
     1441 gtaatccttt gaacggacct tgatccttga acttgcttgt tccacgatag gaatgatact
     1501 gccagtcaga ctgggctaca cccatatcga gttctcccgc acggatggtg ttgatattgt
     1561 aaacggaacc tccggtactt tccgtggagc atcggatacc gtgttccttt cgtttcttgt
     1621 taaccaggcg gcaaatcgca ccaccggttg gataataaac acccgtcact ccaccggttc
     1681 cgatggtgac gaacgtttgc tccg
//
LOCUS       DTVD01000176            1702 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_32841_length_1702_cov_391.034608, whole genome
            shotgun sequence.
ACCESSION   DTVD01000176 DTVD01000000
VERSION     DTVD01000176.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32841_length_1702_cov_391.034608"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="EYO68_06130"
     CDS             complement(<1..656)
                     /locus_tag="EYO68_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIC07419.1"
                     /translation="MLKKNDYTSNSLIYGIVLVFFMLTSVTAGAMEQYPYFDPQKGYG
                     IENDARPWFDGKDYPFFREMYNGKSLKPQEEGSYQNFPKKSVPVRVVLGKVVRIYDPF
                     IPAVHGDGAGTKGNPREFSPKNPTQASSDSIKRGQTLYNTYCAACHGEDGKSNTVAVE
                     KGVPAPPITAFFQIPTAAAHLYNKIKYGSFFQEPRGFMPSYGAQTSVRDRWDMVNYMM
                     "
     gene            complement(640..1161)
                     /locus_tag="EYO68_06135"
     CDS             complement(640..1161)
                     /locus_tag="EYO68_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIC07420.1"
                     /translation="MSKFTGVWAIFEYLDETTETIEQVREQGVQPTVLMPCPRHEIDH
                     ALGEPGTLVPWIALAFGALGCLIGFSLPAWTASDWVLPVSGKPIVAIPPFTIIGFELT
                     ILLTAIFTLLGLFLLGFIDSCRFPIPKAAKKYRRFQRDRFGVVVRCDSSRIGEFESIM
                     KKNGAEEVHVEKE"
     gene            complement(1154..>1702)
                     /locus_tag="EYO68_06140"
     CDS             complement(1154..>1702)
                     /locus_tag="EYO68_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HIC07421.1"
                     /translation="NHVIQSVSKMIIFTSIIVGYAYAAEFFIGYYSGSPFERAIFYYR
                     PFGEMQSWTLLGENQINFPQIAFWLMVFCNVIAPIPLWRWKIRNNPLAVWIITILINI
                     GMWFERFNIIGSSLQHEYDPASWGEYWPTIVEVGVTIGSFGFFFTLFTLFAKSLPPMA
                     IMELKEGTNPPMKHAAKKDINE"
BASE COUNT          510 a          391 c          335 g          466 t
ORIGIN      
        1 ctcatcatat agttgaccat gtcccaacgg tccctcaccg aagtctgagc tccgtatgag
       61 ggcataaagc cacgtggttc ctggaaaaaa cttccgtatt taattttgtt atagaggtgc
      121 gcagcagcag tgggtatttg aaaaaacgct gtgatcggtg gcgccggtac acctttttca
      181 accgccactg tatttgattt tccgtcctca ccatgacaag cagcacagta agtgttatat
      241 agagtttgac cgcgtttaat cgaatcggaa gatgcctgcg taggattttt tggagaaaat
      301 tcccggggat tacccttagt tccagcacca tctccgtgaa ctgccggaat aaatggatca
      361 taaattctga caactttacc caagacaact ctcacaggaa cggatttttt tggaaaattt
      421 tgatagcttc cctcttcctg cggcttcagg cttttcccat tatacatttc acgaaagaag
      481 gggtaatctt ttccgtcaaa ccaggggcgt gcatcatttt cgattccata gcctttttgc
      541 gggtcaaaat acggatactg ttccattgct cctgccgtta cagacgtcaa cataaagaag
      601 actaaaacta ttccgtaaat aagactattt gaggtgtaat cattcttttt caacatgcac
      661 ctcctctgca ccgttctttt tcataatcga ttcaaactca cctattctag atgaatcgca
      721 gcggactacc acaccaaaac gatctcgctg aaaacgccta tattttttgg ccgccttcgg
      781 aatcgggaag cgacaagaat caatgaatcc caaaagaaac agtcctaaca atgtaaatat
      841 agccgttaac aaaattgtca gctcaaaacc aataattgtg aaaggtggaa tggcgactat
      901 aggtttaccg cttacaggta aaacccaatc tgaagcagtc caggctggta agctgaaacc
      961 aattaaacaa cccagtgctc caaaagccag tgcaatccag ggaaccaatg ttccaggttc
     1021 acccagcgca tggtcaattt catgacgcgg acaaggcatc aatacagtgg gttgaactcc
     1081 ctgttcccgg acttgttcaa ttgtttcagt ggtttcatcc aaatattcaa aaatagccca
     1141 cactcctgtg aatttactca ttaatgtcct tttttgctgc atgtttcatg ggaggatttg
     1201 tgccttcctt taactccatg attgccattg gaggtaaact cttggcaaac agagtaaata
     1261 gagtaaaaaa gaatccaaaa cttcctatag tgacacctac ttcaacaatc gttggccagt
     1321 actcacccca ggaagcagga tcgtattcgt gttgaagtga actgccgata atattaaaac
     1381 gttcaaacca cattcctata ttgattaaaa tggtgatgat ccaaacggcc agagggttgt
     1441 tcctgatttt ccagcgccac aaaggaatgg gagcaatcac gttacagaaa accatcagcc
     1501 agaatgctat ttgggggaag ttgatttggt tttctcccag taatgtccag ctttgcatct
     1561 caccaaacgg acgatagtag aatattgccc gttcaaaagg actgccgctg taataaccaa
     1621 tgaaaaattc tgcagcataa gcgtaaccga caattattga agtaaaaata atcatcttcg
     1681 aaacactttg gatcacatgg tt
//
LOCUS       DTVD01000177           23237 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_328_length_23237_cov_477.834786, whole genome
            shotgun sequence.
ACCESSION   DTVD01000177 DTVD01000000
VERSION     DTVD01000177.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_23237_cov_477.834786"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            112..1014
                     /locus_tag="EYO68_06145"
     CDS             112..1014
                     /locus_tag="EYO68_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07422.1"
                     /translation="MNNMNNRLTQPTPEVPEELEIQTSTLRLVAKCWGKPEGLPVLAL
                     HGWLDNAATFDHLAPFLPEFRLVSLDLPGHGFSDHRPSGTSYHFTDMVVDVLEVAEVL
                     KWDHFSLLGHSMGAGIASYLAGTIPEKIKYLMLIEGLGSIVQAPEKMPENLREATNQW
                     LRRSDKKLPLYPNMETAIKVRHNTGSIAVSSVRTLVARGLRIVDGGFTWRSDPSLKIR
                     SRHYLIKEQACAFLQKISAPVLLIEAKNEKKDQWNELMQELIPHVKNLQHRIITGDHH
                     LHLDNPESVADVIHEFLNDFSENS"
     gene            1011..1826
                     /locus_tag="EYO68_06150"
     CDS             1011..1826
                     /locus_tag="EYO68_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIC07423.1"
                     /translation="MSLESKLESGNQNQDNDNRLQVSSKGKRLFALLLDFIFALLFAN
                     TLVQVFREEHWDLVMQSRDLSGLVFFYGSIAFILLFKDIFGRSLGKLLLAMTIREIEN
                     LEQRPSRTVLVQRNILLLLFPVEGVIVLRDAYARRLADKWWKTVVLDDQKAMRGTLRI
                     LLGNIILFGFFSIAILFQRSGIEKTAAYQTAEQAIRSHQPLISLLKQSPEIEEPEMHL
                     DLRENAENPSLVRARIGDEETGKEVTVSLTFRKNPPGWEVLNIEVKPISEAED"
     gene            complement(1843..2244)
                     /locus_tag="EYO68_06155"
     CDS             complement(1843..2244)
                     /locus_tag="EYO68_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HIC07424.1"
                     /translation="MSIPRRGHETDAGIDLTAMAVEQKRPDIFFFDCGISIHVSNGFY
                     VEIVPRSSIIKTDFILANSVGIIDPDYRGRIFIPFRYVGSGDGMQAAEELLHQRIAQM
                     LVCKLEPCRIEAVDSLEDSVRGEGGFGSTGQ"
     assembly_gap    2347..2446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2549..3850
                     /locus_tag="EYO68_06160"
     CDS             2549..3850
                     /locus_tag="EYO68_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07425.1"
                     /translation="MPLDTKTICRLLQNANRPELAKILGEVWETPLLDYADQLWEKPV
                     APPPFEIELREAFETEFCRIGYTEIEAKAYSTALDRTRVLQTATHLTVSEGPTFLALH
                     HLSLLGLPVAETYFVGAFSGVPFANAAWSGCLNFSNRFDLETVIDPKAPGFAELKRAE
                     SDRYRDSTERRISFIPGSMRDSRVYQSKVPEKLTRLLPYIAEPIRKYVPVVKPGDEFT
                     AWASQFSAAQLRKIMPGKSVLYFDLNEVIRTYLILVLKNSQHPLFRFLFEPTIRKTVL
                     DEFSPETPLFTVEVHHKNKIRQETVVFKDDMLQSQNFQLEVSPEKIIKALESGTLCPG
                     LFITFTTLCFINALICFGSFEQVEYLAEFRRKWLKLGFLEQEIVRAVNTSALTSGRCI
                     EESGVAVNPLDLLLGFRWSFMENQTVGELMRPLLPRLGIEV"
     gene            4051..5616
                     /locus_tag="EYO68_06165"
     CDS             4051..5616
                     /locus_tag="EYO68_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIC07426.1"
                     /translation="MDISVLPILSLIIFSPIVGIALAAIFRDEGNGIPAKVSALFSSG
                     ISFLLSLHLWFNFDSSTADLQMVERIPWITQFNIEYFLGLDGLNLFFFLIITFITPLA
                     LLGSWNVAKRNWQFQIQMLLLQIGMLGCFAAMDVVLFYVFFEVMLVPMYLLIGIWGGP
                     NRLYATIKFFIYTMLGSLLMLISLLYTANIYLDLNGAWSFSVLDWYGMQIPPETQFWL
                     FLGFAAAFAVKIPLFPLHTWLPDAHYEAPTAGSVQLAAVMLKFGPYGFLRFAMPIFPA
                     AVFDLTPIFMVLALIGIIYGGLVAMVQPQIKRLIAYSSVAHMGYIVLGLFALNIQGLS
                     GSWIQMINHAIVTGALFLTVGMIYERTHTQEISAYGGVAHTMPKFAVFLVFFSMASIG
                     LPGLNGFVGEVLTMIGAARVNIWYGIVAAFGVLVAAIYMLWMVQRVIFGPLTKDLVKN
                     LNDFSLREVVVLVPLVFWTIFLGVYPQPFFERIEVSINHYIEMIKNQEPRFAQKEAES
                     SGLAKFLVWNFSE"
     gene            5630..7072
                     /locus_tag="EYO68_06170"
     CDS             5630..7072
                     /locus_tag="EYO68_06170"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIC07427.1"
                     /translation="MSLQIYNSLSDQKEPFNPVHEGQASLYVCGPTVYSDSHLGHAKA
                     YVSFDVILRYLRFCGLKTLYVQNITDVGHLMGDADEGEDKLLKRARELNQEPMAVAES
                     YSRRHFEAMDLLGVIRPDISPRATGHIPEQLEAIEKLLEHGLAYESNGSVYFEITKDP
                     KYGELSNRKVEEMKSGARVKVRSEKRHPGDFALWKRAEPGHLMRWRDPFSGWGYPGWH
                     TECAVMSTRYLGEEFDIHGGGMDLKFPHHECEIAQARGLEKPFARNWMHTNMLTIEGQ
                     KMSKSSGNFVTLFDVFKKYDPLTVRYFIAASHYRSVVDFSESALTSAGLSMKRLHQTV
                     RNLRERKADGAKPAGKYFEEFRKRFCKAMDDDFSTPQAIAVLFDLSRDVNTLLSEKTA
                     EPEAIVDAEHLFSSLGGDVLGIIPESLEASQTELQIKNVMEVLVELRENFRENKDFAS
                     ADLIRERLQKIGIEFKDLPEGTTWEVTDNK"
     gene            7117..8913
                     /locus_tag="EYO68_06175"
     CDS             7117..8913
                     /locus_tag="EYO68_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07428.1"
                     /translation="MMDNQSRHIAWRLYELIAPLKGWVFFSIVCMVGYNIFTAAPAYY
                     AKDIVDAIAYGDNPELKQYFLVGLGMIIVFAIKGLFFFGHNYSVGHLVQSLIVKLRQQ
                     LFDHLVNLSLTFFNRSKTGDLISRFTNDLHVFQNMLQVGVTGPFRDIPQFFLLLAIMF
                     FRSWQLALVTMTIIPVAIFFIQIFGQRNKVAVSQRQISFGDLSSLLVETITGVRVVKA
                     FGMEKYESKRFKDANDDLYKNHMRSIMIDSYSYPVIEIIGAAAGATIVAYGGYLIIND
                     QITAGDFTSFVISFFLLNEPVKKLNGFNLKLQEGIAAVQRIFNILDVKDDIVISPDAT
                     KLTSFDREINLNIQAFHYPDQDEPAVKDFQLTLQKGEAVALVGSSGAGKTTIANLIPR
                     FYDITRGEVYIDGHDLRDLDIASLRKLIAIVTQDTILFNDTIASNITYGQPDCSKERM
                     YAAAQAANAHKFILEQSDGYETVIGEKGARLSGGQRQRLSIARALVKDAPILILDEAT
                     SALDSESEIEVQQAIEHLMENRTTIVIAHRLSTIRNADRICVMENGQIVEQGTHNELL
                     SKEGRYQQLYEMQFQDQPNGSVSEESKSRLVS"
     gene            8920..9741
                     /locus_tag="EYO68_06180"
     CDS             8920..9741
                     /locus_tag="EYO68_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIC07429.1"
                     /translation="MNKSPFSRINTAVILAAGMGVRLRNVIGVCPKGLLEIEGKSLII
                     RSLERLKSEGIDRVIIVTGFQDTMYHKHLEPLANLPKIEFIHSPRFAETGSMHSLFVA
                     QEVLQDDFLLLESDLLYESRALSSVLNYEGPDVVLASGKTGSNDEVYIYGINQGKNHN
                     YETSSSIVSGDIAAISKQPRPHLQIQGELVGISIISQNLFRRMCALHEENLTFPCSNH
                     YEECISEVSSEWVVPYLRVGDLVWTEIDDQFHFDRAIKIIYPRIKQQETSNKTGL"
     gene            9738..10115
                     /locus_tag="EYO68_06185"
     CDS             9738..10115
                     /locus_tag="EYO68_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC07430.1"
                     /translation="MSDSYVLQQAPFVVPTTDGKLIEEHVGRAVSNEPEVSVAHMVAP
                     PGWSEPHQNPEFDEYTMMVRGRKQIEIDGETVTLNVGESLLVRKGARVRYSNPFDVEA
                     EYWSVCMPAFSPDLVNREEDSGS"
     gene            complement(10104..11345)
                     /locus_tag="EYO68_06190"
     CDS             complement(10104..11345)
                     /locus_tag="EYO68_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07431.1"
                     /translation="MFRLRFQGVRLQLHWNKIIQKILEFLIRRLLLKGDSLFKAQPYL
                     RQEKQTLLDKSSGRRIIYFTFRTLHFLDWFAPIDLALERSFPGKYEVFYIDFSTTLHR
                     IGKGFEFLHFRQQVEERLLQLNISPLRHFSHEELSEYTSFPDADVHVTCESIRQETFS
                     VPERIYLPHYALPKAIDIGLPENIRFNHVFLPTRPPYTYKQLEQKFQEEIKVHSVGYP
                     KLQAASSPVSCFPDSENPVVLYAPSLELSLLFDALDRGLLEIFNKMSHYNFVIKLHPS
                     LASRRHYVTAFMCQELKGVEHIHFDELSGIQNLAEESSLLITDFGSVGGEYRLGYGKR
                     VIFLKTPAEYEGGADLQFRDDFADGVCKVEDLGNVIESVMKKGALSDSEFHNMRQQVL
                     SFSEASDYEAARIVDEICSAS"
     gene            complement(11381..12457)
                     /gene="leuB"
                     /locus_tag="EYO68_06195"
     CDS             complement(11381..12457)
                     /gene="leuB"
                     /locus_tag="EYO68_06195"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIC07432.1"
                     /translation="MITKKILVTPGDGIGPEVTKQAIHVLKTVAPRFELQLELSEKPV
                     GGVAYDLTGTPIPDETLEAAKNSDAVLLGAVGGPKWEPLDFSVRPERGLLKLRSELEL
                     YANLRPAVIYGDLVNASTLKPEIVDGADLMVVRELTGGIYFGEPRGVEIRNGERVGFN
                     TLVYSESEIRRIVKVGFEIAMKRKKKLTSVDKANVLESTELWREIATEVGKDFPEVEL
                     SHMYADNAAMQIIRNPKQFDTMVTTNMFGDILSDAAAMMTGSLGMLPSASIGGKNGMY
                     EPVHGSAPDISGQDKANPLAMILSVGMMLRYSFDLTEADEMIQQAVVKTLETYRTDDI
                     MAEGKTLIGCQEMGERVLQILEQK"
     gene            12525..13757
                     /locus_tag="EYO68_06200"
     CDS             12525..13757
                     /locus_tag="EYO68_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 4"
                     /protein_id="HIC07433.1"
                     /translation="MQEKMFRLMLGTGIIILLSSVLWAANPSQSKLDIQLKSLVRHGS
                     VLIANEQQVLYRYPPNKNPLLIPASVLKYATALSALHYFGSEYSFNTEFYIDQNHSLV
                     IKGKGDPFLVSEEWQRIAQQISLLPDVPKKMQGLFFDTSLFSEKIKIPGISFSNNPFD
                     AHNGALVVNFNTVFVKVDSKGHVTSAEKQTPLTPLARRLAKKLSPGTHRISIQPNLSF
                     TYSGELIKSFFRKEGFSFENKNVALRSVRNGDHLFYTHSNILTLKEVIAGMLRFSNNF
                     TANQLLLTVGLQRYGPPATLEKGTRALTEYLRQEVKLPKEQFKLVEGSGISRKNRLTP
                     EAIWQLLKDFAPYQDLLHEDNGVLLKTGTLRGVYTMAGYLPGTQPLYFAILLNQSQNY
                     RNKILQILLSTDFSAGKF"
     gene            13754..14821
                     /locus_tag="EYO68_06205"
     CDS             13754..14821
                     /locus_tag="EYO68_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC07434.1"
                     /translation="MKSNPIISILMPVRNDAAMLRNCLTDIQNQSFTNYELVVVDDGS
                     ADETPVLLEKASQDDSRIRMIRTEPQGIVSALNTGLIECKGTYIARMDVDDRMQKTRL
                     EKQLELMQKDPELELIGCRVEGFTDKGPLPESAVHYQSWSNSLMSHQQIECDLFAESP
                     IVHPTFFATRKLFIKIGGYSNNPWAEDYDFILRAYGGGAKLAKHPEILVRKYFAPGRL
                     SRVEAMYKRPAMFDAKAHYLLEFGLLKKRRGVLIVGSGPTGRQAAQSFENRGVRLLGF
                     VDNRPGPPDRQVKNRPAWGFNNLPPEEFMHKFRDSLIVLAIGDSTGQRVFAEFLRKSD
                     FVENHDFVRVIYNWPPVANSI"
     gene            14937..15503
                     /locus_tag="EYO68_06210"
     CDS             14937..15503
                     /locus_tag="EYO68_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIC07435.1"
                     /translation="MTLSKINLLRLFFLLWLLVFVTPSAQSKQAPLAGEERKLLNSLR
                     ITPATAWIEAHNFAGKLPDEKTVNLKDYRGRFILLNFWATWCSPCLKEMPDFEKAYQQ
                     MGQEKLIVLAVGMGEDTKKISKFAEKYGFTFPMVADPKLEITNLYGVKNIPVTYLIDP
                     EGVVLGRALGIRDWANPDLLAFIKSQLK"
     gene            15651..16058
                     /locus_tag="EYO68_06215"
     CDS             15651..16058
                     /locus_tag="EYO68_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HIC07436.1"
                     /translation="MAGTNPYIEKAEFELPLQNYDVTFLPMQKKVAVLSEKITTESDG
                     LPGSILNAALEAGIDMDHSCGGVCACSTCHVIVRQGLESCNEATDDEEDMLDLAPGLE
                     PQSRLACQCVPNGTQALIIEIPEWNRNLVSEDH"
     gene            16072..16440
                     /locus_tag="EYO68_06220"
     CDS             16072..16440
                     /locus_tag="EYO68_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HIC07437.1"
                     /translation="MITLTENAAKEIQKIMLENELAENVCVRVGVKGGGCSGFTYTLD
                     FDSKKTKFDLEFESQNLTILVDKKSHLYIKDTEIDWSYSLMDRGLKFNNPSAKGSCGC
                     KTSFMFEPPEQEEDFKPSWM"
     gene            16460..16714
                     /locus_tag="EYO68_06225"
     CDS             16460..16714
                     /locus_tag="EYO68_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIC07438.1"
                     /translation="MSLAGGQKTIEEQIKEAIEKSVADSTAEVGGDGRHFEIQVISPV
                     FEGKRTLEKQRIVYAALNDLMSGSNAPVHAIARLETLVPE"
     gene            16753..17052
                     /locus_tag="EYO68_06230"
     CDS             16753..17052
                     /locus_tag="EYO68_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07439.1"
                     /translation="MKIEEKRVTFPKTLSVKYAGKIVEETATHLTLEGEDEDSYLKIF
                     TPFRGVAKLLLFENDKWVDAENSSAVSNFDFSSLGLGDLSTLSAGDEADSSGTKK"
     gene            17243..17731
                     /locus_tag="EYO68_06235"
     CDS             17243..17731
                     /locus_tag="EYO68_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIC07440.1"
                     /translation="MSETKISDGLPQIQIQTDRGNMTIEMFEDDAPNTVANMISLIEK
                     GYYDGLNFHRVIPDFMIQGGCPQGTGTGGPGYDFDDECTPERRHDSAGILSMANAGPG
                     TNGSQFFITHGPTPHLDGKHTVFGKVTEGLEVVNEIKQDDVMEKVLVLQKRDHVYEVE
                     TL"
     gene            17772..17989
                     /locus_tag="EYO68_06240"
                     /pseudo
     CDS             17772..17989
                     /locus_tag="EYO68_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            18023..20593
                     /gene="gyrA"
                     /locus_tag="EYO68_06245"
     CDS             18023..20593
                     /gene="gyrA"
                     /locus_tag="EYO68_06245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIC07441.1"
                     /translation="MSETILEADIVHEMRDSYMEYAMSVIVGRAIPDVRDGLKPVHRR
                     VLFSMSEQGNDFNKPYRKSARVVGDVIGKYHPHGDTAVYDTIVRLTQDFSMRYQLIDG
                     QGNFGSVDGDSPAAMRYTEVRMRKIAHDMLADLEKDTVDFIPNYDDSLEEPTVLPSRI
                     PILLTNGSTGIAVGMASNIPPHNLKEVLNALLFYIDHRNSATVQELMEFIPGPDFPTG
                     GTIIGKSGIYDAYTTGRGIIRIRAKSHFEQTKDGRDMIIVDELPFMVNKASLLEKIAE
                     LVRHKKIEGISDLRDESDRKGMRMFIQLKRGETPDVVLSNLYKHTALQSSFGIIMLSI
                     VDKQPKILALTQMLEFFLQHRIEVVTRRTRYELKQAENRMHLLEGLMIALENLDAVLN
                     IIRKAESGGVAEDVLMESYGLSKRQAHGILDMKLQRLTGMEQDKIRTEHEDVGKAIED
                     YKDILEKDERVIEIIRKESIEIRDKYGDERRTEIIEGDSSILIEELINEEDMVVTLSR
                     EGYIKRSSVSEYRLQRRGGKGRLGMGTKDEDFVEQMFVASTHDFLLFFTDKGQVFRKK
                     VFELPLAGPTGRGKAAINLLPLREGESICSCLNVPKEAENKYIFLCTEGGVAKKTPML
                     DCAKIRVTGLRVITLDEGDSVISVQLTDGNQELFFATRDGMGLRVNESTLRAMGRQAR
                     GVRGVKLRKGDRVVSALALSGTGELLTVTEGGYGKKTPIVDYTLAKNRGNFGMRTIRI
                     TDTNGPVVNVLEVEEHFQLFLITQLGKLIRIPVENIRTIGRVTQGNRLIRLETDEQVI
                     GIAPVIEDEDDDEVEVKEENSTDSTTVSAAETAPENLVEENIPEEPQDPSDSDEPSDS
                     "
     gene            20697..21605
                     /locus_tag="EYO68_06250"
     CDS             20697..21605
                     /locus_tag="EYO68_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HIC07442.1"
                     /translation="MKQFFLLLVLSLLFLFPTAGLSLSVSSGHMLDRFELIPDEKTPF
                     LTFKGFFDPGQFPHIQIFPRNSKTETSVLLPNTLINSILLPEREVTSFSEEGIMEKML
                     LEEKITKKENGEIEFQVTLTISSTEEHILVLDTEKSDSRHLTFALQKHVKKETTNAQE
                     QQKVESIIIDLAPPAISPREEMLLHPVTALMSYRRVDQLNLTILNASPHPDSAQRLAV
                     LLDRQQKRTIEKRIGMKLKIVNISSVREQTILPKTKIYFHANLMREALILAQILPGEQ
                     VIVPMPQSRISKLATDVEIYVGKNFE"
     gene            21583..23100
                     /locus_tag="EYO68_06255"
     CDS             21583..23100
                     /locus_tag="EYO68_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07443.1"
                     /translation="MWERILNKKIYFISLALALFVTGFSLPAFAFPEYTSRQKALKLA
                     GVLDHGYFKSIKISSVFVKNRGKDDYYLQAILDDGSSRKWLINRIREWAQTDELILSK
                     NRALVFPTPESTDFGVLDKNKFYRIILNSTAFVKTFGKHDILDGKSLVLGIRRFRILQ
                     AEDSKYMKTDKFGDRYRYVLELENGSREFFTYSDAFLFLQRTALLEKAPENVSVLRKT
                     FKVRELKKIPLQIEDELREIRRFGIEVVFDGPILMSPDRFPFQVVEQKMRDPETGRYK
                     AQFFLQIIFPNSEKIREIPGFNNHEYLRYVEIDTDVEHQKRVILSAMVNPNALDLPPY
                     IEVTDRNSVVVNFFSSTDQSLIQPPDLLASHSAGELPRSVFTPDQLETEFETHYMEAV
                     GLIRTAQNEMNMSVKIETYFKALDTLKKAALTAEFDIQIAQALNQRDILLLTMPKLII
                     RNVQMTILALQLDDLNIKPDPVLTVKLLKQLNHAENYASTQDQRQKIASLQSILR"
     gene            23161..23237
                     /locus_tag="EYO68_06260"
     tRNA            23161..23237
                     /locus_tag="EYO68_06260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:23195..23197,aa:Pro,seq:cgg)
BASE COUNT         6738 a         4618 c         5123 g         6658 t
ORIGIN      
        1 tcctgagagc agtgtcaaga tgctaaaaaa ttgtggacat tggctgcatg ttgaacagcc
       61 agaatctttt caaaaaacag tttccgcatt tttgcagcaa aatgggttat tatgaataat
      121 atgaacaacc gcttaactca accgacacca gaagttcctg aagaattaga aatccaaact
      181 tctaccttac gtttagttgc aaaatgctgg ggtaaacctg agggtctgcc ggtacttgct
      241 ttacacggat ggctggataa tgctgcaacc tttgatcatt tggcgccatt cctaccggag
      301 tttcgactag tctcacttga cttgccggga catggatttt cagatcatcg tccatctgga
      361 acgtcatatc atttcacaga tatggtcgtt gatgtacttg aagtagcaga agttttaaaa
      421 tgggatcatt tttccttgct gggacattca atgggtgcag ggatagcctc ttatttggca
      481 ggtacaattc cagaaaaaat taaatatctt atgttaattg aaggcttggg ttcaatagtt
      541 caagcaccag agaaaatgcc ggaaaattta cgggaagcaa ctaatcagtg gctacgccgt
      601 tcagataaaa aattgcctct ttatcctaat atggaaactg ctataaaagt acgccataac
      661 actggaagta ttgctgtgtc gtctgtaaga actttggtgg cacggggact gcggattgtg
      721 gacggcggtt tcacctggcg cagtgatcca agtttgaaaa ttaggtcacg ccattatttg
      781 ataaaagaac aagcctgtgc ctttctacag aaaatttctg caccggtact gctgattgaa
      841 gcaaaaaatg agaagaaaga tcaatggaat gaattgatgc aggaacttat tcctcacgta
      901 aaaaacttac agcaccgtat cattacggga gatcaccatt tacatctaga taatcctgaa
      961 tccgtagctg atgtgattca tgaattttta aatgattttt cagaaaactc atgagtttag
     1021 aatctaaact ggaatccgga aatcaaaatc aggataatga caaccgcctg caagtttcat
     1081 ccaaaggcaa gcgtttgttt gccttgttgc tggattttat ttttgccctg cttttcgcaa
     1141 atacattagt gcaggtattt cgtgaagaac attgggattt agttatgcag tctcgagatt
     1201 tgtcaggttt agtgtttttt tatggaagca ttgccttcat tttactgttt aaagatattt
     1261 ttggcaggag ccttggtaag cttctgcttg caatgacaat tagggaaata gagaatctgg
     1321 aacagcgtcc ctcaagaact gtgcttgtgc aaagaaacat attgctgctt ctctttcctg
     1381 ttgaaggcgt gatagtcctg cgagatgctt atgccaggcg gttggcagat aaatggtgga
     1441 aaacagttgt acttgatgac caaaaagcca tgcgtggaac tttacgtata ttacttggca
     1501 acatcatttt gtttggcttt ttttcgattg caatcttatt ccagcgttcc ggtatagaaa
     1561 aaactgctgc ataccagact gccgaacagg cgatacgttc acatcaacca ctgatttcac
     1621 tattgaagca atcaccagaa attgaggaac cggaaatgca tctcgattta cgggaaaatg
     1681 ctgaaaatcc ttccctggta cgagcacgta ttggagacga ggagacagga aaagaggtta
     1741 ctgtttcttt aactttccgg aaaaacccgc cgggctggga agtactgaat attgaagtaa
     1801 aaccgatcag cgaagctgaa gattgagaga gccgattatt gcttactgtc ctgtgcttcc
     1861 aaagccgcct tctccgcgaa cagaatcttc aagggagtcg actgcttcaa tacggcaagg
     1921 ttcaagtttg caaaccagca tttgtgcaat gcgctgatga agaagttctt ctgcagcttg
     1981 cataccgtca cctgaaccca catagcggaa gggaataaag attcgtccac ggtagtcagg
     2041 atcaatgatt ccgacgctat ttgccaatat gaaatctgtc ttaattatgc tggaacgcgg
     2101 cactatttcg acataaaatc cattacttac atgaatagag attccgcaat caaaaaagaa
     2161 aatgtccgga cgtttttgtt caactgccat tgcagtcagg tcaatgcccg catctgtttc
     2221 atgtccgcgc ctggggattg acattccgtc aaaatggcag caaattttta acagtggtat
     2281 agagtccaag atactataaa agctttgaaa ctttgaaact ttgaaacttt gaaactttga
     2341 aactttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntact ataaaagctt
     2461 tgaaactttg aaactttgaa actttgaaac tttgaaactt tacagagaaa gtctatgtat
     2521 tcaaataata attttcaagt tataacaaat gccacttgac acaaaaacca tttgtcgttt
     2581 actccaaaac gcaaatcgcc cggagcttgc aaaaatattg ggtgaggtgt gggagacacc
     2641 tctacttgat tatgctgacc aactctggga aaaaccggtt gctcctccac cttttgaaat
     2701 agaattgcgg gaagcttttg aaacagagtt ttgcagaata ggctatactg aaatagaagc
     2761 caaagcctat tctacagcac tggatagaac acgtgttttg cagacggcaa ctcacttgac
     2821 tgtatctgag gggccaactt ttctggcctt gcatcattta agtttattag gtttgcctgt
     2881 tgcagaaaca tactttgttg gagccttttc cggggtcccg tttgcaaatg cagcctggtc
     2941 cggatgcttg aattttagta atcggtttga cttggaaaca gtcattgacc caaaagctcc
     3001 aggatttgct gaattaaaac gtgcagaatc agaccgttat cgtgattcaa ctgaacgacg
     3061 tatctcattc attcccggaa gtatgagaga ttcccgtgtt taccaaagca aagttccgga
     3121 aaaactgacc cgtcttcttc cgtatattgc tgaaccaatt cgcaaatatg tacccgttgt
     3181 aaaaccgggg gatgagttca cggcctgggc ttcacagttt agtgcagctc agctgcgaaa
     3241 aatcatgccc ggcaaatcag tgctctattt tgatcttaac gaggttattc gtacgtattt
     3301 gattttggtt cttaaaaatt cgcaacaccc tctgtttcgt tttttatttg aaccaacaat
     3361 ccgtaaaaca gtgcttgatg aattttctcc agagactcct cttttcaccg ttgaagttca
     3421 tcacaaaaat aaaatacggc aggaaaccgt tgtgttcaaa gatgatatgc tgcagagtca
     3481 gaattttcag ctcgaagttt caccagaaaa aatcataaag gcgttggagt ccggcacact
     3541 ctgtccagga ttatttataa cattcacgac attatgtttt atcaatgctt tgatttgttt
     3601 cggcagcttt gaacaagttg aatatctagc agaatttcgg cggaagtggt taaaactggg
     3661 ttttctggag caggaaatag ttcgagctgt caataccagt gcccttacca gcggacgctg
     3721 tattgaggag tccggtgtag cagttaatcc attggatttg ttattaggtt tcagatggag
     3781 tttcatggaa aatcaaactg ttggtgagtt aatgagacct ttgttgcccc gtttgggaat
     3841 tgaagtatga tgcttagatg cgcaaaacaa tgtttgttca actcagattg atcttgcttt
     3901 aagtggctaa ttctcttagt tttatatata gattttttct caaaactatt atgcgtatac
     3961 aatttcatga agtagaaagt atttgcgaag ttttacaatc acgctagctt aatttaaaac
     4021 cttcacctca aacatttcca accaattcag atggacattt cagtacttcc aatactttct
     4081 cttataatct tttcaccaat agtgggcata gccttagcgg ccattttccg tgatgaggga
     4141 aacggtattc ctgccaaagt aagtgcgctt ttcagcagcg gcatttcttt tttgctgagt
     4201 ctgcacctct ggtttaattt tgattccagt actgcagact tgcaaatggt agagcgcata
     4261 ccatggatta ctcaattcaa cattgaatat tttttaggtt tggatggact taatcttttc
     4321 ttttttctga ttataacctt catcactcca cttgcactgc tcggttcatg gaatgtcgcg
     4381 aagcgcaact ggcagttcca aattcaaatg ctgctcctgc aaatcggaat gctcggttgt
     4441 tttgcggcaa tggatgtagt gttgttttat gtattttttg aggtgatgct ggtaccaatg
     4501 tatttactaa tcggaatttg gggtggtcca aaccgactct atgccaccat caagtttttc
     4561 atttacacaa tgctgggttc gcttttgatg ctgatttctt tgctctatac agcaaacatt
     4621 tatctggatt tgaacggagc atggtccttc agtgttcttg actggtacgg catgcagatt
     4681 ccacctgaaa cacaattctg gctctttctt ggatttgcag cagcatttgc tgtaaagata
     4741 ccactctttc ctttacacac atggcttccg gatgcacact atgaagcacc gactgcgggt
     4801 tctgtgcagt tggcagcagt aatgttgaaa tttggacctt atggtttttt acgttttgcg
     4861 atgccaattt ttccagcagc agtttttgat ttaacaccca ttttcatggt acttgccctg
     4921 atcggaatca tttacggggg acttgtggct atggtacaac cgcaaataaa gcgtcttatt
     4981 gcctactctt cagttgcaca tatgggttat attgtgctgg gattatttgc gctcaatatc
     5041 cagggtctct ccggaagctg gattcaaatg ataaaccatg cgattgttac cggtgcactg
     5101 ttcctcactg ttggaatgat ttacgaaagg acgcatactc aggaaatttc tgcatatggc
     5161 ggggtcgctc acaccatgcc gaaatttgct gttttcctgg ttttcttttc catggcttct
     5221 attggcttgc ctggactgaa tggtttcgtt ggagaagttt tgaccatgat aggtgctgcc
     5281 agggtaaata tctggtatgg aattgttgca gctttcggtg tcctagtagc agctatttac
     5341 atgctttgga tggtacagcg tgtgattttt ggaccactga ccaaagacct cgttaaaaat
     5401 cttaatgatt tttcactgcg ggaagttgta gtcctagttc ctcttgtttt ctggactatt
     5461 ttcctgggag tgtatccgca gccattcttt gaacgtatcg aagtcagtat caaccactac
     5521 attgaaatga tcaaaaatca ggaacctcgt tttgcacaaa aagaagcaga aagttcaggt
     5581 ttagcaaaat tccttgtctg gaatttttct gagtgataaa ttagcaaaaa tgtctcttca
     5641 aatttataat tctctctctg atcaaaaaga accttttaat ccggttcatg agggccaggc
     5701 gtcactttat gtttgtggcc ccaccgttta cagcgacagc catctcgggc acgcaaaagc
     5761 ctatgtcagt tttgacgtta ttttacgata ccttcgtttc tgcgggttaa aaacattgta
     5821 tgtgcaaaac ataactgatg ttggacacct gatgggagat gctgatgaag gagaagataa
     5881 actgctgaag cgtgccagag aactgaatca ggaaccaatg gcagttgcgg aaagttattc
     5941 tcgcagacat tttgaggcaa tggatctttt gggagtgatt cgacctgaca tcagcccaag
     6001 agcgactgga catattccgg aacagctgga agccattgaa aaattgcttg aacatggcct
     6061 ggcctatgaa tccaatggat cagtttattt tgaaataacc aaagacccaa agtacggtga
     6121 actttcaaac cgtaaagttg aagaaatgaa gagtggtgcc cgggtaaaag ttcgctctga
     6181 aaagcgtcat ccgggtgatt ttgccctttg gaaacgagca gagcctggac acttgatgcg
     6241 ctggcgagat ccatttagtg gctggggata tcctggttgg cacaccgaat gtgcggtaat
     6301 gagtacccgc tatttgggtg aagaatttga tatccacggt ggcggaatgg atttaaaatt
     6361 tccgcatcat gaatgtgaaa tcgcccaggc gagaggactt gaaaaaccat ttgcgcgtaa
     6421 ctggatgcac acaaatatgc ttaccattga gggacagaaa atgagcaagt ccagtggaaa
     6481 ttttgtaacg ctgtttgatg tttttaaaaa atatgatcca ttaactgttc gttactttat
     6541 tgcggcaagt cattatcgtt ctgtagtgga ttttagtgaa agcgcattaa cttcagctgg
     6601 attatccatg aagcgccttc atcaaactgt ccggaatctt cgtgaaagaa aagcagatgg
     6661 agcaaaacct gccggaaaat attttgaaga gtttcgcaaa cgtttttgta aggcaatgga
     6721 tgatgatttt tcaacacctc aagcgattgc agtgctgttt gatttgagtc gtgatgttaa
     6781 tacactcttg tctgaaaaaa cagctgaacc tgaagcaatt gttgatgcag agcatttgtt
     6841 ttccagcttg ggtggagatg ttctcgggat tattccggag tcgctggaag catctcaaac
     6901 cgaacttcaa ataaaaaatg taatggaagt tctggttgaa ttacgtgaga atttcagaga
     6961 gaataaagat tttgcaagtg cagacctgat ccgggagcgt ctacagaaga ttggcattga
     7021 attcaaggat ttaccggaag gaacaacttg ggaagtgact gataataaat aaatctgtaa
     7081 tctgtaacaa aaatttttta agcaaagttt tttgtcatga tggacaatca atcccgtcac
     7141 attgcctggc gactgtatga acttattgcc ccactcaaag gctgggtatt tttcagcata
     7201 gtttgcatgg tgggttacaa tatatttact gcggcacctg catattacgc caaagatatt
     7261 gtagatgcta ttgcctatgg cgacaatccg gaactaaaac agtattttct ggttggcttg
     7321 ggaatgatta tagtttttgc aatcaagggt ttgttttttt ttggtcacaa ctacagtgtc
     7381 ggacaccttg tacagagcct cattgtaaaa ctcaggcagc agctttttga ccatctagtc
     7441 aatctttcac tcaccttttt taaccgttcc aaaactggtg acctgatttc acgcttcact
     7501 aacgatttac atgtatttca aaacatgctt caagttggag tcacaggacc ttttcgtgac
     7561 attccacaat tttttctact gctggccatc atgttttttc gcagttggca gttggccctg
     7621 gtaaccatga caattattcc tgttgcaata ttttttatcc agatttttgg ccagcgcaac
     7681 aaggttgcag taagtcaaag gcaaattagt tttggagatt taagcagttt gctggtggaa
     7741 acaatcactg gtgttcgtgt tgtgaaggcc tttggaatgg aaaaatacga aagcaaacgt
     7801 tttaaagacg caaacgatga tctttacaaa aatcatatgc ggtcgatcat gattgattct
     7861 tactcatatc cggttattga aattattggt gctgcagctg gagcaacaat tgtggcttat
     7921 ggtggttatt taattattaa cgatcagatc actgcgggag atttcacttc gtttgtgatt
     7981 agtttttttt tgctgaatga accggtaaaa aaactgaatg gtttcaattt aaaactgcag
     8041 gagggaatcg ctgcagttca gagaattttt aatattcttg atgttaaaga tgacattgtt
     8101 atttctcctg atgccactaa actgaccagc tttgatcgtg aaataaatct aaatatacag
     8161 gctttccact atccggacca agatgaacct gcggttaagg attttcaact tactttgcaa
     8221 aaaggggaag ctgtggcttt agtcggcagc agtggtgccg gtaaaacgac cattgctaat
     8281 ttgatcccgc gtttttatga tattacacgg ggagaagtct acatagacgg acatgatctt
     8341 cgtgatttgg atatagcctc cctgcggaaa ttgattgcaa ttgtgactca agataccatc
     8401 ctctttaatg acaccattgc cagtaacatt acctatggac agccggattg ctcaaaagaa
     8461 cggatgtatg cagcggcaca agctgctaat gctcacaaat ttattttaga acagtctgat
     8521 ggttacgaaa cagtgattgg cgaaaaaggt gcaagacttt ccggtggaca acggcaacgc
     8581 ctttctattg cccgtgcttt ggtcaaagat gctccaattc ttattctgga tgaagcaact
     8641 tctgcacttg attctgaatc tgaaattgag gtgcagcagg ctattgaaca tctaatggaa
     8701 aatcgcacta ccattgtgat tgcacatcga ctttcgacca ttcggaacgc tgacagaata
     8761 tgtgtgatgg agaatggaca gattgtagaa caaggtactc acaatgaatt gctatcgaaa
     8821 gaaggtcgtt accagcaact ttatgaaatg cagttccagg atcaaccaaa tggttcagtt
     8881 tctgaagaat ccaaaagtcg actggtgtcc tgaattacaa tgaataaatc cccattttca
     8941 agaatcaata ctgccgtcat acttgcagca gggatggggg ttcgtttgcg aaatgtgatt
     9001 ggagtatgtc caaaaggatt gttggaaatc gagggaaaat ccctgattat tcgctcactg
     9061 gaacgcttaa agagtgaggg tattgatcgg gtcataattg tgactggctt ccaggataca
     9121 atgtaccata aacaccttga gcctctggca aacttgccaa agattgaatt tattcacagc
     9181 ccgcgttttg ctgaaaccgg aagtatgcac tcgctttttg tagcacagga agttttgcag
     9241 gatgattttt tgttgttgga gtctgatttg ctttatgaat cccgtgcttt gtcctcagtc
     9301 ttaaattatg agggtccgga tgtggtgctg gcctcaggaa aaacaggttc caatgatgaa
     9361 gtatacatct atggaattaa tcaggggaaa aatcataatt atgagacctc cagcagcatt
     9421 gtcagcggag atattgcggc tatttcaaaa cagcctcgtc cacatttaca gattcaggga
     9481 gaattagttg gaatatctat aatatctcag aatttgttcc gccgaatgtg cgctctgcat
     9541 gaagaaaacc tcacatttcc gtgcagtaac cattatgaag aatgtatttc agaggtaagc
     9601 tcagaatggg tggttcctta cttgcgggtc ggtgatttag tatggactga aatcgatgat
     9661 caatttcatt ttgaccgggc aataaaaata atatatccca gaataaaaca acaggaaacc
     9721 tcaaataaga caggattatg agtgattcat acgtattaca gcaggcaccg tttgtggttc
     9781 caacaactga cggaaaatta attgaagaac atgttggacg tgctgtgagt aatgaacccg
     9841 aagtgagtgt tgctcatatg gttgcacctc ctggttggag cgaaccacat caaaatccgg
     9901 agtttgatga atacaccatg atggtccggg gacgtaaaca aattgagatt gacggtgaaa
     9961 ctgttacgct aaatgttggt gagtctttgc ttgtccgcaa aggcgccaga gttcgttatt
    10021 ctaatccttt tgatgtagaa gcagaatatt ggtcggtctg tatgccggct ttttcacctg
    10081 atttggtgaa tcgggaggaa gattcaggaa gctgaacaaa tttcatcgac tattcgggcg
    10141 gcctcataat ctgacgcttc agaaaaacta agtacctgct gacgcatatt atggaattca
    10201 gagtcagaaa gtgccccttt tttcataacc gactcaatca catttcccaa atcctccact
    10261 ttacataccc catctgcaaa atcatccctg aattgtaaat cggcaccacc ttcatattca
    10321 gcaggtgttt tcaggaaaat gacccttttg ccatatccca ggcggtattc tccacctaca
    10381 ctgccaaaat ctgttattaa cagtgaggat tcttcggcta aattttgaat accggacaat
    10441 tcatcaaaat gaatatgctc aacacctttc agctcttgac acataaatgc agtaacataa
    10501 tgtctccgtg aggcaagtga gggatgcagt ttgattacaa aattgtaatg ggacatctta
    10561 ttaaaaattt cgagaagccc cctgtccagt gcgtcaaaca gtaagctaag ttccaatgaa
    10621 ggtgcataaa gcacaactgg attttcagaa tcagggaaac agcttacagg gctggaagca
    10681 gcctgcagtt ttggataacc cacagaatga actttgatct cttcctgaaa cttttgctcg
    10741 agctgcttgt aagtgtatgg cggtcgagtt ggaagaaaaa catggttgaa gcgaatattc
    10801 tccggaagcc caatatcaat tgctttgggc agagcataat gcggcaggta aatgcgttcc
    10861 ggaacagaga atgtttcctg cctgattgac tcacaggtta catgaacatc cgcgtcggga
    10921 aaagatgtgt attccgataa ttcctcatgt gaaaaatgcc gtaaaggtga aatgtttagt
    10981 tgcagcagac gttcttcgac ttgttggcga aaatgaagaa attcgaatcc tttaccgatg
    11041 cgatgtagtg ttgtggaaaa atcaatgtaa aaaacctcat atttcccggg aaaagagcgc
    11101 tcaagcgcta aatcaattgg tgcaaaccaa tccagaaaat gaagagtgcg aaaagtgaaa
    11161 tatattattc tacgtccgga agacttatca agcagggttt gtttttcttg ccgtaaataa
    11221 ggttgggcct taaatagtga atcgcctttc agcagcaggc gccgtattaa aaattccagt
    11281 atcttttgaa ttattttgtt ccagtgtaac tgtaggcgta ctccctgaaa gcggagcctg
    11341 aacaataaga gctttccttt tgagacgaac gaactcaaga ttatttttgt tccaaaattt
    11401 gaaggactct ttcacccatc tcctggcaac cgatgagtgt tttcccttcg gccataatat
    11461 cgtcggtgcg atatgtttca agagttttta cgacagcctg ctgaatcatt tcatcggctt
    11521 cagtgagatc aaaagaatag cgcagcatca tacccactga aagaatcatg gccagtggat
    11581 tcgctttgtc ctgtccggaa atgtccggag cagaaccgtg aacaggttcg tacattccgt
    11641 ttttcccacc gatacttgca gaagggagca ttcctaaaga accggtcatc atggcagcag
    11701 catcactgag gatgtcgcca aacatgttgg ttgtcaccat ggtatcgaac tgctttggat
    11761 tgcggataat ctgcatggcc gcattgtctg cgtacatgtg actcagctca acttccggaa
    11821 agtccttgcc tacttccgtt gcaatttccc gccacaattc agttgattca agaacattag
    11881 ccttatcaac agaggtaagt tttttcttgc gcttcatggc aatttcaaag ccgactttca
    11941 caatacgtcg aatttctgat tcagaataaa caagtgtatt aaaaccaacc cgttcgccgt
    12001 tgcgtatttc cacaccacgt ggctctccaa aataaatacc tccggtcagc tcacgtacca
    12061 ccatcaagtc tgcgccatca acgatttccg gctttagtgt tgaagcattt accagatcac
    12121 cgtatattac agcaggccgt aaatttgcgt acaattccaa ttcggaacgc aatttcagca
    12181 aacctcgttc aggacgtacc gaaaaatcaa gtggctccca ttttgggccg cccactgccc
    12241 ccagcagaac tgcatcagaa ttttttgcgg cttctagcgt ttcatccgga attggggtac
    12301 cggttaaatc gtaagcgact ccaccaacag gtttttcaga taattcgagt tgaagttcaa
    12361 aacgtggtgc tacagttttt aacacatgaa tagcctgttt tgttacttcc ggaccaatcc
    12421 cgtctccggg ggtaacaagg attttcttag tgatcataat acctttttta gtgtgaaaat
    12481 ttctactcct gaaaactatt aatcctaatg agaagctgtt gctaatgcaa gaaaaaatgt
    12541 tcagattgat gcttggaacg ggaattatta ttttgttatc ttccgttttg tgggcagcaa
    12601 atccttctca atctaagttg gatattcagt taaaatctct ggtccgtcac ggaagcgtat
    12661 tgatcgccaa tgagcaacag gtgctctatc gctatcctcc aaataaaaac ccgttgctga
    12721 ttccagcctc agtgctaaaa tatgcaactg cactgtcagc tttgcattat tttggatcag
    12781 aatacagttt caatactgaa ttttatattg atcaaaatca ttcccttgtc atcaagggca
    12841 aaggcgatcc ttttttagtt tccgaagaat ggcagcgaat agcacaacaa ataagcctgc
    12901 ttcccgatgt ccctaaaaaa atgcagggct tgttttttga cacctcactc tttagtgaaa
    12961 aaattaaaat ccctggaatt tcgttttcta ataatccttt tgatgcacat aacggtgcac
    13021 tggtggttaa tttcaacaca gtgttcgtga aagttgattc aaaaggccat gtcacttctg
    13081 ctgaaaaaca aactccactg acccctttgg cacgtcgcct ggctaaaaaa ttgtcaccag
    13141 gaactcatag gatcagtata caacctaact taagttttac ttattcagga gaacttataa
    13201 aatcattttt tcgcaaagaa ggattttcat ttgaaaacaa aaacgttgct ttacgttctg
    13261 ttagaaacgg agatcacctg ttttacactc acagcaatat tttaacattg aaggaagtaa
    13321 ttgcgggcat gctgcgcttt tccaataact ttacggcaaa tcagctttta ttaaccgttg
    13381 gcttgcagcg ttacggccct ccggccactc tggaaaaagg aacacgcgca cttacagaat
    13441 atttacgaca agaagtcaaa ttacccaaag aacaatttaa gttggttgaa ggttcaggaa
    13501 tctcgcgcaa aaatcgtcta acaccggaag ccatttggca gttgctaaag gattttgctc
    13561 cgtatcagga tttgcttcac gaagataatg gtgttttact aaaaacgggt accctcagag
    13621 gggtttacac tatggccggt tatctgcccg gcacgcaacc gctctatttt gcgatcttgc
    13681 ttaatcagtc tcaaaattat cgcaacaaga tactccagat tctcttatca acagattttt
    13741 ctgccggcaa attttgaaat ccaacccgat aatttctatc ctgatgccgg tacgtaacga
    13801 tgctgcaatg ttgcgcaact gtcttactga cattcaaaac cagagtttta caaattacga
    13861 actagttgtt gttgatgacg gttcagccga tgaaaccccg gtactgctgg aaaaagcaag
    13921 tcaggatgat tccagaatcc ggatgattcg cacagaaccg cagggcattg tttcggcact
    13981 taacactgga cttattgaat gtaaaggaac ttatattgcc cggatggatg tcgatgacag
    14041 gatgcagaaa acaagattgg aaaaacaact ggaattaatg caaaaagatc ctgaattgga
    14101 gttgataggt tgcagggtgg aaggttttac tgataaagga ccgcttccgg aaagcgcagt
    14161 tcattaccag tcctggagca attccctgat gtctcatcag caaattgaat gcgatttgtt
    14221 tgcagaatca ccaattgttc atccgacttt ttttgcaact cggaaattat tcatcaaaat
    14281 tggcggttac tcaaacaatc cctgggcaga agattatgat tttattttgc gggcatacgg
    14341 cggcggagca aagttggcta aacatccgga aatactggtc cgtaaatatt ttgcccccgg
    14401 acgtctctca agagttgaag caatgtataa acgtccggcc atgtttgacg caaaagcaca
    14461 ttatctccta gagtttggtc tactcaaaaa acgtcgtggt gttctaattg taggctcagg
    14521 tccaaccggc agacaggcgg cacaaagttt tgaaaacaga ggggttcgtt tgctgggttt
    14581 tgtggataat cgtccggggc cacctgaccg tcaggtgaaa aaccggccgg catggggttt
    14641 taataatctg cctcctgagg aattcatgca taaattccgg gactctttga ttgtgctggc
    14701 tattggagac tccactggtc aaagagtttt tgcagaattt ttgagaaaat cggattttgt
    14761 tgaaaaccat gattttgtgc gtgtgattta taactggcct cctgtggcta actcaatttg
    14821 actgttattt cgtgttcctg attgtgaaga ttcttgtttt gaagtaaaat aaagttctct
    14881 gtctaccagt caactttgcc ttaaatgagg tggatttctt tcaaaaaaat atataaatga
    14941 ctctgtcgaa aataaatttg cttcggctgt tttttttgct ctggctgctg gtttttgtta
    15001 ctccctctgc acagagcaaa caggcgccac tggctggtga agaacgaaaa ttactaaaca
    15061 gtctgcgcat tactcctgct acagcctgga ttgaggcaca taattttgcg ggaaaacttc
    15121 ccgatgaaaa gacagtcaat ctgaaagact accgtggtcg atttattttg ttgaattttt
    15181 gggcgacctg gtgttcgccg tgtctgaaag aaatgccgga ttttgagaag gcatatcagc
    15241 aaatggggca ggaaaaattg atcgtgctgg cagttggaat gggagaagac acgaaaaaaa
    15301 tcagcaagtt tgctgagaaa tacggtttca cgttcccgat ggtagcagat ccaaagctgg
    15361 aaattaccaa cttgtacggc gtcaaaaaca ttcctgttac ttacctgatt gatccggaag
    15421 gagtagtttt gggaagagca ttaggtattc gggattgggc aaatccggat ttactcgcct
    15481 ttataaaatc ccaacttaaa taaacaaaaa caacacgaaa ttttgcagat ttgctgttga
    15541 gaatctgtca gaaaaaataa ctgaaaatat tggttcttgc attgattttc tgtggtaaac
    15601 tcagtataat tcttgaatag agttttttct tgaaattata caaacagatt atggcaggca
    15661 ctaatcctta tattgagaaa gcggaatttg aattacctct tcaaaactat gatgtaacgt
    15721 ttctgccaat gcagaaaaag gtggctgttt tatctgaaaa aattacaact gaatccgacg
    15781 gacttccggg ttcaattctc aatgcagccc tggaagcagg tatcgatatg gaccattcct
    15841 gcggaggcgt ttgtgcctgt tccacctgcc atgtcattgt acggcaggga cttgaaagct
    15901 gtaacgaggc tactgatgat gaggaggata tgctggattt agcacccgga cttgaacctc
    15961 agtcacgtct tgcctgtcaa tgcgtaccta atggtaccca ggctctcatt attgaaatac
    16021 cggaatggaa ccgaaacctg gtcagtgaag accactgacg ctggagaaac tatgataaca
    16081 cttacagaaa atgcggcaaa ggaaattcag aaaatcatgc tggaaaatga attggcagaa
    16141 aacgtttgtg taagggtagg ggttaaaggc ggaggttgtt ccggatttac ctatacactg
    16201 gatttcgatt caaaaaagac aaaatttgat ttagagtttg aatcacaaaa tttaacaatt
    16261 ctggtcgata aaaaaagcca tctatacatc aaggatacag aaatagactg gagctacagt
    16321 ttgatggatc gtgggcttaa attcaacaat ccatcagcca aaggttcatg tggctgcaaa
    16381 acttcattta tgtttgaacc accggaacag gaagaagatt ttaaaccaag ttggatgtaa
    16441 atttaatgca ggaggattta tgagtttagc tggaggtcag aaaactattg aagaacaaat
    16501 caaggaggca attgaaaaat ctgttgcaga ctcaactgct gaagtgggcg gggacggacg
    16561 gcattttgaa atccaggtaa tttcacctgt ctttgaggga aaacgtaccc tcgagaaaca
    16621 gcggattgtc tatgcagctc tcaatgacct gatgtctgga agtaatgctc cggttcatgc
    16681 gattgcccgc ctagaaactc tagtgcctga ataatttcgg atacacttgt agctattcaa
    16741 actttgttga acatgaaaat tgaagaaaaa cgagtaactt ttccgaagac tttatcagtc
    16801 aaatatgcag gtaaaattgt tgaagaaaca gctactcacc tgactctgga gggcgaggat
    16861 gaagattcgt acctaaaaat ttttacacct tttcgagggg ttgccaaact gctgctgttt
    16921 gaaaatgata agtgggtgga tgcagaaaac tcatctgcag tttccaattt tgatttttct
    16981 tcactcggac tgggagatct ttcaacttta tctgccggag atgaagcaga ctcttcggga
    17041 acaaaaaaat gactctagtt gatgaattga tataaagcaa aaattgctta aaaagtattt
    17101 aaccacagag tcacagagaa cacactgaat tgtttatatt ttatactcac agaggcctct
    17161 gtggccccat ggtgacggtt tttacgatac tgagctattt tcgaaaccac cttatttttt
    17221 aacacacaaa tagaaaatga caatgagtga aacaaaaatt tctgacggac ttccgcaaat
    17281 tcaaatccag accgatcgcg gcaatatgac aattgaaatg tttgaagacg atgctccaaa
    17341 cacggtggcc aacatgattt ctttgatcga aaaagggtat tatgacggac ttaatttcca
    17401 tcgtgtaatt ccggatttta tgatccaggg cggttgtccg caaggtaccg gaaccggtgg
    17461 ccccggatat gattttgatg atgaatgtac accggaacgt cggcatgaca gcgccggaat
    17521 tctttcgatg gccaacgcag gtcccggaac caacggaagc cagtttttca tcacacacgg
    17581 ccccaccccg catctggacg gtaagcatac tgtttttgga aaagtcactg aaggcttgga
    17641 agttgtcaat gaaattaaac aggatgatgt gatggaaaaa gttttggtgc ttcaaaaacg
    17701 cgatcatgtt tatgaagtag aaacccttta agggctgatg aaaattagac tgatcctgaa
    17761 gatgctgaaa aatgaaatat ctgatagttt tagaagaaac tagaaccgga ttttctgctt
    17821 tttctccgga tattgataga tgtgtcgcca caggtgcaac caaagaaaag gtagagaaaa
    17881 atatgttgaa gcacttgagt ttcatctgga aggattaaaa atgggagacc aaactattcc
    17941 gatgcctcat accttttcta tttatcttga aattcaagtt gtgtcctgaa aactaaactt
    18001 caaaattaac tatttttgaa ccatgagcga aacaatactt gaagcagata tagtccacga
    18061 aatgcgtgac tcctacatgg agtatgctat gagcgtcata gttgggcgcg caattccgga
    18121 tgtccgtgac ggcttaaaac ctgttcaccg gcgtgtactg ttttcaatga gtgaacaggg
    18181 taacgatttc aacaaacctt accgcaaatc tgcacgtgta gttggcgatg taattggtaa
    18241 atatcatcca catggagata cggcggttta cgacacaatc gttagactga cacaagattt
    18301 ttcgatgaga tatcagttga ttgacggaca gggtaacttt ggttcagttg atggtgattc
    18361 tcctgcagca atgcgttata cagaagtccg catgcggaaa atcgctcatg atatgctggc
    18421 agacctggaa aaagacacag ttgattttat tccaaattac gacgactccc tggaagagcc
    18481 gacagtcctg ccctcgcgta ttccaatttt gctgaccaat ggttcgaccg gaattgcagt
    18541 cggtatggcg agcaacattc ctccgcataa tcttaaggaa gtgttgaatg cgttgttgtt
    18601 ttatattgat caccgcaact ctgccacagt ccaggaattg atggaattta ttcccggccc
    18661 ggattttcct accggcggta ctattattgg taaaagtgga atttatgacg cctacacaac
    18721 aggacgtgga atcatccgga ttcgtgccaa aagtcatttt gagcagacta aagatggacg
    18781 tgacatgatc attgttgatg aactaccgtt catggtcaac aaggcaagtt tgctggaaaa
    18841 aattgcggaa ttggtgcgac acaaaaaaat tgagggaatt tcagatttac gggacgaatc
    18901 tgatcgcaag ggaatgcgca tgtttattca gctaaaacgt ggtgaaactc cggatgtggt
    18961 tttgagtaat ctttacaaac acacagcact gcaatcgagt ttcgggatca ttatgctctc
    19021 cattgtagac aagcagccca aaatcctggc cttgacgcaa atgctggagt tttttctgca
    19081 gcaccgtatt gaagtagtaa cacgccggac acgttatgaa ttaaaacagg ccgaaaacag
    19141 gatgcacctg cttgagggtt tgatgattgc cctggaaaac ctggatgcag tgctgaatat
    19201 tattcgaaaa gctgaaagcg gcggtgtggc agaggatgtt ttgatggaaa gctatggtct
    19261 ttccaaacgt caggcccatg gaattctcga tatgaaattg cagcgcttaa ccggaatgga
    19321 gcaagataaa attcgcacag aacatgaaga tgttggcaaa gccattgagg attacaagga
    19381 tatcctggaa aaagatgagc gtgtcatcga aattatccgc aaagagagta ttgaaattcg
    19441 cgacaaatat ggagacgagc gccgtacaga aattattgaa ggtgacagtt caatcctgat
    19501 tgaggaactg atcaatgaag aagacatggt ggtcactctt tcccgtgaag gttacataaa
    19561 gcgcagttca gtcagtgagt acagactaca gcgccgtggc ggaaaaggac gcctgggaat
    19621 gggtacgaaa gatgaagatt ttgtcgaaca gatgtttgtt gcctccacac atgatttcct
    19681 tctctttttc actgacaaag gtcaggtttt ccgcaaaaaa gtttttgaac ttcccctggc
    19741 cggaccaacc ggacgtggaa aagcggcaat taatttgctg cctttaaggg agggtgaatc
    19801 aatttgttcc tgtctgaatg ttcctaaaga agcagaaaat aaatatattt tcctttgcac
    19861 agaaggaggt gtagccaaaa aaacaccaat gctggattgc gcaaaaatcc gagttaccgg
    19921 tttgcgtgta attactctcg acgagggaga ttcagtgatt tctgttcagc taacagatgg
    19981 aaatcaagaa ttgttttttg ccacacgtga cggtatggga ttgcgtgtta atgagtcaac
    20041 tttacgagcc atggggcggc aggcgcgtgg tgtgagaggc gtcaaacttc gcaaaggcga
    20101 cagggtggtt tctgcactgg cactttccgg tacaggtgaa ctgctgacgg ttacagaagg
    20161 tggttacggc aaaaaaacgc cgatagtaga ttacactctt gcaaaaaaca ggggcaactt
    20221 tggaatgcgt acaatccgga ttactgatac aaatggtccg gttgtgaatg tactggaagt
    20281 tgaagagcat tttcagcttt ttctgatcac tcagcttgga aaattgatcc ggattcctgt
    20341 tgaaaatatc cgtaccatcg gccgcgttac gcagggtaat cgtctgatcc gccttgagac
    20401 ggatgaacag gtgatcggca ttgctccggt gattgaggat gaggatgatg acgaggttga
    20461 ggtcaaagag gaaaattcaa cagactcaac aaccgtctca gcggctgaaa ctgctccgga
    20521 aaacttggtg gaagaaaaca ttccagagga accacaagac ccttctgact ccgacgaacc
    20581 ttccgattca tgactgcttt tctatagtgg ctcattttct gcatttgtaa gttggtgtct
    20641 ctaccaccgt agtgctggtg tgagactcca catgcacctt tttccactgc aatttcatga
    20701 agcagttttt ccttttatta gtactcagtc tgctgttttt atttccaaca gcagggttat
    20761 ctctctccgt aagttccggt catatgcttg accgcttcga attgattccg gatgagaaaa
    20821 cgccatttct gacctttaaa ggttttttcg atccaggaca atttcctcat atccagattt
    20881 tcccaagaaa ttccaagacc gaaacttccg ttcttctgcc caacacattg atcaacagca
    20941 ttctcctgcc ggaacgggaa gtaacttcct tttcagagga gggtatcatg gagaaaatgc
    21001 tacttgagga gaaaatcaca aaaaaagaaa atggggaaat agagttccag gtgaccctca
    21061 ctatttcaag tactgaagaa catattctgg tgctggatac agaaaaatca gattcacgtc
    21121 atttaacatt tgccttgcaa aagcacgtaa aaaaagaaac cacaaatgct caagaacagc
    21181 agaaagtgga aagtataata attgatttgg caccgcctgc gattagtccc cgagaggaga
    21241 tgcttttgca tccagtgacc gcactgatgt cgtaccgccg tgttgatcaa ctgaatctta
    21301 cgatactgaa cgcttctcct catccggact cggcacagcg tcttgcagta ctgttggacc
    21361 gtcaacagaa acgtacgatt gaaaagcgga ttggaatgaa gttaaaaatc gttaacatat
    21421 cttcggtccg tgaacagaca atcctgccca aaaccaaaat ttactttcat gcaaatttga
    21481 tgagagaggc cttgattttg gcgcagattt taccgggaga acaggtgatt gtaccaatgc
    21541 cgcagagtag aataagtaaa ctggcaaccg atgtagaaat ttatgtggga aagaattttg
    21601 aataaaaaaa tatacttcat ctctcttgca ttggccctgt ttgtgaccgg tttcagtctg
    21661 ccggcatttg cctttccgga atatacgtca cggcaaaaag cactcaaact ggcaggtgtg
    21721 ctggatcatg gatatttcaa atccatcaaa atttcttctg tttttgtcaa aaacaggggt
    21781 aaagacgact attatctgca ggcaattctt gatgacggtt ccagccgaaa gtggttgata
    21841 aatcgtattc gcgaatgggc acagactgat gaactgattc tcagtaaaaa tcgtgcactt
    21901 gtctttccta caccggaaag tacagatttt ggagtacttg acaagaataa gttttaccgc
    21961 atcattttaa actcaactgc ctttgtcaaa acatttggca aacatgacat cctggacggc
    22021 aagagccttg ttttgggaat tcgccgcttt cgcattctac aggcagaaga tagtaaatac
    22081 atgaaaaccg ataaatttgg tgatcgctac cgttatgttt tggaattaga gaacggttca
    22141 cgggaattct tcacttacag tgatgctttc ctgtttctcc agcgcaccgc attattagag
    22201 aaggctccgg aaaatgtcag tgttttacga aaaactttta aggtccgcga actgaaaaaa
    22261 attccgctgc aaattgaaga tgagttacgt gaaattaggc gctttggaat tgaggtggtt
    22321 tttgatggac caattttaat gagtcctgac cgatttccgt ttcaggttgt tgaacagaaa
    22381 atgagggatc ctgagacagg aagatacaag gcccagtttt tcctgcagat tatttttcca
    22441 aactccgaaa aaatccgtga gattccagga tttaataatc atgagtattt acgttatgtg
    22501 gaaattgata ccgatgtgga gcaccagaaa agagtaattc tgagtgcaat ggtaaatccg
    22561 aatgcactgg atttgcctcc gtacattgag gttacagaca gaaactcggt tgtggtgaat
    22621 tttttcagtt caacagacca gagtttgata cagccccctg acttactggc atcacactct
    22681 gcaggagaat taccgcgctc agttttcact ccggatcaac ttgagacaga atttgagaca
    22741 cactacatgg aggccgtcgg tctgatccgc accgcacaga atgagatgaa tatgagcgtc
    22801 aagattgaaa cctattttaa agccctggac actctgaaaa aagctgcact cacagcagag
    22861 tttgacatcc agatagcgca agccctgaac cagcgcgata ttctgctgct gacgatgccg
    22921 aaactgatta ttcgaaatgt gcagatgaca attctggcac ttcagcttga tgatctaaac
    22981 ataaaacctg atcctgtttt gactgttaaa ctgctaaagc aattaaacca tgcggagaac
    23041 tatgcttcta cgcaggatca acgacagaaa attgcctcct tgcaaagcat tttacgctga
    23101 gcaaactaca gactcctcca ttgccatttt taagctgctg tgttagactg tgcagacagt
    23161 cgaggtgtgg cgcagtctgg tagcgtgctt cgttcgggac gaagaggccg gaggttcaaa
    23221 tcctctcacc tcgacca
//
LOCUS       DTVD01000178           23223 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_329_length_23223_cov_305.703988, whole genome
            shotgun sequence.
ACCESSION   DTVD01000178 DTVD01000000
VERSION     DTVD01000178.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 23223)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23223)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23223
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_23223_cov_305.703988"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            201..728
                     /locus_tag="EYO68_06265"
     CDS             201..728
                     /locus_tag="EYO68_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIC07444.1"
                     /translation="MSLLSIFLMDEPIQIEPIRRLPHIRDLVTDVSWNYEINQHIRPL
                     KPKPREADGTYRMQQKDIERIQEFHKCIECFLCQNVCHVMRDQQVKEFAGPRFLIRIA
                     SLAMHPLDTLNRLKELKDVFGIGYCNITKCCTEVCPEDIAITDNAIIPLKERVAGAFY
                     DPLAWLWRSLTSVSK"
     gene            815..1474
                     /locus_tag="EYO68_06270"
     CDS             815..1474
                     /locus_tag="EYO68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate hydrolase"
                     /protein_id="HIC07445.1"
                     /translation="MKTAPNYLNPEIPSGLKRVTIPIVEATAESLAGYGHIVSDTDEV
                     EIEIVRWPAQGHREVDPDSGDEGGTTEGLFICEWKGDILYGRNSAVSGHYILGYGTEP
                     KKADEHHTRDPKTLLVWHANYHPDGGQCFFPETKKPFVVPLALPGDDISPEDFVCFHF
                     SGHEGLYIHPNVWHEGALGISGEQRFFDKQGAVHARISVDFAREFKCLLEVSLEQFNP
                     A"
     gene            1525..1947
                     /locus_tag="EYO68_06275"
     CDS             1525..1947
                     /locus_tag="EYO68_06275"
                     /inference="COORDINATES: protein motif:HMM:PF06983.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIC07446.1"
                     /translation="MEKFAPYLMFNGNCEEAVNFYKECFNGEIGLMGRYGDSPMEVPE
                     SRKDKIVHVELKFWGGSILASDNMDSTESSKTDSSCVHLSLGFEDPLKMERTYEKLKQ
                     DGQPTMPLKEQFWGDKFGMLTDKFGIKWMFNCPKKESA"
     gene            2052..2783
                     /locus_tag="EYO68_06280"
     CDS             2052..2783
                     /locus_tag="EYO68_06280"
                     /inference="COORDINATES: protein motif:HMM:PF02146.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07447.1"
                     /translation="MPDKLPREFTLSGLREGRSNVTVITGSGIDVESGLPSFRGEKGY
                     YEDKETVYLASVDALNSEPIRQWRWYLKRFVSYHATPPAASHFSLAELEAEIGEKFNG
                     IVTQNISGLHRKAGSQKVFEIHGCIREMRNLKTGERRPLPQSWVESPPEDEELCGWRP
                     HVCFIGESYDDYPLPESVSACSSCDILLVIGTAGVIHTPVWLAEQARLSGAVVVNINP
                     NPGELDNVSEFSFRGTSSEYFHYDI"
     gene            3331..4014
                     /locus_tag="EYO68_06285"
     CDS             3331..4014
                     /locus_tag="EYO68_06285"
                     /inference="COORDINATES: protein motif:HMM:PF02493.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Tir"
                     /protein_id="HIC07448.1"
                     /translation="MKNILIILQITCVCLSTISCATKSNDSAKGVFFYRESFGEWGWH
                     EDGDEEKDGIYEGETRKGKPDGQGTYTHYSETKSSVLLLPVMIPGWGESIVFALIIWE
                     MLHQGQPKIQDAKYVGAWKRGEKHGDGTYYFPNGDRYVGEWRRGGKHGEGVYSFSDGD
                     RYKGNWRDGRKHGQGTYTFSDGDIFAGEWKDGKVHGNGTYTYPDGSKLIGKWKDGKKN
                     REGKLILSD"
     gene            4192..5268
                     /locus_tag="EYO68_06290"
     CDS             4192..5268
                     /locus_tag="EYO68_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07449.1"
                     /translation="MEQKFSVLLLFTLFFTSIFFAVPFFSTSILAQSKETGVLYLWEI
                     SSGKVWKSFGDDETQQKYKGEIKEEKPEGLGVTLFSDGTKYMGQWHDGMQHGQGTFIF
                     TSGEKMTGEWKENAEWNITKYDINGKIIAKYADGVLLIDNKKEGVLFFRQEFGSMEWF
                     EAGDEEQDYIYEGEIENDKPNGWGTFTYPSGSIYEGEFKEGKFHGQGTFTSVEGKKGV
                     GEFKENKPWNVTEFDKDDKIISRYTDGVEIIEKKLVGVLFTRKEKGKWIWYENGVEDE
                     GGKYEGEIENDRPNGKGTLIYRNGTRYVGEYTDGAWNGMGTFYFPDGEKWVGEFREDA
                     PWNINWFDRDGNIIAKWGNGVKLK"
     gene            5415..7817
                     /locus_tag="EYO68_06295"
     CDS             5415..7817
                     /locus_tag="EYO68_06295"
                     /inference="COORDINATES: protein motif:HMM:PF08238.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HIC07450.1"
                     /translation="MSYLKTQKNIMSQNIVPVFPVYFMRGLLKRIFNTIFLCLFLPVF
                     SFAAGNFQKGYEAHLRGDFSAAIKAWGPLAAQGDAAAQFNIGNLFDFGKGVREDNVQA
                     AYWYNKSAEQDNANAQYALGWMYENGEGVKKDLVKALLWYKKAAEQKHSGAQTNLGWM
                     YENGKGVELDHTQAVFWYKKAANLGRAEGQLNLGIMYKKGLGVDLDQSKAVFWFEKAA
                     SQGYANAQLNLGVVYRNGEGVETDYIKAAYWLKKAVKTGHSAAQTNLGWLYENGKGVA
                     QDYQHAFSLYQKAAEKGQAEGQLNLGVMYANGKGIKVNYKKAVYWYRRAADQEQAEAQ
                     LNLGIMYKYGRGVEENYTQAVYWFKKPAEKGYAEAQTNLGLMYEYGKGVKQDYTEAIL
                     WYEKAAEQDDVTSQLSLALFYKFGKGVRKDYTQVFYWYKKAAELGDTGAQLNLGFMFQ
                     YGKGVEQDYEEAVTWYKKSAERGHPEAQASLAWMYENGKGIEADLKEAIYWYKKSAEQ
                     GNTKAQLKLGIIYKNGNGTVEDYTQSFYWYKKVAQQGDAHAQLNVGIMYEYGKGTTQD
                     YTNAVHWYKSAAVQGLSEAQTRLGWMHENGKGVEQDYSQAISLYKNAAKNGETNAQLK
                     LGIIYQNGKVAAEDYSQSFFWYKKVAEKGDANAQLNLGQMYEYGKGTDENYTQAVYWY
                     KKAAEQGDTEAQLNLAVMYKYGTGVNEDSSQAVFWYKKAAEQGNTKAQLNLAVMYASG
                     EGILENFVLAYKWANLAAARGNENAREMKNEVKKRMTRTQIAEAQQLSLEWQQNQSGA
                     EN"
     gene            complement(7896..8933)
                     /locus_tag="EYO68_06300"
     CDS             complement(7896..8933)
                     /locus_tag="EYO68_06300"
                     /inference="COORDINATES: protein motif:HMM:PF05036.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIC07451.1"
                     /translation="MNRKRFIRLLLLWLSTFLMGGIVSVLIYVQMAVNQENIVGSLEI
                     DSPEVEADSETIEPVSEEAGPEPKPGAVGTADISEDAEYSHLVETSKPFWNVFPINKP
                     GVSAENWHTILVQNRFKIDYTITPLSQRDWHLVRINNSIRVQFRSELAAIKDILYRKK
                     RGKFSVQLVSIEGGQFQLAVSLMSRLLHAGHYAYLHRTDAEFDGKFWYRVRVGFFKNL
                     EDALTTGKKIFEKFKDEELFSENFMPVQPGPQELSRPVIDLRQPLNKPWVIELPLYDV
                     QNKALKDLAELNTEIDFSYISQKLKSASSGKLAYRIRIGFFETKREAGGMIYRLKKKF
                     PKLKRMKRVHL"
     gene            9098..10342
                     /gene="lysA"
                     /locus_tag="EYO68_06305"
     CDS             9098..10342
                     /gene="lysA"
                     /locus_tag="EYO68_06305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIC07452.1"
                     /translation="MNIINDTLFLEDISVETLMQEYGSPLYVYEESVLRSQFRQLSSG
                     FPEGLLEIHYSMKANSNPSLLKILREEGSSIDAVSEFEVRLALESGFEPQQIIFTGNN
                     NSLAEIEYCVEQEVSVNLGSLVLLEKFGQRFPQTSVSVRINPGIGAGHHAHCITGGPD
                     SKFGIYHDQIDAALALATKYKLTLNGVHSHIGTGIYSAEPMLEAMEMILAVAEKFPDL
                     EFIDFGGGFGIPYRPEQQALDMKDLSQKMTRRFTEFRQCYGREISMKIEPGKLLVGPA
                     GILLTTVTNITDTPKHRFVGVDSGFNHLLRPTLYGSYHKILNASRYSGDEEDVVVVGY
                     ICESGDIFSRSGDEIERKLPSPVIGERLAFLDAGAYGFSMSSQYNSRPRPAEVLVHQG
                     QSRLIRRAETFEDLAQFFVDFN"
     gene            10355..12007
                     /locus_tag="EYO68_06310"
     CDS             10355..12007
                     /locus_tag="EYO68_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HIC07453.1"
                     /translation="MNKKKLGKQKGRLVDSEALLEVQKLLSDMPLRKDMLIEALHLIQ
                     DEFHYLSAKHLVALAHEMKLAHAEVFEVATFYHHFDVIKENDVPPPPLTVRICDSLTC
                     EMSGAKELTESLKNVLGEGVRIQPVPCVGRCQNAPVAVVGKNPLDLATIESVQNAVNK
                     KEVRPEIPEYVGFSQYRDTGGYQLFQECNSGKRTAESVIDELKNSGLRGLGGAGFPAG
                     SKWEIVKRFSGPRLLAVNIDEGEPGTFKDRFYLETDPHRFLEGSLIAAWAVGIDEIYI
                     YLRDEYAAANEILLREIKELQSAFPDILPQIYLRRGAGAYICGEESAMIESIEGKRGI
                     PRLRPPYVAEVGLFDRPTLEHNMETLHWIRDIVEKGAKSFSSHGRRGRKGLRAFSVSG
                     RVQKPGVHLAPAGISMLELIDEYCGGMLPGHSFYGYFPGGASGGMLPASLGDLPLDFD
                     TLQEHGTFIGSAAVIVFSDQDSARKLALNAMRFFEHESCGQCTPCRVGTAKAIPLMEQ
                     KTWNKSLLEELSQTMEDASICGLGQAAPNPLRCVMKYFPQEV"
     gene            12009..14759
                     /locus_tag="EYO68_06315"
     CDS             12009..14759
                     /locus_tag="EYO68_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIC07454.1"
                     /translation="METISFELNGQTLEALVGETILQAADRAGVADIPRLCYKEGYRT
                     DGNCRSCVVEIEGERVLAPSCCRYPQQGMKVNSKSERAVHSQKMVLELLLADMPESGK
                     SPYTPNSELDEWVEKLGLSKPRFSRRVQPAFDNSHPAMSVNLDTCIQCTRCVRACREV
                     QVNDVIGMAYRGSHAEIVFDFGDPMGDSTCVACGECVQACPTGALMPANDVGLAAPDK
                     KVESVCPYCGVGCLLTYHVKEGKLLRVDGREGPANHERLCVKGRYGFDYVHHPDRLTK
                     PLIRRKDAPKTIELLQPKDWQKVFREATWEEALDFAADGFSKIRDENGPDALAAFGCA
                     KGSNEEAYLLQKLVRTGFGTNNIDHCTRLCHASSVSALLEGIGSGAVSNPFTDVTDAE
                     VIFLIGANPIGNHPVAATFMKNAVKAGKKLILLDPRRTALSRHATYNLQFRPDTDVAL
                     LNSLMHVIIKEKLCDEKYITEHTTDFEKLKANVADYSPEKVAPICGIPEETLREVART
                     YATAESAIILWGMGISQHIHGTDNSRCLIALSLMTGQIGRPGTGLHPLRGQNNVQGTS
                     DMGLIPMFFPDYKSVTDPENKKWFENFWGTSLNPKTGLTVVEIVDAVHSGGVRGMYVQ
                     GENPAMSDPNLNHARGALSMLEHLVVQDIFLTETAGFADVIFPASAFPEKSGTFTNSN
                     RQVQLGRQAIDPPGNARQDWWIIQEVARRLGVDWSYDGPEEIFSEIRKATPSMAGITW
                     KRLQKEHSVTYPCNKEGDPGEAIVFRKGFPTANKKARFVPAKYTHADEMPDEEFPFVF
                     ITSRQLEHWHTGSMTRRATVLNAIEPYPVVHVHPDDLQKLGVEPGEAVTIESRRGKIS
                     TYSRQDPGVQQGNLFMSFGYNEAAANLITTSVLDPFGKIPEVKFCAVRMSAGGNPEMR
                     IG"
     gene            15218..16258
                     /locus_tag="EYO68_06320"
     CDS             15218..16258
                     /locus_tag="EYO68_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIC07455.1"
                     /translation="MLNKFAGYFTQDLAIDLGTANTLVYTKQRGIVIREPSVVAVQKD
                     SRGRIQVLAVGEEAKQMLGRTPGSITAIRPMKDGVISDFDITQEMLRYFIKLTQSKFS
                     FFRFKPRIIVAVPSGITQVERRAVKEAAESAGAREVFLIEEPMAAALGAGLPVTEARG
                     SMIVDIGGGTTEVAVISLAGIVYSDSARIGGDKMDDSITEYIKQKYNLLIGERTAEEI
                     KINIGTAFIKEEPRSHEVRGRDLVTGIPKTLVLGEEEILEALSSVCQQIVKTIRNALE
                     STPPELAADIVDRGIVMAGGGSLLSGMDDLLRQEVGLPIFLADDPLTSVAIGTGKVLE
                     EIDLLATIELAS"
     gene            complement(16303..17709)
                     /locus_tag="EYO68_06325"
     CDS             complement(16303..17709)
                     /locus_tag="EYO68_06325"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:PF03917.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIC07456.1"
                     /translation="MQTQVQNETIENTIELAKLYGILKYLPDGQLTHAPFSLSPYKIS
                     AADLQEMTELTAPFSELMISISQNWDFLEHHLEPIAKIDPFLRMLMDCRTDEITQSKQ
                     LLVQRNDFFLIKDEHKKTGQAGDSPESNFAESALRQVELNTVSASFPFLITQISHLHR
                     YLFKQNQLPEIIPNNPLSPVVDAFAKAVRDYGSPDGVMLLISQPSEGNRFDQLGLEQF
                     LWDEHKISTVRKTLADVFEGGCLRQGHLVLEGKTVALSYYRAGYTPDDYRTIEAVKGR
                     QLIESSSTIQVPDLQMQLAGMKKIQQVLTRPEILSIFTSAETSKHLLKTFAEMHTLDE
                     IIETPGGEMQAAEWVSGNPENYVLKPQREGGGNNYFGSEIPKILKTIRQDEQNAYILM
                     EKILAQTHSAVLVVNGRAERLTSVSEVGRYGICFADNGVLKSNKDAGYLVRTKAENIN
                     EGGVCAGFACLNSLVLEA"
     gene            17739..17812
                     /locus_tag="EYO68_06330"
     tRNA            17739..17812
                     /locus_tag="EYO68_06330"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:17771..17773,aa:Cys,seq:gca)
     gene            17828..17914
                     /locus_tag="EYO68_06335"
     tRNA            17828..17914
                     /locus_tag="EYO68_06335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:17862..17864,aa:Leu,seq:taa)
     gene            18126..19412
                     /locus_tag="EYO68_06340"
     CDS             18126..19412
                     /locus_tag="EYO68_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07457.1"
                     /translation="MKYMLFRLLTFSVLYFFCTSSVFAFGPDRRQDQFGIIPGYLVVP
                     APYVYPGLGKGWMLIGYGGNILETNVDAYLVAISGDAEGYFGSVEEIFVIPKYLYLSG
                     IHLNIKKYGLNMYGSRGMESEKDDFNIFVGDKYILNKLETTLSLMERRIEFSLYSQNQ
                     TGRTIEIRDSKGENPQTIPNAIVFKGQRNGAVLHLDWTDDLKDPREGFRLKTTSDFVA
                     AVDTGSPEYNIFSYGLTFYQPILGNSTWAFHYFRSDAFVKTKGNLNLKSILISSGLTE
                     TQADTCILYPTITGCAAQISKAQNTIKANKNGSAHPLGGQDRLRSYPNGRYQAAHTQF
                     YGTELRWNFNTSKDIVDLIFFSDIMEALQATFFWEQGSVAEEKSELGKINRSSYGTGV
                     RLIGGSGNVYRFEASTGNEGPEILLIFQYPWSGETG"
     gene            complement(19516..20820)
                     /locus_tag="EYO68_06345"
     CDS             complement(19516..20820)
                     /locus_tag="EYO68_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIC07458.1"
                     /translation="MSFSSYFTHKSGGDRIFAVEAPKIKFGRDSLLEIGDDAKVLGMT
                     RVAVFTDQRVGQMEHVAKVVESLKSQGLDAVVYDQVAVEPTDVSFKSGAEFAQDGKFD
                     GFVSVGGGSVMDTCKASNLYSCYPADFLDYVNAPIGKAIPVPGELKPHIACPTTFGTA
                     SECTGIAIFDLLEMESKTGIVSPRIRPNLGVLDPSVLSSLPPLVRAANGFDVFSHACE
                     SITARPYTHRPAPESSQKRPLSQGANPYSDLACLEAIKLIGTHIEQAVNDPVEESYDA
                     LMFAGMLAGIGFGNAGCHLPHGMSYAVAGLVKDYAPEGWPTDHSMVPHGISVIVNSPA
                     VFRKTGPACAERHWKAAAAMGADLTDTKVEDGGDILADQILGMMRETGIPNGLSGVGY
                     SMDDLDALTDRSYAQKRLIDNGPMPISRDELKEMFRDAMSYW"
     gene            complement(20971..23211)
                     /locus_tag="EYO68_06350"
     CDS             complement(20971..23211)
                     /locus_tag="EYO68_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIC07459.1"
                     /translation="MNSNSIQSRIGSATISRTESVVAGELINFTITYTAGYFGIDDSG
                     SIKICTRFATDMGRPQFTAPEQPNYVSIVASNGATLEYRFDPKDNIRPWDKTIYIKIV
                     KGFFREGDQLLVHYGDPIGGCSGMRMQTFCEDTFELKVLVDAFATYQYVEIPDSPVLN
                     IVPGPATKWVMQLPTMRRVNEPFRLQLKAEDRWGNPTDKTDASFKLTSNLPVKGLPQE
                     INQDTQNDGVLLHEGLSVSSPGDIYIELRDSDNALVSRSNPLRIVEDSDLLPFWGELH
                     GQSEETIGTNSIYDYFSFARDKAGVDVIVHQGNDFQITNEFWEKIQNMSKQFLDEGHL
                     VTFPGYEWSGNTGLGGDRNVLFFHEGETIRRSSHALVSDLTDIDTDCNSSDALFQSLK
                     GSETVVFAHVGGRYADIQSHEGNLERSVEIHSAWGTFEWLLEDALRNGYRVGIVSNSD
                     GHKGRPGASYPGASMFGAYGGLTCMLARKLTREMIWESLLRRHHYGTTGNRMFMDVSV
                     LLNKPGSSFREDPQLGETDAENTQVGIMGDILQTEESEVKLEIEIIASAPIEKLEIRN
                     GLKVLETVRPYKKAELGNRIRVIWAGAEYRGRGRETVWDGCATLVGNTFETFTPINMY
                     NLEKTVRQLDPTRLEWNAVTTGGFGGFDCMLKDFQAGSITIKTSLVNCTVPVAEIGFQ
                     DSRIDAGGLDRHLRLVRLPDKNPHYQLSLERSIPLNSKGDNPLYVCLTQEDGHQAWSS
                     PIYLFN"
BASE COUNT         6851 a         4737 c         5254 g         6381 t
ORIGIN      
        1 ttataattaa acaccacgca tcattaagta tgaaaagtct aaatttgact aatcaagcca
       61 gtttgaccac tggcaaagac tttggtggtt tttccttagc atcctgtttg aaagggtgat
      121 ggatctcaaa aaacggctga accaactcga agatatattg aaaaaaaact taatctatat
      181 gaaatccact ttctttaaat atgagtcttc taagtatttt tctgatggat gaacccattc
      241 agattgaacc cattcggcgc ctcccccaca taagagacct cgtcaccgat gtctcctgga
      301 attacgaaat caatcaacac attcgtcctc tcaaaccaaa acccagggag gccgatggaa
      361 cttacaggat gcagcagaag gacatcgagc gcattcagga attccacaaa tgtatcgaat
      421 gcttcctctg ccagaatgtt tgtcatgtca tgcgagacca acaggttaag gaatttgcag
      481 gaccgcgttt cctcattcgt attgcttcac tggctatgca tcctctggat accctcaatc
      541 gactgaagga actcaaagac gtcttcggca ttggctactg caacattacc aaatgctgta
      601 ctgaagtctg tccggaagat atcgcgatca ctgacaacgc aatcattcca ctcaaagaac
      661 gagtcgctgg agccttttac gatcccttgg cgtggctgtg gagaagcctg acttctgtgt
      721 caaaatagtc acgcagaggc ttcaacaaaa tttcatgaaa aagttagaat gaaataattg
      781 ttctctaagt tcctacctct agctacaaaa ccttatgaaa accgctccga attatctgaa
      841 tcccgaaatt ccatcaggcc ttaagcgagt gacaattcca attgtcgaag ctacagcaga
      901 gtcacttgcc ggttacgggc atattgtcag tgatacggat gaggtcgaaa ttgaaatagt
      961 tcgctggcct gcgcaaggtc atcgagaggt cgatccggac agcggtgatg aggggggaac
     1021 aacggaagga ctttttatct gtgaatggaa gggagatatt ctttacggac gcaattctgc
     1081 agtaagcggt cactacattt taggatacgg tactgaacca aagaaggctg atgaacatca
     1141 tacacgggat ccgaaaaccc tcttggtgtg gcatgccaat tatcatccgg atggcggtca
     1201 gtgcttcttt cctgaaacga aaaaaccgtt cgtcgttcct ttggctcttc cgggggacga
     1261 tataagtccc gaagattttg tgtgttttca tttcagtggt cacgagggtc tatacatcca
     1321 tcccaatgtg tggcacgagg gagctttagg aattagtggt gaacagcgtt tttttgataa
     1381 acagggcgct gtgcatgcac gtatatctgt cgattttgcc cgtgaattta aatgtctcct
     1441 ggaagtttcg cttgagcagt ttaatcctgc ttgagaattc agtgactgaa aaaaataatt
     1501 tactttgaag ggaaggattt gaaaatggaa aaatttgcac cttacttgat gtttaacggt
     1561 aactgtgaag aagcagtcaa tttttataaa gaatgtttca acggcgaaat tggtttaatg
     1621 ggaagatacg gagattcacc aatggaggtt ccggaatctc gtaaagataa aatagtgcat
     1681 gtagaattga agttttgggg aggctctatt ttagcctctg acaatatgga cagtacagaa
     1741 agttctaaaa cagatagctc atgtgttcat ctgagtttag gtttcgagga tcctctcaaa
     1801 atggagcgaa cttacgaaaa actcaaacag gacggacagc caacgatgcc actgaaagaa
     1861 cagttttggg gtgataaatt tggaatgtta actgataagt ttggtattaa atggatgttt
     1921 aattgcccaa aaaaagaaag tgcataatcg aataaaaatt caagtaaata tctctacctg
     1981 gtataaaatt catcaaaaat tcttttcagt ttttccatat ttgcttgcaa actctgaaat
     2041 aaacctgaaa catgccggat aagcttccca gggaattcac actatccggt ttgagggaag
     2101 gacgttccaa tgtaactgtg attaccggat cgggaattga cgttgagtcc ggactgccct
     2161 cctttcgcgg tgaaaaagga tattatgaag ataaagagac ggtttatctt gcttctgtgg
     2221 atgcactcaa ttcagaaccg atccgtcaat ggcgctggta cctgaagcga tttgtcagct
     2281 accacgcaac ccctcctgct gcctcccatt tttctctggc tgaacttgaa gctgaaatag
     2341 gtgaaaaatt caacggaatt gttactcaga acatttccgg tctgcaccgc aaggccggca
     2401 gtcagaaagt ttttgaaatc cacggttgca ttcgggaaat gagaaatctt aaaaccggag
     2461 aacgaagacc tttgccgcaa tcttgggtcg aatcacctcc ggaggatgaa gaattatgcg
     2521 gttggcgacc gcatgtctgt tttataggcg agagctatga tgactaccca cttccggaat
     2581 ctgttagtgc atgcagtagc tgtgacattt tacttgtgat tggaaccgca ggtgtaattc
     2641 acactccagt atggctggcg gaacaggcac gactttccgg agccgttgta gtcaacatta
     2701 atccaaatcc cggagagctt gataatgtct cagagttcag cttccgtgga acttcgtctg
     2761 agtattttca ttatgatata tgattgaata tatatttaat catatatgac ggtcacacgc
     2821 aaaaagactt aggtctacaa tttgtcattt taccttagag ataaatctta atgatattag
     2881 cgtaatagct tctgctagat ttctcgcttt gctctaaatg acacggtttt gggtcttttt
     2941 acaaccgtat catatatatt tacgatgtat aaaatttacg ggcgatttag tttagctttt
     3001 taggtcttgc tgaaaaatga tattattcaa taatatcaat aagcaattat tcgattgatg
     3061 atgtcaattg cagagtgttt cctgaaaaag ctctaaaagc ctcgcacttc agttgtttga
     3121 gtccattttc aatcaaggtg acctcacgag aaaattagtt gtgcctctct agattttctc
     3181 ctttgtcatt ccgggcgaag catagcatag acccgggatc caggggtaac acttcaaata
     3241 ttgtactcaa actgaacagt gacctggatc ctggatcaag tccggaatga caaaagaaaa
     3301 actttttcag cgagtcctat ttagaaatag atgaaaaata ttctaattat tttgcagatt
     3361 acatgtgttt gtctgagcac aatatcctgt gcaactaaaa gcaacgactc tgcaaaaggc
     3421 gttttctttt accgtgaatc atttggcgaa tggggctggc atgaagatgg cgatgaggaa
     3481 aaggatggga tatacgaagg tgaaaccaga aaagggaaac cggatggaca aggaacctac
     3541 actcattaca gcgagactaa atcttcagta ttactgctgc cagtcatgat tcctggatgg
     3601 ggtgaaagta tagtatttgc tctaattatc tgggaaatgc tgcatcaggg gcaaccaaaa
     3661 attcaggatg ctaaatatgt cggagcctgg aagagagggg aaaaacacgg tgatggtaca
     3721 tactatttcc ctaatggaga caggtatgta ggagaatgga gaaggggggg aaaacatggt
     3781 gaaggagtat attccttttc tgatggagac aggtataagg gaaactggag ggatggaaga
     3841 aaacatggac aaggtacata tactttttct gatggcgata tatttgctgg agaatggaag
     3901 gatgggaagg tccatggtaa cggaacttac acttatcccg atgggtcaaa gcttatagga
     3961 aaatggaagg atggaaagaa aaatagagag ggtaaactaa ttctttctga ttgataaaaa
     4021 ttaataatca taaaaatgaa ttcaattaac accacctctc ctgctaccga aataattctg
     4081 tgtctgttct aaaacatttg ttaatatgga gaatggaaag aggataatta actaaactcg
     4141 ttgaaacaaa ccaaaatcaa tactgtgcaa aacaaactaa ttaagatcag tatggaacaa
     4201 aaattttctg ttttactttt atttaccctt ttttttactt caatcttttt tgcagttcca
     4261 tttttttcta cttccatttt ggctcaatcc aaagaaaccg gtgtcttata tctttgggaa
     4321 atatcttccg gaaaggtctg gaagagtttt ggtgatgatg aaacacagca aaaatacaaa
     4381 ggtgaaataa aagaggaaaa accagagggt ttgggagtta ccttattttc tgatggaacg
     4441 aaatacatgg gtcagtggca cgacgggatg caacacggac agggaacctt catttttact
     4501 tccggagaaa aaatgactgg agagtggaaa gaaaatgcgg agtggaacat tacaaaatac
     4561 gacattaacg gaaaaataat tgccaaatat gcagatggag ttttgctgat tgacaataaa
     4621 aaagaaggag ttttgttttt tcgtcaggag tttggaagta tggaatggtt tgaggcaggt
     4681 gacgaagaac aggattatat atatgaagga gaaattgaaa acgacaaacc caacggatgg
     4741 ggaacattta cctatccatc aggaagtatc tatgaaggag aattcaaaga aggtaaattt
     4801 catggtcaag gaacctttac ttctgttgaa ggtaaaaaag gtgtgggtga gttcaaagaa
     4861 aataaaccct ggaacgttac tgaattcgac aaggacgata agataatttc cagatatact
     4921 gacggcgttg agataattga aaaaaaattg gtgggagttt tgtttacccg taaagaaaaa
     4981 gggaaatgga tctggtatga aaatggagtt gaagatgaag gtggaaaata tgaaggggaa
     5041 attgaaaacg acagacccaa cggcaaggga actttaattt atcgcaatgg aacaaggtat
     5101 gttggagaat acacggatgg agcttggaac ggtatgggaa ccttctattt tccggatggt
     5161 gaaaaatggg tgggtgagtt cagagaagat gctccatgga acatcaactg gttcgacaga
     5221 gacggaaata tcattgcaaa gtggggtaat ggggttaaac tgaaatgaaa tccggaaaaa
     5281 agggagcggc tctttaatga gttttttccg acttcagtct tcaactatcc aacttaccat
     5341 gaaaaatatt tttcactaat caccacattt ctgaaatcag caatcttgta aaatactgca
     5401 attaatgtta taaaatgagt tatctaaaaa ctcaaaaaaa tatcatgtct caaaatatag
     5461 ttccggtatt ccctgtttat ttcatgaggg ggttgctcaa gcgaattttc aatacaattt
     5521 ttctttgtct gtttctgcca gttttttcgt ttgcagcagg caattttcag aagggttatg
     5581 aggcccatct acggggagac ttctccgctg ctatcaaagc atggggacct cttgctgcgc
     5641 aaggagacgc ggctgcgcag ttcaatattg gaaatttgtt cgacttcggc aaaggcgtaa
     5701 gagaagacaa tgtacaggcg gcttactggt ataacaaatc tgcagaacag gataatgcaa
     5761 atgctcagta tgcacttggg tggatgtacg aaaacggtga aggtgtaaag aaggatctcg
     5821 taaaggccct tctctggtac aaaaaagctg cagaacaaaa acattccggt gcacagacaa
     5881 atcttggctg gatgtacgaa aacggaaaag gtgttgaact ggatcacact caggccgttt
     5941 tttggtataa aaaagccgca aacctgggta gggctgaagg acagttaaat ctcggcatta
     6001 tgtacaaaaa aggccttgga gttgaccttg atcaatctaa ggcagttttc tggtttgaga
     6061 aagcagcttc acaaggatat gcaaatgcgc agttaaatct tggtgttgtc tatagaaacg
     6121 gtgaaggtgt ggaaacagac tacattaagg ctgcttactg gctgaaaaag gcagttaaaa
     6181 cagggcactc tgctgcacaa actaatctcg gctggttgta tgaaaacggg aaaggagtgg
     6241 ctcaggatta tcaacatgct ttctcattgt atcaaaaagc tgccgaaaaa gggcaggctg
     6301 aaggacagtt gaatctgggt gttatgtatg caaatggtaa agggataaaa gttaattaca
     6361 aaaaggccgt ttactggtac agaagagctg cggatcagga acaggcagaa gcacaattga
     6421 atcttggtat tatgtataaa tacggaagag gtgtagagga aaactacaca caggcggttt
     6481 attggtttaa aaaacctgct gaaaaaggat atgccgaagc ccagaccaat cttggcctga
     6541 tgtacgaata tgggaaaggt gtgaaacagg attatacaga ggctatctta tggtatgaaa
     6601 aagctgcaga acaggacgac gtaacatcac agttgagcct cgcacttttt tacaaattcg
     6661 ggaagggtgt ccgcaaggat tatacacaag tgttttactg gtacaaaaaa gctgctgagt
     6721 tgggagacac aggcgcacag ttaaatctgg gatttatgtt tcaatacggc aagggcgtag
     6781 aacaggatta tgaagaagca gttacatggt acaagaaatc ggctgaacgg ggacatccag
     6841 aagcacaggc cagtcttgcc tggatgtatg aaaacggcaa aggaattgaa gctgatctta
     6901 aagaggcaat ttactggtac aaaaaatccg ctgagcaggg taacacaaaa gcacagttga
     6961 aactcggcat catctacaaa aacggcaatg gtactgtgga ggattacacc cagtcttttt
     7021 actggtacaa aaaagtagcc cagcagggtg acgcacacgc gcagttaaac gtaggaataa
     7081 tgtatgagta cggaaaaggt acaacacagg attataccaa tgcagtacat tggtacaaaa
     7141 gtgcggctgt gcagggactc tctgaggcac agactagact gggttggatg cacgaaaacg
     7201 gtaaaggagt ggaacaagac tactctcagg cgattagcct gtataaaaat gctgctaaaa
     7261 atggagaaac aaatgcacag ctgaaactcg gtattatcta ccaaaacgga aaagttgccg
     7321 cggaggatta ttctcagtct tttttctggt acaaaaaagt tgctgagaag ggtgatgcaa
     7381 atgcacagtt gaatcttggt cagatgtatg agtatggaaa agggactgat gaaaactata
     7441 cccaggcagt atactggtac aaaaaagctg ccgagcaggg agatactgaa gcacagctca
     7501 atctagctgt tatgtataaa tacggtactg gagtaaatga agattcttcc caggctgtat
     7561 tctggtataa aaaagctgcc gagcagggga atacaaaagc acagctcaat ctagctgtta
     7621 tgtatgcctc aggtgaaggg atactggaaa attttgtttt agcctataaa tgggctaacc
     7681 tggctgcagc acggggcaac gaaaatgcaa gagaaatgaa aaacgaggtt aaaaaacgta
     7741 tgacccgtac tcaaattgct gaggcacagc aactctcgct ggaatggcag caaaaccaat
     7801 ccggtgcaga aaactgatac ggaatctctt tttataaatt aatttatggg tcgttctaca
     7861 gacctatcca cttaacaacc ttactgaagc tcttttcata aatgtacacg cttcattctt
     7921 tttaatttcg gaaacttctt ttttagtctg taaatcattc cgcctgcctc tctctttgtt
     7981 tcaaaaaatc ctatccgtat ccgatacgcc agcttacctg atgaagcaga tttcagcttt
     8041 tgtgaaatat aactgaagtc gatttctgta tttaattcag caagatcttt cagcgcctta
     8101 ttctgcacat cgtaaagagg aagttcaata acccacggct tgttcaaagg ctgtcttaga
     8161 tcaatcaccg gacgtgaaag ttcttgtggg cccggctgga cgggcatgaa gttttcagaa
     8221 aacaattcct catccttaaa tttttcaaat atttttttgc ctgtagtgag ggcatcttca
     8281 agatttttaa aaaatcccac tcttactcga taccagaatt tcccatcaaa ttctgcatct
     8341 gtccgatgaa gatatgcata gtgtccggca tgcagaagtc gactcatcaa agaaactgcc
     8401 agttgaaact gtccaccttc aattgaaacc agttgaaccg aaaacttacc ccgttttttg
     8461 cggtaaagaa tgtcttttat tgccgccagt tcagagcgga actgaactcg tatagaattg
     8521 ttaatcctga caagatgcca gtccctctgg gaaagtggag taatggtgta gtcaattttg
     8581 aagcggttct gcactagaat agtgtgccag ttctctgctg atacaccggg cttgttgata
     8641 ggaaaaacat tccagaatgg ctttgaagtc tctactaaat gcgaatactc tgcgtcttct
     8701 gaaatgtcag ctgttcctac tgctcctggc tttggttcag gacccgcttc ttcgctgact
     8761 ggttcaatcg tttcgctatc tgcctcaact tctggagagt caatttccag tgagccaact
     8821 atattttcct gatttacagc catttgtaca tagatcagta ctgaaactat tccacccatt
     8881 aaaaatgtgg acaaccagag cagcaaaagt ctgataaaac gttttctatt cacttgttca
     8941 ccttagttcc tgattttatg acacgccgta aggcaatttt gattcgcatt tttggtttgt
     9001 agtagtatat aatacagggg ctgaaagttc agatccatgc aatgtaatac aataattttg
     9061 aaagccggat taaaattgat aaaaaattaa ctgttcaatg aatattatta acgacacact
     9121 ttttttggaa gacatatctg ttgaaacact gatgcaggaa tatggatcac cactctatgt
     9181 ttacgaggag tcagtcttgc gttcacagtt ccgtcaattg tcttccgggt ttcctgaagg
     9241 actgctggaa atacattatt ccatgaaagc aaattctaat ccatcactat tgaaaattct
     9301 ccgagaagaa ggttcgtcaa ttgacgcagt atcagaattc gaggtgcgtt tggctctgga
     9361 atcaggattt gagccgcagc aaattatttt taccggtaat aacaactccc ttgcagaaat
     9421 tgaatattgt gtggagcagg aggtctcagt caatctgggt tcactcgttt tgctggaaaa
     9481 atttggtcaa cgatttccac agacaagcgt atctgtgcgg atcaaccctg gaattggtgc
     9541 tggtcatcac gcacactgta tcaccggtgg tccggattca aaatttggaa tttatcacga
     9601 tcaaatcgat gctgcacttg cactggcaac aaaatataaa ctaacattga acggtgttca
     9661 ttctcacatc ggtactggaa tttattctgc agaaccaatg ctggaggcca tggaaatgat
     9721 tttagctgtg gctgaaaaat ttccggattt ggagttcatt gattttggcg gcggttttgg
     9781 aattccatac agaccggaac agcaggcttt ggatatgaaa gatttaagtc aaaaaatgac
     9841 caggcgcttt acggaattca ggcagtgtta cggcagagaa atatcaatga aaattgaacc
     9901 agggaaattg ctggtaggac ctgctggaat tttgttgaca actgtcacaa acatcacgga
     9961 tactccaaaa caccgctttg taggagtaga ttccggattc aatcacctgc tgcgtccgac
    10021 gttgtacgga tcctatcaca aaatccttaa tgccagtcga tattcaggtg atgaagagga
    10081 tgttgttgtc gttggatata tttgcgaaag cggtgatatt tttagtagat ccggagatga
    10141 aattgagcgc aagttgcctt ctccagttat cggtgaacgc ctcgcttttt tagatgctgg
    10201 tgcatatgga ttttcgatga gttcacagta taattcccgt ccgcgtccag cggaagtact
    10261 ggtgcatcaa gggcagagcc gtctcattcg gcgggctgaa acttttgaag accttgcaca
    10321 gttttttgtg gattttaatt agcaatttgt agaaatgaac aagaaaaaac ttgggaaaca
    10381 aaaaggccgg ctggtcgatt cagaagctct gctggaagtg cagaaactgc tgtcggacat
    10441 gccgctccgt aaggacatgc tgattgaagc tttgcattta attcaggacg aatttcatta
    10501 tttatctgca aagcatctag tggcacttgc tcatgaaatg aaacttgctc atgcagaagt
    10561 ctttgaggtg gccacgtttt atcatcattt tgacgtgatc aaagaaaacg atgtgcctcc
    10621 gccacctttg acggttagga tttgcgattc actgacctgt gaaatgtccg gagcaaaaga
    10681 actcacggaa tcactaaaaa atgttttagg tgaaggtgtc cggattcagc ctgtgccttg
    10741 tgtgggacgc tgtcaaaatg cgcctgtggc tgtggtgggt aaaaacccgc ttgatctggc
    10801 aacaattgaa tctgtacaga atgcagtaaa taaaaaagaa gttcgtccgg aaatccctga
    10861 atatgtgggt ttttcgcaat atcgggacac cggtggatat cagctttttc aggaatgcaa
    10921 ttcaggaaag cgaactgccg aatctgtgat tgatgaactg aaaaattctg gtctgcgtgg
    10981 tttaggcgga gcaggttttc ctgcaggaag taaatgggaa attgtcaaaa gattttcagg
    11041 gcctcgattg ctggcagtca acattgatga aggagaaccg ggaacattca aagaccgctt
    11101 ttatcttgaa actgatccgc accgtttcct ggaaggatct ttgattgcag cctgggccgt
    11161 tgggattgat gaaatttata tttatctccg agacgaatac gctgcggcaa atgaaattct
    11221 gctccgggaa attaaagaac tgcaatctgc ttttccggat atacttccac aaatctattt
    11281 acgccgtgga gccggtgcat atatttgcgg tgaagagtcg gcgatgatag aatcgattga
    11341 gggcaagcgc gggatacctc gtttgcgtcc gccgtatgtt gctgaagttg gtttgtttga
    11401 ccgtccaaca ctggaacata acatggaaac tctgcactgg attcgtgata ttgtagaaaa
    11461 aggcgcaaaa tcattttctt ctcacggcag aagaggacgc aaaggtctgc gcgcattttc
    11521 ggtcagcggc cgtgtgcaaa aaccgggagt gcatcttgct ccagcaggaa tttcgatgct
    11581 cgaattgatt gacgaatatt gcggcggaat gctgcccgga cacagctttt atggttattt
    11641 tcccggaggt gcttccggag gaatgcttcc agcttcactt ggagacttgc cgctggattt
    11701 cgatacactt caggaacatg ggacttttat tggttctgct gcagtgattg ttttttctga
    11761 tcaggatagt gcacgtaaac tggcacttaa cgcaatgaga ttctttgaac acgaatcctg
    11821 tggacaatgc actccctgca gggttggaac agcaaaagcc attccactga tggaacaaaa
    11881 aacctggaat aaatcccttt tagaagaact ctcgcagaca atggaagatg cttcaatttg
    11941 cggtttgggt caagccgcac cgaatccgtt acgctgtgta atgaaatact ttccgcagga
    12001 ggtgtaaaat ggagacaatt tcatttgaac ttaacggaca aacgcttgag gctttggtcg
    12061 gcgaaacgat tttgcaggcg gcggatcgtg caggagtcgc agacattcct cgactgtgtt
    12121 acaaagaggg ttaccgtacg gacggcaatt gccgctcctg tgtggtggaa attgaaggtg
    12181 agcgcgtact tgcgccgtcc tgctgtcgtt acccgcagca gggtatgaaa gtcaacagca
    12241 agagcgaacg tgcagtccat tcacaaaaaa tggtgctgga actgttgctg gctgatatgc
    12301 ctgaatccgg aaaatcgcct tacacaccaa attcggaact tgatgaatgg gtggaaaaat
    12361 taggtctaag caagccccga ttctcaaggc gggtccaacc ggcttttgat aactcacatc
    12421 ctgcgatgtc agttaatttg gatacctgca tccagtgtac ccgctgtgtg cgtgcctgcc
    12481 gcgaagtgca ggtaaatgat gtgatcggaa tggcctatag gggatcgcac gcagaaattg
    12541 ttttcgactt cggcgatcca atgggtgaca gtacttgtgt cgcctgcgga gaatgcgtgc
    12601 aggcctgtcc gactggtgcg ttgatgccgg caaatgatgt aggcttggcg gccccagaca
    12661 aaaaggtcga atccgtttgc ccttattgcg gagttggttg tttgctgact tatcatgtca
    12721 aggaagggaa actattacgg gtcgatggac gcgaaggtcc ggcaaatcat gaaaggttgt
    12781 gcgtcaaggg acgttacggt ttcgattacg tgcatcatcc ggatcgtctt acaaagccgt
    12841 taatacgtag gaaagatgct ccaaaaacaa ttgaactgct ccagcctaag gactggcaaa
    12901 aagttttcag ggaagcaact tgggaagagg cactcgattt tgctgccgat ggtttcagca
    12961 aaatccggga tgaaaacggt ccggatgcat tggccgcttt tggctgtgcc aagggcagta
    13021 acgaagaagc atatttgctg caaaaattag ttcgtactgg ttttggaacc aataacattg
    13081 atcactgtac ccgtctctgt catgcctcat cggtaagtgc tttgctggag ggcattggat
    13141 ccggagcagt ttcaaatcca ttcacagatg tgacggatgc tgaagttatt tttctgattg
    13201 gagcaaatcc catcgggaat catccggtgg ccgccacgtt tatgaaaaac gcagtcaaag
    13261 ctggtaaaaa gttgattttg ctggacccac gccgtactgc gttatcacgc catgcaactt
    13321 acaacctcca gtttcgtccg gataccgatg tggcactgct caattccctg atgcatgtca
    13381 tcatcaaaga aaaattatgc gacgaaaaat acatcacaga acacactact gattttgaaa
    13441 aattgaaagc caatgtcgcg gattacagtc ctgaaaaggt agctccaatt tgtggaattc
    13501 cggaagaaac tttacgtgaa gttgcccgga cttatgctac tgcggaatca gctatcatct
    13561 tgtggggcat gggaatttcg cagcacattc atggtacaga caattcacga tgtctaattg
    13621 ctttgtcgct gatgacaggt cagatcggac gtcccggaac cggcttgcat ccactgcgag
    13681 gacaaaataa tgtgcaggga acatctgata tgggcttgat tccgatgttc tttccggatt
    13741 acaaatcagt tacagatccg gaaaacaaaa aatggtttga gaatttttgg ggaacctccc
    13801 tcaatccaaa aactggttta acggttgtgg aaattgttga tgcagtgcat tccggaggag
    13861 tccgcggcat gtacgttcaa ggtgaaaatc cggcgatgtc tgatccaaac cttaatcacg
    13921 ctcgtggtgc attgtccatg ctggagcact tggtggtgca ggatattttt ctgactgaaa
    13981 cagcaggttt tgcggatgta atttttcctg cttccgcttt tccggaaaag agcggcacct
    14041 tcacaaattc caaccgccaa gttcaacttg gacgacaagc catcgatcct cccgggaacg
    14101 cacgtcaaga ctggtggatc atacaggaag tggcgcgacg acttggtgtt gattggagtt
    14161 atgatggtcc tgaagaaata ttttctgaaa tcaggaaagc aactcccagc atggccggta
    14221 ttacctggaa acgcttacag aaagaacatt ctgtgacata tccctgcaac aaagaaggag
    14281 atcccggaga agcaatcgta tttagaaaag gtttccccac agcaaataaa aaagcccgct
    14341 tcgttccggc aaaatacact catgcagacg aaatgcccga tgaagaattt ccgtttgtgt
    14401 tcatcacttc acgccaactc gaacactggc acacaggcag catgacccgg cgtgcaactg
    14461 tcttgaatgc cattgaacct tatccggtgg tccatgttca ccctgatgac ctgcaaaagc
    14521 tgggagtcga acctggagaa gccgtaacta ttgaatcacg tcgaggaaaa atatcaacgt
    14581 attcccggca ggatcccggt gttcaacagg gaaatctttt tatgtccttc ggttacaatg
    14641 aggctgccgc caatttgatt actacttctg tgctggatcc ttttggaaaa attccggaag
    14701 taaagttctg tgcagtacga atgtctgcag gaggaaatcc tgaaatgcgg atcggctgac
    14761 ggcttacatt attttgatca cgtccggagc aacagagaaa agatcacata agtcgagaaa
    14821 gtcctttttc ctcttgtttt ctagtctttc cttgattaaa atcaatgata ctgcttttgg
    14881 aagaatttcg tttactgctg ctttactcaa cttctgcaac catatacagg actgaaaata
    14941 ttagaagtta cggcataata aattggttaa gaacttaaaa gtagtttgct gaacatacat
    15001 gcaaaatact tttaatgata tacaaaataa atcaagtctc tttgagtttt ggcattactt
    15061 ctgtattacc ttctgaagcg tataaaaaac gtaaaaatac tgtttttgca cacaggattt
    15121 tcgcatgtca ataaactaaa gaatttcaat atttttataa ttgccaatgc atggactgtg
    15181 agttgactat gctggaaaac aaaactagga atattcgatg ctaaataaat ttgccggtta
    15241 ttttacacag gatttagcga ttgatttagg aacagctaat accctggttt atactaaaca
    15301 acgtgggatt gtgattcgtg aaccatcagt ggtggctgta caaaaagaca gccgtggaag
    15361 gattcaggta cttgctgttg gggaggaagc caaacaaatg ctgggtcgta ctccaggttc
    15421 aattacagct atccggccaa tgaaggacgg cgttatttca gatttcgata ttacccagga
    15481 aatgctgcgt tactttatca agctgacgca aagcaaattt tccttctttc gttttaagcc
    15541 gcgtattata gttgccgttc cttcaggaat tactcaagtt gaacgtcgtg ctgtcaaaga
    15601 agctgctgaa tctgcaggag caagagaagt ttttttgatt gaagaaccaa tggccgctgc
    15661 actaggagct ggattgccgg tgacagaagc cagaggcagc atgatcgttg atattggagg
    15721 aggaactaca gaggttgctg tgatttcctt agcaggcatt gtttatagcg attctgctcg
    15781 gattggtggt gataaaatgg acgacagcat taccgaatac attaagcaaa aatataatct
    15841 gctcattggt gagcggactg cagaagaaat taaaatcaac atagggactg cttttattaa
    15901 agaagaaccg agatcacatg aagtacgcgg ccgagacttg gtaaccggta tacctaaaac
    15961 actggttctt ggtgaagaag aaattctgga agctctcagt agtgtttgtc aacaaatagt
    16021 taagacaatc cgaaatgccc tggaatctac tcctccggaa ctggcggctg acatagttga
    16081 ccgcggaatc gtaatggcag gtggaggttc attactttca ggcatggatg atttgcttcg
    16141 acaggaagta ggcttgccga tttttcttgc agatgacccc cttaccagtg ttgctattgg
    16201 aacaggtaaa gtgcttgaag aaattgattt gcttgctaca attgaactcg catcttgaga
    16261 agttttaata cttagatgta atcctgaaaa ttaatgatag ccttaagctt ccagaacaag
    16321 tgaattcaag caggcaaagc ccgcacacac tcctccctca ttgatgtttt cggcctttgt
    16381 acggaccagg tatcccgcat ccttgttgct cttcagcact ccgttatcag caaaacaaat
    16441 tccatatctt ccaacctcac tcacactggt taatctttct gcacgtccgt tcaccacaag
    16501 tactgcagag tgagtttgtg caagaatttt ttccatcaaa atataagcat tttgttcatc
    16561 ctgcctgata gttttgagaa tttttggaat ttcgctgcca aaataattat tcccgccacc
    16621 ttcccgttga ggtttgagaa cataattttc aggattgccg gagacccatt cggctgcctg
    16681 catttcacct ccaggtgtct caataatttc gtccagtgtg tgcatttctg caaatgtttt
    16741 taataaatgc tttgacgttt cagcagaggt gaaaatagat aaaatttccg gcctggtcaa
    16801 gacctgctga atttttttca ttccagcaag ctgcatctgt aaatccggta cctgaattgt
    16861 tgatgatgac tcaatcagct ggcggccttt tactgcctca attgttcgat aatcatcagg
    16921 agtgtagccg gcacggtaat agctgagggc aactgttttt ccttccagaa ctaggtgtcc
    16981 ctgacgcagg catccgcctt cgaatacatc ggctaaagtt ttccggacag tactaatttt
    17041 atgttcatcc cataaaaact gttccagccc caactgatcg aaacggttgc cctctgaagg
    17101 ttgggaaatc agcagcatca ccccgtcagg ggaaccgtag tctcgcactg cctttgcaaa
    17161 ggcatcaact acaggactca gcggattgtt tggaataatt tctggtaatt gattttgctt
    17221 gaataaatag cggtgtaaat gagatatttg agttattaaa aacggaaaac tggcagagac
    17281 cgtgttcagt tcaacttgac gaagtgctga ctcagcaaaa tttgattcag gggaatcacc
    17341 tgcctgccct gtttttttgt gctcatcttt gatcaaaaag aaatcatttc gctgcacaag
    17401 aagctgtttg ctttgagtaa tttcatccgt tctgcaatcc atcagcattc gtaaaaatgg
    17461 gtctattttg gcgataggtt ccaaatgatg ctcaagaaaa tcccagttct gagatatgct
    17521 aatcatcaac tcactgaatg gtgcagtcag ttctgtcatt tcctgcaggt ctgctgcaga
    17581 aattttataa ggactaaggg aaaaaggggc atgagtgagc tgaccatcag gcaaatattt
    17641 cagaattcca taaagctttg ccagctctat cgtattttca atcgtctcgt tttgaacttg
    17701 tgtttgcatt caaatttcgg taattttaga tgaccagagg cgacgtggcc gagtggttag
    17761 gcagaggtct gcaaaacctt gtacagcggt tcaaatccgc tcgtcgcctc cagttccttt
    17821 tttccctgcc tgggtggcgg aactggtaga cgcaagggac ttaaaatccc tcggtggtaa
    17881 caccgtgccg gttcgagtcc ggccctaggc accaaataat atacgactta aaacccaaaa
    17941 aaccgtttgc tgataattta gtgaacggtt tttttgtctt tctcctgcgc agatccaaat
    18001 caaaatttaa ctgcgttctc actcttttgc aggaaactgc tccaaagatg tttatctttt
    18061 tcatgcaaat gataatattc catacgtcaa ttatacttta aaattaataa taccaacagc
    18121 ctccaatgaa atacatgctt tttcgtctcc tgactttttc agttttatat ttcttttgta
    18181 cttcgtctgt cttcgctttt ggtccagaca ggcgtcagga tcagtttgga ataatcccgg
    18241 gatatctggt agtacctgca ccttatgttt atccaggatt gggtaaaggc tggatgctaa
    18301 tcggttatgg cggaaacata ttagagacca atgtggacgc atatctggtt gcaatcagtg
    18361 gagacgctga aggttacttc ggttctgtag aagaaatatt tgtcatacca aaatatcttt
    18421 acctctcagg aatacatctt aatatcaaaa aatacggttt aaacatgtac ggcagtcgag
    18481 ggatggaaag tgaaaaagat gattttaata tcttcgtggg tgacaaatac attctcaaca
    18541 aactggaaac aactttgagc ttaatggaac gtagaataga attctcatta tactcgcaaa
    18601 atcaaactgg acggacaata gagatcaggg attcaaaagg agaaaatccg caaacaatac
    18661 caaacgccat tgttttcaaa ggccaaagaa atggagcagt tctgcatctg gactggactg
    18721 atgacttgaa agatccccga gaaggttttc gacttaaaac tacttcagac ttcgtagcag
    18781 cagttgatac aggatctccg gaatacaaca ttttttcata cggattaact ttctaccaac
    18841 caatattagg aaacagcacc tgggcatttc attattttcg ttctgacgcc tttgtaaaaa
    18901 caaaaggtaa cttgaacttg aaatctattt tgattagcag tggattgact gaaacacagg
    18961 ccgacacatg cattttgtac ccgaccataa ccggatgtgc cgcacagatt tcaaaagcac
    19021 aaaacacaat caaggccaac aaaaatggat ctgcccatcc tttgggcggc caggacagac
    19081 ttcgttctta cccaaatgga aggtatcagg ctgcacatac acagttttac ggtactgaat
    19141 tacgctggaa cttcaacacc agcaaagata ttgttgattt gatctttttt agtgacatta
    19201 tggaagcact gcaggccact tttttctggg aacagggaag cgttgctgaa gaaaaatcag
    19261 agttgggaaa aatcaaccgt tcttcatacg gaacaggagt tcgcttaatc ggaggttcag
    19321 gcaatgttta ccgttttgaa gcttctaccg gcaatgaggg tccagaaatt ctgctcatct
    19381 tccagtatcc atggagcggt gaaaccggct gattgttgac caccttctca tcgttgtttt
    19441 gctgacagac catgaatata aaaatactct gcaaaactgt ataatttatt cactgcatgt
    19501 accatagaaa aagaattacc agtaactcat tgcatcacgg aacatttctt tcaattcgtc
    19561 acgggatatt ggcatgggac cattgtcaat gagccgtttc tgtgcgtaag agcggtcggt
    19621 gagtgcatcg agatcgtcca ttgagtagcc gactccggaa agcccatttg gaattccggt
    19681 ttcccgcatc atgccaagaa tttgatctgc gaggatgtca ccgccgtctt cgacctttgt
    19741 atcagtgaga tctgcaccca tggcagcagc ggctttccaa tgccgctccg cacaggctgg
    19801 gccggttttg cgaaatactg ccggagaatt gacaatcacg gaaataccgt gaggaaccat
    19861 ggaatgatca gtgggccagc cttcaggagc ataatcttta acaagtccgg caaccgcata
    19921 tgacattccg tgtggaagat ggcagccggc gttgccaaaa ccaattcccg ccaacattcc
    19981 tgcaaacatc agcgcatcat agctttcttc aaccgggtca tttacagcct gctcaatatg
    20041 tgtgccgata agcttgatcg cttccaggca agctagatca ctatacggat ttgccccttg
    20101 acttagcgga cgtttttgcg aagattccgg agcaggtctg tgtgtgtatg gtcgtgctgt
    20161 gatggactcg caggcatggc taaaaacatc aaaaccgttt gcagcccgaa cgagtggcgg
    20221 caatgaagac aatacgcttg gatccaggac accaagattt ggacgaattc tggggctgac
    20281 aattccggtt ttagattcca tttccagcaa atcgaaaata gcgattcccg tacattcgct
    20341 ggctgtgcca aaagtcgtgg gacatgctat gtgcggtttg agttcccccg gaacaggaat
    20401 cgcctttcca attggtgcat tcacataatc aagaaaatct gcaggatagc atgaataaag
    20461 attagaggcc ttacatgtgt ccattactga tcctccacct actgatacaa aaccatcaaa
    20521 cttaccgtct tgggcgaatt ctgctcccga cttgaacgag acatctgttg gttcaactgc
    20581 aacctgatcg taaaccactg cgtccaaacc ttgtgatttt agtgattcta caactttggc
    20641 aacatgttcc atctgtccga cacgctggtc agtgaaaacg gccactcggg tcataccaag
    20701 tactttggca tcgtctccga tttccagaag agagtcgcgt ccgaatttaa tcttgggggc
    20761 ttccacagca aatatacggt ctccaccaga tttgtgcgta aagtagcttg aaaaactcat
    20821 caattccttt ctaaataaat tttgtattgg aataaaattc cataatcaaa ttccggtagc
    20881 cgaattacga ttttcaacaa tagcatgatc aagattggac cgaaaccttg aattggcata
    20941 atattacgaa atttttttaa ttaaagtgaa ctagttaaac aaataaattg gacttgacca
    21001 ggcctgatgg ccgtcctctt gtgtgagaca aacatacaac ggattgtctc ctttggaatt
    21061 caaaggaatg cttcgttcca aactcagttg ataatgcggg tttttgtcag gaagacgaac
    21121 aaggcgtaga tggcggtcca agcctccggc atcaatacgt gaatcttgaa aaccaatttc
    21181 ggcaacaggc acagtacaat tcaccagact ggttttaatg gttatcgatc ctgcctgaaa
    21241 atctttcaac atacagtcaa aaccaccaaa tccaccagta gttactgcat tccattccaa
    21301 gcgagtcgga tcgagttggc gtacagtttt ctccaggtta tacatattaa ttggtgtgaa
    21361 tgtttcaaaa gtgttaccaa ctaacgtagc acatccgtcc caaacagttt cacgaccacg
    21421 tccacggtat tccgcacctg cccagatcac ccgaatacga ttaccaagtt ctgccttttt
    21481 gtatggccgt acagtttcaa gcaccttcag accgttacga atttctaatt tttcaatcgg
    21541 agccgatgca attatttcaa tctcaagttt cacttcgctc tcctcggttt gaagaatatc
    21601 ccccattatt ccaacttgag tattttcagc gtcagtttct cccaactggg gatcttctct
    21661 gaaactgcta ccgggtttgt tgagtaaaac actcacatcc atgaacatgc gatttccagt
    21721 tgtaccataa tgatggcgtc gcaaaaggga ttcccaaatc atttcacggg tcaacttgcg
    21781 agctagcata caggtcagtc caccataggc gccgaacatt gacgcgcctg gataactggc
    21841 tccgggacgc cccttgtgtc catcactgtt actgactatc cccactcggt atccattacg
    21901 cagggcatcc tccaacaacc actcaaatgt tccccatgca gaatgtattt ccacagaacg
    21961 ctccaagttg ccttcatgtg actgtatgtc tgcataacgg ccaccaacat gcgcaaacac
    22021 aactgtttca gatcctttga gtgactgaaa taaagcatcc gaactgttgc aatctgtatc
    22081 tatgtctgtc aagtctgaaa ccagcgcatg agaacttctc cggatagttt ccccctcgtg
    22141 aaaaaagaga acattacgat ctcctcccaa tccggtgttc cctgaccact cataacctgg
    22201 aaaggttacc aaatgtccct catcgaggaa ctgttttgac atattctgta ttttttccca
    22261 aaattcattt gtaatctgaa aatcgttacc ttgatggaca ataacatcta caccagcctt
    22321 atcacgagca aatgagaaat agtcatagat agaattggta ccaattgttt cttcggattg
    22381 accatgtaat tccccccaaa aaggcaagag atcagagtct tcaacaattc taagtggatt
    22441 cgatcgacta acaagagcat tatcagaatc acgtagttcg atataaatgt ctcccggaga
    22501 agacaccgaa agaccttcat gcaacagaac accatcattc tgagtatcct ggtttatctc
    22561 ctgtggtaag cccttgaccg gcagatttga agttagttta aaactggcat cagttttgtc
    22621 agttggattt ccccagcggt cttcagcctt cagttgtaaa cgaaacggtt cgtttactcg
    22681 tcgcattgtc ggaagctgca taacccactt tgttgccggt cccggaacta tgttcagaac
    22741 cggagaatcc ggtatttcaa cgtactggta tgttgcaaaa gcatcaacca aaacttttaa
    22801 ttcaaatgta tcttcgcaaa atgtttgcat gcgcataccg gaacaaccac cgattggatc
    22861 accataatgt acaagaagtt ggtctccctc acgaaaaaaa cccttgacga tcttgatgta
    22921 aatcgttttg tcccatggtc gaatgttgtc tttcgggtca aaacgatact caagagtggc
    22981 tccattgctg gcaacgatac tgacgtaatt cggctgctct ggagcagtga actgaggccg
    23041 tcccatatct gtagcaaaac gggtacatat tttgatgctg ccggaatcgt caattccaaa
    23101 ataccccgca gtgtatgtga tagtaaaatt gattaactca cccgcaacca ctgattcagt
    23161 acgggaaata gtggccgaac ctatacggga ttgaattgaa ttagaattca ttttgtagtt
    23221 tat
//
LOCUS       DTVD01000179            1640 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_34963_length_1640_cov_414.054890, whole genome
            shotgun sequence.
ACCESSION   DTVD01000179 DTVD01000000
VERSION     DTVD01000179.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34963_length_1640_cov_414.054890"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="EYO68_06355"
     CDS             complement(<1..732)
                     /locus_tag="EYO68_06355"
                     /inference="COORDINATES: protein motif:HMM:PF01979.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIC07460.1"
                     /translation="MAFIELPTADLTASTFKSKANKWVETPGLVDLQVNGFAGVDFNS
                     PGLTSDSLQLSLEAMLATGVTACLPTIITGSETHLHTCFSALEKARNSSRLAKTMVAG
                     YHLEGPFLSKLPGYSGCHPVEAMCAADPEMFLRLQQAAGGNIRLVTLAPEVEGAIAFI
                     EKLVQDRIIVSLGHTAADNETIQQAVDAGARLSTHLGNGTCPELHKNNNPIIAQLSED
                     RLSASFIADGYHLSPDVLKAYLRAKC"
     gene            complement(857..1639)
                     /gene="nagB"
                     /locus_tag="EYO68_06360"
     CDS             complement(857..1639)
                     /gene="nagB"
                     /locus_tag="EYO68_06360"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIC07461.1"
                     /translation="MEILIRDSLEAGAEVAASVVKKIINSKENPVLGLATGGTPLRMY
                     HELIRMKESGELSFQKCTTFNLDEYAGLPREDERSYYYYMMNNLFDHLDINRERVHLP
                     DGNAKDLREACRKYEQQIKNAGGIDMQVLGIGANGHIGFNEPTGSFASRTWVKILSEQ
                     TIQDNSIYFDKLEEVPRHVVTMGIATIMESRHCLLLANGAKKADAIRKMIEGPISASC
                     PASILQMHPRVTVVLDEESAYLLTFKDHYKWIEKNKLDWQRY"
BASE COUNT          423 a          378 c          362 g          477 t
ORIGIN      
        1 acattttgct cttagatagg ctttcaacac atccggagaa agatgatagc cgtccgcgat
       61 aaaactggcg cttagtctgt cctcactcaa ttgtgctatg atcggattat tgtttttgtg
      121 cagctccgga caggtgccgt ttcccaggtg tgttgaaagt cttgcacctg catctacagc
      181 ttgttggatg gtttcattgt cagcggctgt gtgtcctaag gaaacaatga ttcgatcctg
      241 aactaatttt tcaatgaaag ctatagcacc ttcaacttca ggagccaacg tcaccagccg
      301 gatattacct ccggcagctt gttgcagacg caaaaacatt tccggatcag ccgcacacat
      361 agcctcaacc gggtgacaac cggaatatcc gggaagtttt gatagaaagg gcccttccag
      421 atgatagccg gctaccatgg tttttgctaa tcgagaggag tttcttgctt tttccaaagc
      481 cgaaaagcat gtatgcaaat gtgtctcaga tccagtaatg attgtgggca gacaggccgt
      541 cactcccgtg gccagcattg cttcaagaga cagttgaaga gaatctgaag tgaggccggg
      601 tgagttgaaa tccacccctg caaaaccatt tacctgtaaa tcaaccagtc ctggagtttc
      661 cacccatttg tttgctttac ttttaaacgt agaggcagtt aagtctgcag taggtaattc
      721 gataaacgcc atgtatttct tttgccttag ttaaaaaccc acaaataatc atcctaaaca
      781 gcggtagtcc cgtgtctccg taccggggag gtaaagaatt atttgagggt tagtattttg
      841 ttgtatgctt gttttattag taacgttgcc agtccagttt gtttttttca atccacttgt
      901 aatgatcttt gaatgtcaat aaataggcgg actcttcgtc aagaactaca gttacacggg
      961 gatgcatctg tagaatcgag gctggacaac tggcagaaat tggaccctca atcatcttcc
     1021 ggatcgcatc tgccttcttt gcaccattgg ccagcaacag gcaatgacgg gattccatga
     1081 tggtggcaat gcccatcgtc accacatggc gcggaacttc ctccagtttg tcaaagtaga
     1141 ttgaattatc ctgtatggtt tgctcactga gaatttttac ccacgtgcgt gaggcaaaag
     1201 agccggtagg ttcgttaaaa ccgatatggc cgttggcacc tataccgaga acctgcatat
     1261 caattccgcc cgcgtttttg atttgctgtt catatttgcg gcaggcctcc cgcaggtctt
     1321 ttgcgttacc atccggaagg tgtacccgct cccggttaat atccagatga tcgaataaat
     1381 tgttcatcat gtaataataa taggagcgtt catcttcacg gggcaatccc gcatattcat
     1441 ctagattgaa agttgtacat ttttggaaac ttaattctcc ggactctttc atccggatta
     1501 attcatgata cattcggagc ggtgttcctc cggtagccaa acccaaaaca ggattttctt
     1561 ttgaattaat aatttttttg acaacacttg cggccacctc agcaccggct tccagggaat
     1621 cacgaataag tatttccata
//
LOCUS       DTVD01000180            6676 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3511_length_6676_cov_585.999547, whole genome
            shotgun sequence.
ACCESSION   DTVD01000180 DTVD01000000
VERSION     DTVD01000180.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_6676_cov_585.999547"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="EYO68_06365"
     CDS             <1..163
                     /locus_tag="EYO68_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC07462.1"
                     /translation="IPRGGKSNALTEAIITWQVKGKRLQTIGIDSDQVISAVNATLKM
                     LNLQLLQKK"
     gene            260..1561
                     /locus_tag="EYO68_06370"
     CDS             260..1561
                     /locus_tag="EYO68_06370"
                     /inference="COORDINATES: protein motif:HMM:PF01467.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07463.1"
                     /translation="MSPITAEDKLSTIFFPLTANPAGNHHLLLVESVLQQFPETKLVV
                     FLLSNGLHPDPFKHQKIPPAALRLEMLRSALADWTDPEKSLPAQIAEEAGTSLKLNPN
                     NCAISRCELSLNRPLRFVEHLKNISGTEKIPMIVGADLIERMLNPQIFTTVDLKEIEK
                     GCHLLAAPRNNIELESILQLVKQKRGVTLTVTHIMPKAIAPNLQKFLLISSTLIRRAT
                     QAGHVLEAFLPKNAARLIQQNSLYDGSSHVFNFQTVNMNELQLRCSELERQLEEAAKK
                     LQKLLDQLETQNRAHRFAVVETSAGGQIAEGCTSKSGASQHFLAGRVLYSLEAQKQFL
                     GRKFAENSSLSDKQVRQLAKVMQKESGADWVLAETGMAGPPSPERRSKKNGQCHLGLA
                     LSSEVKYKYLELNPFLTRKEHQLLFAIEALIWAESVLKEHN"
     gene            1571..2593
                     /gene="trpD"
                     /locus_tag="EYO68_06375"
     CDS             1571..2593
                     /gene="trpD"
                     /locus_tag="EYO68_06375"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIC07464.1"
                     /translation="MRELLQKLLAAEELSFEEAREMILWIMSEDAVSVQAAALLALLQ
                     AKGVTVEEMAGAALAMRERASAIAAPKNAIDTCSTGGNGISTFNISTCAAIIAAAAGA
                     VVAKHGNRSNTRKSGSAEALEALGVNISLELKEVESCLKALDICFCYAINHHPAMRFA
                     GPIRKDLGIPTIFNLLGPLTNPAAARSQLIGVPNKELTLKIAQVLKRLGSNRVLVVHG
                     DDGLCELTVTAPTHVAELRDGKIREYDVTPDELGFKQGNLADIGIDSPEESAKIIERI
                     FSGADTGTARDIALANAAGALVVSGLVDELREGVELAAKTVDSGKAEEKLKNLIKFSQ
                     ANHQQA"
     gene            complement(2674..3474)
                     /locus_tag="EYO68_06380"
     CDS             complement(2674..3474)
                     /locus_tag="EYO68_06380"
                     /inference="COORDINATES: protein motif:HMM:PF03009.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIC07465.1"
                     /translation="MENIKTLEQIKRPWVVAHRGYRGSYPENTLVAFEAAIGVNADMI
                     ELDVSLTRDRIPVVIHDNTLDRTTNGSGPVSEHTLAELKELDAGSWFSTKFSGEAIPT
                     LEELLMSVKGRITVNIEIKPESFESPAIPDGIESQICELVEKLEMGDSVLISSFEHFF
                     FARIQRWYRNQGKSTLPKVAPLQEAPLSEELALGLCSRQGAYSFHPDENFVTPSLIET
                     LKADGFRIFPYTINKEKRMEQLIQWGVTGIISDEPELVWKVIRKLGVD"
     gene            3590..4495
                     /locus_tag="EYO68_06385"
     CDS             3590..4495
                     /locus_tag="EYO68_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07466.1"
                     /translation="MFELKDLTDDNDFNASDYRLNPREFFEKRRTSKRPYVYDLRSSE
                     AYELENIPGSHNLPIEHFETSIYQMPFAGDILLYGGEDGEVLTAAEILYDNGFDSFCF
                     TDSFEAHLSSAEASYLSITDAAQKQIKDQLQNSDSLTGVQIIVEPTSPLKAKYRIELV
                     ESTAAWSIKLNLKGIYIFSERKTASYLEGTIIEINGEGELEPRNPQLSISKLSGSLEE
                     QIQLMLDEQVNPMLASHGGNVMLEGIKDSTAYVRFGGGCQGCSMIDTTVKQGVEVMLK
                     EAIPDLAGVYDVTDHSEGESPFFTG"
     gene            4574..4996
                     /locus_tag="EYO68_06390"
     CDS             4574..4996
                     /locus_tag="EYO68_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07467.1"
                     /translation="MSSSSKSISNNDKTSVLEDEEEFSLVVSKGRDLLENKTEFQTDD
                     WVWTRDLDDGGIFFFCYLLIDYRQQTLNKNSLRESVHTLNLLLHKMLPPRDKTGLPLL
                     GEFQVIFTLYERLKREEMTWDDCEKYIMEQISEHQNSN"
     gene            complement(5003..6040)
                     /gene="plsX"
                     /locus_tag="EYO68_06395"
     CDS             complement(5003..6040)
                     /gene="plsX"
                     /locus_tag="EYO68_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIC07468.1"
                     /translation="MAIAVDAMGGDSPLSVQVEAAVRAVNDFGIDVVLVGAFEQIEET
                     LKLHNYDSDKLRIVNSTETVEMNESPASALRQKKHSSIRVAVDLVKAGEAEAVVSAGN
                     TGVSMATAKFILKSIEGIERPAIASIMPSVHRNHPFVLLDVGANTDCKPLYLFQFALM
                     GDAYARTYLHLENPRVALLNNGEEEGKGYLELQETYDLLKQSTLNFVGNIEGKAMFRD
                     KVNVVVCDGFVGNIALKVAEGTFDFVRSILREEVKNSLLSKFGYLAMKGPFKALRQRA
                     DYSEVGGASLLGINGIVIICHGNSKVHTFRNAIKHAQECVDLELNDKISAEVSENLEV
                     INQAKTLNGGE"
     gene            complement(6089..6271)
                     /locus_tag="EYO68_06400"
     CDS             complement(6089..6271)
                     /locus_tag="EYO68_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002831400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIC07469.1"
                     /translation="MAVPRRKTSRSQRDHRRGHIKIPESALSECTNCHALIRPHRVCP
                     ECGFYKGVEVIEIKAS"
     gene            complement(6371..>6676)
                     /locus_tag="EYO68_06405"
     CDS             complement(6371..>6676)
                     /locus_tag="EYO68_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIC07470.1"
                     /translation="YTVTANCEKCKYTDCVEVCPVEAFHEGPDMLYINPEACIDCNAC
                     VEECPVEAIYADVDVPEKWESYTALNEEKCEEYPIIEEAKDPLPTARTLEEIQAAEG"
BASE COUNT         1968 a         1410 c         1405 g         1893 t
ORIGIN      
        1 gattccacgc ggcggaaaat cgaatgctct cacagaagca attatcacct ggcaggttaa
       61 aggaaaacgt cttcaaacaa ttggaattga ttcagatcag gttatttcag ccgtaaatgc
      121 aacccttaag atgctcaatt tgcaactgct gcaaaaaaaa tagctgaacg ctaagtccaa
      181 agtgagttga ttatttgcaa ttcagttcct tttctgagtg agcaccaacg ccttcctgca
      241 tgctttggag tttaaacaca tgagtccgat aactgctgag gacaaactgt ctacgatttt
      301 tttcccctta actgcaaacc ctgccggtaa ccaccatttg ttattggttg aaagcgttct
      361 gcagcaattc cctgaaacta aacttgttgt atttttgctt tccaacggac tgcatccgga
      421 tccattcaaa caccaaaaaa tccctcctgc agcattacgc ctggaaatgt tacggagtgc
      481 actggcagac tggactgatc ctgaaaaatc actgcctgca caaattgctg aagaagccgg
      541 aacatcccta aaattaaatc cgaataattg tgcaatttcg cgctgcgaac tatctctcaa
      601 tcggccactg cgttttgttg aacatctaaa aaacatttcc ggaacagaaa aaatcccaat
      661 gatagtaggc gcagatttga ttgaacgcat gctaaatccg cagattttca caacagtgga
      721 tttgaaggaa atagagaaag gctgtcactt actggccgcg ccgcgcaata acatagaact
      781 tgaatcaatt ctgcagttgg taaagcaaaa acgtggtgtt actttaacag ttactcacat
      841 tatgcccaaa gccatagcac caaatctgca aaaattctta ttgatttcat cgactctaat
      901 ccgcagagct actcaagcag gacatgttct tgaagctttt ttaccgaaaa atgctgcgag
      961 actaatacag caaaatagtt tgtacgacgg ttcctcccat gtctttaatt ttcagactgt
     1021 aaacatgaat gaacttcaac tgcgctgttc tgaattggag aggcaattag aagaagctgc
     1081 aaaaaaactg cagaaattgc ttgatcaact ggaaacacaa aacagagcac accgctttgc
     1141 tgttgtggaa accagtgctg gcggacaaat cgcggaaggt tgcaccagca aatcaggagc
     1201 ttctcagcat tttcttgcag gccgagttct ctatagtctg gaagctcaaa agcaatttct
     1261 gggacgaaaa tttgctgaaa attcgagtct atctgataaa caagtccggc aactggccaa
     1321 agtaatgcaa aaagaatccg gagcagactg ggtactcgca gaaacaggca tggcaggtcc
     1381 tccttctccg gaaagaagaa gcaaaaaaaa tggacaatgt catcttggac tggcattatc
     1441 ttcagaagtc aagtacaagt atctggagtt aaatcctttt ttgacgcgaa aagaacatca
     1501 gttattgttt gcgattgagg ccctgatctg ggctgaaagt gtgcttaaag aacataatta
     1561 aaattttcag atgagagaat tactgcaaaa attactggct gctgaagaac ttagttttga
     1621 ggaagcacgg gaaatgattc tttggattat gagtgaagat gcggtttctg tgcaggcagc
     1681 agctttgttg gctcttttac aggcaaaggg tgtgacagta gaggaaatgg caggtgcagc
     1741 tctggctatg cgagaacggg catccgcaat tgcagcacca aaaaatgcga ttgacacgtg
     1801 cagtaccggc ggaaatggaa tcagtacttt taatatttct acttgcgctg caatcattgc
     1861 agcagcagct ggtgcagttg tcgctaaaca cggtaaccgc agcaacacac gtaaaagcgg
     1921 aagcgctgaa gctctggaag ctttgggagt aaatatttct ttggaattaa aggaggtcga
     1981 aagctgtctc aaggcattgg atatttgttt ttgttatgca ataaatcatc atcccgccat
     2041 gcgctttgcc ggcccaattc gtaaagatct gggaatacca acaatcttta atttacttgg
     2101 cccacttacc aatcctgctg cagccaggag tcaactcata ggtgtgccga acaaagaact
     2161 gaccttgaaa atcgcccagg tactaaaacg gctaggcagt aacagagtat tagtggttca
     2221 tggagatgat ggactttgcg aactaacagt tacagcacca actcatgtgg ctgagcttcg
     2281 tgacggtaag atccgtgaat atgatgtaac tccggatgag ttgggattca agcagggtaa
     2341 tttggctgat attggcatag acagtccgga agaaagtgcg aagattattg aacgaatctt
     2401 ttccggagcc gatacaggaa ctgcacgtga tattgccctg gcaaatgctg caggagcatt
     2461 agttgtttct ggcctggtgg atgagttacg ggaaggtgtt gagctggcgg ctaaaaccgt
     2521 ggacagtggt aaggcagaag aaaaactgaa aaatttgata aaattctccc aggcaaacca
     2581 ccaacaagct taaaagaggt tgtttctcag tcccgcctta tttgaatagt agtgtgtttg
     2641 tttgatcatc taaatgtgta ctgttttatt ggctcagtcc acccccagtt tgcggatgac
     2701 tttccacacc agttcaggtt catcactaat aattccggta acaccccact gaatcaactg
     2761 ctccatccgc ttctctttat tgatggtgta tgggaaaatt cgaaagccgt ctgctttcaa
     2821 tgtttcaata agagatggag taacaaaatt ttcatcagga tgaaaggaat atgcgccttg
     2881 tcgactgcag aggcccaagg ctaattcctc tgaaagaggt gcttcctgta atggtgcaac
     2941 tttgggaaga gttgacttgc cctggtttcg ataccacctc tggattcttg caaaaaagaa
     3001 atgctcaaaa cttgaaatca atacagaatc acccatctca agcttttcca ccagttcgca
     3061 aatttgtgat tcaattccat ccggtatagc aggagattca aaactttccg gtttgatttc
     3121 gatattgact gtaattcgcc ctttgactga catcagcagt tcctcgagag tcggaatagc
     3181 ctctccgcta aattttgtac tgaaccaact gcctgcgtcc aattctttta attcagccag
     3241 tgtatgttct gacactgggc cagagccatt ggttgttcta tccaaagtat tgtcatgaat
     3301 taccactgga attcgatcac gtgtaagact tacatcaagt tcaatcatat ctgcattcac
     3361 tccaattgct gcctcaaaag caactaaagt attttcagga tatgagcccc gatatcctct
     3421 gtgagcaacg acccaaggtc tttttatttg ctcaagtgtt tttatatttt ccatagattg
     3481 acacctttgt gcagttggta taaaatttta caaataatat tttatccaca tgagtcaaag
     3541 cgcccagaat atcgttcctg gcgaaaaact caattcaaat ggagtgttca tgtttgaatt
     3601 aaaagatctg acagatgata atgattttaa tgcgtcggat taccgcctga atccccgtga
     3661 attttttgag aaacgccgca cttccaagcg gccctatgtt tacgatttgc gcagttctga
     3721 agcatatgag ttggaaaata ttccaggttc acataatttg cccattgaac attttgaaac
     3781 ctccatttat caaatgcctt ttgcaggaga tatcctgctt tatggcggag aagacggaga
     3841 agtccttact gcagcagaaa ttctctatga caatggcttt gactcgttct gttttacaga
     3901 ttcttttgaa gcacatttga gcagcgcaga agcttcatac ttgtccatca cagatgctgc
     3961 ccaaaaacag attaaagacc aactccaaaa ctctgattca ctgactggtg ttcaaataat
     4021 agttgaaccg acctctccgt tgaaagcaaa atatagaatt gaactggtgg aatcaacagc
     4081 agcatggagc ataaagttaa atctcaaagg aatttatata ttttcggaac gaaaaacggc
     4141 ttcgtatctg gagggaacaa ttattgaaat taacggtgaa ggagagctgg agccgaggaa
     4201 tccgcaactc tcaatcagca aattaagcgg ttccctagaa gaacagatac aactcatgtt
     4261 ggatgaacaa gtcaacccga tgcttgcttc acatggtgga aatgtcatgc ttgaaggaat
     4321 taaggacagc actgcatacg tgcgttttgg tggtggttgt cagggctgca gcatgatcga
     4381 tactactgtt aaacagggag tagaggtgat gctcaaagag gccatcccgg atctggccgg
     4441 agtatatgac gtaacagacc actcagaggg agaatctcca tttttcactg gttaattctt
     4501 ctgtagtttc aaaaaatatg gaagcggtta tccaataata cttctttaca tcatccaaat
     4561 cacctgcctt ttcatgtcct cctcttcaaa aagtatttca aataatgata agacttcagt
     4621 tttggaagat gaggaagagt tttcactggt agtcagcaaa ggaagagact tgcttgaaaa
     4681 caagaccgag tttcaaacag acgactgggt ttggactcga gatttagatg atggcgggat
     4741 ctttttcttt tgctaccttt tgattgacta caggcaacaa acactgaaca aaaacagctt
     4801 gagagaaagt gtccatactc tgaatctcct gctgcacaag atgcttcctc cacgagataa
     4861 gacaggattg cctttgcttg gagaatttca ggtgattttt acgctttatg aaagattgaa
     4921 gcgcgaagaa atgacatggg atgattgtga gaaatatatc atggaacaaa tttcagagca
     4981 ccagaatagc aattaagata gcttactctc ctccgttaag agtctttgcc tgattgataa
     5041 cttcaaggtt ttcagacacc tcagcagaaa ttttatcatt tagttctaaa tcaacacatt
     5101 cttgtgcatg ttttatggca ttcctgaatg tatgcacttt tgaatttccg tgacatataa
     5161 ttacgattcc atttattcct agtaaggagg caccgccaac ttccgaatag tcagcccgtt
     5221 gtcgcagtgc cttgaatgga cctttcattg ccagatatcc aaactttgaa agtaatgagt
     5281 tttttacctc ttcgcgaagg atactgcgta caaaatcgaa agtaccttct gcaactttga
     5341 gtgcaatatt tccaacaaaa ccgtcgcata ctacgacatt cactttgtct cggaacatgg
     5401 ccttaccctc aatgtttcca acaaaattaa gcgtactttg tttcaggaga tcataggttt
     5461 cttgaagttc aagatagccc tttccctctt cttcaccatt gtttagcaaa gcaactcgcg
     5521 gattttccag gtgcagatac gtgcgtgcat acgcatcacc cattaaggca aactgaaaaa
     5581 ggtaaagggg tttgcaatct gtgtttgccc ctacgtccag aagaacaaag ggatgattgc
     5641 gatgaacaga cggcatgatt gaagcaattg ccggacgctc gatgccttca attgatttga
     5701 gaataaactt tgcggtagcc attgagacac cggtatttcc tgcactgaca actgcttcag
     5761 cctcaccggc ttttacaaga tcaactgcta ctctgataga tgaatgcttt ttttgacgca
     5821 aagctgaggc aggagattca ttcatttcca ccgtttcagt ggaattaacg atacgtaatt
     5881 tatcagaatc gtaattgtga agcttcagcg tttcttcaat ttgttcaaac gctccaacca
     5941 aaacaacatc aatgccaaag tcattgacgg ctcttacggc agcctcaacc tggaccgata
     6001 agggactatc tcctcccatt gcgtcaactg caattgccat actgtttccc gggttgctgc
     6061 tgatgagtga aagagctgaa aaaacagctc aagatgcttt aatttcgatg acttcaacgc
     6121 ccttataaaa tccacattcg gggcaaactc tatgaggacg gatcaatgca tgacagttgg
     6181 tgcattctga gagggcagat tctggaattt tgatgtgtcc ccgtctgtga tcacgttgac
     6241 tgcgcgaggt ttttcttctt ggtacggcca tggttcgttt gtgtttgtgt taaaattcta
     6301 ttttgatcaa tgcttgccat taaatgctga taacgttttt tttaaaaaca aaatcagctt
     6361 tttgttcagc ttaaccttct gcagcctgaa tttcttcaag agtacgtgca gtagggagag
     6421 gatcttttgc ttcttcgatt attggatact cttcgcattt ttcttcattt aatgcagtgt
     6481 aactttccca tttttccgga acatccacat ctgcataaat tgcttccacg ggacattctt
     6541 caacgcaggc attacaatca atgcaggctt ccggattaat gtacaacata tcaggccctt
     6601 cgtgaaatgc ctctacagga catacttcca cacagtctgt atatttacat ttttcacagt
     6661 ttgcagttac agtata
//
LOCUS       DTVD01000181            6631 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3546_length_6631_cov_254.612530, whole genome
            shotgun sequence.
ACCESSION   DTVD01000181 DTVD01000000
VERSION     DTVD01000181.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_6631_cov_254.612530"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="EYO68_06410"
     CDS             complement(<1..189)
                     /locus_tag="EYO68_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIC07471.1"
                     /translation="MREALQQQGHELKEPEPHGLIPVEAVHHQDYLHFLKNIYRRWQE
                     IPGASAEVIPNIHPNRTAG"
     gene            complement(329..2041)
                     /locus_tag="EYO68_06415"
     CDS             complement(329..2041)
                     /locus_tag="EYO68_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HIC07472.1"
                     /translation="MYTYHPVVEILVKSFGTTPQSLEPLHEQLLAYRENTEGLKEAML
                     KHKVTGESEFQQALAVYYELEYRDNFNDVPVVREFTEFIPIRYAKKNIFFPLKKTRNT
                     LEVAITNPELSQPLDDLARHFKCHIQSVISHKQAVLDLINRAYDESSASTEDAVDLLD
                     SEETYENILAVEEPEDLLDATDEEPIKRLVNSLLWQAAKDEASDVHIDPTPRETIVRY
                     RIDGVLQQVTVFPRQVHVTVVNRIKVMSRLDIAQKGLPQDGRSMVLIAGRKIDIRVST
                     VPTVHGEKIVMRLLFQEEKLMQLRHLGLAKYILQPYQNMVNSSGGIILVTGPTGSGKT
                     TTLYASLAEIDNEARNIITIEDPVEYKLSGYSQIEVKPKVGLTFANALRSVLRQDPDV
                     IMVGEMRDTETAQIAIQSALTGHLVFSTVHTNSAPATITRLIDMGIEPFLVSSSIIGV
                     LAQRLVRRICPDCRKSYQPHPEQLRELGIKEVSFRKLDRRFFRGDGCDNCRQTGYRGR
                     IGIHELLVMSEGVKNTILESSDSDTIKKQGLKEKMITLRRDGVNKILHGLTTAEEILT
                     ITSE"
     gene            complement(2270..2776)
                     /locus_tag="EYO68_06420"
     CDS             complement(2270..2776)
                     /locus_tag="EYO68_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07473.1"
                     /translation="MKYITWFLTSILLFQSTVSGVLAAKINQSEKPPAVTVKNPFRSL
                     LLENKDLTSLTKSPSKPGTSRKKYSSQKKKQVLKQNVIAGVQEKPLIQKADFSLRVKE
                     LFQNLKLKDILETSEGRYALIDENKVTYFVQQGEWLGEVFVKHIDKNQVELKEIYGET
                     TIILELKP"
     gene            complement(2800..3375)
                     /locus_tag="EYO68_06425"
     CDS             complement(2800..3375)
                     /locus_tag="EYO68_06425"
                     /inference="COORDINATES: protein motif:HMM:PF04350.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07474.1"
                     /translation="MNSASVTDIFGTIVNNNRSRQVLLAGLIVFLTVLLFHNFILAPE
                     LKSIKQLKSQILQIENSLIQVNAANNNSKKPNLVQSLEKRETALDKLLPPQWKRAELL
                     RWLTDSIQKNGLIFEEQTLEEKTPQALNQVLKIRLKLKGEFEQLLQFLNQLKSGPRLL
                     VLEKLQLENTTPALPEPKLSIEMILSAHRRL"
     gene            complement(3384..4394)
                     /locus_tag="EYO68_06430"
     CDS             complement(3384..4394)
                     /locus_tag="EYO68_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07475.1"
                     /translation="MQLLLAICLVTLLTGGMPLIAFSQNLSLQSKIRLKGQVQLPSNV
                     VMPEGKLDVVLLKFVLSSEGQVTPTGPQARVKTDAEGNFEFLNIIPDLRAGYQIGTRV
                     EGKLYSSKVFFIKAGETLIQKNIIIPGISTAVDKLETYRVSLVIESGLGAVTVTEVLA
                     LSNSSADRIDTGNQSLKQKLPEGIENFRMMETNSGAVIQHYLEDNILIIEHVFPTGNS
                     QIIYQYLLPGWFGSLEMNREFNLSLDKVDVLTPEGYLQIKSEQLIFSDKQSFHDITYL
                     TWKTKASDSNLLTFTISNVPVPSLQYSVVSGVVLLLLFTTVALFFQFRLNNKKRSEES
                     TS"
     gene            complement(4475..>6631)
                     /locus_tag="EYO68_06435"
     CDS             complement(4475..>6631)
                     /locus_tag="EYO68_06435"
                     /inference="COORDINATES: protein motif:HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07476.1"
                     /translation="DKTHVGEVQYQLGFLFTERKQQKKAIKIFENYLAGDNEEHREEI
                     QLRLGYLYAENKQQKKAIKIFEKYLASGAVGHIAETQLSLGYLYVENKKPFLAIAVLE
                     QVLLHPDYQKNNNLLQTLLVLYRENVSEEKYLQFLLKVKNDPKQGGKVQQGFKSQLII
                     EYFEQNKCDKLISELNPDYMQSIKKSNTKEWEHLLYMRGSCLIETKRWNEARIDLRQI
                     RDTKKYREHSIQMLIVTHKQLEDWKRLTWEYQEVYDKKTPVLTIADYQLWVFAAKQRK
                     DFQRLERIKIIYERWKKAFPEDSQNLEQLNKYISGFRLQELTSQENWKEVSAYIKKEV
                     QSGSVSLNEQYFSQLLFAENKLENWSGVLGAYALLRKHDQKRAKTLDALINQAKAADK
                     LGEKELSLKYYRQALKVKPLNQEDKKRQAEIKTFLAQSSFRKWIEQGEWTKISKTIRK
                     EVAENKRVLNDTNFDLLLYTENQKSGNKKYNGILDAYALLATYDKKKTLTVKAQIDQG
                     YAAAKLGGYKRAKGYYRRALKKTPDKDVDLVLQLVGELKRLYERSKDYASLVVIYKRA
                     YSVLKKSSRPKNESRTYAYLIGYHQSFHLKQNDKARIWLMRSDGGGSTPQELDAAFWV
                     AKLDREANKPGMAIKRLKELAGRKVSKNSSLYVQIHFELGTLYHLKEKWKSALLHYRL
                     VAKARAPAELKQYQNTAKEKAKEISSYLKSLNSSEG"
BASE COUNT         1738 a         1346 c         1317 g         2230 t
ORIGIN      
        1 tccggcagta cggttaggat gaatatttgg aatcacttct gcggaagccc ctgggatttc
       61 ctgccagcgc cgatagatgt ttttgaggaa atgcagataa tcctggtgat gtacagcttc
      121 aacaggaatt aacccgtgag gttccggttc tttgagttca tgcccttgct gctgcagggc
      181 ttccctcaaa accgttgccc tttctgctac ttcagaattt ggtttgggtg caccgctgaa
      241 aagggaaaat ttgggttcat gtttgaattg ctccgggtca aaaaatgcga gcatgatttt
      301 acagaactaa aaatttttag atataaattt actctgaagt tatcgtcaaa atttcttcgg
      361 cagttgttaa accgtgtaga attttgttta ctccatcacg ccgtaaggta atcatttttt
      421 ctttcaagcc ttgttttttt atggtgtcag agtcgctgct ttcaaggata gtatttttta
      481 caccttcgct cattaccagc aattcgtgaa ttccaatccg tccgcggtag ccggtttgtc
      541 ggcaattatc gcagccgtca cctctgaaaa atcgtcggtc cagttttcgg aagctgactt
      601 ctttaatgcc taattcacgg agttgttccg gatgcggctg gtagcttttg cggcagtccg
      661 gacaaatacg ccgtaccagc cgttgtgcaa gtacgccgat gattgatgat gaaacgagga
      721 atggctcgat acccatatcg atcaggcggg tgattgtagc gggagcgctg ttggtgtgta
      781 ccgtgctgaa aaccagatgt ccggtcaatg cggattgaat ggcaatttgt gcggtttctg
      841 tgtcacgcat ttctcctacc ataataacat ctggatcctg ccgcaaaact gaacgcaaag
      901 cgtttgcgaa agtcaaaccg actttgggct taacttcaat ctgactgtag cccgaaagtt
      961 tgtattcaac tggatcttca atggtgatta tattacgggc ctcattatct atttcagcaa
     1021 gagaagcata aagggtagtg gtttttccac tcccggtagg tccggtaaca aggatgatgc
     1081 cgccactgct gtttaccata ttttgatatg gttggagtat atattttgcc aaaccaagat
     1141 gacgcagttg catcaatttt tcttcttgaa acaataggcg catgacaatt ttctctccat
     1201 gtacagtcgg tacagtcgaa acgcgtatgt caatttttct tccggcaatc agtaccattg
     1261 aacgaccgtc ctgcggcagg cctttttggg caatatcaag gcgagacatg actttaatcc
     1321 ggttaaccac cgttacatgt acctgacgtg gaaaaactgt tacttgctgt agcaccccgt
     1381 caattcgata acgaacgatg gtttcacggg gtgttggatc aatgtgaaca tcgctagctt
     1441 catcttttgc cgcctgccaa aggaggctgt tcactaaacg tttgatgggt tcttcatcag
     1501 ttgcatccag caaatcttcc ggttcttcaa cagcaagaat gttttcatag gtttcttctg
     1561 aatcaagcag gtcaacagcg tcttcggttg aagcagaaga ttcatcataa gcacgattga
     1621 tcaaatcgag cactgcctgt ttgtgtgaaa taaccgactg aatatggcac ttgaaatgtc
     1681 gggccaggtc atccaaaggc tgtgagagtt ccgggtttgt tatggctact tccagtgtat
     1741 ttctagtttt tttcagtgga aagaatatat tttttttggc atagcgtatc ggtatgaatt
     1801 ccgtaaattc acgtacaacc ggaacatcat taaaattatc tctatattcc aattcataat
     1861 atacagccaa tgcctgctga aactctgatt caccggttac tttgtgttta agcattgctt
     1921 ctttcaagcc ctccgtattt tcacggtagg ccagcaactg ttcgtggagt ggttccagtg
     1981 actgcggagt tgttccgaag ctcttcacca aaatttcaac aaccggatgg taagtataca
     2041 tttcgtatta tttatttaac ttgtgaaagt gttcgtgagt catgaattct cataagatac
     2101 tgatattatt aactgtgata tgtcatctcg aacgaaggga gagatctgat aaagtgctga
     2161 taccttataa gatccctcac atactttcgg attgatacaa taaaactcgt gactttcgag
     2221 taattgagta gaacaaaaac caactcaatt cggattcaac acaaaataat catggtttca
     2281 gttccagaat aatggttgtt tctccgtaaa tttcctttag ttccacttga tttttgtcta
     2341 tatgcttgac aaaaacctca cccagccact ctccctgctg tacgaaatac gtgactttat
     2401 tttcgtcaat taaagcataa cgaccctctg atgtttcaag aatatccttc agtttgaggt
     2461 tctgaaataa ttcttttact ctcaaagaaa aatctgcttt ttgtataagc ggtttttcct
     2521 gcaccccggc aatcacattc tgtttcaaga cttgcttctt cttttgggaa gaatatttct
     2581 ttctggacgt accaggtttt gaaggtgact tagtgagtga cgttaagtct ttattttcca
     2641 gcagcaaact gcgaaaagga tttttgactg taactgcagg gggtttctcc gactggttga
     2701 tttttgcagc aagcactccg gatactgtag actgaaaaag caagattgat gtcaaaaacc
     2761 acgttatgta cttcatggac ttttttgtgt tgcagggatt tacagtcttc tgtgggcaga
     2821 taaaatcatt tcaatactca gttttggttc cggcagggca ggtgtagtat tttcaagctg
     2881 aagtttttcc agcaccaata atctcggtcc gctttttagt tggttaagaa attgtaggag
     2941 ttgctcgaat tctcccttca gtttcaatcg aattttcaaa acctgattca aagcttgggg
     3001 tgttttttct tccaaagttt gttcttcaaa aatcaatcca tttttctgaa ttgagtcagt
     3061 gagccagcgt aacagttcag cacgcttcca ctgcggtgga agcagcttat ccaaagcagt
     3121 ttctcttttc tccagagact gaacaagatt aggtttttta gaattattgt tggcagcatt
     3181 tacttgaatc agactatttt ctatctgcag aatttgagat ttcaattgct ttatactttt
     3241 taactccgga gccaatataa aattgtgaaa caacaacact gtcaggaaca caatcagccc
     3301 cgccaataaa acttgacgac tccggttatt attcacaatt gtaccgaaaa tatctgtaac
     3361 tgaagctgaa ttcatgtcta ttgtcaggaa gtagattcct cagaacgttt tttgttgttt
     3421 aaccggaatt gaaaaaacaa ggctactgtt gtaaataata aaagtagtac tactcctgaa
     3481 actacggaat actgcagcga aggtacagga acattgctga ttgtaaatgt caacagattt
     3541 gagtctgagg ccttggtttt ccatgtgaga taagttatat catgaaaact ttgtttatcg
     3601 gaaaaaatga gttgttccga cttaatctgt aagtatcctt caggtgtcaa aacgtcaact
     3661 ttatccaggg agaggttaaa ttccctgttc atttccaaac tgccaaacca gccaggtagg
     3721 agatattgat aaataatttg tgaatttcct gttggaaaaa catgttcaat tattaaaata
     3781 ttatcttcca gataatgttg aatcacagct ccggagttgg tctccatcat cctgaaattt
     3841 tctattcctt caggtagttt ctgtttcaaa gactggtttc ctgtatcaat tctgtctgca
     3901 gaggaatttg atagtgccag aacttcggtt acagtaacgg ctcccagtcc ggattcaatc
     3961 acaagtgaaa ctctatatgt ttccagttta tcgacagcag tagaaatccc tggaatgata
     4021 atgttttttt gaattagtgt ttctccggct tttataaaaa atacttttga actgtataat
     4081 ttaccttcta cccgagtccc aatttgatag cctgcgcgta aatcagggat aatattgaga
     4141 aactcaaagt tgccttcagc atcagttttt acccgtgcct gtgggcctgt tggagttacc
     4201 tgtccttccg aacttaagac aaatttcaat aacaccacat caagtttgcc ttcgggcatt
     4261 accacattgg atggaagttg tacctggccc ttcagacgga ttttgctttg cagggacagg
     4321 ttttgtgaaa acgcgattaa gggcattcct ccagtaagta aagtcaccag acaaattgcc
     4381 aatagaagtt gcatgtattt attttgtctg tgttttacaa tctcgtaaaa aacacctgaa
     4441 ctgtatacag ccgatttatt attatcgtac attttcaacc ttcagaagaa ttaagagatt
     4501 ttaaatagct gcttatttcc tttgcctttt ccttggcagt attttgatac tgttttaatt
     4561 ctgctggtgc acgggctttg gctacaagtc gatagtgcag taaagcggat ttccactttt
     4621 cttttaaatg atacaatgtt ccaagttcaa agtggatctg aacataaagt gaggagtttt
     4681 ttgaaacttt ccgaccagcc agttctttga gtcttttgat agccattccc ggtttgtttg
     4741 cttcacggtc aagctttgct acccagaatg cagcatccaa ttcctggggc gtggagccac
     4801 ctccatcaga acgcatcagc caaatacggg ctttgtcgtt ttgtttaaga tgaaatgact
     4861 gatggtagcc gatgaggtag gcgtatgttc gggactcatt tttgggccgg gaacttttct
     4921 tgagaacact atatgcccgc ttgtaaatca ctaccaaaga cgcataatcc ttggatcgtt
     4981 cataaagccg tttaagttct cctaccagtt gcagcaccag atcaacatct ttgtcaggag
     5041 tctttttgag agcccttcta tagtatcctt ttgcacgttt atatcctcct aattttgctg
     5101 cggcatatcc ttggtcaatt tgagctttta cagtaagtgt cttttttttg tcataagttg
     5161 ccagcaacgc ataagcatcc aggattccat tatatttttt gtttccggac ttttggtttt
     5221 ctgtataaag cagcagatca aaattggtat cattcaaaac tctcttgttt tcagcaacct
     5281 ccttgcgtat tgttttgctg attttagtcc actctccctg ttcaatccat tttcggaaag
     5341 aactttgtgc cagaaacgtt tttatttctg cctgccgctt tttatcttct tgattgagag
     5401 gttttacttt taaagcctgg cggtaatatt tcagcgagag ttctttttcc ccaagtttat
     5461 ctgcagcttt tgcctggtta atcagtgcat ccagtgtttt agcacgtttt tggtcatgct
     5521 ttcgtaaaag agcataggca cccagtacgc cggaccaatt ttccagttta ttttctgcaa
     5581 agagcagttg cgaaaaatac tgttcattaa gcgaaacaga accggactga acctcttttt
     5641 tgatatatgc agaaacttcc ttccagtttt cttgtgaagt aagttcctgt agacgaaatc
     5701 cagaaatgta tttattgagc tgttctaaat tttgtgaatc ttctggaaat gcttttttcc
     5761 agcgttcgta aattattttt attcgttcaa gacgttgaaa gtcttttctc tgtttggcag
     5821 cgaaaaccca aagttgataa tctgctattg tcaaaacagg agtttttttg tcataaacct
     5881 cttgatattc ccaggtcaac ctcttccagt cctctaactg tttgtgtgtc acaatcaaca
     5941 tctggattga atgttcacga tattttttag tatcacggat ttgtcgtaaa tctatgcggg
     6001 cttcgttcca cctttttgtt tcaatcagac aactgcccct catgtaaagt aaatgctccc
     6061 actcttttgt atttgatttt tttatactct gcatgtagtc cggatttaat tctgaaatca
     6121 atttgtcaca tttgttttgt tcaaagtatt caataatcag ctgagatttg aaaccttgct
     6181 gcactttccc cccttgtttt gggtcatttt ttacttttaa aagaaactgc agatactttt
     6241 cttccgagac gttttcccga tagaggacta ataatgtttg caagagatta ttatttttct
     6301 ggtaatcagg atgcaggagg acttgttcca acacagcaat tgccagaaaa ggttttttgt
     6361 tttccacata gaggtaaccc aagctgagct gtgtctcagc aatgtgtcca acagcaccgc
     6421 tggcgagata tttttcaaag atcttaatgg cctttttttg ctgtttgttt tctgcgtaga
     6481 ggtaacctaa tctaagttgt atctcttcac gatgttcttc attatccccg gcgagataat
     6541 tttcaaagat cttaatggcc tttttttgct gttttctttc tgtgaacaaa aaacctagtt
     6601 ggtattgaac ttcacctaca tgtgttttgt c
//
LOCUS       DTVD01000182            1615 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_35794_length_1615_cov_365.063462, whole genome
            shotgun sequence.
ACCESSION   DTVD01000182 DTVD01000000
VERSION     DTVD01000182.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35794_length_1615_cov_365.063462"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="ylqF"
                     /locus_tag="EYO68_06440"
     CDS             <1..898
                     /gene="ylqF"
                     /locus_tag="EYO68_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIC07477.1"
                     /translation="EGPDQLGKGWDTPDINWYPGHMLKAKKELAKQLKRVDVVLEMRD
                     ARIPVSSANHDFEELLQQKKRILLFNKTNLADEDTTRKWKAYFKKMDTPSLFVDALNK
                     QNLQEILPLSRKLMQEKWSNFRKRGIRPPSLKLLITGIPNVGKSSLINRLSRRKAAET
                     GPTPGVTKHQDWIKLGKDVELLDTPGILWPKIENREAGLRLTITGAIKDSIVGTSLLS
                     DYLLGILMLKAPEQLLKHYQLTEENLELLPADLLEKIAEKRGCLKSGGAIDHPRAESL
                     VLRDFRAGKLGRLSFDLPETEE"
     gene            complement(939..1271)
                     /locus_tag="EYO68_06445"
     CDS             complement(939..1271)
                     /locus_tag="EYO68_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIC07478.1"
                     /translation="MKTDFILAVILIAVGTYLIRAVPFVLSSKKETGKQNRHQINLFF
                     KLMGPSMIAALLMVSVESPFEIEDNSQLINTALGFVGVGVSHRLWNNSGISVLAGVVC
                     FTAALLFL"
     gene            complement(1268..>1615)
                     /locus_tag="EYO68_06450"
     CDS             complement(1268..>1615)
                     /locus_tag="EYO68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07479.1"
                     /translation="AFSRIGIMPHESRFWWLMGLEFGAYSCWIGGTLAGSISGDLLLS
                     HFSFLRPALSFALPALFLSLLLPMLNSTTRPAVMISLLLAAAFHWSGHSEIGIIAAAL
                     IGPGIGIIGRNFQ"
BASE COUNT          516 a          344 c          373 g          382 t
ORIGIN      
        1 agaaggtccg gatcaacttg ggaaaggttg ggacactcca gacataaact ggtatccggg
       61 gcatatgctc aaggccaaaa aagagttggc aaaacagctg aagcgtgttg atgtggtgct
      121 ggaaatgcgt gatgcaagga ttcctgtttc ttcggcaaat catgactttg aggaattgct
      181 gcagcaaaaa aaacgaatcc tgctttttaa taaaaccaat ctggcagatg aggatactac
      241 cagaaaatgg aaagcatatt ttaaaaaaat ggacacgccg tccctctttg ttgatgcttt
      301 aaacaaacag aatcttcaag aaatattacc gctttcacgc aaattgatgc aggaaaaatg
      361 gtccaatttc cgtaaacgcg gcattcgtcc tccgtcactg aaactattaa taaccggaat
      421 tccgaatgtg gggaaatcca gcttgatcaa caggctttca agacgaaagg cagccgaaac
      481 agggccgaca ccgggagtga caaaacacca ggactggatc aaactgggca aagatgttga
      541 acttcttgat actccgggta ttttgtggcc aaaaatcgaa aatcgagaag ccggtttgcg
      601 gctgacaatt acgggtgcca tcaaggactc aatagttgga acttccctgt tgagtgatta
      661 tttacttggt attttaatgc tcaaggctcc ggaacagctc ctgaagcatt atcagttgac
      721 tgaggaaaat ctggaattgc ttcctgcaga tttgctggag aaaatagcag aaaaaagagg
      781 ttgcctgaaa agcggagggg caatcgatca tccgcgggca gaaagtctgg tactgcggga
      841 tttcagggca ggaaaattgg gacgtttgag ttttgattta ccggaaacgg aagaataagc
      901 ttttttgcta acacccaaaa ttccggaaat ttcagcggct ataaaaacaa gagtgcagca
      961 gtaaaacaga cgacacccgc aagtacactt attccggaat tattccacaa ccggtgactg
     1021 actcctactc ccacaaaacc cagggcagtg tttatcaact gggagttatc ctctatttca
     1081 aagggagatt ctactgaaac catcagcaat gcggcaatca ttgagggacc catcagctta
     1141 aaaaacagat tgatttgatg ccggttttgc tttcctgttt ccttttttga actgagtaca
     1201 aacggaactg cgcgaatgag ataagttcca actgcaatca gtataaccgc taaaataaaa
     1261 tccgtcttca ttggaaattc ctgccgatga ttccaatacc gggcccaatt aaagctgcgg
     1321 caattattcc tatttctgag tgaccgctcc aatggaaagc tgcagccagg agcaaggaaa
     1381 tcattactgc cggacgcgta gtgctattga gcatgggaag cagtaaactt aaaaaaaggg
     1441 cgggcagtgc aaatgaaaga gctggtctta aaaaagaaaa atgactgagc aacaaatctc
     1501 cgctgatgct tccggctaaa gtaccgccaa tccagcatga atatgctcca aactccaaac
     1561 ccattagcca ccagaaccgt gattcatggg gcatgattcc gattctcgaa aatgc
//
LOCUS       DTVD01000183            6551 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3614_length_6551_cov_568.305727, whole genome
            shotgun sequence.
ACCESSION   DTVD01000183 DTVD01000000
VERSION     DTVD01000183.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_6551_cov_568.305727"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            296..1240
                     /locus_tag="EYO68_06455"
     CDS             296..1240
                     /locus_tag="EYO68_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylesterase"
                     /protein_id="HIC07480.1"
                     /translation="MPSIHPQMQSVLQKSSTLVAPYSITEIPLHQARELYAQERMFWN
                     TGGPNIPSIMETEVKGPVGGIPIRIYQPHPEKCLPVLVYLHGGGFVVGNLDTHDRIMR
                     ELAFRSGCAVIGVEYSLSPEQKFPVALDEIKLVLQWLKEDDYTQKEAAFNIDPDRIVL
                     GGDSAGASLSMGAALSFKEQLSGLLLIYGWFGLRDSCSVRLFGSQDVGMSEEDLAFYQ
                     DSYLNSQEDLNDVRLDVLKADLNGLPPSCLIASDMDPLLDDSTALATLMEQAGVPCEL
                     HLHQGVLHGFLHYSRMLDGAVSALDQSAEFLRKTFCLV"
     gene            complement(1270..2217)
                     /locus_tag="EYO68_06460"
     CDS             complement(1270..2217)
                     /locus_tag="EYO68_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HIC07481.1"
                     /translation="MTNTTLRVLSYNIYWGGHQQPLERTIEVIGESEADLVGIQENVN
                     REYEDQTPEIAKALGFSYVRHAVLDALEKFGAKGNKLRARWTQQAGQSTLSRHPITAQ
                     SPGGSGIQVELPSGEKVWMFNTHLWHYPYVPYQLLNVDFFEGEAIDPNAHDAEAQAIL
                     SARIGHETEINKVLWDIKSILPSGEPVFLTGDFNEPSHLDWTEEAAQAGLYPLKVDYP
                     TSRQVLKAGLKDAFREFYPDPVAVPGFTWPAGIYPDAPDSSIPDPEDRIDFVYFSGAN
                     VRVKKVQRLGNVSDNSEISFAEYPSDHRALLAEFELPEN"
     gene            complement(2407..3516)
                     /locus_tag="EYO68_06465"
     CDS             complement(2407..3516)
                     /locus_tag="EYO68_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HIC07482.1"
                     /translation="MKSRAAIAWEAGKPLEVDMVDLEGPKTGEVLIRNVATGVCHTDA
                     FTLSGDDPEGIFPSILGHEGGAVVEEIGKGVSSVKVGDHVIPLYVPECGECQFCKSGK
                     TNLCQSIRLTQGKGLMPDGTSRFSSGGKTLFHYMGTSTFSEYSVLPQISVAKINTEAP
                     LEKVCLLGCGITTGIGAVLNTAKVEAGATVAVFGLGGVGLGAIQGAVLAKAKRIIAVD
                     INESKEEFARQLGATDFVNPKNYDKPIQEVLVELTDGGVDYSFECVGNVNLMRSALEC
                     CHKGWGESIIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGVKGRSQLPGYVEMYMNGE
                     VKVDEFVSYTMPLDEINRAFDLMHEGKSIRSVILF"
     gene            complement(3624..5447)
                     /locus_tag="EYO68_06470"
     CDS             complement(3624..5447)
                     /locus_tag="EYO68_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HIC07483.1"
                     /translation="MLADANKNSGNEPSATLLEVKGLRIEGLSEKGWQEIVCGIDLKL
                     KRGEILGLIGESGAGKSTIGLAAMGFARDGCRITSGEIIFDGIELCTASEEQKRKLRG
                     NRISYVAQSAAAAFNPAHRLLNQFAEGPVDHKVMSRTEAKEKGRKLYRSLDLPDPDNI
                     GERWPHQVSGGQLQRMMVAMAMSCGPDLIVFDEPTTALDVTTQIEVLKTIKEIVTARN
                     VAAIYITHDLAVVAQLADHLMVLRYGNLIEVNSTGQILEAPKQEYTRALLDARTRHEK
                     TAVQISDAETLLSINSVTAGYGNGPDILQEVSVLVERGRTVAVVGESGSGKSTLARVI
                     TGLLPPRSGEIHFQSESLPPALAQRSKQQLRKVQMIYQIPDVALNPRQRVRDVVGRPL
                     SFYLGLSGKEREHRIIELLEHIELSEHFIDRLPDELSGGEKQRICIARALAAEPELII
                     CDEVTSALDTIVAKAILDLLQRLQRELSLTYLFISHDLDTVASIADQVVVLYAGRIAE
                     QGPKTAVFQPPFHPYTRLLLSSVPEMRQGWLEETLSTREAKAGIAREVKITERGCPFA
                     ERCPVAIPEICVRDRPPLQTPASGHVFACQHAPEMLLDHSA"
     gene            complement(5437..6270)
                     /locus_tag="EYO68_06475"
     CDS             complement(5437..6270)
                     /locus_tag="EYO68_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07484.1"
                     /translation="MSNILKIISSAPLSAKFGMLVVLIYSIVAIFAPFIAPYGEREIL
                     GRAFELWSDKYLLGTDNLGRDMLSRMIFGARNTIGLALSITILAFLLGSLTGMIAAAL
                     GGWVDQILSRIVDVLMSIPSLIFALLILSIFGTSVPYLIATITVIDATRVFRLARATA
                     MNVVVMDYVEVARVRGEKLDWVIRKEILPNITAPLLAEFGLRFCFVFLFISALSFLGL
                     GLQPPAADWGSMVRDNASLITFEDITPLLPAGAIALLTVAINFIVDWMLHKSSGLKDV
                     S"
     gene            complement(6270..>6551)
                     /locus_tag="EYO68_06480"
     CDS             complement(6270..>6551)
                     /locus_tag="EYO68_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC07485.1"
                     /translation="PMKIILRHALPNALAPIVNVVAINLAYLVVGVVIVEVVFVYPGL
                     GQLMVDSVSKRDIPVVQACSMIFASVYILLNLSADIISIVTNPRLLHPR"
BASE COUNT         1738 a         1542 c         1493 g         1778 t
ORIGIN      
        1 cacaaaaata agtgaaagca aggttatttt tgataatcca aaaaaaaatg atgggacttt
       61 tcccggaacc aatgttttga gattctcttt gctttctaca ggtaatcagt aaataataaa
      121 ttttagtact gtgacagatg aagtggtttt tcagtttaag tttcaaatga agacatagtc
      181 cagtaaaagt gtggctgttc tcaacatcag agatactcaa actgtcttag tacaaatacg
      241 cttcataaat ccgaaaaaat tctgactaaa taaccaatta ttttcaccgc cgcttatgcc
      301 ttcaatccat ccgcagatgc aatctgtcct gcaaaagagt tccacattgg tagctcctta
      361 ttcaattact gaaatcccac ttcatcaggc tcgggaactg tatgcgcagg aaaggatgtt
      421 ctggaatacc ggaggtccga atattcccag catcatggaa acagaagtta aagggccagt
      481 agggggaatt ccaatccgga tttatcaacc gcatccagag aagtgtttac cagtgttggt
      541 ttatttgcac ggaggaggat ttgttgtggg aaatctcgac acgcacgacc gcatcatgcg
      601 cgaacttgct tttcgttctg gttgtgctgt gattggtgtg gagtactcct tgtctccgga
      661 acaaaagttt ccggttgcac ttgatgaaat taaattagta ttgcaatggc tgaaagagga
      721 tgattatacg caaaaagaag cagcttttaa tattgatcct gacaggatag tacttggtgg
      781 agattctgca ggagccagtt tgagtatggg ggctgcctta agttttaaag aacaactgtc
      841 cggattattg ttgatatacg gctggtttgg tctgagggat tcctgttctg tccgtctctt
      901 tggaagtcag gatgtgggaa tgagcgaaga agacctggct ttctatcagg attcctattt
      961 gaattcacaa gaggatttga atgatgtacg tctggatgtt ttgaaggctg atctaaatgg
     1021 tctgcctcca tcctgcttga ttgcttctga tatggatccc ctgttggatg acagcacggc
     1081 gcttgccaca ttaatggaac aagccggagt cccctgtgaa ctacacctgc atcaaggtgt
     1141 gctgcatggt tttttacact acagccgcat gttggatggc gccgtttctg cgcttgatca
     1201 atcggcggaa tttttacgaa aaacgttttg tctcgtttga tattattgcg gttaatgatg
     1261 tctggaagtt caattctcag gaagttcaaa ttctgccagt aacgctcggt gatccgaagg
     1321 atattcagca aaagaaatct ctgaattgtc cgaaacatta cctagccgtt gaaccttttt
     1381 cacccgaaca ttcgctcccg aaaaatagac aaaatcaatg cggtcttccg ggtcaggaat
     1441 ggacgaatcc ggagcgtccg gataaatccc cgcaggccaa gtgaatcccg gaactgccac
     1501 agggtcggga tagaattccc ggaaggcgtc tttcaatccc gctttaagta cttgccttga
     1561 agttgggtaa tcaactttga ggggataaag tccggcttgg gccgcttctt ctgtccagtc
     1621 gagatgagac ggttcgttaa aatcgccggt gagaaaaacc ggttctccgc tgggaagtat
     1681 gcttttgata tcccacagaa ctttgttgat ttcagtctca tgaccaattc gtgctgaaag
     1741 aatcgcctgc gcctctgcat catgagcatt cggatctatc gcttcccctt cgaaaaagtc
     1801 aacatttaac aactgatacg gtacataagg atagtgccag aggtgtgtgt tgaacatcca
     1861 gactttctct ccggaaggca attccacctg tataccggaa cctccgggag attgtgccgt
     1921 aatcgggtga cggctcaaag ttgattgccc ggcttgctga gtccagcgtg cgcgaagctt
     1981 gttgcctttg gcgccaaatt tttcaagtgc atccaaaacg gcgtgcctca cataggaaaa
     2041 tcccagggcc ttggctatct ccggagtctg gtcttcgtat tcccggttta cattttcctg
     2101 aatgcccacc aaatcagcct cggattcccc gatcacttcg atggttcgtt caagcggctg
     2161 ctgatgtccg ccccagtaaa tgttataact caaaacccgc aacgttgtgt tggtcatagt
     2221 tcgttatttt tggaggagaa agaattcgtt tttgttcaat tttggttgtc tcgcaaccag
     2281 tctgaaatct taatgattag tgtagcagct catgcaagat ttcttgcttc actcgaaatg
     2341 acacgctttt ggtacttttt acgacaatat aaatattgaa aagagaaaca gttagcttga
     2401 aaaaggttaa aacagaatta ccgagcgaat gcttttacct tcgtgcatca agtcaaatgc
     2461 ccgattgatt tcgtccagcg gcatagtgta gcttacgaat tcatcaactt tgacttctcc
     2521 gttcatgtac atttccacat atcccggcaa ctgcgagcgt cccttaactc ctccaaaagc
     2581 agaaccacgc cagactcgtc cggtaaccag ttggaaaggt cgtgtagaaa tttcctgtcc
     2641 ggctccggca actccgataa ttattgattc gccccaacct ttatgacaac attccagagc
     2701 agaacgcatc aaatttacgt tgcccacgca ttcaaaagaa taatccacac cgccgtctgt
     2761 caattctact agaacttctt gaatcggttt atcgtaattt ttgggattta cgaaatcggt
     2821 tgcgccaagt tgccttgcaa attcttcttt actttcattg atgtccacgg caatgatgcg
     2881 tttggctttg gctaaaacag caccctgaat tgcaccaagc ccaacaccgc ccaacccaaa
     2941 cactgccacc gtcgctccag cttccacttt tgcggtattt aaaaccgccc cgattccggt
     3001 agtaatcccg cagccgagca aacacacttt ttccaaggga gcttccgtat tgattttggc
     3061 gaccgaaatt tgcggcaaaa cactgtattc ggaaaatgta gaagttccca tgtagtgaaa
     3121 caaggttttt cctccggacg aaaaacgcga agtcccgtca ggcatcaatc cttttccttg
     3181 tgtcaaacgt atggactggc ataaattagt ttttccggat ttgcaaaatt gacactctcc
     3241 gcattccgga acataaagtg gaatgacatg gtctccgact ttgacgctac tgactccctt
     3301 gcctatttct tcaacaactg ctccgccttc gtgtccaagg atggagggaa aaatgccttc
     3361 cggatcgtct ccggaaagcg tgaatgcgtc tgtgtgacag actccggtgg ccacgtttct
     3421 gatcaacact tctccggttt taggtccctc gagatcaacc atatctacct ccagaggctt
     3481 tccagcttcc caggcaattg cagctcgtga tttcatactt tcatcctatt aaaaatcttt
     3541 tgaaaaataa taattatttg agactctcac aatccgggta caaattctaa ctgaatttaa
     3601 ataatttgtc tctttattct attttatgca gaatgatcaa gcagcatttc cggagcgtgt
     3661 tgacaggcaa aaacgtgtcc ggatgcaggg gtctgaagtg gtggacggtc acgtacgcag
     3721 atctccggaa tggcaactgg acaacgttct gcaaaaggac agccgcgttc agtaatttta
     3781 acctcacggg caattccagc tttggcttca cgagtggata gagtttcctc cagccagccc
     3841 tgacgcattt ccggcacaga tgaaagcaga agccgtgtgt aaggatggaa aggcggttgg
     3901 aaaacagcag ttttgggacc ttgctctgca atccttccgg catagagaac cactacctga
     3961 tcggcaatac tagccaccgt atcaaggtcg tgactgatga acagatatgt taaactcaat
     4021 tcgcgctgaa gccgctggag caaatccaga attgctttag caacaatggt atccagcgca
     4081 gaagtcactt cgtcgcagat aatcaattcc ggttctgccg ccagtgccct ggcaatgcag
     4141 atacgttgtt tttcgcctcc ggaaagttcg tcgggaaggc ggtcgatgaa gtgttccgaa
     4201 agttcaatat gctccagcag ttcaatgatc cggtgttcac gttcctttcc gctcaaaccg
     4261 agataaaaac tcaagggacg gcccactaca tcccgcacgc gttggcgtgg atttagggct
     4321 acatccggaa tctgataaat catctgaact ttccggagtt gctgtttgct gcgttgggca
     4381 agtgcaggtg gaagcgattc agattgaaag tgtatctcgc cggaacgtgg aggaaggagt
     4441 cctgtaatta cccgggccaa ggtgcttttt ccgcttccgg actctccgac cacagcaaca
     4501 gttcggcctc tctccaccaa aaccgatacc tcctgcaaaa tatccggccc attgccataa
     4561 ccggctgtaa ccgaattgat gctgagcaga gtttccgcat ccgaaatttg tacagcagtt
     4621 ttttcatggc gggtgcgggc atccagcaat gcacgtgtat attcctgttt tggtgcctct
     4681 agaatctggc cggtgctgtt tacttcaatc aggttcccgt agcgcaaaac catcaagtgg
     4741 tctgccaact gtgcgactac cgccaggtcg tgggtgatgt aaatggctgc gacattacgg
     4801 gcggtcacga tttccttgat cgttttcagg acctcaattt gagtcgttac atccaatgca
     4861 gtggttggtt catcaaagac aatcagatcc ggtccgcagg acatcgccat ggctaccatc
     4921 atgcgttgga gctggcctcc ggaaacctga tgcggccaac gctcgccgat attgtccgga
     4981 tcggggaggt caagactccg gtaaagtttg cggccttttt ctttagcttc agtccgggac
     5041 attactttgt gatctaccgg gccttctgca aactggttta gcaggcggtg tgcaggatta
     5101 aaagcggcag ctgcgctttg ggcgacataa gaaatacggt ttccacgcag cttccttttt
     5161 tgttcttctg aggctgtgca caattcgatt ccatcaaaaa ttatttctcc tgaagtaatt
     5221 ctgcaaccgt cgcgggcaaa tcccattgct gccaaaccga tggttgattt tcctgctcca
     5281 gactcaccta tcaaacccag gatttctcca cgcttcagtt ttaaatctat tccgcaaacg
     5341 atttcctgcc aacctttttc tgaaagccct tcaattctga gccctttcac ttccagcaag
     5401 gttgctgaag gttcgtttcc ggaattttta tttgcgtcag ctaacatcct tgagtccgct
     5461 tgatttgtga agcatccagt cgacaatgaa attgatcgca accgttaaca gtgcaattgc
     5521 tcctgcaggc aggaggggtg ttatgtcttc aaaagtaatc agtgatgcat tgtcccggac
     5581 cattgaaccc caatccgctg cgggcggctg aagaccgagt cccaaaaaac tgagtgcgga
     5641 aatgaaaaga aaaacgaagc aaaaacgcag cccgaattct gcgagcagtg gtgctgtgat
     5701 atttggtaat atttctttgc ggatgaccca gtcgagtttt tctcccctga cacgagctac
     5761 ttcaacataa tccattacta ccacattcat ggcagtggca cgagcgaggc ggaaaacacg
     5821 ggtggcgtct ataacagtga ttgtagcaat cagataaggg accgaagtac cgaagattga
     5881 gagtatcaaa agggcaaaaa ttaatgaagg aatggacata agtacatcca caatacgact
     5941 taaaatctga tccacccaac caccaagtgc ggctgcaatc atgcctgtta gactgccaag
     6001 taaaaaagct aaaatcgtaa tgctcagtgc aaggccaatt gtattacggg caccgaaaat
     6061 catccgtgaa agcatatccc gacccaggtt gtctgtgcct aggagatact tgtctgacca
     6121 taattcaaaa gcccttccaa gtatttctct ttcaccgtac ggggcaatga agggtgcaaa
     6181 aattgccact atggaataga ttagaacaac cagcattcca aattttgcac tcaatggcgc
     6241 tgaggaaata attttgagta tattcgacat cagcgtggat gaagcagtct ggggtttgtg
     6301 acgatggaga tgatgtctgc tgagagattg agtagaatgt aaactgaagc aaagatcatt
     6361 gagcaggcct gtacaaccgg aatatcccgt ttggaaacac tgtcaaccat cagctgaccg
     6421 agtcccggat agacaaaaac aacttcgaca ataaccactc caactacaag ataagccaaa
     6481 tttattgcaa ccacatttac tattggagcc agtgcatttg gtagtgcgtg tcgcaggata
     6541 attttcatgg g
//
LOCUS       DTVD01000184            6529 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3632_length_6529_cov_355.099784, whole genome
            shotgun sequence.
ACCESSION   DTVD01000184 DTVD01000000
VERSION     DTVD01000184.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_6529_cov_355.099784"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="EYO68_06485"
     CDS             complement(<1..663)
                     /locus_tag="EYO68_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07486.1"
                     /translation="MKESHQSNPLLRKNVSLGLILDVCEGKVCRSKSKRAAQKETLRI
                     QKLGNKRRIFAEKGPNAPRCVIKRGGRQFVCSSCGIVSNESCRSFPSDGSTIFPGTNI
                     DSSDFAFTEGDTKETKCSKLKNPKFFKIETLINVESGEKGKVYDKVQSYYDKKKEVPI
                     MVNFFAEKVLRKVYRFFPKYYAKVGGKWISTVMRVRTTQGNEKKFIFETLTHIQKKNG
                     KLK"
     gene            662..1060
                     /locus_tag="EYO68_06490"
     CDS             662..1060
                     /locus_tag="EYO68_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07487.1"
                     /translation="MNDFIFSGVFNSLQLTALVLAGSPQIKKKIIKIQYCFMFFIVTP
                     FQKVALPASLRDLLILAESNFENKSKDKILYYAVNFTKFVASFTGKKLDLKRVIFEYK
                     CQLFLIFFAYLKLPVTKYSGQTKEEAEKKC"
     gene            1054..2532
                     /gene="guaB"
                     /locus_tag="EYO68_06495"
     CDS             1054..2532
                     /gene="guaB"
                     /locus_tag="EYO68_06495"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIC07488.1"
                     /translation="MLKDRIEDSLTFDDVLLLPGYSKVLPSEVSVKSRITQTLECNIP
                     LISAAMDTVTEHQTAICMAQEGGIGIIHKNMTAEDQATQVKMVKRSESGMISDPITVE
                     PEEPLQNALDLMEQYRISGVPVTKGRELVGILTNRDLRFETNFEQPVSALMTKGRERL
                     ITVATGIEMEEAKHLLHKHRIEKLLVVSEKFELVGLITIKDIEKARKFPDSNKDDLGR
                     LRVGAALGTSADLLSRAETLIKAGVDVVVLDTAHGHSQGVLDSILKLRSTYPELQIIG
                     GNIATGDAADALVKAGANAVKVGIGPGSICTTRMVAGIGVPQMTAIDKVSHALHKSDI
                     PVIADGGIKYSGDIVKAISAGAHTVMIGSLFAGTAESPGQVVLYQGRRYKIYRGMGSL
                     GAMKEGGKDRYFQSHIEEDVKFVPEGIEGRVPYKGHLSESIYQFVGGLRAGMGYTGTE
                     TIDELRSKPRFVKITSAGLRESHAHDIFITEEAPNYPMNVKS"
     gene            2568..3578
                     /locus_tag="EYO68_06500"
     CDS             2568..3578
                     /locus_tag="EYO68_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07489.1"
                     /translation="MFFVTSFIFGCSFHYDQGLQLEQEERWAEAAIEYRIALVENPED
                     TEIREALKRMNIHVAQENFEMYQQYLKQREYHKAYRRLEAALSQNPELVEARSEMRHW
                     WHLLITGKVDLEFNRFSSNLRLAEEMILQVRINTPNRKLLTGNISSETGIFFLEDVVY
                     RTQPKQLAEYTINSIGLKIKHKSSLGYVRNEFKKFINFRELFPLQVRGSIKNINLKTP
                     QNILDHRTSLLNEGENSTAWHPPRLVSYELQFDGDDIRVKSDMNHSEFAPSILYLNNS
                     DRRANIDFGVYQLQMNGSGRKWSIKRKTYRTSKDDYFYVLSSNISLNRYFYYDRVFRF
                     IQ"
     gene            complement(3698..4762)
                     /locus_tag="EYO68_06505"
     CDS             complement(3698..4762)
                     /locus_tag="EYO68_06505"
                     /inference="COORDINATES: protein motif:HMM:PF01571.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07490.1"
                     /translation="MEIYKIIDELHLNLGCRIQEFEGGAGLPTSYSDRLAEEINLLLH
                     GCGLFDLKACWLIALKGIDAGTFLQGMVTSDVLQLEVGEIQSSLICSNKGKIIHHLEI
                     FRSEKDEWIVICDPGEGQAVGTILDNFHIREDLELRLLNSGEMLRIDLSGPDAEKIAE
                     KLGYFRDKIKWTFEGELVLTAKFNLGKLPRLINLVYAGVLQKFIEQILQNEKADLIGL
                     EAFDEMRCLEGVPRSGVDYGKDNFPQEAGLGDHISFNKGCYIGQEPHARMYHRGHPNW
                     ISVWLSVPENSKATSGALLFHAGKEIGKITSLGSFSKDSVFRGIAMIRHEAAKEKSLL
                     SLSVDEPAEIVHEPLPSIIV"
     gene            4851..5741
                     /gene="folD"
                     /locus_tag="EYO68_06510"
     CDS             4851..5741
                     /gene="folD"
                     /locus_tag="EYO68_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIC07491.1"
                     /translation="MSELISGKIISAQINEELCLEVKQLKTKGIEPCLAVVLVGEDPA
                     SEVYVRNKKRTSEEIGMRSIGHNLPDTTTQRELETLIQSLNADPAVHGILCQFPLPEG
                     LDEKKVIQTIAPEKDVDGLHPLNAGLIAMGIPKFISCTPYGVLQMLKRSGISTSGKNA
                     VVLGRSNLVGRPIATLLSSKGWDATVTVCHSRTSDLAEVTSQADILIAAIGIPEFVKA
                     NMVKPGAVVIDVGINRIDDPNKAKGTRLVGDVAFEEVAAKASFITPVPGGVGPMTIAM
                     LMVNTVNAARWQNGLTEMDL"
     gene            6027..>6529
                     /locus_tag="EYO68_06515"
     CDS             6027..>6529
                     /locus_tag="EYO68_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07492.1"
                     /translation="MKKLLSTILLAGVVLWSASQAMAYKPAVVFDMGGKFDKSFNEGI
                     WNGLEKFRKETGIKYREFEVQQEAQREQFLRKLAKRGSDPIIAVGFGQAAALSKVAKE
                     FPNTRFSIIDMVVDLPNVQSIIFKEHEGSFLVGVLAALKSETGKVGFVGGMDIPLIHK
                     FACGYVQG"
BASE COUNT         1971 a         1304 c         1336 g         1918 t
ORIGIN      
        1 tttcagtttt ccattctttt tttgaatatg agtcagggtt tcaaaaatga attttttctc
       61 gtttccctga gttgtacgga cacgcatcac tgttgaaatc catttgcctc caaccttggc
      121 ataatattta ggaaaaaacc gataaacttt tcgaaggact ttttcagcaa aaaagttaac
      181 catgataggc acctcctttt tcttgtcata ataactctgc accttgtcat atactttgcc
      241 tttttccccg gattcaacat ttattaatgt ttcaattttg aaaaattttg gatttttcaa
      301 tttagaacat tttgtttcct tcgtgtctcc ttcagtaaag gcaaaatccg acgaatcaat
      361 atttgttccg ggaaaaatag tactcccatc tgatgggaaa ctgcggcaac tctcattgga
      421 aacaatcccg cagcttgagc agacaaattg tcgtccacca cgcttgataa cacaacgtgg
      481 tgcattgggt cctttctccg caaatattcg ccgcttgttt ccaagctttt gtatacggag
      541 tgtttctttt tgtgctgcac gcttactttt tgacctgcat accttaccct cacaaacatc
      601 taaaatcagc cctaaactaa cattcttcct taacagtgga tttgactgat gtgattcttt
      661 catgaatgat tttatctttt ccggagtatt caattcctta caactgactg cacttgtact
      721 tgctggcagc ccgcaaatca aaaagaaaat aattaaaata caatattgtt ttatgttctt
      781 catagtcact ccattccaaa aagtagcatt acctgcctct ttaagagatt tgctgatact
      841 agctgaatcc aattttgaga acaagtcaaa agacaaaatt ttatattatg ctgtaaattt
      901 tacgaaattt gttgcttctt tcaccggcaa aaaacttgac ttaaagcgtg taatatttga
      961 atataagtgt caattatttt taatcttttt tgcatattta aaattgcctg ttaccaaata
     1021 ttcaggacag acgaaagagg aagctgagaa aaaatgttaa aagaccgcat tgaggacagc
     1081 ctcacttttg atgatgtact tctcctcccg ggatattcca aagtattgcc ttcagaagtt
     1141 tcagtaaaaa gcagaatcac ccaaactctt gaatgcaata taccgttaat cagtgcagca
     1201 atggacacgg ttacagaaca ccagacagca atctgcatgg ctcaggaagg tggaattgga
     1261 attattcaca aaaacatgac tgcagaagat caggcgactc aggtgaagat ggtcaagcgt
     1321 tctgaaagcg gtatgatttc agatcctata actgttgaac cggaagagcc gctgcaaaat
     1381 gcgctggact taatggaaca ataccggatt tccggagttc ctgtcactaa gggtcgtgaa
     1441 cttgttggta ttttgacaaa ccgcgattta cgttttgaaa caaattttga gcaaccagtc
     1501 tcagctttga tgactaaagg acgggaacga ttaataacgg tagctaccgg cattgaaatg
     1561 gaggaagcca aacacctgct tcataaacat cgtattgaaa aactgctggt tgtttcagaa
     1621 aaatttgaac ttgttggtct gattaccatc aaggatattg aaaaggcccg taagttccct
     1681 gattccaata aagatgacct gggtcgcctc agggtaggtg ctgctttagg aacatctgcg
     1741 gatttattat cccgtgcaga aacactgatt aaagctggtg ttgatgtagt tgtgcttgat
     1801 actgcacatg gccattcaca aggtgtgtta gacagtattt taaaattgcg ctctacttat
     1861 cctgaactgc aaataattgg aggaaatatt gcaaccggcg atgcagctga tgctttggtt
     1921 aaggctggtg caaatgcagt taaagttgga attggaccgg gatctatctg tacaacaaga
     1981 atggtggcag gtatcggagt cccacaaatg acggctatcg ataaggtttc acatgcactt
     2041 cataagtcag atattcctgt tatagcagac ggtggcatca aatattcagg tgacatcgtt
     2101 aaagcaattt ctgccggagc acacacggtg atgatcggca gcctatttgc cggaacagca
     2161 gaatcacctg gacaagtagt tctttatcaa ggcaggcgct acaaaatata ccgtggtatg
     2221 ggttcattgg gtgcaatgaa agagggcggc aaagatcgct attttcagag tcacatagaa
     2281 gaggacgtaa aattcgttcc tgaaggcatc gagggtcgtg tgccgtacaa ggggcacctc
     2341 tcagaatcta tctatcagtt tgttggcggg ttgcgtgccg gaatgggata taccggtact
     2401 gaaactattg atgaattacg atcaaagccc cggtttgtaa aaattacttc agccggctta
     2461 cgggaaagcc atgctcacga tatcttcatt accgaagaag ccccgaatta tccaatgaac
     2521 gttaaatcat gagcttctgc cggttaccta tcctaaacaa actcatcttg ttttttgtta
     2581 cttccttcat tttcggttgt tcctttcatt atgatcaggg actgcagtta gaacaggaag
     2641 aacgctgggc agaagctgcg attgagtacc gtattgcctt agtcgaaaat ccggaagaca
     2701 cggaaataag ggaagctttg aaaaggatga acatccatgt tgcacaagaa aattttgaaa
     2761 tgtatcagca gtatcttaag cagcgtgaat atcacaaagc ttaccgcaga ttggaggctg
     2821 ccctgtctca aaatccggaa cttgttgaag cgcgttcaga aatgcggcat tggtggcatt
     2881 tattgataac aggtaaagtt gatttggaat ttaaccgctt ttcttccaat ctgcgactgg
     2941 cggaagaaat gatcctgcag gtccggatta ataccccaaa cagaaaatta ttaaccggaa
     3001 acatttcaag tgaaactgga attttctttc ttgaggatgt ggtatatcgt actcagccaa
     3061 aacagcttgc cgaatacact atcaactcaa ttggcctgaa aattaaacat aaatcttctc
     3121 ttggctacgt ccgtaatgaa tttaaaaagt ttattaattt cagggaacta tttcctttac
     3181 aagtcagggg atcaattaaa aacataaatt tgaaaactcc gcagaatatt ctggatcacc
     3241 ggacttcctt acttaatgaa ggagaaaatt caacagcatg gcatcctccc cgtctggtca
     3301 gttatgaact tcagtttgac ggtgatgata ttcgggtgaa atcggatatg aatcatagtg
     3361 agtttgctcc ttctatactt tacctgaata actcagaccg gcgggcaaac attgattttg
     3421 gagtttatca gcttcaaatg aacggttcgg gacgaaaatg gagtatcaaa cgaaaaacat
     3481 accgcacctc aaaagacgat tatttttacg tactgtccag caatatatca ctcaaccgtt
     3541 acttttatta cgatagggtt tttcggttca ttcaataact gatcagaact gaaagtagaa
     3601 aagaactgtc actgaaaata cgagcataat acccgtgaag cagcccattg ggaccggaag
     3661 taacggaagg ggaatgtgcc gtagcatggt gatgatatta aacaattatt gatggtagtg
     3721 gttcgtgtac aatttcagct ggttcatcaa ctgacaaact aagcagagat ttttcttttg
     3781 ccgcttcatg gcgaatcatt gcaattccac ggaaaacact gtctttagag aaacttccca
     3841 aactggttat ttttccaatt tcttttccgg catgaaaaag cagagctccg gaagttgctt
     3901 tggaattttc cggaacactt agccagactg agatccagtt tggatgccct cgatgataca
     3961 ttctggcatg aggttcctgt cctatgtaac agcctttgtt gaaggaaatg tggtccccaa
     4021 gacctgcttc ttgagggaaa ttgtctttgc cgtaatcaac tcctgaacgt ggtacaccct
     4081 ctaaacaccg catttcatca aatgcttcaa gaccaatcaa atccgctttc tcattttgca
     4141 atatctgttc tataaatttt tgcaaaacac ctgcgtaaac aaggtttatt aaacgtggca
     4201 gttttcccaa attaaatttt gctgttaaaa ccaactctcc ttcaaatgtc cattttattt
     4261 tatctcgaaa atatcccagt ttttctgcaa ttttttctgc atccggacca ctcaagtcaa
     4321 ttctcagcat ttctcccgaa ttcagcagac gtaattccag atcctctcgt atgtgaaaat
     4381 tatctaaaat cgttcctact gcttgccctt caccaggatc acaaattaca atccattcat
     4441 ctttttcact gcgaaaaatt tccagatgat gaataatttt acccttgttg ctgcaaatca
     4501 aacttgactg aatttctcca acttccagct gcaatacatc gcttgtaacc attccctgta
     4561 aaaatgtacc tgcatcaatg cccttcaaag caatcagcca gcaggcttta aggtcaaata
     4621 atccacaacc atgcaggagc aggttgattt cttctgccaa tctgtcagaa taactggtcg
     4681 gcaaaccagc gcctccttca aattcctgaa tgcggcatcc taaattaagg tgaagttcgt
     4741 caattatttt gtagatttcc ataaattgta tcagaatatt ttggctttaa ttatacagga
     4801 attgtggaaa ttttgtaacc cccattaaac tataatgaaa aggaaaatac gtgagtgagt
     4861 taatttctgg aaaaataata agtgcgcaaa tcaacgaaga attgtgcctt gaagtgaaac
     4921 agttaaaaac aaaaggtata gagccgtgtc tggctgtagt tctggttgga gaagatcctg
     4981 cttcagaagt ctatgtgcgt aataaaaaac ggactagcga agaaatcgga atgaggtcca
     5041 ttggacacaa ccttccggac acaactactc agcgagagtt agagacatta atacaaagtc
     5101 ttaatgcaga tcctgcagtt catggaattt tgtgccagtt tcctctgcct gaaggtcttg
     5161 atgaaaaaaa ggttattcag acaattgctc cggaaaaaga tgtggatggt ttacatcctc
     5221 ttaatgcagg actgattgct atgggcatac ctaagtttat ttcctgtacg ccatacggtg
     5281 tcttgcaaat gctaaaaagg agtggaattt caacttcagg gaaaaatgct gttgtgcttg
     5341 gcagaagcaa tcttgtaggc cgtccgattg ccactcttct ttccagtaag ggttgggacg
     5401 caacagtgac tgtttgtcac tcaagaacca gtgatttggc tgaagttacc tctcaggcag
     5461 atattttgat tgcggcaatc ggaataccgg aatttgttaa agcgaatatg gtcaaacccg
     5521 gagcagtcgt aattgatgtc ggcattaacc gcattgatga cccaaacaaa gctaaaggaa
     5581 cccgcctggt cggagatgtt gcatttgaag aagttgcagc aaaagcctca ttcatcacac
     5641 ccgttccagg cggagtcggt ccaatgacta tcgccatgct gatggtaaac acagttaatg
     5701 ccgcacgctg gcaaaacgga ttaactgaaa tggatttata aaaatcaatc tttgctgtat
     5761 ttttcagcca ttaaacctgt tctactaata aaaacagaaa attgagagaa ggtaactatt
     5821 atcctaaaaa gaaatatatt tgaatttttt tgcttttaac aaagatttcg attgaatttt
     5881 caaaaaaaac acaatatata taatgtgtcg tacactatag tttagtgacg ttagggggat
     5941 tgtccctctt cctccaggtt tgtacaggga ggattttcaa taatctgatc cggacagagt
     6001 ttcggatttt acataaggag tatgttatga aaaaattgct ttccaccatc ctgctggctg
     6061 gtgtagtact gtggagcgct tcacaggcaa tggcttacaa accggctgtg gtttttgaca
     6121 tgggtggtaa atttgacaag tcattcaacg aaggcatttg gaatggactt gagaagttcc
     6181 gcaaagaaac aggcatcaag taccgagaat ttgaggtaca gcaggaagct cagcgtgagc
     6241 agttcctgcg taaacttgca aaacgtggtt ccgacccgat tattgcagtt ggttttgggc
     6301 aagctgcagc actgtcaaaa gttgctaaag aattcccaaa tacaagattc agtatcattg
     6361 acatggtagt agacctgcca aatgtacagt ctatcatctt taaggaacat gaaggttcat
     6421 tcctggtagg tgttttggct gcattgaaaa gtgaaacagg aaaagtcggt tttgttggcg
     6481 gaatggatat tcccttaatc cacaaatttg cttgcggata tgtacaggg
//
LOCUS       DTVD01000185            1583 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_37007_length_1583_cov_457.994764, whole genome
            shotgun sequence.
ACCESSION   DTVD01000185 DTVD01000000
VERSION     DTVD01000185.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37007_length_1583_cov_457.994764"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(103..1146)
                     /locus_tag="EYO68_06520"
     CDS             complement(103..1146)
                     /locus_tag="EYO68_06520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIC07493.1"
                     /translation="MKRTENLNLVDISPLTSPKNLKELLPVSKTAAETVIQARNSIKS
                     ILTGEDQRLLVIVGPCSIHDPEAAHDYALRLKKLAAEVSDTILIVMRVYFEKPRTTVG
                     WKGLINDPELNGSHKVNEGIELARRILLEINNLGLPCANETLDPITPQYMADLISWSA
                     IGARTTESQTHREMASGLSMPVGFKNGTDGGLNVALNAMKSALQQHHFLGINNDGVSS
                     VIQTSGNQNVHLVLRGGNNGPNYDAVSVHIAADALASGGLPKAIMIDCNHANSGKDPS
                     RQELVLRNTILQIKDGDNSIIGTMIESNINGGNQPISAEMKYGVSVTDACLDWDNTRR
                     ILLDAHQSLKNGS"
BASE COUNT          437 a          335 c          323 g          488 t
ORIGIN      
        1 gtgtccaatt caaaattcgt tctattgtcc attgctcaat agtcacatct tttgccgata
       61 attttattta tagtgatttt agtctgcaat agcaataaaa tttcagctgc cgtttttcag
      121 agattggtgt gcatccagta atattctccg agtgttgtcc caatccaggc aggcatctgt
      181 tacagaaaca ccatacttca tttctgctga aattggctgg ttcccgccgt taatgttact
      241 ttcgatcatt gttccaatga tggaattgtc gccgtcttta atttgaagaa ttgtgtttct
      301 cagtaccagt tcctgccggg aaggatcttt tccggaattt gcatgattgc agtcaatcat
      361 gatggctttg ggcaagcctc ctgaagcaag tgcatctgca gcaatgtgga ctgaaactgc
      421 atcgtaattt ggtccgttgt ttccgccacg taaaaccagg tgaacatttt gattacctga
      481 agtttggatg acagaggaaa caccatcatt attgattccg agaaaatgat gttgctgcaa
      541 agcagatttc attgcattga gagccacatt caagccaccg tcagttccat ttttaaaacc
      601 gaccggcatc gacaaaccac ttgccatttc acggtgtgtc tgagattcag ttgtacgtgc
      661 tccgattgcg ctccaggaaa tcaaatctgc catatattgt ggtgtgatag gatcaagagt
      721 ttcattagcg cacggcaggc cgagattgtt gatttccaac aaaatcctac gcgcaagctc
      781 tattccttca tttactttgt ggcttccgtt aagttccgga tcattgatca agcctttcca
      841 gccgaccgtc gtccttggtt tttcaaaata gacacgcatc acaatcagaa ttgtgtcaga
      901 aacttcagca gcaagtttct tcagacgtaa ggcatagtca tgtgctgcct ccggatcatg
      961 aatagagcag ggaccaacaa taaccagtaa acgctggtct tcaccagtca gaatactttt
     1021 tatcgaattt ctagcttgaa ttacagtttc tgctgcagtc tttgaaaccg gtaaaagttc
     1081 tttaagattt tttggtgaag ttaatggtga aatgtcaaca agattcaggt tttcggtacg
     1141 tttcattatt tactgttcca attcagaatt gaaaatattt tgtattataa aattaagttt
     1201 cactgaaaaa gtatttcttt tgtcattcct gctccacgca gcacttcgcc cttaatgaca
     1261 atggagaaga atctaaagca gcgtcacatc ccagtgaaaa cagtttgcat gatgaggtac
     1321 tcaagcaacg gatgtgcatg tcttttatgt gggttcagga gaaaccattg cacatgacat
     1381 tcaatatcga ataattacat attgatttta ttgaataata tcactaatcc gcaagcccta
     1441 attacgacag gcagcgtaat aacagaaaac cctcagtttg ttttaaccaa attagacggg
     1501 atttatgttg aagtatgcat caggcaggtg gtcggagcga ctggatttga accagcgacc
     1561 gcacgccccc caggcgtgtg cgc
//
LOCUS       DTVD01000186            6455 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3708_length_6455_cov_156.397969, whole genome
            shotgun sequence.
ACCESSION   DTVD01000186 DTVD01000000
VERSION     DTVD01000186.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3708_length_6455_cov_156.397969"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /gene="infB"
                     /locus_tag="EYO68_06525"
     CDS             <1..2188
                     /gene="infB"
                     /locus_tag="EYO68_06525"
                     /inference="COORDINATES: protein motif:HMM:PRK05306.1-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIC07494.1"
                     /translation="SKISTLESQETLPESGDPAPENKVEPDADTVEDSKQSKLTPLER
                     DPDKTVLPDDEKSAKLEKKSKKTKIFFKHPQTDDEEFSSKNRGSRPSEFENKRRGGGG
                     QRDYNYLRERRPRRRRKDFPKNRLDRKISSKVSDTNAPKHVFNPRKKSIRIGNQVGVA
                     ELAGLIGIKVPEILKKLMSLGIMATINQTITGETAELIAADFDITIEVESFELSDLLR
                     EEELDVSELEERAPIVTIMGHVDHGKTSLLDKIRESRIASGEAGGITQHIGAYHLEVK
                     GNQVTFLDTPGHEAFTSMRARGANITDIVVLVVASDDKVQPQTVEAIQHARAANVPII
                     VAVNKIDRPESDPIRIQQELLSHELIPEDLGGSTIFVKVSAKTGDGIDELLEMIHLQA
                     EILELKSTSKGFARGTIIESRVRKGQGPVGTVLVQRGTLKIGDYFVMGSIHGRIRAMF
                     NEVGDTMIEAGPAIPVEISGLSNVPEVGVNFVVLEDEKIARQIAEDLEQKLRSETIRE
                     YQKTSLDNLFSKIDEGKVTELRLILKGDVQGSVEALKTSLQQIGDERISLKFLLCSVG
                     NVTETDITLASASDAIIVGFNVQMDAKARETRSSEGVDVRLYDVIYNALDDVKAAMEG
                     LLEPEIREEVLGHLEVRQVFSSGKNGIVVGGLVTDGKLLRNSLIRVLRKKESIFEGSI
                     ISLKRFKDDVQEVAQNYECGIVLNFLEIENGDIVEAYEQIEESARL"
     gene            2195..2578
                     /gene="rbfA"
                     /locus_tag="EYO68_06530"
     CDS             2195..2578
                     /gene="rbfA"
                     /locus_tag="EYO68_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIC07495.1"
                     /translation="MHKTSRSNFSTLVHRRLSEVLLFQSSDPRFSKVTISRVETTPSL
                     TSAKVYVSIFPTKGKEELIASLNHASGFFSRKLGKVLKTRNTPHLTFEYDRGFDHSDE
                     IEILLRNVLPEDTGQQRESEFDKNE"
     gene            2591..3559
                     /gene="truB"
                     /locus_tag="EYO68_06535"
     CDS             2591..3559
                     /gene="truB"
                     /locus_tag="EYO68_06535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIC07496.1"
                     /translation="MLFDIRIKIKNYLRIININKTQGWTSFDVVRFIKRKFNEKKVGH
                     LGTLDPMATGVLPVFLGQATRLIPLFNDADKTYRAVCKLGESTDTFDAEGRVTERHNL
                     GNLNPEQVTKAVHTFLGPQKQQNPAFSASKINGIPAYKLARQGLKVPAKTRSVIFHEL
                     DVEAVDLPFVQIRIHCSKGTYIRALANDLGQKLKVGAHLTSLDRLACGKWFKSENSIS
                     IEKLQETVTENNVPWISPLELLDHLNTIVGSAEMVVSIKQGRRVEVLQQNWTESGVKM
                     TENCVIESETNSVKTKVVDSCQNLVAIGHIMWENEASYFHPIKVFV"
     gene            3578..3847
                     /gene="rpsO"
                     /locus_tag="EYO68_06540"
     CDS             3578..3847
                     /gene="rpsO"
                     /locus_tag="EYO68_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIC07497.1"
                     /translation="MLTKNQKVKIVNKFGKNNADTGSTEVQVALLSGRINSLTDHLRS
                     QPKDFSSRRGLLKLVGQRRNFMNYLKNRNSERYAAITKQLEIRVK"
     gene            4179..6251
                     /gene="pnp"
                     /locus_tag="EYO68_06545"
     CDS             4179..6251
                     /gene="pnp"
                     /locus_tag="EYO68_06545"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIC07498.1"
                     /translation="MKIVEREFGPATMKLETGRMAKQANGSVLVTYGDTVVLVAATAA
                     KGSGTGADFFPLSVFYVEKGYAAGRIPGGFLKREARLSDSETLISRIIDRPLRPAFED
                     NYLAETMIAATVLSHDLIHPSDVAAMNGASAALAISDIPFHNPLGGVRVGRIDGKFVT
                     NPSPEQLEVSELNIFMAASKDAILMVEGEAVGVSEEIMLDALWYGQEAVLPIIEMQEE
                     LMKSVGKKKRTVDVPVVDSELKSKVEAITPKRLNEALRIKDKIERYERLDTLKDEIKN
                     EILGDSPDADSAKELSQIFADIKKDEMRTRLIKDQIRIDGRKPTEIRPITCETKVLPR
                     THGSALFTRGETQALAVTTLGAREDEKMIESLDGVSFKRFTLHYNFPSFSVGEVRPPR
                     GPGRREIGHGTLAERGLTPVIPTKAEFPYTIRLVSEILESNGSSSMATVCAGSLAMMD
                     AGVPLKEATAGIAMGLISEGKNNVILSDILGDEDHLGDMDFKVIGTENGITALQMDIK
                     IKGLGQKVVEQALMQAREGRLHILGKMAEALKESRSGLSTYAPRFITHKIAQDKIGTV
                     IGPGGKMIKSIVEKTGVKINIDDDGIVSIMSRDHQAVDDALEIVQNLTRTIEIGETYT
                     GPVKKVMDFGAFVEVFPGTEGLVHISNLAEGRVEKVTDVCKEGDIVTVKATGVDRRGK
                     IQLSIKDV"
BASE COUNT         2111 a         1180 c         1421 g         1743 t
ORIGIN      
        1 ctcaaaaata agcacattgg aatcccagga aactttacct gaatctggag atcctgctcc
       61 agaaaacaaa gttgaacctg atgctgacac agttgaggac tcaaaacaat ctaagcttac
      121 tccgctggaa agagatcctg ataagacagt tttacctgat gatgaaaagt ctgcaaaatt
      181 agaaaaaaaa tcaaagaaaa ctaaaatatt tttcaaacac cctcaaactg atgatgaaga
      241 attctcatca aaaaacagag gttcccgacc ctcggaattt gagaacaaac gaaggggagg
      301 tggtggacag agagattata attatcttcg tgaaagaaga cctcgccgcc gcagaaaaga
      361 ctttccaaaa aatcgattgg ataggaaaat ttccagcaaa gtttctgata ctaatgcacc
      421 aaagcacgta ttcaatccac gtaaaaaaag catccgaata ggaaatcagg ttggtgttgc
      481 cgaactggca ggcttaattg gtataaaagt tccagaaatc ttaaaaaaac tgatgtcctt
      541 aggcatcatg gcaactatta accagacaat cacaggagaa actgcagaac ttattgcagc
      601 agattttgat attacaatcg aagtcgaatc gtttgagttg tctgacctcc tcagagaaga
      661 agaacttgat gtttcagagt tagaggaacg agcaccgatt gttacaatta tgggacatgt
      721 tgatcacgga aaaacatccc ttcttgataa aattcgcgaa tctcgaatcg cctctggaga
      781 agctggaggc ataacacaac atattggagc atatcatctt gaagtaaaag gcaatcaggt
      841 cacttttttg gatacacccg gacacgaagc tttcacatca atgagagccc ggggggcaaa
      901 tatcactgat atagtggtac ttgttgttgc gtcagatgat aaggtgcagc ctcaaacagt
      961 tgaagctatt cagcatgcca gagcagcgaa tgttccgatt attgttgcag taaataaaat
     1021 tgatcgtccc gaatctgacc caataagaat tcaacaggaa ttactctcac atgaattaat
     1081 tccagaagat ttgggtggga gcactatttt tgtaaaagtg tcagcaaaaa caggtgacgg
     1141 cattgatgaa ctactggaaa tgattcattt acaggcagaa atacttgaac taaaatcgac
     1201 ctcaaaaggt tttgctcgtg gaacaatcat tgagtcacga gtcagaaaag gtcaggggcc
     1261 agtaggtact gttcttgttc aacgcggaac ccttaaaatt ggagattatt tcgtcatggg
     1321 cagcattcat ggacgaatac gggctatgtt caatgaagtt ggtgatacaa tgatagaagc
     1381 tggtcctgca attcctgttg aaatttccgg tttaagcaat gtacccgaag taggagtaaa
     1441 ttttgttgtt cttgaagatg aaaaaatagc acggcaaatt gctgaggatc ttgaacaaaa
     1501 acttcgatct gaaaccatcc gggaatacca aaaaaccagt ttggacaatc ttttcagtaa
     1561 aattgatgaa ggtaaggtta ctgaactccg attgatttta aaaggagatg tacaaggttc
     1621 cgttgaagct ctcaaaactt ctctgcaaca aatcggtgat gaacggattt ctctaaagtt
     1681 cctcctttgc tcagtaggta acgttactga gaccgacata acactagctt ctgcttccga
     1741 tgcaattatt gttggcttta atgtgcaaat ggacgcaaaa gccagagaaa cgcgatcaag
     1801 tgaaggtgta gatgtgaggc tttatgatgt tatatacaac gcattggatg atgtaaaagc
     1861 agctatggaa ggtctcctgg aaccagaaat tagagaagaa gtattaggtc atttagaagt
     1921 tcgtcaggtt ttttcttctg gaaaaaatgg cattgtagtc ggaggtttgg ttacagatgg
     1981 gaagcttctc cgcaatagcc ttatacgagt attacgtaag aaagaaagta tctttgaggg
     2041 ttcaatcatt tctctgaagc gcttcaaaga cgatgttcag gaagttgcac aaaattatga
     2101 gtgcggaata gtactgaatt ttctggaaat tgagaatggc gatattgtag aagcttacga
     2161 gcaaattgaa gaaagtgcac ggctgtaatt gattatgcat aaaacaagta gaagcaattt
     2221 cagcactttg gtgcatcgtc gtttatctga agttcttctt tttcaaagca gtgacccgcg
     2281 tttcagcaaa gttacaatca gcagagttga aacaactcca agtttgactt cagcaaaagt
     2341 ctatgtttca atttttccaa ccaaaggcaa agaagaattg attgcatctt taaatcatgc
     2401 atcaggattt tttagccgaa aattgggaaa agtcctcaaa actagaaaca caccacatct
     2461 gacttttgag tacgacagag gatttgacca cagtgatgaa attgaaatat tgttgcgtaa
     2521 cgttcttccg gaagatactg gacagcagcg agaatccgaa tttgacaaaa atgaatagag
     2581 tccggttaat atcctttttg atattcgaat aaaaataaag aattacttgc ggataatcaa
     2641 catcaataag actcagggat ggacttcttt tgatgtcgtt cgtttcatta aacggaagtt
     2701 taatgaaaaa aaagtcgggc atctcggcac tttagatccc atggcaacag gagttttacc
     2761 tgtatttttg ggacaggcaa cgcgcctgat tcctttgttc aatgatgctg acaaaactta
     2821 ccgtgcagta tgtaaattag gtgaatccac tgacacattt gacgcagaag gtagggtcac
     2881 tgagcgtcat aatttaggta atttaaatcc ggaacaggtt acaaaagcgg ttcacacatt
     2941 tctaggacca cagaagcagc aaaatcctgc attttctgcg tcaaaaatca atggtattcc
     3001 tgcctataaa cttgcaagac aaggattgaa agttcctgct aaaaccaggt cggtaatatt
     3061 ccatgaatta gacgtagaag ctgtagatct gcccttcgtt caaatccgga tacactgttc
     3121 taaaggcaca tacatccggg cattggccaa tgatcttgga cagaagctga aagttggagc
     3181 acatctaact tctctggacc gtttagcttg tggaaagtgg tttaaaagtg aaaactctat
     3241 atcaatagaa aaactacaag aaacagtaac cgaaaataat gtgccgtgga tttctccatt
     3301 agagttgctg gatcacctta acacaatcgt aggttctgct gaaatggttg tttccatcaa
     3361 acagggacgt cgtgttgaag ttttgcaaca aaactggact gaaagtggcg taaaaatgac
     3421 tgaaaattgt gtaatagagt cagagacaaa ttcagttaaa accaaagttg ttgattcttg
     3481 ccaaaattta gttgctatcg gccacattat gtgggagaat gaggcaagtt attttcatcc
     3541 aataaaggta tttgtataga aaacaaaata atcttatatg ctgacaaaaa atcaaaaagt
     3601 caaaattgta aacaagttcg gaaaaaataa tgctgatacc ggatctacag aagtacaggt
     3661 agcattactt tccgggcgaa ttaattcatt gacagaccat ttacgaagcc aaccaaagga
     3721 ctttagttca cgacgtggtt tgttgaaact agttggtcaa cgacgaaatt tcatgaatta
     3781 tcttaagaat agaaatagcg aaaggtatgc cgctattact aaacaactag agattcgagt
     3841 caagtgactc atacaaaact tcacagaaga cttgtccacg ggggctggtc ttctgccagg
     3901 tataatttgg caaaagctga attctaccat tgcaattccc ccgtatcatg tagcatcaag
     3961 tctgctttcc ttctttcagt actttttttc agcttgttga aaattgattt atgaatattg
     4021 cttttagcac tgtccagaac tggaagatta tttttttcca agtcaggaaa gaagaaagaa
     4081 tttgtatgct gacatttaaa gaataccgga attcagacat taataacagg cagtgccgtt
     4141 aagttagaac cggtagaacg ttgataatta ggagtagaat gaagatagta gaaagagaat
     4201 ttggaccagc aactatgaag ctggaaaccg gaagaatggc caaacaggcc aacggatccg
     4261 tattagtaac atacggtgac acagtagtac ttgtagctgc aactgcagca aaaggatcag
     4321 ggacaggagc agattttttc ccactttcag ttttttatgt tgaaaaagga tatgcagccg
     4381 gacggattcc tggaggtttc ttaaaacgtg aagctcgact ttcagatagc gaaactctga
     4441 tttcacgcat tatagatcgt ccgctgcgtc cagcatttga agacaattac ctggcagaaa
     4501 caatgattgc ggcaaccgtt ttaagtcacg acctgataca tccatctgat gttgctgcta
     4561 tgaacggcgc ttctgcggcg ttggctattt cggatatccc ttttcacaat cctcttggag
     4621 gtgtcagagt tgggagaatt gatggtaaat ttgtaaccaa tcctagtccg gaacaacttg
     4681 aagtaagtga acttaacatc tttatggctg catccaaaga tgcaatttta atggtggagg
     4741 gtgaagctgt tggtgtgagt gaagaaatta tgctggatgc actttggtat ggtcaggaag
     4801 cagttctgcc aatcattgaa atgcaggaag agctgatgaa gagtgttggc aagaaaaaac
     4861 gtactgtaga tgtgccggtt gtagattcag aattaaaatc aaaagttgag gcaattacac
     4921 caaaacgact taatgaagct ttaaggatta aagataaaat agagcggtac gaacgtctgg
     4981 acacattaaa agatgaaata aaaaacgaaa ttcttggaga ttcacctgat gcagattctg
     5041 caaaagaact aagtcaaata tttgctgaca tcaaaaaaga tgaaatgcgc acacgtttga
     5101 ttaaagatca aatccggata gacggacgaa aacctactga aatcagacca atcacctgcg
     5161 aaactaaagt tttgcctcga acacacggtt ctgccttgtt cacacgcggt gaaacacagg
     5221 ctcttgctgt taccactctt ggagcgcgag aagatgaaaa aatgattgaa tcactggatg
     5281 gtgtctcttt taaaagattc acgctgcact acaactttcc cagttttagt gtcggtgaag
     5341 tacgtcctcc tcggggaccc ggacgaagag agattggaca cggaaccctg gctgaacgcg
     5401 gattaactcc ggttattcca acaaaagctg aatttcctta tacaattcgt cttgtttctg
     5461 aaattcttga atcaaatggt tcgtcttcaa tggctactgt ctgtgctggt tctctggcca
     5521 tgatggatgc cggtgttcct ctcaaggaag caaccgctgg tattgccatg gggctcatca
     5581 gtgaaggtaa aaacaacgtt atactttcag atattcttgg tgacgaagat catctgggtg
     5641 acatggattt taaagttatt ggtacagaaa acggaattac cgccctgcaa atggatatta
     5701 aaatcaaggg acttggacag aaagtagttg aacaagctct gatgcaggca cgcgaaggcc
     5761 gactgcacat tttggggaaa atggcagaag cactgaaaga atcccgtagc ggtctatcta
     5821 cttatgcgcc ccgctttatc actcataaaa ttgcacagga taaaatcggt acggtcattg
     5881 gacctggcgg taaaatgatt aagagtattg ttgagaaaac cggtgtaaag attaacatcg
     5941 atgatgatgg tattgtttcc atcatgtcac gtgaccatca agcggtggat gatgctctgg
     6001 aaattgtgca aaacctgact cgaacaattg agattggaga aacttatacc ggacctgtca
     6061 agaaggtgat ggactttggc gcatttgtcg aagttttccc tggaacagaa gggcttgtac
     6121 acatttcaaa tctggcagaa ggtagagtcg aaaaagttac agatgtttgc aaagagggtg
     6181 atattgtcac cgttaaagca accggtgttg acagacgagg taaaattcag ctcagtatca
     6241 aagacgtcta attcagagaa ttcaccacaa accccgtata taaaattgtc tgctcatcct
     6301 gtttcaaaaa caggagggca ggcatcccac ctgaaacttt attctgtccc attagttgct
     6361 ctactgttac tagttttatt gtgtcagcca gaacgcctgt gagggatctg attatatagc
     6421 agtatttatt agatctcatt ctttggtcga gatta
//
LOCUS       DTVD01000187            1573 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_37377_length_1573_cov_324.303689, whole genome
            shotgun sequence.
ACCESSION   DTVD01000187 DTVD01000000
VERSION     DTVD01000187.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37377_length_1573_cov_324.303689"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(298..675)
                     /locus_tag="EYO68_06550"
     CDS             complement(298..675)
                     /locus_tag="EYO68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07499.1"
                     /translation="MSENNTLIPEDLGSEREKEIGQHIGYRYDVNLVPDYERLTPFLK
                     KYLEVMNWQDLNWLEDVHMGYEEDRPAVFDRNINGWVTVPKEMDLPDNQQDRDMIARE
                     LLIKFQMSKRHPMVILKDNYGKF"
     gene            complement(782..>1573)
                     /locus_tag="EYO68_06555"
     CDS             complement(782..>1573)
                     /locus_tag="EYO68_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIC07500.1"
                     /translation="VAEMRSLPAGIDQRSPARHPDWLGPDDLSLKINEIREATDAQIP
                     IQLKLGSARVYDDVRMALKTNPDSIYIDGMEGSTGAGPHLATEETGVPGIAAIRQARK
                     AFDDLGYTGKISLVYAGGIRNGADVAKAIALGADAVAIGHSAMMALNCNKDIPEADYE
                     KEMGVEAGYCYHCHTGRCPVGVATQDPELRKRLDPDKAAERVYNFLHCLTIECQMFAR
                     ACGKTNVHSLEPEDLAALTMEASALAQVPLAGSQHTVGRPDMNRY"
BASE COUNT          394 a          388 c          343 g          448 t
ORIGIN      
        1 ttcaatccgc acctctctcc ggatgtaatc gttctaggtt ttgaaccgga ttggtctgac
       61 tccagtgcag accccacttc gttttaagga gaacacttcg agaaaaaatc aactaggggc
      121 ttgctgaaaa gtatttcttt tatcatccag gacttgatcc gggatccaga ggttagataa
      181 ggttgaggaa aagatttgta gtggcaccca tagattacgg gtctgcgttg aactacgcct
      241 gaaatgacaa tagagaagat aatagaaaac gacttttatt accctcccca gctttgttta
      301 gaactttccg tagttatctt tgagaatcac catcggatgc cgttttgaca tctggaactt
      361 gataagtaat tcacgggcaa tcatgtcacg gtcctgctga ttatcgggaa gatccatctc
      421 tttgggaacc gtaacccaac cattgatatt tctgtcaaag acagcaggac ggtcttcttc
      481 gtaacccatg tgaacatctt ccagccagtt tagatcctgc caattcatca cttccagata
      541 ttttttcaaa aaaggcgtta gacgctcata gtccggtaca aggttcacat catagcggta
      601 gccgatatgc tgaccaattt ctttttctct ttcggagcct aaatcttctg gaattaatgt
      661 gttattttca ctcatttcaa gtttaaaatt ttaagtttca agtattattc aagtttcaag
      721 ttccgggcat caggcaacaa ggtttgtttc tcattactca gtacttctaa ctgcctagag
      781 ctcagtagcg attcatgtcc ggtcggccaa cagtgtgctg tgagccggcc aaaggaactt
      841 gtgccaaagc tgaagcttcc attgtcaatg ctgccaagtc ttccggttca agggaatgga
      901 cgttagtttt accgcatgca cgcgcaaaca tctggcactc tattgtcaaa cagtgcagga
      961 agttgtatac ccgctccgca gctttgtcgg ggtcaagacg ctttcttaat tcggggtctt
     1021 gcgttgcaac gccaacagga caacgtccag tatgacagtg ataacagtat cctgcttcaa
     1081 cacccatttc cttttcgtaa tcagcttccg gaatgtcctt gttgcagttg agggccatca
     1141 ttgcagagtg cccaattgca actgcatctg ctcccagagc gattgctttt gctacgtcag
     1201 caccattcct gataccgccg gcatagacca gcgatatttt tcctgtataa cccaaatcgt
     1261 caaatgcttt gcgggcctga cgaatggcag caatacctgg gactccagtt tcttcggtag
     1321 ccaggtgcgg acccgctccg gtggaacctt ccataccgtc aatatatatg ctgtccggat
     1381 ttgtcttgag cgccattcga acgtcatcat aaactcgtgc cgaaccaagt tttagctgga
     1441 tcggtatttg agcatcggtg gcttccctga tttcgtttat cttcaaagac aaatcatccg
     1501 gaccaagcca atccggatgc cgtgccggag aacgctggtc aatgcccgca ggcagggaac
     1561 gcatttccgc cac
//
LOCUS       DTVD01000188            6413 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3749_length_6413_cov_283.595156, whole genome
            shotgun sequence.
ACCESSION   DTVD01000188 DTVD01000000
VERSION     DTVD01000188.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_6413_cov_283.595156"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            313..705
                     /locus_tag="EYO68_06560"
     CDS             313..705
                     /locus_tag="EYO68_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC07501.1"
                     /translation="MSDNTQLPDWIYNVNLESQGIARKLADGINTRIFVGENTMLSVV
                     KIESNSSGNIHSHVEEQWGFLIEGECTRIQGDEEFKAKAGDFWHTPSGVSHGIRTDSV
                     GAVVLDIFSPPRAEYKNAGEGFGQAQKG"
     gene            713..1480
                     /locus_tag="EYO68_06565"
     CDS             713..1480
                     /locus_tag="EYO68_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HIC07502.1"
                     /translation="MRKNKLKQLWKEGKTAINAWLTIPSAWTAETLAHTGFDAITIDM
                     QHGLADYQTTLSMLHAISTTDAVPLARVPWNEPATIMRLLDAGVYGLVCPMVNSREEA
                     EAFVGACRYPPLGFRSYGPIRANVYAGDDYFENANQTVITLAMIETTQALENLEEIVT
                     TPGLDGVYVGTVDLSISMGLAGLGDLNDKKLQNALNIIMTQINKHDCIAGIHASTPES
                     AEKLSEQGFRLITPVNDTNLLRGAAINALEETRKWIG"
     gene            complement(1649..2638)
                     /locus_tag="EYO68_06570"
     CDS             complement(1649..2638)
                     /locus_tag="EYO68_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIC07503.1"
                     /translation="MSKLNEMIREFREDIGQSIEGLALLMQLEPDEFARLEEDWIPPD
                     DILKRLCALFEWNYNDIKRLADKTPSVKSKKKELEKSDSSLAEETSAKTEVPQFAKMI
                     QEARITAGQNTLGIATLLSIAPDYYEEFESGLIPPDDLLRKLCSLYGWNYKQIQQKIN
                     SQSPILYGTRQPPLPVKEMQARLPKIELPEIPDIPEGVTLHELIQQSRIEAKQNVEGI
                     SLLLQISTEYYEQIEAGEVFPEQELLKRICSLFGWNYHELLQREKSSQLSKLQPAVTS
                     LDSSDSSITETKLREIQEEIAANWHQISREQQETILTHLELVNKMMEHWKNED"
     gene            complement(2686..3426)
                     /locus_tag="EYO68_06575"
     CDS             complement(2686..3426)
                     /locus_tag="EYO68_06575"
                     /inference="COORDINATES: protein motif:HMM:PF04140.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A Kdo2 1-phosphate O-methyltransferase"
                     /protein_id="HIC07504.1"
                     /translation="MKLEKKLVSSGYWLFRWRSYFPIVIILFFIPAMGEYEYVGGSRE
                     MTTTWGLFCLIVGLLGLLFRIMVVGHTPQNTSGRNTREQIAEVLNTSGWYSVVRHPLY
                     LGNYTMGLGISLFPYSWWMPVIYTFAFALYYERIMIAEEDFLRTKFGDDFEKWSAETP
                     GFFPDFSKWDSPSLNFSFKNILRREYSSLFALIFCFTAFDLVGNYLVVQKPYIVPMWN
                     NLFWTTLAVYLILRTLKRHTQILDVKGR"
     gene            3719..4864
                     /locus_tag="EYO68_06580"
     CDS             3719..4864
                     /locus_tag="EYO68_06580"
                     /inference="COORDINATES: protein motif:HMM:PF11383.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HIC07505.1"
                     /translation="MKIKGLLKLLSAFIISIAWNNSAQAGSINSGLGPLEIRPQFIVS
                     QPFLAMTPENSSTLKGGESKLSVGIEIANTFVNTQGPTEQITKKEVARGLTKEDFYDK
                     NGDAGKGYSLYLDAETKRKNLKYNYGISDSLEFKLEIPFLSFDGGFMDSSIESVHSLI
                     GISNFKQGGAYRALSERNKYDYYVVKDGEFVYASNQIIYNVRGEPSMGLKWNLSEGGN
                     VMPAVTLKLSYKFANSDRSGEQKLVRSGGVDWGHYLILSKGFDKWIVYFGDGKTRIGQ
                     NLGFASSMSHQFMSLEYRIEEEVSFVFQSVSQSSIFQETAASPRSTSGENQEQRNSNL
                     SVSTSVSAFGYKFVSGPIFWETGFVQDYNNFGNETDFVLFWEMGVQW"
     gene            4858..5517
                     /locus_tag="EYO68_06585"
     CDS             4858..5517
                     /locus_tag="EYO68_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07506.1"
                     /translation="MVRFLLKLFLTVSCLIFFKTTVWSVEQTVVYFAFQYGIQDTGSP
                     APVVGQTDSYLSTLETKHGALKENRQVSPKVLSFDFYKTNGSIASGFGLEVHSYKKKY
                     TFENDPSQVDISAVGFLYGLNFYYRGDFWFPFLGFGTGNYSAKVKEELVTGSSTTYGT
                     VFGQVDKPFYYKFGVRIPLNGLGIILTQQYISADFDVSTENKPLSLGGTATFIGLYYG
                     F"
     gene            complement(5656..6060)
                     /locus_tag="EYO68_06590"
     CDS             complement(5656..6060)
                     /locus_tag="EYO68_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07507.1"
                     /translation="MTFASSFPIQLFASALVGDQQYPMGSHIPTSETLKIICDFVIPE
                     EEKVGLERIEVLINGKVKAIKKLRDVSNADLTAVPQSTASGARPESGRIVIDINKPNI
                     PSSPGYIYLRIFGYKAGKLIPSPIYVAPFYLK"
BASE COUNT         1949 a         1227 c         1331 g         1906 t
ORIGIN      
        1 ttcttaagga tctggacggt ctattgaaat tttcgctgct tttcttccgt gctggttgga
       61 aaatattgga ttgtagaaaa agcgatccat gaggtttaag cgccccattc ttcctcctaa
      121 taataagctt catctgggaa caaacaatgg attgttatgt ttgtcttaga cttaaaatga
      181 atttgattcc aaaaaaaagt aagaaatgat tttcaatatt aaatcgattg gagtattgtt
      241 gaaaaagaaa aagtgccgag aagctttatc caagcccaat aaaacagtca aatgaattat
      301 caatggagat aaatgtctga taatacacaa cttcctgatt ggatatacaa cgtaaatctt
      361 gaatctcaag gtatagcaag aaaacttgca gacggaataa atacccgaat ttttgtagga
      421 gaaaacacta tgctttctgt tgtaaaaata gagtctaatt catctggtaa tattcacagt
      481 catgttgaag aacagtgggg gtttttaata gaaggggagt gcacccgtat ccaaggagat
      541 gaagaattta aggcaaaagc aggtgacttc tggcacactc ccagtggtgt gtcgcatggc
      601 atccgtactg attcggtggg tgcagtggtg ctggatattt ttagtccacc aagagcagag
      661 tacaaaaatg ctggtgaggg ctttgggcaa gcccaaaaag gttagttcta agatgagaaa
      721 aaataagtta aaacaactgt ggaaagaagg taaaactgct atcaatgcat ggttgactat
      781 tccaagtgcg tggacagcgg aaacgttggc tcacactgga ttcgatgcca ttacaattga
      841 tatgcagcat gggttagccg actatcagac aaccttgtct atgctgcatg caatttcaac
      901 gactgatgct gtaccactag ctcgtgtacc ttggaatgag cctgccacca ttatgcggct
      961 cttagatgca ggtgtttatg gtcttgtttg tcctatggtc aacagtcgag aagaagcaga
     1021 agcttttgta ggtgcgtgtc gctatccgcc acttggattc cgaagctatg gtccaatacg
     1081 ggcaaatgtt tatgcgggtg atgactattt tgaaaatgca aaccagacgg tcataacatt
     1141 agctatgatt gaaacaacgc aagctttgga aaatctggag gagattgtga cgactcccgg
     1201 tctggatggt gtttatgtag gaaccgtaga tttaagtata agtatggggt tggccggatt
     1261 gggagacttg aatgacaaaa aattacaaaa cgctctaaac attattatga cgcaaatcaa
     1321 taaacacgac tgtattgcag gaatacatgc aagcacacca gaaagtgcgg aaaaactatc
     1381 tgaacaaggt tttcgtttga taacaccagt aaatgatacg aatttattgc gtggtgcggc
     1441 aataaatgcc cttgaagaga ctcgcaaatg gattggataa atacttttcc aaatagtacg
     1501 aaaaaggttg tctaacaagg tgatgaagtg aagggattac ccagatcact tttcatcatc
     1561 gctctctaaa acaaatatag taaatcttct atctcgtgtc acatcgttgg ggagttggcg
     1621 taggtgtgtt tacatcactt ttaacaaatt agtcctcgtt tttccaatgt tccatcattt
     1681 tgttaaccaa ttccaaatga gttagaattg tttcctgttg ttctctggat atttggtgcc
     1741 agtttgctgc aatttcctcc tgaatttccc tgagttttgt ttctgttatg gatgaatctg
     1801 aagaatccag agatgtaact gcaggctgta atttactcag ttgtgaactt ttttcacgct
     1861 gcagcagttc atgataattc caaccgaaaa gagaacatat tcgtttcagc agttcctgtt
     1921 ccggaaacac ttctccagct tcaatctgtt cgtagtattc agtgctgatt tgcagcaaaa
     1981 gggaaattcc ttccacattt tgctttgcct caattcttga ctgctgaatt agttcgtgaa
     2041 gggtaacacc ttcgggaata tcaggaattt ccggcagttc gatctttgga agtcttgcct
     2101 gcatttcctt gacaggcagt ggaggctgcc gggtaccata taaaatcgga ctttggctgt
     2161 tgattttttg ttgaatttgt ttgtagttcc aaccatatag agaacacaat ttacgcaaca
     2221 agtcatccgg aggaatgaga cctgattcga attcttcata ataatccgga gctatgctaa
     2281 gtaaggtggc tatccccagt gtattctgcc cggccgttat acgggcttcc tgaatcattt
     2341 tcgcaaactg aggcacctcg gttttagccg aagtttcttc tgctaaagaa gaatccgatt
     2401 tttccagttc ttttttcttg gatttaacgg agggagtttt gtcggcaaga cgtttgatgt
     2461 cattataatt ccactcaaaa agtgcacaaa gtcgcttaag aatgtcatcc ggaggaatcc
     2521 aatcttcctc caacctggca aattcatctg gctccaactg catcagcaat gctaatcctt
     2581 ctatgctctg gccaatgtct tcgcggaatt cgcgaatcat ttcattcagt ttggacactg
     2641 ttatctgctg aagttacaat tctgtgattc gagaaagttt tgcaattacc gcccttttac
     2701 gtccagaatc tgagtgtgcc gctttaaggt cctcaaaatc aaatagacag caagagtagt
     2761 ccagaacaaa ttgttccaca tgggaacaat gtaaggcttc tgcactacta aataattgcc
     2821 aaccagatca aaagctgtaa aacaaaatat cagagcaaag aggctggaat attctctgcg
     2881 caggatgttt ttaaatgaaa aatttaatga agggctatcc catttagaaa aatcgggaaa
     2941 gaagccggga gtttccgccg accatttttc aaagtcatct ccaaacttag ttctgaggaa
     3001 atcttcttct gcaatcatga tgcgttcata atagagggca aaggcaaagg tgtatatcac
     3061 aggcatccac caggaatatg gaaacagcga gatgccaagt cccattgtgt aattccccag
     3121 gtaaagcggg tgtctcacaa cagagtacca tccactggta ttaaggactt ctgcaatctg
     3181 ctctctcgta tttcttccgg aagtgttttg cggagtgtgt ccaactacca taatcctgaa
     3241 taacaagccc agtaatccga caatcaaaca gaataacccc caggtagttg tcatttcccg
     3301 actgcctccc acatattcat attctcccat tgcaggaatg aaaaacaaaa ttatgacaat
     3361 ggggaaataa ctcctccagc gaaacagcca gtacccgctg gaaacaagtt ttttttcaag
     3421 tttcaatcag agactccttt aaatttgttt ttaagtgttt ttcaaaagtt taaccattgc
     3481 cgaaagactg ctttcgaaac agatgtttta atttcatgca atgattcttc cagcaggaat
     3541 tgttgcccaa atataatcat tcccaaccag gtaaggaaac aaaatattgc aaatcgaatt
     3601 gatttactgt gacaaatttt tacacaagct aaaaggcata actcttggat tcattttcac
     3661 tgtggttgtt gccaaaattc agggctaccc gagatattta tgtttcctgt ttatttatat
     3721 gaaaataaaa ggtttattaa aattattgtc tgcttttatt atttctatag cttggaataa
     3781 ttctgctcaa gcaggctcaa taaattccgg actgggtccc ctggaaattc gtccgcaatt
     3841 tattgtaagt cagccttttt tggccatgac tccagaaaat agttctactc tcaaaggcgg
     3901 agaatccaaa ctgtctgtcg gaatagagat cgcaaatact tttgtcaaca ctcagggacc
     3961 gactgaacaa atcacaaaaa aagaagtggc ccgcggatta acaaaagaag atttttacga
     4021 taaaaacggg gatgcaggta agggatacag cttgtatctg gatgcagaaa ctaaacgtaa
     4081 aaatttaaag tacaactatg gaatctccga ttcattagaa ttcaaactcg aaattccatt
     4141 tttatctttt gacggtggat tcatggacag cagcatagaa agcgtacata gcctgattgg
     4201 aatttcaaat tttaaacagg gtggagctta tcgtgccctt tctgagcgca acaaatatga
     4261 ctattatgta gtcaaggacg gagaatttgt ttatgccagc aaccagatta tttacaatgt
     4321 acgtggcgaa ccaagcatgg gactcaagtg gaacttgtct gaaggcggaa atgtcatgcc
     4381 agcagttact ttaaaacttt cctacaaatt tgccaattcc gaccgtagcg gagaacaaaa
     4441 attagttcgc agtggcggtg tagattgggg ccactacctg attctgtcca aaggattcga
     4501 taaatggatt gtctactttg gtgatggaaa aaccagaatt ggtcaaaatc tgggatttgc
     4561 gtcgtccatg agccatcagt tcatgtccct ggaatatcga atagaagaag aagtgtcatt
     4621 tgtttttcaa tctgtctcgc aaagcagcat ctttcaggaa acagctgcaa gtccccgctc
     4681 tacaagtgga gaaaaccagg aacagcgaaa ctcaaactta agtgtttcca ccagtgtttc
     4741 tgcattcggc tataaatttg tttccggtcc aatcttttgg gaaacaggat ttgttcagga
     4801 ttacaacaat tttggaaatg aaaccgattt tgttttattt tgggaaatgg gggttcaatg
     4861 gtgagattct tattaaaact tttcctgaca gtaagctgtt tgatcttctt caaaactaca
     4921 gtttggagcg ttgagcaaac agtggtatat tttgcctttc aatacggaat acaggacaca
     4981 ggaagtcctg cacctgtagt tggacaaact gactcatacc tgtctacttt ggaaactaaa
     5041 catggtgcat tgaaggaaaa ccggcaggta agtccaaaag tattgtcctt cgatttttac
     5101 aaaacgaatg ggtcaattgc cagcggattt ggattggagg tccacagtta caaaaaaaaa
     5161 tatacttttg aaaacgatcc ctctcaggtc gatatttctg cagtaggatt cttatacgga
     5221 ttgaattttt attaccgggg agatttttgg tttccgtttt tgggttttgg aacaggaaat
     5281 tattctgcaa aagtgaaaga agaattagta acaggaagtt ctacgactta tggtactgtt
     5341 ttcggacagg ttgacaaacc attttattat aaatttgggg tacgcattcc tttaaatggt
     5401 ctgggcataa ttctcactca gcagtatatt tccgcagatt ttgatgtctc cactgaaaat
     5461 aagcctctct cactcggcgg aacagctacg tttattggtc tttattacgg attttgattc
     5521 aagacagcat ggtaatcgtc taaatgagca cctgcaaaat agcatccgga attcactatg
     5581 cggacatacc agctgtgtta gtggttcatg gtgtacgtcc gttctttaaa tacacaactc
     5641 cggagcagtc ttttgtcact ttaaataaaa tggtgcgaca taaataggac tgggaattag
     5701 tttgccggct ttataaccaa agatccgtaa gtaaatatat cctggactac tgggaatatt
     5761 aggcttattt atatcaataa cgatacgtcc ggattcaggt ctggcaccgc ttgcagtgga
     5821 ttgtgggaca gcagttaggt cagcgtttga aacgtctctt aattttttaa tagcctttac
     5881 tttcccattg attaagactt caattcgttc aaggccaact ttctcttctt cagggattac
     5941 aaagtcacaa ataattttta acgtttcaga agttggtatg tgactaccca taggatattg
     6001 ctgatcacca acaagagctg atgcaaataa ctgaattgga aaggaagaag caaaagtcat
     6061 tctcttcttt aacgctgaaa atatcgcttt ctgactcaac tcttcagcaa caactccaat
     6121 tcgggatttt cccggtacat tccggtaatc atgtgaatca ttagaagaga ttggagccag
     6181 ccaccatccg gattgaacag cttcatctat gaataacagt ttaccgccat accaggacct
     6241 aggaaagtaa tgggatccca atggtgagtt tcaataccaa ttattttatc aacaatatct
     6301 ttatcaaatt taaaatgaga aaactcaaag ggctttcagt aaaaatgaaa gccgggatgt
     6361 gcaaaaataa caagtgcatc tttttgctcc ggttttttta accaactata tag
//
LOCUS       DTVD01000189            1540 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_38705_length_1540_cov_486.117172, whole genome
            shotgun sequence.
ACCESSION   DTVD01000189 DTVD01000000
VERSION     DTVD01000189.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38705_length_1540_cov_486.117172"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="EYO68_06595"
     CDS             <1..1032
                     /locus_tag="EYO68_06595"
                     /inference="COORDINATES: protein motif:HMM:PF02321.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIC07508.1"
                     /translation="FFQDSGTEFNELPVRLAEIGVTRGQLNSKQTELSLIKQVASIYW
                     DLVGLLETIEVKKKAVELSEKLLRDNQARLEAGVLNSTEVRTTETQLMRNRHSLLSSR
                     LDALRIEDQVRAALNLKNLPVGLFPSDKPKTQFAVPENVSVLLEKIYSNDSQIGLTQA
                     SLEQNSYQLEQELNKQKTNMDLSLSYILNGYSKGFFGSTADFSKSKLHGMSATLTWEV
                     PLGDQATVENIQRKRLDQEKLTLQIKDRKSQLESSLHSLLRSLRLIEKEKLTVAAVSK
                     LSKDQLRNEIERFKLGKSTSYLISQFQQDVVEAQQQEILVRIRQEKIRVELLALTGEF
                     NEKYELNQK"
     gene            1152..>1540
                     /locus_tag="EYO68_06600"
     CDS             1152..>1540
                     /locus_tag="EYO68_06600"
                     /inference="COORDINATES: protein motif:HMM:PF13533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIC07509.1"
                     /translation="MYNYLKNCKIIIGVFIIWPFFSLATSHGAELSGSFKTPISLTGI
                     IEPAIILNVQSSVGGRVEVLHVKENQHVPKNQLLLTLNNDTQKRQLELTLLQHKVNEN
                     NVIDRKRQLKLANVQIKVDENNVKDRK"
BASE COUNT          521 a          279 c          321 g          419 t
ORIGIN      
        1 ttttttcagg actctggtac tgaattcaac gaattacctg tacgtctggc agaaattggt
       61 gttacaagag gccagttgaa ttcaaagcaa actgaattgt ccctgattaa acaggttgct
      121 tcaatttatt gggacctggt tggattatta gagacaattg aggttaaaaa gaaagcagtt
      181 gaactttctg aaaaactttt acgcgacaat caggccagac tggaagcagg ggtcctgaat
      241 tcaactgaag tccggactac agaaactcaa ctgatgcgta atcgacacag tttgctttct
      301 tcacgtttgg atgccctgcg tatagaagat caggttcggg ctgcactaaa tttgaaaaat
      361 ttgcctgtgg ggctttttcc ttcagacaaa cctaaaactc aatttgctgt tccggaaaac
      421 gtttccgtgt tgttggaaaa aatctattcg aacgactcgc aaattggttt gacgcaggcc
      481 tctttggagc aaaatagtta tcagttggaa caggaactga acaaacaaaa aactaatatg
      541 gatttgagtt tgtcctatat tttaaatggt tacagcaaag gtttttttgg cagcactgca
      601 gatttcagca aaagcaagct ccacggaatg agtgcaactc taacatggga agtcccgtta
      661 ggcgatcagg ctactgttga aaatatccag cgcaaacgtc ttgatcagga aaaattaact
      721 ttgcagatca aggacagaaa atctcaactt gagtccagtt tacattcgct tttgcgttcc
      781 ttgagattaa tcgaaaagga aaaactgaca gttgccgcag taagtaaact ttctaaagat
      841 cagttgcgca acgaaattga gcgcttcaag ttgggaaaaa gtacgagtta tcttatttcc
      901 cagtttcaac aagatgtggt ggaagcacag cagcaagaaa ttctcgtcag gattcgtcaa
      961 gaaaaaatcc gggttgaatt acttgcttta accggagagt ttaatgaaaa atatgagctg
     1021 aaccagaagt gatggatttg ccacaattat taaacccttt tagacgagta gagaccagaa
     1081 acatgaatga tgagaaatcc gctcatcttt cacagtagat caatgactaa atcagcaaaa
     1141 aaggtattaa tatgtataac tatttaaaga actgcaaaat aattattggt gttttcataa
     1201 tttggccgtt tttcagcttg gcaacatccc atggtgctga gctttcagga tcattcaaaa
     1261 caccaatttc tttaacagga ataatcgagc ctgcaataat attgaatgta caatccagtg
     1321 tgggaggaag agtagaagtt cttcatgtta aagaaaatca gcatgtcccc aaaaaccagc
     1381 ttttgctgac tctcaataat gatacacaga aacggcaact ggaattaacc cttctgcagc
     1441 acaaagttaa tgaaaacaat gtaattgatc gcaaacgtca attgaaactg gcgaacgtgc
     1501 agataaaggt agatgaaaat aatgtaaaag accgcaagaa
//
LOCUS       DTVD01000190            6291 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3870_length_6291_cov_300.188422, whole genome
            shotgun sequence.
ACCESSION   DTVD01000190 DTVD01000000
VERSION     DTVD01000190.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_6291_cov_300.188422"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            48..425
                     /locus_tag="EYO68_06605"
     CDS             48..425
                     /locus_tag="EYO68_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07510.1"
                     /translation="MITDFLHNCGEAEKGFVNSQEWWIMSGSVKVQIFLTSLEDNAEL
                     IVASNLFQYEEQNADINEYILKLNGTLKLKGVCFGIRNKHLILSSIRPVQGLDPEELE
                     WIIASIAVIADEYDDFLIEKFNL"
     gene            complement(616..1260)
                     /locus_tag="EYO68_06610"
     CDS             complement(616..1260)
                     /locus_tag="EYO68_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC07511.1"
                     /translation="MNDTDIIVETIPVGPLQCNCTILGDLVSRKAIVVDPGGDAEILL
                     ERLVELNLQVERIIHTHAHLDHFLASGKMKEATGAKLALHREDLFLWDMLEDQCRMFG
                     IPFEPPPPPDQWLENEEEIDLNDLQGKALHTPGHTPGSMCFLFESQKLLIAGDTLFQG
                     SIGRTDLWGGDFKKIEKSIQEKLYTLDEETSVITGHGESTSIGHEMRANSFVRA"
     gene            complement(1257..3560)
                     /gene="topA"
                     /locus_tag="EYO68_06615"
     CDS             complement(1257..3560)
                     /gene="topA"
                     /locus_tag="EYO68_06615"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIC07512.1"
                     /translation="MAKNLIIVESPAKARTISKFLGEDYFVTASMGHVRDLPSSVLGF
                     DPENDFAPQYEIPKDKKKTVAELKKQINSKTEVYLATDEDREGEAISWHLLEALGLEK
                     RPVKRIVFHEITKSAILNALEKPREVDQQLVDAQQARRILDRAVGYELSPLLWKKIKP
                     GLSAGRVQSVSVHILVEREKEIRKFEPEEYWKIRAEFAEFTAELKKLDGKPAKVVNET
                     EAKSIEDSLKQGNFSVSVIDERMAKRNPGAPFTTSTIQQEASVKLGFSVKRTMVVAQQ
                     LYEGNFNIPDYSGGLITYMRTDSVVLANQALMQAQEVIGSEYGSKFGLKQPRFFKNRS
                     TNAQEAHEAIRPVDFSLKPSAVKESLSSDQFRLYSLVWKRALASQMSAAEIARTTLKI
                     EAGVKRECLFEAQGQRVVFPGFLLAYSEGKNDVDEAVSEKDVILPKVEKDQILPLKKL
                     NLEQLFTKPPPRYTEASLVKKMESEGIGRPSTYAPTISTIQDRGYVEVTPEKRLKPTS
                     IGEVVTDFLTNHFPEIVNLGFTAKIERNFDRIANGEENWVEMIANFYQPFHQRVGEKD
                     ESVTRAEVLKRRELGTDPESGKPVSVSIGKFGPMVQIGTREDEEKPRFASLPSNLNLN
                     EVTFEEAMECFALPLTLGTHESGEEIIVNNGRYGPYVRLGKDFFSLPKGEDPLKTNIE
                     TALEIIREGLEKKAKSTIHDFGEIRVIDGRFGPYIKSGKSNYKIPKGVEPETLDEAAC
                     LKIIAETPPSKGRKGGKRKFTKKEASA"
     gene            complement(3812..4390)
                     /locus_tag="EYO68_06620"
     CDS             complement(3812..4390)
                     /locus_tag="EYO68_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIC07513.1"
                     /translation="MSKESNEENEELEGMASKLLKSRTVVISQQVDAKLTAKVLSQLV
                     LLEQEGPEEPITVFINSPGGEIFSGFAIFDMLNFIACPVTTIVTGFAASMGSILSLAA
                     DKGRRFSMPQAKIMIHQPLLMGYQGRASECEIQAREILKTRDHLVKLYAEHTGKSQKE
                     IKKALDRDNWFSAEEALEYGLIDKVIHSRSEL"
     gene            complement(4684..>6291)
                     /gene="recN"
                     /locus_tag="EYO68_06625"
     CDS             complement(4684..>6291)
                     /gene="recN"
                     /locus_tag="EYO68_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIC07514.1"
                     /translation="KSIMIDAILLVLGHRGDPGMIRTGKELAEVEGIFSVPENPVNGE
                     QFNIRKELELTGINLDDELIVRCIVSRKGRQKRYINGVSVTADFLKSIGRKLINIHGQ
                     HDNQSLLQVSSHLDFLDGFGQLIPLRQQVTEAFQALQAAKKEQLSLQKRFADRAVRID
                     ELKEIIADLNELNFQPTEENELRKEENRLTHVEKLLVLLGQVRSRLNEADGSVIEQLE
                     LSRKLLGEAEQIDSECSKILSGIETALFQIEDSYQELVQYSDKVEDDPERLAWINERL
                     SRIQHYTRKYRLDNADELLTLFEDSKQELDSLEHLDENEQEICEKIEQLRNQTQKFAE
                     LLSRKRHNAAKIMDKAIVDELRQLGMEKAHFETHIVSASFAENREVCTTKGIDQVEFM
                     LTVNPGQDMRSLVKVASGGELSRIMLALKTVLTSLDSVEILIFDEVDTGISGGIAEIV
                     GQKLHSLGERHQTLCVTHLPQIAAFAEHHYFVSKHLTDNETYTKIKLLNTENERIRAL
                     ADLIGGQEITEQTLELAQEMLQSFQIK"
BASE COUNT         1747 a         1383 c         1106 g         2055 t
ORIGIN      
        1 ccaatcaatc tttggagtat ggagaagagt tgaaccagta tagtgcaatg attaccgatt
       61 ttctgcataa ctgtggagaa gcggaaaaag gtttcgtcaa cagtcaggag tggtggataa
      121 tgagtggatc tgtgaaggtc cagattttcc taacgagttt ggaggacaat gccgaattaa
      181 tagttgcatc aaatctgttt cagtatgagg agcaaaacgc agatattaac gaatatatac
      241 tgaaacttaa cggtacccta aaactaaaag gtgtgtgttt tggtatacga aacaagcacc
      301 tgatattaag ctctatccgt cctgttcaag ggttggaccc agaagaactt gaatggatta
      361 ttgccagtat tgcagtcatt gccgatgaat acgatgattt cctgattgaa aaattcaacc
      421 tgtagcaaac tgtttctgcc tctcaaattt aagtttgaac gtatgcactt cttatgttta
      481 ccgttgttac tcctcttcaa ctggataatt cataagactg aattttaaca aaactcttgt
      541 cacacactta gttacgttga agatcttccg gaggctttga tcgggaggca cagatactgg
      601 aactggaagg tcttttcagg ctcgaacaaa agaattagca cgcatttcat gaccgatact
      661 tgttgactct ccgtggccgg taattacaga agtttcttca tcaagcgtgt aaagtttctc
      721 ctgaatcgat ttttctattt tcttaaaatc acctccccac aaatctgttc ttccgataga
      781 tccctggaac agagtatctc cggcaatcag taatttttgg ctttcaaaca aaaaacacat
      841 tgaaccagga gtatgtcctg gagtgtgcag tgccttgccc tgtaaatcat tcaggtcaat
      901 ctcttcctcg ttttccaacc attgatctgg aggcggagga ggctcaaaag gaataccaaa
      961 cattcgacat tgatcctcca acatatccca gagaaataaa tcttcacggt gtaaagccaa
     1021 ttttgctccg gttgcctctt tcatttttcc ggaagcaaga aaatgatcca ggtgcgcatg
     1081 ggtgtgaata atcctttcca cttgaagatt cagttcaaca agacgctcaa gcagtatttc
     1141 agcatctcct ccgggatcaa ccacaattgc tttccgactt accagatcgc ctaaaatggt
     1201 gcaattacac tgcagtgggc caactggaat agtttctaca attatatcag tatcattcat
     1261 gcagaagctt ctttttttgt gaacttcctt tttccgcctt tccttccttt tgaaggaggt
     1321 gtttccgcaa ttattttcag acaagcagct tcatccaatg tttctggttc aacaccttta
     1381 ggtattttgt aattactctt tcctgatttt atataaggcc cgaagcgacc atcaataaca
     1441 cgaatttcac caaaatcatg aattgtactt tttgcctttt tctccaagcc ctccctgata
     1501 atttccagtg cagtctctat gtttgttttt aacggatctt ctcctttagg caaagagaaa
     1561 aaatctttcc ccaatcttac ataaggacca taacgaccat tgttgacgat aatttcctca
     1621 ccgctttcgt gagttcccaa agtaagaggt aaagcaaaac attccatagc ctcttcgaat
     1681 gttacttcat tgagattgag attacttggc aaagaagcaa aacgcggttt ttcctcatcc
     1741 tcacgagtac ctatttggac cattggacca aatttaccaa tgctcacact aacaggtttc
     1801 ccgctttcag ggtcagttcc gagttcccgt cgtttaagta cttcagcacg tgttacagat
     1861 tcatcttttt caccaaccct ttggtgaaag ggttgataga aattagctat catttcaacc
     1921 cagttttctt ctccatttgc tatccggtca aagttacgct caatttttgc ggtaaatccc
     1981 aaattcacaa tttcaggaaa atgattggtg agaaaatctg taacaacttc tccaattgac
     2041 gttggcttaa gccgtttttc tggagttact tcaacatagc cacgatcttg gatagtagat
     2101 atagttggcg cataagtcga gggacgtcca atgccttcag attccatttt tttcaccaaa
     2161 gatgcttcgg tgtaacgtgg cggaggttta gtaaacaatt gttctaaatt caattttttt
     2221 aatggaagta tttgatcttt ttctactttt ggcaagatta cgtctttttc acttacagcc
     2281 tcatccacat catttttacc ttcggagtag gccagcaaaa atcctggaaa aacaactctt
     2341 tgcccttgtg cctcaaataa gcactctctt tttaccccag cctcaatttt cagagtggta
     2401 cgtgctattt ctgccgcaga catttgagat gcaagggcac gcttccagac caggctatat
     2461 agtcggaact ggtcggaact caaactctct ttgactgcac ttggtttcag tgaaaagtcc
     2521 actggcctaa ttgcttcgtg agcttcctgc gcatttgtgg agcggttctt aaaaaaccga
     2581 ggttgcttca aaccaaattt tgaaccatat tctgagccaa taacctcttg tgcctgcata
     2641 agcgcctggt ttgctagcac tactgaatcg gtacgcatat aggtaattaa gccaccgctg
     2701 taatcaggaa tattaaaatt accttcataa agctgctgtg cgactaccat tgttctttta
     2761 accgaaaatc ccagtttgac tgatgcttcc tgctgaattg ttgaagttgt aaatggtgca
     2821 ccaggattgc gttttgccat acgctcatct ataacactta cagaaaaatt accctgcttc
     2881 aaactgtcct caatcgattt tgcctcagtt tcattaacaa cttttgcagg tttgccatcg
     2941 agtttcttca attctgcagt aaattctgca aattctgcac gaattttcca atattcttct
     3001 ggttcaaatt ttctgatttc tttttcacgc tcaaccaaaa tatgaacaga cacactctgc
     3061 actcttccag cagaaagccc gggtttgatt tttttccata ataatggaga cagttcataa
     3121 ccaacggctc ggtccaaaat tctgcgtgcc tgctgtgcgt caaccaattg ctgatctacc
     3181 tccctcggtt tttccagtgc atttagaatc gcagactttg tgatttcatg aaatacaatc
     3241 cgcttcaccg gacgtttttc caaaccaagt gcttcaagaa ggtgccatga aattgcttct
     3301 ccttcacggt cttcgtctgt tgcaagatag acttccgttt tactgttgat ttgttttttc
     3361 agttcagcaa ctgttttttt cttgtccttt ggaatttcat actgaggagc aaagtcattt
     3421 tcaggatcaa agccaagcac acttgagggc agatctctca catgccccat tgaggcggtg
     3481 acgaagtaat cttctcccaa aaacttactg atagtccggg cttttgccgg tgattcaaca
     3541 attattaaat tttttgccat aattattttt tcattcagca aatgttgaaa tgtattctat
     3601 aaaatttgtt ttcataaaat gaggaggatt aaaaaaaagt aaaatcaaca acagaggtga
     3661 tagacatttt tgtaatttta ttttctgtga ccttttcaac tgtctaccaa gattgactca
     3721 ctggaaaaaa tagaaacact aattttgtta ttccagtgaa aactggtatg agaaattgat
     3781 acgtaattca agattgctga aatacctatt tttataattc tgaccgagaa tgtattactt
     3841 tgtcaatcag cccgtattca agcgcttctt cggcagaaaa ccaattatcc cgatcaagcg
     3901 ctttttttat ttctttttgg ctttttcccg tatgttcggc atatagctta actaaatgat
     3961 ctcgagtttt caaaatttca cgtgcctgaa tttcacactc agaagctctc ccctggtaac
     4021 ccattaacag gggttggtga atcattattt tagcttgggg catggaaaag cgtcgcccct
     4081 tgtctgcagc caaactcaag attgacccca tgcttgcggc aaatcctgtt acaattgttg
     4141 ttaccggaca tgcaataaaa ttgagcatat caaaaatggc aaatccggaa aatatttctc
     4201 ctcctggaga atttatgaaa acagtaattg gctcttccgg accttcctgt tcgagtaaaa
     4261 ccaattgact caaaactttt gcggtcaatt tcgcatcaac ttgctgggag atcacaactg
     4321 ttctggactt gagcaacttg ctcgccatgc cctccaattc ctcattttct tcgttacttt
     4381 ctttgctcat ttattgttcc tgtgaagtca tacttgagtt tttatctatt aattagctct
     4441 tctaccaaat tatgggaggg gaaaagcgtg tgcaagtttt ttgttgaatt atctattaga
     4501 aaagcaaata taagctgccc ttttgaaaaa catgaactgc aagataataa ctgcaatatc
     4561 accaaaaact gacacatata gtattgtttt taaattattt atttttctac tccctgaaat
     4621 gcagctatgg ttttattcca atagttaact tcgctaaagg ttccattatt tgtctgcaaa
     4681 aaattatttt atttgaaaac tttgcaacat ttcctgtgcc agttccaagg tttgctctgt
     4741 gatttcctgc ccgccaatta aatcggcaag agcccggatc ctttcattct cagtgttgag
     4801 aagttttatt tttgtataag tctcattatc agtcagatgc ttgctgacaa aataatgatg
     4861 ctctgcaaaa gctgcaatct gcggaagatg tgttacgcac aaagtctggt gcctctctcc
     4921 aagtgaatgt aatttttgtc ccacaatttc tgcaatccct ccgctgattc cagtatcaac
     4981 ttcatcaaaa atcaatattt caactgagtc cagtgatgta agaactgttt taagtgccag
     5041 cattatacgt gataattctc ctccggaagc aacttttaca agactgcgca tatcctgtcc
     5101 tggattgaca gtcagcataa attcaacctg atcaatcccc ttagttgtgc atacctcacg
     5161 gttttccgca aatgaagctg aaacaatatg agtttcaaaa tgtgcctttt ccatccccaa
     5221 ttgacgtaat tcatcaacaa ttgccttgtc cattattttt gccgcattat gccgcttcct
     5281 ggagagaagt tcagcaaact tttgcgtttg attcctcaac tgctctatct tttcgcaaat
     5341 ctcctgctca ttctcatcaa ggtgttcgag tgaatccaat tcctgctttg aatcttcaaa
     5401 cagtgtcaac agttcatcag cattgtccaa cctatattta cgagtataat gctgtattcg
     5461 actgagtctc tcatttatcc atgccagacg ttcaggatca tcttctactt tgtcgctgta
     5521 ttgaaccagt tcttggtaac tgtcttcaat ctgaaaaaga gctgtttcaa ttccagaaag
     5581 tattttcgag cattcagaat ctatctgttc tgcttcaccc aaaagttttc gtgacaattc
     5641 caattgctct attactgatc cgtccgcttc attgagtcga gaccgaacct gtcccaaaag
     5701 cactaatagt ttttctacat gtgttagccg gttctcttct tttctaagtt cattttcttc
     5761 tgtaggctgg aaatttaatt cgttcaagtc tgcaataatt tcctttagct catcaatacg
     5821 aactgcacga tcagcaaatc gcttttgcag tgatagctgt tctttttttg ctgcttgcaa
     5881 agcctgaaaa gcttccgtca cttgttgacg taaagggatc aattgaccaa aaccatcaag
     5941 aaaatccaag tgggatgaaa cctgcagcaa agattgatta tcgtgctgtc catgaatatt
     6001 gattaattta cggcctatac tttttaggaa atccgcagtt actgatactc cgtttatata
     6061 tcttttttga cgacctttac gagaaacaat acaacgaaca attaattcat catccagatt
     6121 aataccagtc agttccagtt ctttccgaat attgaactgc tcaccattaa ctggattttc
     6181 tggtacagaa aatatccctt caacttcagc cagttcctta cctgttcgga tcatgcctgg
     6241 atcacctcga tgaccaagta caagcaaaat agcatcaatc ataattgact t
//
LOCUS       DTVD01000191            1538 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_38806_length_1538_cov_193.431558, whole genome
            shotgun sequence.
ACCESSION   DTVD01000191 DTVD01000000
VERSION     DTVD01000191.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38806_length_1538_cov_193.431558"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="EYO68_06630"
     CDS             complement(<1..1099)
                     /locus_tag="EYO68_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07515.1"
                     /translation="MFAPLPGCVEGVGCAVPIAGLISNFYKSTDLVILKTLAKGDIEA
                     TVVQLQKFPIIDEKLLLKVLYYDWDISFLSYDRGIHSGKNDYYQTFENLNTSTINLQS
                     QFYNQHLEIQIGYSSGGTKISKIFDADGNQFSNIQSPLRTWVDHTIGTQIDLTDNHLE
                     PREGIRFELLHNASNYGLSELSDYDVNSLNLTTYIPFLGSDTLLINVFQSRSYISEHG
                     LTDENALRNKFSLGCDFEMEADACRNTETKRINYWRKRNQSGKATALGGLNRMRAYSL
                     GRFYAGNSSNYALEYRLNFSEKQTPINWIIMGGMRTVLQASFFYEVGGVSDDISQLHK
                     NMRTSFGVGFRAIISGLIYRFDLAKGDDGIAP"
BASE COUNT          445 a          313 c          299 g          481 t
ORIGIN      
        1 ttggtgcaat gccgtcatct ccttttgcaa ggtcaaaacg gtaaatcagc cctgatataa
       61 ttgcgcgaaa accgactcca aaggaagttc tcatattttt atgcaattga gagatatcat
      121 cagaaacacc gcctacttca taaaagaacg atgcttgaag aacagttcgc attccaccca
      181 taataatcca gtttattgga gtctgttttt ccgaaaaatt aagacggtat tccaaagcat
      241 agtttgaact gtttcccgca tagaaccttc ccaggctgta ggcacgcata cggttaagtc
      301 cgcctaatgc agtagccttg cctgattgat tcctttttcg ccagtagttt atgcgttttg
      361 tttcagtatt ccggcaggcg tcagcctcca tttcaaaatc gcagcccagg ctgaatttat
      421 ttcttaatgc attttcatct gtcaatccat gttctgaaat gtaggatcgc gattgaaaaa
      481 cgttgatcaa caaggtatcc gaccccaaaa aagggatgta agttgttaaa tttagagaat
      541 taacatcata gtcactcagt tcgctgagtc cgtaattcga tgcattgtgc aatagttcaa
      601 atcgaatccc ttcacgtggt tccaaatgat tgtcggtcaa atcgatctgg gttccaatgg
      661 tgtggtcgac ccaggttcgt aaaggagatt gaatatttga aaactgattc ccgtcagcat
      721 caaaaatttt tgaaatcttt gtgcctcctg aggaataacc gatttgtatt tccaggtgct
      781 gattataaaa ctgggattga aggttgattg tggaagtgtt gagattttca aaagtctgat
      841 aataatcgtt ctttcctgaa tggatccccc tgtcgtagct cagaaagctg atatcccagt
      901 catagtacaa aacctttaag agcaactttt catcaataat gggaaatttt tgaagttgaa
      961 ctacagttgc ttcaatgtcc ccttttgcca gcgttttgag tatcacaagg tccgtgcttt
     1021 tatagaaatt tgagattaac cctgcaatgg ggacggcaca tcctactcct tcaacacatc
     1081 ctgggagcgg agcaaacatc catcccagtt ctttatctgc ttctcttttg gttctctcag
     1141 gtggaaattc ataagaatat actacagacg gtatcaaaaa acagagaaga aacaataatt
     1201 tggtttttct cttcagtaag gccccactag cagtcagaaa tgcagaaatc ataggagagc
     1261 agaagttaat atattgattt tctttttctt cataattaat tttcatctag aacatatacg
     1321 agcttagcct gcatttttcg atttttcagg ttgtcaatct gtgatttaag tttgtccaag
     1381 tcagtcttga ggtttttaaa caagagaaga ggattttgaa tatccggcag ctcccgtaaa
     1441 ttttcgctat tgtaacccat aaaagatact tagtcatttt taaagttgcg gaagcacaaa
     1501 ctgcggcctg cggcctgcta tacctcttaa aagtcgtc
//
LOCUS       DTVD01000192            6198 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_3955_length_6198_cov_376.646101, whole genome
            shotgun sequence.
ACCESSION   DTVD01000192 DTVD01000000
VERSION     DTVD01000192.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3955_length_6198_cov_376.646101"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="EYO68_06635"
     CDS             <1..1016
                     /locus_tag="EYO68_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIC07516.1"
                     /translation="AQKRIEITMVATWGRDFPGLGTGAQRFAKRLSDMTDGRMNVKYY
                     AANERVKAFDSFDEVASGNAQMYHAADYYWKGKHPGWAYFTAVPFGFVFSEINSWINF
                     GGGQELWDELAGSFGLKCLSCGNTGVQMGGWFRKEINSADDLKGLKMRIPGLGGDVMA
                     KLGASPVSLPGGQIYEALISGAIDATEWVGPWNDSFYKFYEAAKYYYYPGMHEPASML
                     SLGMNKSWWEGLSNSDQLVIKAASHSENDVMMSEYNAKNGFYLEKLIREQGVQLRRFN
                     DDVYDAFGEAAEDVFSEARAHSNLAERIHASFVKTRQEVGSWMNISDQEYLRQRNRVL
                     GVT"
     gene            1271..2221
                     /locus_tag="EYO68_06640"
     CDS             1271..2221
                     /locus_tag="EYO68_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIC07517.1"
                     /translation="MLFLVNNYLIFWKGWPGLWNFFAHHELFGISALRIPLSPDAQIL
                     GWIQTIILILLILVIILFVLKTPIRTLSEDSRIFTRFAAYLTRACFWAVFIVGCVDIV
                     ISFLRVEDLLSPMFGKVMAVELGRSVFRGTYVHYPLIILSFVIAFFVRGLGFTWLALL
                     VVIAEFQIVISRFVYSYEQAFMGDLVRMWYAALFLFASSYALITEGHVRVDVLYSRFK
                     SKTKALSNAIGCVILGAPLCLVILTTGMWGKGNSLNSPILSFEVYQQGFGMYTKYLMV
                     GFLVVFAVCMLVQFMGYFLNSTAELIEGQMNSDQKLKNNP"
     gene            2245..4911
                     /locus_tag="EYO68_06645"
     CDS             2245..4911
                     /locus_tag="EYO68_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIC07518.1"
                     /translation="MEVFFLLLLVLLMVSALTSGFPVAFSLPGSAILSIGIAALCGYV
                     FEGNASAYFVQDGPLEWLSAGVTNFRSLYWDVERDTLIAIPLFIFMGIMLQRSKIAED
                     LLVTMAQLFGPIPGGLGISVVFVGALLAATTGIVGATVIAMGLISLPAMLEHNYSKPL
                     ATGTICASGTLGQIIPPSIVLIILADQLSNAADIANTSRIANYKLATGEFVMPSEFDV
                     TSASAGEMFIGAFIPGIVLVGLYMVYILISALLKPETAPPIPYEGKIDGKFIIKVLMG
                     LVPPLALIFTVLGSIIMGIATVNQAGAIGAIGAMIMGGYKLTEGQKNSFYPAILAVIS
                     TAIIVITLNFYQLNIRNIRDSEEGIAMIIATIAVIGLLIAVFWSAWRTFRIEDALRGV
                     MLETSKTTSMVFIILLGATMLTSAFRGFGGEELVKEFLSSIPGGFWAQFMVVMAVIFI
                     LGFFLDFIEIAVVVVPLVAPILMADPSANITAVWLGVMIGINLQTSFLTPPFGFALFY
                     LRGVAPPEVKTLHIYRGVVAFIILQLVGLAIAGYFPPLVNYLPNRMYLTSDNAPPPIN
                     PKLQKCIEEITFPFYAEHENDIRAGVDAISQVNVEYLPDKYKKALKTSQLQVLATFDL
                     VEAVHQSDQHLNEFIPSYEDLHQLVRRTQFEVRKIEYDIHELEQRKMRLERNVNSVDA
                     LIMKQIGESIETFQGMIDELEKKIPSQWLSEREKFEKLNKEARSARQKYRRNSDSAYE
                     PLSQLGAILLQTPMLINIENQLKSIKSVIEEEQPDSAMQRIKEIESSLGSIAGASPIK
                     SKFSKARRALKGKKPNVENALKHWQNGMAIYFQEINWRQLAVNELAQPLANYELLLKD
                     SIGLRMQKKLNKDQALAVATCKSSHEDISLFF"
     gene            4947..6053
                     /locus_tag="EYO68_06650"
     CDS             4947..6053
                     /locus_tag="EYO68_06650"
                     /inference="COORDINATES: protein motif:HMM:PF03492.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIC07519.1"
                     /translation="MAGRTESLQNITMSGGGAYSLATKGAEDVINAATQIVLEALDSI
                     NISENQDLFTFSDMGTADGGTSLKMVGNLINAIQKNNPGISFNIVYADQPKNDFNGLV
                     QTVLGLGHFPSYLESTENVFPLFSANSFYKQILPNNTLDFGFSATAMHWLSGKPCNLS
                     NHVHMVGADGEEYLRFSEHGKKDWETILLHRAHELKQGGQLVLLNFCRDENGKYLGNT
                     TGVNMFTNFADIWQDFLDQGRIRPEEYRNMTLPQYYNTVEEFSAPLKNSESSVYLAGL
                     RLKKIETRITPCPFAEAFKDHQDAQRFAEEYIPTIRSWNESIFFGALDIQRPLKERKK
                     IIHDYYQTYQKQVQESPELHRMDYVHAFIVIEKI"
     gene            complement(6082..>6198)
                     /locus_tag="EYO68_06655"
     CDS             complement(6082..>6198)
                     /locus_tag="EYO68_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07520.1"
                     /translation="SMATGYSGVGNPLYFGDNTWMLFGDAKESLNKVAALLI"
BASE COUNT         1728 a         1299 c         1347 g         1824 t
ORIGIN      
        1 ttgctcaaaa gcgcattgaa atcaccatgg tagcaacttg gggacgtgat ttccctggac
       61 ttggtacggg agcccaacgt tttgctaaaa gactatcaga tatgactgat ggtcgaatga
      121 atgttaaata ttatgctgca aatgaaaggg tcaaagcttt tgattctttt gatgaagtag
      181 cctctgggaa cgcacagatg tatcatgctg cagattatta ctggaaaggt aagcatcctg
      241 gctgggccta ctttacagcg gttccatttg gatttgtctt cagtgaaatc aattcctgga
      301 tcaactttgg cggaggacag gaactatggg acgaattggc gggcagtttt ggcctgaaat
      361 gcttatcctg cggaaacacg ggtgtacaga tgggaggctg gttccgtaag gaaatcaact
      421 ctgcagacga cctgaagggg ctcaaaatga ggattcctgg tttgggcgga gacgttatgg
      481 caaagctagg tgcctctcct gtctcacttc caggtgggca aatatacgag gccttgattt
      541 caggagcaat cgatgccaca gagtgggtcg gtccctggaa tgacagcttt tataaattct
      601 atgaagcagc taagtattac tactatccag gtatgcacga gccggcatcc atgttgtctc
      661 tcggcatgaa caaatcgtgg tgggaaggtc tttcaaattc tgatcaactc gtcataaaag
      721 ccgcatctca ttctgaaaat gatgtcatga tgtctgagta caatgcgaag aacggttttt
      781 atttagaaaa actgattcgt gagcagggtg tccagttgag acgctttaat gatgatgtct
      841 acgacgcctt tggagaagca gctgaagacg tatttagtga agcccgtgca cacagtaatc
      901 ttgccgagcg cattcatgct agctttgtca aaacacgtca agaagttgga agctggatga
      961 atatttctga ccaggaatac ctcagacagc gtaaccgtgt gcttggtgtt acgtgattaa
     1021 aggtcactac tcaagtctga agacggtatc ttaaactgta taccgtcttc ttactctcca
     1081 ctaacatttc agttctccat ctgattccta aatattctgt tctaatgatt ttttaaaatt
     1141 tttccggcca aatcatatca tttaaaatcg atctttcagc ggacataaaa cattgactga
     1201 tactattcca aactctcttt gggttcctag actactaaga atttttgcct ggtctacggt
     1261 cagtctgacc atgctttttt tggtgaacaa ctacctaatt ttctggaaag gatggcctgg
     1321 tttatggaat ttttttgctc atcatgaatt gtttggaatc tcagctctca ggattcctct
     1381 aagtcctgac gctcagatat tggggtggat tcaaaccatt atacttattt tacttatact
     1441 cgtgatcatt ctttttgttc tcaaaactcc cataaggaca ttaagtgaag attccagaat
     1501 ttttaccaga ttcgcagcct atctgacacg agcatgtttc tgggctgttt ttatagtcgg
     1561 ttgtgtcgac atagtaattt cctttctgcg tgtagaagac ctattgagtc ccatgttcgg
     1621 gaaagtgatg gcggttgagc tgggacgttc agtttttcga gggacctatg tgcattaccc
     1681 tctgataatt ttaagtttcg taattgcttt ttttgtaaga ggtcttggtt tcacttggct
     1741 ggcactattg gtggtgattg ctgaattcca gattgtcatc tcacgtttcg tttattccta
     1801 tgaacaggct ttcatgggag acctggtgcg tatgtggtat gcagcactct tcctttttgc
     1861 aagttcttat gcattgataa ccgagggaca tgtgcgagtg gatgttttgt attcacggtt
     1921 taaatcaaaa accaaggccc tttcaaatgc cataggttgt gtcattctag gtgctcctct
     1981 gtgcttggtt atccttacaa caggtatgtg gggtaaaggt aacagcttga acagtcctat
     2041 acttagtttc gaggtatacc aacagggttt tgggatgtat acgaaatacc tgatggtcgg
     2101 ttttctagtc gtttttgcgg tctgcatgct agtccagttc atggggtatt tcctaaacag
     2161 tacagcagag ttgattgaag gacagatgaa ttcagatcaa aagcttaaaa ataatccctg
     2221 atttaacaac cttaaagaac ctacatggaa gtcttttttc tacttcttct tgtactcctg
     2281 atggtgagtg cacttacttc aggattccca gtcgccttct ctctcccagg ttcagctata
     2341 ctcagtatcg gaatagctgc cctttgtggt tatgtatttg agggtaacgc atcagcttat
     2401 tttgtccagg atggacctct ggagtggcta agtgcaggag taaccaactt taggagccta
     2461 tactgggatg tggaacgaga caccctgatt gcaattccac ttttcatttt catgggcatt
     2521 atgcttcagc gttcaaaaat tgctgaagac ctactcgtaa caatggctca actgtttggt
     2581 cctattccag gtggtcttgg tatttctgta gttttcgtag gagccttgct tgcagccact
     2641 acaggaatag ttggtgctac agtgattgca atgggactca tttcactacc ggcaatgctg
     2701 gaacataact attccaaacc ccttgcaacc ggaaccatct gtgcttcagg gacactggga
     2761 cagattatcc ctccttccat cgtcttaatc atcctggctg atcagctttc aaatgcagca
     2821 gacattgcca acacttcaag aattgccaat tacaaattgg caacaggtga gtttgtgatg
     2881 ccctcagaat tcgatgtcac ttctgccagt gcaggagaaa tgtttattgg agccttcatt
     2941 ccagggattg tgttggttgg actttacatg gtctatattc tcatctctgc attgctcaaa
     3001 cccgagacag cccctcctat cccttatgaa ggaaaaatag atggcaagtt cattataaaa
     3061 gtacttatgg gccttgtacc tccactggca ttaattttta cggtgttggg gtcaatcatc
     3121 atgggcattg ccacagtcaa tcaggcgggc gctattgggg ctattggagc tatgattatg
     3181 ggaggctata agctcacgga gggccaaaaa aactccttct atcctgccat cctggctgtg
     3241 atttccactg caataattgt tatcactctc aacttttacc aattaaacat cagaaacatc
     3301 cgtgattcag aagaaggcat tgcaatgatc attgccacca tcgcagtgat tggactttta
     3361 attgctgttt tctggagtgc atggaggact ttccgtatag aagacgctct gaggggtgtc
     3421 atgcttgaaa cctcaaaaac cacttcaatg gtgtttataa tacttctagg tgcaaccatg
     3481 ctgacatctg ccttcagagg atttggagga gaggaactgg tgaaggaatt cctgtccagt
     3541 atacctggag gattctgggc gcagtttatg gtggtgatgg ctgtcatctt tatattggga
     3601 ttcttcctgg atttcattga aatcgctgta gtcgttgttc ctttagtagc ccccattctc
     3661 atggccgacc cttccgcaaa cataaccgct gtttggctag gggtcatgat tggtattaac
     3721 ctgcaaactt cttttctcac tcctcctttc ggctttgctt tgttttactt aagaggcgtg
     3781 gccccacctg aggtgaagac gcttcacatt tatcgtggtg tggttgcctt tatcatttta
     3841 caactggttg gtctggccat tgctggctac ttcccccctt tggttaatta tctgccaaac
     3901 agaatgtacc tgacttcaga caatgctcct ccaccaataa atcctaagct tcagaagtgc
     3961 attgaagaaa ttacattccc attttatgca gaacacgaaa acgacatccg cgccggagta
     4021 gatgctatca gccaagtgaa tgttgaatac cttcctgata aatacaagaa agctctaaaa
     4081 acttcacagt tgcaggtact ggccactttt gatctcgttg aagcagtcca ccagagtgac
     4141 cagcatctta atgaattcat cccaagctat gaagacctgc atcagcttgt aagaagaacc
     4201 cagtttgagg ttagaaaaat tgagtatgac attcatgaac ttgaacaacg taagatgcga
     4261 cttgaacgta atgtgaattc tgttgatgct ctgatcatga agcaaatcgg agaaagcatt
     4321 gaaacatttc aagggatgat agatgaactg gaaaagaaaa tcccttcaca atggctatcc
     4381 gaaagagaaa aattcgaaaa actcaataaa gaagctcgtt ccgcccgtca gaaatataga
     4441 agaaactcag atagtgcata cgaacctctg agccaactcg gcgctattct gcttcaaact
     4501 ccaatgctta ttaatatcga gaatcaactc aaatccatca agtcagtgat tgaagaggaa
     4561 caacctgatt ctgccatgca acgaatcaag gaaattgagt caagtcttgg aagtattgca
     4621 ggggcaagtc ccattaaatc aaaattttcc aaggcacgtc gtgcattgaa agggaagaaa
     4681 cctaacgttg agaatgctct gaaacattgg caaaatggta tggcaatata ttttcaggaa
     4741 attaattggc gtcaactcgc tgttaatgaa cttgctcagc ccctggctaa ttatgagttg
     4801 ctgctcaagg attcaattgg attaagaatg cagaaaaaac tcaataagga tcaggcctta
     4861 gctgttgcta cctgcaaatc ttcacatgaa gatatctctt tgttttttta aaccaaattt
     4921 ttaataaccc actttaagtt ttcctcatgg ccggtcgtac agaatcactc cagaacataa
     4981 ccatgtctgg aggtggagca tattcacttg ccaccaaagg agcagaagat gtaataaacg
     5041 ctgctacaca gatcgtgctg gaagctcttg actcaataaa tatttctgag aatcaagatc
     5101 tttttacttt ctcagacatg ggcactgccg atggggggac atcgctaaag atggttggaa
     5161 atttgataaa tgcgattcag aaaaataatc ctggaatcag ctttaacatc gtctatgcag
     5221 atcaaccaaa aaacgatttc aatggtctgg tgcaaacagt acttggttta ggtcatttcc
     5281 ccagttacct tgaaagcact gaaaacgttt ttcctctatt ctcagcaaat tccttctaca
     5341 agcaaattct tcctaacaac actttagatt tcggtttctc agcgacggcc atgcactggc
     5401 taagcgggaa accttgtaat ttaagtaacc acgttcacat ggtcggtgca gatggtgagg
     5461 aatatttacg tttttcagag catgggaaaa aggattggga gaccatctta cttcatcgtg
     5521 cacatgaact taagcaggga gggcagctgg tattgcttaa tttctgccgg gatgaaaacg
     5581 gcaaatacct gggtaacacg acgggtgtga acatgttcac caactttgcc gatatctggc
     5641 aggatttttt ggatcaagga cgaatccgac ccgaagagta ccggaatatg actctgccac
     5701 aatattacaa cacggttgag gaattcagtg ctcccttgaa aaattccgaa agttcagtct
     5761 atctcgcagg attgagactc aaaaagattg aaacacggat tactccatgt ccctttgcag
     5821 aggcattcaa ggatcaccag gatgcacaac gatttgcgga agaatatatc ccaacgatca
     5881 gaagttggaa tgaaagtatc tttttcggtg ccctcgacat tcaacgtccg ttgaaagaac
     5941 gtaaaaaaat catccatgac tactatcaaa cctaccaaaa acaagtgcag gagtcacccg
     6001 aattgcaccg tatggattac gtgcatgctt tcatcgtcat agaaaaaatc tagaacgaaa
     6061 atctcaacag aaatttaaat ttcaaattaa aagagcggca accttgttaa ggctttcttt
     6121 cgcatcacca aataacatcc aggtattgtc tccgaaataa agaggattgc caactccaga
     6181 atatcctgta gccatact
//
LOCUS       DTVD01000193            6165 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4000_length_6165_cov_260.627005, whole genome
            shotgun sequence.
ACCESSION   DTVD01000193 DTVD01000000
VERSION     DTVD01000193.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_6165_cov_260.627005"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="EYO68_06660"
     CDS             complement(<1..1540)
                     /locus_tag="EYO68_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07521.1"
                     /translation="MNYFRILTAIFFTLFLFALSGGAQESTSGNTNSSPSTDDSNINY
                     NFHKVPSGDNLKVFHDAVIGHIVNNKGIQDFYSIPAVETQWLATILVSETKWHEQMQK
                     LEQDYRKAFQERAQLKSGQNDRAIRIENYATAIAKIENEIAEIQRQIVQINVDQQVYI
                     NGLRETPVTSLVAIKTLYTPDLMSSKKKLDVLNSSIFKSMEKPVLAHISAAYGNSMNI
                     PFQAGHIRVTYLYPENITHFDSQANEYVYLFLRVEAYPFSSGRSKAEIADSRSASVNV
                     LADIEQIENYLKTENVGDKRLLDWLKKESNYQNFNNGHLINKIQGNLSDFKIFSNGLK
                     QSISELTSNAQNLGAKRDSLIGSGEEERIESEYKRTKDLYHQFYRNRKVLTYEKYTLE
                     NDVMFSLFLIEGDAAKNNGSTKQKQMIDSNIADNIPISGRPLKDIFADILITANQKSK
                     LDLQNYRERIYRSNDEQTQLIQGELAWEVKNEEFSILKLTRGNVGSRSHFVVHLAKRS
                     ELVSK"
     gene            1916..2116
                     /locus_tag="EYO68_06665"
     CDS             1916..2116
                     /locus_tag="EYO68_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIC07522.1"
                     /translation="MPLYDYQCQQCTNGFTELRRSSEMDTQIDCPECGSRETIRSISS
                     FAVGGSVSGIPSVSAPSRSPFS"
     gene            2318..3676
                     /locus_tag="EYO68_06670"
     CDS             2318..3676
                     /locus_tag="EYO68_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIC07523.1"
                     /translation="MDASIRPKGILDFLSQQEIRQLSDPQNGGLNELFRRCALAVLNS
                     DSQTDSGKEMLAGFPDFSIEVVQQTRSIKLELRNAPPKAFVNGELIQGINEHLFSVLR
                     DIVFVGTELSNKRGFDLKQSSSITDFVYHILRNTGLLRDLIEPNLVVCWGGHSIEETE
                     YQYTKKVGYQLGLRELNICTGCGAGAMKGPMKGATIAHAKQRYNNGLYIGITEPGIIA
                     SESPNPIVNSLVVMPDIEKRLEAFVRTGHGFIVFPGAVGTTEEILYLLGILMHPKNAE
                     QPFPLILTGPKESKDYFDQIDGFIGATLGAEAQRFYEIIIDDPEKVAQRIQSGIRKVR
                     EFRRKLGDAFYYNWMLHIDQDYQWPFSPSHAAMSALNLHYAQPKHELACNLRKMFSGI
                     VSGNIKEEGVLSIENNGPFQISGDPELMQELDKLLSTFCSQRRMKLSRDTEYKPCYEI
                     VA"
     gene            3655..4344
                     /locus_tag="EYO68_06675"
     CDS             3655..4344
                     /locus_tag="EYO68_06675"
                     /inference="COORDINATES: protein motif:HMM:PF04752.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIC07524.1"
                     /translation="MLRDSCLIKELTSVADANQSFEWGSGKEADSIRTLTSQEMVVSV
                     QNILKGHNLEEGVWVFGYGSLMWNPDFKVAEKISGIISGYHRRLCLKSIVYRGTPDYH
                     GLVFGLDKGGSCQGMAFRIAPENLDSELQKVWEREMFAETYIPTWVCVKTENGDVSAI
                     TFVINTKHEHYVPDLDLEKVAERVVLAEGKCGSCHDYVQNTVKFLHQLELRDPVLEQL
                     LTLIEYPQISV"
     gene            4365..5351
                     /locus_tag="EYO68_06680"
     CDS             4365..5351
                     /locus_tag="EYO68_06680"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07525.1"
                     /translation="MNKPISKRRISVWLKLIIAFILLGVGFAGGFKLYEKGNEAGCFP
                     LEDDIVPVEIIPFRADHLQVIVLGDTGTGNEDQLKVADGMAKVCDQAGCDFVLLLGDN
                     FYPNGVKSILDKQFYTKFEQVYNKIKKPFFAVLGNHDVKQNAFTQIMYSLRSDYWRMP
                     NYEYSFETAQARFYGLNTNCPFSFERLRNKLNRDDVELKANADKLPWTIAFGHHSVYS
                     NGTHGDTDVITRNYWNWILDGRIDLYLAGHNHHLSLLQYGETSTEYVISGAGGAHYRT
                     NSEREKLNKSEASSVFTYNDTGFVWLDIRSEKLLIRFHDSTGNILYEFTKTR"
     gene            5395..6165
                     /locus_tag="EYO68_06685"
     CDS             5395..6165
                     /locus_tag="EYO68_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC07526.1"
                     /translation="MSIIQFSHANGFPAKTYSVLFKHLEGHKISAINILAENRKPSEI
                     NWHDMTEDILKCAGQFEEPVVGVGHSLGGVLTLMAAAKKPALFQNIIILDPPLFSSQK
                     RLLISILRAINIEDWVSPSGKSKNRRSHFESKEKAHRYFQANKIFSNFHPQTLKDYVT
                     HGLMLGKQGVELTITVEQEVAIFHKMLTSYPKSIYKVRGTLVYAVKNPVLWASDLRWI
                     KRNFTQLKLIPFPDSHLFPLNYPESTAQIINRCLKCLN"
BASE COUNT         1883 a         1111 c         1266 g         1905 t
ORIGIN      
        1 gttttgaaac taactccgaa cgtttggcca aatggaccac aaaatgactt cttgaaccaa
       61 catttccacg tgtaagtttt aaaatactga actcctcgtt tttaacctcc catgctaatt
      121 ccccctgaat gagctgtgtc tgctcatcgt ttgaacggta tattctttcc cggtagttct
      181 gtaaatcaag tttgcttttt tggtttgccg ttataagtat atcagcaaaa atgtctttca
      241 aaggtctacc actgattgga atgttgtcag caatattaga atcaatcatt tgtttttgtt
      301 ttgtactgcc attgttttta gccgcatcac cttcaatgag aaagaggctg aacattacat
      361 cgttttcaag tgtgtacttt tcataagtca ggacttttcg atttctataa aattgatgat
      421 aaaggtcttt tgtacgtttg tattcagact caattctttc ttcttctcca ctgccgataa
      481 gagaatccct ttttgctcca agattttgtg cattggaagt gagctcagaa atactctgct
      541 tcaggccatt actgaaaatt ttgaagtcac tcagattacc ttggattttg tttattagat
      601 gtccattatt gaagttttga tagttagatt ctttttttaa ccagtccagc aaacgtttat
      661 caccgacatt ttctgttttg aggtagttct caatctgctc aatgtctgca agaacattca
      721 cacttgcact tctggaatca gcaatttcag cctttgaacg acctgatgaa aaaggatatg
      781 cctcgacacg taaaaataga taaacatatt cgttggcctg tgaatcaaag tgggttatat
      841 tttctggata caggtatgtt acccggatat gtcctgcctg aaaaggaatg ttcatgctat
      901 tgccataggc tgcagatata tgggccagga ctggtttttc catgcttttg aaaatggaac
      961 tgttgagaac atcaagcttt ttcttgctac tcattaagtc aggagtgtaa agtgtcttaa
     1021 tggcaaccaa actagtaaca ggtgtttcac gaagtccgtt gatatagact tgctgatcca
     1081 cgtttatttg tacaatctgc cgttgaattt ctgctatctc attttcaatc ttggcgattg
     1141 ctgttgcgta attttcaatt cgtatggctc gatcgttttg acctgatttc agttgggctc
     1201 tttcctgaaa tgcttttcga taatcttgtt ccagtttttg catctgttca tgccattttg
     1261 tttctgaaac caaaattgtt gccaaccatt gtgtttcaac tgcgggaatt gaataaaaat
     1321 cctgaatgcc tttattatta actatgtgtc cgattacagc atcgtgaaaa acttttagat
     1381 tgtcacctga aggtacctta tgaaaattgt aattgatatt agagtcatct gttgatggac
     1441 tagaatttgt gtttccagaa gttgactcct gagcgcctcc tgatagtgca aacaagaaca
     1501 atgtaaagaa aatagctgtt aatatacgaa agtagttcat ttgttttacc atataaataa
     1561 tgttaaaaat gaaaagtggt tctgactata catatttgca tctatatcga cgtaagacat
     1621 tatggttgta cagtgtaata attcaagtca atattttgtt ttgtctcatc tgaattttgt
     1681 tttttatcga atattctgac tttattaata gaatactgat gtaaaaatca tgagtcaaga
     1741 ctggttttac gatcttcaag tcagattttg gtgaggtaga aaaatataaa tattccacga
     1801 taaaaacaaa aatgtgaatc aaaaattcag acagatcatt tttgaaagcc aaacacattg
     1861 atttacaggt aatattttgt tataatccag tacagaaata ttaaaaagga taaaaatgcc
     1921 actatatgat tatcagtgtc agcaatgtac gaatggtttt acagaacttc gtcggagttc
     1981 tgaaatggat actcaaattg attgtcctga atgcggcagc agggaaacaa tacgttctat
     2041 cagttctttt gcagttggcg gatcagtgtc tggaattcct tccgtctcag caccatcaag
     2101 aagcccattc agttgagcaa gctgctgaac ggactggtcg gtccgtataa aagtgcttga
     2161 ttgagagtga ctggtattca tccatctctc tctttgttag ctcgaggatg gaaaatatac
     2221 gctataagcg aaaagtagaa cgtctcgaaa tacatctttc ctgcataatt aaaacgtctc
     2281 agtatttatt tcaaaccttt ttccaaccta aataattatg gatgccagta ttcgtccgaa
     2341 aggtatcctc gatttccttt ctcagcaaga aatccgacaa ctcagtgatc cgcaaaacgg
     2401 aggacttaat gaattgttcc ggcgttgtgc actggctgtt cttaacagtg acagtcagac
     2461 agacagcgga aaagaaatgc tcgcgggttt tccggatttc agtatcgagg ttgtccagca
     2521 aacaagaagt atcaagctgg aattgagaaa tgcaccacct aaggcctttg taaatggcga
     2581 actgattcag ggaataaatg aacatttatt ttcagtttta cgagatattg tttttgtggg
     2641 cacagagctc tcaaataaaa gaggatttga tttaaaacaa tcttcttcta tcacagattt
     2701 tgtttatcat atattgagga atacaggctt gctcagagac ctgattgaac caaatctagt
     2761 ggtgtgctgg ggagggcatt caattgagga aacagagtat cagtatacaa aaaaagttgg
     2821 ttaccaactg ggattgcgtg agttgaatat ctgcacaggt tgcggtgcag gcgccatgaa
     2881 aggtccaatg aaaggagcca ccattgccca tgcaaaacag cgatacaata acgggctcta
     2941 tatcggcata actgaacctg gcattattgc ttcagaatca ccaaatccta tcgtaaactc
     3001 gttggtggtt atgcctgata tagaaaaacg acttgaggct tttgttcgta ccggacacgg
     3061 tttcattgtg tttcccggag ccgttggaac gactgaggaa atcttatatt tactcggtat
     3121 tctgatgcat cccaaaaatg ctgagcagcc cttccccctg attttaacag gtcccaaaga
     3181 aagtaaagat tattttgatc aaattgacgg ttttatcggt gcgactttgg gggcagaggc
     3241 ccagagattt tatgaaatta ttatcgatga tcctgaaaaa gttgcacaaa gaattcaatc
     3301 aggcatcagg aaagttcgtg aatttcgtag aaagttggga gatgcctttt attacaactg
     3361 gatgttacat attgatcaag attatcagtg gccgtttagt ccaagtcatg cagcaatgtc
     3421 tgcattaaat ttgcattatg cacagccaaa acatgaactt gcctgtaatt tgcgtaaaat
     3481 gttttctgga atagtatcag gtaatatcaa ggaagaaggt gtactttcca tcgaaaacaa
     3541 tggtccgttt caaattagcg gagatccgga actaatgcag gaactggata aactgctttc
     3601 gacattctgc agtcagcgga ggatgaaact ttccagagac acagaatata aaccatgcta
     3661 cgagatagtt gcttgataaa agaactgaca tctgttgctg atgcaaatca atccttcgag
     3721 tggggttcag gtaaagaggc cgactcaatt cgcactttaa cttcccagga aatggttgtt
     3781 tcagttcaga atattttgaa agggcacaat ttggaagaag gagtttgggt ttttggatat
     3841 ggctcattga tgtggaatcc ggactttaaa gttgcagaaa aaatttcagg gataatatct
     3901 ggctatcata gacgtttatg tttaaaatca atagtttaca ggggaactcc ggattatcac
     3961 ggtttggtat tcggtctgga taagggagga tcctgtcagg gaatggcctt ccggattgct
     4021 cctgaaaact tagactctga actgcagaaa gtctgggaac gtgaaatgtt tgcagaaact
     4081 tatattccta cctgggtttg tgtaaaaaca gaaaatggtg atgtttctgc cattactttt
     4141 gttatcaaca ccaaacacga acattatgtg ccggatctcg atctggaaaa agttgcagaa
     4201 cgagttgttc ttgcagaagg aaaatgcggt tcctgccatg attatgtgca aaatacagta
     4261 aaatttctgc atcaacttga actacgtgat cctgttttag agcagttgct gaccttaatc
     4321 gaatatcctc aaatttctgt ttgaagtaac taatacttct tgttatgaac aaacctattt
     4381 caaaaaggcg aatctcagta tggttgaaac tgattatcgc atttatactt ttaggagtcg
     4441 gttttgcagg aggtttcaag ttgtatgaga aaggcaacga ggcaggatgt ttccctctgg
     4501 aagatgatat cgtgccagta gaaataatac cttttcgtgc agatcatctt caggtaattg
     4561 tacttgggga cacagggaca gggaacgaag atcaactgaa agtagctgat ggaatggcta
     4621 aagtctgtga tcaggccgga tgtgattttg tcttactcct gggggacaat ttttatccga
     4681 atggtgtgaa atcaatactt gacaaacagt tttacacaaa atttgagcag gtttacaata
     4741 aaataaagaa gccattcttt gcagttttgg gaaatcacga tgtcaagcag aacgctttca
     4801 cgcaaataat gtatagtttg agaagcgatt attggcgaat gccaaactac gagtattcat
     4861 ttgagactgc ccaagcccgg ttctacggct taaacacgaa ctgtcccttt tcttttgaaa
     4921 gactcaggaa taaactgaac cgggatgatg tggagttaaa ggcaaatgcc gataaacttc
     4981 cctggacaat agcctttgga catcattcag tctattcaaa tggaacacac ggtgatactg
     5041 atgttattac acgcaattac tggaactgga tattggatgg gcggatagat ctatatttag
     5101 caggacataa tcatcattta tcactattgc aatatggaga aacgtcgacg gaatatgtga
     5161 taagcggggc tggtggcgca cactatcgaa caaatagtga aagagaaaaa cttaataaat
     5221 cagaggcatc gagcgtattt acatataacg acacaggatt cgtctggctc gacatacgca
     5281 gtgaaaaact tttgatccgc ttccacgaca gcacgggtaa tatcctatac gaatttacaa
     5341 aaacgaggta gattttactg taaatctccg caagctcttt cttcccgagt aataatgtcc
     5401 attattcaat tcagtcacgc aaacggtttt cccgccaaaa catattcagt tttatttaaa
     5461 catctggaag gacataaaat atcagcgatt aacattttag ctgaaaatcg aaaaccgtca
     5521 gaaatcaatt ggcatgatat gacagaagat atattgaagt gtgctgggca atttgaagag
     5581 ccggttgttg gcgtaggaca ctccttggga ggtgtgttga ctttaatggc agctgctaaa
     5641 aaacctgcat tatttcagaa tattatcata ctggaccctc cactattttc ttctcaaaaa
     5701 cgattactga tcagcatact gcgtgccata aatattgaag actgggtaag tccgtctgga
     5761 aaatcaaaaa atcgccgtag tcattttgaa tcaaaggaaa aagcccatag atatttccag
     5821 gcgaataaaa tattcagtaa ttttcatcca cagacattga aagattatgt aacccacggt
     5881 ttgatgctgg gaaaacaggg ggtagaattg acgattacag tggagcaaga ggttgctata
     5941 tttcacaaaa tgttgacatc ttatcctaaa tcaatataca aagtacgggg aacacttgtt
     6001 tatgcggtca aaaacccagt tctttgggca tcagatttgc gctggattaa aagaaatttc
     6061 acacaattga agctcattcc atttccggat tcacatttgt ttcctctgaa ttatcccgag
     6121 tcaactgctc aaattattaa cagatgctta aagtgtttaa attga
//
LOCUS       DTVD01000194            1508 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_40088_length_1508_cov_250.286304, whole genome
            shotgun sequence.
ACCESSION   DTVD01000194 DTVD01000000
VERSION     DTVD01000194.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40088_length_1508_cov_250.286304"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="EYO68_06690"
     CDS             complement(<1..692)
                     /locus_tag="EYO68_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HIC07527.1"
                     /translation="MIPESFDYQRAGSVSEAISLLQQGGEETKILAGGHSLIPTMKLR
                     LSTPETLIDIGGIPELKYINDKGDYLAIGAGTTHWAIESSDLVSQKAPALSHAAGQIG
                     DVQVRNRGTIGGVLAHSDPQADYPGVVLALDATIVVQGSGGERTIPVSDYFTGLWETA
                     LGEGEILTEVRIPTDSANANSCYLKFPQPASRYPYVGCAVAMSTSGGNCSDVRVGFSG
                     VGECGFRDSGVE"
     gene            complement(720..>1508)
                     /locus_tag="EYO68_06695"
     CDS             complement(720..>1508)
                     /locus_tag="EYO68_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC07528.1"
                     /translation="IGMDDVNVIHGDTENVPFGMGTYGSRSLAVCGSAIMKSVDKVKE
                     KGARIAAHKLECSPKDLEFANGSWNVKGTEKSVGFGDVALTAYVPHDYPENEEPGLDF
                     ASFYDPVNFVYPFGAHICVVEVDKETGEVKIVRYIAVDDAGNIINPMIVEGQVHGGVA
                     HGIGQALYEGAVYDDSGQLLNASMMDYCIPRADNMPFFETDHTVTPCPHNPLGVKGIG
                     EAGTIGSTPAVVNAVIDALSDYGVKDLQMPLLPQRVWAAMQQAK"
BASE COUNT          398 a          392 c          305 g          413 t
ORIGIN      
        1 tcttcaactc cagaatcccg gaaaccgcat tcgccaacac cgctaaatcc aacacgaaca
       61 tctgagcaat ttcctccgga agtactcatg gctaccgcac aacctacata aggataacga
      121 gatgccggtt gtggaaattt cagatagcat gagtttgcat tcgcgctgtc cgtgggaata
      181 cgtacttcag tcaggatttc tccctcgccc aaagccgttt cccaaagtcc ggtgaaataa
      241 tcggagaccg gaatagttcg ctctcctccg gaaccttgga ccacgatcgt ggcatcaagt
      301 gccagcacta ctcccggata atctgcttgc ggatcggaat gcgctaaaac gccgccgatg
      361 gtcccgcgat tccggacttg cacatcgccg atttgtccgg cggcgtgact caaggccgga
      421 gctttttgtg acaccaaatc agacgattca atcgcccaat gcgtggttcc ggctccgatt
      481 gccaaataat cgcctttgtc gtttatgtat tttaattccg gaattcctcc aatgtcaatc
      541 agggtttccg gagttgacaa gcgcaatttc atggtcggaa tcaaactgtg tcctcccgct
      601 aaaattttgg tttcctcgcc gccttgttgc agaagcgaaa ttgcctcact caccgagccg
      661 gcgcgttgat aatcaaaaga ttctggtatc ataataacct tttatcaata gggttagatt
      721 tatttcgctt gttgcattgc agcccaaaca cgttgcggca aaagtggcat ctgaagatcc
      781 ttcacgccgt agtcagacaa agcatcaatt acagcattga cgacggctgg agtggaaccg
      841 atggtcccag cttcaccgat acctttaact cccaatggat tatgagggca aggtgttact
      901 gtatgatctg tttcaaagaa tggcatgttg tcagctctcg gtatgcaata atccatcata
      961 gaagcattta acaattgacc agagtcatca tagactgccc cttcgtaaag ggcctggcct
     1021 atgccgtgtg ctacgcctcc atgaacttgt ccttcaacaa tcatgggatt tataatatta
     1081 cctgcgtcat cgactgcaat atacctgacg attttaactt ctccagtttc tttatctact
     1141 tccaccacgc atatatgtgc accgaatgga taaacaaaat ttactggatc gtaaaaactt
     1201 gcgaaatcga gaccaggttc ttcattttca ggataatcgt gaggtacata tgccgtaaga
     1261 gcaacatcac caaaaccgac agatttttct gtacctttta cattccacga tccattagcg
     1321 aattcaaggt cttttggaga gcattccagt ttatgagcag caatccgagc acctttctct
     1381 tttactttgt ccacactttt cataatggca ctaccacaaa cagctaggct cctagaacca
     1441 tatgttccca ttccaaatgg tacattttct gtgtcaccgt ggattacatt gacgtcatcc
     1501 ataccaat
//
LOCUS       DTVD01000195            6120 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4055_length_6120_cov_318.060511, whole genome
            shotgun sequence.
ACCESSION   DTVD01000195 DTVD01000000
VERSION     DTVD01000195.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_6120_cov_318.060511"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="EYO68_06700"
     CDS             <1..171
                     /locus_tag="EYO68_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020169637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07529.1"
                     /translation="LQRNEGKTIIMVEQNAKKGLEFCDIGYVLVSGNLALAGSGNELL
                     ENPEVGRLFLGG"
     gene            complement(401..1420)
                     /gene="pheS"
                     /locus_tag="EYO68_06705"
     CDS             complement(401..1420)
                     /gene="pheS"
                     /locus_tag="EYO68_06705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIC07530.1"
                     /translation="MLEQINMLREKGQNELNAITSTDQLPEWYHQYLGRKGDLTALLK
                     QLGTLSSEERPVMGKVINEVKQQLQESFELRQKTLKETKLHQELEIDDLDVSLPGRPC
                     TTGHLHLTTQTLRSIYNIFQEMGFQVYEAPEVESDEYNFQLLNIPEYHPARDMWDTFW
                     VNDEVVLRTHTSPGQIWAMREYYPEPIRVILPGKCYRFEQITPRSEHQFYQVEGLTIG
                     KNIRLTDLIGVMSEFAHKMYGAERKVRIRGSYFPFTEPSIEIDMSCSCEKKGCRLCKY
                     TGWLEVAGAGMVHPVVLRNGGYDPEEWSGFAFGMGVERPAMLKHNINDIRRFYSNDLR
                     FLQQF"
     gene            complement(1442..2350)
                     /locus_tag="EYO68_06710"
     CDS             complement(1442..2350)
                     /locus_tag="EYO68_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07531.1"
                     /translation="MSSIIDALEERLLQFLLEQETKKTWEDWLQWLADLDCPLEFEKP
                     LQIPNRPFSFQTWLNACDQQLNQHPDWQARLTTPVWLKRQQITVIPKVLKGGENKTPG
                     ITEISGLQTELTRMIDQIHTVQQRIANLETGSAQRSESPAAQTASKLIPATPEVPAAL
                     SVSGLSDPLGRPDLARTLERELQMHSKIPNAKGSAGTKSETDIPEPEIVKLKDAYLSY
                     NRWCDQFDEVHLDLQEFVKETLRVASSHQISFQQFRYRILKNAKEVKQLESVLESFSR
                     LRSSGIDINLAETESTRESSEADSNS"
     gene            complement(2376..3152)
                     /gene="lpxA"
                     /locus_tag="EYO68_06715"
     CDS             complement(2376..3152)
                     /gene="lpxA"
                     /locus_tag="EYO68_06715"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIC07532.1"
                     /translation="MNKIHPTAIIEDGAELGTDVEIGPYCMVGSGVKIGNGCSLKSHV
                     VLDGQTTIGTENIFHPFSYVGGEPQDLKYNQEKSLVIIGSKNIFREGVSVHRGTVGGG
                     GKTIIGDDNLLMGYVHVAHDCSIGNHNILANYTGLSGHVTLDNYVTLGGQNGVAQFLR
                     VGSYVYTGAASLIDRNIPPYATGYGNRFEVKGVNIVGLKRQNFSRESIGEILEAHRIY
                     YRPDLKNDEALRVIEETFGESAAVRAFLDFIYSAEGSGKH"
     gene            3384..3992
                     /locus_tag="EYO68_06720"
     CDS             3384..3992
                     /locus_tag="EYO68_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07533.1"
                     /translation="MIKYTFILLSTLLFAFTASAETLRLNSGESLEGKILKMDENTLF
                     IESHLTAQELKIDRADLSLIEFDSSARSLARRLGIGLHYRPNGNEENLSIKNWISAVD
                     SAELIVGYSSGTQDTFGFELRYSRVFMVEGATDLFYGAGAGILSIDSKRGTIFRLYSG
                     SEFFPLSSPSFGFSLELGVQRIQGVGDVTQSFYNALAARYYF"
     gene            4004..4456
                     /locus_tag="EYO68_06725"
     CDS             4004..4456
                     /locus_tag="EYO68_06725"
                     /inference="COORDINATES: protein motif:HMM:PF01713.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07534.1"
                     /translation="MGKKSSLPSRLTFEESEQNDALMEKIFAGLDDVPDKEPALEIPP
                     SESSLKKPRTSPADSVSSPDAELDLHGKTREEAIMMVQNFVMTCHANQFRTALIITGK
                     GHHSGNQGPVLKREVQLWLERNGKQYISDFHTAPARFGGSGAIWLNIK"
     gene            complement(4463..4846)
                     /gene="apaG"
                     /locus_tag="EYO68_06730"
     CDS             complement(4463..4846)
                     /gene="apaG"
                     /locus_tag="EYO68_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIC07535.1"
                     /translation="MEEAVTEGVQVQVESFYLDSHSIPEEDLYVFAYRIRLKNLSQRT
                     VQLLRRHWIITDSNGDINEVRGDGVVGDQPVLVPDEEYEYTSGSQLKSPVGTMQGTYR
                     MTTTDGDEFDIAIPCFTLAVPGMIN"
     gene            5053..>6120
                     /gene="obgE"
                     /locus_tag="EYO68_06735"
     CDS             5053..>6120
                     /gene="obgE"
                     /locus_tag="EYO68_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIC07536.1"
                     /translation="MKFVDSVKIHVHSGNGGGGCTAFRREKYIPHGGPDGGDGGKGGD
                     IFMFGDSGKNTLLDLSFQQHQHAEHGISGGGSDKHGRNGKDLHIRVPLGTVVKDIETG
                     EIFLEVVEEKDYLLFAGGRGGRGNARFKTSTNRSPEYHQPGEPGHECWVRLELKLMAD
                     VGLVGFPNAGKSTLISSVSQAHPKIADYPFTTLVPQLGVVKINNLDPFVIADIPGIIA
                     GAHDGRGLGDQFLRHIERTASLLLLLDVSGFSENPPQEEYQTLIEELELFSPKLLEKS
                     RAVAFTKLDSVSDFEPLDELQQHLEASGETVFRVSSVSGDGIQELLSYLGSVVQKERQ
                     RENEKPPNIVEEALPENSIWDD"
BASE COUNT         1675 a         1351 c         1345 g         1749 t
ORIGIN      
        1 cttcaacgaa atgaaggcaa gaccattatc atggtggaac agaatgccaa gaaaggtctt
       61 gaattctgtg atattggcta tgtactggtt tccggtaact tggccctggc tggaagcggg
      121 aatgagttgt tggaaaatcc agaagttgga cgtttatttc tgggaggata aaaaagtttc
      181 agtagttcag tgttgctttt cagaatttcc ttctacaaga tatatcaaag atatttcccg
      241 gaggtagaag cctcctcggc cgggagagga gtgccgtaag gttgctgctg ctgtccaagc
      301 tgcaaatcat gtgttcccac gtaagccagt cccaataaca gcaatgctat aaaaatcaga
      361 atcaccttct tttcctgcgg atcaaagtta tcaagcataa tcagaactgc tgcaggaatc
      421 tcaggtcgtt gctgtaaaag cggcgtatat cgttaatgtt atgtttgagc attgcaggcc
      481 gttctacgcc catgccaaac gcaaatccac tccattcttc aggatcgtaa ccaccgttgc
      541 gcaaaacaac cggatgtacc attcccgcac cggcaacttc cagccagcct gtatatttgc
      601 agagtcggca gcccttcttt tcacaactgc agctcatgtc gatttcaata ctgggttctg
      661 tgaaagggaa ataactcccc cgaatacgaa cttttcgttc tgcaccgtac attttatgcg
      721 cgaattcact catcacgccg atcaggtctg tgagacggat attttttccg atggttaaac
      781 cttcaacctg ataaaactgg tgttcagaac gtggagtgat ttgttcaaaa cggtagcatt
      841 tccccggcag aatcactcga atcggttccg gataatattc acgcattgcc caaatttgac
      901 ccggtgaagt gtgggtacgc aaaacgactt catcgttgac ccagaaagtg tcccacatat
      961 cacgcgcagg atgatattcc ggtatgttca gtaactggaa attatactcg tcactttcca
     1021 cttccggtgc ttcgtaaacc tgaaaaccca tttcctggaa aatattgtaa atactacgta
     1081 aagtttgagt ggtcaggtgg aggtgaccag tggtacaagg tcttccggga aggctaacat
     1141 ccaggtcatc aatttccagt tcctgatgaa gttttgtttc cttcaaagtt ttctggcgca
     1201 gttcaaaaga ttcctgtaac tgctgcttga cttcgttgat gacttttccc atgacaggcc
     1261 tctcttctga agaaagagtc ccaagctgtt tgagcagggc agtgaggtca cctttgcgtc
     1321 ccagatactg atggtaccac tcaggaagtt gatctgtact ggttatagca ttaagctcgt
     1381 tttgcccctt ttcgcggagc atatttattt gttcaagcat tttataatta cagattattt
     1441 attaggagtt tgaatccgct tccgaacttt ccctggtgct ttctgtttca gctaaattta
     1501 tgtcaattcc tgagctgcgc agtcgtgaaa aagactccag gacactttcc agttgtttaa
     1561 cttcttttgc atttttcaga atgcggtacc tgaactgctg aaaggaaatt tgatgactgc
     1621 ttgcaactct gagagtttct ttgacgaact cctgcagatc taggtgcacc tcgtcaaatt
     1681 gatcgcacca gcgattataa cttagatatg cgtctttaag tttcacaatc tcaggttcag
     1741 gtatatccgt ttcagatttg gtgcctgccg aacctttcgc atttggtatt ttactgtgca
     1801 tctgcaactc cctctccagt gttctcgcaa gatcaggacg acccagagga tcagataaac
     1861 ctgaaacaga taaagctgcc ggcacttcag gtgtcgctgg aatcaactta gacgcagttt
     1921 gtgctgcagg gctttctgac ctttgcgcgg aacctgtttc caagttggct atacgctgct
     1981 gaaccgtatg tatctgatca atcatcctgg tcagttctgt ctgcagacca ctaatctcag
     2041 taattccggg agttttattt tctccacctt taagcacttt ggggattact gttatttgtt
     2101 gtctttttag ccagacaggt gtggtcagtc gcgcttgcca gtctggatgc tgattcagct
     2161 gttgatcgca agcattcagc catgtttgaa aactgaatgg ccggttagga atctgcagcg
     2221 gtttctcaaa ttcaagcgga caatcaaggt cagctaacca ctggagccaa tcttcccacg
     2281 tcttttttgt ttcctgctcc agcaggaact gcaaaagtcg ctcttcaagc gcatcaatga
     2341 tgcttgacat tcgaactctc tattaaaaac tagaatcagt gcttaccgct tccttcagca
     2401 gaataaatga agtccagaaa cgctcgaact gcggcacttt ctccaaatgt ttcctctatt
     2461 acccgcagag cctcatcatt ttttagatcg ggacgataat aaatcctatg ggcttcaagt
     2521 atttctccaa ttgattcacg gctaaaattc tgacgtttca agccgactat attaacaccc
     2581 tttacttcaa aacggttccc ataacctgtg gcataaggtg gaatgtttcg atcaatcaaa
     2641 gaagcagctc ccgtataaac ataagatcca actcttaaaa attgagctac tccgttctgt
     2701 ccgcccagag tgacatagtt atcaagagta acatgaccgc tcaaaccagt gtaatttgca
     2761 agaatattat ggttgccgat gctgcagtca tgtgcgacgt gtacgtaacc catcagcaaa
     2821 ttatcatctc ctatgattgt ttttccgcca ccgccgacag ttccgcgatg tacagaaaca
     2881 ccttcacgaa aaatgttttt gcttccgata attaccagtg atttttcctg attatatttt
     2941 aaatcctgag gttcaccgcc tacataagaa aaagggtgaa aaatgttttc agttccaatc
     3001 gttgtctgac cgtccaggac aacatgggat tttaaactgc agccatttcc aattttgaca
     3061 ccggaaccaa ccatgcagta gggcccgatt tcaacatcag tgccaagctc tgcaccatct
     3121 tctatgatgg cggttggatg aattttattc ataaaaaatt attagtcatg tgcgttgtga
     3181 aggctctttt gctcctgcaa agtgataaat gaatacatcc attgtgtgct aagttaaaac
     3241 ctaaagtcaa gaaaacactg tggagaaaga aacccactct tttctgaaac attttctgta
     3301 gatgttcact gaagcaaggt tcacgaattg cattcaaccc tcataatgaa gacattattg
     3361 aaaacgacaa acgaatggaa actgtgataa aatatacatt tatattgttg agtacactgc
     3421 tctttgcctt tactgcaagt gcagaaactt tgcggctaaa cagcggagag tctctggaag
     3481 gaaaaatcct gaaaatggat gaaaatactt tgtttattga atcacatctt actgcccagg
     3541 aattaaagat tgaccgggcg gacctgagtc ttatcgaatt tgactcatcc gcccgcagtc
     3601 ttgcccggcg gcttggaata ggccttcatt acagaccaaa tggaaatgag gagaaccttt
     3661 ctataaaaaa ctggattagt gcagttgatt cagcagaact gattgttggt tacagttcag
     3721 gcacgcagga cacatttgga ttcgaattac gttattcccg ggtattcatg gttgaaggcg
     3781 ccaccgatct tttttatgga gccggtgcag gaattctttc catagacagc aaacgcggca
     3841 caatatttag actttattct ggcagtgagt ttttccctct gagcagccca agttttggtt
     3901 tttcactgga attaggtgta caacgtatac agggtgttgg agacgttact caaagctttt
     3961 acaatgcatt agctgcacgt tactatttct gaaagcggca aacatgggca agaaaagctc
     4021 gctcccgtcc cgtctcactt tcgaggaatc tgagcagaat gatgctctaa tggaaaagat
     4081 ttttgccggc ctcgatgatg tcccggataa agagcccgca ctggaaattc caccctctga
     4141 atcttccctt aaaaaacccc ggacctctcc agcagactca gtatccagtc cggatgctga
     4201 gttggactta catggcaaaa cccgtgaaga agcaattatg atggtgcaga attttgtaat
     4261 gacctgccat gcgaatcaat tccgtacagc acttatcatt accggaaaag gtcaccattc
     4321 agggaaccag ggtcctgttc tgaagagaga agtacagctt tggctggagc gtaatgggaa
     4381 acagtatatc agtgattttc atacagctcc tgcgcgtttt ggaggttccg gtgcaatttg
     4441 gctgaatatc aaataattcg tgtcaattta tcattcctgg aacggctaga gtgaagcatg
     4501 gaattgcaat atcaaattcg tcaccgtctg ttgttgtcat ccgatatgtc ccctgcatcg
     4561 ttccaacagg tgatttaagc tgggatccac tggtatattc atattcctca tccggaacca
     4621 aaaccggttg atcccccacc acaccatcgc cccgtacctc attaatgtct ccattgctgt
     4681 cggtgatgat ccagtgtcgc cgtagaagct gcacagttct ctggctcaag tttttcaagc
     4741 ggatacggta ggcaaagaca tagagatctt cttcagggat tgaatgagaa tcaaggtaaa
     4801 atgattcaac ctgaacctga actccttcgg taactgcttc ttccattatt ttcaaatttt
     4861 ctgcaaaaaa tatactattc aaaatttagt tcgtgttata cagtaattcg agagaatggc
     4921 aaagtgaaaa atatagaagt aattggaatt ataagcatga cttgcccttc aaccgtactt
     4981 tgagtaaaat attttcctgt gcaagcttct aaattctatc tcaaaaagct ctcgcttaca
     5041 ctcgtatatt tcatgaaatt tgttgattca gtaaaaatcc atgtccattc cggaaatggg
     5101 gggggcggct gtacagcctt ccgaagggaa aaatatattc cgcacggagg acctgacggt
     5161 ggtgacggcg ggaaaggagg tgacattttc atgtttggag attcaggaaa aaatactctg
     5221 cttgatcttt cttttcagca gcatcaacat gcagaacatg gaataagcgg cggcggcagc
     5281 gacaaacacg gacgtaacgg gaaagatctt cacattcgtg ttccgcttgg gacagtggtc
     5341 aaagatattg aaactggtga gatatttctg gaggtagttg aagaaaaaga ctatctgcta
     5401 tttgctggcg gacgcggcgg acgcggcaat gcacgcttca aaacttcaac caaccgctct
     5461 ccggagtatc accagccggg agaaccgggg catgaatgtt gggtccggct ggaactgaaa
     5521 ttgatggcag atgtcggttt ggttggtttc ccgaatgcag gaaaatcaac tctgatcagt
     5581 agtgtgtctc aggcacaccc aaaaatcgcg gattatcctt ttactacgct ggttccgcag
     5641 cttggtgttg tgaaaataaa taatctggat ccatttgtga ttgccgatat tccgggcata
     5701 attgcaggcg cacacgacgg cagaggactg ggtgatcagt ttttacgcca catcgagcgt
     5761 actgcgagtt tattgctgct gctggatgtt tcaggatttt ccgaaaatcc acctcaggaa
     5821 gaataccaga ctttgattga agagctggag ctgttttcac caaaattatt agaaaaatcc
     5881 cgtgcagttg catttacaaa actggacagt gtcagtgatt ttgaaccact ggatgagtta
     5941 cagcagcatc ttgaagcttc cggagaaact gtctttcgtg tctcttcagt aagcggtgat
     6001 ggaattcagg agttgctgag ttatcttggt tcagtggtac aaaaggagcg tcagcgggag
     6061 aatgagaaac caccaaatat tgtagaagaa gcacttcctg aaaactcaat ttgggatgat
//
LOCUS       DTVD01000196            6080 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4088_length_6080_cov_335.800000, whole genome
            shotgun sequence.
ACCESSION   DTVD01000196 DTVD01000000
VERSION     DTVD01000196.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_6080_cov_335.800000"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(174..1079)
                     /locus_tag="EYO68_06740"
     CDS             complement(174..1079)
                     /locus_tag="EYO68_06740"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIC07537.1"
                     /translation="MKTAANTIPRNAPCWCGSGKKYKKCHMEKGQKQVPLPETKPKPK
                     GRQVIIKTEKQIEGIRKSSQLTKILLDMVEERIGEGVTTNEIDGWVYRETLANGAIPA
                     PLNYGHGQGRRRMPFPKSVCSSPNNVICHGIPNNQALQSGDIVNIDVTCDLDGYFGDA
                     SRMFIIGDVPEKTRELVEVTRECLELGIEQVCPSNTLGDIGHAIQQHAEKHGFSVVRD
                     FAGHGVGVEFHEPPQVLHYGQPGTGVTIQENMIFTIEPMINMGSYECKVLGDGWTAVT
                     VDGELSAQWEHTLLVTESGVEVLTA"
     gene            complement(1113..1802)
                     /gene="lepB"
                     /locus_tag="EYO68_06745"
     CDS             complement(1113..1802)
                     /gene="lepB"
                     /locus_tag="EYO68_06745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIC07538.1"
                     /translation="MKNEADLLKAAKGFLRLDLLIPNRTIREWAEALIFAVVVATIVR
                     TYLFAPYQIPSGSMLPTIQIGDHIFASMYSYGSPIPFTDIKLFKKPVLRGDIIIFPFP
                     SDPSVDYIKRAVGLPGETLEIRKDQVFINGEQLDEPYAYYEPNERRTRLAQGLMSSTP
                     VSRFGPIKIPEGKIFAMGDNRYNSADSRYWGFVDLKTVAGKGQIIYWSHDPDENIFGG
                     YRFGRLLDYLK"
     gene            complement(2186..3253)
                     /locus_tag="EYO68_06750"
     CDS             complement(2186..3253)
                     /locus_tag="EYO68_06750"
                     /inference="COORDINATES: protein motif:HMM:PF13641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIC07539.1"
                     /translation="MGYIKKKVELESSPQSKQKAEVLSIVIPAFNSAKLLSICLEALE
                     KQTAQKSCFEVTVADDGSTDETLQMLQQFKARSELQLQWTTIPNSGPGNARNAGVALS
                     SGTWIGFMDADVIPHPDWVKNALTLIQQKPEAGAFEGRTEVTQRSRATPFTHQTENID
                     GGRYPTCNFLVRRNLAHFHPAYLIPFREDTDLAFSILAAGFPIIFAPELIVEHPPLPS
                     SYLRPLVLARRYYYDGLLSRRFPRRYRNDLDAHQIIGLKIPHLKKKLYSLFVLSQIVF
                     LGSQLFGLSGKSLFLAGSAYLLCLLMTAGASLRFANLRDLSLKDWLVFICQQHVLPWV
                     MGYSLFRGWLDFRGEPEYSAS"
     gene            3252..4556
                     /gene="argA"
                     /locus_tag="EYO68_06755"
     CDS             3252..4556
                     /gene="argA"
                     /locus_tag="EYO68_06755"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIC07540.1"
                     /translation="MPQHSLDPELSGFIHNLRASLPYLEEFHQQTFVVQLSGDLLNGH
                     NFRVIEDLALLQQVGIRIVLIHGAETQIRKFLTDGGLVCQTEDGILVAEETHLPLIEQ
                     AISSANWQLLTRFRSCSRQLQTLTGHFMFAEKKSFSGNFGTHFTGNVCGLDLETLRQE
                     ISNSKLVILPPFALGEKGRLWILDPNQVAFEVATRLRAKKFIVLDTLTLPQIDGSDSL
                     EMTTESVQQWLKKNPDLPSSQRLQLNVLTEACVHGVERCHLLDGRVEGALLAELLTPK
                     GAGVMITNSSYKRTRPAQLSDLQSILEILKSPVQHAAIVLRTSSYIEQQIDNYLVFCI
                     DEDVVGCCEVIYYPDGSAAEIASLAVNKSYRNQGIGSELIQAASEKIRSGNCKLAFAL
                     STATSHVFTQCGFREISPEELPEQKRKNYDFQESIVYGRNMD"
     gene            complement(4566..5204)
                     /locus_tag="EYO68_06760"
     CDS             complement(4566..5204)
                     /locus_tag="EYO68_06760"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07541.1"
                     /translation="MGQLIDEDDCEVCVPFDQEWIFTYGDLVTLLLCFFILLFSSCKY
                     ETMKFKSLAASFKPLPAGTPFLNEGKDSIVEQIAQQLEESELGDDANINVEEKGVVVS
                     FKASAYFKTDSAKLNAKAVKEMTRFSKLIFLLPNKIQIEGHTEKKQPDNWDSTWELAG
                     ARASKIARFFIENGLDPTKITVKSFGSTRPRFREASPTQRSLNNRVEVVVLP"
     gene            complement(5243..5899)
                     /locus_tag="EYO68_06765"
     CDS             complement(5243..5899)
                     /locus_tag="EYO68_06765"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07542.1"
                     /translation="MGQMIDEEDCEVCIPFEQDWIFTYGDLVTLLLCFFILLFSMCKT
                     DIEKSRQISESFKSMPPGSPFIFSGQSSNLDKASKELEQLDVPDDVSINASKAGLEVT
                     FSKTVAFEQGSVSISEKAKKTLIKMLPIIGQLQNNIEISGHTDESDSNQKYPSNWELS
                     VARASVIAAFLESKLIPVDRIQVAGYGDSRPRFNPDTAYKRNLNRRVQILLLPEDQTR
                     "
BASE COUNT         1683 a         1379 c         1252 g         1766 t
ORIGIN      
        1 gcttaatgct cacatgcata caaaataatt gaaaacagta ttttgatgaa ctagtttaca
       61 aagtctataa ttcacaattg tttgtcattc ctgctttcgc gggaatggat aattttcagg
      121 taattgtcta atcttgattg ctaacaagca atacattgtt aatgcagaat gacttatgca
      181 gtcagtacct caactccact ttcagtaacc agcaaagtgt gttcccactg agcagataac
      241 tctccgtcca cagtcacagc cgtccagccg tcacccaaca ctttgcattc ataactgccc
      301 atgttgatca tcggttcaat tgtgaagatc atattttcct gaatggttac acctgtcccc
      361 ggctgaccgt aatgcagtac ctgtggaggc tcgtgaaatt ccactccaac tccatgtcct
      421 gcaaaatctc tgactaccga aaagccgtgt ttttccgcat gctgctgaat agcgtggcca
      481 atgtccccaa gcgtattaga tggacaaacc tgttctatcc ccagttcaag acattcccgt
      541 gtgacttcca ccaattcccg cgttttttct ggaacatcgc caatgataaa cattctgctg
      601 gcatctccaa aatagccgtc caaatcgcag gtaacatcaa tattcacaat atcaccgctc
      661 tgcagtgcct gattgtttgg aataccatgg caaatgacat tgttcggtga actgcagaca
      721 cttttaggaa acggcatcct ccgtcgacct tgaccgtgcc cgtaattcaa tggagccgga
      781 attgcgccgt tagccaaagt ttcacgataa acccacccgt ctatttcatt tgtggtcact
      841 ccctcaccga ttcgttcctc aaccatatcg agcagaattt ttgtaagttg gctgcttttg
      901 cggatacctt caatctgttt ctcggtttta ataataactt gacgcccttt aggcttaggt
      961 tttgtctcag gcaacggcac ctgtttttgc cctttttcca tgtggcattt tttatatttt
     1021 ttaccgcttc cgcaccagca gggtgcattt cggggaattg tatttgctgc tgttttcata
     1081 ctttcactaa aaaaactgct cttgctgact tattatttca ggtaatcaag taaacggcca
     1141 aatctatatc cgccaaaaat attttcatca ggatcgtgcg accagtagat aatctgtcct
     1201 tttccggcaa ctgttttcag atcaacaaaa ccccagtaac gactatctgc actgttgtat
     1261 cggttatctc ccatcgcaaa tattttacct tccggaatct taatcggacc aaagcggctg
     1321 accggagttg aggacattaa accctgcgcc agtcgggttc tccgttcatt tggttcataa
     1381 tatgcgtaag gctcatcaag ttgttctcca tttatgaaaa cctggtcttt ccgaatttcc
     1441 agtgtctcac caggcagccc aactgcgcgt tttatgtagt caaccgaagg gtcactcgga
     1501 aatggaaaaa ttataatatc tccccgcagt acaggttttt taaaaagttt gatatctgtg
     1561 aagggaatcg gtgagccata ggaatacata ctggcaaata tgtggtctcc aatctgaata
     1621 gtcggcagca tcgatccgga tgggatttga taaggtgcaa acaaataggt tcggacaatg
     1681 gttgccacta caaccgcaaa aatcagagcc tcggcccatt ccctgattgt gcggttcgga
     1741 atcagaaggt ccaggcgcaa aaaccccttt gctgctttca aaagatccgc ctcatttttc
     1801 atgatttctt ctgcctgagg gagcttccgg acacggctca aaaaaatata cgagtttgga
     1861 aataccctgt tctccagctc agtaaggtcg tttagagcaa catcactgac tccttttttc
     1921 cggagttttt taaatgattt ttctgaaagt ttataggtaa caaacatctt gaattacact
     1981 tattttctga tttaaatctt ttgctttact gacctgagtc agtttgtctt tgaatccaaa
     2041 ctgaataatt ccgggtatct gcattcaacg acaaattaaa atcagaccag gagttgacgt
     2101 ctggcacaac tcttcaaagg gtggtcttcc attctgaatt gctttgagta aatagtcaaa
     2161 ttatcgtata gtcctgccgt cacaattaac tggccgaata ctcaggttct ccgcgaaaat
     2221 ccaaccagcc ccgaaacagt gaataaccca tcacccacgg cagcacgtgc tgctggcaaa
     2281 taaagacaag ccagtctttg agcgaaagat cccgcaaatt tgcaaagcgc agacttgctc
     2341 cggcagtcat tagcaggcac aaaaggtagg cacttcctgc cagaaacaaa ctctttccgg
     2401 ataaaccgaa aagctgtgat ccaagaaaaa caatttgaga aagcacaaat aaagagtata
     2461 atttcttctt caaatgcgga attttgagac caattatctg atgtgcgtcc aaatcattac
     2521 ggtaacggcg gggaaatctc cgagacaata aaccgtcata ataatatctt cgtgctaaaa
     2581 ccagcggccg taaatagctt gaaggtagag gtggatgttc cacaatcagt tcgggagcaa
     2641 atataatcgg aaatcctgca gctaaaatgc tgaacgctaa atctgtgtcc tcccggaaag
     2701 gaataagata agccggatga aaatgtgcta agtttcggcg gaccagaaaa ttacatgttg
     2761 ggtagcgtcc gccgtcaatg ttttcggttt gatgcgtgaa tggcgtagcc cggctgcgtt
     2821 gagtgacttc tgtacggccc tcaaatgctc cggcttccgg tttttgctga attagcgtaa
     2881 gtgcattttt tacccagtcc ggatgtggaa taacatccgc gtccataaac ccgatccatg
     2941 ttccggaaga taaagcaact ccggcattac gggcattgcc tggaccggaa ttcggaattg
     3001 tagtccattg aagctgtaac tcagagcgtg ctttaaactg ctgcagcatt tgcaaagttt
     3061 catctgtgga accatcgtct gcaactgtaa cttcaaaaca ggatttttgc gcagtttgtt
     3121 tttcaagggc ttcaagacag attgacagca gttttgcgct gttgaaagca ggaatcacga
     3181 ttgaaagaac ttccgctttt tgtttacttt gcggtgaaga ttccagttca actttttttt
     3241 taatataacc catgccacag cattctcttg atccggagtt gtccggtttt attcataatt
     3301 tgcgggcttc cctgccctat ctggaagagt tccatcagca aactttcgtg gttcaactaa
     3361 gcggagattt gcttaatggt cacaatttca gagtaattga agatttagcg ctcttgcaac
     3421 aagttggtat cagaatcgtt ttgatccatg gcgcagaaac acagatccgc aagtttctaa
     3481 ctgatggtgg tcttgtttgc caaactgaag acggtattct tgttgcggaa gaaacacatt
     3541 taccgctgat tgaacaggca atttcttctg caaactggca gttactgact cggttcagga
     3601 gttgtagtcg gcagcttcaa accttaaccg gtcatttcat gtttgcagag aaaaaatctt
     3661 tttccggaaa tttcggtaca cacttcactg gaaacgtttg cggacttgac ctggaaacac
     3721 tccgtcagga aatttcaaac tcaaagcttg ttatactacc accttttgca ctgggagaaa
     3781 agggacgatt gtggattctt gatccaaatc aggttgcctt tgaagtggca acgcgcttac
     3841 gtgcaaaaaa atttattgta ctggatacat taacattgcc gcaaattgat ggctcagatt
     3901 ctctggaaat gacaacagaa agtgttcagc aatggttgaa aaaaaatccg gatttacctt
     3961 catcacagcg cctgcaattg aatgtactga ctgaagcctg tgttcatggt gttgaacggt
     4021 gtcatttgct ggacggcagg gttgagggtg ccctgctcgc agaactgctt actccaaaag
     4081 gagccggagt aatgattaca aacagttcat acaaacgcac tcgtcctgca cagcttagcg
     4141 atttgcagtc tattttggaa atcctcaaaa gtccggttca gcatgcagca attgttttac
     4201 gcacgtcttc ctacattgaa cagcaaattg ataattatct ggtgttttgt attgatgaag
     4261 acgttgttgg ttgctgcgaa gttatttatt atccagatgg ttctgctgca gaaattgcca
     4321 gtctggcagt gaataaatca tatcgtaatc aggggatcgg cagtgaactg atccaggctg
     4381 cttctgaaaa aatccgctct ggaaactgca aacttgcatt cgctttaagt actgccacct
     4441 cacatgtttt tactcaatgc ggatttcgtg aaataagtcc ggaagaactt ccggagcaaa
     4501 agcgtaaaaa ctacgacttt caggagtcaa ttgtttacgg aaggaatatg gattgattgt
     4561 gggtattacg gtaggacaac aacttctacc cggttgttta agcttctttg agtaggtgaa
     4621 gcttcgcgga agcgcggacg tgttgaacca aaactcttaa ctgtaatttt tgtgggatcc
     4681 agcccgtttt caataaagaa tcgagcaatc ttcgaggcac gtgctccagc cagttcccag
     4741 gttgaatccc aattatccgg ctgttttttt tccgtatgtc cttcgatttg aattttgttg
     4801 ggcaggagga agatgagttt ggagaagcgg gtcatttcct tgactgcctt tgcattcaac
     4861 ttggcagaat ctgttttaaa atatgcactt gctttgaaag agacaacaac ccccttttct
     4921 tcaacattga tatttgcgtc atctcccaac tcactttcct ccaactgctg ggcaatctgc
     4981 tcaacgatgg agtctttacc ctcattcaaa aacggagtac cagccggtag aggtttaaaa
     5041 ctcgcagcca ggcttttgaa tttcattgtt tcatatttgc acgatgaaaa gagcaagatg
     5101 aaaaagcaaa gcaggagcgt taccaaatca ccataagtaa aaatccactc ctgatcgaac
     5161 ggaacacaaa cttcgcaatc atcctcatca atgagttgtc ccattacagg tacgcagtat
     5221 taagttaagg gttgcagaga aactagcgtg tttgatcttc aggaaggagc agaatctgta
     5281 cacgtcggtt caagttacgt ttataagctg tatccggatt aaatcttgga cgggaatcac
     5341 catatccggc tacctggata cgatctactg gaataagttt tgattccaga aaagcggcaa
     5401 tcacgcttgc tcgtgctaca gaaagttccc agtttgatgg atatttctga tttgaatcag
     5461 attcatcggt gtgtccggat atttcaatat tgttctgcag ctgcccaata atcggcagca
     5521 tcttgatcaa agtctttttt gctttctcag aaattgatac agaaccttgt tcaaaggcca
     5581 ctgttttgct gaatgtaacc tccagtccag cctttgaggc attgattgac acatcatcag
     5641 gtacatccag ctgttcaagc tcctttgaag ctttatctaa atttgaagat tgtccactga
     5701 aaataaaggg actgcccggc ggcatgcttt tgaaactttc agaaatttgc ctgctttttt
     5761 cgatatcagt cttgcacatg gaaaagagca aaatgaaaaa gcaaagcagc agcgttacca
     5821 aatcaccata agtaaaaatc caatcctgct caaaaggaat acaaacttca caatcttcct
     5881 catcaatcat ctgacccatt atttactcct ttttcaggtt cagatcactc tccaccttca
     5941 gcggcggctt caccgccgcc ttcaaacttc aaagcttcat acattgtttt ttgaacgggc
     6001 ggcagatagt tcatcaagtc gttttcaata aaatccggac gctgtcctcc ctcaatataa
     6061 agtaccccgt cacggatcat
//
LOCUS       DTVD01000197            6076 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4094_length_6076_cov_235.709351, whole genome
            shotgun sequence.
ACCESSION   DTVD01000197 DTVD01000000
VERSION     DTVD01000197.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_6076_cov_235.709351"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="EYO68_06770"
     CDS             complement(<1..169)
                     /locus_tag="EYO68_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HIC07543.1"
                     /translation="MCGIAGIIRRGSPGNIGGEMTSMLQSLKHRGPDSTGFAVYGVPE
                     ENQFVMRFKVAE"
     gene            complement(220..1692)
                     /locus_tag="EYO68_06775"
     CDS             complement(220..1692)
                     /locus_tag="EYO68_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIC07544.1"
                     /translation="MTDLEAHVNAEGRDKLVKQVREKIDELGVTYIYYQFISVTGRIV
                     GKGIPADHWERTAERGFQLVYGSTANLYVDRHGDYIGYGPESSELVGIPDPETFSQLP
                     WDKRVARVFCTCFRNREERENPGGHLTSDCRGNLRIIHNEFQDKYDGLHLRCGTEPEM
                     MWLKRGEDGRPNGGVTKPNCYHIDQFEELRPTFMKVIEYSQAMGLDMIQGDHEDAPGQ
                     LELNTMFDDVLRNADRLTTYRQICAQVAREDGLIACFMSKPFMGVSASGCHHNMSLWR
                     GGKDTVNDLHNATLPGMAGAFTYLVGGDNTFMPDPSIDERKPGPIGLQCIGGVMKHLG
                     ALTSIGSSTVNSYRRLWDTGFWAPVFADWGYQNRTCALRISAPGRFEYRSVDSMVNPY
                     LMGAALLKAFDDGISNDLDPGEAEERNIYQAIEEGKQVKKLPMSLGEALQKLADDEVI
                     KSSMPDEMYRVFHHYKNDEWERFNHTVTEWDLETYIDCLP"
     gene            complement(1864..4215)
                     /locus_tag="EYO68_06780"
     CDS             complement(1864..4215)
                     /locus_tag="EYO68_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HIC07545.1"
                     /translation="MELTKVTTTSGILEPGLWQLDPSRERYRVPACGVIVVELYPDDV
                     LVVQDPEGGQHAEVVPFSPEGKGDPGILGINKSQPADGLRQILSGDSESAGRVRSGLE
                     RKGIDLASAKAAVLFAPDSLAREELRFQVTSRTTCAVAAPGTMMTVEGETLPPTDLQV
                     FIHRASPPEERETDLPDPLAEPRLDFQIDRCTSQAYEVKAGEFIQVIDVMGRECSDFQ
                     VFDHRKLDQGIERCLDVTTTRTLIGAGYPGPGLFSKYYDVDMQPLVEVIRDTVGRHDT
                     FGLACTAKSYEDRGYFGHINCSDNFNGALAQYEIEPRKGWAAANFFFNTGIDDHNVLY
                     GEESWSRPGDYVLLQAQTDLVCISSACPDDTTPVNGWNPTDIHVRVYPEKNTFSKAIA
                     IRMTPDADAKLTQETGFHPRTSALTRNFTEYRGYWLPTCYRNNGAIEEYHSCRENAIV
                     TDLSPLRKFEVIGPDAEALLQWTLTRNVRKLAVGQVVYSSMLYPHGGMMDDGTLLRLC
                     QDNFRWIGGDDYGGIWMREQAEKLGLKVRVKSSTDQIHNIAVQGPKSREILKEVVWTP
                     PTQPKLEEIGWFRFTIGRVGDLNGIPIMISRTGYTGELGYEVWCHPNDAPAVWDVIWE
                     AGQPHGMMPLGLDALDMVRIESGLVFAGYEFSDETDPFESGVGFTVPMKTKEDDFVGR
                     EALVSRKENPQRKLVGLELEGNEPGAHGDCVHIGRGQVGVITSGMRSPILRKNIALCR
                     IDVTHAEIGTEVQIGKLDGHQKRIPAQVVKFPFYDPEKLKPRS"
     gene            4860..>6076
                     /gene="glnT"
                     /locus_tag="EYO68_06785"
     CDS             4860..>6076
                     /gene="glnT"
                     /locus_tag="EYO68_06785"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HIC07546.1"
                     /translation="MSINLANVAREKGIRYFLISFTDLFGVVRSKLVPTSAISGMQKD
                     GAGFAGFAAHLDMTPADSDMFGLPDAERLIQLPWKPEVGWLPSDIYLGGKIVAHGPRT
                     VLKQVLADAKEKGYLVKTGVECEYFILTPEGTEVADSADIQAKPCYDQQALMRRYDLI
                     SEICDHMETLGWGPYQNDHEDANGQFEMNWDYTEALTTADRHSFFKYMTKTVAERHGF
                     RATFMPKPIATLTGNGCHAHVSVWNKTGKINLMEDKKGELGLSKLAYHFIGGLLEHAE
                     SLAAITNPVVNSYKRINAPRTTSGATWSPNTVTYSGNNRTHMIRIPDSDRIEVRLADG
                     AANPYLLQAVLIGAGLWGVANKIDPGKRLDIDMYAEGHKVKNAPKLPLNLLDALRSFQ
                     NNKTMRSILGAEFA"
BASE COUNT         1621 a         1477 c         1336 g         1642 t
ORIGIN      
        1 gttctgcaac tttgaagcgc atcacaaact gattttcctc tgggactcca tagactgcaa
       61 atcccgttga gtcgggacca cgatgcttca atgactgaag catcgaagtc atctcccccc
      121 ctatgtttcc gggactcccc cggcggataa ttcctgcaat tccgcacatt ctgctttctc
      181 ctgtctgaat ttgattattt tctggtttct agtttatagt taaggcaaac aatcaatgta
      241 tgtttccaaa tcccactccg ttacagtgtg gttaaatctt tcccactcgt catttttgta
      301 atgatggaaa acccgataca tttcatccgg catagacgat tttatgacct cgtcatccgc
      361 cagcttttgc aaggcttcac caagtgacat tggcaatttt ttgacttgtt taccttcttc
      421 aattgcttga taaatattgc gttcttctgc ttctccggga tccagatcgt tgctgatgcc
      481 gtcatcgaaa gctttgagca atgctgctcc catcaagtag gggttgacca tcgagtccac
      541 agaacggtac tcaaaacgac ccggcgcaga gatccgtaat gcacaagtgc ggttttgata
      601 accccaatct gcaaaaactg gagcccaaaa accagtatcc cacaatcggc gatacgaatt
      661 tactgtggaa gaaccgatgg aagtcaaggc tcccaaatgt ttcatcactc caccgataca
      721 ttgcaatccg attgggccgg gtttgcgttc atcaatggag ggatcgggca tgaaggtatt
      781 atctccacca acaagataag taaatgctcc tgccattccc ggaagtgttg cattgtgcag
      841 atcgttgacg gtatcttttc cgccacgcca gagtgacatg ttgtgatggc atccggaagc
      901 agaaacgccc ataaacggtt tggacataaa acaggcaatc aagccgtctt cacgcgccac
      961 ttgtgcgcaa atttggcggt aagtcgtcaa gcgatcagca ttacgcaaaa catcgtcaaa
     1021 catcgtgttc aactccaatt gtccgggagc gtcttcgtgg tcaccctgaa tcatgtccaa
     1081 acccatggcc tgtgagtatt caatgacttt catgaaagtt gggcgcaact cttcaaattg
     1141 gtcaatgtga taacaattcg gtttggtcac accgccattt ggccgtccgt cctcaccgcg
     1201 cttgagccac atcatttccg gctccgtacc gcatctcaag tgcagtccgt catatttatc
     1261 ctgaaactca ttgtgaatga tgcgcaaatt tccgcggcaa tctgaagtca aatgtcctcc
     1321 cggattttcg cgttcttcac ggtttcggaa acaagtacag aatactcttg caaccctctt
     1381 gtcccagggc agttgactga aggtttcagg atcaggaata ccgactaatt cggatgattc
     1441 aggcccataa ccaatgtaat caccgtggcg gtcaacatac aggttggcgg tagaaccgta
     1501 cacgagttgg aaccctctct cggcggtacg ctcccagtga tcggccggaa tacctttgcc
     1561 gacgatccga ccggtaacgg aaatgaattg atagtaaata taggtgactc ccagttcgtc
     1621 gattttttca cgaacctgct tcactaattt gtctcgcccc tcggcgttaa cgtgtgcttc
     1681 taagtctgtc aaattacctc ctgaaattag cagttgtatc cgtttttatt gctgaaaaat
     1741 gaacggatat tataaaggcc gaaaactaaa tcagttttct cagccatttt aattaaacct
     1801 tctcaacttt tgctcttctt ctattttttc aaacaatgaa tctacttcat ccaatactgg
     1861 aagtcaagag cgcggtttta gtttttccgg atcataaaac ggaaatttca ccacctgcgc
     1921 cggaatacgc ttttggtgtc cgtccagttt gccgatttga acttcagtgc caatttcggc
     1981 atgagttaca tcaatccggc aaagcgcaat gttcttgcgc aaaatcggtg agcgcattcc
     2041 gctggtgatt actccgactt gtccacgacc aatgtgaacg cagtctccgt gagcaccagg
     2101 ttcgttgcct tcaagctcca aaccaaccag ctttcgctga ggattttctt tgcggctaac
     2161 gagtgcttct ctgccgacaa aatcgtcttc ctttgttttc atgggtacag tgaaaccgac
     2221 tccggattca aacggatcgg tttcatcact gaactcgtag ccggcaaata caagtccgga
     2281 ttcaatgcgc accatatcta gggcatccaa acccaaaggc atcattccgt gaggttgtcc
     2341 ggcttcccat attacatccc aaaccgcagg tgcatcattg gggtggcacc agacttcgta
     2401 gcccagttct ccggtgtatc cggtgcggga aatcataatc ggaattccgt tgaggtctcc
     2461 aacgcgccca atcgtaaaac ggaaccagcc gatttcctcc agttttggtt gcgtgggcgg
     2521 agtccaaacg acttctttta gaatttcacg gctcttcggt ccttgaacgg caatgttgtg
     2581 aatttggtct gtcgaggatt tcacacgaac tttgaggcct aatttttctg cctgttcccg
     2641 catccaaatt ccgccgtaat cgtctccgcc aatccagcgg aaattatcct ggcacaagcg
     2701 caaaagcgtt ccgtcgtcca tcattccacc atgcggatat aacattgacg aataaacgac
     2761 ttgcccgaca gcgagtttac gaacattacg ggtcagagtc cattgcaaaa gtgcttccgc
     2821 atctggacca attacctcaa atttacgaag cggagaaagg tcggtgacaa tcgcgttttc
     2881 tcggcacgag tgatattctt caattgcacc gttatttctg taacaagtcg gtaaccaata
     2941 tccccgatat tctgtgaagt tgcgcgtcag ggccgaagtt cggggatgaa agccggtttc
     3001 ctgagttagt ttcgcgtcgg catctggagt cattctaatg gctattgctt tggagaaagt
     3061 gtttttttcc ggataaaccc ggacgtgaat atcggtagga ttccaaccgt tgacaggagt
     3121 ggtgtcgtcc ggacaggctg aagaaatgca aacaagatcc gtctgtgcct gcaacaaaac
     3181 ataatcaccc ggacgtgacc atgattcttc gccataaagc acattgtggt cgtcaattcc
     3241 ggtgttgaaa aagaaatttg ccgccgccca acctttgcgg ggttcgattt cgtattgggc
     3301 gagtgcgccg ttgaaattat cggaacagtt gatgtgtccg aaatagcccc ggtcctcata
     3361 cgattttgca gtacaggcca aaccgaatgt gtcatgccgt cccacagtgt cacgaatcac
     3421 ctcgaccaaa ggctgcatat caacatcgta atatttggaa aaaagaccag gccccggata
     3481 accggcccca atcagggtgc gggttgtcgt tacgtccaaa catcgctcga taccttgatc
     3541 taatttccgg tgatcgaaga cctgaaaatc agaacattca cggcccatca catcaattac
     3601 ttgaataaat tcaccggctt taacttcata ggcctgggaa gtgcagcggt caatttggaa
     3661 atcgagtcgg ggttctgcca acggatcagg caagtcggtt tcacgctctt ccggaggaga
     3721 tgctctatgg atgaaaactt ggagatcagt cggcggcaat gtttcccctt cgactgtcat
     3781 cattgtacca ggtgcggcta cggcacatgt agttcgggaa gttacctgaa agcgaagttc
     3841 ctctctcgct aaagaatctg gagcaaaaag aacagcggct tttgcggaag cgaggtctat
     3901 tcctttacgt tctaaaccag aacgtactcg accggcactt tcagaatcac cggaaagaat
     3961 ttggcgtaaa ccgtcagcgg gctgtgattt gttaattccg agaattccag gatcaccttt
     4021 gccttccggt gaaaatggga cgacttcagc atgctgacca ccttcaggat cctgtactac
     4081 gagaacatca tcgggatata actccacaac aatcacacca caagccggaa cgcggtaacg
     4141 ttcccgggag ggatccaatt gccagagtcc cggttccaaa atgccggaag tagttgttac
     4201 tttcgttaac tccatggttg atttatttga agttttaaaa caacaaaata gtattaattg
     4261 ttaactcgac agtcacgagt tttttcgtgt catcccgaac gtatgtgagg gatcttatta
     4321 agatatcagc actttatcag atctcttcct gcgatagaaa taacactccg caatgaatga
     4381 tgccagtatc ttacgagaat tcgtgaatca cgagatgttt aagtgttagc aacaatacga
     4441 ttaatccacg agtcttacaa taccctaaaa gataggatcc tcatttctta tgagtaggat
     4501 aaaactaccc ttacaggtgg tattaataat aaattttaat caattccagc ctgtaaattc
     4561 ttttctctca aattaatcgt taaatcaagt actctccatt gagaggtagt atcttgtaat
     4621 acattaatca ggtcaagtaa ataattcaaa caaataaaaa ccttgtcttt cctccttccg
     4681 ctctgttata tttccatgcg gtttatcgct aacagtttgc tttctgattt atttagagca
     4741 tcagaaataa ttaaggctta agacaaagta tttctattgt catcaaggtc tttatccggg
     4801 ataaagttgt tatttctgta aatgaaagtt aaaccaatta aaaatcaaaa aggacaatta
     4861 tgagtattaa tttagcaaat gttgccaggg aaaaaggcat ccggtatttt ttaatcagtt
     4921 ttacggactt gtttggagta gtgcgctcca agttggttcc cacctccgcc atttcaggta
     4981 tgcaaaaaga tggggccggt tttgcgggct ttgccgcaca cctcgatatg actcctgctg
     5041 actcggatat gtttggactt ccggatgcag agcgcctgat tcagttgccg tggaaaccgg
     5101 aagtcggctg gttgccgagt gatatttatc tgggcggtaa aattgttgcg cacggtccac
     5161 gaacagtact caagcaggtt ctggctgatg ccaaagagaa aggctatttg gtgaaaaccg
     5221 gagttgaatg tgaatacttc atcctcactc cggaaggaac ggaggttgcc gattcagccg
     5281 atattcaagc caaaccttgt tatgaccagc aagctttgat gcggcgttat gacctgattt
     5341 ccgaaatctg cgaccacatg gagactcttg gctgggggcc ttatcaaaat gaccatgagg
     5401 acgcaaacgg tcagtttgaa atgaattggg attatactga agccttgacc accgcggacc
     5461 gccacagctt tttcaaatac atgacaaaga cggttgccga aagacatgga ttccgcgcga
     5521 ccttcatgcc aaaaccaatc gccacgctga ccggaaacgg ctgtcacgca cacgtttcgg
     5581 tttggaacaa aaccggaaaa ataaatctca tggaagataa aaaaggtgaa cttggtctct
     5641 ctaaacttgc ttatcatttt atcggcggat tactcgaaca tgcagaaagt ctggccgcaa
     5701 tcacaaatcc tgtagtcaac agttacaaac gcatcaacgc tccacgcacc acttccggag
     5761 caacctggtc gccgaataca gttacttatt ccggaaataa ccgaacccac atgatccgta
     5821 ttcctgattc agatcggata gaagtccggc ttgcagacgg tgcggcgaat ccatacttgc
     5881 ttcaagcagt cttaatcggg gctggtttgt ggggtgttgc aaacaaaatc gatcctggaa
     5941 aacgcctgga tattgatatg tatgccgagg gacacaaagt taaaaatgca ccaaagcttc
     6001 ctcttaactt actggacgca ctgcgttcct tccagaataa caaaactatg cgcagcatcc
     6061 ttggcgctga gtttgc
//
LOCUS       DTVD01000198            6055 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4122_length_6055_cov_460.245333, whole genome
            shotgun sequence.
ACCESSION   DTVD01000198 DTVD01000000
VERSION     DTVD01000198.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4122_length_6055_cov_460.245333"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            181..1284
                     /locus_tag="EYO68_06790"
     CDS             181..1284
                     /locus_tag="EYO68_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S58 family protein"
                     /protein_id="HIC07547.1"
                     /translation="MQPGPKNSITDVSGIKVGHAQDMKLMSGTTVVIPDEPAVAAVDC
                     RGGAPGTRETDALHPANLVEEVHAVVLSGGSAMGLDAASGVAAWLKSAGRGFPVATDV
                     RVPIVPSAILFDLLNGGDKSKMDEHTYFEFGKSAVASADLECPLGNIGAGTGASAGTL
                     KGGIGTASLQQKSGILTGGPGSGNVGFTVGALAAANPFGSVTLPGRADFWAWPFERNG
                     EFGGRGAPKFEPESEQKKINELSLDFDFESPFQQTEKSSEKSAAGSVSMNTTLCVVAT
                     DAALTKAQSQRVAIMAQDGFARAIRPVHTPFDGDTIFVLATGKIPLSAMPPVDIARIG
                     MMAADCVARAISRGVYAAESLGIWTSYRDSIRI"
     gene            1385..2878
                     /locus_tag="EYO68_06795"
     CDS             1385..2878
                     /locus_tag="EYO68_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC07548.1"
                     /translation="MKSIFKKYAKLLLISGSALIFSGSAFAAKTHLTMGMSLEPPHLD
                     PTAGAAAAIDEVVYANVFEGLTRINQNAEVLPALADSWIISSDGLTYTFSLRRGVKFH
                     DGAAFDSSDVVFSLERATAENSTNAQKALFSAITAVKAQGANTVVVTLKTPTGNFLFN
                     MGWGDAVIVDSASAGSNKSKPIGTGPFTFKRWVKGDRIELGQNPNYWGKKPALKTATF
                     KIIPDPAAAVAALMAGDVDAFANFPAPEMAAQFQSDPSFTVKVGSTEGETILSTNNQQ
                     KPFNDLLVRQAIAHALNKQEIIDGAMFGFGTPIGTHFAPHHAAYVDLTGTYPHDPVKA
                     KQLLAEAGYPNGFKATLKLPPPSYARRGGEIIASQLRKVGINLEIIPVEWAQWLDQVF
                     KNKDFDLTIVSHTEPMDIGIYTRPKYYFQYRNSGFNAVIESLNVTSDPKRRYALMGAA
                     QAILAKDAVNGFLFQLAKLGIWNKNVNGLWENSPVQANDLTGVSWNN"
     gene            2933..3886
                     /locus_tag="EYO68_06800"
     CDS             2933..3886
                     /locus_tag="EYO68_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07549.1"
                     /translation="MTTFLAKRLFALLITLLVASLVIFLLLEILPGDPASIILGVGAR
                     EDTLAALRTEMGLDLSAPERYFRWIIGILQGDFGRSYTYDTPVSELILERLSLSLPLA
                     LLAISLSTLLAIPFGVFAAANHKRFADTGIMGFAQLGVAVPNFWFAILLILFFSVKLG
                     WFSAGGFAGWEMGLGAALKSLVLPAVALALPQAAILARVTRSAVLETVREDFVRTARA
                     KGLSRNAALWRHAVRNALIPVVTILGLQLSFLLAGTIIIENVFYLPGLGRLLFQAIAQ
                     RDLVVVKNIVILLAATVVTINFLVDMFYAALDPRLRSGGHE"
     gene            3879..4730
                     /locus_tag="EYO68_06805"
     CDS             3879..4730
                     /locus_tag="EYO68_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07550.1"
                     /translation="MSKSRIHEIWQQSWQHRSFVIGIILSFIMLLTGVVSLFWTPYSV
                     ELLDIPHKLQGPSTEHWLGTDHFGRDVFSMLMVGAWNSMLVSVFAIGFGAGIGVPLGA
                     LAAGKRGWVEETVMRFNDFAFAFPALLTAVMLSAVYGPGTINSVLAIGIFNIPVFARI
                     TRGSALSIYKREFILAARTSGKGEVRIAMDHILPNIASVLIVQGTIQFALGILAEAGL
                     SYLGLGTQPPMPSWGKMLSESQTMMFFAPQLAILPGLAIVLTVLGLNLLGDGLRDILD
                     PRLRRLR"
     gene            4827..>6055
                     /locus_tag="EYO68_06810"
     CDS             4827..>6055
                     /locus_tag="EYO68_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HIC07551.1"
                     /translation="MLKIIDVHVADIRFPTSSHSDGSDAMNPDPDYSATYVTLITNSA
                     FNGNGLTFTIGRGNELCVAAVHALKFMLINKDLVEITRNMGVFWRSLVGDSQLRWVGP
                     EKGVVHLATAAIVNALWDLYAKIEGKPLWKLLVDMSPEELVRAVDFRYITDVITPEEA
                     IKMLEENFSTRQQREAEMRLKGYPAYTTSAGWLGYSDDKIRDLCRDSIEKGWTHFKIK
                     VGSDIQDDIRRASIVREEIGSDCKLMFDANQIWDVEEAIENMKLLAQFDPWWIEEPTS
                     PDDVLGHQKISAQIAPIGVATGEHCQNRIIFKQFFQSKGLQFCQLDCCRLGGVNEVLS
                     VLLMAAKFNVPVCPHAGGVGLCEYVQHISIFDYICVSGSLENRIIEYVDHLHEHFENP
                     VIIKNGRYMPPLDPGFS"
BASE COUNT         1605 a         1288 c         1461 g         1701 t
ORIGIN      
        1 cagtcaatgc agttgatcta tttataaaat tatccaaaat gtctgaaact gcacacgtac
       61 aattaaaatc tcaacagaaa agcaggatca aaggacatga agtaatacgc ttcacactcg
      121 ctattgaatt agcaccacct acaatcaagc aaactcttaa tctcaacacc caaaaataat
      181 atgcagcctg gacctaaaaa cagcatcacg gacgtatctg gaatcaaagt tggacacgca
      241 caagacatga aattaatgag cggaactacg gttgttattc cggatgagcc tgctgtggcc
      301 gcagtagatt gccggggagg tgctcccgga actcgcgaaa cagatgccct gcatcctgca
      361 aacctggttg aagaggtaca tgcggttgtg ctcagtggag gctccgccat gggattggat
      421 gctgcaagcg gagttgcggc ctggttaaaa tcagccggcc gcggattccc tgttgcgacc
      481 gatgtcagag tacccatagt tccgtcagca attctctttg atttactcaa cggtggtgac
      541 aaatccaaaa tggatgagca cacttatttt gagtttggga aatcggcagt ggccagcgcg
      601 gaccttgaat gcccactggg caatatcggc gcgggaaccg gagcaagtgc aggaacactc
      661 aaaggtggaa tcggcactgc atccttgcag caaaaatccg gaatcctcac tggaggtccc
      721 ggaagcggaa acgtcggatt caccgtgggt gcactcgctg cagcaaatcc gtttggctca
      781 gtgacacttc cgggacgagc tgatttttgg gcctggccct ttgaacgtaa cggtgaattt
      841 ggagggcggg gtgcaccaaa attcgaaccg gaaagtgagc agaaaaaaat aaacgaactc
      901 tcacttgatt tcgatttcga aagccctttt cagcaaacag aaaagtcttc agaaaaatct
      961 gctgctggaa gtgtttctat gaacaccaca ctctgtgtag tagctacaga tgcagcactg
     1021 acaaaagccc agtctcaacg ggttgccatc atggcgcaag acggtttcgc ccgggcaatt
     1081 cgtcccgtac acactccttt tgacggagat acaatttttg ttttggcaac agggaaaatt
     1141 cctctttctg ccatgccgcc tgttgatatt gcgcgtattg gtatgatggc cgcagactgt
     1201 gtggcccgtg caatttcacg tggagtttat gctgcagaat cattgggtat ctggacctcg
     1261 tatcgggatt caattagaat ttaatttaat ttgctatttt ttagcttttt agtgatgatg
     1321 cctattcttc ttttttcaac acctccatga ggaggtttat taattcacat ctttggagaa
     1381 tataatgaaa tcaattttta aaaagtacgc aaaattatta ctgatcagtg gatctgcact
     1441 gatcttttcc ggatctgcat ttgccgcaaa aacacattta acaatgggga tgtcattgga
     1501 accgccacat cttgacccca cagcgggagc tgcagccgca attgacgaag tggtttatgc
     1561 caatgttttt gaaggtctga cacgcatcaa tcaaaatgca gaagtactgc cggcactggc
     1621 cgactcttgg attatttcct cagacggact tacctataca tttagtcttc gccggggagt
     1681 aaaattccat gacggtgctg catttgattc cagtgatgta gttttttcat tggagcgtgc
     1741 aactgctgaa aacagcacca atgcccaaaa ggcactattt tcagcaatca cagcggttaa
     1801 ggcacaaggt gccaatacag tggttgttac cctgaaaact ccaaccggca acttcctgtt
     1861 taacatgggt tggggcgatg cagtaattgt cgattcagca agtgcaggtt ccaacaaaag
     1921 caaacctatc ggtacaggtc cttttacttt caaacgctgg gttaaaggtg acaggattga
     1981 attggggcaa aacccaaatt actggggcaa aaaaccagca ctcaaaactg caaccttcaa
     2041 aattattcct gatcctgcag ccgctgttgc agcattgatg gcgggcgatg tcgatgcatt
     2101 cgctaacttt cctgcaccgg aaatggctgc acaatttcaa agcgatccga gtttcactgt
     2161 aaaagtcggc agtactgaag gtgaaacgat cctctctacc aacaaccagc aaaaaccatt
     2221 caatgatctc ctggtacgcc aggccattgc ccacgcactt aacaaacagg aaatcattga
     2281 cggagccatg tttggttttg gaactccaat aggaactcac tttgctccgc accatgctgc
     2341 atatgtcgat ctgactggaa cgtacccaca tgatccggta aaggccaaac aattgctggc
     2401 agaagccggt tatccaaatg gttttaaagc aacacttaaa ttaccccctc ccagttatgc
     2461 acgccgcggc ggagaaataa ttgcgtcgca gctgcgtaaa gttggaatta acctggaaat
     2521 aattccggtt gagtgggcac agtggctgga tcaagttttc aaaaacaaag actttgatct
     2581 gaccatcgtt tcacataccg agccgatgga cattggtatt tacacccgcc caaaatatta
     2641 ttttcaatac cgcaattcgg gcttcaatgc agtcattgaa agcctcaatg taacctctga
     2701 tccaaaacgc cgctatgccc tgatgggtgc agcacaggca attctggcta aagatgccgt
     2761 caacggcttc ctttttcagt tggcaaaact gggaatttgg aacaaaaacg tgaatggact
     2821 ttgggaaaac agccccgtcc aggccaatga cttgactggt gtcagctgga acaattaatt
     2881 tcctcaaagg cgcttcagga aattccctgg agcgccgggt ttaacaaatg aaatgacgac
     2941 cttcctggca aaacgattat ttgctttgct gatcaccttg ctggtggctt cactggtaat
     3001 ttttttgcta cttgaaatcc tgcccggtga tccggcttca ataattcttg gcgttggtgc
     3061 acgggaagac accctggccg ctctaaggac agaaatggga ctggatcttt ctgctccgga
     3121 acgttatttt cgctggatta ttggaattct ccagggtgat tttggccgaa gttacactta
     3181 tgatactccg gtttccgagt taattttaga acggctttcc ctcagtttgc ctttggccct
     3241 tctggcaatt tcactttcca ctttactggc cattcctttt ggagtttttg cagcagcaaa
     3301 tcataagcgt ttcgcagata ccggaataat gggctttgcg caactgggag tagccgttcc
     3361 aaatttctgg tttgcaatcc tgttgatctt atttttttca gtcaaactgg gctggttttc
     3421 tgcaggaggg tttgcaggct gggaaatggg tttgggagcg gcgctaaaat ctctggtttt
     3481 gcctgcagta gccttggcgt tgccacaagc cgcaatttta gcacgtgtta cccgttcggc
     3541 agttttggaa accgtaagag aggattttgt acgcaccgca cgtgcaaaag gtctcagcag
     3601 aaatgcggcg ctgtggaggc atgcagtcag aaatgcactg ataccggtag ttacgatcct
     3661 tgggttacaa ctttcatttt tgctggcggg tacaatcatc attgaaaacg ttttttattt
     3721 gcctggcctg gggcgtctgc tttttcaggc cattgcccaa agggatttgg tagtggtaaa
     3781 gaacatcgta atcttactgg ctgcaacagt agttacaata aattttctgg tggacatgtt
     3841 ttatgcagca cttgatccac gcttgcgtag tgggggtcat gagtaaatca cgaattcatg
     3901 aaatctggca gcaaagttgg cagcaccgta gttttgtgat tggcataatc ctgagcttta
     3961 ttatgctttt gaccggagta gtttctttgt tctggacacc ttattcagtg gaattactgg
     4021 acattccaca caagctgcag ggaccatcga ccgaacattg gctgggaacg gatcattttg
     4081 gccgggatgt tttttccatg ttgatggttg gagcctggaa ttccatgttg gtttctgtgt
     4141 ttgcaattgg tttcggagca ggaatcggag ttccgctggg cgctttggct gcaggtaaac
     4201 gtggctgggt ggaagaaact gtgatgcggt tcaacgactt tgccttcgcc tttcccgcct
     4261 tgttgacggc cgtcatgctg tctgcagttt atggtccggg aaccattaat tctgtactcg
     4321 caatcggaat tttcaatatt ccggtttttg ccagaattac ccgtggaagt gctttgagca
     4381 tctacaaacg tgaattcatt cttgccgcaa gaacttcagg aaaaggagaa gtccggattg
     4441 caatggatca tattttaccc aacatcgcga gtgtcctcat agtacagggt acaattcaat
     4501 ttgccttggg gattcttgcg gaagcaggtc tgagttacct gggacttggt acacaaccac
     4561 ctatgccaag ctggggtaaa atgcttagtg aatcacaaac aatgatgttc tttgcaccac
     4621 aacttgcaat actgcctgga ctggcaattg tcctcactgt tcttggcttg aatctgctgg
     4681 gagacgggtt acgggatatt ctcgaccccc gtttgagacg cctgcgctga atcagatttg
     4741 tttttaacat gattgcagga tttaggtaaa aaaaatatca acatgttaag ctttaaatat
     4801 tattaattat ttttgaggag atctcagtgc ttaaaatcat tgatgtacat gtagctgata
     4861 tacgtttccc tacatctagt cattcagatg gttccgatgc tatgaatccg gatccggatt
     4921 attcagcaac atatgtaact ttgattacaa attctgcatt taatgggaat gggctaactt
     4981 ttacaatcgg cagaggtaat gaactgtgtg tagctgcagt tcatgcctta aagtttatgc
     5041 ttataaacaa ggaccttgtt gaaattacac gcaacatggg agtattctgg cgttcgcttg
     5101 tcggtgattc tcagcttcga tgggtaggtc ctgaaaaagg cgtggttcac cttgctactg
     5161 cagcaattgt taatgcgctg tgggacttat atgcaaagat tgaaggaaag ccgctctgga
     5221 aattactagt tgatatgtcg ccggaagaac tggtgagggc cgtagatttt cgatatatta
     5281 cggatgtaat aacacctgaa gaagccatca aaatgcttga agaaaatttt tctacacgac
     5341 agcagcggga agctgaaatg cgtttaaagg gctatcccgc ctatacaact tctgcaggct
     5401 ggttgggcta ttcagatgat aagatcaggg acctgtgtag agattcaata gagaagggct
     5461 ggactcattt taaaataaaa gttgggagtg atattcagga tgacataagg cgggcctcaa
     5521 tagtaaggga ggaaattggt tctgattgta agctgatgtt tgatgcaaat caaatttggg
     5581 atgttgagga agccattgaa aatatgaaat tattggctca gtttgatcca tggtggatag
     5641 aggagccgac tagtccggat gatgttctgg ggcatcaaaa aatttcagct cagattgcac
     5701 caatcggtgt ggctacagga gaacattgtc aaaaccgtat aatttttaag caattttttc
     5761 agtcaaaggg actgcagttt tgccagcttg attgttgtcg tttgggtgga gtaaacgaag
     5821 ttttgtcagt cttgttgatg gcggcaaaat ttaatgtccc agtttgtccc catgcgggtg
     5881 gagtagggct ttgtgagtat gttcagcaca tctctatttt tgattatatt tgtgtgtctg
     5941 gcagtttgga aaatcggatt attgagtatg tggatcacct tcatgaacac tttgagaatc
     6001 ctgtgataat aaaaaacgga cggtatatgc ctcctctgga tccgggattc agtat
//
LOCUS       DTVD01000199            6047 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4134_length_6047_cov_240.943091, whole genome
            shotgun sequence.
ACCESSION   DTVD01000199 DTVD01000000
VERSION     DTVD01000199.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_6047_cov_240.943091"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="EYO68_06815"
     CDS             complement(<1..989)
                     /locus_tag="EYO68_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07552.1"
                     /translation="MEIVLENFTKRFGDLTVIENMNLKIKEGEMLALLGPSGCGKSTT
                     LFAICGIHRVDEGRVLFGGQDVTRVPTQQRNIGVVFQSYALYPHMTVAQNIAFPLTVR
                     HEDKQTIKKKVGEMAELVHIEELLGRRPEQLSGGQQQRVALARALIRKPGALLLDEPL
                     ANLDAKLRLEMRSEIRRVQLETGISAVLVTHDQVEAMSMSDRIAIMKDGKILQVASPN
                     EMYQRPENDFIAGFLGNPPIAFLDGIISAGNVKISSGNIEFPCPENIEKHPDGSHISV
                     GIRPEFYQPGNPYKIPGTVSFVEIQGRENLYDIKLKDGNLLRSIQSANVSPLSP"
     gene            complement(989..1813)
                     /locus_tag="EYO68_06820"
     CDS             complement(989..1813)
                     /locus_tag="EYO68_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC07553.1"
                     /translation="MSHNFKNWPIVTGLTLVTIPILFMYVFLFVDTVTDTRPGEMIPN
                     RFTLEHWRFLWENIEGKASVWLAMLNTMIFASSVTLIVLSVSSTASYAISRLNIPFRK
                     EFMAGILMLHAFPSITLIIAIFLILQMVGLYNTLIGVILVKASLQLPLGIWIMKGFYD
                     SVPWEIEMAGIQDGASRFTVWYRLVLPQVRPGFFALGLFSFLEGWSEYILPAVLAPAN
                     DVQMLSVYLASLIADDQHFDFNLFKSVGLFYAIPVIIFYLFFQDKLMNIYGGGTKG"
     gene            complement(1823..3127)
                     /locus_tag="EYO68_06825"
     CDS             complement(1823..3127)
                     /locus_tag="EYO68_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC07554.1"
                     /translation="MSAKLGYIFIAPALALVVLFFLTPVLMTGVFSMTNMSTATGITG
                     GSYQITPSVLSQLGDQGFDRKILKRIGSESYSISEKGLLAATKADVKTELVEELRERY
                     LGETFASRRLFERFLKKLDNRPRSIRTLKSTSPHFRKSLIKERFASTKELLQSLEEMD
                     LGLTQVQTDELVDASYTGWIWTGENFRKMFLLPETRQILLNTGFYVASTLLLFNIGFA
                     LVLAVTTFYLPKGQAALFRALWFLPRITPSVLYVLLWKWLTWDTGFINAVVTNFGVTP
                     RNWMLDTAANAWVTVIMINGFIGASMGMILFSSAITSIPKQMLYASEVDGANRWQQIR
                     HIILPQLKWPILFITVYQTLSLLTSFEQIFLSTDGGPGSSTEVWALSAYHTALDNYWG
                     NLQYGYGAALALVLVVVGVIMSLIYLRFFRFDELIKTPRIEQ"
     gene            complement(3202..4575)
                     /locus_tag="EYO68_06830"
     CDS             complement(3202..4575)
                     /locus_tag="EYO68_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIC07555.1"
                     /translation="MLAFKSFSKRFALGIVGVGVSFGLSLTPVMAKDITIQVWAGGSN
                     VNDSYRVDAITMAADILEKEAAILGEDLNITVETKYDFDGWGGFKQAVTLAAEANKAP
                     HIVVTGHEDIAPWSQSGLIRPIENYVDFDTWPLSDLFENLVEIASFEGTIYGVPQDAE
                     SRPFFAWIPHLKAIGYSKADIRALPGKILSGNYTLYDVLDDAKKIQDAGLVKPGYGFY
                     PRVSKGGDYWQFYLSFGGEMSDSKSGKLVFDRSAMKNFYTFFAKAVEMGVTRKNHIGT
                     PWDQWYSEVASGKAGLWHGGTWHYSRYTGKEGLKDFFGNIVFGLIPAGDKNGRANTIT
                     HPLVYLVTNRGSEDDAQIAAQLVAIASEPRINTLHAIKSAHLGITKSQQNVPLYANNR
                     WASEATKLLLPHASAMPNHTNFSPYFDAMWKGLEASWTGQKSPEKAVADVEAELRANL
                     GNDIIIR"
     gene            4849..5664
                     /locus_tag="EYO68_06835"
     CDS             4849..5664
                     /locus_tag="EYO68_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIC07556.1"
                     /translation="MDYDHLRNELVGNYSNLSRRLKQITRYAMSNPNDMALETIVVIA
                     ERADVPPSSLIRFAKSFGFNGFREMQKVFQQGLVKSSSDYRKRVQTLNRQLSQQGEVA
                     LSNLENFINGGIEALHGLRESVTDEQIENAASILAKANVVHVAAQHRSFPVAAYLTYA
                     LSHLSVPNILLDSVGGMFAERGKTVRKGDVVLAVSYSPYANEVLKLVEVVQERKVPVI
                     VITDSVLGPLAPFADVCLEVKETEVFGFRGLVLSALMCLSLSLIVELGKQLEH"
     gene            complement(5661..>6047)
                     /locus_tag="EYO68_06840"
     CDS             complement(5661..>6047)
                     /locus_tag="EYO68_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIC07557.1"
                     /translation="AGDIDTAVISLKFANGALGIIENSRKAVYGYDQRVEVFGAKGTA
                     MADNNTPTSMVVLNESGTIRDKPLYFFLERYKIAFVAEMQAFVDAVRNDEPTKVSGKD
                     GLVPVLIALAAQESLKTGKPVQVVSV"
BASE COUNT         1750 a         1394 c         1338 g         1565 t
ORIGIN      
        1 ggagagagag gagaaacatt agcggattgg atcgaacgca ggaggttacc atctttcaat
       61 ttaatatcgt aaagattttc acgcccctga atttccacaa atgaaactgt tcccggaatt
      121 ttgtagggat tccccggctg gtagaattcc ggtctgatgc caacgctaat atgggaacca
      181 tccggatgtt tttcaatatt ttctggacat ggaaattcaa tattcccact gctgattttt
      241 acatttccag cagaaatgat tccatccaga aacgcaatcg ggggattgcc taaaaacccg
      301 gcgataaaat cattttccgg acgttgatac atctcattcg gagatgccac ttgtaaaatt
      361 tttccgtcct tcataatggc aatacggtcg ctcatgctca tcgcctccac ttgatcatgt
      421 gtcactaaca cagccgaaat accggtttcc aattgaacac ggcgtatttc agaacgcatt
      481 tccaaccgta gttttgcatc aaggttggcc agcggttcat ccagcaacag cgctccgggc
      541 ttgcggataa gagcacgggc cagggccacc cgctgctgtt gtcctccgga aagctgttcc
      601 gggcggcgcc ccagcagttc ttcaatgtga accaactccg ccatttctcc cacttttttc
      661 ttgattgtct gtttatcttc atggcgaacg gtcaaaggaa aagcgatatt ctgagcaacc
      721 gtcatgtgag gataaagagc ataagactgg aaaacgacac caatattgcg ttgttgagtt
      781 ggaacccgcg tgacatcctg gccgccaaaa agaacacgcc cctcgtcaac tctatgaatc
      841 ccgcaaatgg caaaaagcgt ggttgatttt ccacagccgg acgggcctag caaggccagc
      901 atttcgcctt ctttgatctt caagttcata ttttcgatga ccgtcagatc accgaaacgc
      961 ttggtaaaat tttccaggac aatttccatc agccttttgt tcctccgccg taaatgttca
     1021 ttaacttgtc ctgaaagaac agataaaata tgatcaccgg aatggcgtaa aacaagccca
     1081 cagatttgaa cagattgaag tcgaaatgtt ggtcatccgc aatcaaggaa gcgaggtaaa
     1141 ccgacaacat ctgcacatca tttgcggggg ctaaaacagc cggtaaaatg tattcactcc
     1201 agccttccag aaatgaaaat agtccgagtg caaaaaatcc cggtcgaact tgtggcagaa
     1261 ccaagcgata ccaaaccgtg aaccggctcg caccgtcctg gattccggcc atttcgattt
     1321 cccatggaac cgagtcataa aatcctttca tgatccaaat tcccaaaggc agttgcaaag
     1381 aggctttgac caaaatgact ccaatgagcg tgttgtaaag gccaaccatc tgcaaaatca
     1441 ggaaaatcgc aataatcagt gtaatcgagg ggaatgcatg caacatcaat attcctgcca
     1501 taaactcttt tcgaaaaggg atattgagac gagaaattgc atacgatgca gttgaggaaa
     1561 cggataagac gatcaatgta accgaacttg caaaaatcat tgtattaagc atagccagcc
     1621 aaacactcgc tttgccttca atattttccc acaaaaaacg ccagtgttcc aaagtgaagc
     1681 ggttaggaat catttcaccc ggacgcgtgt ctgtgaccgt atccacaaac agaaaaacgt
     1741 acatgaacaa gattggaatc gtgacgagtg tcagtccggt aactatgggc caatttttaa
     1801 aattatgcga cattgcggat tcctattgct caattcttgg agttttgatg agttcatcaa
     1861 aacgaaaaaa ccgaagataa attagcgaca taatgactcc aaccacaact aagaccaaag
     1921 caagagcggc tccatagcca tactgcaaat ttccccaata attatccaaa gcagtgtgat
     1981 aagcggacag tgcccaaact tcagttgaag agccgggacc tccgtcagtg gaaagaaaaa
     2041 tttgttcaaa agaggtcagc aatgacaagg tttgataaac ggtaataaaa agaatcggcc
     2101 atttgagctg gggtaaaatg atatgacgaa tttgctgcca acggtttgcg ccatcgactt
     2161 cgctggcata aagcatctgt tttggaattg aggtaatggc actggaaaac aaaatcatgc
     2221 ccatcgaagc accaatgaaa ccattgatca tgatcacagt cacccaggca ttggccgcag
     2281 tatccagcat ccagttacgg ggcgtgactc caaaattggt cactaccgca ttgataaatc
     2341 cggtatccca agtcagccat ttccacaaca aaacatacag aaccgaaggc gtaatcctgg
     2401 gtaaaaacca gagcgccctg aaaagagcgg cttgtccctt tggcaaataa aatgtggtaa
     2461 cggccaaaac tagggcaaaa ccgatattaa aaagcaagag cgttgaggcg acataaaatc
     2521 cggtattgag caggatttgc ctggtttccg gaagcaaaaa catcttgcga aaattttctc
     2581 cggtccaaat ccaacccgta taacttgcgt ccaccaattc atcggtttgt acctgtgtca
     2641 aacccaaatc catttcttcc agtgattgaa gcaattcctt tgtactggca aatcgttctt
     2701 tgatcaatga cttccggaag tgcggtgagg tggatttgag agtacggatg cttcgaggtc
     2761 ggttgtccaa tttttttaaa aagcgttcaa aaaggcgacg actggcaaag gtttctccaa
     2821 gataccgttc ccgtaattcc tcaacgagtt ctgttttcac atcggctttt gtcgccgcca
     2881 ggagaccttt ttcactgatg gaataacttt cagaaccaat gcgtttcaag attttgcgat
     2941 caaaaccctg atctcccagt tggcttaaaa cggagggcgt tatttgatac gagccaccgg
     3001 taattccggt ggccgtactc atgttggtca tagaaaatac gcccgtcatg aggacgggcg
     3061 ttagaaagaa gagaacaacg agggcaagtg caggagcaat aaagatgtat ccgagtttgg
     3121 ctgacacgta atttcctgat gtcctgtgat tcgttgttcg gtcagcaacc gtcgggcaat
     3181 gcccgccggt tgaactgatt tttagcgaat gataatgtcg ttgcccaaat tggctcgcaa
     3241 ttcggcttcc acatcagcaa ccgctttttc cggagatttc tgccccgtcc aagaagcttc
     3301 cagacctttc cacatggcat caaaataagg agagaaattg gtgtggttag gcatcgcact
     3361 ggcatgtggc aacaaaagtt tagtcgcttc gcttgcccaa cggttgttgg cgtagagcgg
     3421 aacattctgc tgagacttgg taatgcccaa gtgagcggac ttaatcgcgt gcaaggtgtt
     3481 gatacgaggc tccgaagcaa tggccacgag ttgggccgca atctgagcat catcttcact
     3541 tccacgattt gtcaccaaat aaaccaacgg atgggtgatg gtattcgctc gtccgttttt
     3601 gtctccggca ggaatcaagc cgaacactat gttgccaaag aaatccttca aaccttcttt
     3661 tccggtatag cgggaatagt gccaagttcc gccatgccaa agccctgctt ttccggaagc
     3721 cacctcagaa taccattgat cccagggagt tccaatatgg tttttgcggg tgactcccat
     3781 ttcaaccgct ttagcgaaaa acgtatagaa gtttttcatg gcactgcggt caaaaaccag
     3841 tttgcctgat tttgaatccg acatttctcc accaaaggaa aggtagaact gccagtaatc
     3901 tccgccttta gagacgcggg gataaaaacc gtatcctggt ttgacaagac cggcatcctg
     3961 aattttcttg gcatcatcca gcacatcgta gagcgtataa ttaccgctta ggattttgcc
     4021 gggcagtgcg cggatgtcgg ctttgctgta tcctatagct ttcaagtgag gaatccaggc
     4081 aaagaatggg cgagattccg catcttgcgg cacaccgtaa atcgtcccct caaaagacgc
     4141 aatttcaacc aggttttcaa acagatcact caacggccat gtgtcgaaat cgacataatt
     4201 ttcaatgggc cgaatcaatc cggactgtga ccatggggca atatcttcat gtccggtcac
     4261 cacaatgtgc ggggctttgt ttgcttctgc cgccaaagtc accgcttgtt tgaagcctcc
     4321 ccagccgtcg aaatcatact ttgtttcaac agtaatattg agatcctcac cgagaattgc
     4381 ggcttctttt tccaaaatat cggcagccat cgtaattgca tcaacccgat atgaatcgtt
     4441 aacgttcgag ccaccggccc aaacctgaat cgtgatgtcc ttcgccatca cgggtgtcaa
     4501 ggacaacccg aaggacaccc caacaccgac tattcccaaa gcgaaacgct ttgaaaacga
     4561 tttaaatgct aacataataa tctccttttc attagaaata ggatttttag cggccttaga
     4621 accattctaa ggtcaaatta aatgaaaaat atatttcatt tagttctaag catggaatat
     4681 acttcatata aaaaatttgt aaagctttaa tttaaatttc tgaaaataaa ttattttcag
     4741 attcaagttc aacccaattg ggtttcaagc gatgaaaata atcttccatt tttcctagca
     4801 tgaacttggc aatagctcaa cgaatcaccc aacactaaaa taataacaat ggattacgat
     4861 cacctgagaa atgaactcgt tggaaactac agtaacttga gtcgccgtct aaagcaaatt
     4921 acacgttacg ctatgagtaa tccgaacgac atggctctgg aaacgattgt agtaatcgca
     4981 gaaagggcag atgtacctcc gtcaagtttg attcgctttg cgaaaagttt tggttttaac
     5041 ggtttcaggg aaatgcagaa agtgtttcag cagggactgg tgaaaagttc cagcgactac
     5101 cgaaaacgtg tacaaacttt gaatcgacag ctcagccaac agggtgaagt tgcattgtca
     5161 aacctggaaa attttattaa cggaggaatt gaagctttgc atggattacg tgaatcagta
     5221 acagacgagc aaattgagaa tgcagcttct attcttgcaa aagctaatgt agtacatgtt
     5281 gcggctcagc accgttcttt ccctgtagct gcatatttaa cttatgcttt gagtcacctg
     5341 agtgtcccaa acatactttt agacagcgtg ggtggtatgt ttgcagaacg aggcaaaaca
     5401 gtccggaaag gagatgttgt actggccgtg agttattctc cttacgccaa cgaagtcctc
     5461 aaactggtag aagtggtgca ggagcgcaaa gttccagtga tcgtgataac tgacagtgtt
     5521 ctcggtcctt tagctccttt tgcggatgtt tgtctggaag taaaggagac agaggtattt
     5581 ggttttcgag gtttggtttt gagcgcattg atgtgtttgt cactttcgct gattgtggag
     5641 ttgggaaagc agttagaaca ctaaacagaa accacttgaa caggtttacc tgttttgaga
     5701 gattcctgtg cggccaacgc aattaagacc ggaacaagtc cgtcctttcc ggaaacttta
     5761 gtcggttcgt cattacgaac cgcatctaca aaggcctgca tttctgccac aaaagcgatt
     5821 ttgtagcgtt ccagaaaaaa gtagagcggt ttgtccctga tagttccgga ttcgttgaga
     5881 acgaccattg aagttggagt attattatcc gccattgcag ttccttttgc accgaacact
     5941 tcaacacgct gatcataacc gtagactgct tttcggctgt tttcgataat gccgagcgca
     6001 ccgttggcga atttcagtga aatgacagcc gtgtcaatat cgcccgc
//
LOCUS       DTVD01000200            6039 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4145_length_6039_cov_407.425468, whole genome
            shotgun sequence.
ACCESSION   DTVD01000200 DTVD01000000
VERSION     DTVD01000200.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_6039_cov_407.425468"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="EYO68_06845"
     CDS             complement(<1..1293)
                     /locus_tag="EYO68_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIC07558.1"
                     /translation="MSDKNSTPAPATDFIRNIISGDLDSGKHQHIVTRFPPEPNGHLH
                     IGHAKSICLNFGIANEFDGKCFMRFDDTNPVKEDEEYVEGILRDVRWLGFDWGERLTH
                     ASDYFEQLYLFAEELIKKGKAYVESQNADEIRELRGTLTEPGKNSPFRERSVENNLTL
                     FRKMRGGEFEDGTHVLRAKINMASPNINLRDPVLYRIRKISHQRTADQWCIYPLYDFT
                     HGLSDALEGVTHSLCTLEFEDHRPLYEWILAEVSAPCLPRQIEFSRLNLRYTVLSKRR
                     LIQLVEEGHVNGWDDPRMLTLSGLRRRGYPPSAIRLFCDRIGISKSENNIEMSVLEDS
                     AREELDKTAPRAMAVLRPLKVVITNYPEDKTEEFEPSRHPKNPEMGTRKVPFTREIYI
                     DHDDFRIDPPAKYFRLAPGKEVRLRFAYVIRCEEVIYDS"
     gene            complement(1479..2162)
                     /locus_tag="EYO68_06850"
     CDS             complement(1479..2162)
                     /locus_tag="EYO68_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIC07559.1"
                     /translation="MKKLYELAGADENRRFSPYCWRARMALAHKGLEVESLPWHFTEK
                     EAISFSGQERVPVLIDAENTISDSWEIAKYLETEYPDTPSLKLDHGEVLFIKFWVEIV
                     LHPELLQLLVMDIYNNLAQKDQNYFRESREKLFGKALEEIVINRNERLPRFQKLLNPL
                     RTTLKKQDFVAGETPGFSDYIVFGAFQWARCISEFSLLNADDSVYSWREKMLNLNDGL
                     ARNAVGYAV"
     gene            complement(2237..3436)
                     /locus_tag="EYO68_06855"
     CDS             complement(2237..3436)
                     /locus_tag="EYO68_06855"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIC07560.1"
                     /translation="MTSKKQNYLQQFELKYGCNPHQKPAAIHSLEGRKLPFSVLNGQP
                     GYINLLDALNAWQLVQELDEVLGLPAAASFKHVSPAGAAVSVPLNDILKEVYDCKDLE
                     LSQLATAYIRARGADPLSSFGDFISLSRKVDVNTAELIRKMVSDGIIAPGFDDDALKL
                     LKEKKGGKYLILLADTEFRAPETEFREVYGVGFSQLRNTVKINEKSLLTEVVTKKKQL
                     SAAARRDLILASITLKYTQSNSVAYALDGQMIGIGAGQQSRVDCTKLAGSKAETWFMR
                     QHPKVRNLPFHEQVSNVERTNARILCIDGGMTDPELRAWKSQFSAPVEALPQEEKAAW
                     LAKLKGVSLSSDAFFPFRDNIDQASKRGVNFIVQPGGSNRDEEVISASDEYEMVMAFS
                     GIRLFHH"
     gene            complement(3636..4157)
                     /locus_tag="EYO68_06860"
     CDS             complement(3636..4157)
                     /locus_tag="EYO68_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07561.1"
                     /translation="MIGACTDEVQIHKSALRYYSEGNEALKHRDYQVAIWNYQKAISL
                     DSESPNFHYNLGLTYYEIGNYSESIDSFKRVESLVPNQTDTYYNLALAHHKMSNSRLA
                     DIYFNRYQDMLSLRKAQERLEAVRKEQEREAKIAEADSKRKELKKSSPSTSKKTSLKK
                     KSKPGKSEVPNWE"
     gene            4409..4843
                     /locus_tag="EYO68_06865"
     CDS             4409..4843
                     /locus_tag="EYO68_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07562.1"
                     /translation="MQRNLFLLLSLCGFLLWKTPLLPVQPVFAQELDEQIEKEVKAEK
                     ERQKFIEEMQTLLDAAMKSGFSEKEIREITVTRNGKVVHIWDFLEQEKLREKKDALAR
                     KSSIPRDRYLTVMDIADELEGGETRDLDALKDKSIFVGAEEK"
     gene            4840..5433
                     /locus_tag="EYO68_06870"
     CDS             4840..5433
                     /locus_tag="EYO68_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIC07563.1"
                     /translation="MKILAVSGGSGCGKTLFTKLLVKRLPKSAVLPLDSYYKDRPVNI
                     PSEEYDFDIPQAFDFDLYHEHLDRLISGKSIKMPHFGYESGKRKTSFSEIDPSEYLII
                     EGLHVLLHSRVREMLSYSFYMESPLDVAICRMCLRDIKNFDVTAEYRLNQYLKFVRPA
                     YFKYIMPTKQYADIVIKNNYDSHLDEFVGDFLLNMKL"
     gene            5513..5938
                     /locus_tag="EYO68_06875"
     CDS             5513..5938
                     /locus_tag="EYO68_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07564.1"
                     /translation="MELLKRYSISFGIAGVLLYFFTIWVEKQHESNMPHNLTAKIEEI
                     VTIILPVKRYFEKCLDLSESQELQYGFDSQAPLSFNLHYHQKGEINYTFKEDSIDELE
                     NTFQPEKRGYYCLQWGNSGKEKVEMNYHFNILKRASVND"
BASE COUNT         1706 a         1194 c         1258 g         1881 t
ORIGIN      
        1 actgtcgtaa ataacttctt cgcagcgaat gacgtatgca aaacgcagcc gcacttcttt
       61 tccaggagcg agccggaaat atttggctgg tgggtctata cgaaaatcgt catggtcaat
      121 gtaaatttcc cttgtaaaag ggactttacg tgttcccatt tccgggtttt taggatgccg
      181 tgacggttca aattcttccg ttttatcttc cggataattt gtaatgacaa ctttcaaggg
      241 gcgtaaaaca gccattgcac gtggggcagt tttgtccagt tcttcacgtg cactgtcttc
      301 aagaacgctc atctcaatgt tgttttcgct tttcgaaata ccgatgcggt cacaaaacag
      361 gcggattgcc gaaggcggat aaccacggcg gcgtaatccg gaaagagtta acatgcgggg
      421 atcatcccaa ccgttaacat gcccctcctc tactagttga attaaccgac gtttgctcaa
      481 gactgtataa cgtagattga gtcgtgaaaa ttcaatttgc ctgggaaggc acggcgcact
      541 cacctcagcc aaaatccatt cgtacaaagg ccgatggtcc tcaaattcaa gtgtgcaaag
      601 cgaatgggtt acaccttcca gtgcatccga caagccatgt gtaaaatcat aaagcggata
      661 aatacaccat tgatcagctg tacgctgatg tgagattttg cggattctgt aaaggacagg
      721 atcacggaga tttatattcg gtgaagccat gttaatttta gctcgtaaaa catgagtacc
      781 gtcttcaaac tcaccgcctc gcattttcct gaagagtgtt aaattgtttt caacgcttct
      841 ctccctgaaa ggactgttct ttcctggttc cgtcagtgtt ccacggagtt ctcggatttc
      901 atctgcattc tggctttcaa cgtaggcttt cccctttttg atcagttctt cggcaaataa
      961 ataaagctgt tcaaaataat ctgatgcatg agtaagcctc tcaccccagt caaatcccaa
     1021 ccaacgcacg tcccgtaaaa tcccttcgac atattcctca tcctctttga ccggattggt
     1081 gtcatcaaag cgcatgaagc atttaccgtc aaactcgttt gcaataccaa aattcaggca
     1141 tattgatttt gcatggccaa tgtgtaagtg cccatttggt tcaggtggaa aacgggtaac
     1201 aatgtgctga tgtttccctg aatccaagtc tccactgatg atgttgcgga taaaatctgt
     1261 tgcaggtgcg ggagtagaat ttttgtctga catgtttggt tataaaatta tttgatgttt
     1321 tgatagacaa actcgtagga agttgtcatt ctcgcaaaag cggaaaccag gaacttgaac
     1381 gtatcttaaa taatagatta tctctgtcgc tggattgact gacaagtgtg atattttgat
     1441 tatttatgtc tgcatttgat tgattagccg tatctttctt agactgcgta tcccactgca
     1501 ttcctggcca gtccatcatt aaggttaagc atcttttcac gccaggagta aacagagtca
     1561 tcggcattaa gtagagaaaa ttctgaaata caacgtgccc actgaaatgc tccaaaaaca
     1621 atataatcag aaaagccagg agtttctcct gcaacaaaat cctgtttttt gagtgtcgtc
     1681 cttaatggat tcagtaattt ttgaaatcgc ggcaaacgct cattgcggtt tattacaatt
     1741 tcttccagtg ctttgccaaa gagtttttca cggctttccc gaaaataatt ttgatctttc
     1801 tgagctagat tattgtaaat gtccattacc agtaactgca gtaattccgg atgtaaaact
     1861 atctcaaccc aaaattttat gaaaagcact tcaccatgat cgagtttcaa tgaaggcgta
     1921 tccggatatt ccgtttccag gtattttgca atttcccatg aatctgaaat agtattttca
     1981 gcatcaatca aaaccggtac tctctcctgt ccagaaaaac taattgcttc tttttcagta
     2041 aaatgccagg gcaggctctc aacttcgagt cccttgtgtg ccagagccat gcgtgcccgc
     2101 cagcaataag gactgaatct tctattttca tctgcacctg ccaattcata taattttttc
     2161 ataaagtttc ctggttgatt cctgtaataa aataaaaaat tttaacttaa ttggagtcaa
     2221 gtgctgctaa aaataatcag tgatgaaaaa gtcgtattcc ggaaaaggcc atcaccattt
     2281 catattcatc cgatgcagaa atcacttctt catctcggtt gctgcctccg ggctgaacta
     2341 taaagtttac accacgtttt gaggcctgat cgatattgtc acgaaaagga aaaaatgcgt
     2401 ctgatgataa acttactcct ttaagcttgg caagccatgc tgctttttct tcttgaggaa
     2461 gagcttctac cggagcacta aactgacttt tccaggcccg aagctcgggg tcagtcattc
     2521 ctccatctat gcagagaatg cgggcatttg ttcgttcaac attactgact tgttcatgaa
     2581 agggcagatt ccggacttta ggatgctgtc gcataaacca tgtttcggct ttacttccgg
     2641 ccagtttggt gcagtccacc cggctttgct gacctgctcc aatgccgatc atttgtccat
     2701 ccaatgcata agccacagaa tttgactgag tatattttag tgtaattgag gccaaaatta
     2761 aatcgcgacg tgccgcagct gacagttgct tttttttcgt tacaacttca gttaataaag
     2821 atttttcgtt gattttaacc gtgttccgca attgcgaaaa accaacacca taaacttctc
     2881 tgaattcagt ttctggagct ctaaactctg tgtctgctag caaaattaag tacttcccgc
     2941 cctttttttc tttaagcagt tttagtgcat cgtcatcaaa tccaggtgca atgataccgt
     3001 ctgacaccat tttcctgata agctcagcag tgttaacatc gacttttcgg ctgagcgaga
     3061 tgaaatcacc aaaagaagat aaaggatctg ctccacgtgc cctgatatat gcagttgcca
     3121 gctgtgaaag ttccagatct ttgcagtcgt aaacttcctt taaaatgtca ttcagaggca
     3181 cactgacggc ggctcccgcc ggacttacgt gtttgaaaga agctgcagcc ggaagcccaa
     3241 gaacttcatc aagttcctgt accaactgcc atgcgttgag tgcgtccagc aaattgatgt
     3301 agcccggttg accgttcagt acagaaaatg gcagtttgcg tccttcaagt gagtgaattg
     3361 cggcaggctt ttgatgggga ttgcaaccgt atttgagctc aaattgctgc aaatagtttt
     3421 gtttttttga agtcattaaa ttctttgctc tttaagttga aggcgggatg tgtatgctgt
     3481 cacttagatg gtagctcttc aaactcaaag attcaaggat tcattggttc agcaggcggg
     3541 taagtaaata aagctgaatt agcagcagag ggtcagcaat aatccaattt agctgggtat
     3601 attaagtcag gactgacggt ctcatatcca gttggttatt cccaatttgg aacttctgat
     3661 ttgcctggtt ttgatttttt tttcaaggag gtcttttttg aagtggaagg agagcttttt
     3721 ttcagttcct tccgtttaga atctgcttca gcaattttgg cttctctttc ttgttctttc
     3781 ctaaccgctt ccagtcgttc ctgtgctttt ctcaagctca acatatcctg gtagcgattg
     3841 aaatagatat ctgccaggcg actgttagac attttgtggt gagcaagtgc caaattgtaa
     3901 tatgtgtctg tttgatttgg aacaagtgac tctacacgtt tgaaggaatc aatggactca
     3961 ctgtagttgc cgatttcata gtaggttagt ccaagattat aatgaaaatt aggagactca
     4021 ctgtccaaac tgattgcctt ctggtagttc caaattgcta cctggtaatc ccgatgtttc
     4081 agggcttcgt tgccctcact ataatagcgt aatgctgatt tatggatttg aacttcatct
     4141 gtacaggctc caatcataag cggaaacagg agcagcagga aaaatcgaga ttgaagattt
     4201 atagatttaa gatacactgg aaattcaata taaaacaggg tgtactctaa ataattctaa
     4261 aaagcaaaat tatgttagtt ttgtttgact ggtgatctct gcaaccgttc tcatttttgc
     4321 tcaaggattg cattattgat caggaatgtt tcccgtttcc aatttgtcgg aaatcaatca
     4381 tgcataattc atactcaaca acatactgat gcaacgaaat ttgtttctgc ttttatcgct
     4441 ttgtggcttc ctcttatgga agactcctct tctgccagta cagccagtct ttgctcaaga
     4501 actggatgaa caaattgaaa aagaagtcaa agcagaaaag gagaggcaga aatttattga
     4561 agaaatgcaa accttgctgg atgcagcaat gaaatctggt ttctctgaaa aggaaatcag
     4621 ggagattacc gtcacacgca atggaaaagt ggtgcatatc tgggattttt tggaacagga
     4681 aaaattgcgt gagaaaaagg atgcacttgc cagaaagagt tctattccac gtgaccgtta
     4741 cttaacagtg atggatattg cagatgaatt agaaggtggg gaaaccagag atttagatgc
     4801 cttgaaagat aaaagcattt ttgtaggagc agaagaaaaa tgaagattct tgcagtgtct
     4861 ggcggttccg gctgcgggaa aacgcttttt acgaaattgt tggttaagcg tttacccaaa
     4921 tcggcggtgt tgccgttgga cagttactac aaagaccgac ctgttaatat tccatctgaa
     4981 gaatatgatt ttgacattcc tcaagctttc gattttgatt tgtatcatga gcatctggat
     5041 cgtttgattt caggcaaatc tataaagatg ccgcattttg gttatgaatc aggaaaacga
     5101 aagacgagct tctctgagat agatccaagt gagtatttga ttattgaagg tctgcatgtt
     5161 ttgctgcatt ccagagtccg ggaaatgttg tcttattcct tctacatgga gtcgcctttg
     5221 gatgtggcaa tttgcaggat gtgtctgaga gatatcaaaa atttcgatgt aactgcagag
     5281 taccgcctta atcaatattt gaagtttgta aggcctgcct attttaaata tattatgccg
     5341 acaaaacagt atgcagatat cgttataaaa aataattacg atagtcatct tgatgaattt
     5401 gttggtgatt ttttacttaa tatgaaacta taatttttta tattgcttaa gtttgtgaac
     5461 gagaattgtt ttcttactgt ctcccccttc cacactattt aaaataacct gaatggaact
     5521 gcttaaacga tattctatct catttggaat tgcaggagtt ctgctttatt tttttacgat
     5581 ttgggttgag aagcaacacg agagtaatat gcctcacaat ttaacagcaa aaatcgagga
     5641 gattgtgacg attattcttc ctgtgaaacg ctattttgaa aagtgtcttg atttaagtga
     5701 atctcaggaa cttcaatatg gttttgattc tcaggctcca ttgtcgttta atcttcacta
     5761 ccatcagaag ggtgaaataa attatacgtt taaagaggac tcgattgatg aactggaaaa
     5821 cacatttcag ccggaaaaaa ggggttatta ttgtttgcaa tggggaaatt ccggaaaaga
     5881 aaaggtagaa atgaattatc atttcaatat tcttaaacgg gcttccgtca atgactgaat
     5941 gtgcagacaa tcgattttga aaagattttt gttaaattga aatagttgcg agagtggtgg
     6001 aattggtaga cacgccagat ttaggatctg gtgccgaaa
//
LOCUS       DTVD01000201            6019 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4170_length_6019_cov_383.995641, whole genome
            shotgun sequence.
ACCESSION   DTVD01000201 DTVD01000000
VERSION     DTVD01000201.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_6019_cov_383.995641"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="EYO68_06880"
     CDS             complement(<1..581)
                     /locus_tag="EYO68_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07565.1"
                     /translation="MQVASNTTTDKQTSLERQVQSKERSTTVGSKAQAVTSDGNKVVA
                     KGAERNETSQYLKVLSRIENLLIQEKLPDAAIEGFVGAIKKQIDLMSEADRQMLLILP
                     EAKTIELKSLDNLPEMITNNLRDESKSPALLKLLRQPKFAVFMRTDSKPAPKTYTAQS
                     AQTPTPAKLPTSKLVEGSPKKEKPVLTAVPEAV"
     gene            784..1578
                     /locus_tag="EYO68_06885"
     CDS             784..1578
                     /locus_tag="EYO68_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC07566.1"
                     /translation="MKRYQWNAQDYEKHSLAQQKWARELLDKLSLHGTEDVLDIGCGD
                     GKVTAEISRLVSKGTIVGIDNSSSMIALASKRYPPKDYPNLSFQQMDAGNLLFHECFD
                     LIFSNAALHWVKNQQPVIEGFFKGLRQKGKILLQLGGKGNAAEIISVLSGMQTDPEWQ
                     SYFEKFDFPYFFPGTDEYESILLKTGFKINRLELIPKDMAHAGKTGLEGWIRTTWLPY
                     TQQVPAEKRDKFVDAVSTKYIERTPVDSKGRLHVQMLRLEVEAEKK"
     gene            1633..2352
                     /gene="hisA"
                     /locus_tag="EYO68_06890"
     CDS             1633..2352
                     /gene="hisA"
                     /locus_tag="EYO68_06890"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIC07567.1"
                     /translation="MLLIPAIDLKNGRCVRLLQGEADAETVYSDDPAAMARSFEDAGA
                     KRLHLVDLDGAFRGQGTNLDSIHSILKTISIPVQLGGGLRTADDVERMLKLGVSSVII
                     GTMAVKNPDVFEDIIKHYSGEQIILGIDARNRKVAIEGWQEGTEIYDVDFALHWKKVG
                     IQRVVFTDIARDGMLSGPNMEALRNFARGTGLKIVASGGVSSMEDLELLKTLEADGVD
                     QVISGKAIYEGKLDLKEIFKC"
     gene            2346..3140
                     /gene="hisF"
                     /locus_tag="EYO68_06895"
     CDS             2346..3140
                     /gene="hisF"
                     /locus_tag="EYO68_06895"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIC07568.1"
                     /translation="MLARRIIPCLDIKDGRVVKGTSFVGLRDAGDPLELAQRYNEQSA
                     DELVFLDITASKENRGTTIELATRLARELFIPFTIGGGISSLEDMHSLVTAGADKVSV
                     NSSAIRNPNLITEGAKRFGSQCIVVAIDARKRDQNSQKQDTKDKISKWEVFSHGGSLA
                     TGLDAVAWAGEMEARGAGEILLTSMDADGHQDGFELDLTAAISGKLNIPVIASGGGGT
                     FEHFRDALTIGKADAVLAASVFHYGKYTVSEVKEYLSQNNIPIRKV"
     gene            3164..3739
                     /locus_tag="EYO68_06900"
     CDS             3164..3739
                     /locus_tag="EYO68_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07569.1"
                     /translation="MSFVYENFRLSRLISLAIFALILSSAPDASAYKEEQVRRTNKRV
                     WSIIDGKVEFLKSSYSLEKVRVSTPKPEQFQPTPQTKYERITSRQLQNMQFIPNNLKK
                     EFIRKEGRKSFATNSKGRPESFHENPNAVKKFCNIQVNTSFCIRKSVSRGKYSGNTPK
                     KHPRKKVFSHKNNKRQESKTFVKQLIRQSER"
     gene            3746..5353
                     /locus_tag="EYO68_06905"
     CDS             3746..5353
                     /locus_tag="EYO68_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIC07570.1"
                     /translation="MDQNTNNLIRLADTLSMREMDRHAIEDLGISGSVLMENAARSVA
                     DWVEENKLKNDPEAGVVVCCGKGNNGGDGFAIARLLANRGYDVSVVDAGQAKKRDAQK
                     NQSLWEKFGESASFPDTNAARLIKEADVLIDAIFGIGMEQAIEGAYREWIEIFNSNKK
                     ALRVAVDIPSGVSTDESQIFGVAAECQQTISFQTGKPGCFQHPGVKFSGNVHIADISI
                     PEIWPEQISSNQDDSVSSNLYNSIFTYLLTEEFVRDLIPVRFPDAHKGSSGHVLTVCA
                     SAGMGGAASLASSGALKSGAGLVTACVPSELRDHLPGQAPEIMTVSPKNAPEFFTEEH
                     ADFVEQLASVRDAVVLGCGLGVHSRTAKFVQQLTKHLEGPLLIDADGLNNLDEETLKE
                     RLGVAVITPHPRELSRLCGQSVTEIQDNRIATVRRLSQDWNVVLLLKGAYTVIGSPDG
                     KIFINPTGNSALATAGSGDVLSGFIGGFLAQGLSPLNATLTGAYLHGLAAECLIQKTE
                     SNNIVASDLFEGLRLARNAIKLQNYTS"
     gene            5472..>6019
                     /locus_tag="EYO68_06910"
     CDS             5472..>6019
                     /locus_tag="EYO68_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010132378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HIC07571.1"
                     /translation="MPAFKSKIDIQSADFHNNTENMGKLVDDLQEKLQKSALGGSEKA
                     RKKHSGRGKLLPRERVRLLLDPGSPFLEFSALAALDVYEDDVPAAGMITGIGRVSGTE
                     VMVVANDATVKGGTYYPLTVKKHLRAQEIALENHLPCIYLVDSGGAFLPRQDEVFPDK
                     EHFGRIFYNQANLSARGISQIAV"
BASE COUNT         1793 a         1187 c         1360 g         1679 t
ORIGIN      
        1 tgaactgctt cagggaccgc agtaagcact ggcttttctt ttttgggaga accttctaca
       61 agctttgagg taggtaattt agcgggtgtc ggtgtctgag cagactgtgc tgtgtaagtt
      121 tttggggcag gcttgctgtc ggttcgcata aaaactgcaa attttggctg ccgcagcaac
      181 ttgagcaatg ctggagattt gctttcatcc ctgagattgt tcgtgatcat ttccggaaga
      241 ttatccaaac ttttcaattc aattgttttc gcctcaggaa gtataagcag catttgccgg
      301 tctgcctcac tcattaaatc aatttgtttc ttaattgctc ctacaaaacc ctcgatagca
      361 gcatccggaa gtttttcctg gatcagcaag ttttctattc ttgagagtac ctttaaatac
      421 tgtgaagtct cattacgttc agcacctttt gctactactt tatttccgtc ggaagtaact
      481 gcttgagctt tagaaccaac ggttgtagat ctctctttac tttggacttg ccgctccagg
      541 ctagtttgtt tatctgttgt agtgttagat gctacttgca tagttaatca tgtgtgttag
      601 tgcaatttgg tttatttata attcactgaa ctagtttaat gcactttcta cgactatttt
      661 tggaattcaa tatttaagcc acagagattg ttgtgttttg atctttgctt tacgtgtaca
      721 gcttgatctc gtgtggtagt tagctgataa tggatattcg atattgtttg aaatacataa
      781 aatatgaaaa gatatcaatg gaacgcacaa gattatgaga aacattctct agctcagcaa
      841 aaatgggcca gagaactttt agacaaatta tcacttcatg gaacagagga tgtgcttgat
      901 ataggttgcg gtgacggaaa ggtaaccgcc gaaatatcca gattagtctc gaaaggaaca
      961 attgtcggta tcgataactc ttcttcgatg atagcactgg catcaaaacg atatccgcca
     1021 aaggactacc caaatctttc attccagcag atggatgccg gaaacttatt gtttcatgaa
     1081 tgttttgatc ttattttttc aaatgctgca ctgcactggg taaaaaatca acaacccgtc
     1141 attgaaggat ttttcaaagg attaaggcaa aaaggaaaga tcctcctgca gttgggaggc
     1201 aaaggcaacg cagctgaaat tataagcgta ctttccggaa tgcagacaga tcctgaatgg
     1261 cagtcatact tcgaaaaatt tgactttcca tacttttttc ctggtacaga tgagtatgaa
     1321 tcaattcttc tgaaaaccgg ctttaaaata aaccggttag agcttattcc aaaagatatg
     1381 gctcatgcag gcaagacagg cttggaagga tggattcgta ctacatggtt accatatact
     1441 caacaagtac ctgcagaaaa aagagataaa ttcgtcgatg ctgtttctac aaaatatatt
     1501 gaaagaacac cagttgattc aaaaggcagg ctccatgttc aaatgcttag gcttgaagtt
     1561 gaagcagaaa aaaaatgaat tattgtttag ataacctgct cacctcaaaa actcaaaatt
     1621 caacaccaaa aaatgctgct gattcccgca attgatttaa aaaacggacg ctgtgttaga
     1681 ctgcttcagg gagaagcaga tgcagaaaca gtatactcgg atgatcctgc agccatggcc
     1741 cgctcatttg aagatgctgg tgcaaagcgt ttgcatctgg ttgatttaga tggagcattc
     1801 aggggacaag gtacaaactt ggacagcatc cattctatac ttaaaacgat ttcaattcct
     1861 gttcaacttg gaggaggttt gcgtacagca gatgatgtag aacgaatgct gaagttggga
     1921 gtgtcgtctg taatcattgg aactatggct gttaagaatc cagatgtttt tgaagacatt
     1981 attaaacatt actccggcga gcaaattatt ctgggcattg atgcccgaaa ccgcaaagtt
     2041 gcaattgaag gctggcagga aggaacagaa atttacgatg tagattttgc cctgcattgg
     2101 aaaaaggttg gtatacaaag agtagttttt accgacattg cgcgtgacgg aatgctcagt
     2161 ggaccaaata tggaggcact gcgtaatttt gcccgtggta ccggattaaa gattgtcgca
     2221 tccggtggag tctcttcaat ggaagatctg gaactgctta aaactctgga agcagacggt
     2281 gttgatcagg taatcagcgg aaaagcaatt tatgaaggca aacttgattt aaaggaaata
     2341 ttcaaatgtt agcacgccgc ataataccgt gtctcgatat aaaagacggc cgtgtggtaa
     2401 agggtaccag ctttgtcgga ttgagggatg caggcgatcc actggaattg gcacagcgtt
     2461 ataatgaaca gtctgctgat gaacttgttt ttcttgatat taccgcttcc aaagaaaatc
     2521 ggggtacaac aattgaactt gcgacgcgct tggcacgtga attgtttatc ccttttacaa
     2581 ttggcggtgg aatctcttca cttgaagaca tgcattcact tgtaaccgct ggtgcagata
     2641 aagtttcagt aaattcttct gcaatccgga atccgaacct aattactgaa ggcgcaaagc
     2701 gttttggttc acaatgtatc gttgtggcta ttgatgcaag aaaacgtgat caaaactcac
     2761 aaaaacaaga tacaaaagac aaaatctcaa aatgggaagt cttcagccac ggcggaagcc
     2821 ttgcaaccgg attggatgca gtggcatggg ccggtgaaat ggaagcaaga ggggctggtg
     2881 aaatattatt gactagcatg gatgcagacg gtcaccagga tggttttgaa cttgatttga
     2941 ctgccgcaat cagcggaaag ctcaatatac cggtaattgc atcaggaggt ggtggaactt
     3001 ttgaacattt ccgtgatgct ctcacaatag gaaaagccga tgctgttttg gcggcatccg
     3061 tatttcatta tggaaaatat actgtttctg aagttaagga atatctcagt caaaacaata
     3121 ttccaatacg caaagtataa cactctctga gaagaacgga acaatgtctt tcgtttatga
     3181 aaattttcgc ttatcaagac tgatctcgct ggcaatattt gcgctcattt taagcagtgc
     3241 accagatgcc tccgcttaca aagaagaaca agtccggagg accaacaagc gtgtttggtc
     3301 aattattgat ggaaaagtag aatttctcaa atcgtcttat tctcttgaaa aagttagagt
     3361 atcaacacca aaaccggaac agtttcagcc aactcctcaa acaaaatacg aaagaattac
     3421 atccaggcag cttcaaaaca tgcagtttat tccaaataat ttaaaaaaag aattcatccg
     3481 caaggaaggt cgcaaatcat ttgcgacaaa ctcaaaaggt cgtccggaat cctttcatga
     3541 aaaccctaat gctgtaaaaa aattttgtaa catccaagtt aacacttcgt tttgtatccg
     3601 caaaagtgtg agtcgtggca aatatagtgg caacactccc aagaaacacc cccgcaagaa
     3661 agtctttagt cataaaaaca acaaaagaca ggaatcaaaa acctttgtta agcagttgat
     3721 acgccagtca gaacgttgac agcagatgga ccaaaatact aataatttga ttcgacttgc
     3781 agacacgctt tccatgcgag agatggatcg gcacgcaatt gaagatttag gaatttctgg
     3841 ttcggtttta atggaaaatg ccgcccgcag tgttgcagac tgggttgaag aaaataaatt
     3901 gaaaaatgac cctgaagccg gagttgtagt ttgctgcgga aaaggcaata acggcggcga
     3961 cggatttgcg attgcccgcc tgttagcgaa cagggggtat gatgtcagtg ttgtagatgc
     4021 cggacaggct aaaaaaagag atgcacagaa aaaccagtca ttgtgggaaa aatttggaga
     4081 aagtgcttct tttccggata caaatgcggc ccgactgatt aaagaagcag atgttctcat
     4141 tgatgccatt tttggtatcg gaatggagca ggcgattgaa ggtgcttacc gggaatggat
     4201 tgaaattttc aactcaaaca aaaaagcatt gcgagttgcc gtagatattc catccggtgt
     4261 ttcaaccgat gagagtcaga tttttggtgt agccgcagaa tgtcagcaaa ctatttcatt
     4321 tcaaactggc aagccgggat gtttccagca tcctggtgtg aagttttccg gaaatgtcca
     4381 cattgctgat atttctattc cggaaatttg gccggaacaa atatcctcaa atcaagacga
     4441 ttctgtttct tccaatttat ataattcgat ctttacatat ttgctgacag aggaattcgt
     4501 cagagattta attccggtac gttttccgga tgcgcacaag ggttcctccg gacacgtgct
     4561 gactgtctgc gcatctgccg gtatgggagg tgcggcaagt ctagcttctt caggtgcact
     4621 taaaagcgga gctggtttag taacagcatg tgttcccagc gaacttcgtg atcatcttcc
     4681 agggcaggct ccggaaatca tgactgtcag ccctaaaaat gcaccggaat tttttacgga
     4741 agaacatgca gattttgtag aacaacttgc ttcagtacgt gatgctgtcg tactcggctg
     4801 cggactgggc gttcactccc ggaccgccaa atttgtgcag caactgacaa aacacctgga
     4861 aggtccgctg ctgattgatg ccgacggcct caataatttg gatgaagaaa ccctgaagga
     4921 acgacttggt gtcgcagtca taactccgca tccgcgtgaa ttatcaagac tttgtggaca
     4981 aagtgtaaca gaaattcagg ataacaggat tgcaacagtt cggcgactgt cccaggattg
     5041 gaatgtagtt ctgctgctta agggtgctta caccgtaatc ggcagcccgg atggaaaaat
     5101 atttatcaat cccacaggaa actcagctct ggccactgcc ggatctggag atgttttaag
     5161 tggtttcatt ggaggttttc ttgcgcaggg actttctcct ttaaatgcaa ctttaacagg
     5221 tgcctacctt cacggccttg ctgcagaatg cttaatccaa aaaactgaat caaacaacat
     5281 tgtggccagt gatctttttg aaggcttgcg tttagcaaga aatgccatca aacttcaaaa
     5341 ttacacatca taaagcactc agcgattcaa ttgatgttaa ttttatctta gatcaaactg
     5401 gaccattttt ctagccacga cccacattct catcagctgt gacccttaat tctaaatcat
     5461 agaacctttc aatgcctgct ttcaaatcaa aaatagatat tcagtctgct gactttcata
     5521 ataacactga aaacatgggg aaattggttg atgatctcca ggagaaactc caaaaatctg
     5581 ctttgggtgg aagtgaaaaa gcacgaaaaa aacatagcgg gcgcggcaag cttttgccca
     5641 gggagcgagt acgtttgtta ctcgacccag gcagtccatt tttagaattt tctgctctgg
     5701 ctgcacttga tgtatatgag gatgatgttc ctgcagcagg aatgattaca ggcatcggtc
     5761 gtgtttccgg aactgaagtg atggttgtgg caaatgatgc aactgttaaa ggcggaacat
     5821 attatccgct gacggtaaaa aaacatttgc gggcccagga aatcgcgctg gaaaatcatc
     5881 tgccctgtat ttacctggtt gattccggag gagcgtttct tccacggcag gatgaggttt
     5941 ttccggacaa ggaacatttt ggccggattt tctacaacca ggcaaattta tctgccagag
     6001 gtatttctca aattgcagt
//
LOCUS       DTVD01000202            6000 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4196_length_6000_cov_430.461060, whole genome
            shotgun sequence.
ACCESSION   DTVD01000202 DTVD01000000
VERSION     DTVD01000202.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_6000_cov_430.461060"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            229..2730
                     /gene="lon"
                     /locus_tag="EYO68_06915"
     CDS             229..2730
                     /gene="lon"
                     /locus_tag="EYO68_06915"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIC07572.1"
                     /translation="MIQSPDSEFENENDSQEEVIEVQENIIEALPEDKSLVVSEDVFP
                     DSLLIVPLYDRPLFPKMMLPVIISEEKLEKVLLKELKGPLRYVGLVYSYEPEDDSHTN
                     EPGITENLSKVGAVGKIVQASKHGNDPMHIIVQVMDRFEIIDIASEDPVIRAKVEYIP
                     ETGEEKSEDELKAFSVSIINLIKELVQLNPLFKEELGLLMGRINLKDPATLADFAASM
                     TTASGKQLQEILETKNIKIRIEKALYLLRHELEVAKLQVKISQRVEDRMSQQQREFFL
                     REQLKEIKKELGITKEGSESETDKYEARIKKLKLSEEAEERIDEELEKLRLIEPASPE
                     YNVTRTYLDWLTILPWGIYSKDFFNIQRAARILNRDHYGLKDVKDRILELISVGIING
                     DLAGSIVLLVGPPGTGKTSVGQSIARALGRKYFRFSLGGMRDEAEIKGHRRTYIGALP
                     GKFINAIKTCKTANPVIMLDEIDKIGASFHGDPASALLEVLDPEQNKDFLDHYLDVRF
                     DLSKVLFICTANQTDTIPPALLDRMEVIRLSGYILEEKLEIARKHLLPKQLKIHGLKK
                     TQFSLPKPVLREIIDGYAREAGVRSLENNLKKLLRKTARKFVEEEIDQVKISKDDLPD
                     MLGRKAFAEESRYKKPKIGVITGLAYTSVGGATLFIEASNVEAKNPGFKQTGQLGDVM
                     VESTEIAYTFIRSLGSNDEKIKNFFAQNFVHLHVPAGATPKDGPSAGITMACALQSLV
                     AQEAIIPNLAMTGELTLTGLVMPIGGVKEKTIAAKRAGITQLIFPAENKKDFDDLDES
                     IIGKITPYFVKTFAEVLPICFPNLKGTKKHKIPKT"
     gene            2857..3684
                     /locus_tag="EYO68_06920"
     CDS             2857..3684
                     /locus_tag="EYO68_06920"
                     /inference="COORDINATES: protein motif:HMM:PF01925.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIC07573.1"
                     /translation="MHPSFFNPELLSLLGLLLVSGAFAGLLAGLFGVGGGIILVPALF
                     YVFDELGYSSELTTHLAVGTTLGIIVPTSISSAWSYYKRGSGNIVMFRKLALPVAGGA
                     LIGSLLAGNLSGSVLKAIFAVLALVIALNLFQKKQFVLGTKLPAKLKVWSVVGLIGLF
                     SAMMGIGGGVFFTSYLTAYSLPMLSAVGTSASLGLMIALPGVIGYIFSGWHSLNLPPL
                     SYGYFSLAGFLCIIPMTMVMVPQGAKLAHSLDKEKLKRWFAIFLLLMSLRMLYKIYY"
     gene            3698..4330
                     /locus_tag="EYO68_06925"
     CDS             3698..4330
                     /locus_tag="EYO68_06925"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIC07574.1"
                     /translation="MKNSRSKPFVIGISGGSGSGKSTIINEIVERLGPEKIAVLHHDA
                     YYRHRPELSFEERTIINFDHPDSLETKLLMKHLVQLISGEKVEVPIYDFAQHLRNSKT
                     KKISPCQILIVDGILVLNDSQLCELMDLKVFVDVPSDLRFIRRLDRDINERGRSVTSV
                     TEQYLVTVRPMHEKFVEPSKQNADILIPGGGNNVQAVRVLSALLQTLPAN"
     gene            complement(4359..5741)
                     /locus_tag="EYO68_06930"
     CDS             complement(4359..5741)
                     /locus_tag="EYO68_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07575.1"
                     /translation="MLNRIVFTEKNQIATLKVVDELRHVDIKPAMHALEKSISSSNSE
                     KVNLDLNNLQVISHDTQELLGLVIRHVRLQNIPITISGSSQIDPGILLSISEGRPQLL
                     EQQQQEKVPFRIKPKYFSRFSNPFFSKRPKDYTNQNLIVETEEDQNKADNQRKSGQNE
                     KQEEAENTFREVVNENYSRWLLLGWILFFSFTSVAITLLILLWPEYQSGKIYEVLLSD
                     NKSFLFEKSEVAQSSLMQTTPESSFIQAVKKENLAEVERFIDEGVDLELKDLNGYTPL
                     LHAVKQQNTELVKLLAEYGANVDTSDGLEDTPLVWASSMKNEAIVKVLLEHGADPDKG
                     NFSPLMWAAYYGNLRLVKMFLESRANLNSRTNEGWTALMWAAERGNLQVIWELLKRGA
                     SVNMQNNNGQTALMLAAGRGRTGSVGLLLNKGGDAFVVDFNKKTAADYARSKQRNEVL
                     QILEKEFELR"
BASE COUNT         1842 a         1174 c         1178 g         1806 t
ORIGIN      
        1 aatcagtaat ccagaaatgg aaatggaaaa agaataatct agagactgtt tcggtgctgg
       61 ttcatttcga ccgtatggag atgtcttaat gatattagtg tggtcacata tacaagattc
      121 ctcgcttcac tcaaaatgat atggtttaag gactttatac ggctgcatca atatttttca
      181 ataaataaaa ttcattgact acaaactaat atcaataaaa aagcatttat gattcaatct
      241 cctgattctg aatttgaaaa cgaaaatgat agtcaagagg aagtaattga agtacaggaa
      301 aacattatag aagcacttcc tgaagacaaa tcacttgtcg ttagcgaaga tgtttttcct
      361 gattccctgc taatagtacc cctttacgat cgtcctttat tccctaaaat gatgcttccg
      421 gtaataattt ctgaagaaaa attggaaaaa gttttactga aggaattaaa gggccccctc
      481 cgctatgttg ggttggttta cagctatgaa cctgaagatg attctcacac taatgaacct
      541 ggtattacag aaaatttatc gaaggtagga gccgtaggaa agattgtgca ggcctcaaaa
      601 catggtaacg acccgatgca cattattgtg caggttatgg atcgctttga gatcattgat
      661 attgcttctg aagatcctgt aattagggca aaagtggaat atataccgga aactggtgaa
      721 gaaaagtcgg aagatgaact aaaagccttt tccgtttcca tcattaatct catcaaagaa
      781 ctggtacaac taaacccttt gtttaaagaa gaactcgggc ttttgatggg acgcatcaat
      841 ctcaaagatc cggcgacact tgctgatttt gctgcatcga tgacaactgc atccggtaaa
      901 caattacagg aaattcttga aacaaaaaac ataaaaatac gaatcgaaaa agcattgtac
      961 ttgcttaggc acgaactgga agtagcaaaa cttcaggtaa aaatttctca gcgggtagag
     1021 gaccgaatgt ctcagcagca gcgagaattt ttcctgcgtg aacaactcaa ggaaattaag
     1081 aaagaactgg gaatcaccaa ggaaggttct gaaagtgaaa cagataaata tgaagcacgt
     1141 ataaaaaagc ttaaactctc tgaagaggca gaagaacgta tagatgagga gcttgaaaaa
     1201 ttacgattaa ttgaacctgc ttcaccagaa tataacgtga cacgaacata tctggactgg
     1261 ctgacaattt taccatgggg catctacagt aaagattttt ttaatattca acgcgcagct
     1321 cgaattctca atcgtgatca ctatggcctt aaagatgtca aagatagaat tttggaattg
     1381 atatctgtag gaatcatcaa tggtgatttg gcaggctcaa ttgttttgct ggttggacct
     1441 cctggtactg ggaaaacttc tgtaggacag tcgattgcca gggctttggg acgcaaatat
     1501 tttcgttttt ctctgggtgg aatgcgtgat gaagcagaaa taaaaggtca tcgtcgcaca
     1561 tacataggtg cacttccagg caaatttatt aatgcaatca aaacctgcaa aactgccaat
     1621 ccggtaatta tgctggatga aatagacaaa attggtgcaa gttttcatgg cgaccctgct
     1681 tctgctttgc tggaagtttt agatcctgaa caaaacaaag actttttgga tcactatctg
     1741 gatgtgcgtt ttgatctttc aaaagtactt tttatttgta ctgcaaacca aactgacaca
     1801 attccaccgg cactgctcga caggatggaa gtcatccggc tttccggtta catccttgaa
     1861 gaaaaactgg aaattgcacg aaaacatctg ttacctaaac aattaaaaat acacggactc
     1921 aaaaaaaccc agttttcact acccaaacca gtattgcgag aaatcattga tggctatgcc
     1981 cgtgaagctg gagtacgaag tttagaaaat aatcttaaaa aattactgag aaaaactgca
     2041 agaaaatttg tagaagagga gattgatcaa gttaagattt ccaaagacga tcttccggat
     2101 atgttaggac gaaaagcctt tgcagaagaa tcgcgctaca aaaaacctaa aattggagta
     2161 ataacagggt tggcctatac cagcgtcggt ggtgccactt tgttcattga agcctccaat
     2221 gttgaagcca aaaatcctgg atttaaacaa accggacaac taggagacgt gatggtagaa
     2281 tcaacggaaa ttgcttatac tttcatccgc tcccttggca gtaatgatga aaaaataaag
     2341 aatttctttg cacaaaattt tgttcacctg catgtaccag ccggtgccac accaaaagac
     2401 ggtccatccg caggaattac aatggcctgc gcacttcaat cactggttgc acaggaagca
     2461 attattccga atcttgcgat gacaggagag ttgacgctca caggcttagt catgccaata
     2521 ggcggagtca aagaaaaaac tattgcagca aagcgggcag gtataactca attaattttt
     2581 cctgctgaaa ataaaaaaga ttttgatgat ttagatgaaa gcatcattgg aaaaatcact
     2641 ccatattttg taaaaacttt tgcggaagtt ctgccaattt gctttccaaa tctgaaaggt
     2701 actaagaaac ataaaatccc taaaacttaa ttattgacta agagctttgt ttgcttccct
     2761 tttccgggca ccacaaagaa aataagaacg ctcttgtaaa gaaaaatact ccatttatca
     2821 gcaattttct ggttgaatac aataatctga gacttcatgc atccttcttt ttttaaccct
     2881 gaattgcttt ctttgctggg gttacttttg gtgtcaggtg catttgccgg actactggcc
     2941 ggactgtttg gagtaggtgg aggaataatt ttagttccgg ctttattcta cgtatttgat
     3001 gagttgggtt attcttcgga actcacaacc cacctggcag ttggaactac gttggggata
     3061 atcgttccaa cttcaatttc atcggcctgg tcttactata aaagaggttc cggaaacata
     3121 gttatgtttc gtaagttagc actacctgtg gctggcggtg cgttaattgg ttcattactg
     3181 gcgggaaatc ttagtggttc tgtactaaaa gccatttttg cagtgcttgc cctggttatt
     3241 gcgttaaacc tttttcaaaa gaagcagttc gttcttggca ccaaactacc tgcaaaactt
     3301 aaagtgtgga gtgttgttgg attaatcggc ctattttcag caatgatggg tattggcggc
     3361 ggagtttttt ttacttctta tttgactgct tacagcttac ccatgctaag tgctgtagga
     3421 acatctgcaa gtttagggtt gatgatagcc cttcctggag taattggata tatcttcagt
     3481 ggatggcata gtttaaacct tcctccttta agctatggtt acttcagttt agcaggattt
     3541 ttgtgcataa ttccaatgac catggtcatg gtaccacaag gagcaaaact ggctcacagc
     3601 ttggataaag aaaaactcaa acgctggttt gcaatttttc tgctgctgat gtccttgcga
     3661 atgctttaca aaatatacta ctgatgcatt tatgtctatg aagaattcca gatcaaaacc
     3721 tttcgtgata ggaatcagcg gtggttctgg ttcaggtaaa tcaacaatta taaatgagat
     3781 tgtagaacgc ttaggtccgg agaaaatagc tgtactacac catgatgcat attatcggca
     3841 caggcctgaa ttatcatttg aagagcgtac cataattaat tttgatcatc cggattcatt
     3901 ggaaacaaaa cttttaatga aacatctggt acaactgatt tccggggaaa aagttgaagt
     3961 tcctatttat gatttcgcac agcatttgcg gaattccaag acaaaaaaaa tatccccctg
     4021 ccagatattg attgtagatg gaatcctggt tctgaatgat tctcaactct gcgaattaat
     4081 ggatttaaaa gtctttgttg atgtaccttc agacttaaga ttcatcagga ggttggatcg
     4141 tgatatcaac gagcggggac gttctgtcac atcagtaacc gaacaatatc ttgttaccgt
     4201 tcgtcctatg cacgaaaaat ttgttgaacc cagcaagcaa aacgctgaca tccttatccc
     4261 tggaggcgga aacaatgtgc aagctgtcag ggttttgtct gcacttttgc aaacacttcc
     4321 tgcaaattaa agtttcagtt ccgcaaaaac agttctcatc accgcaattc aaattccttt
     4381 tcaagtattt gaagaacttc atttcgctgc ttagaacggg cataatcagc agccgttttt
     4441 ttattaaaat caactacaaa tgcatcaccg cctttattga gcagtaaacc tactgaacct
     4501 gttcttcctc ttcctgctgc cagcattaga gctgtttgac cattattatt ctgcatatta
     4561 acacttgctc ctcgttttaa cagttcccaa ataacctgta aattgcccct ctcagctgcc
     4621 cacattagtg ctgtccagcc ttcgttagtg cgtgaattca gatttgccct tgattccaaa
     4681 aacattttta ccagccgtaa attaccgtaa tatgcagccc acattaacgg tgaaaaatta
     4741 cctttatccg gatcagcacc atgttccagt aatactttta caatggcctc atttttcatt
     4801 gaagaagccc aaaccagtgg agtgtcttca agtccatctg aagtgtcaac attcgcccca
     4861 tattctgcca gcagctttac cagttcagta ttttgctgtt tgaccgcatg caacaaaggt
     4921 gtatatccat tgagatcctt cagttccaaa tcaacacctt catcaataaa tctttcaact
     4981 tcagctaaat tttctttttt taccgcctgg ataaaggaac tctccggtgt tgtctgcatt
     5041 aaagaacttt gtgccacttc acttttttca aacaaaaatg atttattgtc ggacaataag
     5101 acttcataaa ttttgccgga ttgatactcc ggccagagta aaatcaataa cgtgatagcc
     5161 acgctggtaa aactaaaaaa caggatccaa cctaacaaaa gccagcgtga ataattttca
     5221 ttaactactt cccgaaatgt attttctgct tcttcctgct tttcattctg accagatttc
     5281 cgttggttgt ccgctttatt ttgatcttcc tcagtctcaa caattagatt ctgattggtg
     5341 taatctttgg gtcttttgga aaagaaaggg tttgagaaac gagaaaaata ttttggcttt
     5401 atcctgaatg gaactttttc ttgttgctgc tgttcaagaa gttggggtct gccttcggaa
     5461 atcgacaata gaattcctgg atcaatctga gaacttccgg aaatagtaat gggtatattt
     5521 tgcagtcgta cgtgcctgat aactaatccc aggagttcct gtgtatcatg gcttatgact
     5581 tgcagattgt ttaaatccag attaaccttc tccgaattac ttgaagaaat acttttttca
     5641 agtgcatgca ttgccggctt gatatctaca tgcctcaact catcaacgac tttcaaagtc
     5701 gcaatttgat ttttttcagt gaaaacaatt cgatttagca ttttacttag tcaatgttgg
     5761 caggaaggtt gcagaatgtt tcacaggtaa ctataattgt tttctcctgt agaatcagca
     5821 aaaaacacac caagtttttg acaattttca aaagaaatat tttatgcgta aattgctgtt
     5881 ggtgctattc gttaccgtcc ttagtttgaa cacggtactt gcagaagagg atgctccggt
     5941 tttttggaaa gatcgacaga tctcccagtt ggagcaactc actccaagtt tactggactc
//
LOCUS       DTVD01000203            5965 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4242_length_5965_cov_188.520812, whole genome
            shotgun sequence.
ACCESSION   DTVD01000203 DTVD01000000
VERSION     DTVD01000203.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_5965_cov_188.520812"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            101..1522
                     /locus_tag="EYO68_06935"
     CDS             101..1522
                     /locus_tag="EYO68_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HIC07576.1"
                     /translation="MSKKTYSAGVKEYRDTYWMPEYTPKDTDILACFKITPQPGVPRE
                     EAAAAVSAESSTGTWTTVWTDLLTDLDYYKGRAYAIEDVPGDDEAFYAFVAYPIDLFE
                     EGSVVNVFTSLVGNVFGFKAIRALRLEDVRFPIAYVMTCNGPPQGIQVERDIMNKYGR
                     PLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRNRFDFVMEAT
                     LKAEKETGERKGHYLNVTAPTPEEMYRRAEHAKEIGAPIIMHDYITGGFCANTGLAQW
                     CANNGMLLHIHRAMHAVIDRNPSHGIHFRVLAKALRLSGGDHLHTGTVVGKLEGDRPA
                     TLGWIDLLRDSYIKEDRSRGIFFDQDWGAMPGVFAVASGGIHVWHMPALVAIFGDDAV
                     LQFGGGTLGHPWGNAAGAAANRVALEACVEARNQGRELEKESKDILTNAAKSSPELKT
                     AMETWKEIKFEFDTVDKLDVSHK"
     gene            1613..1966
                     /locus_tag="EYO68_06940"
     CDS             1613..1966
                     /locus_tag="EYO68_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HIC07577.1"
                     /translation="MSEVQDYPSRLSDPASRKFETFSYLPEMDNDATRKQVEYIVSKG
                     WNPAIEHTEPENAFDHYWYMWKLPMFGETDVDRILNEAELCHKANPGHHVRLIGYDNY
                     KQSQGANMVVYRAPL"
     gene            1991..2794
                     /locus_tag="EYO68_06945"
     CDS             1991..2794
                     /locus_tag="EYO68_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HIC07578.1"
                     /translation="MDDATQYLVVEEPYYRPIADEVELFEAASNAQMPMMLKGPTGCG
                     KSRFVEYMAWKQGRPLITVACNEDMTASDLVGRFLLDKDGTRWQDGPLTRAARIGAIC
                     YLDEVVEARQDTTVVIHPLTDHRRELPLDKKGELVKAHPDFHLVISYNPGYQSLMKDL
                     KQSTKQRFGALDFDYPESEIEAEIVTRESGVDAEIAGKLVEVALRSRNLKGHGLDEGM
                     STRLLVYAGQLISKGIEPIAACRLALVRPLTDDPDMRDTLDAAVHTFFD"
     gene            2819..5143
                     /locus_tag="EYO68_06950"
     CDS             2819..5143
                     /locus_tag="EYO68_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIC07579.1"
                     /translation="MSIQLDDYRELLEQTAPELKGTLDATFHEAGRYMSPVGLQDYLE
                     GARGLCELGRGADLVVSYIENVPAVAKECGEDIIRECLTAAMKLASMTSGEVIALLFS
                     SLPTAARRLGDQELMRGYLSLIHRLAAKAPRGLRPMLGCTDELLSKLTLSGLRRWSDF
                     GADAYRRDLPGQVAYFGLKTEDSRAVLKKERSGTIFINCQRKLNFYLRALWARDFFLR
                     PADTSHIGFRPYLEARVLHLPDAVDGIMVKGTELYRAITAHMAAHLVYTREPISAEHL
                     SPAQRFFVGFAEDARVEYCAKQAFPGLGKLWRTLHCQHKEKPEHASIPLLERLALALL
                     DSSFRVNDLSLDDIAEQFHANIESNKNDPQFSWHLGLEIFNALAARKDVPSLRLLECL
                     RIPYRDDNRFIWDFDEFAWHDDVEYVPANRRQVRKRASLMEFVNELDVETAGDDAQEI
                     WTLPTELFPYEDQGISFNQMEGKEPISDPFHYPEWDYQVQLHRPDWATVYERGYPMGN
                     SEDIEQILMEHKPITSRIKQLVDRLRPEGMIRQRKLEDGDEIDINAAVEAIIDIRMGN
                     QPDPRITLKNVLNRRDLAILILLDLSESTNETLAGLEKTVLELTREASALVATAISGI
                     GDAFAIHGFASDGRHDVRYYRLKRFDQAFDEEAKGRLAGMRGGLSTRMGAAMRHAGHH
                     LSLRAEKIKMLLIVTDGSPSDIDERDPQHLRMDAKKATEELHTNGIMTYCLTLDPSAD
                     HYVQRIFGQNHYTIIDNVQRLPEKLPALFASLTG"
     gene            5263..5931
                     /locus_tag="EYO68_06955"
     CDS             5263..5931
                     /locus_tag="EYO68_06955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIC07580.1"
                     /translation="MNHVKISPSILSADFSRLGEQVREAEDAGVDYIHVDVMDGHFVP
                     NITIGPVIVKALRPVTKLPLDVHLMIENPECYIEDFAKAGADIITVHQETCPHLHRNI
                     QQIHDLGIRAGVSLNPSTPVSTLDEIIAEVDLVLVMSVNPGFGGQSYISSSTGKTKKL
                     REMLDSCGSSADLEVDGGVNVDTVCEVIGAGANAFVAGSAVFNDKQSVAENVRLLREK
                     IDSA"
BASE COUNT         1539 a         1373 c         1503 g         1550 t
ORIGIN      
        1 ttccgttgac agcaatggcg gggcgttata actcaggtga attggaacaa aaaatcaatt
       61 aactttcgta ctcaacactc accttaaaaa ataaggtaat atgtcgaaaa aaacttatag
      121 tgccggtgtc aaagaatacc gcgataccta ttggatgccg gaatacacgc caaaagatac
      181 tgatattttg gcctgtttca aaatcactcc gcaacccgga gttccacgtg aagaagccgc
      241 tgccgccgtg agcgctgagt cctcaacagg tacatggaca acggtatgga ctgatctgtt
      301 gaccgacctt gattattaca aaggtcgggc ctatgccatt gaggatgttc cgggagatga
      361 tgaggctttc tatgcctttg tggcttatcc gattgattta tttgaagaag gttcggtggt
      421 taatgtgttc acgtctttag tcggaaatgt ttttggattc aaggctatcc gtgctttgcg
      481 actggaggat gtgcgttttc cgattgctta cgttatgact tgcaacggac cacctcaggg
      541 aattcaggtc gagcgtgata ttatgaacaa atacggtcgt cctctgctgg gttgtacgat
      601 aaaaccaaaa cttggattat cggctaaaaa ttatggacgt gcggtctatg aatgtttgcg
      661 cggaggtctt gatttcacca aagatgacga gaatgtcaat tcccagcctt ttatgcgctg
      721 gcggaatcga tttgattttg tgatggaagc aaccctcaag gccgaaaaag aaacggggga
      781 gcgaaagggg cattacctca acgtaacggc tccaactccg gaagagatgt ataggcgtgc
      841 ggaacacgcc aaagaaatcg gtgcgcccat catcatgcat gactacatca ccggcggttt
      901 ttgtgcaaat acaggactgg cccagtggtg tgccaataat ggtatgttgt tacatattca
      961 ccgcgccatg cacgccgtga ttgaccgcaa tccgagtcac ggtattcatt tccgtgtact
     1021 cgccaaagcc ctgcgtttat cgggtggcga tcatctgcac accggaaccg tagtcgggaa
     1081 acttgaagga gatcgtcctg ccaccttggg ttggattgat ttgttacgcg attcctacat
     1141 caaagaagat cgttcacgcg gaatcttttt cgatcaggat tggggagcca tgccgggagt
     1201 ctttgctgtt gcctccggag gaattcatgt gtggcacatg cccgcgttgg ttgccatttt
     1261 cggagacgat gctgtcctcc agtttggcgg aggaacattg gggcatccgt ggggtaacgc
     1321 tgcgggtgct gccgcaaacc gtgtggcact ggaagcctgt gttgaagcgc gtaaccaagg
     1381 acgtgaactt gaaaaagaga gcaaggatat tctcactaat gccgctaaat ccagtccaga
     1441 gctaaagact gccatggaga cctggaagga aatcaaattt gaatttgaca cggtggacaa
     1501 actggatgtg tctcacaaat aactctaaca ctttttcaaa caaggccgga ttcccgtcgt
     1561 aagactggag gctggtcgta aaaattaatc agtatctctc aaggtaaaac tcatgagtga
     1621 agtacaagat tacccatcgc gcctgtccga tcccgccagt cgtaaatttg aaactttttc
     1681 ctacctcccg gagatggata acgatgctac gcgtaaacaa gtggaataca tcgtctccaa
     1741 aggttggaat ccggccatcg aacacactga accggaaaat gcctttgatc attattggta
     1801 tatgtggaaa ttacctatgt tcggcgagac cgatgtggat cgaattttga acgaagcgga
     1861 actatgccat aaggccaacc ccggacacca tgttcgacta atcggctacg acaattacaa
     1921 gcagagtcag ggagcaaaca tggtcgttta ccgcgctcct ctataaccgt ggacaacgga
     1981 acgcagtttt atggacgacg caacgcaata tctggtagtt gaggaacctt attatcgtcc
     2041 aattgcggat gaggtagagt tgtttgaagc cgcctcaaat gcgcaaatgc ccatgatgct
     2101 gaaaggcccg accggctgcg gcaagtcccg ttttgtggag tacatggcat ggaaacaagg
     2161 tcggcctctc attacggttg cttgcaacga agacatgacc gcttcagatt tggtgggccg
     2221 cttcctcctg gataaggacg gaacccgttg gcaagacggc cctctgactc gggccgcccg
     2281 catcggtgcc atctgctacc tggatgaggt agtggaagcc cgacaggata caaccgtggt
     2341 cattcatcct ctgacagacc accgccgaga gttacctctg gataaaaaag gcgaactggt
     2401 caaagcccac cccgactttc atctggttat ctcctataac cccggatatc aaagcctgat
     2461 gaaagacctc aagcaatcca ctaaacaacg tttcggtgct cttgatttcg attatccgga
     2521 atccgaaatt gaagctgaga tagtgactcg cgaatccggt gttgatgcag aaatcgccgg
     2581 gaaactggta gaggtggcct tgcgctctcg caacctcaaa ggtcacggtc tggatgaggg
     2641 catgtccacg cgcttactcg tttatgcagg ccagttgatc agcaagggaa ttgagccaat
     2701 tgctgcttgc cgcctggctt tggttcggcc gctgacggat gatccggaca tgcgggacac
     2761 tcttgacgct gcagttcata cgttcttcga ctaaattcac aaaaacaaag gtcggtgcat
     2821 gagcatccaa cttgatgatt atagggagct cttggaacaa actgctcctg agttaaaagg
     2881 gactctggat gcgacgtttc acgaagcagg acgctacatg tctcctgttg gacttcaaga
     2941 ttacctggag ggtgcccgcg gactatgtga attgggtcgg ggggctgatt tagtggtaag
     3001 ctatattgaa aacgtgcccg cagtggctaa ggagtgcgga gaagacatca ttcgtgaatg
     3061 cttaaccgcc gccatgaagc tggcttcaat gacttcgggt gaggtgatcg ctctcttatt
     3121 ttccagtctt cctactgccg cccgccgttt gggagaccag gaactaatgc gcggatatct
     3181 cagcctaatc catcgattag ctgccaaggc tccacgtggc ttgcgtccca tgctgggttg
     3241 cactgatgaa ttgctttcca agctcacttt gtctggcctg cgtcgctggt cagatttcgg
     3301 tgctgatgcc taccggcgcg accttcccgg acaagtagcc tattttggtc tgaagactga
     3361 agacagccgc gctgtcttga aaaaagaacg cagcggtacc atttttatca actgccagcg
     3421 taaactcaat ttttacctgc gtgctttgtg ggcaagggat ttctttttgc gtcctgctga
     3481 cacgagtcac atcggattca gaccatatct tgaagcccgt gtacttcacc tgccggatgc
     3541 agtggatggt ataatggtta aaggtacaga gctttatagg gctattactg cacacatggc
     3601 cgctcatttg gtatataccc gagagccgat ttcagccgaa catttatctc cggcccaaag
     3661 atttttcgta ggttttgctg aggacgccag ggttgagtat tgcgcgaaac aagcatttcc
     3721 cggtcttggt aaactctggc gtactctgca ttgtcaacac aaggaaaaac ctgaacatgc
     3781 atctattcca ttgcttgaac gcttagcttt ggcattgtta gacagttcgt ttcgtgtgaa
     3841 cgatcttagt ctggatgaca ttgctgaaca gttccatgca aacattgagt ccaataaaaa
     3901 cgacccgcag ttttcctggc atttaggtct tgaaattttt aatgccctgg ctgcacgtaa
     3961 agatgtcccc agtctgcgtc tccttgaatg tctccgaata ccctaccggg atgacaaccg
     4021 ctttatttgg gattttgatg aatttgcctg gcacgacgat gtggaatatg tgcccgcaaa
     4081 ccgacgacaa gtacgtaagc gtgccagtct gatggaattc gtcaatgagt tggatgttga
     4141 gactgccgga gatgatgcgc aagaaatctg gactttgccc acggaactct ttccgtatga
     4201 ggatcaggga atcagtttta accagatgga gggcaaggag ccaatcagcg atccttttca
     4261 ttatcctgaa tgggattacc aggtacagtt gcaccggcca gactgggcca cagtctatga
     4321 acggggttat cccatgggga actcggagga catagagcag atattgatgg aacataagcc
     4381 gatcacctcc cgcatcaaac agcttgttga tcgcctgcgt ccggagggta tgattcgtca
     4441 gcgcaagttg gaagatggag acgagattga catcaatgct gccgtggaag caattatcga
     4501 tattcgtatg gggaatcaac ctgatccaag aattaccttg aagaatgtgc tcaaccgccg
     4561 tgatctggct attcttattt tgcttgacct ctccgaatcg acgaatgaaa cactggcggg
     4621 attagagaaa acggtgttgg aactgactcg ggaagcaagt gctttggttg ccacggcaat
     4681 ctcaggaatc ggtgacgctt ttgccattca tggttttgca tctgatggac gacatgatgt
     4741 acgttactat cgcctgaaac gttttgatca agcttttgat gaagaagcta agggtcgtct
     4801 cgcggggatg cgcggaggtt tatccacccg catgggagcg gccatgcgcc atgcaggaca
     4861 ccacctttcg cttcgtgctg aaaaaataaa aatgttactg attgttacag atggatcacc
     4921 ttccgatatt gatgaacggg atccacaaca tttgcgtatg gatgccaaaa aagcaactga
     4981 ggaactgcac acaaacggca ttatgaccta ctgtctgaca ctggatccga gcgcggatca
     5041 ttacgtccaa cggatttttg gacagaatca ttataccatc atcgacaatg tccagcgctt
     5101 gccggaaaaa ctaccggcac tttttgcatc gttgacaggc taatcaaaaa aaacttgatt
     5161 gttattcccc gaaatactga aactgtgtac tctattccct gtaatatagg attacttagg
     5221 tctggtgttt cgtaactctt aactcttcat aaggtgtgta ctgtgaatca cgttaaaatt
     5281 tccccttcca ttctatccgc agatttttct cgtctaggtg aacaagtccg tgaagctgaa
     5341 gatgcagggg tagattatat ccatgtagat gtaatggacg gacattttgt tcccaacatc
     5401 acaattggcc ctgtgatagt gaaagccttg cgtcccgtta ccaaactgcc gctggatgta
     5461 cacttgatga ttgaaaatcc ggaatgttat attgaggatt ttgccaaagc cggagctgat
     5521 ataattaccg tgcatcagga gacatgtcca cacctgcatc gtaacattca gcaaattcat
     5581 gatttaggaa tccgtgccgg tgtcagtctc aatccctcca cccctgtgag tacattggat
     5641 gaaattatcg cagaagtaga cctggtactt gtgatgtcag tcaatccagg atttggcgga
     5701 cagtcctaca tttcttccag tacaggcaaa acaaaaaaat tacgcgagat gcttgactca
     5761 tgtgggtcgt ctgcagattt agaggtggat ggcggcgtta atgttgacac agtttgtgaa
     5821 gttattggcg ctggagccaa tgcctttgtt gccggctccg ccgtttttaa cgacaaacag
     5881 agtgtagcag aaaatgtacg tcttcttcga gaaaaaatag attctgcctg aatgttcaag
     5941 gtttcctttt tatccagctt ttaaa
//
LOCUS       DTVD01000204           20699 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_424_length_20699_cov_223.576439, whole genome
            shotgun sequence.
ACCESSION   DTVD01000204 DTVD01000000
VERSION     DTVD01000204.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_20699_cov_223.576439"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(82..1020)
                     /locus_tag="EYO68_06960"
     CDS             complement(82..1020)
                     /locus_tag="EYO68_06960"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HIC07581.1"
                     /translation="MPKNRVDRPIILGIVGDSAAGKTTLAAGVAQILGKDQVVTICTD
                     DYHKFDRAKRAENKTSALDPECNYLDVIENDLNSLRNGKAIIKPVYSHHDGCFKAAEY
                     IEPKPYIVVEGLLGYSTRTMRNNYDVKIYLDPEEELRVRWKVIRDTTKRGYTEEQVLA
                     SLEKRKDDSPAYIQPQRTFADMAIQFYRPEGMEDELGPGLNVRHTLRPTLPHPDLTSL
                     LDVGENTGISLDLARDKDGKPVDILDIHGSIETHRASKLEELLWGLIPEAQHLRANKR
                     SIEELGKIIIISHPLMLTQMLIAYHMVKAALGHHAI"
     gene            complement(1129..2088)
                     /gene="glpX"
                     /locus_tag="EYO68_06965"
     CDS             complement(1129..2088)
                     /gene="glpX"
                     /locus_tag="EYO68_06965"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIC07582.1"
                     /translation="MDSVVTHDLIFPYLLRKVTEKAALASYNWIGRGNKIGGDSAAVE
                     AMRASLEELKINGTIVIGEGEKDEAPQLYNGEVFGNKDASDQLDIAVDPLEGTTFLAD
                     GQTNAMAVIATAPKGTMFDPGPAFYMEKFAAPAETRGKIDMDWPIEKKLLTVAKLIEK
                     DIENLTVFVLDKPRHRSLIEEIHHAGARVSLYPAGDIAGALMAAIPDSEVDVLMGTGG
                     TPEGIISASAIRAMGGEFLGRLDPQLATELVRVRDADLDTKRWYDRDELIGSDDVHFC
                     ATGITSGMLFSGVNQTKHHFRTETLIVTGTRKEMVSLTNWHRR"
     gene            complement(2244..3497)
                     /gene="purD"
                     /locus_tag="EYO68_06970"
     CDS             complement(2244..3497)
                     /gene="purD"
                     /locus_tag="EYO68_06970"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIC07583.1"
                     /translation="MDILVIGGGGREHAIVWKLNNRGHNIFCAPGNPGIADIAECVSL
                     QVNQMKELADFAQSNKIDLTVVGPEDPLTNGIVDQFEKRSLLIFGPNQESAHLEGSKA
                     YAKKVMERFNVPTAAYMEFDDFEEALSYVEEQGFPIVIKADGLAAGKGVTVAETIEEA
                     ETALQAAMLQKIFGESGTKVVIEECLLGEEMTVLAFVDGKTVLPMVPSQDHKPVFDGD
                     RGPNTGGMGAYSPVPHLEKWIPEIEKNIIRPVAEGLAAEGTPYRGILYTGLMLTENGP
                     KVIEFNVRFGDPEAQVILPLLENDLAEVLLAVAEKRLHEIDLSWKPGASVCVIAAAPG
                     YPGPPIKGLPISLPKNFTAEKQIFHAGTALQNEQLVTNGGRVFGVTSQGTNIAEARQR
                     AYSLMEGVSFKGKHFRRDIASKALN"
     gene            complement(3498..4067)
                     /gene="purN"
                     /locus_tag="EYO68_06975"
     CDS             complement(3498..4067)
                     /gene="purN"
                     /locus_tag="EYO68_06975"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIC07584.1"
                     /translation="MRIAVFASGRGSNLLAIHDAVISGKLEGVLLDLAVCDRPDAPVV
                     DLVHKRNIPDFIFCPKDYADKNAFETVIAEKLAEAKIELVVLAGYMRLVGAVLLEKWE
                     GRMINLHPSLLPAFPGKDAIGQAWRAGVKETGVTVHFVNAGMDTGRIYLQQKVTVDPD
                     DTEESLSEKIHLLEHKLLPDAILKIMQEM"
     gene            complement(4070..5125)
                     /locus_tag="EYO68_06980"
     CDS             complement(4070..5125)
                     /locus_tag="EYO68_06980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIC07585.1"
                     /translation="MKAYQKAGVDLAAADEAVEKLKPLAKRTLRPEVLSGLGGFGGLF
                     ELNQSKYKNPVLVSGTDGVGTKLKLAFELDRHDTIGIDLVAMCVNDILVQGAEPLFFL
                     DYLACGKLLPERVEDIVSGISEGCLESGSALLGGETAEMPGMYDSGEYDLAGFAVGAV
                     ERKKLIDGKRIEIGDLLLGLPSSGVHSNGFSLVRKILKDHKLNLKDILPGTTETLGEA
                     LLTPTLIYVKPILKLLAEIEIIGMSHITGGGLPGNVPRMLPEKTPEGFVPVAEIEKSE
                     HYNSWPELPIFSLLQQVSGLPDEELFPVFNMGIGFVICVAETNEGIAREILTKEGQPV
                     FRIGQIVSGNKPEVCWK"
     gene            complement(5162..6511)
                     /locus_tag="EYO68_06985"
     CDS             complement(5162..6511)
                     /locus_tag="EYO68_06985"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIC07586.1"
                     /translation="MCGIFGVFNHTQAAELTYFGLHSLQHRGQESAGICVSDGEKFHT
                     HRGTGLVTEAFGQDQHLQLAGHIGIGHVRYTTAGDSGLVNAQPLVFRYTGGQVAVAHN
                     GNLVNAKRLRNQLERQGSIYQTNSDTEVVPHLIARSGPPIEQAIPDALRVIEGAYALL
                     FMTETQLVAAQDPRAFHPLALGKLDEGWVVASETCALDVCGAEFVREIEPGEMLVIDT
                     EGLHSQTFMQPSSRSICSFEYIYFARPDSEIDGISVHNVRKEMGRQICREVPTEADVV
                     IGVPDSGLAAAIGFAEESGLPYEMGMMKNRYVGRTFIQPNQELRRKGVRMKLSVVRKA
                     VEGKRVILVDDSIVRGTTSGRIVELLKAAGATEVHMRVTSPPITHPCLYGIDTARRME
                     LISATQTPEEICQKIGADSLHFISAEGMISATDRNDSTRDRGHCLACFNGNYPTPTC"
     gene            complement(6664..8961)
                     /gene="purL"
                     /locus_tag="EYO68_06990"
     CDS             complement(6664..8961)
                     /gene="purL"
                     /locus_tag="EYO68_06990"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIC07587.1"
                     /translation="MHPDEPTPDQIHCKKVYKQMGLNDDEYSLVVELLGRRPNLTETG
                     IFGVMWSEHCSYKNSKSLLSRFPVDGPKVLQGPGEGAGVVDIGDGYAVVFKVESHNHP
                     SAIEPYQGAATGVGGILRDIFSMGARPVAVLNSLRFGPLSDSEADKNSISRTRYLFGN
                     VVAGIAGYGNCIGVPTVGGEVCFDECYTGNPLVNAMGVGILRHDQLQHGRAEGIGNSV
                     IYVGAATGRDGIHGATFASEELNEESDAKRPAVQVGDPFMGKLLIEACLEVYESGAIY
                     SVQDMGAAGLTCSSTEMSDKGGVGMEMNLNLVPQRAENMSAYEIMLSESQERMLFVTN
                     PGREEEVFAIFKKWDVPAVTVGHVIEEQVLRLKHHGEKVAELPLNTVCGASPVYQRES
                     KMPQEVIERQQQTAITWGVPENLQIVFQQLIQTPELASKSWVYQQYDFMVRTSTSVRP
                     GSDAGVVRVEGTDKALAMSIDGNSRYVFLDPKTGGKIAVAEAARNVVCSGAHPLAITD
                     GLNFGNPEKPEIFWQLEQAVSGIAETCNFLKLPVTGGNVSLYNETEGEAIYPTPVVGV
                     VGLIEHNDHTTTQFFQNSGDVLLLLGDTKNELGGSSYQQMQLGKITGMPPELDLDKER
                     TLQQTLLEIIRSGIVNSAHDLSQGGLALALLTSCTENFGILVNWEIDLRPDIALFSES
                     QSRILLSISPDNTELVEVVMQKKGVPCARIGKVVSSGTSGSNCGGSTGRFDISLNGTP
                     VIQAELNPLRVLWKNAIGREMEISV"
     gene            complement(9050..9712)
                     /gene="purQ"
                     /locus_tag="EYO68_06995"
     CDS             complement(9050..9712)
                     /gene="purQ"
                     /locus_tag="EYO68_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIC07588.1"
                     /translation="MRCAVIVFPGSNCDFDLYKAANYIEGIQAEYVWYTSESLDGFDA
                     VLVPGGFSYGDALRAGALAARAPILKALQRAADAGTRVLGICNGFQILTECGLLPGAL
                     HRNSGLRFICKTVNLEVSNTATSFTTGYAGRTSTHVRFGIAHGEGNYTCDSDTLQKLE
                     DNGQIVFRYSGDNPNGSVANIAGITNEAGNVLGMMPHPERAMADWMGSTDGRILFESM
                     RN"
     gene            complement(9712..9960)
                     /gene="purS"
                     /locus_tag="EYO68_07000"
     CDS             complement(9712..9960)
                     /gene="purS"
                     /locus_tag="EYO68_07000"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIC07589.1"
                     /translation="MLKGIVTVSLKQDVLDPQGQAVLHSLQTLGFPEAQNVRIGKSIE
                     VWLEESDSQKAESRLKEMCEALLVNSVIEDYSFEIVED"
     gene            complement(9985..10692)
                     /locus_tag="EYO68_07005"
     CDS             complement(9985..10692)
                     /locus_tag="EYO68_07005"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIC07590.1"
                     /translation="MSGTLLYEGKAKKIFSSDDPNIVRVAYKNDTTAFNGEKFEKLEG
                     KGQLNNEITTFFFKYLAEQGIASHFVRKISDTEQLVKRVEIVPLEVVTRNFSAGSLSK
                     RLGWEEGRKLPEPIVEFYYKDDELGDPLLADVHIHTLGLATDEELNELRKRALEVNDC
                     LLKLLLPRGLLLVDFKLEFGRDSSGNLLLADEISPDTCRFWDAETREKLDKDRFRRDL
                     GGVTEAYAEVLRRLNSS"
     gene            complement(10780..11940)
                     /gene="purK"
                     /locus_tag="EYO68_07010"
     CDS             complement(10780..11940)
                     /gene="purK"
                     /locus_tag="EYO68_07010"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIC07591.1"
                     /translation="MNLDPEPLQPGATIGLLGGGQLAQMLVLAGHAMGFRFMVLEPNP
                     DCPAALAGAEQITASYTDPEALAKLADSCQLVTYEFENVDAEAVSWLEERVDVPQGSE
                     ILRIAQNRLREKKTLVRLNIPVAPFQKITTENELSLALKSLGELMLKTVTGGYDGKGQ
                     VRISKVEQIPEAFSTLYSTKTEIVAEQFVSFEREVSVIVARNRLGEISCFPVAENVHE
                     KNILTISRIPARLDSRVREQAQLLAKQIAEGLELVGVLAVEMFVLPDGKLMVNELAPR
                     PHNSGHFTLNDCATSQFEQHLKAITNFPLGSTEMYKPAIMLNILGEHLPLLLKRLKDL
                     PPEAKLHLYGKQECRPGRKMGHLNLTADSPESLLEPLVKLGIWDEALLYKML"
     gene            complement(11937..12437)
                     /gene="purE"
                     /locus_tag="EYO68_07015"
     CDS             complement(11937..12437)
                     /gene="purE"
                     /locus_tag="EYO68_07015"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIC07592.1"
                     /translation="MAQKKVNYPLVGVIMGSTSDWETMREACEVLDELNVSYEKRVVS
                     AHRTPEWMSAYATQAEERGLQIIIAGAGGAAHLPGMVAAQTLLPVIGVPVKSSTLNGQ
                     DSLLSIVQMPRGVPVGTVAIGGAGAHNAGLLAAQILANNDPELRKRLIRRRERVRKTV
                     LEAELP"
     gene            complement(12458..13642)
                     /locus_tag="EYO68_07020"
     CDS             complement(12458..13642)
                     /locus_tag="EYO68_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC07593.1"
                     /translation="MSIANSIRQSIEKGSWIRQMFEAGARLKAEHGSENVFDFSLGNP
                     ILEPPEEVHVKLRQLLENPEPGMHRYMPNAGFAETREYVAGVMQDETGHPFESEDVVM
                     CVGAGGGLNVVFKALLEPGDEVLALAPFFVEYGAYAQNHGGCLKTAQTTAEFQPDLNS
                     IEVSLSERIKIVIINSPNNPTGAVYSQETLDKLGQLLLHKEKEFGHPIYLVSDDIYRY
                     LVFDGLTNCNAFLSYPNSILVNSHSKDLGLPGERIGYIAIHPDIIEREELRAALVLCN
                     RILGFVNAPAMMQKILPLLGNARVDISVYERSRDKIMSMITDLGFEAVKPQGAFYIFP
                     KSPDSDDVAFVKRAQQENILLVPGTGFGGPGHFRISLCCTQETIENSRPGFVRLAEHY
                     GL"
     gene            complement(13760..14875)
                     /gene="dnaJ"
                     /locus_tag="EYO68_07025"
     CDS             complement(13760..14875)
                     /gene="dnaJ"
                     /locus_tag="EYO68_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIC07594.1"
                     /translation="MAKRDYYEVLGVSRNASSDELKKAYRKVAMKHHPDRNPGDKKSE
                     EKFKEASEAFEVLGDKEKRSRYDQFGHAAEGMGSGMGGFGAAGGSGFGDVFGDIFSEF
                     FSGSPGSTASRGERGSDLQYNMEISFEDAVFGSSKEIDVPRMETCDSCSGLGAESEKD
                     IKICGTCGGTGQQRVQQGFFSVATTCSSCGGRGKTISNPCKTCQGRTRVSKTKRIRVN
                     IPAGVSSGSRIKLTGEGEHGVNGGSSGDLYIVLSVKDHSIFERDGADIFCEVPISFAQ
                     ATLGTDLEVPTLEGKARLKIPAGTQTHKIFRLKNQGIAHLRGGGRGDLHVRIVVETPT
                     KLSERQIELLKEFDDCCDDESNPIRDKFVKSIKNLFT"
     assembly_gap    14897..14996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15073..17004)
                     /gene="dnaK"
                     /locus_tag="EYO68_07030"
     CDS             complement(15073..17004)
                     /gene="dnaK"
                     /locus_tag="EYO68_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIC07595.1"
                     /translation="MGKVIGIDLGTTNSCVSIMEGGEPKVIPNAEGTRTTPSMVAFND
                     EGERLVGQVAKRQAVTNPLRTIYSIKRLVGQEYKSAEAKHTKKMVSYEVVKGNQNLAY
                     VKVDDKNYSPQEISASILQKMKQTAEEYLGESVKEAVITVPAYFSDAQRQATKDAGKI
                     AGLEVLRIINEPTAAAMAYGLDKKHDEKIAVFDLGGGTFDISILEIGEGVFDVKATNG
                     DTFLGGDDFDNRLISYFADEFKKDNGIDLREDKMALQRLRENAEKAKHELSSSSETDV
                     NLPFITADASGPKHLNLKITRAKFESLAEDLVNRCLEPCRKALKDAGCTAKDINEVIL
                     VGGMTRMPKVQEMVKEFFGKEPHKGVNPDEVVAIGAAIQGGVLTGDVKDVLLLDVTPL
                     SLGIETLGSVMTKLIERNTTIPTRKSQTFSTAADNQPAVSIHVLQGEREMSSDNKTLG
                     RFELVGIPPAPRGMPQIEVTFDIDANGIVHVSAKDLGTGKEQAIKIESSGGLSEEDVE
                     KMINDAEQFADEDKAKRESVEVKNQAETLIYSTEKSISDLGDKLPEEDKTKIEACSEQ
                     LKKSMETDDMEAIKSDLEALTQASHKMAELLYSQQAEQQGDGSGEGSEDAPGNDEKSG
                     KDDDDIVDADFEEVKKSDK"
     gene            complement(17110..17718)
                     /gene="grpE"
                     /locus_tag="EYO68_07035"
     CDS             complement(17110..17718)
                     /gene="grpE"
                     /locus_tag="EYO68_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIC07596.1"
                     /translation="MSARKLKETAVKKQKTNKPKKEKANGNIEEVTAAEISGTSADNK
                     ETASAEEDLLEKERIRAQNMEDRFLRVNAEFENYKKRMIRESSDRLKYFHLNFIKELL
                     PSLDNLERAISHAKSENNDVDSMIEGLEMVNKMTHEVFEKFGVSRVDTIGEVFDPNFH
                     QAVGVVESDSVPENHIVEECLGGYLLHDRIIRPAMVRVSGKN"
     gene            complement(17737..18822)
                     /gene="hrcA"
                     /locus_tag="EYO68_07040"
     CDS             complement(17737..18822)
                     /gene="hrcA"
                     /locus_tag="EYO68_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HIC07597.1"
                     /translation="MGKKAAVTGKRGLLEDLVLNERAQLVLNSIIENYIQSSEPIGSR
                     TLSKSIGVTLSPATIRNIMSDLAELGFLIQTHTSAGRVPTDKAYRFYVNSLSTPSSVP
                     EQIKQKITLVSTEQGMQVEGILAEAVHLLADLTKFACVVSTPKASVSRLQRIELIKIS
                     EDRILVILVTKAGVVRDKIIFVKDSHSQDFLNSVASFLNEKFKNHSMQEIREKIHQYL
                     VDDRNRYHDLLAQAVRLGKKAFELNNPGEMLIDGQMNLLLDSHFHKQSSVRSLIEAFN
                     QKSEIMKILDDSMRQKGVHIFIGIENDFEQLQGCSLIMTSYRNNQNVLGSIGVVGPTS
                     MDYKRIISIVEYTAKILSETITERSYD"
     gene            complement(19025..19378)
                     /locus_tag="EYO68_07045"
     CDS             complement(19025..19378)
                     /locus_tag="EYO68_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07598.1"
                     /translation="MNKISEDKIKENWPNAVEGDLEHPELGFIHYWTGEQRGRIVVRF
                     SYTDQEEGESKKMFFIDLSKEGWILRHISTFQSQDSKLKLVKNQSFREQDELEQKYRG
                     IIDLFLESRKLRNHL"
     gene            complement(19385..20581)
                     /locus_tag="EYO68_07050"
     CDS             complement(19385..20581)
                     /locus_tag="EYO68_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07599.1"
                     /translation="MHESVGLSLLRKICQFPPSLTVGVKENSWVRSPAWDVFWLHSGL
                     WLTFLMLTTSDLPVQEMFYAAGVFLFWISHRFSSFYLAWRTQAYHPLLKNQQGRFVFI
                     PLLLVSVVFAVLFIPESVLPVPVSVRILGMILLDFAWGMHHFAAQHYGLLRLYHHRWN
                     SKSAPLSNKRDRWYCWGVGGGLVLIAELLHGTSFLQEKNFLPLLTGDWVFEGIPLLLR
                     LGTLLVAGITIFMIRNAFLHDSGLPRILYILGIGVMVMGAYQLEPFQFFMLWTLQHWM
                     VALGLTVHMAGNDITLKTNPEIGFTGDSSVDIFWKPWKVLFLLCTFSVLMTPFLEIEA
                     VSAGGRYSEQFFPSFMEWLQNSSWERFLVGIGLASGFMHYFMDRAVYRFSDPETRKSA
                     QQLLFV"
BASE COUNT         5636 a         5046 c         3889 g         6028 t
ORIGIN      
        1 gagaaacagt catagtgaaa tcaattagct ataaataatt tatacaaaag ttgtccggtt
       61 tctattttta ttttctagtt tctagattgc atgatgtccc aatgcagctt tcaccatatg
      121 ataggcaatc agcatttgag tcagcatcag aggatgactt atgataataa tttttcccaa
      181 ttcttcaatg gaacgtttat ttgcacgcaa atgttgtgct tctggaatca aaccccacaa
      241 cagttcctca agtttacttg cgcgatgagt ttcgatacta ccatgtatat caagaatatc
      301 gactggtttc ccatctttgt cacgtgccaa atcaagggag attcctgtat tctctccgac
      361 atccagcaaa gaagtaagat caggatgcgg caaggtgggc cgtaaagtat gccggacatt
      421 gagtcccggc ccaagttcat cttccatgcc ttccggtcga taaaactgaa ttgccatatc
      481 ggcaaaagta cgctgcggct gaatgtatgc cgggctgtca tctttcctct tttccagaga
      541 ggccaaaacc tgctcctccg tgtaaccgcg ctttgttgta tcccgaatca ctttccagcg
      601 tacacgcaac tcctcctccg gatccaaata aattttcaca tcatagttgt tgcgcatggt
      661 cctcgtactg taaccaagca aaccctccac tacaatgtag ggtttcggtt caatatattc
      721 agcagcttta aagcaaccat catgatgact gtataccggt ttgatgatgg ctttgccgtt
      781 gcgtaaacta ttcagatcat tttcaataac atcaagatag ttgcattctg gatcaagcgc
      841 agatgtctta ttttcggcac gttttgcacg atcaaactta tgataatcgt ctgtacatat
      901 tgtcacaact tgatcttttc cgagtatctg ggcaactccg gcggcaagag tggttttacc
      961 tgctgcagaa tctcctacaa tccccagaat tatcgggcga tcaacacggt ttttcggcat
     1021 ggtttttctc gtttatagat ctagtttcaa taaataatgc aaacgtctgg gagcaagaca
     1081 aagcgcgcct ttttcatttt tgccttttgc actaatattc agtttgcctc agcgcctgtg
     1141 ccaattggtt agcgacacca tttcttttct ggtgcctgtc acaattaaag tttccgtccg
     1201 gaaatggtgt ttagtttgat ttactccact aaataacatt ccagaagtaa ttccggtggc
     1261 acaaaaatga acatcgtccg acccaatcaa ctcatcacgg tcataccagc gcttggtatc
     1321 caaatctgca tccctgaccc gaacaagttc agtagcaagc tgcggatcaa gccttcccag
     1381 aaattctcct cccattgccc gaatcgcact agcagaaatt atcccttccg gagttccacc
     1441 ggtacccatc agtacgtcta cttcagaatc cggaattgcc gccattaaag cgccggcaat
     1501 atcacctgca ggataaagtg aaacacgagc accggcatga tgaatctcct caataaggct
     1561 tcgatgtctt ggcttgtcta aaacaaaaac cgttaaattt tcaatatctt tttcaattaa
     1621 cttggcaacc gtgagtagtt ttttctctat tggccaatcc atatcgatct tgcccctggt
     1681 ttctgcaggt gctgcaaatt tttccatata aaaagcaggg cccgggtcaa acattgtccc
     1741 ttttggtgcc gtagcaatga cagccattgc gttggtttga ccgtctgcta aaaatgtcgt
     1801 tccttctaag ggatcaacgg caatatcaag ttgatctgac gcatccttgt ttccaaatac
     1861 ttcaccattg tataattgag gtgcttcgtc tttttcccct tcaccaataa caattgttcc
     1921 gttaattttt aactcttcca aagatgcccg cattgcttca actgcagcac tgtctccccc
     1981 aattttgttg ccgcgtccaa tccaattata tgatgccagt gcagcttttt ccgtaacctt
     2041 tcgaaggaga tagggaaaaa ttaagtcgtg ggtaaccaca ctgtccataa gggtttttct
     2101 agaacaaggt tcagctaagt tatttaatgt ctatgcaatt tactataaaa tgattattga
     2161 gacaaccctc taaaaggtgg ggaatgcatt tcttttaggt aggtaaatac taccctaatg
     2221 agtagtggaa cataaacagt tttttagttc aatgctttgg aagcaatatc acgccgaaaa
     2281 tgtttccctt taaaacttac cccttccatc agcgaatagg ctcgctgcct ggcttccgca
     2341 atgtttgtgc cctgagaagt aactccaaaa acacgcccac cgtttgtaac gagctgctca
     2401 ttctgtaacg ctgtgcctgc atgaaaaatt tgtttttctg ctgtgaaatt tttcggcagt
     2461 gaaattggca aacctttgat gggtggtccg ggataacccg gtgcagcagc gattacacaa
     2521 actgaggctc ccggtttcca ggataagtct atttcatgaa gtcgtttttc tgcaactgcc
     2581 aataatactt ctgccaaatc attttccaga agtggcaaaa ttacctgcgc ttcaggatca
     2641 ccgaaacgta cgttaaactc tattactttc ggtccatttt cagtaagcat caagcccgtg
     2701 tagagtatac cccggtaagg tgttccttct gcggccaaac cttcagcaac aggccgaatg
     2761 atatttttct caatctcagg aatccatttt tcgagatgtg gaactggtga ataggctccc
     2821 attcctcccg tgttcggtcc tcgatctcca tcaaaaaccg gtttgtgatc ttgtgacgga
     2881 accatcggca atacagtctt tccatcaaca aaagccaaaa ctgtcatttc ctcaccaagc
     2941 aaacattcct cgatcacaac ctttgttccg gattcaccaa atattttctg gagcattgca
     3001 gcctgcagtg cagtctctgc ctcttcaatt gtttctgcaa cagtcactcc cttaccggca
     3061 gctaatccat cggctttgat tacgatcgga aatccctgct cttcaacata ggacaaggct
     3121 tcctcgaaat catcaaattc catatacgct gcggtaggaa cattgaaacg ttccatcact
     3181 ttctttgcat atgccttgct gccttccaaa tgtgcagatt cctgatttgg accaaaaatt
     3241 aacaggctgc gtttttcaaa ctgatccaca atcccgtttg tcaacggatc ttccggacca
     3301 accacggtta aatcaatttt gtttgattga gcaaaatccg ccagttcttt catttggttt
     3361 acctgcaaag acacacattc tgcaatatct gcaattcccg gattgccagg agcacaaaaa
     3421 atattatgtc ctctgttatt aagtttccag actatagcat gttcacgacc tccgccgccg
     3481 ataaccaaaa tatccattta catttcctgc ataattttca gaattgcatc cggcaacaat
     3541 ttatgttcca gcagatgaat tttttcggac aaactttctt ctgtatcatc cggatcgact
     3601 gttacttttt gttgcaaata aatacgaccc gtgtccatac cagcattaac aaaatgtact
     3661 gtcacaccag tttctttaac tcctgcacgc catgcctgac caatggcatc ttttccggga
     3721 aatgccggca gtagagatgg atgcagattt atcattcgcc cttcccattt ttccagcaaa
     3781 acggctccta ccaaacgcat ataacctgcc aaaacaacaa gctcaatttt tgcttcagcg
     3841 agcttttccg caatgactgt ttcaaatgca tttttgtctg cataatcttt gggacaaaaa
     3901 ataaagtccg gaatatttct tttatgcact aaatcaacta ctggtgcatc cggacggtca
     3961 caaacggcca aatccagcaa aacaccttca agtttcccgg aaatgactgc atcatgaatt
     4021 gccagcaaat tcgagccacg tcctgaagca aaaacggcaa ttctcatatt catttccaac
     4081 aaacctccgg tttattaccg gaaacaattt gaccgatccg gaaaacaggt tggccttctt
     4141 tagttaaaat ttcccgtgca attccctcat tagtttccgc aacacaaatc acgaaaccaa
     4201 ttcccatgtt aaataccgga aacagttcct catccggtaa tccactgact tgctggagca
     4261 aagaaaaaat cggcaattca ggccatgaat tgtaatgctc acttttttca atttctgcaa
     4321 caggcacaaa accttccggt gttttttcgg gaagcatccg gggtacattt ccaggcaaac
     4381 ctcctccggt aatgtgtgac attccgatga tttctatttc tgctaataat ttcaaaattg
     4441 gtttaacata aattaaggta ggagtcagca aagcttcacc taaagtttct gttgttcccg
     4501 gaaggatatc tttgaggttg agcttatgat ccttcagaat cttgcggacc agtgaaaacc
     4561 cattgctgtg gacgccactg gagggcaagc ccagcagaag atcaccaatt tctatccgct
     4621 taccgtcaat caattttttc cgctcaacag caccaactgc aaaaccggct aaatcgtatt
     4681 caccagagtc atacattccg ggcatttctg cagtttctcc gccaagtaaa gcagatcctg
     4741 attcaagaca accttcactg attccactga caatatcttc cacacgctcc ggaagcagtt
     4801 tcccacaagc caaataatcc agaaaaaaca gcggctccgc tccctgaacc aaaatatcgt
     4861 tgacacacat ggccaccaga tcaataccga tagtgtcatg acggtctaat tcaaaagcca
     4921 acttcagctt tgtacctact ccgtctgttc cggaaaccaa cacaggattt ttgtacttgc
     4981 tttggttcag ttcaaacaat ccgccaaatc ctccaagtcc actcaaaact tccggacgta
     5041 atgttctttt ggctaaaggc ttgagtttct caaccgcttc atccgcagca gctaaatcga
     5101 ctccggcttt ttgataagct ttcaagtttt tttaagttaa tgatttttaa atttggcaga
     5161 tttaacaagt cggtgtggga tagtttccat tgaaacaggc cagacaatga cctcgatctc
     5221 gcgttgaatc attcctgtca gtggcagaaa tcattccttc agcagaaata aaatgcaaag
     5281 aatccgcacc tattttttga catatttctt ccggagtttg ggttgctgaa atcagttcca
     5341 tacgccgtgc tgtatcgatt ccataaagac agggatgcgt aatcggtgga gatgtgacac
     5401 gcatatgaac ttcggtggcc ccagcagctt tgagtaattc cacaatccga ccactggttg
     5461 tgccgcgtac aattgaatca tcaaccaaaa ttactcgttt cccctcaact gcttttctta
     5521 caacacttag tttcatccgg acacctttac gtcgcagctc ctggtttggc tgaataaaag
     5581 tgcgccctac ataacggttt ttcatcatac ccatttcata aggcaaaccg ctttcctctg
     5641 caaatccaat cgccgccgcc agaccggaat caggaacccc aatcaccaca tcagcttccg
     5701 ttggaacttc gcggcaaatt tgacgcccca tttctttgcg cacattgtgc acgctgattc
     5761 cgtcgatttc actgtccgga cgggcgaagt aaatatattc aaaagaacaa atcgagcgtg
     5821 agcttggttg catgaatgtt tgtgaatgca atccctctgt gtcaatcacc agcatttcac
     5881 cgggttcaat ttcccgcaca aattctgctc cacatacatc cagcgcgcaa gtttcagaag
     5941 ccaccaccca accctcatcc agcttgccta gagcgagtgg atgaaaagct cgtggatcct
     6001 gtgcagctac cagttgagtt tcagtcataa aaagcagggc atacgcacct tcaatcacac
     6061 gcaacgcatc cggaatcgcc tgctcaatgg gtggtccgga acgtgcgatg agatgcggaa
     6121 caacttccgt atcactgttt gtctgataaa tagaaccttg cctttccaac tgatttcgca
     6181 agcgtttggc attaaccaga tttccattgt gcgccactgc gacttgtcca ccggtgtaac
     6241 gaaacacaag cggttgagcg ttcaccaaac cgctgtcacc ggctgtggtg tagcgcacat
     6301 gcccaattcc gatgtgccct gcaagctgga gatgttgatc ctgcccaaat gcttcagtca
     6361 ccagtcctgt accgcgatgc gtgtgaaatt tttctccatc ggaaacacat atccccgcgc
     6421 tttcctgacc acggtgctgc aacgaatgca atccgaaata ggtcaactct gctgcctgag
     6481 tatgattaaa aacgccgaag atgccgcaca tgaaaaagtt tgtttaagat gttaaataaa
     6541 taataatact ttcgtaaaaa ttcctaaaac aatgtcaatt ttgagcgaag cgagaaatct
     6601 tgcacaaact actacactaa tatcataaag attttttact ttttgcaagg ccgtaataaa
     6661 ttactatacc gaaatttcca tttcccgccc aatcgcattt ttccataaca ctcgtaatgg
     6721 attcaattca gcttgaatga ccggagtgcc gttcagtgaa atatcgaagc gtccagtact
     6781 gcccccacag ttcgaaccgg aagttccact ggaaaccacc ttaccgatac gggcgcatgg
     6841 tacacccttt ttctgcatga cgacttctac caactccgta ttatcaggag aaatactcag
     6901 caaaattcgg gattgagact cactgaataa agcaatatcg ggacgtaaat caatctccca
     6961 gtttactaat attccaaaat tttctgtaca tgaagtcaac aatgccaaag ccaaaccacc
     7021 ctgcgaaaga tcatgtgccg aattcacaat tccagaacga attatttcga gtaatgtttg
     7081 ctgtaatgtt cgctctttat ccagatccag ttccggcggc attcccgtga tttttcccag
     7141 ctgcatttgt tgataggaac ttcctcccaa ctcgtttttt gtatctccca gcaggagcaa
     7201 aacatctcct gaattttgaa aaaactgagt cgttgtgtgg tcattatgtt caatcaaacc
     7261 aaccacgccg accaccggag tcggataaat cgcctcaccc tcggtttcgt tgtagaggct
     7321 gacattccca ccggtaacag gcaatttcag aaagttacaa gtttcagcga ttccggaaac
     7381 ggcttgttcg agctgccaga atatttccgg tttttccgga tttccgaaat tcagtccgtc
     7441 agtaattgcc aagggatgag cacctgaaca aactacatta cgcgcggctt cagccaccgc
     7501 aattttacct ccggtttttg gatcaagaaa aacataacgt gagttgccgt caatactcat
     7561 ggcaagagct ttgtcggtac cttccacacg taccactccc gcatctgatc cggggcgcac
     7621 cgatgtgctg gtacgtacca taaaatcata ttgctgataa acccatgatt ttgaagccaa
     7681 ttccggagtt tgaatcagct gctgaaaaac gatttgcaga ttttccggaa cgccccaagt
     7741 tattgcggtt tgctgttgcc gctcaataac ttcttgcggc atttttgatt cacgctgata
     7801 aaccggacta gcgccgcaaa ctgtattcaa aggcaattct gcaacctttt ctccgtgatg
     7861 cttgagtcgc aaaacttgtt cttcaatcac atgtccgacg gtgacagccg gaacatccca
     7921 ctttttgaaa atcgcgaaaa cctcttcttc acgtcctgga ttggtcacaa acaacattct
     7981 ttcctgtgat tcagaaagca tgatttcata agcagacata ttttctgcac gctgcggtac
     8041 caaattcaaa ttcatctcca ttcccacgcc gcccttgtcc gacatttccg tgctggagca
     8101 agtcagcccc gcagctccca tatcctgcac gctgtatatc gcgccgcttt cgtaaacttc
     8161 caaacatgct tcaatcagca attttcccat gaacggatca ccaacctgta ccgccggacg
     8221 tttagcatca gattcttcat tgagttcttc cgacgcaaat gttgctccgt gaataccgtc
     8281 acgtccggtc gctgcgccta cataaattac tgaatttcca ataccttcag ctctaccgtg
     8341 ctgaagttga tcgtggcgta aaatgcctac acccattgca ttgactaatg ggttacctgt
     8401 atagcattca tcaaaacaaa cttcgcctcc tactgttgga acaccgatgc aattgccgta
     8461 tccggctatt cccgccacta catttccaaa tagatagcgt gtccggctga tactgttttt
     8521 gtctgcttca ctgtctgaaa gcggaccgaa acgcaatgaa ttgagtaccg ccaccggccg
     8581 ggcgcccatc gaaaatatat cccgtaaaat cccgccaact ccggttgccg caccctgata
     8641 cggctctatt gccgaagggt gattatggct ttcgactttg aaaaccactg catatccgtc
     8701 accaatatcg acgacccccg cgccttctcc cggtccctgc agcactttcg gaccatcaac
     8761 cggaaaacgt gaaagcagcg attttgaatt tttgtaggaa caatgttctg accacatcac
     8821 gccaaaaatt ccggtttcag tcaggttggg acgtcgcccc agcaattcga caacgagcga
     8881 gtactcgtca tcgttgagcc ccatttgttt gtatactttt ttgcagtgga tttgatccgg
     8941 ggtgggttcg tcagggtgca tcaggtgtat taaatttcag tatctatttt gatctcttgt
     9001 tttaacaaaa ctttggacac atacatcaca cattttctaa ctcagtacct tagttcctca
     9061 tagattcaaa tagaattcga ccatccgtac ttcccatcca atcagccatg gctcgttccg
     9121 gatgcggcat catgcccagg acattacccg cttcatttgt gatccctgca atattggcta
     9181 cggaaccgtt tggattgtca cctgaataac gaaaaacgat ctgcccgtta tcttccagtt
     9241 tttgcaaagt atcagaatcg caagtgtagt tgccttcccc gtgtgcaatc ccgaaacgta
     9301 catgagtgct agttcttcca gcgtatcctg tcgtgaagga agttgctgtg tttgagactt
     9361 ccaggttaac agtcttgcaa atgaatcgga gtccggaatt gcggtgcagt gcgcccggta
     9421 acagaccgca ttcagtaaga atttggaatc cgttgcaaat gccgagcaca cgggttccag
     9481 catctgcggc acgctgcaaa gccttcagaa tcggagcacg tgcagcaagt gctcctgctc
     9541 tcagagcatc gccataagag aatcctcccg gaaccaaaac tgcgtcaaaa ccatcaagtg
     9601 attcactggt gtaccacaca tattcagcct ggattccttc tatgtaattg gcggctttgt
     9661 aaagatcaaa atcacagttt gaacctggaa aaactataac cgcacaacgc atcaatcctc
     9721 cactatttca aagctgtaat cttcaatcac agaattgaca agcagtgcct cacacatttc
     9781 cttcagccgg gattcagctt tttgagaatc gctttcttcc agccagactt cgatcgattt
     9841 tccgatgcgt acattttgtg cttccgggaa cccaagtgtt tgtaatgaat gcaacacagc
     9901 ctgaccctgt gggtccagaa catcttgttt cagagaaaca gtaacaattc ctttaagcat
     9961 gtaagtggaa agtgaatgag ttttttagct ggaatttaag cgtcgtaaaa cctcagcata
    10021 ggcttcagtt acaccgccaa ggtcgcggcg aaaacggtct ttgtccagtt tttcacgagt
    10081 ttccgcatcc caaaatcgac aggtgtccgg agatatttca tcagccagca ataaattacc
    10141 tgaagaatcg cgcccgaatt ccagcttgaa atcaaccagt aataagcccc gaggtaacaa
    10201 taatttcagt aaacagtcat tgacctccaa ggctcgtttt cgcaattcat ttaattcttc
    10261 atctgttgct aatccgagcg tgtggatatg aacatcagcc aacagtggat cacccaattc
    10321 atcgtccttg taataaaatt cgactatcgg ttccggaagt tttcggcctt cttcccagcc
    10381 aaggcgctta ctcaatgaac ccgctgaaaa atttctagtc acaacttcca ggggaacaat
    10441 ttccacgcgc ttcaccaatt gttcagtgtc agaaattttt ctaacaaagt gactcgctat
    10501 tccctgctca gctagatatt tgaagaagaa agtagtaatt tcattattga gctgtccttt
    10561 gccttccagt ttttcaaatt tttcaccatt gaacgcagta gtatcgtttt tataggcaac
    10621 ccgtacaatg tttggatcat ctgaagaaaa tatttttttt gctttgcctt cgtaaagcag
    10681 agtcccggac atggttattt atgatttatt gtcagccgct aaaagcgcta aagattctga
    10741 aataaaatac atgttaccgc aattattgac agctaaatat tacagcattt tgtaaagcaa
    10801 agcctcatcc caaataccca gtttgaccag aggttccagt agggattccg gtgagtctgc
    10861 cgttaaattt aagtgtccca ttttgcggcc cggacgacat tcttgctttc cgtaaagatg
    10921 cagttttgct tctggaggca agtcttttaa tcttttcagc agcaagggta agtgctcgcc
    10981 caaaatattg agcattatgg caggtttata catttccgtg gagcccagcg gaaaatttgt
    11041 aatcgccttt aaatgctgtt caaactgaga agttgcgcaa tcgtttagag taaaatgtcc
    11101 tgaattgtgt gggcgaggag ccaactcatt caccatcagc tttccgtcag gcaaaacaaa
    11161 catttccaca gcaagtacac ctacaagttc caatccttct gcaatttgtt ttgctaataa
    11221 ttgtgcctgt tcgcgtactc tggaatctaa cctagcaggt attcggctga tggtcagtat
    11281 atttttttca tgaacatttt ctgcaaccgg aaaacaacta atttcaccca gacgattccg
    11341 tgcaacaatc actgatactt ctctttcaaa cgagacaaat tgttctgcaa caatttctgt
    11401 ttttgtacta tatagagtgg aaaatgcttc aggaatttgt tctactttag aaatacgaac
    11461 ttgccccttg ccatcgtatc caccggtaac ggttttcagc atcagttcgc caagcgattt
    11521 caatgccagt gaaagttcgt tttctgtagt gattttttgg aaaggagcaa cgggaatatt
    11581 aagtcgtaca agtgtttttt tttcacgtag acgattttga gcaatccgga gtatttcact
    11641 gccttgcgga acatccactc tttcctccag ccaggaaacc gcttctgcat ccacattttc
    11701 aaactcgtag gtaacaagtt gacatgaatc ggctaatttt gccagcgctt cagggtcagt
    11761 gtaacttgca gtaatttgtt cagctcctgc tagcgcagcc gggcagtcag gatttggttc
    11821 gagaaccata aaccgaaaac ccattgcatg accggcgagt accagcattt gtgccaactg
    11881 tcctccacca agaaggccaa tcgttgcgcc gggctgcaaa ggttccggat ctaaattcat
    11941 ggtaattctg cttccagcac ggttttacgg actcgttcac gacgccgaat caaacgcttg
    12001 cgtagttcag gatcattatt tgcgagaatt tgtgcggcca gcagtccggc attgtgcgca
    12061 cctgcacctc cgatagcaac cgtcccaacc ggaacaccac gcggcatttg cacgatcgac
    12121 aacagcgaat cctgtccgtt tagagtggaa gattttaccg gaacgccgat tacaggaagc
    12181 agagtttgcg cagcaaccat tcccggaaga tgtgctgcgc caccggctcc agcaataata
    12241 atttgcagtc cccgctcttc tgcctgtgtc gcataagcag acatccattc cggagtacgg
    12301 tgggccgaaa ctacacgttt ttcatagctg acgtttaatt cgtccagcac ttcgcaggct
    12361 tccctcattg tttcccaatc cgaagtactg cccatgataa cgccaaccaa aggataattg
    12421 acttttttct gtgccatttt attttatgtg aaaaatttta taaaccatag tgttctgcta
    12481 aacgcacaaa tcccggacga gaattttcga ttgtttcctg agtacaacaa agcgaaattc
    12541 gaaaatgtcc cggcccccca aaaccggttc caggtacaag taaaatgttt tcctgctgtg
    12601 cccgtttcac gaatgcaaca tcatcagaat caggtgattt aggaaaaatg taaaatgcac
    12661 cctgaggttt gactgcttca aagccgaggt ctgtaatcat agacattatt ttgtcacggg
    12721 atcgttcgta aacggagata tcaaccctcg cgtttccaag cagaggcaat attttttgca
    12781 tcatcgcagg tgcattgaca aaaccaagaa tacggttgca aagtacgagt gctgcacgca
    12841 actcttcacg ttcaataatg tccggatgaa tagcaatata acctattcgc tcaccgggaa
    12901 gtcccagatc tttggagtgt gaattaacta aaattgaatt cggatagctc agaaatgcgt
    12961 tgcagttagt taacccatca aataccagat agcggtaaat atcatctgag accaaataaa
    13021 tcggatgacc aaattccttc tctttgtgaa gcagcaactg ccctaattta tccaatgtct
    13081 cctgtgaata aactgcaccg gtgggattgt ttggactgtt gataattaca atttttatac
    13141 gttcacttaa gcttacttcg atggagttta gatccggttg aaattccgca gtagtttgtg
    13201 ctgttttcag gcagccgccg tgattttgag catatgcgcc gtattctaca aaaaacggtg
    13261 caagagccaa cacttcatca cccggctcaa gcagggcttt gaagaccaca ttcaggccgc
    13321 ctcctgctcc aacgcacatg accacatctt cactttcaaa aggatgaccg gtttcatcct
    13381 gcattacgcc agcaacgtat tcccgagttt ccgcaaatcc ggcatttggc atatagcggt
    13441 gcattcccgg ttccggattt tccaaaagtt gccttaattt gacatgtact tcttccggag
    13501 gttcaaggat ggggtttccc agggaaaaat caaacacatt ttctgaacca tgctctgctt
    13561 tgagacgtgc tcctgcttca aacatttgac gaatccacga gcctttctca atggattgcc
    13621 gaatcgaatt tgctattgac attaggtctc tacctgtttg caaaaacagt tttgaaaaat
    13681 tctggaagac gagtaatttt ggaattactg caataacagg agtgacagat tctgcaggac
    13741 actgtcaaga agtataatct caggtaaaga gatttttaat ggatttgaca aatttatcac
    13801 gaatcggatt gctttcgtcg tcacagcaat catcgaattc cttcaacaat tctatttgac
    13861 gttcagacag tttggtcgga gtttctacaa caatcctgac gtgcaaatca ccacgtccgc
    13921 cgcctctcag gtgtgctatt ccctggtttt tgagacgaaa tattttgtga gtttgagtac
    13981 ctgcaggaat tttaagcctt gcttttcctt ccaaagtagg aacttccagg tcagtaccaa
    14041 gtgttgcttg agcaaaacta attgggactt cgcaaaagat atctgcaccg tctctctcaa
    14101 aaatagagtg atcttttaca ctcaaaacta tgtaaagatc tccgctagat ccgccattta
    14161 caccatgctc accttcaccg gtaagcttaa tcctggatcc actggaaaca ccggcaggaa
    14221 tgtttaccct tatacgtttt gtttttgaaa cccgggtacg tccttgacag gttttacatg
    14281 gattggaaat ggttttgccg cgccccccac aactgctgca ggttgtggct acactaaaaa
    14341 acccctgttg gacccgttgt tgacctgtcc cgccgcaggt gccgcaaatt tttatatctt
    14401 tttcagattc tgcgccaaga ccactgcatg aatcacaggt ttccatgcgt ggaacatcaa
    14461 tttcttttga cgaaccaaat acggcatctt caaaagaaat ttccatatta tactgtaaat
    14521 ctgaaccacg ctcaccgcga cttgctgtgc tcccaggaga gccactgaaa aactcgctga
    14581 aaatatctcc gaaaacatcc ccaaatccgc tcccgccggc agcaccaaat cctcccattc
    14641 cactgcccat accttcagca gcatggccga attgatcata acgcgatctt ttttctttgt
    14701 ctccaagcac ctcaaatgcc tctgatgctt ctttgaattt ctcttcagat tttttatctc
    14761 ccggattacg atctggatga tgcttcattg cgaccttacg gtacgctttt tttagttcgt
    14821 ctgaagaagc attgcgggat acacccaaca cctcataata atcgcgtttt gccatttaaa
    14881 gttccaaata ccaagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccaa
    15001 gttccaagtt caaaatctca agttacaaaa ttcaagttcc aagctcatcg aacattgaca
    15061 ccatactgcc tgctacttgt cacttttttt tacttcctca aaatctgcat caactatgtc
    15121 gtcatcatct ttgcctgatt tttcatcatt tccgggtgca tcctcgcttc cttctccaga
    15181 accatctccc tgctgttctg cttgctgact gtataataat tcagccattt tgtgtgatgc
    15241 ctgagtcagg gcctcaagat cactctttat ggcttccata tcatctgttt ccatactctt
    15301 cttaagctgc tcgctacagg cttcaatttt agttttatct tcttccggca acttgtctcc
    15361 gagatcactt atagattttt ctgtactgta aatcaatgtt tccgcctggt tttttacttc
    15421 aacactttct cgttttgctt tatcttcatc tgcaaactgc tctgcatcat ttatcatttt
    15481 ttcgacatct tcttccgaga gacctcctga agattcaatt ttaatagcct gttctttccc
    15541 tgttcccaaa tctttagcac tgacgtgtac aattccgttt gcatcaatgt cgaaggtcac
    15601 ctcaatttga ggcatacccc ttggtgcagg aggaataccg accagctcaa aacgtcccag
    15661 ggtcttgtta tcagaagaca tttcccgctc gccctgcagg acatgaatac taaccgccgg
    15721 ctggttgtct gcggcagtag agaaagtctg actttttctg gtaggaatgg tggtgttacg
    15781 ttcaatcagt ttcgtcatta cactgccaag agtttcaatt ccaagcgaaa gaggagtaac
    15841 gtcgagaagc aatacatcct tcacatcacc ggtgagaaca ccaccctgga tagcagcgcc
    15901 gatggccaca acttcgtccg gatttacgcc tttgtgcggc tcttttccaa aaaattcttt
    15961 gaccatttcc tgtactttag gcattcgcgt catcccgcct accagaatta cttcattgat
    16021 atcctttgcg gtgcatcctg cgtcttttag tgctttacgg caaggttcaa gacagcgatt
    16081 aaccagatct tccgctagtg attcaaattt tgcccgtgtt attttaagat tcaggtgttt
    16141 tggaccagat gcatcggccg taatgaaggg caaattaaca tctgtttcag agctgcttga
    16201 caattcatgt ttggcttttt ctgcattttc tctgagtcgt tgaagagcca ttttatcttc
    16261 gcgcaaatcg attccgttat cttttttaaa ctcatctgca aaataactta tcagccggtt
    16321 atcaaagtca tcgccgccaa gaaatgtgtc gccattggta gctttcacat caaaaactcc
    16381 ttctccaatt tccaatatag aaatatcaaa agtaccgccg cctaaatcaa agactgctat
    16441 tttctcgtca tgttttttat ccaaaccata agccattgca gctgccgtag gctcattaat
    16501 gatacgcagc acttcgagcc cagctatttt tcctgcatcc ttggttgctt gacgttgggc
    16561 atcactgaaa tatgccggta cagtaataac agcttctttt actgattcac ccaaatattc
    16621 ttctgcagtc tgtttcattt tttgtaatat ggaagcagaa atttcctgag gagaataatt
    16681 tttgtcatcc actttgacat aagctaaatt ttgatttcct ttaacaactt cataagacac
    16741 catctttttc gtatgcttag cttctgctga tttatattcc tgccccacca agcgcttgat
    16801 ggaatagatt gttcgcaaag gatttgtaac agcttgtctt tttgccacct gtcctaccaa
    16861 acgttctcct tcgtcattga aagcaaccat cgacggtgta gtacgtgttc cttcagcatt
    16921 aggaatgacc ttcggttccc ctccctccat gatgctgaca catgaatttg tggttcctaa
    16981 atctatgcca atgactttac ccataatttt tctccttgtt ctttaattaa tacttcccct
    17041 ggtaaaactt actcattaat tacgagggga ttccgtttaa cttttactaa taaattccag
    17101 cttaataact cagttttttc ctgataccct gaccatggca gggcgaatga tgcgatcatg
    17161 caggaggtat cctcccaggc attcctccac aatatgattt tctggaacag aatctgactc
    17221 aacaacacca actgcctggt ggaaattggg atcaaagact tctccaattg tatcaactct
    17281 ggacacccca aatttttcaa aaacttcatg tgtcatttta ttgaccatct ccagtccctc
    17341 tatcatactg tcaacatcat tattttcact ttttgcatgg gagatagccc gttcaagatt
    17401 atccagagac ggtaacagtt ctttaataaa attgagatga aaatacttca agcgatcaga
    17461 gctttcgcga atcatacgct ttttatagtt ttcaaactct gcatttactc tcaggaagcg
    17521 gtcttccata ttctgtgcac gaatacgttc cttttccaaa agatcttctt cagcagatgc
    17581 agtttcctta ttatctgcgg aagtgccgga aatttctgca gcagtcactt cttcaatatt
    17641 tccattcgct ttttcttttt tgggtttatt tgttttctgc tttttcacgg cagtttcttt
    17701 caattttctt gcactcataa cgtctatgtt tcagattcag tcgtaagatc gttcggttat
    17761 agtttcagac aaaattttgg cagtatattc cactatagaa attatccttt tataatccat
    17821 acttgtgggg ccaacaactc cgatggaacc taaaacattt tgattgttcc tgtaactagt
    17881 catgattagt gaacaaccct gcaattgttc aaagtcgttt tcaatgccaa taaaaatatg
    17941 aacacctttt tgcctcattg aatcatcaag gattttcatt atttcgcttt tctgattaaa
    18001 agcttcaatt agagatcgta ctgaggactg cttatgaaaa tgtgaatcca gcaacaggtt
    18061 catctgtccg tctatcaaca tttcgcctgg attatttagt tcaaaagctt ttttacccag
    18121 acgaactgct tgagctaaaa gatcgtggta gcggtttcga tcatccacca ggtattggtg
    18181 tatcttttct ctaatttcct gcatagaatg atttttaaat ttttcattca ggaatgaggc
    18241 tactgagtta agaaaatctt gtgaatggct gtccttgaca aaaataatct tgtcacggac
    18301 cacacctgct tttgtgacta aaatcactaa gattctgtct tcactaattt taattaattc
    18361 aatccgttgc aaacgactga cagaagcctt gggtgtactc actacacagg caaatttggt
    18421 gagatctgcc aacaagtgaa ctgcctctgc caaaattcct tccacctgca ttccctgttc
    18481 agtggaaacc agagtaattt tttgtttaat ctgttcagga acactgcttg gtgtagacaa
    18541 ggaattgacg taaaatctgt aggctttatc ggtaggtaca cgtcctgcgg aggtatgggt
    18601 ctgaatcaga aagcccaatt ctgcaagatc cgacataatg ttgcgaatcg tagcgggtga
    18661 aagcgtgact ccaatcgatt ttgaaagtgt acgtgaaccg attggctctg aagattgaat
    18721 gtaattttct atgatgctgt taagcactaa ctgtgcacgt tcattcaaaa ccaagtcttc
    18781 cagtaatcct ctttttccgg taactgctgc ctttttcccc aaaagaaagt ctccatgcac
    18841 attgttaatt ttttagcact cattaggtgg tagtgctaat aatttaacat ttattagagg
    18901 cataagtcaa gcgaaagata aaacatgaca atattaagag aatatttgtc agtttagtca
    18961 ggaaggaaga aaaagcgaca ccagtgaata ttctgaaaga tgatatacca gcatccaaaa
    19021 agatttataa gtgatttctc aacttgcggg attccaggaa caagtctatg atgcctcgat
    19081 atttttgttc aagttcgtct tgttctctaa atgattgatt ttttactaat tttaatttag
    19141 agtcttgtga ctggaaggtg gaaatgtgtc ttagaatcca accttcctta ctcaaatcga
    19201 taaaaaacat ttttttggac tcaccttctt cttgatccgt gtaactgaaa cgtaccacta
    19261 ttcgtccccg ctgttcccct gtccagtagt gtataaaccc cagttccgga tgttccagat
    19321 ctccctcaac agcattgggc cagttttctt ttattttatc ttctgaaatc ttattcattt
    19381 tttttcatac aaaaagtaat tgctgagcac tcttacgagt ctccggatca gaaaatcgat
    19441 agactgcccg gtccataaaa taatgcataa atccgcttgc aaggccaatt cccactagaa
    19501 atctttccca agacgaattt tgcagccatt ccataaaaga agggaaaaat tgttcgctgt
    19561 agcgtcctcc agcagacaca gcttcgattt ccagaaacgg agtcataaga acagaaaaag
    19621 tacagaggag aaacaaaacc ttccagggtt tccaaaaaat atcaactgaa gagtctcctg
    19681 tgaaaccgat ttcgggattt gtttttaaag ttatatcatt tccagccatg tgaaccgtca
    19741 gccccaaagc gaccatccag tgctgaagtg tccacagcat aaaaaactga aatggttcca
    19801 actgatatgc gcccattacc attacaccta ttccaagtat atataaaatt cgtggaagtc
    19861 cagaatcatg tagaaacgca ttacgaatca tgaaaatagt gattcctgca acgagcaaag
    19921 ttccaagtcg caacaaaagt ggaattcctt caaaaaccca atctcctgtt agcaagggca
    19981 aaaaattctt ttcctgtaaa aacgacgtcc catgcaacag ttcggcaatc aaaaccaaac
    20041 cgcctccaac tccccaacaa taccacctat cccgcttatt ggacaatggt gcagatttgg
    20101 aattccagcg gtgatgatag agtcgtagga ggccgtagtg ctgtgccgca aagtgatgca
    20161 ttccccaagc gaagtccagt aaaatcatcc ctaatattct tacagaaacc gggaccggta
    20221 aaaccgactc tggaataaac agcacagcaa aaacaaccga tacaagcaga agcggaataa
    20281 acacaaaacg cccctgctga tttttgagaa gaggatggta agcctgtgtt ctccatgcca
    20341 aataaaatga actgaagcgg tgtgaaatcc agaaaaggaa tactcctgcg gcataaaaca
    20401 tttcctgtac aggcaagtca ctggtcgtga gcatcaggaa ggtcaaccat agtcctgagt
    20461 gcagccagaa tacatcccag gcaggtgacc tgacccagct attttcttta acacctactg
    20521 tcagacttgg gggaaattga caaatttttc tgagtaatga taatcccact gattcatgca
    20581 aaagaatgaa atttcaaact acagttatag taatgagtaa cacctaagta tctcatattt
    20641 accatgttgt ctcaagtgct gaactttgat gaatggaagc cctcaaaagg actcaaaag
//
LOCUS       DTVD01000205            5959 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4251_length_5959_cov_313.264566, whole genome
            shotgun sequence.
ACCESSION   DTVD01000205 DTVD01000000
VERSION     DTVD01000205.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_5959_cov_313.264566"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            2..760
                     /locus_tag="EYO68_07055"
     CDS             2..760
                     /locus_tag="EYO68_07055"
                     /inference="COORDINATES: protein motif:HMM:PF02353.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC07600.1"
                     /translation="MTPEFLDNQQYTELSILRYEAIYGHNYISPGGENTTDQFIDTLK
                     LLPEMRVLDIGCGLGGPAYRLASKYGVHVHGIDLSANMIRIAKTRLKEEGLQHLVTLE
                     QGNCLEIQTETTFNVVHSRDVFLHIKDKARLFEVIAKTLVPGGRLCFSDYCLGQAKSS
                     PEFKTYMRERNYSLHTVEDYTKLLEEAGFKNVCGEDRTDIFIQTLKHELKNLEKSSFL
                     KQEKNNLYKIWQKKIKRAERGEQGWGWFYGEKSQ"
     gene            757..1635
                     /locus_tag="EYO68_07060"
     CDS             757..1635
                     /locus_tag="EYO68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase"
                     /protein_id="HIC07601.1"
                     /translation="MTEILNKKVEALTFWQQPILCEPVKGGITNLNFRVEHGNEMFFV
                     RLGEDIPEHGVYRFNELAASRAAFACGISPEVVHAESGAMVLRFIEGKTLVEEDLRDF
                     SNLEKVLSLLKKCHREMPQHLPGSTLIFWVFQVIRGYAKTLREGKSRMIPQLQNFLNI
                     NADLEKTVGTIDLCFGHNDLLSGNFIDDGRRLWLIDWDYAGFNSPLFDLANLASNNEF
                     PEILEQELLEMYFEQTISADLWKRYFAMKCASLLREAMWSMVSEIHSTLDFDYVKYTT
                     DNLERFEKTFAKFEKL"
     gene            1632..4097
                     /locus_tag="EYO68_07065"
     CDS             1632..4097
                     /locus_tag="EYO68_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC07602.1"
                     /translation="MTNTLPKQAQIIIIGGGIIGCSVAYHLAKEGAKDVLLLERGQLT
                     CGSTFHAAGLVGQLRSSANITRVLKNSVELYRTIEGETGQATGWKMNGGLRLACTKER
                     WTELKRQATTAHSFGLEMELLSPNEAQELWPLMEVEDVIGAAFLPTDGQANPSDITQA
                     LAKGARMCGVTIVEETSVTGIRLVNQQVAAVQTENGEILCETVVNCAGQWANELGKMT
                     GVNVPLVSVEHQYLITEAIQGVTADLPTLRDPDKLTYWKEEVGGLVMGGYEPNPIAWD
                     SKPIPKDFIFQLLPENLEHFEQLMLPAVERVPALENAGIRKLMNGPESFTPDGNFILG
                     EAPEVKNYFVGAGFNAFGIAAAGGAGKALAEWILAGEPPMDLWVVDIRRFSNLHKNED
                     WVRNRTLELYGKHYTLSWPHEEHESGRPVLTSPIYEILKEQGACFGSKLGWERPNWFA
                     PEDETAQDIYSYCRQNWFPHVGEEHRAVRERVALFDQSSFAKFRIIGTDAEKALNRIC
                     ANNVAKPSGALTYTQMLNSKGGIECDLTVARLAKDEFYLVSGTGFRTHDSAWIRSQFL
                     PDEKVELHDITEEWATFSLMGPLAREVLAQVTENDLENENFPFGTCRNIEIKEELAPD
                     VPAVLALRVTYVGELGWELHLPRDSAESVYDVLMEAGKNSGIANAGYRAIESLRLEKS
                     YRTWGADITADTTPFEAGLGWAVKLNSGTHFIGREALLAQQNKPLQKRLACFTINDPD
                     VVLLGRETIYRNGEVVGWLTSGGWGYTVNKNIGYGYVRNPEGVDNEYFISGTYELEVA
                     TVRHSCKLQLGPLYDPKLERVRK"
     gene            4094..5008
                     /locus_tag="EYO68_07070"
     CDS             4094..5008
                     /locus_tag="EYO68_07070"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC07603.1"
                     /translation="MIPRGYLYVFGCASSFGLITTLAKLTYDEGASPQTVVFFRILAG
                     SLLMGVWSAYSHRNNLKYVIRKSLQATNLPIVFLIIAIGFCIAGMSLGYLGSVKYIPV
                     SLSVLLFFTFPFWVLLLNFIIHGIVVSWLKLFAFVLAFSGLALSLGPTWEVLDWRGIA
                     LVLGGSICSAGMIVGATKATQIISMSDLVFLSNTIGVILVGLILFFTDSFSLTQTTWV
                     WSGIATICILFVFGQLSLFAATKTIGSAQTSLMLNIEPLVSILAAVILLGESLVLSQT
                     LGVAVILVALLLASATQQGFSFFKKKNR"
     gene            5039..5821
                     /locus_tag="EYO68_07075"
     CDS             5039..5821
                     /locus_tag="EYO68_07075"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC07604.1"
                     /translation="MPKLTKRRVSEAQDLEESYRLADIVKQRQKTLAALKLKAGELVL
                     DIGCGVGFLTHEMALQVGKSGKVIGLDKNPEMISLARKRCESLQQTGFYKGDAGKLPV
                     DDQTLDAVSCTQVLLYVKDVPNVLAEMRRILKPGGRLVIVETDWRGVVLNNADDSLTN
                     KIFSAWDNSVPSPNLPVHLKPLLQKHGFSKIKVDPIPILNTEYSPSNFSHSMLKWISK
                     NAQNQGMINKEQRSNWLEDLQNRGQSDSFFFCVNRFLFTAFL"
BASE COUNT         1650 a         1185 c         1462 g         1662 t
ORIGIN      
        1 aatgacccca gaatttctcg ataatcagca atacacagaa ctttccattc tgcgttatga
       61 ggccatttac ggacacaact acatcagtcc cggaggagaa aataccaccg atcaattcat
      121 cgacacactg aaacttcttc cggaaatgcg ggtgctggac atcggttgcg gtttgggagg
      181 tccggcctac cgtttggcca gcaaatacgg agtccatgtt cacggcatcg atctctctgc
      241 aaacatgatt cgcattgcca agacccgcct gaaggaagaa ggattgcaac atctggtgac
      301 attggaacag ggaaattgcc tggaaataca aacggaaacg acctttaatg tggttcacag
      361 tcgtgatgtt tttttacaca tcaaagacaa agcacgtttg tttgaagtaa tcgctaagac
      421 tttggtacca ggtggacgct tatgcttctc ggactattgc cttggccagg caaaatctag
      481 tccggaattt aaaacatata tgagggagcg aaattattca ttgcatacgg ttgaagatta
      541 cacaaaatta ctggaagaag caggatttaa aaatgtttgc ggagaagacc gaacagacat
      601 atttatacag actctcaaac atgaattgaa aaatttggaa aagtcgtcct ttttaaaaca
      661 agagaaaaac aacttgtaca aaatctggca gaaaaaaatc aaacgtgctg aacgtggaga
      721 acaaggttgg ggctggtttt acggagaaaa gtctcaatga cagaaatttt aaacaaaaaa
      781 gttgaagcac tcactttttg gcaacagccg attctgtgcg agccggtgaa aggaggaatc
      841 accaatctta attttcgggt ggagcatggg aatgaaatgt tttttgtgcg tttgggagaa
      901 gatattccgg agcatggcgt ttatcgtttt aatgaattgg cggcgagtcg ggctgcgttt
      961 gcttgcggga tttctccgga agttgttcat gcagaatccg gagcaatggt tttgcgtttt
     1021 atcgaaggta aaactttggt cgaggaagat ttgcgggatt tttctaactt ggaaaaagtc
     1081 ctttctttac tgaaaaaatg ccatcgggaa atgccccaac atcttccggg aagcactttg
     1141 attttttggg ttttccaagt gatacggggt tacgcaaaaa ctttgcggga aggaaaaagt
     1201 agaatgattc cgcaactgca aaacttttta aacatcaatg cagatttgga aaaaacggtc
     1261 ggaacgattg atttgtgttt cggacacaat gatttgcttt ccggaaattt tattgacgat
     1321 ggccggagat tgtggctaat tgattgggat tatgccggat tcaacagccc cttatttgac
     1381 ctcgcaaacc ttgcctcgaa caacgaattt ccggaaatat tggaacaaga attgttggaa
     1441 atgtattttg aacaaacgat ttctgcagat ttgtggaaac gctattttgc gatgaaatgc
     1501 gcttccctgt tgcgtgaagc aatgtggagc atggtttctg aaattcattc cacacttgat
     1561 tttgattacg taaaatatac caccgataat ttggaacgct ttgaaaaaac ttttgccaaa
     1621 tttgaaaaat tatgacaaac actttaccaa aacaagcaca aattattatc atcggcggcg
     1681 gcatcatcgg ttgcagtgtc gcttatcatt tggcgaaaga aggtgcgaaa gatgtgcttt
     1741 tgctggagcg cggacaactg acttgcggca gtacttttca tgcggctggc ttggtcgggc
     1801 agttgcgcag ttctgccaac atcacccgtg ttttgaaaaa tagcgtggaa ctgtaccgga
     1861 caatcgaggg cgaaaccgga caagccaccg gctggaaaat gaacggtggt ttgcgtttgg
     1921 cctgcaccaa agaacgctgg acggaactca aacgacaagc caccactgcg cacagttttg
     1981 gtttggaaat ggagttgctc agtcccaatg aagcacaaga attgtggccg ttgatggaag
     2041 ttgaagacgt aatcggagcg gcatttttgc caacggacgg acaggccaat ccttcggaca
     2101 tcacgcaggc attggccaaa ggcgcacgga tgtgcggtgt caccattgtt gaagaaactt
     2161 cggtaacggg tatccggctt gtaaatcaac aagtcgccgc tgtgcaaacc gaaaatggag
     2221 aaattctttg tgaaaccgtg gtcaattgtg ccggacaatg ggccaatgaa ctgggcaaaa
     2281 tgaccggcgt gaacgtgcct ttggtttcgg tggaacatca gtatttaatc accgaagcga
     2341 ttcagggcgt gactgcggat ttgcctactt tgcgtgatcc ggataagttg acttattgga
     2401 aggaagaagt gggcggtttg gtgatgggcg gttacgaacc gaatccgatt gcgtgggact
     2461 caaaacccat tccaaaagat tttatatttc aattgcttcc ggaaaattta gagcattttg
     2521 agcaattgat gcttccggca gttgagcgtg ttcctgcttt ggaaaatgcc ggaattcgga
     2581 aactgatgaa cggcccggaa tcctttactc cggacggcaa ttttatttta ggcgaagctc
     2641 cggaagttaa aaattatttt gttggagccg gattcaatgc tttcggaatc gccgccgcgg
     2701 gaggtgccgg aaaagctttg gcggagtgga ttttggccgg agaaccaccg atggatttgt
     2761 gggttgtgga catccgccgt ttcagcaatc tccacaaaaa cgaagactgg gtgcgcaacc
     2821 gaactttgga actttacggc aaacattaca ccctcagttg gccgcacgaa gaacacgaaa
     2881 gcggtcgccc tgttttgacc tcaccgattt atgaaatttt gaaagaacaa ggagcgtgtt
     2941 ttggttccaa actcggttgg gaacgcccca actggtttgc tccggaagac gagacagcgc
     3001 aggatattta ttcttactgc aggcaaaatt ggtttccgca cgtcggcgaa gaacatcgtg
     3061 cggtgcggga acgtgtggca ctttttgacc aaagttcgtt tgcaaaattc cgtatcatcg
     3121 gcactgatgc agaaaaagca ctgaaccgga tttgtgccaa caatgtcgcc aaaccttccg
     3181 gtgctttgac gtacacgcaa atgctcaatt ccaaaggcgg gatcgagtgc gatttgaccg
     3241 tggcacgttt ggcaaaagat gaattttatt tggttagcgg aaccggattt cggacacacg
     3301 attctgcttg gattcgcagt caattccttc cggatgaaaa agttgaactg catgacatca
     3361 ccgaagaatg ggcgaccttt tcattgatgg ggccgttggc gcgtgaggtt ttggcgcaag
     3421 ttacggaaaa cgatttggaa aatgaaaatt ttccctttgg aacgtgccga aacattgaaa
     3481 ttaaagaaga actcgcaccg gacgttccgg cggttttggc tttgcgagta acttatgtag
     3541 gagaattggg ctgggaactg catttgcctc gtgattccgc agaatcggtt tacgacgttt
     3601 tgatggaagc cggaaagaat tccggaatcg ccaatgcggg atatcgggcg atagaatctt
     3661 tgcgtctgga aaaaagctat cgtacctggg gagcggacat aaccgctgac actacaccct
     3721 ttgaagccgg attgggttgg gcggttaaat tgaattccgg aacacatttt attggacgcg
     3781 aggctttgtt agcccaacaa aataaacctt tgcaaaaacg cttggcctgc ttcacgatta
     3841 atgatccgga tgttgttttg ctggggcgtg aaacaattta ccgcaacggc gaggtcgtcg
     3901 gttggttgac cagtggaggc tggggttaca ccgtaaacaa aaatattggc tacggatatg
     3961 tcagaaatcc ggaaggcgtg gacaatgaat actttatttc gggaacttac gaactcgaag
     4021 tcgccaccgt ccgtcattca tgtaaactac aacttggccc gctctacgat ccgaaattgg
     4081 aaagggtgcg gaaatgattc cacgaggtta tctatatgtt ttcggctgtg cctcttcttt
     4141 tgggctgata actacccttg ccaaactaac gtacgacgaa ggtgcttcgc cacaaacagt
     4201 tgtttttttc cgcatccttg caggaagcct gctgatggga gtctggtctg catactctca
     4261 caggaataat ctaaaatatg tgattcgtaa atctttgcag gcaactaatt tacctattgt
     4321 ttttttgatt attgcgatag gtttttgcat tgcaggaatg agtttgggat atttaggctc
     4381 agtaaaatac attcccgtca gcctcagtgt tttgctgttc ttcacctttc cattttgggt
     4441 tttgctttta aatttcatca ttcacggtat agtcgtaagt tggctaaaac tctttgcgtt
     4501 tgttctcgct ttttcaggtc tggctctgag ccttggacca acttgggaag tacttgactg
     4561 gcgtggaata gcacttgttc tgggaggttc aatttgttca gcgggaatga ttgtcggcgc
     4621 cactaaagca actcaaataa tatccatgtc tgatctggta ttcttgtcta acacaatagg
     4681 agtcatcttg gttggattga ttctattttt cactgactct ttttcattga ctcagactac
     4741 atgggtatgg tcaggaatag caacaatttg tattttgttt gtctttggac agttgagttt
     4801 atttgctgct acaaaaacca tcggttccgc gcaaacgagt ctaatgctga atattgaacc
     4861 gctggtttcc attttagctg ctgtcatttt acttggagaa agtttagtgc tgtctcagac
     4921 cttgggtgtt gccgttattt tggtagcact tttactggca agtgctactc agcaaggatt
     4981 tagtttcttc aaaaagaaaa acagatgatc tgcatttaca tattttcctt aattgatcat
     5041 gccaaaatta actaaacgca gagtttcaga agcccaggat ttagaggaaa gctaccgact
     5101 tgccgacata gtgaagcaac ggcagaaaac tttggctgca ctgaaactta aagctggtga
     5161 gctggtttta gacataggtt gtggtgttgg attcctcaca catgaaatgg cactgcaggt
     5221 tggaaaatca ggtaaagtaa ttggtttaga taaaaatcct gaaatgatca gcctcgcccg
     5281 aaagcgttgc gaaagcttgc agcagacagg attttacaaa ggagatgctg gcaagttgcc
     5341 cgttgatgat caaactttag atgcagtttc ctgtacacag gttttgctct atgtaaagga
     5401 tgttccaaat gtgcttgctg aaatgcggcg cattctaaaa ccgggagggc ggcttgttat
     5461 tgtggaaacc gattggcggg gagtggtttt aaacaatgcc gatgattctt tgacaaataa
     5521 aatcttctca gcctgggata attctgttcc aagtccaaat cttccggtgc atttaaaacc
     5581 acttctgcaa aaacatggat tttcgaaaat caaagttgac cccattccta ttctaaatac
     5641 agaatattct ccaagtaatt tttcacacag catgctgaag tggatctcaa aaaatgccca
     5701 aaaccaaggt atgataaaca aagaacaaag atcaaactgg ctggaagacc tgcagaatcg
     5761 gggacagtca gatagttttt tcttttgtgt gaaccgtttc ctctttactg catttttata
     5821 gataagaagt aaaaagttga ctggacagta acgtgttttg tcgtgtcact ccgaacaaat
     5881 gtgagttatt ttggcggttt cgttgtacgg atttgcagag ccgtctctac attcagtttc
     5941 acttcaaatc agggttcaa
//
LOCUS       DTVD01000206            5847 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4392_length_5847_cov_436.989296, whole genome
            shotgun sequence.
ACCESSION   DTVD01000206 DTVD01000000
VERSION     DTVD01000206.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4392_length_5847_cov_436.989296"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="EYO68_07080"
     CDS             complement(<1..910)
                     /locus_tag="EYO68_07080"
                     /inference="COORDINATES: protein motif:HMM:PF02615.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIC07605.1"
                     /translation="MLIQAEPLNNLVSTLLKKGGSCIEEAQVVADHLVRSNLAGHDSH
                     GVGMLPLYVRMLKEDFLRPNQKPELVNADGSILMFDGQRGYGQAVGKMAMVQAIEKCR
                     ESGLVLMTLRNTHHLGRIGTYGEQSIASGLVSLHFVNVTDHQPLVAPFRGSDARFSTN
                     PICLAMPGTEKQPPVLLDMATSRIALGKARVALNKNEALKDGLVIDHKGQPSWDPGVM
                     AGYLFPERPDNPPLGALTPLGEYKGYGLALFCELLGGLLSGGGTIQPEHQRQNSIINN
                     MFTLVIDPARLVDVPWMQHEVEAIVAH"
     gene            complement(1141..2331)
                     /locus_tag="EYO68_07085"
     CDS             complement(1141..2331)
                     /locus_tag="EYO68_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC07606.1"
                     /translation="MTKSNTRTAFDWADPMLFNEQLTEEERLIRDTARDFSQDKLMPR
                     VLEANRNEVFDREIMNEFGEFGFLGSTIPEEYGGVGASYVSYGLIAREVERVDSGYRS
                     AMSVQSSLVMHPIYAYGSEEQRTKYLPKLASAEWIGCFGLTEPNHGSDPGSMEARAKK
                     VDGGFKLTGNKMWITNSPIADVFVVWAKNDKGIIRGFILEKGMEGLSTPKIVGKMSLR
                     TSVTGEIVMDEVFVPDENELPNVSGLKGPFGCLDRARFGIAWGTLGAAEFCWMQARQY
                     TLDRIQFGRPLAANQLIQKKLADMQTEITLGLQGVLQMGRLMDLGKCAPELISLMKRN
                     NCGKALEIARLARDMHGGNGISDEFHVIRHVMNLETVNTYEGTHDVHALILGRAQTGI
                     QAFC"
     gene            2522..4387
                     /locus_tag="EYO68_07090"
     CDS             2522..4387
                     /locus_tag="EYO68_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07607.1"
                     /translation="MKQRRKNTSGTHRVPPDLDFRERLGALRNLPEFFRLIWSCNRLL
                     AVFNILLRVIRASLPLTMLYVGKLIIDEIVLISVTAGGSAVENPDMSILTTFVLIELG
                     LAVFSDLLGRGIALVDSLLGDLVSHEISLRLIHQSARLDLESFEDSEFYDKLERARRQ
                     ASSRILLMSQALTQLQDSITVFFLAAALITFNPWLLLLLAITLIPAFLGETHFNSQSY
                     SLMYGWTQERRELDYLRFAGASDETAKEVKIFGLSDFFGKRFQKLAGEYYQVNRNLSV
                     RRAAWGGLLSMLGSLGYYTAYAVIIYRTVYGELSLGDLTFLSGSFLRLRSLMEAILIR
                     FSSIADSALYLRDLFDFLEMEPRIRSKDESLPFPETIHDGFTFENVSFRYPQMEKWVL
                     RDVSFTLHPGEKLALVGENGAGKTTLVKLLTRLYDPAEGRILLDGHDLRDYNLDGLRD
                     AVGVIFQDYVKYHLTASENIAVGRIDDRNNANRIKEAAQRSLADKVIDKLPEGYQQMI
                     GRWFKQGTNLSGGEWQKIAIARAYMRDAQLLILDEPTAALDARAEHEVFHRFVELTFD
                     KCAVLISHRFSTVRMADRIVVLHEGKLLEHGTHEELLDAGGQYSELFQMQASGYR"
     gene            complement(4418..5494)
                     /locus_tag="EYO68_07095"
     CDS             complement(4418..5494)
                     /locus_tag="EYO68_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07608.1"
                     /translation="MRRIIMMFMQFESISRQIFNRGTVSLPTQTDLEGLADRVVESRW
                     YREALNRFSSNNAYGFSEERMLRVLMSIHTAAHFFEVPYPTLFCLFFQESKFDFLANS
                     ATGAKGIGQLTSIGLREVQRLRNASEMELKLQKTAFHLNRVYTDPQIQKWLENLGFKI
                     NFAKISPIPEKIEFTRLSSSFMREVGKELVKEGQSYGENTSLLWFLSKRLRRGDILSN
                     RFAHMHKVFSQMLEEQYASSQASAYNIETNILLSTILFSHYYRYRWRNNKQVFNLPPE
                     ARVILATSAYNHGQTGMRRFLINLKQEFPMLDFQALSSKKLRILFTIRRLSNAIKQSP
                     RKIKEVSRHVRNIMDCAEKRPLTS"
BASE COUNT         1583 a         1225 c         1270 g         1769 t
ORIGIN      
        1 cgtgtgcgac tattgcttct acttcgtgtt gcatccaggg aacatccact aatcttgctg
       61 ggtcaattac cagggtgaac atgttgttga taatgctgtt ttggcgctga tgttcgggtt
      121 ggatagtgcc gccgccgctg agtagtccgc cgagtaattc acaaaacagg gcaagtccgt
      181 agcctttgta ttctccgagc ggtgtcagag cacctagtgg aggattgtcc ggacgctctg
      241 gaaataagta tccggccatc actccgggat cccagcttgg ttgtcctttg tggtcgataa
      301 caagtccatc tttgagtgct tcgtttttat ttaaagcaac ccgtgctttt ccgagtgcta
      361 tgcggctggt tgccatgtcc agtaacaccg gaggttgttt ttcagttccg ggcatggcga
      421 ggcaaatcgg atttgtcgaa aaacgggcgt cagagcctcg aaaaggcgca accaggggtt
      481 gatggtcagt cacattgaca aaatgcagcg aaaccaatcc tgatgcaatg ctttgctcac
      541 cgtaggtccc gattcgtccg agatggtgag tgttcctgag tgtcattaaa accagtccgg
      601 attcacggca tttttcaatc gcctgtacca tggccatttt accgactgcc tgtccataac
      661 cacgctgacc gtcaaacatc aaaatcgagc cgtctgcgtt aaccaattca ggtttttggt
      721 tcggacgcag gaaatcctct ttgagcatcc gtacataaag aggcagcatt ccgacaccgt
      781 gactgtcgtg tccagccaaa ttggatcgaa ccagatggtc ggccaccact tgagcctctt
      841 cgatgcaact gccgcctttt ttgaggaggg ttgagactaa attattaagt ggttcagctt
      901 gaattagcat ctggtaactg aatgtcaggt gatttcatga gtctgttgtg acgtacttgt
      961 aaaaagttgt cattcccgcg taaaagagaa tccaggaatt ttgtatagct gaaatacttt
     1021 acctagatct gctcatggca tgaattcctg ctttcgctgg aatgaaaaat aaatttgaaa
     1081 aggttatcta aaaccataat gttagaataa tttgcaactg tcggttttcg atcaaatatt
     1141 ttaacaaaac gcttggattc cggtttgtgc ccttccaaga ataagcgcat gtacgtcatg
     1201 tgttccttca taagtattga cggtttccaa attcatcacg tggcggatga cgtgaaattc
     1261 atcggaaatg ccattaccgc cgtgcatatc acgggcgagc cgtgcaattt ccaaagcctt
     1321 tccgcaattg ttgcgtttca tcaatgagat caactccgga gcgcatttcc caaggtccat
     1381 caagcgtccc atttgcaaaa ctccctgcag acccagcgtg atttcagttt gcatgtcagc
     1441 cagctttttt tgtattaact ggtttgccgc caacggacgt ccaaactgaa tccggtccag
     1501 tgtatattgc cgtgcctgca tccagcaaaa ctcggccgca ccaagcgttc cccaggcaat
     1561 gccgaaacgt gcgcgatcca gacagccgaa cggacctttc agtccggaca cattgggcaa
     1621 ttcattttcg tctggaacaa agacctcatc cataacgatt tcaccagtaa cagatgtccg
     1681 cagtgacatt tttcccacaa tttttggtgt actcagacct tccatgccct tttccaaaat
     1741 gaagccgcgg attatgcctt tatcgttttt agcccaaacc acaaagacat cagcaattgg
     1801 tgaatttgta atccacattt tgtttccagt cagtttaaat ccaccatcaa ctttcttcgc
     1861 acgtgcttcc atactgcctg gatcggaacc gtgattaggt tcggtcaaac caaaacagcc
     1921 tatccattcg gcactggcga gtttcggcag gtattttgtt cgctgttcct cactgccgta
     1981 tgcataaatc gggtgcatca ccaaactgga ttgcacgctc atagccgagc ggtaacccga
     2041 atctacccgc tccacttcac gggcaattaa accatatgaa acataacttg ctccaactcc
     2101 tccatattct tccggaatgg tcgaacccaa aaatccgaat tctccaaact cattcatgat
     2161 ttcacggtca aaaacctcat tgcggtttgc ttccaaaact ctgggcatta atttgtcctg
     2221 gctaaaatcg cgggctgtgt cccggatcag gcgctcttct tcagtaagtt gttcattaaa
     2281 caacatagga tcagcccaat caaaggccgt tctggtattt gattttgtca tcggattacc
     2341 tgaaaataat ttaaaactaa atagccataa tataatgaat aaatgggtaa aattgcaatt
     2401 tcaatctgaa atatttaatt gcttatgcta gcatctttgt aagaaattca agtaaatatc
     2461 tttacttaat caataaaact tactggacag acagtttttt cttaaactct gaataacatc
     2521 aatgaaacaa agacgcaaaa acacatctgg tactcataga gttcctcctg atttagattt
     2581 tcgtgaacgt cttggtgcac tcagaaatct gcctgaattt ttcagactta tctggagttg
     2641 caaccgtttg ctggcagtct ttaatatttt actgcgtgtc atccgggctt ctttgccgct
     2701 aacgatgctt tatgttggca aactcattat tgatgagatt gtattgattt cagttactgc
     2761 tggaggatct gcagttgaaa acccggatat gtcaatttta acgacatttg ttttaataga
     2821 gcttggtctg gcagtatttt ctgatttgct gggccgcggc attgctttgg tggacagttt
     2881 acttggtgat ttggtttcgc atgaaatatc tttgcgcctg attcatcaat cagcaaggct
     2941 tgatcttgaa agttttgaag attcagaatt ttatgacaag cttgaacgtg ccagacgtca
     3001 ggcttcaagc agaatcttat tgatgtcgca ggccttgaca cagcttcagg acagcatcac
     3061 cgtatttttt ttagcagcag ctttaatcac attcaacccc tggcttcttc tactgctggc
     3121 catcacgcta atacccgcat ttttaggtga aacacatttc aacagtcaaa gctattcatt
     3181 gatgtacggc tggacacagg agcgccgtga acttgattat ttgcgttttg caggagccag
     3241 tgacgaaacg gctaaagagg taaaaatatt tggtttgtct gatttttttg gcaaacgttt
     3301 ccagaaatta gccggagaat attaccaggt aaacaggaat ctttctgtac ggcgtgcagc
     3361 ctggggagga ttgctttcga tgctcggtag tttaggctac tacacagcct atgcagtgat
     3421 tatttacaga acagtatacg gagaactgtc actgggtgat ctgacatttc tttccggttc
     3481 ttttttacga ttacgttctt tgatggaagc aattctaatc cgcttttcca gtatcgccga
     3541 cagtgctctt tatctgcgtg acctgtttga tttccttgaa atggaaccgc gaatcaggtc
     3601 caaggacgaa tcactgcctt tcccggagac tattcatgat ggttttactt ttgagaatgt
     3661 tagttttcgt tatccacaga tggaaaaatg ggttttgcgt gatgtttctt tcactttgca
     3721 tcctggtgaa aaattggctc tggttggcga aaatggagca ggtaaaacca cgttagtaaa
     3781 actgctgacg cgcctctatg atccagccga gggaaggatt ttgctggatg gtcacgattt
     3841 gcgtgattat aacctggatg gattgaggga tgccgttgga gttatttttc aggattatgt
     3901 gaagtatcac ttgactgcat cagaaaacat tgcggttggt cgaattgatg atagaaacaa
     3961 cgcaaatcgt attaaggaag cggcccagcg cagtttggct gataaagtga ttgacaagct
     4021 gcccgaaggt tatcaacaga tgataggacg ctggtttaaa cagggtacca atctatccgg
     4081 aggagaatgg caaaaaattg caatagcacg tgcctatatg cgtgacgcac aactcctgat
     4141 tttggatgaa cctaccgccg ctttggacgc acgagcagaa catgaagttt ttcatcgttt
     4201 tgtagaattg acatttgata agtgtgctgt actgatttct catcgattct ctactgttcg
     4261 aatggccgat cgaatagttg ttttgcatga aggaaagttg ttggagcatg gaacgcatga
     4321 agaattatta gatgccggag gacagtattc tgagttattt caaatgcaag cctctggata
     4381 tcgttaacaa tgttacggta tacatattat tataccatca ggaggttaat ggacgttttt
     4441 ctgcacaatc cataatattt cggacgtgcc ttgaaacttc cttgatcttg cgaggggact
     4501 gcttaatagc atttgataat cggcgaatag taaacaggat tcttaatttt ttagaagaaa
     4561 gtgcttgaaa atccagcatt gggaactctt gtttaagatt gatcaaaaaa cgacgcattc
     4621 ccgtttgtcc atgattatat gcagatgtgg ccaatataac ccttgcttct gggggtagat
     4681 tgaaaacctg cttgttattg cgccatcgat agcggtaata atggctaaag agaattgtgg
     4741 acaaaagaat atttgtttca atgttatagg cacttgcttg tgaacttgca tattgctctt
     4801 ctagcatctg agaaaagact ttgtgcatgt gcgcaaaacg gttggagaga atatcccccc
     4861 ttctcagcct tttgctgagg aaccacagta agctggtatt ttctccatag gactggccct
     4921 cctttacaag ttcttttcca acttcccgca taaaagaaga ggaaagtcgt gtaaattcta
     4981 ttttttctgg tataggagaa attttagcaa aatttatttt gaaaccgagg ttttctagcc
     5041 atttttgtat ttgcgggtcc gtataaactc gatttagatg aaatgcagtt ttctgcagtt
     5101 ttaactccat ttctgaagca ttccttaacc gttgtacttc ccgtaaccca atactggtga
     5161 gttgtccaat tccttttgca ccagttgcgc tgttggccag aaaatcaaat tttgattcct
     5221 gaaaaaacaa acagaataga gtagggtatg gtacttcaaa aaaatgtgca gccgtgtgaa
     5281 tggacattaa aactcggagc atacgttcct cagaaaaacc gtaagcatta tttgaagaaa
     5341 atcgatttaa tgcctcacga taccagcgac tttcaacgac gcggtcagcc aaaccttcta
     5401 aatctgtttg ggtcggtaaa cttactgtgc ccctattgaa tatttgccta ctgattgatt
     5461 cgaattgcat aaacatcatg ataattcttc gcattctttc gttgaaaaat tgattcttct
     5521 ttttttcaaa ataagatcga tgcagattcc caacggactg tagcagttgg tttgtgctta
     5581 aatccggaat ggattgaaga atttctaaat gttttactga atttgcagga gaatcgggga
     5641 ctttaagttt gactttagta gttggaaaaa tcctcggatg atactgtgaa tgctgaggat
     5701 aataggctat attcggggat tggctagttt cagggacgat caggaaaagg gagagaagaa
     5761 ttataaataa aacaggcaat gataaaacat gttgcctaaa tgaagtcaga aatatcaaaa
     5821 tttttttact aaactccatt cttcttt
//
LOCUS       DTVD01000207            5808 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4451_length_5808_cov_371.047801, whole genome
            shotgun sequence.
ACCESSION   DTVD01000207 DTVD01000000
VERSION     DTVD01000207.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4451_length_5808_cov_371.047801"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            208..1566
                     /locus_tag="EYO68_07100"
     CDS             208..1566
                     /locus_tag="EYO68_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIC07609.1"
                     /translation="MLEKYKSITKEIIDLAKQYGAEQASVSLANSTSFQVDVREEKIE
                     SLQESGSSGVHLTVSKNKRRSTVSSNDLRIETLGPLIRTTMDALPFMGEDEFYSLPDP
                     ALQGRAPGNLEFLDPDFNKLSSEEKVQIPLDLEQLTLNSDRRLRTEQSFYSDSISHMV
                     HADSNGFLEGETKTIYSLGVSMVADDAKSTSDGAGDSTNIGRKQTDGWYSAARFHKNL
                     TSSREIVKKACHRTLRKLGAVKPASQEVPVVFSSEMAQSFLGKIASALMGENLFRKQS
                     FLLNRLNESIADSKINLIDNPLLPGKLGSRHFDSEGVEAKPLVLIENGVLKNYMLSTY
                     SANKLGMTSNGHSGGISNLILNLGDYPEEELIASVKNGLYLTFMSGQGANIVTGDFSR
                     GAQGIWIREGKLAEPVSEFTIAGTFTEMLNNLKMIGSEVDERSSILTPSFKIDKMAVS
                     GT"
     gene            complement(1571..1951)
                     /locus_tag="EYO68_07105"
     CDS             complement(1571..1951)
                     /locus_tag="EYO68_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07610.1"
                     /translation="MNVNQTKSAIPLNFTEKGNGTLSSVSGSQRLSFENIRSFRQHFE
                     QGTIPMRFALGDNQHQTLIVAMYEGTEKLLNQDVSEFAVLEYLEGLKLTAKELRQQGI
                     SGEQFRTSLLEHSASTVRDLVSSS"
     gene            2047..3096
                     /locus_tag="EYO68_07110"
     CDS             2047..3096
                     /locus_tag="EYO68_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIC07611.1"
                     /translation="MNNPRKILVIRFSALGDLVLTSPIFRELKRIYPDLGITLLTSSR
                     QGTVLDNNPHIDQVIRYSRNGSGVLLKTLIQKLRRERYDLIYDAHRSLRSIWIVWNLS
                     GFGLFKYPQVWSINKRSWKRDLLIRWKLNFLKKTPSQRQHLLRPLQEHTKLELNNRTE
                     LFPDKSAVLLIQEFLKKNNFLPKRFVAIGPSASYPLKCWPLVYFNEVISSLLEQGWSV
                     VLVGGTGEKETIQLEKEFSGKVQSVAGRFSPLETAELLRQASIVVTNDTSVGHLAESM
                     RTPVIVLFGATVREFGYAPFLEESKMLETEEVLGCRPCSRDGRGKCRNPDYLRCLTTI
                     TPEMVLSLIPKTKTN"
     gene            complement(3477..5414)
                     /locus_tag="EYO68_07115"
     CDS             complement(3477..5414)
                     /locus_tag="EYO68_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07612.1"
                     /translation="MNTKIFSRIQKRKFMGLKRVFQSILVSSIILFLLNPALSLEAQE
                     RVKSLSVKEFEIEEAIHNGLKYYLDTKDYVLRVKLFGEERSIGSTADEEALPGFGQLN
                     DPVGSGATYWEITRMRVDLVMHKEVSPSVNTYIGEIVPILSGLDYERGDELIFVPILP
                     SDPFPEQVGDPSSAGLPDPMAKALPEDDPKDIEVALQSGEPSEEQDGESLDEEPFWLR
                     MSDIEKILAGMLLFLILLFFWVLWKLRKFKAISTEQEGFPALMPPQSETPQLPSSPNQ
                     GFVSIADTNAKLLNGHENHENQLNDAVLIADNERLIQEIIKQMIGRDDWKQELVQEMS
                     RDKQSMDMLTQLIATLGMSTSRKLFSDTIPQNTYLDLEKLAEEASSSPEEENEMLKDI
                     QKFFLTKQLTDPEQNKSNPFSFMDQLSTSQIGFLVKDEPAKIKAFIFGRLESDEAAEL
                     LRDLSKDERTKVALELGKLHELPLELVEKIGYNLAEKARHVPDDGTVGVDGIKFIADV
                     LGDLDNTTRQELINGLRTSDIKLSENIESHCFIFESIPVVPKDILLEVVRKLQPDDVI
                     TAISGTTSKIKEAAIMCFPEKSRPALVSSLKTKSPDSDEVRAARKLFVQSMRDMSDAG
                     RLNLQEVNTKFTQESSQTESA"
     gene            complement(5417..>5808)
                     /locus_tag="EYO68_07120"
     CDS             complement(5417..>5808)
                     /locus_tag="EYO68_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC07613.1"
                     /translation="TDNKKPDAGEGELELAEQKKDNETLEDKPEAILEPDPEPRRIDQ
                     KTRVETARYLLQVRKAYLKEDYDAAITALRRAIELNPFSSQAFAMLGSVYYRLGWNRM
                     AIENWQHSLELDPTNEGLKKYLMRLSR"
BASE COUNT         1670 a         1147 c         1183 g         1808 t
ORIGIN      
        1 cggagctagg gattaggaat cccctatttt atccagttaa actatgggag ccgattgtgt
       61 ttaggaaaaa tagctgataa atattaagca atgtttcgtt agcataaatc aagacaacat
      121 ttattatttt tgactcagga acaggtctcc aaatttgaaa aatctgactg aagattcact
      181 ggcacgataa gcagacaaac tcacgatatg ttagaaaaat ataaatctat tactaaagaa
      241 attattgatt tggctaaaca atatggagca gaacaagcat cagtttcact tgccaactca
      301 acgtcttttc aggttgatgt acgtgaggaa aaaattgaat cacttcagga atcaggttca
      361 tccggggttc atctcacagt ttcaaaaaac aaacgcagat ctactgtttc ttcaaatgat
      421 ctgcgaattg agacactggg ccctttaatc cgtacaacta tggatgcact cccttttatg
      481 ggtgaagatg agttttacag ccttcctgac cctgcgttac aaggacgtgc accgggtaat
      541 ctggaatttc ttgatccgga ttttaacaag ttgagctctg aagaaaaagt gcaaattcca
      601 ctcgatttgg aacagttgac tctgaactct gatagaaggt taagaacaga gcaatcattt
      661 tattccgaca gtatttctca catggttcat gcggattcca acggctttct tgaaggcgag
      721 acaaaaacta tttatagtct tggagtttcg atggttgcag atgatgcaaa atcaacttct
      781 gatggagccg gtgactcaac taacattgga cgtaaacaaa cggacggttg gtacagtgca
      841 gctcgttttc acaagaatct gacttcctcc cgggaaattg taaaaaaagc ctgtcataga
      901 accttacgca aacttggtgc agtcaaaccg gcaagccagg aagtccctgt agtttttagt
      961 tcggaaatgg ctcaaagttt tctgggtaaa atagcctctg cattaatggg cgaaaatctt
     1021 tttcgcaagc aatccttcct cttgaatcgg ctaaatgaat ctattgcaga ttctaaaata
     1081 aacctgatag ataatccttt acttccagga aaacttggga gtcgtcactt tgacagtgaa
     1141 ggagttgagg ccaaaccttt agttttgata gaaaatggtg tactgaaaaa ttatatgctc
     1201 tctacatatt ctgcaaataa acttggaatg acttccaacg gtcactctgg aggaatttcc
     1261 aacctgattc ttaatcttgg tgattatccg gaagaggaat tgattgcaag cgtcaaaaac
     1321 ggattgtatt tgacctttat gtcaggtcag ggagcaaata ttgttacagg agatttctct
     1381 aggggggcac agggaatttg gattcgtgaa ggaaaattgg ctgagccggt aagtgaattt
     1441 actattgcag gtacttttac tgaaatgttg aataatttga aaatgattgg cagtgaagtt
     1501 gatgaaagaa gctcaattct cacaccatct tttaaaattg acaaaatggc agtgagcgga
     1561 acctgataaa tcaggaagag cttaccaagt ctcggacagt tgacgcagaa tgttcaagca
     1621 gagatgttct gaattgttcc ccggaaattc cctgttggcg caattctttg gctgtcagtt
     1681 taagtccttc cagatattcg agtacagcaa attcagatac atcctgattt agtaattttt
     1741 cggtaccttc gtacattgca actatcaaag tttgatgttg attgtctcct aaggcaaagc
     1801 gcataggtat agttccttgt tcaaaatgtt gacgaaaact gcgaatattt tcaaatgaaa
     1861 gtctttgact tcctgataca gaagaaagag ttccattacc tttttcagta aaatttagtg
     1921 gaattgcaga ttttgtttgg tttacattca ttgtattgta atgattagta gtggatttga
     1981 cgttcttata ttcaaaacaa gtgtgatgca aacaccatga aattttcact caattactaa
     2041 aggttaatga ataatccccg gaaaattctg gtaattcgtt ttagtgcact gggtgatttg
     2101 gtgctgactt caccaatatt tcgagaattg aagcgtattt atcctgattt aggaattaca
     2161 ttgctgactt catcaagaca aggcactgtc cttgataaca accctcacat tgatcaagtc
     2221 attaggtaca gtagaaatgg atccggtgtt ctgctgaaaa ccttgataca aaaattgcgg
     2281 agggaaagat atgacctgat ttacgatgct caccgttctt tgagaagtat ttggattgtc
     2341 tggaacctct ccggattcgg gctttttaaa tatccgcaag tttggtccat caacaagcgc
     2401 agttggaaac gtgatttact cattcgttgg aaactaaatt ttctcaaaaa aacaccatct
     2461 caacgtcagc acctgcttcg tcctttacag gaacacacaa agttggaatt gaataaccgg
     2521 actgaactat ttccggacaa aagtgctgtt ttactaattc aggaatttct aaaaaaaaac
     2581 aactttcttc ccaaacggtt tgttgctata ggtccgtctg catcctatcc tctgaaatgc
     2641 tggccactgg tttattttaa tgaagtgatt tcaagtttgc ttgagcaagg ttggtctgta
     2701 gtactggtcg ggggtacagg tgaaaaagaa actattcaac tggaaaaaga attttcaggg
     2761 aaggtacaaa gcgtagcagg ccggttttct ccacttgaaa ctgctgaatt gcttaggcaa
     2821 gccagcattg ttgttactaa cgacacttct gttggtcacc tggcggagtc gatgcgtacc
     2881 cctgttattg ttttgtttgg agcaactgtg agagagtttg gctatgctcc atttttggaa
     2941 gaaagtaaaa tgctggaaac agaggaagta ctgggttgcc gcccatgttc ccgtgatggc
     3001 cgcgggaaat gccgtaatcc ggactatttg cgctgtttaa ccacgattac tccggaaatg
     3061 gttttgtccc taattccgaa gactaaaact aattagtgct tgctgaaaaa gtttttcttt
     3121 tgtcatcccg gacttgatcc aggatccaaa tcgtcgttaa gtttgagtac aagatttgaa
     3181 acattttccc tggattccgg atctatgcag cgcttcgccc ggaatgtcaa atggcaagaa
     3241 tcgaaagaag caaaattatt gttctcgtga ggtttccttg ttgaataatg gactcaaacg
     3301 acagaagtgc agggctttcg tgctttttca ggaaacacca tgcagtttac ttcatcaatt
     3361 gaataattac ttattgatat tattgaaaag tagtattttt cagcaagtgc aaaatatatt
     3421 gaatataacc aggccccggg agcaaatctt ccagtttcct aagatgttaa ttattgttac
     3481 gcagattcag tttgactgct ttcttgagta aactttgtat tgacttcctg taggttcaac
     3541 cggccggcat cagacatatc ccgcatagat tgtacaaaca acttgcgggc tgcacggact
     3601 tcgtctgaat ccggtgattt cgtttttagt gaagaaacga gggcaggacg gcttttttca
     3661 gggaaacaca tgattgctgc ttccttgatt tttgaggtag taccagaaat tgcagttatc
     3721 acatcatcag gttgtaattt tcgcacaact tccagcaata tatcttttgg aacaactggt
     3781 attgactcaa aaataaagca atgactttca atgttctcgc tgagttttat gtctgaagta
     3841 cgcaagccgt taatcagttc ctgcctggtt gtattgtcca aatcaccaag gacatctgca
     3901 atgaatttta ttccatcaac tccaactgta ccatcatcag gcacatgtct ggctttttct
     3961 gcaaggttgt agccaatctt ctccaccagt tcaaggggaa gttcgtggag tttccccaat
     4021 tccaatgcca cttttgtacg ctcgtctttg gataaatcac gtaataattc agcagcctca
     4081 tcactttcca aacgcccaaa tataaaggct tttatttttg caggttcgtc tttaaccaaa
     4141 aaaccgattt gtgatgtact aagctgatcc atgaaactga atggatttga tttattttgt
     4201 tcgggatcag ttaactgttt ggtaaggaaa aatttctgta tgtcctttaa catctcattc
     4261 tcttcttcag gagaagaact ggcttcttcc gctaattttt ccagatccaa ataagtattt
     4321 tgaggaatag tatctgagaa aagttttctt gatgtactca ttcccagagt agcgatcaac
     4381 tgggtcagca tgtccatcga ttgtttgtcc cgagacattt cttggactaa ttcttgtttc
     4441 cagtcatcac gaccaatcat ctgtttaata atttcttgaa tgagtcgctc gttgtctgcg
     4501 attaggacag catcatttaa ctggttttca tggttttcat gaccatttag gagtttagca
     4561 ttagtatccg caattgaaac aaaaccttga ttaggagacg aaggtaattg aggcgtttca
     4621 gattgaggcg gcatcagggc aggaaaacct tcttgttcgg ttgagattgc tttgaatttt
     4681 cttagtttcc agagaaccca gaaaaacaaa agaataagaa acaacaacat tccggcaaga
     4741 attttttcaa tgtcgctcat tctcagccaa aacggttctt catctagtga ttctccatct
     4801 tgttcttcag acggttcacc actctgcaat gcaacttcta tatcttttgg gtcatcttcc
     4861 gggagggctt tcgccattgg atccggtaaa cctgctgaag aaggatcacc aacctgctca
     4921 ggaaatggat cactaggcaa aattggtaca aaaatcagtt cgtctccacg ctcataatca
     4981 agtccactga ggataggtac aatttcaccg atgtaagtgt ttactgaagg tgaaacttct
     5041 ttgtgcatca ccaaatctac acgcatccgt gtgatttccc agtatgtagc acctgaacct
     5101 acaggatcat tgagttgtcc gaatcctgga agcgcctcct cgtctgctgt actaccgatg
     5161 gaacgttcct ctccaaacag ttttacccgc agtacgtaat ccttagtatc caggtaatat
     5221 ttgagaccat tgtggattgc ttcttcaatt tcaaactcct tgacacttag cgacttgacg
     5281 cgttcctggg cttcaaggga taaggctggg ttgagcaaaa ataaaattat tgaggatact
     5341 aaaatagatt gaaaaaccct tttcagtccc atgaatttgc gcttctgtat ccttgaaaat
     5401 atttttgtat tcattgttac cttgacaaac gcatgagata ttttttcaga ccttcatttg
     5461 ttggatcaag ttcaagcgag tgctgccaat tttctattgc cattctattc catccgagac
     5521 gatagtaaac tgaacccagc atagcaaaag cttgtgagga gaagggatta agttcaattg
     5581 ctcttctaag agcagtaata gcagcatcat aatcttcctt caaatatgct tttctaacct
     5641 gtaacaaata acgtgctgtt tcaacccgag tcttttggtc tatccggcgg ggttctggat
     5701 caggttccaa tatagcttcc ggcttatctt caagtgtttc attgtctttt ttctgctctg
     5761 ccagctcaag ttctccctca cctgcatccg gctttttatt atctgttt
//
LOCUS       DTVD01000208            5766 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4500_length_5766_cov_180.721065, whole genome
            shotgun sequence.
ACCESSION   DTVD01000208 DTVD01000000
VERSION     DTVD01000208.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_5766_cov_180.721065"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="EYO68_07125"
     CDS             <1..620
                     /locus_tag="EYO68_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIC07614.1"
                     /translation="IREWEWMEDNPMRKISKLKEPRGRVRHLSKDERESLLAACKDSV
                     NNHLYLAVVLALSTGARQQEIWGLQWSEVNFETGFITFTETKNEEFRSVPLVELGREL
                     LLDHYRLRREDSDIVFPSKKNPAVSYDFRNPWKKALVVAEVEDFRWHDLRHSCASYLA
                     MNGVPMRTIAEILGHKTLSMVQRYTHLNAEHLTEAISDMNRKIFP"
     gene            629..1525
                     /locus_tag="EYO68_07130"
     CDS             629..1525
                     /locus_tag="EYO68_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07615.1"
                     /translation="MAKPKIINSNPKRTISFSNFNRLDKGLKIAIGLKWSKLIINDVA
                     EDKIELVEDWINNNRGKYKGINIRIGRTYTRQLVLIFSGIDELKEGLSSAIKRKFREI
                     ILEDVSEEEIKPIQNWIKKNQGKYEDINIRIGVTYKGLHPNEKEKIRNKVGADVSFFD
                     DSTLHLEENGEKLFFDNWINSESPQKRIDRIEREIKHQQKTYKIKGIEKIEKKKIKLE
                     LKYLKQLLDQNKIEIEIFDELKKQTEKDISGNAHRRRKRWTDKKRLRLIVRVIDKKLW
                     EENGKKPTRNDVEIELLIDSKM"
     gene            1800..2309
                     /locus_tag="EYO68_07135"
     CDS             1800..2309
                     /locus_tag="EYO68_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIC07616.1"
                     /translation="MAGRMTLNPIVHLDLFGSLMLLMVGFGYARPVPVNPRNYRISNA
                     DFYVASAGPIMNLLLGFGAAFLFRFLALNNLTLLAGVPVLEILYLFILINFNLCLFNL
                     IPLGPLDGNSVFPHFLPSDLRRRFQNWNYRYGSQALIVLVLLSMFLPGFSAFSWISSI
                     SKSMISGLL"
     gene            2373..3728
                     /locus_tag="EYO68_07140"
     CDS             2373..3728
                     /locus_tag="EYO68_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIC07617.1"
                     /translation="MTLEELKSLFLGYCRKEDGTPDKQLFGLEYENFVMVPNNDDGEE
                     CFHPLPVEGESGVFRVLENLVELTKNSDDPLEKVYEKDMLLALISPAGSKITVEPGGQ
                     IELSDAPRKSLLDAYNSLQDYLKLLEKAVSDFKGKLLFQGVQPIHSLDKLPFFPKNRY
                     RIMFQHMLKTGTLGQWMMKGSSGVQVSIDYASLEDLQRKFIFLNRLSPFLTAMFANSP
                     LNAGSPCGFLSYRSHIWEKTDNSRCGLPEIFLRDNFRLEDYINWSLKAAPYHLMREGE
                     VVETTDWNFKQLLEGKHPDLIITPEDWEDHLGMLFPDIRIKNILEVRVVDSVPPKYTM
                     AIPALIGTLLYNESAFSSAQSLLMDLPQDEFQLYKKAAAKDGLQAEVNQTNFAKTGRK
                     LAEIALQELGSKDENWFLPFFEEFTKEGRSPANETLDQFRECGEDPDKWLAKVLNNPD
                     Y"
     gene            complement(3722..4666)
                     /locus_tag="EYO68_07145"
     CDS             complement(3722..4666)
                     /locus_tag="EYO68_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIC07618.1"
                     /translation="MLHRTEIPVKTFELAEVHLTTGRHQLMDVVFRRWASLLQDRLYA
                     KKNLMFEVSTENVKKIRFENFLSSVSKQPIYIFETPNQSRGLFLVDNTFFLKLILNTK
                     AGITLERSSLGKLMKEHQKDLLALVQPMIEDFEKSWLNIADVELNLNRVTIYPQRAKV
                     MLPYEYCFVGSVRLSVQDIQSEILLCLPSSGLDPLLSHLENKRVIAPESIENYSPHVK
                     RHFMDILEKKEYSVVAELGNADLGETKGKLEVGQLLPLLGKENLATIRINGTPALQGY
                     TGESDGHYSVQVTRGIEDKKPSAIQEEMAFKQVTWPSQ"
     gene            complement(4675..>5766)
                     /locus_tag="EYO68_07150"
     CDS             complement(4675..>5766)
                     /locus_tag="EYO68_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIC07619.1"
                     /translation="LDELDGDESEENSDSDDSFVDDIDGEIDKALDVGESEASDDAEN
                     SGESTEEEATELPEASEAGEPEIVDDVENSGESTEEEVEASESSEPEAFDDVENSGES
                     TEEEVEELDLSDNMDLSEDLDPEELTDSSGETDAVEESGESLPAEEADTESSEVLEML
                     HDPEISEDSEESEANDTEDLELGIDALDDIKDSEEADTEQENAETDLVDIGESEIDEL
                     KFDNETEENSASETSADSQEENEVTGEQMDASDSVEVEPEIEKTPVIEKVESEMPIET
                     EASETSAVPLDADMLLKFHHEAVVEIARTQLTGEEITQITYGSIIELDKVAGEPVNLV
                     LDGKTIALGEVIQINNDKLGIRIVGIVQE"
BASE COUNT         1771 a         1109 c         1104 g         1782 t
ORIGIN      
        1 ctattaggga atgggaatgg atggaggata acccaatgcg aaagatttca aaattaaagg
       61 agcctagagg aagagtccga catttgtcta aagatgaaag agaaagctta cttgctgcat
      121 gtaaagattc agtaaataat catctttatt tggccgttgt tttagctttg agtactggag
      181 ctcgccaaca agaaatttgg ggtctccaat ggtcggaggt aaattttgag acaggattca
      241 tcacttttac tgagacaaag aatgaagagt ttcgaagtgt accactagta gagctcggtc
      301 gtgaattgct tttagaccat tatagactcc gaagagaaga ctctgacata gtatttcctt
      361 caaagaaaaa tccagcagta agttatgatt ttagaaatcc ttggaaaaaa gctcttgtag
      421 tagcagaggt tgaggatttt agatggcatg atctcaggca ttcatgtgcc tcttatctgg
      481 ctatgaatgg agtgccaatg cgcactattg cagagatatt agggcataaa acactatcga
      541 tggtgcagag atacacacac cttaacgcag agcatttaac ggaagcaata agcgatatga
      601 atcgcaaaat ttttccataa aaagatatat ggctaaacca aaaataataa attctaaccc
      661 aaagagaaca attagtttta gtaattttaa tagacttgat aaagggttga aaatagcaat
      721 agggctaaaa tggagcaagc tcatcattaa tgatgttgct gaagataaaa tagaattagt
      781 ggaagattgg atcaacaaca atcgaggtaa atataaagga attaatatta gaataggtag
      841 aacatacact cgacagttgg tcttaatttt tagtggcatt gacgaactta aagaaggact
      901 gagtagtgca ataaaaagaa aattcagaga gataatatta gaggatgtct ctgaagaaga
      961 aattaaaccc atacaaaatt ggatcaagaa aaatcaaggt aagtatgaag atattaatat
     1021 tcgcataggc gttacataca aaggactgca tccaaatgag aaagagaaaa taagaaacaa
     1081 agtaggtgct gacgtatctt tttttgatga ttcaacatta cacttagaag aaaatggcga
     1141 gaaactattc ttcgataatt ggattaattc cgaatctccc caaaaaagaa ttgatagaat
     1201 tgaaagagag ataaaacatc agcagaagac ctataagatt aaaggaatag aaaagataga
     1261 aaagaaaaaa ataaagcttg aattgaagta tctcaaacaa ctactagatc aaaataaaat
     1321 tgagattgaa atttttgatg aactaaaaaa acaaacagaa aaagatattt caggaaatgc
     1381 ccatcgtaga aggaaaagat ggactgacaa aaaaaggtta cggttaattg tccgtgtgat
     1441 tgacaaaaaa ctttgggaag aaaatggaaa gaaaccaact cgtaatgatg tagaaattga
     1501 actactcatc gacagtaaaa tgtaaatcac ctggaaatga gtggttagaa ttaaactgag
     1561 aaaagaactg agatgaaata tttgtctttt agtggcattt aattttctga tataattaag
     1621 cgtaaaagtc caaattttct atccatgatc acacgtttct gctgctagaa tcatctgtgt
     1681 tgtttagcct gccaccacaa gttttaatca ttctagtttt ctgtttgata tttgctctca
     1741 ctttccatga gttcggccat gcttatgtag cccatctttg tggagacgac acggcaaaaa
     1801 tggctggtag aatgacactg aaccctatcg ttcatttaga tctctttggc agtttaatgt
     1861 tgctcatggt aggttttggc tatgcccgtc cggtgccggt taatcctaga aattaccgta
     1921 tcagcaatgc agatttttat gttgcttctg cggggcccat tatgaattta ctgttaggat
     1981 ttggtgctgc ttttttgttt cgctttctgg cactgaataa cttgacacta ctggctggag
     2041 taccagtact ggaaatcctt tacctgttta ttttaattaa ttttaatctt tgccttttca
     2101 acttaattcc acttggtcca ctggacggaa attcagtgtt tcctcatttt ttaccttcag
     2161 acttaagaag acgtttccag aactggaatt accgttatgg ctcacaggcc ttgatagtgc
     2221 tggtattatt aagtatgttt ttgcctggat tcagtgcttt cagctggatt tcgagcatta
     2281 gtaaaagcat gattagcgga cttctttaga ttgccacttt tcaacttctg gaatcctcag
     2341 acacaacact aaatgtttta taataattac aaatgacgct tgaagaatta aaatcacttt
     2401 ttctagggta ttgccgtaaa gaggacggta ctccagacaa acaacttttt ggcttggagt
     2461 atgaaaactt tgtgatggtt cctaacaatg atgacggtga agaatgtttc cacccgttac
     2521 ctgttgaagg agaatccgga gttttcaggg ttttggaaaa tttagtagaa ctgaccaaaa
     2581 acagtgatga tccactggaa aaagtatatg aaaaagacat gcttttagca ctgattagtc
     2641 ctgcaggatc aaaaataacg gttgagcctg gaggacaaat tgaactttct gatgcaccaa
     2701 gaaaatctct tttagatgca tataactcat tacaggatta tttgaagctg cttgaaaaag
     2761 cggtttcaga ttttaaagga aagcttttgt ttcagggcgt gcagccgatc cattctttgg
     2821 ataaattgcc gttttttcca aaaaaccgtt atcggattat gtttcaacac atgttgaaaa
     2881 ccggtacact cggtcaatgg atgatgaaag gatcttctgg agtacaggtc agtattgatt
     2941 atgcttcctt agaggatttg cagagaaaat ttatttttct gaatcgcctc tcaccttttt
     3001 tgacagctat gtttgctaat agtcctttga atgctgggag tccctgcggt tttctttctt
     3061 atcgctcaca tatttgggag aaaacggata attcacgttg cggtcttcct gaaatctttt
     3121 taagagacaa tttccgtttg gaggactaca tcaattggtc attaaaggca gcaccatatc
     3181 atttgatgcg tgaaggtgaa gttgttgaga caactgattg gaatttcaaa caacttttag
     3241 aaggaaaaca tccggatcta ataattactc ctgaagactg ggaagatcat ttaggaatgc
     3301 tgtttccaga catcagaatt aaaaatatcc tggaagtgcg tgttgttgac agcgtaccgc
     3361 ctaaatatac aatggcgatt ccggcattga taggaacatt gctctataat gaatcagctt
     3421 tttcttccgc acaatcttta ctaatggacc tgccgcagga tgaatttcaa ttgtacaaaa
     3481 aagctgctgc aaaggatgga ttgcaggcag aagttaatca aactaacttt gcaaagacag
     3541 gaaggaaact ggcagaaatc gctctgcagg aacttggatc aaaagatgaa aattggtttc
     3601 tgcctttttt tgaagaattc accaaagagg gtcgatctcc ggcaaacgag actctggacc
     3661 agttccggga atgtggagaa gacccggaca agtggcttgc taaagtctta aataacccgg
     3721 attactgact gggccaggtt acttgtttaa atgccatctc ctcctgaatt gcggaaggtt
     3781 ttttgtcttc aattccacga gtcacttgaa cagaatagtg accatcagat tctccagtat
     3841 aaccttgtaa cgcaggagtc ccgttaatac gtattgtcgc cagattttct ttacccagta
     3901 acggcagtaa ctgtcccact tccaactttc cttttgtttc gcccaaatct gcattcccca
     3961 attctgcaac aaccgaatat tctttctttt ccagaatatc catgaaatgc cgtttaacat
     4021 gcggagaata attttctatg gattcaggcg caataactct tttgttttca agatgactaa
     4081 gcagtggatc aagccccgaa gaaggtaaac agagtaaaat ctctgattga atatcttgaa
     4141 cactaagccg aacactaccc acgaaacaat attcgtaggg caacatgact tttgcccttt
     4201 gaggataaat ggttacacga ttgagattaa gctccacatc tgcaatattg agccagcttt
     4261 tttcaaagtc ttctatcata ggttgcacca aagcaagtaa atctttctgg tgctccttca
     4321 tcaacttgcc cagggaagaa cgttcgagtg ttattcccgc ctttgtgttc aagatcaact
     4381 ttaaaaaaaa tgtattgtct acaaggaaca gaccacggct ctggttcggc gtttcaaaaa
     4441 tatagattgg ctgtttagat acagaactca aaaaattttc aaaacgtatt tttttcacat
     4501 tttccgtaga gacctcaaac atcaggttct ttttagcata aagcctgtcc tgtaagagag
     4561 atgcccatct gcggaataca acgtccatta attggtgtcg ccctgttgtc agatgaactt
     4621 ctgccagttc aaaagtcttt acgggaattt ctgttcggtg aagcatagtt taaattattc
     4681 ctgaacgata ccaacaattc gaataccaag tttatcattg ttgatttgta taacttcccc
     4741 aagagcaatt gttttaccgt cgagtaccaa attcaccggt tctcctgcaa ctttatctag
     4801 ttcaataatg ctcccataag ttatctgtgt gatttcttcg cctgtaagtt gagtacgtgc
     4861 tatttcaacc acggcttcat ggtgaaattt taacagcatg tctgcatcta aggggactgc
     4921 tgatgtttct gatgcctcag tttcaattgg catctcgctc tctacttttt caataacagg
     4981 tgttttctca atttcaggct ctacttctac ggaatcagat gcatccattt gctcacctgt
     5041 tacctcattc tcttcctggg aatctgctga tgtttctgat gcagaatttt cctccgtttc
     5101 attatcaaat tttagttcgt caatttcact ttctccgata tcaaccagat cagtctcagc
     5161 attttcctgt tcagtatcag cctcttcaga atcttttatg tcatccaaag catcaattcc
     5221 aagctccaga tcttctgtat catttgcttc actttcttca ctatcttcag aaatctcggg
     5281 atcgtgcagc atctccaaaa cctcagacga ctcagtatcg gcctcctcag caggaagact
     5341 ttctccggat tcttctactg catcagtttc acctgatgaa tctgtcaatt cctctggatc
     5401 aagatcttcg gacaaatcca tattatccga caaatctaat tcttccactt cttcttctgt
     5461 actttctcca gaattttcta catcgtcaaa tgcctctggc tcactggatt ctgatgcttc
     5521 tacttcttct tctgtacttt ctccagaatt ttctacatcg tcaactatct ctggctcacc
     5581 cgcttcggat gcttccggca actctgttgc ttcttcttct gtactttctc cagaattttc
     5641 tgcatcgtct gatgcctcag actcacctac atctaatgct ttgtcaattt ctccatcaat
     5701 atcatctaca aaagaatcgt cagaatcaga gttttcttcg ctttcgtcac catccagttc
     5761 atccag
//
LOCUS       DTVD01000209            5764 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4504_length_5764_cov_374.018917, whole genome
            shotgun sequence.
ACCESSION   DTVD01000209 DTVD01000000
VERSION     DTVD01000209.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_5764_cov_374.018917"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(13..1758)
                     /locus_tag="EYO68_07155"
     CDS             complement(13..1758)
                     /locus_tag="EYO68_07155"
                     /inference="COORDINATES: protein motif:HMM:PF02875.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07620.1"
                     /translation="MKLTLESCRRLTGPSLYFQNPGTVLDVFVSGVRLEDVVACWQEE
                     VNKLREAVSWKLSPLHVRKTHPQGASLAFNASMDLVYSATELNEAAWNNSVARLYGEV
                     PEDFDGIVSKLKAQIEQESNPVLLKLNEAANKKGIHLMSDDETLTLGLGVGSQSWSLG
                     ELPSPDEVDWNALHNIPVTLITGTNGKSTSGRMLAAIVSADGKTPGLTSTDCIQVGTD
                     ILDTGDYSGPGGALAVLSDKRVEFGVLESARGSMLRRGLGVTQAQAGIITNVAEEHLG
                     EYGVLSLADLVDVKFTILRPLEVSGGTLVLNADDAGLVEYAKNVNHPLCWFSLEKDSH
                     ILYEHRKQGGPICYVEQGKIYFLHHDSVKFILNVNEIPITLNGAARYNVANSLGVIAL
                     AKNLGFSNEVIAGALKSFQGDLKDNPGRGNYLTLPLPKPNRSQNSTEEQSFQVLIDFA
                     HNAHGLSAICETLIRLPAKRRLVVAGHVGSHSDEEIREMTRVVAKIKPEYFYSPEMLD
                     YLRGRKAGEISKLVFDELRQLGYAEEKLSTANTCLEAVKSAVEHVQAGDLLMLVGLDE
                     RKETLAYLEELKLQI"
     gene            complement(1788..4595)
                     /gene="cphA"
                     /locus_tag="EYO68_07160"
     CDS             complement(1788..4595)
                     /gene="cphA"
                     /locus_tag="EYO68_07160"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HIC07621.1"
                     /translation="MKILSTNVFQGPSLYAHFPVIRHVLDLGVLEEWPSTRIGQDYVD
                     QLVEILPGLKEHGCSYRIPGGFIRRLREDDGTWMGHIMEHVALELQGIAGSEVTFGRT
                     RSTGNPGEYNMVFQYMQRDVGLKASELALRLLLCLLPDSLKKQLDTPPSADFNFEEER
                     DAFIRFAQRHEFGPSTASLVNAAKERGIPYLRLNRFSLVQFGHGKYQRRIQATITSET
                     RQIAVVIAGDKEETHTILYDLGLPTTMQRLAYSEEEAVSQAENLGFPVVVKPLDGNHG
                     RGVSINLISDEQVVLGFNEARAQSNNEAIIIENYVTGFDHRMLVVNGELVAVSKRVPG
                     HVVGDGEKTIEELVEIVNSDPRRGIGHEKVLTRLKFDEQAERLMKESEYSSQTVLTKG
                     EIFYLRSTGNLSTGGTAIDLTDVVHPDNREMAVRAVKSIGLDVGGVDFLTDDITQSYK
                     DIGGAIVEVNSAPGFRMHIAPSEGKPRDVATSVMDMLFPPGSPSCIPIAAITGTNGKT
                     TTARMLSYIMKTSGNTVGMTSTDGVYIDGHLTVKGDMTGPVSSQMVLRDPSVDVAVME
                     VARGGMLKKGVGFPKCDVAACLNVSADHLGLRGIDTVEQLARVKRIIIEIATDTAVLN
                     ADDSLCLEMANHTKAENICYVTLADNHPLVRKHIRAEGRAVVLEKGLNGDMITLYDKG
                     AHIPLLWTHLIPATLEGKAVHNVQNAMFAAALSFSMGVPLDDIRHGLRTFNTTFFQAP
                     GRMNIFDDHPFKVILDYAHNPAAFKGMCHLVSRMETPGKNICVISMPGDRRDEDIQEV
                     GKTVSGVFDHFICKADDDRRKRGYDEIPKMLRKILLKNGVSEDCIDVVPDEVESIHRG
                     LTLAQPGDLLMVFGDNVSRSWKQIVHFNEDLELPSIQPKQQPKEEDSVEAFVEPFTMV
                     AGQRIIQDERGVRLASEEEAD"
     gene            complement(4639..5454)
                     /locus_tag="EYO68_07165"
     CDS             complement(4639..5454)
                     /locus_tag="EYO68_07165"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006991989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HIC07622.1"
                     /translation="MASHVAEGSQRGYIIPIGGAENKRRKPLILQKFAELCGGKNARL
                     AIIPTASKLKETGQNYIDIFRDIGVDYCVSLPIEERSDSECVEYLQELNEASGIFVTG
                     GNQLRLSTIMGGTPVAQAIRRRNAVGVHFAGTSAGAAIIPEHMIAGGKAGPTPREGGV
                     IFAPGLGLTNKIVVDQHFRQRDRLGRLLSAVAFNPFATGIGIDENTAAFIDPEGMLEV
                     VGSGAITVVDPSELSHSSMESENRGKAINLTNLRLHLLTSGAKYDINSRQVFK"
BASE COUNT         1562 a         1445 c         1164 g         1593 t
ORIGIN      
        1 gtttgagaca ttttatattt gcaatttaag ttcttcaaga taagcaagag tttcttttcg
       61 ctcgtcaaga ccaaccagca tcagcaggtc gcccgcctga acatgctcaa ctgcagattt
      121 tacggcctcc aggcaagtgt tagccgtgct gagtttttct tcagcatatc ccagttgccg
      181 taattcatca aaaaccaatt tggagatttc tcctgctttt ctcccccgta aataatccag
      241 catttcagga gaataaaaat attccggttt gattttggca accacacgag tcatttcccg
      301 tatttcttcg tcgctatggc tgccaacatg accagccaca accagccttc gttttgcagg
      361 aagcctgatg agagtttcac aaattgcact caatccgtgt gcattatgtg caaaatctat
      421 caacacctga aaggattgct cctctgttga attttgagaa cgatttggtt tgggaagagg
      481 aagtgtcaga taattgcctc gcccgggatt atccttgagg tcaccctgaa agctcttaag
      541 tgctcctgca atcacttcat ttgagaaacc caaatttttg gccaaagcaa taacaccgag
      601 agaattggct acattataac gtgcagcacc atttagagta atcggaattt cattcacatt
      661 caggatgaat ttgacagaat cgtgatgcag aaaatatatt tttccctgtt caacataaca
      721 aatgggacct ccctgtttcc ggtgttcata caggatatga gagtctttct ccagactgaa
      781 ccaacacaaa ggatggttta catttttggc gtattccacc agtcctgcgt cgtctgcatt
      841 gagaaccaga gtgcccccag atacttcaag aggtcggaga atggtaaatt tgacatctac
      901 taggtcggcg agactcaaga caccgtattc gcccaaatgc tcttcagcta cattagtaat
      961 aattccggcc tgagcctgag tcactcccag cccccgacgt agcatgctgc cacgcgctga
     1021 ttcaagcaca ccaaattcca cacgtttatc actcagtacc gcgagtgctc cccccggtcc
     1081 ggaataatct cctgtatcta gaatatcagt tccgacctgg atgcagtcgg tagaagtcag
     1141 tccaggagtt ttcccatcag cactgacaat agcagctagc attctaccgg aggtagattt
     1201 gccattagtt ccggtgataa gtgttacggg gatgttgtgg agagcattcc aatccacctc
     1261 atccggactg ggaagctccc ctaaagacca gctttgtgag cctactccaa ggccaagtgt
     1321 aagggtttcg tcatcagaca tcagatgaat gccttttttg tttgccgcct cattaagttt
     1381 tagcaggact gggttgcttt cctgctctat ttgcgctttg agttttgata caataccatc
     1441 gaagtcttca ggaacttcac cataaagccg tgcgactgag ttgttccaag ctgcttcatt
     1501 aagttcagtt gcagagtaaa ccaggtccat agacgcattg aaggccaaac tcgctccctg
     1561 tggatgtgtt ttcctgacat gcaaaggtga cagtttccaa gagactgcct cccgtaattt
     1621 attaacttct tcctgccagc atgccacaac atcttccaac ctaaccccgg aaacaaaaac
     1681 gtcaagtaca gttccaggat tctggaaata cagacttgga cccgttaaac ggcgacagct
     1741 ttcgagtgtt agtttcacca agattgctta cagtaatagt ggggttttca atccgcttcc
     1801 tcttcactgg caagacgtac tccccgttca tcttgaataa tacgttgtcc ggccaccata
     1861 gtaaatggtt caacaaaggc ctcaactgaa tcctcctctt tgggttgctg ttttggctgg
     1921 atactcggta actctaaatc ttcgttaaag tggacaatct gtttccaact gcgggagaca
     1981 ttatctccaa acaccatcag taaatcacct ggctgagcca gagtcaaccc ccgatgaatt
     2041 gattcaactt catccggaac cacatcaata cagtcttcag aaacaccatt tttcagcagg
     2101 attttccgga gcatttttgg tatttcgtca taaccccgct ttcggcgatc atcatctgct
     2161 ttacaaataa aatggtcaaa aactccagag acagtttttc caacttcctg aatgtcctca
     2221 tcacgccgat caccgggcat ggaaatcaca caaatatttt ttccaggagt ttccatgcgt
     2281 gataccaggt gacacatacc cttgaaagct gcagggttat gggcataatc caggataact
     2341 ttaaatggat gatcatcaaa aatattcatg cgtcccggag cctggaagaa agtagtgtta
     2401 aaggtacgca atccatgacg gatgtcatcc aatggcactc ccatgctgaa ggagagagct
     2461 gcggcaaaca ttgcattttg aacattatgt actgctttgc cttccaaagt tgccggaatc
     2521 aggtgtgtcc agagcaaagg aatatgagcg cccttatcgt aaagtgtgat catgtctcca
     2581 ttgagcccct tttccagcac taccgccctg ccttcagcac ggatgtgttt acgcaccaga
     2641 ggatgattgt cagctaaagt tacataacaa atattttccg ccttggtatg atttgccatt
     2701 tccagacaaa gtgaatcgtc tgcatttagt actgcagtat cagtggcaat ttcaataata
     2761 atgcgcttta ctcgagcaag ttgttctacg gtatcaatgc ctcttagtcc gaggtgatct
     2821 gcagaaacat tgaggcatgc tgccacatca catttgggga atccaactcc tttcttcagc
     2881 attccaccac gggcaacttc catcactgct acatctaccg atggatcacg gagtaccatt
     2941 tgagaggaaa ctggaccggt catgtcacct tttacagtca ggtgaccatc tatataaacc
     3001 ccatctgtgg aagtcattcc cactgtattc ccgctggtct tcatgatata tgaaagcatc
     3061 ctggcagtag tggtttttcc gttagttcct gtaatagcgg caatcggtat acaactggga
     3121 gatcctggtg gaaacagcat gtccattaca gaggttgcga catcacgcgg tttaccttcg
     3181 ctgggagcta tatgcatacg gaatccagga gcagagttaa cttcaacaat ggcgccgcca
     3241 atatccttgt aagactgggt gatatcgtcc gtcagaaaat caactccacc tacatccaac
     3301 ccaatagatt ttactgctct tacagccatt tctcgattat caggatgcac tacatctgtc
     3361 aaatcaatcg ctgtaccacc agttgataga ttccctgtgg aacgcaggta aaaaatttca
     3421 cccttggtta aaactgtttg actgctgtat tcagattctt tcatcagtct ttccgcctgc
     3481 tcatcaaact tcagacgagt cagtactttt tcgtgaccaa ttccacggcg aggatcacta
     3541 ttgacaattt ccaccagttc ctcaatagtt ttttctccat cgcctactac gtgtcccgga
     3601 acacgtttgg aaacggcaac caactctccg ttgaccacca gcattcgatg atcaaaaccc
     3661 gtaacgtaat tttcgataat gatcgcctcg ttattgcttt gggcacgagc ttcattaaaa
     3721 cccaatacaa cttgctcatc gctaatgaga ttgatcgaga cgccacgtcc atggttgcca
     3781 tcgagtggtt tcacaaccac agggaatcca agattttctg cttgactgac cgcttcctct
     3841 tcactatagg ccagacgctg catggtggtg ggcaggccaa gatcgtacag tatggtgtgg
     3901 gtttcttctt tatcaccggc aatgactaca gcaatctgcc gagtttcact ggtgattgtg
     3961 gcctgtattc ggcgttgata ttttccatga ccaaactgca caaggctaaa ccggttcagt
     4021 cgcaggtatg gaattccacg ctctttggca gcattgacta aagaggctgt actgggtcca
     4081 aattcatgac gctgtgcaaa gcgaatgaaa gcatcgcgct cctcttcaaa attaaaatca
     4141 gcagaagggg gagtatccag ttgttttttg agtgagtccg gaagcagaca cagaagcaac
     4201 ctcagtgcga gttcactggc tttcaagcct acatcccgct gcatgtactg gaacaccatg
     4261 ttatattcgc cgggattgcc tgtggagcgg gtgcggccaa atgttacctc agaccctgca
     4321 atgccctgca gttccaaagc aacatgctcc atgatgtgtc ccatccatgt accatcatct
     4381 tcacgtaatc gacgaatgaa cccaccggga atacgatagg agcatccatg ttctttcagt
     4441 cccggcagta tttcaactaa ctgatcaacg taatcctgcc caatacgcgt ggagggccat
     4501 tcttcaagca ctcctaaatc caaaacgtgt ctaatcaccg ggaaatgggc atacaagctt
     4561 ggaccttgaa aaacattggt tgaaagaatc ttcataaaac ttcacttgaa tctaaagaaa
     4621 atatttggaa gaaattcatc atttaaaaac ctgcctggaa ttgatatcgt attttgcacc
     4681 acttgttaaa agatgcaatc gtaaattggt caggttaatg gcctttcctc tgttttctga
     4741 ttccatagaa gaatgagaca gttcgctggg atcaacaacg gtaattgcac cagaacctac
     4801 aacttccaac ataccttccg gatcaataaa ggctgcagtg ttttcatcaa taccgattcc
     4861 ggttgcaaac ggattgaaag ccactgcaga aagcagccgg cctaaacggt ctcgctgccg
     4921 gaagtgctga tctaccacta tcttgtttgt caatcccagt ccgggagcaa agatcacccc
     4981 gccttcacgt ggagttggcc ctgccttacc ccctgcgatc atatgttccg gaattattgc
     5041 cgcgcccgca gaagttccgg caaagtgaac accaaccgca tttcttcgtc gaattgcctg
     5101 cgcaacggga gtacctccca taatagtgga caaacgtaac tgattgcctc ctgtcacaaa
     5161 aatcccactc gcttcattga gttcttgtag atattctaca cattcgctgt cagagcgttc
     5221 ttcaatcggg agacttacac agtaatcaac ccctatgtcc ctgaaaatat caatgtaatt
     5281 ttggccagtt tctttgagtt ttgaggcagt cggaataatt gccagacgag cgtttttacc
     5341 tccgcatagt tcggcaaact tttgcaaaat aagaggcttt ctccttttat tttcagcccc
     5401 ccctatcggt ataatatagc ccctttgact gccctcagct acgtgcgacg ccacaaatca
     5461 tcctttcttt attaagtaga tcttagctaa gttgaattac ttgccgagct taaattgtaa
     5521 ctaatatacc aaatatacca aaaaaacaga tgaagtaacc agcagtttta ccgaaaatct
     5581 ttccaaagcc agttaataac acaacaaact ttctgctcaa gttgactacc actcctcaat
     5641 tttctacaag gaaaaactca gttcaaaagt ttccgcaact ctttaattga atggaccggc
     5701 aattgacagg tctgattttg gcaaacataa gcggtcgcac gttcatttac cattactctt
     5761 ccgg
//
LOCUS       DTVD01000210            5630 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4708_length_5630_cov_343.734350, whole genome
            shotgun sequence.
ACCESSION   DTVD01000210 DTVD01000000
VERSION     DTVD01000210.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_5630_cov_343.734350"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="EYO68_07170"
     CDS             complement(<1..674)
                     /locus_tag="EYO68_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIC07623.1"
                     /translation="MTLSIVGRCPETGMLGGSITSSSICVASRCLFTRSGVGAALSQN
                     VTDPRLGIRMLDLLESRTGRSGGADVNTVISQVVAEANYMAWRQLVLLDSSGKSAIHT
                     GEKALGRNASAQGNQCAAAANLLANEEVPQTMVTAFEQSSGHLAQRLITALEAGIAAG
                     GEEGPVRSAGVQVVHKVSWPVVDLRVDWDENPLPKLRTIWNEYQPQLEPYVTRALNPE
                     AAPSYGV"
     gene            complement(671..1090)
                     /locus_tag="EYO68_07175"
     CDS             complement(671..1090)
                     /locus_tag="EYO68_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIC07624.1"
                     /translation="MMAHQRIRKFNTKDTYPDQDLDNDLCQVVRAGNTLYLRGQVGTD
                     FDGNLIGFNDPEAQTEQAMKNVKQLLEEAGSCLDHVCKITIYLTSREIREAVYRTVGK
                     WLKGVFPVSTGVIVAGLAKPEWLLEVDVIAVIPEEDK"
     gene            complement(1101..2558)
                     /locus_tag="EYO68_07180"
     CDS             complement(1101..2558)
                     /locus_tag="EYO68_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase"
                     /protein_id="HIC07625.1"
                     /translation="MLRTGNEYRESIRDGREIWIDGERVHDMTTHPAFKPIIDVRARM
                     YDMAHETQYQAQLSYQDAETKELNTTFHKPPRTPEDWQEKDAALDAVMKDIGGVVIRV
                     SDESVGEMWSLYDGQEMLNEVDPQFAKNIEQHILRTTREDPFHVSANTDPKGDRSKTP
                     QEQDPDMLLHVVKETDAGIIVRGAKFETAASYANQAFVKPTIAGWAGNEKLSDYAVSF
                     ICDMGASGLKHICRSGFAGRLPIEDYPLANRFDEVDTLLVFDNVLIPWENVLFYRHTK
                     AAAFIRATLHRYSAYPFVMRLRYVADMIIGAALFNVKQTGLDKNQAVREKLATLACYR
                     EGIHAHLTASIALAEKSPGGLLMPNQSLLYTGRIHACSGLPEMIHLARELCGGQICIT
                     PNHAAFQDTDSGQWLEKYYTVNENWIAEDRRKLLAFARDLLNSDYAGHRLTFQLFAQS
                     PPFAHLNAVYNNFDFSGPLDFVRNAAGLSEKVMKT"
     gene            complement(2593..3561)
                     /locus_tag="EYO68_07185"
     CDS             complement(2593..3561)
                     /locus_tag="EYO68_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07626.1"
                     /translation="MPTKDDLTYPVSLTPPDISAYRAGNIGVEYVHQFDSGKPGPHVM
                     ISAVVHGNELCGAITVDHLLKNEARPLHGKLTMAFMNVEAFLRFDPENPTASRYVDED
                     FNRLWTTEVLDGNRDSVELRRARELRPIVDTVDFLLDIHSMQTVTPPLMMAGPLSKGR
                     RFAEQIGIPETVITDSGHKAGRRMRDYEGFSDPDSPKNALLVECGQHWEKSSSELAIT
                     SAWRFLSILGVILADTAAPHLRILSPTKQHFVDVSGPYTIQTDSFQFVEPFVGLEVIP
                     AAGTIIGHDGDDPVKTPYNNCVLIMPSRRQLRGDSAVRFGRFLDME"
     gene            complement(3740..5236)
                     /locus_tag="EYO68_07190"
     CDS             complement(3740..5236)
                     /locus_tag="EYO68_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HIC07627.1"
                     /translation="MSQTQNADLILRNARIIDGTGGPSQYGDVAIQDDRLLAVGETGN
                     IQASRELDVGGKVVCPGFVDSHTHDDRLLLSDPNMSCKVSQGVTTVVAGNCGISLAPL
                     QIDHRPPPPLDVLVKHQDQFFSGFGTYLQTLAEEPPSLNALCQVGHTTLRAAVMDSYD
                     RPANAEEVSKMRKMLEDSLEAGACGMSTGLFYAPANAAPTSEVIELARALHEHGAFHT
                     THMRDEGEGVADSLRETFTIGAEAEIPVVISHHKCSGAPNHGRSLETLALIDKARKVQ
                     ALGLDAYPYTASSTVLSAYRNFEAKKVLITWSEPMPEYAGRELSEIAKELGMSEKEAV
                     EALNPGGGIFFMMDEEDVQRILAFPQTMIGSDGLPSDTHPHPRLWGTFPRVLGHYVRE
                     VKLFSLEEAVRKMTSLPAACFGLKERGILKPGNYADLVIFDPDTIVDSATFDDPVRPA
                     QGIEQVMVNGRVVWKDGEHTGDRPGRALRLQDLAPFRFDAHPENVSSI"
BASE COUNT         1445 a         1398 c         1305 g         1482 t
ORIGIN      
        1 acaccataag acggagcggc ttccggattg agggcgcggg tgacataggg ctcgagttgc
       61 ggttgatatt cgttccaaat ggtgcgcagt ttgggaagcg gattttcgtc ccaatcaact
      121 cgcaaatcaa caacgggcca cgaaacttta tgaacgacct gcactccggc agaacgcacc
      181 ggcccttctt cgccaccggc ggcaattccg gcttcgagag cggtgataag cctttgtgcg
      241 agatgtccgg agctttgttc aaaagcggtc accattgttt gcggaacttc ttcatttgcc
      301 aaaagatttg cggcggcggc gcactgattt ccttgtgcgg aagcgtttcg gcccaatgcc
      361 ttttctccgg tgtgaatggc tgattttccg gaagaatcca aaagcacgag ttgccgccaa
      421 gccatgtaat ttgcttcggc tacaacttgg gaaatcaccg tgtttacgtc tgctcccccg
      481 gaacgtccgg tgcgagattc cagcaaatcg agcatccgga ttcccaaacg cggatccgtg
      541 acattttgcg acagtgccgc accaactccg gaacgcgtga acaaacaacg actcgccacg
      601 caaatgcttg atgaagtgat tgatccgccg agcatccctg tttccggaca gcgtccgaca
      661 atggaaagcg tcatttgtcc tcctccggta ttacggcaat cacatctact tccagcaacc
      721 attccggttt tgccaaccct gccacaatga ctccggttga aaccggaaac acgcctttga
      781 gccatttgcc gaccgtgcga taaacggctt ctcgaatttc cctgctcgtc aaataaatcg
      841 tgattttaca aacatgatcg agacaacttc cggcttcttc caaaagttgt ttgacatttt
      901 tcatcgcttg ttcggtttga gcttcggggt cattgaaacc aatgagattt ccgtcaaaat
      961 ctgtgccgac ttgtccacgc aaataaagcg tgtttccggc gcgtaccact tgacacaaat
     1021 cattatccaa atcctgatcc ggataagtgt ctttggtgtt gaattttctg attcgttggt
     1081 gtgccatcaa atgttccttt tcaagttttc atcacttttt ccgacaatcc ggcggcatta
     1141 cggacaaaat ccagcggccc ggaaaaatcg aaattgttat agactgcatt cagatgcgca
     1201 aaaggcggag actgcgcaaa caactgaaaa gtcaaacgat gtccggcata atcggaattg
     1261 agtaaatcac gggcaaaagc cagcaatttc cggcggtctt ccgcaatcca gttttcgtta
     1321 acggtgtagt atttttccag ccattgtccg gaatcggtat cttgaaatgc ggcatggttg
     1381 ggcgtgatgc aaatttgtcc gccgcaaagt tcacgcgcta aatgtatcat ttccggaagt
     1441 ccggaacacg cgtgaattcg tccggtgtaa agtagcgatt ggtttggcat cagcaaaccg
     1501 ccggggcttt tttccgccaa cgcaatcgaa gccgtgagat gggcgtgaat tccttcgcgg
     1561 taacaggcga gtgtggccag tttttcgcgg actgcctgat ttttgtcgag tccggtttgt
     1621 ttgacattga aaagtgcggc tccgatgatc atgtccgcaa cataacgcag acgcatcaca
     1681 aaaggataag cactgtaacg gtgaagcgtg gcgcggataa acgccgccgc tttggtgtgg
     1741 cgataaaaaa gcacattttc ccacggaatc aaaacattat caaacaccag caaagtatcg
     1801 acttcatcaa aacggttggc cagcggataa tcttcgatcg gcaagcgtcc ggcgaatccg
     1861 ctccggcaaa tgtgtttgag tccggaagca cccatatcgc aaataaaact gaccgcataa
     1921 tccgaaagtt tttcgtttcc ggcccatccg gcaatggtcg gtttgacaaa agcttgattg
     1981 gcataggacg ccgccgtttc aaattttgca ccgcgcacaa tgatccctgc atcggtttct
     2041 ttgacgacgt gcagtaacat atccgggtct tgctcctgcg gcgttttgga gcggtcgcct
     2101 ttgggatcgg tattggccga aacgtgaaaa gggtcttcgc gggttgtccg gagaatatgc
     2161 tgttcaatgt ttttcgcaaa ctgcggatcg acctcattga gcatttcctg accatcgtaa
     2221 agcgaccaca tttctccaac cgattcatca ctcacgcgaa tcaccacgcc gccgatgtct
     2281 ttcatcacgg catccaaagc cgcatctttt tcctgccaat cttcaggagt tcggggcggt
     2341 ttatgaaaag tggtattgag ttctttggtt tctgcatctt gataactcaa ctgagcttga
     2401 tattgagttt catgagccat atcgtacatc cttgcccgca cgtcaataat cggtttgaaa
     2461 gccggatggg tggtcatgtc gtggacgcgc tctccgtcaa tccagatttc gcgtccgtcc
     2521 ctgatagatt cgcggtattc gtttcctgtg cgtagcatgt tgatccttaa ttttaagtga
     2581 aatagcccta actcattcca tgtctaaaaa ccttccgaaa cgaaccgccg aatccccccg
     2641 caactgtcga cgggaaggca tgatgaggac gcagttattg tacggtgttt ttaccgggtc
     2701 gtccccatcg tgaccgataa tagttcctgc agcaggaatc acctcaagtc cgacaaacgg
     2761 ttcgacaaat tggaacgaat cagtctggat ggtatacggt ccggaaacat caacgaagtg
     2821 ttgtttagtc ggagacaaaa tacgtaaatg aggtgcagct gtgtctgcca aaattacacc
     2881 aagaatacta aggaaacgcc aggcagaggt gattgctaac tcagaactgc ttttttccca
     2941 gtgctgtccg cattcaacaa gcaaagcatt ttttggactg tccggatcag aaaatccttc
     3001 gtagtcacgc atccgacgtc cagctttgtg tccggaatcc gtgataactg tttcaggaat
     3061 accaatctgt tcagcaaatc tccgtccttt agaaagtggc ccggccatca ttaacggtgg
     3121 ggttacggtt tgcatggagt ggatgtccag caaaaaatca acggtatcca caattggacg
     3181 caattcacgc gctcttctta attcgacgct gtcacgattc ccatctaaaa cttctgtagt
     3241 ccaaagtcgg ttaaagtctt catcaacata ccgggacgcg gtgggatttt ctggatcaaa
     3301 tctgaggaat gcctccacat tcataaaagc catagtcagt ttcccatgaa gaggacgcgc
     3361 ttcgtttttc aggagatgat ccacagttat agcaccacat aactcgtttc catgcaccac
     3421 cgcgctgatc atcacatgtg gacccggttt tcctgaatca aattgatgga catattccac
     3481 gccaatattt cctgctcgat aggcggaaat atccggtggt gtcagcgaaa caggatacgt
     3541 gagatcgtct tttgtcggca tgatttctgt tcatggttaa agtagtgaaa cactataaca
     3601 tttcgaatat ttattaccag ttttaagcac tgtcaattcc tgacaactta aaatccagtg
     3661 aatgtaaagt ctttgtgcag gtctacgatt ttatagttcc ctttatttgc tatagaaaag
     3721 gaggagggta cttactgtcc tagattgaag aaacgttttc agggtgcgca tcaaaacgaa
     3781 atggtgccaa atcttgaaga cgcagggccc ggccaggacg atcgccagtg tgttcaccat
     3841 ctttccatac cactcgaccg ttgaccataa cttgttcaat gccctgtgca ggacgaacag
     3901 ggtcatcaaa agttgcactg tccacaatgg tatccggatc aaaaataacc aaatcggcat
     3961 agtttccagg cttaagtatt ccccgttcct tgagaccaaa acaggctgcc ggcagggatg
     4021 tcattttgcg cactgcctct tcgagactga acagcttgac ctcccggaca tagtgtccca
     4081 agacccgtgg gaaagtgccc caaagccgtg gatgagggtg cgtatcggag ggcagaccat
     4141 ccgagccgat catggtttgt ggaaaggcga ggatgcgttg tacgtcttct tcatccatca
     4201 tgaaaaaaat tccacctcca ggattcaggg cttccacggc ctctttctcg ctcattccta
     4261 gttccttggc gatttcagag agctctctcc ctgcatattc cggcatcggc tccgaccaag
     4321 ttatcaacac cttctttgct tcgaaatttc gataggctga aagaacagtc gaactggctg
     4381 tgtagggata agcatcaagt cctaaagctt gaacctttct tgctttatca atcagggcta
     4441 aagtttccaa cgaacggccg tgattggggg caccgctgca tttgtgatgt gaaatcacca
     4501 ctggaatttc tgcttcggct ccaatagtga atgtttcacg caacgaatcg gcaacccctt
     4561 cgccttcgtc ccgcatgtga gtcgtgtgaa aagccccgtg ttcatgcaaa gcgcgggcga
     4621 gttcgataac ctccgaagtt ggcgctgcgt ttgcgggtgc ataaaaaagc ccggtactca
     4681 ttccacaggc tccagcttcc agagaatcct ccagcatttt tcgcatttta cttacttctt
     4741 ctgcatttgc aggacgatca taagaatcca tgacagctgc ccgaagtgta gtgtgaccca
     4801 cctgacaaag agcattgagt gaaggaggtt cttcggctaa tgtctggagg tatgtgccaa
     4861 agccgctaaa gaattgatcc tggtgtttaa ccagcacatc tagcggagga ggaggccgat
     4921 gatctatttg caatggtgcg agacttatcc cgcagtttcc ggcaaccaca gtggttacac
     4981 cctggctaac cttacaactc atgtttggat cagagagtaa caatcgatca tcgtgagtat
     5041 ggctatcaac aaaaccggga caaaccactt ttcctcccac atcaagttca cgacttgcct
     5101 gaatattacc tgtttcaccc actgccagca gacgatcgtc ttggattgcc acgtcgccgt
     5161 attgagaggg cccgcctgta ccatcgatta ttcgggcgtt gcgcagaatg aggtctgcat
     5221 tttgtgtttg ggacatatga tatttgcagt acagtcgttc aaatcatcga aaaaattcag
     5281 gtaactttac aaaaactgct aagttgatta accttcacta ttaagtaagc catgtttttc
     5341 agtgttgctg ccgtttatcc aatgtaacaa acttggtagg attcgcaggc ttaaaatgtc
     5401 cttactatat aaaaataaca gacgaagtca ctagcagttt tactgaaaat ctttccaaag
     5461 ccaaataata acacagcaaa ctttatgcta cagttgaatg acagtcctta attttctaag
     5521 tgaaaaaact caggtcagca gattttgcaa ctctgtaaca gaatggacag gtaactgaca
     5581 agtctgattt tggcaaacat aagcggtcgc acgttcattt accattactc
//
LOCUS       DTVD01000211            5629 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4710_length_5629_cov_365.973986, whole genome
            shotgun sequence.
ACCESSION   DTVD01000211 DTVD01000000
VERSION     DTVD01000211.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_5629_cov_365.973986"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(175..933)
                     /locus_tag="EYO68_07195"
     CDS             complement(175..933)
                     /locus_tag="EYO68_07195"
                     /inference="COORDINATES: protein motif:HMM:PF01464.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07628.1"
                     /translation="MSYCRRYILLRNNGHSQLLLNQNFIIPKINKYHWLLIVFAIILV
                     GCSTKTVGPPGNRQFGNTRIWKQSNPRIKKYVQVYSNNKHVKTCLDRATNSRYLHYIH
                     RVFYKYKLPPELAHLPILESCFDTRADSGSARGMWQFTKSTAKDYGLRVSWLSDDRLN
                     WRKSTHSAARYLKKLGEMFDNNWELALAGYNGGPGYMSRQIKSQGTRNFWELELRKET
                     REYVPKFLAMLRVARKRYPELYFQGSPRAWATSG"
     gene            complement(873..1733)
                     /gene="ispE"
                     /locus_tag="EYO68_07200"
     CDS             complement(873..1733)
                     /gene="ispE"
                     /locus_tag="EYO68_07200"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIC07629.1"
                     /translation="MKILTPAKINPLLYILSKREDGFHELYMHMVPVSLFDTLTFTHN
                     DGQGLNFKVSGAEFSEPPEENLVVRAVRIFELESGETVNLNVILEKHIPSGAGLGGGS
                     GNAAGTIQALNHLYRRSSETEGLLSPETILAIASELGSDVPFFLEPSPCEIKGRGEKI
                     KPLLEFPKLFLIIIKPPLSISTKEAYKKCLPKKQTNLPNVCSIDDLKSYFQNQFETSL
                     LVQYPVLSELKTLLLENGAFGALVSGSGSAVFGAYYNKKLQNQALKKLSCLTVGDIFS
                     CETMVTHSYY"
     gene            complement(1734..3542)
                     /locus_tag="EYO68_07205"
     CDS             complement(1734..3542)
                     /locus_tag="EYO68_07205"
                     /inference="COORDINATES: protein
                     motif:HMM:PF16188.3,HMM:PF16189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC07630.1"
                     /translation="MIEKLREEMIRHELDLLIVVSSDEHLNEYLPVQNWRLKASTVTE
                     SSSGFSGSAGTVVFCIEGRPQLFVDSRYHLQAEQTCSENFDIQKLGNEGVLEPQKWIA
                     AQTQKILKVGADPFVMSPKVWRRYEKALGKSGHTLVPATPNLVDTVWINRPNPPKKEI
                     YPLALQYTGKDSKKKLQEIRKELSESGKSDESKPDVLLLTMLDEIAWLTNMRGSDIEF
                     NPVFEAYAAIFADRAICFCHHPEANLNLHRPDWEFRPYEDYVSFLADLALQKTIKVWI
                     DPSTITMGSRLAFSEDQVFEKESPIVLSKALKNSTEITATKYAHKQAGAGVVRSLQRL
                     QCAFQAGLPVTEKVYANWLYEEYAKAEEFSDLSFSTIAGSGPNGAIVHYGNPDSENPM
                     RSGDLLLVDSGIQCAGGTTDATRTVILGKPDEKQKRIYTLVLQAHIRLARQIFPEGTN
                     GASLDSITRSGLWNAGLDYGHGTGHGVGAFLNVHEGPQRISPVAHDVALQAGMVISNE
                     PGYYENNWGGIRLENLYLVKKADKFPQHPGGKGWLCFEELTLIPFDRTLIDKNLLSAE
                     ELNWLYDYHGRVFSEISPMLDNTEDFQWLTWACGID"
     gene            complement(3795..5387)
                     /gene="pckA"
                     /locus_tag="EYO68_07210"
     CDS             complement(3795..5387)
                     /gene="pckA"
                     /locus_tag="EYO68_07210"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIC07631.1"
                     /translation="MSETSFSLEEYNIHPQTIIRNASPSVLYEEALTYEADATISSDG
                     ALIVSSYEKTGRSPKDKRIVEYPDIMEDVWWGDINMGMDEETFMIARGRAVDYLNTCE
                     RVYVVDGFAGWDPKYRLKIRIVATRPYHALFMHNMLIRPSKEELADYGDPDYVIFNAG
                     RFPANPLTKHMTSRTSVELSFDRKEFVILGTEYAGEMKKGVFTIMNYIMPKQGVLSMH
                     SSANVGKSGDVAIFFGLSGTGKTTLSADPKRQLIGDDEHCWTDDGIFNIEGGCYAKCI
                     NLSEEKEPDIFKAIRFGTVLENVDYDEETHIVDYDGTSHTENTRASYPIDFILNAKIP
                     CVGGHPQNIIFLTCDAFGVLPPVSKLSSSQAMYHFISGYTAKVAGTEMGVTEPEATFS
                     ACFGAAFMVWHPSKYAKLLAERIEKNGTSVWLVNTGWSGGGYGEGERMSLKYTRSIID
                     AILDGSLKNTPSVNNELFGFEVPTECPNVPDEIMQPRRTWSDANAYDQAALKLANMFK
                     ENFKQFEEGSSQEIIQAGPNVS"
BASE COUNT         1556 a         1210 c         1121 g         1742 t
ORIGIN      
        1 cttcttcatc tgccatagca attccttttt aatcttataa aattaataat ttgtgaaaga
       61 agaccaaact ataaaataat ttgatttgaa ctttctcttt caacccattg attgaaatgt
      121 accaactgct gaatccagaa tcaagtcctg ttattctgta aaattagcag cattttatcc
      181 tgatgtagcc catgctcgtg gtgagccctg aaaatatagt tccgggtatc ttttacgtgc
      241 taccctcaac attgccagaa attttggtac atactctcgt gtttctttgc gcagttcaag
      301 ctcccagaaa tttcgtgtgc cttgagattt tatttgtcgt gacatataac ctggtcctcc
      361 gttgtagcca gccaatgcca attcccagtt gttgtcaaac atttcaccca gttttttcaa
      421 ataacgcgcg gctgaatgtg tagattttcg ccaattaagc cggtcatcgg ataaccagct
      481 tacgcgcagg ccataatctt ttgcggttga ttttgtgaat tgccacatgc ctcgtgcact
      541 tccggaatca gctcttgtgt caaaacacga ttccagtatt ggcaaatgtg caagttccgg
      601 aggcagttta tatttataaa aaacccgatg aatgtagtga agatatctgc tgtttgttgc
      661 tctatccaaa caggttttga catgtttatt attgctgtaa acttgaacgt attttttaat
      721 acgcgggttg gattgtttcc agatacgggt attgccgaat tgtctgttac caggggggcc
      781 tacagttttt gtactgcaac ccacaagaat gattgcgaaa acgatcagca gccaatggta
      841 cttatttatt tttggtatta tgaaattctg gttcaataat aactgtgagt gaccattgtt
      901 tcgcaagaga atatatcgcc gacagtaaga catgacaatt ttttaagtgc ctgattttgc
      961 aactttttat tgtaatatgc accaaaaacg gcagagccgc tacctgagac caatgcacca
     1021 aatgcaccat tttcgagcaa taaagtttta agttcagaca agaccggata ttgtacgagt
     1081 aaagatgttt caaactgatt ctgaaagtaa cttttcaaat catcaattga gcatacattt
     1141 ggtaagttag tttgtttttt cggcaggcat tttttgtatg cttccttagt tgaaattgaa
     1201 agaggtggtt ttataatgat gagaaagagt tttggaaatt caagcagtgg ttttatcttt
     1261 tcaccacgcc cctttatttc gcagggcgat ggttcaagaa aaaaaggcac atctgaacca
     1321 agttctgacg caattgcaag aatagtttcc ggagacagta gcccttcagt ttctgaagat
     1381 ctccggtata aatgattcaa agcctgtata gttccagccg catttccact tccgccgcca
     1441 agtcccgcac cggaaggtat atgtttttcc aggattacat ttagatttac agtttccccg
     1501 ctttccagtt caaaaatacg tacggctcga actaccaaat tttcttccgg aggctcagaa
     1561 aactctgctc cactcacctt aaaattcagt ccttgtccat cattatgggt gaaggtcaat
     1621 gtgtcaaata aactgactgg aaccatgtgc atatacaatt catggaaacc gtcttcacgt
     1681 ttacttaaaa tataaagcag cggatttatt tttgcgggag tcaggatttt catttaatca
     1741 attccgcaag cccaagtcag ccactggaag tcttccgtat tatccagcat aggtgatatt
     1801 tcactaaaaa cacgaccatg ataatcatac agccagttta gttcttctgc tgaaagcaaa
     1861 tttttatcaa tcagtgtgcg gtcaaatggg atcagtgtca gttcttcaaa acagagccag
     1921 ccctttccac ctggatgctg aggaaactta tctgcttttt ttactaggta aagattctca
     1981 agacgaattc cgccccagtt gttttcgtaa tatccgggtt cgttggagat caccattcct
     2041 gcttgcagag ccacatcgtg tgcaactggt gagatacgtt gcggtccttc atgtacgttc
     2101 aggaatgcac cgacgccatg tcctgttccg tgtccataat ccaatcctgc attccagaga
     2161 cctgaacggg taatggaatc cagtgaagct ccgtttgttc cctctggaaa aatttgacgg
     2221 gcaagtctga tgtgagcctg cagcaccaat gtgtaaatcc ttttttgctt ctcatccggc
     2281 tttccaagaa ttacagttcg tgttgcatct gtggttccgc cggcacattg aattccggaa
     2341 tctactagca gcaaatctcc acttcgcatt gggttttctg agtccggatt tccgtaatga
     2401 acaatagcac cattcggtcc tgaaccggcg attgtgctga aactgaggtc ggaaaattcc
     2461 tctgcctttg catattcttc ataaagccag tttgcgtata ctttttccgt taccggcagc
     2521 cctgcctgaa acgcacattg caaacgctgt aaacttctga caactcctgc accggcctgt
     2581 ttatgagcat attttgtggc agttatttca gttgagtttt taagtgcttt tgacaaaaca
     2641 atgggacttt ctttttcgaa aacctggtcc tcagaaaatg ctaagcgaga acccatcgtg
     2701 atcgttgagg gatcaatcca gactttgatt gttttctgca gggcaagatc tgctaaaaaa
     2761 ctcacgtaat cttcataagg ccggaactcc cagtctggac ggtgcaggtt taaatttgct
     2821 tccggatgat gacaaaagca aattgcacgg tccgcaaaaa ttgctgcata agcttcaaaa
     2881 accggattga attcaatatc gctcccacgc atattcgtga gccaggcgat ttcatcgagc
     2941 attgtgagca gtaagacatc cggttttgat tcatcagatt ttccggattc actcaattct
     3001 ttccggatct cctgcaactt ctttttactg tcttttccag tatattgcaa agccagtgga
     3061 taaatctctt ttttgggagg atttggacga tttatccata ctgtgtccac caaattggga
     3121 gttgctggaa ccaaagtgtg tccggatttg ccaagggctt tttcatagcg ccgccatact
     3181 tttggactca tcacaaatgg atctgcacca acttttaata ttttttgtgt ttgagctgca
     3241 atccatttct gcggttcaag taccccttca tttccaagtt tttgaatatc aaaattttct
     3301 gaacatgttt gctcagcctg caaatgatag cgggaatcga caaaaagctg aggacgtcct
     3361 tcaatacaaa aaacaactgt tccggcagag ccggaaaatc ccgaagaact ttcagtgact
     3421 gttgaagctt tcaaacgcca attttggacg ggcaggtatt cgtttagatg ttcatcggac
     3481 gaaactacga tcagcaagtc tagttcatgc cggatcattt cttcacgcag tttttctatc
     3541 attctcgaat tatatggtta ggattgtttt gaatatgtat taatcaataa tttcatcaca
     3601 atttatctgc cgttgacaag ttaaatacct gcaaaattat tttcttttcc cgctgaaatg
     3661 acacggtttt ggacttttta cgactgcaac atttttagtc ttaggagatg aataacagaa
     3721 gttggaatgt ttttttacag gaaggcagga cgccggcata aacacggcgc aaagcaggaa
     3781 gagtaatgaa aaactcagga cacgtttggt ccagcctgaa taatttcctg gctggaacct
     3841 tcctcaaatt gtttaaaatt ttccttgaac atatttgcca atttgagggc cgcttgatcg
     3901 taagcgtttg catcagacca ggttcttctg ggctgcatga tttcatccgg aacattcgga
     3961 cattcagttg gaacttcaaa tccgaataat tcattattta cagaaggtgt gtttttcaat
     4021 gatccgtcta aaatagcatc aatgatcgaa cgggtgtatt tgagtgacat acgttcacct
     4081 tctccgtaac ctcctccact ccacccggta tttaccagcc agactgaagt tccatttttt
     4141 tcaatacgtt ctgccaaaag ttttgcgtac ttacttggat gccataccat gaaggcagca
     4201 ccgaaacagg cggagaacgt tgcttctggt tctgtgactc ccatttcagt accggcgact
     4261 tttgcggtat aaccactgat gaagtggtac atagcctgtg aagaactgag tttgctgacc
     4321 ggtggcaata caccaaaagc atcacaagta aggaaaatta tattctgcgg atgcccacct
     4381 acacaaggaa tttttgcgtt caaaataaag tcgatcggat aagaggcacg tgtattttca
     4441 gtgtgagagg ttccatcgta atcgacaatg tgggtttctt cgtcataatc cacattttcc
     4501 agcacagtac caaaacggat tgctttgaaa atatccggct ctttttcttc agaaagattg
     4561 atgcactttg cgtagcagcc gccttcaata ttgaaaattc catcgtcggt ccagcagtgt
     4621 tcatcatccc caataagttg ccgtttcgga tctgcagaaa gggtcgtttt tcctgttccg
     4681 gacagaccga aaaagatagc tacatctcca gattttccca catttgcgga actatgcatt
     4741 gacagaacac cttgttttgg catgatgtaa ttcatgatcg tgaaaacgcc ttttttcatt
     4801 tctccggcat attctgtacc aagtattaca aattccttgc gatcaaaact caattcaaca
     4861 ctggtacgcg acgtcatgtg tttggtgagt ggatttgccg ggaaacgtcc ggcattgaaa
     4921 atcacataat ccggatcacc ataatcggct aactcttcct tactgggacg gatcagcata
     4981 ttgtgcatga acaaagcatg ataaggccga gttgcgacaa ttctgatttt aagacggtat
     5041 tttgggtccc agcctgcaaa gccgtcaacc acataaaccc tttcacaggt gtttaaataa
     5101 tcgacagctc tacctcgtgc aatcataaat gtttcttcgt ccatgcccat gttgatatct
     5161 ccccaccata catcctccat aatatcagga tactcaacga ttcttttatc tttcgggctt
     5221 cttcctgttt tttcatatga agagacaatt aaggctccgt cagatgaaat agtagcatca
     5281 gcttcgtatg tcaaagcctc ttcatataag actgaagggg aggcattgcg aatgatcgtt
     5341 tggggatgaa tattatattc ttccaaagaa aatgaagttt cactcatagt cagttttttg
     5401 tggtggttaa acagtacatc tcaggtttaa ttttgtcaaa gcagcctgaa ccgaggtgtc
     5461 tttctaacaa agacaggatt atattaaagt tcttttacag ggatacttgc aagtcttaac
     5521 tgaaacatta gtaaatcaga tcgtaatccg gtgtatgtat agttctagaa aagtgagcaa
     5581 taggattcta tatggaatcc caattcgaaa caagcaaaag aagtaaaat
//
LOCUS       DTVD01000212           19648 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_475_length_19648_cov_490.291124, whole genome
            shotgun sequence.
ACCESSION   DTVD01000212 DTVD01000000
VERSION     DTVD01000212.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 19648)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19648)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19648
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_19648_cov_490.291124"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="EYO68_07215"
     CDS             complement(<1..833)
                     /locus_tag="EYO68_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIC07632.1"
                     /translation="MLVILHANTEETAEDYKRTWSHLQGLPEIRLQKHKVQGRGQHLT
                     EIYLIGNTTKINLEEIESLPAVERVVRISHDFRILGRHSQETGSIDFEYNGVRFNQQN
                     FHIFAGLCAVDNPGNVERMMQALQDNGQECTRMGAYKPRTNPYSFQGHGKECLPYVFE
                     LAGKYGIKVVAMEVTHEQHIEEIDNCLEKLGQPTGVMLQIGTRNTQNFELLKAVGRQH
                     TYPVLLKRGFGISLNESLNAAEYLASEGNNQVIFCLRGMKTSFNAPHRNMVDFAHLPI
                     V"
     gene            complement(888..1277)
                     /locus_tag="EYO68_07220"
     CDS             complement(888..1277)
                     /locus_tag="EYO68_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HIC07633.1"
                     /translation="MEIKGKIKNISETVQISERFRKREFVVEYASNPDYPQPLQFEMV
                     QDRCELLDSFEVGQEVEIFFDLRGREWTNPQGQVKYFNTLQAWKLVSEQSSVTTSPTT
                     PAPTTSAPVPNEKPGWLENESPDDLPF"
     gene            complement(1305..2474)
                     /locus_tag="EYO68_07225"
     CDS             complement(1305..2474)
                     /locus_tag="EYO68_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIC07634.1"
                     /translation="MTIINKINEFHDEMTAWRRDIHQHPELAYEENRTSDFVATKLEE
                     FGIEIHRGLAKTGVVGTIRNGEGPAIGLRADLDALPLQEKNTFNHASSNPGKMHACGH
                     DGHTAMLLGAAKYLASNKNFKGTVNFIFQPAEEGGGGGDLMVKEGLFEKFPVDSVYGL
                     HNWPGMDPGIFGVGSGPIMAAADMFDLTINGRGGHCAMPDQCIDPIVVASQVVSALQT
                     IPSRNTHPVDSVVISVTQIHAGDAYNVIPDSVRMHGSVRTFIPETQEGMPSSMLRVAE
                     GVCAAFGASCELNYIKGYPATINSVPETEISAKAVVDLVGEENLIRDPTPSMGAEDFS
                     YMLQARPGCYVWLGIGTGKGEGGCMLHSSHYDFNDDVLPSGASYWATLVENELAA"
     gene            complement(2649..3827)
                     /locus_tag="EYO68_07230"
     CDS             complement(2649..3827)
                     /locus_tag="EYO68_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIC07635.1"
                     /translation="MTTLYSGGLVFDGKGNLQENQGVLVDDQKISKVAPCGEFEGFSG
                     DKVDTSSGTLLPGLIDCHVHLVYCGEADPKSRLLNLKPGQIVMNAFENAQKTLRSGVT
                     SIRDLGGRDYLEFAVRDACNSGRQLGPTIKAAGRMICMTGGHGNVFGRIADGPDEVLK
                     AVREQIHAGSDVIKLMATGGVMTPGVNPEDAHYTEEELRVGVDEGHRFHRTCASHAQG
                     AAGILNAVRAGMDSIEHGIFMTQECLEAMLEKGTYLVPTLAAVNNIYINRDNGIPDFI
                     VEKTIRVRERHHQSIKMFYEAGGKIAMGTDAGTPFNVHGDNSQELQFMVDLGISNSDA
                     LSFSTADAADLMGLADRGQISEGYIADLLIVSGNPVEDISVVANQKNHRSVIKNGAIV
                     "
     gene            complement(3976..5202)
                     /locus_tag="EYO68_07235"
     CDS             complement(3976..5202)
                     /locus_tag="EYO68_07235"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07636.1"
                     /translation="MHRVPVLFKITAADFMVRTAYQMGKTPLLPIFAAGLGANATFLG
                     LIVSVSTLTGMLLKPIFGLLSDRWGRWIWLLVGTLLFIGIPFLYQLIHTPEELLFLRL
                     VHGLATAIYGPVTVAWVMEQGQKSGSSADNCAELLGWFSMARNGGYLLGPTIAAALLT
                     VIEPATVFTVIGLMSSCALLPVLLLKKFNSPKIPSHSWRQQTADAFRAGFQTPELWLA
                     GSLECVVYLGFYAAKTFLPLYALESGIPILWVGLFFSVQELTHLLARPFGGRLSDRKG
                     YLPVVSCGMLLAAFSLGLLPLANTPSFLLLLALSFGLAQALIFPATLALFAQQMDVRH
                     TGAGAGLIGSFKNFGKIAGPILGGWLIFWQDYSWMLWSMAALLAVWSCGLLLRAFNKK
                     SLIHQSEYGEAISVHT"
     gene            complement(5204..8497)
                     /locus_tag="EYO68_07240"
     CDS             complement(5204..8497)
                     /locus_tag="EYO68_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIC07637.1"
                     /translation="MTTTLSSKEIQHLNTLRWRCIGPPRGGRVVAVAGDPDNSQVFYF
                     GAAAGGIWKTEDGGTYWENVSDGFLESAAVGALTVAPSDPNVIYAGMGESTIRGDVSF
                     GDGVYKSTDAGKTWKNIGLRKTRHISEIRVHPQNPDRVYVGAFGDAFGSNKEASLTRG
                     VFRSKDGGQKWEQILFRSEKAGAIDLSLDPNNPRIIYASFWEAYRNFWSLNSGGPDSS
                     LYRSTDGGDSWTDISDNPGLPKGTKGKIGVSVSPAQSGRIWAIIEAEEAGLYRSDNGG
                     ESWIKTSGNRDLIHRPWYYCHVFADPKHADTVYITNLKMWKSTDGGTNFSEITTPHGD
                     NHDLWIDPENPQRMVQGNDGGGCVSYNGGKTWSTIYNQFTAQFYRIDTDNQFPYRVYA
                     TQQDNTSISVPSASAYGAITLQECTLPGTGESGFIVVKPDDPDIVYIGAVGSSPGGEG
                     QLQHYNHRTRQLRLINVWPEEQFGWAPKDLKYRFQWTFPIAFSPHDFGTVYACGNHVF
                     RTNDEGHSWEKISPDLTRADESKLGLSGGLTVDSSGAEHYGTISIFVESPHEAGVFWT
                     GSDDGLVHTSRDGGKTWQNITPPDLPEWGYIFCIEVSVHDPDTIYLSATRYKLNDYRP
                     FLYKSIDGGKSWNSINGDYPQSEPYTEISRVIREDPVCPGLLFVGSETGIFMSVNDGK
                     NWQKLQGNFPVVPVYDMKIKQDDLVVGTHGRSFWILDDLTPLRELAGQSSGTETEISG
                     IRFFPPRTTYRRTLNWSVNLYLGDGKNYSPDFGLPGTHYQETTPDGEKRFRHLDMGEN
                     PPEGVIVNYFIESEANLTPEKPLELIVLDANGAEIERFSTKKTDEKSDKSESEESEPS
                     DEKPSLTAKVGFNRFVWNMHYPGPEVKIDKSLEEAGYKPLGRGEAGPGGGPIAPPGSY
                     QVCLKSGENEIRHSFEIIKDPRLNTSPEDFSAQFELWIKIRNRVSDVNSAINRIRRIK
                     LQITELLARENFKNSAEEKSFGEIQKAAEALREKFNTLENELTQNQYETPSDRLRHPT
                     MLKQRMEALVSVVAVADAAPPQQAYSVFEHLSALIDQRLAELSELEKQEVVRLNQQID
                     QAGIRKLQG"
     gene            complement(8584..9537)
                     /locus_tag="EYO68_07245"
     CDS             complement(8584..9537)
                     /locus_tag="EYO68_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07638.1"
                     /translation="MTENTQSTEIAGDERFPEEELETLTQHRSLWGDVWRQFRKHKGA
                     MAGVIVFIFITLAVSLGPLIQGIEPSAINFSERNFAPTLAHPLGTDNIGHDTLAQMLA
                     GGQVSLAVGFSAMLIALLLGTLIGVLAGFIKSLDGPLMRLTDLFLALPILPLLLVIIL
                     LFRDTLRTLYGPEAGIFMLIVFVIGITSWMHTARIVRSDVLGIKEREFVIAAHSIGTR
                     KRRIIFRHILPNILSPIMVSATLGIANAIITESALSFLGLGFPPDFPTWGRLLYDGAN
                     FIQITPARVIWPGLFIALTVLSVNYIGDGLRDALDPRLRGR"
     gene            complement(9530..10543)
                     /locus_tag="EYO68_07250"
     CDS             complement(9530..10543)
                     /locus_tag="EYO68_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011048515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07639.1"
                     /translation="MIHYIFRRTLIAIPTLLVISFIIFAILDLAPNDPTGNLPMTVPP
                     EVRAKIRSSLGLDEPMHIRFGLWLVQFCVNEPLNLLHEGFGISIGDAENRTRILSWAT
                     RSPVVDLIIQRTPQTLWVVGLSYLFGILIAIPIGIVSAYRQYSWFDQVGTFISMVGFS
                     VPTFFTGMVAIIIFSVQLRWFPMIYDTTLVVDSWQNFVSQVKQMIMPVAVLALYNASQ
                     LSRFMRASILDNLNLDYVRTARAKGRSERAVLLIHVLRNSLIPVVTLIALGIPTIFSG
                     AIITEQIFRVNGLGQLLIIAIEGADIPLVQTLTFIFAVLIVFFNLIADVVYGILDPRI
                     RYD"
     gene            complement(10625..12340)
                     /locus_tag="EYO68_07255"
     CDS             complement(10625..12340)
                     /locus_tag="EYO68_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07640.1"
                     /translation="MKYIISTLLTLAIAVGIASPVQAKRGSDGQLNLLYWQAPSTMNP
                     NLSGGTKELEASSVVLEPLGRYDENGNLLPWLAEVIPTVANGGISKDLTTITWKIKKG
                     IKWSDGSALTADDAVFTYEYCSNPDTGCTQSNYWNDIKSVTAVNSLTVKVEFTVPKPF
                     PYAPFVGYNAPILQKAQFDGCQGAKAQECTEQNFHPIGTGPFKVVDFKPNDVIVFEAN
                     PNYRDASKPAFGKLVFKGGGDAVSAARAVLETGEMDYAWNLQVEPEILIKMEQGGRGK
                     VVSAFGTSVERLMVNFTNPDPSLGDMRSEYIGGNNNRNAHPYLSDYNVRRALSLAIDR
                     QVLVDAGYGKAGKISCNVLPAPAIYASSANDECKTPNVAEANRLLDAAGWKRGSDGVR
                     QKGGVRISILYQTSTNSVRQATQAFIKEMWKQIGVETELRNLSASVFFGGDISSPDTY
                     QKFYTDIEMYTNNFSGTDPQTYMSNWTCKEMAQKSNKWGGNNMPRWCNPAYDALSAKM
                     SISSAMKDRIRLTKEMNDMLMQDYAMIPLIHRGNVSAHSNTLSGVRMNPWDSELWNIA
                     DWTRK"
     gene            complement(12324..12521)
                     /locus_tag="EYO68_07260"
     CDS             complement(12324..12521)
                     /locus_tag="EYO68_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07641.1"
                     /translation="MQQLSVKEHFEMLLNAVSLKAGFLPYLVGRLFNGIQNFPECHLR
                     SVDPFLLAIHSHDKVYYEVYN"
     gene            complement(12562..13545)
                     /locus_tag="EYO68_07265"
     CDS             complement(12562..13545)
                     /locus_tag="EYO68_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HIC07642.1"
                     /translation="MTNENNKALLKVDGLVKHFPITRGIFRREVGSVNAVDGISFEIQ
                     EGETLGLVGESGCGKSTAARVILQLIPATAGNVFFKGRDLTSLDKEELRKQRQHMQMI
                     FQDPQDSLNPRMTVGSIISEPLMEHKKLQAEERSQRVEQLLISVGMNPKFTNRYPHEF
                     SGGQRQRIGIARALALSPEFIICDEPIAALDVSIQAQVINLLEDLQEEFGLTYLFISH
                     DLSMIRHIADRVAVMYLGRIVELASSQALYSTPLHPYTQALLSAVPIHDPKLERKRKG
                     TILVGDVPSPANPPSGCHFSTRCPRAEDRCFKDSPEWREVSQGHQVACHLV"
     gene            complement(13542..14537)
                     /locus_tag="EYO68_07270"
     CDS             complement(13542..14537)
                     /locus_tag="EYO68_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07643.1"
                     /translation="MSNQSTILQVENISTRFHTQDGLVHAVNGISFELKKGELLGVVG
                     ESGSGKSVTMMSLLKLLPMPPAEIVSGTANFEGQDLIQLDLGELRKVRGGKIGFIFQD
                     PMTSLNPVLKIGYQLAEPMREHMGMSRKKARQRSAELLDLVGIPMAENRLNDFPHQFS
                     GGMRQRVMIAMALACDPKVLIADEPTTALDVTIQAQILDIVKKLRKELGMAIIWITHD
                     LGVVAGIADRVAVMYGGYIVEEGPVDELYSNPKHPYTQGLLKTLPNLEGKRATRLESI
                     EGQPPNLNKKPTSCPFAPRCTHTMDRCRRENPVLIKRKENHKVACWWNPESLVNE"
     gene            14875..15486
                     /gene="rpsD"
                     /locus_tag="EYO68_07275"
     CDS             14875..15486
                     /gene="rpsD"
                     /locus_tag="EYO68_07275"
                     /inference="COORDINATES: protein motif:HMM:PRK05327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIC07644.1"
                     /translation="MPGKSTIKHKVCRALGINVIGSPSAALTLSRRPNRPGQHGASRQ
                     AKISPFGQQLRETQKLKAFYGISSKQLRRYYDKAARGRIQTNVALVQTLETRLDNMAF
                     RMGFSGTLRAARQMVVHCHITVNGKKVNKPGYQIKPSDIVQLRDKSQKIDRYKDWFNF
                     FEQKLAYVQRDTKKFSGTLVQIPDREEIPIDVEDHLVVEFMAR"
     gene            15613..18297
                     /gene="alaS"
                     /locus_tag="EYO68_07280"
     CDS             15613..18297
                     /gene="alaS"
                     /locus_tag="EYO68_07280"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIC07645.1"
                     /translation="MKSSKQIRQEFISFFEERGHRFVRSAPVVPNDDPTLLFSNSGMA
                     QFKEVFLGTGVRDYNRAVNSQKCIRASGKHNDLEDVGHDNYHHTFFEMLGNWSFGDYF
                     KKEAIEWAWELMIGVWGLPKDKLWATVFEGGDGVAADEEAENFWRKSTGINPDQVLRF
                     DKKDNFWEMGEVGPCGPCSELHIDLGEGTCPHSDKHDCSVNLEGCWRFVELWNLVFIQ
                     YQRFPDGHLEPLSAQHVDTGMGLERICRVLQGVDSNYSTDLFIPILDSISEVTGQTDS
                     GREVSVAYRVIADHLRSLSFAIADGALPSNEGRGYVLRRMLRRATRFGRVLNMHEPFI
                     YKLVPILSEVMGDAFPEINKQQKHVQNVIKAEETSFGLTLDRGLEIFEKMAATAETIS
                     AKKISGENAFKLYDTYGFPVDLTRLMCEERGFGVDEVVFEKLMKEQRTRARESGKFKV
                     TLDKWTEISKGADSKFVGYDTFRTKSKLRRHSLNDQGQWQFILDITPFYAESGGQIGD
                     HGTLEQSGKSAGDKKIWNVVDVQKQGDSIIHFCEAVSPGKGNDAPDDSPMTATVDEKL
                     RLSTTNNHTSAHLMHEALRQVLGEHVTQAGSLVNPDILRFDFTHFEKVSVEQLEEIEN
                     IVNSVIRDNIPTDIFETPFQEAIDSGITALFGEKYGDVVRVVKISDFSEELCGGCHVK
                     ATGQIGQFRVFSEEAIASGVRRIVAVTGRKAELLNQEHGRVARSLRQLMNVPEAQVPE
                     MVEKLAEEKRQLEKELQALRSDSALAGVSELVAKAEEVEGVLVLATEIEVDSADTLRN
                     VGQAVREQLPSGVAWLATTTSGSSDDNEKSTLLCVVSDDLIKRGFKAGEMVNAVAQLA
                     EGRGGGKPNMAQAGIKAPEKLADALAKAPDIVRKKLAN"
     gene            18301..19590
                     /locus_tag="EYO68_07285"
     CDS             18301..19590
                     /locus_tag="EYO68_07285"
                     /inference="COORDINATES: protein motif:HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07646.1"
                     /translation="MRYETFIGKRYLFSPRRDRSISVITWISIGGVALGVVALIVATS
                     VMNGFRTNLRDAVTGSLPHITVFAWDDAMKNYSGLQKRLMEHPEVKGVAPYIFKQTLL
                     TGRKQPKGALLRGIDPEQESEVTKISSFLREEVYGFAPSLEKQALISKKLLHRLSHPN
                     ARINRLKDGIILGAKLARQLEAGVGDTVKLVSSEQRMTPIGDVPRIKKLEVIGIFESG
                     ISGYDEVLAFIDYRLLQKIYRMDDKITGLGVSIRDAGTAPEIAMELSEIGVEYVVSNW
                     ADENKSIFQVMKLEKIGLFLILILIIVVAAFNIISSLIMLVLEKSREIAILKALGAKD
                     SSIRRIFFFQGLVIGSLGTISGVLVGLAICWGIMTFDIIDIPAGVYPGGNRIPVLIDW
                     TDVLITTAASFLICVLVTLYPSTKASRLNPVDPLRYE"
BASE COUNT         5430 a         4501 c         4285 g         5432 t
ORIGIN      
        1 tttacgatag gtaaatgagc aaaatcaacc atgttgcgat gcggcgcatt gaatgaagtt
       61 ttcatgcccc tcaaacagaa aatcacctgg ttgttacctt cgctggccag atattctgcc
      121 gcattgagtg actcgttcaa tgaaattcca aaacctcgtt tgagcaaaac cggataggtg
      181 tgctgccgcc caactgcctt gagcaactca aaattttgtg tattccgtgt accaatttgc
      241 agcataacac cagttggctg acccagtttt tccagacagt tatcaatttc ttctatgtgc
      301 tgctcgtgtg ttacttccat agcaacaact ttaattccat attttcccgc caactcaaaa
      361 acatatggta aacattcctt gccgtgtccc tgaaaagaat agggatttgt acgaggttta
      421 tatgcaccca ttctggtgca ctcctgacca ttgtcctgca acgcctgcat catgcgctca
      481 acatttcccg gattgtcaac tgcacataat ccggcgaaaa tgtgaaagtt ttgctggtta
      541 aaacgtactc cgttgtattc aaaatcgatc gaacctgttt cctgtgagtg ccgacccaaa
      601 attctgaaat catgggaaat ccggactacc cgctccactg ccggaagaga ttcaatttcc
      661 tccaggttga ttttagtagt attgccaatc aaataaattt cagtcagatg ctgacctctg
      721 ccctgtactt tgtgtttttg taaacgaatt tcaggcaaac cctgaaggtg actccacgta
      781 cgtttgtaat cttcagcggt ttcttctgta ttagcatgta atattaccag catttatttg
      841 ttttattatt tgttgtttag cagataaact atggaaagga aatccagtta aaatggcaag
      901 tcatccgggg attcgttttc gagccaaccg ggtttttcat tcggaactgg agcagatgtt
      961 gttggggcag gggtggttgg agaagtagtt acagaacttt gctccgaaac cagcttccat
     1021 gcctggagtg tgttaaaata tttcacttga ccctgagggt tcgtccattc ccgccccctc
     1081 aaatcaaaaa atatctcaac ttcctgacct acttcaaatg aatccaacaa ttcgcagcgg
     1141 tcctgaacca tttcaaattg aagtggttga ggataatccg gattgctagc atattccact
     1201 acaaactcgc gttttcgaaa gcgctccgaa atttgcaccg tctcagatat attttttatt
     1261 tttcctttaa tttccataat tttttagatt tattatattt attgttaagc tgcgagttcg
     1321 ttttcgacca atgttgccca gtaacttgca ccactaggca ggacatcatc gttaaaatcg
     1381 tagtgagaac tgtgcagcat acatcctccc tcaccttttc ctgtaccgat tcccagccag
     1441 acataacaac cgggacgagc ctgaagcata taagaaaaat cttctgctcc catggagggt
     1501 gttggatcgc gaataagatt ttcttctcca acaagatcaa caaccgcctt tgccgaaatt
     1561 tctgtttccg gaactgagtt gatggttgca ggataacctt taatgtagtt tagctcacaa
     1621 cttgcgccga aagcagcaca aacaccttct gcaacccgta acattgacga gggcattccc
     1681 tcttgtgttt ccggaatgaa ggttcgcaca cttccatgca ttcgtacact atccggaatc
     1741 acattgtaag catcaccggc atgaatttgt gtgacgctga tcaccacaga atcaacagga
     1801 tgcgtattgc ggcttggaat ggtttgcagt gcactaacta cctgagaggc aacaacaatc
     1861 ggatcaatgc actgatcagg cattgcacag tggcctcctc ttccgttaat tgttaaatca
     1921 aacatgtcag ctgctgccat gattggtccc gacccaaccc caaaaattcc gggatccatt
     1981 cccggccagt tgtgtaaacc ataaactgaa tccacaggaa atttctcaaa caaaccttct
     2041 ttaaccatca ggtcacctcc gcctccacct tcttctgcag gttgaaagat gaagttaacc
     2101 gtacctttga aatttttatt tgatgctaaa tattttgcag caccgagcaa catcgctgta
     2161 tgtccatcgt gcccgcaggc atgcatttta ccgggattgg aagatgcgtg attaaatgta
     2221 tttttttcct gtaaaggcag tgcatccaag tctgctctaa gcccaatcgc tggtccttca
     2281 ccatttctaa ttgttccaac aacacccgtt tttgccaacc ccctgtgaat ttctattcca
     2341 aactcttcca gcttggtagc cacaaaatca gatgtccggt tttcctcata cgccaattcc
     2401 ggatgttgat ggatatccct tctccatgct gtcatttcat catgaaactc attaatttta
     2461 ttaattattg tcattttcct ccttggacaa tttaattatg aataatggtt tttgtaatag
     2521 tatgaaatcc acgttgtcat ttcggccgta gtgagaaaac ttaatgatta tagtatagta
     2581 gcttttgaaa aatttctcgc ttcgctctaa atgacacgat tttaaaactt tttacaggtt
     2641 tttcatgatt agactattgc gccattttta ataacactgc gatgattttt ctggtttgca
     2701 accacagaaa tatcttccac tggatttccg gaaacaatca gcagatcagc aatatatcct
     2761 tcactaatct gacctctatc agccaaaccc atcaaatcgg cagcgtctgc tgtggaaaaa
     2821 cttaatgcat cagagttaga tattcccaga tcaaccataa actgcagttc ttgactgttg
     2881 tcaccatgta cattaaaagg agttccggca tctgttccca tcgctatttt gcctccggcc
     2941 tcataaaaca tttttatcga ctgatgatgt cgttcccgta cacgaattgt tttttctaca
     3001 ataaaatccg gaattccgtt gtcccgattg atgtaaatat tgttgactgc agctagtgta
     3061 ggaactagat aggtgccttt ttccaacatg gcttccaggc attcctgcgt catgaaaata
     3121 ccgtgttcaa ttgagtccat tccggcacgt accgcattta atattccggc tgcaccctgt
     3181 gcatgactgg cacaggtacg gtgaaaccga tgcccctcat cgactccaac acgcaactcc
     3241 tcttcagtgt aatgcgcatc ttccgggttt actcctggcg tcatcacgcc cccggtagcc
     3301 atcaatttta ttacatctga accggcatga atttgctctc gtactgcttt caatacttca
     3361 tcaggtccat ccgcaatacg gccaaaaaca ttcccgtgtc ctcctgtcat gcaaatcatt
     3421 cggcctgctg ccttgatagt aggtccgagt tgacgtccgg aattacatgc atctctaacg
     3481 gcaaactcca gataatcccg acctcctaaa tccctgattg aagtaactcc gctacgcaag
     3541 gttttttggg cattttcgaa agcgttcatt acaatttgcc ccggttttaa attcaacaaa
     3601 cgtgactttg gatccgcttc cccgcaatag acaagatgga catgacaatc gattaatccc
     3661 ggcagcaaag tcccacttga tgtgtctacc ttgtcgccac taaagccctc aaattctcca
     3721 caaggtgcaa ctttggaaat cttttgatca tcaactaaaa caccttgatt ctcctgcaga
     3781 ttaccttttc cgtcaaagac taatccacct gaatataaag ttgtcattac tattctaatt
     3841 tctgtttttc atttattttc tattaaaaac gcagcttcca tggtacgctt ttcttcatta
     3901 ttatggcaag acacttgtga gcaggatggt gcagattgcg gttcagggaa cttgatttta
     3961 ctttatggag gctgtttacg tatgaactga aatagcttct ccgtactcgc tttgatgaat
     4021 taatgatttt ttattaaatg ctctcagtaa caatccacag ctccaaacag ccagtaaggc
     4081 tgccatgctc cagagcatcc atgaataatc ctgccaaaag atcagccatc ccccaagtat
     4141 aggccctgcg attttaccaa aatttttaaa cgaaccaatc agtcctgcgc ctgctccggt
     4201 gtgacggaca tccatttgtt gtgcgaaaag tgccagggtc gcagggaata taagggcctg
     4261 tgccaggcca aaactaagtg caagcaggag taaaaacgaa ggcgtgtttg ccaacggcaa
     4321 caaacccaaa gaaaaagccg ccagcagcat tccgcaagaa acgactggta aatagccttt
     4381 acggtcactc aaacggcctc caaacggacg tgccaaaaga tgagtcaatt cttgtacaga
     4441 aaagaacagt ccgacccaca aaatcggaat tccgctttcc agtgcataca acggcaagaa
     4501 ggttttagcg gcataaaaac caagataaac gacacattcc agacttcctg ccaaccaaag
     4561 ttccggtgtc tgaaatcccg cccggaaagc atctgcagtt tgttgccgcc aggaatggga
     4621 aggaattttt ggactgttaa attttttgag caaaagcaca ggcagtaaag cgcaggaact
     4681 cattagtcca attaccgtaa agactgtggc aggttcaatt accgtcagta atgcggcagc
     4741 gatcgtagga ccaagcaggt aacccccatt acgtgccata ctgaaccaac ccagaagttc
     4801 tgcgcagttg tcggcagagc ttccggattt ctgtccctgt tccatgaccc aggccacggt
     4861 gaccggtcca taaatggcag tggccaaacc atgtacaagg cgcaaaaaca ataattcttc
     4921 cggagtatgt attagctggt aaaggaacgg aattccgata aacaggagtg ttcccactaa
     4981 aagccaaatc cagcgccccc agcggtcgga caaaaggccg aagattggtt tgagcaacat
     5041 tccggtcaaa gtggaaaccg aaactatcag tcccaaaaag gtagcatttg cacccaaacc
     5101 ggcggcaaag attggcagaa gaggtgtttt acccatttga taggcagtgc gtaccatgaa
     5161 atctgccgca gtaattttga acaataccgg aactctatgc atcctatccc tgcaacttcc
     5221 ggattcctgc ttgatcaatt tgttgattga gccgtaccac ttcttgtttt tcgagctcac
     5281 tcaactctgc gagccgctgg tcaatcagtg cgctcaggtg ttcaaaaacg gaataagcct
     5341 gttgcggagg agcggcatct gccaccgcga ccacgctgac taaagcctcc atgcgttgtt
     5401 taagcattgt tggatgacga agccggtcag acggtgtttc gtattgattt tgcgtcaatt
     5461 cgttttcaag cgtgttgaac ttttcccgca acgcttccgc tgctttttga atttcgccaa
     5521 aacttttttc ttccgcagaa tttttaaaat tttctcgcgc cagcaactcc gtaatttgaa
     5581 gcttaattct ccggatgcga ttgatggcgg aattcacgtc agagactcta ttcctgattt
     5641 taatccacaa ttcaaattgt gctgaaaaat cctccggaga tgtattcagg cgtggatctt
     5701 tgataatttc aaaggaatgc ctgatttcgt tttcacctga tttcaaacaa acctgataac
     5761 ttccaggtgg agcaatcggc ccgcctccgg gtccggcttc acctcgtcct aagggtttgt
     5821 aacccgcttc ttcaagtgat ttatcaattt tgacttcagg accgggatag tgcatattcc
     5881 agacaaaacg attgaaaccc acttttgcag tcaaagatgg tttttcgtcg gaaggctcgc
     5941 tttcttcgct ttcactttta tccgacttct cgtcagtttt tttagtgctg aagcgctcta
     6001 tttcagcacc gtttgcatcg agaacaatca actccaaagg tttttccggt gtaaggttcg
     6061 cttctgattc tataaaataa ttcacaatca cgccttccgg aggattctct cccatatcca
     6121 aatgacggaa acgtttttcg ccgtccggtg tagtttcctg ataatgagta ccaggcaaac
     6181 caaaatccgg gctgtagttt ttgccgtctc ccagataaag attgacgctc cagtttaagg
     6241 tgcggcggta cgtggtgcgt ggcgggaaaa aacggatacc ggagatttct gtttcggttc
     6301 cggaagattg cccggctaat tccctaagtg gtgtcaaatc gtccagtatc cagaatgacc
     6361 gcccgtgtgt tccaaccacc aaatcatcct gcttaatttt catatcgtaa actggaacca
     6421 ccggaaaatt tccctgaagt ttttgccagt tttttccgtc attgacgctc ataaaaattc
     6481 ctgtttcaga accgacaaac aacaatcccg gacaaacagg gtcttcccgg atgactcggc
     6541 tgatttctgt gtaaggttcg ctttgcggat aatctccgtt gatactgttc caactcttgc
     6601 cgccgtcaat cgatttgtaa agaaaagggc ggtaatcgtt taatttgtat cgcgtggcgg
     6661 aaagatagat tgtgtctgga tcgtgtacgg aaacttcgat gcaaaaaata taaccccatt
     6721 ccggaagatc gggcggcgta atgttttgcc aggtttttcc accgtcccgt gaagtgtgga
     6781 caaggccgtc atcactccct gtccagaaaa ctccggcttc atgtggtgac tcaacaaaaa
     6841 tagaaatagt tccataatgt tccgcaccac tgctgtcaac ggtcaaacca ccagaaagac
     6901 ctagtttgct ttcgtctgca cgggtcaaat caggacttat tttttcccaa ctgtggcctt
     6961 catcgtttgt ccggaaaaca tgatttccgc aagcataaac ggttccaaaa tcatggggag
     7021 aaaaagcaat cgggaacgtc cactgaaagc gatatttcaa atctttcggt gcccaaccaa
     7081 attgttcctc gggccaaaca ttgatgagac gtaattgtct ggtacggtga ttgtaatgtt
     7141 gcaattgccc ttcgccgccg ggagagctac cgactgctcc gatataaaca atgtccggat
     7201 catccggttt gaccacaata aagccgcttt ctccagttcc cggcaaagtg cattcctgaa
     7261 gcgtgattgc tccgtatgcg ctggcactcg gaacgctgat gctggtgttg tcctgttgag
     7321 tggcatagac gcgatacggg aattgattgt cggtgtcaat tcggtaaaac tgagcggtaa
     7381 actggttgta tatagtggac catgtttttc cgccgttgta cgaaacacaa cctccaccgt
     7441 catttccctg caccatacgc tgcggatttt cagggtcaat ccaaaggtcg tgattgtctc
     7501 cgtgcggagt ggtaatttcg ctgaaattag taccgccgtc tgtcgatttc cacattttca
     7561 aattggtgat ataaaccgta tcagcgtgtt ttggatctgc gaaaacgtgg cagtaatacc
     7621 agggacggtg aatcaaatcc cggtttccgg aagttttaat ccagctttct ccgccgttgt
     7681 ctgagcggta taatcctgcc tcttctgctt cgataatggc ccagatacgt ccgctttgtg
     7741 ccggtgatac ggacacgccg attttgcctt ttgtgccttt ggggagtcct ggattgtcac
     7801 tgatgtctgt ccaggaatca ccaccgtcgg ttgaacggta caggctgctg tccggcccac
     7861 cgctgttgag gctccagaaa ttgcggtagg cttcccaaaa tgaagcataa ataatgcgtg
     7921 gattatttgg atcgagactg agatcaattg caccggcttt ttcactccga aaaagaattt
     7981 gttcccattt ttggccgccg tctttggagc ggaaaactcc tcgtgtaagg ctcgcttctt
     8041 tgttggagcc gaaggcatca ccgaaagccc ccacataaac cctatccgga ttttgcggat
     8101 gcacccgaat ttcgctgatg tgacgggttt tccggaggcc gatatttttc caggtttttc
     8161 cggcatcggt agatttatag acaccatcgc caaaagaaac atcaccccgg attgtcgatt
     8221 cacccattcc ggcataaatc acatttggat cagaaggagc taccgtcaaa gcaccgaccg
     8281 cagcactctc caaaaagccg tcggaaacat tttcccagta tgttcctccg tcttcagttt
     8341 tccagatacc tcctgccgcc gcaccaaaat aaaagacctg tgaattatcc gggtcacctg
     8401 ccaccgctac cacacgtcct ccgcgagggg gacctataca gcgccaacgg agggtgttca
     8461 gatgttggat ttctttagaa gataaagtcg ttgtcataat agacctggat cataaaattt
     8521 aacaattgtc taattcaata attttttctg caagctgctg tgctgagact agcagggaac
     8581 ttgttatcgt cctcgtaagc ggggatcaag tgcatctctt agcccatcac ctatatagtt
     8641 aacactgagg acggtcagtg cgataaagag tccaggccaa atcacacgtg caggagtgat
     8701 ctgaataaaa tttgctccgt cataaagtaa tctcccccaa gttgggaaat ccggtgggaa
     8761 tccaagtccg agaaaagata aggctgattc tgtaataata gcgttggcta taccaagtgt
     8821 tgcggaaacc atgatcggac tgagaatgtt tgggagaata tggcggaata ttatgcgtct
     8881 tttcctggtc cctatgctgt gtgcagcaat tacaaattcc cgctccttga tgcccaaaac
     8941 atcacttcgc acaattctgg ccgtgtgcat ccaactggtg atgccaatta caaaaacaat
     9001 cagcataaat attccagctt ccggaccata aagtgtgcgc aaagtatcgc ggaatagtaa
     9061 aataattacc agtaaaaggg gtaaaattgg cagtgcgaga aataaatcag tcaagcgcat
     9121 caaagggccg tccaacgatt tgatgaatcc agccaatact ccgatcagtg ttcccaaaag
     9181 cagtgcaatc aacattgctg aaaatcctac cgctaacgaa acttggccgc ctgccagcat
     9241 ttgcgccagt gtatcgtgac caatattgtc cgtacccagt ggatgggcta aagttggggc
     9301 aaaatttctt tcactaaaat tgatggcact gggttcaatt ccttgaataa gcggaccgag
     9361 tgaaacagcg agtgtgataa aaatgaagac aataacaccg gccatggcac ctttgtgttt
     9421 ccggaactgt cgccaaacat ctccccagag agaacggtgc tgagtgagtg tttcaagttc
     9481 ttcttcggga aaacgctcgt ctcccgctat ttctgtggat tgcgtgtttt cagtcatagc
     9541 gaattctagg atcaagaata ccgtaaacca cgtcggcaat caggttgaaa aagacaatca
     9601 ggacggcaaa aataaaagtc agtgtctgca caagaggaat gtccgctcct tcaatggcaa
     9661 tgattaagag ttgacccaga ccgtttacgc ggaatatttg ttcggtaatg attgctccgc
     9721 tgaaaattgt cggaattccc aaagcaatca gggttacaac tggaatcaaa ctgtttcgca
     9781 agacgtgaat cagcagtact gcgcgttctg aacggccttt cgccctcgct gtccgtacat
     9841 aatccaggtt tagattgtcg agaattgatg cccgcatgaa acggctcaat tgagaggcat
     9901 tgtagagcgc aagtactgcc accggcataa tcatttgttt gacttgtgaa acaaagttct
     9961 gccaactatc caccaccaaa gtcgtgtcgt aaatcatggg gaaccacctt aactggacac
    10021 tgaaaattat gattgcgacc ataccggtaa aaaaagtcgg cacagaaaaa cctaccattg
    10081 aaataaatgt tccaacctga tcaaaccagg aatattgtct gtaggcagaa acaataccaa
    10141 tcggaatcgc aatcaggatt ccaaagaggt aagaaagccc aactacccag agtgtttgcg
    10201 gagtgcgttg aataattaga tccaccaccg gactgcgtgt tgcccaggaa agaattcggg
    10261 tacggttttc tgcatcgcca atactgatgc cgaaaccttc atgcagtaaa ttcaagggtt
    10321 cattgacaca aaattgcacc aaccatagac caaagcgaat gtgcattggt tcgtccagtc
    10381 caagagaact ccggatttta gcccttactt ccggaggtac cgtcatcggt agattgcccg
    10441 tggggtcgtt cggagctaaa tcgaggatgg cgaaaatgat gaaactgatt accaataagg
    10501 tcggaatggc aatcaatgtg cggcggaaga tgtaatgaat cacagtttga aagccttgaa
    10561 gatggaaaaa agcggtttgc agaaagaata tctacaaacc gcaaattgct tgctaaaact
    10621 cgaattactt acgagtccag tctgcaatat tccacagttc tgagtcccat ggattcatac
    10681 gaaccccgga aagcgtatta gaatgagctg aaacattacc ccgatggatt agcggaatca
    10741 tcgcataatc ctgcatcaac atatcattca tctctttcgt aagacgaata cgatctttca
    10801 tggctgacga gattgacatt ttagcggaga gtgcatcata agctggattg caccaacgag
    10861 gcatattgtt accgccccac ttgtttgact tttgcgccat ttccttacaa gtccagttgg
    10921 acatgtaggt ttgcggatcc gtcccactga agttgttggt gtacatctct atgtccgtgt
    10981 aaaacttctg gtaagtgtca ggactactga tatctccacc aaagaaaacg gatgcactta
    11041 gattgcgcag ttcagtttct acaccaattt gtttccacat ttccttaatg aaagcctgag
    11101 tagcctgacg tacggagttt gtggaggtct ggtagagaat cgagattcgg actccaccct
    11161 tctgccgtac tccatccgaa ccacgtttcc agccggctgc atccagcagg cggttggctt
    11221 cagcgacgtt gggagtttta cactcatcgt tagccgatga agcataaata gcaggtgccg
    11281 gcagcacatt acaactgatc tttccagcct taccataacc tgcatccacg agtacttggc
    11341 ggtcaatcgc caaagaaagt gcacgcctta cattgtagtc cgacaagtaa ggatgagcat
    11401 tgcggttatt gtttccgccg atgtattcag aacgcatatc accaagtgat ggatcagggt
    11461 tggtaaagtt gaccatcaaa cgttctacag aggtaccgaa agccgaaact actttacctc
    11521 ttcctccctg ttccattttg atcagaattt ccggctcaac ttgcaggttc caggcataat
    11581 ccatttcacc ggtttccaaa actgcacggg cagcagaaac agcatcgccg ccgcctttga
    11641 aaactaactt gccgaaagcc ggcttggaag catcccgata attgggatta gcttcaaaaa
    11701 cgattacatc attcggctta aaatccacca ctttgaaagg gccggttcca atcggatgaa
    11761 aattttgctc ggtgcattcc tgtgcttttg ctccctggca accgtcaaac tgcgcttttt
    11821 gcaaaatggg tgcattgtag cctacaaaag gggcataagg gaaaggcttt ggaacagtga
    11881 actcaacttt tactgtcaga ctgttgacag cagtcactga cttgatatcg ttccaataat
    11941 ttgattgcgt acaacctgtg tcaggattgg agcagtattc ataagtaaaa actgcatcgt
    12001 ctgcggtcaa ggctgatcca tcagaccact tgatgccttt tttaattttc caagtgatgg
    12061 tagtaaggtc ctttgatata cctccgttgg ctactgtagg tattacttct gctagccagg
    12121 gaagcaaatt accgttttca tcatagcgac ctaaaggctc aagaacgacc gaagaagctt
    12181 ccagttcctt ggttccaccg gaaaggttgg ggttcatggt tgatggagct tgccaataga
    12241 gcaagttcaa ttgaccgtca ctgccacgtt tggcctggac gggacttgct atacccaccg
    12301 ctatagcgag ggtcagcagg gtgctaatta tatacttcat agtacacctt atcatgtgag
    12361 tgaatggcca gcagaaaagg atctaccgac cgtaaatggc attcaggaaa attctgaatg
    12421 ccgttgaaga ggcgtcccac taaataaggc aggaagcctg cctttaaact tactgcattc
    12481 agaagcattt caaagtgctc ttttacagaa agttgttgca ttatacagtt cttttataaa
    12541 ttgtcaaaca attttcttca attacactaa atggcaagca acctgatgcc cctgagaaac
    12601 ttctctccat tccggagaat ccttgaaaca acgatcctcg gccctgggac agcgtgtact
    12661 gaagtgacaa cctgaaggcg gattagcagg gcttggtaca tctccaacca ggatagtgcc
    12721 cttgcgtttc ctttccaatt ttggatcgtg gattggaact gctgaaagca aagcctgtgt
    12781 gtagggatga agaggtgtgc tgtaaagggc ctgacttgaa gccagttcaa ctatccgtcc
    12841 aagatacatc acagcaactc gatcggcaat gtggcgtatc atgctcaaat cgtgtgagat
    12901 gaacaaataa gtcaggccaa attcttcctg aagatcctct aaaaggttaa ttacctgtgc
    12961 ttgaattgaa acatccagtg cagcaatcgg ttcatcgcag atgatgaatt ccggactcaa
    13021 ggccagtgca cgggcaatgc ctattcgttg acgttgacca ccggaaaatt catgggggta
    13081 acgattggtg aatttcggat tcatcccgac agaaataagc aattgttcta cacgttgact
    13141 tcgttcttca gcctggagtt ttttatgttc cataagaggc tcactgatga tactacctac
    13201 cgtcatcctt ggattcagac tatcctgtgg atcctgaaaa atcatttgca tatgctgtct
    13261 ttgtttacgt aattcttcct tatcgagaga ggttagatct cgccccttga aaaaaacatt
    13321 tcctgcagta gctggaatta attgaagaat aacccgagcg gcagtagatt ttccgcaccc
    13381 gctttcgcct accaaaccca aggtttcacc ttcctgtatt tcgaaagaaa taccgtcaac
    13441 tgcattaaca cttccgactt ctctgcgaaa tattccacgg gtgatcggaa aatgttttac
    13501 cagtccatca acctttagca aagccttatt attttcattt gtcattcgtt cactaaggat
    13561 tcaggattcc accaacaggc gactttatga ttctcttttc ttttgattaa cacaggattt
    13621 tcccgtcggc aacgatccat ggtatgggta caacgcggtg caaaaggaca acttgttggt
    13681 tttttgttta gatttggagg ttgaccttct atgctttcca agcgtgttgc acgtttacct
    13741 tccagattag gtaacgtttt cagcaagccc tgtgtataag gatgttttgg attactgtat
    13801 agttcgtcca ccggtccttc ctcaactata taccctccgt acattaccgc cacccgatct
    13861 gcaattcctg cgacaacacc aaggtcatgt gtgatccaga taattgccat accgagttct
    13921 ttacgaagct ttttaacgat gtccagtatt tgcgcctgta tggtcacatc aagggccgta
    13981 gttggttcat cggctatcag tactttagga tcacaggcca gggccatggc tatcatgacc
    14041 cgttggcgca ttcctccgga aaactggtgt ggaaaatcat ttaagcggtt ttccgccatt
    14101 ggaataccaa ctaaatccaa aagttccgcc gagcgttgac gagctttctt cctgctcatt
    14161 cccatgtgtt cacgcatcgg ttctgccaat tgataaccaa ttttcaaaac cggattgagt
    14221 gacgtcatcg gatcctgaaa aatgaaacca attttaccac ctcgtacttt cctgagttcg
    14281 ccaagatcaa gctgaatcaa gtcctgtcct tcaaagtttg ccgttccgga aacgatttcc
    14341 gcgggaggca tgggcagaag tttgagcagt gacatcattg tcacgctttt cccggaaccg
    14401 ctttcaccaa ctactccaag cagttctcct ttcttcaact caaaactgat tccattgaca
    14461 gcgtggacca ggccatcctg ggtgtgaaaa cgtgtgctta tattttcgac ttggagaatg
    14521 gtggattgat ttgacaaaat attagaaagc tttttagttt ggagataaaa taatattcaa
    14581 gaaacggagt ctagtcttct ctgatgatat gtcaagaaca atctcgaatt ttcaagagtt
    14641 gaccaagtaa actttataca gaatttcatt ttcaattgac ttaattcatt ttttcataga
    14701 taatcagcag tctatttaat gttctctttg aagtatggag attcagtcgg acccatttca
    14761 tgggatggca tcctgagaat ttttcacaga gagcgaactt aagagaaaat ttttctctaa
    14821 attgttgtta ttctatagtc ctccaaaggt gccatctgaa cgggaaataa atatatgcca
    14881 ggaaaatcta caataaaaca taaggtttgc cgtgcgttgg gaattaatgt aataggaagt
    14941 ccaagtgcag cacttacatt gagtcgtcgt cctaatcgtc caggacagca tggagcaagc
    15001 cgtcaagcaa agatctcccc ttttggtcaa cagttgagag agactcaaaa gctgaaagcc
    15061 ttttacggaa tcagttctaa acagttacgt cgttattacg acaaagcggc aaggggccgt
    15121 atccaaacca acgttgcact cgttcaaact ctcgaaacgc ggttagacaa catggccttt
    15181 cgtatgggtt tttcaggaac attacgtgcc gcccgacaga tggttgttca ctgccacatc
    15241 acagtaaacg gtaaaaaagt caataaacca ggttatcaga taaagcccag tgacattgtt
    15301 caactgagag ataaaagtca aaaaatagac cgatacaagg attggttcaa tttctttgaa
    15361 cagaaattag catacgttca gcgcgacaca aagaaatttt ccggtacgct tgtacagatt
    15421 ccggatcgtg aggaaatacc tatcgacgtt gaggatcacc tggtagtcga attcatggct
    15481 cgctgatttt ttgcattttg gtaccggctg tcccgatgtc agccggttgg atctgatttc
    15541 tttcaaaccc attcccgcct cccgtcctca aacatcggtt caccaaaaaa aacagttttt
    15601 ttaaatcgaa tcatgaaaag ctcaaaacaa attcggcagg aattcatctc tttctttgaa
    15661 gaacgaggac accgttttgt gcgcagtgct ccagtagttc ccaatgacga tccgactttg
    15721 ctcttttcta attcaggaat ggcacagttt aaagaagtgt ttttgggaac cggtgtcaga
    15781 gattacaatc gtgcagtaaa ctctcaaaaa tgtatccgag ccagtgggaa acacaatgac
    15841 ctggaagatg tagggcatga caattaccat cacacttttt ttgagatgct cggcaactgg
    15901 tctttcggag actattttaa aaaagaggcc atcgaatggg catgggaatt aatgatagga
    15961 gtttgggggc ttccaaaaga caaattgtgg gccacagttt ttgagggcgg agacggtgtg
    16021 gctgccgatg aggaagcaga aaacttctgg cggaagtcta ccggaatcaa tccggatcag
    16081 gttttacgtt ttgacaaaaa agataatttc tgggaaatgg gggaagtggg cccctgcgga
    16141 ccatgttctg aactccacat cgatttgggt gaaggtacct gcccgcattc cgacaaacac
    16201 gattgctccg tcaatctcga aggctgctgg cgttttgttg agttgtggaa cctggttttc
    16261 attcaatatc agcgctttcc ggatggccac cttgaaccat tgtcggcaca gcatgtagat
    16321 accggaatgg gtttagagcg aatttgccgt gttcttcaag gagtggattc caattacagt
    16381 acagatcttt ttattccaat tttagacagc atcagcgaag tcaccggaca aacagattcc
    16441 ggcagggaag ttagtgttgc atatcgtgtg attgcagatc atttacgctc gctgagtttt
    16501 gccattgcag atggtgcctt accatccaac gaaggacgcg gatatgtttt gcgtcgaatg
    16561 ttacgcagag caacgcgttt cggacgtgtc ttgaatatgc acgaaccgtt tatttataag
    16621 ctggtgccca ttctgtcaga agtgatgggt gacgcctttc cggaaataaa caagcagcaa
    16681 aaacatgtac aaaatgtaat aaaggctgag gagaccagtt tcggattgac gcttgatcgc
    16741 ggtcttgaaa tatttgaaaa aatggctgca actgcagaaa caatttctgc aaaaaaaata
    16801 tctggagaaa atgcttttaa actttatgac acttacggct tccccgttga tttaacacgg
    16861 ctgatgtgtg aagaacgcgg ttttggagta gatgaagtcg tctttgaaaa attaatgaaa
    16921 gaacagcgca cccgtgcccg tgaatcagga aaatttaagg ttacacttga taaatggaca
    16981 gaaatttcaa aaggagcaga ttctaaattt gtgggctatg ataccttccg gaccaaatca
    17041 aaattgcgga gacattccct caacgatcag ggacaatggc agtttatttt ggacatcaca
    17101 ccattttacg cagaatccgg aggacagatt ggtgatcatg gaaccttgga acaaagcgga
    17161 aaatcagcag gtgacaaaaa aatatggaat gttgtcgacg tgcagaaaca aggagattca
    17221 atcatccact tttgtgaagc tgttagtccc ggaaagggaa acgatgctcc ggatgactct
    17281 ccaatgactg caactgtaga tgaaaaatta cgtttatcta caaccaataa tcacacttct
    17341 gctcacttga tgcacgaagc cttacgtcaa gttttgggtg aacatgttac tcaagccgga
    17401 tcattggtaa atccggatat tttacgtttt gatttcactc attttgaaaa agttagcgta
    17461 gaacaactgg aagaaattga gaatattgtc aactctgtaa tacgtgataa cattccaaca
    17521 gatattttcg aaacaccatt tcaggaagca attgattccg gaatcactgc attgtttgga
    17581 gaaaaatatg gagatgtagt acgtgtggta aaaatatccg atttttcaga agaactttgc
    17641 ggaggctgtc atgttaaagc aactggacaa atcggacagt ttcgcgtttt ttctgaagag
    17701 gcaattgctt ccggagtaag gcggattgtg gctgtaacag gacggaaagc agaattattg
    17761 aaccaggaac atggcagagt tgcacgctct ttgcgccaat tgatgaatgt tcctgaagca
    17821 caggttcctg aaatggtcga aaaacttgca gaagaaaaac ggcaattgga aaaagaactt
    17881 caagctttgc gaagcgatag tgctcttgcc ggagtgtctg aactggtagc aaaagcggaa
    17941 gaagtggaag gagttttagt cctggcaaca gaaattgaag tcgattcggc tgatactctc
    18001 cggaacgtgg ggcaggcagt ccgggaacaa ttaccctctg gcgtggcgtg gctggcaaca
    18061 accacttctg ggagttccga cgacaatgaa aaaagcacct tgctttgtgt agtttccgat
    18121 gatttgatta agagaggatt taaagctggc gaaatggtga atgccgttgc gcaacttgca
    18181 gaaggacgtg gcggagggaa accaaacatg gctcaagcgg gcatcaaagc accagaaaaa
    18241 cttgcagatg cattggcaaa agcaccggat attgtgcgta aaaaactggc aaattaactg
    18301 atgcgctacg aaactttcat aggtaaacgc tatctttttt ctccccgccg tgatcgttca
    18361 atttcagtaa tcacctggat ttcgattgga ggcgtggcat taggagtggt tgccctcatt
    18421 gttgcaacct cagtaatgaa cggtttccgc acaaatttac gggatgccgt aaccggttca
    18481 ttaccgcaca tcactgtctt tgcctgggat gacgcgatga aaaattattc cggactgcaa
    18541 aaacgtttga tggagcatcc tgaagtaaaa ggtgttgcac cctacatttt caagcaaaca
    18601 ctgctaaccg gacgcaaaca gcccaaaggt gctcttctcc gtggaattga tcctgagcag
    18661 gaatcagaag ttacaaaaat ctcctcattc ttacgcgaag aagtttacgg ttttgcccca
    18721 tccctggaaa aacaggcgct tatttcaaaa aaattgcttc atagactttc acaccccaat
    18781 gctaggatta atcgtcttaa agacggaatt attctgggag caaaattggc tcggcaactg
    18841 gaggctggtg taggagatac agtcaagctg gtttcatccg agcaacgcat gactccgatt
    18901 ggcgatgttc cccgaatcaa aaaactggag gtgatcggta ttttcgaatc aggaatttcc
    18961 ggttatgatg aagtgctggc ctttattgat tacaggctgc tgcaaaaaat ttaccgcatg
    19021 gacgacaaaa taacgggtct gggggtgagt atcagagatg cgggaaccgc tccggaaatt
    19081 gctatggaat tgtcagaaat aggtgtggaa tatgttgtca gtaattgggc ggatgaaaac
    19141 aaaagtatct ttcaggtaat gaaactggaa aaaatcggtc tgtttctcat tctcatactg
    19201 atcattgttg tggctgcttt caatatcatc agttcactga ttatgctggt tttggaaaag
    19261 tccagggaaa ttgcaattct caaagctttg ggtgcaaaag attccagtat ccggcgcata
    19321 tttttctttc agggattagt tatcggctcg ctgggaacaa tcagcggagt gttggtaggt
    19381 ttggctatct gctggggaat tatgaccttt gacattattg atattcctgc aggagtttat
    19441 cctggtggaa acaggattcc tgtattgatt gattggacag atgtcctaat aactactgct
    19501 gcttctttct tgatttgtgt acttgtgact ctgtatccat ccacaaaagc ttcccgcctc
    19561 aatccggttg accctctacg ctacgaataa aatattgggc agcatgatgt tttgtaaaaa
    19621 aacttgttat tatgtcgttt atactcca
//
LOCUS       DTVD01000213           19362 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_483_length_19362_cov_352.658828, whole genome
            shotgun sequence.
ACCESSION   DTVD01000213 DTVD01000000
VERSION     DTVD01000213.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 19362)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19362)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19362
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_19362_cov_352.658828"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            90..1742
                     /locus_tag="EYO68_07290"
     CDS             90..1742
                     /locus_tag="EYO68_07290"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HIC07647.1"
                     /translation="MGIMINTFNKVTLRNQQKFPRKIKPRRALLASKRSIKQDRPGKK
                     LSESWLKITHFSLLAVIIFAITFYLTSFLPLSSISSPSLPLKTLHELNLLASKVSSLM
                     QEETVRTEKIEKLLAREYKGVTRLSFIRSENFKLNATVKKKHPELFGQIGNKVSFQIP
                     VGKDGTHGILVISQDISAVGGIFFWIRSLISGSVVLLIVALLVFKLTRRITDQVDVLC
                     RHFVKYRRENESGDLYPTETSDGRTLIGQRIAVLEELWTRFQSMQEELAQNVEELEDS
                     KQKLEQTVTDLQVAKQKERRLVELGNTVAEFGHDIRNANGSISSFANLLLKMLDKDRI
                     KAIEVVQALTYIRRIKNSSNNVTGLTTDILDFAAGRMEIRPEIYQLDEFQEQIESQLG
                     FIDELLLHYRVPAGQPVFLLRIDGRKIIRVIVNLVKNAWEKFQDFPETKDDKPAFIEV
                     RFIPQNMRELKIQIVDNGGPIPDSILTNLFQSYQTEGKEMGTGLGLSISKQLIEAHGG
                     GIRGFNLLDNRGVVFEIILPDCVIPAPSRTVQKQSDSISLSA"
     gene            complement(1795..1992)
                     /locus_tag="EYO68_07295"
     CDS             complement(1795..1992)
                     /locus_tag="EYO68_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07648.1"
                     /translation="MYMQNIDTKLQKKVQSKEFEESVDQAVRSFMYELDFMLLEEQHT
                     TSSLNYSCIQPDAAVKWVNPD"
     gene            2483..3475
                     /locus_tag="EYO68_07300"
     CDS             2483..3475
                     /locus_tag="EYO68_07300"
                     /inference="COORDINATES: protein motif:HMM:PF02628.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HIC07649.1"
                     /translation="MMKWLCIGAALLTWPLIPFGAFVRLKNAGLSCPDWPLCYGQFIP
                     PPGFEIALETGHRFVATLLGILIITITVKTFQQPAYRRHRKLAVISLILVCIQGILGA
                     LTVTMVLWPPVVTMHLLGGNILFGILVYFARVTFLENVEYPSDSSSTHTKPGKDRELK
                     RRHIVLMLAVLFIMIISGGYNSSTSSGSHCEAFPGCHEGSSLSFGMSGTDLSEWTGIE
                     EYILPPAPSDYQGRFLPVYENEWIHMLHRLTAIIGGVVLMIMAWAGLKNRYGHSAIGL
                     SIVALILLEICVGILNAVFRVPAPISATHTAIAATLTGLLFFAAAEGNHNLVKA"
     gene            3472..4005
                     /locus_tag="EYO68_07305"
     CDS             3472..4005
                     /locus_tag="EYO68_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIC07650.1"
                     /translation="MKTLIGNRSAVTLILIASAVILLFLFWLIYFHETPVEGSSLDLT
                     ILPALNALLNLMSATCLIFGFKAIRRGNRVRHKKMMLSALLFSFLFLVSYLTYHAFHG
                     DTLFQGEGWVRPLYFFILISHIILSVVVLPLVLTTVFFAATGNFTMHPKIARITLPLW
                     MYISVTGVLVYLMLYHL"
     gene            complement(4069..4890)
                     /locus_tag="EYO68_07310"
     CDS             complement(4069..4890)
                     /locus_tag="EYO68_07310"
                     /inference="COORDINATES: protein motif:HMM:PF03734.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07651.1"
                     /translation="MVYKFQESWKMHRVFPCTTGKVNGDKFREGDLKTPIGIYWLNQA
                     WAGWELAQYYGNGANVYGTGAFEVSYPNYFDQVIERKNGDGIWIHGTVKGDPIPTRGC
                     ISISNYNFLELTRSVELGATPVIIEEKVTFSPSAVIAQEQQFLMGTIESWKRAWESND
                     VDNYLSFYSSNFLTEKWNFNSWQAHKKSVSTQNKRRRILLNDLSVLKSKNIYHVRFVQ
                     TYTSSSINDVGFKHLFLVKEADGLKIISENWTPLNQLSPSPAFQYAYKESQQNSM"
     gene            complement(5330..5956)
                     /locus_tag="EYO68_07315"
     CDS             complement(5330..5956)
                     /locus_tag="EYO68_07315"
                     /inference="COORDINATES: protein motif:HMM:PF00510.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HIC07652.1"
                     /translation="MTNPSSQSVLFAASEVSLTNRPKRAKPPVSSGVIGMLIFMVTEA
                     MFFAGLISAYMVIRSGIEEWPPWGQPRLPVETTAFNTLVLLLSGLTMGFSRNLLQKQK
                     FQEGRRLLGISLLLGTFFLVAQGYEWVQLLNFGMTVSSSVYGGLFYLIIGAHGFHVVG
                     VLAVLIHAWNRLGASNNPITAEGLLPLQLLWYFVVCVWPVLYVLVYLT"
     gene            complement(5953..6282)
                     /locus_tag="EYO68_07320"
     CDS             complement(5953..6282)
                     /locus_tag="EYO68_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caa(3)-type oxidase subunit IV"
                     /protein_id="HIC07653.1"
                     /translation="MSEAYAHPNYVKIWFWLVVLLLISVAGPMLEIPALTIITAFGIA
                     VIKAFLVAANFMHLKFEKQIISFLLILALCLLGVFFFGLAPDIMMTDGEQWIDCIADK
                     SCVEQRL"
     gene            complement(6313..6894)
                     /locus_tag="EYO68_07325"
     CDS             complement(6313..6894)
                     /locus_tag="EYO68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase subunit III"
                     /protein_id="HIC07654.1"
                     /translation="MSMHGPISTHRSATGVPTGRLGTWWLLASEIFIFGGLIACFILF
                     KLNGTPGFEDASLTSVTAGAVNTFVLLTSSLSIVLAHAAIEKGDQNKSFTYIWYTIGA
                     GLVFLGIKTYEYTGKILHGHTITENLFWSFYYTMTGLHALHVIGGMVIMALISFDIRK
                     GYNYQRVELIGIYWHFVDLIWIFLFPLLYIANF"
     gene            complement(6968..8678)
                     /locus_tag="EYO68_07330"
                     /pseudo
     CDS             complement(6968..8678)
                     /locus_tag="EYO68_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
     gene            complement(8700..9479)
                     /locus_tag="EYO68_07335"
     CDS             complement(8700..9479)
                     /locus_tag="EYO68_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIC07655.1"
                     /translation="MIEYFIQSASSFSGDIDNLFLVITLIIGFWFILVFGALVYFTLK
                     FRRKDGVKAEYITGEKHSETKWTHYPHYAVIAMDVVIIAANIIVWVNIKQTLPPKDNL
                     IRVIGQQWSWSFVDAGPDGVLDTADDITTVNDLHVKVDETYHFELQARDVIHNFSVPV
                     FRLRQDALPGRTIKGWFKPTNTGSFDIQCAEMCGYGHGIMGGAITVHTKESYDETIVQ
                     IKNGTYESHYQKRMGTKTPLPSNTSHTEGHNSFAVLLASIF"
     gene            complement(9683..10588)
                     /gene="cyoE"
                     /locus_tag="EYO68_07340"
     CDS             complement(9683..10588)
                     /gene="cyoE"
                     /locus_tag="EYO68_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HIC07656.1"
                     /translation="MNEKTIVANVYPSLNFSKIRGYITLTKPRLLSSVLLSTVLGYIL
                     PVDSSADVLPLLYLLLGTAMIGSGANTLNQWQEQIPDSKMRRTKNRPLPTGKLSGNEA
                     LAFGIIISVAGFTTLWLSLNILTAALGLLTLFSYILIYTPLKQKSVSNTWFGGITGAL
                     PPVMGWTAARGTLDWEVLPIFALLYFWQLPHFFAIAWMYRDDYRRGGFKMLSLEDPTG
                     KKTSVQMLFYGGLLFISSIAIFIIGQLSLVYLVSAVILGLGFLAVISLFFRKSSNENA
                     RKVFLASIIYLPVLNSIIILDKIIY"
     gene            10857..11720
                     /locus_tag="EYO68_07345"
     CDS             10857..11720
                     /locus_tag="EYO68_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIC07657.1"
                     /translation="MKVRSGLIQMALKGDTSMAPGEITKIMIEAHIPYIEEAGEKGVQ
                     VLCMQEVFTQPYFCPSQDTKWYAAVEKIPEGPTTKLMQEYAKKYNMVIVVPIYEEEMT
                     GVYYNTAAVIDADGKYLGKYRKSHIPQVAGFYEKFFFKPGNIGYPVFETAYCKLGVYI
                     CYDRHFPEGWRALALNGAEYIVNPSATVAGLSQYLWELEQPASAAANGCYIGAINRIG
                     TEEPWNIGTFYGSSYIVNPRGKIEAQLDDKTDGLLIHDMDMDAVREIRNLWQFFRDRR
                     PELYGDLTVLQ"
     gene            11781..13934
                     /gene="preA"
                     /locus_tag="EYO68_07350"
                     /pseudo
     CDS             11781..13934
                     /gene="preA"
                     /locus_tag="EYO68_07350"
                     /inference="COORDINATES: protein motif:HMM:PF14691.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433725.1"
                     /note="too many ambiguous residues; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     assembly_gap    12623..12722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13973..15418
                     /gene="hydA"
                     /locus_tag="EYO68_07355"
     CDS             13973..15418
                     /gene="hydA"
                     /locus_tag="EYO68_07355"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIC07658.1"
                     /translation="MSVIIKGGTVVNADQSFRADVYCDDGIIQAVGPDMDAPLGVDVV
                     DAGGCYVMPGGIDPHTHMQLPFMGTVASEDFFSGTTAGAVGGTTMIIDFVIPGPQEDV
                     LETYHKWRGWAEKSATDYSFHVAITWWDDSVPDAMGTLCRDYGVNSFKHFMAYKGAIM
                     ADDEILVNSFTRARELGALCTVHAESGELVDHFQTKIFESGITGPEGHPQSRPPQVEG
                     EAANRAIRFAQMIDVPLYVVHNSCAHSLNEIARARREGQRVFGEVLAGHLLVDDSVYR
                     NPDFEFAAAHVMSPPFRPKANKEALWRGLQSGDLQTTATDHCCFCADQKAMGKDDFRK
                     IPNGTGGIEDRMHVLWTHGVNTGRLTMSEFVAVTSTNSAKIFNIYPRKGMIAAGSDAD
                     LAIFDPQATHTISVKTHHQNVDFNIFEGMELTGINVATVSQGKVVYKDGDVRTESGVG
                     RYVDRPCFAPYYAAVEKRSSATAPTSVDRSS"
     gene            15418..16203
                     /locus_tag="EYO68_07360"
     CDS             15418..16203
                     /locus_tag="EYO68_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07659.1"
                     /translation="MKESVRVVEARKVDLVFQTSDGPVHALSEIDLEINKGDFISLIG
                     PSGCGKTTLLRLIADLEKPTGGDIQVNEMTPEEARLQRAYGYVFQAPALYPWRTVEGN
                     LKLPLEIMGIDKTEQARRSKEVLEMVDLSGFEKKFPWQLSGGMQQRVSIARALTFNPD
                     LMLMDEPFGALDEIVRDQLNEHFHRLWEETHKTVVFVTHSIPEAAYLSTKIVVMSPRP
                     GKIIDIIESTLPEKRTLDMRDTPEFLALSHRIREGLRAGHSYD"
     gene            16213..17091
                     /locus_tag="EYO68_07365"
     CDS             16213..17091
                     /locus_tag="EYO68_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07660.1"
                     /translation="MNWLRTSSFFSIAAVLAFTTVIWYGAAVYLNSDVLIDKYERKKI
                     DWNFTKLVEDSWAMKRPVMPAPHQIMLDMKKSIFGYKISSKRSLVYHGWVTISSTLVG
                     FAMGAVLGILLAVGIVHVSTLNKSVLPWIIASQTIPILAIAPMVIVVLGAIGVTGLLP
                     KAIISAYLCFFPIAIGMVKGLRSPDPMHLDLMKTYNASKSQTFYKLRWPTSTPFLFAS
                     FKVSIAISLVGAIVGELPAGARAGLGARLLTGSYYGQTVQIWSALFVASIISGSFVFL
                     MTVMESKVQKRMGVQA"
     gene            17088..18206
                     /locus_tag="EYO68_07370"
     CDS             17088..18206
                     /locus_tag="EYO68_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07661.1"
                     /translation="MKTSGMFYWLFIICFAIQFLFSRDWMSLGENTVLFALMPLIGAA
                     AALFRKGVTVLMPGKIVITLVVASGCIATLNSVHSADSGTTSWGIWLYVVTLAGLMWE
                     VMGFWSRVETQLNKKTGIVINLLVPLLFGGMLLYLWEMLTIGFEVPQVLLPAPSLIGV
                     AFANSLEMLWADFQQTFLKAVLAGFFMGCVSGIVVAVLVDKVPFLQRGLLPLGNLASA
                     LPIVGVAPIMVMWFGFDWQSKAAVVVMMTFFPMLVNTLTGLKSTDRMELDLMHSYAAD
                     YWQMLLKVRLPNALPFIFNALKINSTLALIGAIVAEFFGTPIVGMGFRISTEIGRMNV
                     DVVWASIAVAALSGSLFYALLAFLERQFTGWHPSFRVS"
     gene            18262..19254
                     /locus_tag="EYO68_07375"
     CDS             18262..19254
                     /locus_tag="EYO68_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC07662.1"
                     /translation="MSIIKKVVMVLLTVGLAFSTQAAEKVTIQLKWVTQAQFAGYYVA
                     QDKGYYQAEGLDVTIKPGGPDIATPQVIAGGGADVIVDWMPSALASREKGVALVNIAQ
                     IFKKSGMMLTCRKDSGIKSPSDFRGKTLGVWFYGNEYPFLSWMSKLGIPTTGGSNGVK
                     VLKQGFNVDPILQKQAECVSTMTYNEYWQIVDAGLSPSELVVYKYEDQGVSTLEDGLY
                     VLEKNLRKKAFVSKMARFLKASIKGWKYAADHPDEAADIVLENDDTGAQTEKHQRRMM
                     REINKLVGNQPQGIGYLIPADYRRTVDVLMSSDSDPVISKKPKGAWTHSIWNAM"
BASE COUNT         5662 a         3894 c         4293 g         5413 t
ORIGIN      
        1 gatcattaaa taagcaaata ttttgcacat ggatgtgtaa aattataatg atttaggaat
       61 ggaaaccatc tggaacttat tgacttggaa tgggtattat gattaatact tttaacaaag
      121 taacattaag aaaccagcaa aaatttccca ggaaaattaa acctagaaga gctttacttg
      181 catcaaaaag gagcataaag caagacaggc cgggaaaaaa actgagtgaa tcatggctta
      241 aaataactca ttttagtttg cttgccgtaa ttatttttgc aatcactttt tacctgacct
      301 cttttcttcc tctctcttcc ataagttctc cttctctccc cttaaaaaca cttcacgaac
      361 tcaatttatt agcgtcaaaa gtcagcagtt tgatgcagga agaaactgtc agaactgaaa
      421 aaatagagaa attgctggcc agagaatata agggagtcac acgattgagt tttatcaggt
      481 cagaaaattt taaattaaac gctactgtta aaaaaaaaca tcctgaactt ttcggtcaaa
      541 ttgggaataa agtctcattc cagatacctg taggaaaaga tggaactcat ggaatacttg
      601 ttatttctca ggatatttct gcagttgggg ggatcttctt ttggatacgt tcgttaatta
      661 gcggaagtgt cgttttgctt attgttgctt tactggtatt taaactaacc cgtcgaatta
      721 cagaccaggt tgatgttctt tgtcgtcatt ttgttaaata tagacgcgaa aacgaatccg
      781 gagatttata tcctaccgaa acttctgatg gtcgaacatt gattggacag cgtatagcag
      841 ttcttgaaga actttggaca cgtttccagt ccatgcagga agagctggct caaaatgtgg
      901 aagaactgga agactcaaaa cagaaattag aacaaactgt gactgatctg caggtggcta
      961 aacagaagga gcgtcggttg gttgagcttg gtaacactgt ggcagaattt ggacatgaca
     1021 ttcgtaatgc aaatggttcc atttcaagtt ttgccaattt gctcttaaaa atgcttgaca
     1081 aggatcgaat aaaggcgata gaggtggttc aggcattgac ctatattcgc aggataaaaa
     1141 actcttcaaa caacgtgaca ggtttgacaa cagatattct ggattttgct gcaggcagaa
     1201 tggagattcg tccggaaata tatcaattag atgaatttca ggaacaaatt gaatctcaac
     1261 ttggttttat agatgaactt cttttacatt atcgtgttcc agcagggcag ccagtcttcc
     1321 ttctcaggat tgacggtcgt aaaattattc gtgtgattgt gaatcttgta aaaaatgcgt
     1381 gggaaaaatt ccaggatttt ccggaaacaa aagatgataa acctgctttc attgaggttc
     1441 gttttattcc acaaaatatg cgggaactaa aaattcaaat tgttgataac ggcggcccaa
     1501 ttccggattc gattttaact aatcttttcc aatcgtatca aactgaaggc aaggaaatgg
     1561 gaactgggct tggactgtca atatccaagc aacttattga agctcatggt ggaggtattc
     1621 gagggttcaa tctgctggac aatcgcgggg tagtgtttga gattattctt cctgactgtg
     1681 ttattcctgc accatcacgt acggtgcaga aacaaagtga ttccatctcc cttagtgcgt
     1741 aataatcctg aatgcagaat tatcctatgt caggtactgc aatccacagg attctcaatc
     1801 cgggttaacc cacttgactg ctgcatctgg ctgaatgcat gaatagttga ggctagaagt
     1861 ggtatgctgt tcctcaagaa gcatgaagtc taattcgtac atgaacgacc gcactgcctg
     1921 atcaactgat tcttcaaact ctttagactg aacttttttc tgtaattttg tatcaatgtt
     1981 ttgcatgtac attttgcaat ccttccaatc tttgtcataa aaaaggccgc acactttgtt
     2041 cacctacctg atgcatccgg tttgcaagcc aaacttgttg tctcacattc tacatcggca
     2101 aaatctaaaa aatcttgaag aaaaaacgga agaattgtca aatctttgac ttaacttgat
     2161 ctaaacagat tattctttaa cttttatgaa aagagttttc aaccggaatg tgatcagtat
     2221 tcctcatttc tgtattaagc ctcttctgtg gcttgagtat aacggttgag attgtcataa
     2281 ttattttctg ctaatatcag ttaacactac agacacgcgt tttatcgtgt cattccgaac
     2341 gaatgtgaag gaacatttat gatatcagta cattatcaga tctatccctt cggtctagat
     2401 gacacggtgt agtcattaaa atcagtaact tactaaaatt cgcgactcac gagtcagtta
     2461 atcaggggcc tagaattaca ttatgatgaa atggttatgc attggggcgg cacttctcac
     2521 ctggccgctt attccttttg gagcatttgt acgtttgaag aatgcagggc tttcctgtcc
     2581 ggactggccg ctttgctacg gacaatttat tccacctccg ggctttgaga ttgctttgga
     2641 aacgggacat aggtttgttg caactttgtt gggaattttg ataattacaa tcactgttaa
     2701 aacatttcag caacctgcat atcgccgcca tagaaaactt gccgtaatca gtttgatttt
     2761 agtttgtatc cagggaattt tgggggcact gactgtaaca atggtgctct ggccgccggt
     2821 tgttacaatg cacctgctgg gcggaaatat tttgtttgga attttggtat attttgctag
     2881 agttactttt ctggagaatg tggaatatcc ttcagacagt tcttcaactc atactaaacc
     2941 gggaaaagat agagagttaa agagacgcca catagttttg atgctggctg tgctttttat
     3001 tatgattata tccggaggtt ataatagctc tacttcatca ggctctcact gtgaagcatt
     3061 tccgggatgc catgaaggaa gttctttatc ttttggaatg agtggtactg acttatctga
     3121 gtggacagga atcgaagagt acattttgcc tcctgcacca tcagattatc agggacgttt
     3181 tcttcctgta tatgaaaacg aatggataca catgctgcac cgcttgacag ccattattgg
     3241 tggagtagta ttaatgataa tggcctgggc cgggctgaag aacagatatg gacatagtgc
     3301 aataggactg agtattgttg cgctaatttt gctggaaata tgcgtgggga ttcttaatgc
     3361 agtattccgt gtacctgcgc caatatctgc cacgcatact gcaattgcag ccaccctgac
     3421 aggactgttg ttttttgcag ctgcagaagg caatcataat ttggtgaaag catgaaaact
     3481 ttaattggaa atcggagtgc agtaacatta atattaattg cttctgcagt gattttatta
     3541 tttttgttct ggctgatata ctttcatgaa actccagtag agggcagctc tcttgatctt
     3601 acaattctac ctgcactcaa tgcattgctg aacttaatga gcgccacatg tcttattttt
     3661 gggtttaaag ctattagaag aggcaaccgg gtgcgtcata aaaaaatgat gcttagtgca
     3721 ttattatttt cttttttatt tttagtcagc tacctcacgt atcatgcctt tcatggggac
     3781 acgttatttc agggtgaagg ctgggttcgt ccgctgtatt tctttatttt gatttcgcac
     3841 attattcttt ctgtggtagt tttgccgctg gttctaacca cagttttctt tgcggcaacg
     3901 ggtaatttca ccatgcatcc caaaattgcc cggattacac tgccgctctg gatgtatatc
     3961 tcagttacag gtgtactggt ttacctgatg ctgtaccatt tatgatgtaa gcggggaaaa
     4021 attaggagaa acgttcttat gaattatgaa cgttattaaa gtctcaaatc acatggagtt
     4081 ttgttgtgac tccttgtagg catattgaaa tgccgggctg ggggaaagtt gatttaaggg
     4141 agtccagttc tcgctgatga tctttaggcc atctgcctct ttgacaagaa agaggtgttt
     4201 gaaaccaaca tcgttgattg aagaagatgt atatgtctgt acaaagcgaa cgtggtaaat
     4261 attcttactc ttaagaaccg aaagatcatt taaaagaata cggcgtcttt tgttttgggt
     4321 actgacactt tttttgtgtg cctgccaact gttaaaattc catttctctg tgagaaaatt
     4381 tgaggaataa aaagacaggt aattatctac atcatttgat tcccaggctc tcttccacga
     4441 ctcaatagta cccatcaaga attgttgctc ttgagcaatt actgcactgg gtgaaaaagt
     4501 taccttttct tcaataatta ccggagtagc tcccaattca acagaacggg ttaattccag
     4561 aaagttataa ttacttatag aaatacaacc tcgggtcggt ataggatcac cttttactgt
     4621 cccgtgaatc cagatgccgt ccccgttttt ccgttcgatt acctgatcaa aatagtttgg
     4681 ataactgacc tcaaaagcac cggttccgta cacattggcc ccattgccgt agtactgagc
     4741 caattcccat ccagcccaag cctgattaag ccaatatatg ccaatgggag tttttagatc
     4801 tccttcacgg aacttgtctc catttacttt gccggttgta caggggaaaa cacggtgcat
     4861 tttccaggat tcctgaaact tatagaccat caagcgacat gcagatttgt ctataactaa
     4921 aacggttgac ccaggataat cactaaaaat cagaccatcc ggtctcagtt catttgcatg
     4981 aacaaagact gaacaccaca ggaaaaccac ccaaaaaaag aaagtttttt tcacgtgaag
     5041 aataaattat tgtgctgcaa gaatttgatt aataatcagg tttttctatg tttactatac
     5101 agccaataac caattgtgaa gagcattaca acaggaagca aagtcaggaa aatagtcgta
     5161 aagtaaaatg cttccaatgt ttcttcactg gaagagaaac agactgcaca ggcatttgct
     5221 atttccggtg caaagaggag gcctaacagg aatgggaaaa ggagaaaatg ttgcagatgc
     5281 attttcaaat tattcttcta gaggtgtgta ctcaaacttg ctgttagcat caggtcagat
     5341 aaaccagcac atacaaaaca ggccagacac aaacgacaaa ataccacagg agttgtaaag
     5401 gaagtagtcc ttctgcagtt ataggattat tagaagcccc caatcggttc caggcatgga
     5461 tgagcacagc caaaactccg accacgtgaa atccgtgtgc gcctattatt aaatagaaca
     5521 aaccaccgta cacactggac gagactgtca tcccgaaatt aagcaattgc acccactcat
     5581 aaccttgagc caccaaaaag aaagtgccta ataaaaggga tattcccagc aatctgcgtc
     5641 cttcctgaaa tttttgtttt tgcagcaggt tgcgtgaaaa tcccatcgtt aacccactca
     5701 acaaaagaac taaagtattg aatgcagtag tttcaaccgg taatctgggc tgtccccatg
     5761 gaggccactc ttcaatgccg gaacgtatca ccatgtatgc actgatgaga cctgcaaaaa
     5821 acatggcttc ggtaaccata aaaataagca ttccaatcac accacttgac acaggcggct
     5881 tagcccgctt tggacgattg gtcaaagaaa cttctgaggc ggcaaataaa acagactggc
     5941 tggaggggtt tgtcacaagc gctgctctac acaagatttg tctgcaatgc agtctatcca
     6001 ctgttctcca tctgtcatca tgatatccgg tgcaagacca aaaaagaaaa cacctagcag
     6061 gcagagggcc agaatcagta aaaacgaaat gatttgtttt tcaaacttca gatgcataaa
     6121 attagctgca acaaggaatg ccttaatgac tgcaattcca aaggcggtta tgattgtcag
     6181 cgcaggaatt tccaacattg gccctgccac gctgatcagc agtaaaacaa caagccaaaa
     6241 ccatatttta acatagttgg gatgtgcgta agcttcactc atagtttgat ctgtaaagat
     6301 gtgttaataa tattagaaat ttgcaatata caagagagga aacaagaaga tccagataag
     6361 atctacaaag tgccagtaaa ttcctatcag ttccacacgc tgataattgt accctttgcg
     6421 gatatcaaat gaaatgagtg ccatgataac cattccgcca ataacatgca gggcatgtaa
     6481 tccggtcata gtataataaa atgaccagaa taaattttcc gtaattgtat gaccatgcag
     6541 aattttgccg gtgtattcat aagttttaat ccctaaaaat accagacctg caccaattgt
     6601 gtaccagatg taggtgaatg atttattttg atctcctttt tcgattgcag catgtgccag
     6661 tactatactc aaactggaag taagaagtac aaatgtattt acagctcctg cagtcacact
     6721 cgtcagggaa gcatcttcaa acccgggagt tccatttagt ttaaagagga taaagcaggc
     6781 aattaatcca ccaaaaataa aaatttctga agccagcaac caccatgttc ctaagcgtcc
     6841 ggttggcacg cctgtagcac tgcggtgtgt tgaaataggg ccatgcatcg acattttata
     6901 tatctttttg tagttaataa ttaaacgtaa gaagttagat cttccgtttt aatgaaataa
     6961 atcagttcta ttccggtgct acattttgcg gccagaaatc ctcttcccgc cctggtgtac
     7021 tatattcata tgctccccta tatacagtag gaagtttttt gaaattgcca tgcggtggcg
     7081 gggaagaagt agaccattcc agagtgtttg ctctccaagg attgtctggt gccttttcac
     7141 ctttgaacat gctgacgaaa aagttgtaaa cgaaaaccag ctgaaataac agcatgacta
     7201 ccaaactgac tgttgcaaaa atccgaatat tctgtaattc ctgtgtcgct aagtctggaa
     7261 aatgctggta attgtatatg cgccggtgct ggcctgccaa tccaaggaag aatagaggca
     7321 ggaaaataca gttgaaagga ataaatgtac cccagaaatg aattttcccc agagtttcat
     7381 tcatcatgcg tccaaacatt ttcggaaacc aaaaggttaa tgccgcaaac attccaataa
     7441 tagtgatagg gaagaaagta taatggaagt gggccagtac aaaatatgaa tcatggaaat
     7501 atacatccag ggcacttgct cctaaataaa ttccggttac accgccaatc aggaaggacg
     7561 ccaaaaaact caaggcccag agcataggag ttttcaattc aatcgaacca ccatagaggg
     7621 tggcaatata gctgaacatc atttccgcaa gcggaattga aatcaagacg gttgtcacga
     7681 cgaaaacatt agccattcgt ggatcaatgc ctgctataaa ctggtgatgc gcccaaacga
     7741 caaaggatag accaccggtc gcgaatgccg tgtacaatac tgttttatat ccgaacaact
     7801 tttttctgga gaaaactgtg ataatatccg ccactatacc caaggtcggc agcagtacaa
     7861 catatacttc aggatgcccg aaaaaccaga aaagatgttc ccaaaggagt gggtctccgc
     7921 ctcctgaagg caggaaaaaa gttgtaccaa ttctctgatc cataaaaagc attaccgctc
     7981 ctgcgataag aggcccaact gacgccatga acaaaataac tgctattact atgaaccaga
     8041 ctgcaattgg aatgtccatc attttcattc ccggagcacg agcgttcatc gcagtagtga
     8101 caaaattgat gcctcctaat aaaaaggaaa taaactccaa agccacggca agcacccaca
     8161 aagaagaacc ccagtctgtg agattgtatt cggcgacaga cgaaagcggg ggataggcag
     8221 tccatgcacc accgaatccc cccccctttt acgaagaaag agagaatcaa aacaagagcg
     8281 ctgaccagaa aaatttgata tgacaaacgg tttatacgtg ggaaaaccat atcatcacaa
     8341 cctatcatca gtggaattag atagttaccg aagcctgcaa taagtactgg cattgccacc
     8401 cagaaaatca tgatggttcc gtgatttgta accagcatgt tatattcacc gggtccgaca
     8461 agtccatatc ctggaaccag ttctcctgga aaagcaagct gcatccggaa agcataggca
     8521 aaatatccac ctataatagc catgatcata ccggtaaaga tgtactgcat ggcaatgatc
     8581 ttatgatcta aaggcagaat atatttgtgt aaaaaattca gatcttcatg atgttccgca
     8641 tggatgcctg tttcgactgc ggcagctgct tggctcattg tgtcctttac taaaataaac
     8701 taaaatatac ttgctaacag cacagcgaag gaattatgac cttcagtatg cgaggtatta
     8761 gatggtaagg gcgtttttgt tcccattcgt ttctgatagt gactttcata tgtaccgttc
     8821 tttatctgta caatggtttc gtcgtagctt tccttcgtat gtactgtgat tgcaccaccc
     8881 ataattccat ggccatatcc acacatttca gcgcattgta tatcaaaaga accggtattt
     8941 gtcggcttaa accagccttt gattgttctt ccaggcaatg catcctgtct caaacgaaaa
     9001 accggaactg aaaaattatg aatcacgtcc cgtgcctgta attcaaagtg atatgtctca
     9061 tccactttta catgcagatc attaactgta gtaatatcat cagcagtatc caagacccca
     9121 tccgggcctg catcaacaaa ggaccagctc cattgctgcc cgatgaccct gataaggttg
     9181 tctttaggag gaagagtctg ttttatgttt acccaaacta tgatattagc ggcaattatg
     9241 accacatcca tagctatcac tgcatagtga ggataatgcg tccattttgt ttcactgtgc
     9301 ttctcgccgg ttatgtactc ggcctttaca ccgtctttcc tgcgaaattt gagtgtgaaa
     9361 taaacaagtg cgccaaatac aagaataaac caaaatccaa tgatcaaagt aatcacaaga
     9421 aagaggttgt ctatatctcc tgaaaatgag gacgcgcttt gtataaaata ttcaatcatc
     9481 taagttttct ctaaaacatc gttaaccgct tttcagcaaa aaaactcata gaattagttc
     9541 agcaatttta aaggttatca ggaattaaac gcaaaaatgt aggcagccag cataaacaat
     9601 acacatccac tgaaaatcaa ataaaatacc cttggtgccc atgcatccga atcatctttc
     9661 tccataaatc ctccttaaca aattaataaa taatcttatc cagaataata atactgttca
     9721 agaccggcaa gtaaataatg gacgcaagaa aaactttacg tgcattctca ttactgcttt
     9781 tccgaaaaaa taaagaaatt actgctaaaa atcccagccc cagtataact gcacttacca
     9841 gataaacaag acttaactgg ccgataataa atatagcaat gctggaaata aaaagcaaac
     9901 cgccgtagaa cagcatttga acactggttt ttttacctgt tggatcctca agtgaaagca
     9961 ttttgaaacc gcctctgcgg tagtcatcac gatacatcca ggcaattgca aaaaaatgtg
    10021 gaagctgcca aaaataaagc agggcaaaaa ttggcaggac ttcccagtcc aacgtacctc
    10081 tagccgccgt ccagcccatc accggaggta atgcgccagt tattcctcca aaccaggtgt
    10141 tactgactga tttctgtttt agaggtgtat agatcagaat gtaagaaaag agggtcagca
    10201 atccaagagc agcagtaagt atgtttagac taagccataa tgtagtgaaa cccgcgaccg
    10261 aaataataat gccaaaagcc aaagcttcat tcccacttag ttttcccgtc ggtaaaggac
    10321 gattctttgt ccttctcatc ttcgagtcag gaatttgttc ctgccattga ttaagtgtat
    10381 ttgcacctga gccaatcata gcagtaccta acagaagata tagcagcggc aagacatcag
    10441 ctgaactgtc cactggaaga atataaccca gtactgtgct taacaatacg ctggatagta
    10501 acctcggttt tgttaaggta atataacctc tgatcttgct gaaattgaga gatggataaa
    10561 cgttggctac aatagttttt tcattcattt ttttgcagaa tcattcgttt aatggctgtg
    10621 ataatactta aaaaactaac acatcaatta attgttgaca agtaaaaata aaaaaactcg
    10681 gaaagtattt tagactgatt tggagtttaa aacatgaaaa agacttgacg agccttgtat
    10741 caacttatat aattttcagt ctttaagaaa caaatttcat tacagcaaag ctcaggaaga
    10801 tgaatgtcgt cctgcagggt tagcttccac attcgatagt tgcaaggaga aaaaaaatga
    10861 aagttcgcag cgggttgatt caaatggccc tcaaagggga tacatccatg gctccaggag
    10921 aaattaccaa aataatgatt gaagcacata tcccttatat tgaggaggca ggagaaaaag
    10981 gggttcaggt gctctgcatg caggaggtct ttacacaacc atatttttgt cccagccagg
    11041 atacaaaatg gtatgcagct gttgaaaaaa taccagaagg accaactacg aaacttatgc
    11101 aggaatatgc aaaaaagtac aacatggtga ttgtcgttcc catctatgaa gaggaaatga
    11161 caggtgtgta ttataacact gcagcagtaa tagatgcaga cggcaaatat cttggtaagt
    11221 accgcaaatc acatattcca caggttgcag gtttttatga gaaatttttc ttcaaacctg
    11281 gaaatatagg ctatcctgtt tttgaaactg cttactgtaa acttggagta tatatttgtt
    11341 acgacaggca tttcccggaa ggttggcggg ctctggcttt gaacggtgca gagtatattg
    11401 ttaatccttc cgccacggtt gcaggactat ctcaatattt atgggaactg gaacagccgg
    11461 cctcagctgc agctaatggc tgctacattg gtgctattaa tcgaattggt accgaagaac
    11521 cctggaatat tggtacgttt tatggctcaa gctacatagt aaatccacga ggtaaaattg
    11581 aagcacagtt ggatgataag actgatggtt tgctgattca tgacatggat atggatgcag
    11641 tcagagaaat acgcaacctc tggcaattct tccgtgaccg tcgtccggag ctttatggag
    11701 atttaactgt actgcaataa ttgtttcaac tggtggtgtt atttgttcaa agattaccgc
    11761 cgaaaacgct gaaaaaaacc atgaccacta tcgacgttca ggccggacgc ttatcgcagg
    11821 aacagctttc cgaaaatttt tccgattctc atccaccttt gaattcgggg aatgcgattg
    11881 ttgaggcaaa ccgctgttat ttttgttatg acgcaccatg tattgatgcg tgtcccaccg
    11941 ctatcgacat accaaatttt atccgcaaaa tttctaccgg caaccttaaa ggatctgcaa
    12001 aagacattct ttcacaaaac attatgggcg gaatgtgtgc ccgtgtttgt ccgacagaaa
    12061 ttctgtgtca aggggcatgt gtccgggaag cacaggagca ccaaccggta cggataggag
    12121 aattgcagcg ctatgctaca gattggtttt ttgagagtgg tgaaacattt tttgaaagag
    12181 gtgaaccgac cggtaaaaga atcgcggttg tcggcggcgg tccggcagga ctttcctgtg
    12241 cacatcaact agcagtctca gggcatgagg ttgtggtctt tgaggcccgc gaaaaattaa
    12301 gcggccttaa tgaatatgga attgcggcct ataaaactgt caacgacttt gcgcagcgtg
    12361 aagttgattt tattttgcag atcggaggta ttgaaaccag aactaatcaa aagttaggtc
    12421 aagaagtaat tctcgaagat cttagaaaag aatacgatgc ggtttttttg agtattggtt
    12481 tgggagatgt aaattcactt ggattggaag gtgaagaacc caaaggtgtt tacgatgcag
    12541 tggaaactat ttctgagttg cgacaatctg taaacttgag agaactgtca gtaggaaggc
    12601 gaattgttgt tatcggcggc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nngctccacc cacagacaaa ttgtacaatg ttgaacgtgc tttccgtgcg ggatggggcg
    12781 gagtggtttg gaaaactttg ggaattgatc ctcccatcgt taatgtcagt tcacgttatg
    12841 gtgcagtgca ctataatggt caacgcatga tgggatttaa caacgttgaa ttgattactg
    12901 accgtccgct tgaagataat cttcaggaca tcaagcaagt caaaaaagac tggcctgatc
    12961 gagcggttgt tgtttcactg atggtaccat gcactgaaaa agactggcag ttcatcatta
    13021 ataaaataga ggacaccgga tgtgacggag tggaactgaa ttttggctgc ccacacggaa
    13081 tgtctgaacg gggaatgggt tctgctgtcg gccaggttcc ggagtacatt gaaatggtca
    13141 cccgctgggt taaggagtgt acagacttac cgtgcctggt caagctgaca ccgaatgtag
    13201 cggatatctg ccatgcggca aacgctgcaa aagccggagg aggtgatgga gtagctttaa
    13261 tcaacactgt caattctatt gtttccgttg atctggattt gatggctccc gaaccgacta
    13321 ttgacggaaa aggctctcac ggcggatatt gcggaccggc agtcaaaccg atagctttgc
    13381 acatggtgac agaaattgcc aaagctccgg aaacgaaaaa tttaccaatc tcagccatcg
    13441 gaggaatatc aaactggcgt gatgccgccg agttcattgc aatgggagcc gccggtgtgc
    13501 aagtttgtac ggcagtcatg cactatggtt ttaaaatagt ggatgacatg attgacggac
    13561 tgaataactg gatggacgaa aagggctatg cgacgttgga ggatttcagg ggcattgctg
    13621 ttccaaatgt gactgagtgg caatttctga acctgaaata cgacatcaaa gcccgcattg
    13681 acgaatcgac ttgcatccat tgtggattat gtcatatttc ttgtgaggat gctgctcacc
    13741 aggctattcg tataataaaa gcaaatggtg atcgacgatt tgaggtcatt gacgaagaat
    13801 gtgttggatg caatctctgc atgtttgcct gtcctgtacc ggactgcatc accatggaac
    13861 gagttgattc aggaactgaa tatcagaact ggacaaccca tccgaataat ccgatgaggg
    13921 tggaagagaa ttaaatctgt agaaatagaa aaactaaata taaggagaaa atatgtcagt
    13981 aattataaaa ggcggaacag ttgtcaacgc agatcaatca ttccgtgcgg atgtttattg
    14041 tgatgacggt attattcagg cggtaggtcc ggatatggat gctcctttgg gagtggatgt
    14101 tgtggatgcc ggaggatgct atgtcatgcc cggcggcatt gacccgcata cccacatgca
    14161 actcccgttc atgggaacag ttgcttcaga agacttcttt tcaggaacaa cagcaggcgc
    14221 ggttggcgga accacaatga ttattgattt tgtgattccg ggaccacagg aagatgtttt
    14281 ggaaacatat cacaagtggc gcggatgggc tgaaaaatcc gctaccgatt acagttttca
    14341 tgtggcaatc acatggtggg acgattcagt gcccgatgca atgggaacac tgtgccgtga
    14401 ctatggagtc aatagtttca aacactttat ggcctacaaa ggcgcaatca tggcagatga
    14461 tgaaatactg gtaaacagtt ttacccgtgc acgagaactg ggcgcacttt gtactgtcca
    14521 cgcggaaagt ggtgagttgg tagatcattt ccagacgaag atttttgaga gcggtatcac
    14581 cgggccggaa ggacatccgc agtcaaggcc gccgcaagta gaaggagagg cggcaaatcg
    14641 tgcaattcgt tttgcgcaaa tgatcgatgt gcctttgtat gtggttcata actcatgtgc
    14701 tcattcactc aacgaaattg cccgtgcccg gcgtgaaggt cagcgtgtat ttggagaagt
    14761 attggctgga catctgctgg ttgatgacag tgtttaccgt aatcctgatt ttgaatttgc
    14821 cgcagcccac gtgatgagtc ctccattccg accaaaagcc aataaagaag ctctttggcg
    14881 cggtttgcag tccggagatt tgcagacaac tgctaccgat cattgctgtt tttgtgcgga
    14941 ccagaaagca atgggaaaag atgacttccg gaaaattcca aatggaaccg gaggtattga
    15001 agatcgaatg catgtgctct ggacacatgg tgtcaatacc ggacgtttaa caatgagtga
    15061 atttgttgca gtaacttcca caaactctgc aaaaatattc aacatatatc cgcgaaaagg
    15121 aatgattgca gcaggtagtg atgcagattt ggcaatattt gatcctcagg ccacacacac
    15181 aatttccgta aaaacgcatc atcaaaatgt ggatttcaat atttttgagg gaatggaatt
    15241 gacagggatc aacgtggcaa ctgtcagtca gggaaaagtg gtttacaaag acggtgatgt
    15301 ccgcacggaa agtggtgtag gccgttatgt ggatcgtcca tgttttgccc catattatgc
    15361 tgcagttgaa aaacggagct ctgctactgc tccaacttcc gtggatcgtt catcgtaatg
    15421 aaagaaagtg tccgggttgt agaggctcgt aaagttgact tggttttcca gacttcagac
    15481 ggcccggtcc acgctctttc tgaaattgat ttggaaatta ataaaggcga ttttatttca
    15541 ctgattgggc cttccggttg tggaaaaacg acgctgttac gtttgattgc agacctggaa
    15601 aaacctaccg gaggcgacat ccaggtgaat gagatgactc cggaagaggc ccgcttgcag
    15661 cgtgcatacg gttatgtttt tcaggcacct gctctttacc catggcgcac tgttgaaggc
    15721 aaccttaaac tgcctttgga aataatgggc atcgacaaaa cggaacaggc ccgacgttcc
    15781 aaagaagttt tggaaatggt cgatctttcc ggttttgaaa aaaagttccc ctggcaactt
    15841 tccggaggca tgcagcaacg cgtttctatc gcccgtgccc ttacatttaa tcctgatttg
    15901 atgttgatgg acgaaccgtt cggtgctctc gatgaaattg tccgtgacca attaaacgaa
    15961 cattttcacc gactttggga agaaacccac aaaactgtag tctttgttac tcactcaatt
    16021 cctgaagcag catacctttc aacaaaaatt gtggttatgt caccgcgtcc cggaaaaatc
    16081 atcgacataa tcgaaagcac ccttccagaa aaacgaactt tagatatgcg ggacactccg
    16141 gaattccttg cactgtcgca ccgcatccgg gaaggtttgc gggcaggtca tagttatgat
    16201 tgaccagatg ttatgaattg gcttcgtacc tcatcttttt tttcgattgc agccgtgctt
    16261 gccttcacca ctgtcatttg gtatggagca gcagtttatc tcaattctga tgttttgata
    16321 gataaatatg aacggaaaaa aattgattgg aattttacca aactggttga agattcatgg
    16381 gccatgaaac gtccggttat gccggcgccg catcaaatta tgctcgacat gaagaagtcg
    16441 atttttggtt acaaaataag ttccaagcgc agcctggttt atcatggatg ggtgacaatt
    16501 tcgtcaaccc tggttggttt tgcaatgggt gcagttttag gtattttgct ggcagtagga
    16561 attgtgcacg tatcaacact caacaaaagt gttttaccct ggataattgc gtcgcagaca
    16621 ataccgattt tggcaattgc accgatggtt atagttgtct tgggagccat tggtgtaaca
    16681 ggtcttttac ctaaagcaat catttctgcc tacctttgtt ttttcccaat tgcaatcggt
    16741 atggtcaagg gcctgcgttc acctgatcca atgcatctgg atttgatgaa aacttacaat
    16801 gcatctaaat ctcaaacatt ttataaatta cgctggccca cctcgactcc ttttctgttt
    16861 gcaagtttca aggtttctat agccatcagt ttggtaggag cgatagtggg agaattaccg
    16921 gctggagcaa gagccggttt gggtgcaaga ttattgaccg gttcttatta cggacagaca
    16981 gtacaaattt ggagtgcatt gtttgtagcc tcgattatct cggggtcatt cgtttttctg
    17041 atgactgtca tggagagcaa agtccaaaaa agaatgggag tacaggcatg aaaacttccg
    17101 gaatgttcta ctggttattt ataatctgtt ttgcaattca gtttctgttt tccagggact
    17161 ggatgtcgct tggagagaat actgtgctgt ttgccctgat gccgctgatc ggagctgcag
    17221 cagccctttt tcgcaaaggg gtaaccgtgc tgatgccggg taaaattgtg ataactctcg
    17281 tggttgcatc cggatgcatt gcgacattaa acagtgtgca ttctgcagat tcgggtacaa
    17341 cttcctgggg catttggctg tatgttgtga cgctggcggg tctgatgtgg gaggtaatgg
    17401 gattctggtc gagggtggaa acccaactca ataaaaaaac tggaattgtc atcaatttac
    17461 ttgttccatt attatttgga gggatgctgc tttatttgtg ggaaatgttg acgataggtt
    17521 tcgaggtacc acaggttttg ctgccggcac cgtccttgat cggagttgct tttgcaaact
    17581 cactggaaat gctctgggct gattttcagc aaacattttt gaaagcagtg ctagccggtt
    17641 ttttcatggg ttgtgtctcc ggaattgtgg ttgccgtttt ggttgataaa gttccttttt
    17701 tacagcgggg cctgcttcca ctggggaatc tggcgagtgc tcttcccatc gttggggtag
    17761 ctccaatcat ggttatgtgg tttgggtttg actggcagtc caaggcagcg gttgtggtta
    17821 tgatgacctt tttcccgatg ctggtgaata ctcttacagg attaaagtcc acggatcgaa
    17881 tggagcttga cttaatgcac tcttatgcag cagactactg gcagatgctt cttaaggtac
    17941 gtcttccgaa cgcactgcct tttattttta acgcattgaa gatcaactct actttggcgc
    18001 ttattggtgc catagtggcg gagttcttcg gtacaccgat tgtcggaatg gggtttcgta
    18061 tttccaccga aattggtcga atgaatgttg acgtcgtatg ggcatccatt gcggtggcag
    18121 cattgtccgg gtcgttgttc tatgccttgc tggctttttt ggaacggcag tttacaggat
    18181 ggcacccatc tttcagggtc agctaacttg cttgttgcaa acaaaaagct gacttaattt
    18241 taatctcgaa tggagagaat tatgagtata ataaaaaaag tagtgatggt gttgctgaca
    18301 gtcgggttgg cattttccac acaagcagca gaaaaagtaa ccattcagtt gaagtgggtc
    18361 acacaggctc agtttgcagg ttactatgta gcccaggaca agggttacta tcaggcagaa
    18421 gggctggatg tgaccatcaa gccgggtgga ccagacattg cgacgcctca ggttatagca
    18481 ggaggaggag ctgatgtgat cgttgattgg atgccttcag cattggcatc tcgtgaaaaa
    18541 ggtgtggctc tggtgaacat tgcccaaatt ttcaaaaagt caggaatgat gctcacttgc
    18601 cgcaaagaca gcgggatcaa atctccttcc gattttcgag ggaaaacgtt gggcgtctgg
    18661 ttttacggaa acgaatatcc tttcctgagc tggatgagca aattgggtat tcctaccacc
    18721 ggcggttcaa acggtgtgaa agtcctcaag cagggcttca acgtggatcc aattctgcaa
    18781 aagcaggcag aatgcgtttc taccatgacc tacaatgaat actggcaaat tgttgatgca
    18841 ggcctcagtc catcagaatt agtggtctac aaatatgaag atcaaggtgt ttctaccttg
    18901 gaagacggtt tgtatgtatt ggaaaaaaat ctgagaaaaa aggctttcgt cagtaaaatg
    18961 gcccgcttcc tcaaagcttc aatcaaaggt tggaagtatg cagcagatca tcctgatgaa
    19021 gcagcagata ttgtcctgga aaatgacgat accggtgcac agacagaaaa acatcagcgt
    19081 cggatgatgc gtgaaataaa caagttggtt ggcaatcagc cccagggcat tggttacctg
    19141 attccagcgg attacaggcg cacagtcgat gttctgatgt catctgattc cgatcctgtg
    19201 atctccaaaa agcctaaagg cgcatggact cactctatct ggaatgctat gtaattccat
    19261 ttcaatttct agttcggaag caacgcagtc ttgtgttgct tccggactct ttcccagcac
    19321 ttgcttaagt tacaatactc tccctttaat atcaactaag ta
//
LOCUS       DTVD01000214            5523 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4852_length_5523_cov_271.831748, whole genome
            shotgun sequence.
ACCESSION   DTVD01000214 DTVD01000000
VERSION     DTVD01000214.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_5523_cov_271.831748"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="EYO68_07380"
     CDS             <1..584
                     /locus_tag="EYO68_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07663.1"
                     /translation="NINSWSDDIFLVKYNSSGTKQWTKKLGIFDNESGVSMTVDSSDN
                     IYVTGYTKEGYDGNSNSENYDIFLMKYNSSGTKQWTKQLGNSAADIGMGVTVDSSDNI
                     YVTGIASGGLDRNTGSVGEDIVLVKYNSSGTKQWTKQLGSSSSDFAWDVTVDSSDNIY
                     VTGFTNGSLEENFNQGSYYDIFLVKYNSDGVKQ"
     gene            1269..2519
                     /locus_tag="EYO68_07385"
     CDS             1269..2519
                     /locus_tag="EYO68_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIC07664.1"
                     /translation="MNHANSSVILIIPSASYRTNPFMDAATKLDLKVLVITDKSQVFS
                     EYYPDNVITMNFEHWQESIENIREWSERYDLKAVIGVDEESIILAAKLSESLCIEHNS
                     LESVKLTKNKYLMRSELKKSGLKSPWFKRFSVHETPKDIFAELSFPCVLKPTFLSASQ
                     GVLRVNSFEEFGKGCEMLSDLLAEEKVKKRGGDQANWILVEEYIPGKEVSIEGIVNDG
                     KLKDLAIFDKPEPLEGPIFPETIFITPTILDEHLQFSLLETAQTALQALGISKGPVHI
                     ELRINDHGNYILECAARSIGGLCSKVLEFKGGMSLEELILRSALGRNVEKTQLIDKAK
                     GVMMMPTEKRGILREIHGIKAALAVKGITDLQTTIKPGEMLEPLPKGDRYLGFLFAEG
                     KDQDTVIIVLQEAWSKIEVVSEKI"
     gene            complement(2581..3180)
                     /locus_tag="EYO68_07390"
     CDS             complement(2581..3180)
                     /locus_tag="EYO68_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07665.1"
                     /translation="MKKLHRSDLFGWSVFDEERNIDFNGTLWVRPQGNVLIDPLPLIE
                     HDKKQLESLGGAAHVIITNSDHIRGTAKVLSLTGAKCWGPLAEKEQFPLDCDGWLQDG
                     DEPISGIEVFSFNGSKTPGELAILIDESTLITGDLIRAHEGGCLCIIPDAKLKNRELA
                     VESIKRMASIKNIQAVIPGDGWPVFNQGHEALLELAGKL"
     gene            complement(3277..4203)
                     /locus_tag="EYO68_07395"
     CDS             complement(3277..4203)
                     /locus_tag="EYO68_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07666.1"
                     /translation="MFKFNDLSDKDEEFNVQDHLLTPRKFFEKRRKAKKVYVFDLRSS
                     EDFETSHLPGAHNLPFENFEDSIYQMPFSGEIMLYGGDEKELFSAAEILYDNGFETFY
                     FIDSYDSLIGGVDESFIDISQKAQEHISNFLNASAEKFKGISIIIETKTDSKANYSIQ
                     FIELSATPVENISIDLEKFQVLVAKEAIPYLEGTEVDLNDKGELEAFNPSMSITEISG
                     SVEEQIQHVLDEEVNPMVASHGGVVSLLEVKEHNAYLEFGGGCQGCGMIDVTLKQGVE
                     VMIKSQIPEIEAIYDVTDHAGGTNPYYQPSAK"
     gene            complement(4395..4601)
                     /locus_tag="EYO68_07400"
     CDS             complement(4395..4601)
                     /locus_tag="EYO68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07667.1"
                     /translation="MNEEILNMQIRKFLKKVGVQSQREIEQAVRRSIENNSLAGIDKL
                     EASVLLEVPEVGIKVSIESEIKLE"
     gene            complement(4671..5060)
                     /gene="iscU"
                     /locus_tag="EYO68_07405"
     CDS             complement(4671..5060)
                     /gene="iscU"
                     /locus_tag="EYO68_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIC07668.1"
                     /translation="MSYSEKVIDHYKEPRNVGSFTKKEEGVGTGLVGAPECGDVMKLQ
                     IKVENNKIVDAKFKTFGCGSAIASSSLATEWVKGKSIDEAMAIQNTEIVKELSLPPVK
                     IHCSVLAEDAIKAAISDYKNRKAVDSQ"
BASE COUNT         1674 a         1089 c         1018 g         1742 t
ORIGIN      
        1 ggaacatcaa ctcgtggagt gatgacatct ttcttgtgaa gtacaattcc tctggaacca
       61 aacagtggac aaaaaagttg ggaatatttg ataatgaatc tggagtttca atgacagttg
      121 actcttcaga caacatctac gtgacgggat atactaaaga aggatacgat gggaactcca
      181 actcagaaaa ttatgacatc tttcttatga agtacaattc ctctggaacc aaacagtgga
      241 caaaacagtt gggaaactct gctgctgaca ttggaatggg agtgacggtt gactcttcag
      301 acaacatcta tgtgacggga attgcaagtg gaggacttga ccggaacacc ggttcagttg
      361 gtgaagatat tgtcctcgtg aagtacaatt cctctggaac taaacagtgg acaaaacaat
      421 tgggatcatc atcatctgat tttgcgtggg atgtgacagt ggactcttct gacaacatct
      481 acgtgaccgg atttaccaat ggaagtcttg aggaaaactt caaccaggga agttattatg
      541 acatctttct tgtgaagtac aattcagacg gtgtcaaaca ataaccattc aaacactaat
      601 actacaagca aggtcattca tatcatctcc tgtgattgac ccccactaat tcttcacagt
      661 cgtacaacac ttcacaacct accatcccca ttcttgaaaa atccaccaaa tgattaaatt
      721 tcttgggttt tcctaaacac ttttcttctg tagtttaggt actattacgg atatactgga
      781 tctggtagga atgtttcaag ttaaggataa tctcgctgat tgcaaagtat gaatttttga
      841 ctactcgaat tatctattta tcatttgttt ttgaggaaaa ggtgaaaaag cttttattca
      901 ttttcctgat gttaagtttt tgtcttgccg tcatctcatg tgatactaaa agtagtacat
      961 caggtcgtat tagtactgac aatacaacca ccactgttcg tgtctggatt ccggaggacg
     1021 gcggttggtt ttaggttgtt ttcagcagaa atggtggatg cgctctgctt atccacccta
     1081 cggttttgtt taataatctc ttggggttta attccccgcc ccttggggcg aaagtatttg
     1141 attcccattc caataccccg ctgtttgctg cggggcagtt tattttggtt tttggcagtt
     1201 cgttgaaggc aaccaatact ttacgcaaac agagccaaag tattttctat cgagacagga
     1261 ttttttagat gaaccatgca aattcttctg taatcctcat cattccttca gcgagttaca
     1321 ggactaatcc attcatggat gcagccacaa aacttgatct taaggtactg gtaattaccg
     1381 acaagtctca ggtattcagt gaatattatc ctgataacgt cataacaatg aattttgaac
     1441 attggcagga gagtatagaa aatattcgtg aatggtctga acgatatgat ttgaaagcag
     1501 tgataggagt tgatgaagaa tccataattt tagcggcaaa acttagcgaa tctctttgca
     1561 tagaacacaa ttcattagaa tctgtaaagc tgactaagaa caaatatctt atgcgtagtg
     1621 aattgaaaaa atccggactg aaatccccgt ggttcaaacg tttttctgtt catgaaacac
     1681 caaaagatat ttttgctgaa ttaagcttcc cttgtgtcct caaaccaaca tttctatccg
     1741 ccagtcaggg tgttcttcgt gttaatagct ttgaggaatt tggaaaaggt tgtgaaatgc
     1801 tgtctgattt gcttgcagaa gagaaagtga aaaaaagagg cggtgatcaa gcaaattgga
     1861 ttctagttga agaatatatt cccggtaaag aagtgtccat cgaaggtata gtcaacgatg
     1921 ggaaattaaa agacttagca attttcgata aaccggagcc gcttgaaggt cctattttcc
     1981 cggaaacaat ttttattact cccacaattt tggatgagca tcttcaattt tccctgttag
     2041 aaacggcaca aacagctctg caggctttgg ggataagcaa agggccggtt catatagaat
     2101 tacgaattaa cgatcatgga aactacatcc tggaatgcgc tgccaggagc attggtggtt
     2161 tgtgttctaa agtccttgaa ttcaaaggag gtatgagctt ggaggaatta atcctgcgtt
     2221 ctgcattagg acggaatgtc gaaaaaactc aactaattga taaagctaag ggcgtcatga
     2281 tgatgccaac tgaaaaaagg ggtattttaa gagaaattca tggtatcaaa gcagctcttg
     2341 ctgtgaaggg aataactgac ttgcaaacaa caatcaaacc cggtgaaatg ctggaaccac
     2401 ttccaaaggg ggatcggtat cttggttttt tgtttgcaga aggaaaagat caggacacgg
     2461 taataatagt ccttcaagaa gcttggtcaa aaattgaagt agtctctgag aaaatctgat
     2521 tttttttata gctgaatatt tttttatgac tgtttctata taaccaaaca gaattataat
     2581 ctagagtttt ccagcaagtt ccaaaagcgc ttcatgtccc tgattaaaga ctggccaacc
     2641 gtctccggga attacagcct gaatgttttt tattgaagcc atccgtttta ttgattcaac
     2701 cgcaagttcc cgatttttca actttgcatc cggaattatg cacagacacc ctccttcatg
     2761 agctctgata agatctccgg taataagagt tgattcatca atcagaatag ccaattctcc
     2821 aggcgtttta gatccgttga agctaaatac ctctattccg cttattggct catctccatc
     2881 ttgcaaccaa ccatcacagt ctagtgggaa ctgttctttt tctgctaaag gaccccaaca
     2941 ttttgcacct gtcaacgata atacttttgc ggtaccgcga atatgatcag agttggttat
     3001 aatcacatgt gccgctcctc ccagactttc caactgcttt ttatcatgtt caatcaacgg
     3061 cagggggtct atcagtacgt ttccctgagg tcgaacccat aaagttccat tgaaatcgat
     3121 attacgttct tcgtcaaaaa cagaccaacc gaagaggtcg gaacgatgaa gttttttcaa
     3181 agtttttcct caattttaaa caaggttaat cagtaaagag cctttctatc ctaattcaac
     3241 aaaacctcat tatttaatat ggaagttaag acaaccttat ttcgctgaag gttggtagta
     3301 cggatttgtt ccgcctgcat ggtcagtaac atcatatatt gcttcaattt caggaatctg
     3361 tgacttgatc attacctcaa caccctgctt taaggtaaca tcaatcattc cgcacccctg
     3421 acagccaccg ccgaactcaa ggtaggcatt atgttctttg acttcaagca gactgacaac
     3481 accgccatga gaagcaacca ttggattaac ttcctcgtca agaacgtgct gaatctgctc
     3541 ttcaacagaa ccactgattt ctgtgatgga catgcttgga ttgaatgctt caagctcacc
     3601 tttgtcattc aaatccactt cggtcccctc cagataagga atggcttctt tagcaaccaa
     3661 aacctggaat ttttctaaat cgatggaaat gttttcaaca ggagtagctg ataattcaat
     3721 gaactgaatg ctgtagtttg ccttgctatc cgttttggtt tctataatta ttgaaatacc
     3781 cttaaatttt tccgcagaag cattgagaaa gtttgaaatg tgttcctgtg ctttctggct
     3841 tatgtcaata aaggattcat ctacaccccc aatcaacgaa tcataagaat cgatgaaata
     3901 aaaggtttca aatccattat cgtaaagtat ctctgcagcc gagaataact ctttctcgtc
     3961 accgccatat agcataattt cacctgaaaa tggcatctgg tagattgagt cttcaaaatt
     4021 ttcgaaaggc agattgtggg ctcccggcag atgagaagtt tcgaaatctt cagagctacg
     4081 caaatcaaag acataaactt ttttggcttt ccttcttttt tcaaaaaact ttctcggagt
     4141 gagaaggtgg tcttgtacat tgaactcttc atccttgtct gataaatcat tgaatttaaa
     4201 cattttaagc ttagcgctaa ggttctaaat aaaaataaca aactagtaac attttttcat
     4261 tcaagttttc gatggattgt acaacaagtt tggcatgtcg actttttgtg agttagttaa
     4321 atgtgagatt attgattaaa aactgctcag caaaaaagat aatttgaaac actttaaatt
     4381 tgtgaaattt agcatcactc cagtttgatt tcactctcta tggaaacttt aattccaact
     4441 tcaggaactt caaggaggac agaagcttcc aacttatcta ttccggcaag tgaattgttc
     4501 tcaatactac gtctaacggc ttgctcaatc tcacgttgtg actgaacccc taccttttta
     4561 agaaatttac gaatctgcat attcaatatt tcttcgttca tttgtatttg tctcccgctt
     4621 atatttattt ttaagctttc tacagtgtta tgagttgatt cacaatttaa tcactgggaa
     4681 tcaacagcct ttcgattttt ataatcactg attgctgctt taattgcgtc ctcagcaaga
     4741 acagaacaat gaatcttaac tggtggaagt gaaagttctt taacaatttc tgtattctga
     4801 atagccattg cttcatctat acttttcccc ttcacccatt ctgtggcaag gctggaactg
     4861 gcaattgcag aaccgcaccc aaatgttttg aactttgcat caactatttt attgttttcc
     4921 accttgattt gaagcttcat aacgtcacca cactcaggtg cgccaacaag cccggtcccc
     4981 acaccttctt cttttttagt gaaacttcca acgtttcttg gttccttata atggtctata
     5041 actttttcgc tgtatgacat gagtctcctt ttgattaata atcttcacaa attctttatt
     5101 gtatgaaagg taaatcccct taatttacca actccattta gattaaattt aaatagtact
     5161 aatttagtac tatattagta gcacaagatt agtaattatg tcaagaacta atttaagaaa
     5221 ttgttatttt atgatgcagt cgtaaaaagc acattaactt aacttactga tattttgaaa
     5281 tataattaat attaatcttg actatttcag agcaatataa cataatatct ctgtattatc
     5341 aatcagataa gaaggattaa ttatgtttca cgcaaaagac aacaaacagg gctacatatt
     5401 tgatccattc gaatatttag gtccaaaacg cctctcagaa ctaaaaaatt catgggccga
     5461 aatttttcgc agtgagatac ttccagaatt gcctgttgag tctcttcgca aatattacca
     5521 cga
//
LOCUS       DTVD01000215           19342 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_486_length_19342_cov_375.847514, whole genome
            shotgun sequence.
ACCESSION   DTVD01000215 DTVD01000000
VERSION     DTVD01000215.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19342)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_19342_cov_375.847514"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="EYO68_07410"
     CDS             complement(<1..1690)
                     /locus_tag="EYO68_07410"
                     /inference="COORDINATES: protein motif:HMM:PF12349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patched family protein"
                     /protein_id="HIC07669.1"
                     /translation="MKNIVSKIENQFEQLGYFVFAQRWKVILMALVLFGVLASQVPHI
                     VIDTSNEAFLEPDDPILTQYDAFRDQFGRDEVVVVAIQPKDVFERQFLERLKAFHDDL
                     ESEVPYLDEVISLVNVTSIKGEEEELLVEELLEDWPEDEEGIAALKKDVLSNKFYKNL
                     LISEDGTFTTVIIRSNAFTGEDTLTEDSEFGEDMFEDEGGSDSAENSEVTTLDETPEG
                     RITEQQNTEFTAAIESIVRRYTTPDFPTNVAGSPVMTGVLKDEMMSNIPRFTLMALGL
                     IGILLLLLFRTPVGVFLPLLTVIMSLISAIGLMAVSGTPFTVVTQILPSLLLAVGVGY
                     SVHLLTIYYHHLQRNGDKEKAIVFALGHSGLAILMTSLTTAGGLISFLPAPLAPVSAL
                     GLFGAIGVLLAVFYTLLFVPAVLAVLPVSKKRVSPEDDGSSLADRILGGFGMFAVNRP
                     WTVVFGSILLGLVAVGGTTQLRFSHDVISWFPEDNSIRQATEVIDKNLKGATSIEIII
                     DTGKINGVKDPDFMQRLDDFNRFAEQWKSEDPSTDVNLLVGKSTSIADTLKQIHQ"
     gene            complement(1712..2272)
                     /locus_tag="EYO68_07415"
     CDS             complement(1712..2272)
                     /locus_tag="EYO68_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIC07670.1"
                     /translation="MSKDTRKKLLEGARECLVQEGFRKTTVKSIAQYAGVNHGLVHHY
                     FGSKEELLAALVENVAKGEGTPMSAISTLVEHPAGDVSAMSSAEMQSFVLKKIVPHMT
                     SDYNKFVMEFLVMSEEMPSVAKQTQKLLNRRREWFGRLLGIQDPGLLLLVSGALMGLM
                     LQYKLDPTVDLERAVIKLFRMIYKNN"
     gene            complement(2608..3021)
                     /locus_tag="EYO68_07420"
     CDS             complement(2608..3021)
                     /locus_tag="EYO68_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07671.1"
                     /translation="MNTQKNLMMLTIVISAIYGVWAIFAPGSIMSTYGTPEEFVNPVT
                     LNIVMLFGVAAWVVAILGWHIRSTVTEENVEKAMSYFALAWLLYGLHGVISERILTWP
                     EGLEPRAFSEQTIGGIVFLVLSVVYYILRKPKGGE"
     gene            complement(3150..3476)
                     /locus_tag="EYO68_07425"
     CDS             complement(3150..3476)
                     /locus_tag="EYO68_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIC07672.1"
                     /translation="MAHYLVQASYSSGSWAAQIKNPQNRIEQVGKMFASKGIKFLSGY
                     YSFGEFDLCLILQGPDNVSAASALIAAASGGAISKIQTTVLMTAEEGLEAIKGAGSVD
                     YSPPGS"
     gene            complement(3848..5758)
                     /locus_tag="EYO68_07430"
     CDS             complement(3848..5758)
                     /locus_tag="EYO68_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HIC07673.1"
                     /translation="MKEVSLPSTWGSVVLLLICLVAVNLMAHFFPLRYDLTEERLYTI
                     SEGSRKILAGLTDPVRINFYYSRNNSELPPNFKIYAQRVQELLEEYVALSKGMVVLKI
                     LDPKPDTDEEEWAQKYGIKPITLPSGNTVYFGAVISMLDQEMLLPYFDQRRENFLEYD
                     ISQAIQKVGSTSTSKVGLLSVLNLQGGRSMIPGQPPAQKWVFLNELEKSVTVENLALT
                     IEEIPDDINLLIVLHPRSFNPRLRYAVDQYVLRGGRLVVLLDPNARIDMTSPANQFGQ
                     QPQLSSDLPELLKQWGVDYDVAKVVGDRLHATQVNTGQGVMSFPMWMTFRSDSLDQKH
                     PITAQLENLLFVEAGSLKKAADSKTEFTPLLSLSAKSGLIDTFQLRFAAPDQLSRDMK
                     VDGEAKAVMAITTGIFSSAFPNGQPVKEKKNPEASADGEESEEETPLKHPHLLEATEQ
                     NSILLFSDVDFLSDQFSVQKLNFLGQTIIQPRNDNLNLMLNAAEHLSGNEALMSIRSR
                     GRFSRPFTRLLAMQRQAQLRHQTEEKLLLSQLEEVQQRLNSLLESAGEQGQSEVILPP
                     EVQVEIEKFREEERQARRKLREVRKILRQDIERLGQGLLLLNMLLVPLIVGIIGLIVY
                     RLRTRLRLKTIR"
     gene            complement(5827..6561)
                     /locus_tag="EYO68_07435"
     CDS             complement(5827..6561)
                     /locus_tag="EYO68_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07674.1"
                     /translation="MNGFYPIFKRELLAYFRSPVAYVFIIIFLAANAGCTFFLGNFYD
                     SQQASLEIFFLFHPWLYLFLIPAVGMRLWSEERRSGTAELLLTFPVRLTTTVFAKFFA
                     AWFFIGIALLLTFPMVWTVYFLGSPDKGPIITGYLGSFLMAGSYLAIASCTSALTKNQ
                     VISFILSVNICLVLVLLGWGVLGDTLNSVFPVWISDFVSQFSFTTHYDAIRRGVIDLR
                     DIIYFVSIIVIMLMLNVVVLESHKAS"
     gene            complement(6625..7563)
                     /locus_tag="EYO68_07440"
     CDS             complement(6625..7563)
                     /locus_tag="EYO68_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC07675.1"
                     /translation="MIQVHSLSKNFNNFLAVDQVSFEVQKGEVLGFLGPNGAGKSTTM
                     KMLTCFIPPSSGTAEICGFSILENPLEVRNQIGYLPESAPSYDEMLVGEFLRFVAEIR
                     GFSGKELKRRVSIVIDMTSLREVENQMIETLSKGFRQRTSLAQALIHDPPVLILDEPT
                     DGLDPNQKHEVRTLIKNMSEDRTILISTHILEEVDAVCTRAMIISEGRVVGYGTPDEL
                     MSQSLYRNSVTVSVNAEDHIPLMNDFNKLDFVYSVEHLPEKRQGLVTMRLFPQNKESI
                     TRQLEHYLFSKKIPIEQFVVDRGRMEEVFRELTTNG"
     gene            7703..8512
                     /locus_tag="EYO68_07445"
     CDS             7703..8512
                     /locus_tag="EYO68_07445"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIC07676.1"
                     /translation="MRNKKKNPQFLKSPDTGEHHLLNPELEGDSFFWDGGEIGVLLLH
                     GFTATTAEVRPLAKRFHDAGFTVSGVLLPGHGTTPENLNETSRTEWLEASEKAYLELK
                     EKCSQIFVGGESAGAVLALHLASEHPEIKGLLLYAPAMRLASSFLRKLIMVIASPFVF
                     AIPKKIEKNSDQMPWQGYKVNPLKAGVQLLKLQSETKLRLFRIYQPILIIQANLDQTV
                     DLNSGDIILQGVQSAVRESHWMEQSRHVVILDREFKDVADISLEFLVKLIQ"
     gene            8641..10668
                     /locus_tag="EYO68_07450"
     CDS             8641..10668
                     /locus_tag="EYO68_07450"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00501.26,HMM:PF00550.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07677.1"
                     /translation="MPADKYKNIVEVARQRAMEHPDRNCVIFLEDGEKPVVGSGTSGS
                     MTYQENDHAARQVAAALQKRGIAKGERVLVILPNSLEFVKVFYGCLYAGILAVPLSEP
                     AGPQNMAAYLETFLPTLKVSKPSLLVATTLMVDFLRNQLPPELQKVFAGLAIASAEEI
                     LAEKDGEYSQPEINSTDTAYLQFTSGSTGTPKGIMIGHSNLMSNLEEARKFMQLEEEN
                     GTVVWLPLFHDFGLAAGMMGALYSGGFVVLMTPAHFIRKPLRWLTAMSKYKCAHSYVP
                     PFALDLCMKKISNDELAQLDLSCMVSVTDGSEPVHYLPTKKFNERFAECGLKPDVIRP
                     GFGMAEIVIMFSGSKTGLHGLCVDRHVLETEGRVKLQDEDAAETDKKMLVNLGSQMDG
                     HEIVIKGAENQVLPEGEVGELMVSGPSVAQGYYENKQATEEIFLQKIKGKEQPFLATG
                     DTALLWEGNLYFAGRIKDIIIIRGRNYYPHDIELVLSRVEELRPGCLMAYASGLENES
                     EHLAVAVEVRADLIKDMDVFRKYVLNSIDQQIIEIVGKHFQITPSERLYLEPGTIAKT
                     SSGKIKHLRNRQTFAEEHFDGLLERLVSSQDEEDSETDETKPSLKNEIVVLFKKVVSL
                     EPDLDQPILDCGADSVVIVEFIDQIETKYSQSFEVEDNTSLHDIIGQIKSS"
     gene            complement(10710..11540)
                     /locus_tag="EYO68_07455"
     CDS             complement(10710..11540)
                     /locus_tag="EYO68_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIC07678.1"
                     /translation="MISSKRQINIACLQTRPRPDFKSALDEAIALAEEAIEAGAEILT
                     LPEYCGGLKTEGSAFAPPFATEENHPVLKGLRDFAKNRKKYLLIGSIAIKGPAEKILN
                     RSFIIDECGNIISRYDKIHLFDITLSEKESYRESATVHGGQTAVICQTPLGCFGQTIC
                     YDLRFPHLYRDLSQSGAEILFVPAAFTKKTGEAHWHVLNRARAIENGAFVVAPCAIGR
                     VEGGGESYGHSLIINPWGEILADGGADSGFINVNINLEEVNSARNRIPSLSHDRAFEF
                     "
     gene            complement(11533..12519)
                     /locus_tag="EYO68_07460"
     CDS             complement(11533..12519)
                     /locus_tag="EYO68_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIC07679.1"
                     /translation="MTASITCSVQESEAGKRVDQLLTALDEIVSRAQAQRLIKSGNVL
                     LNGKPECAPSRKVRAGQEISVTIPPPESTEVLPEKGVLDILYEDEHLIVINKAAGVVV
                     HPSAGHASGTLVNFLIHHCQDLSGIGGIMRPGIVHRIDKDTSGVLVIAKTDDAHLHLS
                     NQFKQHSVKRQYQCLVWGVPEKEHGVVNAALGRHPVRRKDMSIVENDNPEESGREKGK
                     SAVTHWRLLQSFEFAALLACRLETGRTHQIRVHLTSIGHPVIGDPQYGKSPLKQLSGI
                     STELSQTLTNFKRQALHAEILGFEHPVSGEWLEFKAPFPDDYKILLKAIQHD"
     gene            complement(12516..13664)
                     /locus_tag="EYO68_07465"
     CDS             complement(12516..13664)
                     /locus_tag="EYO68_07465"
                     /inference="COORDINATES: protein motif:HMM:PF00155.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIC07680.1"
                     /translation="MISTSQTSHLDSIDSFLAMEVFAKAQALEAQGRDIIHLEFGEPD
                     FTAPPAAAESVKKSMEINDAGYTFSQGLEELRIEIAKKYANDYGVKILPEQVLVSNGS
                     SLLLYLAIRILAPPGSQVILTDPCYACYENVVRLAGAEPVMIKLRHEDGFQLNVDDLK
                     KRVTPKTRAMLINSPMNPTGVVFSSEVMTALAELDIPIISDEIYADLSFEDPPCSFLS
                     YSPQTVAIHGFSKSYAMTGWRLGYMITPLEWMSAADRLHQNLMISATEFVQEAGISVL
                     RKSAAYCESMKTEFNRRRLFVLERMRELGMDLGYTPTGAFYVLYKYRDSGKSSLDLCH
                     EVLEKTGVAIAPGRDFGLGAEGFVRISYAQSMENLDRALNLLAESMLL"
     gene            complement(13847..14962)
                     /locus_tag="EYO68_07470"
     CDS             complement(13847..14962)
                     /locus_tag="EYO68_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07681.1"
                     /translation="MSDIPVGAQVPSSRVELTEVPNKPLVTSKVTDSAFEKVLPFSTD
                     LELRDRYINFFGGLRLGKLLEDLDLIAGEVAYKHTEGWERGMTIVTAACDRIDLLGEL
                     RSDRDLQLLSSINWVGRSSLEVGVRISSKEGKSWVRVARAYFIMVARREGKAEPVNSL
                     EPVSKNEQRRFKQGEQRQQERRAIVQTSYLHNTPTAEESHVLHDLFLRIKNSEIEGVP
                     MQESIRQSTLLMHPQSRNVHNNIFGGYLMREAFELAWNITYLFCRKRPQFVSMDHMYF
                     YKPVEIGSIISFTGTVVYTVDKSLMVEVVTEVIRPKSGETQLTNVCYFTFNALDDSGK
                     LQQIPVILPDTYEEGLKYLDGAKRFKLGEKKRTLIKN"
     gene            complement(15017..16609)
                     /locus_tag="EYO68_07475"
     CDS             complement(15017..16609)
                     /locus_tag="EYO68_07475"
                     /inference="COORDINATES: protein motif:HMM:PF03781.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07682.1"
                     /translation="MFAPGTACSGIMTHNKRQIKIVILQIIQNINEKCKKELWLVKIM
                     SQSTFSVVFHSIIFIFIAFPEVVNAVQTQDQLVANFDSKLEITTDNIAARVLGSKKRV
                     VITVIDLFNHETQERDTQTDLLEEKFTDSLYKKLPDQVVPYFEIVYLRLEWRSTFPEI
                     KHDPLTEDIAKLTNADWLLTGTHQTNDGLLSVSLELYDLKSGDLLWQTVVGMDSTIEN
                     DLEKNAQLENGLEEEVAQKFEQNSSLFPTQNDTQLPSPLFPQITQDPALEQKKTGEYE
                     GNIVSALMIETVKERQEYSDGMVFINEGEFLMGSDLGDEDELPDHLVFVKSFYLDQHE
                     VTNADYSKCVECTRGHGGFDTIEPQQPVVYVDWKNADAFCQFQNKRLPTESEWEYAAR
                     AGSEEKYSFGDNISLLETYAWLKTNTVDKGLWGAKTVSSKKANNWGLHDLYGNVMEWV
                     QNYYKPNYFAYVRQSDNSNDLNVPENEEYPLRVVRGGAWGGLHGAGTPEGLRSAKRYA
                     FVEWTRSFQIGFRCAMDIPEKN"
     gene            complement(16573..18009)
                     /locus_tag="EYO68_07480"
     CDS             complement(16573..18009)
                     /locus_tag="EYO68_07480"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIC07683.1"
                     /translation="MVRLRFAPSPTGYLHVGGLRTALFCWFYVRQQGGKFIFRLEDTD
                     QKRLVEGAENDLIRMLEWAGIDLDEGPGIGGESGPYRQSERIEIYSRHVRKLLDDENA
                     YHCFCSAERLEKLKAGQRAKGDTPRYDGFCRRLSKAEVSRRLGEGDQHVVRMKVPEIP
                     ETLVLDDLIRGSVSIETSQTEDQVLVKSDGFPTYHLAVVVDDHLMNITHVVRGEEWLS
                     SFPKHLLLYRFLGWDPPRFAHLPLILNPDKSKLSKRQGDVAVEDFRSQGYLPEALVNF
                     ISLLGWSPAQDQELFSLSELLEEFSFERVNKSGAVFDREKLNWMNQQYIQNLNLEDFH
                     QRLLPFLAETTYAGEDEILLRKAIKILQPRLVTLAEIGDRLSLFFDQNPVATDPEVKN
                     LLRKDTSKDVLSVFIKQLESIEKLTEENFASVVGNIQKETMIKGKDLWLPLRYAITLE
                     AQGPDLKLMVDFFGKEKCLHLARHALEL"
     gene            complement(18058..19161)
                     /gene="rodA"
                     /locus_tag="EYO68_07485"
     CDS             complement(18058..19161)
                     /gene="rodA"
                     /locus_tag="EYO68_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIC07684.1"
                     /translation="MLDQRFFANIDWVLCFLVLLICGVGLIALSSATVGTPGQEDYLI
                     QQVFRILAGIGVIILVQLVHYRNWARLGFVMHLVVIGLLVLVLFYGTGGPGAPVQRWL
                     KVGPFFFQPSEFSKFTLVLALSHHFRDFEQPKGNWLWMIWPLALMAVPVVLIVKQPDL
                     GTAMLLMIVFVPIIFLMGIRLKTIFILIILALASLPIVWIYVLKSYQKGRILTFLDPE
                     RDPLGSGYHIIQSKIAIGSGGVFGKGFGEGTQGQLNFLPAHHTDFIFSVFSEEWGFFG
                     AMLVLILFLTLALWSLAEVLKSKNLVSAIQITGIVAIFISHVLINIGMTTGLMPVVGV
                     PLPFFSYGGSSMLSMMFGIGLLLNIRMRRFEKQ"
BASE COUNT         5700 a         4374 c         3841 g         5427 t
ORIGIN      
        1 cttgatggat ttgcttaaga gtatcagcga ttgaagtaga tttaccaacc agcaaattta
       61 catccgtcga cgggtcttcc gatttccact gttctgcaaa ccgattgaaa tcatcaagcc
      121 gctgcatgaa atccggatct ttcacgccgt tgatttttcc cgtatcgata atgatttcaa
      181 tcgaagtagc tcctttcaaa tttttgtcaa tgacttctgt tgcctgccga attgaattat
      241 cttccggaaa ccacgaaatc acatcatgcg aaaaacggag ttgagtggtg cctccgactg
      301 ccaccagtcc cagcaatata ctcccaaaaa ccaccgtcca gggtctgttg accgcaaaca
      361 tgccaaagcc ccccagaatc ctgtccgcca ggctggaacc gtcgtcctca ggactgacac
      421 gttttttgct gacaggcagc acagccaata ctgcgggaac aaataaaagg gtataaaata
      481 ctgccagcaa aaccccgatt gcaccaaata aacccaaagc tgaaaccgga gcaagtggag
      541 ccggtaaaaa ggaaatcaat cctccggctg tggtcaaact ggtcatcaga atagccaatc
      601 cggaatgtcc cagcgcaaag acgatggcct tttctttgtc accgttgcgc tgaaggtgat
      661 gataataaat ggtcagtaaa tgcactgaat atccaactcc tactgccaat aataatgaag
      721 gcagtatctg cgtaacaaca gtgaatgggg ttcctgaaac agccatcaaa cctatagcag
      781 aaataagaga catgataacc gtcaacaacg gcagaaaaac gccaaccgga gttcggaaca
      841 aaagcaacag caaaatcccg atcaaaccca gtgccatcaa agtaaagcgc ggaatattgc
      901 tcatcatttc gtctttcaga acacctgtca ttaccggaga accggcgacg tttgtgggga
      961 aatccggagt agtgtaacgt cggacaatac tttctattgc ggcggtaaat tctgtatttt
     1021 gctgctccgt gatccgcccc tcaggggttt catccaacgt ggtaacttca gaattttctg
     1081 cggaatcgga accgccttca tcctcaaaca tatcctctcc aaattccgaa tcttctgtca
     1141 aagtatcttc tcccgtaaat gcattggaac gaatgatcac tgtagtaaaa gttccatctt
     1201 cagaaatcag cagattcttg taaaatttgt tggagagcac atccttttta agcgctgcaa
     1261 ttccctcttc atcttccggc cagtcttcca gcagttcctc caccagcagt tcctcctctt
     1321 cgcctttgat ggaagtcaca ttgaccaaac tgattacttc gtcaagataa ggcacctcgc
     1381 tttccaaatc atcgtgaaag gctttgagtc gttccaaaaa ctgacgttca aaaacatctt
     1441 taggttgtat agctacgacg acgacttcat cacgaccaaa ctgatcacgg aatgcgtcat
     1501 actgagtcag tatcggatca tccggttcca gaaatgcttc gtttgatgta tcgataacta
     1561 tgtgtggaac ctgtgaagca agaactccaa ataaaaccag agccatcaag attactttcc
     1621 agcgctgggc aaagacaaaa tagccaagtt gttcaaattg attctcaatt tttgagacta
     1681 tatttttcat aaactattgt ttttcaatta attaattatt tttatatatc atccggaaca
     1741 actttatcac tgctctttcc aagtcaactg tagggtccaa cttatattgg agcatcaatc
     1801 ccatcaatgc tccactgacg agcagtaaca atccaggatc ctgaatgcct aaaagacgcc
     1861 cgaaccactc acgacgacga ttcaacaatt tttgagtttg tttagcaaca ctcggcatct
     1921 cctcactcat caccagaaat tccatcacaa acttgttata atccgaagtc atgtgtggaa
     1981 caatcttctt taatacaaaa gattgcattt ctgctgaact catggcagaa acatctccgg
     2041 caggatgctc aacaagagtt gaaattgcag acattggtgt accttcgcct tttgccacat
     2101 tttcaaccag tgcggccagt aattcttcct tggatccaaa ataatgatgc accagtccgt
     2161 ggttgactcc cgcatactgt gcaatagatt tcactgtagt ttttcggaag ccttcctgaa
     2221 ccaggcactc tctggcaccc tccaaaagtt tttttcttgt atcttttgac ataaataaaa
     2281 aagtgctgaa agattagctt ttgatatagt ttagtcgtac gactaatatt tgtcaaacta
     2341 aaaatgaata atctgcattc gcaaatcttt tagattcttc aagtggacaa tagtactcgt
     2401 gatctaatgt tttataccaa gtgagaaaag atgcagaatc gcatcaactt gtttgatagc
     2461 ctcaaggcct tcttagacta ttccgaggcc ctatacactt taggatggtg gagatccgta
     2521 tgccagttta ataatatctt aatgcttgaa tagatacttg gaaggtgggg ggacacaagt
     2581 tcccccctaa gataaggtta ggtttgttta ctcaccacct ttcggtttac gcaagatata
     2641 atatacaaca ctcaaaacga ggaaaactat acctccaata gtctgctctg aaaaggcacg
     2701 tggttccagt ccttccggcc aggttaatat acgttctgaa atgacgccgt gtagtccata
     2761 aagcagccag gccagagcaa aatagctcat ggctttctca acgttttctt cagttacagt
     2821 tgacctgatg tgccagccaa gaatagcgac aacccaggca gcaacaccga acaacataac
     2881 aatgttcagg gtaacaggat taacaaattc ttctggagtt ccataggtac tcataatgct
     2941 tccgggagca aaaatggccc aaactccata aattgctgaa atgacgatgg ttaacatcat
     3001 caggttcttt tgagtattca taagcctcct ttattttgga aaaaagtatt aatgactagt
     3061 gaaaaaaacc gtcaacttta ttgtcggtat tttagtatct gaaagaggcc gatctactag
     3121 accgaccgct tacagtaaat tcacagaatt caggaaccag gtgggctgta atccactgaa
     3181 ccagctcctt ttatggcctc aaggccttct tcggccgtca tcaggacggt ggtctggatt
     3241 tttgaaattg ctcctcccga agcagcagca attagtgcag acgctgcact tacgttatcc
     3301 ggaccctgca gaatcaggca aaggtcaaac tcgccaaacg agtagtatcc cgaaaggaat
     3361 ttaattcctt ttgaggcaaa catctttcct acctgctcaa tcctgttctg aggattcttg
     3421 atctgggctg cccatgaacc ggacgagtat gatgcctgaa ctaaataatg cgccatatta
     3481 cctcctttat tagttgagag agaaataaac gaaagccact gagttcatac ctcactgaca
     3541 agtatcagct taaggtatcg ctgacggctt tcatgggaga tctcccacag catttcagct
     3601 atgaaagata gttagcggaa tattgtaaac cagaaattta actagtcaag gaaaaaattg
     3661 aaattcactt ataaccggta attcaaccac aatattttcg aattctaggg cttgctgaaa
     3721 aagtatttct tttgtcatcc cggacttgat ccgggatcta taataatact gctggattcc
     3781 gggcaaagct atgctttgtc cggaatgaca attgtgcttt ttctatgaaa tagctagaag
     3841 atacaaacta gcgtatggtt ttaagccgca gtcttgtgcg gagtcggtaa acaatcagac
     3901 ctatgattcc gacgattagt ggtacgagca gcatgttcag caggagcaat ccctgcccca
     3961 accgctcgat atcctgtcgc aaaattttgc ggacttcccg caactttcgg cgtgcctgcc
     4021 tttcctcttc cctgaacttc tcaatttcaa cctgaacttc cggaggcaaa atcacttctg
     4081 actgtccttg ttcaccagca gattcgagca ggctgttcaa acgctgctgt acttcctcca
     4141 actgagaaag aagcagtttt tcttcagttt gatgacgaag ttgagcctgc cgctgcatgg
     4201 ccagtaaacg agtgaaaggt cttgaaaaac gtccccgtga acgaatgctc attagagctt
     4261 cattgccgga aagatgttcg gcggcattga gcattagatt cagattgtca tttctcggct
     4321 ggatgattgt ttgaccgagg aaattaagtt tttgtacaga aaactgatcg gagagaaaat
     4381 caacatcact gaaaagcaat attgaatttt gctctgtggc ttccagcaaa tgtggatgct
     4441 tcagaggtgt ttcttcttca ctttcttcac catctgcaga tgcttccgga ttcttttttt
     4501 ctttgaccgg ttggccattc ggaaaagcac ttgaaaatat gccggttgtt attgccatta
     4561 ctgctttcgc ctcaccatcc actttcatat ctctagacaa ttgatccgga gccgcaaacc
     4621 gtaattggaa agtatcgatc aaaccgcttt tagctgataa tgaaagcaaa ggtgtaaatt
     4681 cggttttact gtcagcagct ttttttaagg aacctgcctc aacgaaaagc agattttcca
     4741 gctgggctgt gattggatgt ttctgatcca gcgaatcgga gcggaaagtc atccacattg
     4801 gaaagctcat cacaccctgc cctgtattga cttgagtagc atgcagacgg tctccaacta
     4861 ctttggctac atcgtagtcc acaccccact gcttgagtag ttccggaaga tcgctggaaa
     4921 gttgcggctg ctgaccaaac tgatttgcag gcgatgtcat atcaattcgt gcatttgggt
     4981 ccagcagtac tacgagccgc cctccacgca acacgtactg atcgactgca tatctcaaac
     5041 gcggattgaa actacgcggg tgcaggacaa tcaacaggtt gatatcatca ggaatctctt
     5101 ctattgtcaa agccaggttt tcaactgtta ctgatttttc caactcattg agaaaaaccc
     5161 atttttgtgc tggaggctgc ccgggaatca ttgaccttcc tccctgaaga ttcaagacac
     5221 tcagcagacc aaccttggaa gttgaggtgg aaccgacttt ttgaatggcc tgggaaatat
     5281 catattctaa aaaattttcc cgacgctgat caaaatatgg cagtagcatt tcctgatcca
     5341 gcatggaaat cactgctcca aaatataccg tgtttcccga aggcagagta atgggtttga
     5401 ttccatattt ttgtgcccac tcttcttcat cagtatctgg ttttggatcc aaaattttca
     5461 gaactaccat gccctttgaa agtgcaacat attcttcaag cagttcctgt acacgttgtg
     5521 cataaatttt gaagtttgga ggcagttcag aattgtttct ggaatagtaa aaatttatcc
     5581 tgactggatc agtcaaccct gccagtatct tcctggaacc ttctgaaatt gtgtaaaggc
     5641 gctcttctgt cagatcataa cgtaacggga aaaaatgagc catcaaattt actgccacta
     5701 gacaaatcaa cagcaggaca acactgcccc aggtactggg taacgaaact tccttcatta
     5761 tgttttatag ttcaaagttc ttttaagcag attttaggtt aataataaaa tgagttctgt
     5821 gttaaatcag gatgctttgt gcgactcaag aaccacaaca ttcaacatca gcataatgac
     5881 aataatagat acaaaataaa ttatatccct gagatcaatc actcctctgc gaatcgcgtc
     5941 atagtgtgtg gtgaaactaa actggctgac gaaatcgctg atccagacag gaaaaaccga
     6001 atttaatgtg tcacctaaaa caccccatcc taacaaaacc agcaccagac aaatgttgac
     6061 gctcaggata aaactgatta cctgattttt tgtcagagca gaggtgcagc tggcaatagc
     6121 aaggtaggaa cccgccatca aaaagctgcc taaatatccg gtaatgattg gtcccttatc
     6181 cggacttccc aaaaaataaa ctgtccaaac cattggaaaa gtgagcagca aagcaattcc
     6241 aataaaaaac catgctgcaa aaaactttgc aaagacagta gttgtaagtc ttacaggaaa
     6301 agtgagcagc aactctgcag ttccagaacg acgttcctct gaccagagac gcataccgac
     6361 tgcaggaatc agaaataaat aaagccaggg atggaagagg aaaaagattt caagacttgc
     6421 ctgctgcgag tcgtaaaaat ttccaagaaa aaaagtacaa cctgcatttg ctgccaaaaa
     6481 aataatgatg aaaacatagg ccaccggcga acgaaagtat gccagcagtt cccgtttgaa
     6541 tattggataa aatccgttca tagacaatct tttagatttc agtttctgtt gtaagtaaaa
     6601 tgacaggtta tcagcttcca gtttttagcc gttggtcgta agttctctaa agacttcctc
     6661 cattcgccct cgatctacta caaactgctc aataggtatc ttttttgaga acaaataatg
     6721 ctcaagctga cgcgtaattg attctttatt ttgtgggaac aaacgcatag taaccagtcc
     6781 ctgacgtttt tccggaagat gttcaacaga ataaacaaaa tcgagtttat taaaatcatt
     6841 catcaagggg atatgatcct ccgcgttcac actaactgta acggagttac ggtaaagaga
     6901 ttgagacatt aattcatcag gtgttccata gcccacaacc cgaccttctg aaatgatcat
     6961 ggcacgtgta cagacagcat caacctcttc taaaatatgt gtagaaatca aaatagtcct
     7021 gtcttcactc atatttttga ttaaggtacg tacctcatgt ttttgatttg gatcaaggcc
     7081 atcagtcggc tcatcaagaa tcagaacagg tggatcgtga attaatgctt gtgccaaact
     7141 tgtacgctgc cgaaagccct ttgaaagagt ttctatcatt tgattttcga cttctctaag
     7201 tgaagtcata tcgatcacaa tgctaacccg cctttttaac tcttttccac tgaatccccg
     7261 aatttctgca acaaaacgga gaaactctcc aaccagcatt tcgtcataag aaggagcact
     7321 ttcaggaagg tatccgattt ggttgcgaac ctccagggga ttttccaaaa tgctgaaacc
     7381 acaaatttct gctgtacccg agcttggtgg aatgaaacag gtcagcattt tcatggttgt
     7441 cgatttacct gctccgtttg gaccaaggaa acccaggact tcacccttct gaacttcaaa
     7501 tgaaacttga tcgactgcca ggaagttgtt aaaatttttg ctcagtgagt gaacttgaat
     7561 cataaaaaac tgtacttaaa tttctagctg tttgggattt ataaataaaa catagaattc
     7621 tatatctttc ttaaaattgt atcaagacaa aacacataac tcagctctgt gtattgcgta
     7681 acaatcgaag tcaggaaaga aaatgagaaa taagaagaag aatcctcaat tcttgaaatc
     7741 accagatact ggtgagcatc acctgctcaa tccagagttg gaaggagatt catttttttg
     7801 ggatggtgga gagatcggag tacttttgct gcacggcttc actgcaacca cggcagaggt
     7861 acgaccactg gcaaaacggt tccatgatgc tggatttact gtttcaggag tcctgcttcc
     7921 aggtcatgga acaacaccgg aaaatctgaa tgagacttca cgcacagaat ggcttgaagc
     7981 cagtgaaaaa gcatatttag aattaaagga aaaatgttct caaatatttg tgggaggaga
     8041 atcagctgga gctgtacttg ctcttcactt agccagtgaa catcctgaaa taaaaggtct
     8101 tttactttat gcgccggcca tgcgattggc ttcctctttc ctccgaaaac ttataatggt
     8161 catagcctca ccttttgttt ttgccatccc aaagaaaata gaaaagaaca gcgatcaaat
     8221 gccatggcag ggttacaaag tcaatccctt aaaagcaggg gttcaacttt tgaaattaca
     8281 gtcggaaact aagctccgtc tgtttcgaat atatcaacct attttgatta ttcaggcaaa
     8341 tttggatcaa actgtagatt taaatagcgg tgacattatt ttacagggag ttcaatctgc
     8401 agtccgtgaa tctcactgga tggaacaatc aagacatgta gtcattctcg accgggagtt
     8461 taaagatgta gcggatattt cattggagtt tttagttaaa ttaatccaat aagcgaaaac
     8521 cgttttctat atagctctca gtctttgaac attttttctt gaggccactc tagaatgtgt
     8581 taaactgtgc agcaaagaaa aataaattca atttgcagat caatcagccc atagataatt
     8641 atgccagcag acaaatataa aaacatagta gaagtggccc gacaaagggc aatggagcat
     8701 ccggacagaa actgtgttat tttccttgaa gatggagaga aaccggttgt cggcagcgga
     8761 acttccggtt ccatgactta ccaggaaaat gaccacgctg ctcgtcaggt tgccgcagcc
     8821 ctgcaaaaac gcggaattgc aaaaggtgag cgcgtgctgg ttattcttcc caacagtctg
     8881 gagttcgtca aagtttttta cggctgtttg tatgcaggaa ttcttgcagt tcccttgtca
     8941 gaaccagcag gaccgcaaaa catggcggcc tatctggaaa cgtttttgcc gactttaaaa
     9001 gtcagcaaac caagtttgct ggttgcaaca accttgatgg tggatttttt acgtaaccag
     9061 ttgcctccgg aactgcaaaa agtttttgcc ggacttgcaa ttgcgtctgc agaagaaatt
     9121 cttgcagaaa aggatggcga atattctcaa cccgaaatta attctaccga cactgcgtat
     9181 ttacagttca cttccggaag tacaggaact cctaaaggca tcatgattgg gcacagtaat
     9241 ttgatgtcaa atttggaaga agcccgcaaa tttatgcagc tggaagagga aaacggaacc
     9301 gtggtctggc tgccgctctt tcacgatttt ggtttagccg cgggaatgat gggggcactt
     9361 tattccggag ggttcgtggt tttgatgaca cctgcacatt tcatccggaa gcccctgcgc
     9421 tggctcactg cgatgtcaaa gtacaagtgc gcgcacagct acgttccacc atttgccctc
     9481 gatttatgta tgaaaaaaat atcgaatgac gaacttgctc aactcgattt atcctgcatg
     9541 gtttctgtaa cagacggctc tgagccggtt cattatttac caaccaaaaa atttaatgag
     9601 cgttttgcag aatgtggctt aaagccggat gtaatccgtc ccggatttgg tatggcggaa
     9661 atagtgatca tgttttcggg cagtaaaacg ggtctgcatg gactttgcgt tgatcggcat
     9721 gttttggaaa cagaaggacg ggttaaatta caggacgagg atgctgcgga aacagacaaa
     9781 aagatgcttg tcaatttggg ttcgcaaatg gacggtcacg aaattgttat caaaggtgca
     9841 gaaaaccagg tacttccgga aggagaagta ggagagctga tggtaagcgg cccctctgtt
     9901 gcccagggat attatgaaaa caaacaggct actgaagaaa tttttctgca aaaaattaaa
     9961 ggcaaagaac aaccatttct ggcaaccggc gacacagcac tgctctggga ggggaacctt
    10021 tattttgcgg gcagaatcaa agacatcatc atcattcgcg gacgaaatta ttatccgcat
    10081 gacatcgaac tagtgctttc aagagtagaa gaattacggc cgggatgctt gatggcttat
    10141 gcttcaggat tagaaaatga gtcggagcat ttagcggttg ctgtggaagt tcgtgctgat
    10201 ttaattaaag acatggatgt tttccgtaaa tatgttttga attccattga tcagcaaatc
    10261 attgaaatcg tcggtaaaca ttttcagatc acaccaagtg aacgcctgta tttggaacct
    10321 ggtactattg ccaaaacttc cagtggaaaa ataaaacacc tgcgaaaccg tcaaactttt
    10381 gcagaagagc attttgatgg gcttttagag cggctagtaa gttcacagga cgaggaagat
    10441 tcagaaactg acgaaacaaa accttcactg aaaaatgaga ttgttgtact ctttaaaaaa
    10501 gttgtttctc tggaaccgga tttggatcag ccgattttgg attgtggtgc agactcggtg
    10561 gtgattgttg agtttattga ccagattgag acaaaatact cacagagttt tgaggttgaa
    10621 gataatacca gcctgcatga tattatcgga cagattaaat ctagttagga tatatatttt
    10681 tgttaagtat tttgtaacct ttccccaatc taaaactcaa atgctctgtc atgactcagg
    10741 cttggaattc gatttcgtgc agagttaacc tcttccagat taatatttac attgataaat
    10801 cctgaatctg cacctccatc agcaagtatt tcaccccagg gattgatgat caatgaatga
    10861 ccatagcttt caccgccgcc ctcaacccta ccaatagcac atggtgcaac cacaaaagca
    10921 ccgttttcaa ttgcacgcgc cctgtttagc acatgccagt gagcttcacc tgtttttttt
    10981 gtaaatgctg caggaacaaa caatatctct gcgcctgatt gtgataaatc acgataaaga
    11041 tgtgggaaac gtaaatcgta acagattgtc tgtccgaagc atcctaaagg cgtttggcaa
    11101 attacggctg tctgaccgcc atgaacagtg gcactttcac gatatgattc cttttccgac
    11161 agtgtgatgt caaacaaatg aattttgtcg tagcggctga tgatgttccc acattcatca
    11221 ataataaaac tgcgattcaa aatcttttcc gcgggacctt ttatagcaat tgaaccaata
    11281 agcagatatt ttttccggtt ttttgcaaag tccctgagac cttttaaaac tggatgattc
    11341 tcctcagttg caaatggtgg agcaaatgca gaaccttctg ttttcaaccc gccgcaatat
    11401 tccggtagcg taagaatttc cgctccggct tctatcgcct cttctgcaag tgcaattgct
    11461 tcatcaagag ctgatttaaa atccggtctt ggacgggttt gaagacaggc gatattgatc
    11521 tgtcttttcg aactaatcat gctgaattgc tttcagtaaa atcttgtaat catccggaaa
    11581 gggtgccttg aattccagcc attctccgga aacaggatgt tcaaatccca aaatttctgc
    11641 gtgcaaagcc tgacgcttaa aatttgtaag cgtctgactt aattctgtgg aaatccctga
    11701 aagctgctta agaggcgatt tcccatattg cggatcacca attaccggat gaccaatcga
    11761 agtcaggtgc acccgaattt ggtgggtacg ccctgtttcc aaccgacagg ccagcaaagc
    11821 tgcaaattcg aaactctgca aaagacgcca gtgagttact gcagatttcc ctttctccct
    11881 gccgctttcc tccggattat cgttttcaac aattgacata tccttgcgcc gcaccggatg
    11941 acgccccaaa gccgcgttga ccactccatg ttctttttcc ggaacacccc aaaccaggca
    12001 ttgatactgc cgtttgactg aatgctgctt aaactgattt gacaaatgca gatgagcatc
    12061 atccgttttt gcaatcacca aaactccgga ggtgtctttg tcgatccggt gtacaattcc
    12121 gggacgcata attcctccga ttccggaaag atcctgacaa tgatgaatta agaaattaac
    12181 cagagttccg gaagcatgtc cggcagaagg gtgaaccaca actcctgcgg ctttgttgat
    12241 aacgattaaa tgttcgtctt catataaaat atcaagcact cccttttccg gaagaacttc
    12301 cgttgattcc ggcggaggaa ttgtaactga aatttcctgt cctgcacgta ctttccgcga
    12361 aggcgcacat tccggttttc cgtttagtaa aacattaccg gattttataa gccgctgtgc
    12421 ctgtgcccgt gaaactattt catccaacgc tgtcaacagc tgatctaccc tctttcctgc
    12481 ttcactttct tggacagagc atgtgatgga agcagtcata acaacatact ctctgctaag
    12541 agattcaagg cacggtctaa gttttccatg gattgcgcgt agctgatccg gacaaaacct
    12601 tccgctccaa ggccaaaatc ccgtccgggt gcaatcgcaa cacccgtttt ttccagcact
    12661 tcatgacaaa gatccagaga agattttccg gaatcgcgat acttgtaaag cacataaaac
    12721 gcacctgttg gagtatatcc cagatccatt ccaagttcgc gcatgcgttc caaaacaaac
    12781 agtcgtctgc gattaaattc cgttttcata ctttcacagt aagctgcact tttacgcaaa
    12841 acggatattc ctgcttcctg tacaaactct gttgcagaaa tcatcaggtt ttgatgcaga
    12901 cggtctgccg cagacatcca ttccagagga gtaatcatgt atccaagtcg ccaacctgtc
    12961 attgcatagg attttgaaaa accatgaatt gccaccgttt ggggcgaata acttaaaaag
    13021 ctgcacggcg gatcctcaaa actcaaatcc gcatatattt cgtcagaaat tatcggaata
    13081 tccaattcgg cgagggccgt catcacttcg gaagaaaaga ccacaccggt aggattcatt
    13141 ggtgaattta tgagcatggc tctggtttta ggtgtcactc tttttttaag atcatcaaca
    13201 ttcaactgaa aaccatcttc gtgcctcaac ttgatcataa caggctctgc accagccagc
    13261 cgtactacat tttcgtaaca ggcataacaa ggatcagtga gaatgacttg gcttccgggt
    13321 ggagcaagta ttcgaattgc caaatagagc aaaagcgatg acccattgga aacaagaact
    13381 tgttccggaa gaattttgac tccgtaatca tttgcgtatt tttttgcaat ttcgattcgc
    13441 aactcctcca gaccttgaga aaaggtatat ccagcatcat taatttccat actttttttg
    13501 acagattctg ctgcagccgg aggtgcagta aaatcaggtt caccaaattc aagatgtatg
    13561 atatctcgac cttgggcttc aagtgcctgt gctttggcaa aaacctccat tgccagaaat
    13621 gaatcgatgg agtcgaggtg tgatgtttga gaggtactga tcatatttta gcagttatgt
    13681 tgtgtgatcg tgattgagag ttacagtgct ttaaaaaagt ttcctttgtc atcccggact
    13741 tgatccggga tccagcagac ttgcagcggt ttccactgga ttccgggtcc ccgcttcgct
    13801 gcccggaatg acactggata agattcgatt tctgttttct tagtgcctaa tttttgatta
    13861 atgtgcgttt tttctcacca agtttaaagc gttttgctcc atcaaggtat ttcaaacctt
    13921 cttcataagt gtctggtaaa atgaccggaa tctgttgcag ttttccggaa tcatccaggg
    13981 cattaaaggt aaaatagcat acattagtga gctgtgtctc tccggatttt ggtcgaatga
    14041 cctctgtcac aacttcaacc atcaatgatt tatcaaccgt gtaaacaact gttccggtaa
    14101 acgaaataat tgaaccaatt tcaaccggct tgtagaaata catgtgatcc atgctgacaa
    14161 actgcggcct ttttctacag aaaagataag taatattcca agccagttca aaagcctccc
    14221 gcatcaggta gccgccaaaa atattgttat gtacattgcg ggactgtggg tgcatcaaca
    14281 atgtggattg ccgaattgac tcctgcatgg gtacaccttc tatttcgcta ttcttgatcc
    14341 gtaaaaacag atcatgcaga acatggcttt cctctgccgt aggtgtgtta tgaagataac
    14401 ttgtttgcac tattgcccgt cgttcctgct gacgctgctc tccctgctta aaacgccgtt
    14461 gctcattctt gctcactggt tcaagtgaat tcaccggctc agcttttcct tcacgccggg
    14521 caaccataat aaaatatgca cgggctactc ttacccatga ttttccttct ttagacgaaa
    14581 tacgaactcc aacttccagt gaactgcgtc ccacccagtt gatgctggaa agcagctgca
    14641 gatcacggtc actgcgcaat tcgcccaata aatcaatgcg atcgcatgca gctgtgacta
    14701 ttgtcatacc tcgttcccaa ccttctgtgt gtttgtaggc aacttctcct gcaatcagat
    14761 ccaaatcttc cagcaatttc cccaggcgca atcctccaaa gaaattaata taacggtcac
    14821 gcagttccaa atctgtactg aaaggcaaaa ccttttcaaa tgcagaatcc gttactttag
    14881 atgttactag aggcttattc ggtacttcag taagttctac cctggatgac ggaacctgtg
    14941 ctccaactgg aatatcagac ataataaata tcagcaatca aggaaaaagg agggttgata
    15001 attttattca aaagttttaa tttttttcag gaatatccat tgcacagcgg aaaccgattt
    15061 gaaaagaacg cgtccattct acaaaagcat aacgttttgc ggaccgtaaa ccctctggag
    15121 tgccggctcc gtgaagtcct ccccatgcac cgcctcttac tacgcggagc ggatattctt
    15181 cattttccgg aacattcaga tcattggaat tatcggattg acgaacgtag gcaaaataat
    15241 tcggtttgta ataattttgc acccattcca taacgttgcc atacaagtca tgcagtcccc
    15301 agttgtttgc ctttttgctg ctcacagttt ttgctcccca aagaccttta tctactgtgt
    15361 ttgttttcag ccaggcgtag gtttctagca atgaaatatt gtccccaaaa ctatactttt
    15421 cctcacttcc tgcacgagcc gcatattccc actctgattc tgtcggcaaa cgcttgtttt
    15481 ggaattgaca gaaagcatct gcatttttcc aatcaacata cactaccggt tgctgaggtt
    15541 caatagtgtc aaagcctcca tgtccccgtg tacattcaac acacttgctg taatctgcat
    15601 ttgtcacctc atgctggtca agataaaaac ttttcacaaa aaccaaatga tccggtagtt
    15661 catcttcgtc accaagatca ctgcccatca aaaattcgcc ttcattaata aataccatgc
    15721 catctgaata ttcctgtctt tctttaaccg tttcaatcat cagagcagac acaatatttc
    15781 cttcatactc acctgtcttt ttctgctcca aagctggatc ttgagtaatt tgaggaaata
    15841 aaggactagg aagctgagtg tcattttgag taggaaaaag cgaactgttt tgctcgaatt
    15901 tctgcgcaac ctcttcttca agtccatttt ccaactgtgc atttttttca agatcatttt
    15961 caattgtact atccattcca actactgttt gccaaagcag gtctccactc ttcaaatcat
    16021 aaagttccaa actgactgag agcaaaccgt cgtttgtctg gtgagttccc gtaagcaacc
    16081 agtcagcgtt cgtaagtttt gctatatctt ctgtaagcgg atcatgcttt atttcaggga
    16141 aagtacttct ccattctaaa cgcaggtaaa ctatttcaaa ataaggaact acttgatcag
    16201 gtagtttttt atagagagaa tctgtaaatt tttcttcaag caaatcggtc tgcgtgtccc
    16261 tctcctgagt ctcatgatta aagagatcaa tcacagtaat taccactctc tttttacttc
    16321 caagaacccg tgctgcaata ttatccgtag ttatttccag cttggaatca aaattagcaa
    16381 ctaattgatc ctgggtttga actgcattaa cgacttcagg aaaggctata aaaataaaga
    16441 taatagaatg aaaaacaaca gaaaaagtgg attgagacat tatttttacc agccacaatt
    16501 cttttttgca tttttcattt atattttgaa taatctgcaa aattactatt tttatttgcc
    16561 ttttattatg tgtcataatt ccagagcatg ccgtgccagg tgcaaacatt tttccttccc
    16621 aaaaaaatca accatgagct ttaaatccgg accttgagct tcgagagtaa ttgcgtaacg
    16681 caaaggcagc cacaggtctt tacccttgat cattgtctct ttttgaatat ttcccacaac
    16741 actagcaaag ttttcttcag tcaatttttc aattgattcc agctgcttga taaaaacaga
    16801 aagcacatct tttgaggtat ccttccgcag gaggtttttt acttccggat cagttgctac
    16861 cggattttgg tcaaaaaata atgagagacg atcaccaatt tctgccagcg tcaccaagcg
    16921 gggctgcaga atttttattg ctttcctgag caggatttca tcctcgccgg cataagtcgt
    16981 ttctgctaag aatggaagca gtcgctggtg gaaatcttcc agatttaaat tttggatata
    17041 ttgctgattc atccagttaa gtttttcccg atcaaaaaca gctccggatt tgttaacacg
    17101 ttcaaaactg aattcttcca gcagttcgga aagtgagaac aactcctgat cctgcgcagg
    17161 tgaccagccc agcaaagaaa taaaattgac cagtgcttcc ggaagatagc cttgagaacg
    17221 gaagtcttcc acggcaacat caccttgacg tttactgagt ttggatttgt caggattaag
    17281 aattaaaggc aagtgtgcaa accggggagg atcccagccc agaaagcgat acagaagcaa
    17341 atgttttgga aatgaagaaa gccattcttc acctcgtaca acatgcgtta tattcatcaa
    17401 gtggtcgtca actacgacag ccaggtgata tgttgggaag ccgtcgcttt tgactaaaac
    17461 ctggtcttca gtttgactgg tttcaattga gacactaccc ctgattaaat catcaagcac
    17521 cagtgtttcc ggaatttcag gtactttcat ccgtacaaca tgctgatcac cctcacccag
    17581 ccgtctactg acttccgcct ttgacaggcg cctgcaaaat ccatcataac gcggagtatc
    17641 tcccttcgca cgttgtcctg ctttaagttt ttccaatcgt tccgcagagc aaaaacaatg
    17701 atatgcattt tcgtcatcaa gtaatttccg gacatggcga ctgtatattt caatgcgctc
    17761 tgattgacga taaggacctg attcacctcc aattccggga ccttcgtcca gatcaattcc
    17821 tgcccattcc aacattctga tcaaatcatt ttcggcacct tcgaccagcc ttttttgatc
    17881 agtgtcttct aaacggaaaa tgaatttgcc gccttgttga cgtacataaa accagcaaaa
    17941 aagagcagta cgtaaaccac cgacatgcaa atatcctgtg ggacttggag caaaacgaag
    18001 acgaaccatc gtaacaataa gaaggaatta aggctgagaa tctggagttt aagttattta
    18061 ttgtttttca aaacggcgca ttctgatgtt cagtaaaagg ccaattccaa acatcatgga
    18121 gagcattgaa gaaccgccat aactaaaaaa tggaagtgga acacctacaa ccggcatcaa
    18181 tcctgttgtc atgccaatat tgatcaatac atgtgatatg aatattgcga caatcccggt
    18241 aatttggatg gcacttacaa gattttttga ttttagcact tctgccaatg accacaatgc
    18301 aagtgttaaa aacagaatca ataccagcat tgcaccaaaa aatccccact cttcagaaaa
    18361 tacagaaaaa ataaaatcag tgtggtgtgc aggcaaaaaa ttcagttgcc cctgtgttcc
    18421 ttcgccaaat cctttaccaa acactcctcc actgccgata gcaattttgg actgaattat
    18481 atgatagccg gaccccagag gatcacgttc cggatcaaga aaagtcaaga tacggccttt
    18541 ttgatatgat tttagaacat aaatccagac gatgggcagg gaagccaatg cgagaataat
    18601 caggataaaa atagtcttga gacgaatacc catcaaaaaa atgattggta cgaaaacgat
    18661 catgagtaac atggctgtac ccaagtcagg ctgtttgaca atcaaaacca ctggaactgc
    18721 cattaaagct agaggccaaa tcatccaaag ccagtttcct ttaggctgtt cgaagtccct
    18781 gaaatgatgt gaaagtgcca gcaccagcgt aaacttgctg aactcagaag gttggaaaaa
    18841 aaacggccca actttcagcc agcgttgtac aggtgctcca ggtccacctg ttccataaaa
    18901 cagcacgaga accagcaacc caattactac cagatgcata acaaaaccca ggcgcgccca
    18961 atttcgatag tgaaccaatt ggactaaaat aattactcca attcctgcta aaatacgaaa
    19021 aacctgctga attaaataat cctcctgccc cggcgtaccg actgttgctg aggaaagtgc
    19081 aatcaaaccc acaccgcaaa tcagcaaaac taaaaaacat agaacccaat ctatgttggc
    19141 aaaaaaacgt tgatccagca tctctaattt attttaaatt ttaattccca gttttttatt
    19201 taattcgtga gtcatgaatt ctagtaagct tcagtattcg ctttcaattt tttctatcaa
    19261 cttttaaccg gaaacgagct tttagcattc attctacttt tgttgctaat aattcccatg
    19321 aacatcaatt cattcccagc ca
//
LOCUS       DTVD01000216           19135 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_496_length_19135_cov_238.679403, whole genome
            shotgun sequence.
ACCESSION   DTVD01000216 DTVD01000000
VERSION     DTVD01000216.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 19135)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19135)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19135
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_496_length_19135_cov_238.679403"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /gene="nusA"
                     /locus_tag="EYO68_07490"
     CDS             complement(<1..1393)
                     /gene="nusA"
                     /locus_tag="EYO68_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIC07685.1"
                     /translation="MDHEVVIKLVEESLIQAAQRKLPYREIEGHIDEDTGKINLFHFK
                     EVVELVEDPHNEISVDEVFEIDPDAGLGDEVEYEISDREFQRIAHSTRPIIFGSLKEA
                     ERQMVVSIFTDKVGDVLTGTVLRVEPNGRVAFSFQNNVEAYLFRREQIYGENFSFGDH
                     VRVLLLDVNDNPHKDSQLTISRTHPGLLIKLFEMEVPEIYDGIVKIVNASREPGRRAK
                     ISVYSTDDDVDPVGSCVGMRGSRVQAIVNDLNGEKIDIVRWSEDIDQYTCNALAPAEI
                     DSLEIDEDAQQIDVVVAPSQLSLAIGHKGQNVRLASKLIGYKINIVATEEDELSIDEQ
                     LEKEFAKTKENGLKSIDKSEDLPESDTDEAKETATALENQDDNQDSSPLTAEESETTE
                     DKDKVEAEETDNTEDTKNNSKDAPAESKTEVESKAETKTKTKAETKTKTKAETKTKTK
                     AETKTKTKAETKTK"
     gene            complement(1493..1960)
                     /locus_tag="EYO68_07495"
     CDS             complement(1493..1960)
                     /locus_tag="EYO68_07495"
                     /inference="COORDINATES: protein motif:HMM:PF02576.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIC07686.1"
                     /translation="MTQIAEKLINLLTVPIKSEGYELLDVEYNKHGSNQTVQLIIDSI
                     NGIGIDDCVAVNRIAQEVLDNENPISDSYSIEVSSPGLFRKLKTAEHYKTFTGERIRV
                     RLQQKIQGVKNAVGKLEESTEQEIRLKLESDGSELVIPYTLIKKANLEPELKF"
     gene            complement(2063..3121)
                     /locus_tag="EYO68_07500"
     CDS             complement(2063..3121)
                     /locus_tag="EYO68_07500"
                     /inference="COORDINATES: protein motif:HMM:PF00348.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIC07687.1"
                     /translation="MKIFAKDHVQLKSYLKAASEWIGEETLRCIPDGEPKEYLYDLMR
                     DYPQRGGKRFRPALVLLSCELFGGDPEKALTSAVALELFHNFALVHDDIEDESLLRRG
                     EQTLHLQHGIALALNAGDALLGLVHETLLENHSKLDSNLAVEVHQHLNKVMQATFEGQ
                     ALDIGWVANQTFPDRAEYREMISRKTGWYSGRGPCICGALIAGASKTELEAAGRFGEA
                     IGIGFQIRDDLLNLTEKSETEAPSAGAGGYGKEYGGDIAEGKRTLISIELLERLSAKD
                     AERVRTILLQTREHVTHADIDWFISQAESTGALESVASFCQEQATLASAALENLPVNP
                     ASSLLGELVEYLTIERKA"
     gene            complement(3181..4779)
                     /locus_tag="EYO68_07505"
     CDS             complement(3181..4779)
                     /locus_tag="EYO68_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07688.1"
                     /translation="MKQNNFLYTLLKWPITATVFLFTSVLQADPPEIKPWFQQAVLLK
                     SYQQSFDWRTPWEKREIRTQSGMALVVRLPVSVRISVSENGNNNNLYLLTTAEIVSDA
                     TLIEVTRKDIRSPFQAKLVLMDYAANLALLQINNSKFWRNLRPVKWTPVKSSTDHEAK
                     DIYSLKIKSQDEWTMESGTIDRMAVGHREVSNAWFPLLRLNGISQSGQGYPLLQDNET
                     AGMILDAGTGEAKAMPAAMLLEFLKHAGSNPYRSLAHRGFIWSRLPQSSTYNHFQIPP
                     EKPGIWISRVLPYGTGSDVLMQGDYLTKLGKWTLSHDGKVEHPDWGISLFDLLFLAHL
                     KVGDSVKLEVIREGKPVVLQTKVSAYEENSRSVPLKIVGTAPRYVIEGGFLFQELTLN
                     YLRIWGANWQTRAPMRLRLFLEQNKAVSIYENNKTDISSTGTKISSEHPPRIVLVSQV
                     IPDPLNIGYQKLSNAVVLQVNGKQIRRLEDVSTAFLSPINNFHRIDFLPGSERLSVIL
                     PVAELADANQRIKNNFRIPKLQSL"
     gene            complement(4776..6308)
                     /locus_tag="EYO68_07510"
     CDS             complement(4776..6308)
                     /locus_tag="EYO68_07510"
                     /inference="COORDINATES: protein motif:HMM:PF00089.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIC07689.1"
                     /translation="MNGFRCEFLLKDNPQFRKYFWIILLIECVWLALVSSVPASQNSI
                     EQSVFRITNYQQSPDWKSPWKMKPTSQGQGSGFLIHDNWILTNAHVVSDSRMLLVNKI
                     SSPEPFLADVLAIAHDSDLALLKVRDPKFYQDLKPLELGGIPELRSRVRAYGYPVGGK
                     ELSRTEGVVSRIEFGTYVHPGIDSHLLIQTDSAINPGNSGGPVTQSGQAIGVAFQSNL
                     RLNDVGYFIPVPLINRFLADIKDGRYDGVPEIGIETSSLINQHYRQYLGLPEDTGGIL
                     VERVVPHSSADGVLLIGDVLTKIEDLQIDAAGMVRYSEQQVTFFIEAENRQIGDSLQL
                     QVWRKGKFINLTLTLKAAPFGSEMRNSYDELPEYVIFGGLVFIALNRNYIHSPGNITP
                     PLAYEHWYREIERPRTRHEQVVIVTRVLPSPVNSGYTNLHNFIVSSLNGKPVRSLAHL
                     EKMLKNMPPETTNVVFGSEWHKIPLVLNFKESLEQHNSVLKRYGIIDGSRIYADKNKD
                     SQ"
     gene            complement(6305..7150)
                     /locus_tag="EYO68_07515"
     CDS             complement(6305..7150)
                     /locus_tag="EYO68_07515"
                     /inference="COORDINATES: protein motif:HMM:PF12850.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIC07690.1"
                     /translation="MLIAQISDLHIPRKNTKTYGIAPMAENLVLCIDHINQHLPKPHL
                     VLVTGDITSTGQAEEFNHAANLLNRFEMPFYVIPGNHDDRDILRSTFGKQACPVESEG
                     KIDYVIEDSDLRLIALDSTLPGSPGGEITEAQASWLDARLNEKPTQPTMIFMHHPPIK
                     CDVLETDVDGFIGKELLGSVISKHHHVEKIICGHIHVPINTSWNDTVISTAPSMGMQL
                     VLDLKLKRESKFVLEAPAYHAHYWTTDKNLITHTVVVKPVDGPYLFEEQNVIRSQQPY
                     TLFYP"
     gene            complement(7161..7967)
                     /locus_tag="EYO68_07520"
     CDS             complement(7161..7967)
                     /locus_tag="EYO68_07520"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_661233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIC07691.1"
                     /translation="MDWIQAIVLGIVQGLSEFLPISSTAHLRIVPELLGWSDPGTEFS
                     AVIQLGTLVAVLLFFRKDVVQLSSAALESLWHRNLFETPESKMAWSIAAGTIPVVVLG
                     LGFKDFIKNEARELWVIGTALIVLAIFLYLAERLSSRNRDIKQLSFLQIQLIGLTQAL
                     ALIPGCSRSGSTIMGGLMVGLNREAAARFSFLLGLPAIFGSGLYELKELMEIGISSDQ
                     YINLILGILAAFVVGYLSIELLLRFLRNHGTLVFVIYRIVLGTGILIFLV"
     gene            complement(8176..9195)
                     /gene="waaF"
                     /locus_tag="EYO68_07525"
     CDS             complement(8176..9195)
                     /gene="waaF"
                     /locus_tag="EYO68_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIC07692.1"
                     /translation="MPESAPVKILIRTPNWLGDMVMSSGFVRAVLEAFPESQVDLIVK
                     SGFEKIPLPQRGQIIPFDPKKETAGNFGKTLRAENYDYFYVLPPSFSAAWMAHKSKIP
                     HRIGYSGEFRSFLLSLAKKPEATPRSQHLLKEYLNLLAPGISTEKYPPRLEITHEWVE
                     KQLGSLEISLPESFFVFTPGAIFGPAKQWPLEHFRTLATLLHNVFGDPILILGTEADV
                     KSGAEISLGLDFVQNYCGQTSLSELLAILAKAKLLVGNDSGSMHLMSALQRPQIAIFG
                     STSPDWTAPINPNATVLSRNLSCSPCFSRTCRFAHYDCLKLIEPELVLEETLKLLTEK
                     NQTEA"
     gene            complement(9634..11118)
                     /locus_tag="EYO68_07530"
     CDS             complement(9634..11118)
                     /locus_tag="EYO68_07530"
                     /inference="COORDINATES: protein motif:HMM:PF08299.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIC07693.1"
                     /translation="MELSHNQSLEFKECLRRSLPSHVSQSWLDSLQLLKITPSKVVLG
                     GIQHKIYRYEIKTNHESLLKEVLNELFPEKAPFSEKKFEYKIFSCTGRKKSVQTEFDL
                     NQDQIANKNNDLEIKNSKSDFATEHAAGSTSSYSLDSFIPGKRNLLASRACKAVVEMP
                     GIAFNPFVIYGESGSGKTHLLEGISNELQQSNPKKQRVQVSAEDFLNDFVNHLRLSKM
                     KEFRDRYRKADAFLLDDLQALVPSAKCQTELLYTINALRKKKAQIVIACQEAPTQISG
                     LSSGLRGKLDSGLTVDIGIPDDQTRIAILESKAKERGIPLNNELANFIVKHIKGGIGR
                     MEGVLMRLGVHASLLNEELTIELARYSLKDWLDDSAKKTNFEQHSHGKSTVTIENKIL
                     QHICIMFQISEERLHSYRRDRKHIKARQAAVFLLKKLTSLSLSEIGKIIGRDHSTVHA
                     TLKKVDERMLKDDFFQKQMQTFLQEFEEKPVSRRTLEQKRSYRN"
     gene            11494..12000
                     /locus_tag="EYO68_07535"
     CDS             11494..12000
                     /locus_tag="EYO68_07535"
                     /inference="COORDINATES: protein motif:HMM:PF01243.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HugZ family protein"
                     /protein_id="HIC07694.1"
                     /translation="MNQSAVLLTKTKTEYLNFLKKFRSLQLSTIGKNGRPESSYAPFV
                     ADQNNQFYIYISGLARHTKNLLHDSRAGIMFIEPEEQAENIFARKRLTFDCDAEIVVR
                     ESQDWMRIMSLFDDFAGELMQTLRMLADFQLIRLKPNSGLFVKGFGKAYEISGEKMDQ
                     LSHVNPRK"
     gene            complement(12014..13600)
                     /locus_tag="EYO68_07540"
     CDS             complement(12014..13600)
                     /locus_tag="EYO68_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07695.1"
                     /translation="MSSPLLSVKNLSVDFTQGDRVTHAVSHASLELNKGETLALVGES
                     GSGKSVTAHSILRLLPASSSHPDGNIEFEGENLLQMSEAKIRGVRGNRIGMIFQEPMS
                     SLNPLHTIEKQINEVLFVHQGLGRKQARVRTLELLEMVEIQNAKKRLGAFPHELSGGQ
                     RQRVMIAMALANKPDLLIADEPTTALDVTVQAQVLQLLRQLQEQFKMAILLITHDLGI
                     VRKHADRVAVMTQGKIIETGNTEELFLHPQHSYTKILLESEPGGQPVTVTDSATYFVS
                     TRNLKVWFPIQKGVLKRTVDHVKAVDGVSLEIRKGHSLGVVGESGSGKSTLGLALLRL
                     IKSEGEIQFGDVYLDALNQKQITPIRKAMQIVFQDPFGSLSPRMTVEQIVSEGLEIHK
                     LEKEENREEQIVQVLKEVDLDPELRHRYPHEFSGGQRQRIAIARALVLKPSFLVLDEP
                     TSSLDRSVQSQVLELLRNLQKKHNLTYLFISHDLKIIQALCHDIIVMKDGIALEAGPT
                     AKIMNNPQNPYTKALLKAAF"
     gene            complement(13612..14283)
                     /locus_tag="EYO68_07545"
     CDS             complement(13612..14283)
                     /locus_tag="EYO68_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_713536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIC07696.1"
                     /translation="MIDLYTAPTPNGYKVSILLEELKLPYNVVSIELEKKEQKKTEFL
                     KLNPNGRIPTIVDHENNDFTVFESGAILIYLAEKTGKFLSPDPNGRSVTLQWLMFQMG
                     GIGPMQGQAHVFYRYAPEKIEYAIERYQKETLRLYTVLDTQLKEHEYLTGDLSIADIA
                     TWPWVRSYRWAGLDIENLPHLQRWLDVLAERPAFQQGIEVPYKIDRSKMTAEQTQEKG
                     SKILI"
     gene            complement(14276..14890)
                     /locus_tag="EYO68_07550"
     CDS             complement(14276..14890)
                     /locus_tag="EYO68_07550"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIC07697.1"
                     /translation="MKKQYGAILLAGGLSSRMGRDKASLEIGGRTMLERLLLILRPLV
                     AETVVMRAPGQILPRISRELQDWIKLGTDSVAERGPLQGIVDALPLLSSEIDKVFLLT
                     CDLPYLTEEWLQTLRDIMTDEYDLVCTEEENITNPLLALYRRPVLEPAAELLAEGKRR
                     PILLWEGWRMVRLSSPEETPWICQDVNTPDEFKKAQLFFQRKHD"
     gene            complement(15191..15967)
                     /locus_tag="EYO68_07555"
     CDS             complement(15191..15967)
                     /locus_tag="EYO68_07555"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01614.16,HMM:PF09339.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIC07698.1"
                     /translation="MKNIFFDKGSAMHRTLAILEQVANSDQPVTSTEINVNLNLPKAT
                     IHRLCSKLEDEQFLQREIDGKRFLPGLRLRQIALGVFSNEHFRTQRHAVLKLLSEDIG
                     ETCNISVPDGSQMRYMDRAETHWPLRMQLPVGTKVPLHCTSSGKLFLSLMPEEQLNRL
                     IGSLNLEQKTENTLTTRKSLEKALEKIRKKQVGTDNQEFIEGMVCVAVPIYDRRKRLF
                     ATLSIHAPSSRMTLEEISRHVPRLQKASHDLSLITDPDDN"
     gene            16592..18637
                     /locus_tag="EYO68_07560"
     CDS             16592..18637
                     /locus_tag="EYO68_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC07699.1"
                     /translation="MGVGFIAVSILLLTAANTVWAGCPASTVADMKGVKAGQFPQQFE
                     LAEFEKLAGCKMTFQGNPDIARLNGKIRGNPSSVPSVANRLPSEPLVYAPYESIGKYG
                     GTLDVLSNATEAGTSDFLSVRHVNLVRYSDDLQTIVPNIAKDWKWNSDFTQLTFYLRK
                     GHRWSDGAPFTAEDVKFWYDHLGLSPLVMEKPKDYLLVAGKRMTVEVVDPQTVVFNLP
                     APKPGLLAHFATSFAQGFQPKHFLAPFHPELSANADKLAQKAGFENGLAVIKAYFGNS
                     DWTDTPSPLLNSPDKVAKLPADVIPTLESHMTITDTTEGRHLVANPYFHIVDTQGNQL
                     PYISEQDEIYANDNEVRILKLVNAEADYKSQSLQLPSAPILLENQQKGDYTIHLRPEI
                     TLSTFAFNVTSADLEKRKVFGDLRFRQAMSVAINRAEINEVAYFGQGTPKQYIGFSPT
                     PSFVDKKWESHMAQFDPGMANRLLDEIGMKDTDGDGSRELPNGNKIVINLQFSTQGVA
                     GQVVELVAQNWSDVGIKTTVKEVTPDEFRSSQSSNALDVTIWRKSQPMAIMLGNNELW
                     VPPFSDYFGIRTGMLWAEYVDSNGSKGVKPPAYVMQMIDDINAFQSAPAGSAESNKLG
                     ARLVKNMVGNLLFIGITQAPAPIYHRNALKNFTEFKTHSYEYYRTYPYRGTQWYLDE"
     gene            18881..>19135
                     /locus_tag="EYO68_07565"
     CDS             18881..>19135
                     /locus_tag="EYO68_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07700.1"
                     /translation="MVRALMSLFTLLAVSLIVFSLMELVPGNCAERYIAFKNTQGQTI
                     TIEDIQAEERRLGLDKPFVIRWGTWVGKVFFKGEFGDSCIL"
BASE COUNT         5310 a         4036 c         3961 g         5828 t
ORIGIN      
        1 tcttagtctt tgtttcagct ttagtcttag tttttgtttc agctttagtc ttagtttttg
       61 tttcagcttt agtcttagtt tttgtttcag ctttagtctt agtctttgtt tcggctttag
      121 attcaacttc agttttactc tcagcaggag catctttaga attattttta gtatcttctg
      181 tattatcagt ctcttcagct tcaactttat ctttgtcttc tgtagtttca gattcttcag
      241 cagttaaggg ggaagaatct tggttatcat cttgattttc cagtgcagtt gcagtttctt
      301 tagcttcgtc tgtgtcactt tcaggcagat cctcggattt gtctatactt ttgagaccat
      361 tttctttagt ttttgcaaat tctttttcca gttgctcatc tatggaaagc tcatcttcct
      421 cagtggcaac aatattgatt ttgtaaccga ttaattttga agccagacgc acattttgac
      481 ctttgtgtcc aattgcaagt gataattgac ttggtgcaac aacaacatca atttgttgtg
      541 cgtcttcgtc aatttcaaga ctgtcaattt cggccggtgc caatgcattg caggtatatt
      601 ggtcaatgtc ttcgctccag cggactatat ctattttttc tccatttaag tcatttacaa
      661 tagcctgcac tcgacttccg cgcatgccaa cacatgagcc gactgggtcg acatcatcat
      721 ctgtagaata aactgaaatt tttgctctgc ggccaggttc tcttgaagca ttgacaattt
      781 tgacgattcc atcataaatt tccgggacct ccatctcaaa cagcttgatc agtagaccag
      841 gatgtgtcct ggaaattgta agttgagaat ctttatgcgg attatcattt acgtccaaca
      901 gcaacactct gacatggtct ccaaaggaaa aattttcacc atatatttgc tctctccgga
      961 acaaataggc ttcgacatta ttttgaaaac tgaaagcaac acgtccgttt ggttcgaccc
     1021 gcaagacagt tccggttaaa acatctccta ctttgtctgt aaagatggaa acaaccattt
     1081 gtcgttctgc ttcctttaaa ctgccaaaaa taatgggtcg tgttgaatga gcaattctct
     1141 gaaactcgcg atcactaatt tcatactcaa cctcatctcc aagtcctgca tcagggtcta
     1201 tttcaaagac ttcatcgaca gaaatttcat tgtgaggatc ttcaacaagt tcaacaacct
     1261 ccttaaaatg gaataagtta atttttccgg tatcttcgtc gatatgtccc tcaatttctc
     1321 tataaggcag tttgcgctga gcagcctgaa taagcgactc ttctaccagt tttatcacaa
     1381 cttcatggtc caaacccttc tctctagaga agtctttaat tatttcaaat aagttttctt
     1441 tcaaaattca cctttttttg tgttatgttt tatttgaagt tctatcaaac attcaaaatt
     1501 tcagttcagg ttcgagatta gcttttttaa ttagggtata tgggattacc aattcagacc
     1561 catctgattc cagttttaag cgtatttcct gttcagtact ttcttctaat tttccaaccg
     1621 cattttttac tccctggatt ttttgctgca gtcgcactct gattcgttct cccgtgaaag
     1681 tcttataatg ttcagcagtc tttagtttac ggaataatcc cggagacgaa acttctatgc
     1741 tgtaagaatc agaaattggg ttttcattgt ccaaaacttc ctgtgcgatt cgatttacgg
     1801 ctacacaatc gtcaatccca attccattaa tactgtcaat aattaattga acagtctggt
     1861 tagatccgtg tttattatat tcaacatcta gaagttcata accttcactt ttgatcggta
     1921 cagtaagcaa atttatcaac ttttctgcaa tctgggtcat caggtctgct tagatttcag
     1981 gatgttgcta aataattgat taaaaactaa cctttgattc tatctgttta aagatgcttt
     2041 gtcgattctt tttttgtgtt gtttaagctt ttcgttctat ggtcaaatat tccaccagtt
     2101 ctccaagcaa agaactagca gggttaacag gtaaattttc caaagctgca gaagccagag
     2161 ttgcctgttc ttgacaaaaa cttgctacag attccagtgc cccagtactt tctgcttgag
     2221 aaataaacca gtcaatgtcg gcatgtgtaa cgtgttctcg agtttgtaat aaaattgtgc
     2281 gtactcgttc ggcatccttt gcagaaagcc gttccagaag ttcaattgaa atcaaggtgc
     2341 gtttcccttc agcaatgtca cctccatact ctttaccata accgcctgca cctgcgctgg
     2401 gagcttcagt ttcacttttt tcagttaagt taagcaaatc atcacgaatc tgaaatccga
     2461 ttccaattgc ttctccaaat ctgccggctg cttccagctc tgttttggaa gcacccgcaa
     2521 tcaaggcacc acatatgcag ggtcctcgac cggaatacca gcctgttttt cggctaatca
     2581 tttcacggta ttcagcacga tcaggaaaag tctggtttgc tacccagcct atatctagtg
     2641 cctgcccctc aaaagtggcc tgcatcactt tgttcaaatg ttgatggacc tctactgcca
     2701 aattactgtc taatttggaa tgattttcta gcaaagtttc atgtaccagt cccagcaaag
     2761 catctcccgc attaagtgcc agggcgattc cgtgttggag atgtaatgtc tgttctcctc
     2821 gacgtaataa agactcgtcc tctatgtcat cgtgaaccag tgcaaaattg tgaaacaatt
     2881 caagtgcaac tgcagaagtc aaggcttttt ccggatcacc tccaaataat tcacaactta
     2941 gtaaaactag tgcaggacga aatcgttttc ctccacgctg cggataatca cgcatcaaat
     3001 cataaagata ttcctttggt tctccatccg gaatgcagcg cagagtctct tctccaatcc
     3061 attcactggc ggcttttaaa taggatttta gttggacatg atcttttgcg aaaattttca
     3121 tgttgtttat tattaaatgg aaattacgtt ttcttaataa acggttatgg agtttagatt
     3181 ttagagcgat tgaagttttg gaatacggaa attattttta attcgctggt tggcatcagc
     3241 aagctcagca actggcaaaa tgacgctcaa acgctctgaa cctggaagga aatcaattct
     3301 atgaaaatta tttattggac ttaaaaaagc tgtgctaaca tcttccaggc ggcgtatttg
     3361 cttgccgttt acctgcaaaa ccactgcatt agaaagtttt tggtaaccga tgttcagcgg
     3421 gtcaggaata acttgtgaca ctaaaacaat ccggggagga tgttcggagg atatttttgt
     3481 tccggtcgaa gatatatcag ttttattatt ttcgtaaatc gatacagctt tgttttgctc
     3541 caaaaacaac ctcagccgca taggagcacg tgtttgccaa tttgcacccc agattctgag
     3601 gtaattcaag gtcaattcct gaaataaaaa accaccctct ataacatatc gcggagctgt
     3661 tccaacaatt tttaagggaa ctgatcgact attctcttcg tatgcagaaa cttttgtttg
     3721 caaaacaaca ggcttaccct cacggattac ttccagtttt actgaatctc ccactttcaa
     3781 atgagctaaa aaaagcaaat caaacaagga aataccccaa tccggatgct caactttacc
     3841 atcgtgtgaa agcgtccatt ttcccagttt ggtcaaataa tcaccttgca tcaaaacatc
     3901 agaacctgtg ccgtaaggca aaaccctgct gatccagatt ccgggttttt ccggaggaat
     3961 ttgaaaatga ttgtaagtac tgctttgcgg taatctgctc catataaaac cacgatgtgc
     4021 taagctccgg taaggattac tgcctgcgtg cttcaaaaac tcaagcagca ttgcagcagg
     4081 cattgcctta gcttctccag tacctgcatc caggatcatt cctgcagttt cattatcctg
     4141 cagaagcggg tatccctgac cgctttggga aattccgttc agccttaaaa gaggaaacca
     4201 ggcattgctt acttcccggt gtcccactgc cattctgtct attgttccgg actccattgt
     4261 ccactcatct tgagatttta ttttcaggga ataaatatct tttgcctcat gatcagtaga
     4321 tgattttaca ggagtccatt tcacaggtct taggttccgc caaaattttg aattattgat
     4381 ttgaagtaaa gccaaatttg cagcatagtc catcagcaca agcttggctt gaaaggggct
     4441 gcgaatgtcc ttacgtgtca cctcaatcag agttgcatcg gaaacaattt ctgctgttgt
     4501 cagcaggtac aggttattgt tgtttccatt ttcagatact gaaattctca cagaaacggg
     4561 taggcgtaca accagggcca ttccggattg tgtccggatt tcgcgttttt cccacggagt
     4621 gcgccagtca aaagactgct gataagattt caggagaacg gcctgttgaa accatggttt
     4681 tatttccgga ggatcagcct gtaatactga agtaaaaaga aaaaccgtag cagttatggg
     4741 ccatttcaaa agggtataaa gaaaattatt ttgtttcact gagaatcctt gtttttgtcg
     4801 gcataaatac gggaaccgtc aataattccg taacgtttga gaactgaatt atgctgctca
     4861 agactttctt taaaattcag aactaaaggg attttatgcc attcgcttcc aaagacaaca
     4921 ttggttgttt ctggaggcat attctttagc atcttctcca aatgtgctag cgatcgaact
     4981 ggtttaccgt tcagactact tactataaaa ttgtgtaaat tggtgtagcc gctattgact
     5041 ggagaaggca gaacacgtgt aacaatgact acttgttcgt gacgggttcg cggtcgttct
     5101 atttcacgat accagtgttc ataagccagc ggtggggtta tgttccccgg agaatggatg
     5161 taatttcgat tcagggcaat aaaaaccagc ccgccaaaaa taacgtattc tggaagctca
     5221 tcatagctgt ttcgcatttc tgagccaaac ggcgctgcct tgagtgtgag tgtcaggttt
     5281 ataaatttgc cttttcgcca aacctgcagt tgaagggaat cgccgatttg tcggttttct
     5341 gcttcaataa aaaatgtaac ttgctgctca ctgtaacgca ccattcctgc tgcgtcaatt
     5401 tgcaaatcct caattttcgt cagtacatct ccgattaaaa gaacaccgtc tgcagatgaa
     5461 tgtggaacta cccgttctac aagtattcct ccggtatctt ctggaagacc caaatattga
     5521 cgatagtgct gattgataag ggaactggtt tctattccaa tttcaggtac tccatcatat
     5581 cgtccatctt taatatctgc cagaaaacga tttataagtg gaacaggaat aaaataacca
     5641 acatcgttta gtctcaaatt ggattgaaaa gcaactccga ttgcttgtcc ggattgtgtt
     5701 actggtccgc cgctgtttcc gggattgatt gcagaatctg tttgaattag caggtgtgaa
     5761 tcaataccag gatgcacata agttccaaat tcgatacgtg agacaacacc ttcggtgcgt
     5821 gatagttcct ttccgcccac aggatatcca taggcccgga ccctgctgcg taactccgga
     5881 ataccaccta actccagcgg tttcaaatcc tgataaaact tgggatcacg tactttaagt
     5941 aaagccaaat ctgagtcgtg agcaattgcc agcacgtctg ctagaaacgg ttccggactt
     6001 gaaattttat tcaccagcag cattcgagaa tcacttacca cgtgtgcatt tgtcaaaatc
     6061 caattgtcat gaattaaaaa tccacttcct tgtccttggc tcgtgggctt cattttccat
     6121 ggagatttcc agtccggact ttgctgataa tttgtgattc ggaatacgga ttgttcgatt
     6181 gaattttgtg aagcaggcac agacgagacc aaagctaacc aaacacattc tataagaaga
     6241 ataatccaaa aatattttcg gaattgtgga ttgtctttta acaaaaactc acaacgaaag
     6301 ccattcatgg atagaataaa gtgtagggtt gttgagagcg gattacgttc tgctcttcaa
     6361 ataaataagg tccgtcaact ggttttacaa caactgtatg agtgataaga tttttatcag
     6421 tagtccaata atgtgcgtga taggctggtg cttccaaaac aaacttggat tcacgtttca
     6481 gtttcaaatc gagtaccaac tgcatcccca tacttggagc tgtgctgatt acagtatcgt
     6541 tccaactggt attgatcggc acatgaatat gtccgcaaat tattttttca acatgatgat
     6601 gcttgctgat aaccgagcct aaaagttctt tcccgataaa accgtccaca tcagtttcaa
     6661 ggacatcgca ttttatcggc gggtggtgca taaatatcat tgtcggctga gtaggttttt
     6721 cgtttaatcg ggcatcgagc caactcgcct gtgcttcggt aatttcacca cccggacttc
     6781 ctggtaaggt gctgtccaaa gcgattaaac gaagatctga atcttcaatt acatagtcaa
     6841 ttttcccctc agattcaaca ggacacgctt gttttccaaa agttgatcga agtatgtctc
     6901 ggtcgtcgtg attgccggga atgacataaa agggcatctc gaaacggttt agcaaattgg
     6961 cggcatgatt gaactcttct gcttgccccg tactggtaat atcacctgtg accagaacca
     7021 ggtgcggttt tggcagatgt tgatttatgt ggtcgataca gagtactaaa ttctctgcca
     7081 tcggcgcaat cccgtatgtt tttgtgtttt ttcggggtat atgaagatca ctgatttgag
     7141 cgatgagcat aactaattta ttataccaaa aaaattaaaa tcccagttcc cagcacaatc
     7201 ctgtaaatta caaaaaccaa agtgccgtga ttacggagaa agcgcagcaa taactctatc
     7261 gaaaggtagc ccacaacaaa tgcagctaga attcccagaa ttaaatttat gtattggtca
     7321 ctgctgattc caatttccat tagttccttc aattcgtata gaccgctgcc aaaaatcgca
     7381 ggcaatccca gcaaaaaaga aaatctcgca gcagcttcac gatttaaacc caccatcaaa
     7441 ccacccatta tggtggatcc ggaacgggaa cagccgggaa tcagagccaa agcctgtgtc
     7501 aggccgatta attgaatttg taaaaaactt aattgtttaa tgtcacggtt gcgagacgaa
     7561 agtctttcgg caagataaag aaaaattgcc agcactatta atgcagttcc gatcacccag
     7621 agttcacgtg cctcgttttt gatgaaatcc ttaaatccca aacctaagac gactactgga
     7681 atagttccgg ctgcaatact ccaggccatt tttgattctg gagtttcaaa caggttccta
     7741 tgccagaggc tttccagtgc cgccgaggac agttggacta catccttacg gaaaaagagc
     7801 aacaccgcaa ccagtgtacc cagttgaata actgcagaaa attctgtccc cggatcactc
     7861 cagccgagta attccggaac aattcttaag tgagctgtgc tgctgatcgg taaaaattcg
     7921 ctcaatccct gaacaatgcc cagcactatg gcctgtatcc aatccattgg tttttttatg
     7981 tctgaaaata atgattctgt acaaagaaat ctaagtgaaa atttgcattt cctatctaca
     8041 tgtaaaattg agagaattca ggatttatcc ttttgtacat ttaaggttaa ataaatatcc
     8101 gacaattcag aaaaatgtaa tcatctcaga ttaattatca tcagaactga atgaataatc
     8161 aatctggaag ctgatttaag cttcagtctg gtttttttca gtgaggagtt ttagtgtttc
     8221 ttcaagtacc aactcaggct caattagttt caggcagtca tagtgagcaa aacgacaagt
     8281 tcttgaaaaa cagggtgagc aggataaatt gcggctaagt acagtcgcat taggattgat
     8341 tggtgcagtc cagtcgggag aggttgagcc aaaaatggca atttgcggac gctgcaatgc
     8401 gctcattaaa tgcatgcttc cagagtcatt tccgaccagt aacttggctt ttgctaaaat
     8461 tgcgagcaat tccgaaaggg aagtttgtcc acaataattt tgaacaaagt ctagtcccag
     8521 agaaatttca gcaccagatt ttacatccgc ttcggtacct aaaatgagaa ttggatctcc
     8581 aaaaacattg tgcagtagtg tagcaagtgt acgaaaatgt tctagaggcc actgttttgc
     8641 cgggccaaaa atagcccccg gagtgaagac aaaaaaagat tcaggaagag aaatttccaa
     8701 ggagccaagt tgtttttcca cccattcatg agttatttct agtcgcggag gatatttttc
     8761 tgtagaaatc cctggtgcca ataaattcag gtactctttg agtaagtgtt gtgagcgtgg
     8821 ggtagcctcc ggcttttttg ccaaactcag cagaaaactt ctgaattctc cggaatagcc
     8881 gatccggtgc ggtattttgc tcttatgtgc catccatgca gctgaaaaac ttggaggcag
     8941 aacataaaaa taatcgtaat tctcagcacg caaagttttt ccaaaatttc ctgcagtttc
     9001 tttttttgga tcaaaaggaa ttatttgtcc acgttgtgga agcggtattt tttcaaatcc
     9061 ggatttaacg attaaatcaa cctgtgattc tggaaaggct tcaagaacag cgcggacaaa
     9121 tccgcttgac ataaccatat cacccagcca gtttggtgtg cggatcagaa tttttactgg
     9181 agcagattca ggcatttagt atattcattc gagaattgat gacttccgtt gctgccgggt
     9241 acagaaataa tagccgtcag gaagaagaga attatagtgt tttaattcaa tctctttgcc
     9301 gtccttctga ttcagccaga ctcgaagatc tctgacaatg aagttggacg tatctatgtt
     9361 tgaacaaacc atgttaactt gtttgcgtaa actgtccgga tgactaatgt aaatcttttt
     9421 ctgcaaactt gtacatccgg aagaagcaaa aattattaga acctgcaatc tgaaaagaat
     9481 caggattgat ctaagactca ttaaacaatc agactgaatg tttgttccca gtggaaatag
     9541 tgtctgcaga taaatttagc tgaaattgta tggaaaggca attatagtag acagaggaga
     9601 aaaatgcttt tgctgaatta attgatccgg gactcaatta cggtagctgc gtttttgttc
     9661 aagggtccgc cttgaaacag gtttttcttc aaactcctgc aggaaagttt gcatttgttt
     9721 ttgaaagaaa tcatctttta acattctctc gtcgactttt ttcaaggtag catggactgt
     9781 tgaatggtca cgacctatga ttttgccgat ttcactcagg gaaagtgagg taagtttttt
     9841 tagcaaaaat acggctgcct gtcttgcttt gatgtgtttt ctgtcacgac gatatgaatg
     9901 aagtctttct tcagaaattt gaaacattat gcagatatgc tgcaggattt tgttttctat
     9961 agtaactgtg gatttaccat gtgagtgctg ctcaaagttg gttttttttg cagaatcatc
    10021 aagccagtct ttcagagaat aacgagccaa ttctatagta agctcttcat tcagcaatga
    10081 agcatgtacg ccgagacgca tcagaactcc ttccatgcgt cctattcctc ccttaatatg
    10141 ctttacgata aaatttgcca gctcgttgtt cagtggaatt ccacgttctt ttgctttgct
    10201 ctcaagaatt gcaatccggg tttgatcatc gggaattccg atatcaaccg tcagccctga
    10261 atccagtttt ccccgtagtc cggaacttag tccggaaatc tgtgttggcg cttcctgaca
    10321 ggctataaca atttgcgctt ttttcttgcg taacgcatta attgtgtaga gcagttccgt
    10381 ttgacacttt gcagatggaa ccagagcctg caaatcatcc agtagaaaag catcagcctt
    10441 tcggtaacgg tcacggaatt ctttcatttt acttaatctg agatgattaa caaaatcatt
    10501 caaaaaatct tctgcagaaa cctgaactct ttgttttttg ggattgcttt gctggagttc
    10561 attgctgatt ccttcaagca agtgcgtttt tcctgatccg gactcaccat aaataacaaa
    10621 aggattaaag gctatacccg gcatttcaac gactgcttta caagccctgc ttgccaatag
    10681 gtttcgttta cccgggataa aggaatccag agaatatgag ctggtacttc ctgcagcatg
    10741 ttctgttgca aagtctgact tggaattttt tatttccaga tcgttatttt tgtttgcaat
    10801 ttgatcttga ttaagatcaa attcagtttg tacggatttc ttacgacctg tacaactgaa
    10861 tattttgtat tcgaattttt tctctgaaaa tggggctttt tcagggaaaa gttcgttgag
    10921 gacttctttc aggagagatt cgtgattagt cttgatttcg taacggtata ttttgtgttg
    10981 aattccgcct aatacaacct ttgaaggagt tatttttagc aactgaagag aatccaacca
    11041 tgactggctt acatgagaag gcaaagaacg tctaaggcac tctttaaatt ctaatgattg
    11101 attatgactc agctccattt tttcttatat ttcaaatcca tttgctgttt ttctcaagtc
    11161 taaatcagtt aatattttgc ttgagatagg cttaatattt ttcctgtcca tggaatggtt
    11221 tcagtaccag actggcctca ctaatttaat gcaaatcgaa gggtaaaatc aacaaaaaag
    11281 atttctaaaa agatgaataa gttacccata aataactgat aaataagtta aagatttttt
    11341 acaaaaaaat aaaaatatta ttgtatatac taaatcaaag agcaataaag tcgttcaata
    11401 ttaggcatct aaggcataca aaaaaaatga atatgataaa tatttttttc gtgttaatgt
    11461 ttatttcaaa aagcactttt tcaggaatca aaaatgaatc agtcagcagt tttgcttaca
    11521 aagacaaaaa ctgaatattt aaactttttg aaaaaatttc gtagtctgca gttgtccaca
    11581 atcggcaaaa atggacgtcc ggaaagcagc tacgctccct ttgttgcaga tcaaaataat
    11641 cagttttata tttatataag cggactggcc cgtcatacaa aaaatttact tcatgactca
    11701 agggctggaa tcatgtttat agaacctgaa gaacaggctg aaaatatatt tgccagaaag
    11761 cgacttactt ttgattgtga tgcggaaatc gtggtgcgtg aatctcaaga ctggatgagg
    11821 ataatgtctc tttttgacga ttttgccggt gaactgatgc aaacattacg tatgcttgcc
    11881 gattttcagt tgattagact gaagccgaat tcgggtcttt tcgtcaaagg atttggtaaa
    11941 gcctatgaaa tttcgggtga aaagatggat cagttgtcgc atgtaaatcc gcggaaatag
    12001 tgatgttttc cttttaaaaa gctgctttca gtagagcttt agtataagga ttctgaggat
    12061 tattcataat tttcgcggta ggccctgctt ccaaagcaat tccgtctttc atgacgatga
    12121 tatcgtgaca aagtgcctgg attattttta aatcatggct tatgaacaga taagtcaggt
    12181 tatgcttttt ctgcaaatta cgaagcagtt ctaaaacctg agactgtact gagcggtcaa
    12241 gtgaggaggt cggttcatcc agtactaaaa aactgggttt gagtaccagg gcacgggcta
    12301 tggcaatgcg ctggcgctgt cctccggaaa actcgtgagg atagcgatgt cttaattcag
    12361 gatcgagatc gacctctttg agaacctgta caatctgctc ttcacgattt tcttcttttt
    12421 caagtttgtg gatttccagg ccttccgaca ctatttgttc cacagtcatc cgtggactaa
    12481 ggcttccaaa cggatcctga aatacaattt gcattgcctt ccgaatcggt gtgatctgtt
    12541 tttgatttaa agcgtccagg taaacatcac caaattggat ttccccttca ctttttatca
    12601 gacggagcaa agcaagtccg agggtggatt ttccggatcc gctttctcca acaacaccga
    12661 gactgtgtcc tttgcggatt tccaaactga cgccgtcaac tgctttaacg tgatcaacgg
    12721 tccttttcaa aacacctttt tggattggaa accagacttt taaattcctg gttgagacaa
    12781 aatatgtggc agaatccgtg actgttacag gctgcccgcc aggttctgac tccagcagta
    12841 tttttgtata ggagtgctga ggatgcaaaa aaagttcttc tgtatttccg gtctcaatta
    12901 tcttgccctg agtcatcact gcaactctgt cagcatgttt tcgtacaatg ccaagatcat
    12961 gtgtaatcag cagaatggcc atcttaaatt gctcctgaag ttgacgaaga agttgaagaa
    13021 cttgtgcttg tactgtcaca tcgagtgcag tagtcggttc gtctgcaatt agtaaatcag
    13081 gcttgttggc cagtgccatg gcaatcatta cccgctgccg ctgacctccg ctcaattcat
    13141 gcgggaatgc gcctaaacgt ttttttgcgt tttgaatttc caccatttcc agtagttcca
    13201 acgtacgtac gcgcgcctgt tttcgaccta aaccctgatg gacaaacaag acctcgttta
    13261 tttgtttttc aatcgtgtgc agtggattca gagaactcat cggttcctga aaaatcatcc
    13321 cgattcggtt accccggaca ccacgaattt ttgcttcaga catttggagc aaattttccc
    13381 cttcaaattc tatattgccg tcaggatgag atgaggaagc aggcaggagt cgcagaatag
    13441 aatgggcggt aacagatttg ccggatccac tttcacctac taaagccagc gtttcacctt
    13501 tgttcaattc cagcgaggcg tggctcactg catgtgttac tcgatcgcct tgtgtgaaat
    13561 caactgacag attttttaca gaaagaagtg gggaagacat gattagtttt tttatattaa
    13621 aattttactc cctttttcct gagtttgctc tgcagtcatc ttacttcgat ctatcttgta
    13681 tggtacctca attccctgct gaaaagccgg gcgttcggcc aaaacatcga gccagcgttg
    13741 taaatgagga agattttcaa tatccaaacc tgcccagcga taactcctga cccagggcca
    13801 ggtggcgatg tctgcaatgc taagatcacc ggtaagatac tcatgttctt tgagttgtgt
    13861 atccagcaca gtgtaaagtc gcagggtttc tttttggtag cgttcgattg cgtattcaat
    13921 tttttccgga gcatagcgat aaaacacatg agcttgtccc tgcatcggtc ctattccgcc
    13981 catttgaaac atcagccact gtagtgtcac ggagcgtcca ttgggatcag gagacagaaa
    14041 ttttcctgtt ttttctgcca aataaatcaa aatggctccg gactcaaaaa cggtaaaatc
    14101 attgttttca tgatccacaa tggtaggaat cctgccgttt gggttgagct tgagaaactc
    14161 agtctttttt tgttcttttt tttcgagttc aatagagact acattgtagg gcagtttgag
    14221 ttcttcgagt aaaattgaaa ccttgtaacc gtttggagta ggggctgtgt ataaatcaat
    14281 catgctttct ttgaaaaaat aattgtgctt ttttaaattc atccggagtg tttacatcct
    14341 gacaaatcca tggagtttct tcaggagagg aaagacgaac catgcgccat ccttcccaaa
    14401 gcaatattgg gcgtcgtttt ccttcggcaa gtaattctgc ggctggttcg agaacaggac
    14461 gacgataaag agctaaaagc ggattagtga tattttcctc ttctgtgcag acaaggtcat
    14521 actcgtctgt cataatatca cgcaaagttt gaagccactc ttcagttaaa tagggtaaat
    14581 cgcatgtaag caggaacact ttgtcgatct ctgaactcaa aagaggtagg gcatcaacaa
    14641 taccctggag aggtccacgt tctgccactg agtccgtgcc aagcttgatc caatcttgta
    14701 actcccggga aatgcgaggg agaatctgtc ccggtgcacg cattacaact gtttcagcta
    14761 cgagtggacg caagatcaac aatagtcttt caagcattgt ccggcctcct atttcgaggc
    14821 tcgccttatc ccgccccatg cgtgatgaca gtccgccggc cagcagaatc gctccgtatt
    14881 gttttttcat cttagcaaat aatactaaat tattaaagta actcgacagt cactagtttt
    14941 acgtatcatt ccgaactatg tgagggatct tataaaatat cagtatttta gcagatctac
    15001 cccttaagtc gagatgatat gcataagtta atgatagcag tatcttcaga taattcatgt
    15061 ctcacgagta aagtaattaa gattttaggc gcaatagaaa ttagacaaaa tatttgtagc
    15121 ttctgaggca cggagtttta gaagaagtaa tctgttcgaa atgtttttgt gatctatttg
    15181 taaaagaaga ctaattgtca tcgggatcag taatcaagct caagtcatga gaagctttct
    15241 gtaagcgtgg tacatgacga gaaatctctt ccagagtcat tctggaactc ggggcgtgaa
    15301 ttgacaaagt ggcaaataag cgcttccgtc tgtcgtaaat tggtactgcc acacagacca
    15361 ttccttcaat aaattcctga ttatctgttc caacttgttt ttttcgaatt ttttccaaag
    15421 ctttttccaa acttttcctg gttgtcagtg tattctccgt tttttgttct aaatttaaac
    15481 tgccaatcaa gcggttcagt tgttcttcag gcatcaggct gagaaaaagt ttaccactgg
    15541 aggtgcagtg aagcggaacc ttcgtgccca ctggaagctg catgcgtaat ggccagtggg
    15601 tttcagctcg gtccatatat ctcatttgac tcccatctgg aacagaaatg ttacaggttt
    15661 ctccaatatc ctctgagagt agttttaaaa cagcatgtct ttgtgtacga aaatgttcat
    15721 tactgaaaac tcccaaagca atttgtctca gacgaagtcc aggcagaaaa cgtttgccgt
    15781 cgatttcacg ctgtaaaaat tgttcatctt caagtttaga acaaaggcgg tgaatggtag
    15841 ctttcgggag atttaaatta acgttaatct cagtggatgt aacgggttga tcagagtttg
    15901 ccacttgttc caggatggca agagtacggt gcatggcaga acctttatca aaaaaaatat
    15961 ttttcatgtt tttttcttga cctgtttgag ataaagtgag aaaaattatt cggacaatta
    16021 ctaaatggga ctcttagtct cattcctgga ttgtatatta aattgttcgg gttctcaaat
    16081 gcaagatgga atcggtatta aacatggttg ctgaaattat ttgaatgttg tccttttcga
    16141 ttactcatta aatatatggt caaatgaata tccataaggg tgctgtttct ggggtgtggg
    16201 gaagtaattt gttccaggaa acataattac agaagatgct ttataaagga gtttatgcta
    16261 gggttgaaaa tgatataagg gttcacttgt tcattggagt aggattgata attcacggta
    16321 ataaggaaac tgttaaaaaa tacttgaaat attttataat tctctatacc cttgtttgta
    16381 cggaaaaatt aacctgtttt tttggggcaa tccattaaaa agtttgtggt atattttcga
    16441 ttttagtagg ctgaggtctt tctgagttcc agatcagcta gaccccttta atgtctgcac
    16501 ctgaccatgg tggtcagttt gcagtgaaat ctggtttaac caagtaaaag gagaaagtat
    16561 tatgctttac ttgaaaataa aaagaaccat gatgggtgta ggctttatcg ccgtatccat
    16621 cttgctgctg acagcggcaa atacggtctg ggctggatgt ccggcatcga ccgtagcgga
    16681 tatgaaaggt gtcaaagccg gtcaatttcc gcaacagttc gaacttgctg agttcgagaa
    16741 actggcgggc tgcaaaatga ccttccaggg caatccggat attgccagac tcaacgggaa
    16801 aatccgtggg aaccccagtt cagttccttc cgttgcaaat cgactgccgt cagagccgtt
    16861 agtttatgct ccatacgagt cgattgggaa atacggtggc acacttgacg tactgtcgaa
    16921 tgctactgag gcagggactt cagactttct ttctgtgcgt cacgtaaacc tcgtacgtta
    16981 ctccgatgat cttcagacca ttgtaccgaa cattgccaag gactggaagt ggaacagcga
    17041 tttcacgcaa ttgaccttct acctgcgcaa aggccacaga tggtctgatg gtgctccttt
    17101 caccgccgag gacgttaaat tctggtacga tcatctcgga ctttctccct tggttatgga
    17161 gaaaccgaag gattacttgc tggtcgccgg caagcgcatg acggtcgagg tggtcgatcc
    17221 acagacagtg gtcttcaacc tgcccgcccc gaagccggga ctgctggccc actttgcgac
    17281 ttcgttcgcg cagggtttcc agcctaagca cttccttgct ccgttccatc cggaactgag
    17341 tgcgaatgct gacaagctgg cgcaaaaagc cggatttgag aatggtcttg cagtgatcaa
    17401 ggcctacttt ggtaattcag actggaccga cactccgtcg cctctactga attctccgga
    17461 caaggtagca aagcttccgg ctgacgtgat tccgacactg gaaagtcaca tgacaatcac
    17521 tgataccaca gaaggtcggc atctcgttgc taatccatac ttccacatcg ttgacacgca
    17581 gggtaatcag ttgccttaca taagcgagca agatgaaatc tatgccaacg ataacgaggt
    17641 acgtatcctt aaactagtca atgctgaagc ggattacaaa tcgcaatccc ttcaacttcc
    17701 gtctgcacca atcctgcttg aaaatcagca aaaaggagat tacacaatcc atctgcggcc
    17761 ggagatcacg ttatcgacct ttgcgttcaa tgtgacctcg gcagacctgg aaaagcgcaa
    17821 ggtctttggt gatctgcgtt tccgtcaggc catgtcggtc gcgatcaacc gtgcggaaat
    17881 caacgaggtg gcctacttcg gtcaaggaac ccccaagcag tacatcggtt tttcaccgac
    17941 tccgagtttt gtggacaaga aatgggaaag ccacatggcc cagttcgatc cgggcatggc
    18001 taacagacta ctcgatgaaa tcggcatgaa agacaccgat ggcgatggtt cccgcgaact
    18061 tcccaatggc aacaagattg tgatcaacct gcaattctcg actcaaggtg ttgctggcca
    18121 agtggtcgaa cttgtcgccc agaactggtc agacgttggc atcaagacga cggtcaagga
    18181 agtaacaccg gacgaattcc gttcttcaca gtcctcgaac gctcttgatg taaccatctg
    18241 gcgcaagagc cagccgatgg cgatcatgct cggcaacaac gagctttggg tgccaccgtt
    18301 tagcgactac ttcggaatcc gcacaggaat gttgtgggct gaatatgttg attctaacgg
    18361 cagcaagggc gtgaagcctc cagcttatgt gatgcagatg attgatgaca tcaatgcatt
    18421 ccagtctgcc cctgcaggct cagcggagtc gaataaactt ggtgcaagac ttgtcaagaa
    18481 catggtgggc aacctgcttt tcatcggaat cacacaggca ccggcaccaa tttatcaccg
    18541 taatgcgttg aagaacttca ctgagttcaa gacgcattcc tatgagtact accggactta
    18601 tccgtatcgc ggaacccagt ggtatctcga cgagtaataa tctttttact cgattattaa
    18661 attaatgaaa gggctggggg gggaattaag cctccagcct ttttaatgag actttaaatg
    18721 aacaaagtta catccggtgc ctttcttgtt taatgtccat accctatatg tttgactcac
    18781 caagtcaata aaaatatgat cttgtcatat tgaagtcgag tattttcagt ctttaccagg
    18841 tttttaattc accaaaatgc taaagttctt acagttcact atggtgcggg cattgatgtc
    18901 gctgttcacg ctgcttgcag tctcgctgat cgtgttctcg ctgatggaac tggtgccggg
    18961 caactgcgca gaacgctaca ttgccttcaa gaacacccaa ggccaaacga tcacgattga
    19021 agacattcag gcagaggagc gccgcctggg acttgacaaa ccttttgtca tccgttgggg
    19081 aacatgggtc ggcaaggtgt ttttcaaggg agagtttgga gacagttgta tcctg
//
LOCUS       DTVD01000217            5430 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4982_length_5430_cov_481.002419, whole genome
            shotgun sequence.
ACCESSION   DTVD01000217 DTVD01000000
VERSION     DTVD01000217.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_5430_cov_481.002419"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="EYO68_07570"
     CDS             complement(<1..1245)
                     /locus_tag="EYO68_07570"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIC07701.1"
                     /translation="MNNPRPLESVSVLFIHEEQIFAVQRQSYLLAFPGYHAFPGGKID
                     KDESSVEFETEFLCEHAARRMRALQREIIEELGYDLEAGILNGEVLSVSELAEALAPP
                     FAPVRFRTWFYRVDLRERISFKVDSGEFADSFWSSAKDFLETYRTGKILMVPPTRRVL
                     EGLVKNPEAAEFGDLSQDFAEKDRVPCLEMLDGIPILAVRSATLPPATRTNALLLGDA
                     DAAKLLVDPSPNSEEEYRCLLNTIEDKMLDAVFLTHHHPDHHQFSNKLARHLRIPIIL
                     SQDTQQRLTLKYGEDYFENVELRFATENEEVTCWHGSSVRVYEIPGHDAGHLGLAPDS
                     LAWFLVGDLIQGIGTVVIPSPEGDMATYFSTLEKVIALNPEVIIPSHGIPMRSTHRLI
                     ETLKHRRERESQILKLSKSGNSK"
     gene            complement(1245..1979)
                     /locus_tag="EYO68_07575"
     CDS             complement(1245..1979)
                     /locus_tag="EYO68_07575"
                     /inference="COORDINATES: protein motif:HMM:PF12684.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07702.1"
                     /translation="MNKTEAFGIVHNLPFEEYRQLPGLNQSTLKQWLSTASKQNDTYK
                     NLQAMQFGSAGHCLLLEEEKFEELYVRAPNGLRQRGKLGKERWAEFSEQHSRKTVLKA
                     NEWERLQNIRRVFQIHPQIKKLWASGKAEVSLFWQDPEFCLDCKARLDWFDPDSGIIV
                     DLKFTNNVGKAGVQKPIQDYYAVQAAWYSRGVYQLTKKTADFLFVFIEKYAPHSIRVV
                     PVSAGDLKHGLQKIESAVKNISNANK"
     gene            complement(1976..4921)
                     /gene="gcvP"
                     /locus_tag="EYO68_07580"
     CDS             complement(1976..4921)
                     /gene="gcvP"
                     /locus_tag="EYO68_07580"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIC07703.1"
                     /translation="MSSYSTSSVNTQNYKPFSLQELEQRDRFVHRHIGPSENDISEML
                     KSLGMNSLDELINRTVPDSIRMSGELDLPGSRSETEVLNELRSMANRNQVAKSMIGMG
                     YHSTILPGVIQRNLLENPGWYTAYTPYQPEVSQGRLEALLNFQQMIIDLSGMDVANAS
                     LLDEATAAAEAMTFCKRVSRSKSMRFFVADDCHPQTIDVLKTRAEPMGFELIFDNIRE
                     QLENPEADLFGALIQYPGTYGDIHDIEKLIEKWHELGAMVTVASDPLSLVLLKPPGEL
                     GADVVIGSSQRFGVPMGFGGPHAAFFAARDKNIRSIPGRLIGASIDRRGKTALRMALQ
                     TREQHIRREKATSNICTAQVLLGVITGCYAVYHGPDGLKIIAQRIHRLTNILKAGMKQ
                     LGFSADNIYYFDTLTFSLEKKCDSIYERALEEGINLRKTDSEQLGISLDEETTKEDIV
                     TLWRVFAGDKKLPSIDELDSHFMDLSNDSGIPKNLRRTSEILTHPVFHQYHSETAMLR
                     YLRYLQDKDIALDRAMIPLGSCTMKLNATSEMIPISWPEFANIHPFAPPQQTEGYRDL
                     IGELENALIQITGFDAVSMQPNSGAQGEYAGLLTIRSYHKSRGEEQRNVCLIPSSAHG
                     TNPASATLASMKIIIVKCDQDGNIDLENLRILAEKHAENLAALMITYPSTHGVFEDSL
                     IQICDIIHENGGQVYMDGANLNALMGIAQPGKLGPDVLHINLHKTFCIPHGGGGPGMG
                     PIGLKEHLAKFAPNHSVVPIEGLPLENTAVSAAPWGSPGILPISLVFIQLMGGSGLKK
                     SSQVAILSANYLAQRLKEYYPIVYTGKNGLIAHECIIDVRPLKEVSGITEEDIAKRLI
                     DYGFHAPTMSWPVPGTLMIEPTESEPKAEIDRFCEAMISISKETQRVESGAWDKIDNP
                     LKNSPHPSEDLTDPEWSHSYSQEAAVYPLASLRRHKYWPPVARVDNVHGDRNVVCSCP
                     PLESYEEGE"
     gene            complement(5250..5430)
                     /locus_tag="EYO68_07585"
                     /pseudo
     CDS             complement(5250..5430)
                     /locus_tag="EYO68_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399393.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         1460 a         1267 c         1176 g         1527 t
ORIGIN      
        1 tttggaattt ccggatttag ataattttag tatttgtgat tcgcgttcgc gacgatgttt
       61 gagcgtctca atcagacggt gagtacttcg catcggaatt ccgtgagagg gaataattac
      121 ttccggattg agcgcaatta ctttttcgag agtagaaaaa taggttgcca tgtccccttc
      181 aggtgaggga atgacaactg tgccgatacc ctgaatcagg tctcccacga gaaaccaggc
      241 cagtgagtcc ggagccaaac ccaaatgtcc ggcatcatgt ccgggtattt cataaacccg
      301 gactgatgaa ccatgccagc atgttacttc ttcgttttct gtagcaaatc gcaactcaac
      361 attctcgaaa tagtcttctc catatttgag agttaatctc tgctgagtgt cttgactgag
      421 aatgatggga atacgcagat gacgggcgag tttatttgaa aattggtgat gatccggatg
      481 atgatgcgtg agaaaaactg catccagcat tttgtcctcg attgtgttaa gtaaacaccg
      541 gtattcttct tctgaatttg gtgagggatc caccagcaat tttgcggcat ctgcatctcc
      601 cagcagtaaa gcattggtcc tggtagctgg cggaagtgtc gcagatctga ctgccagtat
      661 cggaattcca tccagcattt ccagacaggg tactctgtct ttttcagcaa aatcctgtga
      721 caaatcacca aattctgcag cctccgggtt tttcaccaat ccttctaaaa cccgacgcgt
      781 tggcggaacc atcaagatct ttccagtacg ataagtttcc agaaaatcct ttgcagaact
      841 ccagaaactg tctgcaaatt ctcctgaatc aactttaaaa ctgattcgtt cccgcaagtc
      901 aacccgataa aaccatgtcc tgaaacgaac aggggcaaac ggtggagcca gggcctccgc
      961 aagttcacta actgacaaca cctcaccgtt caaaattcca gcctcaagat catagcccag
     1021 ttcttctata atttcccgct gcagggcccg catacgccgt gcagcgtgtt cacagagaaa
     1081 ttctgtttca aactcaactg aagattcatc tttatcaatt ttaccaccag gaaaagcatg
     1141 atatcccgga aaggctagca ggtacgattg tcgctgaacc gcaaaaatct gctcctcgtg
     1201 tataaataaa actgagaccg actcaagtgg ccgtggattg ttcattattt atttgcgttt
     1261 gaaatatttt ttactgcact ctctattttt tgcagtccat gttttaaatc tcctgcagat
     1321 acaggaacca cacggatgga gtgtggtgca tatttttcga tgaatacgaa caaaaagtct
     1381 gcagtttttt tggtcaattg atacactccg cgactgtacc aggcagcctg aactgcataa
     1441 taatcctgaa ttggtttctg tacacctgct ttgccaacat tatttgtaaa tttaagatct
     1501 acaattattc cggaatcagg atcaaaccag tccagccgtg ctttgcagtc cagacaaaat
     1561 tccggatcct gccaaaacaa tgaaacttca gctttacctg aagcccaaag ttttttaatt
     1621 tgcggatgaa tttgaaagac cctgcggatg ttttgtaagc gctcccactc atttgccttg
     1681 agcactgttt ttctactgtg ctgttcagaa aattctgccc agcgttcttt cccaagtttt
     1741 cctcgctgac gcaatccatt tggagcacgg acataaagct cttcaaactt ttcctcttcc
     1801 agcagcaggc aatgtcctgc actcccaaat tgcattgcct gtagattttt atatgtgtca
     1861 ttttgttttg aagcagttga aagccactgc ttgagagtgg attgattaag gccgggtagt
     1921 tgccggtact cctcaaaggg gagattgtga actattccga aggcttcggt tttgttcact
     1981 ctccttcctc ataactttcc aggggcgggc aggaacaaac cacatttcga tcaccatgta
     2041 cattgtccac acgtgcaaca ggcggccagt atttgtgacg gcgaagagaa gccagtggat
     2101 aaacagcagc ttcttgagaa taggaatgag accattcagg atcggtgaga tcttcggatg
     2161 gatgcggcga atttttaaga ggattatcaa ttttgtccca cgctccggat tcaacccgtt
     2221 gtgtttcttt gctgatcgaa atcatggctt cacaaaaacg atcaatttct gctttgggct
     2281 cgctttcagt gggttcaatc atcaatgttc caggaaccgg ccaggacatg gttggtgcat
     2341 ggaaaccata gtctatcaaa cgtttggcaa tgtcttcttc agtaattccg gaaacctctt
     2401 tcaaaggccg aacgtcgata atgcactcgt gtgcaattaa cccatttttt cctgtataaa
     2461 caatcggata gtattctttt aagcgttgtg ccaaataatt cgcacttaga attgctacct
     2521 gtgaagattt tttcaaacca cttccaccca taagctggat aaaaaccagg gaaattggca
     2581 gtatgcctgg actaccccat ggtgcagctg aaactgccgt gttttccagg gggaggcctt
     2641 ctatcggaac aacactgtga ttcggcgcaa acttagcaag atgctctttc aaaccaatcg
     2701 gacccatccc cggaccgcct ccgccgtgtg gaatacagaa tgttttgtgc aaatttatat
     2761 gcagaacgtc tggtcccaat ttaccgggtt gggcaattcc catcaaagca ttcaggtttg
     2821 cgccatccat ataaacctgg cccccatttt catgaatgat gtcacagatc tgtatcaggc
     2881 tgtcttcaaa tacaccatgc gttgagggat aggtaatcat cagggcagca agattttctg
     2941 cgtgtttttc tgccagtatc cgcaaatttt ccagatctat atttccatcc tgatcacatt
     3001 ttacaataat gattttcatg ctggccagtg ttgcactcgc tggatttgta ccatgcgcag
     3061 agctgggaat caggcagaca ttacgctgtt cttcacctcg tgatttgtga taggagcgga
     3121 tggtcagcaa accggcatat tcaccttgcg caccggaatt cggctgcatt gaaactgcat
     3181 caaatccggt aatttgtatg agagcatttt ccagttcacc gatcaaatcc cggtaaccct
     3241 ctgtctgctg cggtggtgca aaggggtgaa tattcgcaaa ttcaggccag ctgataggta
     3301 tcatttctga agttgcattg agcttcattg tgcaggaacc cagcggaatc atagctcgat
     3361 ccagggcaat atctttatcc tgcaaataac gcagataacg taacatcgca gtttcagaat
     3421 ggtattgatg aaaaacagga tgtgttaaaa tttctgaggt acgacgcagg ttttttggga
     3481 ttccggaatc gttgctgaga tccataaaat gcgaatccag ttcgtcaata gagggcagct
     3541 tcttatcacc agcaaaaacc cgccaaagtg tgacaatatc ttcttttgta gtttcttcat
     3601 ccagactgat tcccaattgt tcggaatccg ttttacgtag atttatccct tcctccaggg
     3661 cacgttcgta aatgctgtcg cacttttttt caagtgaaaa agtgagggta tcaaaataat
     3721 aaatattgtc ggcagaaaat cccagctgct tcatacctgc cttgagaata ttggtaagcc
     3781 tgtgtatgcg ctgggcaatt attttcaagc cgtccggacc atgatataca gcatagcagc
     3841 cggtaattac acccaataaa acttgcgctg tgcaaatgtt gctggtcgct ttttcacggc
     3901 gaatgtgctg ttccctcgtt tgcaaagcca tgcgcaaagc tgtttttccc ctgcggtcta
     3961 tggaggcccc tataagtcgt ccgggaatgc tgcgtatgtt tttatcccgg gctgcaaaga
     4021 aagccgcatg cggcccgcca aatcccattg gaacgccaaa tctttgtgaa ctgcctatga
     4081 ccacatctgc tcctaattct ccgggcggct taagcaaaac aagactcagc ggatccgatg
     4141 caacggttac catggctccc aattcgtgcc atttttcgat cagtttttca atatcatgaa
     4201 tgtcaccata agttcccgga tattgaatta aggcaccaaa taaatctgct tcgggattct
     4261 ccaactgctc ccgaatatta tcaaagatca actcaaaccc catcggttcc gccctggttt
     4321 tcagcacatc tattgtttgc ggatgacaat catcagccac aaaaaagcgc atacttttac
     4381 tgcgggaaac acgcttgcag aaggtcatgg cttctgctgc tgctgtagct tcatccagga
     4441 gcgaagcatt cgcaacatcc attccgctga gatcaataat catttgctga aaattcagca
     4501 gggcttccag gcgaccttga gaaacttcag gctgatatgg agtataggcc gtgtaccatc
     4561 ccggattttc cagcaggtta cgctgtataa ctccgggcag gattgtactg tgatagccca
     4621 tgccgatcat tgattttgca acctgattcc tgtttgccat actgcgaagt tcattgagca
     4681 cttccgtttc ggagcgactt ccgggcaggt caagttcacc agacatgcgg attgaatccg
     4741 gaactgtcct gttaatcagc tcatccagag agttcatacc gaggcttttc agcatttctg
     4801 aaatatcgtt ctccgatgga ccaatgtggc gatgtacaaa acggtcccgc tgttcaagct
     4861 cttgcaggct gaacggttta taattttgag tgtttactga agaggtagaa taagaagaca
     4921 tgctgttgat atgttgaatg gagaaaaata tgatgaaccc gctaaaagtc tatcaaaaag
     4981 gcaatgagaa aacaaattct ttaataaaat ctgatggaaa gctaaactgc ggggaattgt
     5041 cttttttgtc aggaaatgcc tggaaacata tcgcggaaat gtccgcaaaa gtttttttga
     5101 attaacatta aaggtaatat taattacttc tgcatacagt atggcatgtt ctgggcagga
     5161 agtcaaatta gcattaaaaa ctctacccaa agctggaatc cgtagccggc caaaaattct
     5221 gcaccaagtc caaaaatgag atcactgaag aaaacatcac gtcggactaa agccgtgtac
     5281 tcccattgcg gcaggaatag aagttactcc tccggttacc atgaaagcca tcgcggttcc
     5341 cggtaccatt cctttttcga tcaggccggc tactaatgga attaccgcat aaccgttgag
     5401 gtaagccgga atgccgattg tcgggctgaa
//
LOCUS       DTVD01000218            5427 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_4989_length_5427_cov_468.773455, whole genome
            shotgun sequence.
ACCESSION   DTVD01000218 DTVD01000000
VERSION     DTVD01000218.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_5427_cov_468.773455"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            138..584
                     /locus_tag="EYO68_07590"
     CDS             138..584
                     /locus_tag="EYO68_07590"
                     /inference="COORDINATES: protein motif:HMM:PF01424.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07704.1"
                     /translation="MIDDMELSSSDQELMTEINVALISFIKSNETHLQMDPMNSYRRR
                     MVHKIGTEFKLTSESTGEGESRSVRLEKTNASAIPENVNKKRVFDRGIEIFYAKPGAE
                     IVLRNDGSFGISLKERESRALDKRTVEDGEFRIRENKIICKDDSNW"
     gene            578..1033
                     /locus_tag="EYO68_07595"
     CDS             578..1033
                     /locus_tag="EYO68_07595"
                     /inference="COORDINATES: protein motif:HMM:PF00636.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIC07705.1"
                     /translation="MVSKSQIETGLSFAAIEQSSFSLSYLGDAFYELWCRQKILQLLQ
                     NRKQVHQSVVQFVRCQTQARLVHLIMPHLTDEEKNIFRQGRNNKVVSLPKHAFTREYR
                     AATGFECLVGFWYLGKEIKRFEKLMSQTEVQEYLESVLTLCKNSFTNAA"
     gene            1038..1307
                     /locus_tag="EYO68_07600"
     CDS             1038..1307
                     /locus_tag="EYO68_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07706.1"
                     /translation="MIWTFIIFIVAVSVATIAFPLFWNKLQFYQLPENSTQDYSQADS
                     WLSALSDLEVDFSLGRMSKKEYQQQKNFLQRGYLKEEEKSGALEE"
     gene            complement(1265..4543)
                     /gene="carB"
                     /locus_tag="EYO68_07605"
     CDS             complement(1265..4543)
                     /gene="carB"
                     /locus_tag="EYO68_07605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIC07707.1"
                     /translation="MPRRTDIKTVLLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     VLINSNPATIMTDPGLVDATYIEPITLKTLEAVIKQEKPDAILPTMGGQTALNMAIEL
                     HEHGVLKSCGVELIGASIDAINKAENRELFREAMSKIGIGMPSSTIARSIEESLALSQ
                     TIPFPLIIRPSFTLGGSGGGIARNDDELIRIVSHGLTVSPSKEVLVEQSIIGWKEFEL
                     EVMRDHKDNAVIICSIENLDPMGVHTGDSITVAPAQTLSDREYQNLRDMSIKIMREIG
                     VDTGGSNVQFAVNPEDGEVLVIEMNPRVSRSSALASKATGFPIAKIATKLAVGYTLDE
                     IPNDITQKTPASFEPSIDYVVTKIPRFAFEKFPMSKDILGTQMQSVGEVMAIGGTFRE
                     SFQKALRSLETGNSGLNPPDYSDEKMTPQEVLEEQLQHVSSKRIWYVADAMRQGWTNE
                     ALYTQTGIDPWFLWQIRSLVEEEEIIVKTKFSEITPVLLKNWKQHGFSDQRIAELLKV
                     TESRIYKLRYEWNVLPVFKQVDTCAAEFASETPYYYSTYGEENESLPTETAKVLILGS
                     GPNRIGQGIEFDYCCVHASWAVRELGYESIMLNCNPETVSTDYDTSDRLYFEPITFED
                     VMHVIDLERPIGVIVQLGGQTPLKLAKQLNNAGVRLLGTPFESIDRAEDREQFAEMLK
                     KLKLKQPLNGMARSLKEAENIAEKIEYPILVRPSYVIGGRAMMVAETKTQLAECFKEA
                     VQVSPDHPVLIDQFLKGAVEVDMDLLGDGTDSEMGGIMEHVERAGIHSGDSACILPPQ
                     NLTSDVLRRLEEQGKILARELRVKGLMNIQFAVKDDEIFLIEVNPRASRTVPFVSKSI
                     GHPLAKYATWLMLGQKLSDIGFSYETPESVSVKETVFPFISFSGADTELGPEMKSTGE
                     VMGRGENLEDAFMKAQVAAYQSEIKEGHIFIGVTDQDKQGIVPAALEFQKAGRKILAT
                     EGTYKVLQKAGVTNIEKTSLDREDPDNIFDYIDRNEVALIVNTTRPRKRSVDAKHIRR
                     VALMRNLPYFTTVEGTLNIACAIVATDSGKKLNYKSLLISSKTLPEINGHSSNAPDFS
                     SSFK"
     gene            complement(4566..5315)
                     /locus_tag="EYO68_07610"
     CDS             complement(4566..5315)
                     /locus_tag="EYO68_07610"
                     /inference="COORDINATES: protein motif:HMM:PF02698.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIC07708.1"
                     /translation="MERPLKFIVEDIIFGPLLLLILLSCALFLMFRQRNENKLSFRIL
                     LGSWVFLLLGSNTLFFQAISYPLKSMTPKSEKKASDAIVVASAGVHGSGAPTPGSTLR
                     AHTAGKLYLEGQAPLVIITGGVTKPYFPPVNIKGMAIILQGMGVPSHDIIIENRSADT
                     YSNGVETSKILNRLELKSVLLVSHDYHLFRLVSVFKKLGIESYAFSANQSYPKTSIPW
                     WRYFDWANFNRIQTIFHEYFGLLSYKFSGLI"
BASE COUNT         1579 a         1137 c         1098 g         1613 t
ORIGIN      
        1 atcataactc ggatgtcaga agtaaagaaa attattttta aaacctcact ccagcctatg
       61 aaatgcctga aggtgctaat tgcttctgaa gttttcatgc agttaatttt gcatagtaac
      121 cataaaaata gaaaattgtg attgatgata tggaattaag cagttctgac caggaactga
      181 tgacagaaat taatgtggcg cttattagtt tcattaaatc aaatgaaacc catttgcaaa
      241 tggatccaat gaattcgtac cgcagaagaa tggtgcataa aatcgggact gaatttaaac
      301 tgacttctga gtcaactggt gaaggtgaaa gccgttctgt tcgattggaa aaaacaaatg
      361 cctcagctat tccagaaaat gtaaataaaa aaagagtctt tgatcgtggg atagaaatat
      421 tttatgcaaa gcccggtgct gaaattgttt tacgtaatga tggtagtttc ggaatttcac
      481 taaaagagcg cgagagccgt gccctggaca aacgtacagt agaggacggt gaatttcgaa
      541 ttcgggagaa caaaataatc tgcaaggatg acagtaactg gtgagtaaat ctcaaatcga
      601 aacaggtctt tcatttgcgg caattgagca gtcttctttt tcattatcat atctaggaga
      661 cgcattttac gaattgtggt gtcgccaaaa gattttgcaa cttctccaaa atagaaaaca
      721 ggtacatcaa agcgttgttc agtttgtccg ctgccaaacc caggcccgtc tggtacattt
      781 gattatgcca cacttgacgg atgaagaaaa aaacattttc cgtcagggcc gtaacaacaa
      841 agtcgtttcc cttcccaaac atgcatttac aagagaatat cgtgcagcaa ctggatttga
      901 gtgtctggta gggttctggt atttgggcaa ggaaataaag cgttttgaaa aactgatgtc
      961 acaaactgag gtgcaggagt acctggagtc agtcctgacc ttgtgcaaaa actcttttac
     1021 taacgcagct taaagagatg atttggacat ttattatatt tattgtcgca gtatctgtag
     1081 ctactatcgc ttttcctctt ttttggaaca aactgcagtt ctatcaactt ccggaaaatt
     1141 ctacacagga ctacagtcaa gcggattcct ggctctctgc attaagtgat ttagaagttg
     1201 atttttcact tgggcggatg tcaaaaaagg aatatcagca acaaaaaaat tttctgcagc
     1261 gtggttattt gaaggaagaa gaaaagtccg gggcattaga agaatgaccg ttaatttcag
     1321 gaagagtttt gcttgaaatc aaaagtgatt tatagttgag cttttttcca gaatcggttg
     1381 cgacaatagc acacgctata ttcagggtcc cttccacagt ggtgaaataa ggcagatttc
     1441 tcataagagc gactctccgg atgtgttttg catccactga gcgtttccgc ggacgggttg
     1501 tgttgacaat caaggccacc tcgtttcggt caatgtaatc gaaaatatta tccggatctt
     1561 cccggtccag gctcgttttt tctatattag tgacacctgc tttttgaagt actttataag
     1621 ttccttctgt agctaaaatt tttcgtcctg ctttctgaaa ttctagtgcc gcaggaacaa
     1681 ttccctgctt atcctgatcg gtcactccaa taaaaatgtg tccttcctta atttctgatt
     1741 gataagcagc aacctgggct ttcatgaagg catcttccaa gttttctccg cgtcccatta
     1801 cttcacctgt tgatttcatt tcaggaccca gttcagtgtc tgctccagaa aaactgatga
     1861 aaggaaatac tgtttcctta actgaaacag attccggtgt ttcatatgaa aatccgatat
     1921 cactcaattt ttgtcccagc atgagccaag ttgcatattt tgccaaagga tgtccaatag
     1981 atttactgac aaacggaacc gtcctggaag cacgaggatt aacttcaatc agaaaaattt
     2041 catcatcttt aacagcaaat tgtatgttca tcaatccttt cacacggagt tctctggcca
     2101 ggatttttcc ttgttcttcc aatcttcgca acacatccga agtcaggttt tgcggtggca
     2161 gaatacaagc gctgtctcca gaatgaatcc cagcgcgttc cacatgttcc atgatccctc
     2221 ccatttcaga gtcagttccg tcacccagca ggtccatatc aacttcgact gcacctttaa
     2281 gaaactgatc gatcaaaacc ggatgatccg gtgacacttg aacagcttct ttaaaacatt
     2341 ctgcaagctg tgtttttgtt tcagcaacca tcattgcccg accgccaatt acataagagg
     2401 gacgtacaag aatcggatat tcaatttttt cagcaatatt ttctgcttcc tttagtgagc
     2461 gcgccattcc atttagtggc tgtttcaatt tcagtttttt caacatttct gcaaactgtt
     2521 ccctgtcttc agcacggtca atactttcga aaggtgttcc aagcagtctc acaccagcat
     2581 tattgagttg ttttgcgagt ttaagcggag tttgtccacc aagctgtact ataaccccaa
     2641 tcggtcgttc caaatcgatt acgtgcatta catcttcaaa cgtaattggt tcaaaataca
     2701 gacgatccga agtgtcgtaa tcagtgctca ccgtttcagg gttgcagttg agcattattg
     2761 attcataacc aagttctctg actgcccagg aggcatgaac acaacaataa tcaaactcaa
     2821 tgccctgacc gatacgattg ggtcctgaac ccagtatgag aacttttgct gtttcagttg
     2881 gaagcgactc attttcttcg ccataggtgg aataataata aggtgtttct gaggcaaatt
     2941 ctgcagcaca ggtatcaacc tgtttaaaca ccgggagtac attccattca taacgtaact
     3001 tataaattcg gctttccgtt actttcagta attctgcaat gcgttgatct gaaaaaccat
     3061 gttgtttcca attcttcaac aatacaggtg ttatttcaga aaatttggtt ttaacaataa
     3121 tttcctcttc ttcaactaaa ctcctgattt gccacaaaaa ccagggatca attccggttt
     3181 gtgtataaag tgcttcattg gtccaacctt gtctcatggc atcagcaaca taccaaatac
     3241 gtttgctgct gacatgctgg agttgttcct ccaaaacctc ttgcggagtc attttttcat
     3301 ctgaataatc cggaggattg agacccgaat ttcctgtttc tagtgaacga agggcttttt
     3361 gaaaagattc acgaaaagta cctccaatgg ccattacttc acctacagac tgcatctggg
     3421 tacccagaat gtctttcgac atgggaaatt tttcaaaggc aaacctggga attttagtca
     3481 caacataatc aatagacggt tcaaaggagg caggtgtttt ttgtgtgata tcgtttggaa
     3541 tttcgtcaag tgtataaccg acagccaatt ttgtagcaat ttttgcaatg ggaaaacctg
     3601 tagcttttga agcaagagca gaacttcggg aaactctggg gttcatttcg atcacaagga
     3661 cttcaccatc ttccggatta accgcaaact gtacgtttga acctccggta tcgacgccga
     3721 tttcccgcat aatttttatc gacatatccc gcagattttg atattcccgg tcagacagtg
     3781 tttgcgcagg agcaacggta atactgtcac cggtatgtac tcccatcgga tcgagatttt
     3841 caatagaaca aataatgact gcgttatctt tatgatcgcg cataacttcc agctcaaatt
     3901 ctttccagcc tattattgac tgctcaacta aaacctcttt agatggagaa acagtcaaac
     3961 catgggagac aatacggatt aattcatcgt catttctggc tattccacct ccggagcctc
     4021 ctaaagtaaa ggagggtctg atgattaatg gaaacggaat tgtttgagac aaagccaatg
     4081 attcctcaat ggaacgtgca atggtgctgg atggcattcc aattccaatt ttactcattg
     4141 cttcccggaa aagttctctg ttttccgctt tgttaattgc gtcaatggaa gcgccgatca
     4201 gttctacgcc acagcttttc agaacaccat gttcatgaag ttcaattgcc atattgagtg
     4261 cggtctgccc ccccatggtg ggcaatattg catcaggctt ctcctgcttt atgactgctt
     4321 ccagagtctt gagagtaatt ggttcaatat aagttgcatc gaccagcccc ggatcagtca
     4381 tgatggttgc aggattactg ttgatcaaga caacccggta gccctcttct ttgagggctt
     4441 tgcaggcttg agtgccgctg taatcaaatt cacaggcttg tccaattacg atgggacctg
     4501 acccaataag taatacagtc ttaatatcag tacgccgggg cattatgttt gttcagattt
     4561 gtgacttata tcaagcctga gaatttgtaa gagagaagcc caaaatattc gtggaaaata
     4621 gtctggatgc ggttaaaatt agcccagtca aaataccgcc accatggaat cgaagttttg
     4681 ggataggact ggtttgcaga aaatgcatag gactcgatcc ccaatttttt gaagacagaa
     4741 acaagacgaa ataaatggta atcatgagaa accaaaagaa cagattttaa ctccagacga
     4801 ttcaaaattt tggaggtctc gactccatta ctgtacgtat ccgcagaacg attttcaatg
     4861 atgatatcat gcgaaggaac tcccataccc tggagaataa tggccatacc tttaatgttg
     4921 acaggaggaa aatatggttt agttacacca ccggtaataa tcacgagcgg tgcctgtccc
     4981 tccaggtata attttcccgc tgtgtgtgct cttaaggtag agccaggtgt tggtgctccc
     5041 gaaccgtgaa ctccggctga agcaaccacg atagcatcgc tggctttttt ttcacttttt
     5101 ggagtcatac tttttaatgg atatgatata gcttgaaaaa acagtgtatt gcttccaagc
     5161 agtaaaaaca cccagcttcc taaaagaata cggaatgaaa gtttattttc gttcctctgc
     5221 cggaacatca aaaaaagtgc acaggataat aaaatcaaaa gcagcagagg gccgaatata
     5281 atgtcttcaa caataaactt taaaggacgt tccattgatt atttctaatt ctaaaggtat
     5341 ggggctgatc agagttcttg aagggtagtg tatcacttat tcacttggaa ccagaagagt
     5401 ccccaacttt aatgtattaa tattttt
//
LOCUS       DTVD01000219           19063 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_500_length_19063_cov_423.677609, whole genome
            shotgun sequence.
ACCESSION   DTVD01000219 DTVD01000000
VERSION     DTVD01000219.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 19063)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19063)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19063
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_19063_cov_423.677609"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="EYO68_07615"
     CDS             complement(<1..384)
                     /locus_tag="EYO68_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC07709.1"
                     /translation="MEKYDLCIIGGGPSGYAAAMRAVDFNKSVLLVERDRLGGAGIYD
                     GALSSKTFWEISKEFASFSKKMLHYGLAEPDVHFHNVLQEVNDAVFERSDQLEEHLRL
                     VIQLRPEAFRYIKGSAHLISSSEIEL"
     gene            complement(613..2253)
                     /locus_tag="EYO68_07620"
     CDS             complement(613..2253)
                     /locus_tag="EYO68_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIC07710.1"
                     /translation="MKASDLFVKALEEEGVEYIFGIPGEENLDLLNSLKDSSIKLILT
                     RHEQAAGFMAATYGRLTGKSGVALSTLGPGATNLVTAAAYSQLAAMPMVMITGQKPVK
                     HSKQGKFQILDVVDMMRPLTKYTHSIVSGNNIPSSIREAFRISQEERPGAAHLELPED
                     VAGEEAESQPLPCSRARRPVAEGKAIKQAVEMIEKAKNPLLLIGAGSNRKMPARMLRA
                     FVDKTQMPFISTQMGKGVLDERDPLFLGNAALSANDFLHRAIAKADLIINVGHDDIEK
                     PPFFMEQDSFQVIHINFLTAEVDPVYFPQLEVIGDIANSIWQINERIMPQGSWDFSYS
                     LKVKKAVDAHLLEGADDPRFPMLPQRFVADVREAMPSNGIIALDNGVYKIWFARNYKA
                     HEANTVLLDNALATMGAGLPSAMGAHMVFPKRKVMAICGDGGFMMNAQELETAVRLKM
                     NLICLILRDNSYGMIRWKQANLGFEDFGLEYGNPDFVKFAECHGANGYRVEKTEDFVP
                     LLKKCNDTPGVHIVELPVDYSENDRILNHEIKERSKRI"
     gene            complement(2321..3904)
                     /locus_tag="EYO68_07625"
     CDS             complement(2321..3904)
                     /locus_tag="EYO68_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIC07711.1"
                     /translation="MRTFFLQTTVVILAINVVSFFYLPEVLWSMVIFGPLILLGLRDI
                     TQKSHSILRNFPVLGHMRFLLEEIRPEMYQYFVESDTSGRPFSREQRSVVYARAKNTR
                     DTIPFGTVEDVYETGYEWVNHSLSPKKMEHADVRVMIGGPDCKQPYSASLLNISAMSF
                     GALSKNAVIALNQGAKIGGFAHNTGEGGVSPYHLQGGDLIWQVGTGYFGCRDKEGKFC
                     PDTFTDNALREQVKMVEIKLSQGAKPGHGGILPAAKLTAEIAKIRDVPMGKDVDSPPG
                     HTVFTNPLEFIDFVTQLRELSGGKPIGFKLCVGKRREFIAICKAMHETGIAPDFISVD
                     GGEGGTGAAPMEFSNHIGTPLVEGLIFVHNVLVGFDLRKKVKIISSGKMTSGFGLVKR
                     LALGADLCNSARAMMMALGCIQARKCNSNLCPVGVTTQNPSLFGALVPEEKSQRVNNY
                     HRNTVDSACQIMGAMGLEKAEELRPWHLMRRIEAYEIRNFSEIYEYIETGSLLQDTKP
                     ESYARACDAARSDSFTATN"
     gene            complement(4035..4982)
                     /gene="moaA"
                     /locus_tag="EYO68_07630"
     CDS             complement(4035..4982)
                     /gene="moaA"
                     /locus_tag="EYO68_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIC07712.1"
                     /translation="MIDLHGRNINKLRISLTEVCNMACTYCVTSLSDHRRIPDELSTD
                     QMLRLVRMLKEHAGIEKVRLTGGEPLLYPNLIPVISGIREMGIGNIGMTTNGQLLAKK
                     VKALVEAGLQNVNLSLDSLEPEKFKRLGRVGKLHKTLEGIEECMKIGLRVKVNMVVVK
                     GENEDEIVKMLEYGVRQGVEIRYLELMAMGPLYKKDDFKLVTMHEILERISTRYTVQP
                     VSAEVDSTSLRYWVPGGYFGIIPNNSAPFCSTCSRLRLTSNGQLIGCLSNPAPLSIRH
                     LLDHPDPQEPLQKLVQKSISYKQDAAFTGSALVMSRIGG"
     regulatory      complement(4974..5094)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5228..6118
                     /gene="rsmA"
                     /locus_tag="EYO68_07635"
     CDS             5228..6118
                     /gene="rsmA"
                     /locus_tag="EYO68_07635"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HIC07713.1"
                     /translation="MNETTPGLKGNPLNLQDRQHYSCLYQSSINSHQMTKKKWGQNFL
                     IHNNVAETIVEAADVHNGDSILEIGPGRGMLTRSLLERGAQVTAIEVDPALCEKLRKQ
                     FEHEARFTLIDQDIMKVSPEDLAKIVSAPAKVVANLPYNIATPLLLRMLFVRKAWHSL
                     TIMVQLEVAKRICASPDSGKIYGPLSLVGALGFERNIIKIISPDSFRPAPKVDSAIIQ
                     LIPHSSSLTHEEEKQFLKWSHLLFQQRRKTLLNGIRRHFPEWFQNCENSLREKYGLRR
                     PESLDFSEWLMLFSDYLQKI"
     gene            6120..7616
                     /locus_tag="EYO68_07640"
     CDS             6120..7616
                     /locus_tag="EYO68_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HIC07714.1"
                     /translation="MDLVKTLRNAEIRVANYRLFLLQLFMRLCLFVILFTFLLAGVSR
                     GFETVDSLDFSGSGPLFLSDKQIQEDLVQTERLLQDNYVRYPILELKGVSWKSAFKNL
                     EDHLLPDINPVLTHHFQEQLIKTLEFTEDSNIQADLFLKKRHYVQRIEPKVAFYTGIR
                     MAQQRKRFSVLPSLKHPNKIVNHWFIDCKTTMEVFFPILPERQTEKLFMLGQQANHQL
                     QPLDCAFENDSGEKQEIMLPLIFPAAELNRQEMPVFEFKGGRTPYIRWYRDGNPEEIA
                     VKQFHKLARKLQNTPTLIIDVRGNANGSFAFIEKWLKEFTSNHWKNVIVRERQTIPIL
                     KGLLNRVQWNLHHSTARLLVGKDQLEQKLQQLKALIFHFREKEITEKWVETKFIFNGK
                     KDAPRMNTRLIVLANQHCGNGCQFLSALTKQIPDGILIGTNTGPFPKNTSGPIFQLKH
                     SRIMLSFSHRIHLNHQLEPVSPSGYLPDYWLFPPMGLTEILRFVSKTN"
     gene            7747..8739
                     /locus_tag="EYO68_07645"
     CDS             7747..8739
                     /locus_tag="EYO68_07645"
                     /inference="COORDINATES: protein motif:HMM:PRK14014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIC07715.1"
                     /translation="MVKLLTNLLRIPIFVFLFVLNTSFHGTLVSLCAPIKFVIPLSGW
                     RRLWGRVSYWISGGFIITNNFLLNVFYHLEWDIEGLVNLRTDGTYLVISNHLSLLDIP
                     VAQGIFLKQIPFLLFFIKQKLLWIPFLGQALWALEYPTMKRYSKETLKSHPELRGKDL
                     ETAKLSCEKLRGHPVTILNYAEGTRFTPEKHARTKSSFINLLNPRAGGIHTVLSSLGD
                     EITSILNVTLVYPGHSSPNFSDLLFGRVHKIVVRIESLKLGEGVVPSLETIRAKGGSL
                     AVRNCLNELWKAKDKFISKIHSDAELNAETTQPTREPFSTESVGVSSTTPFSSE"
     gene            complement(8667..9002)
                     /locus_tag="EYO68_07650"
     CDS             complement(8667..9002)
                     /locus_tag="EYO68_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIC07716.1"
                     /translation="MEEINFEDFLRVQIRTGTILEATLNTKARNPAYILRIDFGPLGI
                     KTSSAQITENYQPDELVGLQISAVLNFPAKRVAGFKSEVLLLGAYSEEKGVVLLTPTD
                     SVENGSLVG"
     gene            complement(9034..12138)
                     /locus_tag="EYO68_07655"
     CDS             complement(9034..12138)
                     /locus_tag="EYO68_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC07717.1"
                     /translation="MIPRLSPSESTPKFVQNFLEVLSKSEFSGDIHHDNGARIVTATD
                     NSIYQVLPQAVVFPKNSRDIQTVLKIADQQRFSKSIKITARGGGTGTNGQSLTEGIVL
                     DCSRYMNRIIESNFKEGWVCVEPGVVLDQLNEHLAPNKVFFAPDLSPSNRATLGGMVN
                     TDACGKGSRIYGRTSDHVLELTCVLSNGEVLESVPLEPEALSEYKKKSGISANIFKIV
                     DQVVSEKTNLIQEVFPKMSRFMTGYNLAKVYGNSGNNFNLNYLLSGSEGTLAVVSKAK
                     LRLTPLPKHKSLLVVKYETFDDALSDAEMLLKSDPAAIETIDEKILSLAKEDEIYLKI
                     KDFVADEKGKSGRKKRPTRTINLVEFCGSNKNHLEKQVTALCKTIDATNNKSGKATGY
                     YRTVDPQEISNLWSLRKKGVGLLGNTKGERKPLPFVEDTAVPPENLADYIREFRTLLE
                     SYGLEYAMFGHVDVGCLHVRPALDLKNPEEESWIRELSDKVVELVKKYDGVMWGEHGR
                     GFRSEYTAEFFGEELHQDLRRIKEAFDPNNRLNPGKIVTPLSHDDKVVPIEGPLRGHK
                     DRQITPGLLKEYESAINCNGNGACFDYSPENVMCPSSRITRDRLHSPQGRAGMMREWL
                     RLLSVNQQSGVSGKKPLGFLYNLLNKIQNSWRKKQGEYDFSHEVYESMAGCLACKACA
                     TQCPVHVDVPEFRSKFLDLYHGRYLRPLKDYLVGSTESFGRLFSQMPLLANALLSWPL
                     SRMLLKNLIGLRDLPEYSPESVRRLLQKADAPAFDVDELILRTPEELERSVILLQDAF
                     TSFYESQVVMDFYELLKKLDYTVYVAPFSPNGKPLHVKGFLKQFRKVVEKNTNWLSYA
                     ARCGIPLVGLDPSVVLTYRDEYLKILGENKLPFEVLLPQEFLVKKSKKLPGHAVSISK
                     KIQEYQLLGHCTEKTGAQLSQELWQDVFEAFGLSLELIPAGCCGMAGTYGHETDHFEE
                     SRGIYNLSWSKKIPEDPLLRQNILASGFSCRSQVKRFEGFRPLHPLQALLREIISVNS
                     FQNE"
     gene            complement(12170..12982)
                     /locus_tag="EYO68_07660"
     CDS             complement(12170..12982)
                     /locus_tag="EYO68_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIC07718.1"
                     /translation="MPDSLLIKIDASIAEIKLNRPDQANALDEELWFALGNCFNELNE
                     NDAVRVCILHGAGNHFTSGIDLGFLQSIGREVEEYECEGRKRDFLRQKILKLQAAFTQ
                     IEQCSKPVLAAVHGGCIGGGVDLISACDMRYSTSNAVFQIKEIDLGLTADVGTLQRLP
                     HLIPQGIVRELAYTARKFSGKEARELGLVNRCYDDQKTLISAVRKIAEQIASKSPLAL
                     RGVKEMLLHSRDHSVADGLKYVATWNSAMLLSTDLEESVMAMMEKRPPEFKD"
     gene            complement(12984..13937)
                     /locus_tag="EYO68_07665"
     CDS             complement(12984..13937)
                     /locus_tag="EYO68_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase"
                     /protein_id="HIC07719.1"
                     /translation="MKQVPYENNLLDCNKNLQLVKNAVKKNLGVSLPFLSDIAHHMVT
                     PHNNQTRSLVLLQSASLFDDPDDNIIDIGSTLEYLHTASALHRNIKEPEKARRHLQQV
                     QKLWGSEAGILLGDYLLSISFQILVKVGNLDVLECVSLATQNIARGQVLEVSEPPLTA
                     TPKHWRSVTRDKIAGLFGAGAQSAAYWGKSSEATASTLFDFGMHVGMAVQLKTELAAV
                     EDEKLFQQKLKKQDLWSPLCFLLRDCMPPNESKELSDKLLNEFDVPEMFTKLGYLFKK
                     YEVSEKVHSEAEVELREAKECLERLELDSAPLQPLTQYSMI"
     gene            complement(13999..14829)
                     /locus_tag="EYO68_07670"
     CDS             complement(13999..14829)
                     /locus_tag="EYO68_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HIC07720.1"
                     /translation="MLIAGTWGALLLYIISWISGLFSSNLVQPSGSRFQVWTDVTGMR
                     TAWLIHTVTLLGLLWTKAVWPSTFVSDILSVAAWGSMVGVQAFPERLPSLVNTSIVRF
                     FVILLLGLSLIISQRQMPAGSLLLEQTWFYHVLLGSHIIILLAGDIMLGVACVASIIF
                     LYQEHHLKTKLMSSLTLRFPALGTLDRLSWQGILWGFLALSIGLMLGILINSDDHSLL
                     ANLRFGSSVSAWCVFATLLLFRQLHAVRSRWITIWCVVGFVLALMSLIVEMLRLNIAE
                     "
     gene            complement(15045..16334)
                     /locus_tag="EYO68_07675"
     CDS             complement(15045..16334)
                     /locus_tag="EYO68_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIC07721.1"
                     /translation="MSSNQFPENYLWYIFSFLLVSFGTVLFAGCNSSDSPTKNNTGPS
                     VVSGTVFESPLEFDAGENPLGITFADFNDDNKTDYIVASSRKQNGISTTEDGTLTLFQ
                     KNSSSSSRFPFTSSTITPATAEWRQDVISADFNGDNKTDLVVTHTGEDKIKLLRNDGS
                     ANFTDNGSVTVGDVPINLISGDWNSDNQTDLAVVNRDNSSVSILQNSSGVFSVSQTLS
                     VSAHPIQIAGGDWDSDNDTDLAVLLRDNTLVQIWLNGGTGTFSKQTTTYTVGSLPMGM
                     ISGDWNCDGKQDLAVSNSGDNTLSLIYGKGTGEFDTPVTISSGRGPGALAAADFDNDS
                     KMDFVVGHHFLVSTNGVSLITGDFSLTLSDTSSRTGYGSATSFAATTVKDGSAPAAIV
                     ISDVDNDSKLDILITLPVSKKLAVLSGKLYYGNLSCP"
     gene            complement(16324..17319)
                     /locus_tag="EYO68_07680"
     CDS             complement(16324..17319)
                     /locus_tag="EYO68_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07722.1"
                     /translation="MQNVEELQQYVDSLTKELVLTSQTVVAAPGCLPAPCFPEIIMKV
                     LGVVTEHEWEDLTDRARNYLKWITVKAEAIDELAPRPMHTTTRGFELLAIDRDGNSFG
                     EIVERSQELGIPSVLVRFASLIATFLTVRLLRQQMERDREVMPSLTFSQNVDANYLNY
                     PRTLKTLLELFPEYKEMFYFEINEEITEDYLTTIRALADDLGIRLALDDTNKMNINVH
                     HQLLDLADWIKIDFEATANLEEQLLSGYGEKIILHFEEYAHGARSPVIVFEGLSETSP
                     LKVFLEQRWKQIDTVLYFQSRERTPVPPWNKYFGLIQDYHDDKYGLFYKGLIDEQ"
     gene            complement(17483..18907)
                     /gene="dnaB"
                     /locus_tag="EYO68_07685"
     CDS             complement(17483..18907)
                     /gene="dnaB"
                     /locus_tag="EYO68_07685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIC07723.1"
                     /translation="MLSSENKSEKTRVFERTKKMRPQTVDLGELLPHDKMAEQAVLGA
                     IIIQNSILLQILDILTTDDFFSPANNYIFAAMQEMTAQEPPVPIDELTLLSHLKSKHL
                     LEQTGGVDYLNELSQKTPVAENAEFYARIVREKGQLRDIILTAHEVAKRGQEENAENI
                     SDFIAEATDRLQSIDSRTRLKSYSHLKEVLATNFEELEKISESPENVTGVPTGFTELD
                     DLTRGLQKGDLIILAARPSMGKTALAINMALYASGHANIPSLIFSLEMPKEHIAMRLI
                     CSEAKVNNRKLLVGDLEQEDWDKLMEGTTRMMEAPLLIDDRSAVTPNYIRKVIRQAKK
                     EFPSLGLVIVDYVQLMQSNIRNPSREQEISEISRTMKAMAKEFSLPMVVLSQLNRGLE
                     RRTEKRPMMADLRESGALEQDADLIVFIYRDEVYNEDTEDKGIAEISIAKHRNGEIGM
                     KRLAFIGQYTKFANLALGGGGSST"
BASE COUNT         5538 a         4261 c         3729 g         5535 t
ORIGIN      
        1 cagctcaatt tcacttgagc tgattaaatg agcacttcct ttgatgtagc gaaaggcttc
       61 cggacgcagc tgaatgacca gccgcaggtg ttcttcaagt tgatctgaac gctcaaaaac
      121 cgcgtcattc acttcctgca gcacattatg aaaatgcaca tccggttcgg ccaaaccata
      181 gtgaagcatc tttttactga atgaagcaaa ttctttggaa atttcccaaa atgttttaga
      241 ggaaagtgca ccgtcgtata ttccggcacc acccagacga tcccgttcaa caagcaaaac
      301 gcttttatta aaatcaaccg cacgcatggc ggctgcatag ccggatggtc caccgccaat
      361 tatacacaga tcgtactttt ccattgaatg aattttaact taaaatttgt ttgcgatatt
      421 acggcaagaa tgcagccctg caaaacagtt atttcttttg aagtttcctg taccaaggta
      481 caaagatctt gaactgctaa aaattgggga aaataatgga atagagcctc gtagcctcag
      541 tgcctgacta aatttctgtg gaaattcagg cttgatcgat tcaccaacaa ttaactataa
      601 cagagtcaat tattaaattc ttttacttcg ttcttttatt tcgtgattca aaatacgatc
      661 attttccgaa taatcaaccg gcaactcaac aatatgaacc cccggagtat cattgcattt
      721 tttcaaaagt ggaacaaaat cttctgtttt ttccactcga tatccgtttg cgccatgaca
      781 ttctgcaaac ttgacaaaat ccggattgcc gtattccaga ccaaaatctt caaatcccaa
      841 atttgcctgc ttccagcgaa tcattccata agagttatcc cgtaaaatca ggcaaatcaa
      901 attcattttg agacgcaccg ccgtttccag ttcctgggca ttcatcatga aaccaccgtc
      961 tccgcaaatc gccatcactt tgcgttttgg aaaaaccatg tgcgcaccca ttgcagaggg
     1021 taatccggca cccattgtgg ccaatgcgtt gtcgagcaaa acggtgtttg cttcatgagc
     1081 tttgtaatta cgagcaaacc aaattttgta aacaccattg tccagtgcaa tgattccgtt
     1141 ggagggcatc gcttcacgta catctgcaac gaaacgttga ggcagcattg ggaaacgcgg
     1201 atcatctgca ccttcaagga ggtgtgcatc cacagctttt ttgaccttta atgaataaga
     1261 aaaatcccaa gaaccctgcg gcatgattcg ctcattgatt tgccagatgc tgtttgcaat
     1321 atcacctata acttccagct gagggaaata aaccggatct acttctgctg ttaaaaaatt
     1381 aatgtgaata acctgaaatg aatcctgttc catgaaaaaa ggaggttttt caatatcgtc
     1441 gtgtcccacg tttataatca aatccgcttt cgctatggca cggtgcaaaa agtcattggc
     1501 ggacagtgct gcgtttccca aaaataaggg atcacgttcg tctaatacac cctttcccat
     1561 ctgtgtactg ataaaaggca tttgggtttt atccacaaaa gcccgtaaca ttcttgccgg
     1621 catttttcgg ttggacccag ctccaatcaa aagcagcgga ttttttgctt tttcaatcat
     1681 ttccactgcc tgcttgatag ctttcccttc tgctactggg cgacgagcac ggctgcacgg
     1741 cagtggctgg gattcagctt cctctccagc aacatcttcc ggcaactcta aatgtgctgc
     1801 tccaggacgt tcttcctggg aaatcctgaa agcttcccgg atacttgaag gaatgttgtt
     1861 tccgctcaca atcgaatgtg tatatttagt caatgggcgc atcatatcga ctacatcaag
     1921 aatctggaac ttaccctgct tgctgtgttt gacaggtttt tgtcctgtaa tcatcaccat
     1981 cggcatagct gcaagctgag aatatgctgc tgcagtcaca agattcgtgg ctcccggtcc
     2041 aagagtggac agggcgactc ctgacttgcc ggtgagcctt ccgtaagtgg ctgccatgaa
     2101 accggctgct tgttcgtgtc gagtcaggat cagcttgatt gacgagtctt tgagtgaatt
     2161 aagcaaatcc agattctctt cacctggaat gccgaaaata tattccaccc cctcttcttc
     2221 aagcgctttt acaaataaat cagatgcttt catatttttt ttagttatca gttatctatt
     2281 ccaggaatgc atttcacagt tttcagcggg tgcacattcc ttaatttgtt gcagtaaaac
     2341 tatcagaacg tgctgcatca caggcacgtg catagctttc aggcttggtg tcctggagca
     2401 gtgagccagt ctcgatgtat tcgtagattt cagaaaaatt cctgatttca taggcctcga
     2461 ttctgcgcat caagtgccat ggtcttaatt cttctgcttt ttccagaccc attgcgccca
     2521 tgatttgaca ggcactatcc acagtattgc gatgataatt attgacgcgt tgactttttt
     2581 cttccggaac aagtgcacca aacaagctgg gattttgtgt tgtcacacca accggacaaa
     2641 ggtttgagtt acacttacgg gcctgaatac agcccagcgc catcatcatt gctctggctg
     2701 agttacaaag atctgcacct aaagcaagtc gcttgaccag tccaaatccg gaggtcattt
     2761 ttccactgct gataatttta acctttttac gtaaatcaaa gccgaccaaa acgttatgca
     2821 caaaaatcaa gccctcaaca agtggagttc cgatatgatt agaaaattcc atcggagccg
     2881 caccagtccc cccttcacca ccatcaactg agataaagtc cggggcaatt cctgtttcgt
     2941 gcatggcttt gcatatggcg ataaactccc tgcgttttcc cacacacaac ttaaaaccga
     3001 ttggtttgcc tccggataat tcccggagtt gggtaacgaa atctataaat tcaagaggat
     3061 ttgtgaagac agtgtgtccg ggaggagaat ccacatcctt ccccatcggc acatcccgaa
     3121 ttttagcaat ttccgcagtc agttttgcgg caggaagaat tcctccatgt cctggttttg
     3181 caccttgcga caacttaatt tcaaccattt taacttgttc tctcagcgca ttatcggtaa
     3241 aagtatccgg acaaaatttt ccctctttgt cacgacagcc aaaataaccg gttcccactt
     3301 gccagatcaa atcacctccc tggagatgat atgggctgac tcctccttct ccagtgttgt
     3361 gtgcaaaacc gccgatctta gctccttgat tgagcgcaat cactgcgttt ttactcagcg
     3421 cgccaaagct cattgctgat atattgagga ggctggccga atatggctgc ttgcagtcgg
     3481 ggcctccaat catgactcgc acgtccgcat gctccatttt ttttggactg agtgagtggt
     3541 tgacccattc gtagccggtt tcataaacat cttcgactgt accaaaagga atcgtatcac
     3601 gcgtgttttt tgctcgggcg taaacgacgc ttcgctgttc tcgactaaaa ggacgcccgc
     3661 tcgtatctga ttctacaaaa tactggtaca tttccggacg aatctcctca agcaaaaaac
     3721 gcatgtgtcc caacacagga aaatttcgca agatagagtg agatttttga gttatgtcac
     3781 gcagaccgag caaaattagc ggcccgaaaa taaccatcga ccagagaact tcaggaagat
     3841 aaaaaaacga aaccacatta atggccagaa tgaccacagt ggtttgcaga aaaaatgtac
     3901 gcatagggaa agtctcagat gatatgtgga tcaaaactta gcgaattgca actatacttg
     3961 ctctaagtat tacaaaactt ttacaggaaa cctacataaa atgcaagtgt tactccactg
     4021 gaaaatatcg cagattaacc gccgatacga gacatgacaa gagcagaacc cgtgaatgct
     4081 gcatcttgtt tgtaggaaat cgatttttgg acaagctttt ggagaggttc ttgcggatct
     4141 ggatgatcca ataaatgacg aattgagagc ggtgcaggat tactcaggca accgatgagt
     4201 tggccgttgg aagtcaggcg taaacgggaa caagtggaac aaaaaggtgc gctgttgttt
     4261 ggaataattc caaaataccc accagggacc caataacgaa gtgaagttga gtcaacttct
     4321 gctgaaacag gctgcacagt ataacgtgta gaaattcgct ccagaatttc atgcattgtc
     4381 actaatttaa aatcatcttt tttataaagc ggtcccatcg ccatcaactc gaggtagcgt
     4441 atttcaactc cctgtctaac accatattca agcattttaa cgatttcatc ttcattttca
     4501 cccttgacta ctaccatgtt gaccttgacg cgcagtccaa ttttcataca ttcttcaatt
     4561 ccttccagtg ttttatgtag ttttccaact cgacctaaac gtttaaattt ttccggctca
     4621 agggaatcaa gactcaggtt aacgttttgg agacctgctt caacaagagc tttaactttt
     4681 ttggccagca attgaccatt tgtggtcatc ccgatattgc caatacccat ttcacgaata
     4741 ccagaaatca ctggaatcaa gtttgggtaa agaagaggct cgccgccagt cagacgcacc
     4801 ttttcaattc cggcatgttc tttaagcatc cggacaagtc tcaacatttg atctgtactc
     4861 aactcatcag gtatacgcct gtgatcactc aggctggtga cacagtaagt acaggccatg
     4921 ttgcagacct ctgtgagaga aatacgaagt ttattaatat tgcgaccgtg aaggtcaatc
     4981 atggaggttc tcctttccaa agccgggagg cagatcagtt tcccaaccta ccctatcgtt
     5041 cgccaaaccg tggctaccac gttaggtttt ggcgaatcgg agattaataa attattgaat
     5101 tctaaattgt aacgttgaga ctatttcagt tgtactattt agtcaagctc tatttaaaaa
     5161 aatattttga ttattaaact gaaatttgct tttatttggc agctctttta aaatacagtt
     5221 aaataatttg aatgaaacaa ctccaggatt aaaaggtaat ccactcaatt tacaagaccg
     5281 gcaacattat tcctgcttat atcaaagctc aatcaattca catcaaatga ccaaaaaaaa
     5341 atggggccaa aattttctta tccacaataa cgtagcagag acgatagtag aggctgcaga
     5401 tgttcacaat ggagattcga tcctggaaat tggtcccggc cgtggaatgc tgacacgttc
     5461 attgctggaa agaggtgctc aagtcactgc aatcgaagtt gatcctgcat tgtgtgaaaa
     5521 attaagaaaa caatttgaac acgaagcaag atttacttta attgatcagg acataatgaa
     5581 agtatctcct gaagatttgg ctaaaatagt tagtgcacct gcaaaagtag tcgcaaattt
     5641 accatacaat attgccactc cattattatt acggatgctc ttcgttcgaa aagcatggca
     5701 ttcactgaca ataatggtac agttagaggt agcaaaaagg atttgtgcat cacctgactc
     5761 cggaaaaatt tatgggccat tgtctcttgt tggagccctc ggttttgaaa ggaatattat
     5821 aaaaatcatt tctccagact cttttcgtcc tgcaccaaaa gttgattcgg caataatcca
     5881 acttatacct catagttcca gtctcacaca cgaagaagaa aaacagtttc tcaagtggag
     5941 tcatcttctg tttcagcagc gccgtaaaac cttgctgaat gggattcgcc ggcatttccc
     6001 ggaatggttt caaaactgtg aaaattcact tcgtgaaaag tatggtctcc gccgaccgga
     6061 aagcctcgat ttttccgaat ggctgatgct cttcagtgat tatttgcaaa aaatataaaa
     6121 tggatcttgt taagacttta agaaacgctg aaatacgtgt ggcaaattat agactctttt
     6181 tgcttcaact atttatgagg ttatgcctct ttgtcatact ttttactttt ctccttgcag
     6241 gagttagcag ggggtttgaa acggttgatt ctcttgactt ttccggaagc ggccccttgt
     6301 ttctttcaga caaacaaatt caggaagatt tagtgcagac agaacgcctg ctgcaagata
     6361 actatgtccg ttatcccatt cttgagctga aaggtgtcag ctggaaatcg gcctttaaga
     6421 acttagaaga ccacttattg ccagatataa atcctgtact gacacaccat tttcaagaac
     6481 aactgataaa aactttggag tttacagaag attctaatat acaggcagat ctgttcttaa
     6541 aaaaacggca ttacgttcag cgaattgaac caaaagtggc attttacaca ggaatccgta
     6601 tggcacaaca acggaaacgc ttcagtgttt tgccctcttt gaagcaccca aataaaattg
     6661 ttaatcactg gtttatcgac tgcaaaacaa ctatggaggt gttcttcccc attttaccgg
     6721 aaaggcagac tgaaaaactt tttatgttag ggcaacaggc aaatcaccag cttcaacctc
     6781 tggattgtgc atttgaaaat gattccggtg agaaacaaga aatcatgctt ccgctcattt
     6841 ttccagcagc agaactaaat agacaggaaa tgcctgtatt tgaatttaaa ggcgggagga
     6901 caccatacat tcgctggtac agggacggca accccgaaga gattgcagtc aagcaattcc
     6961 ataaacttgc cagaaaacta cagaataccc ccacactgat tattgatgta cggggtaatg
     7021 cgaacggtag ttttgccttt attgaaaagt ggctcaagga atttaccagt aatcattgga
     7081 aaaatgtgat tgtacgggaa cggcaaacaa tccctatttt aaaaggactg ctgaatcgag
     7141 ttcaatggaa tctgcatcat tccactgcac gtttgttggt aggaaaagat caactggaac
     7201 aaaaactcca gcagttaaag gccctcatat ttcatttcag ggaaaaagaa attactgaaa
     7261 aatgggtgga aaccaagttc attttcaacg ggaaaaaaga tgcacccagg atgaacactc
     7321 gcctgatagt tctggccaac cagcattgtg gaaacggctg tcagtttctg tctgccttaa
     7381 ccaaacaaat tccggatgga attctgattg gaacaaatac ggggcccttc cccaaaaata
     7441 cttccggtcc aatttttcag ctgaagcatt cccgcatcat gctgtcattc agccacagga
     7501 tccacttgaa tcatcaactg gaaccagttt ctccttccgg atatcttccg gattactggt
     7561 tgtttccacc aatgggatta acggaaatcc tgcgctttgt cagtaaaaca aactagccct
     7621 cactaacttg tttacaattc agaaacttga ttttctcttg attttcagtc accatcaatc
     7681 ggtgttttgt ttaaaaacaa agtcctgaaa acacaacttt aatataccag ctatttcaat
     7741 ctacagatgg taaagctatt gacaaatttg ttaaggattc caatttttgt ttttttgttc
     7801 gttcttaata catcgttcca cggaactctt gtcagcctat gcgctccaat taaatttgtg
     7861 attccgctgt cgggttggcg tcgtttatgg ggcagagttt cttactggat ttccggaggt
     7921 tttattatca caaacaattt ccttttgaat gtattttatc atcttgaatg ggacattgaa
     7981 ggcctggtta atttgagaac agatggtaca tatctggtta tcagcaatca tctttcacta
     8041 cttgatattc cagttgcaca gggaattttt ttaaaacaaa ttccattttt gctcttcttt
     8101 atcaagcaga aactgttgtg gattcctttc ctgggtcagg cgctctgggc tctggaatat
     8161 ccaactatga agcgttattc caaagaaact ctaaaaagtc atccggaatt gcgtggcaaa
     8221 gatttggaaa ccgctaaact ttcctgtgag aaattacggg gacatccagt gactattttg
     8281 aattatgcag aaggcactcg cttcactccg gaaaaacacg caagaaccaa gtcatccttc
     8341 attaatttac tcaaccctag ggctggtgga atacacacag ttttgagtag tttaggcgac
     8401 gaaattacat ccatactcaa tgtcacactg gtatatcccg gtcactcctc tcctaatttt
     8461 tctgatttgc tgtttggcag ggtccacaaa attgtggtta gaattgaatc cttgaagctt
     8521 ggagaaggag tggtaccgtc actggaaaca atccgggcaa aaggaggttc attggcagtc
     8581 aggaactgcc tgaacgagct ttggaaagct aaagataaat tcatcagcaa aatccatagt
     8641 gatgcagaac taaatgctga aacaactcag cccacaaggg aaccattttc cacagaatca
     8701 gttggcgtca gcagcaccac tcctttttct tcagaatagg ctccaagtag cagcacctca
     8761 gatttaaatc cggcaacacg tttggcagga aaattcaaca ctgctgatat ttgcaggcct
     8821 actaattcat ctggttggta attttcagta atttgcgcag aacttgtttt aatccccagt
     8881 ggaccaaaat ctatgcgcag gatgtaggca ggatttcgtg cctttgtatt gagagttgct
     8941 tccagaatag ttccagtcct gatctgaacc cgtaaaaagt cttcaaagtt aatttcttcc
     9001 atttaatttt ttggatctgg atgtaaaaat aagttattca ttttgaaatg aatttaccga
     9061 gataatttct cttaaaagtg cctgtaacgg atgcagtggt cgaaaacctt caaaacgctt
     9121 cacctggctg cggcaggaaa aaccgctagc caaaatgttc tgtcgtaata atggatcctc
     9181 cggaatcttt ttgctccagc ttaaattgta aattccacgt gattcttcaa aatgatcagt
     9241 ttcgtgtccg tatgttccag ccatgccgca acaacctgcc ggaatcaact ccaatgacaa
     9301 accaaatgcc tcaaaaacat cctgccataa ttcctgtgaa agctgtgctc cagttttttc
     9361 ggtacaatgt cccaacaact gatattcttg tattttttta cttattgaaa ctgcatgtcc
     9421 aggaagtttt tttgattttt ttactaagaa ttcttgcggc agcagaacct caaacggcaa
     9481 cttattttct ccgagaattt tcaaatattc atcgcgatat gttagtacca cacttggatc
     9541 aagaccgaca agaggaattc cgcatcgagc tgcatacgac agccaatttg tgtttttctc
     9601 tacaacttta cggaattgtt tcagaaagcc tttaacatgc agtggttttc cgtttggact
     9661 aaacggggca acgtatactg tatagtctag ttttttcaac agttcataaa aatccataac
     9721 aacttgggat tcataaaacg aagtaaaagc atcctgcagc agaatcacac tacgctccaa
     9781 ttcttccggc gtgcgcaaaa ttaactcgtc cacgtcaaat gcaggggcat cagccttctg
     9841 caggagcctc ctgacagatt ctggactgta ttccggcaaa tctcttaaac caattagatt
     9901 tttcagcaac atacggctca gcggccaact gagtaatgcg tttgccagca acggcatttg
     9961 ggaaaataaa cggccgaagc tctcagttga gccaaccaaa taatctttga gtggacgcaa
    10021 ataacgtcca tgataaaggt caagaaattt tgagcggaat tcaggaacat caacatgcac
    10081 cggacactga gtagcacatg ctttgcaggc taaacaacct gccattgact cataaacttc
    10141 gtgtgaaaaa tcgtactcac cctgtttttt acgccatgaa ttctgaattt tattgagcaa
    10201 attatacaga aaaccgagag gcttttttcc tgaaactcct gactgttgat tcacagaaag
    10261 caagcgtaac cattctcgca tcattcctgc tcttccctgt ggagaatgaa gacgatcacg
    10321 ggtaattctt gaagacggac acatcacgtt ttcaggtgag taatcgaagc atgcaccgtt
    10381 accgttacag ttgattgcag actcatattc ctttagtaat cccggagtaa tttgacggtc
    10441 tttgtgtccg cggaggggcc cctcaatcgg gaccactttg tcatcatgag aaagtggtgt
    10501 tacaattttt cctggattaa gccgattatt tggatcaaac gcttccttga tgcggcgcaa
    10561 atcctgatgg agttcttcgc caaaaaattc tgctgtgtat tcactgcgga aaccgcgtcc
    10621 gtgctcgccc cacataacac catcatattt tttcacaagt tcaactactt tatcagaaag
    10681 ttccctgatc caactttctt cttccggatt cttcaaatcc aaagccggac gcacatgcaa
    10741 acacccaaca tccacatgac caaacatggc atactccaaa ccatacgatt cgagcagtgt
    10801 ccggaactcc cgtatgtaat cagccaggtt ttccggtgga actgcagtat cttccacaaa
    10861 cggaagcggc ttcctctcac cttttgtatt gcctaagaga ccaacacctt tttttcgcaa
    10921 actccataaa ttactaattt cctgtggatc aacggtccgg taataaccag tagcttttcc
    10981 ggatttattg ttcgtggcat caatggtttt gcaaagtgcc gtaacctgtt tttccagatg
    11041 atttttgttg ctgccgcaaa attcaaccag gttaattgtc cgtgttgggc gtttttttct
    11101 tccggacttt cctttttcgt ctgcaacaaa atctttgatt ttgagatata tttcatcttc
    11161 ttttgccaag ctcagtattt tttcgtcaat agtttctatt gcagccggat cagacttaag
    11221 cagcatttct gcatcactaa gtgcatcatc aaaagtttcg tatttaacaa caagtaaact
    11281 tttgtgtttc ggcagcggag tcaaacgaag tttagccttg ctgacaacgg ccaatgttcc
    11341 ttctgaacca gacagcaaat aatttagatt gaaattgttt cccgaatttc cgtaaacttt
    11401 tgccaggttg tatcccgtca taaagcggct catttttgga aaaacttctt gaataagatt
    11461 ggttttctct gaaacaactt gatcaacgat cttaaaaatg tttgcagaaa ttccggactt
    11521 ttttttgtat tcgctcagtg cttccggttc aagcggtacc gattctaaaa cttctccatt
    11581 ggaaagaaca caggttaact ctaaaacatg atcgctggtg cgtccgtaaa tgcgggaacc
    11641 ttttccacaa gcatcggtat tgaccatccc acccaaagta gctctattgc ttggagataa
    11701 atcgggagca aaaaagactt tgtttggagc aagatgttca ttgagctggt ccagcacaac
    11761 cccgggctca acacaaaccc agccttcctt gaaattcgat tcaataattc gattcatgta
    11821 acgtgaacaa tccagcacaa tgccttctgt cagcgactgt ccgttggtac ctgtacctcc
    11881 accgcgggca gtgattttga tacttttact aaaacgttgc tgatctgcaa ttttgagtac
    11941 agtctgaatg tccctggaat tttttggaaa aacaactgcc tgcggtaaga cctgataaat
    12001 gctgttgtct gttgcagtta cgatgcgggc tccgttatcg tgatgaatat ctccggaaaa
    12061 ttcactctta cttaacacct caagaaaatt ttgtacaaat tttggtgtgc tttcagaggg
    12121 actgaggcgg ggaatcatag tgcaataaaa tttaagaagt tttccggtgt taatctttga
    12181 attccggagg ccgtttttcc atcatggcca taaccgattc ttccaaatca gttgaaagca
    12241 gcatggctga gttccatgtt gccacatact tcaggccgtc tgcaacagaa tgatcgcggg
    12301 aatgaagcag catttcctta actccgcgca aggccaaagg ggatttagac gcaatttgct
    12361 cagcaatttt gcggactgcg gaaatcaatg ttttctgatc atcatagcag cgattgacta
    12421 aacctaattc gcgggcttct ttgccggaga acttacgtgc tgtataagct aattcccgga
    12481 ctattccctg tggaattaag tgaggcagac gctgcaaagt accaacatcc gcggtaagtc
    12541 caagatcaat ttctttgatt tggaaaactg cgttggatgt tgaataacgc atatcacaag
    12601 cactgatcaa atcgactcca cctccaatgc atccgccgtg aactgctgcc agcacgggtt
    12661 tactacactg ttctatttga gtaaaggctg cttgaagctt taatattttt tgtcgtaaaa
    12721 aatcacgctt tcgtccttca cattcatatt cctcaacttc ccggccaata gactgtaaaa
    12781 atccaaggtc aattcctgag gtaaaatgat tgccggctcc atggagaatg caaaccctaa
    12841 ctgcgtcgtt ttcgtttaac tcattaaaac agtttccgag cgcaaaccat aattcttcat
    12901 cgagggcatt tgcctggtca ggacggttaa gttttatttc tgcaattgac gcatctattt
    12961 taattaaaag tgaatccggc atatcaaatc atggaatatt gagttaatgg ttgaagtggc
    13021 gcagaatcaa gttccagccg ttcgagacat tcttttgctt ctctcaattc tacctctgct
    13081 tcagagtgaa ctttttctga cacctcatat tttttgaaca gataccccag ctttgtgaac
    13141 atttccggaa catcaaattc attcagcaat ttatcagaca attccttgga ctcatttggc
    13201 ggcatacaat cgcgaagcaa aaagcagagt ggtgaccaca aatcctgttt ttttaatttt
    13261 tgctgaaaaa gcttttcatc ttcaactgcc gccaattcag tttttaactg gactgccatt
    13321 cccacatgca taccaaagtc aaacaatgtt gaagctgttg cttcagaaga cttaccccaa
    13381 taagcagcac tttgcgcacc agcaccaaac aagcctgcta ttttgtctcg tgtaacactg
    13441 cgccagtgtt ttggagttgc agtaagaggt ggttccgaaa cttcaagtac ctgtcctcgt
    13501 gcaatgtttt gggtagccag tgaaacgcat tccaatacat ccaaattacc cacttttacc
    13561 aaaatttgaa aagagatgga cagtagataa tcacccaata aaatgcctgc ttcactaccc
    13621 cataactttt gtacttgctg caggtgacgt ctggcttttt ccggttcctt aatgtttcga
    13681 tgcaaggctg atgcagtatg cagatattcc aaagtactgc cgatgtctat gatattatca
    13741 tccggatcat caaagagact tgcgctttgc aacagaacca gtgatcgggt ttgattgttg
    13801 tgaggagtca ccatgtggtg tgcaatgtct gataaaaaag gaagagatac acccaaattt
    13861 tttttcactg catttttaac caactgtaaa tttttattgc agtccaacaa attattttcg
    13921 taaggaacct gtttcattat taaatgattt ctaatttctg acttaatgtt tgataattaa
    13981 tggtaattaa attgatgttt actctgcaat gttcaatctt aacatttcta caatcaatga
    14041 catcagtgcc aaaacaaatc ctacgacaca ccaaattgtg atccagcgtg aacgaactgc
    14101 atgcagttgg cgaaacagca acagtgttgc aaagacgcac caggcactga ctgatgagcc
    14161 aaaacgcaaa ttggctaaaa gtgaatgatc atctgaatta attaatattc ccagcatgag
    14221 accaatactc aatgcaagaa aaccccacaa aatcccctgc caactgagac ggtccagagt
    14281 gccaagtgca ggaaaacgaa gcgttaatga tgacataagt ttagttttga gatggtgctc
    14341 ctgataaaga aaaatgatgc tggccacaca tgcaactccc aacattatat ctcctgccag
    14401 gagaatgata atatgtgatc caagcaaaac atgataaaac caggtttgct ctaaaagcaa
    14461 cgaacctgcc ggcatttgtc tttgggaaat aatgagagat aaaccaagca gcaggattac
    14521 aaaaaaacgg acaattgaag tgttgaccag acttggcaga cgctctggaa aagcctgtac
    14581 tccaaccata ctcccccatg ctgctacgct gaggatatca ctcacaaaag tagaaggcca
    14641 gactgctttc gtccataaca atcccagtaa agttaccgta tgaattaacc aggccgtgcg
    14701 catacctgtg acatcagtcc atacctgaaa cctggaacct gaaggttgca caaggtttga
    14761 agaaaaaagc cctgaaatcc aactaatgat gtaaagcaac aaggctcccc atgtacctgc
    14821 aatcagcatt tttttatatt ggtgatctaa ttttgctcaa gtgagaactt aactgaattg
    14881 aactgagatc tctctacaaa aaaacgattt ttttcaatat tttgcctgca aacttgacac
    14941 aaaaaccagg ctaaaagaaa tccttaattt tgagcaactt acggagaaag accgttaaga
    15001 gtgtatctct gttacagact ccagtcaatc agattttttg agtttcaagg acaggagaga
    15061 tttccgtaat aaagttttcc ggaaagcaca gctaattttt tactgacagg tagtgtaatt
    15121 aggatgtcaa gttttgaatc attatcgaca tctgaaatta caattgctgc aggtgcagaa
    15181 ccgtctttta ccgtggttgc agcaaaggat gttgctgaac cgtagccagt tctggaagat
    15241 gtgtcgctga gtgtcaatga aaagtcgcca gtgatgagag acactccgtt agtactgacg
    15301 agaaaatgat gtccaaccac gaaatccatt ttactgtcat tatcaaaatc tgctgcagcc
    15361 aatgcacccg gacctcgtcc tgaactgatt gtaacaggtg tatcaaattc tcctgttccc
    15421 tttccataaa tcaaggaaag agtgttgtcg ccagaattgc tcactgccag atcttgtttc
    15481 ccatcacagt tccagtctcc ggatatcatg cccattggca gacttccaac tgtgtaggtg
    15541 gtagtttgtt tgctgaaagt tccagttccg ccgttgagcc agatttgaac aagggtattg
    15601 tctctcaata aaacagccag atcggtatca ttgtcactat cccaatcacc acctgcaatc
    15661 tgaattggat gcgcactgac agacagagtt tgggaaaccg aaaaaacacc actactgttt
    15721 tgcaggattg aaacactgct gttatcgcga tttacaacag ccagatcagt ttgattgtca
    15781 ctgttccaat ctccagaaat gagattgatc ggcacatctc caacagtaac actaccgtta
    15841 tcagtaaaat ttgcactacc gtcatttcgc aataatttaa ttttgtcctc accagtatgc
    15901 gtgaccacca aatcagtttt gttgtctcca ttgaaatctg ctgaaataac atcctgcctc
    15961 cattctgcgg tagcaggagt gatggtactg gatgtaaagg ggaagcggct gctggatgac
    16021 gagttttttt gaaagagagt taaggttccg tcttcagtcg ttgaaatccc gttttgcttc
    16081 cgggaactgg ctacaatata atctgttttg ttgtcatcat tgaaatccgc aaaggtaata
    16141 ccaagaggat tttctcctgc atcaaattcc agcggtgact caaaaacagt tccggaaacg
    16201 actgaaggac cagtgttgtt tttagtcggt gaatcagaag aattgcatcc ggcgaataag
    16261 actgttccaa aacttaccag cagaaacgaa aatatgtacc acaaataatt ctcaggaaat
    16321 tgattactgc tcatcgatca agcctttgta aaagagacca tacttgtcat catgataatc
    16381 ctgaatcaaa ccaaaatatt tattccatgg cggaaccggt gtacgttccc tgctttggaa
    16441 ataaagcact gtgtcgattt gtttccaacg ctgttcaaga aatactttta atggagaagt
    16501 ttctgaaagc ccttcaaaaa caatgaccgg agatcgtgca ccatgtgcat attcttcaaa
    16561 atgcaaaata attttttctc cgtaacctga caacaactgc tcctccaggt ttgcagtcgc
    16621 ttcaaaatca atcttgatcc agtctgctaa atccagcaat tgatgatgta cattaatatt
    16681 cattttattt gtatcatcca gagctaagcg aatacccagg tcatccgcaa gcgcacgaat
    16741 ggttgttaag tagtcttcag tgatttcttc gttgatttca aaataaaaca tttccttata
    16801 ttctggaaac aactccagta gagttttaag agtacgagga taattcagat aatttgcatc
    16861 aacattttgt gagaatgtaa gcgagggcat gacttcacga tcgcgctcca tttgttgacg
    16921 gagaaggcga acggtcagaa aagtagcaat cagtgaagca aagcggacaa gaacagaagg
    16981 tattcccaat tcttgtgaac gctctactat ttcaccaaaa ctatttccat ctctgtcgat
    17041 tgccaacaac tcaaagccgc gcgtggttgt atgcataggc cttggggcaa gttcatcgat
    17101 ggcttctgct ttgaccgtaa tccatttcag gtaattcctg gcacgatctg tcaggtcttc
    17161 ccattcatgt tctgtcacca cccccaaaac tttcataata atttcaggaa aacatggtgc
    17221 cggaagacat cccggagctg ctacaactgt ctgactggtc aataccaact ctttcgtcag
    17281 actgtcaaca tattgctgca gttcttctac gttttgcatc agagtgtttt ttccgggaaa
    17341 cctgtgtctg aagttaaact cacacgatta taattgagta aaattaccca acattatcaa
    17401 gaatctgaaa ttcataaatg ttcatgatta ttaacatcta atccagcagg tctaaattaa
    17461 gaattgattt ttatattacc tgctatgttg acgagcctcc gccacccaaa gccaaattgg
    17521 caaattttgt gtactgtcca ataaatgcca gtcgtttcat accaatttca ccatttcgat
    17581 gttttgcaat gcttatttca gcaataccct tgtcctcggt atcttcgttg tagacctcat
    17641 cacgataaat aaagacaatc aggtctgcat cctgttccaa tgctccagac tctctcaaat
    17701 cggccatcat tggacgcttt tctgtacgtc tttccaagcc tcggttcaac tgggaaagaa
    17761 caaccatcgg cagcgaaaat tctttggcca tggctttcat agttcgtgaa atttctgata
    17821 tttcctgttc ccgcgaagga ttacggatat tactctgcat cagttgcaca taatcaacaa
    17881 taacaagtcc cagtgaagga aattcttttt ttgcctgccg aatcaccttt cttatatagt
    17941 ttggtgttac tgcggacctg tcatcaatca gcaaaggtgc ttccatcata cgagtagtac
    18001 cttccatcag tttatcccag tcctcctgtt caagatcacc aaccagcaat tttctattat
    18061 ttacttttgc ttcactgcaa atcaggcgca tggcaatgtg ttcctttggc atttccaagc
    18121 taaaaataag tgatggaata ttagcatgac ctgaagcata aagtgccata ttaatggcaa
    18181 gagctgtttt acccattgaa ggtcgagctg caagaatgat caaatctccc ttttgcaaac
    18241 cacgcgttaa atcatctaac tcagtaaaac cagttggaac tccagttaca ttttccggag
    18301 attcagaaat tttctccaac tcttcaaaat ttgtggcaag aacttctttg agatgagaat
    18361 aacttttaag tcgagtacgt gaatcgatag actgcaatcg atcggttgcc tctgcaataa
    18421 agtcagaaat gttttccgca ttttcctctt gacctcgttt tgcaacttca tgagcagtca
    18481 ggataatatc tcttaactgc cccttctccc gaacaatacg ggcataaaac tcagcatttt
    18541 cggcaacagg agttttctgt gacagttcat tcaaataatc tactccgcca gtttgttcga
    18601 gtagatgctt ggatttcaaa tgactcagca gagttaattc atctatggga acaggaggtt
    18661 cctgtgcagt catttcctgc atggcagcaa aaatataatt attggcggga gaaaaaaagt
    18721 cgtctgtggt gaggatatct agaatttgca gcaggattga gttctgaata attattgcac
    18781 ccagtacagc ttgctctgcc atcttgtcat gtggaagtaa ttctccaaga tcaacagttt
    18841 gcggacgcat tttttttgta cgctcaaaaa ccctggtttt ttcagattta ttttcagaag
    18901 acagcattga tactcctggt atggagaggg cgatcaaaga tctggaggta gtggggctga
    18961 gacagtaccc aactgataaa ttaaagtgcc aaaggctcaa tgtaggtctg aacctttagc
    19021 tttgaagtta aaatcgtaaa ctactcgtca gtttcgtgtt ctt
//
LOCUS       DTVD01000220            5412 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5011_length_5412_cov_294.423558, whole genome
            shotgun sequence.
ACCESSION   DTVD01000220 DTVD01000000
VERSION     DTVD01000220.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_5412_cov_294.423558"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(97..1188)
                     /locus_tag="EYO68_07690"
     CDS             complement(97..1188)
                     /locus_tag="EYO68_07690"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00989.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIC07724.1"
                     /translation="MISKALISALTDHLPQAVIVIDQKEEVVYCNDSAAQFWQRDSQR
                     LLGKQSRELFHADVMIQEKIRGVLNSGNVFRMGGYVLKTPPLQERGAEIVIAPVRNKT
                     GKVKQAVITLLESTTLQETQAREQEEQLSRALGTLAASLAHEIQNPLSGVRGMLQLLE
                     RDLQKSKISNSSTGMMLAELDRVERLLKQLLLHSHPLPLELTSFDVHELLNTVIRFEQ
                     NTATQIRFIRSFDTSLPDIQADRDKMHQVFLNLIRNAAEASKADASVTVHTRYCGKWE
                     LAGTNLDPERSYTLIAVEDEGSGVSNEQREQLFKPLFSTKKEGHGLGLSISHRLIQAH
                     GGLLRYVQSNSGGSIFQVFLPHHTLDLPV"
     gene            1356..>5412
                     /locus_tag="EYO68_07695"
     CDS             1356..>5412
                     /locus_tag="EYO68_07695"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00580.19,HMM:PF13361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07725.1"
                     /translation="MIQKNNFLSVYIPKQSELFENEPQNSAGKDKQVDVFNGKHDRYA
                     VIEASAGTGKTFALVELVLELVLEQEIPLKSILLVTFTEKATAELRLRLRTKLWELLE
                     ACEKNSASFQTIPEGPFWKINSVRKKQLKAALLDFDSVPVYTIHGFCKRILREFAFEN
                     RQLFEQQLCDTNLLFPEVFRRYMRRELLSRNTPVSQLFALYVKHAEGNLDTLESDINV
                     LLSKDGKFIPLLPQFDVFLKEFKKTWNALTSRDLSLRKQNAAQHPIRTAFESTALSGG
                     SKNKTLRNLELLLEALGEAHSGTTILENLLGVFQIELETVAKPKCRKTLTSGEQWLST
                     EEFPEQERKWIAAVEDCEKLLQSYNFSGSAKKILKAWLTQQVLEDVCRELEQKKLEQG
                     KFDFDDLLRLVEEQVVPPDVKGSAATPLTRAVRKKYACAVVDEFQDTDRRQWSIFRHL
                     FLESPEHRLIVIGDPKQAIYSFRGADIFTYLQARKTIEEKTGRLPFTLKKNYRSSEKL
                     LCGLNQIFSEERWFPEERGIFFQQVGCGKPELELKDQTPDRNAIHLLELLPQFTVSGK
                     KIADQRKAVNPKVSLKILAAFDSLSGKKFFGKEAFLKAVKPVLDFNLSESDQSVILSL
                     FLDDQAENATRRFAEAIALEIKNLLRIGFTKTDRPVWKNEEAERPLREQDICVLFRKS
                     KEGEILGKALRKNGIDFAFYKQKGLFAGREAQEILDLLEAVAHPADHSRTAKVWLTRF
                     FGAKIQDLGQAPTWDSEFLHQLQEWKSLAEARRFRRFFDDVLLQTKLIERELLLSGDE
                     RSVTNYVHLFEVLNKQVLERHLDLFELIQLLRRFIDGKEDPGENENLLRLESERNAVQ
                     LMTMHAAKGLEFPVVFLFGGLTGNKKDTMKFYHDSTEEPVIDLLSSNVPAEHQWQAEA
                     EQQRLLYVAMTRACGRLYLPFAGYLTGNSGNLVCKVSDSYSLINDQLSTIKEQLSAAV
                     DNSPFSSSIVGTSALYKQENVCEIEQEKLRKWKPTHLPVSPIILDDSIEPSKLFNQLR
                     YKKRGFVLSSFSRMNRKKEALQDMEKESALVDDTPVEIRTEIDLGMRRENDETNEIGL
                     PSTAELHSVTTERVDGFQPETNKDPLPGGVQTGNMLHELLELVDFNTIQASNSPQEWL
                     AHPSVYRLLDSFRERYGRSVETLPRVAEIIWSTLRVPVKLGESNDALELADSERFIRE
                     TDFYFPIPFKVKGKMDKVQETQNYSWKVDPKVDGWKVEKGFLRGSIDFLFEHKGLIYL
                     LDWKSNLLSDYHVETLEQEVKQHYELQLQIYTLATSYWFKLDSEEKYNEKFGGVLYIF
                     LRGMPQHKGVYFKRPSWQDLKEYENRLRLENY"
BASE COUNT         1615 a         1063 c         1232 g         1502 t
ORIGIN      
        1 gtttcatttc cagagtaatt acataatgaa aataaggttt gtgatacttt agaccacttt
       61 ttgtttgtca cgattatagc aggatataaa caaattttaa accggaaggt ccaacgtgtg
      121 atgtggtaaa aatacttgaa aaattgaacc gcctgagttg ctttggacgt agcgcaataa
      181 gcctccatga gcctgaataa gccggtgtga aatcgataag cccagaccat gtccttcttt
      241 tttagtgctg aataaaggtt tgaataattg ttcccgctgt tcattagaaa cacctgagcc
      301 ttcgtcttct acggcaatta gagtataact tcgttcagga tccaggttag ttcctgctag
      361 ttcccactta ccgcaataac gcgtatggac agtaacagaa gcatcagcct ttgaagcttc
      421 agcagcattt cgaatcaagt tcaaaaacac ctggtgcatt ttgtcccggt cagcctgtat
      481 gtctggaaga gacgtatcga aactgcggat aaaccgaatt tgagttgctg tattttgctc
      541 aaacctgatt accgtattca gcaattcatg tacgtcaaat gaagtcagtt ccaaaggcag
      601 cggatgagaa tgaagcagca gttgtttcag caaacgttca actcgatcca gttcagccag
      661 catcataccg gtggaggagt tactaatttt tgacttctgc agatcacgtt ccagtaactg
      721 gagcatacct cttactccac ttagcggatt ttgtatttca tgtgccaggg aagcggccaa
      781 tgtccccagc gcacgtgaca actgttcttc ctgctctctg gcttgagtct cttgaagagt
      841 ggtgctttcc agcagtgtta ttactgcctg ttttacctta ccggttttgt ttctaacagg
      901 agcaatcaca atttctgcac cccgctcctg caacggaggt gttttcaaca catatcctcc
      961 catccgaaaa acatttccgg agttgaggac acctcttatt ttttcctgaa tcatgacatc
     1021 tgcatgaaac aactccctgc tttgttttcc gagcaggcgc tgtgagtccc tttgccaaaa
     1081 ttgggcagct gaatcgttac aataaactac ctcttccttt tggtcaatta cgattactgc
     1141 ctgtggcaaa tggtctgtca gagcggaaat gagtgcctta gaaatcatga acggctggtt
     1201 ttcaatagtc gaaatattca aaaaattcaa gcgcccagct taaggtgaaa tatgaggaag
     1261 aacaagttat taacgtgctc cggaaaaatg gagcagttga aatgtgtagc cgggtatatt
     1321 aaaatgcagg tacgtttctc aacctgaaaa atataatgat tcaaaaaaat aatttcctct
     1381 ccgtttacat cccaaaacag tcagaattgt tcgagaatga gccgcaaaat tcggcaggaa
     1441 aggacaagca agttgatgtt tttaacggga aacatgacag gtatgcagtg attgaagcct
     1501 cagctggtac cggcaaaacg tttgcactcg tggaattggt tttggaacta gtgctggagc
     1561 aggaaatacc actcaaaagt attttgctgg tgacctttac tgaaaaagca actgccgagc
     1621 tgcgcttgcg tttgcgtaca aaactatggg aattattgga ggcttgtgaa aaaaacagtg
     1681 cctctttcca aacaattccg gagggtccat tttggaaaat taattcggtc cggaaaaagc
     1741 agttaaaagc tgccttgctg gattttgact cagttccggt ttacacgatt cacggattct
     1801 gcaaacgaat tttacgggaa tttgcttttg aaaaccggca actctttgag cagcaactgt
     1861 gcgacacaaa tctgcttttt ccggaggtgt ttcggcgtta tatgcgcaga gaactgcttt
     1921 cccggaatac tccggtttct caactttttg cactttatgt aaaacacgca gaagggaatc
     1981 ttgacacgct ggaaagtgac attaatgttc tgctttcgaa ggatggtaaa ttcattccac
     2041 tgttgcctca atttgatgta tttcttaaag aattcaaaaa gacctggaac gctttaactt
     2101 cccgcgacct gagtttacgg aagcaaaatg ctgcacaaca tcctatccgt actgcttttg
     2161 aatcaacagc actaagcggg ggttccaaaa ataaaactct ccggaacctt gaactgcttt
     2221 tggaagccct gggagaagcc cattctggta ctacaatcct tgaaaatctg ctcggcgttt
     2281 ttcaaatcga actcgaaaca gttgcgaaac caaaatgcag aaaaactttg acatccggag
     2341 aacagtggct tagcaccgaa gagtttccgg aacaggaacg aaagtggatt gctgcagttg
     2401 aggattgtga aaaattgctg cagagttaca atttttcagg aagtgcaaag aaaatattaa
     2461 aagcatggtt gacacagcag gttcttgagg atgtgtgccg tgaacttgaa caaaaaaaac
     2521 tggagcaggg aaaatttgat tttgacgacc tgctgcgttt ggttgaagaa caggttgttc
     2581 ctccggacgt caaaggctcc gctgcaacac ccctcacccg ggcagttcga aaaaaatatg
     2641 cctgtgcagt tgttgatgaa tttcaggaca cggatcgtcg gcaatggtct atattccgtc
     2701 atttattcct tgaaagtcca gaacatcgat taatcgtaat tggtgatcca aaacaggcta
     2761 tttactcttt ccgtggtgca gatattttca catatctgca ggcacgaaaa acgattgagg
     2821 aaaaaacagg acgcctgccg tttacactta aaaaaaatta ccgctcttcc gaaaaacttc
     2881 tctgcggttt gaatcagatt ttcagcgaag aacgatggtt ccctgaagag cgcggcattt
     2941 tttttcagca ggtcggttgc ggtaaaccgg aacttgaact gaaagaccaa acacctgatc
     3001 gcaacgccat tcatttgctg gaactgttac ctcaatttac agtttccgga aaaaaaattg
     3061 ctgaccagag aaaggcagta aatcctaaag tttctttgaa aatattggct gcattcgatt
     3121 ctttgagcgg taaaaagttt tttggcaaag aagctttttt gaaggcagtt aaacctgtgt
     3181 tggatttcaa tttgagtgaa tccgatcaaa gtgtgatctt gagtttattt ctggacgatc
     3241 aggcagaaaa tgctactcgt cgatttgcag aagcaattgc gcttgaaatt aaaaatcttt
     3301 tgcgaatcgg atttaccaaa actgaccgac cggtctggaa gaatgaagaa gcagaacgac
     3361 ctttgcgtga gcaggatatt tgtgtgcttt tccgcaaaag taaggaaggt gaaatactgg
     3421 gaaaagcttt acgcaaaaac ggaattgact ttgcgtttta caaacagaaa ggtttatttg
     3481 ccggccgtga agctcaggaa atccttgatt tgctggaagc agttgctcat cctgcagatc
     3541 attcgcgtac agctaaagtt tggctgactc gtttttttgg cgcaaaaata caggatcttg
     3601 gacaagcacc tacatgggat tcagaatttt tacatcagct tcaggaatgg aagtcgttgg
     3661 cggaggcccg gaggtttaga aggttttttg acgatgttct gctgcagaca aaattgatcg
     3721 agcgtgaatt gctgctgagt ggagatgaga gaagtgtaac gaattatgtg catttgtttg
     3781 aggtactgaa caagcaggtc ctggagcggc accttgattt gtttgaattg attcagttgc
     3841 tgcggcgttt cattgacgga aaagaagatc ctggcgaaaa cgaaaatttg ctgcgacttg
     3901 aaagtgaacg aaatgcggtt cagctaatga caatgcatgc cgcaaaaggc ctggaatttc
     3961 cagtcgtatt tttatttgga ggactgaccg gaaacaaaaa agacacgatg aagttttatc
     4021 atgattcaac tgaagaacct gtcatagatt tgctcagttc aaatgttccc gctgaacatc
     4081 agtggcaggc ggaagcagaa cagcaacgtc tgctttatgt tgctatgact cgtgcctgcg
     4141 gacgtctcta tttgccattt gcaggatatt taacgggaaa ttcaggaaat cttgtatgca
     4201 aagtcagtga cagttattca ttgattaacg atcaattatc aaccatcaaa gagcaattat
     4261 ctgctgctgt tgataattct cctttcagca gcagtattgt cggcacatct gctttgtaca
     4321 aacaagaaaa cgtatgtgaa attgaacagg aaaagttgcg taagtggaaa cctactcatc
     4381 ttcctgtgag cccaattatt ttggatgatt caattgaacc aagtaaatta ttcaatcaac
     4441 tccgctataa aaaaaggggc tttgttttaa gttccttcag caggatgaat agaaaaaagg
     4501 aagcactgca ggacatggaa aaggagtctg ctttggtaga tgatacgcct gtagaaatac
     4561 gtacagaaat tgatttggga atgcgccggg aaaatgatga aaccaacgaa attggattgc
     4621 cttcgactgc agaactgcac tcggttacca cagaaagagt agatgggttc caaccagaaa
     4681 caaataaaga tccgcttccg gggggagttc aaacaggaaa tatgttgcat gaattactgg
     4741 aacttgtgga ttttaacaca attcaagcaa gtaattctcc ccaggaatgg cttgctcatc
     4801 ctagcgtata tcgtctgctt gattcgttta gggagcgtta tggacgttct gttgaaacat
     4861 tgcctcgagt cgcagaaata atctggagta ctttgcgagt gccggtgaaa ttgggagaat
     4921 ctaatgatgc tttggaattg gctgacagtg aacgttttat aagagaaact gatttttatt
     4981 ttccaattcc tttcaaggta aaaggtaaaa tggacaaggt gcaggagaca caaaactaca
     5041 gctggaaagt cgatccaaaa gtagacggct ggaaagttga aaaaggattt ttgcgaggtt
     5101 caatcgactt tttatttgaa cacaaaggac tgatttattt gctggactgg aaaagtaatc
     5161 tgctgtcaga ttaccatgtg gaaacattgg aacaagaggt caagcagcac tatgaactgc
     5221 aactgcagat ttacactctt gcaacgtcct actggttcaa actcgatagt gaagaaaaat
     5281 ataacgaaaa atttggcggc gtactctaca tctttttacg tggaatgccc cagcacaagg
     5341 gcgtctactt caagcgtccg agctggcagg atttgaagga atacgaaaac aggctgcgct
     5401 tggaaaatta tt
//
LOCUS       DTVD01000221            5405 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5026_length_5405_cov_444.354019, whole genome
            shotgun sequence.
ACCESSION   DTVD01000221 DTVD01000000
VERSION     DTVD01000221.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_5405_cov_444.354019"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="EYO68_07700"
     CDS             <1..1757
                     /locus_tag="EYO68_07700"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07726.1"
                     /translation="KQRGLWVAILLIFVLAMTTWHVWNTESKLDSVWKNWSPAGKSAN
                     FKILKRQKLLIKQGAKTTLLIAATSDLHGNLTSTRLLPRPRPRGLLHLVQKVRQLRAN
                     NPELILLDAGDTIQGDPSSFYFSHIVQEKHRPLPIIEVMNRLKYDAIALGNHDFEPPV
                     SVLKQNIAQSNFSWLAANVRFQNAKQPLFPPYKILIRNGVRVGVLGLITPGVPLWIDP
                     EQRKGLIFDEMLETAKYWVKVLREEENIDFLIGLFHSGDNTRYDQDVASARDLPYPNA
                     AGMIADYLPVFDLIISGHAHRISPKRRTNQLNGHQTPLIAPGTAAEGLSTVLVNFEEQ
                     SGHWKITETVYDYLKAGVSPESQLLNKLEPRLQKVEKYLQEQTSVLFKRFPDKIELYA
                     CGADLSFAAVKKLAPEALSLLPWWWRLEKLPESDLGNSLRREHFFRWLPYDNRIVLVQ
                     LFGRQIEIMLESYRRKQFGWYARSSTILAPGGFTAEVDKSRKGSRLFLTGLDEHAFNK
                     EKMYPVWLTNFHWNGGGGLGAKALLNSGQLVRKESVHLRELIFQHLRSPSVKLPEKCK
                     LFLGNSTRSASHNSEAGQ"
     gene            complement(1811..2182)
                     /locus_tag="EYO68_07705"
     CDS             complement(1811..2182)
                     /locus_tag="EYO68_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07727.1"
                     /translation="MLTGSYKNKFGMLKFARIVFSLGAIVLAGCSSQEVYVYEKLPEA
                     SECRRYTDYWTVSACNRKHQIKMNEKLDSVIRTTNYRLSRKYNQRDKCKERKYRSAPQ
                     GCYISHLPSRANLPTFGKTAD"
     gene            2489..2686
                     /locus_tag="EYO68_07710"
     CDS             2489..2686
                     /locus_tag="EYO68_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07728.1"
                     /translation="MARNPDDWRLDEFHAHHKSGVKIWIGNGVFGYHIEKPEYQEIGL
                     IEKFKLHRQLKLLLENKKTAS"
     gene            complement(2952..4385)
                     /locus_tag="EYO68_07715"
     CDS             complement(2952..4385)
                     /locus_tag="EYO68_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIC07729.1"
                     /translation="MTHESYTKKGLMKKLQQKWWNYKVLFLVLGFFLLVNFVWAAAQN
                     FQKIAKEFQESAISDFIGFSVKGERYPLMHTISELRYRVFEEKLGINKKVETRACSLN
                     RTYGVAVPNDQKKFCQDYDALVELAKTSLIQTALFEKIYLNAEPFLLYEIERQRARES
                     VRIWIRQKMKCLKDKYSCGRDERNMIKRVTKFEPKILKTYLKLLYNLEKKLFPEEQVA
                     TIIKTKDLETLCGFVDQVSQKTARPGMILIDSGNFKMGSSEGLPSEHPVREVKVDEFW
                     IDKCEVTNYQFLLVAAQHPFLRKSTFPRKFHDGNYLLNWPDDLLPETGSELKPVIHVS
                     WYAARHYCNYVGKRLPSEAEWEIAARAGTEGAYSVEGGAEFLEDYAWFRENSGRTIHT
                     TAQKISNPNGLFDIHGNAWEWVYDWFGIYTTDNASNPQGPEIGKYRVLRGGSWSNPPE
                     YLRSAMRRDALPTSTSNNIGFRCAAKK"
     gene            4696..>5405
                     /locus_tag="EYO68_07720"
     CDS             4696..>5405
                     /locus_tag="EYO68_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07730.1"
                     /translation="MFSLNFRKTGWLARYLIFRASTPFTGPEPYLEFTGEDFGEEKFD
                     ELLYLEVEKNGMFFGCPVISRPVQNLANKLNFPKQQGGTILLYLETLFSIALIENESL
                     TSNLQHAATIPYHNRLLKIILLALRYHIPGIFYRIPEDILLTELLAENETLHGALKQF
                     EEELLDSVTLKGYSSLGNRQNNFAFSKLYFFLLWTRAEAKNDKSEPEAFLEMDKQLRE
                     EMILTFAALIWADDYVDST"
BASE COUNT         1583 a         1119 c         1076 g         1627 t
ORIGIN      
        1 caaagcaaag aggactttgg gtggccatcc tgctgatttt tgtactggcg atgacgacct
       61 ggcatgtctg gaacacagaa tcaaagttgg attcagtatg gaaaaactgg agtcctgcag
      121 gaaaatccgc aaatttcaaa atcttaaagc gccagaaatt actgatcaag caaggggcaa
      181 aaacaacatt gttgattgca gcaacttcag acctgcatgg aaacctgact tcgacccgcc
      241 tgctgccacg accacgacca cgaggcttac tgcacctggt tcagaaggtg cgtcaattgc
      301 gggcaaataa tccggaactg attcttttgg acgctggaga cacaattcaa ggcgatccct
      361 ccagttttta tttcagtcat attgttcagg aaaaacatag acctcttcca ataattgagg
      421 tgatgaatcg gctgaaatat gatgccattg ctcttggtaa tcacgatttt gaaccacccg
      481 taagtgtgtt aaaacagaac atcgctcaat caaactttag ctggcttgca gcaaatgtca
      541 gatttcaaaa cgcaaaacag ccactttttc cgccatacaa aattttaatc cggaacggag
      601 tacgtgttgg tgttttaggt ttgatcacac ccggagttcc tctttggatt gatccggaac
      661 agagaaaagg gctgatcttt gatgaaatgc ttgaaaccgc taaatactgg gtaaaggttt
      721 tgcgtgagga agagaacatc gattttttaa ttggattgtt tcacagtgga gacaacacac
      781 gctatgatca ggatgtcgcc tccgcaagag atctgcctta tcccaatgct gccggaatga
      841 ttgccgatta tcttccggta ttcgacctga ttatttccgg tcatgcacac aggatctccc
      901 ctaaacgtcg cacaaatcaa cttaatggac atcagactcc tttaattgcc cccggaacag
      961 cggccgaagg attgagtacg gttctggtga atttcgaaga acaatccgga cactggaaaa
     1021 tcactgaaac ggtttatgat tatttgaaag caggggtgtc cccggaatct caattgttga
     1081 acaaactgga accgcgtttg cagaaagtgg aaaaatattt acaggagcaa acatcagtgt
     1141 tatttaaacg atttccagac aaaatagaac tttacgcctg tggagcggac ttgtcttttg
     1201 ctgctgttaa aaaattagca ccggaagcgt tatcattgct gccctggtgg tggcgtttgg
     1261 aaaaacttcc tgaaagtgac cttgggaatt cgcttcgccg ggaacacttt tttcgatggt
     1321 tgccttatga taaccgaatc gtactggtgc aactgtttgg caggcaaatc gaaattatgc
     1381 tggaatccta tcgacggaaa cagtttgggt ggtatgcgcg cagcagtaca attcttgctc
     1441 cgggaggttt tactgcagaa gtggacaaat ccaggaaagg atcgcggctc tttttaacag
     1501 gtttggatga acatgctttt aacaaggaaa aaatgtatcc ggtatggtta acaaattttc
     1561 attggaacgg tggcggaggt ttaggcgcaa aagctctgtt aaactctgga caattggtac
     1621 gaaaagaatc tgtccatctc agggagctaa tttttcagca cctcagatca ccgtcagtaa
     1681 aactgcctga aaagtgtaag ttgttccttg gaaacagcac aagatctgct tcacataatt
     1741 ctgaagctgg acagtaggat aaaagtatat tattatctac gttttgcatt acaagaaatg
     1801 gctgaagtct tcagtccgct gttttaccaa aagtaggaag atttgcccgt gatggaagat
     1861 gactgatgta gcatccttgc ggtgcagaac gatactttct ttctttacat ttatccctct
     1921 gattgtattt tctgctgaga cgatagttgg ttgtccgtat tacactgtct agtttttcat
     1981 tcatcttgat ctgatgtttg cggttgcagg cgctgactgt ccagtaatct gtgtaacggc
     2041 gacattcgct tgcctccgga agtttttcgt aaacataaac ctcttgactg gaacagcctg
     2101 ccagcacaat tgctccgagg gaaaagacta tacgtgcaaa ttttaacatt ccgaatttat
     2161 ttttatatga tccagttaac atactcgtga gtcattattt cttgtacgat actgatatta
     2221 ttgactgcga catctcatct cgaccgaatg gatagatctg ataaagtgct gatatcttat
     2281 aagatccctc acttacatcc aggatgacac gatagaaaca atgaatgtcg agttaagtta
     2341 tgatagattt tgagaagaac ctccaagctc aaccctgcag agttccattt atattatttt
     2401 ttgttaactt aaaactcctg acaaaattct cccttttaaa tcataatcta agatgagagc
     2461 tgataatcta aatgttattt taaacaacat ggctcgtaat cctgatgatt ggagactcga
     2521 cgaatttcat gcacaccaca aaagcggagt gaaaatttgg atcggtaatg gtgtttttgg
     2581 ctaccatatc gaaaaacccg aatatcagga aattggactg attgagaagt ttaagctgca
     2641 tcggcaatta aaactgctgc ttgaaaataa aaaaactgca tcttaaaatt gctgacttct
     2701 taatcccctg atgaacttgt aatcagtctc aaaaccgtgt catttcaaaa gaaactggaa
     2761 atctttcata agctactata ctaatattat aatgatttat cactgaggtc gaaatgaccc
     2821 agcacctaaa cagcctctgt attattcttt ttttcatttc cagattgctg atttaggtac
     2881 ggggtgtatt tcagactttt tactaatgca tcatatctga cattttttct gtcttatagc
     2941 tgaaaaagtg ttcatttttt tgctgcacac ctgaaaccga tattattgga tgtacttgtt
     3001 ggtaaggcat cacgccgcat ggccgagcgt aaatattcag gaggattact ccaggaacct
     3061 cctcgcagca cccggtactt gcctatttcc ggaccctgcg gatttgaggc gttgtcagtt
     3121 gtatagattc caaaccagtc atagacccat tcccaggcat ttccgtggat gtcaaaaaga
     3181 ccattcggat tacttatttt ttgtgcagtt gtgtgaattg ttctgccgga attttcacga
     3241 aaccaggcat aatcctctaa aaattcagca cccccttcga ctgagtaagc gccctctgtt
     3301 cctgcacgtg cagcaatttc ccactctgct tcagagggca ggcgttttcc aacatagttg
     3361 caataatgcc gtgcagcata ccaggaaacg tgtataaccg gcttcaactc actcccagtt
     3421 tccgggagaa gatcatccgg ccagttcaaa agataattgc cgtcatgaaa tttccttggg
     3481 aaagtgcttt ttcgcagaaa aggatgttgt gcagccacca gtaaaaactg ataatttgtc
     3541 acttcgcatt tgtcgatcca aaattcatcc acctttactt cacgaacagg atgttcagaa
     3601 ggcaggccct ctgaacttcc cattttgaag tttccggaat caatgagtat cataccgggc
     3661 cttgctgttt tttgactgac ttggtcaaca aatccgcaaa gtgtttccaa atccttggtt
     3721 ttgataattg tcgcaacttg ctcttcagga aacaattttt tttccaggtt atatagcagc
     3781 ttaagataag tttttaagat tttcggttca aactttgtaa cccgctttat catattacgt
     3841 tcgtcacgtc cgcagctata tttgtcctta agacatttca tcttttgcct aatccaaatc
     3901 cgtactgact ccctggccct ttgccgctca atttcataca ataaaaaagg ttcagcattt
     3961 aaataaatct tttcaaagag agcagtttga atgagacttg tttttgccag ctccaccaaa
     4021 gcatcataat cctgacaaaa ctttttttga tcatttggaa ccgcaacacc ataggtcctg
     4081 tttagagagc aggctcgtgt ttcgaccttt ttgttgatac caagtttttc ttcaaaaaca
     4141 cgatagcgca actctgaaat ggtgtgcatc agcggataac gctcaccttt aacactgaaa
     4201 ccaataaagt ctgaaatggc agattcctga aactctttag caattttttg aaaattctga
     4261 gctgcagccc agacaaaatt aactaacaaa aaaaatccaa gaaccaaaaa cagaactttg
     4321 taattccacc atttttgttg caattttttc attagtcctt ttttagtata tgactcgtga
     4381 gtcacaaatt ctcctgagat acttatatta ttgactgcgt tttgtcatct agaccgaaga
     4441 gagaaatctg ataaagtgct gttgtcttat aagatccctc acatacgctc gaaatgacac
     4501 gaaaaaactc gtgaatgtcg agtatatgat taaacactaa aaaaactggt agctcacttg
     4561 aaacaaagta gaatgtaggt ttgtctgtca agcttctcta agctagttta agagaaaagc
     4621 aatcgatatt ccatctctaa cagcaaactg gattggtttt cattttgttc ctatatttaa
     4681 ggcaatgcta agttcatgtt ttcattaaat ttcagaaaaa ccggctggct tgcccgttat
     4741 ttaattttta gagcctcaac gccctttacc ggtccagagc cgtatcttga gtttactgga
     4801 gaagattttg gagaagaaaa atttgatgaa ttactctatt tggaagtcga gaaaaatggg
     4861 atgttctttg gatgtccggt tatttcccgg cctgtacaaa acttagcaaa taaattgaat
     4921 tttccaaaac agcaaggtgg aactatttta ctttatctgg aaactctttt ctccattgcc
     4981 ttaattgaaa atgaaagcct gacctcaaat ctacagcatg ctgccacaat tccttatcat
     5041 aatcgcctgc tgaaaattat tttgctggct ttacgctatc atatacccgg catattttat
     5101 cgaattccgg aggacattct tctaacggaa ttgcttgcag aaaatgagac attgcatggt
     5161 gctttgaaac agtttgagga ggaactgtta gattctgtca ccttaaaagg ttattcttca
     5221 cttggcaacc gtcaaaataa ttttgctttt tccaaactct attttttcct gctctggaca
     5281 agggcagaag ccaaaaatga caaatctgaa cccgaagctt ttcttgaaat ggacaaacaa
     5341 ctccgggagg aaatgatttt aacttttgcg gcactgatct gggcagatga ttatgtagac
     5401 agtac
//
LOCUS       DTVD01000222            5394 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5038_length_5394_cov_723.264282, whole genome
            shotgun sequence.
ACCESSION   DTVD01000222 DTVD01000000
VERSION     DTVD01000222.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_5394_cov_723.264282"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(77..1411)
                     /locus_tag="EYO68_07725"
     CDS             complement(77..1411)
                     /locus_tag="EYO68_07725"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIC07731.1"
                     /translation="MKIWLKNPLSILAENAGGGILLEGARIIELIPSGKQPSSEYDSV
                     FDASCHVILPGLINLHHHFYQTLTRVYPQALNKKLFPWLKTLYPLWAGITPEALRMAT
                     RLALAELLLSGCTTASDQHYVFPAGLENAIDLQVEEAQILGIRMVLCRGSMDRSEKDG
                     GLPPDSVVQTCDEILADSERLIQQYHNPEEGAMTQIALAPCSPFSVSETVMRESAKLA
                     EKNNVLLHTHLAETEDENSFCLETIGCRPLEYLEQVGWLNNGTWLAHGIHFTDEEIQK
                     LAATKTGISHCPSSNMVLSSGVCRVMDLEKAGVPVGLGVDGSASNDSSNLMQEVRQAF
                     LLQRLQHGSKVTHLDALRWATEGGANVLRRPELGRIAEGMQADLALFKLDELRFSGNG
                     DPLAALVICGAHQADRVMVAGKWIVEDGQILGLDLEQLQAQHYREAKRLQEK"
     gene            1737..2783
                     /gene="cobT"
                     /locus_tag="EYO68_07730"
     CDS             1737..2783
                     /gene="cobT"
                     /locus_tag="EYO68_07730"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIC07732.1"
                     /translation="MTSFSIPSVSLALNEALQQKIDTKTKPPGSLGRLETLALQVGKI
                     QNTLTPELKSPTILVFAGDHGITEEGVSPFPQEVTAQMVLNFLNGGAAINVYCRQHNL
                     SLHVVDAGVNGDLPEHPDLVAAKIGRGTKNFSKTPAMSAEECEQALNKGAELSRTIPD
                     PDCNVIGFGEMGIGNTSSAAALMQRYTGLPLEDCVGKGTGLDDSGLVRKRKILQKALD
                     QHDKIDEPHEVLATFGGFEIVMMVGAMLESAVLGRILLIDGFIAGSAFLAASRIVPEI
                     QQYAVFTHQSDEQGHSEMLKFLQAEPLLSLGMRLGEGTGAAVAFPLLQSAVAFLNQMA
                     SFESAGVSDRTDGE"
     gene            2773..3564
                     /locus_tag="EYO68_07735"
     CDS             2773..3564
                     /locus_tag="EYO68_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIC07733.1"
                     /translation="MVNSLRQEIRRIVGAFIFYSRIPLPSGWYAKESNHSSRYFSLVG
                     CFVGGASAAVWMLTQMLFSVSSGTSTDGTLPIAVLLGMATAVLLTGALHEDGFADTCD
                     GLGGGWNAEERLRIMKDSRIGTYGALGLVFVVLLKFFALLQIETEILPWVWIAGHAFS
                     RFVSISQLRFASYVQDPAKSKSGSMTEFSGVDLIINAVFGLLPLLFIGNQVWMGLLAV
                     ALVWWLSLVYFKRKLGGVTGDCLGATQQLSEVVFYLCLGLMLVFN"
     gene            3608..4306
                     /locus_tag="EYO68_07740"
     CDS             3608..4306
                     /locus_tag="EYO68_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIC07734.1"
                     /translation="MLKIRKSEERGHVQFTWLDTRHSFSFGSYYDPSFMGFRNLRVIN
                     EDKIAPGRGFPTHGHQDMEIITYMISGALEHKDSMGNYDVIRPREIQHMSAGTGIRHS
                     EYNASDSAEAHVLQIWIEPGKNGIVPEYTQKFVRDVVKPGKLGLLSSPEGKGGVLTMH
                     ADALLYMGILFDNQEIEHPLEVKKHAWVQIVSGELLLNGTKLQAGDGAAVSEESVVKI
                     RSSRSSEFLLFELN"
     gene            complement(4303..5391)
                     /locus_tag="EYO68_07745"
     CDS             complement(4303..5391)
                     /locus_tag="EYO68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIC07735.1"
                     /translation="MFRHAILFSGISVIAWLLKLNGIAWAGYLLWISLIMIATLIIWK
                     AGDFFSPAATYVQEKHDIPQSIKAAVIDAIASSFPEFCVAIIAVILIGRAEVGISTIV
                     GSALYNVLVIPAVAGLVAASPMVISREVVWRDNIYYLGVVLLLGAMLWQFPNEWGAGV
                     AVLFLLAYLGYVFLLQRDFKKAKKQKADSPETEVMKTEVDEDDELELTSEKKAWMWII
                     GMMIVMGGASHLLVESSLALGDMLGIDGVIMGFVVIAAGTSVPDTALSVISARKGQYD
                     AAVSNVFGSNIFDICICLSIPILLALAISGEPTSIDLPQLGLIWSLIGATFLAVYFFW
                     SNNYTLTKLKAGMMGILYLLIILISFTF"
BASE COUNT         1480 a         1142 c         1280 g         1492 t
ORIGIN      
        1 ctacacttcg ccctgaatga caacttttta taaatttgtc agttagttca ttattgggct
       61 ttatagacaa ttgatgtcac ttttcctgca ggcgtttagc ttccctgtag tgttgcgctt
      121 ggagttgctc taaatcaagt cctagaattt gtccgtcttc cacaatccat tttccggcca
      181 ccatcacacg atcagcttgg tgagcaccgc aaatcacgag tgcagccaga ggatcaccgt
      241 ttccggaaaa tcgtaattcg tccaatttaa acagtgcgag gtctgcctgc attccttccg
      301 cgattcgtcc cagttcagga cgccgcaaaa catttgcacc gccttcggta gcccagcgca
      361 acgcatccaa atgagtgact ttagatccat gctgaagtcg ctgaagtaaa aaagcctgcc
      421 ggacttcctg cattaaattt gaagaatcat ttgaagcaga accgtcgacg cccaatccaa
      481 cggggactcc ggctttctcc aaatccataa cccggcagac tccggacgag agcaccatat
      541 tcgaagaggg acaatgcgag attccggttt ttgtggcagc aagtttttga atttcttcat
      601 ccgtaaaatg aatgccgtgt gccagccagg ttccattgtt taaccagccg acctgttcca
      661 gatattccaa gggtcggcag ccgattgttt ccaggcaaaa actgttttca tcttctgttt
      721 cagcaagatg ggtgtgtagc agaacgttat ttttttctgc aagctttgcg ctttcacgca
      781 ttactgtttc tgaaactgaa aagggtgaac agggagcgag tgcgatttga gtcattgccc
      841 cttcctcagg gttatgatac tgctgaatca ggcgttcact gtcagccaat atttcatcac
      901 atgtctgcac gacagaatcc ggaggcaagc ctccgtcttt ttctgagcgg tccatggatc
      961 ctcgacaaag caccatgcgg attcccaaaa tttgtgcttc ttccacttgc aaatctatgg
     1021 cattttccag tcctgcggga aaaacataat gctgatctga ggcggtggta cagccggaaa
     1081 gcagcaattc agccagcgca aggcgtgttg ccattcgcag tgcttccgga gtgattccgg
     1141 cccaaagcgg ataaagtgtt ttgagccatg gaaatagttt tttgttcagt gcctgaggat
     1201 aaacacgggt gagggtttgg taaaaatgat gatgcaggtt gatcaatccc ggaaggatga
     1261 cgtgacaact ggcatcaaaa acagaatcat attcagatga aggctgcttt ccggaaggaa
     1321 tcaattcgat gatgcgtgct ccctcaagca gaataccgcc tccggcattt tctgcaagaa
     1381 tgcttagtgg gttttttagc caaattttca aatctggttt ttgattatat ttttgaacag
     1441 aatttactga attcgaatta aagattttta tattgagagc atagaatttg cgacaatagt
     1501 tttcaagtct ttcaaaataa actgaaacaa agtgctgttt ttttgttttg gagcaatacc
     1561 attttagaat gagattgatt agttgagcaa caaatatata ctggttactt atgtaatgat
     1621 ggtcttgtaa aaagccttta aaacgtgtca tttcgagcga ggcgagaaaa cttttttcga
     1681 ttgcatcatg caataatcac aatccataaa ctcactaaaa aagtaaaaaa ggtaagatga
     1741 catctttttc aataccttca gtttctcttg cattgaatga agcactgcag caaaaaattg
     1801 acactaaaac aaaacctcca ggttcgctgg gcaggttgga aacgctggcc ttacaagttg
     1861 gtaaaattca aaacacgctg actcctgaac tgaaaagtcc gaccatcctg gtttttgccg
     1921 gtgaccacgg aattactgaa gaaggtgtta gtccatttcc ccaggaagtt actgcgcaaa
     1981 tggtgctaaa ttttctcaac ggaggtgccg ccataaatgt atattgcaga caacacaatc
     2041 ttagtttgca tgtggtggat gcaggcgtaa atggagattt accggaacat ccggatttgg
     2101 ttgcagcaaa aatagggcgg ggaacaaaaa acttttccaa aactccggca atgagtgctg
     2161 aagaatgtga gcaggcattg aacaaaggcg ctgagttgag tcgaacgatt ccggatccag
     2221 attgcaacgt aattggtttt ggtgagatgg gaattggcaa tacctcctct gctgctgcgc
     2281 tgatgcagcg ttatacgggc ttgcctcttg aagactgtgt tggaaaaggg acaggactcg
     2341 atgattccgg acttgtacgg aagcggaaaa ttcttcagaa agcattggat cagcacgaca
     2401 aaatagatga accgcatgaa gtgttagcga cttttggagg ttttgaaatt gtgatgatgg
     2461 ttggggcaat gcttgaatca gccgtattgg gacggatttt actgattgac ggttttatcg
     2521 ccggctctgc ttttcttgct gcttccagaa ttgtcccgga aattcagcaa tatgccgttt
     2581 ttactcatca gtctgatgaa cagggacata gtgaaatgtt aaaatttttg caggcggaac
     2641 ctttgctttc attaggaatg cgtcttggtg aaggaactgg ggccgcagtc gcttttccgc
     2701 ttttgcagtc agcagtcgct tttctaaatc aaatggcatc ctttgaatct gcaggtgtta
     2761 gtgaccgaac tgatggtgaa tagcttaaga caggaaattc ggcgcatagt tggtgccttt
     2821 atcttttaca gcaggattcc gctcccttca ggttggtatg ctaaagagag taaccattcc
     2881 tcgcgctatt tttcacttgt gggctgtttt gttgggggag caagtgcagc agtttggatg
     2941 ctgactcaaa tgctgttttc agtatcgtcg ggcacatcaa cggatggcac tttgccgatt
     3001 gcagttttgc tgggaatggc aaccgcggtt ttgctgaccg gtgccttaca cgaagatgga
     3061 tttgccgata cgtgcgacgg actcggtggc ggatggaacg cagaagagcg gctgcggata
     3121 atgaaggatt cccggattgg aacttatgga gcacttggtt tagtattcgt ggtgctgctg
     3181 aaattttttg cgcttttaca aattgaaacc gaaattttgc cctgggtttg gattgcaggc
     3241 catgcattta gccgttttgt ctcaatttca cagctccgtt ttgcaagcta tgtgcaggat
     3301 ccggcaaaga gcaaatccgg atctatgact gagttttcag gggttgattt aattataaat
     3361 gctgttttcg gcttgttgcc attattgttt atcggtaacc aggtctggat gggattgctg
     3421 gcagtcgcac tggtctggtg gctatcatta gtttatttta agcgcaaact tggaggagtg
     3481 acaggtgact gcctgggcgc aactcagcag ttaagtgaag tggtttttta tttgtgcctg
     3541 ggattaatgt tggtgtttaa ttaaaatttc gatttctgcc caaatcattt agtgaacaaa
     3601 gtggattatg cttaagattc gcaaatcaga agagcgtggt catgtacaat tcacctggct
     3661 ggacacacgg cacagctttt cgtttggtag ttactacgac ccgagtttca tgggtttccg
     3721 aaatttacga gtgatcaatg aagacaagat cgctcccggc cgtgggttcc caactcacgg
     3781 tcatcaagac atggaaatta tcacttacat gatttccggt gcgctggagc ataaagacag
     3841 tatgggtaat tacgacgtga tacgtccgcg tgaaatccag cacatgagcg cgggaaccgg
     3901 aattcgccac agcgagtata acgcttcaga ttcagcagaa gcacatgtac ttcagatttg
     3961 gattgaaccg ggaaaaaacg gaattgtgcc ggagtacaca caaaaatttg tgcgtgatgt
     4021 tgttaaaccc ggaaagctgg gtttgctgtc gtcacctgaa ggaaaaggag gggttctcac
     4081 aatgcatgcc gacgcattgc tctacatggg gattttattt gacaaccagg agattgagca
     4141 tccgttggag gtaaagaaac atgcgtgggt gcagattgtg agtggagaac ttttactgaa
     4201 cggaactaag ttgcaagctg gagacggtgc ggctgtcagt gaggaaagcg ttgtgaagat
     4261 tcgttcctcc agatcgagcg agtttctgct ttttgaacta aactaaaacg taaaggaaat
     4321 taaaataatt agcaggtaga ggatacccat cataccggct tttaatttgg taagagtata
     4381 attattgctc caaaagaaat agacggccaa aaatgtggca cctatcaggc tccagatcag
     4441 tcccaattgc ggaagatcaa tgctggttgg ttctccacta atggcgagtg ccagcaaaat
     4501 tggaatgctt aaacatatgc agatgtcgaa aatgttgctg ccgaatacat tggaaaccgc
     4561 tgcgtcgtac tgtcctttcc tcgcacttat taccgagaga gctgtatccg gtactgatgt
     4621 tcctgctgca atcacaacaa atcccatgat aactccgtca attcccaaca tgtcaccaag
     4681 tgccagggaa gattcaacaa gaaggtgaga tgctcctccc atcacaatca tcatgccgat
     4741 gatccacatc catgcctttt tttcggatgt tagttccagc tcatcatctt cgtcaacttc
     4801 agttttcatt acttcggttt ccggagaatc tgctttttgc ttttttgctt ttttgaagtc
     4861 cctttgaaga agaaatacat aacctagata ggccagcaaa aaaagaacag ctacacccgc
     4921 accccattca ttgggaaact gccagagcat tgcaccgagc aaaagaacca caccaagata
     4981 ataaatatta tcacgccaga cgacttcgcg actgattacc attggacttg cggcgaccag
     5041 tcctgccaca gcaggaataa ccaaaacgtt ataaagcgca gatcccacga tagttgaaat
     5101 gccaacctcc gcccgtccaa tcaagattac agcaataatg gctacacaaa actccggaaa
     5161 ggaactcgcg attgcgtcaa tgaccgcggc tttaattgat tgtgggatat cgtgtttttc
     5221 ctggacataa gttgctgccg gagagaaaaa atcacctgcc ttccaaatga ttaaagtggc
     5281 aatcataatt agtgaaatcc acaacaaata accagcccat gctataccgt taagtttcaa
     5341 gagccaggca atgactgaaa ttccggaaaa gagaatggcg tgccggaaca tatc
//
LOCUS       DTVD01000223           18834 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_515_length_18834_cov_451.643698, whole genome
            shotgun sequence.
ACCESSION   DTVD01000223 DTVD01000000
VERSION     DTVD01000223.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 18834)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18834)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18834
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_515_length_18834_cov_451.643698"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="EYO68_07750"
     tRNA            complement(1..77)
                     /locus_tag="EYO68_07750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(41..43),aa:Pro,seq:ggg)
     gene            complement(171..626)
                     /locus_tag="EYO68_07755"
     CDS             complement(171..626)
                     /locus_tag="EYO68_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIC07736.1"
                     /translation="MPTEKLLQLLKEITEEKIPFNKLIGMKIETLDLDKIGIRFEMRP
                     ELVGNFTRGNLHGGVISSVLDVTGGMVAWTGIMKKMEGQSFDEISERFNKIGTIDIRV
                     DYLRPGLGEYFIATGSTLRTGNKVSVTRMELHNDKGILIAVGTGTYVVG"
     gene            complement(720..1481)
                     /locus_tag="EYO68_07760"
     CDS             complement(720..1481)
                     /locus_tag="EYO68_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIC07737.1"
                     /translation="MNQALSLENPGTSGVFFDKRLGTNLVAAALVGGGLWFNGPWRET
                     LLNTGLFALSGAITNWLAIYMLFERVPGLYGSGVIPLHFEDFKTGINELIMHQFFNRE
                     NVEKFFANSSGLKIFPDFEAVFKKVNLNPAFDSLLKVIENSSFGPMLSMVGGVQVLEP
                     LREPFKEKLQVAVQKISKTEPFKEALREQLADLSASDDILQKVEGIVSKRLDELTPNM
                     VKEIVERMIQEHLGWLVVWGGVFGGLIGLITTFVF"
     gene            complement(1556..2080)
                     /locus_tag="EYO68_07765"
     CDS             complement(1556..2080)
                     /locus_tag="EYO68_07765"
                     /inference="COORDINATES: protein motif:HMM:PF11220.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HIC07738.1"
                     /translation="MKKEIVLQRVFAVLLLLVSSVQFVQARHKCYKVPAPPGIMGIFS
                     SITLIPSGSTMAAARSSETSGCDRGHPSENFYKPKNKRVALFLKDNFLQVREESAQGQ
                     GLHLDALAQLAGCSIEHAEFGRIIQNNYSQIFGADLLSQNADNSENITKRTTERFLGL
                     MADSPLLASSCESG"
     gene            complement(2141..2863)
                     /locus_tag="EYO68_07770"
     CDS             complement(2141..2863)
                     /locus_tag="EYO68_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006645404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIC07739.1"
                     /translation="MISIDSMHKWFDDFHVLKNINLEVKRGEKVVICGPSGSGKSTLI
                     RCINRLEEHQQGSIVVDGTELTNDLKNIEIVRSEVGMVFQHFNLFPHLTALENCTLAL
                     IWVRKTSKAEAEKTAMEFLERVKIPEQANKFPGQLSGGQQQRVAIARSLCMNPRIMLF
                     DEPTSALDPEMIKEVLDVMIELANSGITMLVVTHEMGFATAVGDCVIFMDEGEIIEKN
                     NPKDFFNNPQHDRTKLFLSQIL"
     gene            complement(2936..3937)
                     /locus_tag="EYO68_07775"
     CDS             complement(2936..3937)
                     /locus_tag="EYO68_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIC07740.1"
                     /translation="MSFLLLWSIIPPFFEWSVTNSIFTADSRIQCWDQMAVPGAGACW
                     AFIDDRLALFTYGFYPEPLRWRVNLSFVLLILAIIPVLYEKMPGRKYGLWYSAIFPFI
                     TGWLLVGGEVNGFGFEPVDTDQFGGILLTLILGVTGISFSLPIGIALALGRQSKLPVL
                     RIMCVLFIEFIRGVPLITLLFVASTMLAYFLPPGTNFDLLLRVLIMVTLFASAYMAEV
                     IRGGLQAISRGQHEAGDSLGLTYWQANRLIVLPQALKISIPGIVNTFIGLYKDTTLVL
                     IIGMMDILGLGGASLADVKWAGLANEIYLFVALYFFVTCFSMSRYSLYLEKKLHTGH"
     gene            complement(4085..5281)
                     /locus_tag="EYO68_07780"
     CDS             complement(4085..5281)
                     /locus_tag="EYO68_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC07741.1"
                     /translation="MSTPASTENHPNILLRLWRDKDTRSIFIQIITMVIVFTFLGLII
                     HNVVINLEIAGKDFSFGFLNYPAGYDITFQPFISYSPTDTHLRAAAVGILNTLLVAVS
                     GVIIASILGFTMGVLRLSNNWLISRLVYVFLEFTRNVPVLLHILFIYSIFLYVLPVPK
                     KAINISDTVFLTNRGFILPSPVFEDGFGFVWIALLAAIAIVVFFVRWERKNQDATGEH
                     YPVFSISLAILFGLPLIVFFISGAPMAWEIPALKGFNFKGGTSIKPEYIALTFALSYY
                     TACFIAEIVRAGILAISRGQTEAALALGLLPNRSLQLVVIPQALRVIIPPLASQYLNL
                     TKNSSLAIAIGYMDVVATVGGITLMQTGKEMETMIIVLLTYLFFSLIISAFMNWFNRR
                     MALVER"
     gene            complement(5396..6412)
                     /locus_tag="EYO68_07785"
     CDS             complement(5396..6412)
                     /locus_tag="EYO68_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07742.1"
                     /translation="MRALAKIALTLTAVGAMAFTATAGTLEDVKARGVLRCVVSTGIA
                     GFAYPDADGTWKGFDIDFCRATAAAVLGDSSKIKAITSTGKTRFTKLNSGEGDVLWRN
                     TTITFSRDVGVKLTFVGVNYYDGQGFMVNKSLGVNSAKELDGASVCIQTGTTTELNLA
                     DFFRANNMSYEAVTIEKNSEARAAYLSGRCDIYTTDASGLAATRSTFPNPGNHKILPE
                     IISKEPLGPAVRQGDDQWADIVRWIYNATVTAEELGVTSSNVDSMKGSNNPEILRLLG
                     VEGSQGEELGLSKDWAYQVIKQMGNYGEIFERNIGTNTPIGLARGLNDQWNKGGLQYS
                     PPFR"
     gene            complement(6578..8881)
                     /gene="menE"
                     /locus_tag="EYO68_07790"
     CDS             complement(6578..8881)
                     /gene="menE"
                     /locus_tag="EYO68_07790"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HIC07743.1"
                     /translation="MFDVNPNILNQVLEHYENQPFLISEKRSWTFADFMSEARLLSDS
                     LMAESQQRVYLSSENPENLLKSMLALWMHGAVAVPLNPQIPEKQKMEMLQKIDCTFSY
                     TELQHEFKSHPTSENSLQPNPVGNSNAVAGKEVNSINPKDWATIIFTSGSTGSPKAVV
                     HSLANHFYNALGANERMPLNPGDRWLLSLPMYHVSGLAILFRTLLSGAAMVIPAKNVS
                     LSKTLLSRSVTHLSLVPTQLHRLLQTTKGKDALCQLKLILLGGASIPETLIEQSAQLG
                     LNVQTTYGSTEMASQVATGKSGFYQVLPFREVRIAADNEVEVRGKIGFLGYLDGTGLH
                     QPFDENGWFKTGDIGLWCSKDSKNQGDVVSLKITGRKDSMFISGGENIHPEEIERILL
                     QFGKTEQAVVVAVQDAEFGARPVAFLKTREDFSESKLQNFLEKRLAKFKVPDLFLALP
                     ELVESEIKHNRVELSKFAQTQFDLWRKGNTKNNGKTAIFNQWLNQFQLGWMRVALSEE
                     RQIFSLLDLRDKGNMRCLHLRANSRKEVMEWLLAAENRKLLEVLQNGNQMTKTGLKWI
                     PVTATKRGAVKESFEIVRLLADELPENKTPLYDAQDRGTFKTLLMPKKILIKKSTENL
                     QSNHSGHLSAAASKTFHSEPECLNSQAPVFKKKWPWEFHEAVFQTGVFLPKLNSMFLL
                     RCLYRRIASGLQFLGWKVQLMRGLSCKDNMEHPFWELSLEAEKLIENELCKSGLCSTN
                     DFRQANTPKIERKRRADFQTKFDNSFS"
     gene            complement(8881..10038)
                     /gene="menC"
                     /locus_tag="EYO68_07795"
     CDS             complement(8881..10038)
                     /gene="menC"
                     /locus_tag="EYO68_07795"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIC07744.1"
                     /translation="MVDWIKMSLCRWKLFHYSLPLTEPLYLLGHEMHGRTGLILRLHD
                     ENDGTSVGDNYGEGEIAPLPGMHPETLSEAENQIRDYLSGNSLPVTCSAALFGSVNFG
                     LDMALRTMFQSPNVSKFHSFKETAGNDPKPSKIDFTGQIFPVNGLAVGSGTVLEMECE
                     ELRNGGFKAVKLKVGQLTILQDIERVRLARKILGDEIALRLDANQAWEWEDAVKFAEA
                     VQDFNIEYCEEPLLDSNKLEKLHLQTGMPLALDETLWYAPTPKSDTPAKHVSLSGIRA
                     LILKPGILGGWNNTKMWIEHAQKNGIHCVLSSCFESGLGLNWIAFMANELLSEQFPAG
                     LDTSKWFEQDLIEPRFLIQNGNYVFPDSWPKSKDTFLEIISEGTCEIGDLD"
     gene            complement(10023..10913)
                     /locus_tag="EYO68_07800"
     CDS             complement(10023..10913)
                     /locus_tag="EYO68_07800"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIC07745.1"
                     /translation="MNISNYLRVWLSAIRPKTLGAAVSPILIGTAMAFGEGKGHALAA
                     LAALLGALLIQIGTNFSNDYFDYIKGADTEERLGPVRVTQSGQVRPKTMLWNFVMVFG
                     LATLVGIYLVFRGGWPIVIIGILSIASGILYTGGPWPLGYLGLGDLFVLIFFGPVAVG
                     GTFYVQTLEITPVVIIAGLGPGLLATALLAVNNLRDEPTDKKVGKLTLAVRFGTTFAR
                     AEYLAALVFSAVIPFILAFWTETHWYACISALIIIPGFSSIRQIISGVKGSELNITLA
                     STGKLILIYGILFSLGWWIG"
     gene            complement(10971..11798)
                     /gene="menB"
                     /locus_tag="EYO68_07805"
     CDS             complement(10971..11798)
                     /gene="menB"
                     /locus_tag="EYO68_07805"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIC07746.1"
                     /translation="MNNNYWKSCGSYTDINFEKSNEGIAKITINRPEVRNAFRPLTVR
                     EMRAALNDAREDTKIGVIILTGEGEKAFCSGGDQRIRGSAGYEDSETGHLRLNVLDFQ
                     REIRTCPKPVVAMVAGYAIGGGHVLHVMCDLTIAADNAIFGQTGPKVGSFDGGYGSSY
                     LARMVGQKKAREIWFLCRQYTAQQALEMGMVNTVVPLEKLEEETLQWSREMLQHSPMA
                     LRCLKSALNADCDGQAGLQELAGNTTMLFYMSEEGQEGRNAYLEKRKPDFNKFTRYP"
     gene            11994..12491
                     /gene="rplU"
                     /locus_tag="EYO68_07810"
     CDS             11994..12491
                     /gene="rplU"
                     /locus_tag="EYO68_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIC07747.1"
                     /translation="MFAIIRTDGKQYRVAQGETIRLARISAEPGDAFETDQVLAIGGE
                     ESELKFGKPVIVGAKVQGTILQHGKDQKVIVFKRKRRKTYRRKYGHRQAHTLVKIDKI
                     SAKAPAAKKSVPEKITAEKDTANKTPAKKAAPVKVAAKKAAPVKAAAKKAAPKKTTAK
                     KAADK"
     gene            12513..12800
                     /locus_tag="EYO68_07815"
     CDS             12513..12800
                     /locus_tag="EYO68_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIC07748.1"
                     /translation="MAHKKAFGSSRNGRDSQGQRRGVKKFGGELVKAGNILVRQVGST
                     FHAGLNVGTGRDFTLFSKVSGHVQFIQKGSGKHKRKYISVIADDAAVSASV"
     gene            12804..13775
                     /gene="trpS"
                     /locus_tag="EYO68_07820"
     CDS             12804..13775
                     /gene="trpS"
                     /locus_tag="EYO68_07820"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008047722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIC07749.1"
                     /translation="MGAKPRSLSGIKPTGTPHLGNYLGMIKPAIQLQKTHETFYFIAD
                     YHALTSTRDPEKLREYTNDLTAIFAALGMDFENHAFFRQSDIPEVTELTWILSCITSK
                     GMMERAHAYKDAVSKEQDVSMGLFCYPVLMACDILIYDSNFVPVGQDQRQHLEITRDL
                     AIRFNHLYGDALVVPEAIIADDVATIPGLDGRKMSKSYGNIIPLFASEKQFRKAIMKI
                     TTDSKSVDEAKDPEACSVFALFKCFADKADQEALAERYRAGDMGYGEAKQLCFEKLNA
                     ELHEPREIYQQIRNDQAKLDGILESGRDKARVIARQVTDRVRNKVGL"
     gene            13901..15325
                     /locus_tag="EYO68_07825"
     CDS             13901..15325
                     /locus_tag="EYO68_07825"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIC07750.1"
                     /translation="MEMIPDILASRYASPEITSIWSPEGKVRLEREFWIAVMKAQRKL
                     GVDIPETAIAAYENVRDKIDLESISQREQKLRHDVKSRIEEFCELAGYEHIHKGLTSR
                     DLTDNVEQLQILRSLRLVRSKAVAVLHQLADWSLRTKDIMLVGRTHNVPAQPITLGKR
                     LAMFGEEIMFGLGRLDHLIENYPLRGLQGAVGTRLDQLVLMNGDKKKVQELGEQVLEH
                     LDCSTSFNAVGQVYPRSLDVETVRALIALSSGISSFAKTLRLMAGQGLASEGFQQGQV
                     GSSAMPHKMNARTSERINGFHQILNGYGTMLSGLSGDQWNEGDVSCSVVRRVALPGAF
                     FASDGQLEAALTVLSEMGFFEAAIAQELQQYLPFLASTTLLMEAVRKGGGRESVHEAI
                     KENAVAVADALRNGDLGENDLSQRLADDERVPLDLQEIEAVLNDPERFAASAPEQAEA
                     FANQVRKWTERFPEAKKLVPQVLL"
     gene            complement(15379..16332)
                     /locus_tag="EYO68_07830"
     CDS             complement(15379..16332)
                     /locus_tag="EYO68_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07751.1"
                     /translation="MRFIKLITLFLFLSAFALQPVLAKKIPLGMLVDIKGKIEYSKKG
                     KRWKKVRRNKFVYANYLVRVSADSSIKFLNQETNETTLLTANSKIKFTKKGLEVLEGS
                     LGGTDAGGGLLSGLSKQFKKTQKYTTVRRAAKKGGINLKLATNTISADFSELAWETAG
                     ADYAYRLHLGTKDRKTNTWTDSAVYDVPATGDEVVRTRIKPFSKKQKYFVEVLDGSGV
                     VAYTAKAANLKVLSGKKLAKFKKQKDEFQSMDESGFLYAGLLKDNGLLVPALDQYEQF
                     FTEFSDDEDINELRPFLIEVYSRLRLEHIKSVELKKYESAE"
     gene            complement(16647..17456)
                     /locus_tag="EYO68_07835"
     CDS             complement(16647..17456)
                     /locus_tag="EYO68_07835"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03473.15,HMM:PF03476.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIC07752.1"
                     /translation="MTNPVLSQLNIYPLKSASGISLDNAFMEQRGLAHDRRWMVVDDS
                     GQFMTQRTCPSMALINTELVGQTLTLNAPRMSELSLPLFPTKGESQEVEIWGDRCEAW
                     TTNPQSKLWISEYLGKSCNIVFMPDHSNRPVDPDYASAQNQVSFSDGFPLLLISESSL
                     SDLNNRLPESLAMKRFRPNMVVKNTEPYAEDNWKKIRIGECEFQIVKSCSRCILTTVD
                     PETGKFSGKEPLRTLAGYRKQNGNVQFGQNLIATINGKLSVGMPVEIVTQS"
     gene            complement(17491..18177)
                     /locus_tag="EYO68_07840"
     CDS             complement(17491..18177)
                     /locus_tag="EYO68_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07753.1"
                     /translation="MLNQTSVTEITSEEILQQMIRETFEVPKIEKLASEENFPGAGFS
                     QQIERDSESKENLLVQRLVNTLTAQLQTILPGAFRITSETEQCLSYANLSSWSPGRSY
                     FSLFEMKHSNSVWILHFSRTVGEGLASLVHTKNSRNHANIFNDLREADSITYLEIGEM
                     LRKFFVSLLELWPKSDKLKVASCRHILQLGFLRDSVMDEEYIVLPFYLDNRECSGKFH
                     LVFPHRYLLL"
     gene            18346..>18834
                     /locus_tag="EYO68_07845"
     CDS             18346..>18834
                     /locus_tag="EYO68_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07754.1"
                     /translation="MFKDRLRINLTERKKNRDITLSDSKFMQKSLGIIIIFLFCFSGG
                     LFFEQNLGAESQGRKIQSYVKNGTVLQARKREARILEQKNKGRKVKYYVLKKSVLQGR
                     KSIAKMSANKMLETGLVTSKMVKNNQWFVKKPFQPRYTKVNIVSKYTPPEVNPEESDK
                     ISR"
BASE COUNT         5508 a         4076 c         3943 g         5307 t
ORIGIN      
        1 tggtcggagc gactggattt gaaccagcga ccgcacgccc cccaggcgtg tgcgctacca
       61 ggctgcgcta cgctccgatt gtgtatctat gatgaagagt ttttcagcta agtgtcaagt
      121 cctttcacat tcatccgatg tcggctgttt atgcttttgc gggaattaac tcagccaact
      181 acataggtac cggtaccgac tgcaatcaga attcctttgt cattgtgtaa ttccatacga
      241 gtaacagata ctttgttacc tgtacgcagg gtactcccgg ttgcaataaa atattcgcct
      301 aaacctggac gcaggtaatc aacacgaata tcaatagtac ctattttatt aaaacgttca
      361 gaaatttcat caaatgactg gccctccatt tttttcatta tcccggtcca ggcgaccata
      421 cctcctgtta catccagtac agaagagatt actcctccat gcaggttgcc ccgagtgaaa
      481 tttccgacca gttcaggacg catttcgaag cggatcccga tcttgtctaa atccagcgtc
      541 tctattttca ttccaattaa tttattgaac ggaatttttt cttcagtaat ttcctttagt
      601 aactgcagga gtttttctgt gggcatgata ggattcagga ttcaggattc aggattatag
      661 attagaggga ttaacaccgg ttgtttactg aaataattct ctatagttag tgaacgtttc
      721 taaaatacaa aggtcgtgat aaggccaatc agaccgccaa agacacctcc ccagacaacc
      781 agccagccaa ggtgttcttg aatcatccgc tcgacaatct cttttaccat gtttggtgtg
      841 agctcatcca ggcgtttaga aacaatgcct tcaacttttt gcagaatgtc atcacttgca
      901 gaaaggtcag ccagttgttc acgtaatgct tctttaaaag gttcggtttt cgaaattttc
      961 tgtactgcaa cctgcagctt ttccttaaaa ggttcgcgca gaggttccag aacctgtact
     1021 cctcccacca tggataacat tggaccaaaa ctggagtttt ctatcacttt cagcaacgag
     1081 tcaaaagcag gatttaaatt tacttttttg aagacagctt caaaatcggg aaaaatcttt
     1141 aaaccggaag aatttgcaaa gaacttttcc acattttcac ggttaaaaaa ctgatgcata
     1201 attaattcat tgatgccggt tttgaaatct tcaaaatgca gcggaatgac accggaacca
     1261 tacaaacccg gtaccctctc aaaaagcatg taaatagcca gccagttggt aattgcccct
     1321 gaaagagcaa aaagtcctgt gtttaacaga gtttctcgcc atgggccatt aaaccataac
     1381 ccgccaccaa ccaaagccgc cgcaaccaga tttgttccta aacgtttgtc aaaaaatact
     1441 ccggaagttc cgggattttc aagtgaaaga gcctgattca tgaacttttg cttagcaaaa
     1501 aattaactga atgttggaat ttgagttttg gtaaaaatca gtttgtcgga ggaatctaac
     1561 cggattcaca actggaagct aataacggtg agtctgccat caaacctaaa aaccgttcgg
     1621 tggttcgttt tgttatgttt tcagaattat cggcattttg tgataataag tctgcgccaa
     1681 aaatttggga ataattattt tgaattatcc tgccaaattc agcatgttcg atggaacatc
     1741 cagccaactg agcaagtgca tccagatgaa gtccttgtcc ttgagcactc tcctcacgga
     1801 cttgcaaaaa attatctttg aggaacaaag caacacgttt gttctttggc ttatagaaat
     1861 tttctgatgg atgtccacgg tcacaacctg atgtttcgga ggagcgtgcc gcagccatcg
     1921 tgcttccgga aggaatgaga gtaatgctgc tgaaaatgcc cattatacca ggtggtgccg
     1981 ggactttgta acacttgtga cgagcctgca caaattgaac actacttacc agcaaaagca
     2041 atactgcaaa cactctctgg agcacgattt cttttttcaa ctaaaatctc ctgaaagttc
     2101 cctgagcggg tttcccaaaa agttgattgt ctaatttctg ttaaagtatc tggctcaaaa
     2161 aaagctttgt cctgtcgtgt tgtgggttat taaagaaatc ctttgggttg tttttttcaa
     2221 taatttctcc ctcgtccata aaaataacgc aatccccaac tgctgttgca aatcccattt
     2281 cgtgggtcac aaccagcatt gttattccac tgtttgcaag ttcaatcatt acgtccagaa
     2341 cttccttgat catttcagga tcaagagctg aagtcggttc gtcaaaaagc attattctgg
     2401 gattcatgca caaggagcgg gcaattgcaa cacgctgttg ctgaccacct gaaagctgac
     2461 cgggaaattt attagcctgt tcagggattt tcactctttc cagaaattcc atggctgttt
     2521 tttccgcttc ggcctttgag gtttttctga cccaaatcag agcaagtgta cagttttcaa
     2581 gagcagtcag gtgtggaaac aaattaaaat gctgaaatac catacctact tctgagcgta
     2641 cgatttcgat atttttgaga tcatttgtca gttctgtacc gtcaaccaca atacttccct
     2701 gctggtgttc ttcaagtctg ttaatgcaac ggatgagagt agattttccg gaaccggatg
     2761 gtccgcagat gacaaccttt tcaccacgtt taacttccag gttaatgttc ttgagaacat
     2821 gaaagtcatc gaaccacttg tgcattgaat caatggaaat catgatttcc gtttcacttg
     2881 ttttagtatc ggctattgta gacatgagaa atttttgggt tttaattttt aacttttaat
     2941 ggcctgtatg cagtttcttt tccaggtaaa gtgagtaacg ggacatgctg aaacaagtga
     3001 cgaaaaaata cagagctaca aaaagataaa tctcgtttgc taaccctgcc cactttacat
     3061 ccgccaatga agcccccccc agacccaaga tatccatcat accgatgatc agtaccaaag
     3121 ttgtatcttt gtaaagtcca atgaaagtat ttactatacc cgggatcgag atttttagtg
     3181 cctgcggcag tacgatcaac cgattcgcct gccagtatgt cagtccaagt gaatcaccgg
     3241 cctcatgctg acccctggaa attgcctgta aaccgccgcg gatgacctcg gccatgtatg
     3301 ccgaggcaaa gagggtcacc attatcagaa ctcgcaaaag taaatcaaag ttggttccgg
     3361 gaggtagaaa gtaggccagc attgtagaag ccacaaagag caaagtaatc agaggtacac
     3421 cacgaatgaa ctcgatgaaa agtacacaca taattctcag caccggcaat tttgattgac
     3481 gtccaagagc caatgcaata ccaatcggca gcgaaaagga aattccggtt acaccaagaa
     3541 tcagtgtaag caggataccg ccaaactgat cagtgtctac aggttcaaag ccgaatccgt
     3601 tgacttcacc tccgaccagc aaccatccgg taatgaatgg gaaaattgct gaataccata
     3661 agccgtattt tctgccgggc attttttcat aaagtacagg aatgatagcg aggatcagca
     3721 gcacaaatga caaatttaca cgccaacgca aaggctcggg ataaaaacca taagtaaaaa
     3781 gagctaaacg gtcatctata aaagcccagc aggcgcctgc tcccggcact gccatctgat
     3841 cccagcattg tattctggag tctgcagtga agatggaatt cgtaacactc cattcaaaaa
     3901 atggaggtat tatcgaccat aacaacaaga aagacattat agtcagtaca gtgttcaggg
     3961 gcgatgaaaa caaatttttt ctggcccagg ccagcggtcc gctggtactg acaggtggcg
     4021 gccaatccgg atgagttccc gggaggtaat tttttggagt ggtattattc actttcaatc
     4081 tgcgttatcg ttcaaccagc gccattctgc ggttgaacca gttcataaat gctgaaataa
     4141 ttagtgaaaa aaacaaataa gtcagaagta caattatcat tgtctccatc tctttacccg
     4201 tttgcatcaa ggtgatgcct ccaacagttg ctactacatc catgtaacca atggcaatag
     4261 ccaaagaaga gtttttggta agattgagat attggctggc caggggtggt attatcacac
     4321 gcagtgcctg cggtatgacg accagctgca gggaacggtt tggtagcaaa ccaagcgcga
     4381 gtgcagcctc ggtttgtcca cgactgatag ccaaaattcc tgcacgtacg atttccgcaa
     4441 tgaaacaggc agtgtaatat gacagggcaa atgtgagtgc tatatattct ggttttattg
     4501 atgtaccacc cttaaagttg aagcctttca atgcaggaat ttcccatgcc atcggagcac
     4561 ctgaaataaa aaatacaatc agcggtaagc cgaacaggat ggccagggaa atgctgaaca
     4621 cgggatagtg ctcaccggtt gcatcctgat tcttgcgttc ccagcgtaca aaaaatacca
     4681 caattgctat tgcagcaagc agtgcaatcc agacaaatcc aaaaccatcc tcaaaaaccg
     4741 gtgacggtaa aataaatcca cgattagtta aaaatactgt atctgagatg ttgattgctt
     4801 tttttggaac aggaagaacg tagaggaaaa tgctgtagat gaaaagtatg tgtaagagaa
     4861 ccgggacatt cctggtgaat tcaagaaaaa catagacaag cctgctgata agccaattgt
     4921 ttgaaagtct caaaactccc atggtgaagc ccaatattga agcaattata actcctgaaa
     4981 ctgcaaccag gagggtattt aaaattccaa ctgctgctgc tcgtaaatgg gtgtcggtgg
     5041 gtgaataaga aatgaatggc tgaaaggtaa tatcgtagcc tgcaggataa tttaaaaaac
     5101 caaagctgaa gtcttttcct gctatttcca gattgataac aacattatgt attattaacc
     5161 ccaaaaaagt gaacacaatc accatcgtga tgatttgtat aaaaatagaa cgggtgtctt
     5221 tgtcacgcca caaacgcaac aaaatattag ggtgattttc agtagaggca ggtgtgctca
     5281 ttgtgtgaaa tttcccgttg aactgcaggg atcaattgct tgtgtggaac gtaaaagcgc
     5341 acatactcaa agggagaata tgtgcgctca aatcaacgtt taattaaaac gaagattaac
     5401 ggaatggtgg agaatactga agtcctccct tattccattg gtcattcaaa ccacgcgcca
     5461 agccaatcgg tgtatttgta ccgatattgc gctcgaagat ttcgccgtag tttcccattt
     5521 gctttatgac ctgataagcc cagtctttgc tcaagccaag ctcttcgcct tgactgcctt
     5581 caacaccgag aagacgcaga atttcagggt tattggaacc cttcatgctg tccacgtttg
     5641 aggatgtaac acctaattct tcagctgtta cagtcgcatt ataaatccag cggacaatgt
     5701 cggcccattg atcgtcacct tgacgaactg cgggacccaa cggctccttg gaaatgattt
     5761 ccggaaggat cttatgattg cccggattag ggaaggtaga gcgggttgcg gccaaacctg
     5821 aggcatcagt tgtgtaaatg tcacaacgtc ctgacagata agctgcacgt gcttccgagt
     5881 ttttctcgat agtcaccgct tcatatgaca tgttgtttgc acggaagaaa tcggcaagat
     5941 tcaactcagt ggtggttccg gtttggatac agaccgaagc accgtccaac tctttcgcac
     6001 tgttgacacc aaggctctta ttgaccatga aaccttgtcc gtcataatag ttgactccaa
     6061 caaaagtcaa ctttacacca acatcacggg aaaaggtgat agtagtgtta cgccataaaa
     6121 cgtcaccttc gccggaattc agcttggtga aacgtgtttt acctgttgag gtaattgcct
     6181 tgattttgga cgagtcgccc aaaactgcgg cggcggttgc ccggcagaaa tcaatgtcga
     6241 aacccttcca ggttccgtct gcatccggat atgcaaatcc ggcaattcca gtactaacca
     6301 cacagcgaag cacaccacgt gctttcacat cttcaagtgt tccggctgtt gccgtgaacg
     6361 ccatggcacc gactgcagtc aatgtcagtg caatttttgc aagtgctctc atatcaatct
     6421 ccttttgaac acattataaa aaaccatccc atacatttgg aacagacctt atcaacgccg
     6481 cctattgtat tgatttttca ggatctgtca agtctaaaat acaaaaaaat atttagtttt
     6541 attatattct tgatgtcagt aaaaaatttt tgctgattta actgaacgag ttatcaaatt
     6601 ttgtttggaa atcagcacgg cgtttacgtt caattttggg tgtatttgcc tgtcgaaaat
     6661 catttgtgga acacaaaccg gacttgcaca attcgttttc gattaatttt tctgcttcca
     6721 agctgagttc ccaaaatgga tgttccatat tgtctttaca tgacaatcct ctcatcaact
     6781 gaactttcca accgaggaac tgcaaacctg aagcgatgcg tctgtaaagg caacgcagca
     6841 aaaacatgct gtttaatttt ggcaggaata ctccggtttg gaaaactgct tcatgaaatt
     6901 cccagggcca ttttttttta aaaacaggtg cctgggaatt aagacattca ggttctgagt
     6961 gaaaagtttt tgaggcagca gcggacagat gacccgagtg gtttgactgc agattttctg
     7021 tcgatttttt tatcagtatt ttcttcggca ttaataatgt tttgaatgtc ccacgatcct
     7081 gcgcatcgta cagtggcgtt ttgttttccg ggagttcatc ggctaaaagc cggacgattt
     7141 caaagctttc tttgaccgcc ccgcgtttcg tagcggtaac cggaatccat tttaggccgg
     7201 tttttgtcat ttgatttcca ttttgcagta cttcaagtaa tttccgattt tctgcggcca
     7261 acagccattc cattacttcc ttgcgtgaat ttgcccgcag atgcaaacac cgcatatttc
     7321 ccttatcccg gagatcaagc agtgaaaaaa tctgtctttc ttcgctcagg gcaacacgca
     7381 tccagccaag ttgaaattga ttcagccact ggttaaaaat agcagtctta ccattgtttt
     7441 tcgtgtttcc cttccgccac aggtcgaatt gcgtctgagc gaattttgaa agctcaactc
     7501 tattgtgctt tatttcggat tcaaccaatt ccggcaaagc taaaaacaaa tccggaactt
     7561 taaattttgc cagtcgtttt tccaaaaaat tctgtaattt agattctgaa aaatcttctc
     7621 tggtttttaa aaaagctact ggtcgtgccc cgaattctgc gtcttgtact gcaactacta
     7681 ctgcttgttc ggtctttcca aattgaagca aaattctttc tatttcttcc ggatgaatat
     7741 tttcaccacc ggaaatgaac atgctgtcct tacgtccagt aattttcaat gaaaccacat
     7801 ctccctgatt ttttgaatct ttggaacacc agagacctat atctcctgtc ttaaaccagc
     7861 cgttttcatc aaacggctga tgcagtccag ttccgtccag gtaacccaaa aaaccgattt
     7921 ttccacgcac ttcgacctca ttatctgcag caatccggac ttcgcgaaac ggcaaaacct
     7981 ggtaaaaccc gctttttccg gttgccacct gagaagccat ttctgtggaa ccgtaagtcg
     8041 tttgcacgtt cagcccaagc tgtgcggatt gttcaatcaa tgtttcagga atggatgcac
     8101 ctcccagcaa aatcaacttg agttggcata aagcgtcttt tccttttgtc gtctgcagca
     8161 agcgatgaag ttgagttgga accagagaaa ggtgtgtgac gctccgactt aggagtgtct
     8221 tggacagtga tacgttttta gcaggaatca ccattgccgc accagaaagc agagtccgga
     8281 acaaaatagc taatccgctt acatgataca tcggcagtga caacaaccaa cgatctccgg
     8341 gattcagagg cattcgctca tttgctccaa gtgcgttgta aaaatgattg gccagcgaat
     8401 gcaccactgc ttttggagaa ccggtactac cggaagtaaa aatgattgtg gcccagtctt
     8461 tcggatttat tgagtttacc tccttaccag caacagcgtt ggaatttcca acagggtttg
     8521 gttgtaacga attttcagat gtagggtgag atttgaattc gtgctgtaat tctgtgtaac
     8581 taaaagtaca gtcgattttc tgcaacatct ccattttttg tttttccggt atttgtggat
     8641 ttaatggaac cgctactgca ccgtgcatcc acaaagccaa cattgatttg agcaggtttt
     8701 caggattttc tgaacttaga tagacacgct gttgtgattc tgccatcagg gaatcactca
     8761 aaagacgtgc ttcactcatg aagtctgcaa aagtccagga acgtttttca gagatcaaaa
     8821 atggttgatt ttcgtaatgc tccagaactt gattcagtat atttggatta acgtcaaaca
     8881 tcagtccaag tctccaattt cgcaagtacc ttcggaaatt atttccaaaa aagtatcttt
     8941 ggattttggc cacgagtcag gaaaaacata attcccgttt tgtattaaaa accgaggttc
     9001 aatcaaatcc tgctcaaacc attttgaagt atccaaacct gctggaaact gttcagacaa
     9061 gagttcattt gccataaaag caatccagtt caaacccaga ccactttcaa aacaactgct
     9121 caaaacacag tggataccgt ttttttgtgc atgttcgatc cacatttttg tgttgttcca
     9181 accacccaaa ataccgggct ttaaaatcaa ggccctgatt ccggataacg atacgtgttt
     9241 ggctggggta tcagattttg gagttggagc ataccaaagt gtttcatcaa gtgccagggg
     9301 cattccagtt tgtagatgga gtttttccag tttgttactg tcaagtaacg gttcttcgca
     9361 gtattcaata ttaaaatcct gcaccgcctc tgcaaatttt acggcatcct cccattccca
     9421 ggcttggttt gcatcaaggc gcagtgctat ttcatcaccc agaatttttc gagcaagtct
     9481 gactcgctca atgtcttgca ggattgttaa ttgtccaact tttaatttta ccgccttgaa
     9541 tccaccattt cgaagttctt cacattccat ttcaagtaca gttccggaac caaccgccag
     9601 accattcact ggaaatatct gtcctgtaaa atcaattttt gagggtttgg gatcattccc
     9661 tgccgtttct ttgaaactat gaaactttga aacattggga ctttgaaaca tggttcttag
     9721 agccatgtct aaaccaaaat taacggaccc aaaaagagct gcagaacaag tgactggaag
     9781 ggaattaccg gaaagataat cccggatttg gttttcagcc tctgacagag tttccggatg
     9841 catacctggg agcggagcaa tctctccctc accataattg tcgccaactg aggtgccgtc
     9901 attttcatcg tgaagtcgaa gaatcaaacc ggtacgccca tgcatttcat gtccaagcag
     9961 gtacagaggt tctgttaatg ggagagaata atgaaaaagt ttccaacggc aaaggctcat
    10021 ttttatccaa tccaccatcc cagggaaaaa agaattccat agattaggat gagtttaccg
    10081 gtggaggcca aagttatatt cagttcactg ccttttactc cggaaataat ttgacgaatt
    10141 gaagagaagc ctggaataat tatcagtgca gaaatacaag cataccaatg tgtttccgtc
    10201 caaaaagcta aaatgaaagg aatcactgct gaaaacacaa gggctgccag atattctgct
    10261 cgagcgaagg tggtgccaaa acggacagct aaggtaagtt tcccaacttt cttatccgtt
    10321 ggttcgtcac gtaaattgtt aacggcaagg agtgctgtag ctaatagtcc gggaccgagt
    10381 cctgcaataa taactaccgg tgtaatttca agtgtttgca cataaaaggt tccgcctact
    10441 gcaacaggac caaaaaaaat tagtacaaat aaatctccaa gacccaaata accaagcggc
    10501 catggcccac ctgtgtatag tattcctgaa gcaattgaaa gtatcccgat tataacaata
    10561 ggccagccgc cccgaaaaac aagataaatt cccaccaaag tagccagtcc aaaaaccatc
    10621 acaaaattcc agagcattgt ttttggtctg acttgtcctg attgtgtcac acggacggga
    10681 ccaagacgtt cctcggtgtc tgcacccttg atatagtcaa aataatcgtt ggaaaagttc
    10741 gtgccgattt gaatcagcag tgcacccaaa agtgctgcca aggctgcaag tgcatgtccc
    10801 tttccctctc caaaagccat tgcggtgccg atcaatattg gagaaacggc tgcacccaaa
    10861 gtttttggac gaattgccga cagccagacc cgtaaataat tagaaatgtt cactacaaaa
    10921 tcttatttaa acaaattagc atggaccccg ttaagaaaac cagggacaac tcatggatag
    10981 cgggtgaatt tgttgaaatc cggtttgcgc ttttccaggt aggcattgcg tccttcctgt
    11041 ccctcctcag acatataaaa aagcatggtt gtgtttccag ctaattcctg taaaccggct
    11101 tgaccgtcac agtctgcatt aagtgccgac tttaaacagc gtaatgccat cggcgaatgc
    11161 tgcagcattt cccgtgacca ctgcagtgtt tcttcttcaa gtttttccag gggaacgact
    11221 gtgttaacca ttcccatttc caaagcttgc tgtgcagtat attggcggca taaaaaccag
    11281 atttcgcggg cctttttctg tcctaccatt cgagccagat agctggaacc ataacctccg
    11341 tcgaaggaac cgaccttagg tccggtttgc ccaaaaattg cgttatcggc tgcaatagtc
    11401 aaatcacaca tcacatgcaa aacatgtccg ccgccaatgg catatcctgc aaccattgct
    11461 acaacaggtt ttggacaggt tctgatttcc cgctgaaaat ctaaaacatt gagacgcaga
    11521 tgtccggttt cgctgtcttc ataaccggca gagccgcgta tacgttgatc acctccggaa
    11581 caaaaagctt tttcaccttc acctgttaaa attattactc cgatttttgt gtcttcccgc
    11641 gcgtcattca aggctgcacg catttcgcgg actgtcagtg gacgaaatgc attacgaact
    11701 tcaggacggt tgatggttat tttagcaata ccttcatttg atttttcgaa atttatgtct
    11761 gtgtagctgc cgcaagattt ccaataatta ttattcattt tgctccattc ttgatataat
    11821 cagtttctca aaataaaaac taatgttagc acacgtcact gttcaagacg ataaaaaacc
    11881 cagaagaaga atttttggta gaaaatttta tgaaaaagct tgatttcaag ttaacttttg
    11941 agttataatt acaggttatc atataaatca ctttatactc tgagaacagg aaaatgtttg
    12001 caattatacg tacagatgga aagcagtacc gtgtagctca aggcgaaact attcgtttgg
    12061 cccggattag tgcagaaccg ggagatgctt ttgaaacaga tcaggtgctg gccataggag
    12121 gagaagagag tgaactgaaa tttgggaaac cggtaatagt tggagcaaag gttcagggca
    12181 caatactgca gcatggaaaa gaccaaaaag ttatcgtttt taaacgtaag cgtcgaaaaa
    12241 cttatcgcag aaaatatggt caccgccagg cacacacact ggtaaaaatt gataaaatca
    12301 gtgccaaggc tcctgcagcc aaaaaatcag tgccggaaaa gataactgca gaaaaagaca
    12361 cggctaataa aactccagcc aaaaaagctg cacctgtaaa agttgctgct aaaaaggctg
    12421 cacctgtaaa agctgctgcc aaaaaggctg cacctaaaaa aactacagct aaaaaagctg
    12481 ctgacaagta acctctagcc tgtaaaaaaa caatggcaca taaaaaagct ttcggcagca
    12541 gtcgcaatgg acgtgactca caaggacaaa gacggggagt gaaaaagttt ggcggagaac
    12601 ttgttaaagc cggaaacatt ctggtacgtc aagttggttc tacttttcac gcaggtttga
    12661 atgttggtac cgggagagat tttactttgt tttcaaaggt ctctggacat gtccagttca
    12721 ttcaaaaagg aagcggaaaa cataagcgta aatatatcag cgttattgcg gacgatgcag
    12781 cagtatctgc ttctgtttga gatatgggcg ctaaaccccg ttcactttcc ggaataaaac
    12841 ctaccggtac tccccattta ggaaactatc tgggaatgat aaaaccggcg atacaattac
    12901 agaaaacgca cgagacgttt tactttatcg ccgattatca tgctcttacc tccactcgtg
    12961 atcccgagaa actaagggaa tacacaaacg atctcacagc cattttcgca gcactgggca
    13021 tggactttga gaatcacgca ttttttcgac aatctgacat tccggaagtt actgaactga
    13081 cctggatcct ttcctgtatt acatcaaagg gtatgatgga acgggcacat gcctataagg
    13141 atgcagtatc caaagagcag gatgtcagta tggggttatt ttgttatccg gttttgatgg
    13201 cttgcgatat cctgatttat gactcaaatt ttgtcccggt gggtcaggat cagcggcagc
    13261 atctcgaaat tacgcgtgac cttgccatcc gttttaatca tctttatgga gacgccttag
    13321 ttgtccccga agcaatcatt gcagacgatg ttgccaccat tccaggactg gacggaagga
    13381 aaatgagcaa atcctatgga aacataattc ctttgtttgc atcggaaaaa caatttcgca
    13441 aagctattat gaaaatcact accgactcaa aatcagtgga tgaagctaaa gatccagagg
    13501 cgtgcagtgt gtttgctctt ttcaagtgtt ttgcagataa agcagatcag gaagccttag
    13561 ccgaacgata ccgtgcagga gatatgggct atggtgaagc gaaacagctt tgcttcgaga
    13621 aacttaatgc tgaacttcat gaaccacgtg aaatttatca gcaaatacgg aacgaccagg
    13681 caaagttaga tggtatcctc gaatccggac gtgacaaagc gcgcgtcatt gccaggcaag
    13741 ttactgatcg tgtacgtaac aaagttggtt tgtgatttct gcaaaagcaa caaaaaaact
    13801 tgaattcaac tgattttcgc tgattgccgc ggataattat ctatgatttt ccgtaaaaat
    13861 ctgagttata aaacactaat ctataaccac cattcatcac attgaaatga ttcctgatat
    13921 tcttgcatca cgttatgcca gtcctgaaat cacctcaatc tggtctccag aaggaaaagt
    13981 acgtcttgaa cgagaattct ggattgcagt gatgaaggca caacgtaaat tgggagttga
    14041 tatacctgag actgcgattg cggcgtatga aaatgtccgg gataaaattg accttgaaag
    14101 catttcgcag cgtgaacaaa aactgcggca tgatgtaaaa tccagaattg aagaattttg
    14161 tgaattggcc ggatacgagc acattcacaa agggcttacc agtcgcgatt taacggataa
    14221 tgtggaacaa ctgcaaattc tccgttctct gcgtttagta agaagcaaag ctgtggcggt
    14281 attgcatcag ttggctgatt ggagcttacg caccaaagac ataatgctcg ttggacgcac
    14341 ccacaatgtt cctgcacagc cgataacttt gggcaaacgg ctggccatgt tcggagagga
    14401 aataatgttc gggcttgggc ggctcgatca cctgattgaa aattatcctt tgcgcggctt
    14461 gcagggagct gttggaacaa gacttgatca attggttttg atgaacggag ataaaaaaaa
    14521 ggtacaagag cttggtgaac aagtactgga gcatttagat tgcagtacgt ctttcaatgc
    14581 ggtgggtcag gtttatccac gcagcctgga tgtggaaacc gtgcgtgcgt tgattgcact
    14641 cagttccgga atttcgagtt ttgcgaagac tttgcgtttg atggcaggac agggtttagc
    14701 cagtgaggga tttcagcagg gacaggttgg ctcttccgca atgccccata aaatgaacgc
    14761 ccgcacttca gaacgaatca acggttttca ccaaatacta aacggttacg gcacgatgct
    14821 ttccggattg agcggtgatc agtggaatga gggggatgtt tcgtgttcag tagttcggcg
    14881 tgtggcactt cccggggctt ttttcgcgtc tgacggacaa cttgaagcgg cccttactgt
    14941 gctgagcgag atgggatttt ttgaagccgc gattgcacag gaactgcagc agtatctacc
    15001 gtttttggcc tctaccacat tgctgatgga agcagtgcgc aaaggtggtg gacgagaaag
    15061 tgtgcatgag gcgatcaagg aaaacgcggt tgccgtagcg gatgccttga ggaacggaga
    15121 tcttggtgaa aatgatttat cgcagcgact tgcagatgat gaacgtgttc ctttggattt
    15181 gcaggaaata gaagcagttc tgaatgatcc ggaacgtttt gcagcttctg caccggaaca
    15241 agcagaggca ttcgctaatc aagtacggaa atggactgaa cgctttccgg aagcaaaaaa
    15301 gctggtaccg caagtgttgc tttgagttgg gagaaagagc cggtttcgcg gcccctttta
    15361 aatatggaaa actgctggtt actcagcaga ttcgtacttc tttaactcaa ctgatttgat
    15421 atgctccaga cgtaaacgtg aatagacttc aattaaaaac ggtcgcagtt cattgatgtc
    15481 ttcgtcatca gaaaattccg tgaaaaactg ttcgtattga tccagtgcag gaacaagcaa
    15541 tccgttatcc ttcaatagcc ctgcataaag gaatccgctt tcatccatac tttgaaactc
    15601 gtctttttgc tttttgaact ttgcgagttt cttcccggat aacactttca ggttcgcagc
    15661 tttagctgtg taagccacaa cgccgcttcc atcaagaact tcaacaaaat atttttgttt
    15721 tttgctgaaa ggttttatcc tggtgcgaac aacctcatct cctgtcgccg gaacatcata
    15781 gacagcagaa tctgtccagg tattggtttt gcgatccttt gtacccaaat gaaggcggta
    15841 ggcgtaatcg gcaccggcag tttcccaggc cagctcagaa aaatctgctg aaatggtatt
    15901 tgtcgccaat ttgaggttaa tccctccttt tttagcagca cgccgcacag tagtatattt
    15961 ctgagtcttt ttgaactgct ttgacaatcc tgacaataag ccgcctccag catcggttcc
    16021 acccaggctt ccctcaagta cctctaatcc ttttttggtg aatttgattt ttgaattagc
    16081 tgtaagcaga gtagtttcgt ttgtttcttg attcaaaaac tttatcgaac tgtctgcact
    16141 gaccctgacc agataattgg cataaacgaa cttgttgcgc cgaacttttt tccagcgttt
    16201 cccctttttg ctgtattcaa ttttgccctt aatatcaacc agcattccca ggggaatttt
    16261 tttggccagt accggctgca aggcaaaggc agagagaaaa agaaataaag tgattaattt
    16321 tatgaaacgc atataacctc ctgcggttgg gattgaagtg gtttaattct agtttgaggt
    16381 taacacaaca actataaaca gacaacaccc ttttaagctg atttttattt acttcaagca
    16441 aactcggatt atgtcttttg tctccacagt ttacaagcta agattcaaca acacaaactt
    16501 ctttttttat caacggtaat tctatgataa atgtaagcaa tatacaagtt tactgattta
    16561 ttattagttg ttaccctcga tgaaaaagtt cttcagatgc aaattaatct gaattaattt
    16621 cacagatttg atgatcaggc tattgttcaa ctttgtgtga caatctctac aggcattccg
    16681 accgacagct ttccattaat tgttgcaatc agattctgac caaactgtac gttgccgttt
    16741 tgttttcgat agccagccag tgtacgcagt ggttcttttc cggaaaattt tccagtttcc
    16801 ggatcaacag tagtgagtat acaacgtgaa catgatttta ctatttgaaa ttcacattct
    16861 ccaattctga tttttttcca gttatcttct gcataaggct ccgtgttttt cacaaccata
    16921 tttggacgaa aacgtttcat tgctaaggat tccggaagac ggttgttaag gtcacttaag
    16981 gatgattcag aaatcagcag caaagggaaa ccatctgaaa acgaaacctg attttgtgca
    17041 gacgcatagt ccggatccac cggacgattg ctgtgatcag gcataaaaac aatattacag
    17101 cttttgccta aatactcact gatccacaat ttggattgtg gattggttgt ccaggcctca
    17161 caacggtcac cccaaatttc aacttcttga gattcgcctt ttgtgggaaa caaaggcaga
    17221 cttaactcgg acattcgcgg ggcgttcaaa gtcagcgttt gaccgacaag ttctgtgttt
    17281 atcaaagcca tgcttgggca ggttctttgt gtcataaatt gaccggaatc atccactacc
    17341 atccaacgtc ggtcatgtgc caatcctctt tgctccataa aagcgttatc cagagaaatt
    17401 ccacttgctg attttagcgg gtaaatattg agttgactta aaaccggatt cgtcattttt
    17461 cgatccttct acttagattc tctgtggaaa ctaaagcaag aggtatcgat gaggaaatac
    17521 cagatgaaat tttcctgaac attctctatt atcaagataa aacggcaata cgatatattc
    17581 ttcatccata acagaatcac gaagaaaccc caattgtaag atatgacggc aactggccac
    17641 ttttaatttg tcgctttttg gccatagttc taacaaactg acaaaaaatt tgcgcagcat
    17701 ttctccaatt tccagataag tgatagaatc tgcttcacgt aaatcattaa aaatatttgc
    17761 atgatttcga gaattttttg tatgaaccaa tgaagccagt ccctcaccga cggtgcggct
    17821 aaaatgcaaa atccacactg agttggaatg tttcatctca aacagagaaa aataagaccg
    17881 tccgggagac caactgctca agttggcata agacaggcat tgttcagttt cagaggtaat
    17941 tctgaaggct ccgggaagga ttgtttgaag ttgggcagtt agtgtattta ccagtctctg
    18001 aactaacaaa ttctctttag actccgagtc cctttcgatt tgctgggaaa aacctgctcc
    18061 ggggaagttt tcttcagatg ccagtttttc gattttggga acttcaaatg tctcccgaat
    18121 catttgttgc agtatttcct ctgacgtaat ttctgtgact gatgtttgat ttagcatttt
    18181 ttactttata attccaaatc tgaaatgtcc cggaaatctc tttccaattt caggatcaat
    18241 tatacatcca gattccgttt gaggccaaca caaatttttt ttaaagaaaa cttccgagtt
    18301 aataggattg aatttcctgc taatttttga gaaaatctaa ggaaaatgtt taaagacaga
    18361 ttacgtatca atttaacaga aagaaaaaaa aatcgtgaca taacattgtc tgattcaaag
    18421 ttcatgcaga agtctcttgg catcatcatt atttttctat tttgtttttc aggcggactg
    18481 ttttttgaac aaaatttagg ggcagaaagt cagggccgca aaatacaatc atatgtaaaa
    18541 aatggaactg ttttacaggc caggaaaagg gaagcacgta ttctagaaca aaaaaacaag
    18601 ggacgtaaag tgaagtatta tgttttgaaa aaatctgttt tgcaaggcag gaaaagtatt
    18661 gcaaaaatgt ctgcaaataa aatgttggaa accggtctgg ttacttctaa gatggttaaa
    18721 aataaccaat ggtttgtgaa aaagcctttt cagccgcgat atacaaaagt caatattgtc
    18781 agtaaatata ctcctccgga ggtaaatcca gaagaatctg ataaaataag ccgc
//
LOCUS       DTVD01000224           18820 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_518_length_18820_cov_379.764615, whole genome
            shotgun sequence.
ACCESSION   DTVD01000224 DTVD01000000
VERSION     DTVD01000224.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 18820)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18820)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18820
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_18820_cov_379.764615"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /gene="guaA"
                     /locus_tag="EYO68_07850"
     CDS             complement(<1..1810)
                     /gene="guaA"
                     /locus_tag="EYO68_07850"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIC07755.1"
                     /translation="MQDFIAVLDFGSQYAHLIAKRIRHLGAYTKIFSPSAKENFLQDA
                     KGIVLSGGPASVYSTDIPQFNSNILNLDLPILGLCYGHQLIAKEFGGAIANTGKGEFG
                     KAILQQTSDFPLWQNVSFPNQVWMSHQDSVTKCPPDFEVTGVTESGNVAAMRHRKRRI
                     YTLQFHPEVNDTEQGQKMLDNFINTCEICSKWSMETFIKETTERLQQEVGERKVLMFI
                     SGGVDSSVAFALLDRVLGRDQMLGLYIDNGFMRKDESTEIIERYRQLGYTNIQSRDYS
                     TFFLDAVANQVDPQIKRQKIGATFIGMRDKFLNELNLSAQEWMLGQGTLYPDIIESGG
                     TEHAEVIKSHHNRVQEVLDLVDSGNVVEPLKDLYKDEVRQVGSLLGLPDSIVWRHPFP
                     GPGLAINVLSARGDESFPNLEQTAAEVSNCLKESNCDFSILPVRSVGVQGDQRTYTSP
                     AALINTPRDWDWLEKEATRLTNEVRNINRVVLQLDSNNKDLNAPYIIRGAFCSKERLD
                     LLREADFMATQMLKENGLMEKIFQLLVILLPISKNGKEDSLVLRPVVSEDVMTAQFAR
                     INWNLLDPLVESILGLSGIETVFYDITHKPPATFGWE"
     gene            1899..2381
                     /locus_tag="EYO68_07855"
     CDS             1899..2381
                     /locus_tag="EYO68_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07756.1"
                     /translation="MFGIGLPEMIIIAVVALVFIGPDKLPGVLRSIGKGLVELKRATS
                     DVRSTVQDEMHKIEEEIELKDVRESAQNFSNELGGVANKMDPLSLSKLSSGEQLEKIA
                     DAIDKTENNDSEDQPEKEITSIKLDPRELSPEDFSTPEISTVEAKEKDSSESSVAEKT
                     "
     gene            2643..2897
                     /locus_tag="EYO68_07860"
     CDS             2643..2897
                     /locus_tag="EYO68_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07757.1"
                     /translation="MSNQEELVSELKANIDQDLKKRQKSIEDGEALDKVIHQLMDAGE
                     IGISHGTDKHVSVIMLGLATVVTLFAFYMIFGVSPDYYTG"
     gene            complement(2951..3691)
                     /gene="kdsB"
                     /locus_tag="EYO68_07865"
     CDS             complement(2951..3691)
                     /gene="kdsB"
                     /locus_tag="EYO68_07865"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIC07758.1"
                     /translation="MVKTLAIIPARYASTRFPGKPLVEIAGKSMIQHVWERAMQTPSI
                     DEVLVATDDQRILDAVEKFGAEGVLTSTEHKTGTDRIIEALSGRSCDWVLNIQGDEPL
                     VLPADLERLIQKAVKTRDTKGATLIYKITDEAELHDPNVVKVTLNQSSQALYFSRSLI
                     PFPRSPQSSNFNAWRHLGVYLFKRDFLLRFERWSQSPLEQTEQLEQLRILENGETLLC
                     VEAENDGVGVDVPQDVALAEKLMKKQRL"
     gene            complement(3737..4387)
                     /gene="tmpT"
                     /locus_tag="EYO68_07870"
     CDS             complement(3737..4387)
                     /gene="tmpT"
                     /locus_tag="EYO68_07870"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:PRK13255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HIC07759.1"
                     /translation="MFMNQWESRWQEGRIGFHLPEVNSYLLRYSDKLLTQDPESVFVP
                     LCGKTLDLPWLAGRTKKVVGIELVHKAVQDFFKENKLTHSIQQSGKLNLFSSDTIVLF
                     QGDFFDLNKEQTASFDAIYDRASIVALDQPERERYVNHLMSFLEPGGRILLITLEYDQ
                     NQMTGPPFSVPADEIERLYAPYGELELLETSDILDERFRKKGLDGMLERVFQFIKH"
     gene            complement(4411..5070)
                     /locus_tag="EYO68_07875"
     CDS             complement(4411..5070)
                     /locus_tag="EYO68_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07760.1"
                     /translation="MIIVCVSGLMLLLGGCKTIDVKGKPDLLPYMQKPVAFLTIKSPD
                     NLQKVWPELMGQVELHLKEMPTLGRVTGFKERNLKLDSNPKLRSGFRTYLSTLTLTGI
                     SEKNLAWKLEEELNSPLFLLLDFVSFPCTKECPSNVQWVIRLKLIEAHSGDLIFQVRL
                     QHKLDEDEKTAEAYNELAAKLTTKVVDEFASGFIVPWHRWRFEHLKPESVRKLRSEIG
                     I"
     gene            5275..6379
                     /locus_tag="EYO68_07880"
     CDS             join(5275..5346,5348..6379)
                     /locus_tag="EYO68_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886675.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIC07761.1"
                     /translation="MLSEEISTRIDGLTQRLDDLEGFLEVTSKRKQLQDLENETSDDG
                     FWELSQGERTVLLKQVSRLSDSIQGWETLDSLHDDLEAAAELYREEDDADLLKEILDS
                     CEKLDNDIDSLEITGLFNGEADDRNAIVSIHPGAGGTESTDWASMLYDMYRRWIAEEG
                     FKFQLLDYQPGDEAGIKSVTFSVSGPYVYGKLKTEIGVHRLVRISPFDSQKRRHTSFA
                     SVFVYAEIDEDVEVEILTDDIRVDTYRASGAGGQHVNTTDSAVRITHAPSGIVVQCQN
                     ERSQHKNKATAMKMLKAQLFDLQQKELNKEKDLQSSKKQENAWGSQIRSYVLHPYQMV
                     KDHRTNREAGNTQAVLNGDINAFIKAALSQKVC"
     gene            6419..6904
                     /locus_tag="EYO68_07885"
     CDS             6419..6904
                     /locus_tag="EYO68_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIC07762.1"
                     /translation="MASFDIVCEVDIQEVTNAVDQARREINNRYDFKGTNCEIELDKI
                     KPALMITADDELKMQTTIDILLTKLIKRSVPVKSLVYGKTEPSGRVQKKEILIQQGLS
                     KDQCKKINQFIKSTKTKVQPQTQSDSVRVSSKKKDDLQAVMQAIKGQDFDFDVQFTNY
                     R"
     gene            complement(6965..9760)
                     /locus_tag="EYO68_07890"
     CDS             complement(6965..9760)
                     /locus_tag="EYO68_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIC07763.1"
                     /translation="MRFSKLEKNTSGQESILPYKMKKNNIHFKCFVILLSLFLASCSI
                     LGSSRERGIAAVQPPPEAPLLAEVLMHLQLDYVEPEKLDPESLLQGALTELGRMIPEV
                     TVVPELQDKTLGPVLNISFKTEKTLLPVSKIAGLYDLHIALQMLMKRLLEMNPQLTQL
                     KIEQMFVRGILNQLDAYSVLLPQEIYNEFNINIGGQFAGVGLVVGTRDDQLTVIAPMD
                     GSPAALAGMKPLDRIVAVDGEKTEHMTLDEILHRLRGNIGTPVTLSVLRKGHAKALQF
                     ELLREDIQVESVETYDLSSSKQTVSYVRIKNFQIDTSRELKNKLGSLNMVDGVILDLR
                     NNPGGLLEEAIRVSDLFLPGKKRIVSTKGNVVSSVHDAKQFFAAEHLLNIPLVLLINR
                     GSASAAEIVAAALKQNERAIVIGEQSFGKGTVQTLWDLKDGSGLKLTIGEYLTPSGRS
                     IHNIGVMPNLRLIPVTVPELKNGETELGRQEIFTTEKRFGLLSDFDAENRVNDLDELS
                     ISYLSHTPILNEDSEIIDNNLKIEKLKADIFVKTAEQLLSQWNPKNTNSVIQKISREA
                     EQKESEIITEALSKHGIDWSLNPFIKSATPEMLILTWSAEMISTDLLRVKVNLRNSGK
                     IDAQRLIAVTRASNGILDSLEFPIGKLVSEENTSRTIDVKIPAGMMEEQEPVEILLFD
                     QNLKKLKSVRRQLHFSPKRNHSFWLRMKIFDNGRFGSRGNGDGRVQTGETIALAFKIE
                     NKSKSTVPELLLKLRGTEGAFRINRGKLLLKNLEPHKKQKDYFLFKSLMDSGTLGKIS
                     LEMVDIKAGTPGIVYLWNLNNMFPEQKLVTPELLGVSWQDKDGNYVESETDLSSLILQ
                     GNVSNVNDVRDVYVYLNNKKVFYSLNRNSGQQDKNTEFHFQTEVNLDTGNNQISIFSR
                     SRSGFTSERRLRIFRKQ"
     gene            9869..11413
                     /locus_tag="EYO68_07895"
     CDS             9869..11413
                     /locus_tag="EYO68_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HIC07764.1"
                     /translation="MNIGSLLPRHARYRSKHLAFVIGEERLNFCELNIRVNRLANALL
                     AAGILKGQKMATVLPNCEELMLLYWAAAKTGIVIVPGSPLLQASGLTTLLRDSDTEIV
                     FADASFAETLQSIKKDLPAIQNMHWILVGDGKRHSSGLTVSGFKSYADFISGAEETEP
                     PDAKIVDGDIYNIMYSSGTTGAPKGIIHTHYVRANYCTHFASAWRMSPESIVLHAGAI
                     VFNGAMLDLMPWMYLGATYILHHSFDAGAVLKTIEEEKVTHIVMVPAQIMALLNHPEF
                     MPEKLGSLEMLQNVGAPLLLEYKHKLNEVLPGIFYELYGVTEGFFSVLDRDDALRKVG
                     SVGSAPSFIDIRILRENGEECEPDEVGEICGRGPMMMPGYYKRPDLTKKTIVDGWLHS
                     GDLGFIDEDGFLFLVDRVKDMIISGGVNVYPKDIEEVIIQHPAVSEVAIFGIPDEKWG
                     EIPIAAVIFHKEQNVVEEDLKTWINQRVDAKFQRVADVQVLEDFPRNIAGKTLKREMR
                     EIYVNN"
     gene            11597..12904
                     /gene="hflX"
                     /locus_tag="EYO68_07900"
     CDS             11597..12904
                     /gene="hflX"
                     /locus_tag="EYO68_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIC07765.1"
                     /translation="MSDSRLFKNWDRALLVSLQLPHRSNSEVQSSLDELSSLVYSIGG
                     KVTGQIVQARTQIHPAYYFGKGKLTEIKSRIHIDEVDAVLVDNPLSPKQTQNLEKLLD
                     CEVLDRTQVILDIFAKNARTSEAKLQIGLAQSEYLLPRLVGLWKHLDRERGGIGASRG
                     TGEKQIEKDRQFLRQRITRYKSQLIRVEKERNTQKQRRSNCLQVSLIGYTNAGKSTIM
                     NALTNSGLLVEDRLFATLDSTTRLLEEDSRPKVLLSDTVGFISNLPHEVIAAFRSTLA
                     TVRDADLLLQVIDADDNIEEHLQTTSEVLEDLDAACIPIIKVFNKIDRISPTRLLILE
                     KSFPEAVFGSCENGGKNGLEKNSAFVEQLRKQILLFFNERMKTMTIRLDYQHSQKLAN
                     IYELSRVDNIDYQEEGILMTLTAIPGNLERLRHHLGSDYTEMR"
     gene            13062..14348
                     /gene="murA"
                     /locus_tag="EYO68_07905"
     CDS             13062..14348
                     /gene="murA"
                     /locus_tag="EYO68_07905"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIC07766.1"
                     /translation="MEKFVVHGGIPLSGSITPSGNKNEALPVLAATLLAQEPVILHNV
                     PDILDITIMRGLLESLGVEVKQIDKHSFSFDPGNLQSANPDSTQASRIRGSFLLAGPL
                     LARKKSIVLPAPGGDRIGLRPVQTHLSALEKLGARIDLNENGFYQMQTSGLKGTQIYL
                     DEASVMATENAVMAAVTAQGKTNIYNAACEPHVQGLCHFLKLLGANIEGIGSNLLVIE
                     GVSGLSGGEYTIQPDHTEVGSFIGLAAVTGSELRIRNAGVQYLRMTQLMFEKLGVEIQ
                     VEGDDLLIPKNQKLIVRKDLSGGIPKIDDAPWPGFPADLTSIMTVVATQCSGAVLIFE
                     KMFESRLFWVDKLIEMGAQIILCDPHRAVVSGPTPLKGARVSSPDIRAGMALLIAALG
                     AKGITEIHNIDQIDRGYETIDKRLNALGAKIMRVSE"
     gene            14357..16804
                     /locus_tag="EYO68_07910"
     CDS             14357..16804
                     /locus_tag="EYO68_07910"
                     /inference="COORDINATES: protein motif:HMM:PF00580.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07767.1"
                     /translation="MKKNKNLSPEQAKVLEEEQQLLLLVKQGLLMFAAEDKERNISNL
                     HLDNIIELRDSLTDTLPEDVPAVMAQMERMVLLHSQQDSHNAAFGFNQDVPYFAHIRL
                     RENKRERDLLIGNQNCFSTHLPCPIVDWKNAPISQIFYRYHEGDEYVEDIGESELEGE
                     LITRRMLLIENGILMRINWPGGVLEDLSTEPTKHNKWHLVSHKTPQLETGQDKTLDEL
                     QPPDKYTSSNGKTTDLNFSGYRVDKHLRQITALVDPEQFEVITHSEYGIVLIQGGAGS
                     GKTTVALHRLAYLMSKKPQYFKPKTVLPIVFGPALANYMSRVLPALGVHGARPRTYHK
                     WLSGLRIRMLPELPVQYAENTPMDVIELKRHPLWLKYYREEIQNRTTKFRELFEDKLA
                     KVEGLEILLKAWDAMSDTALIPRLQNLAGWSKGEETIQGVPQLSSARMEKQLQNALEQ
                     AFPELMESPHLLTTQIWNDCLVNRELLFETVERLAPGEFNESQLTGVWSWAVRQYQKR
                     QESKEIVESDLVSLTEQTEDFFNEDLNFTDEKSSLDEEDDTLLLLLYQLLLGPLNRKN
                     GKPLQYTHLMLDEAQDFGSLEFQLLVSLTPENLPSVTLAGDLDQRIMVGRKHETWEES
                     LGHLTLPDGSKPDVTALAPLKIGYRSTHEIMTVAKKVIGEHSVNTVWYSTRHGAPVDV
                     FRFKERGAMVAFLADTLKDLSIREPLASVAVLTRTQESAEKLFDGLQRTDLSQLRLIR
                     DQEFTFTPGIEVTDITQTKGLEFDSVIIADADASTYGPDIRSRQLLYVGVTRAAHQLW
                     LLHCGNPSSLITGILDK"
     gene            16907..18172
                     /locus_tag="EYO68_07915"
     CDS             16907..18172
                     /locus_tag="EYO68_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC07768.1"
                     /translation="MQPVINNQIGSLKESATLFINQLALKLRSEGQNICHLGFGESPF
                     PVPEPMRIALQENAYRKQYISGYGLPELRKAVAGFFKSEFGYNYSFEKIFIGPGSKEL
                     IFQLVYLLEGPLMVPAPSWVSYGPQAKIRSKTFIPIPTDLKNGYRLQAEELEKTCSGL
                     SFPQKLLILNNPSNPTGSVHTPEELKDLAEVCRKHGIIVISDEIYALTNFGEQPFEGI
                     AKYYPEGTITTSGVSKAFSAGGWRLGFAMIPDELAKIVPPLSAFVSETFSCVSAPVQY
                     GALSGFENYDSVRKHVERCRDIHCAAGHFLHQRFTEIGLNCPKPQGAFYLFPDFENFK
                     EKLSSHGITTSEKLSRRLLEETGVALLPGSDFYMPNDFLVVRVASVDYDGAQLLKNFP
                     EENQLTPEMFPNLFPKLADAAERIEKWLS"
     gene            18413..18745
                     /locus_tag="EYO68_07920"
     CDS             18413..18745
                     /locus_tag="EYO68_07920"
                     /inference="COORDINATES: protein motif:HMM:PF04519.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07769.1"
                     /translation="MKSVKGYLGSDVRMDGSLETNDSLRIDATFVGNVSSSHSVVVGT
                     RGRVMGKIQAPVIKVDGWVEGDLKAGKLVEVLGNARIKGNIYTPVGGLKMRLGGEFKG
                     KFIMHFTK"
BASE COUNT         5686 a         3796 c         3891 g         5447 t
ORIGIN      
        1 attcccagcc aaaagtggca ggaggcttat gagttatatc gtagaaaact gtttcaattc
       61 cagatagtcc aagtattgat tctaccagag gatcaagcaa attccaattg attcgagcaa
      121 attgagcagt catcacatct tcagaaacaa cgggacgcaa gaccagactg tcttcttttc
      181 catttttaga tattggcagc aaaattacca gcagttgaaa tattttttcc atcaaaccat
      241 tttctttcag catctgtgtt gccataaaat ctgcctctct cagcaagtcc agtctctctt
      301 tgctgcaaaa agctccacga attatatatg gtgcattcag gtccttgttg tttgaatcaa
      361 gttgcagtac aacccggttg atgttccgga cctcatttgt taaacgggtt gcttcttttt
      421 ccaaccagtc ccaatcgcga ggggtattaa ttagtgcagc aggagaagtg taagtccgct
      481 ggtcaccctg aactccaaca gatcgaactg gaagaattga aaagtcacaa ttgctttcct
      541 tcaaacaatt cgacacttcg gctgccgttt gttcaagatt aggaaacgac tcatcaccgc
      601 gagcagataa aacattaatc gcaagtcccg gtccaggaaa tggatgacgc cagacaatcg
      661 aatccggtaa gcccaacagg cttccaactt gccgtacctc atctttgtaa agatctttga
      721 gaggctccac aacatttccg gaatcaacca aatcaagaac ttcctgaaca cgattgtggt
      781 gtgatttaat gacttcggcg tgttcggttc ctccagactc aataatatca ggatagagtg
      841 taccctgccc tagcatccat tcctgtgcag ataaatttaa ttcattcaaa aacttgtcac
      901 gcatcccgat gaaagttgca cctatttttt gacgctttat ttgtggatca acctgatttg
      961 ctactgcatc caaaaaaaaa gtactataat ctcttgactg aatattagtg tagcccaact
     1021 gccgatatcg ctcaataatt tcagtacttt catcttttcg cataaagccg ttatcaatat
     1081 aaagccccag catttgatct cgtccaagca ctctgtctaa caaggcaaat gccactgatg
     1141 aatccacacc cccactgata aacatcaaga ctttccgctc tccaacttcc tgctgcagcc
     1201 gttccgttgt ttccttgata aaagtttcca ttgaccactt ggagcaaatt tcacaagtgt
     1261 taatgaaatt gtccagcatt ttttgtccct gctcagtatc gtttacttcc ggatgaaatt
     1321 gcagagtata aatccgacgc ttacgatgtc tcatagcggc cacgtttccg gattctgtca
     1381 ctcctgtaac ttcaaaatcc gggggacatt ttgttacact gtcctggtga ctcatccaaa
     1441 cctggtttgg aaatgaaaca ttctgccaaa gtggaaaatc tgaagtttgc tgtaaaatag
     1501 ctttaccgaa ctctcctttt cctgtgttcg caattgcacc tccaaactct ttggcaatca
     1561 attgatgtcc gtaacacaaa ccaagaattg gtaaatcaag atttaaaata tttgaattaa
     1621 attgtggaat atcagttgag taaactgatg ccggccctcc tgacaaaact attccttttg
     1681 catcctggag aaaattttct tttgctgatg gagaaaatat tttagtatag gcacctaaat
     1741 gccttatccg ttttgcgatc agatgtgcat actgacttcc aaaatccaga accgcgatga
     1801 agtcctgcat tgcttttcct ttctgagcag gttaatctaa ttgaagacat atcctaacag
     1861 tatctgaaaa tttgcacaat ctcacataga gtactcacat gtttggaatc ggactcccgg
     1921 aaatgataat tatcgcagtt gttgcactgg tatttatagg gccggacaaa cttccaggtg
     1981 ttttaagatc aattggaaag ggtttggtgg agctaaaacg cgccacaagc gacgtacgct
     2041 ccactgtaca ggatgaaatg cacaaaattg aggaggaaat tgagctcaag gatgtgcggg
     2101 aatctgctca gaattttagt aatgagcttg gaggagtagc aaataagatg gacccgttgt
     2161 ctctctcgaa attgagttcg ggcgaacaac tggaaaaaat tgcagatgca attgacaaaa
     2221 ctgaaaataa tgattcagaa gatcagcctg agaaagaaat cacatcaata aaattagacc
     2281 ctagggagct ttctccggaa gatttttcta ctcctgaaat cagtacggtg gaggcaaaag
     2341 aaaaggattc ttctgaaagt tctgttgcag aaaaaacata aaataccacc gaacaatatt
     2401 aatcaaaact taatcttgat gccatcgcaa aaagtcctat aaccgtttca taaagtggtt
     2461 ttcagaccta ttgcgagacc gtcaatactt tatgcattaa caaaaagctt ggctgaaatt
     2521 tttatctatg ctagcgttga tgttcggtta ataatttatt gttgactctg ccacggtcac
     2581 atttggaaca gtcataatca ggcagtttcg tttaatttcc aaatatttta aaaaggtgag
     2641 atatgagcaa ccaggaagag ttggttagtg aattaaaggc aaacattgat caagatttaa
     2701 agaaaagaca gaaaagtatt gaagacggtg aggcacttga taaagtcatt catcagctca
     2761 tggatgccgg agaaattggt atttcccatg gaaccgacaa gcatgtaagc gtcataatgc
     2821 ttggcctggc aacggtggtg acactatttg ctttttatat gatttttggg gtcagcccgg
     2881 actattacac aggttgaatc aagtctgaaa aaatttaaat ctataatcct gaaaaaatag
     2941 atccctcttt ttaaagtctt tgtttcttca ttaatttttc tgcaagcgct acatcctggg
     3001 gaacgtcaac tcctacacca tcgttttcgg cttccacaca aagcaaagtt tctccgtttt
     3061 ccaatattct caattgttct aattgttcag tctgttccag cggtgactga gaccaccgtt
     3121 caaatcggag aagaaaatcc cttttgaaca aatagacacc caaatgacgc caagcattaa
     3181 aattactgga ttgtggagaa cggggaaatg gtatcaacga acgtgaaaaa tacaaagcct
     3241 ggctgctttg atttaatgta actttaacca catttggatc gtgcagttct gcttcatctg
     3301 tgattttata aataagagta gcacctttgg tatcccttgt tttcacagct ttctgaatta
     3361 gtcgttcaag gtcggcaggc aaaaccagtg gctcatcacc ttgaatattc agtacccagt
     3421 cacaggatct tccggaaagc gcttcaataa ttctgtctgt accagtttta tgttctgtac
     3481 tggtcagcac tccctctgct ccaaattttt caactgcatc cagaatacgt tgatcatctg
     3541 ttgcaaccag tacttcatca attgatggag tctgcatggc acgttcccag acatgctgaa
     3601 tcattgattt tccagcaatt tctactaatg gcttacccgg gaacctggtg gaagcatagc
     3661 gtgcgggaat aatagctaaa gtttttacca atgtagatgt gctcacaaac gttcttgcaa
     3721 ttaaagaaaa tattaattaa tgctttataa actgaaagac tcgttccagc attccatcca
     3781 gacccttttt acgaaaacgc tcatcaagaa tgtcgctggt ttcaagaagt tccaattcac
     3841 catacggagc ataaagcctt tcaatttcat cagcaggtac actaaaagga gggcctgtca
     3901 tttggttttg atcatactcc agagtaatga gtaaaattcg tcctcccggt tcaagaaatg
     3961 acattaggtg atttacatat ctttccctct caggctgatc aagcgcaaca atcgatgctc
     4021 tgtcataaat agcatcaaat gaggccgtct gttccttgtt taagtcaaaa aagtcacctt
     4081 gaaaaaggac tatggtatca ctactgaaaa gatttaattt tccagactgc tgaatcgaat
     4141 gagttagttt attttcctta aaaaaatctt gaactgcttt gtgaaccaat tcaataccaa
     4201 ccactttttt ggttcttcct gccagccaag gcaagtcaag ggtttttccg caaagtggaa
     4261 caaaaacact ttccggatct tgcgttaaca atttgtcaga ataacgcaac aaatacgaat
     4321 ttacttccgg aagatgaaag ccgattcgac cttcttgcca gcgtgactcc cattgattca
     4381 taaacattaa tgcttagaaa agatgaattg ctagatgcca atttcactgc gtaatttgcg
     4441 cacagattct ggcttcaaat gttcaaaacg ccagcgatgc caaggcacta tgaatcctga
     4501 agcaaattca tctactacct ttgtagtaag ttttgcggct aattcattgt atgcttcagc
     4561 agttttttca tcttcatcca gcttgtgctg caatctaacc tgaaaaatca gatctccgct
     4621 atgagcttca atcagcttta gacggattac ccattgtaca ttagaggggc actctttagt
     4681 gcagggaaat gacacaaaat caagtagtaa aaaaagagga ctattcagtt cctcttcaag
     4741 tttccaggca agattttttt ctgaaattcc agtgagtgtc aaagtgctga ggtaagtcct
     4801 gaatcctgag cgaagtttag gattactatc caatttaaga tttcgctctt tgaaacctgt
     4861 tactctgccc agagtcggca tctcctttaa gtgtagctcc acctggccca tcaattccgg
     4921 ccagactttt tgcaggttat ctggtgactt gattgtcaaa aaagccacag gtttttgcat
     4981 atagggtaaa aggtccggtt tgccttttac gtcaattgtt ttacaccccc caagaagcaa
     5041 catcagtccg gaaacacaga caattatcat aagaattgaa aatgaaaaac aaattctaat
     5101 cgtttttatt accatgtcaa aaaatgattt atcaaaaagt tttttattag actaatccac
     5161 catactggcc tgcagattaa acacaaggtt taaagattta aacttgttat ttcaaatata
     5221 taatcttatc atttaatgtc caaatattac tataattgaa aaggatttga tccaatgttg
     5281 agcgaagaaa tcagtacgag aattgatggt ctgactcaga ggctggacga cttagaaggg
     5341 tttctttgaa gtcacttcaa aacggaaaca attgcaggat ctggaaaatg agacttccga
     5401 tgatggtttt tgggaattgt cccagggaga gcggactgtt ctcctgaagc aggtttcgcg
     5461 cttgagtgat tccatccagg gttgggaaac attggactcc ctgcatgatg atttggaagc
     5521 tgctgcagaa ttgtaccggg aagaagacga tgcagatcta ctgaaagaaa tccttgattc
     5581 ttgtgaaaaa cttgataatg atatcgattc tctggagata accggtctct ttaatggtga
     5641 ggcggacgac agaaatgcca tcgtctcaat tcatccgggt gcaggaggaa ctgaatccac
     5701 agactgggcg tcaatgctct acgacatgta ccgcagatgg atcgcagagg aaggatttaa
     5761 atttcagctg ctggactatc aacctggcga tgaagccgga attaagtcag taactttttc
     5821 tgtttccgga ccctatgttt atggtaaatt aaaaactgaa attggagttc atcgactggt
     5881 ccgaatttct ccttttgatt cacaaaaacg ccggcacact tcgtttgcat ctgtctttgt
     5941 atatgctgaa atcgatgaag atgtagaagt tgaaatcctg actgacgata ttcgggttga
     6001 cacctaccgt gcaagtggtg caggaggtca gcatgtaaat actaccgact ctgctgttag
     6061 aataacacat gctcccagcg gaattgtggt gcaatgtcaa aacgagcgct cacagcataa
     6121 aaacaaggcc actgcaatga aaatgctcaa ggcccagctt ttcgatttac agcaaaagga
     6181 gttgaacaaa gaaaaggatt tgcagagcag caaaaagcag gaaaatgcct ggggcagcca
     6241 aatcaggagc tatgttctgc acccatatca gatggtaaag gatcatcgta caaaccgaga
     6301 ggcaggaaac acacaagcag ttctcaacgg tgacataaat gctttcataa aagccgcact
     6361 ttcccagaag gtgtgttaac agacagctaa actaataacc caaaccaaca actagaaaat
     6421 ggcctcattt gatattgtct gcgaagttga catccaggaa gttacaaatg ccgttgatca
     6481 ggcacgaagg gaaatcaata accgttatga tttcaaaggt acaaattgtg aaattgagct
     6541 ggacaaaata aaacctgctt taatgatcac tgcagacgat gagttgaaaa tgcagacaac
     6601 catcgatatt ttgcttacaa aactgattaa acgctcagta cctgtaaagt cactggttta
     6661 cggaaaaact gaacccagcg gcagagttca aaaaaaagaa attttaatcc aacaaggttt
     6721 gtccaaggat cagtgcaaaa aaattaatca atttatcaaa tcaaccaaaa caaaagttca
     6781 accacaaact caaagtgatt ctgtaagagt gagcagcaaa aagaaagatg acctgcaggc
     6841 agtgatgcag gctatcaagg gacaggattt tgactttgac gtacagttca caaattaccg
     6901 ttgataaaat actgttgcag ttaatttttc agttacatta gcgttttttt gaacattcta
     6961 ctcttcattg cttccggaat attcttaatc tgcgttcaga ggtaaaacct gagcgactcc
     7021 gtgaaaatat tgagatttga ttatttccgg tatccaaatt tacttcagtt tgaaagtgga
     7081 attctgtgtt tttgtcttgt tgacctgagt ttctgttcaa agaataaaac acttttttat
     7141 tatttaagta aacataaaca tctcgtacat cattaacatt gcttacattc ccctgtaaaa
     7201 tcaaggaact caaatcagtt tcagattcca cataatttcc gtctttgtct tgccaactga
     7261 ctcccagtag ctccggtgtg acaagtttct gttccggaaa catattgttc aaattccaaa
     7321 ggtagacaat tcctggagta ccagctttaa tatcaaccat ctccaggctg atttttccta
     7381 aagtcccgga gtccattaaa ctcttaaaaa gaaaatagtc tttttgtttt ttatgaggtt
     7441 cgagattttt caataaaagt tttcctcgat taattctaaa tgcaccctca gttcctcgca
     7501 gtttcagcaa caattcagga acagtacttt tgcttttatt ttcaatttta aatgcaagtg
     7561 cgatggtttc tccggtttga actcttccgt caccattacc acgactgcca aacctcccat
     7621 tatcaaatat tttcattctc aaccaaaaag aatgattccg tttcggtgaa aaatgcagtt
     7681 gccttcgtac tgattttaat tttttcaagt tttgatcaaa aagcaggatt tctaccggtt
     7741 cttgctcctc catcattccc gcaggaattt taacgtcaat ggtgcgggat gtattttctt
     7801 cagacacaag ttttccgatg ggaaactcca ggctatccag tatcccgtta ctggcccgtg
     7861 ttaccgcaat caaacgttgc gcgtcaattt taccagaatt ccggagatta actttcaccc
     7921 gcaaaagatc tgtagaaatc atttccgcag accaggttaa aatcagcatt tccggagttg
     7981 ctgattttat gaacggattc aaactccaat caattccgtg ctttgacagt gcttctgtaa
     8041 ttatttcaga ttctttttgc tctgcttccc tggatatttt ctgaataaca gagtttgtat
     8101 ttttaggatt ccactgagat agtaactgtt ctgcagtttt gacaaatata tctgctttta
     8161 atttttctat cttcaaatta ttatcaatta tttcagaatc ttcattcaga attggagtat
     8221 gtgacaaata actgatactg agctcatcta aatcatttac cctgttttct gcatcaaaat
     8281 ctgaaagcag accgaaccgt ttttcggtag tgaaaatttc ctgcctgcca agctctgttt
     8341 ccccgttctt gagctctgga acagtaacag gaataagccg cagattcggc atgacaccta
     8401 tattgtgaat tgagcgtccg gaaggtgtta aatactctcc tatggttaat ttcagtcctg
     8461 aaccgtcttt taaatcccaa agtgtttgaa ctgtaccttt gccaaaactt tgttcgccaa
     8521 tcacaattgc acgctcattt tgttttaagg cagcagcaac aatttctgca gcagaagcgc
     8581 tccctcgatt tatcagcaaa accagtggaa tgttcaataa atgttctgcg gcaaaaaatt
     8641 gttttgcgtc atggactgag gaaacgacat tcccttttgt gcttacgatg cgttttttcc
     8701 ccggcaaaaa caaatcactg actcgaattg cttcttccag caatcctccg ggattattgc
     8761 gcaaatccaa aatcactcca tcaaccatgt tcaaagaacc aagcttattt ttaagttccc
     8821 gggatgtgtc aatttgaaaa ttttttatac gaacataact aacagtttgc ttgctgcttg
     8881 acaaatcata tgtttcgacg ctttcgacct gaatatcttc acgtaagagt tcaaactgca
     8941 aagcctttgc atgtccctta cgtaaaacac tgagcgtaac aggtgttcca atatttcccc
     9001 gcagacgatg aaggatttca tcaagggtca tgtgttcagt tttttctccg tcaactgcca
     9061 caattctgtc aagcggcttc attccggcaa gtgctgcggg actgccgtcc attggtgcaa
     9121 ttacagtcag ttgatcatct cgtgttccaa caaccagacc aactccggca aattgaccac
     9181 caatattgat gttgaattca ttataaattt cctgcggcag caaaacagaa taggcgtcta
     9241 actgattcaa tatcccacga acgaacatct gctctatttt taactgtgtc agttgaggat
     9301 tcatttctaa aaggcgtttc atcagcattt gcaaggcaat gtgcaaatca tacagccctg
     9361 caatttttga aaccggtaag agagtttttt cggttttaaa acttatgttc aaaactggac
     9421 ccaaagtttt atcctgcaat tcaggtacaa ctgttacttc cggaatcatc cgcccaagtt
     9481 ctgtcaatgc gccttgaagc aagctttcag gatcaagttt ttctggctca acataatcca
     9541 attgcagatg catcagcact tctgccagca aaggagcttc tggtggaggc tgcactgcag
     9601 ctatccccct ttcccgtgaa cttcccaaaa tactgcagct tgccaaaaac agtgacagca
     9661 gaattacgaa acatttgaaa tgtatgttat tctttttcat tttgtagggc agtattgatt
     9721 cttgtcctga agtgtttttt tcaagtttac taaacctcat attgatgatt ccagctttta
     9781 cttgaaagtg caaagagtct atgttattta tcacatcact ataaattacc tgttagatat
     9841 tttttttaac acagaaaata ataaacaaat gaacattgga tctttactgc caagacatgc
     9901 ccgttaccgt tccaagcacc ttgcctttgt tatcggtgag gaacgattaa acttctgcga
     9961 acttaatatt cgtgtaaaca ggctggctaa tgccttgctg gctgccggca ttctaaaggg
    10021 gcaaaaaatg gcgacggttc tgccaaactg tgaagaattg atgctgcttt actgggctgc
    10081 agccaaaacc ggaatcgtga ttgtaccagg cagtccttta ctgcaggctt ccggattaac
    10141 gacactgctc cgtgattctg atacagagat tgtttttgca gatgcatcct ttgcagaaac
    10201 attgcagagc ataaaaaaag atttaccagc tattcaaaat atgcattgga tactcgttgg
    10261 tgacggcaaa aggcacagca gtggacttac agtttccgga tttaagtctt atgcggattt
    10321 tatttccgga gcagaagaaa cggagccgcc ggatgccaaa atagtggatg gtgatatcta
    10381 caacatcatg tattcaagtg gtacaaccgg tgcgcctaaa gggattattc acactcatta
    10441 tgtccgtgcg aattactgca cccatttcgc ctcagcctgg cgtatgtctc cggaaagcat
    10501 tgtgctgcat gccggagcga ttgtttttaa cggagcaatg ctggatttga tgccatggat
    10561 gtatttggga gcaacttata ttttacacca cagctttgat gcaggagcag ttttaaagac
    10621 aattgaggaa gaaaaagtaa cgcacatcgt catggtaccc gcacaaataa tggccctgct
    10681 caatcatccg gaatttatgc cggaaaaact gggttcgctg gaaatgcttc agaacgtagg
    10741 tgctccattg ctgctggaat acaaacacaa attgaatgaa gtactgccgg gaatatttta
    10801 cgaattgtac ggtgtcactg aaggtttctt ttccgtattg gatcgtgacg atgccctgcg
    10861 taaagtcgga tctgtcggat ctgccccatc atttatcgac atcagaattc tgcgtgaaaa
    10921 cggcgaagaa tgtgaacctg atgaggttgg agaaatctgt ggtcggggtc caatgatgat
    10981 gcccggatat tacaaacgtc cggatttaac aaaaaaaact attgttgacg gctggcttca
    11041 ttccggagat ttgggtttta ttgatgagga cggtttcctt ttcctggtgg atcgtgttaa
    11101 agacatgatc atcagcggag gtgtcaatgt atatcccaaa gatattgagg aagtaataat
    11161 ccaacatccg gcggtttcag aagttgcaat atttggaatt cctgatgaaa aatggggaga
    11221 aatcccgatt gccgctgtca tttttcataa agagcaaaat gttgttgaag aggatttaaa
    11281 aacctggatc aatcaacgtg tggacgcaaa atttcagcgt gtggctgatg tacaagtact
    11341 tgaagatttt ccgcgaaata ttgccggtaa aacactaaag cgtgaaatgc gtgaaatcta
    11401 tgttaacaac tgattcttta cacattagaa tttcagtggg ctttctcact gaattctaag
    11461 tagatggaat gctgatcata attattggta accctgttga tttcgcgcaa aagatttcat
    11521 tgtacattta ccctaattat atgctacaat tctgcccgat aatataacac tactttagtc
    11581 aatatcctga ggttatttga gcgatagcag attatttaaa aattgggacc gcgcattact
    11641 tgtttcgctg cagttaccac atagatcaaa ttcagaggtc cagagttcac tggatgagct
    11701 ctcatcactt gtctattcta tcggtgggaa agtcaccgga caaatcgttc aggcccgcac
    11761 acaaatacat ccagcatatt attttggaaa gggtaagctc actgaaatta aatccagaat
    11821 acacattgat gaggtggatg cagtacttgt tgacaatcca cttagtccta aacaaacaca
    11881 aaatttagaa aaattattgg actgcgaagt gctggatcgt acccaggtaa ttttagatat
    11941 ttttgcaaaa aatgcccgta ccagtgaggc aaagctgcaa ataggcttgg ctcagtctga
    12001 atacttatta ccacgtttag tgggtttgtg gaaacatctt gaccgggaac gtggaggaat
    12061 tggcgcatca agaggaacag gtgaaaagca gattgaaaaa gacagacaat ttttgcgcca
    12121 gcgaattact cgttacaaaa gtcagttaat acgtgtagaa aaggaacgca acactcagaa
    12181 gcaaagacgc tccaattgtt tgcaggtgag tttgatcggc tacaccaatg ccggaaagtc
    12241 cactataatg aatgcgctca ccaattccgg tttgttagtt gaagaccgtt tgtttgccac
    12301 gctcgattcg acaacgcgtc ttttagaaga ggattctcga cccaaagttt tgctttcgga
    12361 taccgttggt ttcatcagta atcttccaca tgaagtaata gcagcttttc gttccacatt
    12421 agccaccgtg agagatgcag atctgctgct gcaggttatt gatgccgacg ataacatcga
    12481 agaacatttg caaacaacct ctgaggtttt ggaagatttg gacgcagcct gtattccaat
    12541 tattaaagta tttaacaaaa tagaccggat ctctcctaca cgtttactga tcttggagaa
    12601 atcttttccg gaagcagtat ttggttcctg cgaaaatgga ggaaaaaacg gattggaaaa
    12661 aaattcagct tttgttgaac aattaagaaa acagatactg ctatttttta atgaacgaat
    12721 gaaaacaatg acaattcgtc tggattatca gcactcacag aagttagcta atatttacga
    12781 attgagcagg gtggacaaca ttgattacca ggaggagggc attttaatga cactaacagc
    12841 aattcccggt aatttggagc gtttacgaca tcacttagga agtgactaca cagaaatgag
    12901 ataaactgtt cgaggtttgg gtttttaaca aaggagtttt aacaaataat agtatgtacc
    12961 ttattctgcg taacttacgt ggactgttaa tagctgactt tctggacgtt caatcaaaac
    13021 attaaaagac tttaaaaact cttatattta aataaattta aatggaaaaa tttgtagtgc
    13081 atggtggaat tcctctctct ggaagcatca ctccttcagg caacaaaaac gaggctctcc
    13141 ctgttcttgc tgcaacttta ctggcacaag aacctgttat tttacataac gttccagaca
    13201 ttcttgacat cactattatg cgcggactgt tggaaagtct aggtgtagaa gtaaagcaaa
    13261 ttgataaaca ttcatttagt tttgatcccg gtaatcttca atctgccaat cctgattcaa
    13321 ctcaggcctc taggattcgt ggatcgttcc tccttgcagg gccccttctt gcccgcaaga
    13381 aatcaattgt tttgcctgct ccagggggag acagaattgg tttacgacct gtgcaaaccc
    13441 atctttcggc actggaaaag cttggtgcca gaattgatct aaatgaaaat gggttttatc
    13501 agatgcagac ctctggttta aaaggaacac aaatttatct tgatgaggcc agcgtcatgg
    13561 ctacagaaaa tgcggtcatg gctgcagtta cagcacaggg aaaaacaaat atttacaatg
    13621 cggcttgtga accacatgtt cagggactgt gtcatttcct gaagttgctg ggagcaaata
    13681 ttgaaggtat aggaagtaat ttactggtca ttgaaggcgt gtccggattg agtggaggag
    13741 agtacacgat tcagccggat cacactgaag ttggttcttt cattggattg gccgctgtta
    13801 ctggaagtga attgcgcatc aggaatgccg gagtgcaata tttacgtatg actcaactaa
    13861 tgtttgaaaa actgggagta gaaattcagg ttgaaggcga cgatttgctt ataccaaaaa
    13921 atcaaaaact catcgtcagg aaagatctaa gtggaggaat cccaaaaatt gatgatgcac
    13981 cctggccggg ttttccagca gatttaacca gtattatgac agtagttgcc acacaatgtt
    14041 ccggtgcggt tttgattttt gaaaaaatgt ttgaaagccg tttgttttgg gtagacaagc
    14101 tgatagaaat gggagcacaa attattctgt gtgatccgca tcgagcagtt gtttcaggac
    14161 ctacgccact caaaggggca agggtttctt ctccggatat tcgggcagga atggctttat
    14221 tgattgcagc acttggtgca aaaggcatta cggaaattca taatattgac cagattgacc
    14281 gtggatatga aacaattgac aaacggctta atgctctggg cgccaaaatt atgagagtaa
    14341 gcgagtagtg gaaaaaatga aaaaaaataa gaatttatcc ccggagcaag caaaggtttt
    14401 ggaggaagaa caacaacttc tgctccttgt aaaacaaggg ctgcttatgt ttgctgctga
    14461 agataaagaa aggaatataa gtaacctgca tttggataat atcattgagt tgcgggattc
    14521 ccttacagat actttgcccg aagatgtgcc tgcggttatg gcgcaaatgg aaaggatggt
    14581 attacttcac agtcaacagg attcacacaa tgctgctttt ggctttaacc aggatgttcc
    14641 atattttgca catatccgtt tgcgtgaaaa caaacgagaa cgggatttgc tgataggaaa
    14701 tcagaattgc ttttccacac acttaccctg tccaattgtt gattggaaaa acgccccaat
    14761 tagtcagatt ttttaccgct atcatgaagg cgatgaatac gttgaagata ttggtgaaag
    14821 cgaattggaa ggtgagttga taacgagaag aatgctgctg attgagaacg gcattcttat
    14881 gcgaataaac tggcctggag gtgtacttga agacttatct acagagccaa ctaaacataa
    14941 taaatggcac ctggtcagtc ataaaacacc tcagcttgaa accggccagg acaaaaccct
    15001 ggatgaacta caaccacctg ataaatatac aagcagcaat ggaaaaacaa cagacctcaa
    15061 tttttccggt tacagggtgg acaagcatct gcggcaaatt actgcgctgg tggatcctga
    15121 gcaattcgaa gtaataacac attctgaata cgggattgtt ctgatacagg gaggtgccgg
    15181 ttctggaaaa accaccgttg cccttcatag actggcatat ttgatgtcaa aaaagcctca
    15241 atactttaaa ccaaaaactg tcctgccgat tgttttcgga ccggctttgg caaactatat
    15301 gtccagggtc ttgcctgcat tgggcgttca tggcgcaaga ccacgtactt atcataaatg
    15361 gttgtccgga ctgcgcatcc gcatgctgcc cgaattaccg gttcaatatg ctgaaaacac
    15421 tccgatggat gtgatcgaac ttaagcgtca tccgttgtgg ttgaaatact accgtgaaga
    15481 aatacaaaac cggacaacaa agttccgcga actgtttgag gataaactag ccaaagtcga
    15541 gggattggaa attttgctca aggcttggga tgcaatgtct gatacagctc tgattccacg
    15601 cctgcagaat ttggcaggtt ggtcaaaagg tgaagaaaca attcaaggtg taccacagct
    15661 ttccagtgcc agaatggaaa aacaactcca aaatgcatta gaacaggcat tccctgagct
    15721 aatggaatcc ccacatttat taactactca aatttggaat gattgtctag tcaaccggga
    15781 attgttattt gagacagttg agaggcttgc acctggtgaa tttaatgaat ctcaactcac
    15841 cggcgtttgg tcttgggcag tcagacaata tcaaaagcgt caggaatcaa aagaaattgt
    15901 agagtcagat ctggtttcgt tgaccgaaca aacggaagac ttctttaacg aagatttgaa
    15961 tttcacagac gaaaaatcct cgcttgatga agaagatgat actctcttgc ttttactata
    16021 tcaactgctg ttgggacctt tgaatcgtaa aaacggcaag cctctccaat atacgcattt
    16081 gatgctcgat gaggctcagg attttggttc acttgagttt cagctattag tcagtttaac
    16141 tccggaaaat cttcccagtg ttacattggc aggagatttg gatcagcgca ttatggttgg
    16201 acgtaaacat gaaacctggg aagagtcact gggccatttg acattacccg atggctcaaa
    16261 accagatgtg actgccctgg ctcctttaaa aattggttac cgttctactc atgaaataat
    16321 gacagttgca aaaaaagtta ttggcgaaca tagcgtaaat acagtttggt actcaacacg
    16381 acatggtgct ccggttgatg ttttccgttt caaggaacgt ggtgcaatgg tggcatttct
    16441 tgcagacaca ttgaaagacc tgtctattcg cgaaccactg gccagtgtgg ccgtgctgac
    16501 acggactcag gaatctgcag agaagctgtt tgatggtttg cagcgcacag atctttctca
    16561 attgcgttta atcagagatc aagagtttac attcacacct ggtattgaag ttacagatat
    16621 cacccagaca aagggcctgg aattcgactc agtcatcatc gcggatgcag atgcttcaac
    16681 atatggaccg gacatacgat ctcgacaatt gttgtatgtg ggagttacac gcgcggcaca
    16741 tcaactttgg cttcttcact gtgggaatcc gtcatcgttg attaccggga tcttggataa
    16801 gtagaatccg gattaaaatc aatttatggt tcgacgaata ctttttgatc tcctggattc
    16861 acactgatct atactgactc acttaaccaa aattattaat gctctaatgc agccagttat
    16921 taacaatcaa atcggcagtt taaaagaatc tgccacttta ttcattaacc agcttgcctt
    16981 aaagttacgc agtgaagggc aaaacatctg tcacttgggt tttggcgaat caccatttcc
    17041 ggtacccgaa cctatgcgaa ttgctttaca ggaaaacgct taccgcaaac aatatatttc
    17101 cggatatgga ttaccggaat taaggaaggc agttgcagga ttttttaaaa gtgaatttgg
    17161 ctataactac tcatttgaaa aaatattcat tggcccggga agtaaggaac tgatattcca
    17221 actagtttat ttattggagg gccccctcat ggttcccgct ccgagttggg tcagttatgg
    17281 gccgcaggca aagattcgct cgaaaacatt cattccaatt ccaactgacc tgaaaaacgg
    17341 atatcggtta caggcagaag aattggaaaa aacatgttcc ggtctgagtt ttccgcaaaa
    17401 attattaatt cttaataatc ctagcaatcc aacaggaagt gtacatactc cggaggaatt
    17461 aaaggatttg gctgaagtat gccggaagca tggaataatt gtaatttcgg atgaaattta
    17521 tgcgctgact aattttggtg aacaaccctt tgagggaatt gccaaatatt atcctgaagg
    17581 aacaattaca acaagtggtg tttcaaaagc cttttctgcc ggaggttggc gtcttggttt
    17641 tgcgatgatt ccggatgaat tagctaaaat agtaccgcca ttatctgcat ttgtgagtga
    17701 aactttcagt tgcgttagtg ccccagtaca gtacggagca ctttcgggat ttgaaaatta
    17761 tgattcggtg aggaaacatg ttgagcgctg ccgtgatatt cattgtgcag caggtcattt
    17821 tttacatcag cgattcacgg aaattggctt gaattgtcca aaacctcaag gagcttttta
    17881 tctctttcct gactttgaaa attttaaaga aaaacttagt tcccacggaa tcactacgag
    17941 tgaaaaattg agcagacgtc tattggaaga gacaggtgtt gcattgcttc cgggatcaga
    18001 tttttatatg ccgaatgact ttttagtagt ccgtgtagca agtgtagatt atgatggagc
    18061 gcagttgctc aagaattttc cagaggaaaa tcagctcact ccggaaatgt ttccaaacct
    18121 gtttccaaaa ttggcggatg cagctgagcg tattgaaaag tggcttagtt gaacaatttc
    18181 cagcaaaata tgttaaattt aattcataaa atgtttacct tgatcttaaa tcagcaaggg
    18241 ctggtaaact ctgttttcag gctcacccga aatgctttgc tgtataaaaa tacaggcacc
    18301 attcatgcgg atataaagct tggaaagtaa accggtttaa gagggaaaac aacggtcaca
    18361 ttgcaatcac gcaaagacgc aggtaataag atattgaaaa aaaggtttaa gaatgaagtc
    18421 agttaaaggc tatttgggat ctgatgtacg aatggatgga agtctcgaaa ccaatgatag
    18481 tcttcgtatt gatgccacct ttgttggaaa tgtttcaagc agtcattctg tcgtagttgg
    18541 aactcgagga agagtgatgg ggaaaataca agcacctgtg atcaaggtcg atggttgggt
    18601 tgagggtgat ctaaaagctg gtaagctggt agaagtttta ggtaatgcac gtattaaagg
    18661 aaatatttat actccagtcg gtggtttgaa aatgaggtta ggcggagaat tcaagggcaa
    18721 atttatcatg cattttacta aatagtaatg gagcttcaaa caggcagttg caatatttta
    18781 ctttaagcaa ctggattcgg tccaggtgaa ttccgtgcag
//
LOCUS       DTVD01000225           18572 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_530_length_18572_cov_424.428957, whole genome
            shotgun sequence.
ACCESSION   DTVD01000225 DTVD01000000
VERSION     DTVD01000225.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 18572)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18572)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18572
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_530_length_18572_cov_424.428957"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(44..1201)
                     /locus_tag="EYO68_07925"
     CDS             complement(44..1201)
                     /locus_tag="EYO68_07925"
                     /inference="COORDINATES: protein motif:HMM:PF01136.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07770.1"
                     /translation="MQFSTYLESLSQLKQILKTSIREVILEHKSLSRMGSLDTKSLLP
                     LIDAALVAEMSPVLQWDILSTEKTFQHSLSLLSRLPLAKFYAIRVQDLGAAEWIRQEH
                     PELPLHLIVETGNHNLTGLQRWIKYFGPQLQRLVLSTELPKSILKEYSQILTVPCEIL
                     AAGRILLFYTPRKLLNLHSLSHDTSYCIEKTLIPSDQPQRMFPTIENQHGTFMFHHRD
                     LFLLDFLPELENTGLSVLRLDLRHLNFSGTWIKKIDGLLESFDKKKIKVLKSKWPTKI
                     THGFFRANRTDLSIERIKNPHLKDHGETLTGYVVEAVKEEHLILLTRKSFRRGTPLLG
                     ITPEGRECEILTDSIHTTDGQPAEDIISGKLYQVPHVKYVTAQTLVYSRSE"
     gene            complement(1201..1974)
                     /gene="ccmA"
                     /locus_tag="EYO68_07930"
     CDS             complement(1201..1974)
                     /gene="ccmA"
                     /locus_tag="EYO68_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIC07771.1"
                     /translation="MRKRNLFLMKLIQSRDSIKSFISFSSMSALLSLERVSKRFGFRS
                     ILENICFSLDAGEFVMLIGNNGAGKTTLLHIVSSLMKPTRGTISFRGRNRKEHLQEWL
                     HIIGSLSHENRLYGDLSSIDNLRLFGTLYGVNELNIKIDNILEQIDLTHVARLPVRNF
                     SSGMTKRLMIGRLMLYQPKILILDEPYTGLDQHSFHWFQDYLQKFHQQGGTVLMVTHQ
                     LELGLELATRVLVLHQQGIQHDISTTGLSVDQCSAWLEE"
     gene            complement(1925..2863)
                     /locus_tag="EYO68_07935"
     CDS             complement(1925..2863)
                     /locus_tag="EYO68_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07772.1"
                     /translation="MEHNFFVVGENRVLAFLLNNIKYILWFLIIIIAALSGVIAFLHY
                     TGIQNQEQFKLQIDQEKTRSNSLISKLKKSSQQDSEWEQQILSQSSKQKKLKEQQESL
                     IDRQGKELSEIKSILDQREQVVQQFQLEKTSTQIQINNLQLKVRSLQQQLLSAQASLT
                     ESKTETENVQTKISELETVISKLEQQNKKLKQKASSPVTAVKKRKPLIIDDPTITVEK
                     LKITAKGTRVSVSYNLTNKSKKLQRGRTGMHLSSEKNLENEIPFRRQTSIPYKIKRYR
                     VISRKFTKVKPGTFIRILIWNEKKELIFDEAHPIKR"
     gene            complement(2881..4062)
                     /gene="dprA"
                     /locus_tag="EYO68_07940"
     CDS             complement(2881..4062)
                     /gene="dprA"
                     /locus_tag="EYO68_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HIC07773.1"
                     /translation="MTENVLKPDKSDPAWSWLALSLISGIGYKKIRDLIEHLGSVEDL
                     LKTSPEILIEQFQISSKLADLISKATQSHSFNIEKRIIEESPGVRLFCPDSSGYPLRL
                     QQISTPPSVLYWQGELQDAESPCLAFVGSRGCTAYGKQQTRRLVKEISQYVPDMIIVS
                     GLAKGIDTVAHETALEFGLKTISVLAGGLQHIYPPENKKLAEEILKNGALVSEFPLGV
                     KPLARNFPIRNRVISGLSMGIVVTEARKKSGAKITAAFALEQNREVFAVPGRVDSAAS
                     SGTNSLIARQHAKLVSSAEDILEELSLISATATNLPLSPDGETGFKKINPDDLGEKQS
                     RILKALNDGVEEIDAIYEQTGLEMNFLLATLLELELTGTVENIGGQIYRLRVELEIPS
                     N"
     gene            complement(4074..4529)
                     /gene="smpB"
                     /locus_tag="EYO68_07945"
     CDS             complement(4074..4529)
                     /gene="smpB"
                     /locus_tag="EYO68_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIC07774.1"
                     /translation="MASKTLAKNRKARFEYEIQKTMEVGIVLLGTEVKSVRAGQINLA
                     ESYCRVDDSLQVYLLNAHISQYDFGNRHNHEPLRPRRLLLHRSEIRRLYGQVKEQGLT
                     LIPLKIYLKGGIIKMEMALGRGKKLHDKRQTMKKRDAQRDVERALSERT"
     gene            complement(4592..5986)
                     /locus_tag="EYO68_07950"
     CDS             complement(4592..5986)
                     /locus_tag="EYO68_07950"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01743.18,HMM:PF13735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIC07775.1"
                     /translation="MPLLSTPPELIKIANAIAKSEGRSILVGGAVRDHCMGRKSLKDF
                     DVEIFGISPEVLESVLRNFGNIHAVGKSFGVLKLKTSSAEYDFSLPRRESRTGKGHRG
                     FMVTPDSTMSYREAASRRDFTVNSIGYDLLTKTLLDPFDGLGDIKHKILRHVGPAFAE
                     DPLRVLRAMQFSARLEFKIAPETVQLCKTLDLAELSKERIFEEFRKLLLKAKRPSIGL
                     EAAKVLGILAYFPELKALIGVPQDPKWHPEGDVWTHTLLVLDEAADLRKGNEKLDLEL
                     MFGALCHDFGKPLTTEFLQGRWRSPAHDVEGVAPTEQFLRRLTDDRVLIEQVTILVKE
                     HLRPIQLFKERERINSGTIRRLALRVRIPELVLLAKADYLGRTLTDEERKSFDAGDWL
                     LAEAERMNVRDDSPRPLLMGRHLLAMGMSPGPEMGEVLNEAFEKQLNGDLQTEDDAIT
                     WVKSNLPNLSNLAP"
     gene            complement(6168..7106)
                     /locus_tag="EYO68_07955"
     CDS             complement(6168..7106)
                     /locus_tag="EYO68_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC07776.1"
                     /translation="MKKVLVTGGAGFVGSFLCDRLIDEGHEVIAIDNFFTGSKENLSQ
                     LSDETNFELIRHDIVKPILLEVDWIFNLACPASPIHYQYNPVKTVKTNVMGTLNMLGL
                     AKRVKARILQASTSEVYGDPQVHPQPESYFGNVNPIGLRSCYDEGKRVAETLMMDYHR
                     QSQVDVKIVRIFNTYGPRMHPEDGRVVSNFIVAALKGEPITIHGDGLQSRSFCYVTEL
                     VDAMYRMMNTENFTGPVNTGNPGEFTMIELAETVLKMTNSSSKLVKVEARPDDPGRRQ
                     PDITLAKEKLGWEPEIKLKEGLKPTIEYFDELLKRS"
     gene            7496..8533
                     /gene="recA"
                     /locus_tag="EYO68_07960"
     CDS             7496..8533
                     /gene="recA"
                     /locus_tag="EYO68_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIC07777.1"
                     /translation="MLTEERQKDLETAIQQIERQYGKGSIMKMDGSTVGHYPAVSAGN
                     LALDIALGIGGLPRGRIVEIYGPEASGKTTLALCAIAEIQKIGGIAVFIDAEHAFDRN
                     NAKTLGVQVEDLLLAQPDYGEQGLEIAEQLVRVGKVDMVVIDSVAALVPKNELEGDMG
                     DSHMGLHARLMSQALRKLSGVISKSNTIFIFINQLRSKIGVVFGNPETTTGGNALKFY
                     STVRLDVRRASQIKSGDEVKGHRMKIKVAKNKLAPPFRIAEVDLIYGVGISKMGVLID
                     LAVNDDIVQKSGTWFSFEKDRLGQGRENSIRFLEENPEIADTINLKVRKIHNLDFSDT
                     SEETQETAEDE"
     gene            8530..9618
                     /gene="mnmH"
                     /locus_tag="EYO68_07965"
     CDS             8530..9618
                     /gene="mnmH"
                     /locus_tag="EYO68_07965"
                     /inference="COORDINATES: protein motif:HMM:PRK11784.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIC07778.1"
                     /translation="MSTCDSLPDQSKHPQKSAEDFLNGSIGLFSAEEALSDGIILVDV
                     RTAEEFAKGSIPEALSRPLFDNMERAEIGTIYKQIGRDAAVDKGLKIIEPRLQQFLKS
                     LLDLKSHQLVIYCARGGMRSASVVRLLQHHGFRAAQMQGGYKRFRQYVLQQLESPMPP
                     FIVLHGQTGVGKTLLLNKLPDHIDLEGLAGHRSSLFGAINKTPRSQKNFEGLLVQKLR
                     TLPEVLPLFVEGESRKVGQVFLPYSLANAMKSGMLVLLSASMNTRISRIVEEYQICDE
                     QSFRQVDSILITLRVSLGKLKVDQLRLWLKKEEIEKIVHMLLVDYYDPLYMHSMSSYQ
                     YVLELSAEDLNLAAVELIHFRDEVIKSH"
     gene            9680..12355
                     /locus_tag="EYO68_07970"
     CDS             9680..12355
                     /locus_tag="EYO68_07970"
                     /inference="COORDINATES: protein
                     motif:HMM:PF04733.12,HMM:PF13181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07779.1"
                     /translation="MRRSNSLFYLIFTIILVFQVASGFAESRENVATVKTHSGSFPLP
                     IPTLRLPDHPGSPLVTVPDAVFPELPDWHYSRFIPEDPSWLDSAGKFYKEGLVYLFRG
                     DLNVAFKRFQSVTDNYPETPWFAPSLFWQAQILSKRKEYVPAGKILTVFLDSLKQGKS
                     SERYIDFQNFSRYTLAWLALKQKKYNEALEIIEKNKEVISVKKIRIQLLFLKYLIHVQ
                     LKQSDLIFEVIDDLIQLFPYDFEHVVRLAEFYFVENRWQELADLVEAQAREQVFYNDP
                     RMEHFLWLGVAAEMRLKHWNKAKKRLQSLEKFGVRSPDKIALAYLRLNLYTNQIESSW
                     RNWFKIQDDIVREKALRELIHHAVKTEEFLFLIKKQPELKSVTQYWREWQGELELIYA
                     YLYLRQGQIKKAKQFLLWAQNNLKQESNQVPLIVKEESLYLGTVIELLLKDHQKALQH
                     LKQLLAEYAGSERQSDYYFWYGVLLYELEKSYLQTIMAMRQVDREGERDDDRLFLLGK
                     VNHDQQKWRAVISAFAKLQKLHPGSQFLEEGLYLQAQAFFELKQYNSGLEILNELQNT
                     FGQLKKPVRAVHLRVQILVAMQRYEQADDVLQRMIEENSDFSLIKLRVEVLKYIKDPR
                     RILSVTGVGLELSTNKDQGFLYFHRANALFDTKKYEEANTYYTLALKNPPKDTRRVIN
                     YRILKIQYELGRIPELLQGADLFLKVSRDDFYSYEILHMLSNYFLEKKQREKAFSYLK
                     QLVANYKKSVRKVELAPEKRLEQIVLIGQLYNELTNYELAERWLNQALKSMETVEEGR
                     KKWQLHIFKEKGFALYKLGKHRQALAASLKVLYLDRSLSNQQRYDLNLRIASSYVQLK
                     RTKEARSIYLKMQKKFKDTVRQKEIEKLLRSLMNQ"
     gene            12526..13209
                     /locus_tag="EYO68_07975"
     CDS             12526..13209
                     /locus_tag="EYO68_07975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIC07780.1"
                     /translation="MDFKIIAIDGPTGVGKSTIARQLAEKLGCLYVDTGAMFRCLALC
                     WGKHGCPESDDSLQELGDQTRIVFKHKNVICNGKDVTDEIRTELISSLASKISSFSSI
                     REVMKKQQRTLVEEVRKSESYLGAVLEGRDIGTVVFPDAEFKFYVDASPEIRAKRRML
                     QLQERGENANFEKILTALRKRDFQDQNRKVAPLRPAEDALIVDTGNLSADQVLEHLVA
                     CIGEEKNVS"
     gene            13262..14452
                     /locus_tag="EYO68_07980"
     CDS             13262..14452
                     /locus_tag="EYO68_07980"
                     /inference="COORDINATES: protein motif:HMM:PF12804.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU protein"
                     /protein_id="HIC07781.1"
                     /translation="MKAVILAANRSNRLSPFIETRCKPMIRIAGQYILENTICFLKEA
                     GINDIVLVVNHQQDLVRRYFGSGKTLGVNLEYVVQDELQGIGQALSLCQSSLEKKPFL
                     LVYGDVMTDYNPFLSILQTYSEMDKEVALIALPESSKEFGNVYLDHEMKISRLVEKPK
                     VNQANYVFAGMFVLFPKFFSLLEEHQNDISQCYGQMIETSGLQACLWEKGWIDMIHPW
                     HILEANRMLMSSWETAEIDSTARLLGQVHLEGSVRIGPNVTVESGTILKGPCYIGSNS
                     YVGNNSLIRNYSAIGPDSIVGYGSELKNCVLFGNNDVGRLSFVGDSVLGENVKSGSGL
                     TTVNHTVDYSRITCKSNSDSIETGLTKLGAFIGDNVSIGARHTLGPGTIVESGKIVAD
                     NITL"
     gene            14491..16308
                     /gene="glmS"
                     /locus_tag="EYO68_07985"
     CDS             14491..16308
                     /gene="glmS"
                     /locus_tag="EYO68_07985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIC07782.1"
                     /translation="MCGISGIVHSENIGRNLFNSIRNLEYRGYDSCGMALLRQGALDL
                     RKNVGGIDEVNSLEKLDQMQGSIGIAHTRWATHGGVTQENAHPHLSQNKQFVIVHNGI
                     ISNYQELRERMLSQGVVFESLTDTEVFVNLLEESYSNSKDIEQAFLAALEQIEGSFSI
                     VMLSAHDPEHLYCVKKESPLLLGLGEGCNYVGSDLNAFLEYTRKAVVLDDGEYVVLGR
                     NDYKIRKLKTREHVEKNVLHIDWDVETSKKGGYSHYMLKEIFDEPQTIRQALKIPHEQ
                     ISGLALMFTEATRAYLMGVGTTYYVANISQYFFSTLANRYYPALSSDEFSVVSVESGD
                     MVLALSQSGETYDTKSSLEYAKLHGAQTAAVVNVMGSAISMMVDQVIMQGSGPEICVV
                     STKAALAQTFIMLRVALELGRMRKHISQDVYQKHQRELEIFPQMVDQILNEQSGFVRN
                     IARTTSHVEHWLFLGCGVYYPVAMESALKMKEITYQHAEGMPAGFLKHGTLSMVEPAL
                     HSLFFVPLPEQKDLHDRTLIAMEQVKARGGTVIGIMFQGDKHAQSVTDQAVLLPQMSA
                     LCAPMAQMIMAQMFAYYAALDLGRNIDKPRNLAKSVTVG"
     gene            16399..18516
                     /locus_tag="EYO68_07990"
     CDS             16399..18516
                     /locus_tag="EYO68_07990"
                     /inference="COORDINATES: protein motif:HMM:PF02878.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07783.1"
                     /translation="MTIERIQGTDGIRGIVRLAEDCSGSDPISVFLHEAVLTEEFFEL
                     YTYAYCQELLEADFASANDLAVIGWDPRDMSGSFNHSAIRGIRKAGLTAVVVDILPTP
                     AISLYQLHVGAACAFVLTASHNPADQNGIKIFIGHSNLKLFPEDDKRLTQRCLSFNFE
                     NLRSAPLIGELRNDHAAARKLFIEFMADPQNHWLNKDSLAGAKIIVDAANGAFSPLIE
                     DLLNYESADYIFTNCDPARGINVRSGVADLEGVSSISADEIENGLFSGYETLEKMLAS
                     GREQKEKLLNNSGQVLGFVFDGDGDRCFLLSYDPFQDCVLVVGGDAQAFFQAKLLQQN
                     YSWNQGPLFVNTVESDLEAGRAVQQIGFETMQCAVGDKWILWQACFYDWQAKREFYLN
                     KIAASEFRILQEQVRTRLEQMVQSTKFDALSATTTFMSLEKWVSNNIGNELVNSAYDH
                     VCQQQNNIFAIGSEESGHVITLAKMNFGHVNRPVFIGNGLKCALNSLAAIQALRSAKN
                     TPEFFEWLKCPFPSGFQKSLPVYYVDKSLLDEGSVLRTELEELLLANLNWPEMEVEIV
                     KRQEEPEMLLLNVLENGLPAAAVFVRNSGTEDKLALYLRGRKELEVRLESLAEKIYPF
                     LLLSFKNKNSPMAQAEQLVLLRLKDGAKQAIDLSFEQFAKISLNRLLHEMSSRQQLIK
                     QEGDTWSITETGRICLNHTERSE"
BASE COUNT         5346 a         3544 c         4125 g         5557 t
ORIGIN      
        1 gttctaaaac cttaagtgtt tggtaaaaat atacaagccg taattactca gaacgactgt
       61 aaaccagggt ctgtgcggta acatatttta catggggtac ttgatacaat ttcccggaaa
      121 ttatatcttc tgcaggctga ccgtcggtcg tatgtatgct gtccgtcaat atttcacatt
      181 cacgtccttc cggagtaatt ccgagcaaag gagttccacg ccggaatgat ttacgggtca
      241 gcaatatcag atgctcttct tttacagcct caactacata tccggtcaat gtttcaccat
      301 gatccttgag gtgcggattt tttatgcgct caattgacag gtcagtcctg tttgcccgaa
      361 agaagccatg tgtaattttg gtgggccatt ttgattttaa taccttgatt ttctttttat
      421 caaatgattc aagcagtcca tcgattttct taatccacgt gcctgaaaaa ttcagatgcc
      481 gcaaatccag ccttaaaaca ctcaagcctg tattctccag ttccggaaga aaatctaaga
      541 gaaataaatc tcgatgatga aacatgaaag taccgtgttg attttcgatg gtcggaaaca
      601 ttctttgggg ttgatcactg gggatcaaag ttttttctat gcaatatgag gtgtcatgtg
      661 aaagactatg taaattcaat agcttacgag gtgtgtaaaa caatagtatt ctgccggcag
      721 ctaatatttc acagggaaca gtcaagattt gactatactc cttcaagatg gattttggca
      781 actcagtgga aagaaccaaa cgttgcagct gcgggccaaa atatttgatc cagcgttgaa
      841 gccctgttaa attatggtta ccggtttcaa ctatcaaatg aagtggaagt tccggatgtt
      901 cctgccggat ccattctgca gctccaagat cctggacgcg gattgcgtaa aactttgcta
      961 aaggcagacg actcaacagc gaaagactgt gctggaatgt tttctctgtg ctgagtatgt
     1021 cccactgtaa tacaggtgac atttcggcaa caagagctgc atcgataagt ggaagcaggg
     1081 actttgtgtc taagcttccc attcgactca gtgatttgtg ctctaaaatt acttcacgga
     1141 ttgaagtttt taggatttgt tttaactgcg agagactttc gaggtaagta ctaaactgca
     1201 ttattcttcc agccaggcac tacactggtc gacacttaat cctgttgtgg aaatatcatg
     1261 ttggattcct tgctgatgta acacaagtac acgggtcgct aattccagcc ccagctcaag
     1321 ttggtgtgta accatcaata cggtaccacc ctgttgatga aatttctgaa gataatcctg
     1381 aaaccaatga aatgagtgtt gatcaagtcc agtgtaaggt tcatcgagga ttaaaatttt
     1441 cggttggtac aacattaaac gacctatcat taaacgcttt gtcattccgc tgctaaaatt
     1501 acggactggc agccgggcaa catgggtcag gtcgatttgc tctaaaatat tgtcaatttt
     1561 tatgttcagt tcgttcactc cataaagagt tccgaacaga cgcaaattat ctattgaact
     1621 gagatcacca tacaacctgt tttcatgcga caggcttcca attatatgaa gccattcctg
     1681 caagtgttct tttcgatttc taccacgaaa agaaattgtt ccacgggtgg gcttcatcag
     1741 tgaactgaca atatgaagca aagtcgtttt gcctgcaccg ttatttccaa tcagcatcac
     1801 gaattcgcct gcatccaaag agaaacaaat gttttcaagg atggaacgaa aaccgaaacg
     1861 tttactgact cgttcaaggc ttaagagagc agacatgctg gaaaaagata taaaggattt
     1921 tatgctatct cttgattgga tgagcttcat caaaaataag ttcctttttc tcattccaaa
     1981 tcaggattcg aatgaaggtt ccaggcttaa ctttggtgaa tttccttgat ataactctat
     2041 atctcttgat tttatatgga atggaagttt gcctgcgaaa tgggatttcg ttttcgagat
     2101 ttttctcaga ggaaagatgc attccagtgc ggcccctttg gagctttttt gatttattag
     2161 taaggttata actgacactg actcgagtgc cttttgcagt tatctttaat ttttctacag
     2221 ttatggttgg atcatctatg atcaggggtt ttcgtttttt taccgccgta acaggagaag
     2281 aagctttttg ttttaatttt ttattttgtt gttcaagttt gcttattacg gtttccaact
     2341 ccgaaatttt tgtctgaaca ttttcagttt cagtttttga ctcagttaat gatgcctgtg
     2401 cacttagaag ttgctgttgt aaggaacgaa ccttaagctg aaggttgttg atctgaattt
     2461 gagtagatgt tttttcaagt tgaaactgct gaaccacttg ttcacgctga tcaagaattg
     2521 atttaatttc agaaagttct ttaccttgtc gatctatgag ggactcttgc tgttctttca
     2581 gcttcttctg ctttgaagac tgggataaaa tctgttgttc ccattcactg tcctgttgac
     2641 tagatttctt taattttgag attagactgt tacttcgagt cttttcttga tcgatttgca
     2701 acttaaactg ttcttgattc tgaatgccag tataatgtag aaatgcgatt acaccggaca
     2761 atgctgcaat aattataatc aaaaaccata aaatatattt aatattgttc aggaggaatg
     2821 ccaaaacgcg gttttctcca acaacaaaga agttatgttc cattttgcta atcgaatgta
     2881 ttaatttgaa ggaatttcaa gttcgactct gagacggtaa atttgtccgc caatgttttc
     2941 tactgtacca gttaattcta attcaagcaa ggtagccaac aaaaaattca tttcaagacc
     3001 tgtttgttcg taaattgcgt caatttcctc aactccgtca ttcaatgctt taagaatcct
     3061 actttgtttt tcacccaaat catcaggatt tatctttttg aatccggtct cgccatcagg
     3121 agaaagagga agattggttg cagtggcaga aataagtgat aattcctcca aaatatcttc
     3181 tgcactgctg accagcttag catgctgacg cgcaatcaaa ctgttagtac ctgagcttgc
     3241 agcggaatct acccggccgg ggacagcaaa tacttccctg ttctgctcaa gtgcaaatgc
     3301 ggccgtgatt tttgcgccgc tttttttgcg ggcttcagtg actactattc ccatggacaa
     3361 accactgatt acacggttcc ggataggaaa atttctggca agtggcttta cgcccagtgg
     3421 aaattcggaa accagtgctc cattctttaa tatttcttca gccagctttt tattttctgg
     3481 aggataaata tgctgcaacc cacctgcaag tacggaaatg gtttttaatc caaattccaa
     3541 tgcagtttcg tgtgccactg tgtcgatacc tttcgcaagc ccgcttacaa taatcatgtc
     3601 cggaacatat tgtgagattt ctttaacgag tcggcgcgtt tgttgttttc catatgctgt
     3661 gcaacccctg gagccgacga aagcaagaca tggactttct gcatcctgta attcaccctg
     3721 ccagtacaat acacttggtg gggtggatat ttgctgtagc cgcaaaggat atccggatga
     3781 atcaggacaa aaaagccgta ctccgggcga ttcttcaatg atccgctttt ctatgttaaa
     3841 actatgagat tgcgtagctt ttgaaatcag gtccgctaat tttgatgaga tttgaaactg
     3901 ctcaatcaag atttcagggc tcgttttcag cagatcctct acactgccta agtgctcaat
     3961 taaatcccgg atctttttgt agccgattcc ggaaatcagg ctcaatgcca gccaagacca
     4021 tgcaggatca gatttgtcag gtttaagaac attttccgtc atagcgctcc attttatgta
     4081 cgttcgctga gtgcacgctc aacgtctcgc tgagcatctc gctttttcat ggtttgacgt
     4141 ttatcatgca gcttttttcc acgtccaagc gccatttcca tttttataat tcctcctttg
     4201 aggtatattt tcagaggtat caatgtgaga ccttgctctt ttacctgccc ataaaggcgg
     4261 cgaatttctg agcggtgaag tagtaagcgt cttggacgaa gcggctcatg attgtggcgg
     4321 ttgccaaaat catattgaga aatatgtgca ttcagtaaat atacctgcag gctgtcatca
     4381 acacggcagt aactttctgc cagattgatt tgaccggcac gaacactctt cacctctgta
     4441 cccagcaaga caatgccgac ttccatagtt ttttgtattt catactcgaa ccttgcctta
     4501 cgattttttg ccagagtttt ggaagccatg tctgtgaaag aatttctata aaattacctt
     4561 ggaatgaatg tgaattataa cggtcttacc ttcaaggagc aagattggac aaattcggca
     4621 aatttgattt tacccaggta attgcatcat cttctgtctg caagtctccg tttagctgtt
     4681 tttcaaatgc ctcattcaaa acttctccca tttctggtcc gggtgacatt cccattgcca
     4741 agaggtgtct ccccattaac aaaggacgcg gtgaatcatc acgaacattc atccgttctg
     4801 cttcagcaag cagccagtcg ccagcatcaa aacttttacg ttcttcatca gttagagtac
     4861 gtcccaaata atctgccttt gccaacaaaa ccaattcagg tatcctgaca cgtaaagcca
     4921 gtctcctgat tgtaccggaa ttaatccgct cgcgctcttt aaaaagctgt attggacgga
     4981 ggtgttcttt gaccaaaatt gtaacttgtt caattaaaac tcgatcatct gtcaatctac
     5041 gcaaaaactg ttctgttggg gcaactccct cgacgtcgtg agcaggactt cgccagcgtc
     5101 cctgcaaaaa ttcagtggtc agcggcttgc caaaatcgtg acaaagcgcc ccaaacatca
     5161 gctctaagtc cagtttttca ttaccttttc ttaagtctgc tgcctcgtcc aaaacaagca
     5221 gagtgtgtgt ccatacatct ccttcaggat gccattttgg atcttgcggg actccaatta
     5281 gtgctttcag ttctgggaaa taagctaaaa tgcctagaac tttggcagcc tctagaccaa
     5341 ttgaaggtcg ttttgctttt agcagcagtt tacggaattc ttcaaaaatc cgttccttgg
     5401 ataactctgc aaggtccagt gttttgcata attgcacagt ttcaggcgca attttaaatt
     5461 ccagacgtgc cgaaaactgc attgctctca atacccgcaa aggatcctcc gcaaatgcag
     5521 gtccaacatg ccttaatatt ttgtgcttta tgtctcctaa accatcaaat ggatcgagca
     5581 atgttttggt taacagatca taaccgatac tgttgactgt aaagtcacgg cgagaagctg
     5641 cttcacgata gctcattgta gaatcaggcg ttaccataaa tccgcgatgt ccttttccag
     5701 tcctgctctc acgtctgggc agactaaaat catattctgc acttgaggtt ttaagtttca
     5761 gcacaccaaa actttttcca acagcatgaa tatttccaaa attccggagt acggattcca
     5821 gtacttccgg actaattccg aatatttcca catcaaagtc ttttaaactt ttgcggccca
     5881 tacaatggtc cctcaccgca ccaccgacta gaatactgcg tccttcggat ttggcaatgg
     5941 catttgcaat ttttattagt tcaggaggag ttgacaggag gggcattaag tctttcaggt
     6001 ttaataaatc atttcgttgt caaacatatt ttgtttcaaa caagtcaata ataatatttt
     6061 tccggaatgg tttggcttag cttagcagtt gaagaattga ttaagtccta aagacttttc
     6121 taagcaattt atgttaatag tgtttaagtt gaaccggata cttattatta gctgcgtttc
     6181 aatagttcat caaaatattc tatggttggc ttcaggcctt ctttgagttt tatttcaggt
     6241 tcccagccaa gtttttcttt agccagcgtg atgtccggtt gacgtcgccc cggatcatcc
     6301 ggtctcgctt ccactttcac aagcttggag ctggaattag tcattttcaa tactgtttca
     6361 gccaactcaa tcatagtaaa ctcgccagga tttcctgtat tcaccggtcc agtaaaattt
     6421 tctgtgttca tcattcgata cattgcatct accaattctg tcacataaca aaaactgcga
     6481 ctttgcagtc catctccatg gatggtgatg ggttcacctt tgagtgctgc aactataaaa
     6541 ttgctgacga ctctgccgtc ttcgggatgc attctgggac cgtatgtgtt gaagatacgc
     6601 acgattttta catcaacctg actttgcctg tgatagtcca tcattaaagt ttctgccaca
     6661 cgctttcctt catcatagca gcttcgcaaa ccaatcggat tgacgttacc aaaatagctc
     6721 tccggttgtg gatgtacctg cgggtcacca taaacctctg aagttgaagc ctgcaaaata
     6781 cgggctttta ctcgttttgc caaacccagc atgttgagtg tacccatgac attagttttt
     6841 acagttttta caggattgta ctgataatga attggtgatg cagggcaggc caggttgaaa
     6901 atccaatcca cttctaaaag aataggtttc acaatatcgt gacggatcaa ctcaaaattt
     6961 gtctcatctg aaagctgact gagattttct ttgcttccag taaaaaaatt gtcaattgca
     7021 ataacctcgt gtccttcatc aatcaggcgg tcacaaagaa atgaccctac aaaaccggca
     7081 ccaccggtaa ctagaacttt tttcatttga ataatctctg tgaattgtga aaaagtacaa
     7141 ggaacattat atcctgatat aatagcaagg tacaaggaag tccggagtgt aaaggactaa
     7201 taatccaaaa tttctgcatg agtcagattt gttttaggta gaattcgttc aaccagagat
     7261 taagaaaact ttacatgcaa tctgaaatag cattgcgact tttttagagt cagattatta
     7321 tttgctgata gaatcctgct tataaacgta gaattaatta ctgaaaaaag cggtatcagg
     7381 atgtcgcttg aataacatgt tgtataatca agggttgaac gggaatttac gaaaatgtta
     7441 atcgagtaaa tccgtgaaaa ctttgctgag aaatttcatt attgaaggag gaattatgtt
     7501 gactgaagaa agacaaaaag atttagaaac cgcaattcag caaattgaac gccagtatgg
     7561 aaaaggttct atcatgaaaa tggacggctc gactgtgggg cattaccctg cagtgagtgc
     7621 cggtaatttg gcgttggaca ttgcactggg cataggaggt ttacctcgag gacggatagt
     7681 tgaaatttat ggtccggaag catctggtaa aacaacttta gccttgtgtg cgattgcgga
     7741 gattcaaaaa ataggtggaa tagctgtttt catagacgcc gaacatgcgt ttgaccgaaa
     7801 caatgccaaa actctgggtg ttcaggtgga agatctcctt ctggcccaac cagactatgg
     7861 cgagcagggt cttgaaattg cggagcagct tgtgcgcgtc ggcaaagttg atatggttgt
     7921 tatagattct gtcgctgcct tggtaccaaa aaatgagctg gaaggagata tgggtgattc
     7981 tcatatgggg ttgcacgcac gtttgatgtc acaggcactt cgcaaattat ccggtgtaat
     8041 atccaaatca aatactattt tcatttttat aaatcagctt cgttcaaaaa ttggagtggt
     8101 ttttggaaat ccggaaacaa cgactggtgg gaatgccctc aaattttatt ccactgtacg
     8161 cctggatgtc cggcgggcca gccagattaa gagcggagat gaagtaaagg ggcacaggat
     8221 gaaaatcaag gtggcaaaaa ataaattggc gccgccgttt cgtattgcag aagttgattt
     8281 gatctatgga gttggaattt ccaaaatggg agttttaatc gatttagctg tgaatgatga
     8341 tattgtgcaa aagagcggaa catggttctc tttcgagaaa gacagattgg gacagggacg
     8401 cgaaaactcg atcaggtttt tagaagaaaa tcctgaaatt gcagacacca tcaatctcaa
     8461 agtaaggaaa attcacaatc ttgatttttc agacacaagc gaagaaacgc aggaaactgc
     8521 tgaggatgaa tgagtacttg cgactctctg ccggatcaat cgaaacaccc acaaaaaagt
     8581 gcagaggact ttcttaatgg aagtattggg cttttttctg cggaagaagc tctttcagat
     8641 ggcattattc tggttgatgt acgtacggca gaggaatttg ccaaaggcag tattcctgaa
     8701 gctctgagcc gtccattgtt tgataacatg gagcgtgcag aaattggaac aatatacaaa
     8761 cagattggca gggatgcagc agttgacaaa ggtttaaaga ttattgaacc gagactgcag
     8821 caattcttaa agtcgctttt agatttaaaa tcccatcaac tagtcatata ttgtgcccgc
     8881 ggcggaatga gatctgcttc ggttgtcagg ctgttacagc atcacggatt ccgggctgca
     8941 caaatgcagg gtggatataa gaggtttcgg caatatgtgc tgcaacagct tgaaagccca
     9001 atgccgcctt tcattgtcct gcatggtcag acgggtgtgg gaaaaacctt gctcctgaat
     9061 aaactgccgg atcatataga tttggaaggt ctggcagggc atcgaagttc tttatttggt
     9121 gctataaata aaactcctcg gtcacagaaa aactttgaag gactgcttgt tcaaaaattg
     9181 agaacacttc cagaggttct acctctgttt gtggaaggag agagccgtaa agtggggcag
     9241 gtatttctcc catattccct cgcgaatgct atgaaatccg ggatgctggt gctgttgagt
     9301 gcatctatga ataccaggat cagccgtata gttgaggagt atcaaatttg tgatgaacag
     9361 agttttcgac aagttgattc aattttaatt acattacgtg tgtcacttgg aaagcttaaa
     9421 gtggatcaat tacgcctgtg gctcaaaaaa gaagaaattg aaaagatagt gcacatgctt
     9481 ttggtagatt attatgatcc tctctacatg cattcaatga gttcatacca atatgtacta
     9541 gagttgtctg cagaagactt gaatttggct gcggttgaat taattcattt tcgagatgaa
     9601 gtgatcaaaa gtcattaatt atttaaaatg tttttagcgg cacgatccat caaattaact
     9661 tttctaaaaa aatcctgaaa tgagaagatc aaattcttta ttttacctga tatttaccat
     9721 aatattggtt tttcaggttg cgagtggatt tgcagaaagt cgggaaaatg ttgctacagt
     9781 aaagacacat tccggctcat tcccactacc catcccaaca cttcgtttac cagatcatcc
     9841 gggatctccg ttggttacag ttcctgatgc agtctttccg gagcttcctg actggcatta
     9901 ctctcgtttt attccagaag atccaagctg gcttgacagt gcgggcaaat tttacaaaga
     9961 aggtcttgtc tatttgtttc gtggagactt gaatgttgct tttaagaggt ttcagtcagt
    10021 aacagataat tatccggaaa cgccatggtt cgctccgtct ttgttttggc aggctcaaat
    10081 tttatcgaag cggaaggaat atgttccagc aggaaaaata ctgactgtat tccttgactc
    10141 tctcaaacag ggtaaaagtt cagaacgata tattgatttt caaaatttca gccgctacac
    10201 attggcctgg ctggctctga agcaaaaaaa atacaatgaa gcactggaaa taattgagaa
    10261 aaataaagag gtgatcagtg ttaaaaaaat acgtattcag cttctttttt tgaagtacct
    10321 gatacatgtt cagttgaagc agagtgattt aatatttgaa gtaattgatg atttgatcca
    10381 attgtttcca tatgattttg aacatgtagt gcgtctggct gaattttatt ttgttgaaaa
    10441 ccgctggcag gaattagcgg atttagttga agcacaagct cgggagcagg tattttacaa
    10501 tgatcctcga atggaacact tcttgtggct gggcgtggct gcagaaatgc gtttgaaaca
    10561 ctggaataaa gccaaaaaac ggttacagtc tcttgagaag tttggtgtcc gcagtcctga
    10621 caaaattgct ttggcctatt taaggcttaa tctttataca aatcaaattg aaagttcctg
    10681 gaggaattgg tttaaaatac aggatgatat tgtccgggaa aaagcattgc gagaattaat
    10741 tcaccatgca gttaaaactg aagagtttct ctttttgatt aagaaacagc cggagctgaa
    10801 gtcggttact caatattggc gtgagtggca gggggaatta gaactaatat atgcttatct
    10861 ttatttgcgc caagggcaga taaaaaaagc aaagcaattt ttgctgtggg cacaaaataa
    10921 tttgaagcag gagtcaaatc aggtgccttt gattgttaag gaggaaagtc tttacctggg
    10981 tactgtgata gaactgctgc taaaagacca tcagaaagcg ttgcagcatt tgaaacaact
    11041 gctggcagag tatgcaggct cagaacgtca gtcggattac tatttttggt acggggtttt
    11101 attgtatgaa ctggaaaaga gttacttgca gacaatcatg gcaatgcgac aggttgaccg
    11161 ggaaggtgag cgggatgatg atcgcttgtt tttactcggc aaggtaaatc atgatcagca
    11221 gaaatggaga gcggtaatct cggcttttgc aaaattacag aagctgcatc caggttccca
    11281 attccttgaa gaagggcttt atctgcaggc ccaggctttt tttgaactaa agcagtacaa
    11341 ttcaggtctg gaaatattaa atgaactgca aaacactttt ggtcaattga aaaaacctgt
    11401 aagagcggtt catttacggg tgcagattct tgtggcaatg caaagatatg agcaagctga
    11461 tgatgttctt cagcgtatga ttgaggagaa ttcagacttt tcactgataa aattgagggt
    11521 tgaagtattg aagtacatca aagaccccag gcggatactg agtgtgacag gtgtcggact
    11581 ggagctttca accaacaaag atcagggctt cctttacttt catcgggcaa atgctctttt
    11641 tgatacgaaa aaatatgaag aagcaaacac atattacact ttggcattaa aaaatcctcc
    11701 aaaagacact agacgagtta tcaattaccg gatcttgaaa attcagtatg aattggggcg
    11761 cattccagaa ttactgcaag gtgcagatct gtttttgaaa gtgagtcggg acgattttta
    11821 cagttatgaa attctgcata tgctcagcaa ttattttctg gaaaagaaac aaagagaaaa
    11881 ggccttctct tatttaaagc agcttgtagc caactataaa aaatcagtac gtaaagtaga
    11941 acttgctcct gaaaagagat tggagcagat tgttctaatt ggtcaacttt ataacgaatt
    12001 gactaactat gagttggctg aacgctggct gaatcaggca ttaaaatcga tggaaacagt
    12061 tgaagaagga cgaaaaaaat ggcaactgca tattttcaaa gaaaaagggt ttgctctcta
    12121 taaattaggc aaacatcgtc aagctctggc tgccagtctg aaagttcttt atctggaccg
    12181 tagtttgtct aatcagcaaa gatacgatct caatttgcga attgcatcaa gttatgtgca
    12241 actaaaacgc actaaagaag cgaggtcgat ttacctgaaa atgcaaaaaa aatttaaaga
    12301 tacagtccgt caaaaagaaa ttgaaaaact cctgagaagc ttgatgaatc aataatgata
    12361 tgctccaatg gttccaacca ggatgtctga attgatctaa gccggagcca attttcattt
    12421 ttgctttatg gagtcagaat tttcatgagg aaacgaacta taatgacatc catatggggg
    12481 ttaaattttt tctgatcaaa atgtttcacg tgaaacaata atgttatgga ttttaaaatc
    12541 attgccattg atggtcctac cggtgttggg aaaagtacaa ttgcccgtca acttgcggaa
    12601 aaacttggct gtttgtatgt tgataccgga gcaatgtttc gttgccttgc tttgtgctgg
    12661 gggaagcatg gttgtccgga aagtgatgat tcgctgcaag agttgggtga tcagaccagg
    12721 atagtattta aacataaaaa tgtaatttgc aatggtaaag atgtaactga tgaaattcgg
    12781 acagaattga tttccagtct tgcttcaaaa atcagtagtt tttcctcaat ccgggaagtg
    12841 atgaaaaaac aacagcgcac attagttgaa gaagtccgta agtcagaaag ttacctgggc
    12901 gccgtattgg aaggtcgaga tattggcaca gttgtttttc cagatgcgga atttaagttt
    12961 tatgttgatg cctctccgga gatacgtgca aaacgtcgga tgctgcaact gcaggaacgt
    13021 ggtgaaaatg ctaattttga aaaaatatta acagctcttc gtaaacggga ttttcaagat
    13081 caaaaccgta aagttgctcc actgagacct gcggaggatg cattaattgt ggatacaggg
    13141 aatctgagtg cagaccaggt attggagcat ttagttgcct gtattggaga agagaaaaat
    13201 gtcagctagt ctggtgttca atccttaaaa aaccaaagaa tttatttaca aaggtgatgt
    13261 catgaaagca gttattcttg cggctaatcg aagtaacaga ttgagtccgt ttattgaaac
    13321 ccgctgtaag cccatgattc gaattgcggg gcaatatatt cttgaaaata cgatttgttt
    13381 cctcaaggaa gcagggatta acgacattgt gcttgtagtc aatcatcagc aggaccttgt
    13441 ccgccggtat tttggaagtg gcaaaacact tggagtgaat cttgagtatg ttgttcagga
    13501 tgaacttcag ggaattggtc aggccttgag tctttgccag tcgtcattgg aaaagaaacc
    13561 gtttttgctt gtttatggtg acgtaatgac tgattacaac ccctttttat ccatcctgca
    13621 aacttactct gagatggaca aggaagttgc cttgattgct ttaccggagt cttcaaaaga
    13681 gtttggcaat gtctatctgg atcatgaaat gaaaatcagc cgtttggttg aaaaaccaaa
    13741 ggtaaatcag gcaaattatg tttttgcagg aatgtttgtt ttgtttccga aatttttttc
    13801 tttgttggag gagcatcaga atgatatttc ccaatgttat gggcaaatga ttgaaaccag
    13861 cgggctacag gcttgtttgt gggaaaaagg atggattgat atgattcatc cctggcatat
    13921 tttggaagca aatcggatgt tgatgagttc atgggaaact gctgaaattg attctaccgc
    13981 aagattgctg ggacaagtgc atctcgaagg gtctgtccgg attgggccga atgttactgt
    14041 agaatccgga accatactta agggtccttg ttatataggt tccaattcat atgtggggaa
    14101 taattccctc atacgtaatt acagcgcgat tggtccggac tcaattgtag gctatggatc
    14161 tgaattgaaa aattgcgtgt tgtttggaaa caatgacgta ggccgtcttt cattcgtcgg
    14221 ggacagcgtt ctgggagaaa acgtcaaatc tggttccgga ctgacgacgg tcaatcacac
    14281 agtcgattat agtcggataa cctgcaagtc caactcagac tcaatagaaa ccggcctgac
    14341 aaagcttgga gcattcattg gtgataatgt atccattgga gcccgtcata ctcttgggcc
    14401 gggtacaatt gtggagtcag gaaaaatcgt tgctgataac attactctct aaatcaagct
    14461 cttaaaactt aaaacccggt aaggcaaaaa atgtgtggca tcagcggcat tgtccattct
    14521 gaaaatattg gcagaaattt atttaattca attcgcaact tggaatatcg tggatacgat
    14581 tcttgcggaa tggccttgct gcgacaagga gcacttgatt tgcgaaaaaa tgttggggga
    14641 attgatgaag tcaactcatt ggaaaaattg gatcaaatgc aggggagcat cggaattgct
    14701 catacgcgat gggcaacaca cggaggagta actcaggaaa acgcccatcc acacctttct
    14761 caaaacaagc agtttgtaat tgtacacaac ggtattattt caaattacca ggaattgcgg
    14821 gagcggatgc ttagtcaagg tgttgttttt gagtcattga ctgatacaga agttttcgtc
    14881 aacttgttgg aggaatcata ctcaaacagc aaagatatag aacaagcttt tttagcagca
    14941 cttgagcaaa tcgaaggttc attttctata gtcatgctgt cagcgcatga cccggaacac
    15001 ttgtattgtg taaaaaaaga gtcacctctg ttactgggac tgggagaggg ttgtaattat
    15061 gtcggttcag atctcaatgc ttttttggaa tacaccagga aggcggtagt tctggatgat
    15121 ggagaatatg ttgttttagg ccggaatgat tataaaatac gtaaattaaa aactcgtgaa
    15181 catgtcgaaa aaaatgtttt acacattgac tgggatgtgg aaacgagtaa aaagggcgga
    15241 tattcccatt acatgctcaa ggaaatattc gatgaaccgc agacaatacg gcaggcattg
    15301 aaaattccgc atgaacaaat ttcaggattg gccttgatgt ttacggaagc tacacgtgcc
    15361 tacctgatgg gtgtggggac aacttattat gtggcgaaca tcagtcagta ttttttttca
    15421 actttggcaa atcgatacta cccggcgttg agttctgacg agttttctgt agtaagtgtt
    15481 gaatccggag atatggtgct ggcactttcc caatccggag aaacctatga tacaaaatca
    15541 tcgttggagt atgcgaagct tcatggtgcg caaaccgcgg cggttgtaaa tgtgatgggg
    15601 tcggcaatca gtatgatggt agatcaggtt attatgcagg gttccggtcc ggaaatatgt
    15661 gttgtttcca caaaagctgc tttggctcaa accttcatta tgctgagagt agccttggaa
    15721 ctggggcgga tgcggaaaca tatttcgcag gatgtttatc aaaaacatca gcgggagttg
    15781 gagatatttc cgcaaatggt tgaccaaata ctcaatgaac aatccggatt cgtacgcaac
    15841 atagcaagga ctacaagtca tgtagagcat tggttatttc tcggctgcgg agtttattat
    15901 cctgtggcca tggagtctgc tttgaaaatg aaagaaatta cttatcaaca cgcagaagga
    15961 atgcctgccg gatttcttaa acacgggact ctgtcgatgg ttgaaccggc tttacattca
    16021 ctgttttttg taccgcttcc ggaacaaaaa gatttgcatg accgtactct tattgctatg
    16081 gaacaagtta aagctcgtgg aggtactgtg attggaatca tgtttcaggg tgacaaacac
    16141 gcccagtcag taaccgatca agcagtactg ctgcctcaga tgagtgcttt atgcgctcca
    16201 atggcacaaa tgattatggc acagatgttt gcatactatg ctgctttgga cttgggacgt
    16261 aatattgaca agcccagaaa tttggctaaa tctgtaacag tcggttaatc ggcttccctc
    16321 agctatcttt taatgtttgc taattagaat gaagatagta tcctcccttt taataagttc
    16381 tctttttcct gcaaacaaat gacgattgag cgtatacaag gaacggatgg tattcgcggc
    16441 attgtccgtc ttgcagagga ttgttccggt tcggatccaa tttctgtttt tctacatgaa
    16501 gctgtactga ctgaagagtt ttttgagttg tatacctacg cttattgtca ggaactgctt
    16561 gaagctgatt ttgcgtctgc aaatgatctt gctgtaattg gatgggatcc gagagatatg
    16621 agcggcagtt tcaatcactc cgctatccga ggaatcagga aggcaggtct tactgcggta
    16681 gttgtagata ttctacctac tccagccatt tccctttacc agctacacgt tggtgctgct
    16741 tgtgcttttg tactaacagc atctcataat cctgcagacc agaatgggat caaaatcttt
    16801 attgggcatt ccaatctgaa attatttccg gaagatgaca agcgcttgac gcaacgttgc
    16861 ctgagtttca attttgaaaa ccttcgatcc gctcccttaa ttggagaatt gcgcaatgat
    16921 catgcagctg cccggaagtt atttatagaa tttatggcag atccgcaaaa tcattggtta
    16981 aacaaagatt ctttagcagg tgctaaaata atcgttgatg cagcaaatgg tgctttttca
    17041 cccttaatcg aggatttgct gaactatgag tcagctgatt atatttttac taactgtgac
    17101 ccagcccggg gaattaacgt tcgttccggt gtagcagatc ttgaaggtgt atcttctatt
    17161 tctgcggatg agatcgaaaa tggtttgttt tctggatatg aaactttaga gaagatgctg
    17221 gcgagtggcc gtgaacaaaa ggaaaagctg ctcaacaact caggccaggt gctgggtttt
    17281 gtttttgatg gtgacggtga tcgttgtttt ttactgagct atgatccgtt tcaagattgt
    17341 gtgttggtag ttggtggcga cgcgcaggcc ttttttcagg ctaaattgct gcagcaaaac
    17401 tacagttgga atcagggacc actgtttgtg aatactgtcg aaagcgatct ggaagctgga
    17461 cgggcagtac agcagattgg ttttgaaaca atgcagtgtg cagttggtga caagtggatt
    17521 ttgtggcagg cctgttttta tgactggcag gcaaaacggg aattttatct aaacaaaatt
    17581 gctgcatctg aattcaggat tttgcaggag caagttagaa caaggctgga gcagatggtt
    17641 caaagtacta aatttgacgc tttgtctgca actactactt ttatgtctct tgaaaaatgg
    17701 gttagtaata atattgggaa tgaactggtg aatagcgcgt acgaccacgt atgccagcag
    17761 cagaacaata tttttgcaat cggcagtgaa gagtccggac atgttattac tttagctaaa
    17821 atgaacttcg gtcacgtaaa ccgtccggta tttatcggca atggattgaa atgcgccttg
    17881 aatagtctgg cggcaattca ggctttacgt tccgcaaaaa atactcctga attctttgaa
    17941 tggctgaaat gtccctttcc ttccggattt caaaaaagtc ttccggttta ttatgttgac
    18001 aaatccttgc tggatgaagg atctgtgctg cgtactgagc tggaggaatt gttactggca
    18061 aacttaaact ggccggaaat ggaagttgaa attgtgaaac gccaggagga accagaaatg
    18121 ctgctgctga atgttttaga aaatggactt ccagcagcgg ctgtatttgt gcggaattcc
    18181 ggaacagagg ataagctggc gctttatctc cggggaagga aggaactcga agtacgcctg
    18241 gaaagccttg cggaaaaaat ttatcccttt ttgttgcttt cgtttaaaaa taaaaatagc
    18301 cctatggcac aggccgagca attggttttg ctccgcttga aagatggagc aaaacaggca
    18361 atcgatttga gttttgaaca atttgctaag atctccctga atcgactgct tcacgaaatg
    18421 agctcacgtc agcaactgat taagcaggaa ggagacactt ggtccatcac ggaaactgga
    18481 aggatatgtt tgaatcatac tgaacgttca gaatgattat ctttaatcaa ggatttttct
    18541 gaacgttcag aaaacacatg tcatctaaat ag
//
LOCUS       DTVD01000226           18466 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_531_length_18466_cov_181.735756, whole genome
            shotgun sequence.
ACCESSION   DTVD01000226 DTVD01000000
VERSION     DTVD01000226.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 18466)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18466)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18466
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_18466_cov_181.735756"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="rimO"
                     /locus_tag="EYO68_07995"
     CDS             complement(<1..1262)
                     /gene="rimO"
                     /locus_tag="EYO68_07995"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIC07784.1"
                     /translation="MTSPVAESVSPSHEKKRIYLETLGCSKNQVDSEIMLASLQNQGF
                     EMTKTPDSAEVIIVNTCAFLTEASEESINRILELSDFKSTGNCEKLVATGCLTQRYQD
                     SLAEQIPELDGLFGSNGFEQIPELVYSMYEGTGQTKLNLKQKPHYRQFEKQARIQTTP
                     RHYTYLKVAEGCSNMCSFCNIPALRGNFSSRTVNSIVTEIINLTKKGVKEINLISQDT
                     SSYGKDFKNGTGLATLLKNISKIEGDFWIRLFYCYPNSFTDEVMEVIAGDKRFCRYLD
                     MPFQHINNEVLKAMNRKIDRAQIENKIQEIQKHLPDLAWRTTFIVGFPTETEDHFQEL
                     LEFVSAGHFMHVGVFSYSHEDNIRSAKWGDPVPNSLKQERRKQLMEAQQKVSSGRNKS
                     WIGKQLKVLVDGLSEESDLLLQGRSEFQG"
     gene            complement(1362..2225)
                     /locus_tag="EYO68_08000"
     CDS             complement(1362..2225)
                     /locus_tag="EYO68_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIC07785.1"
                     /translation="MRHLKAGNRLGVTTAHRRAMMRNMVTSILENGQITCTLARAKEV
                     RKPLEKMIGWGKQGSLHARRQALSFVKSKEAMTQLFEELAERYKDRQGGYCRIIKLGK
                     NRLGDNSEMAIVQLLGSETDRLSELKSQSGKKKKAKKKESPVLKEVSEEIRTENSSSE
                     KNDESVKPIEAEPTLAEGEEVETKKEISQVENETSKEESPVESVQIEPAAKQKPEENI
                     QTETVSEEQLEADTKKEEPVTTETDSAEDEAKTEPEKEEVQADSEAVQEETEITSEKV
                     ITEEKDPETKT"
     gene            complement(2240..3367)
                     /locus_tag="EYO68_08005"
     CDS             complement(2240..3367)
                     /locus_tag="EYO68_08005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIC07786.1"
                     /translation="MTELNETIEEADPEAENVSPEDNAITGTESDSNSEEESTDENPF
                     FAKNWMSIAKPKKLQFESENLKSDYAKFTLDPLEPGFGTTIGHSLRRVLLSSIRGSAV
                     FAVQIDGVTHEFSNIPGIVEDMVQVILNIKELQVEQFVDEVVELELIGEGPCVIKAGD
                     ISTFEKAEILNPDLVLATLQKGATVSMTMYSCFNKGYVTSEENQQEDLPVGTIYLDSN
                     HSPVSRINYDVVNSRVDQKTDYDRLNFELWTNGSVKPTDCLAYAAKIIKEHMDVFINF
                     DESSIKDEPEEEIEEEPLNENLYRSVSELELSVRSINCLQNAKIETIGDLVQKSEQAM
                     LKTKNFGRKSLNEIKVILTEMGLSLGTELENFDPMNNPHTN"
     gene            complement(3473..3856)
                     /gene="rpsK"
                     /locus_tag="EYO68_08010"
     CDS             complement(3473..3856)
                     /gene="rpsK"
                     /locus_tag="EYO68_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIC07787.1"
                     /translation="MAEAKKKKEKRTVEQGRIYIKATFNNTIVTITDTQGNTIAWSST
                     GVHGFKGSRKSTPFAARVAADDALKKAVDSGMKSVDVFVKGPGSGRESALRAISAIEG
                     MRVTSINDITPIPHNGCRPPKQRRI"
     gene            complement(3884..4252)
                     /gene="rpsM"
                     /locus_tag="EYO68_08015"
     CDS             complement(3884..4252)
                     /gene="rpsM"
                     /locus_tag="EYO68_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIC07788.1"
                     /translation="MARISGIDLPNTKRVEIGLTYIFGVGRKVSNDILLKAKIDKNVR
                     VSDLDDDQVNKIRTIIEKEYQVEGDLRREVSLNIKRLMDLGNYRGLRHRKHMPVRGQR
                     TKTNARTRKGPRRLAVSKNK"
     gene            complement(4264..4377)
                     /gene="rpmJ"
                     /locus_tag="EYO68_08020"
     CDS             complement(4264..4377)
                     /gene="rpmJ"
                     /locus_tag="EYO68_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIC07789.1"
                     /translation="MKVRSSVKKICEKCKIIKRKGVLRVICENPKHKQRQG"
     gene            complement(4396..5724)
                     /gene="secY"
                     /locus_tag="EYO68_08025"
     CDS             complement(4396..5724)
                     /gene="secY"
                     /locus_tag="EYO68_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIC07790.1"
                     /translation="MLNIIQNIFRIEELRQRIFFTLAMLFIFRLGAHIPTPGIDGAAL
                     TAFFEAQQGSILRFFDMFTGGALARLTVFALGVMPYISASIIIQLLTAVFPYLERLSK
                     EGDAGRKKITGYTRYGTIVLSVVQGFAIAVGLESMAAPNGQPIVISTLSPWAFRALTV
                     ITLTAGTAFLMWVGEQISERGIGNGISLLIFAGIVVGIPGAIINSVRLVQDNVLSPLV
                     LAVVGILMIVVVFLVIFAETAYRKIPVQYAKRMQGNRMYGGQSSHLPLKINTAGVIPP
                     IFASSILAFPATITGFIAVPWVQAVGSQLLPGRMLYNFLFAIMIFFFAFFYTAITFNP
                     VKIAEEMKKHGGYIPGIRPGKKTAEYVNLVLTRLTFGGAAYLAVVCLFPYILFDLFDI
                     PFYFGGTALLIVVGVGLDLVNQIEAFLLNQNYDGFMKKTKRRQMSAALRL"
     gene            complement(5735..6181)
                     /locus_tag="EYO68_08030"
     CDS             complement(5735..6181)
                     /locus_tag="EYO68_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIC07791.1"
                     /translation="MYLHDLESSKNKRRKRVGRGPGSGSGKNCGRGQNGAKSRSGYKR
                     KRGFEGGQNPLNRRLPKFGFTSPNKVYAQLINLQNLEDNADVETGSSLDKTKLKALGL
                     IKKEDKPVKLLGKGKLSKKLNIEVDMTSASAAEAVKKAGGEVVTIG"
     gene            complement(6199..6384)
                     /gene="rpmD"
                     /locus_tag="EYO68_08035"
     CDS             complement(6199..6384)
                     /gene="rpmD"
                     /locus_tag="EYO68_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIC07792.1"
                     /translation="MASKIKVTQIRSRIGAQKKQKACLSGLGLRKINNFSVLENTPAV
                     RGMIKKVSHLVRIETAG"
     gene            complement(6387..6887)
                     /locus_tag="EYO68_08040"
     CDS             complement(6387..6887)
                     /locus_tag="EYO68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIC07793.1"
                     /translation="MAEKNREKNELIEKVVRVNRVAKVVKGGRRFSFSALIVVGDGRG
                     QVGLGLGKANEVVEAIRKAVEQASVNMKKICVEGTSIPHVAIGRFGSGKVLMKPADKG
                     HGIIAAGAVRAVMEAAGIMDISVKCIGSRNPNNLVRSTLDGLYNLRSNDWIARARGKD
                     IDYLKQ"
     gene            complement(6919..7287)
                     /locus_tag="EYO68_08045"
     CDS             complement(6919..7287)
                     /locus_tag="EYO68_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIC07794.1"
                     /translation="MNNAATRLKGRETRHRRIRKKISGTNTRPRLAVFRSARHIYVQA
                     IDDNAGSTLAASSTVAIQKSLKKIYTGNKEAAKAVGADVANKLMEKSISSVVFDRGGF
                     IFHGRVQALAEGAREAGLKF"
     gene            complement(7308..7847)
                     /locus_tag="EYO68_08050"
     CDS             complement(7308..7847)
                     /locus_tag="EYO68_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIC07795.1"
                     /translation="MSRIGKRPIKLTQGVEVNFKERLLSVKGPKGELELRAHPAIELI
                     IDTENISVLPSDLSAPMTPMLGTTRALISNMIHGVTEGFEKQLNLVGVGYKAVVNQKS
                     LLLNLGYSHSINYTLPEGIKASVNANTKILLESSDKQLLGQVSAEIQKFRPPEPYKGK
                     GILFEGVQIKRKAGKSGKT"
     gene            complement(7871..8266)
                     /gene="rpsH"
                     /locus_tag="EYO68_08055"
     CDS             complement(7871..8266)
                     /gene="rpsH"
                     /locus_tag="EYO68_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIC07796.1"
                     /translation="MSMSDPIADLLTRIRNANMRKKESLEMLSSKMKLNIVKVLKDEG
                     FIVNWELDEKGKFPELRIWLKYVDDLPVIRKITRISKPGLRLYSKGKDCKPVLNGQGI
                     SILSTSKGIMSDRQCRADDIGGEVLCTVC"
     gene            complement(8299..8484)
                     /locus_tag="EYO68_08060"
     CDS             complement(8299..8484)
                     /locus_tag="EYO68_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIC07797.1"
                     /translation="MAKKSMIAKSERTQKFKVRQYSRCGYCGRPRGYLRKFGMCRICF
                     RKNANAGLLPGVTKASW"
     gene            complement(8490..9035)
                     /gene="rplE"
                     /locus_tag="EYO68_08065"
     CDS             complement(8490..9035)
                     /gene="rplE"
                     /locus_tag="EYO68_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIC07798.1"
                     /translation="MSESHLLQTYNSKVVPLLQEKFKYKNKHQIPAINKVTLNIGLGE
                     AVQNPKAVEAASKQLALISGQKPVVTKAKRSIAGFKLREGMPIGCMVTLRNVQMWTFL
                     DKLLHIAMPRIRDFRGISPKAFDGAGNYTMGIKEQLIFPEIEIDDVDNIRGMNISIVT
                     SALTDEEGLAMMQDLGFPFRK"
     gene            complement(9035..9376)
                     /locus_tag="EYO68_08070"
     CDS             complement(9035..9376)
                     /locus_tag="EYO68_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIC07799.1"
                     /translation="MKAKQRKKRRATIRREDTVMVISGKDKGRTGRVITVDWKRERLL
                     IEGVNMVTRHTKPNAANQKGGLIKIEAPVHYSNVQLYNPTLERGVRVKIQTSDAGVKN
                     RVCVKSGEVLG"
     gene            complement(9388..9756)
                     /gene="rplN"
                     /locus_tag="EYO68_08075"
     CDS             complement(9388..9756)
                     /gene="rplN"
                     /locus_tag="EYO68_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIC07800.1"
                     /translation="MIQTQSILEVADNSGAKRLMCIKVLGGSKRRYARVGDIIVASIK
                     DCQPHSKVKSGDVVKAVVVRTKKESRRPDGSYIKFDSNSAVLIDGKREPVGTRIFGPV
                     ARELRAGKFMRILSLAPEVL"
     gene            complement(9753..9992)
                     /gene="rpsQ"
                     /locus_tag="EYO68_08080"
     CDS             complement(9753..9992)
                     /gene="rpsQ"
                     /locus_tag="EYO68_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIC07801.1"
                     /translation="MSTLKLQSGIVVSNKMDKSIVVKIERKIKHPLYKKTIKRSKKYI
                     AHDEQNECKVGDLVQIAECRPLSRRKRYRLFKPSK"
     gene            complement(10009..10203)
                     /gene="rpmC"
                     /locus_tag="EYO68_08085"
     CDS             complement(10009..10203)
                     /gene="rpmC"
                     /locus_tag="EYO68_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIC07802.1"
                     /translation="MNASELRALPLNELEAKKYDLKEEYLRLRCGHVSRQLADVNLIK
                     KARRNIARLNTVLTEKLAKQ"
     gene            complement(10200..10616)
                     /gene="rplP"
                     /locus_tag="EYO68_08090"
     CDS             complement(10200..10616)
                     /gene="rplP"
                     /locus_tag="EYO68_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIC07803.1"
                     /translation="MLMPKKTKFRKQMRGRMRGDAHTGNKISFGSIGLQATGRGYVTT
                     RQIEAARRVITRTLKRGGKTWIRVFPDKPVTKRPAETRMGKGKGAVDTWVASVHPGRV
                     LYEIDGVPEEVATEALRLAAYKLSINTKIIKRAAVI"
     gene            complement(10629..11282)
                     /gene="rpsC"
                     /locus_tag="EYO68_08095"
     CDS             complement(10629..11282)
                     /gene="rpsC"
                     /locus_tag="EYO68_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIC07804.1"
                     /translation="MGQKVNPIGLRLGISRTWNSKWFAEKDYASQLRQDLDIQKFVKA
                     RLKHAAISKIEVERTAKRTKVNIFSARPGIIIGRKGDEVERLKKELSKRNYNDVTINI
                     KEIKRPEIDATLIAENIASQLERRIAYRRAVKRSIQAAMRMNIAGCKVMVGGRLNGAE
                     IARSEWVREGQVRLHTLRVDIDYGVAEANTTYGIIGVKVWVYRGEDYGKSRGRRSFS"
     gene            complement(11344..11676)
                     /locus_tag="EYO68_08100"
     CDS             complement(11344..11676)
                     /locus_tag="EYO68_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIC07805.1"
                     /translation="MTAQATARNVRVAPRKARLVVDMIRGQNVVRAMAQLSTIRNRAA
                     EPIMKVLQSALANADQHHPEADVDNFRVITAYVDEARMLKRFRPRAMGRASGIKKRSS
                     HITLEVGE"
     gene            complement(11676..11957)
                     /gene="rpsS"
                     /locus_tag="EYO68_08105"
     CDS             complement(11676..11957)
                     /gene="rpsS"
                     /locus_tag="EYO68_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIC07806.1"
                     /translation="MPRSLKKGPFVDAHLEKKVAAAKGNNDRRLIKTWSRRSVILPDF
                     IGTNIAVHNGNKFIPVFITEDMVGYKLGEFAPTRTYRGHVGKSDKRVRR"
     gene            complement(11961..12779)
                     /gene="rplB"
                     /locus_tag="EYO68_08110"
     CDS             complement(11961..12779)
                     /gene="rplB"
                     /locus_tag="EYO68_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIC07807.1"
                     /translation="MGIKFHKPTSPGRRGMSGFVFEEITASTPEKSLLAPLKKKGGRN
                     NNGRITVRHHGGGHKRRYRIIDFSRNKQEIPAKVASVEYDPNRSARISLLHYADGEKR
                     YILTPFGLKVGDVIFSGENADIKTGNALPLKNIPVGTMLHCIELRPGKGAQLGRSAGA
                     AIQLMAKGDDYAQLKLRSGEIRKVRIECMATVGTVGNAEHMNINKGKAGRNRWLGRRP
                     TVRGVVMNPVDHPHGGGEGKTSGGRHPVTPWGKPTKGAKTRKRKDTDKYILKRR"
     gene            complement(12797..13084)
                     /locus_tag="EYO68_08115"
     CDS             complement(12797..13084)
                     /locus_tag="EYO68_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIC07808.1"
                     /translation="MNIFDVLRAPKMSEKTLSLKEEANQFAFEVDQRANKIQIKESIE
                     KSFKVSVLKVRTMNVRGKKKRLGRYQGLKSSWKKAIVTLKEGDTIEYFEGA"
     gene            complement(13081..13707)
                     /gene="rplD"
                     /locus_tag="EYO68_08120"
     CDS             complement(13081..13707)
                     /gene="rplD"
                     /locus_tag="EYO68_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIC07809.1"
                     /translation="MATLKTVDAGNKAAGKIEIDDTILETPYHRKAVSEVVRQFLAAN
                     QQGTHSTKNRAEVAYSTRKLYRQKGTGSSRAGSAKSPIRRHGGTIFGPQMRSHSFRLN
                     KKTKRLATRSVFAEKVRQDGILVLDSLNLTDNKTKNLKAMLTKLELPEKVLIVADEVS
                     ENLRLASRNLPDVHVLSFRSLNVYEMMKYTKVVFLKDALEAYKERLAR"
     gene            complement(13729..14043)
                     /gene="rpsJ"
                     /locus_tag="EYO68_08125"
     CDS             complement(13729..14043)
                     /gene="rpsJ"
                     /locus_tag="EYO68_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIC07810.1"
                     /translation="MMEQKIKVRFKAYDEKLLDKTIGEIVHTVKRTGAEIVGPIPLPT
                     RKNSYTVLRSPHVDKKSREQFEIRTHKRLLYILETTPQTMDALMRLDISAGVDIAIKQ
                     EG"
     gene            complement(14057..15256)
                     /gene="tuf"
                     /locus_tag="EYO68_08130"
     CDS             complement(14057..15256)
                     /gene="tuf"
                     /locus_tag="EYO68_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIC07811.1"
                     /translation="MAREKFDRSKPHVNVGTIGHVDHGKTTLTAAITKVLQMDGLAEA
                     KGFDEIDGAPEEKERGITIATSHIEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     IIVVSAADGPMPQTREHILLARQVNVPHLVVFLNKIDQVDDEELLELVEMEVRELLDY
                     YDFPGDDTPIIKGSALAVMESSATDSSDPAFVCIKELLDAIDSFIPEPVRDLDKDLLM
                     SVEDVFTISGRGTVATGQIERGIVKVGEDIEITGIRETQTTTVTGVEMFRKSLDQGQA
                     GDNVGILLRGIKREDIERGQVICKPGSITPHTKFKGQVYVLDKDEGGRHTPFFTNYRP
                     QFYFRTTDVTGVVTLPSGIEMVMPGDNVEMTIELICPIAMEKEVRFAIREGGRTIGSG
                     VVTEVIE"
     gene            complement(15303..17396)
                     /gene="fusA"
                     /locus_tag="EYO68_08135"
     CDS             complement(15303..17396)
                     /gene="fusA"
                     /locus_tag="EYO68_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIC07812.1"
                     /translation="MARTVPLKQRRNLGIMAHIDAGKTTTTERILYYTGRIHKIGEVN
                     DGSATMDWMEQEQERGITITSAATTCSWETDKKEYVLNIIDTPGHVDFTAEVERSLRV
                     LDGSVMVLCAVAGVQPQTETVWKQAKRYNVPCIAFVNKMDRVGADFEKAVISIEDQLS
                     ASPVAIQIPIGAEEEFTGVIDLVSMQELRFKDEGFGEEYESAELSDELRASAQDWRRQ
                     MLEHLSLFDDDLLEKIVEEQQVEQEEIVKVLRKATLSGKITPVLCGSAFKNKGVQQLL
                     DAVVNYLPSPFDIPPVQGDHPVTKKPVSREASESDPLCALAFKVASDPFAGQMTFVRV
                     YSGSFETGKTVFNPRKRKHERIGNLVKLHANKREDISVIKAGDIGAVIGMELITGDTL
                     CPKDHPIILERTQFSEPVIDQAIEPKTKQDQDKLEEALKRLMQEDPSFRSRIDAETGQ
                     NIISGMGELHLEVMVHRLQREFQIDCKAGTPRVAYRETITQAADSETRFEQQLDGKDQ
                     FAFCRISLEPLEAGGGFEFENKLSAEELPTQFAEALEQGIQDAMSNGVLAGFSLIDLK
                     VCLVAGEYLEESSTEVAFTIAGVNALREGVRKAGPALLEPSMKIEVVTPVSFTGEVIG
                     DLNARHGRIRGMEEQGGIQVVEAEAPLAEMFGYSTRLRSLTQGRASFTMHFSHYTSVT
                     ESTRKILIGEPVSLG"
     gene            complement(17447..17920)
                     /gene="rpsG"
                     /locus_tag="EYO68_08140"
     CDS             complement(17447..17920)
                     /gene="rpsG"
                     /locus_tag="EYO68_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIC07813.1"
                     /translation="MPRRRAATKRVILPDPKYKDLLVSKFINSLMKHGKKSIAENIFY
                     SALDIISERESEMSSLEIFKAAIENVMPSVEVKSRRVGGSTYQVPLEVRHSRSQSLAI
                     RWLIENANARSGLSMRAKLADEFADASNSRGSAVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(17930..18301)
                     /locus_tag="EYO68_08145"
     CDS             complement(17930..18301)
                     /locus_tag="EYO68_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIC07814.1"
                     /translation="MPTLSQLVRKGRKSKLTKKDTPALEACPQRRGVCVRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGIGHNLQEHSVVLIRGGRIKDLPGVRYHVVRGKL
                     DTVGVNERKQSRSKYGVKKDQ"
BASE COUNT         4618 a         4366 c         3382 g         6100 t
ORIGIN      
        1 ccttgaaatt cacttcggcc ttgaagtagc aagtcacttt cttcactaag gccatccaca
       61 aggactttca attgttttcc aatccagctt ttatttctac cactgctgac tttttgttgt
      121 gcttccatca gttgcttacg ccgttcttgc ttaagagaat ttggaacagg atctccccat
      181 tttgctgaac ggatattatc ttcatgggag tatgaaaata ctccaacatg cataaaatgt
      241 cctgctgaaa caaactcaag taattcctgg aaatgatctt ctgtttcagt aggaaagcca
      301 acaataaaag tagtacgcca agccaaatcc ggaagatgtt tctgtatctc ctgtatttta
      361 ttttctatct gagcacggtc tatttttcgg ttcattgctt tcaagacttc attattaata
      421 tgttgaaacg gcatatcgag ataccggcaa aatcttttat cacctgcaat cacctccatc
      481 acttcatcag taaaggagtt gggatagcag taaaaaagac gtatccagaa atccccttct
      541 attttactga tgttttttaa aagtgttgcc agtccagtac cgttcttaaa atcttttccg
      601 taagatgaag tgtcttgcga aatcagattg atttccttca ctcctttttt tgtcagatta
      661 atgatttcag ttacaataga gttgactgta cggctactga aatttcctct taacgcaggt
      721 atgttgcaaa acgaacacat gttggaacag ccctccgcca ctttaaggta tgtataatga
      781 cgaggtgtag tttgtatacg ggcctgtttt tcaaattgtc tgtagtgagg tttttgcttc
      841 aaattaagtt ttgtttgtcc ggtaccctca tacattgagt acacaagttc cggaatctgt
      901 tcaaatccgt ttgaaccaaa cagtccatcc agttctggaa tctgctccgc gagactgtcc
      961 tggtaacgtt gagtcaaaca acctgttgcg acaagctttt cacagttgcc agtacttttg
     1021 aaatcactta gttcaagaat cctgttgatt gactcttcac ttgcttctgt aagaaaagca
     1081 caggtgttga caataattac ttctgcagaa tccggagtct ttgtcatctc aaaaccctgg
     1141 ttttgcagac tggccagcat tatttctgaa tcaacttggt ttttggagca acccaatgtt
     1201 tccaaataaa ttcgcttctt ttcgtgcgaa ggcgatacag attctgcaac aggacttgtc
     1261 atagtgaaca gtttttttct agaagtacaa atggtaggag taaaaaaaga ctcctgtaga
     1321 aaaaagaagc ctttaaagga gggattactt gaaggtcagt cttaagtttt tgtctcagga
     1381 tccttttctt cagttataac tttctcggaa gtgatctcag tttcttcctg tactgcttca
     1441 gaatctgcct gtacctcctc tttttcaggt tctgttttag cttcatcctc tgctgaatca
     1501 gtttcagttg taacaggttc ttcctttttt gtatcagctt caagttgttc ttcagaaaca
     1561 gtctctgtct gaatattctc ttccggtttt tgctttgctg ccggttctat ttgaacagat
     1621 tctacaggtg attcttcttt tgaagtttca ttctcaactt gagatatttc tttcttcgtt
     1681 tctacttctt caccttcggc caaagtaggt tccgcttcta taggtttaac tgattcgtca
     1741 tttttttctg aagatgagtt ctcagtccgg atttcttcac ttacctcctt taaaacaggg
     1801 ctttcctttt tcttggcttt tttcttttta cctgactgag atttaagttc gcttaatcgg
     1861 tcggtttcac ttccaagcaa ctgtacaatc gccatttcag agttgtcacc aagtctgttt
     1921 ttacccaatt tgataattcg gcaataacca ccttgtcgat ctttgtagcg ctcagccaat
     1981 tcttcaaaaa gctgtgtcat tgcttctttg cttttcacaa aactcaatgc ttgacggcga
     2041 gcatgcaaac tgccctgctt tccccagcct atcatttttt caagaggctt gcgcacctct
     2101 tttgccctgg caagagtgca ggtaatctgg ccattttcca gaatagatgt aaccatattc
     2161 cgcatcattg cacggcgatg tgccgttgta actcctaaac ggtttccagc ttttaaatgt
     2221 cgcatcgtat tattgagccc taatttgtgt gtggattatt cattggatca aaattttcca
     2281 gttctgttcc caatgataag cccatttctg tcaagattac tttaatttcg ttcagtgatt
     2341 tccgaccaaa gttttttgtt ttgagcattg cctgttcact cttctggaca agatctccta
     2401 tggtttcaat ctttgcattt tgcagacagt tgattgaacg gacagacaat tccagttcgc
     2461 taacactgcg atataagttt tcgttcagtg gttcttcctc aatttcttct tcaggctcat
     2521 cctttatgct gctttcatca aagttaataa acacatccat gtgttctttg attatttttg
     2581 ctgcataggc caggcagtcg gtaggtttta cagaaccatt tgtccatagt tcaaaattca
     2641 ggcgatcata gtctgttttt tgatcaactc ttgaatttac aacgtcatag ttaattctac
     2701 tgacaggcga gtgatttgag tcaaggtaga tggtgccgac gggtaaatcc tcctgttgat
     2761 tttcctctga ggtaacataa cctttgttga agcaggaata catagtcata ctgacagttg
     2821 cccctttttg taaggtagcc aaaacaagat ctggattcaa tatttctgct ttttcaaagg
     2881 ttgaaatatc tccagctttt attacgcaag gtccttctcc gatcagttcc agttcaacaa
     2941 cttcatcaac aaattgctca acttgaagtt ccttgatatt aaggataact tgaaccatgt
     3001 cctctacgat tccagggatg ttgctgaatt cgtgggtgac accgtcaatt tgtactgcaa
     3061 aaactgctga tcccctgatt gaagacagga gaacccgcct taaagaatgc ccaattgtcg
     3121 ttccaaatcc aggctctaat ggatccagtg taaacttcgc ataatcagat tttaagtttt
     3181 ccgactcaaa ctgtagtttc ttcggctttg caattgacat ccaattttta gcaaagaacg
     3241 gattttcgtc cgttgattcc tcttcactgt tggaatccga ttctgttccg gttattgcat
     3301 tatcttccgg agagacattc tctgcctccg gatctgcttc ttctatagtt tcatttaatt
     3361 cagtcatctg catcccagtt aagttttctg gttttgattt agtcaaaaaa gacaatacta
     3421 atcgatgaaa taatcatcct gagagtattg taattaatga cgtttaatta aattaaatcc
     3481 gccgttgctt tggtggacgg catccgttat gaggaatcgg tgttatatcg ttgattgacg
     3541 tgacacgcat tccttcaatt gctgaaattg ctcgaagtgc agactcccgt cctgaaccgg
     3601 gtcctttgac aaaaacatca acacttttca taccactatc aacagccttt ttcagggcgt
     3661 cgtctgcagc cactcttgct gcaaaaggag tactttttct tgaaccttta aatccatgta
     3721 cacctgtact agaccatgcg attgtgttgc cttgagtatc ggtaattgtc acgattgtat
     3781 tattgaatgt ggccttgata taaattcgac cctgttcaac tgttcttttt tcttttttct
     3841 tttttgcttc cgccataagt tcggctcaat tcaacgttta ggtttattta tttttactga
     3901 ctgccaatct tcgggggcct tttcgcgtcc ttgcatttgt tttagtcctt tgtcctctga
     3961 caggcatatg cttgcgatgc cttaatccac ggtaattacc caaatccatt agacgcttga
     4021 tattcaagct gacttctcgt cgaagatcac cctcaacctg atattctttt tcgataattg
     4081 tacgaatctt atttacctgg tcgtcatcaa ggtcactaac tcgtacattt ttatcgatct
     4141 ttgctttgag taatatatca tttgaaactt ttctgcctac accaaaaatg taagtcaaac
     4201 caatttctac ccttttagta tttggtaaat caataccgga aattcttgcc atccagctcc
     4261 ttgttatccc tgtcgctgtt tatgttttgg attctcacaa attacccgca agacgccttt
     4321 tcttttaatg attttgcact tttcgcagat tttctttaca gatgaacgta ctttcatttc
     4381 attttcttca aagtatcata aacgcagtgc tgcagacatc tgtcgtcgtt tagtcttttt
     4441 catgaagccg tcataatttt ggttcagcag aaaagcttca atttgattaa ccagatctag
     4501 tcctactcct accacaatta atagtgccgt ccctccaaaa tagaaaggaa tgtcaaaaag
     4561 atcaaaaagg atgtatggga aaaggcaaac aacagccaag taagctgccc ctccaaatgt
     4621 taatcgtgtt agaactaagt taacatattc agctgttttt ttacctggtc gaatacccgg
     4681 tatgtatcca ccatgtttct tcatctcttc tgctatcttt actgggttaa atgtgattgc
     4741 cgtgtaaaaa aaagcaaaaa agaagatcat aattgcaaac aaaaaattat acaacatcct
     4801 gcctggtagc aactgactgc cgactgcctg cacccacggt acagcaatga agccggtgat
     4861 tgttgccggg aaagcaagga tcgaagatgc gaaaattgga ggtataactc cagcagtgtt
     4921 tattttcagg ggaagatgag aagactgtcc cccgtacata cgattcccct gcattcgttt
     4981 tgcgtattgt actggaatct ttctgtaagc agtctcagca aaaataacca ggaatacgac
     5041 tactatcatc agaataccga caactgcaag tactaatggt gacaatacat tgtcctgcac
     5101 caaacggact gaatttataa ttgcgcccgg gataccaacc acaattcctg caaaaatcaa
     5161 taacgaaatt ccatttccaa ttccacgttc actaatctgc tcgcctaccc acatcagaaa
     5221 agctgtacca gctgttaatg tgattacagt tagtgccctg aaagcccatg ggcttaatgt
     5281 cgaaattacg attggttgtc catttggtgc cgccatactt tccagtccaa ccgcaatggc
     5341 aaaaccttga accacactga gaacaattgt tccgtatctg gtataacctg taatcttttt
     5401 tcgaccggca tcgccctctt ttgataatct ttcaaggtag ggaaatactg cagtcaataa
     5461 ctgaattatg attgaggcac taatgtaagg catcacaccc agtgcaaaaa cggtcaatcg
     5521 tgccagtgct cctccggtaa acatatcgaa gaagcgtaat atagaacctt gttgcgcctc
     5581 aaaaaaagct gtcagagcag ctccgtctat tccgggagta ggtatatgtg caccaaggcg
     5641 aaaaataaaa agcattgcca gagtgaaaaa aatacgttgc ctcagctctt caattctaaa
     5701 aatgttctga attatattta acatcaaaga agccttagcc tatcgtaacc acttcaccac
     5761 cagctttttt tactgcttct gcagcacttg cgctagtcat gtcaacttct atatttaatt
     5821 ttttgctcag tttgccctta cctaaaagct ttacaggctt gtcttctttt ttgattaaac
     5881 ctaaagcttt taattttgtt ttatcaaggc tgcttcctgt ttcgacatct gcattatctt
     5941 ccaggttttg taaatttatc aactgagcat acaccttgtt aggacttgta aatccaaact
     6001 taggcaatct acggttgagt ggattttgtc caccttcaaa tcctcgcttc cttttatagc
     6061 cggatcgtga tttcgcaccg ttttgtccgc gtccgcagtt ttttccgcta ccgctgcctg
     6121 ggccacgtcc aacacgttta cgacgtttgt ttttactgga ttcaagatcg tggagataca
     6181 tatattaccg taattctatt aacctgctgt ctcaatcctg acaagatgac taaccttttt
     6241 gatcatccct cgaactgccg gagtgttttc aagcactgaa aaattgttta ttttccgtaa
     6301 tcccaatcca cttagacatg ctttttgttt tttttgtgcc ccaatcctac tgcgaatctg
     6361 ggttactttt atcttactgg ccatttttat tgtttcaaat aatcgatgtc tttgcctctt
     6421 gctctggcaa tccaatcgtt actcctgaga ttgtataatc catcaagtgt tgagcgtacc
     6481 aggttgtttg gatttctgga accaatgcac ttaacactga tgtccatgat accagctgct
     6541 tccataacag ctctaacagc accggctgca ataattccat gtcctttgtc tgcaggtttc
     6601 attaaaacct ttccggatcc aaaacgacct attgctacat gtggtattga tgtgccttcc
     6661 acacatattt ttttcatgtt aaccgaagcc tgttcaactg ctttacgaat tgcctcaaca
     6721 acctcgttag ccttcccgag accaagacca acctgacctc gcccatcacc aacaacgatt
     6781 aatgcgctaa agctgaatct acgaccgcct tttaccacct tggcaacacg gtttacgcga
     6841 actacttttt ctatcagctc attcttttct ctattttttt ctgccactac ttcaccatta
     6901 ttccggtttt accggttttc agaatttcag gccagcttcc ctagctcctt cagctaaggc
     6961 ctgtacacgt ccatgaaaaa taaatccacc gcggtcgaag acaacggagg aaatggactt
     7021 ttccataagt ttatttgcta cgtctgcgcc aaccgcttta gctgcctctt tgttccctgt
     7081 gtagattttt ttcaaggact tctggattgc tacagttgaa gaagctgcca aagtacttcc
     7141 tgcattatca tcaatagcct gcacataaat atgtcgtgca gatctgaata ccgctagtct
     7201 aggacgcgta ttagttccag aaattttttt tcgaatacgt ctgtgtctag tttctcttcc
     7261 tttcagacgt gttgcagcat tattcatttc aagctcttac ttgcgtatta agttttgccg
     7321 cttttaccgg ctttacgttt tatttgaaca ccttcaaaca aaattccttt tcctttgtat
     7381 ggctcaggtg gtctaaattt ttgaatctct gcagaaacct gccccaaaag ctgtttatcg
     7441 gaactttcaa gaagtatctt agtatttgca tttacagatg cttttattcc ttccggcaac
     7501 gtgtaattta ttgagtgtga atagcccagg tttaataaca aacttttctg atttacaact
     7561 gctttgtatc cgacacctac caaatttagc tgtttttcaa aaccttctgt gacaccgtga
     7621 atcatattac taatcagtgc ccgggttgtt cctagcatag gtgtcattgg agcactcaga
     7681 tcagaaggca acactgaaat attttcagta tcaattatta actcaatcgc aggatgtgcc
     7741 cttagttcca gctcgccttt aggacccttc actgaaagca ggcgctcctt aaaatttact
     7801 tcaactccct gtgttagttt aatgggtctt ttaccaattc ttgacattgt ctttcttgat
     7861 taaatacagt ctaacagacc gtacaaagta cttccccacc aatatcatcg gcacggcact
     7921 gtcggtcact cattattcct tttgaggtag aaaggataga aataccttgc ccattaagta
     7981 caggtttaca atccttgccc ttgctgtaga gacgaagtcc aggcttgctg attcgggtaa
     8041 ttttacgaat aacaggtaaa tcgtccacat acttcaacca aattcgcaat tccggaaact
     8101 tacctttttc gtccagttcc cagtttacta tgaaaccttc atctttcaga acctttacta
     8161 tgttaagttt catcttggag gacagcattt ccagactttc ttttttcctc atgtttgcat
     8221 tgcggatgcg agttaataaa tccgcaatag ggtcactcat tgacataaca caatcttttt
     8281 cagtatctta tgttgttttt accaactggc ttttgtcacg cccggcagaa ggcctgcatt
     8341 agcgtttttt ctaaaacaaa tcctgcacat accgaacttt ctcaaatatc cacgcggacg
     8401 cccgcaatat ccacatctgc tgtattggcg gactttaaac ttttgagtac gttcgctttt
     8461 tgcgatcatt gatttttttg ccatttaacc tattttctaa agggaaatcc caagtcctgc
     8521 atcattgcaa ggccctcttc atctgtaagc gcactggtta caatggatat attcatacct
     8581 cggatattat ctacatcatc aatttcaatt tcaggaaaaa tcagttgttc tttaattccc
     8641 atggtataat ttcctgctcc atcaaaggct tttggggaaa tacccctaaa gtcacgaata
     8701 cgaggcattg caatgtgaag caatttatct agaaaagtcc acatttgtac gttacgcaga
     8761 gttaccatgc aaccgatagg catgccttca cgtaacttaa atccagcaat tgaacgtttt
     8821 gccttggtaa ccacaggctt ttgtccggaa atcaaggcta actgcttgct ggctgcctcg
     8881 actgctttgg ggttctgtac agcttcacca agtcctatat tcagggttac tttattgata
     8941 gcaggaattt ggtgcttgtt cttgtattta aacttctcct gaagcaacgg cacaactttc
     9001 gaattatagg tctgtaacaa gtgactctct gacattatcc taatacctcc ccagatttta
     9061 cacagactct atttttaaca ccagcatcac ttgtctgtat ttttacacgc acgcctcttt
     9121 ctagagttgg attgtaaagc tgaacgtttg aataatgaac tggtgcttca attttgatca
     9181 agccaccctt ttgatttgct gcattgggtt ttgtatgacg cgtgaccata tttacacctt
     9241 caatcagtaa gcgttcgcgc ttccaatcta cagtaattac acgtccggta cgccccttat
     9301 cttttccgga aataaccatc acagtgtctt cacgacgaat tgtggccctt ctttttttgc
     9361 gttgcttagc tttcatgaat cagactacta taatacttca ggtgcaagtg acaggattcg
     9421 catgaatttt ccagcgcgca gctctctggc tacagggccg aaaatgcggg tacctaccgg
     9481 ttctcttttt ccgtctatta aaacagcaga atttgaatcg aatttaatat aactgccatc
     9541 tggtcgacgg gactcttttt tggtacgaac tacaacggct ttcacaacat ctccggattt
     9601 tactttggaa tgaggttggc agtccttgat tgatgcaaca attatatcac ctacccgagc
     9661 ataacgtctc ttggatcctc caagaacctt tatacacatc agtctttttg ctcctgagtt
     9721 atcagcaact tcaaggatac tttgtgtttg tatcattttg acggtttaaa taatcggtag
     9781 cgcttacgtc tactcaatgg tctgcactct gcaatttgaa ccaagtcacc taccttgcat
     9841 tcgttctgct catcatgtgc aatatatttt tttgagcgtt tgatggtttt cttgtaaagg
     9901 gggtgtttta tttttctttc aatctttaca acaatggatt tatccatctt gttactgaca
     9961 acaattccac tctgtagctt caatgtactc attttaattt tattttcgct attgtttggc
    10021 cagtttttca gttaacactg tattcaaacg agctatattg cgtcgagctt tcttgatcaa
    10081 attaacatca gccaattgcc gggaaacgtg cccgcagcgt aaccgcagat attcttcttt
    10141 gagatcgtat tttttggctt ccaattcatt gagtggtaat gcccgtaatt cagacgcatt
    10201 catattacag ctgctctttt gataattttt gtatttattg ataatttata agcagccagg
    10261 cgtaatgctt cagttgctac ttcttcagga actccgtcaa tttcataaag cacacgtccc
    10321 ggatgcacag aagctaccca agtgtcgaca gctcccttgc ccttccccat tcttgtttca
    10381 gcaggtcgtt ttgtaaccgg cttgtcagga aacacccgaa tccaagtttt tccacctcgt
    10441 tttaacgtcc gtgtgatcac cctgcgagca gcctcaatct gtcgtgttgt cacgtaacct
    10501 cgtccggttg cctgaagtcc gatggaacca aaactaattt tgtttccagt atgtgcgtcc
    10561 ccccgcattc tgccgcgcat ctgcttgcga aattttgtct tttttggcat taacatagct
    10621 aaaactcctt aactgaacga ccgtcttcca cgtgatttac cataatcttc tccgcggtac
    10681 acccagactt ttacgccgat aataccatat gtcgtatttg cttcagcaac accatagtca
    10741 atatctacac gtaaagtgtg taaacgtacc tgtccttctc gtacccattc tgatctggct
    10801 atctctgctc cattcaaacg ccctcctacc attactttgc agcctgctat attcattcgc
    10861 atcgcagcct gtatggaccg tttgactgct cttctgtatg caattcgccg ttcaagctga
    10921 cttgcgatat tttctgcaat caacgttgca tcaatttccg gtcgtttaat ttcctttata
    10981 ttaatggtga catcgttata attacgcttg ctgagttcct tttttaaacg ctcaacctca
    11041 tcgccctttc gtccaatgat gattcccgga cgtgctgaga aaatatttac tttggttctt
    11101 tttgctgtgc gttcgacttc tatttttgaa attgcggcat gcttcaaacg agccttcaca
    11161 aacttttgaa tatccagatc ctgtctcagc tggcttgcat aatctttttc tgcaaaccac
    11221 tttgagttcc atgttctgga aatacccagt cttagaccta ttggattaac tttttgaccc
    11281 acagtttacc ttgaaaaacg tgaattccct ttgaaataga atagggaatt aaattattta
    11341 actttattct ccaacttcca gtgtaatatg gctggagcgt tttttaattc cagatgcgcg
    11401 gcccatagct ctagggcgga atcgcttgag catccgtgcc tcatcaacgt aagcggtaat
    11461 cacacggaaa ttatccacat cagcttctgg atggtgctgg tccgcatttg cgagtgcaga
    11521 ctgcaagact ttcattatcg gttcagcagc tctgtttcgt atcgtgctta attgtgccat
    11581 tgcccggaca acattttgcc ctctaatcat atcgacaacc agacgggctt ttcttggtgc
    11641 cactcttaca tttcttgcag ttgcttgagc agtcattatc gccttaccct cttatcactt
    11701 tttcctacat gtccccgata tgtccgggtt ggtgcaaatt cacccaattt ataacccacc
    11761 atatcttctg taataaaaac aggaataaat ttgttgccat tatgaacagc gatatttgta
    11821 ccaataaaat ccggtaaaat tacagagcgt cgggaccatg ttttgatcaa acgtcggtcg
    11881 ttgtttcctt tagctgcagc aacttttttt tcaagatgag cgtctacgaa cggtcctttc
    11941 tttagggatc ttggcactgt ttatctcctc ttcaatatat atttgtctgt atctttacgt
    12001 ttcctggtct ttgctccttt tgtgggttta ccccacggag ttactggatg tcgaccaccg
    12061 gaagtttttc cttcgcctcc gccatgtgga tggtcaactg gattcataac tacaccacga
    12121 acagtagggc ggcgacctaa ccagcgattc cgacctgctt tacctttatt aatgttcata
    12181 tgctctgcat taccaactgt tccaacagtt gccatgcatt ctattcttac ttttctgatt
    12241 tcaccggagc gaagtttgag ttgagcataa tcatcaccct ttgccatcaa ttgaattgct
    12301 gctccggcac tgcgtcctaa ttgtgctcct tttccaggac gtaattcaat gcagtgaagc
    12361 atcgttccaa caggaatatt ttttaatgga agagcgttac cggtcttaat atcagcattt
    12421 tctccagaaa aaataacatc tccaactttt aatccgaaag gtgtcaggat gtagcgtttc
    12481 tcaccatctg cataatgcag taaactgatt cgtgcacttc ggttagggtc atattcaacg
    12541 ctggctactt ttgctggaat ctcttgtttg tttctggaaa agtcgattat ccgatatctg
    12601 cgtttgtgtc caccaccgtg atgcctgacc gtaatcctgc cattattgtt tcggcctccc
    12661 ttctttttca aaggggccaa aagacttttt tccggcgtgg atgcagttat ttcttcaaat
    12721 acaaagccac tcattcccct tcttccagga gatgtaggtt tgtggaattt aattcccata
    12781 attcttagca gtcagtttac gcaccttcaa aatattcaat agtatcccct tcctttaggg
    12841 ttactattgc ttttttccag ctggacttaa gcccctgata acgtccgagg cgttttttct
    12901 tgccccgtac attcattgtt cttactttca gcactgaaac cttaaaggat ttttcaatgc
    12961 tttccttaat ttgaatttta tttgcccttt ggtcaacttc aaaagcgaac tgattagctt
    13021 cttcttttag tgacaaagtc ttttctgaca tttttggtgc tctaagcaca tcaaaaatat
    13081 tcatctagcc aacctttcct tgtatgcctc aagagcatct ttgaggaaaa ccacttttgt
    13141 gtatttcatc atctcataaa catttaggct tcgaaaactc agaacatgta cgtctggaag
    13201 atttctcgaa gcaagtctca agttttcaga cacctcgtct gctactatta aaactttttc
    13261 tggaagctca agcttggtca gcattgcctt caggttttta gttttattat cagtcagatt
    13321 gaggctgtca agcaccaaga ttccgtcctg tcgaactttc tctgcaaaca ctgaacgtgt
    13381 tgcaagacgc ttagttttct tgttcagtct aaaagagtgg ctacgcattt gcggcccgaa
    13441 aatcgtgccg ccgtggcgac gtatgggcga ttttgcacta cccgctcggg aactgcctgt
    13501 acctttttgt cgatatagtt tacgggtact gtaagctact tctgctcgat ttttagtaga
    13561 atgtgtcccc tgttgatttg cagccagaaa ctggcgcaca acttctgaaa cagcttttct
    13621 gtgatatggt gtttccagaa ttgtatcgtc aatttctatc ttacctgcgg ctttgttgcc
    13681 tgcatcaacg gtttttaatg ttgccatgat tatacttccc aataaacttt atccttcttg
    13741 ctttatggcg atatcaactc cagcagaaat atccaggcgc atcaaagcat ccattgtctg
    13801 tggtgttgtt tccaaaatat acaggagccg tttgtgggta cgaatctcaa actgttctct
    13861 cgactttttg tcaacatgtg gagaacgcaa aacagtgtag ctgttctttc gagtaggaag
    13921 aggaatgggg cctacaattt cagctccagt tcgcttaacc gtatgaacaa tttctccaat
    13981 tgttttatcg agaagtttct cgtcgtatgc cttgaaacgt actttaattt tttgttccat
    14041 caaaccacct ggtagcttat tcaataactt ccgtgacgac tccggagcca atagtccgtc
    14101 cgccttcccg aattgcaaaa cgtacttctt tttccattgc aatgggacat atcaactcaa
    14161 tagtcatttc cacgttgtca cccggcatta ccatctcaat cccgcttggt aaagtaacga
    14221 caccggttac gtcagttgta cggaaataaa attgtgggcg gtagtttgta aaaaacggag
    14281 tgtgtcttcc gccttcatct ttatctagta cataaacctg gcctttgaac tttgtgtgcg
    14341 gtgtaattga gcccggctta caaatgactt gtcctcgttc aatatcttca cgcttgatac
    14401 ctctcaacaa aattccaacg ttgtctccag cctgtccctg gtcaagtgat ttacggaaca
    14461 tttctacgcc ggtaacagtg gttgtttggg tctcacggat tccagtgatt tcaatgtcct
    14521 caccgacctt cactattccg cgttcaattt gtccggttgc aactgtaccg cgtccggaaa
    14581 tagtaaacac atcttcaacg gacatcagga ggtccttgtc aaggtcacgt acaggttcag
    14641 gaataaaact gtcaattgca tccagcagct ctttaataca cacaaatgca gggtcgcttg
    14701 aatcagttgc tgaactttcc ataaccgcca gagctgaacc tttgataata ggagtgtcat
    14761 ctccggggaa atcatagtaa tcaagcaatt cacggacttc catttccacc agctcaagca
    14821 actcttcatc atcgacttga tcaattttat tcagaaacac cacaaggtgc ggaacattga
    14881 cctggcgggc caacaagata tgttcacggg tttgaggcat cgggccgtca gcagcggata
    14941 caacaattat cgcaccgtcc atctgtgctg caccggtgat catgttttta acataatcgg
    15001 cgtgacccgg acaatcgacg tgtgcgtaat gccgattctt agtttcatat tcaatatgtg
    15061 atgtcgcaat ggtgatccca cgctcttttt cctctggtgc accgtcaatt tcatcaaagc
    15121 cttttgcttc agctaaaccg tccatttgca gtactttggt gattgctgca gttaaagttg
    15181 ttttcccgtg gtccacgtgg ccaattgttc caacattaac atgtggtttt gagcggtcaa
    15241 atttttctct tgccatctcg tctcctgttc ctttttctgt tttgactaat taaaattaac
    15301 gctcagccta agctgaccgg ttctccaatg agaatctttc ttgtcgattc agtcacagac
    15361 gtgtagtgac tgaaatgcat ggtaaatgat gcccgcccct gagtgagcga acgtaatcgg
    15421 gtggaatagc cgaacatttc cgccaaagga gcttcagcct caacaacctg aattcctccc
    15481 tgctcttcca taccccgaat acgtccgtgg cgagcattca ggtctccaat aacttcacct
    15541 gtaaaagata caggcgtaac gacctctatt ttcattgagg gttcaagtaa ggccggtcct
    15601 gccttacgta ccccttcccg aagagcgttt acccccgcaa tagtgaaggc aacttcggtc
    15661 gaagattctt ccagatattc tcctgcaacc aagcaaactt tcaagtcaat taatgaaaat
    15721 ccggcaagga ctccattact cattgcatct tgaatccctt gttccagggc ctctgcaaac
    15781 tgcgttggca attcttcagc cgaaagtttg ttttcaaatt caaaaccccc tcctgcctcc
    15841 agtggttcaa ggcttatcct gcagaaagca aattggtctt ttccgtcaag ttgttgctca
    15901 aatcgtgttt ctgagtctgc agcctgcgta attgtttccc ggtatgctac tctgggcgtt
    15961 ccggctttac agtcgatctg gaactcccgc tggagtctgt gaaccataac ttccaaatga
    16021 agttcgccca ttccggaaat tatgttttgt cctgtttcag catcaatcct gctccggaat
    16081 gatgggtctt cctgcatcag gcgtttcagg gcttcttcaa gtttgtcctg atcctgtttt
    16141 gtttttggct caatagcctg atcaattacc ggttccgaaa actgagttct ttccagaatt
    16201 atcggatgat cctttgggca gagcgtgtcc cccgtgatca gttccatacc gatcactgcc
    16261 ccgatatctc ctgcttttat cacggagata tcttcacgct tgtttgcgtg cagttttact
    16321 agattcccaa tccgttcgtg cttacgttta cgcggattaa atactgtttt cccggtttca
    16381 aatgaacctg aataaacacg aacaaaagtc atctgaccgg caaaaggatc acttgccact
    16441 ttgaatgcaa gtgcacacaa ggggtctgat tcagaagcct ctcggctaac cggttttttc
    16501 gttaccggat gatctccctg taccggcgga atatcaaacg gagacgggag gtaatttact
    16561 acagcatcaa gaagttgctg cactcccttg ttcttaaagg cacttccaca caaaaccggt
    16621 gtgattttgc cgcttaaagt tgctttacgc agaactttga cgatctcttc ctgttctacc
    16681 tgttgctctt caacaatttt ttcgagcaag tcatcatcaa aaagagaaag atgctccagc
    16741 atctgcctgc gccaatcctg cgctgatgct ctcaattcat cggagagttc ggcactttcg
    16801 tattcttcac caaatccttc atctttgaaa cgcagttcct gcatcgaaac caggtcgatc
    16861 actcctgtaa attcctcctc ggcgccaatc ggtatttgta tggctaccgg acttgcagaa
    16921 agctggtctt caattgaaat gacagctttc tcaaaatccg caccaacacg atccatttta
    16981 ttgacaaatg caatacaggg gacgttatag cgttttgcct gtttccaaac tgtttctgtc
    17041 tgaggctgta ctcctgctac tgcgcatagc accattactg acccatccag tactctcaaa
    17101 gagcgctcga cctccgctgt aaaatctaca tgtcccggag tgtcaataat gtttaaaaca
    17161 tattcttttt tatcagtctc ccaggaacaa gtagttgcag ctgaggtaat cgtgatccca
    17221 cgctcctgct cctgttccat ccaatccatt gtagcagagc cgtcattgac ctccccgatc
    17281 ttgtgaatcc gtcccgtgta ataaaggata cgctcggtgg ttgtagtctt ccctgcatca
    17341 atgtgtgcca tgatgccaag gttacgccgt tgttttagag gaactgttct tgccacgtcg
    17401 gtttattgat agaggcactg atttgtttca gtaaaagcca gttcgtttac caacggtaat
    17461 gcgcaaaagc cttgttggcc tcagccatct tatgcgtatc ttccttcttt tttactgcgc
    17521 tgcctctgct gtttgaagca tctgcgaact catctgccag cttcgcacgc attgataagc
    17581 cgctgcgggc atttgcattt tcaattagcc agcgaattgc cagtgactga ctcctgctgt
    17641 gtcggacttc cagcggcacc tgataagttg aaccgcccac acgccgagat tttacttcta
    17701 cagacggcat cacgttttca atggcggctt taaaaatctc caatgatgac atttcagatt
    17761 cacgctctga aattatgtcc agtgcactat agaaaatatt ttctgcaata cttttcttac
    17821 catgcttcat taacgaatta atgaatttac tgactaacag atctttatat tttggatcag
    17881 gtaagattac tctttttgtt gcggctctac gtcgtggcat atagtttttc tactgatcct
    17941 ttttgacgcc gtacttggaa cgggactgct tacgttcatt tactccaact gtatcaagtt
    18001 taccgcgaac aacgtgataa cgaactccgg gaagatcttt tattcgcccg cctcgtatca
    18061 gcacaactga atgttcttga agattgtgtc ctattcctgg aatataactc gtcacctcaa
    18121 taccattggt caaacgaaca cgggcaactt ttcgtaatgc ggagttcggc ttcttgggtg
    18181 ttgaagtgta aacccgcaca cagacgcctc ggcgttgtgg gcatgcttcc agtgcagggg
    18241 tatccttttt ggttaatttg cttttacgtc ccttccggac caactgactt aatgtcggca
    18301 tattttgtaa gttcgacttt gctgcttatc caattattaa taatcaatct gtcaatttaa
    18361 cattgaatta aattttcgtc aacatctttt tcattaattc ttgaaaaaaa caaaaacctt
    18421 acaaaaacct ttttcttgag tgaaaattag acatccatca tacttg
//
LOCUS       DTVD01000227            5117 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5500_length_5117_cov_237.792177, whole genome
            shotgun sequence.
ACCESSION   DTVD01000227 DTVD01000000
VERSION     DTVD01000227.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5500_length_5117_cov_237.792177"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="EYO68_08150"
     CDS             complement(<1..1889)
                     /locus_tag="EYO68_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIC07815.1"
                     /translation="MTTQEKAVPVPPAAPSKPDPIVFLEEDEDFDALYEESLKGFHEE
                     DIVSGTVTEITRDYVTVDIGFKSDCLITINEFQNAEGVLQVELGDSVEVYVEALEADE
                     DGVTYLSKRKAEKVRTWEKIGEVYEEDGIVRGTIVARIKGGLSVDIGVKAFLPGSQVD
                     LRPVRNLDKLLGEDFDFKILKFNQKRGNIVLSRRVLLEIDRDEKRLKTLDILKEGAVI
                     KGYIKNITDYGVFVDLGGVDGLLHITDMTWGRIVHPSEMFEIGDEVDVVVLKYFPEDQ
                     KVSLGLKQKDQNPWELVEEKFAIGTKITGKIVSLTDYGAFIEIEPGVEGLIHVSEMSW
                     TKKVRHPSKIVELGEMVEAIVKDLDVERKRISLSLKEVQANPWKLALDRYPVGCIVKG
                     SIRNVTDFGIFVGLDEGIDGLVHVSDISWKQRQFKPADVASKGDEIEVKVLNIDVEQE
                     RLSLGIKQLTEDPWKNLDENNPLGTDITGTVVNLTDFGIFVEVSDGVEGLVHISEIDD
                     SIQKNKIHEVYPVGTEVKTKVMKIDLEERRLGLSITEILSTPPPVVEAPEEDSKAKAD
                     SAEEEKQSVSAEAEPTQTDKEEAEKESVTTDEIEDKVEAEAETPPVEEKEDAVEAEAA
                     PSVEKT"
     gene            complement(2220..2888)
                     /locus_tag="EYO68_08155"
     CDS             complement(2220..2888)
                     /locus_tag="EYO68_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07816.1"
                     /translation="MNEELYLVAYKDIEQKEIDEALWLKAMSHAGGDKTKAKWAYIEL
                     RVDQLLRDPSLRHSASKKVRKPTHQSGAYMMWFSILLFFTIISAAVVVDVEEMTLVFS
                     NGLYVLDAWSLIFVLPASIFFGISATSWRTYLRCWTYTFGSAKRVTIIDARAVARCLN
                     VMGLVSLKMGVIGTLLIVIFMFHDLDNWKIKVTMAVITLFYGVVFKLIAYVVEQRVLN
                     HYVH"
     gene            complement(3080..3997)
                     /locus_tag="EYO68_08160"
     CDS             complement(3080..3997)
                     /locus_tag="EYO68_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIC07817.1"
                     /translation="MNRPLNGVYTASLTPLTASFEPNLPALVRHAQWLLESGSDGVVL
                     LGSTGEANSLTIEQRLAIIELSAKKLPTDRLMMGTGSCALQDAVHLTKASIDAGVYTV
                     LVLPPFYYKPQSDKSVLRFFSELISIVDDPRLRIIFYNFPKLSGYNFNVKILQELKQR
                     FGDIAAGIKDSSGEWKNMLGIVQNIPDFMVYTGTETLLLDILKKGGAGCITATANLIA
                     PECQRVYQAWRNNKQKVAEQAQINLTDLRKTLENYPLVSELKSLFASETNAEEWENML
                     PPFAPLLDDQVGELKEQIRGLGLDLSQRL"
     gene            complement(4107..>5117)
                     /locus_tag="EYO68_08165"
     CDS             complement(4107..>5117)
                     /locus_tag="EYO68_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HIC07818.1"
                     /translation="EMREAMAQAPVGDDVYGEDPTVNALEERVAEILGKDAAIYMSSG
                     TMTNQVAVRTHTEPGDEILIDKNAHIYFYEAGGTAALSGVICSLISGERGVFGAKEME
                     DVISEKEPHTTIMRPVDLHLPRTKLVCLENTTNRGGGKVWPLEKIAEVEKVARSKNLK
                     MHLDGARIWNAATNLKVQESDIARHFDSVSVCFSKGLGAPVGSALVGSFDFIMEARRF
                     RKQFGGGMRQAGIVAAGALYALENNRKRLNEDHANAKLFARGLEEIKGIQINPEDVET
                     NILIFSLSEMSSHEFAERLSEQDTHMLPWDNTSIRAVTNLMVTEDQILKALEQIRQVV
                     SE"
BASE COUNT         1430 a         1144 c          923 g         1620 t
ORIGIN      
        1 gttttttcca ctgaaggagc agcttctgct tcaactgcat cttccttttc ttctaccgga
       61 ggagtttctg cttctgcctc aactttatct tctatttcat ccgtggttac agactctttt
      121 tctgcttctt ctttatctgt ttgtgtaggt tcagcttcag cactaacgga ttgtttttct
      181 tcctctgcag agtcagcttt tgctttagag tcttcttctg gagcttcaac aactggaggc
      241 ggagtcgaaa gtatttcagt gatactcaac cctaaacgtc tttcttccag atcaatcttc
      301 attacttttg tttttacttc tgtccctaca ggatatactt catggatttt gtttttctga
      361 atggaatcgt ctatttcaga aatatgtacc aaaccttcta ctccatcgga tacctcaaca
      421 aaaatgccaa agtcggttag atttaccact gttccggtta tatctgtgcc aagaggatta
      481 ttttcatcaa gatttttcca tggatcttct gttaactgtt tgatgcccaa cgagagacgc
      541 tcttgttcta catcaatatt aagtactttc acctcaattt catcaccttt actggcgacg
      601 tcagctggtt taaactggcg ttgtttccag gaaatatctg atacatgtac caggccgtca
      661 attccctcat caaggccaac aaatataccg aaatctgtta cattgcgaat agatcctttg
      721 acaatacatc ccacaggata gcggtcaaga gcaagtttcc acggatttgc ctgaacttct
      781 ttaagtgaaa gtgaaattcg tttgcgttcc acgtcaagat ctttgacgat tgcttccacc
      841 atttcaccaa gttcaacaat ttttgaagga tgccgaactt ttttggtcca tgacatttca
      901 gaaacatgga tgagaccttc tactccaggt tcaatttcga taaatgcacc atagtctgtc
      961 agactaacaa ttttaccggt aatttttgtt ccaatggcaa atttttcttc aaccaattcc
     1021 cacggatttt gatctttttg cttgagtcca agtgatactt tctgatcttc aggaaaatac
     1081 ttcaatacta caacatctac ctcgtcacca atttcaaaca tctcagacgg atgaacgata
     1141 cgtccccatg tcatgtctgt gatgtgcagc aaaccatcca ctccgcctaa atccacgaat
     1201 acaccataat cggtgatgtt cttgatatag cccttaataa ctgctccctc tttgaggata
     1261 tccagtgttt taagacgttt ttcatcgcga tctatttcca gcaaaactct acgggaaagg
     1321 acaatgtttc cgcgtttttg gttgaacttc aaaattttaa aatcaaaatc ttctcccagc
     1381 agtttatcaa ggttacgtac gggacgtaaa tcaacctgag atccaggcag gaaagcttta
     1441 actccaatat ctacggaaag acccccctta attcgtgcca caattgttcc gcgtacaatc
     1501 ccgtcctctt cataaacttc tccaattttc tcccaggttc ttaccttttc tgctttgcgt
     1561 ttcgaaagat aagttactcc gtcttcatct gcctccaggg cctcaacata gacttccaca
     1621 gagtctccaa gttctacctg gagtacacct tcggcattct gaaattcatt tatagttatc
     1681 agacagtctg atttgaaacc aatatcaaca gtaacataat cacgggttat ttccgttacc
     1741 gtaccggaaa caatatcttc ctcgtgaaaa cccttcaggc tctcttcata gagtgcatca
     1801 aagtcctcat cttcttctaa aaatacaatt ggatctggtt tggatggagc ggcgggggga
     1861 actgggactg ctttttcttg agtggtcact tttaccttta agttgctgga acagctttta
     1921 cagaaaaagc tggttagggt tgttggcagt actgaaaatt aagagtcagt taaaaacaga
     1981 aaacagctaa ttcaagaatg tttttctgct ttatgaagtt gtcttccaga caggccaata
     2041 atttatacac ttcttctaca tgaagaactg caaatcaaga gagtctatga ttctccggat
     2101 tatctttcta aatgtcaata ttattgtatt aaaaaattga ctggagtcaa tttttcccgg
     2161 tactgtttta gttaaggagg gaaaactgtt gaagaggtca gaagagtgga atttattatt
     2221 taatgtacat aatgattcag cacccgctgt tctacaacat aagcgatgag tttgaaaaca
     2281 acaccataaa ataaggtaat tacggccata gttaccttaa ttttccagtt atccaaatca
     2341 tgaaacatga aaataacaat tagaagcgtt cctataactc ccattttcag ggaaacaaga
     2401 cccataacgt tcaggcagcg tgctacagca cgtgcatcaa tgatcgtaac ccgcttggca
     2461 cttccaaaag tgtaagtcca acaacgcaag tatgtacgcc aggaggttgc cgaaatacca
     2521 aaaaaaatgg atgcaggtaa aacaaaaatc aaggaccaag cgtctaaaac gtacaatcca
     2581 ttggaaaaaa caagggtcat ttcttcaaca tccacaacca cagctgcgga gataattgta
     2641 aagaataaca gaatactaaa ccacatcata taagctcctg actgatgagt tggcttgcgg
     2701 accttcttgc tggcgctgtg ccgaagagaa gggtcccgca acaattgatc gactcgtaat
     2761 tcaatgtagg cccacttggc tttagtttta tcgccaccgg catgagacat tgccttgagc
     2821 cagagggctt cgtcaatttc tttttgctcg atgtccttgt aggccaccag ataaagttct
     2881 tcgttcataa ttctatctat tttgttaggc cgaagcgctt gtcaaatgag agtcgtttat
     2941 cactgacttt tgttacctga aatgctttag gtgttgatca tttttttata cttgcatatt
     3001 tttgaccgtt tttctcaaaa aacttccatg ttctttgaaa aaattaacta tgggacattg
     3061 aaattacata tgacatcaat cacagacgtt gactcaagtc caatccaagc cctctgattt
     3121 gttcttttag ttctcctact tgatcatcta aaagcggggc aaatggcggc agcatatttt
     3181 cccattcttc tgcgttagtt tcagaagcaa agaggctttt gagttcagag acaagcgggt
     3241 aattttctaa agtttttcgt aaatccgtga ggtttatttg ggcttgctct gcgacttttt
     3301 gtttattatt tctccaggct tgataaacac gctgacactc cggtgcaatc aggtttgctg
     3361 tggcggtaat acagcccgct ccgccttttt ttaatatgtc cagcagtaaa gtctcagtac
     3421 ctgtgtacac cataaagtcc ggaatgtttt gtactatgcc caacatattc ttccattctc
     3481 ctgaactgtc cttgatccct gcggctatgt ctccaaatcg ctgctttaat tcctgtaaaa
     3541 ttttgacatt gaaattatag ccgcttagtt ttggaaaatt ataaaaaatg attcgcagtc
     3601 gcggatcatc cacaatggat atcaactcag aaaaaaaacg gagaacactt ttgtcgcttt
     3661 gcggtttgta ataaaaagga gggagcacca aaactgtata aacacctgca tcaatgctgg
     3721 ctttagttaa gtgtacagca tcctgaagag cacaagagcc agtacccatc ataagacgat
     3781 ccgttggtaa tttctttgca gatagttcaa taatggccag acgttgttca atagtcaacg
     3841 aattggcctc gccagtgcta ccaagtaaca ctaccccatc agaccctgat tcgagtaacc
     3901 attgtgcatg acgaactaat gctggtaaat ttggttcaaa tgaggctgtg agaggtgtca
     3961 aggaagctgt atagacaccg tttagagggc gattcatacg caaaactgtt aaaggttgag
     4021 gaaaagatga tatgacaaaa acgattagtt aaactatcaa attttcaaaa tagtatttat
     4081 ttttgtgaaa gatctaaaca cagagcttat tccgatacaa cttgacgaat ttgctctagt
     4141 gccttgagaa tttgatcctc tgtgaccatc aagtttgtga cggctctgat tgaagtgttg
     4201 tcccacggta gcatgtgagt atcctgttca ctcagacgct cggcaaattc atgtgaagac
     4261 atttctgaca aactgaaaat taagatattt gtttctacat cttcaggatt gatttgaatt
     4321 cccttaattt cttcaagtcc cctggcaaat aattttgcat tcgcatggtc ttcattcaat
     4381 ctttttctgt tattttcgag cgcatacaaa gctcctgcag ctacaattcc agcctgtctc
     4441 atcccgccac cgaattgttt cctgaatcgc ctggcctcca ttataaaatc aaagctgccc
     4501 accaaagctg aacccactgg cgccccaagt cccttggaaa aacagacact gactgaatca
     4561 aaatgacgtg caatatcaga ttcttgaact tttaaattag ttgccgcatt ccaaattcgt
     4621 gctccatcaa gatgcatttt taaatttttt gatcgggcaa ctttttccac ttcagctatt
     4681 ttttcaagtg gccaaacttt tccgcctcca cgattggtcg tattttctaa acaaaccagc
     4741 tttgtacgag gtaaatgcaa atccactggt ctcataattg ttgtatgagg ttctttttcg
     4801 gagataacat cttccatttc ttttgctcca aaaactcctc tttctcccga aattaagctg
     4861 caaatcactc ctgagagtgc cgccgttccg ccagcttcat aaaaatatat gtgtgcattc
     4921 ttgtcgatta atatttcatc tccgggttca gtgtgtgttc ttactgccac ttgattggtc
     4981 atggttccgg aagacatata aatagctgca tcttttccga gaatttctgc gacacgttct
     5041 tccagagcat tgacggttgg atcttcacca taaacatcat cacccacagg agcttgtgcc
     5101 attgcttccc tcatttc
//
LOCUS       DTVD01000228           18098 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_553_length_18098_cov_293.666353, whole genome
            shotgun sequence.
ACCESSION   DTVD01000228 DTVD01000000
VERSION     DTVD01000228.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 18098)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18098)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18098
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_18098_cov_293.666353"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="EYO68_08170"
     CDS             complement(<1..776)
                     /locus_tag="EYO68_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIC07819.1"
                     /translation="MSNYKHLFIFLVIAFSFEPAFGVSSFSADEKENILIYEKSSRAV
                     VNISNIAVNYDFFYRAMPAETGSGTGFLIDKSGIIVTNYHVVENASKLVVTLADNSQW
                     PGKLVGADPNNDLAIVRIKAPADSYDILEFSHSNNIVVGQKVLALGNPFGLRQTLTTG
                     IISALGRTIAAKNGRKIEGIIQTDAAINPGNSGGPLLDSEGKVIGINTAIIGSAGSVG
                     IGFAVPSNTALRILPDLLKYGYVRRPWLGIEPIPTVYLRR"
     gene            complement(802..1533)
                     /locus_tag="EYO68_08175"
     CDS             complement(802..1533)
                     /locus_tag="EYO68_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC07820.1"
                     /translation="MELQDKVALVTGAGVRLGQAVAQQLAQLGMRVVIHYHHSEKGAK
                     QTVQGLTGGESRHLILQADLKEVSSIKELVHTAEEKSGPISVLINNAADFFPTPLFST
                     TEEEWDHLLALNLKAPFFLAQTVAEGMKSRGEGKIINMVDSSTERPWVSFLPYCTSKA
                     GLVSLTKGLARALSPEVQVNGIAPGTVLTPPDHAEMNFSSSVEHSLLKRIGSAEDIVQ
                     AVEYLLLSDFVTGTILPVDGGRSVY"
     gene            1567..2601
                     /gene="pdxA"
                     /locus_tag="EYO68_08180"
     CDS             1567..2601
                     /gene="pdxA"
                     /locus_tag="EYO68_08180"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIC07821.1"
                     /translation="MKPEIKIGLTMGDPNGIGAEVLLRALQFMHPFQDWEPLIFGDLK
                     ILTGMNKILDAPFSFKSISEKHSTQTKKVIDSFCLPVIDLSVQKDRKWELGTSSAWGG
                     ESAFQFVHNAIDWANRKKIAAIVTAPLAKETLQAAGHSFPGHTEMLSHYSNGARSVMM
                     LAVDDLRATMVTLHVSLRQALESLTPELILEVMEITVSGLKNMGITDPILGIPGLNPH
                     AGENRLFGDEEDIIIRPAMELARGKGIRCEGPFPPDTVFLEHRKGRFDAVVALYHDQA
                     LIPLKLFGFERAVNVTLGLPIIRTSPDHGTAFELAPQIAANPRSMIEAIKTAVKMANS
                     SHFTTDKVVS"
     gene            complement(2722..4506)
                     /locus_tag="EYO68_08185"
     CDS             complement(2722..4506)
                     /locus_tag="EYO68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIC07822.1"
                     /translation="MNHSPHFQLYSFKTRLLTVLAFFGICFLAVLGRVFFLQILKADY
                     YIQKAKSQHERTVTLEPHRGRILDKNGRILAVSIQLKSLFAKPTQIESPAQAARLLTP
                     ILKIPYHKLLNDLKSKRNFIWIKRKLPPEQAKLVQKLSLNGIGFIEEFRRFYPNRNLA
                     GQLIGYSGIDTQGLEGIESKYEPLLAGKPAVYVVEKEGMFRTVPLSNIPKKIPDQYSI
                     QLTIDSTIQHFAEKALREGVVKSQADRGTVVALHSKTGAILAMASYPEFDPNRYQNYS
                     RAHHLNQAVTLGYEPGSTFKMITIAAGLSEGLISPDQEFFCENGEYKIGINLIRDSSP
                     HGVMTLQQVLKKSSNICAAKIGMSMPPTRFHEYIMRFGFGRRPNSGIAAEATGRVISP
                     KNWQTIDHANISFGQAILVSPLQMVSAANVFANEGEWVPPFIVEYAVDKKGNKLQEIK
                     DVEGKVSHKFGLGVRSPVVEGSVAELVRKYLISVTQKGGTAQRAAILGYQVAGKTGTS
                     QIYDQQLGRYSKTRYIASFVGFAPASDPLVTVLVVVEQPRTSHYGGTIAAPIFKEIVQ
                     RTLLFKDVLPFPEGNSEETTAQVETASR"
     gene            complement(4514..4900)
                     /locus_tag="EYO68_08190"
     CDS             complement(4514..4900)
                     /locus_tag="EYO68_08190"
                     /inference="COORDINATES: protein motif:HMM:PF04999.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07823.1"
                     /translation="MSLSSIVHSARRKPRSLKHQFRWKLVPRFIWQSMQSFRITLLVW
                     GLVTIGCIFYLSQWMNYRQVAHRIDSLETKHSKMFYQLELLEVETSYLTRPQRLEALA
                     GKTMLMASPDTVQYQLSWVDVSSSAE"
     gene            5200..7050
                     /locus_tag="EYO68_08195"
     CDS             5200..7050
                     /locus_tag="EYO68_08195"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00441.22,HMM:PF02771.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC07824.1"
                     /translation="MSSTELTLDQRDIRFAAQEWLKIGQLSESEKFAEFDQDTLDLMV
                     QEGIRFAIDVVSPTRSESDREGCRIEDGRVKVPKCLHEPYQQSYELGWASMGVSQEYG
                     GMGAPATLGLIVNEAMNGANLGLSMFFGLTSGAADLIDTYGSDSLKQKYVAKMYQGKF
                     TGTMCLSEPQAGSDVGAGTTSAESVEDGVYKIKGSKCWISSGDSDLGENVIHAVLARV
                     KGAPDGTKGLSLFVVPHTRVNDDGSLGDWNDVTVVSIEDKLGIHASPTAVLNFGENGD
                     CLGWIIGEEGKGISSMFVMMNGARIFTGLIGLSLAGAAYENARTYAYERVQGTNISKI
                     RDPEAPRVTIVEHPAVRLNLINMKAKVEAMRALLYYTVFQLDLELIAKTDEERQKAQN
                     MVDLLTPMCKGWCTEVGLDVVQVGIQVLGGVGYTKDFPMEQLFRDARIAPIYEGTTDI
                     QALDLVGRKMIQNDGILFQQLMEQFSQSIEKHREHPELSEIFKTFEGHCETLYETSLG
                     SQEVLKTRGMEGVALYATPFLMYISSISAGWLLLQQAVVATEKLGQIKTENGVGDLSD
                     SSFLKENEDALFYANKLKTTRYFVEAIIPQFEALLAGGRKQNFDALEIVF"
     gene            7098..9254
                     /gene="fadB"
                     /locus_tag="EYO68_08200"
     CDS             7098..9254
                     /gene="fadB"
                     /locus_tag="EYO68_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HIC07825.1"
                     /translation="MEYKGQAIQCRTLGNNIVELTFDAQGESVNKFDQNSLSELSEVV
                     KELEADKSVSGLLVSSGKGVFIVGADITEFLKGFQVPQDELVQWVRDAQQVFTDLENL
                     HFPSVCAINGYALGGGFEFGLACTYRVASTKAIVGLPEVKLGLLPGFGGTSRLPRLVG
                     ADNALEWIAGGTEYKPEKALQIGAVDAVVEPEILRDAALDLLTRAIGGELDWQAKRAE
                     KLEALKLNENEAMMTFETARAFIAGKAGPNYPAPLTIVGVMQASARFTLEGALGIEAE
                     GFAELAKSKEATALIGLFLGDQLLKKKAKQSAKQAGPVKRAAVLGAGIMGGGVAYQSS
                     YRGIPIAMKDIAQAQLDLGMSEATKIMQKRVDRGRMEPKKMAQVLTGITPTLSYENLK
                     DADLVVEAVVEKESVKKIVLAETESLLRENAILTSNTSTISITKLAQGLKRPEQFCGM
                     HFFNPVHRMPLVEVIRGEKSSESTIAATVTYAAAMGKSPIVVNDCPGFLINRVLFPYF
                     AGFSGLVNDGVDFERIDKVMEKFGWPMGPAFLLDVVGIDTAFHAGVVMSEGFPDRMSQ
                     DVPTIISKMYEKKWYGQKNENGFYIHSKDKKGRPKKEATPGVADFLQDLGVGSDDSIS
                     DAEIIDRMMLPMLMESSRCLEDNIVETPMEVDMGLIYGLGFPPFRGGIFRWADNVGLN
                     EIIQRAEKHNALGKVYEPTEQIRKKAQEGQLFHPVK"
     gene            9385..10548
                     /gene="fadA"
                     /locus_tag="EYO68_08205"
     CDS             9385..10548
                     /gene="fadA"
                     /locus_tag="EYO68_08205"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HIC07826.1"
                     /translation="MQEAVFIDAVRTPMGRSKGGMFRNIRAENLSAALINELLNRYPE
                     LNPEDVEDVIWGCVNQTKEQGFNIARFASLMTSLPHTCSAQTVNRLCGSSMTAVHTAA
                     MSIMCEQGDVFFCGGVEHMGHVPMDHGLDPNPHISKFSAKASGMMGLTAEMLSIQHKI
                     SREDQDSFALRSHKNASQATKSKRFEKEIVPIEGHDEEGKLMLCTDDEVIRHDASMEA
                     LAALRPVFNPVSGTVTAGNSSAISDGASAVLMMSAQKAKDLGLKPFAKVKAMATAGVD
                     PAIMGYGPVPAVKKALKRGGLVIGDIDLFELNEAFAAQSLPVLRDLKIDSMMDEKVNL
                     NGGAIALGHPLGCSGTRILGTLLHEMREQDSTLGVATMCIGLGQGIATVVERV"
     assembly_gap    10712..10811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10812..11885)
                     /gene="pyk"
                     /locus_tag="EYO68_08210"
     CDS             complement(<10812..11885)
                     /gene="pyk"
                     /locus_tag="EYO68_08210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIC07827.1"
                     /translation="MDDRRIKTKIICTIGPETESFEMLQRMAGAGMNIARLNMSHGDH
                     DWHKHVIKLIKTLNRQVKFPIAILLDTQGPEIRTGHLASDLDLKKGSVISIVVRGESE
                     VEETSIHVDYDDLISSVHAGDKITVDNGLINLEVLEKHERMMRCRVLDGGVLKSKSHV
                     NLPGIRVNLPAITQKDRRDILFGIEHDVDYIALSFVREASDIHQLKELLGDKVGKIKV
                     IAKIEDQEGVSNVNEIIAEVDGVMVARGDLGIEIPIHTVPTVQRKIVRSCSEQGKRVI
                     VATHLLESMISNPIPTRAEVTDVANAVFEEVDAVMLSGETTVGKYPLRCIDQLVNIAE
                     HVEKHPGLRFVDKLNTDSDKQKLS"
     gene            12163..13206
                     /gene="mtnA"
                     /locus_tag="EYO68_08215"
     CDS             12163..13206
                     /gene="mtnA"
                     /locus_tag="EYO68_08215"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIC07828.1"
                     /translation="MTSLPQTIRWNAGELYLLDQTKLPIEVIEEKQESVEQVWDSIKQ
                     LKVRGAPAIGVAGAYGLLVAIREQTALNKKELLQEIEVKAEYLDSARPTAVNLKWALN
                     RMLNSAKTFSGNGSRGLYKHLEEEAIRIHEEDVQLCLKMGINAVLLIKEGMGVLTHCN
                     AGALATTGIGTATAPMYLAHQNGVQFRVYSDETRPLLQGARLTSWELQQSGLDVTLLT
                     DNMAAHIMSQGLIDLVITGTDRVAANGDVANKIGTHGLAILAKHFGIPFYVACPYSTI
                     DLNTAEGSEIVIEERKAEEVTHFGLRRTAPEEMKVCNPAFDVTPNELVTGLITEKGIM
                     RAPFGENLSKEYL"
     gene            13226..>13893
                     /locus_tag="EYO68_08220"
     CDS             13226..>13893
                     /locus_tag="EYO68_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIC07829.1"
                     /translation="MKNLWDPKEASQFEENSLQMRVYSSRLLGNNSDLVLHGGGNTSV
                     KFQETNLFGEEEEILYVKGSGCNLATIEKNGFTPLRLKTLRKLAEVENLSDAEMVRQQ
                     QMAMTNPDAPSPSVEALLHAVIPFKWIDHTHADAVVTITNTPRGKELIQEIYGERLFI
                     IPYVMPGFKLVRKVHELARNVDWNQYEGMVLLNHGIFSFAESALESYTRMIDLVSLAE
                     KYLG"
     assembly_gap    13894..13993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13994..15317
                     /locus_tag="EYO68_08225"
     CDS             <13994..15317
                     /locus_tag="EYO68_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC07830.1"
                     /translation="DSVPGKAFFPEHPTLQTLARIRSKVSEIRGSAMLAQLNYGPEAR
                     GFANLPNVKEIATRGTITSDHLIRTKPVPAVLDPENLEKSLENFASGYKAYFERQTNG
                     QQTCLDCAPRWGVWPGKGTVAFGRNITESGIVSDIVEHTVKAIQHAEAIGGWKPVTEE
                     HLFEAEYWELQQAKLKPRNDVRGVKNNTPEFEGKIALVSGAASGIGLACARELFEQGA
                     VVVGLDLNPDISNILSEQGMLGIECDVTDQKAVSEAVAVTVRKFGGLDVLVLNAGTFP
                     AGQTIEEMDEQTWSKSLAINLTAPQQLLQSCVPFLKEGIDPAVIFMASRNVPAPGPGA
                     SAYSVPKAGQTQMARIAALELGKFGIRVNILHPDCVYDTGLWTPEALERSAKRYGLTV
                     EEYKGRNVLKKDVKTKEVARMVCAMAGSVFAKTTGAQIPIDGGNERVI"
     gene            15354..16235
                     /gene="lsrF"
                     /locus_tag="EYO68_08230"
     CDS             15354..16235
                     /gene="lsrF"
                     /locus_tag="EYO68_08230"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HIC07831.1"
                     /translation="MADLDAVKETKEYYLDIPQKSEAFYLKGSNALGWGMQNRLARIF
                     NPKTGRTVMLAFDHGYFQGATTGLERIDVNIMPLAPYADTLMLTRGILRSVVPPSMTK
                     AIVMRASGGTSMLKELSNEEIAVDIEDSIRMNVAAMAVQVFIGGEHEKQSIINMTKLV
                     DQGTRYGIPTLAVTAVGKEMVRDARYFRLATRICAELGAHYVKTYYVEKDFETVTASC
                     PVPIVIAGGKKLPELDALKMACNAIQQGAAGVDMGRNIFQSDAPVSMIQAVRAVVHDN
                     ESPEKAFELYETLKAEG"
     gene            complement(16281..17336)
                     /locus_tag="EYO68_08235"
     CDS             complement(16281..17336)
                     /locus_tag="EYO68_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIC07832.1"
                     /translation="MHFNYYSLFLIAALTLGLGYFANNVHAEAQVIDRIYLIVNSQML
                     TRSEAQDVAAAIQAQKSSAEKTKKELNEQLLMNLVQEMLLLDRAKALKIEPGVKEIES
                     RLDRLADDQPQLLEVYSEEDLKEQLVRDYKKQHIVNREVDSKIRIESHEIKFFCEQQL
                     RKERKVGLAQILLQGSDEEIQQQVSTIRQAFKSGITFEELSKLHSADPSAQRTGGKLG
                     IFKPEDLLAEIGEATKNLKRGEISNVVQTSIGNHLLYIYEEQFPQDLDCTNLSEDQNN
                     KYSNALYSEKREALLDTYMDELYACAHVEIKDPGTSGLPALSVLPEVEKENVNCQARR
                     VMVLPQKKKKKKKRIKR"
     gene            complement(17326..>18098)
                     /locus_tag="EYO68_08240"
     CDS             complement(17326..>18098)
                     /locus_tag="EYO68_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07833.1"
                     /translation="AAPAAAFFPLTWDDLMFQMEKRMSWKRAKMETVVQVFDPFAKNA
                     DGEFSKNPVELPARSFKQVIHWKDGEILIVETQDEQGQLLHFYYENKDELLSISLNDN
                     RTLETEDILPHQLRFRSRYEDDRSRALEETGIIYKAIAYHISDDNHVFLRIGDFESGH
                     FALLNPKTYDLISIHNRLWLEDENWLELKIVFKNYETYRWQTYAKVTEYFLDNRLFKR
                     TTVSKIRTLSRLPIKGLQEQAWKLRHLQTATLQNNYAL"
BASE COUNT         5079 a         3571 c         4083 g         5165 t
ORIGIN      
        1 atgcggcgca gataaactgt tggaattggc tcaatcccaa gccacggtct tcgtacataa
       61 ccgtatttca ggaggtctgg tagaatccgt aatgcagtat tgctgggcac agcaaaacct
      121 attcccacac ttcctgcaga tccaatgatt gctgtgttaa ttccaataac tttcccctca
      181 gaatccagta aaggacctcc tgagtttccg ggattgattg ccgcatctgt ttgaattatc
      241 ccttcaattt tccttccgtt ttttgctgca atggtgcgcc ccaaggcact gataattcca
      301 gtcgtaagag tttgccgaag accaaaagga tttcccaggg ccaacacttt ttggcctaca
      361 acaatattgt ttgaatggga aaattccagg atgtcgtagc tgtctgcagg tgctttaatc
      421 cgaacaattg ccaaatcgtt attaggatct gcaccaacca gttttcccgg ccactgactg
      481 ttatcagcaa gtgtcactac cagtttgctg gcattttcaa ccacatgata attggtgaca
      541 attattccgg atttgtcaat taaaaatccg gtccctgaac cggtttctgc cggcattgca
      601 cggtagaaaa aatcatagtt gacagcaata ttgctgatgt ttaccactgc tctgctgctt
      661 ttttcataaa tcaggatgtt ttctttttcg tcggcagaaa aactggaaac accgaaggca
      721 ggctcaaaag agaatgcaat aactaaaaat ataaaaaggt gtttgtagtt actcatgaaa
      781 atttattctc actcctgatt attaataaac agaccgtcca ccatcaactg gcaggatcgt
      841 accggtaacg aagtctgata gtagcaaata ttcaacggcc tgtacaatat cttccgccga
      901 gccaatccgt ttgagcagac tgtgctcaac tgaagagcta aagttcattt ccgcatgatc
      961 tggaggagtc aacacggttc ccggagcaat gccgttgacc tgtacttctg gtgacaaagc
     1021 acgtgccagt cctttggtta aagaaacaag tcctgctttt gatgtacaat agggcaaaaa
     1081 actcacccat ggtcgctcag ttgaagaatc gaccatatta atgattttac cttcaccgcg
     1141 tgatttcata ccctcagcta ctgtttgtgc taaaaagaat ggtgccttca gattcagcgc
     1201 taataaatga tcccattctt cttcggttgt agaaaataat ggtgttggga aaaaatctgc
     1261 tgcattattg attaaaactg aaatggggcc ggatttctct tcggcagtat ggactagttc
     1321 tttaattgag gaaacttctt ttaaatctgc ttgcagaata agatgtctcg actctccacc
     1381 tgtaagtcct tgaacggttt gttttgcgcc tttttctgag tggtgatagt gtatgacaac
     1441 acgcatgcca agttgagcca attgttgggc aactgcttgt cccagacgga caccggcacc
     1501 tgttacgagt gctactttat cttgcaattc cataaatata tttttattta tttgtttgaa
     1561 aaaaccatga aaccggaaat taaaatcggc ctgacaatgg gtgatcccaa tggaattgga
     1621 gcggaagtcc tgctccgggc cttacaattt atgcatcctt ttcaggactg ggaaccttta
     1681 atttttggcg atctcaaaat tttaactgga atgaacaaga tcctggacgc acctttttct
     1741 tttaagtcaa tttcagaaaa acacagcact cagacaaaaa aagtcatcga ttctttttgt
     1801 cttccggtaa tagatttatc ggtacaaaaa gatagaaaat gggaactcgg tacttcatct
     1861 gcgtggggtg gagagtcagc attccagttc gttcataacg ccatagactg ggctaatcgt
     1921 aaaaaaattg ccgccatagt tacggctccg cttgctaaag agacacttca ggcagctggt
     1981 cactcttttc cgggtcacac agaaatgttg tcacattaca gtaacggtgc acgttcagtg
     2041 atgatgctgg ccgtggatga tttacgtgcc acaatggtaa cccttcatgt atcacttcgt
     2101 caagctttgg aatcactcac acctgaacta atcctggaag taatggaaat cacagtatcg
     2161 gggctgaaaa atatgggaat tacggatccg atcctcggta tcccgggact caatcctcat
     2221 gctggtgaaa acagattatt tggagacgaa gaagatatca ttattcgtcc tgcaatggaa
     2281 cttgcccgtg gaaaaggaat acgctgtgag ggaccgtttc ctccggatac agtttttttg
     2341 gaacatcgca aaggacgttt tgatgcagta gtagccctgt atcacgatca ggctctcata
     2401 ccgttaaaat tatttggttt tgaacgtgct gtaaacgtaa ctcttggact gccgattatc
     2461 cgtacttctc cggatcacgg aactgctttt gagttagctc cacagattgc tgctaatcca
     2521 cgttcgatga tagaggcaat caagactgca gttaaaatgg cgaattcatc tcattttact
     2581 actgacaagg tagtatcgtg atggagttaa aaagacggac aaactatttt cagtaatgct
     2641 cctgtggcag gttatttaaa aaacttttga aaacacaaaa ttgttcccag tctcaaaacc
     2701 attaacaata catctttaaa atcagcgtga ggccgtttct acctgggcgg tggtttcttc
     2761 tgaatttcct tccggaaatg gtaatacatc tttaaacaaa agagttcgtt gaacaatttc
     2821 tttgaaaata ggtgcagcaa tagttccacc ataatgtgaa gttcgcggtt gttcaacgac
     2881 aacgagtact gttaccaaag ggtcagaagc aggagcgaat cctacaaatg aagctatgta
     2941 gcgtgttttg gaatatcgtc caagctgttg atcatagatt tggctcgtac ctgtttttcc
     3001 tgcaacttgg taacccaaga ttgcagctct ttgagcagta cctccttttt gtgtcacaga
     3061 aattagatac tttctgacca gttccgcaac agagccttcg accacaggac tgcggactcc
     3121 taaaccaaac ttgtgactca ctttaccttc tacatctttt atttcctgta atttatttcc
     3181 ctttttatca acggcatact ccacaatgaa aggtggtacc cattctcctt cgtttgcaaa
     3241 aacatttgct gcactcacca tttgcaaagg tgaaacaaga atagcttgtc caaaagaaat
     3301 atttgcatga tcgattgtct gccaattttt tggagaaata actcttccgg tagcctctgc
     3361 tgcaatgccg gagtttggac gacgaccaaa accgaagcgc atgatgtatt catgaaaacg
     3421 tgtcggtggc atactcatac caatttttgc tgcacatata tttgaagatt tttttaatac
     3481 ctgctgaagt gtcattacac catgcggtga cgagtcacgg atcaagttaa tgcctatctt
     3541 gtattcacca ttttcacaga aaaattcttg atcaggtgat ataagcccct cactcaggcc
     3601 tgcagcaatt gtaatcatct taaaagtgga accgggctca tatcccaaag taacagcttg
     3661 attcagatga tgtgctcggc tgtagttctg ataacggttt ggatcaaact ctggataaga
     3721 tgccatagcc agaatggctc cagttttgga atgaagtgcc acgacagttc cccgatcggc
     3781 ttgtgatttc accactcctt cccgcagggc tttttctgca aaatgctgaa tagtactgtc
     3841 tattgtcagt tgaatagaat attgatctgg tatttttttt ggaatgttgg aaagtggtac
     3901 tgtacggaac ataccttctt tttccacaac atataccgcc ggttttccgg caagtaaagg
     3961 ttcgtatttg ctctcaatac cttccaaacc ctgggtatct attccggagt atcctataag
     4021 ttgtccggca agatttctat taggataaaa tcgacggaac tcctcgataa aaccaattcc
     4081 gttcaaactc agcttttgta ccagtttagc ctgttccgga ggtaatttgc gtttgatcca
     4141 gataaaatta cgtttagatt taagatcatt cagtagttta tggtaaggaa tttttagaat
     4201 gggagtaaga agtctggcag cttgtgcagg tgattctatc tgtgtgggtt ttgcaaaaag
     4261 cgattttagt tgaattgaaa ctgctaatat acgcccgttt ttgtccaata ttctgccacg
     4321 atgtggttcc agtgttaccg tacgttcatg ttgagacttt gctttttgaa tataataatc
     4381 tgcttttaga atctgcaaaa aaaagactct gcccaaaact gcaagaaaac aaataccaaa
     4441 aaatgcaagt actgtgagga gacgtgtttt gaaactgtag agctgaaaat gcggagaatg
     4501 attcatatta gtcttattca gcgctgctgc ttacatcaac ccaggaaagt tgatactgta
     4561 ctgtgtccgg agaggccata agcattgttt ttcccgccag agcttccagt cgctgcggac
     4621 gtgtaagata gctggtttct acttccagca attctaactg gtaaaacatt tttgaatgct
     4681 tagtttccag cgaatcaatg cggtgtgcaa cttgacggta attcatccat tgtgaaagat
     4741 aaaaaataca cccaattgtt accagacccc aaaccagtaa tgttatgcga aaagactgca
     4801 tagactgcca tatgaatcga ggcaccagtt tccagcgaaa ttgatgtttt aaagatctgg
     4861 gttttcgcct ggcggaatgt acaatagaac taagggacat tgaaaagtct ttctaataaa
     4921 aactgaaact acgtttattc cggttttgct atctccttta atttatataa aaaagatcac
     4981 atttataact tttgcacaaa ctgtcccaaa tagtaaaagt atccctaata ctttgatcgt
     5041 caaatactat gcaaataggt ttgtttttcc aaatccaatc tttccggaaa agtctttctt
     5101 ctccacacta attaaggtaa tttaaatgca tgcagtcata agattaattt tttaaactgt
     5161 aaaaccatta attgttatct gcaatatctc taaaataaaa tgagcagtac tgaattaact
     5221 cttgatcagc gtgatattcg tttcgccgca caggaatggc ttaaaattgg tcaactcagc
     5281 gagtcagaaa aatttgctga atttgatcaa gatacgcttg acttgatggt tcaggaaggt
     5341 atccgttttg cgattgatgt ggtttcacca acacgttctg aaagtgatcg tgaagggtgc
     5401 agaatcgaag acggccgcgt aaaagtgcca aaatgcctgc atgaacctta tcaacaatct
     5461 tatgaacttg gatgggcttc gatgggagtg tctcaggaat atggcggaat gggtgctcct
     5521 gcaacactcg gattaattgt caatgaggca atgaacggag caaatcttgg gctgagcatg
     5581 ttttttggac tgacttccgg agccgctgat ctgattgaca cctatggttc tgactctctg
     5641 aaacaaaagt atgtagctaa aatgtatcaa ggtaagttta cggggaccat gtgcttgagt
     5701 gaacctcagg ccggttcgga tgtcggagca ggtacgacca gtgccgaatc agtcgaagac
     5761 ggagtttaca agatcaaagg ctccaagtgc tggatttctt caggtgacag tgatttgggt
     5821 gaaaacgtga ttcacgcagt actggctaga gtcaaaggtg ctccggatgg aacgaaaggt
     5881 ttgagcctgt tcgtagttcc tcacacacgt gtgaatgatg atggttcgct tggagactgg
     5941 aacgatgtca ctgttgtctc cattgaagat aaattgggaa tccatgccag ccccactgca
     6001 gttttgaatt ttggagaaaa cggggattgc ctgggttgga ttattggaga agaaggcaag
     6061 ggaatttcct caatgttcgt gatgatgaat ggggcacgta tatttaccgg tttgataggt
     6121 ttgtctctgg caggtgctgc atatgaaaac gctagaactt acgcatacga gcgtgtgcag
     6181 ggcacaaata tttcaaaaat tcgtgatccg gaagcacctc gtgttacgat tgtcgaacat
     6241 ccggcggtac gactcaattt gatcaatatg aaggccaaag tcgaagctat gcgtgctttg
     6301 ctctattata ctgtttttca attggacttg gaactaattg caaaaacaga tgaagaacgg
     6361 caaaaagcac aaaacatggt ggatttgctg acaccgatgt gcaaaggctg gtgtacagag
     6421 gttggtctgg atgtagttca agtcggaatt caagtacttg gcggtgttgg atacaccaaa
     6481 gattttccta tggaacaact ttttcgcgat gcccgtattg cgcctattta cgaaggaacg
     6541 actgatattc aagccctgga cctggtgggg cggaaaatga tacagaatga cggaattctt
     6601 tttcagcaat tgatggaaca gtttagtcag tcgattgaaa aacacaggga acatccggaa
     6661 cttagtgaaa tattcaaaac atttgagggg cactgtgaaa cactttatga aacatctttg
     6721 ggctcacaag aagtactgaa aacaaggggt atggaaggtg ttgcacttta tgcaactccg
     6781 tttttgatgt atatttcctc aatttcagca ggttggctct tgttgcagca agccgtcgtg
     6841 gccacagaaa aattgggaca aataaaaact gaaaatggag tcggtgacct ttctgattca
     6901 agttttttga aagagaatga agacgcgctt ttttatgcaa acaaacttaa aacaacacgt
     6961 tattttgttg aggctataat tccgcaattt gaagctttgc ttgcaggagg cagaaagcaa
     7021 aacttcgatg cgttggaaat tgttttttga aatgaggcgg acatgtaaaa ccgccctact
     7081 gttttaataa ggaaattatg gaatataaag gccaggctat acagtgccgt actctgggaa
     7141 ataatattgt agagttgaca tttgatgcac agggagaatc ggtcaataaa tttgatcaaa
     7201 actctctttc agaacttagt gaagtagtca aagagttgga agctgataaa tcagtcagtg
     7261 gcctcctggt ctccagcggg aagggtgttt tcattgttgg agccgatatt acagaatttc
     7321 taaaaggttt tcaagttcca caggacgagt tggttcagtg ggttagggat gcccaacagg
     7381 tttttacgga tttggagaac ctgcatttcc caagtgtttg tgccatcaac ggttatgcgc
     7441 tgggaggagg ttttgagttt ggtctggcct gtacatacag ggtggcttca acaaaagcca
     7501 tagttgggct tccggaagtg aagcttggtt tgctgccagg atttggaggc acatctcgtc
     7561 tgccgcgtct agttggtgca gacaacgcac tggaatggat tgccggaggg acagagtata
     7621 agcctgaaaa agcactgcaa ataggtgcag tcgatgctgt tgttgaaccg gaaatattga
     7681 gggacgcagc tttggatctg ctgaccaggg caatcggtgg tgaattggac tggcaggcaa
     7741 aacgtgcgga aaaacttgag gcgttaaaac tgaatgaaaa tgaggcaatg atgacctttg
     7801 aaactgcacg ggctttcatt gccggaaagg ctggaccgaa ttatccggct ccattgacca
     7861 ttgtcggagt tatgcaggct agcgcaaggt tcactttgga aggtgctttg ggaattgagg
     7921 ctgaaggctt tgctgaactt gcaaaatcta aggaagcaac ggctttaatc ggtttgtttt
     7981 taggtgacca actgctcaag aaaaaagcaa aacaatctgc aaagcaagct ggaccggtaa
     8041 aaagggcggc agttctcgga gcaggtatta tgggcggcgg ggtggcttat caatcgtcat
     8101 acagaggcat tccgattgct atgaaagata ttgcgcaagc gcaactcgat ttaggcatga
     8161 gtgaagctac aaaaattatg cagaaacgtg tggatcgtgg ccgcatggaa ccgaaaaaaa
     8221 tggcccaggt tttaaccgga atcacgccga cgctgtctta tgaaaattta aaagacgcgg
     8281 atttggtggt tgaagctgtg gttgaaaaag aaagcgtcaa aaaaattgtc ttggccgaaa
     8341 ccgaatcttt actcagagaa aacgcgattt taacttctaa tacctccacc atttcaatca
     8401 caaaactggc ccaaggtttg aagcgtccgg aacagttttg tggaatgcac tttttcaatc
     8461 ctgtccaccg gatgccgctg gttgaagtaa ttcgtggaga aaagagcagt gagtcgacta
     8521 ttgctgcaac tgtcacttat gctgccgcaa tgggaaaaag tccgattgtg gtcaatgatt
     8581 gtcctggctt tcttattaat agagtgttat ttccatattt tgcaggattc tcaggtttag
     8641 ttaatgacgg agttgatttt gaacgcattg acaaagtgat ggaaaaattt ggctggccga
     8701 tgggacctgc ttttttgctg gatgtggtag gtattgacac cgcttttcat gcaggcgttg
     8761 tgatgtcgga aggattccct gatcgtatga gtcaggatgt accgactatt atttcgaaaa
     8821 tgtatgagaa aaaatggtat ggtcaaaaaa acgaaaatgg tttttatatc cacagcaaag
     8881 acaaaaaggg caggccgaaa aaagaagcaa ccccgggagt agctgatttt ctccaggatt
     8941 tgggcgtagg cagtgatgat agcatcagtg acgccgaaat aatagacaga atgatgcttc
     9001 caatgttgat ggaaagctcc cgttgtctgg aagataatat tgtcgagact ccgatggaag
     9061 ttgacatggg gctgatttac ggcttaggtt ttcctccatt tcgaggcgga atctttcgct
     9121 gggctgacaa tgtgggctta aatgaaataa ttcaacgtgc agaaaagcat aatgcattag
     9181 gcaaagttta tgagccgact gagcaaatca gaaaaaaggc ccaggaagga cagttgtttc
     9241 atcctgttaa gtgagcagtt ataaagctca aaaaatcatt aattaatttt acaggtcaga
     9301 acaaagtagt gttagaatta tacgagcatt tgtttcttat ctagaattat ttgttccatc
     9361 acattttaaa ttgaaaaggt cgttatgcag gaagccgtat ttatagatgc tgttcgtaca
     9421 ccaatgggac gttcaaaagg aggaatgttc cggaatatac gtgctgaaaa tttgtcggca
     9481 gcactgatta atgaattgtt gaatcgttat ccggaactga atccggagga tgtggaagat
     9541 gtaatctggg gctgcgttaa ccagactaaa gaacagggtt ttaatatagc ccgctttgct
     9601 tcgttgatga catcactgcc gcatacttgt tctgctcaga ccgttaatcg tttgtgtgga
     9661 tcgtctatga ctgcagttca tactgcagca atgtcaatta tgtgtgagca gggagatgtg
     9721 tttttctgcg gaggggtgga acacatgggt catgtgccga tggatcatgg tttggatcca
     9781 aatccacaca ttagtaaatt ttctgcgaag gcatccggaa tgatgggttt gacggctgaa
     9841 atgctgtcaa ttcagcataa aatatcccgc gaagatcaag attcatttgc tttgcgctca
     9901 cataaaaatg cgtcacaggc tacaaaatcc aaacgttttg aaaaggaaat tgttccaata
     9961 gaggggcatg atgaagaagg taaactaatg ctctgcacgg atgatgaagt aatccgtcat
    10021 gatgccagta tggaagcact tgcggcatta cgtccggttt ttaatccggt ttcaggaact
    10081 gtcactgcag gcaattcctc tgcaatatct gatggtgctt ctgctgtctt aatgatgtcc
    10141 gcccagaaag caaaggatct tggtttgaaa ccatttgcaa aagtcaaagc tatggctact
    10201 gccggtgtag accctgcgat tatgggttat ggccctgttc cggcagtaaa aaaagcgctg
    10261 aagcggggcg gcctggtaat tggagacatt gatttgtttg aattgaatga agcgtttgca
    10321 gcacagtctt tgcccgtctt acgggatctg aaaatcgaca gtatgatgga tgaaaaagtt
    10381 aatttgaatg gtggagcaat tgcgctgggc catcctctcg gctgttcagg cacccgcatt
    10441 cttggaactc ttctgcacga aatgcgtgaa caggattcga cattgggagt tgcaacaatg
    10501 tgtattggtc tcgggcaagg aattgcgaca gttgtcgagc gggtttaaca attcagtatg
    10561 agaaacattc aattgattac tcgtacagga caagtcatct aaaatttatt ttcttaaggc
    10621 aggtgacgaa tttgaattgc atcgattttt cctgagcctg caagtacatc agaaattact
    10681 accacttttt cttcagactg aaaaccttcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn ncgacaattt ctgtttatca ctgtcagtat taagtttgtc aacaaaacgc
    10861 aaacccggat gtttttccac gtgttcagca atgttaacaa gttggtcgat acaacgcagt
    10921 ggatattttc cgacagtagt ttctcctgat aacatcaccg catccacttc ttcaaaaacc
    10981 gcattggcca catctgtcac ttcagcacgt gtgggtattg gatttgagat cattgattcg
    11041 agtaaatgcg tagccacgat cactctcttc ccctgctcag aacaagatcg tacgattttt
    11101 cgctgtacag ttggtactgt gtggattgga atttcaattc ccaggtcacc gcgggcgacc
    11161 attactccat cgacttcagc aatgatttca ttgacattcg agaccccttc ctgatcttca
    11221 atttttgcaa tgactttgat ttttccaact ttatcgccaa gtagttcttt taactgatga
    11281 atgtctgaag cctcccgaac aaatgaaagg gcgatgtaat caacgtcgtg ttcaatacca
    11341 aataatatat cccggcgatc cttttgagtg atagcaggaa gattaactct aattcccgga
    11401 agattgacat gacttttact cttgagaaca cctccgtcaa gtacacggca acgcatcatt
    11461 cgttcatgtt tttccaatac ttccaagttt atcaaaccat tatcaactgt gattttatcg
    11521 cctgcatgga cagagctgat caaatcgtca tagtcaacat ggatggatgt ttcctcaact
    11581 tcactttctc ctcgaactac aatcgaaatc acagatcctt tttttaaatc caaatcagag
    11641 gcaagatggc cggtcctgat ttccggtccc tgagtatcta acaaaattgc aatgggaaat
    11701 tttacctgac ggttgagggt tttgatgagc ttgattacat gtttatgcca gtcatgatct
    11761 ccatgagaca tattgagtcg agcgatattc attcctgctc cagccattcg ttggagcatt
    11821 tcgaaggatt ccgtttcggg accaattgta caaattattt ttgtttttat tcgtctgtcg
    11881 tccatgaaat atttgaactg attaaaaatt aaaattgaac aaagactctt tatcttttga
    11941 aaatattttt cccaaaccta agtgtattac tatgaaaatt atatactttt gatcaataaa
    12001 caacaagctt aacacccgaa tgattgggtt taactgcaga tcaaaagttt ttctgccgaa
    12061 cgcattattt gttttttgaa ttgagcaata ttggaggttg tgttgttata atcagctgga
    12121 atgactattt atcaacaaca tcaaactaat tgaataacaa ccatgacatc acttccacaa
    12181 accattcgct ggaatgcagg tgaactctat ttacttgatc aaacaaagtt gccaattgag
    12241 gtaattgaag aaaaacagga atcagtggaa caggtttggg actccatcaa gcagctgaaa
    12301 gttcgtggtg caccggctat cggtgtggca ggagcatacg gattacttgt cgcaattcgg
    12361 gaacaaacag cattgaataa aaaagagctt ttgcaggaaa tagaagtcaa agcagagtat
    12421 cttgacagtg cccgtcccac tgcagtcaat ttaaaatggg cgctgaaccg tatgctgaac
    12481 agcgctaaaa ctttttccgg aaatggttca agaggtttat acaagcattt agaagaagag
    12541 gcgattcgca ttcacgaaga ggatgttcaa ctgtgtttga agatgggtat taacgcagtg
    12601 ttactgatca aagaaggcat gggggtttta actcactgta atgccggagc acttgctaca
    12661 actggaattg gaactgccac ggctccaatg tatctggcac atcagaatgg agttcagttt
    12721 cgtgtgtact ctgatgaaac aaggccgctg ctccaaggtg cccgattgac cagttgggaa
    12781 cttcagcaat ccggactcga cgtgactttg ctaacagaca acatggcagc gcatattatg
    12841 tcacagggac tgattgattt ggttattacc ggaactgacc gcgtagcggc taatggtgat
    12901 gtggccaata aaattgggac acatggactg gcgatcctgg caaaacactt cggcattccc
    12961 ttttatgtgg cttgtccgta ttccacgatt gacttaaaca ctgctgaagg cagtgaaatt
    13021 gtgattgaag aaagaaaggc ggaagaggtt acccacttcg gtttgcgaag aactgctccg
    13081 gaagaaatga aagtctgcaa tcctgctttt gatgttactc cgaatgaact ggtaacaggt
    13141 ttgataacag aaaaaggaat tatgcgtgcg ccttttggtg agaatttgag caaagaatat
    13201 ctttaatcag agaaataaaa taccaatgaa aaacttgtgg gatccaaaag aagcttctca
    13261 atttgaagaa aactcactcc aaatgagagt ctattcctca cgtttgctgg ggaacaattc
    13321 cgatctggta ctgcacggtg gcgggaatac ttcggtcaaa ttccaggaaa ccaatttgtt
    13381 tggcgaagaa gaagagattt tgtacgtcaa aggcagcggc tgtaatttgg ctacgattga
    13441 aaagaatggc tttacgccgc ttcgtttgaa aacattacgg aaattggcgg aggtggaaaa
    13501 cctcagcgat gcggaaatgg tacgtcagca gcaaatggcc atgaccaatc ctgatgcacc
    13561 cagcccgtcc gtggaagctc tgctgcatgc agttattcct ttcaagtgga ttgatcatac
    13621 acacgcagat gcagttgtaa ccatcaccaa tactccccgt ggaaaggagt tgattcagga
    13681 aatatacggt gagcgtctgt tcatcattcc ttatgtgatg cccggattta aattggtacg
    13741 caaagtccat gagctggccc ggaatgttga ttggaatcaa tacgaaggta tggtactgct
    13801 aaatcatggt attttttcgt ttgctgaaag tgctcttgaa agttacacgc ggatgattga
    13861 tttagtgtcg ctggcagaaa agtatctggg aaannnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnggactcc gttccgggga aagcgttttt tccggaacat ccgacacttc
    14041 aaactcttgc ccgtattcgc agcaaggttt ctgaaattcg tgggtccgca atgttggctc
    14101 aattgaatta cggtccggaa gcccgcggtt ttgcaaacct gccaaacgtc aaagaaattg
    14161 cgacccgcgg tacaatcact tctgaccact tgatccgcac caaaccggtc ccagctgtcc
    14221 tcgatccgga aaatttggag aaaagccttg aaaattttgc ctccggatac aaagcgtatt
    14281 ttgagcggca gactaacgga caacaaacct gtttggattg tgcaccacgc tggggtgtct
    14341 ggccgggaaa gggtacggtc gcattcggaa gaaatataac agaatccgga attgtttccg
    14401 acattgtaga acatactgta aaagccattc agcacgcaga agcaattgga ggttggaaac
    14461 cggttacgga agaacatttg tttgaggcag aatattggga actgcagcag gcaaaactca
    14521 aaccacggaa cgatgtaagg ggcgtgaaaa ataatactcc ggaatttgaa ggaaaaattg
    14581 ctctcgtttc cggagctgca agcggaattg gattggcttg tgccagagaa cttttcgagc
    14641 aaggcgcggt tgttgtgggt ttggatttaa atccggatat ctcaaacata ttgtctgaac
    14701 agggtatgct gggtattgag tgcgatgtga ccgatcaaaa agcagtcagt gaagcagtgg
    14761 cagtaactgt acgtaaattt ggagggttgg acgttctggt tttgaatgct gggacttttc
    14821 cggcaggaca gacgatagag gaaatggatg agcaaacttg gtccaaaagt ctggcgatca
    14881 atttgacagc tcctcagcaa ttgcttcagt cctgtgtccc ctttttaaaa gaaggaattg
    14941 atccggcagt aatctttatg gcctcccgga atgttcctgc tccaggaccc ggagcttctg
    15001 cttattcggt tccaaaagca ggtcaaactc aaatggccag aattgcggct ttggagctgg
    15061 gtaaattcgg aatccgtgtc aatatccttc atccggattg tgtttatgat acaggacttt
    15121 ggactccgga agccttggag cgctctgcaa aacgctatgg tttaaccgtt gaagaataca
    15181 aaggacgaaa tgttttgaaa aaggatgtta aaacgaaaga agtcgcacgg atggtctgcg
    15241 cgatggccgg ttcggttttt gcgaaaacaa caggtgcgca aattccgatt gacggcggaa
    15301 atgaacgggt gatttgaaac actcaaaaac tctataattt aaaaggaaat taaatggctg
    15361 atttagatgc agttaaagaa acgaaagaat attatctgga tattcctcaa aaatcagaag
    15421 cattttatct gaaaggttcg aatgcacttg gttggggaat gcaaaaccgg ctggcgcgga
    15481 ttttcaatcc aaaaaccgga cggacagtga tgctggcttt tgaccacggt tattttcagg
    15541 gtgcgaccac cgggcttgag cggattgatg ttaacatcat gccgctggct ccgtatgccg
    15601 atacactgat gttgacgcgt ggtattttac gaagcgttgt tccgccttca atgacaaaag
    15661 ccattgtcat gcgtgcttcc ggaggaacca gtatgctcaa ggaattgtcc aatgaagaaa
    15721 ttgctgtgga cattgaagat tcaattcgca tgaatgtggc tgcaatggca gtgcaggttt
    15781 tcattggcgg agaacatgaa aagcaatcta ttatcaacat gaccaaactt gttgatcagg
    15841 gaacgcgtta cggaattcca actttagctg tgactgcagt cggaaaagaa atggtacgtg
    15901 acgcccgtta tttcaggctg gcaacccgga tttgcgctga acttggtgca cactacgtca
    15961 aaacgtatta tgtcgaaaaa gatttcgaaa ccgtaactgc cagttgcccg gtaccgattg
    16021 tgattgccgg aggaaagaaa cttccggaac tggatgcctt aaaaatggcc tgcaacgcta
    16081 ttcagcaggg tgccgcaggt gtggatatgg gccgcaatat ctttcagtct gatgctccgg
    16141 tctcaatgat ccaggctgtt agagccgtgg ttcatgataa tgaatctcct gagaaagcat
    16201 ttgagcttta cgaaacccta aaagctgaag gttgatttat actcaaaata cagactcaaa
    16261 attattggat gctattccaa ttatcttttg atcctctttt ttttcttttt cttcttttgt
    16321 ggaagcacca ttactctccg ggcctggcag ttaacgtttt ctttttcaac ttcaggcaat
    16381 actgaaaggg cgggaagtcc tgaagtaccc ggatccttga tttcaacatg agcacaggca
    16441 taaagctcat ccatataggt atctaaaagg gcttcacgct tttctgaata caaggcattt
    16501 gagtatttgt tgttttgatc ttctgagaga tttgtgcagt ccagatcttg tggaaattgt
    16561 tcttcataaa tgtagagcag atgattccca atactcgttt gtacaacatt actgatttca
    16621 ccgcgcttta aatttttggt ggcctcacct atttctgcca acaaatcctc gggcttaaaa
    16681 attcccagct ttcctccggt acgttgggca ctgggatctg cagagtgaag ctttgacaat
    16741 tcttcaaatg tgattccgct tttgaaagct tgtcgaatag ttgaaacctg ttgctggatt
    16801 tcttcatccg aaccctgcaa aagtatttgc gccaaaccca ctttgcgctc ttttctcagt
    16861 tgttgttcac agaagaactt aatttcatgg gattcaatcc tgatttttga atcgacttca
    16921 cggttaacaa tgtgttgttt tttataatca cgtaccagtt gttcttttaa gtcttcttct
    16981 gaataaacct caagcaattg aggctggtca tctgcaagcc gatccagtcg ggattctatt
    17041 tcttttactc cgggttcaat ttttagcgcc tttgcacggt ccagcagcag catttcctgc
    17101 acgaggttca tcaacagctg ttcattcagt tctttttttg ttttttctgc agaagacttt
    17161 tgagcctgaa ttgcagccgc cacatcctgc gcttcactgc gtgtaagcat ctgactgttg
    17221 acgattaaat atattcgatc aattacctgt gcttctgcgt gaacattgtt tgcgaaatat
    17281 ccaagtccaa gtgtgagggc ggcaatcaga aatagtgagt aataattaaa gtgcatagtt
    17341 gttttgtaaa gttgcagttt gtaagtgacg taatttccag gcttgttctt gaagtccttt
    17401 tatcggcaaa cgggaaagcg tccggatttt actcaccgtt gtccgcttga ataatctgtt
    17461 gtctaaaaaa tattcagtaa cttttgcata tgtctgccag cggtaagttt cataattttt
    17521 gaagacaatt tttaattcca gccagttttc atcttccagc caaagcctgt tatgtattga
    17581 aatcaagtcg tatgtttttg gattaagcag ggcaaagtgt ccggattcga aatctcctat
    17641 ccttagaaat acatgattgt cgtcgctgat gtgataagca atagctttat aaataattcc
    17701 agtttcttct agtgccctgc ttcggtcatc ctcgtatctt gaccgaaaac ggagttggtg
    17761 cggcaaaatg tcttcagtct ctaaagtacg gttgtcattc agagaaatgg acagaagttc
    17821 atctttgttt tcatagtaaa aatgtaataa ctgtccttgt tcgtcctgtg tttcaacaat
    17881 tagaatttct ccatccttcc aatgaatgac ctgtttaaaa ctccttgccg gcagttcaac
    17941 agggtttttt gaaaattcac cgtctgcatt ttttgcaaat gggtcaaaca cctgaacaac
    18001 ggtttccatt tttgcacgtt tccaactcat ccgcttctcc atttggaaca tcaaatcatc
    18061 ccaggttagt ggaaaaaaag cagcggccgg tgcagcaa
//
LOCUS       DTVD01000229            5063 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5588_length_5063_cov_447.482428, whole genome
            shotgun sequence.
ACCESSION   DTVD01000229 DTVD01000000
VERSION     DTVD01000229.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5588_length_5063_cov_447.482428"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="EYO68_08245"
     CDS             <1..1313
                     /locus_tag="EYO68_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail-specific protease"
                     /protein_id="HIC07834.1"
                     /translation="AVLRWEDGYTVVNSIVPGGAAARHGKLKADDRIIAVAQGEEAFE
                     SVIDMRLNNVVQLIRGKRGTKVGLQILRKTKKGFDTLKLVIVRDKIILKDGEARTQIF
                     VHPSPEENKTAQETNRIGVIKLPSFYVDFNDRRKNPKNYKSSSRDVKKHLKEFVRENV
                     DGVILDLRSNGGGGLDEAINMAGLFIGHNPVVIVRQSGGRRVTVHRSRERAVYDGPLL
                     IMLNRYSASASEILAGAMHDYQRAILAGDTTTFGKGTVQNIFQLPEGYGALKVTIAQF
                     YRVSGWSTQNRGVETSLVLPSLYNARDIGESTLDNALPWRSIDQVSYRVSGNLKKVLP
                     KLKQLSEDRIANSEFFQKVKEDVQEYLTTIKPLKYTSILKMQEDDLRRKTQRDQELES
                     ASEKADEDNSDDTEIEEEKKEIQRDEYMKESLEILSDYIKLQKK"
     gene            1321..>1845
                     /gene="hpt"
                     /locus_tag="EYO68_08250"
     CDS             1321..>1845
                     /gene="hpt"
                     /locus_tag="EYO68_08250"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIC07835.1"
                     /translation="MSTDSSLETEIDISKKEILISSSEINQRVAQLGKEISVDYQGRE
                     LHLVGVLNGAFIFLADLVRQINIPCQICFLQASSYKDQKVSSGEVTLMHNLDLSGKDV
                     LVVEDIFDTGLTLKYVMEDLLRQKPNSIEACSLLNKKIPNKAEVTVKYIGFDIEDRFV
                     VGYGLDFAEKYREVP"
     assembly_gap    1846..1945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1957..2310)
                     /locus_tag="EYO68_08255"
     CDS             complement(1957..2310)
                     /locus_tag="EYO68_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIC07836.1"
                     /translation="MNQNTETDHRPWGFYTVLADEPDYKVKRIVVYPGQRLSLQKHQK
                     RAEHWYVVSGEGIVTLDGQRLNKKGGESVNIPCGSAHRIENPGTVDLSFIEIQTGDYF
                     GEDDIERLEDDYGRS"
     gene            2732..3334
                     /locus_tag="EYO68_08260"
     CDS             2732..3334
                     /locus_tag="EYO68_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07837.1"
                     /translation="MNAKLKKSILVICLLCFATQAWAVRAKIRFLFPVSTKSVTDGFE
                     DSEIKTSEFVLAFLMPISRNTQLDLGYSYFNARIEDPADSSEGYKGVFRAHLLELGAG
                     YTGFELTRNISLLFEASVRIPVSGQAEVKGNQDSSEASSISGMGYFFGSGIAYGNMEV
                     LLFYQRRNLSFDDLTVLRNGKRQDVALHSTEYGIGLGYTF"
     gene            3344..4348
                     /locus_tag="EYO68_08265"
     CDS             3344..4348
                     /locus_tag="EYO68_08265"
                     /inference="COORDINATES: protein motif:HMM:PF00389.28"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HIC07838.1"
                     /translation="MFNVIVNKEYELLFERLKAEAPDSFALSLADFSHPDEKLNVLLE
                     KADAIIGQVNPSDSQYETAGRLRIIQTMSAGYDRIDLAKAKHHNVLVASNNGANAVSV
                     AEHVLMQIFVLYRQLLFHHRSVTSGPWKNLKHSNRELSGKTLGIFGLGHVGKALAQRA
                     SALGVKVQYFDVVRQSEAEKEWGLKYLFPEELLSSSDIISYHLPKTSFTHHLINRNSL
                     RKMRPDALLINSSRGYIQDENAIVEALNSGQISGAGLDVFEVEPLPENSPLRKLENVV
                     LTPHSAPDNESLILAVRNALDNLLRVSNGNEPQSIAVDHEEVTRQFLERFPEVKLTAS
                     "
     gene            4443..4616
                     /locus_tag="EYO68_08270"
                     /pseudo
     CDS             4443..4616
                     /locus_tag="EYO68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1496 a          987 c         1147 g         1333 t
ORIGIN      
        1 gtgcggtttt gcgttgggag gacggctaca cggttgtaaa ttcgattgtt cccggtggtg
       61 cggccgcaag gcacggaaaa ctgaaagcag atgatagaat tattgcggtt gctcagggag
      121 aagaagcttt tgaaagcgtc attgatatgc gcctcaacaa cgtggtgcag ctgattcgtg
      181 gaaaacgcgg aaccaaggtt ggactgcaaa ttctgcggaa aacgaaaaaa ggttttgaca
      241 cgcttaaatt agttattgtc agggataaaa taattcttaa agacggagaa gcaagaacgc
      301 agatttttgt gcatccttct ccggaggaaa acaaaactgc acaagaaaca aaccggattg
      361 gtgtgataaa actgccgtca ttttatgttg attttaatga ccgccgcaaa aatccaaaaa
      421 attacaaaag ctcatcaagg gatgtaaaaa agcatcttaa agaatttgta cgggaaaatg
      481 ttgatggagt catcctcgat ttacgcagca acggaggagg tggccttgat gaagcaatca
      541 atatggctgg actctttatc ggtcacaatc cggtggtgat agttcggcag tccggaggaa
      601 gacgggttac agttcaccgc agccgtgagc gggcagttta tgatggacct ctgctgataa
      661 tgctcaatcg ctacagtgct tctgcatccg aaattcttgc aggcgccatg cacgattacc
      721 aacgcgcaat tttagcaggt gacaccacaa cttttggaaa aggaaccgta cagaatatct
      781 ttcagcttcc ggaaggttat ggtgcattga aagttaccat cgctcaattc taccgcgttt
      841 ccggatggtc cacacagaac cgcggtgttg aaaccagtct cgttttaccc tccctctaca
      901 atgcccggga tattggtgaa tccaccttgg acaatgctct gccctggcgc tcaattgatc
      961 aggtttccta tcgggtgagc gggaatttga aaaaggttct tccgaaactg aaacaacttt
     1021 cagaagatag aattgccaat tctgaatttt tccaaaaagt taaagaggat gtacaggaat
     1081 atttgacaac aataaaaccg ctgaaataca cctctatcct gaaaatgcag gaggacgatt
     1141 tgcgtcgtaa aacacagcga gaccaggaac tggaaagtgc gtcagagaaa gctgacgaag
     1201 acaattctga tgatacagaa attgaggaag aaaaaaagga aattcagcgg gatgagtaca
     1261 tgaaagaaag tctggaaatt ttgtcagatt acataaaact gcagaaaaaa tagaaaaagg
     1321 atgagtacgg attcttcact tgagacagaa atagatattt ccaaaaaaga gattttgatt
     1381 tcaagcagcg aaatcaatca gcgtgtggca cagctgggaa aggaaatttc tgtggattat
     1441 cagggacggg aactgcatct ggtgggagtg ctcaatggtg ccttcatttt cctggcagac
     1501 ctggtgcgcc aaattaacat accctgtcag atctgttttt tgcaggcttc cagttacaaa
     1561 gatcagaaag tgagttccgg agaagtgaca ctgatgcaca acctggattt aagcggaaag
     1621 gatgttctgg tggttgagga tatatttgat accggattga ccctgaaata cgttatggaa
     1681 gatctcctgc ggcaaaaacc gaattcgata gaagcatgtt cattgctgaa taaaaaaatt
     1741 ccgaataaag ctgaggtaac agttaaatat atcggctttg atattgaaga tcgttttgtg
     1801 gttggttacg gattagattt tgctgaaaaa tatcgggaag ttccgnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnctgtt gaaaaatcag cttcttccgt aatcatcttc
     1981 caggcgctca atatcgtctt caccaaaata atctccggtt tgtatttcaa taaagctcaa
     2041 atcaacagta ccgggatttt caatacggtg agctgagccg cagggaatat tcactgactc
     2101 gccacctttt ttgttgagtc gttgtccgtc aagtgttacg atgccttctc cggacaccac
     2161 ataccagtgt tctgctcttt tttgatgttt ttgcagactg agtcgctgac cggggtaaac
     2221 cacgattcgc tttactttat aatccggctc gtccgccaga accgtgtaaa agccccacgg
     2281 acgatgatca gtttctgtat tttgattcat gcagatgctt taagatgtgg atgattgttg
     2341 atggtattgc aaaaagtctg aaatctacac agtgatttcg accgaataga gaaatcataa
     2401 ttatattagt gaagtgactt ataaaagatt tctcgcttca ttcgaaatga cactgtttaa
     2461 ggcagaatca ttttaagcaa cctgaaaaaa atcacaagac cagacagtct ttgtgaaaac
     2521 aatctgggcg agaattactt tactcgactg ttacgagttt tatcgtgtca ctccgaacgc
     2581 atttgagaaa tcttaaaata tatcagtaca ttatcaggtc tctcttcgtt cgagatgaca
     2641 tgcctcagtc aataatataa gtttcatacg ataattcaag actcacgagt actttaataa
     2701 aaaaccaaac aggctgaacc caagaagttt tatgaacgca aagctaaaaa aaagtattct
     2761 cgtaatttgt ctgctctgtt ttgcaacaca agcctgggca gtaagagcaa aaatccgctt
     2821 tctttttcct gtcagtacaa aaagcgtgac agatggtttt gaggactcag aaatcaagac
     2881 atcagaattc gttctggctt ttttgatgcc gatttcccgg aacacccagt tggatttagg
     2941 atattcatat tttaatgccc ggatcgaaga tcctgccgat tcgtcggaag gctataaagg
     3001 agttttccgt gcccatttac tggaacttgg agcaggttat acagggttcg agctgacgag
     3061 aaatatatcg ctgctttttg aggcttctgt ccggattcct gtcagcggtc aagccgaagt
     3121 gaaaggaaac caggattcct cagaagcgtc ttccatcagc ggaatgggtt atttttttgg
     3181 atccggcatt gcatacggca acatggaagt gctgttattt tatcagcgca gaaatttgag
     3241 ttttgatgac ctgacagttt tgcgtaacgg taagcgccag gatgtggctc tgcattctac
     3301 agaatacggc attggtctcg gatatacttt ttaatgcttt gttatgttca atgttattgt
     3361 caacaaagaa tatgaacttt tgttcgaaag actcaaagcg gaagctccgg attcttttgc
     3421 actgtcgctg gctgattttt ctcatccgga tgaaaaactg aatgtcctgc ttgaaaaagc
     3481 agatgcaatt attggacagg taaatcccag cgattcgcag tatgaaaccg ccgggaggct
     3541 gcggataatt caaaccatga gtgccggcta cgaccggata gacctggcaa aagcaaaaca
     3601 tcataacgtt ttagttgcca gcaacaacgg cgccaatgcg gtctcggttg cagagcatgt
     3661 tttgatgcaa attttcgtac tctatcggca gttgctgttt catcaccgtt ctgtaacttc
     3721 tggtccatgg aaaaatttaa aacactcaaa cagagaactc tcaggaaaaa ccctgggaat
     3781 ttttggactt gggcacgtgg gaaaagcctt ggcacaaagg gcttctgcac tgggtgtcaa
     3841 ggttcaatat tttgatgtcg tgcgccagtc ggaagcagaa aaagagtggg gcctgaaata
     3901 tctctttccg gaagaattgc tttcaagttc tgatatcatc agttaccacc ttccaaaaac
     3961 cagctttacc caccatttaa taaacaggaa ttcactccgg aaaatgcgtc cggatgcact
     4021 tttgatcaat tcctcgcgcg gttatattca ggacgaaaat gcaattgttg aggccctgaa
     4081 ttcaggacaa atttccggag ccggactgga tgtttttgaa gtggaaccac tgccggaaaa
     4141 ctcgccgctg cgaaaattgg aaaatgtggt gctgactcct cacagtgcgc ctgataatga
     4201 aagccttatc ctggctgtcc ggaatgcact cgataatctc ctcagagttt caaatgggaa
     4261 tgaaccgcaa tctattgcgg ttgaccacga agaagtaacg cgtcagtttt tagagcgctt
     4321 cccggaggtg aaactgactg cttcctgact ctttcgtaat ttgctgaaat caagttggac
     4381 tgtgttttgc agaaggtact taagtagaac tattttttag caaactattc ggaccaaagg
     4441 tcatggaaca gcaggtaact aatccgtatt ttgaactggg tgtaaaaccc ggagtatccc
     4501 caaagcagct gaagcaggct tatcgtcagt ctgtgatgcg ctatcatcct gatacggctg
     4561 caggaaaagg gaatgcgaaa aaatttcggc aggtaacaga agcgtatcag cttctgcaga
     4621 aaatgaatgc ctatgaagct gcttcttcca gtaaaaaaac ggtcagcaga acctctaatt
     4681 tgagacagaa gttttcctca gtttttttta ataagagaag gcaggagaac caaactgaaa
     4741 aaccgtggtg ggataatata gatgttcctc agggaaaaaa tgaaaaaatt aaagtaaacc
     4801 gccagactac caatctctca cttgaagaac tgattaactg cgttgagctt tctgcaaatc
     4861 agtatgtgca tcaggtggca ctggaagcaa ttgctgcaaa aagggatgaa ggcggagtga
     4921 aatatttact tcaccttttg caaaactcag aaaattcaaa ccggaatcag gttatacagg
     4981 cgcttggtca atgcggattt caaggggtaa acaagtatct gctggcatat gtaacagatc
     5041 cggatatcga aatctctacc tct
//
LOCUS       DTVD01000230            4949 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5817_length_4949_cov_313.100327, whole genome
            shotgun sequence.
ACCESSION   DTVD01000230 DTVD01000000
VERSION     DTVD01000230.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_4949_cov_313.100327"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /gene="sucD"
                     /locus_tag="EYO68_08275"
     CDS             complement(<1..798)
                     /gene="sucD"
                     /locus_tag="EYO68_08275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIC07839.1"
                     /translation="MSVLIDNNTKLLVQGITGSQGMLHTRGCRDYGTNVVSGVTPGKG
                     GQDFEGVPIYNTVQDAVKATGANASMILVPPPFAADAIMEAADAEIPLIVAITEGVPV
                     LDMVSAVSFVYQKGSRLIGPNCPGIITPGQIKIGIMPGSIHKTGPVGVVSRSGTLTYE
                     AVGQLTRVGLGQSTCIGIGGDPVNGTNFIDCLKLFQEDPQTEAIVMIGEIGGDAEEQA
                     AAYAKDNVSKPIVSFIAGQTAPPGRRMGHAGAIISGGHGTAAEKMSAL"
     gene            complement(945..1913)
                     /locus_tag="EYO68_08280"
     CDS             complement(945..1913)
                     /locus_tag="EYO68_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HIC07840.1"
                     /translation="MSHSILVYEHGGPEVMKWEEIPPQHPGRGEVLIDQKKVGLNFID
                     VYQRSGSYPLSLPSSIGMEGVGLVDTIGEDVENVKVGDRVGYVMGPPGSYAESRLYPA
                     ERLIKLPDYISDSQAAAILLKGLTVSYLINKTFPVKKGQTVLFHAAAGGVGLIACQWL
                     NKLGVEVIGTVSSDEKAEIAKAHGCHHTINYSLEDFTAGVEEITDGKGVPVVFDGVGS
                     DTFEGSLKCLSPFGLLVSFGASSGPPPELSVAVLAKKALYITRPSLYPHTATAELTAG
                     ISSPLFEAVRDRLSISINQSYPLKDAANAHRALQSRKTTGSTILEI"
     gene            complement(1984..3213)
                     /locus_tag="EYO68_08285"
     CDS             complement(1984..3213)
                     /locus_tag="EYO68_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07841.1"
                     /translation="MTTKPLLQEKSSIRIIFWICIAEILTMQGVFTFSSMLPYFLAEW
                     KLDSIDAGWISGIYYGSYMISVMILVSLTDWIDAKRIYIIGVLITLISCIGFALFVEG
                     YWSAMLFRALGGIGLAGTYMPGLKALTDRLSGTSRVRATSFYTSSFGIGSALSFLIGG
                     SILKWFPWQIAFWIAGLCALLALLIVSKVLENRPNRVGSRSGLRSLDFRPVIRNSNAM
                     GWIACYTTHNYELFAFRSWIVVYLTFALTGVTGGFLIQPSNIIFFGVLLGMPSSVIGN
                     ELAMRYGRIRVVVSIMCLSAVLAIVVGNSIGLSAPLLIALVLVYGCFVTGDSASITTG
                     AIESASEENRGVTMAVHSSIGFIGSFLGPIGFGLVLNTFGGHNSSEAWFWAFASTGFV
                     LLLGPILLLILRTVKKT"
     gene            complement(3230..4717)
                     /locus_tag="EYO68_08290"
     CDS             complement(3230..4717)
                     /locus_tag="EYO68_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07842.1"
                     /translation="MVDNFLAGSAALFGDPFTIGIFIFGVIGGMLFGAIPGVSMLTLA
                     AILLPFTASLEPAQGVMLFAVIYCTGTYGGAITAILFNIPGAPENAPTAFDGYPMTRK
                     GQSGKAVGAAVLCSAIGGVSSALIMMAATEPLANWAISNIGPPEIFALVFFGLAVASS
                     VGGRTMWKGWMSVLLGLLIATIGQDPVGGINRYNFGISDLAAGIAFVPAIMGFFAVSE
                     IFVQAEKKAVGMYKAPKLSLDFPTILELWSHKIAVLRSILVGFFCGILPGIGATLAAF
                     MGYGEAVRWSKNKDSFGKGNIEGVISSETANNAATGAAMIPLLALGLPGGAMTALMVG
                     VFQMHDLEPGPMVFYNSPELIWIVFAAMFYANVSILFIGLIETKTILHLLKIPFQFLA
                     PMILLLASIGSYIGRGLVLDVMIMFCTGIMGFLLRRSGYSIPGIVLGIILGKIGEQNF
                     AQGMQMVHYDVLEYLSRPICLLLIIAGFLTLFTGIYKALSYSFKS"
     gene            complement(4725..>4949)
                     /locus_tag="EYO68_08295"
     CDS             complement(4725..>4949)
                     /locus_tag="EYO68_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIC07843.1"
                     /translation="YSATPVFIALTIMLLGEKRIKWVLGITLFIYLVLILLFMVILNA
                     PLPQGNISPFYDFSAFMLTLNTKLHQSLNY"
BASE COUNT         1594 a         1126 c          933 g         1296 t
ORIGIN      
        1 caaagcactc attttttcag cagccgtacc atgtccacct gaaataatgg caccggcatg
       61 tcccatgcga cggccggggg gggctgtttg acctgcaatg aaacttacaa tgggtttgga
      121 aacattatct tttgcatatg cagcagcctg ttcttctgca tctccgccaa tttctccaat
      181 catgactatg gcttcagttt gcggatcttc ttgaaacagt ttgaggcagt caatgaaatt
      241 tgttccattc acaggatctc cgccaatacc gatacaagtc gattggccca aaccaacacg
      301 ggtgagttgt ccaactgctt cataggtaag cgttccggaa cgtgaaacta cacccaccgg
      361 tccggtttta tgaatcgatc cgggcatgat gcctatttta atttgtcccg gagttataat
      421 acccggacaa ttcggaccaa tcaaacggga tcctttttgg tatacaaaag aaactgcact
      481 caccatatcc aaaactggaa ctccctcggt aatggcaact attaaaggaa tttctgcatc
      541 tgctgcttcc atgattgcat ctgcagcaaa tgggggcgga acaagaatca tcgaagcatt
      601 tgcacctgta gctttaactg cgtcctgcac cgtgttataa atcggtacac cttcaaaatc
      661 ctgtccacct ttacctggag ttactccaga tactacgtta gttccgtaat ctctgcagcc
      721 tcgagtatga agcatacctt gtgaaccagt gattccctga acaagtaact tagtattatt
      781 atcgataaga acagacatga aattttgaat caatttggag aaaaatacgt tttgtatacc
      841 agtatagacg agctcattct atgaagcaag aaatcagaca ggaactgcgg atattcttac
      901 tactattgta aattatattc ccctatgttt tattttattt atatctaaat ttccaaaatt
      961 gtagatccag ttgttttgcg actttgcaaa gcacggtgag catttgccgc atccttcaga
     1021 ggatatgatt gattaataga tattgataga cgatctctta ctgcttcgaa caggggagag
     1081 ctaattcccg cggtaagctc tgctgttgct gtgtgtggat aaagactagg cctggtaata
     1141 taaagagctt tttttgccaa cactgctact gataactcag gaggaggtcc tgaagaagca
     1201 ccgaaagaca ccaaaaggcc aaaaggggaa agacacttta aagatccttc aaatgtatct
     1261 gaaccaacac cgtcaaaaac aactggaact cctttcccgt ctgttatctc ttcaacgccc
     1321 gcagtgaaat cctcaagaga gtaatttata gtgtgatggc atccgtgagc cttagctatc
     1381 tcagcttttt catctgagct tacggtacca ataacttcaa caccaagttt atttagccac
     1441 tggcaagcaa taagtcctac tcctcctgca gcggcatgaa acaatactgt ttgacctttc
     1501 ttaactggaa aggttttatt aattagatat gatacagtca aacctttcaa tagtattgct
     1561 gctgcttgag agtcagaaat ataatcaggt agcttaatta acctttctgc agggtataaa
     1621 cgactctctg cataagaacc aggcggcccc ataacatagc caactcgatc accaacttta
     1681 acattttcta catcttctcc aattgtgtct actaaaccaa ctccttccat tcctatgcta
     1741 gaaggcaggc ttaacgggta gcttccagat ctttgataaa catcaatgaa attcaagcca
     1801 actttctttt gatcaattaa aacttcacct cttccaggat gctgaggtgg aatttcttcc
     1861 cacttcatga cctctgggcc accatgttca taaacaagaa ttgaatgaga cattgatttt
     1921 tctctataat ttagattttt tcttcaactt cctttggatg ggcatcatat ccaccagaaa
     1981 taattaagtc tttttcacgg tcctgagaat taataacagg atggggccta acagtagaac
     2041 aaatccggtt gacgcaaatg cccaaaacca tgcctcggag ctgttatgac ctccaaaagt
     2101 attgaggact aatccaaaac caataggccc caggaaagaa ccgatgaagc ctatgcttga
     2161 atggacggcc attgtaactc cacgattttc ctccgaagca ctttcaattg caccagtggt
     2221 gattgaagca gaatctcctg taacaaaaca tccataaaca aggacaagtg caatcaacaa
     2281 aggtgctgaa agaccaatac tgtttccaac cacaatggct aaaactgctg agagacacat
     2341 gatacttact acaacacgta ttctgccata acgcattgcc agttcattgc cgatgacgct
     2401 ggacggcatt ccaagaagga ccccaaagaa aattatgttt gaaggttgaa ttaagaaacc
     2461 accagttaca ccagtcaaag caaatgtcag gtaaacaaca atccatgatc ggaatgcaaa
     2521 cagttcataa ttatgggttg tatagcaggc gatccaaccc attgcatttg agtttcttat
     2581 tacaggcctg aagtccagtg aacgaagacc ggaacgagac ccaacccggt ttggacgatt
     2641 ttccaatact ttagatacaa tcagcagtgc cagcaaagca cataagccgg caatccagaa
     2701 agcaatttgc caggggaacc atttgagaat agagcctcca attaaaaatg aaagtgcact
     2761 accgatacca aatgaggagg tataaaaaga tgtggcacga accctagatg tacccgagag
     2821 acggtctgtc agagctttca gcccgggcat gtaagttcct gccagaccta ttcctcccag
     2881 ggctctgaat aacattgcgc tccagtaacc ctcaacaaac aatgcgaatc ctatgcaact
     2941 gataagagta atcaaaacac cgatgatgta tattcttttt gcatcaatcc aatcggtaag
     3001 gctgaccaga atcatgacag atatcatgta tgatccatag tatatcccgc tgatccagcc
     3061 cgcgtcaata ctgtccaact tccattcggc taaaaaataa ggaagcatgg atgaaaatgt
     3121 aaagactccc tgcattgtca ggatttctgc aatgcaaatc cagaaaatga tccgtatcga
     3181 ggatttctct tgaagtaatg gcttggttgt catgttttgg acaacaattt taggacttga
     3241 atgaatatga caaggctttg tatatacctg taaataatgt caaaaaaccg gcaattatca
     3301 ataacaggca gattggacgc gataaatatt ccagcacatc ataatggacc atctgcattc
     3361 cctgggcaaa attttgttca ccgattttac caagaataat acctaacaca attccaggga
     3421 tagaataacc tgagcgtcta agcaaaaaac ccattattcc agtacaaaac attatcatta
     3481 catcaagcac cagaccgcgt ccaatatatg agccaatact tgccagcagc aaaatcatcg
     3541 gtgccaaaaa ttgaaaaggt atttttagta gatgtaaaat cgtcttagtt tcaattaaac
     3601 ctatgaagag aattgataca tttgcataaa acattgcagc aaaaacgatc catatcaact
     3661 caggtgaatt ataaaaaacc attggacctg gttccaggtc gtgcatctga aagaccccta
     3721 ccattaatgc tgtcatagca ccgcctggaa gtccgagtgc cagcaacggg atcattgcgg
     3781 ccccagtagc agcattatta gcagtttcag aagatatcac cccttctata ttcccctttc
     3841 caaatgaatc tttattttta gaccagcgta ctgcctcacc atagcccata aatgccgcaa
     3901 gggttgcacc gattccagga agaattccgc aaaaaaaacc aaccaggatg gatcttaaca
     3961 cagcaatttt atgagaccat agttctaaaa tagtagggaa atcaagacta agtttagggg
     4021 ctttatacat gccaactgct tttttttctg cttgaacgaa aatttcagaa actgcaaaaa
     4081 aacccataat agcaggaaca aatgctatgc ccgcagcaag atcagaaata ccgaaattgt
     4141 atcgatttat acctccaacc ggatcctggc caatcgtagc aattagtaaa ccgagtaaca
     4201 cagacatcca acccttccac atggtccttc cacctactga ggaagcaaca gcaagaccga
     4261 aaaataccaa tgcaaatatt tctggaggcc ctatattgga gattgcccag ttcgccagag
     4321 gttctgttgc cgccatcatt atcaacgcag atgagacacc accgattgca gaacacaaaa
     4381 ctgctgctcc aactgctttt ccagattgac ctttgcgggt cataggatac ccgtcgaatg
     4441 ctgttggagc attttcagga gcaccgggta tattaaacag aattgccgta atagctccac
     4501 cgtaagtgcc tgtacaatat attaccgcaa aaagcatcac accttgagct ggctcaagac
     4561 tagctgtgaa tggcaacaat atagctgcaa gagttaacat actaacgccg ggaatggcac
     4621 caaacagcat tccacctata acaccaaaga taaatattcc aatagtgaaa ggatctccaa
     4681 aaagagctgc actaccagca agaaaattat caaccatctg taaattaata attaagtgat
     4741 tggtgtagtt tggtatttag agtcaacatg aacgcactga aatcatagaa tggggatata
     4801 ttaccctgag ggagtggagc attgaggatt accataaaca ataatatcag aactaaatat
     4861 atgaataatg taattcctaa aacccatttt atccgttttt cacctaatag cataattgtg
     4921 agagcgatga aaacaggtgt cgccgaata
//
LOCUS       DTVD01000231            4929 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5847_length_4929_cov_332.359869, whole genome
            shotgun sequence.
ACCESSION   DTVD01000231 DTVD01000000
VERSION     DTVD01000231.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_4929_cov_332.359869"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="EYO68_08300"
     CDS             complement(<1..1541)
                     /locus_tag="EYO68_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIC07844.1"
                     /translation="MQKNTVSIDTSVIPAKIEFAPEFNVVVSFIDRHLQEGRKDKVII
                     RETGGEEITYGQLAERVNQSGNVLKNLGLNSGDRLLIMVKDSAEFFYLFWGAIKAGII
                     PVPLNTLLRAKDYAFMIEDSGCAAVIYSPEFRIEVEPALELLSAIPEIILLTEGESEC
                     FLDQTQSADSKLEAAPANATDDCFWLYSSGTTGRPKGAVHSHRDMVVTSQHYGKDTLG
                     VREDDICFSAAKLFFAYGMGNGMTFPLWSGASCVLFPARPTPASTFDVIERFKPTLYF
                     GVPTLYAAQLQSLESAGNETSVSPDLSSLRMSVSAGEALPANIFRRWKEQTGTVILDG
                     IGSTEALHIFISNRADDIKAGSSGRMVPGYAAKIVDETGNEVQQGEEGRLLIQGDSTC
                     KYYWNNSEKTTATIVDGWLNTGDTYHQDDDGYFIYGGRSDDMMKVGGIWCSPFEIEAK
                     LVEHPKVLEAAVVGRKDESGLIKPEAHVVLKDLTDASEETSGELLQHCKTGLAPYKYP
                     RWFQFP"
     gene            complement(1721..3154)
                     /gene="boxB"
                     /locus_tag="EYO68_08305"
     CDS             complement(1721..3154)
                     /gene="boxB"
                     /locus_tag="EYO68_08305"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HIC07845.1"
                     /translation="MKINYSEKIPNNVNLSSDRRLQRALERWQPGYLKWWDELGPEKS
                     TGHEVYLRTAVSVEKEGWAHFDYVKMPDYRWGIFLTPSDSDRTINFGIHKGQPVWQEV
                     PGEYRSELRRLIVTQGDTEPASVEQQRLLGKTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLQGYFGRDGREEAEAMLERHSGDPDKPRILEAFNEETPDWLSYFMFTYFTDRDGNFQ
                     LGSLAESGFDPLSRTCRFMLTEEAHHMFVGETGVGRIVQRACDLMKDHKTENTGATDG
                     VRQYGGIDLETIQKYLNFHFSVSTDLFGQELSTNAATYYNTGIKGRFGETKINDDHLL
                     HDSSYSILELNSEKIVNTEVPALNAVNERLRDDYIKDCDLGVRRWNKIIEDAGIDFRL
                     KLPHRAFHRGIGQFSEIQTDPEGEIITQSEWDRRHSEWMPSQQDQAYVQSLMQPVTEP
                     GKIAGWIAPPRGKINRHPFEYEFVRFN"
     gene            complement(3308..>4929)
                     /locus_tag="EYO68_08310"
     CDS             complement(3308..>4929)
                     /locus_tag="EYO68_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HIC07846.1"
                     /translation="YQHWTIDFDCPIATLKLKVNEDGGLHSGYKLKLNSYDLGVDIEL
                     HDAVQRLRFEHPEIKAVVLTSAMDKVFCAGANIKMLGLSTHAHKVNFCKFTNETRNSI
                     EDASRFSGQTYITAINGTAAGGGYELALASEYIMLVDDGSSTVSLPEVPLLAVLPGTG
                     GLTRIVDKRKVRRDHADYFCTITEGIRGKRAENWKLIDELVPRSKFEQKVEERAIEFA
                     NKSSRPTDAKGIALNPLNRKIEENRITYDHVSVEYERQSGIVNLTIHAPDGDLPTDLE
                     SIHAAGANYWPLAMARELDDAIMHLSFNELELGTWVLRTQGEADQITIIDQTLIRHQE
                     HWLVREIILNIKRVFKRLDLASRSLVTLIEPGSCFAGTLLELVFAADRSYMLDGTFEG
                     GNEVPAMLRSTEMNFGPLPMCNELTRLQTRFLNDPENVENVRAEIGKDLDAVFADELG
                     LVTFIPDDIDWEDEVRIAVEERASFSPDALTGMEASLRFAGPETLETKIFGRLTAWQN
                     WIFQRPNAVGEQGALKLYGTGKQAGFDKKRV"
BASE COUNT         1295 a         1190 c         1031 g         1413 t
ORIGIN      
        1 gggaattgaa accagcgcgg atatttgtaa ggcgcgagtc ctgttttaca gtgctgcagc
       61 agttctccgg acgtttcttc agaagcatcc gttagatctt tgagtaccac atgtgcctca
      121 ggtttgatca atccggactc gtctttccgg ccaacaacag cggcttccag tacttttgga
      181 tgttccacca gtttagcttc aatttcaaat ggtgaacacc atattccacc gactttcatc
      241 atatcgtcac tgcgtccgcc gtagatgaaa taaccgtcat cgtcctgatg ataggtatct
      301 ccggtattga gccagccgtc cacaattgtt gccgtcgtct tttccgaatt gttccaataa
      361 tatttacagg tagaatcacc ctgaatcaat aaacgcccct cctctccctg ttgaacttca
      421 ttaccggtct catcaacaat tttagcagca tatcccggaa ccatgcgtcc actgcttccg
      481 gcttttatgt cgtctgcacg gttggaaata aaaatgtgca gtgcttctgt ggaaccgatg
      541 ccgtccaaaa tcactgttcc tgtttgctct ttccagcgac ggaaaatgtt tgccggcaag
      601 gcttcaccgg ctgaaactga catacgcaaa gatgacaagt caggacttac tgaagtttcg
      661 ttgccggcag attctagtga ctgcaattgt gcggcgtaaa gcgtaggaac gccaaaataa
      721 agtgtcggtt tgaaacgttc aatcacatca aaagtgcttg ccggagtggg gcgtgctggg
      781 aacaaaacac agcttgctcc agaccacaaa gggaaggtca taccgtttcc cattccgtaa
      841 gcaaaaaaga gttttgcggc agaaaaacaa atatcgtctt cacgtactcc gagtgtgtct
      901 ttgccgtaat gttgactggt gacaaccatg tcacggtgac tgtgaaccgc gccttttggc
      961 cttccggtgg ttccggaaga gtagagccag aaacagtcat cggttgcgtt cgccggagcg
     1021 gcttcgagtt ttgagtcagc ggactgtgtt tgatccagaa aacattcgga ttcgccttcg
     1081 gtgagcaaaa taatttccgg aattgctgaa agcagttcca aggccggttc gacttcaatc
     1141 cggaattctg gagaatagat aactgcggcg catccggaat cctcaatcat gaaagcataa
     1201 tctttggccc gcaaaagcgt attgagcgga accggaatta tacctgcttt gattgcaccc
     1261 caaaacaaat agaaaaactc cgcggagtcc tttaccatta tcaacagccg gtctcctgaa
     1321 ttcagtccaa gatttttaag tacatttccg gactgattta ctctttctgc taattgtccg
     1381 tatgtgattt cttcacctcc ggtttcacgg atgatgactt tatctttccg gccttcctgc
     1441 aaatgacggt cgatgaacga aaccaccaca ttgaattccg gagcaaattc gatttttgcc
     1501 ggaataactg atgtgtcgat ggaaacagta tttttttgca ttttatctct tttgtttcaa
     1561 gccacagatt tacacagatt tgaactggtt tattttagcc gctaaaaacg ctaacaaaca
     1621 ctaaggacat tatcttcctt cgaactctta gcggctcaaa taaaagaaca actcagtttt
     1681 atccgcgttg atccgtggta atatttgcga gccgataaaa tcaattgaaa cgcacaaact
     1741 catattcaaa aggatggcga ttgatttttc ctctgggagg agcaatccag ccggctattt
     1801 ttcccggttc tgtaaccggc tgcattaagg attggacata ggcctggtct tgttgtgagg
     1861 gcatccattc gctatggcga cgatcccact cggattgtgt gatgatttca ccttccggat
     1921 cagtttgaat ttctgaaaat tgtccaatac cacgatgaaa tgcgcggtgc ggcagtttca
     1981 agcgaaaatc aatacctgca tcctcaataa tcttgttcca gcgtcgtacg cccaaatcac
     2041 agtctttgat gtagtcatca cgcaagcgct cgttgaccgc atttagagca ggcacctctg
     2101 tattgacaat tttttcacta ttcagttcca aaatggaata acttgaatcg tgaagcaaat
     2161 ggtcatcatt aatttttgtt tctccgaaac gtcctttgat accggtattg taataagtag
     2221 ccgcgtttgt ggagagttcc tggccaaata aatcggtaga aacactaaaa tgaaaattaa
     2281 ggtatttctg tatggtttcc aaatcaatac caccgtactg tcgcactccg tcagttgcac
     2341 cggtattttc ggttttgtga tctttcatca aatcacaggc tctttgaaca atacgcccta
     2401 cgccggtttc tccgacgaac atgtgatgcg cctcctctgt cagcataaaa cgacaagtac
     2461 gggacaatgg atcaaatccg gattcggcca gcgaacccag ttggaaatta ccatcgcgat
     2521 cggtaaaata tgtgaacatg aaatatgaaa gccagtccgg agtttcttcg ttgaaggctt
     2581 ccagaatccg gggcttgtcc ggatctcctg aatgccgctc cagcatggct tctgcttctt
     2641 cacgtccatc acgcccaaaa tagccctgta acagatacac catggcccac aaatgtctgc
     2701 cttcctcaac attgacttgg aacaaattac gcaaatcata aagtgaagga catgtttttc
     2761 ccaagagtct ttgctgttct acagaggcag gttccgtatc accctgagta acaatcagcc
     2821 gacgcaattc ggaacggtat tcgcccggga cttcctgcca gaccggctgt cctttgtgaa
     2881 ttccgaaatt tatggtccga tcagaatcag aaggcgtgag gaaaatgccc cagcgataat
     2941 ccggcatttt tacataatcg aaatgcgccc agccttcctt ttctacacta actgcagttc
     3001 tgagatagac ttcgtggcca gtactttttt ctggaccaag ttcatcccac catttcaaat
     3061 atcccggctg ccagcgttcc agtgcccgtt gaagacgtcg gtcactgctt aagttgacat
     3121 tgtttggaat tttttcgctg taatttattt tcatagaaac ctttgtgttt tttgttatcc
     3181 tgtaagagaa ctcaacagtc acgagtttta tcgtttcatc cctaatgtat gtgggggata
     3241 ctataagata tcagcacttt atcaggtcta ccccttcggt cgagatgaaa tatagaatgt
     3301 cctattatca aacccgtttt ttatcgaaac cggcctgttt cccggtacca tagagtttaa
     3361 gcgcgccctg ctctccaact gcgtttggac gctgaaaaat ccagttctgc cacgcagtca
     3421 accgaccgaa aattttcgtt tccaaagttt ccggaccagc aaagcgcaaa gatgcttcca
     3481 ttccggtgag tgcatccggt gaaaaactgg cgcgttcttc aactgcaatc cggacttcat
     3541 cttcccagtc aatgtcatca ggaataaaag tcaccagacc caactcatct gcaaaaactg
     3601 catccagatc cttgccgatt tctgcccgga cattttccac attttccgga tcattaagaa
     3661 aacgggtctg caaacgtgtc aattcgttgc acattggcaa tggaccaaaa ttcatttcag
     3721 ttgaacgaag catagccggc acttcattgc ctccttcaaa agtaccgtcc agcatgtaac
     3781 tccggtctgc ggcaaatacc aattcgagca aagtgcccgc aaaacaactt ccaggttcaa
     3841 tcagggtgac aagactgcgt gatgccagat caagacgttt gaaaacccgt ttgatattca
     3901 aaattatttc acgtaccagc caatgctctt gatgtcggat caaagtttga tcgataatag
     3961 tgatttggtc tgcttcgcct tgtgtacgca aaacccaggt gcctagttcc aattcgttaa
     4021 aagataaatg cataattgca tcgtcgagtt cacgtgccat tgctaaaggc cagtagtttg
     4081 ccccggctgc atgaatgctt tccaaatcag tcggcaaatc tccatcggga gcgtgaatcg
     4141 tgagatttac gattccggat tgccgctcgt attcgacact cacatgatca taagtaattc
     4201 tattttcttc aattttgcgg tttagcggat tgagagcaat gccttttgcg tcagttggac
     4261 gtgatgattt gtttgcaaac tcaatggcac gttcctctac tttttgctca aatttggagc
     4321 gcggtacaag ttcgtctatc aatttccagt tttctgctcg tttgccgcgt attccctctg
     4381 tgatggtgca aaaatagtcg gcatgatcgc ggcgtacttt gcgtttatcg acaatcctgg
     4441 tcaaaccacc agtccctgga agtacagcta aaagtggaac ttccggtaaa gagacagtac
     4501 tggatccgtc atcaaccagc atgatatatt cactcgccag tgctaattcg tatcctcctc
     4561 cggcagcagt tccgttgatg gcagtgatat atgtttgtcc tgaaaaccgg cttgcgtcct
     4621 caattgagtt tctcgtttcg tttgtaaatt tacaaaaatt cactttatgt gcatgagtag
     4681 aaagtccgag catttttata tttgctcctg cacaaaaaac tttatccatt gctgaagtaa
     4741 gaacaacagc ttttatttcc ggatgttcga agcgtaaacg ctgcactgca tcatgcagtt
     4801 cgatatccac acccaagtca taagagttaa gttttaattt gtatccggaa tgcagccctc
     4861 catcttcgtt aactttcaac tttaatgttg caataggaca gtcaaaatct atagtccagt
     4921 gttgatact
//
LOCUS       DTVD01000232           17529 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_590_length_17529_cov_366.457079, whole genome
            shotgun sequence.
ACCESSION   DTVD01000232 DTVD01000000
VERSION     DTVD01000232.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17529)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17529)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17529
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_590_length_17529_cov_366.457079"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            96..305
                     /locus_tag="EYO68_08315"
     CDS             96..305
                     /locus_tag="EYO68_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07847.1"
                     /translation="MSLISLEDVRPAVLLLTLAPDSVFDETDRLLGMAVTMLESVVVV
                     VLLLLLSSFLAQAANIIAMLIIIHR"
     gene            610..1176
                     /gene="amrA"
                     /locus_tag="EYO68_08320"
     CDS             610..1176
                     /gene="amrA"
                     /locus_tag="EYO68_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIC07848.1"
                     /translation="MKSHHSAENRLKLLRVARESLEHFLNNGQRTEFTTETPELQTKR
                     AVFVTLRKRDSGDLRGCIGQSEPRYPLIEAVSRTAISAAVDDSRFPQVKLNELANLLI
                     EINVLTPMFVITPDNVVVGKHGLLLSKGSCRGLFLPEVAVSRGWDRLTFLDELCRKAD
                     LPRGSWRDADAELQAFESESWEEIENAL"
     gene            1166..2323
                     /locus_tag="EYO68_08325"
     CDS             1166..2323
                     /locus_tag="EYO68_08325"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIC07849.1"
                     /translation="MLFDFIFRQLRPLIFSGDAETAHERMLTIVESISKIPGLIPFLR
                     RQFLEEHSVLRTQLFGRSLQNPIGLAAGFDKDGRIHPALFALGFGFVEIGTVTPYPQA
                     GNPRPRLFRLPEDEGLINRLGFNNLGAQEMAELLVSARKKIMPEDPDIIDRKSDFPAN
                     IAYGMLGINIGKNKDTILEKATDDYVSCLNTLHPFADYFTLNISSPNTEDLRKLQEKD
                     ALRVLLDSVCTHRDNLDQNHIRETPLLVKLSPDLDETALENSIRVIKEFSIQGVIVAN
                     TTVKRPELKSKHRFENGGLSGKPLQKRSTEMIRNVFQKLGTEMPIIGVGGIFSGADAY
                     DKIRAGASAVQIYTALIYEGPGQVRKVKRELAKLLNEDGFTSVSDAVGVDS"
     gene            complement(2334..3719)
                     /locus_tag="EYO68_08330"
     CDS             complement(2334..3719)
                     /locus_tag="EYO68_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HIC07850.1"
                     /translation="MDTSWEIAVIGSGPAGFYATGELFRQQSWEIKVDMFDRLPTPFG
                     LVRGGVAPDHQKIKSVTKIYNRIAENPNFRFFGNVKFGSDLYQPDLLKHYDAVIYSVG
                     SSSDRALGIPGEDLEGSHSATEFVGWYNGHPDFREYKFDLSVKNAFVIGMGNVALDVA
                     RILAKTPEELAATDITDYALEALRKSQIENIWLVGRRGPLQAAFTPVEVREFLELDDA
                     DVMLEGGSLELDEESQKILETEASKDTKKNIEILTQISLRKLSGKKKRVHFLFLASPL
                     EISGDGKVEKISMVRNQLVKQDDGSLRPQATENKIEEDAGLIFRSIGYLGKPLADLPF
                     DQKSGTIPNESGQVKDPENGNSLRNREYVAGWIKRGPSGVIGTNKQDAVETVHRMLET
                     FLAEKMEAGQNVVLPDIVSLLESRKVDHVSFADWKLLDAHETEAGEAQGRPRLKLTSI
                     TEMLEIIRQKR"
     gene            complement(3883..4845)
                     /gene="hemC"
                     /locus_tag="EYO68_08335"
     CDS             complement(3883..4845)
                     /gene="hemC"
                     /locus_tag="EYO68_08335"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIC07851.1"
                     /translation="MSNAFSTSDNKQTNLIIATRGSSLALWQAEWVKQRILDEYPEIS
                     VELLVVKTTGDKIQDRPLSEIGGKGLFVKELEYALLEGRADLAVHSMKDVTSFLPQGL
                     EISVIAEREDPGDAWICPKFGSIENFPDGAVVGTSSLRRASQLQHHRPDLSVMSLRGN
                     VETRLRKLDQGEVDAVILAVSGLKRIKLEKRITEILPIEWMLPAIGQGAIGIETRAGD
                     EATLSRIQHLHDPLTWDCLLAERSLLAELEGNCQLPLAGYCILNGEELFLRAFIGDSE
                     GKIQLRYEASAPRQNALQLGKDVAQWLLKNGGEEILLRAVAAEK"
     gene            complement(4849..6162)
                     /locus_tag="EYO68_08340"
     CDS             complement(4849..6162)
                     /locus_tag="EYO68_08340"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIC07852.1"
                     /translation="MRFTVIGWNFRKTPVEIRDSLALTPKEQVELGLQLRTRFNLGGV
                     VILSTCNRTELYLVNAEQQLDQIIEMLESYWNLYELRERVYTIQNLDGVRHLFRVASS
                     LDSMVLGEPQILGQLKDAFQSFNEADLTGKLLHPLFTRAFSTAKRVRTETSVASNAVS
                     VSYAAVELAKHIFEDLSKQTVMVIGAGEMAELAVRHFMRNGISELFVTNRTFLSAAKL
                     AEKFQGLAVPFEHLERHLHQADIILSSTGARDFIITKEMVKNCLRRRRGRSMFFIDIA
                     VPRDIDPEINELNGTFCYDIDDLQTLVSQNQVERKKQSIKAEEIIENELLKLELWFKS
                     LSAVPTIRSLRKAFHSIAEEEMVKSFKNLKNIPESERKKVEQLVHRLIHRLLHDPSRN
                     LKKIAHDEDAHLYLEYVSKIFDLSPTPVKLEKLPEKHHRLKIVKS"
     gene            6315..6881
                     /gene="pgsA"
                     /locus_tag="EYO68_08345"
     CDS             6315..6881
                     /gene="pgsA"
                     /locus_tag="EYO68_08345"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIC07853.1"
                     /translation="MVAFFSKWLTANHLTTLRVALVPFIYLGMVFGKDDQTILFSTWL
                     VFLFACLTDYWDGVLARHQGQTTKLGKLLDPVADKILIGTLLVLLVGMGRAPALLAVI
                     LISREFIISGLRSIAAAEGVVISASSGGKLKTIFQMFAVGFLIIHYPTFSIPCHEVGI
                     ILLWISTAVSLWSGFNYVVAYFRASPGK"
     gene            7216..7737
                     /gene="hslV"
                     /locus_tag="EYO68_08350"
     CDS             7216..7737
                     /gene="hslV"
                     /locus_tag="EYO68_08350"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIC07854.1"
                     /translation="MTTILCVRKKNRVVMAGDGQVSMQNTIMKPSARKLRNTFDGKVI
                     AGFAGSTADAFTLFEKFDEKLEQYNGKLLRSAVELAKEWRTDKMLRNLEALLAVSSAE
                     ASLIISGNGDVIEPDDGLIGIGSGGMYALAAARALIDTNLSPRKIAEKSMKIASEICI
                     YTNNQITYEELEF"
     gene            7742..9097
                     /gene="hslU"
                     /locus_tag="EYO68_08355"
     CDS             7742..9097
                     /gene="hslU"
                     /locus_tag="EYO68_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIC07855.1"
                     /translation="MDSAPELNMTPQETVDALDRYIIGQTDAKKAVAVALRNRWRRLQ
                     LDEDLKDEILPKNIIMIGATGIGKTEIARRISKLVNAPFVKVEASKFTEVGYVGRDVE
                     SMIRDLTDYSVNMVRQEQMERKQEAAKEMVEERLLDLLLPPLESEKDASEGKASTNPT
                     REKFRKKLRSGVLDERMVELDVSERPSGAVMEFMPVSMNDNMDMNIRDMLSNMMPKQS
                     KRRRVNIEDARKILQQEEVSKLIDMDEVIREALTRVEQSGIVFLDEIDKVASRRSHSH
                     GPDVSREGVQRDLLPIVEGSTVNTKHGPVQTDHILFIAAGAFHLTKPSDMIPELQGRF
                     PIRVELQSLSKDDFVKILTEPKNALIVQYTALMKTEGVELEFTKAAIDTIAEIACQIN
                     EDSENIGARRLHTVMEQLLEEVSFTAPDLNGQKISVTPEYIQSRLSNLLKNQDLSRYI
                     L"
     gene            9225..10049
                     /locus_tag="EYO68_08360"
     CDS             9225..10049
                     /locus_tag="EYO68_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIC07856.1"
                     /translation="MPKVGLRRLLDGLGEILDSPTEDVDKRSKYNFSVSAATQPAVKI
                     KHENADSKIFPPSTGITDLQEKLSAAKQQTVLEAFKQLIPEPEAEIKVSSLSELADKY
                     KSCQRCSLGKTRTNLVFGNGSINSRVIFLGEGPGADEDAQGQPFVGRAGRLLTKMINT
                     IGIERDDVYITNIVKCRPPENRNPTPAEISCCLPIIKQQLELLNPKLIVTLGNIPTKS
                     LIPDLPGITKVRGRIMQYENWTVLPTFHPSYLLRNRSAMPLAWEDFKKIPELAFHS"
     gene            10108..11382
                     /locus_tag="EYO68_08365"
     CDS             10108..11382
                     /locus_tag="EYO68_08365"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIC07857.1"
                     /translation="MIDLQPVKGTRDFFPDEMRLRNWLFEVWRNVSVQAGFEEYDTCV
                     LEHEELYIRKAGDEISKQLYSFEDKGGRRLSLRPEMTPSLARLVLQHKKSLSFPIKWF
                     SMPQCFRYERMTKGRRREHFQWNADIIGQAEIVAEAEILMLLISACELMGLSAKEIRV
                     FINDRRILNSILSQISVPQEMHSEVLVIMDKRDKISIEALSKLLQDIGMSTTQVDQLN
                     EYLLKSDLIELKKDLNDTQGIDELQNLMDMMETAGFSDYLQFDISIVRGLSYYTGAVF
                     EVNSPAKQHRAICGGGRYDSLLSTYGGEIVPAVGFGFGDVVVLDVLKDLERFPELPRK
                     LDYTIIPFAREQVGTALNIAAILRQQGSTVDCNFSMKKMKKTLQQAGESGAAKAILLF
                     PDELSENKVVIRDLRLHEQKPIKITDLLSSGE"
     gene            11388..11912
                     /gene="lspA"
                     /locus_tag="EYO68_08370"
     CDS             11388..11912
                     /gene="lspA"
                     /locus_tag="EYO68_08370"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIC07858.1"
                     /translation="MAISESVFSTSFRTNWIIMIIFLGLDLSSKIWIRLNVPLYETTE
                     LLANILDLTHVQNRGVSFSFLADFSDSYRVPLLVGVSLLAVAAMLYYQTRYWSELDFF
                     TRSGLVLVLPGATGNLIDRAVYGYVTDFLHFHWYETSFFVNNLADCFISLGVIFFIVP
                     LLFSKNGSTSSNSA"
     gene            11884..12420
                     /locus_tag="EYO68_08375"
     CDS             11884..12420
                     /locus_tag="EYO68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIC07859.1"
                     /translation="MEVLHQTQLNSFLFSKIKFVLIFSALFLSSNFIVSGIFAKEEIR
                     YVKGLGAVLREQPSILSKKLTKLSRGTKVLALEAKGIWHKVIAGEIQGWLLRGQITNI
                     PVKIKKLVLGQKIRLTSSSGRRVRLRTFSAVVGVRGLVDSKGEKISPYQTDYVALEWL
                     EKQPTDEENSVRFLTLSE"
     gene            12428..13267
                     /locus_tag="EYO68_08380"
     CDS             12428..13267
                     /locus_tag="EYO68_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M48 Ste24p"
                     /protein_id="HIC07860.1"
                     /translation="MNFFRNHLWFYIFIISFVFASFGNAQEFRLRAHHVYWKNQEANT
                     EREIDFGRSVSAKILGKYPILRNEQKVRYISQIGTGISAQLGRPELRYYFGILDTDEV
                     NAFAAPGGYIFVTKGALKLMRNEAQLAGVLAHELAHIDQKHIVRKLKLQSTDRSVTSG
                     IAQVLGGASLSAKIALERLNDLAFKMLMEEGLSKEDEADADQKALEMLISTGYDLQSY
                     LDYLLSLKPYLEQGQAKVLSKTHPTIEFRYNNLREFIIKNNLEKFQGKNNEIRFKTNF
                     AKL"
     gene            13230..14468
                     /locus_tag="EYO68_08385"
     CDS             13230..14468
                     /locus_tag="EYO68_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HIC07861.1"
                     /translation="MKYALRQISLSFSIFFISCFVATQLYADVDHYKNMLVGERAATM
                     GGTYVAISDDSTGCYYNPAGIAYAVGDSLSGSGNVLHKMKTVYSETIGTEDWVRESET
                     LVPNYFGVLKKYKSYSFCFSYVVPEAFIEHQDLVFNDPLSTVKIYYQSLHSEDITYLM
                     GPSGALQFGENFSLGLTLYYHYRSFMRQYHYFLKSTDGGYQLYYESNKQREDGLMPKI
                     GVQWSPWSLLTVGMALDQTFLFNATFQGNASIHNTDNSTGTASYTSLMTRTISEEKRK
                     FPLHLAFGAAYFPTPSLLYTVDLDYYKAQQKGRADVINYSGGTEYYFDPTNAVRFGLF
                     TNYTNLPQTDSTTIAPYEYIDIYGASFGYTSYSSSSSLTLGAIYTSGSGKAWLYSGST
                     ETRNMKRDSLTLLFSASSSL"
     gene            complement(14493..15893)
                     /gene="pyk"
                     /locus_tag="EYO68_08390"
     CDS             complement(14493..15893)
                     /gene="pyk"
                     /locus_tag="EYO68_08390"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIC07862.1"
                     /translation="MLSTKIICTIGPASDSPKKLAELAEAGMNIARLNFSHGTHHSHK
                     KIIEQIRKLNKDRQYPIAIMLDTQGPEIRTGDKVITLISGKRVCVTIPPNEESEGTLF
                     VNYVDLKDDLKEGSCISLDGGLISLKVCGKNKKGLDCEVIDGGVVKAHRHVNLPGAIV
                     KLPGITETDKQDILFGIEQKVDVIALSFVRSSETIREVRASLGVHAQQIKLIAKIENQ
                     EGVERLEEIVQEADGVMVARGDLGIEVELEEVPQIQRRIAYLCAKYGKRLIVATQMLE
                     SMIENPVPTRAEVTDVANAVFEQTDAIMLSGETSVGKYPVRSVETLVRIARRTENYPG
                     VNYTEKLIKKSKGQHLAEAAIQIAAKLKIRAIVVITRNGRGAEYLSNLRPREIGLYSF
                     TNNSKVFSSMTLYRSVYPFLMEFRKNPEDTIQKALGILQEKEGFAAEEQVVVLANILT
                     GEGYCTSLQIRTIPAV"
     gene            complement(15938..17410)
                     /locus_tag="EYO68_08395"
     CDS             complement(15938..17410)
                     /locus_tag="EYO68_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIC07863.1"
                     /translation="MEKYDLCVIGGGPSGYAAAMRAVDFDKSVLLVERDRIGGAGIYD
                     GALSSKTFWEISKEFASFSKKMSHYGLEEPVVQFHNVLNEVNDAVSERSNQLEDHLRL
                     VIQQRPEGFRYIKGSAHLISSSEIELETDSGTEKVNAENIIIATGSRPRKIPSITIDE
                     DIIMTSDGISSLEEFPESLVILGAGVIGCEFATIFSNFGKTKVHIISKEDRILPFEDP
                     DLAKVIEANLEANGVLIHHESKLDKMEITNGGVEYVLEHPNGEKEVFHAEKALVSVGR
                     VPNIENTGLHEIGMELSESGHIIDNDTQTSISNIYAAGDITADIALVNVGELEGRHAV
                     EKIYGKPKRSVIYENISTIMFLNPEVAGVGMNEQQAQKAELDYRVASYDFRCIPRAIA
                     MRNTQGFFKILVTDDDQMKVLGLRAVGEHASSAIQAVALLIATNKGIEELSELIHPHP
                     SIIEGIQECIRMLLGKSIYKPYIFQEYLQYKRFRDGQYID"
BASE COUNT         5047 a         3746 c         3576 g         5160 t
ORIGIN      
        1 ttttgggaat ggccttatga cactgattga tgtcatcaga atttaagact gcctccaagc
       61 gggcaacctt gtccttatgc tttttgggat cactggtgtc actaatctca cttgaagatg
      121 tacgtcccgc agtcctactg ctgacgcttg caccggattc tgtatttgat gaaacagaca
      181 gattactggg catggctgtc accatgcttg agtcagtggt ggtggtggta ctactgctgc
      241 tgctgtcatc ttttttagca caagcagcaa acatcatagc catgctgata attatacata
      301 gatgagtgaa tctcattttt cctccttagt ttacattaaa ccaatgcttc gttctccatc
      361 atcagccaaa tcaaatcagc ataacgatga tgtactcttt taaacatact ctgatgaaaa
      421 gaaaaaactg cttcgttgtt caaacagagt tgacaactta cctcggtaaa cacttatttt
      481 gaaagatata attattcaga tataaatgtc aagcgtaaaa tgtaaattga agtttttttt
      541 aatcctcaat ttcatgctac actttagcat ggattcatta ccagttaact ctctgtaaac
      601 cgtgaacata tgaaatctca tcactccgcg gaaaacagac tcaaactatt gcgggttgcg
      661 cgggaatcgc tggaacattt tcttaataat ggacaacgaa ctgaatttac aactgaaact
      721 cctgaattgc aaacaaagcg ggcagtattt gtgacgctga ggaaaagaga ttccggagat
      781 ttacgcggat gtatcgggca gtcagaaccg cgctaccctt taattgaagc tgtatccagg
      841 acggcaattt ccgctgctgt ggatgattca cgttttcctc aggtgaaact gaatgagtta
      901 gcgaatttgc tgattgaaat aaatgtactc acgccaatgt tcgtcataac tcctgataat
      961 gtggtggttg gaaaacacgg attgctgctc agtaaaggat catgcagagg gttattttta
     1021 ccggaagtag cagtttcacg aggctgggac cgcctcactt ttctggacga gctgtgccgt
     1081 aaagcagatt taccaagagg cagttggagg gatgctgacg ctgaacttca ggcctttgaa
     1141 tcagaatctt gggaagaaat tgaaaatgct ctttgatttt atttttcgtc agctgcgccc
     1201 gcttattttt tccggagacg ccgaaaccgc acatgagcgg atgctgacga ttgttgaaag
     1261 catttcaaaa attcccgggc tgataccgtt tttacgacgg caattcctag aggaacattc
     1321 cgttttgcgg acccaacttt ttggtagatc tctgcaaaac ccaattggac ttgcagcagg
     1381 atttgacaaa gacggacgga ttcatcccgc attattcgca ctcggttttg gtttcgtgga
     1441 aattggaacg gttactccat atcctcaagc cggtaatcca cgcccacgtc ttttccgcct
     1501 ccctgaagat gagggattga taaaccgtct tggtttcaac aatcttgggg cacaggaaat
     1561 ggcagaatta cttgtttccg cacgaaagaa aatcatgccg gaagatcctg atattattga
     1621 tcgaaaaagt gactttccgg caaatattgc ttacggaatg ctcggcataa atattgggaa
     1681 aaataaagat accattttgg aaaaagccac tgatgattac gtttcttgtc ttaacaccct
     1741 ccatccattt gcagattatt tcaccctcaa tatcagttct ccaaacacag aggatttgcg
     1801 aaaattacag gaaaaagacg ctctgcgtgt tttacttgat tctgtctgca cgcaccgtga
     1861 taacctggac cagaatcata tccgggaaac acctctgctt gttaaactgt ctccggacct
     1921 ggatgaaact gcattggaaa acagtattcg tgtgattaag gagttttcaa ttcaaggcgt
     1981 gattgtcgcc aacacgactg tgaaacgtcc ggaactaaaa agcaaacatc gcttcgaaaa
     2041 cggaggtctg agcggaaaac cgctgcagaa acgcagcacg gaaatgataa gaaatgtgtt
     2101 tcagaaatta ggaacagaaa tgccgattat aggtgtgggt gggattttca gtggagcaga
     2161 tgcatatgac aaaatcagag ccggcgcatc cgcagtgcaa atttacacgg cactgattta
     2221 cgaaggtccc ggacaagtcc ggaaagtaaa acgggaactg gcgaaattat tgaacgagga
     2281 tggattcact tctgtttcag atgcggtagg agtggacagt tgaaactgcc gcgttatctc
     2341 ttttgacgaa ttatttccag catttcagta attgaagtca gcttcagcct gggacgcccc
     2401 tgcgcctctc ctgcttcggt ttcatgagca tcaagcagct tccagtctgc aaatgaaaca
     2461 tggtctactt ttcttgattc cagcagagaa acaatgtccg gaagcaccac attttgaccg
     2521 gcttccattt tttctgccag gaaagtttcc agcatgcgat gcacggtttc aactgcatcc
     2581 tgtttattgg taccaatcac accgctggga ccacgcttaa tccagccggc aacatattca
     2641 cgattccgga gagaattgcc gttttccgga tctttgactt gtccgctttc atttggaatc
     2701 gttccggatt tttgatcaaa aggcaggtcc gcaagtggtt ttcctagata accgattgaa
     2761 cggaaaatca ggcctgcatc ttcttcaatt ttattttcag ttgcctgggg ccgcaaactg
     2821 ccgtcatcct gtttcaccag ttgatttcgt accatactta ttttttcaac cttgccgtct
     2881 ccggaaattt caagaggtga agcaagaaag aggaaatgga cccgtttctt ttttccagac
     2941 agtttcctca atgaaatttg tgtaagtatt tcaatgtttt tctttgtatc tttacttgct
     3001 tccgtttcca gaatcttctg gctttcttcg tccaactcaa gggaacctcc ctccaacata
     3061 acatcggcat catccaattc taaaaactct ctgacttcaa caggtgtgaa tgcagcctgc
     3121 agaggaccac gacgccctac cagccagata ttttcaattt gacttttacg cagtgcctca
     3181 agggcataat cggtaatatc agttgcagcc agttcttccg gagtttttgc cagaattcga
     3241 gccacatcca gcgcaacgtt tcccatgcca atcacaaatg cattcttgac agacaaatca
     3301 aacttatatt cacgaaaatc gggatgtcca ttataccagc caacaaactc agttgcagaa
     3361 tgactgccct ccaaatcctc acccggaatt ccgagcgcac gatcggatga acttccaact
     3421 gaataaatca ctgcatcata gtgtttaagc aaatccggct ggtaaaggtc actcccaaat
     3481 ttaacattgc caaagaatct gaaatttggg ttttccgcaa tgcgattgta gatttttgta
     3541 acagatttta ttttctggtg atcaggagcc actcctccgc gaaccagccc aaaaggagta
     3601 ggcagtcggt caaacatgtc aacttttatt tcccatgact gctgacggaa caactcccca
     3661 gttgcataaa aacctgcagg tccggaaccg attactgcaa tttcccagga tgtatccatt
     3721 tcttaagttt ttttatagcc taatttaaat ttgaaactgt cgtgaaaagt ccaaaaccgt
     3781 tttatttcag actttctgcg aaaccatcaa aaagatttat aagattctca aaaaaatatc
     3841 tttccagtga gtactggtac aaacaaagct ctcattcagc ttttattttt ctgcagcaac
     3901 agcccgaagt aaaatttcct caccgccatt tttcagcagc cactgtgcaa catcttttcc
     3961 caattgcagc gcattttgtc gtggtgcact tgcttcgtaa cgtaattgga tttttccttc
     4021 agaatcgcca ataaaggccc tcaaaaacaa ctcttctccg ttcagtatgc aatatcctgc
     4081 caaaggaagc tgacaatttc cttcaagttc tgccagcaaa gagcgctctg caagcaggca
     4141 gtcccaggta agcggatcat gaagatgctg aatccgactc agcgtagctt catctccagc
     4201 tcgtgtttca attccaatag cgccttgacc aattgcagga agcatccatt caatcggcaa
     4261 aatctcagtg attcgcttct ccaacttgat ccgctttaat cctgaaactg ctaaaatcac
     4321 tgcatctact tctccctgat ccagttttct gagccgggtt tcaacatttc cacgcaaact
     4381 catgacagac aaatccggtc gatgatgctg caattgtgaa gcacgacgca aggaacttgt
     4441 acctacaact gcaccgtctg gaaagttttc gatacttccg aattttggac aaatccatgc
     4501 gtctccagga tcttcccgct ctgcaatcac agaaatctca agtccctgag gtaaaaaact
     4561 agtcacatct ttcatgctgt gcacagccag atctgcacgt ccctcaagta gagcatattc
     4621 caattctttg acgaacagac cctttcctcc tatttctgaa agcggacggt cctgtatttt
     4681 gtcaccggta gttttgacaa ctaataactc aaccgaaatt tcaggatatt catcaagtat
     4741 cctttgcttc acccactctg cctgccagag cgcaagtgaa cttccacgag ttgcaattat
     4801 taaattggtt tgcttgttgt cgcttgtcga aaaagcgtta gacatgtgtc aggattttac
     4861 aattttaagc ctgtgatgtt tttccggtaa cttttccagc tttactggag ttggactcag
     4921 atcaaatatt tttgacacat attcaaggta gagatgtgca tcttcatcgt gtgcaatttt
     4981 tttcagattg cgggatggat catgcaaaag tctgtgaatc agcctgtgga ccagttgttc
     5041 aacttttttg cgctcagact caggtatgtt tttcaaattc ttaaaggatt tcaccatttc
     5101 ttcttcagca attgaatgga atgctttgcg caaactcctg attgttggaa ctgcagaaag
     5161 agacttaaac cagagttcca gctttagtaa ttcattttca ataatttctt cagccttaat
     5221 ggactgcttc tttcgttcaa cctgattttg agaaacaaga gtttgcaaat catcgatatc
     5281 gtaacaaaaa gttccattta actcatttat ttcaggatca atatcccgcg gtaccgcaat
     5341 atcgatgaag aacattgatc tgcccctgcg tcggcgtaag cagttcttca ccatttcctt
     5401 agtgataata aaatcacgtg ctccagttga actgagaata atatctgctt gatgaagatg
     5461 ccgctccaga tgctcaaatg gtacggccaa tccctgaaat ttttctgcaa gttttgctgc
     5521 acttaaaaaa gtacggttgg taacaaagag ctccgaaatt ccattgcgca tgaagtgacg
     5581 aacagccagt tctgccattt cacctgcgcc gataaccatt acagtctgtt ttgaaagatc
     5641 ctcaaaaata tgctttgcca gttccactgc tgcatagctg acggaaacag cgttactggc
     5701 aacactggtt tccgttcgca cacgctttgc tgtagaaaaa gctctagtaa aaagaggatg
     5761 gagcagtttt cccgtcaagt ctgcctcatt gaatgactga aatgcatctt tcagttgtcc
     5821 aagaatttgc ggttctccca gaaccattga gtctaaactc gaggcaaccc ggaagaggtg
     5881 acgtactccg tctaaatttt gaatggtata gactctttcc cttagttcgt aaaggttcca
     5941 atacgactcc agcatctcaa tgatttgatc aagctgctgt tctgcattta caagatataa
     6001 ctctgtgcga ttgcaagtac tgagaattac aactccaccc aggttaaaac gggtcctcaa
     6061 ctgtagtcca agctccactt gctcctttgg agtcaaagca agactatccc ggatttcaac
     6121 aggtgttttt ctaaaattcc aaccaattac agtaaaacgc atgtttagaa gtgtttttgc
     6181 aaagttgtta aatttttctt acttaaaaag tgactaaata aaatcaattt acactgaaag
     6241 gtaaattgct ttttttactg aataagcgga tcatacagtc ttgatgaaat aactcaagca
     6301 aggaataatt tttcatggtt gccttttttt ccaaatggct tactgccaat catttaacca
     6361 cattaagggt agcattggta ccttttattt acctgggtat ggtatttggg aaagacgatc
     6421 agacgatttt gttcagcacc tggctggtct ttttatttgc atgcctgacc gactactggg
     6481 atggtgtgtt ggccagacat caaggacaaa caaccaaact tggtaaatta ctggatcctg
     6541 ttgcagacaa aattttgatt ggtaccctac tggttttatt agtaggaatg ggcagagcac
     6601 cagcgttatt agcagtcatc ctgatttccc gcgaatttat tatcagcggt ttacgctcca
     6661 tcgctgcagc agaaggcgtg gtgatttccg ccagttccgg aggaaaactg aaaacaattt
     6721 ttcagatgtt tgctgtaggc ttcctcataa ttcactatcc cacattcagc attccatgcc
     6781 atgaagtagg aatcattctg ctctggattt ctacagcggt ttcactttgg agcggattta
     6841 attatgttgt ggcctatttt cgtgcttccc ctggaaaata actgccaatc cgattttcag
     6901 tttcagtgaa gaaatcatct ctcgtctgga cattaacttt ccaactgata ctatataatg
     6961 tgtctgaacg atgtaattaa ttttattgga gagtaagaaa attcgattat gaaatcgaag
     7021 atttgaaacg aagctgtcat ccgtcagcta ttcgtttttt acatcgtttt tcagacttat
     7081 agcaaacagc tcaaatctga cagctaagtc tttattcatt ttataacgat tgaaatattg
     7141 atcagcaccg gatcaagata ttttcggtaa atcgctattt agacagaaaa gtattttaag
     7201 atcactaaaa agtctatgac aactattctt tgtgttcgaa agaaaaatcg tgtagtgatg
     7261 gctggagacg gacaagtctc catgcaaaac accatcatga aaccttcagc ccgtaaatta
     7321 cgcaatacct ttgacggaaa ggtcattgcc ggattcgccg gttcaactgc agatgccttt
     7381 accctctttg aaaaatttga cgaaaaactg gaacaataca acggaaaact tctgcgctct
     7441 gcagttgaac tggctaaaga atggcgtacc gataaaatgc tgaggaacct tgaagccctg
     7501 ctggcagttt ccagtgcgga agcctctctg attatttccg gaaatggaga tgtgattgaa
     7561 cccgatgacg gtctgatcgg aattggttcc ggaggaatgt atgctcttgc ggctgcacgt
     7621 gcgctcattg atacgaacct cagtccccga aaaattgcag agaaatctat gaaaattgcc
     7681 tcagaaattt gtatttacac caacaaccag attacttacg aagaattgga attttaagct
     7741 catggattct gctcctgaac tcaacatgac accgcaagag accgttgacg cgctcgaccg
     7801 ttatatcatc ggtcagactg atgcaaaaaa agcagtagcg gtagctctgc gtaatcggtg
     7861 gagacgcctg cagctggatg aagatttgaa agacgaaatt ttgcctaaaa atatcatcat
     7921 gatcggtgca accggcatag gtaaaactga aattgcacga cgtatttcaa agctggtgaa
     7981 tgctcctttt gtgaaagttg aagcatccaa atttactgaa gtgggttatg tgggacgtga
     8041 tgttgaatca atgattcgtg atttgacaga ttattcagta aacatggttc gtcaagagca
     8101 aatggaacgt aaacaggaag cagccaaaga aatggttgaa gagcgtttgc tggatctgct
     8161 gctgcctccc ttggaaagtg agaaagatgc ttctgaagga aaagcttcaa caaatcctac
     8221 tcgtgaaaaa tttcgtaaaa aacttcgctc cggagtttta gatgagcgaa tggttgaact
     8281 ggatgtttca gaacggcctt caggggcagt tatggagttt atgcctgttt ccatgaatga
     8341 caatatggat atgaatatcc gtgatatgct ctcaaatatg atgcccaaac aatcaaagcg
     8401 tcggcgggtc aacattgagg atgcaagaaa aatattgcag caggaagagg tcagcaaact
     8461 catcgacatg gatgaagtca tccgggaagc gttaacaaga gttgaacaga gtggaattgt
     8521 ttttttggat gagattgaca aagttgcttc acgccgaagt cactctcatg gaccggatgt
     8581 ttccagagaa ggagtacagc gggacttact gccgattgtt gaaggttcga ccgtaaacac
     8641 caaacacgga cctgttcaaa ccgatcacat tctgttcatt gccgcaggtg catttcatct
     8701 aacaaaaccg tctgacatga ttccggaact acagggtcgt tttccaattc gtgttgaact
     8761 gcagtcactc agtaaagacg attttgtcaa gattctaaca gaaccaaaaa acgcgctgat
     8821 tgttcagtat actgccttga tgaaaacaga aggtgttgaa ttggaattta caaaagccgc
     8881 cattgatact attgcggaaa ttgcctgcca aatcaacgaa gattcggaaa atatcggtgc
     8941 acgccggctg catactgtaa tggaacaatt actcgaagaa gtcagtttta ctgccccgga
     9001 tttaaatgga cagaaaatat ctgttactcc ggaatatatt cagagccgac tctcaaactt
     9061 gttgaaaaat caggatttga gtcgttatat tttataaaat aaattcctaa aaagttgtaa
     9121 cttatagagg aaaaactttg gattatccta ctttgctgct gaaattagaa aatcagttgc
     9181 agtctcttca taaaaaaggt tcccattaca tgaacattga agctatgccc aaggttggac
     9241 tcaggcgtct gcttgacggt ttgggcgaga tactggattc tccaactgaa gatgtagaca
     9301 aacggtccaa atacaacttt tcggtttcag cagcaactca gcctgcagtc aaaattaagc
     9361 acgaaaatgc tgattccaaa atatttcctc cgtcaacagg cataaccgat cttcaagaaa
     9421 aattgtcagc ggcaaagcaa caaactgtct tagaggcttt caaacaatta atacctgaac
     9481 cggaagcaga aataaaagta agttctcttt cagagttggc agataagtat aagtcctgtc
     9541 aacgttgttc attgggtaaa accagaacaa atctggtatt tggtaatggt tcgattaatt
     9601 ccagggtgat ttttctagga gaaggccccg gtgcagacga agacgctcaa ggtcagccct
     9661 ttgtaggacg tgccggcagg ctgctgacca aaatgatcaa tacaataggc attgaacgcg
     9721 atgatgttta tattacaaat attgtcaaat gccgccctcc ggaaaacagg aatcctactc
     9781 ctgctgaaat ttcatgctgt ctcccaatta tcaaacagca acttgaatta ttgaatccaa
     9841 aattaatcgt cactcttggg aatattccaa ctaaatcact gatcccggat ttacccggaa
     9901 tcaccaaggt acgtggtaga attatgcaat atgaaaattg gaccgtgctg cccacctttc
     9961 atccttctta cctcttgcga aaccgttctg caatgcctct ggcatgggag gattttaaga
    10021 aaataccaga actggctttt cattcgtgat gcggttcaaa cagcagatat tcaattttca
    10081 acaacagtcc acagataaac atttataatg attgatttgc agcctgtcaa gggtacgagg
    10141 gacttctttc cggacgaaat gcgtttacgc aactggctat ttgaagtgtg gcgcaatgtc
    10201 tctgttcagg caggtttcga agaatatgat acttgtgtgc tggaacatga agagctttat
    10261 attcgcaagg ccggagacga aatcagcaag cagctttaca gctttgaaga caagggtgga
    10321 cggcgtttaa gtttgcgtcc cgaaatgacc ccttcattgg cccgtctggt gcttcagcac
    10381 aaaaaatcat tgtcctttcc aataaagtgg ttttcaatgc cgcaatgttt ccgctatgag
    10441 cgcatgacaa aagggcggag gcgcgagcat tttcagtgga atgcagacat cattggacaa
    10501 gctgagattg tggctgaagc agaaattctg atgctgttga tttctgcatg cgaattaatg
    10561 ggactttcag caaaagaaat ccgtgtgttt atcaatgacc gccggattct gaactcgatt
    10621 ctaagtcaaa ttagtgtacc tcaggaaatg cacagtgaag tacttgtcat aatggataag
    10681 cgcgataaaa tttccataga agcactgtca aaattgctgc aggatattgg aatgtccaca
    10741 actcaggtgg accagttaaa tgaatattta ttaaaatcag acttgattga attgaaaaaa
    10801 gacctgaatg acactcaggg tattgacgaa ttgcaaaact tgatggatat gatggaaaca
    10861 gcaggattca gtgattatct gcaatttgat atttccatcg tcaggggttt atcttattac
    10921 acaggtgcag tctttgaggt taacagtcca gcaaaacaac accgggctat ttgcggcggc
    10981 ggacgctacg attccctgct ctcaacatat ggcggtgaaa ttgtacctgc agtcggattt
    11041 gggtttggag acgtggtggt tttagacgta cttaaagatt tggagcgctt tcctgaatta
    11101 ccacggaaac tggattacac tattattcca tttgccagag aacaggtggg tactgctctc
    11161 aatattgcag caatactgcg gcagcagggt tcaactgtcg actgtaactt tagcatgaaa
    11221 aaaatgaaaa aaactttgca gcaggctggt gagtccggag cagcaaaagc gatccttctc
    11281 tttccggatg agttatctga aaacaaggtg gtaatccggg atttgcggct tcatgaacag
    11341 aagccaatta aaataaccga tctactttct tctggggagt gaaaaatttg gctatttcag
    11401 aatcagtttt ttcaacttct ttcaggacca actggattat aatgatcatc tttttgggct
    11461 tggatcttag ttcaaaaata tggatcaggt taaacgtacc tctgtatgaa acaaccgagc
    11521 tactggcaaa tatattagac ttgacccacg tgcagaatcg cggtgtaagc tttagttttt
    11581 tggcagactt ttctgattct tatcgagttc ctctcctggt tggagtgtca ttgctggctg
    11641 ttgcagcaat gctgtattat caaacacgct actggagcga acttgatttt ttcacacggt
    11701 caggattagt cttggtatta ccgggtgcga cagggaactt gattgatcgt gcagtttatg
    11761 gctacgtcac tgattttttg cattttcact ggtatgaaac cagttttttt gtcaataacc
    11821 ttgcggattg cttcatcagt ctcggtgtaa tttttttcat tgttccactt ttgttttcga
    11881 aaaatggaag tacttcatca aactcagctt aattcatttt tgtttagtaa aataaaattc
    11941 gtgctgattt tttctgcact ttttttatcc tctaatttta ttgtttccgg aatctttgca
    12001 aaggaagaaa tacgatatgt caaaggactg ggagcagtcc tgcgggaaca accgtcaatt
    12061 ctgagtaaaa aactcacaaa actttctcgc ggcacaaaag ttctggcatt agaggcaaaa
    12121 ggtatttggc ataaagttat tgcaggagaa attcaaggct ggcttttgcg gggacagata
    12181 acaaatattc ctgttaaaat caaaaaatta gttttgggac aaaaaattcg actgacttcc
    12241 tcttcaggac ggagggtacg cttacgtact ttttctgcag tagttggagt gaggggtctg
    12301 gttgacagta aaggggaaaa aatcagcccc tatcaaacag attatgtggc actggaatgg
    12361 ctggaaaaac aacctactga tgaagaaaat tctgttcgat ttttaacatt atcagagtaa
    12421 agactatatg aattttttcc gtaaccatct ttggttttat atttttatta tttcttttgt
    12481 ctttgcaagt tttggaaatg cacaggaatt ccgccttaga gcacatcatg tatattggaa
    12541 aaatcaggaa gcaaatactg agcgggaaat tgatttcgga cgcagtgttt ctgctaaaat
    12601 tcttggtaag tatccaattc tgcggaatga acaaaaggta cgttacatat cccaaattgg
    12661 tactggaatt tccgcacaac tcgggcggcc tgaattaaga tactattttg gaatactgga
    12721 tacggatgag gtaaacgctt ttgctgctcc gggaggttat atttttgtaa caaaaggtgc
    12781 cttgaaactt atgcgtaatg aagcacagct cgcaggtgtt ttggctcacg agctggcaca
    12841 tattgatcaa aaacacatag ttcgaaaact caaacttcaa agcacagacc gttctgttac
    12901 ttctggaatt gcacaggttt taggaggagc atcactttct gctaaaatcg ctcttgaacg
    12961 gctcaatgac cttgcattta aaatgctgat ggaggaaggt ctttccaagg aagatgaagc
    13021 ggatgccgat caaaaagcat tggaaatgct gatatccacc ggctacgatc tgcaaagcta
    13081 tctggattat cttctgtccc ttaaaccata tttagaacag ggacaggcaa aagttctttc
    13141 aaaaactcac ccgacaattg aatttcgata caacaattta cgtgagttta tcatcaagaa
    13201 taacctcgaa aaatttcagg gtaagaataa tgaaatacgc tttaagacaa atttcgctaa
    13261 gctttagtat attttttatc agttgttttg tcgcaactca gttgtatgca gatgttgatc
    13321 attacaaaaa tatgctggtt ggtgaacgtg cggcaacaat gggtggaacc tacgtagcta
    13381 tttctgatga cagcaccgga tgctactata atcctgcagg tatcgcctat gcggttggcg
    13441 acagtctttc gggcagtggt aacgtgctgc acaaaatgaa aactgtctac tctgagacaa
    13501 ttggtaccga agattgggtc cgtgaaagcg aaacgcttgt gcctaactac tttggcgtac
    13561 tcaaaaaata caaatcttac tcgttttgct tttcatatgt ggtcccggaa gcttttattg
    13621 aacaccagga tctggtattt aatgatcctt taagtactgt caaaatatat tatcaaagcc
    13681 tgcattcaga agacattaca tatttgatgg gaccatcggg tgcgctgcaa tttggagaaa
    13741 atttttcttt aggattaacg ctttattatc attaccgcag ctttatgcgt caatatcatt
    13801 atttcctgaa aagtacagac ggcggctatc aattatatta tgaaagtaat aaacaacgag
    13861 aagacggttt gatgccaaaa attggtgtgc agtggagtcc atggagcctg ctgacagtag
    13921 gaatggccct ggatcagaca tttttattta atgccacttt tcagggaaat gcctctatac
    13981 acaatacaga caacagtaca ggaacagcaa gctataccag cttgatgacc cgcacaattt
    14041 cagaagaaaa acgtaaattt cctctgcacc tggcctttgg tgcagcttat tttcctacac
    14101 catctctact ctacaccgtt gatcttgatt attacaaggc acagcaaaag ggaagagcgg
    14161 atgtaatcaa ttattccggc ggtaccgagt attatttcga cccgacaaat gcagtgcgtt
    14221 tcggtttgtt caccaactac actaaccttc cgcaaactga ttccacaaca atagctccgt
    14281 atgaatacat agacatttat ggagcaagtt ttggatacac ttcctattcg tcctcctcgt
    14341 cactcacttt aggtgcgatc tacacttccg gttcaggaaa agcctggcta tactccggaa
    14401 gtacagaaac aagaaatatg aagcgtgatt cactcacact tttgttttca gcaagttcaa
    14461 gtctataagg ctccgctgca actgcatcat tgctaaactg caggaattgt cctgatctgc
    14521 agggatgtac aatagccttc accagtgagg atattggcaa gaacaactac ctgctcttca
    14581 gcagcaaaac cttccttttc ctggagaatt cccaaagctt tctgtattgt gtcttccgga
    14641 tttttccgga attccatcag gaaaggatag acactcctgt agagcgtcat tgaagaaaat
    14701 actttagaat tattagtgaa tgagtaaagc ccaatctcgc gtggacgcag atttgagaga
    14761 tattctgcac ctcgtccgtt gcgggtgata acaacaattg cgcgtatttt taattttgcg
    14821 gcaatttgga ttgcagcttc agcaaggtgc tgacctttag acttttttat caatttttca
    14881 gtataattca caccgggata attttctgta cgccttgcaa tccgtaccag agtttctacc
    14941 gaacggacag gatatttacc gacagaagtt tctccggaaa gcataattgc atctgtctgt
    15001 tcaaaaacag cattagcgac atcagtaact tccgctcggg tcggtactgg attttcaatc
    15061 attgattcca acatttgagt tgccactatt aaacgctttc catatttagc acacaaataa
    15121 gcaatccgtc tttgaatttg cgggacctct tccagctcaa cttcaattcc taaatcacca
    15181 cgggcaacca tgactccatc agcttcctgg acaatttctt cgaggcgctc aacaccctcc
    15241 tggttttcaa tttttgcaat aagcttaatt tgctgagcat gcactccaag tgaagccctc
    15301 acctcccgga ttgtttccga actgcgcacg aaagacagag caatgacgtc cactttttgt
    15361 tcaattccaa aaaggatatc ctgtttatct gtttctgtga ttccaggcag tttaacgatt
    15421 gctcccggca aattcacatg tctatgggcc ttaacgacac cgccgtcaat cacttcgcag
    15481 tcaagccctt ttttgttttt accacaaact tttaaactta tcaaacctcc gtcaagggaa
    15541 atacaacttc cctctttcag atcatctttt aaatcaacat agttgacaaa aagtgtaccc
    15601 tctgactctt catttggagg aatagtgaca cacacacgtt ttccggaaat aagcgttatt
    15661 accttatcac cggtcctgat ttcaggaccc tgggtatcaa gcataattgc aattgggtat
    15721 tgcctgtcct tattcaattt tcgaatttgc tcaattattt ttttatgaga atgatgcgtt
    15781 ccatgggaga aattcaaacg cgcaatattc attcctgcct cagccaactc cgccagcttt
    15841 ttaggagagt cactggcagg accaatggtg caaataattt ttgtacttag cattttatgt
    15901 tttctttgat tgtaatccgg caggattcag atattattca gtctatgtat tgaccgtctc
    15961 tgaaacgttt atactgaaga tactcttgaa aaatataggg tttgtagatc gattttccta
    16021 ggagcattcg aatacattcc tgaatgcctt caataataga cgggtgaggg tggatcaatt
    16081 ctgataattc ttcaataccc ttatttgtgg caataagtaa agcaacagcc tgaattgcac
    16141 tggacgcatg ttcaccaact gcacgtaaac cgaggacttt catttgatcg tcatctgtta
    16201 caaggatttt aaaaaaaccc tgagtgttgc gcatggcaat agctcttgga atacagcgaa
    16261 aatcatagct ggcaacacga taatccagtt cagctttttg agcctgctgt tcattcatac
    16321 ccactcctgc aacctcagga ttgaggaaca tgattgttga aatattttcg tagatcactg
    16381 atctttttgg tttgccgtaa attttttcaa cagcatgacg tccttccaat tcacccacat
    16441 taaccagtgc aatgtctgca gtaatatctc ctgctgcata aatgttggaa atactggttt
    16501 gtgtgtcatt atcaataatg tgtcctgatt cactcagttc cattcctatt tcgtgcaaac
    16561 cggtattttc aatattcggt acacgtccta ccgaaaccaa agctttttct gcatgaaaaa
    16621 cctctttctc tccattcgga tgctcaagaa catattcaac ccctccattc gtaatttcca
    16681 ttttatcaag ttttgattca tgatgaatga gcactccatt ggcctctaaa tttgcttcaa
    16741 tcactttggc caggtccgga tcttcaaaag gtaaaatccg atcttctttg gaaataatat
    16801 gaacttttgt ttttccgaaa ttggaaaaga tagtggcaaa ctcacagccg attactcctg
    16861 ctccgagaat tacgagactc tcgggaaact cttccagcga actgatgcca tcgctggtca
    16921 tgatgatatc ctcgtcaatt gtaattgagg ggattttacg gggacgactt ccggtagcaa
    16981 ttatgatatt ttccgcattt accttttctg taccggagtc ggtttccagc tcaatttcac
    17041 ttgagctgat taaatgagca cttcctttga tgtagcgaaa gccttccgga cgttgttgaa
    17101 ttaccagtcg gaggtgatct tctagttgat ttgaacgctc actaaccgca tcattcactt
    17161 cattcagcac attgtgaaat tgcacaaccg gttcttccaa cccgtagtga ctcatctttt
    17221 tactgaatga agcaaattct ttggatattt cccaaaatgt ttttgaggaa agtgcaccat
    17281 catatattcc tgcgccgcca attcgatccc gttcaacaag taaaacactt ttatcaaaat
    17341 caactgcacg catggctgcc gcatagccgg acggtccacc gccaattaca cacagatcat
    17401 atttttccat tgaatagatt tttcttataa ttactttgtg aaattagggc tagattgcag
    17461 ccctgcaaca ccgctatttc ttttgaagtt tcctgtacca aggtacaaag atcttgaact
    17521 gctaaaaat
//
LOCUS       DTVD01000233           17477 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_594_length_17477_cov_390.036448, whole genome
            shotgun sequence.
ACCESSION   DTVD01000233 DTVD01000000
VERSION     DTVD01000233.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17477)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_17477_cov_390.036448"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(501..2168)
                     /locus_tag="EYO68_08400"
     CDS             complement(501..2168)
                     /locus_tag="EYO68_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HIC07864.1"
                     /translation="MQKSLNLKSSPLSENWWESAVFYQIYPRSFKDSNNDGIGDLAGV
                     IEKLDHLNDGKGGGLGIDAIWFSPFFPSPQADFGYDVSDYCNIDSDYGTLEDFDQLVE
                     ESHRRGIKIVLDLVLNHSSDQHKWFQESRKNSTNSKVDWYVWADPKPDGSPPNNWLAV
                     FGGAAWTFEPQRGQYYLHNFLPEQPDLNWYNPEVREALADVVRFWMKRGADGFRLDTA
                     NYYAYDRQLRDNPKRPENSELMEDGQEANPLSQYITKYSKDRPENLEFIHFLRKIFDE
                     NGAVSIGEIGSAEGLESTLKLGTDYVKNGKGLHLAYTFSMLNKNMNAEYLEHVLRVTE
                     ESIEDGWPCWSLSNHDCMRMISRFDCFGERDGFQQMMLLLLLSLRGTPIIYYGEEVDM
                     QEYEITKDELRDPQGIRFWPDIKGRDGCRLPFPWDSKLTNQGFNSGTKPWLPAVNKLS
                     LDQAKADSGSTFHVLQEMLQIRKKFPALQNGSYRKILLDGDCFVFERKTEDETMLIAA
                     NFSTEEQSINLPYKVTKDHTPKAFKRFCTLKNELLTSPACGYFLGEK"
     gene            complement(2214..3362)
                     /locus_tag="EYO68_08405"
     CDS             complement(2214..3362)
                     /locus_tag="EYO68_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC07865.1"
                     /translation="MTFRKPQHLTLGRLFNHLALLVLVLLWILPTIGLLVSSFRDKDQ
                     LAYSGWWTALTTSEQNDWGRTGKAEDQIEKSGNYVISGTILEPGEKTILTFNTNFRNL
                     SAYKSGETMIFKDDSHFTLQADGKYEWVSQKPFTHKRGKRIFYVANVPPSFSLDNYRE
                     VLASENVGQSFVNTFTVTIPATIIPIMIAAFAAYAFAWMEFPGRQFLFVCVVGLLVVP
                     LQMSLIPLLTMYNKIGTFFGTEAKSYLGIWLAHTGFGLPLAIYLLRNYMGSLPRDIIE
                     SARIDGASHFQIFTRLVLPLSIPALASFCIFQFLWVWNDLLVALVFLGKQDNQIVLTS
                     KLRELLGSRGDNWEILTSSAFISIVVPLLVFFALQRYFVRGLVTGSVK"
     gene            complement(3359..4363)
                     /locus_tag="EYO68_08410"
     CDS             complement(3359..4363)
                     /locus_tag="EYO68_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC07866.1"
                     /translation="MLEQLLNALLAVFLGVGACTLYYVGSNFLLDQVFADSGENAVRN
                     SRQRAAIRPWLFLAPALLILSIYLVYPVLETLRLSFYNFGGFEFVGLKNYIWATKNPE
                     FLQAIGNNFMWLIVVPTLSVAFGLAVAVLADKLWWGTIAKSLIFMPMAISFVGASVIW
                     KFVYDYRGPDTDQIGSLNAFVMMFGFEPQAWITLPFWNNFFLMVILVWIQTGFAMVIF
                     SAALRGIPEATLEAARIDGAGEIQIFFKIMIPQIMGTIMVVWTTITIIVLKVFDIVLA
                     MTNGQWNTDVLANLMFDWMFRGGGDSGRGSIVALMIMMAVIPIMFWNIRNFRKEEEAR
                     "
     gene            complement(4441..5736)
                     /locus_tag="EYO68_08415"
     CDS             complement(4441..5736)
                     /locus_tag="EYO68_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07867.1"
                     /translation="MKGALLAVVATALISVPAMAGEYKGPSLSGQTVTISGPWLTADE
                     ESFEAVIAYFEKATGAKVEYAGSDSFEQQIVIDIKAGSPPNLAAFPQPGLAANMAAIG
                     GLVPLGSSTANWVKSNYAAGQSWVDLGTYPSKSGKKEFYGFFFNVNVKSLVWYSPENF
                     EAGGYSVPNTMEELIALSDQIVKDGGTPWCIGLGSGDATGWPATDWVEDLMLRTQPPS
                     VYDGWVTNDVKFNDPRVVAAIETFGKFAKNSKYVDGGMAAVGSTDFRDSPKGLFTVPP
                     RCYMHRQASFIPAFFPKRVKVGEDADFFYFPSYSTKKLGNPVLGGGTLLAMAKDSKAT
                     REFIKYLQHPKSHEIWMARAGFLTPHKGVDLSKYSSGTLRKQGEILQNATTFRFDGSD
                     LMPGAIGAGAFWSQMVYYVSGASAKKVADNVQSTWDSIK"
     gene            5916..6026
                     /locus_tag="EYO68_08420"
                     /pseudo
     CDS             5916..6026
                     /locus_tag="EYO68_08420"
                     /inference="COORDINATES: protein motif:HMM:PF00356.19"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            6123..6914
                     /locus_tag="EYO68_08425"
     CDS             6123..6914
                     /locus_tag="EYO68_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07868.1"
                     /translation="MLAGISVSLSKVGYDLNISVAPNSEHEIDSLRRMVQGKRVDGII
                     LTLTKAVDERISYLLDQNFPFVLYGRTEEPRPHAFLDIDGELAFREACTFLIELGHKR
                     IALINGEPEYMFPAFCSEGYRNGLKSGNLPFDPELVREWRHGDAQLSSFQQTVELMQL
                     SEPPTAIVFQTEAAAGILQALLQLQLQVGKDIALIGYDDLEIAKTNIPPLTTMRPPAR
                     NAGEQFVGMLMQIIDGRAAEDLQEVWKAKMVMRQSTGRISCNDST"
     gene            7015..8112
                     /gene="ugpC"
                     /locus_tag="EYO68_08430"
     CDS             7015..8112
                     /gene="ugpC"
                     /locus_tag="EYO68_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HIC07869.1"
                     /translation="MAEVKMQSIYKQFGNTEVIHGVDLEVDDNEFVVLVGPSGCGKST
                     LLRLIAGLEDVTSGEIEIDGVRVDYLPPAKRGIAMVFQSYALYPHMNVYQNMSFGLRL
                     AKTDKQIVDQKVRDAAEILQITELLERKPKELSGGQRQRVAIGRAIVRDPKVFLFDEP
                     LSNLDAKLRVQMRIELTKLHRELNATMVYVTHDQVEAMTMADKIVVLREGYVEQVGAP
                     LDLYHNPANLFVAGFIGSPAMNFMDGKISGISDQAVEFELNSKQKIQIPCQPGEQAAA
                     GSSIKAGVRPEHLELTGDSDAILQGEVFAVERLGGETYLYVQTERNNELTVHATGDKI
                     VSVGDSVSIGFAPESCHLFDGQGQVFEKLAP"
     assembly_gap    8232..8331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8332..9113)
                     /locus_tag="EYO68_08435"
     CDS             complement(<8332..9113)
                     /locus_tag="EYO68_08435"
                     /inference="COORDINATES: protein motif:HMM:PF13452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC07870.1"
                     /translation="MNSEYNEWLGKTEVFHEQISEVNAKRLHHTLTQNGNPPSSGEAV
                     FPLFLLTLGEPSVPPNQLGEDGHPKRGSFMPPIPLKRRMFAGAEYEFHRALRVGDEVK
                     TTWNITDITRKNGSSGELVFINILREFHVRETLCATENRNIVFTDSDPKIRELNDPEE
                     SGEWMEEMSTNPLQLFRYSALTFNCHRIHYDRAYATEVEGYPGLVVHGPLLATWLSLF
                     VARKSGRKLKKFRFRAKRPVFDLHHFNLTGDLSGNDAAKLRVL"
     gene            complement(9132..10235)
                     /locus_tag="EYO68_08440"
     CDS             complement(9132..10235)
                     /locus_tag="EYO68_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIC07871.1"
                     /translation="MLVVSLEQAVAAPFVSSRLADAGARVLKIERPEGDFARNYDHVV
                     NGQSAYFVWLNRGKESISMDLKRPEEIQLLCSILQKADVLIENFKPGSLKRLGLDFNK
                     LRETNKKLITCSISGYGSQGEWAKMKAYDLLIQAETGLASITGSADAPGRVGISVADI
                     ATGMYAWSAILEALIARGINEHGQHIEVSLFDALADWMAVPLLWQDYGGKAPERVGLS
                     HPSICPYGAFKTADGKTILLSIQNEREWQRFCVEILVDADFAEQYPDNSSRLANRSVV
                     DGCVETALGKLSHTEASAKLKKSEIAYSTLNDVQGLSSHPILRRAVISNPEGKINVPA
                     PPAIFNDAPQDSLKEVPSLNQHGAAIRAEFSEN"
     gene            complement(10253..>10867)
                     /locus_tag="EYO68_08445"
     CDS             complement(10253..>10867)
                     /locus_tag="EYO68_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIC07872.1"
                     /translation="LSVFIVDMRNANGNGLTIRPIRTMINHATTEVFFDDLRVPQENL
                     IGEEGKGFRYIIDGMNAERILIASECIGDSRWFIEKATAYANERIVFDRPIGKNQGIQ
                     FPLAQAYAATEAAALMTEKATLLYEDGKRPGAEANMAKLLASDASWKSAEACLQTHGG
                     YGFAEEYDVERKFRETRLYQIAPISTNLILSYIAEHVLGLPRSY"
     assembly_gap    10868..10967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11034..12518)
                     /locus_tag="EYO68_08450"
     CDS             complement(11034..12518)
                     /locus_tag="EYO68_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010443812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIC07873.1"
                     /translation="MADLKKYQMYIDGQWVNSESEKTFESFNPATEEPWAIIPEAGAN
                     DVDRAVKAAHRAFTEGPWANMTATERGKLLRRLAEVLAEHSESLGRAETTDTGKLLKE
                     TQWQARYISDFFQYYAGLADKVHGDTLPIDKPNMWTMTVREPLGVVAAVVPWNSQLFL
                     VAVKIGPALAAGNTVVLKASEHASAPMLEFAKVFEEAGFPPGVMNIVTGYGDPCAKAL
                     TSHPLVARVSFTGGPETARDVVRNSAENLAQVSLELGGKSPMIVFEDADLESATNGVL
                     LSIFSASGQSCVAGSRLLLHESIHDKILDQVARRAKEIRIGDPLAETSQMGPLATLAQ
                     LNNIQSTVADATANGATLIHGGGKPAHLEKGWYCEPTIVDCPNQNLGIVQREMFGPVV
                     SVLRFRDEAEAIKLANDTRYGLASGVFTTNIGRALRVTKALRAGIVWVNTYRMVSPLA
                     PFGGFKDSGYGRESGLDAIYDYTRPKTVWLNTSPDPIADPFIMQ"
     gene            complement(12543..12935)
                     /locus_tag="EYO68_08455"
     CDS             complement(12543..12935)
                     /locus_tag="EYO68_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIC07874.1"
                     /translation="MKTGKEIIGGPLIINGRQLTLSKAVRAGDFIFLTGQVPMKDGAP
                     MTEGTIEEQTRVCIELIRETLKDAGCTLNDVVKSMVWLKNRDDFPGFNSVYGEYFSEG
                     PPARSALVCDFLIDIKVEIECTAYKPEN"
     gene            complement(12965..13765)
                     /locus_tag="EYO68_08460"
     CDS             complement(12965..13765)
                     /locus_tag="EYO68_08460"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIC07875.1"
                     /translation="MNDIKHGSFQGTSWSKEGTGSPVILVHGLGMNRQMWQWQMDSLT
                     PHFSVICYDLLGHGESVNPEIPCQLSQFSGQLLALMDGLNLESCALVGFSLGGLIVQD
                     FALNHPEKVNALVMLNTAHGRTKSERKAVLARVQQAGEHGPASTVEDALERWFSADFS
                     AKNPEILDKIRCWIMANDKEIYPEIYRLLAECDEPLTDSISAIRCPTLVMTGEDDHGN
                     SPEMTQRMAALIPDARAVILPGLRHMGLAENSQAVNSQLVSFFQEVLK"
     gene            complement(13780..14943)
                     /locus_tag="EYO68_08465"
     CDS             complement(13780..14943)
                     /locus_tag="EYO68_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIC07876.1"
                     /translation="MDFGYFANVSNPGLVKPYGQILGEVRDLAVTIEDAGWDSIWFTE
                     HHFGHEGFEVCPNAIMLCTDIAARTSRIRLGQAANIVTFWNPLRFVEDIAVLDHMSNG
                     RVEVGIGRGVYGREASNLNKDADLKDQAKNFRLFAETVELMKKAWTQDFISHQGEFFT
                     YSEPGLIWSHPDSPKSETFMNMETNEIEKISILPKPLQKPHPQLWQVVDSTSSIEWAA
                     KNDINVIMWIPTVKTLKKRFEIYRDARSEKLGREVPLGEGISLVRDMFCAENMEDAQR
                     LGGDGILKYLKWICHWRGLGNHLDPGEELPDTQGKLDALTYEWLHPRNMLFGTPEYIV
                     EKIKELQSELNLQSLMLWSHFPDVPHETAMRSIKLFNEEVLPHFKSGTVTLAN"
     gene            complement(15049..16020)
                     /locus_tag="EYO68_08470"
     CDS             complement(15049..16020)
                     /locus_tag="EYO68_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HIC07877.1"
                     /translation="MTQEFSGKDPKSVQKVTSIGAGPIGAGWAAYFLSQGYKVSVYLH
                     EESEKNVLHNLINTAWISLEALGLAEGASRDNLYYSTDLADAVNGSEFIQESVPENLA
                     LKQKIFAQLGELVPENVVIASSTSGLPMSEIQAHCSTPQRTVVGHPFNPPYLLPLVEI
                     VRGSQTSAEAVKWLVEFYQVAGKAPLVLSKEIPGFIATRLQEAIWREALHMVDNDEAT
                     VEQIDFAITNGPGPRWALMGPCMAFHLGGGEGGMAYTLDQFGPALKLPWTRLKAPELT
                     KELRNKMVDGCLREADGREISEMNRDRDRGLVAIRNALKASGSEVDS"
     gene            complement(16051..16848)
                     /gene="caiD"
                     /locus_tag="EYO68_08475"
     CDS             complement(16051..16848)
                     /gene="caiD"
                     /locus_tag="EYO68_08475"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HIC07878.1"
                     /translation="MEEVTVVKKGRVLEILLNRPKVNAIDLATSRKLGDAFVLLRDDP
                     ELWVGILTAAGDRIFSAGWDLKSLNQGEMSLDDWWENDYGAGGFAGLTEMWDLNKPVI
                     VALNGLAIGGGFEMAMACDLIIAAEHVEFGLPELPLGIVPDAGAIQRLPRRIPYNVAM
                     EMYLLGRRMTASEAQGYGFVNKVVSKEILMETAREWANQIAESAPLALQTVKEVLRAI
                     EGDTIEQSFQTMRKADLPVYRKMLGSDDAKEGVKAFVEKRKPDFKGL"
     gene            complement(16902..>17477)
                     /locus_tag="EYO68_08480"
     CDS             complement(16902..>17477)
                     /locus_tag="EYO68_08480"
                     /inference="COORDINATES: protein motif:HMM:PF13549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIC07879.1"
                     /translation="KIGFPVVVKMVGKNLAHKTDAGAIALNLLSCEEVKIAVDRMKKD
                     VEHFDPAAVSEKFLVEKMLERPIAELLVNVRTDPQFGMAMTLAAGGILTELLADAVTI
                     LLPTNRNDLENALKSLKISKLFDGFRGGEMIDTEQLVTALLNLVVGVMNPAIGIKEIE
                     INPLFVYKEKVCAVDVLMRIATGTSGAVNNK"
BASE COUNT         4829 a         4097 c         3565 g         4786 t
ORIGIN      
        1 gctcccttgg atctacactc aggacactaa tttttagtcc ggataaatat agagatttac
       61 ctttcaaaga tcaagcgctt ttatggcagg ctgagattcc tgtgtgatct tcacaacttg
      121 gagctattac aaataaattg ggagtgatcc gtacttctga atcaggtaaa atatagattt
      181 atgtatggag catgaccaaa ctacatagtt tgcattgctc attgttgtcg gtctggagga
      241 agggaagtgc ctttgcggag ttaatgtgtc gtggagtgac catctgagtt tggcagcaca
      301 gcggaacata tttggaaaca caacaaagtt ttacagtaca cgaagaggat ttcatgtcaa
      361 aatctacaac gataacaaaa ttacaggccc tgttccgctc tggagaggta agcaactatt
      421 gcttttttga atttaaaagt attactttct acaccagctt tgctataaaa gttttttggc
      481 aaatatgcta aaattactaa ttatttttca ccaagaaaat aaccgcatgc tggtgatgtc
      541 aaaagctcat ttttaagggt acagaaacgt ttaaatgctt tgggagtgtg gtcctttgtg
      601 actttgtatg gcaaattaat actttgctct tctgtagaaa agttggcggc aatcagcatg
      661 gtttcatctt ctgtttttct ttcaaaaaca aaacaatctc catccagtaa tatttttcga
      721 taacttccat tttgaagtgc tgggaatttt ttgcgtattt gcaacatttc ttgaagaaca
      781 tgaaaagtac ttcctgagtc tgcttttgct tgatccaatg acaatttgtt tacagccgga
      841 agccagggtt tggttccgga attaaaacct tgattagtca attttgaatc ccacggaaac
      901 ggaagccgac atccgtcgcg tcccttgata tccggccaaa aacgaatacc ttgaggatct
      961 cgcaactcat cttttgtgat ttcatattcc tgcatatcaa cctcctcacc gtaataaata
     1021 atcggtgttc ctcgcaaaga caaaagcaat aaaagcatca tttgttgaaa accatcacgc
     1081 tctccaaaac aatcaaatcg gctgatcatt cgcatacaat cgtgattgct caaagaccaa
     1141 caaggccagc cgtcctcgat tgattcttct gtgactcgca gcacatgctc caggtactca
     1201 gcattcatgt ttttgttcaa catcgaaaaa gtataagcca aatgtaatcc cttgccgttt
     1261 ttaacataat ccgttcccaa cttcaaagtt gactccaagc cctctgcact cccaatttct
     1321 cctattgaaa cagcgccatt ttcgtcaaat atttttcgca aaaagtggat gaattctaaa
     1381 ttttcgggac gatcttttga gtattttgtg atgtattgac tgagcggatt tgcttcttgt
     1441 ccgtcctcca tgagttcaga gttttctggt cgttttggat tgtcacgcaa ctgccggtca
     1501 taagcataat aatttgctgt gtccaaacga aatccatctg ctccgcgctt catccaaaag
     1561 cgaaccacat ctgccaaagc ttctcgaact tccggattat accaattcaa atccggctgt
     1621 tccggaagaa aattgtgtaa gtaatactgt cctcgttgcg gctcaaaagt ccatgctgct
     1681 ccaccaaaaa ccgccaacca attattagga ggcgatccgt caggttttgg atctgcccag
     1741 acataccaat ctactttgga attggtgctg tttttccgag attcttgaaa ccacttgtgc
     1801 tgatctgaag agtggttcaa aactaaatcc aaaacaattt tgattccgcg ccgatgagat
     1861 tcttctacca attgatcgaa atcttcgagt gttccgtaat ccgaatcaat gttgcaataa
     1921 tcacttacat cataaccaaa atctgcttgc ggtgatggga aaaatggaga aaaccaaatt
     1981 gcatcaattc ctaaacctcc gcctttgccg tcgttgaggt gatcgagttt ttcaatcact
     2041 cctgctaaat caccgattcc atcattgtta gagtctttga aactgcgagg ataaatttga
     2101 tagaaaacgg ctgattccca ccagttttca gaaagtggac tggatttgag atttaatgat
     2161 ttttgcataa attattgttt tattttattt tttgaagagt aaaaaacgtc tcattatttc
     2221 acagaaccag taaccaatcc gcgcacaaaa taacgttgca aggcgaaaaa gaccagcaaa
     2281 ggaaccacaa tcgaaatgaa ggcactgctc gttaaaattt cccagttgtc gccgcgcgaa
     2341 ccgagcagtt cacgcagctt tgaggtcagt acgatttggt tatcttgttt gccgagaaaa
     2401 acgagtgcca ctaacaagtc gttccaaacc cacaaaaatt gaaaaataca aaatgaagcc
     2461 aatgccggaa tcgataacgg caaaacgagg cgtgtaaaaa tttggaaatg cgatgcaccg
     2521 tcaatgcggg cggattcgat gatgtcacgc ggaagactgc ccatgtaatt ccggagcaaa
     2581 taaatggcga gcggaagccc aaaaccggta tgagccagcc aaattcctaa atacgatttt
     2641 gcttctgtgc cgaaaaatgt accgattttg ttgtacattg tcaacaacgg aattagcgac
     2701 atttgcaaag gtaccaccaa caaacccacc acacaaacaa aaagaaattg tcgtccggga
     2761 aattccatcc atgcaaaagc ataagccgca aatgccgcaa tcattatcgg aataattgtt
     2821 gccggaatgg tgacggtgaa ggtgttgaca aaggattgtc caacattttc ggaagccaaa
     2881 acttcacggt aattgtccaa cgaaaacgag ggcggaacat tggcgacata gaaaatccgt
     2941 ttaccacgtt tgtgagtgaa aggtttttga gaaacccatt cgtactttcc gtctgcttgt
     3001 aaagtaaagt gagaatcgtc tttgaaaatc atcgtttctc cggatttgta ggcggaaagg
     3061 ttccggaagt tggtattgaa agtgagaatt gttttttcac cgggttccag aattgttccg
     3121 gaaatgacat aatttccgga tttttcaatt tgatcttctg cttttccggt tctgccccag
     3181 tcgttttgtt ctgaggtcgt aagtgccgtc caccatccgg agtaggcgag ttgatctttg
     3241 tctcgaaatg aactgacgag caagccgatg gttggcaaaa tccacagcaa aaccagcacc
     3301 agcaaagcca gatggttgaa caatcgtccc agagtaagat gttgtggttt ccggaatgtc
     3361 atcttgcctc ctcttctttc cggaagttac ggatattcca aaacataatc ggaatcacag
     3421 ccatcataat cattaaggcc acaatgcttc cgcgtccgga atctccgccg cctcgaaaca
     3481 tccagtcaaa catcaaattt gctaaaacgt cggtgttcca ttgaccgttg gtcattgcca
     3541 aaacaatgtc gaaaactttg agaacaatga ttgtaatggt tgtccaaacc accataattg
     3601 ttcccataat ttgcggaatc atgattttga aaaatatctg aatttctccg gctccgtcga
     3661 tgcgtgcggc ttccaaagtt gcttccggaa ttccgcgtaa cgccgcactg aaaatgacca
     3721 ttgcaaatcc ggtttgaatc caaaccaaaa tcaccatcag gaaaaaatta ttccaaaacg
     3781 gaagcgtgat ccaggcctgt ggttcaaaac caaacatcat cacaaaagca ttcaaactgc
     3841 cgatttgatc ggtatccggt ccacgataat cataaacgaa tttccaaatc acacttgcac
     3901 cgacaaatga aatcgccatc ggcataaaaa tcaaggattt ggcaatcgtt ccccaccaaa
     3961 gtttgtctgc caaaacggca accgccaaac caaaagcgac gcttaaagtc ggcacaacaa
     4021 tcagccacat aaaattgttg ccgatggcct gcaaaaattc cggatttttc gtcgcccaaa
     4081 tgtaattttt caggccgaca aattcaaatc cgccgaaatt gtaaaagctc aatcgaagcg
     4141 tttctaaaac aggataaacg aggtaaatgg aaagtatcaa aagggcagga gccaaaaaca
     4201 accacggtcg aatggccgct cgctggcgac tgttgcgaac tgcgttttct ccggaatcgg
     4261 caaaaacttg gtcgagcaaa aaattacttc cgacataata aagcgtacac gcaccaacgc
     4321 ctaaaaatac agcgaggagt gcgtttagaa gctgttcaag cattccgaaa aatcggtgga
     4381 gatcggtgat tgagattggg ccgcattgaa atgtgaatgc gacccgttgt gttgggactt
     4441 ttacttaatc gaatcccatg tggactgcac attgtctgca actttctttg cagatgcacc
     4501 gctcacataa taaaccattt gactccaaaa tgcaccggca ccaattgctc ccggcatcag
     4561 gtcggaaccg tcaaaacgga aggtagttgc attttgcaga atttcacctt gcttgcgcaa
     4621 tgtgccgctg gaatatttgc tcagatccac gcccttgtgc ggagtcaaga atccggcacg
     4681 agccatccaa atttcatggg atttaggatg ttgcagatac ttgatgaatt cgcgggtggc
     4741 tttgctgtct ttagccatcg ccaacaatgt tccgccgcct aaaactggat tccccaattt
     4801 ttttgtggag taagacggga agtagaagaa atcagcatct tcgccaacct tcactctttt
     4861 tggaaagaaa gccggaatga aactggcttg acggtgcatg tagcaacgcg gtggcacagt
     4921 gaacaatcct ttggggctgt cacggaaatc ggtcgaaccg actgccgcca ttccgccgtc
     4981 aacatacttg ctgtttttgg caaatttacc aaaagtttca atcgccgcaa ccacacgcgg
     5041 atcgttgaat ttcacgtcgt ttgtcaccca tccgtcataa actgatggcg gttgtgtgcg
     5101 caacatcagg tcttcgaccc agtccgttgc cggccatccg gttgcgtctc cggaaccaag
     5161 tccgatacac cacggagttc cgccgtcttt aacaatttga tcgctcaatg caatcaattc
     5221 ttccattgta ttcggaacag aatatcctcc ggcttcaaag ttttccggag aataccaaac
     5281 caaggatttg acgttcacat taaagaaaaa gccgtaaaac tctttctttc cgcttttgct
     5341 gggataagtt cccaaatcaa cccaggactg tccagcggca taattgcttt tgacccaatt
     5401 tgcggtactg cttcccaaag gaaccaaacc gccgatggct gccatatttg ccgcaagtcc
     5461 cggctgagga aatgccgcca agttcggcgg actacccgcc ttgatgtcaa tgacgatttg
     5521 ctgttcaaaa ctgtcagaac ctgcatattc gactttggcc cctgtggcct tctcgaaata
     5581 tgcaatgact gcttcaaaac tttcttcgtc tgccgtcaac cacggccctg aaatggtgac
     5641 cgtttgccca ctcaggctcg gtcctttgta ctcacctgcc attgcaggca cactaatcag
     5701 tgcggtggca accactgcca gcaacgcacc tttcatggca ttcatgaaat atttcataat
     5761 ctaactcctt ttctttagat taacaattcc ggcatggcaa ccaaccaaac cgtttttaat
     5821 cgttttaact ttttttccaa aacgttttgg aaaaaattta aaacgacaaa tgccgtaaaa
     5881 gagtatgatt atattattta aaagtcaaca aaaaaatact gtctccaact actatttcga
     5941 gagcactggc cggttattca gatgtcagtt ccaagactcg ccgtcgtgtt caggatgcag
     6001 caaaaaaaca taactatcac ccaaacccgg ttgcacaaag acttcaaaaa ggaaaaacag
     6061 agactattgg agtagctctc tcagccagtc aaaattattt taaagacact ttttttcttg
     6121 atttgctggc aggcataagt gttagtctca gtaaagtcgg ttacgattta aacatttcag
     6181 tcgctccaaa ttctgaacac gaaatagatt ctcttcgccg tatggttcag ggaaagcggg
     6241 ttgatggaat aattttgacg ctgacaaaag cagtcgatga acgaatctct tatttgcttg
     6301 atcagaattt tccttttgtt ttgtatggaa gaacagagga accgcgtccg catgcttttc
     6361 tggacattga tggagagctt gcatttcgag aagcctgcac atttctgatt gaattgggac
     6421 acaagagaat agctctgata aatggagaac ccgaatacat gtttccagct ttttgttcag
     6481 agggttacag aaacggatta aagtccggaa accttccttt cgatcctgag ctagtgaggg
     6541 agtggcggca cggagatgca caattgagta gttttcagca aacagtagag ttgatgcagc
     6601 tttcagaacc tcccacagca attgtttttc aaactgaagc cgctgctggt attttacaag
     6661 ccttactaca acttcaatta caagtaggaa aggacattgc cttaattggt tatgatgatc
     6721 tggaaattgc caaaacaaat attccgccct tgactactat gcgtccacct gcacgtaatg
     6781 ccggtgagca gtttgtagga atgctgatgc aaattataga cggacgtgca gcagaagatt
     6841 tacaggaagt ttggaaagca aaaatggtga tgaggcaatc cacaggtcga attagctgta
     6901 atgattctac ttgacagcac tcccatgttt gtggaatcct acacatgaaa aaactaaata
     6961 ttttataatc acatttcttt acacttttta taatcgctaa gacaggagtt ttccatggca
     7021 gaagtaaaaa tgcagtctat ctataagcaa ttcggcaata ccgaagttat tcacggagtc
     7081 gatttggagg ttgatgataa tgagtttgtt gttttagtcg gaccttccgg ctgcggcaaa
     7141 tcaaccctcc tgcgtttgat tgcaggctta gaagacgtga cgagtggaga gattgaaatt
     7201 gatggtgttc gagtcgacta tctaccacca gccaaacgcg gaatagcaat ggtttttcag
     7261 tcatatgcac tttaccctca catgaatgtt taccagaaca tgtcatttgg tttgagactg
     7321 gcaaagaccg acaaacaaat tgttgatcaa aaagtccggg atgcagcaga aatcttacaa
     7381 attaccgaat tgctggaacg aaaaccgaaa gaactttccg gaggacaaag acaaagagtt
     7441 gctatcggca gggcaattgt cagggatcca aaagtttttc ttttcgatga accactctct
     7501 aatctcgatg caaaacttag agtgcagatg cgtattgagt tgacaaagtt gcaccgggaa
     7561 ctcaatgcaa ctatggtata cgtcacacat gatcaggttg aagccatgac tatggcagac
     7621 aaaattgttg tacttagaga gggttatgtt gaacaggttg gtgctccatt agacttgtat
     7681 cataaccctg caaacttgtt tgttgcaggg tttatcggtt ctccggcaat gaattttatg
     7741 gatggaaaga tttcaggaat atctgatcag gcagttgaat ttgaattaaa cagtaagcaa
     7801 aaaattcaaa tcccctgtca accgggagaa caggctgctg cagggagttc aatcaaagcc
     7861 ggtgttcgac cagaacacct tgaattaacc ggagacagtg atgcaatttt acagggagaa
     7921 gtttttgcgg tggagcgtct tggcggggaa acttatcttt atgtacaaac tgaacgaaat
     7981 aatgaactta ccgttcatgc tacgggcgac aagatagtca gcgtaggtga ttctgtatca
     8041 ataggatttg ctccagagtc ctgccatctt tttgacggac agggccaggt ttttgaaaag
     8101 ttggcgccct gatttttcct gttacaacgg gataaactct gcttcggctg cgattgccaa
     8161 ttgagactgg tgatccagca cacgcagttt tgcagcatcg ttaccactta ggtcgccggt
     8221 tagattaaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcacacgc
     8341 agttttgcag catcgttacc acttaggtcg ccggttagat taaaatgatg taaatcgaaa
     8401 accggccttt ttgccctgaa gcggaatttt tttagtttac gccctgattt gcgggcaaca
     8461 aataacgaaa gccacgttgc caataatgga ccatggacta caagccctgg atacccctca
     8521 acttcagtag cataggcccg gtcatagtgg atgcggtgac agttgaaagt caaggcagag
     8581 tagcggaaca gttgtaatgg attcgtgctc atctcttcca tccactcccc agactcttcc
     8641 ggatcattaa gttctcgaat cttcgggtct gaatctgtaa aaacaatatt ccggttttct
     8701 gtggcacaga gagtttcccg aacatggaac tctcgaagga tattaataaa gaccaactca
     8761 ccactgctgc cgttttttcg agtgatatct gtgatgttcc aagttgtttt cacttcatct
     8821 ccaactcgca gcgccctatg aaactcgtat tcagctccgg caaacatccg gcgttttaac
     8881 ggaatgggcg gcatgaatga acctcgcttc ggatgtccat cttctccaag ctgatttggc
     8941 ggaacagaag gttcacctaa ggtcagcaaa aacaacggaa agacagcttc tccagaagat
     9001 ggaggattgc cgttttgagt taatgtgtgg tggagacgtt tggcgtttac ctccgaaatc
     9061 tgttcatgga aaacctccgt tttgcctaac cattcgttgt attcggaatt catttacctt
     9121 tctcaaaatt gttaattttc agaaaattct gcacgaattg ccgcaccgtg ctgatttaga
     9181 ctggggactt ctttgagtga atcttgcggt gcatcgttaa aaatagccgg aggtgccgga
     9241 acattaattt ttccttccgg attggaaatg acggcgcgcc tcagaatcgg atgactggaa
     9301 agcccctgca catcgttgag tgtactgtaa gcaatctccg atttttttag tttggcagaa
     9361 gcctcagtat gagacaattt tccaagagca gtttcaacac aaccgtctac tacggaacgg
     9421 tttgctaagc gagaagaatt gtccggatac tgttccgcaa aatctgcatc aacaagaatt
     9481 tctacacaaa aacgttgcca ttcccgttcg ttttgaatgg acagcaaaat cgtttttccg
     9541 tctgctgttt tgaaggctcc ataaggacaa atcgaggggt gggaaagccc gactctttcc
     9601 ggagcttttc cgccatagtc ttgccacaat aacggaacgg ccatccagtc agccagagca
     9661 tcgaagagcg aaacctcgat gtgctgcccg tgttcgttta tgcctctcgc aatcagtgct
     9721 tccagaattg ctgaccaggc atacattcct gttgcaatat cagccactga aatgccgaca
     9781 cgccccggtg catctgctga tcctgtgatt gaagcaagcc cagtttccgc ctgaatcagc
     9841 aaatcataag ccttcatttt agcccactct ccctgactcc cgtaaccgct gatggagcag
     9901 gtgattagtt ttttgtttgt ctcacgcaac ttgttaaaat caagtcccag tcttttcaaa
     9961 gaacccggct taaagttttc aatcaataca tctgcttttt gaagaatgga acacaaaagt
    10021 tgaatttctt ccggacgttt caaatccatt gaaatcgact cttttcctcg attgagccag
    10081 acaaaatagg cagactgtcc gttgacaaca tggtcataat tacgggcaaa atctccttcc
    10141 ggacgttcaa ttttaagcac tctcgcaccg gcatcagcca atctcgaaga cacaaacgga
    10201 gcggcaaccg cctgctccaa actgacgacc aacaatccgt ccagcgcgcc catcagtatg
    10261 acctcggcag acccagcaca tgctcggcaa tgtaggaaag aatgagattg gtagaaattg
    10321 gcgcaatttg atacagtctg gtttctctga acttgcgctc aacatcatat tcttcggcaa
    10381 atccataacc accgtgagtt tgcaaacagg cttccgcaga tttccaactt gcatcagaag
    10441 ccagtaattt tgccatattt gcttctgctc cggggcgttt tccgtcttca tacaaaagtg
    10501 ttgctttttc agtcatcaag gccgcggcct cagttgccgc ataggcttga gcgagaggaa
    10561 actgaatgcc ctgatttttg ccgatgggac gatcaaaaac gatgcgctcg ttggcataag
    10621 cagttgcttt ttcaataaac cagcgcgaat caccaatgca ctcagacgca atgagaatcc
    10681 gttcagcatt cataccgtca atgatgtagc gaaaaccttt tccttcttcg ccgattaaat
    10741 tttcttgcgg aactcgcaaa tcgtcaaaaa agacttctgt tgtcgcatgg ttgatcattg
    10801 tccggatggg ccgaatcgtt aaaccgtttc cattggcatt gcgcatatcc acaataaaaa
    10861 ccgacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcc tgatcttcgt
    10981 caaacatgaa ttatttttga aagaacttat tagtattaca ttttgtacaa tccctattgc
    11041 atgataaaag gatcggcgat gggatcgggt gaggtgttca gccagactgt tttgggacga
    11101 gtataatcgt aaattgcatc cagtccggac tcgcgtccgt aaccgctgtc tttaaaacca
    11161 ccaaacggag ccaaaggtga aaccatgcga taagtgttga cccagacaat tccggcacgc
    11221 aatgcttttg tcactctcaa tgctctccca atatttgtcg tgaacactcc ggaagccaat
    11281 ccgtagcggg tatcgtttgc taatttaatg gcttctgctt cgtcgcgaaa acgcaacaca
    11341 ctaaccaccg gtccaaacat ttcgcgttga acaattccta aattctgatt aggacaatcg
    11401 acaatggtgg gttcgcaata ccaacccttt tcgagatgag caggttttcc tccgccatga
    11461 atcaaagtgg ctccattggc ggttgcgtct gcgaccgtac tttgaatatt attgagttga
    11521 gcaagtgttg ccaagggtcc catttgactt gtttcggcca atggatctcc aatgcgaatt
    11581 tcttttgctc tcctggcaac ttggtccaga attttatcat ggatactttc atgcagtaaa
    11641 agccgcgaac cagccacaca actctgacca cttgcactga aaatactgag taaaactcca
    11701 ttagtcgcac tttccaaatc agcatcttca aaaacaatca ttggtgattt cccacccaac
    11761 tcaagagaaa cttgcgctaa attctcagca gagttccgga cgacatctcg cgctgtttcc
    11821 ggacctccgg taaagctgac tctagcaaca agtggatggg atgtcaaagc cttggcgcaa
    11881 gggtcaccat atccggtgac gatattcatc actcccgggg ggaaaccggc ttcttcaaaa
    11941 actttggcaa attccagcat cggcgcagat gcatgttcag aggctttcag caccaccgta
    12001 tttccggccg ccaacgctgg gccgattttg acagcaacta aaaacagttg cgagttccaa
    12061 ggcacgactg ccgccacgac tccaagtggc tcgcgaaccg tcattgtcca catgtttggc
    12121 ttgtcaatgg gcaaggtgtc gccatgaacc ttgtccgcaa gtccggcata atattgaaaa
    12181 aaatctgaga tataacgcgc ttgccactgt gtttccttca ataattttcc ggtatctgta
    12241 gtctctgccc gtcccaacga ttctgaatgt tccgccagta cttccgcaag gcgccgcaat
    12301 aatttgcctc gttctgtagc cgtcatgttg gcccatggcc cctcagtaaa agcacggtgc
    12361 gcggctttaa ccgcacgatc cacatcattg gctccagcct ccggaatgat cgcccaaggt
    12421 tcttctgtgg caggattaaa actctcaaag gttttctcac tttcagaatt gacccattgt
    12481 ccatcaatgt acatttggta tttttttaga tctgccatct tgtttctcct tttgtgggtt
    12541 acttaatttt caggtttgta ggccgtgcat tctatttcaa ccttgatgtc gatcaaaaag
    12601 tcgcaaacca aagcggaacg agcgggcgga ccttcgctga agtattctcc gtaaactgaa
    12661 ttaaatcccg gaaaatcgtc acgattcttg agccaaacca tagatttaac aacatcgttt
    12721 aaagtgcatc cggcatcttt gagcgtctcg cgaatcaatt cgatacagac tcgcgtctgt
    12781 tcctcaatag tgccttcggt catgggcgca ccgtctttca ttggcacttg accggtcagg
    12841 aaaataaaat cacctgcgcg cactgctttc gataatgtta attgacgacc attgatgatc
    12901 agtggcccgc caatgatttc tttgccggtt ttcattttgg atctttcttc ttgaactcgt
    12961 ttgttcattt caacacctcc tgaaaaaacg atacaagttg agagttgact gcttgagaat
    13021 tttcggccaa tcccatgtgt ctcaatccgg gaagtattac agcacgagca tccggaatca
    13081 atgctgccat gcgttgcgtc atttccggag aattaccatg atcgtcttct cctgtcataa
    13141 ccaaagttgg gcaacggatg gcactgatgg aatctgtcag tggttcatca cattctgcta
    13201 aaagacgata gatttccgga taaatctctt tgtcatttgc catgatccag caacgtattt
    13261 tatccagaat ttcaggattt tttgctgaaa aatcagcact aaaccagcgt tccaatgcgt
    13321 cttccacagt tgatgcaggg ccgtgttctc cggcttgttg aactcgtgct aataccgcct
    13381 ttctttctga ttttgtgcga ccatgtgctg tattgagcat cactaaagca tttacttttt
    13441 caggatgatt cagagcaaag tcctggacta tcaaacctcc aagcgaaaaa ccaactaagg
    13501 cacaactctc taaattcagt ccatccatca acgctaacaa ttgcccggag aattgactca
    13561 actgacaagg aatctccgga ttcacacttt cgccgtgtcc cagcaaatcg taacaaatca
    13621 cagagaaatg aggcgtaaga ctgtccattt gccactgcca catttgacgg ttcattccca
    13681 agccatgcac caaaatcaca ggcgaaccgg tcccttcttt gctccaggaa gtaccttgaa
    13741 atgaaccgtg ttttatatcg ttcatatcgt catttcggcc taattagcca gcgtgacagt
    13801 accggatttg aaatgcggca gcacttcttc gttgaacagc ttgatactgc gcattgcagt
    13861 ctcatgtgga acatccggga aatgagacca aagcatcaac gactgaagat tcaattctga
    13921 ctgtaactct tttatctttt caacaatgta ttccggagtt ccaaacagca tgttacgcgg
    13981 gtgtaaccac tcataggtca gagcatccag tttaccctga gtgtccggaa gttcttctcc
    14041 ggggtcaaga tgattaccca agccgcgcca atgacaaatc catttgagat atttgagaat
    14101 gccgtccccg cccaaacgct gagcatcttc catattttct gcgcagaaca tatcgcgaac
    14161 caatgaaatg ccttcgccta aaggtacttc ccgccccaac ttttccgagc gtgcatctcg
    14221 atatatttca aagcgttttt tcaaagtttt cacagttgga atccacataa tcacattgat
    14281 gtcgttcttt gctgcccact caatggagct tgtggaatcg actacctgcc aaagttgtgg
    14341 atgaggcttt tgcaacggtt ttggcaatat ggaaattttc tcgatttcat tggtttccat
    14401 gttcatgaag gtttcagatt tcggactgtc cggatggctc caaatcagtc cgggctccga
    14461 gtaagtgaaa aattcacctt gatgagaaat gaaatcctgt gtccaggctt tcttcatgag
    14521 ttcaacggtt tcagcaaata aacggaaatt ctttgcctga tcttttaagt ccgcatcttt
    14581 gttcaaattt gaggcttccc ttccataaac accacgacca atccctactt caactcggcc
    14641 attgctcatg tgatccagca cagcgatatc ctcaacgaaa cgtaaaggat tccagaaagt
    14701 aacaatgttt gccgcctgac cgagacggat gcgagaggtg cgggccgcaa tgtctgtaca
    14761 caacattatt gcgttgggac aaacttcaaa accttcatga ccgaagtggt gttcggtgaa
    14821 ccaaatcgaa tcccaacctg catcttcaat tgtcacagcg aggtctcgaa cttcgcctaa
    14881 aatttgacca taaggtttta ccagtccggg gttgctaaca ttcgcaaaat atccaaaatc
    14941 cataatttta tcagaaagtt aacttttatt tttatattta gtgagaattt gaaaacatca
    15001 atatttcaag attcgagctt caataattta ttccattccg gaaacaagtc aggaatcaac
    15061 ttccgaacct gaggctttca gagcattgcg gatggcgacg aggccgcggt ctcgatcacg
    15121 attcatctca gagatttctc ggccatcagc ttcacgcaga catccatcaa ccattttatt
    15181 acgcagttcc ttagtcagtt caggagcttt cagacgagtc cagggaagtt tcaaagcagg
    15241 accgaattga tcaagagtgt aagccatgcc accttctccg ccaccaagat ggaaagccat
    15301 acaaggcccc atcaaggccc aacgcggtcc aggtccattt gtgatggcaa agtcgatttg
    15361 ctcaaccgtt gcttcatcgt tgtccaccat gtgcagggct tcgcgccaga ttgcttcctg
    15421 caaacgagtt gcaatgaaac ctgggatttc tttgctcaaa actaaaggag ccttgcctgc
    15481 aacttgataa aactcaacca accattttac cgcctccgct gatgtttgac tgcctctgac
    15541 aatttcgacc aacggaagca aataaggagg attgaaaggg tgcccaacta ccgttcgttg
    15601 aggtgtggaa cagtgggctt gaatttcgct catgggcaag cccgaagtgc tggaagcaat
    15661 aacaacattt tctggaacaa gttcaccaag ttgtgcaaaa atcttttgct taagagcaag
    15721 attctccggc acactttcct gaatgaattc cgaaccgtta actgcgtccg caagatcggt
    15781 gctgtaataa agattgtctc gtgatgctcc ttccgcgagt cccaatgctt ccaaactgat
    15841 ccatgcagta tttattaaat tatgtaatac atttttctcc gattcctcat gcaaatagac
    15901 agagacctta tagccctgtg agagaaaata tgctgcccac cctgctccaa tgggacctgc
    15961 gccaatgctg gtaacttttt gcactgactt tggatctttt cctgaaaatt cttgtgtcat
    16021 aatgtttttc ttgttaataa aatagacaat tcataaaccc ttaaaatccg gtttacgttt
    16081 ttccacaaat gcttttaccc cttctttggc atcatctgat cctaacattt ttctataaac
    16141 aggcaaatct gcttttcgca ttgtttgaaa agactgttca atggtatcgc cttcgatggc
    16201 acgcaaaact tccttgacag tctgcaaagc aagcggggct gattcggcaa tttgattggc
    16261 ccattctctg gccgtttcca tcagtatctc cttggagaca accttattca caaaaccata
    16321 tccttgtgcc tcgctggccg tcatccgtcg accgaggagg tacatttcca ttgcgacatt
    16381 atacggaatc cgtctaggaa ggcgctgaat ggcacctgca tcagggacga tccctaatgg
    16441 cagttccggt aacccaaatt ccacatgttc ggcagcgatg attaaatcac aggccattgc
    16501 catttcaaat ccaccaccaa ttgccaagcc gttgagagcc acaatcacgg gtttattcaa
    16561 atcccacatt tcggtcaatc ctgcaaaacc tccggctcca tagtcgtttt cccaccaatc
    16621 atctaaggac atttctccct gattcagtga cttgagatcc caaccggcag aaaaaattct
    16681 atcccctgcg gctgttagga ttcccaccca cagttccgga tcatcacgca acaaaacaaa
    16741 agcatcccct aatttccggc tggtagcaag atcaattgca ttgacttttg gcctgttcag
    16801 gagaatttca agaactctcc ctttcttaac aacggttact tcttccatat aatattccct
    16861 ttcaattaag aaattttttt aatctttttt aattacctgt tttatttgtt gttaactgca
    16921 cctgacgttc cggttgcaat acgcatcaag acatcaaccg cacacacctt ctccttataa
    16981 acgaatagcg gattgatttc aatttctttg ataccgattg ctgggttcat gactcccacc
    17041 acaaggttaa gaagtgccgt aacgagttgt tctgtgtcaa tcatttcgcc acctcggaaa
    17101 ccatcaaaaa gtttcgatat tttcaaactc tttagcgcat tttccaagtc attgcgattc
    17161 gtcggtagta gaattgtaac tgcgtcggca agcagttctg tcagaattcc accagcggcc
    17221 agtgtcatgg ccattccgaa ctgaggatca gttctaacat taaccaacaa ttcagcaatg
    17281 ggtctctcaa gcatcttttc aacaagaaat ttctccgaaa ccgcagccgg atcaaaatgt
    17341 tctacatcct ttttcatgcg gtcaacagcg attttgacct cctcgcaact caaaagattc
    17401 aaagctattg cacctgcatc ggtcttatgt gccagatttt taccgaccat tttgacaaca
    17461 accggaaagc ctatctt
//
LOCUS       DTVD01000234            4872 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5953_length_4872_cov_355.655180, whole genome
            shotgun sequence.
ACCESSION   DTVD01000234 DTVD01000000
VERSION     DTVD01000234.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5953_length_4872_cov_355.655180"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            241..2118
                     /locus_tag="EYO68_08485"
     CDS             241..2118
                     /locus_tag="EYO68_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIC07880.1"
                     /translation="MSSVEAPLRAKSVSSQVNIPKTELEKASIRFAGDSGDGMQLTGT
                     RFSATSVMAGNDVITFPDYPAEIRAPAGTLAGVSSFDLSISSNEIYTTADFLSVLVVM
                     NPAALKMNLQDLEKGGILIINSDSFEENDLRKAEYSENPLESGELNDYRVVQIPITKL
                     TLRAVEETGLSHRDGVRCKNFFSLGVVQWLFVRSLDQTRDWIEDKFKNKPEIAKANVL
                     ALQAGYNYAITTELFQARYNVPPAVLPSGEYRQITGNQAFSLGCVAASLRSGLSLLYS
                     SYPITPASDILHELAQYKNFGVKTIQAEDEIAAMSSSIGSAFGGVLAVTGTSGPGLDL
                     KSEAIGYAVMAELPVVVLNVQRGGPSTGLPTKAEQTDLLAAMYGRHGEAPVPVLAPAT
                     PGDCFYMVLEAFRLALKYMTPVIVLSDGGLANASEPWIIPDVDQLPDLTSNIHTDPEN
                     FSPSNRNPETLGRNWAIPGTPGLEHRLGGLEKDIVTGEINYEPENHEKMVLLRDEKIR
                     RIVKDLPPLELIGPEKNELLVVTWGSVFGPAFSVINELQTEGYPVSMLHLRHLNPFQE
                     KLGEVLRNFKKVLVPEMNLGQLSRLLRAEYLVDAISFSKLQGRPFLISEIRNRVLEFF
                     D"
     gene            2186..3214
                     /locus_tag="EYO68_08490"
     CDS             2186..3214
                     /locus_tag="EYO68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIC07881.1"
                     /translation="MNIPVKPVSTVELSKADFVSKQTVRWCPGCGDYSILANVQKVMP
                     ELNIPRENIVFVSGIGCSSRFPYYMNTYGLHTIHGRAPAFATGLKVARPDLSVWIVTG
                     DGDGLSIGGNQLLHMLRRNLDVNVLLFNNRIYGLTKGQYSPTSEQGKVTKSTPFGSPD
                     MPLNPMLFALSAGATFIARTFDVDAKGMREIFKEAAKHKGTSFVEIYQNCNIFNDGTF
                     SAIYERSVREDRILRLKHGKPFIFGKEQNKGIRLNGLKPEVVDLRDGVNENELLVHDQ
                     YSRDRILANLLSGFDYPDFPVPMGIFRQVENSTYEEKIEEQIKSQIEKKGVGNLEKLL
                     RGNHAWTN"
     gene            3284..3359
                     /locus_tag="EYO68_08495"
     tRNA            3284..3359
                     /locus_tag="EYO68_08495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:3317..3319,aa:Val,seq:tac)
     gene            3375..3451
                     /locus_tag="EYO68_08500"
     tRNA            3375..3451
                     /locus_tag="EYO68_08500"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:3409..3411,aa:Asp,seq:gtc)
     gene            complement(3596..4813)
                     /locus_tag="EYO68_08505"
     CDS             complement(3596..4813)
                     /locus_tag="EYO68_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008281556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC07882.1"
                     /translation="MDLPEETEVAVIGSGYTGLCTAIQTSRNGLSTVVLDAQDAGWGG
                     SSRNGGQVSTSLKPSFKELSHKYGEEQARELLKEGINALKWIGDFIQEEKIDCDFKRT
                     GRFYGAHSLSQFKQLEKKIREQPEGLQMDVNLVSKSQQHTEIGSDFYHGGVVYPYHAS
                     LDPAKFHQELLERALSSGVQIKTLCAVNKIVKKRGIFQLQTEAGTMTAREVVVATNGY
                     TGTVTPWLKRRIIPIGSYIIATETLEEGLVDELIPKDRVITDTRKLVVYFRASPDRKR
                     ILFGGRASLNETDPEKCVKPLYKQLTKIFPQLTKTKVSHSWMGFVGFTFDHMPHTGNQ
                     DGVHYAMGYCGSGVSLSSYFGTKLGQRLAGLPEGNTVLTDIKFQTRPFYSGNPWFLAP
                     SILYYRLLDRYLS"
BASE COUNT         1320 a         1017 c         1086 g         1449 t
ORIGIN      
        1 tggtgtgtct ttaccttcga taattcgagc ggaagtttta ctgcaagcga taatatgagc
       61 gcccaatcga cacttgcaaa cattcaataa tattgtattt gccttgaaac aaaatttatt
      121 ttgaaacact ttcgcttctc atttgcagtt caggaagttt tttgttaaaa ttaagtaact
      181 tgtttctgtc ttctttattt attttcacaa acagtttcac caaaattttc aggaccaaaa
      241 atgtcttcag tggaggcacc tttacgcgct aaatcagtat catctcaagt caacattccc
      301 aaaacagaac tagaaaaagc ctccatacga tttgctggag attcaggtga cggaatgcag
      361 ctgactggaa ccaggttttc tgcaacttca gtcatggcgg gaaacgacgt tattactttt
      421 cctgattatc ccgctgagat tcgtgctcct gcaggaacat tggcaggtgt gtcaagtttt
      481 gatctcagta tttccagcaa tgaaatctat accactgcag attttctgag tgttttggta
      541 gtaatgaatc ctgcagctct caagatgaat ttgcaggatt tggaaaaagg aggaatccta
      601 attatcaatt cagacagttt tgaagaaaat gatttgcgaa aggctgaata ttctgagaat
      661 cctctggaga gcggagaact gaatgattat cgtgtagtgc aaattccgat tacaaagctc
      721 actctgagag ctgttgagga aaccggtctt tcccatcgtg atggagtccg ctgcaaaaac
      781 ttcttttcgc ttggagttgt ccaatggctg tttgtcaggt ctttagacca gaccagggat
      841 tggattgagg acaagttcaa aaacaaacct gaaatagcga aagccaatgt acttgcgctg
      901 caggcgggat acaactatgc aatcacaacc gagttatttc aggcacgtta taatgttcca
      961 cctgcagttt tgccttccgg ggagtatcga caaatcaccg ggaaccaggc attttcatta
     1021 ggttgcgtag ccgcctcttt acgttccggg ctttccctgc tatattcaag ttatccgatc
     1081 acgcctgctt ctgacattct tcacgagttg gcacagtaca aaaattttgg cgtaaaaacc
     1141 atacaggcag aagacgaaat cgcggcaatg tcttcctcca tcggttctgc atttggcggt
     1201 gttttagcag taaccggaac cagcggtccg gggttggatt tgaaaagtga ggctatcggt
     1261 tatgccgtga tggcagaact gcctgtggtg gtactgaatg tccagcgcgg cggaccttca
     1321 accggattac cgacaaaagc ggaacaaaca gatttactcg cagcaatgta cggccgccat
     1381 ggtgaagctc ctgttccggt gcttgctcct gcaactcccg gcgattgttt ttacatggtg
     1441 cttgaagcct ttcgccttgc tctgaaatat atgactcccg tgattgtgct cagtgacgga
     1501 gggctggcca atgcgtcgga accgtggata attccggatg tcgatcaatt accggacctc
     1561 acgtcgaata ttcacactga tccggaaaac ttttctcctt ctaaccgcaa tccggaaact
     1621 ctgggacgca actgggccat ccctggaaca ccaggcctgg aacatcgact tggtggtttg
     1681 gaaaaagaca ttgttactgg agaaatcaat tatgaaccgg aaaaccatga aaaaatggtt
     1741 ctgctgcgtg atgaaaaaat tcggcgtata gtcaaagatt taccgccgtt ggaattaatt
     1801 gggcctgaaa aaaatgagct tctggtagtc acctggggca gtgtttttgg tccggcattt
     1861 tccgttatta atgagttgca aacagaagga tatccggttt ccatgctgca tctcagacac
     1921 ctgaatcctt ttcaggaaaa gttgggagag gtgctccgga actttaaaaa ggtattggta
     1981 ccggaaatga atttagggca attgtcacgc ctgctgcgag cagaatattt ggtggatgca
     2041 atttcttttt cgaaactgca gggccgtcct tttttaattt cagaaatccg taatcgagtt
     2101 cttgaatttt ttgattaaga cgtaattacc tgcctttgaa ggaatcttta tagattgtta
     2161 attaataatt atcttaaaaa acattatgaa tataccagta aaacctgtta gtactgtaga
     2221 acttagcaag gcagattttg tttcaaaaca aaccgtacgc tggtgtcccg gctgtggaga
     2281 ttattcaatt ctggccaatg ttcaaaaagt gatgcccgaa ctgaatatac ccagagaaaa
     2341 tatcgttttt gtttccggaa taggctgctc cagccgcttc ccatattata tgaatactta
     2401 tggattgcat accattcatg gacgggcgcc tgcattcgcg acgggactta aggtggcacg
     2461 tccggatctt tcggtgtgga ttgtcactgg agacggagac gggctgagca tcggtgggaa
     2521 tcaactgctg cacatgcttc ggcgcaacct tgatgtcaat gttttgttgt tcaataatcg
     2581 gatctacggc ctgacgaaag ggcaatattc accgacttct gagcagggta aagttactaa
     2641 aagtacaccc ttcggctcac cggatatgcc gctaaatcca atgcttttcg ctctctctgc
     2701 cggtgcaact ttcattgcac gtacctttga tgtggacgca aaaggaatgc gggaaatctt
     2761 taaagaggct gcaaaacaca aaggtacttc gtttgttgaa atctatcaga attgcaatat
     2821 atttaacgat ggtacttttt cagctatcta tgagcgttct gtacgtgaag atcgtatttt
     2881 gcgtctcaaa catggaaagc cttttatctt tggaaaagaa cagaacaagg gaattcgctt
     2941 gaatggattg aagcccgaag ttgtggattt gagagatgga gtcaatgaga atgaattgct
     3001 ggttcacgat caatacagca gggacagaat actggcaaat ttattaagtg gctttgatta
     3061 tcctgatttt cctgtgccta tgggaatttt tcgtcaagtt gagaattcta cttacgaaga
     3121 gaaaatagaa gaacaaatca aatcgcaaat agaaaagaag ggtgttggaa atttggaaaa
     3181 acttcttcgc gggaatcatg cctggacaaa ctgaattaat ttctaagaat taaaaaacaa
     3241 cttgcactcc gtttggttat taaatagcat tgtaagtttg gttgggcgat tagctcagct
     3301 gggagagcaa cggccttaca agccgtaggt cacaagttcg aaccttgtat cgcccaccat
     3361 tatacccgga taccggggtc gtagttcagt tggttagaac gccggcctgt cacgccggag
     3421 gccgcgagtt caagtctcgt cgaccccgcc agcttttctg gcattgaata gaaaggtttt
     3481 ctcaggagaa cctttctttc atccttcctt ttgtcttaac gaagataatt gcccactaga
     3541 ttatttattt ttgaagctga ttggcgttct tgaatccctt ccaagtttaa ggaaatcaac
     3601 ttaagtagcg atcaagtaat cggtaatata gaatagaagg ggccagaaac catggattac
     3661 ctgagtagaa tggacgggtt tgaaacttga tatctgtcaa cacagtattt ccttcaggga
     3721 gtcctgccag cctttgacca agttttgtcc caaagtaact tgacagagaa actcctgatc
     3781 cacagtatcc catggcatag tgaaccccat cctgatttcc ggtatgtggc atatgatcaa
     3841 atgtaaagcc gacgaagccc atccaagagt gactgacctt tgtctttgtg agttgaggaa
     3901 atatttttgt gagttgtttg tacagaggtt ttacacattt ttctggatca gtctcattta
     3961 gagatgctct tccaccaaac aaaattcgct tccggtcggg agacgctctg aaataaacta
     4021 ccagttttct ggtatctgta ataaccctat cctttggtat caattcgtct actaagcctt
     4081 cctctaatgt ttcagtggca attatgtaac tgccaatagg gattattctt cttttgagcc
     4141 aaggagtaac tgttcccgta tatccgtttg tggcgacaac aacttcacgg gcagtcattg
     4201 ttccggcttc tgtttgaagc tgaaaaattc cccttttttt cactattttg tttaccgcac
     4261 aaagagtctt aatctgcacg ccactggaga gggctctttc aagtaattct tgatggaatt
     4321 ttgcaggatc aagagaagca tgataggggt aaacaacccc cccatggtag aaatcactgc
     4381 ctatttctgt atgctgctga gattttgata caagatttac atccatctgc agaccttctg
     4441 gttgttctct gatttttttt tctaactgct taaattgaga tagagaatga gcaccataga
     4501 aacgtccggt acgtttaaaa tcacaatcaa ttttttcttc ctgaataaaa tctccaatcc
     4561 atttcagggc gtttatacct tctttcaaaa gttcacgtgc ttgttcttca ccatacttgt
     4621 gggaaagctc tttaaagctg ggtttgaggc tggtgctgac ttggccgcca ttacgggaac
     4681 tacctcccca gcctgcatcc tgagcatcta aaacaactgt acttagacca tttctgctgg
     4741 tttggatggc tgtgcataat cctgtatatc ctgatccaat gactgcgact tcggtttctt
     4801 ctggcaggtc aatttctttg ataatgggcc ggggcgtttt ttcccaccag taaggagtgt
     4861 ttttgtagtt tg
//
LOCUS       DTVD01000235            4864 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5969_length_4864_cov_329.338324, whole genome
            shotgun sequence.
ACCESSION   DTVD01000235 DTVD01000000
VERSION     DTVD01000235.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5969_length_4864_cov_329.338324"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="EYO68_08510"
     CDS             complement(<1..187)
                     /locus_tag="EYO68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HIC07883.1"
                     /translation="MSKKYLLGVDGGTEGIRAGIFDISGTPLAFASTSYPTQFPAPSW
                     AEQDPNDWWDALGKSVRK"
     gene            complement(195..983)
                     /locus_tag="EYO68_08515"
     CDS             complement(195..983)
                     /locus_tag="EYO68_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC07884.1"
                     /translation="MDLGLRDKIVLVTGGAKGIGAATVKAFLAEDSKVVLVDRDADEG
                     SALSKSSGSKVSFIEADLTNLKSCKEAVEQTVSLFGGLDVLVNNAGFNDGLGLETSPE
                     DFMTSVQNNLLHVYAMTHYSLPQLRKGPGSILNLGSKVSVTGQGQTSAYAAAKGAISA
                     LTREWAVALAPDKIRVNCVIPAECLTDQYEQFFQSQTDPDAAKKAIADLVPFAQRLTT
                     PEEIAQTIVFLASECSSHTTGQLVYVDGGYTHFDRAVGHDHNKW"
     gene            complement(1022..1483)
                     /locus_tag="EYO68_08520"
     CDS             complement(1022..1483)
                     /locus_tag="EYO68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter"
                     /protein_id="HIC07885.1"
                     /translation="MLKNLDPLLNPNLLYILRAMGHGDVLTIVDSNFPADSVASTTVH
                     GEVIRLDGAGIPEVASAIFSVFPLDSFIEAPIQYMQVVGEPETMLEVHKDLHKIALEN
                     ADREWKMDSVERHSFYEQSRKTYAVIATAERRPYGCFMITKGVIGPDGKVM"
     gene            complement(1582..2478)
                     /locus_tag="EYO68_08525"
     CDS             complement(1582..2478)
                     /locus_tag="EYO68_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIC07886.1"
                     /translation="MLDIIDTHQHLWNLDRLKLPWVEGVPALNRSFEISDYLKASASS
                     GIRRTVYMEVDAHPDDKQKEIDDLTLHCKADSDVMLGMVVSADPCKAGFTEFLDANSG
                     NPFIKGVRQVLHNPEIPPKYCLTPEFVRGIRELGKRKLLFDICIRPAELHDAVELCSQ
                     VPETTFVLDHCGNADPNVVNGQREIDSSSSDTTFLHSKESWQKGIFKLGELENVYCKI
                     SGIVARAEAGWNTDTLAPTVNACLDAFGVDRVVFGGDWPVCTLGSSLAQWIAVLKEIV
                     ANRPAEMQEKLFSLNAERLYQL"
     gene            complement(2682..3602)
                     /locus_tag="EYO68_08530"
     CDS             complement(2682..3602)
                     /locus_tag="EYO68_08530"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07887.1"
                     /translation="MREIQVVFGIDMETDIGSWTPFYEGVTKGTPLLLNLFAKKSIPV
                     TAFWVADTARRFPEIVREMESAGHETGTHSLYHETVGDSLFEIPGTYPLLPEEVQPRI
                     KLATDILEDIVGHKVVSWRCPRLFGGTHVTNALEALGYECDATYPMYFYNEQLFPYHP
                     SQENWTEQGSLNLIEIIQFADMGMESKDDYGRDKDQWPLYRTQSTEAFIPHIESFMSY
                     LEKNDQSQKPIVLCFYFHPWEFWEMPEGVIHFGEGGVLPDPFLTKGCGKYCLNQVELL
                     IDWLKSKEAAFLTAGQCARKWHEILALQEI"
     gene            3764..4780
                     /locus_tag="EYO68_08535"
     CDS             3764..4780
                     /locus_tag="EYO68_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIC07888.1"
                     /translation="MNPFETRLLGNTGIKIPSLGFGGAPLGELFEKVEHLQAIETLDE
                     AYASGIRYYDTAPWYGHGLSEHRLGNLLHQKNRHEFILSTKVGRVYRPFKGDPVLFDG
                     SPWVGGLPFEWNFDYSAAGFERSFLDSTMRLGLNQIDLLVIHDLDRSYHGKSVSNKLQ
                     ELETGMEWLKMMKNSAAIQAFGAGINDIEMIPLFLEHFELDFFLVAMPYTLLNQEPLE
                     DIFPECERRGIGIIIGSPYASGILASGSKEESKYGYAAASEEMRKKVQSIEVICEAHG
                     IPLKAAALQFPLAHPQVSSVIPGALRAAQVNENLEMLKIHIPLEFWVELKQTGLLHPE
                     APVA"
BASE COUNT         1326 a         1108 c         1024 g         1406 t
ORIGIN      
        1 ctttcctgac tgacttgccg agagcatccc accagtcgtt ggggtcctgc tcggcccagc
       61 tcggagcagg aaactgagtt ggatatgacg ttgaggcgaa ggcaagtgga gttccggaaa
      121 tatcaaatat tcctgcacgg attccttctg tacccccatc cactcccagt aaatattttt
      181 tactcattca attcttacca tttgttgtga tcatgtccta ccgccctgtc aaaatgtgta
      241 tatcctccat cgacgtagac gagctgtcct gttgtatgag aagaacactc ggaagccaga
      301 aagacaattg tttgcgcgat ttcttcaggt gtcgttagcc tttgtgcaaa cggtacgagg
      361 tcagcgatgg cttttttggc agcatccgga tcagtttggg attggaaaaa ctgctcgtat
      421 tgatctgtaa gacattctgc aggaattaca caattgacac gaattttatc tggagccaaa
      481 gctaccgccc attcgcgtgt aagcgcactg attgcaccct ttgcggcggc ataagctgaa
      541 gtttggcctt gtcccgtaac agaaactttt gagccgagat taaggataga tcctggccct
      601 ttgcggagtt ggggcaatga ataatgcgtc attgcataaa catgcagaag attgttttga
      661 acagaagtca taaaatcttc tggagaagtt tcaagtccca gaccgtcatt gaatccagca
      721 ttatttacca gcacgtccag tccaccgaac aatgaaacgg tttgttccac ggcttcttta
      781 catgatttaa gatttgtaag gtcggcttca ataaaactaa cttttgaacc tgaagactta
      841 gataatgcag agccttcatc tgcatcccga tcaaccaaga ccactttgga gtcttctgcc
      901 aaaaaagctt taactgtagc tgcaccgatt ccctttgcac cccctgtaac aagtacaatt
      961 ttgtctctca gtcccaagtc catgtgttat ttttctttgt tgcagggtga atatattttc
     1021 tctacattac tttcccatcc ggtccaatta cgccttttgt gatcatgaaa caaccgtatg
     1081 gccgacgttc tgcggtagct atcacagcgt aagtttttct agattgctcg taaaaactat
     1141 gcctttctac tgaatccatt ttccattctc gatctgcatt ttccaaagcg attttatgta
     1201 aatctttgtg tacttcaagc atggtttccg gttccccaac cacctgcata tactgaatag
     1261 gtgcttcaat gaaactgtcc agtggaaaaa ccgaaaaaat tgcactggca acttccggaa
     1321 ttccggcgcc atccagccgg atcacttcac catgaacagt tgtcgaagcc actgaatctg
     1381 ccgggaaatt gctgtctacg atggtcagaa cgtctccatg tcccattgca cggaggatgt
     1441 aaagcaagtt tggattaagt agaggatcaa gattttttaa cataataatt ccaatgtttc
     1501 aggtttaagg atcaatttat tagccgctaa gaacactaag agacactaag tttttttctt
     1561 ggtgatctta gcggctatat tctatagctg gtagagtcgt tcagcgttta agctaaacag
     1621 tttttcctgc atttctgcag gacgattggc gacaatttcc ttgagtaccg ctatccattg
     1681 tgccaagctc gaaccaagag tacaaaccgg ccaatcacca ccaaacacta ctcggtctac
     1741 tccaaaggca tcaaggcagg cattgactgt cggagccaga gtgtctgtat tccatccggc
     1801 ttcggcacga gccactattc cggaaatttt gcagtacacg ttttcaagtt ctcccaattt
     1861 gaaaattcct ttttgccaac tttctttgga gtgtagaaaa gtcgtatccg aactggatga
     1921 gtcgatttcc cgttgcccgt ttacaacatt cggatcagca tttccgcaat ggtccagaac
     1981 aaaagttgtt tccggaacct gactgcaaag ctcaaccgca tcatgtagtt ccgcaggacg
     2041 tatgcagatg tcgaagagca gtttcctctt ccccaactca cggatgcccc tcacaaattc
     2101 cggagtgagg cagtatttcg gtggtatttc aggattgtga agaacctgac gcacaccttt
     2161 gataaaggga ttgccggaat tagcatccag aaattcagta aaacctgctt tacacggatc
     2221 agctgaaacg accattccaa gcattacatc cgaatctgcc ttacaatgca gagtcaggtc
     2281 gtcaatctct ttctgcttgt catccggatg tgcatccacc tccatgtaaa cagttcgtct
     2341 gattccggaa cttgctgatg ctttgagata atccgaaatt tcgaaactgc ggttaagtgc
     2401 cggaacccct tccacccagg gtaattttag acgatccaga ttccaaagat gctgatgtgt
     2461 gtcaattatg tcgagcattt taggggtgaa aattcaaatt tttagacttc attgttcttc
     2521 ggatgtacaa gctagcacac gataccaaag agatcaatcg aattaattga tacaattaca
     2581 agaaatataa acgactaatt ggcaggactt ttaagtggcc tccacttcaa ggacaatttc
     2641 tcttttgaat taaggtgtat ttttgtttgt cttacagttc tttagatttc ctgtagggca
     2701 agtatttcat gccatttgcg ggcgcattgg cctgctgtga ggaacgcggc ctctttggac
     2761 ttcagccagt ctatcagcag ctcaacctga ttcaggcaat acttaccgca acctttggtc
     2821 aggaacggat ccggcaacac tccaccttct ccaaaatgaa tcacgccctc aggcatttcc
     2881 caaaactccc atggatgaaa ataaaagcat aagacaatcg gtttctgact ctgatcattc
     2941 ttttccagat aactcataaa ggattcaatg tgcggaataa aagcttctgt agactgtgtg
     3001 cggtagagag gccattggtc tttatcgcga ccgtaatcgt cctttgattc catccccatg
     3061 tcagcaaatt ggataatttc aatcaaattt aaactcccct gttccgtcca gttttcttga
     3121 ctgggatgat aagggaaaag ctgctcattg taaaaataca tggggtaagt ggcatcacat
     3181 tcataaccca gggcctccag ggcgtttgtg acatgagttc ctccaaaaag tctcgggcag
     3241 cgccaggaaa caactttgtg accaacaata tcttccagta tatctgtggc aagcttaatt
     3301 ctcggctgca cttcctccgg taaaagagga taagttcccg gaatttcaaa caaactgtcg
     3361 cccacagtct catgatagag tgagtgagtt cctgtttcat gacccgcgga ttccatttcc
     3421 cgcacaattt ctggaaagcg cctggctgtg tctgcaaccc agaaagctgt cacgggaatg
     3481 gattttttgg caaacagatt cagcaacagc ggagtcccct tagtgactcc ttcataaaaa
     3541 ggagtccaac tgccgatatc agtttccata tcaataccaa atactacctg tatctctctc
     3601 atcctgcata cccatttatg acaaaatgta atttgtggat tttatctttc aacacaattt
     3661 tactatactt agtgtcagga aagctaaaaa tttattacat aatttaggga ctccatttaa
     3721 gtaatgaata gtccccttcc aaaacttcca gagacagact gaaatgaacc cttttgaaac
     3781 caggcttctg ggtaatactg gaattaaaat accttcactg ggattcggtg gtgctccctt
     3841 gggagaattg tttgaaaagg ttgagcatct gcaagcaatt gaaacactcg atgaagctta
     3901 tgcttcagga attcgttact acgatactgc accttggtat gggcatggct tgagtgaaca
     3961 tagattgggc aatttgctgc accaaaaaaa ccgtcatgag tttatccttt ccaccaaagt
     4021 aggaagagtg tatagacctt tcaaaggtga tccggttctc tttgacggat caccatgggt
     4081 cggaggtctt cctttcgaat ggaattttga ttattccgcg gcaggatttg aacgttcatt
     4141 tcttgacagt acaatgcggc tcgggttgaa tcagattgac cttcttgtca ttcatgattt
     4201 ggacagaagt taccatggta aatcagtttc caataaactt caggaactgg agaccggaat
     4261 ggaatggcta aaaatgatga agaattcagc agctattcag gcctttggag caggtatcaa
     4321 tgacattgaa atgattccgc tttttttaga acatttcgaa ctggattttt tcctggttgc
     4381 catgccttac accctgctga atcaggagcc gcttgaagat atatttccag agtgtgaaag
     4441 gcgtggtatt gggatcatta ttggttcacc ttatgcgtca gggatattgg ctagcggctc
     4501 caaagaagaa tccaaatacg gatatgccgc tgccagtgag gagatgcgga aaaaggttca
     4561 gtctatagaa gtgatctgtg aagcgcatgg aattcctctt aaagcggccg cattacagtt
     4621 tccattggct caccctcaag tttcgtcagt catacccggt gcattgagag ctgctcaagt
     4681 taatgagaat cttgaaatgc tcaaaattca tatacctctt gagttctggg ttgaactcaa
     4741 acagacaggc ttgctgcatc ctgaagcgcc ggtagcttga tctttctgcc attggattaa
     4801 tcagtgtgtg agacatacat ttcattaacc ccaacaccat gctaagtatt tgctgtcaaa
     4861 agta
//
LOCUS       DTVD01000236            4855 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_5989_length_4855_cov_193.669167, whole genome
            shotgun sequence.
ACCESSION   DTVD01000236 DTVD01000000
VERSION     DTVD01000236.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5989_length_4855_cov_193.669167"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="EYO68_08540"
     CDS             complement(<1..208)
                     /locus_tag="EYO68_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIC07889.1"
                     /translation="MSIVEIKIPSPGESITEVEIGNWLKADGDYVLIDETICEIETDK
                     ATLPLIAESAGRLKILLQEGETAGI"
     gene            complement(337..3108)
                     /locus_tag="EYO68_08545"
     CDS             complement(337..3108)
                     /locus_tag="EYO68_08545"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIC07890.1"
                     /translation="MNPLSYLNNADIGAFEGLYQQYQQDPNSIDQEWRNFFEGFEFSK
                     ADFSQEAQAKPVVDHTEEVVPEQFQKEMAVSNLIGAYRQRGHMFANTNPVRPRRIHEG
                     EIVLESFGLSEADMDTEFHAGTRVGIGNATLREIYELLEQTYCGSIGVEYKFVRTIEI
                     INWLEQKMESCRNTPNFSREEKIELLRKTNEAVAFESFLHTKFVGQKRFSLEGGESII
                     PALDMILEYGAELGVEEFVIGMAHRGRLNVLANILGKTYSDIFAEFEGKAFGSDGFSG
                     DVKYHMGYSSDKKVRSGKKVHLSLTPNPSHLEAVNPVVEGISRAKIDQYHEGNVKKLV
                     PILIHGDHSMAGQGIIYEVLQMSKLPGYETGGTVHLVINNQVGFTADFMEGRSSTYCT
                     DVAKTTLSPVFHVNADDIEAVAYVTKLALEFRQEFHRDVFVDILGYRRHGHNEADEPR
                     FTQPDLYRRIARHPKVREVYSKKLVESGSFTEKETTQMEDEFKQYLNDRLEESKQQET
                     ASVTSFLEGVWSGVRRAEEKDFEKSPETGVSRKKFVEISELVTDLSRSGSPGADLKFF
                     AKIQKLYKNRRKMVTEKKQLDWGMAETMAFATLVTEGTPVRLSGQDSGRGTFAHRHAI
                     ITSEDNTKHVPLNYLFKDQAPFEVYNSFLSEYGVLGFEYGYAYAAPTSLTIWEAQFGD
                     FANGAQIIIDQFISSSETKWHRMNGVVLMLPHGHEGQGPEHSSARIERFLTLCAGNNM
                     QIVNCTTPANIFHILRRQMALPFRKPLVIFTPKSLLRHPLCLSLLDDFLEGTHFLEVI
                     DDDFVDASSVRKVLFCSGKVYYDLLECQQSSDVKDIAIVRLEQLYPFPKKQLLSIFKR
                     YSGAKQWCWVQEEPENMGAWSFVLRTFSETGKVLDLVARDADSSPAVGIPQVHTAEQK
                     NLVHRAFEE"
     gene            complement(3317..>4855)
                     /locus_tag="EYO68_08550"
     CDS             complement(3317..>4855)
                     /locus_tag="EYO68_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIC07891.1"
                     /translation="MKSFHVLPIYGGQEYSGQIRGLKRGSHVVVGTPGRVMDHMRRGT
                     LKLDKLSTLVLDEADEMLRMGFIDDVEWILEQTPKERQIALFSATMPQQIRRIATRYL
                     KDPVQIIIKDKTTTAPTIRQRFWPVSGMHKLDALTRILEAEPFEAILIFVRTKTATVE
                     LSSKLEARGYASSALNGDIKQSQRERTIENLKKGKLDILVATDVAARGLDVERISHVL
                     NYDIPHDTESYVHRIGRTGRAGREGDAILFVAPREKRMLNSIERATRQKIEMMELPST
                     EVINDKRIAKFKQDISDTLATEELGLFYQMIEQYQQEHNVPALEIAAALALLLQGNSP
                     FLLQKRAKQKTDPDWEKSDSNRVKERGRGKGTDKGTDKGKERSKGQGKERVRTHRKEL
                     PLEEGMVRYRLEVGHIHEVKPGNIVGAIANEAGLDSQYIGRINIYDDYSVIDLPDGMP
                     KDVLNDLKKVWVAGQQLRISNISKAPKSTKFSSRSKDRKKGKDTNKGTMRSKVSRKKP
                     VRRK"
BASE COUNT         1282 a         1196 c          912 g         1465 t
ORIGIN      
        1 ctattcccgc agtttcgcct tcctgcagca gaattttcag tctgcctgca ctttcggcaa
       61 tcaggggcaa agttgccttg tcagtttcaa tttcgcaaat cgtttcatca attagaacgt
      121 agtcgccgtc ggctttgagc caattcccga tttcaacttc agtaattgat tcgcctggac
      181 tcggaatttt aatttccaca atagacatag acaaaacctt taaatgggtt agttaataaa
      241 tatgatctcg catacagtct gaaatccacc ccgcacttaa atcatcaacc cggaaatgga
      301 aataagaaca atccaggggg taattttggt gcgggttcat tcctcaaaag ccctgtgcac
      361 cagatttttc tgttctgcag tgtgaacctg tggtattccg accgcaggag aactgtccgc
      421 gtcacgtgcc accagatcca gcactttccc ggtttcagag aatgtccgca aaacaaagct
      481 ccaggcaccc atattttccg gctcttcctg cacccagcac cattgcttgg ccccggaata
      541 tcgtttaaaa attgagagca actgcttttt tggaaatgga taaagctgct ctaaacgtac
      601 aatcgcaatg tctttgacat cactgctttg ctgacattca agtaagtcat aatatacttt
      661 accgctgcaa aacaaaacct ttcggacaga ggacgcatcc acaaaatcat cgtcgattac
      721 ttccagaaaa tgagttcctt cgagaaaatc atcaagcaaa ctcaaacaca atggatgacg
      781 taacaaactt ttaggtgtaa aaattaccag cggtttccga aacggcaatg ccatttgacg
      841 acgcaaaata tgaaagatat ttgcaggagt ggtgcagttg acaatctgca tattatttcc
      901 ggcacaaaga gtcagaaaac gctcaatcct ggctgaagaa tgttccggcc cctgcccctc
      961 atgtccgtgc ggcaacatga gtaccacacc attcatgcgg tgccattttg tttcagaaga
     1021 tgaaataaac tgatcaataa ttatttgcgc tccatttgca aaatcaccaa actgcgcctc
     1081 ccaaattgtt aaagaagttg gagcagcata ggcataaccg tattcaaaac ccagaacacc
     1141 atattctgaa agaaacgaat tgtagacttc aaagggagcc tgatccttga aaagataatt
     1201 caagggaacg tgttttgtgt tgtcctcaga ggtgataatt gcatgccgat gcgcaaatgt
     1261 tcctcttccg ctgtcctggc cgcttaaacg gacaggcgta ccctctgtta ccaaagtcgc
     1321 aaaggccatg gtttcagcca ttccccaatc aagctgtttt ttctctgtaa ccattttccg
     1381 gcggttttta tacagctttt ggatttttgc aaaaaatttc aaatcagcac cgggacttcc
     1441 ggatcttgac aagtctgtca ccagttcgct gatttctaca aatttctttc tggacactcc
     1501 tgtttccgga gatttttcaa aatccttttc ttccgctctc cggactccag accagacgcc
     1561 ttccagaaat gaagtaacag aggccgtttc ctgttgcttc gattcctcca aacggtcgtt
     1621 gaggtactgc ttgaattcat cttccatttg cgttgtttct ttttccgtaa aacttccgga
     1681 ttccaccagt ttcttactgt aaacttccct gacctttgga tgcctcgcaa tccgtcgata
     1741 caaatcgggc tgtgtaaatc ttggttcatc tgcttcattg tgaccatgcc gccggtaacc
     1801 caaaatatcg acaaatacat cccgatggaa ttcttgacga aactcaagtg cgagttttgt
     1861 aacataggct acagcctcaa tatcatctgc attaacgtga aatacaggtg aaagagttgt
     1921 ttttgccaca tccgtacaat aagtgctgga acgtccttcc ataaaatctg cagtgaatcc
     1981 aacctgattg ttaatcacca aatgtacagt tccgccagtt tcgtatcccg gaagtttcga
     2041 catttgcaaa acttcataaa tgataccctg gcctgccatt gagtgatctc cgtgaatgag
     2101 aatcggcacc agttttttaa cattgccctc gtgatattga tctattttgg cccgggaaat
     2161 tccttccaca accggattta ctgcttccag gtgtgatgga tttggggtca ggctcaggtg
     2221 cacttttttt ccactgcgca ctttcttgtc acttgaatag cccatgtggt atttcacatc
     2281 accggaaaat ccgtcgctac caaaggcctt tccttcaaat tcagcaaaaa tatcgctgta
     2341 ggtttttcct aagatatttg ccagcacatt taacctgccg cgatgtgcca ttccaatcac
     2401 aaactcttcc acaccaagtt cagcaccata ctcaagtatc atatcaagtg caggaataat
     2461 tgattctcct ccttcaagtg aaaatctttt ttgtcccaca aatttagtat gcagaaaact
     2521 ttcaaaggcc actgcttcat tcgttttacg taaaagctcg attttctctt cacgagaaaa
     2581 atttggtgtg ttgcggcagg attccatttt ttgctcaagc cagtttataa tctctattgt
     2641 ccttacaaat ttgtattcca ctccgatgga accacaatat gtttgttcca ataattcgta
     2701 aatttcacgc aaagttgcat ttccaattcc aacacgtgta cccgcatgaa actccgtatc
     2761 catatccgct tcacttaagc caaaactttc taggactatt tctccctcat gtatccgccg
     2821 aggacgtaca ggattggtgt ttgcgaacat gtgcccgcgc tggcgataag ctccaatcag
     2881 gttgctgact gccatctctt tttgaaactg ttctggaaca acttcttcag tatgatcaac
     2941 aaccggctta gcttgggctt cctgagaaaa atctgccttt gaaaattcaa atccttcaaa
     3001 aaaattcctc cactcctgat ctatagagtt ggggtcctgc tgatattgct gataaagccc
     3061 ttcaaatgcg ccaatatctg cgttatttaa atatgaaagt gggttcatga taattgtaaa
     3121 taaataagta atgctgtttt cataaaaagt ctcaaaaccg tgtcatttcg agccaagcaa
     3181 gaaatcctgt ataagcgacc acactgatat cataaatatt ttccaccgag gtcgaaatga
     3241 cagtgaggat ttcagactgt ttacgagaca gtcagtaata tcaagtaatt ttatgcttaa
     3301 tttcatgtca actaaatcat tttctacgta ccggtttttt acgacttact ttggaacgca
     3361 tagtcccttt atttgtatct tttccttttt tacggtcttt agagcgtgac gagaattttg
     3421 tggatttcgg agctttcgag atattggaaa ttcttaactg ttgtcctgcg acccaaactt
     3481 ttttcaagtc attcaacaca tcttttggca ttccatccgg cagatcaatt acgctgtaat
     3541 catcataaat attgatccgt ccgatgtact gactatcgag tcctgcctca tttgcaatgg
     3601 caccaacaat gttgccgggt ttcacctcat gaatgtgacc cacttcaaga cggtaacgta
     3661 ccatcccctc ttccagcggc agttcctttc tgtgagtacg aacgcgctct tttccctgtc
     3721 ctttgcttcg ttcttttcct ttatctgttc ctttatccgt tcctttacct ctacctcgtt
     3781 ctttaacacg attggaatcg gatttttccc agtcaggatc tgttttttgt tttgcccgct
     3841 tctgcaacag gaatggggag ttgccttgta aaaggagtgc aagagcagca gcaatttcca
     3901 gtgctggcac attgtgttcc tgttgatact gctcaatcat ttgataaaac aaacccagtt
     3961 cttctgttgc aagagtgtcg ctgatatcct gtttgaattt agcaatccgc ttgtcattga
     4021 tcacttcagt ggagggaagt tccatcattt caatcttttg cctggtcgca cgttcgatgg
     4081 aattcaacat ccttttttca cggggtgcaa caaacaaaat agcgtcacct tcacgacccg
     4141 cacgtcccgt tcgaccaata cgatgcacat aggattccgt atcatgcgga atatcataat
     4201 tcagcacgtg gctgatacgt tctacatcca gccctcgtgc agctacatct gtggccacca
     4261 gaatatccag ctttcccttc ttcaaatttt caatagtacg ttcacgttga ctttgcttga
     4321 tatctccatt gagcgctgag gaagcataac cgcgtgcttc cagctttgaa gacaactcca
     4381 ccgtagcggt ttttgttcgg acaaaaataa gtatcgcctc aaatggttct gcttccagga
     4441 tacgcgtcag cgcatccagt ttatgcatcc cgctcaccgg ccagaaacgt tgacggatcg
     4501 taggggcagt ggtggtttta tccttaatga taatttgtac agggtctttc aaatatcgcg
     4561 tggcaatccg tcttatctgc tgcggcatgg tagccgaaaa aagcgcaatt tgtctttcct
     4621 taggtgtttg ctcaagaatc cactcaacgt catcaataaa tcccatccgc agcatttcat
     4681 ctgcttcatc cagaacaagt gtgctgagct tatcaagttt caaagtaccg cggcgcatgt
     4741 gatccatcac gcgtcctgga gttccaacca ctacatgtga tccacgcttc aaacctcgta
     4801 tttgcccact atattcctga cctccgtaaa tcggcagcac atgaaaactt ttcat
//
LOCUS       DTVD01000237            4846 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6004_length_4846_cov_455.745147, whole genome
            shotgun sequence.
ACCESSION   DTVD01000237 DTVD01000000
VERSION     DTVD01000237.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6004_length_4846_cov_455.745147"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            224..1816
                     /gene="aceB"
                     /locus_tag="EYO68_08555"
     CDS             224..1816
                     /gene="aceB"
                     /locus_tag="EYO68_08555"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIC07892.1"
                     /translation="MNNIIITGKMHPGFDKILTEEAQEFLVKLHQRYNNTRKAVLDRR
                     NAIHHKILSGGKPIFLPETASVRKGNWKVDPIPDDLQDRRCEITGPAEAKMMINALNS
                     GAKIFMADLEDSITPSWFNQIQGQANISAAYERTLEFTSPERKEYRLNKGELATLIVR
                     PRGWHMEEKHILIDSEVASGSLVDAGLYLFHNIKRTLSYGTGPYFYLPKLENYMEARL
                     WDEVFAFSEQELGVTHGTIKATVLLETILAAFEIEEILYELREHMAGINAGRWDYMFS
                     AIKKFRHEPGFLWPDRAQVTMTAPMMRAYTELLVQTCHKRGAHAIGGMAAFIPSRRDA
                     EVNRVALAKVKEDKEREAQDGFDGSWVAHPDLVPVCTDVFSKVFEEGKVNQIHRLRED
                     VQVTPEMMLDFKIPGGKITEPGLRNNISVGIQYISAWLKGTGAVAIFNLMEDAATAEI
                     ARSQVWQWIQHSEGKLDDGRQITLEMVQTMIPEELAKIREAYGKAYDEEKMNQATDLF
                     TSLVSGDNFEEFLTIRAYDQLD"
     gene            complement(1804..2310)
                     /gene="folK"
                     /locus_tag="EYO68_08560"
     CDS             complement(1804..2310)
                     /gene="folK"
                     /locus_tag="EYO68_08560"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIC07893.1"
                     /translation="MLVHLGLGSNLGNRQDTLKQACVELESLPLLQFRVSPIYESEPL
                     LKMPQPKYLNMVVCGLTELSPLELLKKCQQIENRLGRVRNEHWGSRTIDIDILAYGEE
                     CIDAEILKIPHPEMEKRSFVLVPLLELSPEWVHPKTGISIRKLWEERLQTNDEELPQP
                     LNWKSQSN"
     gene            2416..2973
                     /gene="rsmD"
                     /locus_tag="EYO68_08565"
     CDS             2416..2973
                     /gene="rsmD"
                     /locus_tag="EYO68_08565"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIC07894.1"
                     /translation="MIRIIAGEFRGRKLKVPTGNDVRPTSDQVREALFNLLTSMLNWD
                     NMTVLDLYAGSGALGLEALSRGAKKAIFVESSKKHVSIILKNISMLSLEQTKYELVRG
                     RAESWIPNFADPERPCLIFLDPPFSSDEYDLILEKLAILPAILKGSLIVVESPVTREI
                     MFAENMELLKHRRYGSITLDILRKS"
     gene            3071..3883
                     /locus_tag="EYO68_08570"
     CDS             3071..3883
                     /locus_tag="EYO68_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIC07895.1"
                     /translation="MSYLDKIDKAALPKHVAIIMDGNGRWAIQRRMPRLQGHRNAVKA
                     VRACVEASAELGLQVLTLYAFSTENWQRPRKEVSALMDLFFEFLEKELHTMLDNNVRF
                     QLIGNREGLPDFLQNRLSHALDSTAANTGLLLNLALNYGGRDEMVRAVRRIAESVKKG
                     DLLPENIDESLVSANTYTAGQPDPDLIIRTSGELRLSNFLIWQAAYAEFYFTEVLWPD
                     FTKEDLYKALLEFQQRKRRMGRTTDSGSMTEAANVSHLVSKTEVTSILKTSG"
     gene            3892..4722
                     /locus_tag="EYO68_08575"
     CDS             3892..4722
                     /locus_tag="EYO68_08575"
                     /inference="COORDINATES: protein motif:HMM:PF01148.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIC07896.1"
                     /translation="MSDASSTSDLSARVKTGFPLAAGIAILLFTPALVIGLAVMAVAF
                     IGAQELTAMLSAKSDNSDTIVILPEWLLPGTAVLMGLGALAGESGLHGALLISALGWI
                     FFELVFTPKSELAKLSSLGFGLFGMVWAVWSVLHITLIKTLPEGTALLFFLLLVISFS
                     DIFAYFGGKRFGKSLLAPSISPKKTWEGSFFGVVGGGIVGAVFGELTMSMFWLYGLVL
                     AMLLAVVGQLGDLVESKIKRLCDVKDSGTLLPGHGGILDRIDGHMLAAPVFYYILQLG
                     "
     gene            4719..>4846
                     /locus_tag="EYO68_08580"
     CDS             4719..>4846
                     /locus_tag="EYO68_08580"
                     /inference="COORDINATES: protein motif:HMM:PF02670.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIC07897.1"
                     /translation="MKSSASTANGQEVPSSVVTKLTVLGATGSIGLNTLEIVRQFP"
BASE COUNT         1357 a          991 c         1157 g         1341 t
ORIGIN      
        1 ggaaaacacg caggtctaac atcgacggaa gagatcacac tttttgacgg aaccggagtc
       61 gtgtgtcaag atctcgcagt agcctctgac gcagtcgaac ttgctctcaa aacaggagat
      121 gcgatagaaa ttaaaagctt aagctctaaa gtattttatt gatcatctta gttatttcgt
      181 ttattcttat gcagttgaaa atgtaaaatt aattaggaga caagtgaaca acatcattat
      241 taccggaaag atgcatccgg ggtttgacaa aatactgacg gaggaagcgc aggaattttt
      301 ggtcaagctg catcaaagat ataacaacac ccgtaaagct gtgctcgatc gccgcaatgc
      361 aattcatcac aaaattcttt ccggcgggaa gccgattttt ttacctgaaa cagcatctgt
      421 ccgcaaagga aactggaaag tagatcccat tccagatgat cttcaggacc gccgctgtga
      481 aattacaggc ccagcagaag caaagatgat gattaacgct cttaattccg gagccaaaat
      541 attcatggcc gatttggaag actcgattac gcctagttgg ttcaatcaaa tccagggaca
      601 ggcaaacata tccgcagcct atgaacgtac acttgaattt acaagccctg aaagaaagga
      661 ataccggctc aataaaggag agttagctac attgattgta cgtccgcgag gatggcatat
      721 ggaagaaaag catattttga ttgacagtga agttgcttca ggcagtttgg tagatgccgg
      781 actgtatttg ttccataata tcaaacgcac attgagctat ggaacaggac cgtattttta
      841 tttgccaaaa ttggaaaact atatggaagc ccgattatgg gatgaggttt ttgctttttc
      901 ggagcaggaa ttaggcgtta cgcatggtac catcaaggct acggttctgc tggaaaccat
      961 tttagctgct tttgaaattg aagaaattct atacgaattg cgtgaacata tggccggaat
     1021 taatgccgga cgctgggatt atatgtttag tgcaattaaa aagtttcgac atgaaccagg
     1081 atttctctgg ccggaccggg cgcaagtcac catgactgct ccgatgatga gggcctatac
     1141 agaactgctg gttcagactt gtcataaacg tggtgctcat gccatcggtg gaatggcagc
     1201 cttcattccc agccgccgtg atgcagaggt aaaccgtgtt gccctagcca aagtaaaaga
     1261 ggacaaggag cgtgaggcac aggatggttt tgatggatca tgggtagctc atccggatct
     1321 tgtaccggtt tgtacggatg ttttcagcaa ggtatttgaa gaaggaaaag taaaccaaat
     1381 acaccgtctg cgtgaggacg ttcaggtaac acctgagatg atgcttgatt ttaagattcc
     1441 gggaggaaaa ataactgagc cggggttacg caacaatatc agcgttggta ttcagtacat
     1501 ttctgcatgg ttaaagggta caggtgcagt agcgattttc aatttgatgg aagatgcagc
     1561 gactgctgaa attgcaagat cgcaggtttg gcaatggatt caacattctg aaggaaaact
     1621 ggatgacggt cgtcaaatca cacttgagat ggttcaaact atgattccgg aggaacttgc
     1681 taaaatccgg gaagcttacg gaaaggccta cgacgaagaa aaaatgaatc aggcaacgga
     1741 tttatttact tcacttgtct caggagataa ttttgaggag tttctcacca taagggccta
     1801 tgatcagttg gactgacttt tccagtttag aggttgagga agttcttcgt catttgtctg
     1861 cagccgctct tcccaaagct tccggatact aataccggtt ttcggatgca cccattccgg
     1921 actaagttcc agtaggggca ccaaaacaaa gcttcgcttt tccatttcag gatgaggaat
     1981 tttcaaaatt tctgcgtcaa tacactcttc accataagct aaaatatcaa tatcaattgt
     2041 gcgggaaccc cagtgttcgt tacggacacg ccccagtctg ttttcaattt gctgacactt
     2101 tttcagcagt tcaagaggac taagttcggt caatccacaa accaccatat ttaaatattt
     2161 aggctgcggc atcttcaaaa gcggctcact ttcataaatc ggtgaaacgc gaaactgaag
     2221 cagtggtaga gactctaact caacgcaggc ctgtttcagg gtgtcctgcc tatttcccaa
     2281 gttgctcccc aatcctaaat gcactaaaat agcttcagtc atagtttgtt tttcaggtga
     2341 tcatgatttt tgaagaatta gcttaacaca agcacttatt cagtcgcaaa ctgaagcaaa
     2401 aactgttaca tagaaatgat tcgaattata gcgggagaat tccggggccg aaaactaaaa
     2461 gttccaacag gaaatgatgt ccggcccact tcagaccaag ttcgagaggc gctatttaat
     2521 ctgctgactt caatgctgaa ctgggacaac atgacagtgc ttgaccttta tgccggaagc
     2581 ggcgcgctgg gtttggaagc cttgagccgt ggtgcaaaaa aagcgatctt cgttgaatcc
     2641 tcaaaaaaac atgttagtat tattctgaaa aacatttcga tgctttctct ggagcaaaca
     2701 aagtatgagt tggtacgagg gcgggcggag agttggattc ccaattttgc ggatccagaa
     2761 cggccatgcc ttatttttct tgatccacct ttttcaagtg acgaatatga tctcatcctg
     2821 gaaaaattag caatcctccc tgctattctg aagggttcat taattgtagt tgagtcacca
     2881 gtcacaagag aaattatgtt tgcagaaaat atggaactgc tgaaacatcg ccgttatgga
     2941 tcaataacgc tggatatttt gcgtaaaagc tgagcctgta ttaacacaat aatcttgcct
     3001 tgacgtcggc actgtcctac ccttagaagg acaaataatt ggaaattatt atactttaat
     3061 ttttttcggc atgagttatt tagacaaaat tgataaagcg gctctaccaa aacatgttgc
     3121 cataatcatg gacggcaacg gacgctgggc catacagcgc cgcatgccga gactgcaggg
     3181 ccatcgtaat gctgttaaag cggtaagagc ctgcgtagaa gcttcagcag agttaggttt
     3241 gcaggtgttg actttatatg ccttctcaac agaaaattgg cagcgcccga ggaaagaagt
     3301 ttcggcactg atggatcttt tttttgaatt cttagaaaag gaactgcaca caatgctgga
     3361 taataatgta aggtttcagt tgattgggaa tagggaagga ttaccggatt ttctgcaaaa
     3421 tcgattgagc catgcgctgg attccacagc tgcaaatacc ggtttgttac tgaacctggc
     3481 acttaattac ggtggccgtg acgaaatggt acgtgcagtc aggaggattg cagagtctgt
     3541 taagaaaggc gatttgcttc cggaaaacat tgatgaatct ttagtatcag caaatactta
     3601 cacggcagga cagccggatc cggatttgat cattcgcaca agcggcgagc tgcgtctatc
     3661 caactttttg atttggcagg cagcatatgc agaattttat tttactgaag ttttgtggcc
     3721 tgattttact aaggaagatt tgtataaggc acttttagag tttcagcaaa gaaagcgacg
     3781 aatgggacgg acaacggatt cagggtccat gactgaagct gcaaatgttt cgcatttggt
     3841 ttccaagacc gaagttactt ccattctcaa aacatctggc tgaattttaa tatgagtgat
     3901 gcctcatcca cttctgactt gtccgcacgg gtcaagaccg gattcccact ggccgcagga
     3961 attgccatcc tgctctttac tccagcttta gtaatcggac tggctgttat ggctgttgca
     4021 ttcataggtg cgcaggaact caccgccatg ctgtctgcca aaagtgacaa ctcagataca
     4081 atagttatac ttccggaatg gttgctgcca ggcaccgccg tactgatggg gctgggagca
     4141 cttgccggag aatccggtct tcatggtgca ttgctgatca gcgctttggg ctggatattt
     4201 tttgaattgg tttttacacc aaaaagtgaa ctggctaaac tttcatcact tggtttcggt
     4261 ttgtttggaa tggtttgggc cgtttggagc gtcttgcaca ttacactgat caagacactt
     4321 cccgaaggta cggccctgct ttttttcctt ttactcgtaa ttagtttcag tgacattttt
     4381 gcctattttg gtggaaagcg ttttggtaaa tccctgcttg ctccaagcat cagtccgaag
     4441 aaaacctggg aaggcagctt tttcggtgtt gtgggtggag gaattgtcgg tgcagttttt
     4501 ggcgaactta caatgtcgat gttctggctt tatggattgg tgctggccat gctgctggca
     4561 gttgtaggtc aactgggtga tttggtagaa tccaaaataa aacggctctg cgatgtcaaa
     4621 gactccggga ctttgctgcc cggacatggc gggatccttg acaggattga cggtcacatg
     4681 cttgctgcgc ccgtatttta ctatattttg caactcggat gaaatcctca gcaagcacag
     4741 caaatggtca ggaggttcca tcctcagttg ttactaaatt aacagtcctg ggcgcaacag
     4801 gttcgattgg tctcaataca cttgaaatag ttcggcaatt tccgca
//
LOCUS       DTVD01000238            4843 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6009_length_4843_cov_259.322473, whole genome
            shotgun sequence.
ACCESSION   DTVD01000238 DTVD01000000
VERSION     DTVD01000238.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6009_length_4843_cov_259.322473"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="EYO68_08585"
     CDS             complement(<1..728)
                     /locus_tag="EYO68_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine monophosphate cyclohydrolase"
                     /protein_id="HIC07898.1"
                     /translation="MICTANNLNFTNTELTRETAGQNFKNHIAENVYPGRGIVIGRNH
                     ENSWIVIYWIMGRSSNSRNRFFRHENGILLTEAADPSLVEDPSLIIYNAMRDVDDCVV
                     VTNGSQTDTICEGFMQGESFYDSLYTEKHEPDAPNFTPRISGLIEQKKSSMTLAKISK
                     CDFSAEQSVYHFYRYNEIGAGFGYCLTTYMGDGNPLPSFKGDPILLPLEGDAEQIAEK
                     YWEALNQDNRISLAVRELTVSGKD"
     gene            complement(688..2157)
                     /locus_tag="EYO68_08590"
     CDS             complement(688..2157)
                     /locus_tag="EYO68_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIC07899.1"
                     /translation="MAKEIIINHTSHETRVAVIEGGELSELYHEREKTMRLVDNIYKG
                     KVLNVLPGMESAFVDIGSERAAFLHIDDILPEHEIFGEPSNNNGEQQKIGQLIQEGQD
                     VLVQVSKGPIGTKGARITSHVSIPGRNLVYLPATKTLGVSRQINQEKERERLKEIVDR
                     LRPENAGFIIRTVAEGHSEEDLHSDINYLVSIWDDILEKYKTLPAPSLLHSDLNVIFR
                     TIRDLFSADIRKLVVDDKSQFNKVRQFVRSYLPRYGSVIENYTKAEPVFDHYGIENEI
                     DRALGNKVWLRSGGHLVIDQTEALTAVDVNTGRFVGKESHEKTILRTNIEAAEEIVYQ
                     LQLRNIGGIIIIDFIDMETQQHRQMVYQTLKDQLRSDKARSKILQISEMGLVEMTRKR
                     DRENLNRYLCDPCPYCDGTGQIKSPSTVCYEAFREVRRSCRRNTKGSVAIFLHSDVFK
                     YIQEEEQEAVKLLEDEMGRSITIKLSDDLHREQFELYEY"
     gene            complement(2166..2888)
                     /locus_tag="EYO68_08595"
     CDS             complement(2166..2888)
                     /locus_tag="EYO68_08595"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIC07900.1"
                     /translation="MIALHINIDHVATVRQARQISEPDPVTAAGLVELAGADGITIHL
                     REDRRHIIDRDVRILRETIQTRMNLEMAATAEMFAIALDIRPDIVTLVPEKREEVTTE
                     GGLDIAGAAEIMHKGIAQLRGAGIRVSLFIDPDAEQIKASQKAGAEDVELHTGCYANA
                     ENSEELDREYQRLVSAAELAHQEKLQVNAGHGLNYINTQRICGLPHLRELNIGHSIVS
                     RAIFVGISQAVQEMREIIIRNS"
     gene            complement(3002..3907)
                     /locus_tag="EYO68_08600"
     CDS             complement(3002..3907)
                     /locus_tag="EYO68_08600"
                     /inference="COORDINATES: protein motif:HMM:PF07949.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HIC07901.1"
                     /translation="MRINVYFKVLAVVLAVFVWFLARKSGEPIQMSFYAPVVFKNVSP
                     SFQVTSDPPQVNIVVHTNSRDSFNPQELQAVLDLDKAQEGTLSYVLTENHILSPVKVQ
                     ITRIHPSQITVRIEELIEKTFSIKPRYQGTPKKGYLMRDIRIIPDTLTMRGPRSVLEK
                     LDHVSAHEIELEGLKESVTMRVDLDLPRGNVQIIDQNVDFYSAEITIDSLPIKKRFSK
                     VPVFLRNMDYVSVINPGTFNVFVEGPEDLIQELNRDELYGEIDLSTFEPGEYPKVTPK
                     VVKPDGITVLQQWPIVSVWVKNERN"
     gene            complement(3911..4816)
                     /locus_tag="EYO68_08605"
     CDS             complement(3911..4816)
                     /locus_tag="EYO68_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HIC07902.1"
                     /translation="METLLPIFTGNFTFRWQDLFDIVLLSIIFYRILLLIRGTRTIPM
                     LMGFVLLLALYLVSLILNLEATQLLLDNLGQSLVLVLVVLFQTDIRNGLAQIGVFTLF
                     RENSQQKRNLVESTLHACLQMSQQGIGALIVLEQEVGLKNFSDRGTQLNSTVSEQLLL
                     SIFHPSSPLHDGAVIISNKGDLLAAQCILPVSMNSRLSSSLGTRHRAAIGLTEETDAA
                     VLIVSEERREISLSYRGQLIRGANEDIRKALLDVLAGNIPDAIREKETKDTYRKTKLK
                     KAKKTTETSDDLKTPDNTPVETNKG"
BASE COUNT         1320 a         1123 c          968 g         1432 t
ORIGIN      
        1 ctgtcctttc cagagacagt caattcccga acggcaagag aaatacgatt gtcctgattc
       61 agcgcctccc agtatttttc agcaatttgt tctgcatcac cttccagcgg caaaaggatt
      121 gggtcaccct tgaaagaggg aagcggattc ccgtccccca tgtatgtggt caggcaatat
      181 ccaaatcccg ctccaatttc attatagcga taaaaatgat agacagactg ctctgccgaa
      241 aaatcacatt tactgatttt ggccaaagtc attgatgatt ttttttgctc aatcaatccg
      301 gaaatacggg gtgtaaaatt cggtgcatcc ggttcatgtt tttccgtgta cagtgaatcg
      361 taaaaacttt caccctgcat aaaaccttca caaattgtat cggtctgact tccgtttgtg
      421 acaactacac aatcatcgac gtcacgcatg gcgttgtaaa tgatcagtga cggatcttcc
      481 accagagaag gatctgctgc ttcggtaagc aatattccat tttcgtgcct gaaaaaacga
      541 ttcctgctgt ttgagctgcg ccccattatc cagtaaataa caatccacga attctcatga
      601 ttgcgcccta ttacgattcc gcgtcccgga tagacgtttt ctgcaatgtg attcttgaaa
      661 ttttgtccgg ctgtttctct ggtcaactca gtattcgtaa agttcaaatt gttcgcggtg
      721 caaatcatca gataatttaa tggtaatact ccggcccatc tcatcttcca ataatttgac
      781 ggcttcctgc tcttcctcct ggatatattt aaaaacatct gagtgcagaa atattgcaac
      841 agatcctttt gtgttcctgc ggcagctgcg gcggacttcg cggaaagctt cataacagac
      901 tgtggacgga gattttatct ggccagttcc atcacaatac ggacacggat cacaaagata
      961 acggttcagg ttttcgcgat cgcgcttgcg tgtcatttcg acgagaccca tctcacttat
     1021 ttgcagaatt ttgctgcgtg ccttatcaga acgaagttgg tccttcaggg tctgataaac
     1081 catttgccgg tgctgctgtg tttccatatc aatgaaatca ataataataa ttccgccaat
     1141 atttcgcagc tgcagctggt aaacgatttc ctcagcagct tcaatgtttg tacgcaaaat
     1201 tgttttttca tgactttcct taccgacgaa gcgtcctgta ttcacatcaa ctgcagttaa
     1261 tgcttcagtc tgatcgatga caaggtgacc tccggagcgc agccagactt tgtttccgag
     1321 agcgcggtca atttcatttt cgattccgta atgatcaaaa acaggttctg ctttagtata
     1381 attttcaatt acagatccat atcgcggcaa gtaagagcgt acaaactgac gaaccttatt
     1441 aaactgactt ttgtcgtcaa caaccaactt gcggatatct gctgaaaaaa gatcgcgaat
     1501 cgttcggaaa atcacattca gatctgaatg cagaagcgag ggagcaggaa gggttttata
     1561 tttttccaga atatcatccc aaatagaaac cagataattg atgtcagaat gcaaatcttc
     1621 ttcactgtgt ccttctgcaa ccgtccggat gatgaatccg gcattttccg gacgtaaacg
     1681 gtccacaatc tccttcaatc tctcacgttc tttttcctga tttatttgac gggatactcc
     1741 aagcgttttt gtagcaggca aataaacgag attcctccca ggaattgaaa catgactggt
     1801 tatgcgggca ccctttgtac caattgggcc ctttgaaacc tgcaccaaaa cgtcctgtcc
     1861 ttcttgaatc aactgaccaa tcttttgttg ttctccatta ttattagagg gttcaccaaa
     1921 aatttcatgt tccggaagaa tgtcatcaat atgtaaaaat gccgccctct cagagccaat
     1981 atcaacaaaa gcagactcca ttccaggcag aacattcaat acttttccct tgtaaatatt
     2041 gtccacaaga cgcatcgttt tttcgcgttc gtgataaagc tctgatagtt ctccaccttc
     2101 aatcacggca accctggttt cgtgagaggt gtgatttata ataatttctt ttgccattga
     2161 atagtttatg agtttcgaat tatgatttct ctcatttcct gcactgcttg agaaatacca
     2221 acaaaaatcg cccgactgac aatgctgtgt cctatgttca attcacgtaa atgcggcagg
     2281 ccgcaaatac gctgggtatt gatgtaatta agtccgtgtc cggcatttac ctgtagtttt
     2341 tcctgatgcg ccaattctgc tgcagaaacc agacgctgat attccctgtc cagttcttca
     2401 gaattttctg catttgcata acagccggtg tgcagttcaa catcctcagc accagctttt
     2461 tgacttgcct tgatttgttc tgcatccgga tcaatgaata aactcacccg gattcccgca
     2521 ccccggagtt gggctattcc tttatgcata atttctgctg caccggcaat atccagtccg
     2581 ccttccgtag tcacttcctc ccgtttttca ggaacaaggg tcacaatatc cggacgaata
     2641 tccagtgcaa tagcaaacat ttctgctgtt gcagccattt ccaggttcat tcgcgtttga
     2701 atggtttccc gtaaaatccg gacgtcccgg tcaataatgt gtcgacggtc ctcacgcaga
     2761 tgaattgtga taccatccgc tccagccagt tctactaatc ctgctgcggt aaccggatcc
     2821 ggttcgctga tttgacgggc ctgcctgaca gtcgccacgt gatcaatgtt aatgtgtaaa
     2881 gcaatcatta tttcggttta cagttctttt actgtattca aagtcctttc aaacaaattc
     2941 aaaattcaaa aactgcagcc gattttttgc tgttattcgt taaatgactg ctgccagtcg
     3001 tttagtttct ttcattttta acccaaactg aaactatggg ccactgttga aggacagtta
     3061 ttccatccgg ttttacaact tttggagtta ctttaggata ctctccgggt tcaaatgttg
     3121 aaagatcaat ttcaccataa agttcatccc gatttaattc ctgaatcaaa tcttcaggac
     3181 cttcaacgaa aacattgaaa gtaccaggat ttattacact gacataatcc atgttccgta
     3241 aaaaaaccgg aactttggaa aatcgtttct tgatgggcaa actgtcgata gttatttctg
     3301 cagaataaaa atcaacattc tgatcgatga tttgcacatt gcctcttggc aaatccaaat
     3361 caacccgcat tgtgacactt tctttaagtc cttccagctc aatttcatga gcagaaacat
     3421 ggtccagttt ttccagcact gagcgtggcc cgcgcattgt aagggtgtcc ggaatgattc
     3481 tgatatccct catcaggtac cccttctttg gagtaccctg ataacgtggt ttgatggaaa
     3541 atgttttttc gatcagttct tcaattctaa ccgtaatttg agacggatga atccgggtga
     3601 tttgaacttt taccggagat aaaatatgat tttcagtcag cacataagaa agcgttcctt
     3661 cctgcgcttt gtcgagatca agaaccgcct ggagctcctg aggattaaat gaatccctgc
     3721 tgttggtgtg taccactatg ttgacctgcg gcggatctga agtgacttga aaggacggag
     3781 aaacattttt gaaaacaaca ggagcataaa aactcatttg aataggttct ccggatttcc
     3841 gggcaagaaa ccagacaaag acagctaaaa caactgcaag cactttgaag taaacattta
     3901 ttcgcatctg tcagcctttg tttgtttcta caggagtgtt atccggagtt tttaaatcat
     3961 cggacgtttc cgtggttttt tttgctttct tcaacttagt ttttctataa gtgtcttttg
     4021 tttccttttc cctgatggca tcaggaatgt tgcctgcaag cacatccagg agggctttgc
     4081 gaatatcttc atttgcaccg cgtatcaact gtccgcggta actgagactg atttccctgc
     4141 gttcttcaga aacaatcagg actgctgcat ccgtttcttc tgtcagacca attgcagcac
     4201 gatgcctggt tccgagagaa gagctgagac ggctgttcat cgaaacaggt aaaatgcact
     4261 gtgcggccaa caaatcaccc ttgttgctga taataacggc gccgtcgtgt aaaggcgatg
     4321 atggatgaaa aatactcagc aaaagctgtt cactgacagt tgagtttaat tgtgtacccc
     4381 gatcactgaa gtttttcaat ccaacttcct gttcaagaac aatcagggct cctataccct
     4441 gctgtgacat ctgcagacaa gcatgcaggg tgctctctac caaatttctt ttttgctgac
     4501 tgttttcacg gaaaagagtg aaaaccccaa tttgtgccag tccgttacga atatccgtct
     4561 gaaacagtac aaccagtacc agcaccagtg actgccccaa attatcaagg aggagctgtg
     4621 tggcttccaa attcaaaatc agcgaaacaa gataaagtgc cagcagcaaa acaaaaccca
     4681 ttaacatggg aatggtacgt gtgcctctga tgaggagcag aatgcgataa aaaattatcg
     4741 ataggagaac aatatcaaac agatcctgcc agcgaaaggt aaagttacct gtaaaaattg
     4801 gcaaaagtgt ttccactgaa agtctccgaa aagggaaagg ggg
//
LOCUS       DTVD01000239            4825 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6050_length_4825_cov_451.880084, whole genome
            shotgun sequence.
ACCESSION   DTVD01000239 DTVD01000000
VERSION     DTVD01000239.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6050_length_4825_cov_451.880084"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            163..1512
                     /locus_tag="EYO68_08610"
     CDS             163..1512
                     /locus_tag="EYO68_08610"
                     /EC_number="3.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIC07903.1"
                     /translation="MKMEYNDMQEGVKGIGTANQDFKVADINLAELGRKSITMAEHEM
                     PGLMMLREEYKGKRPLSGARITGSLHMTVETAVLIETLVDLGADVRWSSCNIFSTQDE
                     AAAAMAEAGIPVFAWKGETEEEYWWCLEQTLEWPEGQGPNLLLDDGGDLTGYVHEKRP
                     DLLKEVNGVSEETTTGVHRLKQMDRQGLLKIPAFNVNDSVTKSKFDNLYGCRESLIDG
                     IKRATDVMIAGKITVVAGYGDVGKGSAQSLRGQGARVLVTEIDPICALQASMEGYQVV
                     TMEDAAPVGDIFVTTTGCADIICREHMDAMKDQAVVCNIGHFDIEIDVASLNDDSSLK
                     KINVKPQVDQYVWPDGKRMILLAEGRLVNLGCATGHPAFVMSNSFTNQVMAQIDLWEN
                     GSKYENKVYVLPKLLDEKVARFHLDKLGVKLTRLSEKQAKYLDIPIEGPYKPDQYRY"
     gene            1525..2431
                     /locus_tag="EYO68_08615"
                     /pseudo
     CDS             1525..2431
                     /locus_tag="EYO68_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308066.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2434..3027
                     /locus_tag="EYO68_08620"
     CDS             2434..3027
                     /locus_tag="EYO68_08620"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIC07904.1"
                     /translation="MTGALLLNKTEIRKTLLKQRRGLSASKKKQYELQMLNSLRTWDV
                     FKEACIIHIFLSKADEPETSQIIELAWKSGKQIGVPCVLPDTLELFHSQLNSFEDLRP
                     GALGVLEPSPEQRTALTPESFDLVIVPGVAFDRQGGRLGYGKGYYDRFLDQSLAFRLA
                     LAFDFQILETVPTEMHDVPMDGILTENGFYFCGDSQT"
     gene            3024..3668
                     /locus_tag="EYO68_08625"
     CDS             3024..3668
                     /locus_tag="EYO68_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIC07905.1"
                     /translation="MKKWLIVVIIAIGLLATAISQYKSILTSYAEFFTVNNAISGADA
                     IVVLSGGKATRIPHALKLFAQGYAPRLLLTDEKKRNIRFAHLFSTNEEIAQAMIEELK
                     MNVQIKTVPSLKKGATSTFDEAYDLLQLSKIEGFNHLILVTDAFHTRRAYHAFQTVFE
                     GTEIRLEMSAAQNEIFTESNWWTSDQGISAYVLESIKYPVYLLSSRNATFIRND"
     gene            3668..4105
                     /locus_tag="EYO68_08630"
     CDS             3668..4105
                     /locus_tag="EYO68_08630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HIC07906.1"
                     /translation="MTESSLLIRPFQTEDEDALVALWKMCELTVPWNNPHKDIARKLQ
                     VQPELFLVGILDSSLIATVMGGYEGHRGWINYLAVHPDFQGKGYGQEIMNSVETGLRE
                     MGCPKINLQIRTGNDKIASFYQKLGFTNDHVVSMGKRLEADHS"
     gene            4151..>4825
                     /locus_tag="EYO68_08635"
     CDS             4151..>4825
                     /locus_tag="EYO68_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIC07907.1"
                     /translation="MPELLMIALYITGGLVLLYFGADWLVQGAVTLALHLGLSPLIVG
                     LTVVALGTSVPEALVSVQAAIGHQGGIALGNVIGSNILNIALILGLSSLILPLKVDSH
                     IVKADVPLLTGATFMLVVLLEDFHISRMEGAFLLLCIVFYVAGNIMTVKRTSPEEDKI
                     EGMEIPEDSGKTLWRDVGFLILGIVTLGFGANFLVTGAVDLARIFGLSEALIGLTIVS
                     IGTGTPE"
BASE COUNT         1411 a          925 c         1128 g         1361 t
ORIGIN      
        1 taattcagat ttgctttaaa cattaaatca ccacatgttc atataatgat ataacttgca
       61 ttcctgtgca gaattaaata taattcagaa taatcttaaa aagtgctttc tttgatttga
      121 gattaaatat tattaatttt aaacaattat tcaggagata aaatgaaaat ggagtataac
      181 gacatgcaag aaggagtcaa agggattgga actgcaaatc aggatttcaa ggttgcagac
      241 attaatttgg cggaactcgg acggaagagt atcacaatgg cggaacatga gatgcctggc
      301 ctgatgatgc ttcgggaaga gtacaaaggt aagaggcctt tgtcaggtgc ccgcattact
      361 ggatcattgc acatgaccgt ggaaacagca gttttgattg aaaccctggt tgacttgggt
      421 gctgatgttc gatggtcatc ctgcaatatt ttttcaactc aggatgaagc agcagccgca
      481 atggcagagg ctggcattcc agtatttgcc tggaaaggtg aaaccgaaga agaatattgg
      541 tggtgtctcg aacaaacttt ggaatggcca gaaggacaag gcccaaactt actcctggat
      601 gacggaggtg atcttaccgg ctatgtacat gaaaaacgtc ctgatttact gaaagaagtc
      661 aatggagtct ctgaggaaac tacaacgggt gttcatcggt taaagcaaat ggacaggcag
      721 ggcttattga aaattccggc attcaatgtg aacgactcgg ttaccaagag taaatttgac
      781 aatttatacg gttgccgtga atcactgatt gacggaatca agcgcgctac tgatgtgatg
      841 attgcgggaa aaattacagt agttgccggc tatggtgatg taggcaaagg ttctgctcaa
      901 tcgttacgag gacaaggtgc acgtgtccta gtaacagaaa ttgacccgat ttgtgcatta
      961 caagcttcaa tggaaggata tcaggttgtc accatggaag atgccgcacc tgttggagac
     1021 atatttgtta ccacaacagg ctgtgctgac atcatttgcc gtgagcacat ggatgccatg
     1081 aaagatcagg cagttgtctg taatatagga cattttgaca tcgaaatcga tgttgcctca
     1141 ctgaacgatg attcttcact taaaaagatc aacgtcaagc ctcaggtgga tcaatatgta
     1201 tggccggacg ggaagcgtat gattttactg gcagaaggac gtctggtaaa tttaggctgt
     1261 gcaacaggtc atcctgcttt cgttatgtca aatagtttca caaaccaggt aatggctcaa
     1321 attgaccttt gggaaaatgg cagcaagtac gagaataagg tatatgtact gccaaaatta
     1381 cttgatgaaa aagtcgcccg attccattta gacaaattag gagtgaaatt aacccgtctg
     1441 agtgagaaac aagcaaaata tttggatatc ccaattgaag gtccttataa gccggatcaa
     1501 tatcgttatt aagaggttat tttgatgtta agactgttca acattttcag tcgaaaatat
     1561 ttgataattg ggcttggatt tgcacttttg ctgttcaccg gtggatgtgg ttccaagcca
     1621 atcatgctca tgaacgcccc tttagataac ggtgaggatg ttcccattcc tccaactccg
     1681 gaagaactcc tgacatcaaa atcatttatc gcttttgttc cggcacaatt ttcagagaat
     1741 ctttcccgct accacaaggc actgagtgtg agctttgtgc agtctgtact ggaggagcat
     1801 ggagatctca aaataattga cgaagtccgt gttaagagtg cattgaatcg gagtgagttt
     1861 gcaaaactaa gtgcttcttt aaagcaaagt aaattacgca gttttgaaga cgacctggta
     1921 aaacaaacca ttgaactggg caagtggctg cgggtacgtt atatagtttt aatgagggta
     1981 caaccaagtc cagaaaaggt ttcgtcaaat gattggagca cctatatcag attcaggatt
     2041 taccgggttg aagatcctgt aaggagcgaa atgaatcacg aattcacttt tgttttttcg
     2101 gagtcaaata atctttggga agaacttggg ggggcttgtt cgtggtcgtt tccctttagg
     2161 tggatttatc atcgagtcac gtgcaaatag agcttatgta cgcattaatt tgggaaggct
     2221 caacagagtc tcggtcgaac aagagtgcaa aatattccgc agggttctca agaaaaaaaa
     2281 gggatcaaac ggaaatatta tttccagcat tgagtttgat agaatagggc aattgacttt
     2341 atttcgcgtg caggaggatt tttcctgggg aaaagttcct tcaaattttc gaggcaccat
     2401 cctgaaaggt gatgcagttc gctgttactg agcatgacag gggcgttgct tttgaacaaa
     2461 actgaaatac gcaaaacact gctgaaacaa cgcaggggat tgtcagcatc aaaaaaaaaa
     2521 cagtatgagc tccagatgct gaattccctg cggacatggg atgttttcaa ggaagcatgc
     2581 ataatccaca tttttctctc taaagcagat gaaccggaaa ccagccaaat tattgaactg
     2641 gcttggaagt ccggaaaaca aatcggtgta ccatgtgttc taccggatac gctcgaactc
     2701 ttccattctc aactgaattc tttcgaggat ttacgccccg gtgctttagg cgttttagaa
     2761 ccttcgccag aacaaagaac agctttgact ccggaaagct tcgatctagt aatagttccg
     2821 ggagttgcct ttgaccgtca gggcggaagg cttggctacg gcaaaggcta ctatgatcgt
     2881 tttctggatc aaagtctcgc ttttcgttta gcattggcct ttgattttca aatacttgaa
     2941 acagtaccca ctgaaatgca tgatgtacct atggatggta ttttgaccga aaatggattt
     3001 tatttctgtg gagattcaca aacatgaaaa aatggctgat cgtcgttatt attgctatag
     3061 gactgcttgc aaccgcaatt tcacaataca agtcaatcct gacatcatac gctgagtttt
     3121 ttactgtaaa taacgcaatt tccggtgccg acgcaattgt cgtgctttcc ggtgggaaag
     3181 ccacccgaat acctcatgcc ttaaaactct ttgctcaagg ctatgcccca cgcctgttat
     3241 tgaccgatga aaagaaaaga aatattcgtt ttgctcatct gttttcaaca aatgaagaaa
     3301 ttgcgcaagc catgattgaa gaattaaaga tgaacgttca aattaaaact gttccttcac
     3361 taaagaaagg tgctacttca acatttgatg aagcatacga tctactacaa ttaagcaaaa
     3421 ttgagggatt taaccacctg attttagtga ctgatgcttt tcacacacgt cgtgcatacc
     3481 atgcatttca aactgttttc gaaggaacag agatacgcct ggaaatgtct gcggcacaga
     3541 atgaaatttt tactgaaagt aactggtgga cttcagacca gggaatttca gcctatgttt
     3601 tagaaagcat caagtatccc gtttatctgc tatcctccag aaacgcaacc tttatccgca
     3661 acgactagtg accgaaagtt ctctcttaat ccgcccattt caaacagaag atgaggatgc
     3721 tttagttgca ctatggaaaa tgtgcgaatt aacagttcca tggaataatc ctcataaaga
     3781 cattgccaga aagcttcagg ttcagcctga actatttttg gtcggtattt tggatagtag
     3841 tttaattgcc accgtgatgg gaggttatga gggccaccgc gggtggataa attatttggc
     3901 ggtccatccg gattttcagg gaaaggggta cggacaggaa atcatgaatt ctgtggaaac
     3961 cggactgcgg gaaatgggtt gtcccaaaat caatttgcag attcgtacgg gaaacgataa
     4021 gatagcatcc ttctaccaaa aacttggttt taccaacgat catgtagtca gtatgggaaa
     4081 acggctggaa gccgatcatt cctgaaatac cctataaatt acaatcgtga ccacttgctt
     4141 gagataagtt atgcctgaat tactaatgat tgcactctat atcacaggtg gactggttct
     4201 gctttatttt ggtgcagatt ggttagtaca gggagcagtt acattggcct tgcatttagg
     4261 gttaagcccg ttaatcgtcg gcctgaccgt cgttgcattg ggaacgagtg ttccggaagc
     4321 actagtcagt gtacaggctg caataggtca tcaaggagga attgcacttg gaaacgttat
     4381 tggttcgaat atcctgaaca ttgcgttgat tctcggactc tcctccctga tccttccttt
     4441 gaaagtcgat tcgcacattg taaaagccga tgtgcctttg ctgactggag ctacttttat
     4501 gctagttgtc ctgcttgagg attttcacat ttcccgcatg gaaggcgcgt ttttgctctt
     4561 gtgcattgtg ttttatgttg ccggaaacat catgacagtc aaaaggactt ctccggaaga
     4621 ggataaaatc gaggggatgg aaataccgga agactccggc aaaactttgt ggcgggacgt
     4681 cggtttttta atattgggga ttgttacatt aggttttggt gctaattttc tagtaaccgg
     4741 tgcggtagac cttgcgagaa ttttcggact gagcgaagcc ctgattggac tgaccattgt
     4801 ctccatcggc actggaactc cggaa
//
LOCUS       DTVD01000240           47207 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_60_length_47207_cov_394.117408, whole genome shotgun
            sequence.
ACCESSION   DTVD01000240 DTVD01000000
VERSION     DTVD01000240.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 47207)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47207)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47207
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_47207_cov_394.117408"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="EYO68_08640"
     CDS             complement(<1..748)
                     /locus_tag="EYO68_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07908.1"
                     /translation="MLNHREYFGICLLICCVVFATAVWSEQPKNTVKNINPSPEILGA
                     MIPEIQQQFMNGLAEYAAKYQASPNAVQKFLLRQKRQQFLAEQLKDRVLTKWIGRIRT
                     LLTTGNGKAYLEIELAMVPPENATENKTAPEFRVTMGTWNNTHTDLDYDTLILPGTSL
                     HSWLANFNEGQWVFFSGNSFAGDEDYLKEASPTETEAMLSPQFILKFELLDSVDFPES
                     DLQLIESLPKPETPVTDAIAKSQDLPVSKSK"
     gene            complement(846..2483)
                     /locus_tag="EYO68_08645"
     CDS             complement(846..2483)
                     /locus_tag="EYO68_08645"
                     /inference="COORDINATES: protein motif:HMM:PF06739.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07909.1"
                     /translation="MSWFSQMKNKINKLNLTTKITCSVVFVSFLMNVLLGGCGGTANV
                     VVKKPKNTSLITPKLVSIEPRDNAITVKRNEKIVLKFSVPIDPLTLTVNTEDTQCSGT
                     IQISSYNFRNCVRMNLPELEDGNKRIVLSPKGVYAAQEFHQIRLSTEIKAINGASLKK
                     NLTTKLGFRTTWSQQIGTQGDDTGFAITVDKDGNVYLAGNTSVSESGEESDLFLAKYA
                     PSGFLNWIHQPGFGRSVKAAVLRMKKSGQLSLSGLSQGKNNSAVIKLTYDVNGKKLSS
                     KVIGISGTVSGNGMALDSEGHIYVSAASPFNLMKIRKTGEKMWATELNPGISLQAIAA
                     DSGTGLYLTGSMMRSLDGNKSTGGIDVFLLKMYKMGPKRWSKTFGTKLDDSGTSLAIL
                     SDEAVALVGYTTQTESNGTDMQKNYDAFVAKYNSEGTKLWTYIFKGTKSEQCTVTLWT
                     PEGNLLVGGYTESSMEKQSHFGKEDAFLAMLDSGGELLWLRQFGTPENERPLGLAIGT
                     EGQIYVTGFTEGRMDGAKYSGGKDIFLVQFNKKGEKQ"
     gene            2585..4600
                     /locus_tag="EYO68_08650"
     CDS             2585..4600
                     /locus_tag="EYO68_08650"
                     /inference="COORDINATES: protein motif:HMM:PF13387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HIC07910.1"
                     /translation="MLEINATQNDTLSKIRVFISHTFRWRRWLLLWLMFSASSIQAQL
                     ITQSSGHELADEKTVFLSLWKQADERQLTLHPYWLKLLHFYSFGESVGQWSFKSDIVS
                     SGFFLSQEGKIDPTAELKATLKALLSPLSGDPNLHARCKFIARYNWLRSNLDFPELPK
                     LSCPLFERWSNLEEATGISIVFVSSYLKNPASTFGHLLLKFNSQNRYFGHSLLRPTLN
                     FGAMINPDDNPLIYALRGLFGGYDGRFTDERFYNFNHVYGENELRDLWEYPLYFTVEQ
                     QHRVAYHAWELLQNVNFTYYFFLDNCAYRMAELLEMAWTDTTRINTSGAIWAIPVDVV
                     FKLKNIKKESGEQSLLGDPKLIPSRQRKLQNRTAQLSEMEQKQLTKLIESENYLDSEE
                     FRSFPEQSRARIIDTLLDYRQYVKRDKPTLNQQKERSVLLRVRSELPILENNVSAEIP
                     EAPTDGTPPMRFRFGAVSNSLLGPALEVGTWANYHDLLGDESGHLQNAEVVTLDLHLQ
                     LRKNSFEVTQFQLFNIQKFALNPTGISGDYEWSWRARGGWEREHLGCLPCRKFSIAGG
                     FGRSVSIGGKDLEYLFLDLFGETKNHSWPATTWGYAPHLGMLWSVLDIWKIRFDGGWF
                     KSIYGPKIEYFHIRFDQRLTIAQDWDIRMEIEQLGSLEGTLALHYFW"
     gene            complement(4734..5363)
                     /gene="lptC"
                     /locus_tag="EYO68_08655"
     CDS             complement(4734..5363)
                     /gene="lptC"
                     /locus_tag="EYO68_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIC07911.1"
                     /translation="MESQGLQTYSSFLHENRKELTILLLLGALAGAAYYLFSFSEPPE
                     NIPFEERGVLDLEQITVNDYQLERRSWKLKGKRAVISEKSSRMRIEQVTIWVFVSDNS
                     SVKPSPNLPLSTNLPTQEVDLFITAEQGLIEWDDNRVTLTGNVVLLRNDGSEVHTETA
                     IYNAKQEILTIPKPVRVLREGHTLHGSGLIYNVSKGELNLKQPVLLRHE"
     gene            complement(5385..5771)
                     /locus_tag="EYO68_08660"
     CDS             complement(5385..5771)
                     /locus_tag="EYO68_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07912.1"
                     /translation="MISAQEEVIPLGGPKKPVMYSVFWNTLWGSAWGATMGFSYHLVS
                     GIQLRESLITSTTLGGVLGYGLGIYLVVGGLSFDKSYLLELPSPQSSTQQPNTAFIES
                     EMMPLYSRAVKPAPLKWEVPIFAFRF"
     gene            complement(5866..6303)
                     /locus_tag="EYO68_08665"
     CDS             complement(5866..6303)
                     /locus_tag="EYO68_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07913.1"
                     /translation="MKKLRLWKIKWILPFFFLLMITASLQAQETGPLMMKQELGYTLG
                     GGVFGAGLGVVVWFMDPLNPSVTLKSTVKNGFIAGTALGALFGFYMLQNAIIVPSEST
                     LPENLDQLLGKDDLLRPIQSSIISQNEIKSVQNFRLPIFGFRF"
     gene            6664..7203
                     /locus_tag="EYO68_08670"
     CDS             6664..7203
                     /locus_tag="EYO68_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07914.1"
                     /translation="MLDGGDEFGFGQFGDNDFQSGDFGSGGFQDFGTQQTQDPQDPFD
                     SQFDSTDQYLDEGAFSDESESTLKDDLIVRRELLSQEGKAVPSNLGYGAGTGLMIGTW
                     FAFIRKETNTRQQFRTIGTSTVVGALIGIMLGTRSVWDPGAPRPEDDASPTGFSQSPA
                     GWLFAQNKESLKIAYRWKF"
     gene            complement(7292..8224)
                     /locus_tag="EYO68_08675"
     CDS             complement(7292..8224)
                     /locus_tag="EYO68_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC07915.1"
                     /translation="MDINNKAKIVILIPCLNEEITIGKVIHDFQDAIPEAKIIVFDNN
                     STDRTAAIARECGAEVIPESRPGKGHVVASMFRKVDADYYVIVDGDDTYSAEHVRKLL
                     EPVMKEQADMAVAVRLAEYTATSFRPLHVFGNNLVRRLVNWIFKSNLSDIMSGYRAYS
                     RELVQSIPILSSGFEVETEMTIRILDYGFRIKEVPLPYRERPEGSVSKLRTFHDGFRV
                     LAEIARIAKAYKPFTFFGGIGLAFILAGGISGIWVILDYLEDEYVNKVPTAILATGFM
                     LLGFGSMGIGILLNTISYRFRENMRQLHKIIRSR"
     gene            complement(8249..8947)
                     /locus_tag="EYO68_08680"
     CDS             complement(8249..8947)
                     /locus_tag="EYO68_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIC07916.1"
                     /translation="MFEVPPDADISLIRRAFRRKAKSCHPDLFQQVSEEERKRQQKIF
                     VRLSQAYEVLADPEKRNILDRQLGKSGAKASQPHDQKNRTSSSSSSSRRGYKQKPEFR
                     GNSHESATLEQDDTLEDLLRDAGEMLGQFGLSFRDPLEIFVDWARQIFQEVTESFEDN
                     ADYRTSTESSNHKTQTKSYTDKDPLDNIEAELKRLKKQQVKATSKSAFSVRSNIKDTD
                     IEQELRSIKKKYKL"
     gene            8996..9601
                     /locus_tag="EYO68_08685"
     CDS             8996..9601
                     /locus_tag="EYO68_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIC07917.1"
                     /translation="MNRILYFDLETKYSADEVGGWGNIEDMGMSIGVIWDTLDEKFHV
                     YIEHQIKEMVDHFYRADQIVGFNHVGFDYRVVAGVRYADSHERSRLHTELAGLKNFDM
                     LLELKKLLGHRLKLESIARPTLGTGKSADGLQALKWYKEGKLDKIIAYCKVDVEVTRD
                     VHLYALENGKLHYDSRSGIKTVSVDWNSQPKKQEPQQMSLF"
     gene            9689..10489
                     /gene="pyrF"
                     /locus_tag="EYO68_08690"
     CDS             9689..10489
                     /gene="pyrF"
                     /locus_tag="EYO68_08690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIC07918.1"
                     /translation="MTFFKKLEKRVSARQTYLCVGLDPVWGKIPEHLPHSQEGLLEFL
                     LQIVNETKDHVAVFKPNFAYFEALGPSGMEVLQELIHKIPPEIPVIGDAKRGDVGHSS
                     RMYAKAVFETFGCDAVTVTPYLGHDALEPFLDNPDRGVFVLCLTSNSGANDFQLPDLH
                     LKVAKKVREWNIYGNAGLVVGATRPENIAAIREISGRMPYLIPGIGTQGGDLRNTLFY
                     ADDGSKIPYLISTSRSILYASSKEDFAQQAGTTARKLQDQINLCRSSL"
     gene            complement(10464..11654)
                     /locus_tag="EYO68_08695"
     CDS             complement(10464..11654)
                     /locus_tag="EYO68_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIC07919.1"
                     /translation="MRNLTILKRYSIIHGALLIFALATTNQAFSAESVNRYRSHYKAA
                     ILADAESGRILLHDRMHQKNYPASLVKMMVALIALEEIESGRISLQDKVNISRWTSKI
                     GGHQVYLKQGEVFKFRELMKATVISSANDAAVAVAEHVSGQVKFFLKKMNERADELGM
                     KKTRFYSVHGLPPGRGQKIDVSSAYDLYLLALELLKHPLYLSWSSIRLDTFRNGTFQL
                     LNTNHRMIRNYRGMDGMKTGYHRRAGFNLVSTAKRKGQRYITVVLGAKNSRLRSKATK
                     NLLDYGFENYLKYELNKKGDTVPFSVSVENGEAEGVSLQTTDAVTILLSQEEHKRLEI
                     WPQIPKQITAPVKVEQQLGSLEYRLDGKLLGQVKLQTENAVPVQSFLSGGFTSMLTNL
                     SDTN"
     regulatory      11903..12006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12128..13141
                     /locus_tag="EYO68_08700"
     CDS             12128..13141
                     /locus_tag="EYO68_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC07920.1"
                     /translation="MKSIKLSVLPMLLIVVGIFINPTWGAELTIYTYDSFNSEWGPGP
                     VVFKRFEKQCACKLKVVSPGDSGTVLNRVILEKANPKADILLGLNNSELEKSFSYDLW
                     EPYRSPLLEQVPADLRVDKKHRVTPFDYGFIAFVYNSQKIKNPPNSLQDLLDPKYRRK
                     IVIENPKTSSPGLSMLHWTIAVFGEEDYLDYWKKLEPNLLSVTNGWSAAYGMFTKGEV
                     PIVLSYVTSPAYHLEYEKTERYRTAVFQDGHYRQIEFAGILKGTKRIKRAREFIDFML
                     SEKFQNAIPLTNWMFPVIQHQPLPDSFRIAPQPKRAPELNPARVNQQNSKWLKEWSRT
                     MSQ"
     gene            13200..14822
                     /locus_tag="EYO68_08705"
     CDS             13200..14822
                     /locus_tag="EYO68_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HIC07921.1"
                     /translation="MLGAGFYWPLLSFLSGNNPLHPEESFLQWKFFKEFLSDPWNLRV
                     ISFSLYQAFLSALLSILVGLPGAWLLTYYNFPGKRWFRLLTYLPFILPSILVVLAMVL
                     FYGNNGWINRGLMTILGTDEPQVHFLYSLTGILIAHVFYNFPLAMKIIGDQWERISLK
                     YHHAASSLGVGNMRRFFGITLPLLMPSIGSAFVLIFLLCMNSFAIILVLGGGIRYTTI
                     EVLIYQLARIELDFSGAASLAFLQGGLSLIGMAIMLKGRDRRVDQESASKSWLPEKLR
                     NRSMKAWFGLCWILLVLIFALGPLFAILMDSFRKFEHGEWIYTWYWYSELFTWRENNY
                     FLSSLWNSLRIGLGSALISSISGLGLVSIIAYRKGVKRRFWEMLTLFPIALSTVVFGV
                     AWFHFYQGHLAGTVPLIFVVMAMHALLTCPYWIRIVLPTLESIPLQWHTESKMLGKQP
                     LEYGVRILWPWLSKTFLIAFFFSFSLSLGELNSTMMIADETVRTLPLEIYGAISGYRF
                     SYASAVAVILLILSVSTFLLVERSIGFFGRLK"
     gene            14934..15341
                     /locus_tag="EYO68_08710"
     CDS             14934..15341
                     /locus_tag="EYO68_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07922.1"
                     /translation="MEVGGIREETCTAEETACEKINASVVEQELETAILEYAISTFPF
                     SSAFENVPGYREEELPDLIEGLISEGIVKVGFYYEEMQENLTILYFLRLDEEKHVVLG
                     LDPNEVCVSISNVDGIFRLNDDQYMNLLKIPLC"
     gene            15391..16077
                     /locus_tag="EYO68_08715"
     CDS             15391..16077
                     /locus_tag="EYO68_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07923.1"
                     /translation="MSELSDPPTLLKTEAIQLSLIGMSGAGKSHWSKRMESLGFQMYS
                     CDDLIAERLGQKLEEKGKSTINLAKWMGQPFSEGYPEAEAFYLELEGAVISQICDELE
                     NSSQKDKQVVVDTTGSLIYLEKKLLNRLRNLTLTVQLKLPEEKHEQLFEAYLLDPKPV
                     IWGGEYLPREGESPQNALGRCYRELLSFRNERYGLLADCVLDYSFHHCAKTGVEELLE
                     LVTNNYKMKP"
     gene            16337..17029
                     /locus_tag="EYO68_08720"
     CDS             16337..17029
                     /locus_tag="EYO68_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HIC07924.1"
                     /translation="MEIFTLDGWYLLLRWIHLLAGITWIGLLYYFNFVQGEWFKETDA
                     SAKTAAVQKLVPRALWWFRWSAMFTFLAGALILISKGMKGWEIYATSWGVVILTGSAL
                     GTFMFLNVWLIIWPKQQVVIASTNQVAEGGEALPDAAGCAAKAALASRTNTLFSIPML
                     LMMGAASHFPVGVTESTSFSGLFWVLAIIIGVLEINAVIGKPGPMASVKGVITSGFVL
                     TVVLFGVIGLLV"
     gene            17200..17577
                     /locus_tag="EYO68_08725"
     CDS             17200..17577
                     /locus_tag="EYO68_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIC07925.1"
                     /translation="MPRFCSILILFLLLTSCDLNIFKETDTDVEVVSEKNNETLTLGS
                     DPEKGKIVYYANCISCHNYNPKKPGSIGPQIYRSSKELLSKKINFGIYPKNYKPKRST
                     KIMPLQPHLDKEIANLHAFLNAP"
     gene            17840..18559
                     /locus_tag="EYO68_08730"
     CDS             17840..18559
                     /locus_tag="EYO68_08730"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIC07926.1"
                     /translation="MPVYQLIPEIPLFPPIEEAEDDGLLAVGGDLTKERLLGAYRRGI
                     FPWYEVGQPILWWSPDPRLVLFPDELKISRSLRKVLRKEQFEIRFDTAFQEVIKSCAD
                     VRTKQGEGTWIIPEMQQAYTELHQEGFAHSVESWLDGKLVGGLYGISMGQCFFGESMF
                     STMNDSSKVALVALADFSQRVGIKLIDCQMTTPHLLSLGAREIKREVFLKMLKKHLET
                     PSIKGLWNNGPVSMKVNLLQN"
     gene            18561..19280
                     /locus_tag="EYO68_08735"
     CDS             18561..19280
                     /locus_tag="EYO68_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIC07927.1"
                     /translation="MPESKGKKVFRIQLTVSVVVLLLTLSAFSLVAAFYLGMTTGISM
                     QRPPEGSVADTDISADKPLSEEELKFFGLSERKKDQDSFDMEELRDLKKKTTELTKAT
                     KTPDSASPKVPKTETPTVKAPDSPASEQKTVVKKSVPVPKTVVPKKPDNVKIPVVKSK
                     KDATYTIQVFASRKHANAKNLLEKLKQNGFKDAFIFKHTAGSKTLYRVRVGKINRSET
                     NNLANKLKKLKFIDSVQVTRF"
     gene            19392..20087
                     /locus_tag="EYO68_08740"
     CDS             19392..20087
                     /locus_tag="EYO68_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HIC07928.1"
                     /translation="MSNYWRFSRSAYYSVVAALPLLVAYEILVILTQSRYWSLRNAAD
                     MWIRTFLMAFDLQAQHLTFVMIGILFALIPLAKSYSTGVRLKVNYFALMLAEAFVYSL
                     LLGGLLQSILRVSGLSSGGPGNGPLQNFALSLGAGLFEEIIFRVLLLNLLFLLLKPFL
                     KSKVGVAVASVLVASFLFSISHYIGTMADQWQWYSFMFRWVAGLLFTVLYFMRGFAIT
                     AYTHALYDIWVLV"
     gene            20181..21341
                     /locus_tag="EYO68_08745"
     CDS             20181..21341
                     /locus_tag="EYO68_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIC07929.1"
                     /translation="MKVPLLDLRPPLEDLRDEIVEAITQVIDSTRYIMGPEIDSLEKE
                     IAEYCGTADAVGVSSGTDALLLSLMVLDVGPGDLVLTSNFSFFATAGVVARLNATPVF
                     VDIDPETYNIDPECVRMTLAEMDKETRKRVKAIIPVHLYGQCADMKAILNIAAEFEIP
                     VIEDGAQAIGAECEIDGKKRPAGSLGDFGCFSFFPSKNLGGVGDGGIVTVNEPELAEK
                     LRLKRVHGGERKYYHRVIGGNFRLDPIQATVIRVKLPHLNHWHNQRQDNAEYYNKLFA
                     ETSLGGSISIPQVMHSKRLQNPHIYNQYVIRAEQRDQLQSFLAENEISAEVYYPLPFH
                     LQECFLDLGGKAGDFPVSETAAEEVLALPVYPGLSRQMQEAVVEKIEEFYQA"
     gene            complement(21338..23446)
                     /locus_tag="EYO68_08750"
     CDS             complement(21338..23446)
                     /locus_tag="EYO68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIC07930.1"
                     /translation="MNPASQKVLENTRNIGISAHIDSGKTTLTERVLFYAGKIHKIEE
                     VRGGGAGATMDHMELEKEKGITITSAATTVEWNDTKINIIDTPGHVDFTVEVERSLRV
                     LDGAIMILCGVAGIQSQSITVDRQMKRYRVPRLAFINKLDRMGANPHNGIKGIRDILD
                     LNAVAMQLPIGLEENHKGVVDLITMKAIYFDGEHGDDLRFEEIPDEMKSDAESYRAEM
                     LEAVSMFDDQMMEDLLEEKEIAEESIHAAVKKGVQSLELCPVYMGSAFKNKGVQSLLD
                     AVISYLPSPLEAASTKATDIKTQEEVLLSCDPDKPTVAMAFKLTEEQFGQLTYTRVYQ
                     GKLSKGDQVTNSRTVNKMRVGRMVRMHSNDRENIDVAEAGDIIAMVGVDCASGDTFCG
                     EGIRVSCESIFVPDAVISLAVKGKDNEQHMKLSKALGRFMREDPTFHVSSDEESGDTI
                     ISGMGELHLDIYIERMRREFEVDVIVGAPQVNYREAITTAADYDYLHKKQTGGSGQFA
                     GVTGSIEPLPLDHEEGFEFENKIFGGSIPSEHIGACEKGFKDVMEKGPLAAFPMVNIK
                     VTLNEGRYHDVDSSDLAYQLASRYAMRQAVAKANPVLLEPVMKVEVETPSEYQGSVIG
                     DLSSRRGVIYGSDVKGDDTVINSGVPLAEMFGYATTLRSMSAGKANYTMEFEKYAECP
                     SFVQEKVIKERQEKLKEKEG"
     gene            complement(23587..24549)
                     /gene="selD"
                     /locus_tag="EYO68_08755"
     CDS             complement(23587..24549)
                     /gene="selD"
                     /locus_tag="EYO68_08755"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIC07931.1"
                     /translation="MLASLPQNNDPNVLVGFDTSDDAGVYLLNDEQALVFTADFITPP
                     VDDPYLFGQIAAANSLSDVYAMGGKPLSCLNLVGFPSGKLEDEVLERIVAGALQKITE
                     AGAVLLGGHTTDDDEPKFGLTVTGLVHPEKIWRNIGAQPGDQLILTKAIGSGVLLNAN
                     LKNLVSKEALQDCLDSLIELNKAAAEVLANYEIHAATDITGFGFAGHCMEMVPGSDLT
                     LNITLDDIPILSEAREMYERGVSTGVNETNRSLVEKYWKFPEGSAPILQELMLDPQTS
                     GGLLVAVPEDETTPILKDLHNVGVFPSACIGYVSNFSEAKLIFT"
     gene            complement(24781..25197)
                     /locus_tag="EYO68_08760"
     CDS             complement(24781..25197)
                     /locus_tag="EYO68_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIC07932.1"
                     /translation="MLKYTHTIQISWGHCDPAKIVFYPNYFIWFDQSAHLLFDKAGAN
                     MGDLMKQYGVVGLPIVDAHAEFICPSKYGDVIEVTSWISEWRAKTMIASHEIHNNGQL
                     CVKGTEVRVWAKPHPSNPKRLQAQVIPDSIRACFDV"
     gene            25464..26149
                     /locus_tag="EYO68_08765"
                     /pseudo
     CDS             25464..26149
                     /locus_tag="EYO68_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051992.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
     gene            26253..26732
                     /locus_tag="EYO68_08770"
     CDS             26253..26732
                     /locus_tag="EYO68_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07933.1"
                     /translation="MPFEQHFFQHSTKMKMINISQKTSWLVIFSGIIFFGSGCSKLVG
                     FTKENTTPTSCADRAIERAFNLHEGAKTGLALYFEERSDNQLYLAFYAATDSVYESRR
                     VKKCWDRRVSHYNAMQNLWEMNTALARVIRRNMPDDDRGELISVFREQYDWVMPNFR"
     gene            26848..27327
                     /gene="greA"
                     /locus_tag="EYO68_08775"
     CDS             26848..27327
                     /gene="greA"
                     /locus_tag="EYO68_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIC07934.1"
                     /translation="MSESYPMTIQGYENLKNELKELTSLDRPAVVNAISTAREHGDLK
                     ENAEYHAAKEQQGFIEGRIQELNAKLALAYVIDITKLSGEKIVFGATVTFVDVDTDKE
                     KIYQLVGDDESDLEQNKLSISSPIARALIGKSVGDTLTIPIPKGKIEVEILEIEFIV"
     gene            complement(27330..28262)
                     /locus_tag="EYO68_08780"
     CDS             complement(27330..28262)
                     /locus_tag="EYO68_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q synthetase"
                     /protein_id="HIC07935.1"
                     /translation="MNSPVISRLAPTPSGYLHLGNAFNFLLTALLVDFQDGHLHLRID
                     DLDDPRVERSAVEDIFVQLEWLGIKYDSGPSGPDELFSKFSQRLRMELYHESLELLRK
                     SGHLFACECSRSEIRQISSNGNYPGTCRNKKLNLLKGNQTWRILVPVQTLVCLKTFTN
                     KIEKIDVSREMADFVIKRRDGLPAYQLASIMDDLELGVNLVVRGYDLRSSTGAQLYLA
                     KCLNDSIFPETRFVHHVLQTDDAGDKLSKSKGAHSIQMLREKHRNPTWIYQETAKSLN
                     LPFEEIQTLEDLKEVFRSIMILKDGPNSILDHKN"
     gene            28719..29279
                     /gene="efp"
                     /locus_tag="EYO68_08785"
     CDS             28719..29279
                     /gene="efp"
                     /locus_tag="EYO68_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIC07936.1"
                     /translation="MYETSDIRKGLRVELDGGPFVVVDFQFVKPGKGTAFTRTKIRNM
                     INGAVIDRTFKSGEKLKPADTEDREMQFLYRDGEFHFMDNSNYEQVSLESSVVGDASN
                     YLTENMMIEVSFYKGRSIGLTLPNFVVLEVTETAPGEKGNTVTGASKPAMVSTGYTVN
                     VPLFVNEGDQLRIDTRTGEYVERVKA"
     gene            29349..30644
                     /gene="genX"
                     /locus_tag="EYO68_08790"
     CDS             29349..30644
                     /gene="genX"
                     /locus_tag="EYO68_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase GenX"
                     /protein_id="HIC07937.1"
                     /translation="MSGSSKIFGNSLLSGNYNGRVIEVSFETEQSQGSSPEYLYSILV
                     LVEDQLLPLSFREKEKIKNGALISFGVTEESEIEIVKIKNHAFNFDAESDLLRWRRPA
                     NNPSRMELLRTRQRILRGIREWFEEQNFLETETPILVRAPSPEAQFFPEKTSSGYLIT
                     SPEFQMKRLLSGGFERIFQFARCFRKNESGPLHNPEFTMLEWYRTNEPLAKLMSDIEQ
                     MVKHLTETVSAQSFPEQVPLPPWPRVTVSELFKKHLDIILDGSENADRLREKAKLAGF
                     YESKAEFNPASKLTESLDYEQMFFKLWNHFEADLGIVSPIFVYSWPLPLASLARHCPE
                     HPGFADRVELYADGMELANGFGELTDASEQRRRFEQDLENRKAEGRSGVPLDENLLKS
                     LEQGLPESSGMALGVDRLIMWLCGVQNIRDVICFSEDEI"
     gene            30701..31012
                     /locus_tag="EYO68_08795"
     CDS             30701..31012
                     /locus_tag="EYO68_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07938.1"
                     /translation="MERSIIHIILHFLVPFIVAKTAWRELWLWPFIIMALTFVIDFDH
                     FLAEPVFDPNRCSLGFHPLHSWPAIGIYLVSLFSPRLRIIAIGLLIHLVLDGTDCFWT
                     D"
     gene            31255..31467
                     /locus_tag="EYO68_08800"
     CDS             31255..31467
                     /locus_tag="EYO68_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07939.1"
                     /translation="MAKKAKNISHEEVSEAMARFLKKGGVIEKIEYNDNGFLLKNDLA
                     LDDSYTETSDSIMSQLHIDFETETRI"
     gene            complement(31656..32072)
                     /locus_tag="EYO68_08805"
     CDS             complement(31656..32072)
                     /locus_tag="EYO68_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIC07940.1"
                     /translation="MSISRDELVNIFSVVSFLAHAERDVHVAEKKVLIAVFKAAGITQ
                     EEQKKMKSNTSLEEMLKHIQSVETKHTLVELMALVAASDGVFEDEERVIIKKIMKRIG
                     VADDHPYFDDNNLDVPTVRANVGKILNSIKTLAPKP"
     gene            32215..32631
                     /locus_tag="EYO68_08810"
     CDS             32215..32631
                     /locus_tag="EYO68_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIC07941.1"
                     /translation="MGRDTRYHPEWSTVSRYVRELFNYYCSRCGKDCRNTKNAEMVLQ
                     VHHIDENPGNNDLENLIPLCASCHLKIEREARLHAPNHEKQLELFENQSYMSQMKMMR
                     QSALVKHGAVNKADVSQMDAETYEIRALDWEINEPS"
     gene            complement(32682..33785)
                     /locus_tag="EYO68_08815"
     CDS             complement(32682..33785)
                     /locus_tag="EYO68_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07942.1"
                     /translation="MGKKDNEPSDGMEKLFSTQGILIAILILGFYWVFFGEDNNRESR
                     GFNFTTQNVPIGVSDQLNPEALEKLGINLTEHLDPKLIPAITSEAIRETTQENVSKDS
                     FLPILVSKISNKIRDVSTIDLNEDGIADPVLIVPQTETADSDFLVFSILVPDPDKVNV
                     LPSVSDNGAWREIAENKSIEIMTASVVRGSGDQMTMQSAPNPQMYQSAGAPYPPYYGT
                     GYSMTNLFMTSMMMSMLFRPPFMWGGGYGYGYGGGPMAVSSVQNRRGAATSSLKKASP
                     SKSAAKTGSGRSVSSNKFRSTSKQSLNNIKSTKFRSANRKASGGGFGNSRTQRKPVAT
                     QRRRSSTRRRSLFGGGSRRMRGFGFGGFGRRRR"
     gene            complement(33894..34826)
                     /locus_tag="EYO68_08820"
     CDS             complement(33894..34826)
                     /locus_tag="EYO68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HIC07943.1"
                     /translation="MTLIVRPDYHAVPILERNGIRWIPPSQAKRQDIRPLRIGILNIM
                     PLGEKYEFNILHPLGLSVLQLEPIWIRLESHNYKSWEPKHVDDIYVTYEEAMRDQPLD
                     GLILTGAPVETIDYEDVYYWEEIKTILSDARKNIPSTLGLCWAGFVMAYLEGVKKLNY
                     DHKLFGVFELKNLAPDHPIIGELDDVFFCPQSRHAGMPDEAMEEASESGRLKLLAYGP
                     EAGYSIFSTTDDRFIAHTGHPEYNATRLAEEAKRDHGNPEVPAPVNFDFNNPINRWRS
                     HRNTFFAQWVSYCYLKISTHDMAVGKNLIASNTA"
     regulatory      complement(35035..35149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(35221..38679)
                     /locus_tag="EYO68_08825"
     CDS             complement(35221..38679)
                     /locus_tag="EYO68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-PII uridylyltransferase"
                     /protein_id="HIC07944.1"
                     /translation="METNFSPIENYPFLSPFIFTENPEELEVHKEALLKQLEEVWRPL
                     AIDSCQSMEYLTAREKVFAGVIEEYYREQYKKIVESSLCTNNSFDTLSKNTHLLDSII
                     HTAFEYGFADLQILKERIKEDFKKELLFKKRSLPKKKKKLSLSRTQIEKVESNPEEPD
                     QRQMLKYYESIEAELIHEIENLSERLKELEELLPQVQKSEIKLNLLLNHLVVFARGGY
                     GRAELSFASDRDLGYCLDTQQLSAGESEICRQFIIHIEHLLREAGIETAHQYFELNED
                     LSRFKDPSVIHTIPSILESRVLIGSKDLANALKRRFFQILPYETFVLSQIRDYNDRTV
                     PDLSQMNLKEDRGGLRSLQIPLWLSAATFGIFPSQTAEMLALLIQKRIISPRQGYKLC
                     QALEFLYDLRNFSASAKEYHFDDEARESGLSEKDIQSNIINDATERLYLLKKKRFQSI
                     DDFDRYRLQMVNHIQDLSQAILQRLLDRTIVRTFSNFQVVVHLGKRLIVEVNALEGLP
                     QVPISLIFNDPTALLELFEYVGQSEYDLSFELKDEMADLIRIITPDVISSNRTQIAKS
                     FTKLMLTPFTANAWRIMLEICEPINAESQPRTLIGCFIPETNKMRFLLRNLAYHQHPV
                     CVHTLNALDRTQKELDRLKKDYQELYQYLEPKHILALKWGILFHDVGKIDPQTDHEVS
                     GTSIAVNALERIGYDDQELFTLVSLLIVHHTTVVQLSRTSAYFDQALQSFFEIADRNL
                     INVILLFLCNISDYISVSESNAHSTRGLRTFFEETYRVFAEMRSSKLQEDSMDFIQTY
                     LDIKKNDLESDTRIDLLINRSLRENIESVLLTPLKKINKEERKLLGKSEDDLQVLWRD
                     LKLGSLDKQGTDQTTDKFIRTIRQSISKKSLVTLTELYSPMINWFFAAFPNRFLLSST
                     PDMLAENLSIFNRLERPAIVNVITNARGRLNGLLIYVHDQLQIHSRIAYTLNLKHLNI
                     ESAKINQIQFASGKVAFCYYLKVSKREEQNVILPRELETSIKSNTLPALIIKTQTFLY
                     NTKLQLEYLKDDKKGYMVKEKKSEALGDFPVWNGEFREKTDFSRRDKNYLRIKITADD
                     APLVYYKMVSAFDQVGVPIQQAVITTIGHQVIDTFYITPEDHEKLVKSNFEESLKESL
                     MSPSEI"
     gene            complement(38833..39897)
                     /gene="ruvB"
                     /locus_tag="EYO68_08830"
     CDS             complement(38833..39897)
                     /gene="ruvB"
                     /locus_tag="EYO68_08830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIC07945.1"
                     /translation="MQDQTVDTETSVSKENSEDRLVAPESRLGEQFDQSLRPQRLQDY
                     IGQNEIKENLHVFIGAAIKRGHALDHVLLSGPPGLGKTTLANIFAREMEVQLRSTSGP
                     VIERQGDLAAILTNLDPGSILFIDEIHRLNRVVEEVLYGAMEDFTLDIIIGEGPGART
                     VKIDLPHFTLVGATTRAGLLSSPLRERFGIQFRLNFYDAEDLKTIILRAASLLGIEIV
                     EEASLELARRARGTPRIANRLLKRCRDFADMETSGVITLPLVERSLERMRIDLEGLDQ
                     MDRHILETIIKKFGGGPVGLSTLSAAVSDEKDTIEDVYEPFLLLKGFLQRTPRGRVAT
                     ERAYQHLEISAPPQSQVDLF"
     gene            complement(39907..41400)
                     /locus_tag="EYO68_08835"
     CDS             complement(39907..41400)
                     /locus_tag="EYO68_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HIC07946.1"
                     /translation="MSKPKFLSTNVAALLVYGRPPMVFAGMICAIGVMLDHNPLVYYS
                     GVIFLLAAMILDIIDGWFAARFRPQAKLAHLADRIMDKVVYAIVFPMVAVGMMWRYQY
                     LPESADFRLEMLHVVFVFVLCVTVLMRDNFAHFMRNFSLRKGEEEEMKEVTRLRTMVA
                     APVGVVLYIHAFYVPGGPDSSLYSWISWLGAIPIQQLFFLEILFLIINFGSIAGYCRK
                     YGTACLDDLCLNDEVLRRRILAVFPNVLTVMNALMGVLAILFAYRGRVQEAYLILLGA
                     GFFDKIDGAVARKLGLTTPLPSAKPKKYNITLGGVLDDVSDTVSFCIAPAVIFYMLMG
                     RVADESIQSLPYGWIAILYVVLGITRLVFFILDQNSIPGFFKGIPVPGAALLVAAPFI
                     MIGNALESNTPDLVFWSKFSFFLMIIAAILMISFPIRYMHIGRLMSRSRKFLIFTIVL
                     VIGFVFTPYFGHAALGYLILYVFSPLYTWRISPDIASQEHLEKLSTS"
     gene            complement(41472..42470)
                     /locus_tag="EYO68_08840"
     CDS             complement(41472..42470)
                     /locus_tag="EYO68_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIC07947.1"
                     /translation="MQHYEKDGRLQHVMLSHQFSIKLMEELFDIADHVKGMTRKPNGI
                     EFLKSLLSHKKAMLYFTQPSTRTFLSFLTACQMVGMDTGEVRDPSLSSEYKGESQEDG
                     VRVFSSYFDLIIMRDPKPGFCEYMAYLLDNTGRSVPIMNGGSGKDQHPTQALLDLYTM
                     HRSFQHKGGISKKRYAFVGDLLRGRAVRSSVMLLSQYKDVEMDFVAPSSFQIAEDLED
                     ILRSQNIKIRKTDDLGSILSDVDCVYMTRLQDEYDLSGESNLIDYSQFSLTTDNVNQM
                     KSDAIILHPLPRRHEISTEVDADPRAMYWRQLRNGVYIRAALLLYVFNVAHRLKDY"
     gene            complement(42753..43136)
                     /locus_tag="EYO68_08845"
     CDS             complement(42753..43136)
                     /locus_tag="EYO68_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIC07948.1"
                     /translation="MADITKDQVVDFIANMSVLEMSELVKEMEEKFGVSAAAAAVAAP
                     VAGGGESEAAAEEKTEFDVILTSFGEKKINVIKEVRSITGLGLKEAKEAVESAPKAIR
                     EGVSKEEAEEVKKKLEEAGASTEIK"
     gene            complement(43210..43734)
                     /locus_tag="EYO68_08850"
     CDS             complement(43210..43734)
                     /locus_tag="EYO68_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIC07949.1"
                     /translation="MERSQKEAKVSELRSIFNNMSAGVLVDYRGIEANQVVELRKQLN
                     DASSTMKVLKNSLARIAAEDTPFSELADQFTQTRALVYSDGDPVVQAKVLSEAAKNID
                     NLKILAGILVGDSKTSILNTGEVEALSKLPSREELIVKLLFLMQAPATQFVRTLNAVP
                     AKFVRTLAAIRDSK"
     gene            complement(43997..44686)
                     /locus_tag="EYO68_08855"
     CDS             complement(43997..44686)
                     /locus_tag="EYO68_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIC07950.1"
                     /translation="MSKQSKRAKFIQDKVVSEQEYTLSEAVNLLKEVKATKFDESVDV
                     ALRLGVDTRKAEQMVRGTCSMPNGLGKEVRVLVFAKGEKAIAAQEAGADYVGGEDFAK
                     KIQDGWLEFDRVVATPDMMAVVGRIGKILGPRGLMPNPKTGTVTFEVESVIKNIKAGQ
                     VEFRADKLGNLHASVGKISFDADKLCENISVLVDTVKRMKPSSSKGIYLRNFSLSSTM
                     GPGIKVDPQTV"
     gene            complement(44683..45108)
                     /gene="rplK"
                     /locus_tag="EYO68_08860"
     CDS             complement(44683..45108)
                     /gene="rplK"
                     /locus_tag="EYO68_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIC07951.1"
                     /translation="MAKDISSFIKLQIPAGKANPSPPIGPALGQHGVNIMEFCKAFNA
                     QTQGQDTIIPVVITVYNDRSFTFITKTPPAPVLIKKALKLNKGSATPHESKVGTLSKA
                     QIEDIAKQKMPDLNCYNLEAAVKSVAGTARSMGVLTEEI"
     gene            complement(45120..45938)
                     /gene="nusG"
                     /locus_tag="EYO68_08865"
     CDS             complement(45120..45938)
                     /gene="nusG"
                     /locus_tag="EYO68_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIC07952.1"
                     /translation="MVEEQTGTEMLFEEQEKNTETQEINSDDQEDNLSEETDVDLEVQ
                     ENAPQISDAYLGEQEDAATTEMVETVENSMETENQTEETESQVAEKPQNPNVKWYILQ
                     AYSGYEGRVEQTIREKLRIKGIEHLVDEIFIPSEDITRTKEGKKRKVNQKYFPGYVLI
                     HMELTPELWHLLMNVNRVSGFIGGTKKKPLPLDEKELEEIRSQVNEGFQQKATEDEYS
                     VGQKVTITEGSFGNFSGTIDEVNLERRKLKVLVSIFGRPTPVEVEFENVKHVVE"
     assembly_gap    45968..46067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(46095..46283)
                     /gene="secE"
                     /locus_tag="EYO68_08870"
     CDS             complement(46095..46283)
                     /gene="secE"
                     /locus_tag="EYO68_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIC07953.1"
                     /translation="MFKRIRQFFKDVIAEFKRVQWPTRDATIKSTSVVVCLSFAVALY
                     LGIADLGLSDIMQMIIAG"
     gene            complement(46380..46455)
                     /locus_tag="EYO68_08875"
     tRNA            complement(46380..46455)
                     /locus_tag="EYO68_08875"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(46420..46422),aa:Trp,seq:cca)
     gene            complement(46487..46636)
                     /gene="rpmG"
                     /locus_tag="EYO68_08880"
     CDS             complement(46487..46636)
                     /gene="rpmG"
                     /locus_tag="EYO68_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIC07954.1"
                     /translation="MRDTIHLECTECKRRNYTTSRNKKLKTERMEVRKYCRWDRKHTL
                     HKETK"
     gene            complement(46671..46745)
                     /locus_tag="EYO68_08885"
     tRNA            complement(46671..46745)
                     /locus_tag="EYO68_08885"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(46711..46713),aa:Thr,seq:ggt)
     gene            complement(46780..46864)
                     /locus_tag="EYO68_08890"
     tRNA            complement(46780..46864)
                     /locus_tag="EYO68_08890"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(46828..46830),aa:Tyr,seq:gta)
     gene            complement(46869..46944)
                     /locus_tag="EYO68_08895"
     tRNA            complement(46869..46944)
                     /locus_tag="EYO68_08895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(46909..46911),aa:Thr,seq:tgt)
     gene            complement(47076..>47207)
                     /locus_tag="EYO68_08900"
     CDS             complement(47076..>47207)
                     /locus_tag="EYO68_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIC07955.1"
                     /translation="EDGKYQLLSAEEVFQDYQFSKNHDIKLPDRKKSADSKIYLAAA"
BASE COUNT        13695 a         9661 c         9405 g        14346 t
ORIGIN      
        1 tttttgattt tgaaactggt aaatcctgag atttggcaat tgcgtctgta accggcgtct
       61 ctggttttgg caaactctct attaattgga ggtctgattc aggaaaatca acactgtcca
      121 gcaattcaaa tttgagaata aattgaggtg agagcatggc ttcagtttca gtaggacttg
      181 cctctttgag ataatcttcg tctccagcaa aactattacc ggaaaaaaat acccactgac
      241 cttcattaaa attagccagc cagctatgca gtgatgttcc gggaaggatc agagtgtcgt
      301 aatccaaatc tgtgtgggta ttgttccagg ttcccattgt gaccctgaat tccggagcag
      361 ttttattttc agtagcattt tccggaggaa ccattgcgag ttcaatttcc aaataagctt
      421 taccattacc agtagtaagt agagtacgaa tacgaccgat ccactttgtt aagaccctgt
      481 cctttagttg ctcagccaaa aattgttgtc gtttttgtcg cagtaaaaat ttttgaacag
      541 cattgggtga ggcttggtat ttggcagcat attctgctaa accattcatg aattgttgct
      601 gaatttccgg aatcattgct ccaagtattt ccggagatgg attaatgttt ttgacagtat
      661 ttttcggttg ctcactccaa actgcagtcg caaaaacaac acagcatatc agcaaacata
      721 ttccaaaata ttctctatga ttcagcattg tctctttttt tcgatctaat gtaatccgga
      781 tccttcatta actatcaaac aacatagcag tgtctgaaaa cacaacttca atcttgagtt
      841 catactcact gtttttctcc ctttttgtta aactggacaa gaaagatgtc tttcccacca
      901 ctgtattttg ctccatccat tcgaccttct gtaaaacctg taacgtatat ttgtccttcc
      961 gttccaatag ctaaacctaa ggggcgttcg ttttccggtg taccaaattg ccgaagccag
     1021 agcaattcgc ctcccgaatc cagcattgct aaaaaagcat cctcctttcc gaaatgagat
     1081 tgcttttcca tactactttc agtatagcct cccaccagca agttcccttc tggagtccac
     1141 aaagtaaccg tacattgttc agatttcgtg cctttgaaaa tatatgtcca taattttgta
     1201 ccttcggaat tatattttgc gacaaatgca tcataatttt tttgcatatc tgtaccatta
     1261 ctttcagttt gagtagtgta cccaaccaac gcaactgctt catcagatag aattgccagt
     1321 gaagtccccg agtcatcgag ctttgttcca aatgttttgc tccagcgttt aggccccatc
     1381 ttatacattt tcagcagaaa aacatctata cctcctgttg atttattccc gtcaagagac
     1441 cgcatcatac ttccggtaag atacagacca gttcctgaat cagcggcaat tgcctgaaga
     1501 ctgatccctg gatttaattc agtcgcccac attttttcgc cggttttgcg gatcttcatt
     1561 aaattgaaag gagatgcggc agaaacgtaa atatgtcctt ccgaatccaa tgccattcca
     1621 tttcctgata ctgttccaga aattccgatt acttttgaag ataatttttt gccatttaca
     1681 tcataagtca acttaataac agcagaattg ttttttccct gcgaaagacc actcaaacta
     1741 agttgacctg attttttcat tcgcaaaact gcagccttaa ctgagcgtcc aaaaccaggt
     1801 tgatgaatcc aattcaagaa accgcttggg gcatattttg ccagaaataa atctgattct
     1861 tctccacttt cacttacact ggtgtttcct gcaagataga cattaccatc cttatcaaca
     1921 gtaattgcaa aaccggtatc gtcaccttgc gtcccaattt gctggctcca tgtcgtgcga
     1981 aatccaagtt tagttgtaag attttttttc aatgaagcac catttatagc ttttatttca
     2041 gtactcaaac gtatttgatg aaactcttgt gcagcataaa ctcctttggg agacagcacg
     2101 attcttttat taccgtcctc taattccggt agattcatgc gcacacaatt tctgaaatta
     2161 taggagctaa tttgtatggt accggaacat tgcgtatctt cagtgttgac agtaagcgtc
     2221 agaggatcaa ttggaacact aaatttcaaa acaatttttt catttcgttt aacagtgatt
     2281 gcattgtctc tcggttctat cgaaacaagt tttggagtta taagtgaagt gttttttggt
     2341 tttttcacaa caacatttgc tgttccgccg caccctccca ataatacatt cattagaaag
     2401 cttacaaata caactgaaca agtaattttg gttgttaaat tcaatttatt aattttattc
     2461 ttcatctgtg aaaaccatga catttatctg gttcctacta tgcgatttta cagcaagcat
     2521 gtcaatgtct gaggcacttg aattaattgt agaattacaa agatacagaa ttaaatgaga
     2581 aaatatgtta gaaataaatg ctacacagaa tgacacttta tcaaaaatcc gcgtgtttat
     2641 ttctcataca tttcgctgga ggcgatggct gttactttgg ctaatgttct ctgcgtccag
     2701 catacaagct cagcttataa cacagagttc aggacatgag cttgctgatg aaaaaactgt
     2761 ttttttgtca ctctggaaac aggctgacga gcgccaactt actcttcatc cttattggct
     2821 gaaactttta cacttttatt catttggtga atcagtcggc caatggagtt ttaaaagtga
     2881 tatagtcagt agcggctttt ttctttcaca agaaggaaaa atcgatccga cagccgaact
     2941 taaagcaaca ttaaaagcat tgctttcacc gctcagtggg gaccccaatc tacatgcacg
     3001 gtgtaagttt attgcccgct acaactggct ccgcagtaat cttgattttc cggaattacc
     3061 aaaactgtcc tgccctctat ttgaacgttg gtcgaatcta gaagaagcaa ctggaatcag
     3121 catagtattt gtctcatctt atttaaaaaa tccggcttca acatttggac atctactcct
     3181 caaattcaat tcgcaaaacc gttattttgg acattctctc ctcaggccca cattgaattt
     3241 tggggcaatg attaatcctg atgacaaccc tttaatctat gctttacgag gactatttgg
     3301 ggggtacgac ggaagattta ctgatgaacg attttataat tttaatcatg tttatggaga
     3361 aaatgaattg cgtgatttgt gggagtaccc actatatttt acagttgaac agcagcatcg
     3421 tgtggcctat catgcatggg aattgctaca aaacgtaaac tttacttatt attttttcct
     3481 cgataattgt gcatatcgaa tggcagaatt attggaaatg gcgtggaccg acacaactcg
     3541 aatcaatact tccggagcaa tttgggctat tccggtcgat gtagtgttta aactaaaaaa
     3601 tatcaaaaaa gaatcaggag aacaatctct actaggggat ccaaagctga ttccgtcaag
     3661 acaaagaaaa ttgcagaacc gcacagctca attaagtgaa atggagcaaa aacaattgac
     3721 gaaattgatt gaatctgaaa attatttgga ttcagaagaa tttcgcagct ttccggaaca
     3781 gtcacgagca agaattattg atactttgct ggactacagg cagtatgtga aaagggacaa
     3841 acccacactg aatcagcaaa aagagcgttc tgtactgctc cgtgtccgta gtgaactgcc
     3901 aattctggaa aacaacgttt cagcagaaat tcctgaagcc cccactgacg gtacaccacc
     3961 tatgcgtttt cgatttggtg cagtttccaa ctcgctattg ggacctgcac tggaagttgg
     4021 tacctgggca aattatcatg atttacttgg agatgaatca ggacacctgc aaaatgcaga
     4081 agttgtaacg ctggatttgc atctgcagtt acgcaaaaac tcttttgaag taacacaatt
     4141 ccaattattt aatattcaaa aatttgccct caatccaaca ggaatatccg gagattatga
     4201 atggtcatgg cgtgcgcgtg gaggttggga acgagaacat cttggttgct taccctgcag
     4261 aaaatttagc atagctggag gttttggcag gtcagtttca attggtggta aagacttgga
     4321 gtaccttttt ttagatctct ttggagaaac aaaaaatcat tcgtggccgg caacaacttg
     4381 gggttatgca ccacatcttg gtatgttgtg gagtgtattg gatatttgga aaataaggtt
     4441 cgacggaggt tggtttaaaa gcatttacgg tccaaaaata gaatatttcc acattcgatt
     4501 tgatcaacgt ctgaccattg ctcaagactg ggatatccgg atggaaatag aacagcttgg
     4561 aagtctggaa ggaacattag ctttacatta tttctggtga gttataaaaa caaatgttga
     4621 attttgacat tttaagggca caccaaaaat gaataaaata cgagagtgtt tatttacttt
     4681 ttacaagttc atcataaatg aacggctgcc gctcattcat ctctctgcac ttctcattca
     4741 tgccgtaaca acacgggctg tttaagattt aattcccctt ttgaaacatt gtatatgagg
     4801 ccgcttccat gcaaagtgtg tccttcccgt aataccctca ccggtttggg gatagtaaga
     4861 atctcttgtt ttgcattata gattgcagtt tctgtgtgaa cttcagagcc gtcattccgc
     4921 aaaagtacta cattcccagt aagtgttacc ctgttatcgt cccactctat caagccctgt
     4981 tctgcagtaa taaaaagatc aacctcctga gtaggaaggt tggttgatag aggcaaattt
     5041 ggtgaaggtt tgacagaaga attgtctgaa acaaataccc agatcgttac ctgctcaata
     5101 cgcatacgac tggatttttc cgaaataacg gcccttttgc ctttgagctt ccaacttctc
     5161 cgctccaatt ggtaatcatt tactgtgatc tgctccagat ccaacacacc tctttcttca
     5221 aaaggaatat tttcaggtgg ttcgctgaag gaaaataaat aataggcagc tccggctagt
     5281 gctcccaaca aaagaagaat cgtgagttct ttgcgatttt catgtagaaa ggacgaatag
     5341 gtctgaagtc cctgagattc catgataagt tgaagaaata ttattcagaa acggaatgcg
     5401 aaaattggaa cctcccactt taaaggagca ggttttactg cacgagaata tagcggcatc
     5461 atctcagatt caatgaaagc tgtatttggt tgttgtgtac tagattgcgg agatggtagt
     5521 tcaagaagat aggatttgtc aaaacttaag cctcctacaa ccagatatat ccctagtccg
     5581 tagccaagta ctccaccaag tgttgtagaa gtaatcaatg actctctgag ctgaattcct
     5641 gaaactaaat gataagaaaa acccatagtg gctccccatg ctgagcccca aagggtgttc
     5701 caaaaaacag agtacattac cggttttttg gggccaccaa gtggaatgac ctcttcctgt
     5761 gctgaaatca caggaagatt tatgcacccc agtaaaacaa ctataacaaa tattcttttc
     5821 agccttagtc tcatgtacct agtaaaaata aatatttaca gtatcctaaa atcggaatcc
     5881 aaaaataggg agcctgaaat tttgaacaga tttgatttca ttttgagaaa taattgaact
     5941 ttgaatcggc cgtaaaaggt cgtcttttcc caggagttga tccaaatttt ccggcaaggt
     6001 tgattctgag ggtactatga ttgcgttctg cagcatataa aatccaaata atgcacccaa
     6061 tgctgttccg gcaatgaaac cgtttttaac cgtacttttc aaggtgacgc ttggattcag
     6121 gggatccatg aaccagacaa caacacctaa acctgctccg aagacacctc cgccaagggt
     6181 gtaccccaac tcctgcttca tcattaaagg tcctgtttcc tgtgcctgca aagaagcagt
     6241 gatcatcaat agaaaaaaga aaggtaaaat ccattttatt ttccagagtc tgagtttttt
     6301 cattattgaa aagcagtcag gatttatatt ttgagtaatg tccgttacag gtcattccat
     6361 cgagtgaatt ggtcggtttt tttaacaaca aatgcaggaa tatgaatata ataacctact
     6421 aactaaaaaa agagttaaag acggtttttc tgagtttgaa tgagaattgc atttaggcta
     6481 ctgtgaaatt agctgaaaag tctaagtcta tttcaaattt taatcttttt caaaattatt
     6541 agtctctttt tcatgttctt ctttaccgcc attcgacggt ttgttttagt tttaacagtc
     6601 cttttttgtg tagtatcgcc tgtagttgat ctagcagcac aggagtttga cgatgatcct
     6661 ctcatgttag atggaggcga tgaattcggt tttgggcagt tcggcgacaa tgacttccaa
     6721 agcggtgatt ttggttccgg agggtttcaa gattttggaa cccagcagac acaagaccct
     6781 caagatcctt tcgactctca gtttgattca actgatcaat atcttgatga aggtgcgttt
     6841 tctgatgaaa gtgaatcaac tttgaaagac gatctgattg tccgtcgcga attattatca
     6901 caagaaggaa aagctgtccc ctctaactta ggttatggtg caggaaccgg gttgatgatt
     6961 ggcacctggt tcgcgtttat tcgaaaagaa accaacactc gtcagcaatt ccgcactata
     7021 ggcaccagta ctgttgtagg agcattgata gggattatgc taggtacacg tagtgtatgg
     7081 gatcccggtg cccctcgtcc agaggatgat gcttccccca cgggtttttc gcaatctcct
     7141 gcaggttggt tatttgccca gaataaagaa tcgttaaaaa ttgcctaccg ctggaagttt
     7201 tagcaacact tacgccaaat caaggtaaca cgtttcagtt tacagggtat agaaaacaat
     7261 atttgaactt gaaacctgaa aacttattac ttcacctgct gcgaattatt ttgtgcaact
     7321 gacgcatatt ttctcgaaaa cggtaactta tagtgttcag caaaattcca atccccatac
     7381 tgccaaaccc taacaacata aatccagttg ccaaaattgc agttgggacc ttgtttacat
     7441 attcatcttc cagatagtcc agaataaccc agattcctga gattcctcca gccaatataa
     7501 aagccagtcc aataccccca aaaaaagtaa aaggtttgta agctttcgca attctggcaa
     7561 tttctgcaag caccctaaaa ccgtcatgga aagttcgcaa ttttgaaaca gatccctccg
     7621 gacgctcccg gtatggtaat ggaacttctt taatccggaa tccataatcc agtatccgaa
     7681 ttgtcatttc tgtttccact tcaaatccgg aggaaagaat cggaatggat tgaaccagtt
     7741 cccgactata tgcacgatag ccggacataa tatcggagag gttgctttta aaaatccagt
     7801 tgaccagtct gcgtaccaga ttgttgccaa aaacatggag cggccggaaa gacgttgcgg
     7861 tgtattctgc taaacgcaca gcaactgcca tatctgcttg ttctttcatc accggttcca
     7921 gcagtttccg gacatgctct gcagaatatg tgtcatcacc gtcaactatc acataataat
     7981 ccgcatccac tttccggaac atacttgcaa ccacatgccc tttgccggga cgcgattccg
     8041 ggatcacttc tgctccacac tctcgtgcga ttgctgcggt gcggtctgta ctgttgttat
     8101 caaaaacaat gatttttgct tcaggaattg catcctgaaa atcatggatc actttgccaa
     8161 tagttatttc ttcattaagg caaggaatca gaatgacaat ctttgcttta ttgttaatat
     8221 ccataaattt cagaaaaaaa gtagttgctc aaagtttata tttttttttg atacttcgca
     8281 actcttgttc aatatcagtg tctttaatat ttgaacgaac agaaaatgca gattttgaag
     8341 ttgccttaac ttgttgtttc ttcaaacgct tcaattctgc ttcaatgttg tctaacggat
     8401 ctttatctgt atacgatttt gtctgcgttt tatggtttga actttctgta cttgttctat
     8461 aatctgcgtt atcttcaaag gactcagtca cttcctgaaa aatctgccgt gcccagtcca
     8521 caaatatttc cagcggatcc ctaaaactta atccgaattg tcccagcatt tctccagcgt
     8581 cccgtagtaa atcttctaaa gtgtcatcct gttccaaagt agcagattca tgagaattgc
     8641 cacgaaactc tggtttttgt ttgtaacctc ttctacttga agaagaactt gagcttgttc
     8701 tatttttctg gtcatgtggt tgtgaagctt ttgcaccaga ctttccaagt tgtcggtcca
     8761 gaatattacg tttttccgga tctgcaagaa cttcataggc ttgagaaaga cgtacgaaga
     8821 tcttttgttg gcgtttacgt tcttcttcgg acacttgctg aaataagtcc ggatgacatg
     8881 atttagcttt tctgcggaat gctctgcgaa tcagagaaat atctgcgtcc ggaggcactt
     8941 caaacaaggt gtagtaattt tccaattttc aacaaacaac taatgatttt gagctatgaa
     9001 tagaatcctt tattttgact tggaaaccaa gtattctgcc gatgaagttg gcggctgggg
     9061 taacatagaa gacatgggaa tgtctatagg tgtgatttgg gacacactcg atgaaaaatt
     9121 ccatgtctat attgaacacc aaattaagga aatggtcgat catttttatc gtgcagatca
     9181 aattgtagga ttcaaccatg ttggttttga ctatcgagta gtggccggag tccgctatgc
     9241 agactcccat gaacgttccc gtcttcacac agagttggcc ggactgaaaa attttgacat
     9301 gctgcttgaa ttgaaaaaac ttctcggtca ccgtctcaaa cttgaatcca ttgcacgccc
     9361 aacacttgga accggaaaat ctgcagatgg cctgcaggcc ctgaagtggt acaaagaggg
     9421 caaactggat aaaatcattg cgtattgtaa agtagatgtc gaagtaactc gtgatgtgca
     9481 tctttatgcc cttgaaaacg gtaaactgca ttatgattca cgaagcggca ttaaaacggt
     9541 aagtgttgac tggaacagcc aacccaaaaa acaggagcca cagcaaatgt cattgtttta
     9601 gtagttcaaa tttgctgttg tcagtactat ggtctccaaa acaaacttag agcatcttaa
     9661 ttaattttat gcttttgttt actaaatcat gacttttttt aaaaaactgg aaaaacgagt
     9721 tagtgccaga cagacctatc tttgtgttgg ccttgatcca gtctggggga aaatcccaga
     9781 gcatctgcct cattctcagg aaggtttact agaatttttg ttgcaaattg taaatgaaac
     9841 gaaagatcat gttgcagttt ttaaacctaa ttttgcctat tttgaagctt tagggccttc
     9901 cggaatggaa gtattgcagg aattaattca taaaattcct cctgaaattc ctgttatagg
     9961 tgacgcaaaa aggggggatg tagggcactc ttcacggatg tacgcaaagg cagtatttga
    10021 aacttttggt tgtgatgcgg taacagtaac accgtatctg ggacatgatg cattggaacc
    10081 gtttttagat aatccggatc gtggtgtttt cgttctgtgc ttaacatcta attctggagc
    10141 caacgacttt cagcttccgg atttacattt aaaggttgca aaaaaagtac gggaatggaa
    10201 tatttacggt aatgcaggct tagttgtggg agccacccga ccggaaaaca tagctgcaat
    10261 acgagagatt tccggacgga tgccatacct aattcccgga attggaacac agggagggga
    10321 tttaagaaat acgctttttt acgctgatga tggttcgaaa attccatatt tgatcagtac
    10381 ctctcgcagc atactgtatg cttcctcaaa agaggatttt gcacagcagg cagggactac
    10441 agcccggaaa ctgcaggatc aaatcaattt gtgtcgctca agtttgtgag catgctggta
    10501 aaccccccgg ataagaaact ctgaactgga acagcatttt cggtttgcag tttaacttgt
    10561 cccaacaatt tgccatccag ccgatattcg aggctgccta gttgttgttc aactttaaca
    10621 ggtgccgtga tctgtttggg gatctgaggc cagatttcca gtcgtttgtg ttcttcctga
    10681 ctcagtaaaa tggtgaccgc atcagttgtc tgtaaagaaa caccttccgc ttctccattt
    10741 tctacactta ctgaaaatgg aacagtatca cctttcttgt tcaactcata ttttaaataa
    10801 ttttcgaaac cataatcgag caggtttttg gtagccttgc tcctcaatct tgaatttttt
    10861 gctcctaaaa ctactgtaat atagcgttgt cctttccgct ttgcagtgga aaccagatta
    10921 aaaccggctc gccgatgata tcctgttttc attccatcca tgccgcgata attgcggatc
    10981 atccgatgat tggtgttcaa taattggaac gtcccgtttc tgaaagtatc cagacggatt
    11041 gaggaccaac taaggtacag tggatgcttt aagagttcca atgccagcaa atacaaatca
    11101 tatgcgctgc tcacatcaat tttttggccg cgtcctggtg gtagaccgtg tactgaatag
    11161 aaacgtgttt tcttcatgcc aagttcatcg gcacgttcgt tcattttttt gagaaaaaac
    11221 tttacttgcc cggaaacatg ctcagccaca gctacagctg catcatttgc cgatgaaatg
    11281 actgtggctt tcatcaactc tctgaactta aaaacttcac cttgctttaa ataaacctgg
    11341 tgtccgccaa ttttgctggt ccagcgagaa atatttacct tgtcctgcaa actgattcgt
    11401 ccggattcaa tttcctcaag cgcgatcagc gcaaccatca tttttaccag tgacgcagga
    11461 taattttttt gatgcatccg gtcgtgcagt aaaatacgtc cggattcggc atcagccagt
    11521 atagctgcct tgtagtggga acggtatcgg tttacgctct ctgcagaaaa cgcctggttt
    11581 gtagttgcca gagcgaaaat aagtaatgct ccgtgaataa tggaataacg ttttaaaata
    11641 gttaaatttc gcaaggcctt atccttccaa tggaatttgg tgttgtgtat gaacaatgat
    11701 tgtctggcct ctatctcttc aaggatcgta cctttctttt tgtgttagtc agttctggtt
    11761 tctttaactt caaaataagc agaaatagct ttgacagttt cacatcaaac tatcattccc
    11821 agtaagcata tccagtagtt tgaaaacaac taaaatgata aattttactt gacatttttt
    11881 acgaattttc ttagcctgaa actatcttcg ttaggggtgg cggtttccga taaaggaacc
    11941 acctgagaaa gtcccttgga acctgatcca gatcatgctg gcgcaggaaa acgacacttc
    12001 tgtttggcgg tttctgcaac cgtctttcca ataccccctc tttgtcatcc ctgcgaaaat
    12061 tttattaagt gagtttccgg agtgtccgga gcctttcctt tcaaaaatta ttgttccgga
    12121 gattgttatg aaatctataa aattgtccgt tttacccatg ttgctcattg ttgtgggaat
    12181 tttcattaat ccaacatggg gagcggaatt aaccatttac acttacgact ctttcaacag
    12241 cgaatgggga ccgggtcctg tcgtttttaa acgttttgag aaacaatgtg cctgcaaact
    12301 aaaagttgtt tctccgggcg actccggaac tgtgctcaat cgtgtgattt tggaaaaagc
    12361 aaatccgaaa gccgatattc tgctgggctt gaataacagc gaactcgaaa aatcatttag
    12421 ctatgatttg tgggaaccgt accgttcgcc acttttggag caagttccag cagatttacg
    12481 tgttgacaaa aaacaccgtg tgaccccgtt tgattatggt ttcattgcct ttgtttacaa
    12541 tagtcaaaag ataaaaaatc ctccaaacag tctgcaggat ttacttgatc caaaatacag
    12601 aagaaaaatt gtgattgaaa atccgaaaac ttcttctccg ggcttgtcga tgctgcactg
    12661 gacgatagca gtatttggtg aagaagatta ccttgattac tggaagaagt tagagccaaa
    12721 tttactctct gtgaccaatg gctggtctgc ggcttatggt atgttcacca agggcgaggt
    12781 accgattgtg ttgagctacg taaccagtcc cgcatatcac ttggagtatg aaaaaactga
    12841 acgttacagg actgctgttt ttcaggatgg acattaccga caaatagaat ttgcgggaat
    12901 tctcaaaggg acaaaacgga tcaaacgggc acgtgagttt attgatttca tgctttccga
    12961 aaaatttcaa aacgcgatcc ctttgacaaa ctggatgttc ccggtgatcc agcaccagcc
    13021 gcttccggat tctttccgaa ttgcaccgca accaaaaaga gctccggaat tgaatccagc
    13081 gcgagtcaac cagcaaaact caaaatggtt gaaagaatgg tcccggacaa tgtcgcagta
    13141 acccatatcc aatcgtgaaa ccacatcctc ttaggcttct gcaaatatcc gcactgctct
    13201 tgcttggagc aggtttttat tggccccttt tgagctttct ttccggtaac aatcctttgc
    13261 atccggaaga aagttttttg cagtggaaat tttttaagga gtttttgagt gatccatgga
    13321 atttgcgggt cattagcttt tccctctatc aggctttttt aagcgcgcta ctttcgattc
    13381 ttgtcggact tcccggagca tggctgctaa catactataa ctttccgggg aagcgctggt
    13441 ttcgtctgct gacttacctg cccttcattc tgccttcgat tttggtggtt ttggcaatgg
    13501 tgctgtttta tggcaacaac ggctggatca atcgcggact gatgaccata ctcggaacgg
    13561 atgaacctca ggtacatttt ctctattcgt taacaggaat tttgattgca catgtttttt
    13621 ataattttcc gttggctatg aaaatcattg gagatcaatg ggagcgaata tcattgaaat
    13681 accatcatgc cgcaagttca cttggtgtcg ggaatatgcg gcgttttttc ggcattactt
    13741 tacccttgct gatgccttca atcggctctg cctttgtgct tattttttta ctttgtatga
    13801 atagttttgc aattattcta gtactcggag gtggtatccg ttacacgaca attgaggttt
    13861 tgatttatca actggcacgt attgaacttg atttctcagg tgcagcaagt ttagcttttt
    13921 tacagggagg attgagttta atcggcatgg caattatgct caaaggcagg gatcgcagag
    13981 ttgaccagga atctgcaagc aagagctggc ttccggaaaa actgaggaac cgatccatga
    14041 aagcatggtt tggtttgtgc tggattttgt tagtattgat ctttgcgctg ggacctttgt
    14101 ttgccatcct gatggattca ttccgcaagt ttgaacatgg tgaatggatt tacacctggt
    14161 attggtacag cgagcttttt acctggcgag aaaataatta ttttctttca tctctttgga
    14221 actctttgcg aatcggactc ggaagcgcac ttatttcttc catatctggt cttgggttgg
    14281 taagtataat tgcctaccga aaaggtgtaa agaggcgttt ttgggaaatg ctgactctct
    14341 tcccgattgc gctgtcaaca gtcgtttttg gtgtggcatg gttccatttt tatcaggggc
    14401 atctggcggg gactgttccg ttaatttttg tggtgatggc aatgcacgcc ctgctgacct
    14461 gtccatactg gatacgaatt gtacttccaa ctctggaaag cattccgctg cagtggcaca
    14521 cagaatcaaa aatgctgggc aagcaacctc ttgaatatgg tgtcagaatt ttgtggccct
    14581 ggctgagcaa aacttttctg atcgcctttt tcttcagttt ctcactgtct ctgggtgaac
    14641 taaattccac catgatgatt gccgatgaaa ccgtacgtac gcttccactt gaaatttatg
    14701 gagcgatttc aggatatcgc ttttcttatg catctgcagt agcagtcatt ttgcttatcc
    14761 tatcagtatc tacatttctt ctggtggagc ggagtattgg cttttttggc cggctaaaat
    14821 aaataaattt gggctttcaa ggaaaaaact ttaaattgcc gaaagaacag aatagcataa
    14881 gggaatcgca ggtctcggtt tagaaaccaa aaaatcgttc agtgaaggga tgtatggagg
    14941 ttggaggtat aagagaagaa acatgtactg cagaagaaac tgcttgcgaa aaaattaatg
    15001 cttctgttgt ggaacaggaa ttggagactg caatacttga atatgcaatc agtacattcc
    15061 ctttctcaag tgcctttgag aacgttcctg gttatcgcga agaagagctt ccggatttga
    15121 tagaaggtct tatttctgaa ggaatcgtca aggtcggttt ctactacgaa gaaatgcagg
    15181 aaaacctgac aatcttatat tttcttcgac tggatgaaga aaaacacgtt gttctgggac
    15241 ttgatccaaa cgaagtctgt gtcagtattt caaatgttga tggtattttt cgtttgaacg
    15301 atgaccaata catgaattta ctgaaaatcc cactctgctg aatcattctg attttgctaa
    15361 attcccgaaa aaattacagt tttaagttcc atgtctgaat tatcggatcc tccaacactt
    15421 cttaaaacag aagctattca actctctcta attggcatgt ccggcgcagg aaaatcgcac
    15481 tggtccaaga gaatggaaag cctgggattc caaatgtata gttgtgacga tctaattgct
    15541 gaacgtcttg gacagaaact tgaagagaaa gggaagtcca caataaatct tgctaagtgg
    15601 atggggcagc cattttcaga aggatatccg gaggcagaag ctttttatct tgaacttgaa
    15661 ggtgccgtta tctctcagat ctgcgatgag ttggaaaaca gcagtcaaaa agataagcaa
    15721 gttgttgttg acacaaccgg aagtctgatt tatctggaaa aaaaattact caaccgtctg
    15781 cgtaatttga ccttgactgt tcaactaaaa ctgccggaag aaaaacatga gcagcttttt
    15841 gaggcatacc tcttggatcc taaaccagtc atctggggag gggaatacct cccccgtgaa
    15901 ggagaaagtc cccaaaacgc cttggggaga tgctacaggg agttgctttc tttccgcaat
    15961 gaaaggtacg gtttgctggc agactgtgtg ctggattatt catttcacca ctgtgccaag
    16021 acaggagttg aagaactgct tgagttagtt acaaacaact acaagatgaa gccctgaatc
    16081 ttccaacaga ccgcttatct ctttttcaac attaagaaac aacaatgctc caaccatatc
    16141 tttgattgtg tttcagactg tctcaaaaag attttactac ctgcaaaaat acttagcctc
    16201 agatgcaatt ttttctgaag gaaatacttt aatcaactct tgccgattca ttatttttca
    16261 aagtcgataa ttattacttg aaattaattt ggcaacagtc tataattcaa ttttaatcta
    16321 ataataagga ggcactatgg aaatttttac tcttgatgga tggtatttat tattgcgttg
    16381 gatacatttg ctcgcaggca taacctggat tgggctcttg tattatttta attttgtgca
    16441 gggtgaatgg tttaaagaaa cagatgcgtc cgccaaaacc gcggcagtcc aaaaacttgt
    16501 tcctcgtgct ctttggtggt ttagatggtc cgccatgttc actttccttg caggtgcact
    16561 gatcttgatt tccaaaggta tgaagggatg ggaaatttat gcaacatcct ggggcgttgt
    16621 aattcttacc ggttccgctc ttggaacatt tatgttttta aatgtgtggt tgatcatctg
    16681 gccgaaacag caagttgtaa tcgcttctac aaatcaggta gcagaaggcg gagaggcttt
    16741 gcctgatgct gcaggatgtg cggcaaaagc agcattggca tccagaacaa atacattgtt
    16801 ttcaattccg atgcttttaa tgatgggtgc ggcaagtcat ttccccgttg gtgtgacaga
    16861 gagtacaagt ttctctggac tgttttgggt cctcgctata attatcgggg tgctagaaat
    16921 taatgcagta atcggtaaac ccggaccaat ggcctccgta aaaggcgtaa ttacttccgg
    16981 ttttgtcctg acagtagtac tctttggagt tataggatta ttggtctaag cactttagat
    17041 catgcttatt ttttaacttt gttttcgaac gcaatagttc ggagttagaa ataattaaag
    17101 ataagccact cccaccgctt tacatctccc catattttta aatatgggga gatgtaaact
    17161 gatttcattc caccggcaat ctcaatccca tttccagtaa tgccccgctt ttgttctata
    17221 ctcattttgt ttttattgct cacaagttgt gacctaaaca ttttcaaaga aactgataca
    17281 gatgtggaag tggtttctga gaaaaataat gagaccctga ctttaggcag tgatcctgaa
    17341 aaaggtaaga tcgtttatta tgccaactgc atttcctgcc ataattataa tccaaaaaaa
    17401 cctggaagta tcggcccaca aatatacagg tcttcaaaag aacttctctc aaaaaaaatt
    17461 aatttcggaa tatatccaaa aaattacaaa ccaaaaaggt caaccaaaat aatgccttta
    17521 cagcctcatt tagataagga gattgcaaat ttacatgcat ttttaaatgc tccttaatga
    17581 gcaagaacag agacgcagat aaagcttcta tatttaatag gaaaaatttt gcgaatttag
    17641 aaagataact tggtgaagcg ttgatggaaa caaataacgc aactttgaca tatatattca
    17701 tttccttcaa atcccccttt ttatggcatg agaattgcct ttttctactg atacgcgtga
    17761 gtaaccgtga gtaagcctgt gcttaatttt agtttaactg aagccctaga aaataagcaa
    17821 agatctccat aatgttgcca tgccggttta tcagctgatt cctgaaatac ccctgtttcc
    17881 accaatagaa gaggcggaag atgacggttt attagctgtt ggcggagatc tgactaaaga
    17941 gcgtttgctt ggtgcatacc ggagaggaat tttcccttgg tatgaagtag gtcaaccaat
    18001 attgtggtgg agccctgatc cgcgtttagt tttatttccg gatgaattaa aaatttcacg
    18061 cagtctgcgc aaagtcctgc gcaaggaaca gtttgaaatt cgtttcgata ctgcatttca
    18121 ggaagtaata aaatcttgtg ctgatgtcag gacaaagcag ggagaaggaa cttggataat
    18181 tccggaaatg cagcaagcct atactgagtt gcatcaggag ggttttgcac attctgttga
    18241 aagctggctt gatgggaaat tggtcggagg tctttatgga atttcaatgg gacaatgttt
    18301 ttttggtgaa tctatgttca gtacaatgaa cgattcctcc aaagtggctt tagttgcctt
    18361 ggcagatttt tctcaacgag taggtattaa actgattgat tgtcaaatga caacaccaca
    18421 tctgttgagt ttgggtgcga gagaaataaa acgggaggtt tttcttaaaa tgttgaaaaa
    18481 acatcttgaa accccctcta tcaagggtct gtggaataac ggtcctgttt caatgaaagt
    18541 aaacttgtta caaaattgaa atgccagaat caaaagggaa aaaagttttt cgaattcaac
    18601 tgacggtttc agttgttgtg cttttgttga ctctgagtgc cttcagcctg gttgcagctt
    18661 tttatttagg aatgacaacc ggcattagca tgcagcgtcc cccagaaggt tctgttgcag
    18721 acacagatat ttctgcagat aagcctttgt cagaggaaga actgaagttt tttggactca
    18781 gtgagcgaaa aaaagaccaa gattcgttcg acatggaaga gttacgtgac ttgaaaaaga
    18841 aaacaactga actaactaaa gctacaaaaa caccagattc agcctcaccg aaagtaccaa
    18901 aaacagaaac tccaacagtt aaagcacctg atagtcctgc ttctgaacaa aagactgtgg
    18961 ttaaaaaatc tgttcctgtt cccaaaactg tcgttcctaa aaaaccggat aatgttaaaa
    19021 ttcctgtagt caaatcaaag aaagatgcaa cttacactat ccaggttttt gcttcacgaa
    19081 agcatgcaaa tgctaaaaat ctgcttgaaa aacttaagca aaacggtttt aaagatgctt
    19141 ttatttttaa acatactgca ggaagtaaaa cactatatcg ggttcgagtt ggcaaaataa
    19201 atcgttcaga gactaacaat ttagccaaca aactcaaaaa acttaaattt attgattccg
    19261 tccaagttac acgcttctga agtgtgtcca aaattgataa actcgttaaa agtctaaata
    19321 tcacacttgt ttattaatat tcagcttaaa gctttcaatt acatacatct tgatatccag
    19381 aacaggactc catgagtaat tattggcgtt tttcacgctc agcttattat tctgttgttg
    19441 cagcgctgcc gttactggtg gcatacgaaa tactggttat tctgacacaa tcccgttatt
    19501 ggagtctcag gaatgccgca gatatgtgga tccggacttt tctaatggcc ttcgatcttc
    19561 aagcgcaaca cctgactttt gtgatgatcg gcatcttatt cgccttgatt ccgttagcaa
    19621 aatcttactc taccggagtt cgtcttaagg ttaattattt tgcactgatg ctcgcagagg
    19681 cttttgttta tagccttctt cttggaggcc tgctgcagtc aattttacgg gtgagtgggc
    19741 tgtcttccgg aggacctgga aatggtccgc ttcagaactt tgcactttcc ttgggagcag
    19801 gtttatttga agagatcatt tttcgagtac tattgctcaa cctgcttttt ttactcttga
    19861 aacctttctt gaaaagcaag gtcggtgtag ccgttgcttc tgttttagtt gcctcattcc
    19921 ttttctcaat tagtcactat attggaacta tggctgatca atggcagtgg tacagtttca
    19981 tgtttcgctg ggttgcagga ttattgttca ctgtccttta tttcatgcgc ggctttgcca
    20041 taacagccta tactcatgca ctatatgata tttgggttct tgtttgattt tgatttttca
    20101 gatttatttt gataaattta caatcaaatt ttttccagtt caatccaatt cgataattgc
    20161 caaaagccca tcaaaaaatt atgaaagtac cattgctcga tctgcgtcct cctttggagg
    20221 atttacgtga tgaaattgtt gaagcaatta ctcaagtcat tgattcaacg cgttacatta
    20281 tgggacctga aattgacagc ctggaaaaag aaattgctga atattgcggt accgcggacg
    20341 cagtaggagt gtcgtccgga actgatgccc ttttgctttc actgatggtt ttagatgttg
    20401 ggcctggaga tcttgtgttg actagtaatt tttctttctt tgctactgcc ggcgttgtcg
    20461 cccgtctgaa tgcgactccg gtatttgtag acattgatcc ggaaacatat aacattgatc
    20521 cggaatgtgt gagaatgact ttggcagaaa tggataagga aacccggaaa cgagtaaagg
    20581 ccattattcc ggttcatctc tacggccaat gtgcagacat gaaagcaata ttaaatattg
    20641 ctgctgaatt tgaaattcct gttattgaag atggtgcaca ggcaattggt gcagagtgcg
    20701 agattgatgg gaaaaagagg cctgcaggca gtttgggtga ttttggctgt ttttccttct
    20761 ttccttcaaa aaatttaggt ggtgttggag atggtgggat tgtcactgtc aatgaacctg
    20821 aactagcaga aaagttgcgt ctcaaacgag ttcatggagg cgagcgaaaa tattaccatc
    20881 gtgtgattgg aggaaacttt cgccttgacc ccattcaggc aaccgtaata cgtgttaaac
    20941 taccacacct gaaccactgg cacaaccagc gtcaggataa tgctgagtac tacaacaagt
    21001 tgtttgctga aacaagttta ggtgggagta taagtatacc tcaggtcatg cattccaaaa
    21061 ggctgcaaaa tcctcacatc tacaatcagt atgtaatcag agctgaacag cgtgatcaat
    21121 tgcaatcatt tttggcagaa aacgaaattt cagcagaggt atattaccca cttccttttc
    21181 acctgcaaga atgttttctg gatttaggcg gtaaagcagg agacttcccg gtttctgaga
    21241 cggcagcaga ggaggtactt gctttgcctg tttatcccgg attaagtcgg caaatgcagg
    21301 aagcggttgt ggagaaaatt gaagaattct atcaggctta accttctttt tccttgagtt
    21361 tttcctgccg ctccttgatt actttttcct gcacaaagga aggacattct gcatacttct
    21421 caaattccat tgtgtaattt gcttttcctg cagacatgga gcgaagagtt gttgcataac
    21481 caaacatttc cgctaaaggc acaccagaat taatcacggt gtcgtcacct tttacatctg
    21541 agccataaat cacacctcgc ctggaactca agtctcctat cactgaacct tgatactcac
    21601 ttggagtttc cacttcaact ttcatgactg gttccagcag taccggattt gcctttgcaa
    21661 cagcctggcg catggcgtaa cgtgatgcta attggtaagc taaatcactt gaatcaacat
    21721 cgtggtatct tccctcattt agtgtaacct taatattaac cattgggaat gcggccaggg
    21781 gacctttttc catcacatcc ttgaaacctt tttcacatgc accaatgtgt tctgacggga
    21841 ttgaaccacc aaaaattttg ttctcaaatt caaaaccttc ttcatgatcc aaaggcaacg
    21901 gttcaattga tcctgtgact cctgcaaatt gtccggaacc accagtttgt tttttatgca
    21961 aataatcgta atccgctgct gtcgtgattg cttctctata gttcacttgc ggagcaccaa
    22021 caataacatc cacttcaaat tcccgacgca ttctttcaat gtaaatatca aggtgcagtt
    22081 cacccattcc cgagataatc gtatctccgg attcttcatc agaggaaacg tgaaaagtag
    22141 gatcttctcg catgaaacgc ccaagcgctt tactcaattt catgtgctgt tcattatctt
    22201 taccttttac tgcaagagaa ataacggcat ccggaacaaa aatagattca caggaaacac
    22261 ggattccctc accacaaaaa gtatctccag aagcacaatc aactccaacc attgcaatta
    22321 tatctccggc ttctgcaaca tcaatgtttt cacggtcatt ggagtgcatt cttaccatac
    22381 ggccaacacg catcttgttg acggttctgg aattagtaac ctggtcacct tttgatagtt
    22441 tgccctggta aaccctggta tatgtcaact gtccaaattg ttcttctgtt agcttaaaag
    22501 ccattgctac agtcggttta tccgggtcgc aactcaaaag aacttcttct tgtgtcttaa
    22561 tgtcagttgc tttagttgaa gcagcttcga gtggcgatgg gaggtagctg atcacagcat
    22621 caagaagtga ttgaacacct ttatttttga aagcacttcc catataaacc gggcatagct
    22681 ccaaagactg tacacctttt tttactgcag catggatgct ttcttctgca atttcttttt
    22741 cttccagtaa atcctccatc atttgatcat caaacatgga aacggcttcc agcatttctg
    22801 cccggtaact ttcggcatct gatttcatct catccggaat ttcctcaaac ctcagatcat
    22861 caccatgctc accgtcaaaa taaatagctt tcattgtgat caggtcaacc actcctttgt
    22921 gattttcctc aagtccgatt ggcagttgca tggctactgc gttcaaatca agaatgtctc
    22981 gaataccctt aataccatta tggggatttg cacccattcg atccaattta ttaataaatg
    23041 caagtcgcgg aacccgataa cgtttcatct gacgatctac tgtgatcgac tgagattgaa
    23101 taccggccac accacaaagg atcataatcg ctccatcaag aacacgtagt gatcgctcaa
    23161 cttcaactgt aaaatcaaca tgtccgggag tatcaataat gttaattttt gtatcattcc
    23221 attccacagt tgttgcagca gaagtaatcg tgataccttt ttctttttcc agttccatgt
    23281 ggtccatggt tgctccggca cctcctccac gaacctcttc aattttgtga attttgccgg
    23341 cgtagaataa aacacgctct gtgagagttg tttttccgct gtcgatatga gcggaaatac
    23401 cgatgtttct tgtattttca agaacttttt gagatgcagg attcattagc taatctgttt
    23461 ttttatggga tttgtataga aataataaaa aaaatataat cttatataat agatctataa
    23521 taagatttta tcaagcacta ttcttaaact atatacaagg agaatttatt agcctgtcag
    23581 cgaatgttat gtaaaaatga gttttgcttc actaaaattg ctgacgtaac cgatacaagc
    23641 agagggaaac actccgacat tatgcaggtc ttttagaatt ggggtagttt catcttccgg
    23701 aactgcgacc aaaaggccgc cgctggtttg tggatccagc attaattctt gcagtatcgg
    23761 tgcacttcct tcaggaaatt tccagtattt ttcaaccaaa ctgcgattgg tttcatttac
    23821 accggtactt acaccacgct cgtacatttc cctggcttca ctcaaaatag ggatatcatc
    23881 aagtgtgatg ttcaatgtta agtcagaacc gggcaccatt tccatgcaat gaccagcaaa
    23941 accgaatcca gtgatatcag ttgctgcgtg tatctcgtag ttcgccaata cttcagccgc
    24001 agctttgttt agttcaatta aggagtccag acaatcctga agcgcttctt ttgaaactaa
    24061 attttttaga ttagcattta ataaaacgcc actgccaatt gccttcgtca aaatcaactg
    24121 atctccaggc tgagcaccga tatttctcca aattttttca ggatgcacca accctgttac
    24181 agtcagtcca aattttggtt catcgtcatc agtggtatgt cctcctaata gaactgctcc
    24241 tgcttctgtg attttttgca gagcacctgc aacaatcctt tccagcacct catcttcaag
    24301 ttttccggaa ggaaagccta caagatttag acaggacaaa ggtttgccgc ccatagcata
    24361 aacatcactt agagagttgg ctgcggcaat ttgtccaaat aaataaggat catcaaccgg
    24421 tggtgtgata aaatcagctg tgaaaacaag agcctgttca tcattcaaaa gatacactcc
    24481 tgcatcatca ctggtgtcaa aaccaacgag cacattaggg tcattatttt gaggtaaaga
    24541 agccagcatt tttttcaatc cgaccggatt gaattttgat gctcaaccac aggtttttgc
    24601 caaacttgtg agactgggct tttgaaaaat ttgtttcaat ttcatagaat ttattttgct
    24661 gtacatgcat tgcttgcaga gaagcaagcc tgatcaagag tttttactct gcattcaaga
    24721 tgctccatct gcaaaataca gaaatttaag gatttcagta aatccgtaaa taaacaagac
    24781 ttatacatca aaacaggctc tgatggaatc cggaatgacc tgagcctgta ggcgttttgg
    24841 gtttgaagga tgtggtttgg cccacaccct tacttctgta ccctttacgc aaagttggcc
    24901 gttgttatga atttcgtggg atgcaatcat tgtttttgcc cgccactcac tgatccaact
    24961 ggtaacttca atcacatcgc catatttaga aggacaaata aattcagcat gagcatctac
    25021 gatcggtagg ccgacaacgc catattgttt cattaaatcc cccatattcg caccggcttt
    25081 gtcaaacaaa agatgggcac tctgatcaaa ccaaatgaaa taatttggat aaaacacaat
    25141 ttttgcggga tcacaatgtc cccagctaat ttgaattgtg tgtgtatatt ttagcatgaa
    25201 tttccgctca ttattagatt atctttagga tgaatcagca cctgtgaatc agcagactta
    25261 gcagggagaa ttagtaaatt tctttgcctt caaaaaagtg cgattgtcaa ctgttgaaat
    25321 agtaaagcat aagcagaaaa gggaaagaat gcaacgatct atattgacaa acattcatca
    25381 cagaagcaga atgattgaac cagttcccgg aaatacaggg agagagaatc aaatataatt
    25441 attactaatt taaagggtga gaaatgggaa ccgttttcaa ggtgcaggaa gccccgactg
    25501 tgaatgttac aggcacggga gatagttttc cggtccatcg aatttattgt gtgggtcaaa
    25561 attatgcgaa tcacgttcgg gagatgggag caaatccgga acgagaagcg ccttttttct
    25621 tcagcaaacc tgcagatgca gtttgtccgg aaggtactca agtgccgtat cctatggcaa
    25681 ctgctaattt gcaccatgaa gtagaactga tcgtggctgt tgggaaaaat ggtacaaaca
    25741 ttccagcatc agaggcactg gattatgtct ttgcttattc agtaggactg gatttgacac
    25801 gtcgtgacct gcagaagtaa gcaaagaaga aaggcaggcc tgggctacag ccaaaggttt
    25861 tgaccgatct gctccttgtt ctgatttgca taaggtggag gaagttggtc acatagagcg
    25921 aggtaaaatt actctatctg taaacggaga gatacgtcag cagggagata ttccggatat
    25981 gatttggtct gttgcagaag ttatttcagg cctttcaagt ttttttgaac tgtgtccagg
    26041 agacttaatt tttaccggca cacccgccgg agttggcccc ctggaatgtg gtgatatact
    26101 ggaaggtgag gtggaaaatt tggcaaaatt gaacattaca atcgtgtgaa aagaaaattg
    26161 attgaatttt tactaaagtt tcatgaaaat ttgccgagat taatagtagt tcatttgctc
    26221 aggaatttta tgtaatagca ttactgattt gtatgccttt tgagcaacac ttttttcaac
    26281 atagtacaaa gatgaaaatg atcaatattt cacaaaaaac atcttggtta gttattttct
    26341 ctggaattat attttttggt tccggatgct caaaattggt cggttttacc aaagaaaata
    26401 ctactcctac ttcttgtgct gaccgtgcaa ttgaacgggc ctttaacctg cacgagggtg
    26461 ccaagaccgg tttggctttg tattttgagg aacgaagtga caatcaactt tatctggctt
    26521 tctatgcagc aacagattca gtttatgaat ccaggagggt gaaaaagtgc tgggatagaa
    26581 gggtttctca ttacaatgca atgcagaatt tatgggaaat gaacacggca ttagccaggg
    26641 tgattcgacg caatatgccg gatgacgacc gtggtgaact gatctcagtt ttccgcgaac
    26701 aatacgattg ggtgatgcct aattttcgct gaatttccta acttgccttt ttgctttcat
    26761 cagtctacct tatatcttct gttattcccc atcgactccg gtcacttatt ttttcagtaa
    26821 ttttaaattc ttaaaaagat atttcaaatg agcgaatctt accctatgac cattcagggt
    26881 tacgaaaatt taaaaaacga actcaaagaa ttgaccagtt tagatcggcc cgctgtggtg
    26941 aatgctattt ctactgcaag agaacacggc gatctaaagg aaaatgctga ataccatgct
    27001 gccaaagaac aacagggctt tattgaagga agaattcagg aattgaatgc aaagctggct
    27061 ttggcatatg tgattgatat tactaaactt tctggtgaaa agatagtgtt tggagcaact
    27121 gttacttttg tggacgttga tacagacaaa gaaaaaatct atcagctggt aggtgatgac
    27181 gaatcagatc ttgaacaaaa taaactttct atttcctcac ctattgcccg tgctttgatc
    27241 ggaaagtctg ttggtgatac tctcaccatt ccaataccca aagggaaaat tgaggtcgaa
    27301 atattggaaa tcgaatttat tgtttgattt caatttttgt ggtccaatat tgaattcggc
    27361 ccgtctttca gaatcataat acttcggaac acttctttca aatcttcaag tgtctgaatt
    27421 tcttcaaagg gaagatttag cgactttgcg gtttcctgat aaatccaagt tggattccgg
    27481 tgtttttccc gtaacatttg tatagaatga gctccttttg attttgatag tttgtctccg
    27541 gcatcatctg tttgtaacac atggtgtaca aagcgcgttt caggaaagat agaatcgttt
    27601 aagcatttcg caagataaag ttgtgcacca gttgaagacc tcaaatcata tcctctgact
    27661 acaagattta ctccaagttc cagatcatcc atgatggacg caagctgata agctggaagt
    27721 ccatctctcc gttttattac aaaatcagcc atttccctgg aaacgtcgat tttctctatt
    27781 ttgtttgtaa atgtttttaa acagacaaga gtttgcacag gaacaaggat ccgccaagtc
    27841 tgattacctt tcagtaaatt gagttttttg ttacggcaag ttcccggata gtttccgttt
    27901 gaagatattt gtctgatttc tgaacgggag cattcacagg caaacaggtg tccggatttg
    27961 cgaagaagtt caagtgattc atggtaaagc tccatgcgta atcgttgtga gaattttgag
    28021 aataactcgt caggtccgga aggtccggaa tcgtatttga tgccgagcca ttccaactgc
    28081 acaaaaatat cttccacagc agaacgttcc acccgggggt cgtcaaggtc atcaattcta
    28141 agatgcaaat gtccatcctg aaaatctacc agcaaagcag tcagcaaaaa attgaaagca
    28201 tttccaagat ggagatagcc gctaggagtc ggggctaatc gagagatgac aggtgagttc
    28261 attaatgttt tttgtagcgt tagcaaataa tttactggaa tttttacact actctgcttg
    28321 tttttagagt gcaagtaatt atcatggact aaccactatt ctagctaaat caatgtatat
    28381 gttcgggatt tgaataccaa acaggtcttg ccacgctgaa attcagtgaa cagatttagt
    28441 gctggttatt tctgaagaac agcattcgaa aagaggcctg ggaagcagga agttgaggag
    28501 tttcagaatt gtacattgat gaagtttttg catattaagc tcgatttgtt ttggtgcctg
    28561 tactaagata acacatcagg aataagcata tgtggaaaat gaatcaaacc gattatttca
    28621 gtctatctgc aaaagttttt ctgtaaaagc agagcatttt atgtttgttt taaacaacac
    28681 aaagttcagg tttaaataat aatcaacgaa agatttaaat gtacgaaaca tcagatatca
    28741 ggaaaggtct ccgagttgaa ctggatggtg gtccatttgt ggtagtggac tttcagtttg
    28801 taaaacctgg aaaagggact gcatttacac ggacaaaaat ccgaaacatg attaacggtg
    28861 ccgtgataga ccgtacattt aagtcaggtg aaaaactaaa accggctgat acagaagacc
    28921 gtgaaatgca gttcttatat agagatggag aatttcattt catggataac agtaactacg
    28981 aacaagtaag tttggaaagc agcgttgtcg gtgatgcttc gaattatttg acagaaaaca
    29041 tgatgattga agtgagtttt tacaaggggc gttctattgg cttaactttg ccgaattttg
    29101 tagttttgga agttacagaa actgcacccg gtgaaaaagg aaacacagtc acaggtgcct
    29161 caaaaccagc tatggtttcc accggataca cagttaatgt accattattt gttaatgaag
    29221 gtgatcagct tagaattgac actcgaacag gtgaatatgt tgaaagagtc aaagcctaga
    29281 acaacttcaa attcgctctc aatactccag ccagaactca aataaagttt ttctttaggt
    29341 atccgatcat gtccggatct tcaaaaatat tcggcaattc tctgttatcc gggaactata
    29401 atggccgagt catagaagtc tctttcgaga ctgaacaatc tcaaggatca tctcccgagt
    29461 atctatacag tatcctggtt ttagttgaag atcagttgtt gccgttgtcg tttcgagaga
    29521 aagaaaaaat taaaaatggt gcattaattt cttttggagt gactgaagaa agtgagatcg
    29581 aaatagtaaa aataaaaaat catgctttta attttgatgc agagagtgac ttgcttcgct
    29641 ggagacgtcc ggcaaataat ccttcaagaa tggagttgct ccggactcgt cagcgaatct
    29701 tacgcggtat cagggagtgg tttgaagaac aaaattttct agaaacagag actcctattc
    29761 tggttcgtgc accaagtccg gaagcacaat ttttccccga aaaaaccagc tccggttatt
    29821 taattacctc tccggaattt cagatgaaac gtttgttgtc aggtggtttt gagagaatat
    29881 tccaatttgc gcgttgtttt cgcaaaaacg aatccggacc tttgcataat cccgaattta
    29941 caatgctcga gtggtatcgt acaaacgaac cactggcaaa gctgatgtcg gatattgagc
    30001 aaatggttaa gcatcttact gaaacggttt cagcacaaag ctttcctgaa caagttccat
    30061 taccaccatg gcctcgtgta acagtgagtg aattatttaa aaaacatttg gacattattt
    30121 tggatggttc tgaaaatgct gatcgcttaa gagaaaaagc aaaacttgca ggattttatg
    30181 aatcaaaagc tgagtttaat cctgcatcaa aactcacaga atctcttgat tatgagcaaa
    30241 tgtttttcaa actttggaat cattttgaag cagatttagg aatagtatct ccaatttttg
    30301 tatatagttg gccacttcca ctggccagtc ttgcccgtca ttgtcctgag cacccaggat
    30361 ttgccgatcg agttgaacta tatgccgacg gtatggaatt ggccaatgga tttggagaat
    30421 tgactgatgc atccgaacaa cgtaggcgat ttgaacaaga cttggaaaac aggaaagcag
    30481 agggccgttc tggagtacca cttgatgaaa atttactaaa aagccttgag cagggattgc
    30541 ccgagagttc aggaatggcc ttgggagttg accgtttgat tatgtggctt tgcggtgtac
    30601 aaaatattcg agatgtgatc tgtttttctg aggatgaaat ttaagcagga tttcggttgt
    30661 actaatctga aattaataat ccataatcat agataacaaa atggaacgtt caatcattca
    30721 cattattctt cattttttgg taccgttcat agtagctaaa actgcttgga gagaactatg
    30781 gctatggccg tttattataa tggctctgac tttcgtaata gattttgatc actttctagc
    30841 tgaacctgtt tttgatccaa accgttgcag tctaggtttt catcctctcc atagttggcc
    30901 tgccattgga atttatcttg tgtcactttt ttctcctcgt ttacgaatta ttgctattgg
    30961 attgctcata cacttggtat tagatggtac agactgcttt tggacagact gacttatcct
    31021 gtgatttctg catcaaataa caccttagaa gaaaaaaagt aaaaaaatgc attttttaaa
    31081 gaaaaagctt gacaaactac tagcactctt gtaaagtgag tgctaacaaa atgtaaatcg
    31141 ttacttcaaa tggttcagca aggtgcgaaa agggtcaaaa gttgacgtaa aattatagta
    31201 ataaacggac ctgctgagtt aatataggat aaaactttaa ttattgagga aaaaatggct
    31261 aaaaaagcaa aaaatatttc acatgaagaa gtaagtgaag cgatggcccg ctttcttaaa
    31321 aaaggtggtg tgatcgaaaa gatagaatac aatgataatg gatttttact gaaaaatgat
    31381 cttgctttgg atgactctta cactgaaaca tcggacagca tcatgagcca actgcacata
    31441 gactttgaaa cggaaacgcg aatctaaact gcttctttat aaagatctct aaaatgcagg
    31501 cggatataaa atatccgcct gcatcccccc gtaaatttca cctcttccgt tatcaatctc
    31561 aggattcttt acaaaagccc actacttagg tatagtttgt aagcatgtat tgaaatacat
    31621 tctaaaatta taatacgccg tgtattagaa atttttcagg gcttgggtgc taaagttttt
    31681 attgaattca atattttccc gacattggca cgaactgtag gaacgtcaag attattatcg
    31741 tcgaaatatg gatgatcgtc cgcaactcca atgcgtttca taattttttt gatgataaca
    31801 cgctcctcgt cttcaaatac accgtcagat gctgccacta aagccattaa ttcaaccagt
    31861 gtgtgctttg tttctactga ctgaatgtgt tttaacatct cttccagaga agtgtttgat
    31921 ttcatttttt tctgctcttc ctgtgtgatt cctgcagctt tgaaaacagc aatcaggact
    31981 ttcttttcag ctacgtggac atccctttca gcatgtgcta aaaaacttac gactgaaaaa
    32041 atattaacta gctcatcacg actgattgac atgaaatacc tttcttaaaa aaaacaccgt
    32101 agtatacagc tgttgtaatt tgcaacatta taatttcatt cttaataatg tataacgaaa
    32161 ttcggaaaga acgtaaagaa aaattattat tctcattgat tcaggaagga aaaaatgggc
    32221 cgtgacaccc gttatcatcc tgaatggagc acagtttcac gttatgtaag ggaactgttt
    32281 aattattatt gttccagatg cggaaaagat tgcagaaata caaaaaatgc agaaatggtt
    32341 ttgcaagtcc atcatattga tgaaaatcct ggaaacaatg acctggaaaa cttgatcccc
    32401 ctttgtgcga gctgtcatct taaaattgaa cgggaagcca gattacacgc accaaaccat
    32461 gaaaaacaac tggaattgtt tgaaaaccaa tcgtacatga gtcagatgaa aatgatgcgg
    32521 cagagtgcat tagtaaaaca tggtgctgtg aacaaagcag acgtttcaca gatggatgcc
    32581 gaaacttacg aaataagggc tctggattgg gaaattaacg aaccgtctta aattccaagt
    32641 aatcaattgg tgcccaactc ctacacaaac actgaaccaa atcagcgtct tcttcttccg
    32701 aaacctccaa aaccaaaccc tcgcatgcgt cggcttcctc ctccaaatag cgatcttctc
    32761 cgtgttgagg aacgtctgcg ttgtgtggca acaggttttc tttgtgtccg gctgttgccg
    32821 aaacctcctc cggaagcttt acgattagca gagcgaaact tagtactttt tatgttattc
    32881 agtgattgtt ttgaagtaga acggaacttg ttagacgaaa cacttcttcc actgccagtt
    32941 tttgcagcag actttgatgg agaagctttt tttagtgaag atgtggcagc accacgacgg
    33001 ttttgaacag aactaactgc cattggacca ccgccgtagc cgtaaccata acctcctccc
    33061 cacatgaaag gtggacgaaa gagcatcgac atcatcattg aagtcataaa caaattagtc
    33121 atgctgtagc cggtgccgta atatggagga taaggtgccc cggctgattg atacatctgc
    33181 gggttcggtg cggattgcat ggtcatttga tcacctgaac cgcggaccac tgaggctgtc
    33241 ataatttcaa tagacttgtt ttctgcaatt tctctccaag ctccattatc tgacacagaa
    33301 ggaagaacat taactttatc aggatccgga accaaaatcg aaaatacaag gaaatcagaa
    33361 tcagctgttt ctgtttgagg tacaatcaaa acaggatctg caattccgtc ttcattaagg
    33421 tcaatcgtgc ttacatcacg aattttattg gatattttac taaccagaat aggaagaaaa
    33481 ctgtccttgc taacattctc ttgcgtcgtt tctcttattg cctctgaagt gatagccgga
    33541 attagttttg ggtctagatg ttctgtaaga ttaattccca gtttttcaag ggcttcagga
    33601 tttagttgat cactgactcc tatcggaaca ttctgagtcg taaaattgaa tcctctagat
    33661 tctcgattat tgtcttctcc aaaaaaaacc caataaaatc ctaaaatcaa aattgcgatc
    33721 aggataccct gggttgaaaa taatttttcc atcccgtctg acggttcatt atcttttttt
    33781 cccattgctc gtttcttttg cttaagttgt tagtctaaat tgaattttat taatgtctag
    33841 agactttact gcactttagt gaaggcgcaa gttaaaacga tgttaagttt caatcaagca
    33901 gtattggagg ctatcaaatt ttttccaaca gccatatcat gagtacttat tttgagataa
    33961 caatagctga cccactgtgc aaaaaaagtg ttgcgatgtg aacgccagcg gttgatggga
    34021 ttgttaaaat caaaatttac gggcgctggt acctctgggt ttccgtgatc tcgtttagct
    34081 tcttcagcaa gtcgggttgc attgtattca ggatgcccgg tatgtgcgat aaaacggtca
    34141 tcggtagtgg aaaaaatcga ataaccagct tcgggcccgt aggccagcaa ttttaagcgg
    34201 ccggattctg aagcttcttc cattgcttca tcaggcatac ctgcatgacg gctttgtgga
    34261 cagaaaaaca catcatccaa ctcaccaatg attggatgat cgggagccag atttttcagc
    34321 tcaaatactc caaaaagttt gtgatcatag ttaagttttt tcacaccctc caaataagcc
    34381 atcacaaatc ccgcccagca caaaccaagt gtactgggaa tatttttacg tgcatcactt
    34441 aaaattgttt tgatttcttc ccaataataa acatcttcat aatcaatcgt ttccacagga
    34501 gcaccagtca aaatcaaccc atctaaaggt tggtcacgca ttgcttcttc ataagttacg
    34561 taaatatcat caacatgctt tggctcccag gatttgtaat tatgtgattc caaacgaatc
    34621 caaattggct ctaactgtaa tacagaaagt cctaatggat ggagaatatt aaattcatat
    34681 ttttctccaa gcggcattat attgaggatc ccaatcctta gtgggcgaat atcctgtcgc
    34741 tttgcctgag atggtggaat ccagcggatg ccatttcttt ccaaaattgg cactgcgtga
    34801 taatcaggac gaacaatgag tgtcatttgc ttcttaatct aaatttgcag gtgtccggaa
    34861 ggacaactgt gtgttcaact aacagttcta ctcaaatgaa aaagacaggc aaagtctctt
    34921 cactagcaga aaacaagaac caacaaagtc attcttgcaa taggttaatc agatagacgt
    34981 gaatcaatca ggtgggtcac caaaagagca ggaccggcac tttactcgaa tcgagtcgta
    35041 tcgactccct tccgaaactt tccggagggc tggcattagc accttgatgc tgaaacagga
    35101 tgctcgacac ttcaaagggc cagatccctc agtgtcatct ggatacaaaa tttaatagat
    35161 tctatcacaa tcaaagttaa agtcaaactt tttcaatcac ttaataagaa tatagtgatt
    35221 tcaaatttca gaggggctca tcagagactc tttgagggac tcctcaaagt ttgactttac
    35281 taatttttca tgatcctcag gagtaatgta aaatgtatca atcacttgat gtccaatggt
    35341 tgtaataact gcctgctgaa ttggtactcc aacctgatca aatgcactga ccattttgta
    35401 ataaaccaag ggagcatcat cggcagtaat ttttatgcga agatagtttt tgtcacgtct
    35461 cgaaaaatct gttttttccc tgaactctcc attccaaaca gggaaatccc ccaaagcctc
    35521 acttttcttt tctttgacca tgtacccttt tttgtcatct ttgaggtact ccagttgaag
    35581 ctttgtgttg taaagaaaag tttgtgtctt tattattagt gcagggaggg tattactttt
    35641 gatggaagtt tccaattccc ggggtaaaat cacattctgc tcttctcttt ttgaaacctt
    35701 gagataatag caaaaggcaa ctttaccact cgcaaattga atttgattta tctttgccga
    35761 ttcaatattg aggtgcttca aatttaaagt ataagcaatt ctactgtgaa tttggagctg
    35821 atcatgtaca taaatcaaaa gtccgttaag acgtcctcgt gcattggtga tcacgtttac
    35881 gatagccggc cgctcaagcc tattgaaaat tgataaattt tctgccagca tgtctggagt
    35941 agaacttagg agaaaacgat ttggaaatgc cgcaaaaaac cagttaatca tgggactgta
    36001 aagttcagta agtgtcacca gacttttttt gctgatggat tgcctgatgg tgcgaataaa
    36061 tttatctgta gtttgatcag taccctgttt atcaagtgaa cccaacttaa ggtcccgcca
    36121 cagaacttgt aaatcgtctt cggattttcc aagtaattta cgttcctctt tgttgatttt
    36181 tttgagaggt gtcagcaata cagattctat attttcccgg agacttctgt ttatcaaaag
    36241 atcaatacgc gtatctgatt ctaaatcatt ctttttaata tccaggtatg tttgtatgaa
    36301 atccattgaa tcttcctgca acttagaaga tcgcatttcc gcaaacacac gatatgtttc
    36361 ttcaaaaaat gttctcaatc cccgtgtgga gtgagcattg ctttcactaa cagatatgta
    36421 atcagaaata ttgcatagga agagcaaaat gacattgatc aggttgcggt ccgcaatttc
    36481 aaaaaaactt tgcagagcct gatcgaaata tgcagaagtt ctgctcaatt gaacaactgt
    36541 ggtgtggtgt acaatgagca gagatacaag agtgaataat tcctggtcat catagccaat
    36601 acgttccagg gcattgacag caatagatgt cccggatact tcatgatctg tttgtggatc
    36661 aatttttccc acatcgtgaa atagaatgcc ccatttgaga gctaaaatat gttttggttc
    36721 cagatattga tataattcct gataatcttt ttttagccga tccagttctt tttgcgtacg
    36781 atcaagtgca ttgagggtat gtacgcaaac gggatgttgg tggtaagcaa gattcctgag
    36841 caagaaacgc attttgtttg tttccggaat gaaacaacct attaaagtac gaggttgact
    36901 ttcagcattg atcggttcgc aaatttctaa cattatccgc caggcatttg ctgtaaacgg
    36961 tgtcaacatc agtttcgtga aactttttgc aatctgtgtc cggtttgaag aaatcacgtc
    37021 cggcgttata atgcgaatca agtcggccat ctcatccttg agttcaaaag ataaatcgta
    37081 ttctgattga cccacatatt caaaaagttc cagcaacgct gttggatcgt tgaaaatcaa
    37141 agatattgga acttgcggca aaccttccag agcatttacc tcaacaatta gacgcttccc
    37201 cagatgaacg acaacctgaa aatttgagaa agtccgcact attgtacggt ccagcaggcg
    37261 ttgtaaaata gcttgtgaga gatcttgaat gtgattcacc atttgcagac gatagcggtc
    37321 aaaatcgtca atactctgga agcgtttttt cttgagcagg taaagacgtt cggtagcatc
    37381 attgatgatg ttactttgga tatctttttc ggatagtcca ctttcccttg cctcatcgtc
    37441 aaagtgatac tccttggcgc tcgcggaaaa gttgcgtaag tcgtaaagga attcgagggc
    37501 ctgacaaagt ttgtaaccct gacgaggtga aataatacgt ttttgtatca gaagtgccag
    37561 catttctgca gtttgacttg gaaaaattcc gaaagttgca gcagaaagcc aaagaggaat
    37621 ttgcagggag cgcaaaccac ctcgatcttc cttgagattc atctgactca gatcaggaac
    37681 tgtacggtca ttataatctc taatttggct caggacaaag gtttcatatg gcaagatctg
    37741 aaaaaatctt cgtttcaaag catttgctaa atctttgctg ccaattaaaa ctctgctttc
    37801 caaaatggat ggaattgtat ggataacact gggatctttg aaacgtgata agtcttcatt
    37861 taattcaaaa tattgatgtg ccgtttcaat acctgcttca cgcagtaaat gctcaatatg
    37921 aatgatgaac tgtcggcaaa tttctgactc accagcactt agttgttgtg tgtcgagaca
    37981 atatcccaaa tctcgatcgg aagcaaaact cagttcagcc ctgccataac cacctctggc
    38041 aaagacaacc aaatgattaa gtaataaatt cagcttgatc tcagatttct gtacctgcgg
    38101 taacaactcc tccaactctt tcagtcgttc gctgaggttt tcaatttcat gtatcaactc
    38161 agcttcaata ctttcataat atttcagcat ctgcctttga tccggctctt ctggatttga
    38221 ttcaactttc tctatttgtg tccggctaag actgagtttt ttcttttttt tcggtagaga
    38281 tcgttttttg aatagaagct cttttttgaa atcttccttt atcctttcct tcaggatttg
    38341 aagatcggca aacccatatt caaaagcagt atggataata ctgtccagta ggtgagtatt
    38401 ttttgaaagg gtatcaaacg aattgtttgt acagaggctg ctttcaacaa tttttttgta
    38461 ctgctcacga taatattcct caattactcc cgcaaacact ttttcacggg cagtaagata
    38521 ttccatagat tgacaggagt caatagccaa aggccgccaa acttcttcca gttgtttaag
    38581 caaggcttcc ttgtgaactt caagttcttc cggattctcg gtgaagatga aaggagaaag
    38641 aaagggataa ttctcaattg gagaaaaatt tgtttccatg aaattaaaac aggtaactga
    38701 taactgttaa agtgttttta aactgcacga tttaaataat cattaggcat caatgatggt
    38761 ttaagtttac atctaaccca gttttcaaaa tatcaatctg tagctcccag cataattttc
    38821 cgcaaaatac tttcaaaaaa gatctacctg agattgagga ggggctgaaa tttccaaatg
    38881 ttgataagct cgctcagtcg ccactctgcc tcgcggagta cgctgcaaaa accccttaag
    38941 caacaaaaaa ggttcataaa cgtcttcaat cgtgtctttt tcgtctgaga ctgccgcaga
    39001 aagagtgcta agacctactg gaccacctcc aaatttttta atgatagttt ccaaaatatg
    39061 acgatccatt tgatcaagac cctccaaatc gattctcatt ctttccagac ttctttcaac
    39121 caatggcaaa gtgatcacac cggacgtctc catgtctgca aaatcccggc aacgcttcag
    39181 taagcggttt gcaattctgg gagtacctct agctctgcgt gccagttcca aagaagcttc
    39241 ttcaacaatt tcaattccaa gtaatgatgc tgcgcgcaaa atgatagttt tcagatcttc
    39301 cgcatcataa aaattcagcc ggaactgaat tccaaaacgc tccctcaaag gagaagaaag
    39361 cagaccggca cgggttgtag caccaactag agtaaagtgc ggcaaatcaa tttttactgt
    39421 gcgtgctcca ggtccttccc caataatgat gtcgagagta aaatcttcca tcgcaccgta
    39481 aagcacttct tcaactaccc tgttcaatcg atgtatttca tcaataaaca aaatactgcc
    39541 tggatccagg ttagttaaaa tagcagctaa atcaccttga cgctcaatga caggcccgga
    39601 agtagagcgt agttgcacct ccatttcacg cgcaaagata tttgctagag tagtttttcc
    39661 caatcctggt ggaccactca aaaggacgtg atccagagca tgtccgcgtt ttattgcagc
    39721 accaataaaa acatgaagat tttccttaat ttcattctgg ccaatataat cctgtaaacg
    39781 ttgcggacgc aatgattgat caaactgttc tcccagtcgt gattctggag caaccaatcg
    39841 atcttcagag ttttccttag acacggaagt ctcagtgtcc acagtttgat cctgcatggg
    39901 tgtttattat gaagtagaaa gtttttccag gtgttcctgg gaagcgatgt ccggactgat
    39961 acgccaggta tacaaaggag aaaaaacata caaaatgagg tatccaagtg cagcatgacc
    40021 gaaatatgga gtaaagacga agccgatgac aagcactata gtaaaaatta aaaattttcg
    40081 acttcggctc atcaaacgac cgatgtgcat atagcgaatt ggaaaactta tcatcaggat
    40141 tgccgcaatt atcatcagaa agaaactaaa ttttgaccaa aacactagat caggagtatt
    40201 tgattcaagt gcgtttccaa tcattatgaa aggtgctgca actagcaggg ctgcacctgg
    40261 aacaggtatt cccttgaaaa atcccggaat gctgttttgg tccaaaatga agaaaactaa
    40321 tcttgtaata cctagaacga catacagaat ggcaatccat ccataaggga gtgactggat
    40381 agattcatct gcaacccgcc ccatcaacat ataaaatatt actgctggtg caatacagaa
    40441 actgacggta tctgaaacgt cgtcgaggac tcctcctaaa gtaatattat attttttggg
    40501 ttttgctgat ggtagcggag tagtgagtcc caattttctg gcgactgcac catctatttt
    40561 atcaaaaaat cccgcaccaa gtaaaatcag gtaggcttcc tggacccgtc ctcgataggc
    40621 aaataaaatt gctaaaactc ccatgagcgc attcattacg gtcaggacat ttggaaagac
    40681 ggctaatata cggcggcgta aaacctcatc gttcaaacaa agatcgtcga ggcaagcagt
    40741 accgtatttt cgacaataac cggcaataga accaaaattg atgataagga acagaatttc
    40801 caaaaagaaa agttgctgaa ttggaattgc tcccagccag ctgatccagg aataaagtga
    40861 agaatcagga ccaccaggga cataaaatgc gtgaatatag agcacaactc caaccggtgc
    40921 ggcaaccatt gtccggagtc gagtaacttc cttcatttct tcttcctcac ctttccggag
    40981 tgaaaaatta cgcatgaagt gtgcaaaatt atcccgcatt aagacagtca cgcacaggac
    41041 aaatacaaag acgacatgca gcatttccaa tcgaaaatcg gcactctccg gcaaatactg
    41101 ataacgccac atcattccca ctgcaaccat cggaaacacg atagcataca ccactttgtc
    41161 catgatcctg tctgctaaat gggccagttt tgcctgcggt cgaaatcggg ctgcaaacca
    41221 gccgtcaatt atatcaagaa tcattgcggc aagcagaaaa atcactccgc tgtagtaaac
    41281 gagggggtta tggtcaagca taacgcctat ggcgcagatc attccagcaa acaccatcgg
    41341 tggacgccca taaactagca gggcggctac atttgtagaa agaaatttgg gtttggacat
    41401 gttttttgtt agataaaata acttattctg caaaatctgt ttctaccatt ttaccagcaa
    41461 caacaattat tttaatagtc ctttaaccga tgtgcaacat tgaagacata aagcagaagt
    41521 gcggccctta tatatacgcc gttacgcaat tgcctccagt acatggctcg tgggtctgca
    41581 tctacctcag ttgaaatttc gtgcctcctg ggtaaagggt gcaaaataat ggcatctgat
    41641 ttcatttgat ttacattgtc ggtcgtcaaa ctaaattggg aataatctat caggttggat
    41701 tcaccagaaa gatcatactc atcctgaaga cgggtcatat aaacacaatc cacatctgaa
    41761 agaatactgc ccaagtcatc agttttgcga attttgatgt tttgagaacg caaaatatcc
    41821 tctaaatctt ctgcaatctg aaaagacgat ggtgcaacaa aatccatctc gacatcttta
    41881 tattgactca ataacatcac tgaagagcgt actgccctgc cacgcaacag gtcacccaca
    41941 aacgcatagc gttttttact gattcctccc ttgtgctgaa atgatctgtg catagtgtaa
    42001 aggtcaagaa gagcctgagt cggatgttga tctttaccgc tgcctccatt cataatgggc
    42061 acgctgcgtc cagtattatc gagcaaataa gccatatatt cgcaaaatcc cggtttcgga
    42121 tcacgcatga taatcaaatc aaaatagctg ctgaaaaccc taactccgtc ttcctgactc
    42181 tcgcctttgt attcagacga caaagaggga tcgcggactt ctccggtgtc cattccaacc
    42241 atctggcatg cagtcaaaaa agacaaaaag gtccgtgttg agggttgggt aaaatagagc
    42301 attgcctttt tgtggctcaa taaggatttc agaaattcta tcccgtttgg tttgcgagtc
    42361 attcctttaa cgtgatccgc aatgtcaaat aattcttcca tcaacttaat ggagaattga
    42421 tgcgagagca tcacatgctg gagacgtcca tctttttcat aatgctgcat gcgtacttcc
    42481 actgactgtg aaaagtactc ttcgaaggtc tttagtgagg attccatgag cgtaaaactg
    42541 ctaagatgta attagaaaat aagttgtaat ctagcctaaa attctattca gaaacagtcc
    42601 agcggaaagg gaatgaatca attttttcag cagtaataaa tttttttctt gggaggtatt
    42661 ttttgagaat tataagcggg caggcagtaa ttgcaaagta tcgtgcaact aaatagagcc
    42721 gtgtccattt aaggacacgg tgataaaaaa tattacttga tttctgtgct tgctccggct
    42781 tcttcaagtt tctttttgac ttcttctgct tcttccttgg aaacaccttc tctaatagct
    42841 ttaggagcag attcaactgc ttctttagct tctttcaatc ctaatccagt aattgaacgg
    42901 acttccttaa tgacgttgat tttcttctca ccaaagctgg taagtattac atcaaattca
    42961 gttttttctt cagcagcggc ttcactctct ccaccaccgg cgacaggtgc cgcgactgca
    43021 gctgcagctg cactgacacc gaatttttct tccatctctt tcaccagttc agacatctcc
    43081 agtacggaca tgtttgcaat gaaatcaact acttggtctt ttgttatatc tgccatttta
    43141 tatctcagat aaaggttaaa gtcatcctcc attttagcta agggaggaat gtgggaaaaa
    43201 ccggtttagt tatttgctat cccggattgc agccaaagtg cgaacaaatt tcgcagggac
    43261 tgcgtttaga gtacgaacaa attgagttgc tggagcctgc ataaggaaca gcagcttcac
    43321 gatgagctct tctcttgaag gtagtttgga aagtgcttcc acttcaccag tattgaggat
    43381 acttgttttg ctgtctccaa caagtattcc tgcaaggatt tttaaattat caatattttt
    43441 agctgcctcc gacaatactt tggcctgcac aaccggatca ccgtcactat atactaacgc
    43501 acgagtttgt gtgaactgat ctgcaagctc cgaaaacgga gtgtcttcgg ctgcaatacg
    43561 tgccagggag tttttgagta ccttcattgt ggaagatgca tcattcaact gcttgcgcag
    43621 ttccactacc tgattggctt caattcctcg gtaatcaacc agcactccag ctgacatatt
    43681 attaaatatt gatcgaagct cgctgacttt agcttccttt tgacttcgtt ccatggcccc
    43741 tttccaaatt gattaagtta aaaattcact tctccggtgt tttacacaag tgcgaaaatt
    43801 cacattattc tcatagaaaa ggctaaaacc catttctaag aaaactgaag attgtgatgt
    43861 aaaaaatttg aataagacag tggaaggaac acagcagaaa atttctgtgt cactcgcgga
    43921 gaggtgtctc ggcagggtat aaattaagcc gttaggcccc tactgtctca gacaatcata
    43981 ctaaaaaaat tatcttttat actgtttgtg gatccacttt aattcctgga cccatagttg
    44041 aactcaaact aaaatttctc agataaattc cttttgaaga ggatggtttc atcctcttga
    44101 cggtgtcaac caaaacactt atattttcac agagcttatc tgcatcaaac gaaattttcc
    44161 caactgaagc atggagattg cccagtttat ctgcacgaaa ttccacttga ccggctttga
    44221 tattttttat gacagattca acctcaaaag ttactgtacc agtctttgga tttggcatca
    44281 accccctcgg accaagaatt tttcctattc ggccaactac tgccatcata tcaggcgttg
    44341 caaccacacg atcaaactcg agccagccat cctgaatttt tttggcaaaa tcttctccgc
    44401 ctacataatc tgcacctgct tcttgcgctg caattgcctt ttctcccttg gcaaaaacaa
    44461 gcacgcgtac ttccttacct agaccgtttg gcatagaaca ggtcccgcgc accatttgtt
    44521 ctgctttacg ggtatcaact ccaagcctta aggctacatc tacgctttca tcaaatttag
    44581 ttgctttgac ttctttaagc aaattcactg cttcagacag cgtatactct tgttcggata
    44641 ctactttatc ttgaataaat tttgctcgtt tactttgttt actcatattt cctcagtcaa
    44701 tacgcccatg ctccgggctg ttccagccac agacttcaca gcagcttcca agttgtaaca
    44761 attcaggtca ggcatcttct gcttggcaat atcctctatc tgtgctttgc ttagagtacc
    44821 aacttttgac tcatgtggtg ttgccgagcc tttgttcagt ttaagtgcct ttttgatcaa
    44881 aactggtgca ggaggagtct tggtaataaa ggtaaacgat cgatcattgt aaacagtaat
    44941 caccaccgga ataatggtat cctgaccttg tgtttgggca ttgaaggctt tacaaaactc
    45001 catgatattc actccatgct gacccaatgc aggaccaata gggggtgatg gatttgcttt
    45061 accagccgga atctgaagtt tgataaaaga agaaatgtcc tttgccatag ttatctgtct
    45121 tattctacta catgttttac attttcgaac tccacctcta ccggtgtggg acgaccaaag
    45181 atactaacta atacctttaa tttcctgcgt tcgagattta cctcatcgat tgttccactg
    45241 aaattcccga atgaaccttc tgtgattgta actttctgtc cgactgagta ttcatcctca
    45301 gtcgcttttt gctgaaaacc ttcattaacc tgggatcgaa tctcttccag ttccttctca
    45361 tctaaaggta gaggtttttt tttagttcca ccaataaatc cggaaacccg gttaacgttc
    45421 atcaaaaggt gccacaattc aggtgttaac tccatgtgaa tcagtacata tccgggaaaa
    45481 tatttttggt tgactttcct ttttttacct tccttggtac gagttatgtc ttctgaaggt
    45541 ataaaaatct catcaaccaa atgttctatt cccttaattc ttaatttttc tctaattgtt
    45601 tgttcaacac gcccttcgta acctgaatat gcctgaagaa tataccattt tacgttcgga
    45661 ttttgtggtt tttctgctac ttgtgattct gtttcttcgg tttgattttc tgtttccatc
    45721 gaattttcaa cagtttctac catctcggtt gtagcagcat cttcctgctc tccaagatac
    45781 gcatcactta tttgaggtgc gttttcctgc acttctagat ccacatcagt ctcttccgaa
    45841 agattatcct cctgatcatc cgaattgatc tcctgcgttt ccgtattttt ttcctgttct
    45901 tcaaataaca tttctgttcc agtttgttct tccaccattt tatccggcaa taatcatttg
    45961 cataatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct gttccagttt
    46081 gttcttccac cattttatcc ggcaataatc atttgcataa tgtctgaaag acccaaatcg
    46141 gcgattccca aatatagggc cacagcaaaa gagagacaaa caaccactga tgtggacttt
    46201 attgtggcat cacgtgtcgg ccactgcacc cgcttaaatt ctgcaatgac atctttaaaa
    46261 aactgccgaa ttcttttgaa cataatcagg ttcgcgattg aattagaaac attctaaatt
    46321 aatattcttc tgctgttctg cagtccaaac ctttaagtca aagcaggtgc tgacaatttt
    46381 ggcagggcag acaggggtcg aacctgcgac cttcggtttt ggagaccgat gctctaccaa
    46441 ctgagctact gcccttttcg gacgtaaaat aaaggcagaa aacgaattat ttcgtttcct
    46501 tatgtaatgt atgtttgcga tcccacctgc agtatttacg tacttccata cgctctgttt
    46561 ttaatttttt gttgcgggaa gtagtataat tcctgcgttt gcactcagtg cattccaagt
    46621 ggatcgtgtc tctcatattt tcattacctt aaaaaactac tataataaat tggagcctga
    46681 gagcaggatt gaactgccga cctcttcctt accaaggaaa cgctctacca ctgagctacc
    46741 caggcttaaa actcatactg ttataagctt ttgaagctgt ggtggggaga gaaggattcg
    46801 aaccttcgaa ggcgtagccg tcagatttac agtctgatcc cgttgaccgc ttgggtatct
    46861 ccccatgctg gagctggcaa taggacttga acctacaacc tgctgattac aaatcagctg
    46921 ctctaccagt tgagctatgc cagcatgagt taaacggtta attatgtaat gccagttgat
    46981 atttgtcaat ataaaacata aattatctgt ttctaccaca ttaaaaatgc gggcaaaatg
    47041 taatcaggat acttgaaaaa taaacagtaa ttaaattatg cggcggccaa atagatttta
    47101 gaatcagctg attttttccg gtcaggaagt ttaatatcgt gatttttgct gaattgataa
    47161 tcttgaaaaa cttcttcagc acttagaagt tgatatttcc catcctc
//
LOCUS       DTVD01000241            4774 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6138_length_4774_cov_275.316381, whole genome
            shotgun sequence.
ACCESSION   DTVD01000241 DTVD01000000
VERSION     DTVD01000241.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_4774_cov_275.316381"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            130..1896
                     /locus_tag="EYO68_08905"
     CDS             130..1896
                     /locus_tag="EYO68_08905"
                     /inference="COORDINATES: protein motif:HMM:PF06315.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIC07956.1"
                     /translation="MTQSENTHFKGLIEKASDSKKIDLLAQWALCEFDLFYSSFQTIT
                     ESAKTAFETRDYQASLTISRKRLSLYSDSMFKLGEKLADAFSPISRDQALWEVIEEKY
                     RQLVRNRYEGDLALAYIHSVRRSLFLGEWSPVAYSFDTPSKANTNFSNFLFETFQTEL
                     ITTDLLINILRVPGFKVQYRELKVDAMLAAQRVKADLDDRFSTYKIQKIEVIKGEFYR
                     NRGAYIVGRIVLDNHSKVPLVIALLNDEKGIYVDAILTSESATFNIFSTTRANFHVNN
                     EYYHELSEFLHSIIPKRSLGLAYSTIGFNHFGKVAVMEELKEELLSTDGKLDFAIGFK
                     GTVAIGFQSPQSGYNLKVIRNTPTEQYKWGVFEGVPSVLEKYGRVHVINRTGSMLDNI
                     IFYRVKLERAWFTNALLQELLNDASECVTLQGESLFFRHLIVQSKLIPLPVYLENSSQ
                     AESEAAIINLGHCIKNNMAANIFNKDLDARNYGVGVFGGVYLFDYDALEQFTEVKIRT
                     NQNQFEGEEDIPEWFFEDGVIFLPEEIESGLRIPNRSLRQLFREVHGDLLQVDYYERI
                     QDELGVGKVPSVRVYPERYQIN"
     gene            2192..3199
                     /locus_tag="EYO68_08910"
     CDS             2192..3199
                     /locus_tag="EYO68_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIC07957.1"
                     /translation="MKVCIVGAGAIGGYMAVRIANAGHNVSVIARGPHLAAIKARGMK
                     LIEENDEFVAENLTATEFVGELGPMDVVLLALKAHQIAPIVNDMSVLLGPNTVIVTLQ
                     NGIPWWYFQNFVGDYANRVVETVDPGGLLFKSIDPDRLIGCIAYPAATISKPGVIQHI
                     EGNRFPVGELCGMKTERVQMVSDLFTESGFKSRILDDIRSEIWLKLWGNLTFNPISAL
                     SHSTLVDICQFPLTRQLAATMMTEAQTVGERLGAHFRIPMEKRIAGAESVGKHKTSML
                     QDVEAGKPMEIESMLGAVIELAEVTGVQTPTLSAIYACVSLLNKTLSQEKIIIKGISK
                     E"
     gene            3489..4268
                     /locus_tag="EYO68_08915"
     CDS             3489..4268
                     /locus_tag="EYO68_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HIC07958.1"
                     /translation="MTQWIRFDYQEKTEIGTLTGDTVSEYKGGLFDRPQPTGRSMPLA
                     DVQLKAPLVPHSIFALWNNFHERAQKESQTLPDVPLYFMKPITSVIGPGQTIYRPRGH
                     HGRVIFEAELGIVLGSTCREISEEEAGQYIFGYTCVNDVTAIEFLFEDKAFQQWTRCK
                     GFDTFTPLGPWITTGIDPGGLQVMALQGDKVRQDYPVSDMIFSPMKIVSLMSHYHTLL
                     PGDLICCGTSVGARTMKPGTSIDIVIPGIGKLSNPFDDLSG"
     gene            4492..>4774
                     /locus_tag="EYO68_08920"
     CDS             4492..>4774
                     /locus_tag="EYO68_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIC07959.1"
                     /translation="MSRAFRQNTTQKNTYYPQLLGTHGAVATEHYLATKAGADILGGG
                     GNAVDAAVGATFVEGVVNPQMFTLGGECPMLICMAETNQVVSVNGNTMAP"
BASE COUNT         1371 a          992 c         1070 g         1341 t
ORIGIN      
        1 aaagagaagt gaatcgagaa gaattttaaa ctctttttga acaaaccgat gaccatgttt
       61 tgagcttgat agataaatgc attttctcat ttaatttaat tttaattcaa atatacaata
      121 atcagctaca tgacccaatc agagaacact cactttaaag gtcttattga aaaggcctcc
      181 gacagcaaaa aaatagatct tctggctcaa tgggctcttt gcgaatttga tcttttttat
      241 tccagcttcc agacgataac agaatcagca aagactgcat tcgaaactcg cgactaccaa
      301 gcgtcgctga caattagtag gaaacgactg tctctataca gtgacagtat gttcaaattg
      361 ggggaaaagc ttgcggatgc tttctctcct atctcaagag atcaggcact atgggaagta
      421 attgaagaga aatatcggca gcttgttaga aaccgttatg agggggacct cgctctggca
      481 tatattcatt cagttcgtcg gtcgctcttc ctaggagaat ggagccccgt agcctattcc
      541 ttcgataccc caagcaaggc caatacaaac ttttctaact ttctgttcga gacttttcaa
      601 acggaattaa tcaccactga ccttcttatt aatatactcc gtgtccctgg attcaaagtc
      661 cagtatcgtg aactgaaggt ggacgcaatg ttggcagcac aacgagttaa agctgatctg
      721 gatgacagat tcagcacata taaaattcag aaaatcgaag tcattaaagg ggagttctac
      781 cgtaatcggg gtgcctacat cgtcggaaga attgtacttg ataatcattc aaaggtacca
      841 cttgtcatcg cgttgctgaa tgatgaaaaa gggatttatg ttgatgcgat tctcacttca
      901 gagtctgcca ctttcaatat tttcagtaca acccgggcaa actttcatgt taacaacgaa
      961 tactatcatg aactttcaga atttcttcac agtattatac caaaacgatc tctgggtcta
     1021 gcctattcta caatcggatt taatcatttt ggtaaagttg cagtaatgga agaattgaag
     1081 gaagaactct tgtctactga tggaaagctt gattttgcta taggttttaa gggtactgtt
     1141 gcaataggtt tccagtcccc tcaatcgggt tacaatctta aagttattcg taatacacct
     1201 actgagcaat ataagtgggg agtgtttgaa ggagttccct ctgtgctgga aaaatacggt
     1261 cgtgtccacg ttattaatcg gacaggcagt atgctggaca acattatttt ctatcgcgtt
     1321 aaacttgaaa gagcttggtt caccaatgca ctactgcaag aacttttgaa cgacgcgagt
     1381 gaatgtgtga cactgcaagg tgaatctcta tttttcagac accttatcgt acagagtaaa
     1441 ttgatcccct tgccggtcta cctagaaaac tcatcgcagg ctgaaagcga ggccgcaata
     1501 atcaatcttg ggcactgcat caaaaacaat atggctgcca atatattcaa taaggacctc
     1561 gatgccagaa actatggtgt cggtgtattc ggaggagtgt acttgtttga ttacgacgct
     1621 ctggaacagt ttactgaagt taagatccgc actaatcaga accagttcga aggagaagaa
     1681 gatattccag agtggttctt tgaggacggt gtgatattcc tgcccgagga aatagaatct
     1741 ggcctgagaa tacccaatcg ctctctgcga caattattta gggaggtaca tggcgacttg
     1801 ctgcaagtag attactatga gcgtattcag gatgaactag gtgtgggtaa agtccccagt
     1861 gttagagtct atccagagcg gtaccagatc aattagaaag tctatgcaca acattgcaag
     1921 ggtaattgag aatcaccctt tcggagtatg agacgatcat gtctgtctct tgcttaataa
     1981 atatcaagag aaattcattt tgttgatgac cttcatccta tttattgcaa taatacacga
     2041 ttaaaatcgg ttagcttata atcctcagtc gtacagttac ttaaacttgt tccaacgttc
     2101 acttctttca ttcagcgaat cgagagtaat tattagattg tttcattgca gggcacagct
     2161 tgacttgtcc atctcataag gagtttacag catgaaggta tgtattgttg gagctggagc
     2221 catcggcggt tacatggctg ttcgcattgc aaatgcagga cataatgttt cggtaatagc
     2281 acgtggtcct catttggctg ctataaaagc cagagggatg aagttgatcg aggagaacga
     2341 tgaatttgta gctgagaatt tgaccgccac tgaatttgta ggggaacttg gaccaatgga
     2401 cgtggttctc ttggcactta aagcccatca gatagcccct attgtgaatg acatgtctgt
     2461 acttcttggc cccaacactg tcattgtcac ccttcagaat gggatcccat ggtggtattt
     2521 ccaaaatttc gttggtgact atgccaaccg ggtagtggaa actgttgatc ctggcggttt
     2581 gctatttaag agtatcgatc cggatcgctt gattggctgc atagcctatc cggccgcaac
     2641 tatttctaaa ccaggagtga tccagcatat cgagggtaat cgttttccag tgggagaact
     2701 atgcggcatg aaaacagagc gagttcagat ggtgtctgac ttatttactg aatcaggatt
     2761 caagtctaga attctcgatg atattcgctc tgaaatctgg ttgaaattgt ggggaaatct
     2821 gactttcaat ccaatcagcg ctctctccca ttcaacgctt gtcgacatat gtcagttccc
     2881 gttgaccaga caattggctg ccacaatgat gaccgaggcg cagactgtcg gagaacgcct
     2941 gggggctcat ttccgcatac ccatggaaaa gcgcattgcc ggtgcagaga gtgtcggcaa
     3001 acacaagacc tcaatgctcc aggatgtgga ggctgggaaa cccatggaaa tagaaagcat
     3061 gctaggtgct gtgattgagt tggctgaagt tacaggtgtg caaaccccta ccttgagcgc
     3121 tatttacgct tgcgtgagct tactcaacaa aacactaagt caagaaaaaa tcataatcaa
     3181 agggatatcg aaagaataaa cctaattttt ttaataaata agttgtcaag aaacaaggtt
     3241 actcgttttt ccatcaacga agttctcgat attatcaact acttgattag acgattgttt
     3301 gctacctaga tccagcggtc tgcgtaacta catacaaata tactacggca tcttgctggt
     3361 gtttgtatat ttttggtgtt caaattgaga ctgctaaacg aactttacgc tattactgat
     3421 ttagtaatgt gccgactgcg gcaatgggaa tttttgggtt ttttacacta aaaggagact
     3481 taccacaaat gactcaatgg atacgttttg attaccagga aaagacggag atcgggacac
     3541 tgactggcga tactgtctca gaatacaaag gaggactttt tgatagaccc cagcctacag
     3601 gccgttcaat gccacttgca gatgtccagt tgaaagcccc gctagtaccg cattccattt
     3661 ttgcattgtg gaataacttt cacgaacgtg cccaaaaaga aagccagacg cttcccgatg
     3721 taccacttta cttcatgaag ccgataacca gcgtgattgg tcctggacag actatctatc
     3781 gtccccgcgg acatcatgga cgggtaatat ttgaggctga attgggtatt gttctcggct
     3841 cgacctgccg agagatcagc gaagaggagg ccggccaata tatttttggc tatacctgtg
     3901 tcaatgatgt tacagcaatt gagtttcttt ttgaggacaa agcattccag cagtggactc
     3961 gctgtaaggg gttcgacacg tttacaccgt tggggccttg gataaccact ggaatcgatc
     4021 caggcggcct tcaggtcatg gcgttacagg gagataaggt acgacaagat tacccagtta
     4081 gtgacatgat tttttcacct atgaagatcg tctctctgat gtcgcactac catacactac
     4141 ttccgggtga cctaatctgt tgtgggacgt cagtcggtgc ccgcaccatg aagcctggta
     4201 caagtattga tatcgtaatt cccgggattg gaaagcttag caatccattt gacgatttat
     4261 ccggatagta gaattttgtc aggaactatg actgtgaaga attagtggag ttaatcaaac
     4321 aggaactgag tttcccttat ccttaccatc tcacgtaaaa tacaacaaat gttgatagat
     4381 tttaaacaat ttgaaaaaat ctcaacccca catagctagg attgattttt tgttggacta
     4441 cagaatttat ttagttgtct ctatttttct aaacttaatc atgaaaccag aatgagccga
     4501 gcttttcgtc aaaacaccac ccaaaaaaac acctactacc ctcaacttct aggaactcat
     4561 ggtgccgttg ccactgaaca ctacctggcg acgaaagccg gagccgatat tcttggaggc
     4621 ggaggaaacg ccgtggatgc ggcggtcgga gcaacctttg ttgagggagt agtcaatccc
     4681 cagatgttta ccctcggagg ggagtgtccg atgttgatct gcatggcaga gacgaaccag
     4741 gtagtttcag tcaacggcaa caccatggct ccgg
//
LOCUS       DTVD01000242           17124 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_615_length_17124_cov_268.357842, whole genome
            shotgun sequence.
ACCESSION   DTVD01000242 DTVD01000000
VERSION     DTVD01000242.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17124)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17124)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17124
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_615_length_17124_cov_268.357842"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="EYO68_08925"
     CDS             complement(<1..434)
                     /locus_tag="EYO68_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07960.1"
                     /translation="MSEEKIDGLEGLDDFGDDFGEQLDSFMENDGEDEGDSELDSFFE
                     DLSTIDDLESDDNSESEETKNDDNEDDQNNDDDDVQVDDHNHDDDDVQEEDHNLGDDD
                     LDEVDDDDGLGDEEIDDDEEKQEQESKRKAASKGFPLKAVILS"
     gene            complement(644..1441)
                     /locus_tag="EYO68_08930"
     CDS             complement(644..1441)
                     /locus_tag="EYO68_08930"
                     /inference="COORDINATES: protein motif:HMM:PRK13321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIC07961.1"
                     /translation="MILAADIGNSDIVLGIFKDSELLQNWRLHTVHHQSVDEYEMLVR
                     GMLFACDFSLEDVDGAVLSSVVPELTNVFLNLIENLVGQPPLIVNSDLNFGIEINTEI
                     PAKVGQDRLINALAAYQQYKTSLIIIDCGTATTLDVVTAEGSFEGGVICPGLLISAEV
                     LFSRAAQLFQVNLEIPENLIGKNTTDSVKSGLIYGYGGMIDSLIDKLAKEIVQMDQPY
                     PTVVITGGLAKKLLPILPEVIFHDDLTLRGLYLFHLMNKPVENGKEQ"
     gene            complement(1446..2291)
                     /locus_tag="EYO68_08935"
     CDS             complement(1446..2291)
                     /locus_tag="EYO68_08935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIC07962.1"
                     /translation="MSENIGCHIIRLKEIDSTNSYLKDKSELLQRNGLVVIAEMQVSG
                     RGRAGRKFTSVIGNNVTFSVVLHPNLPLEEIQIFALLAGVAVARVLENYVKNIRLKWP
                     NDVLVNEKKICGILLETINIPDHSFPVLIMGIGLNTKGCPNDYPEELQNIVTTIESEM
                     SPISDISSKTESAPVLNNESIFQQLLLELDSCLEKFSLVSANTQNSDKTTSGRSSLLQ
                     EWLQRAKALGRKVCSLNNVEERVKDSGTVGIIEGLTREGYLQIRTDSGQILTHVSGDI
                     FDMKR"
     gene            complement(2298..3491)
                     /locus_tag="EYO68_08940"
     CDS             complement(2298..3491)
                     /locus_tag="EYO68_08940"
                     /inference="COORDINATES: protein motif:HMM:PF03934.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07963.1"
                     /translation="MSHLLVTPPSHLDKSPQRGIALLITLVILALLAVFMTEFSFETK
                     LETRGIQNYQASFKARNAVKSMFKAVLEGLKGQNEVKFFRVYLQGLQYNFSEFSGKDC
                     TLLNPCKPVKLIAGVIADFPDVIFYTPYIRPIDHLFNLNRIRKNNPGTPEDLDLHNQF
                     VNILKNMKSVSGQVSLGISSIDRIYAALFDWTDGDQEPYEMIGIEHYQDLQTEYEIKN
                     QKLDKLSEILLIDGIAESGIPLDNSQSGPGNWKKYFTVFPVENRAEGFEQPRINVNLA
                     TENEIVDFLMQFENQADSSSSQTFIAKAIEIGSTLKLITSGEKGKTYYSSSEIQKSLD
                     SSPATSDLPVVAKQFFIPYSNWYEINLKTEIENVQAEVNAFVSVNRKPDHSVEKLIIH
                     EFLLR"
     gene            complement(3629..7327)
                     /locus_tag="EYO68_08945"
     CDS             complement(3629..7327)
                     /locus_tag="EYO68_08945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIC07964.1"
                     /translation="MKIKRLFTMEGESPYGSIEFENRASVIRNPDGSIVSKWDNVSVP
                     KHWSQVATDILAQKYFRKAGIPKHLKSAKEKGVPDWLQPSLYDQAKADQEASEADSSA
                     VDTTVSETDAREVFHRLAGCWTYWGYKHNYFDTEEDARAFYDELCHMLANQMAAPNSP
                     QWFNTGLNWAYGLSGPAQGHFFVDPKTEEVMPSKDAYTRPQPHACFIQSVKDDLVRDG
                     GIMDLWTREARLFKYGSGTGSNFSDLRGDAEPLSGGGVSSGLMSFLKIGDSAAGAIKS
                     GGTTRRAAKMICLDADHPDIEQFINWKVHEEQKVAALVSGSKTIKRLIKELFEAIHGW
                     ENESEKFDIELNKDLRSVVKASRLASMPFSYVYRIIHLSKQGYTGVLFDEYDTDWNSE
                     AYQTVGGQNSNNSVRLNNAFMQAVEQGKDWNLYWRVEKVKAEKEGREPVPCKKLKATE
                     LWEEIAYAAWASADPGIQFDSTINEWHTCPADGPIRASNPCSEYMFLDDTACNLASLN
                     LLQFRDPKTGELDIVKLRHGSRIWTIVLEISVLMAQFPSRNIADLSYEFRTLGLGYAN
                     MGTYLMVNGIPYDSAKALAICGAVTSIMHMTSYATSTEMAKELGTFSGYEKNKDNMLR
                     VIRNHRRAAFRAPDSEYEGLTIKPVGIDSQHCPPELLKAAQQDANTALKLGEKHGFRN
                     AQVTVIAPTGTIGLLMDCDTTGIEPDFALVKFKKLAGGGYFKIINNSIPPALYKLGYD
                     DSEVSDIVHYIKGHARLDGSPCINANILRTKGFTSEVLKRIEEQLPTAFDISFVFNMY
                     TLGESFCKETLGLSDEQLGDFDFSILKHLGFSSKEIEAANDYICGTMTIEDAPHIKPE
                     HYSIFDCANKCGRKGKRFIAPEAHIRMMAVAQPFISGSISKTINMPNEVNITDIKEAL
                     SSSWHYGLKCNALYRDGSKLSQPLNVNVEEYLWDEDEVEGDIAAEKPEQVRIAEKIIH
                     RYIAKRRKLPHRRSGYTQKAVIGGHKLYLRTGEYEDGTIGEIFLDMHKEGAAFRSLMN
                     SFAIAISLGLQHGVPLEEFVEAFIFTRFEPNGMVSGNDSIKMSTSIIDYIFRELAISY
                     LGRNDLAQVNADELQASAVHTQNEDQPEFDNEQVVSERTLSTEESLAFLEQEESDFEG
                     VDFALLKNSDPKPEQVHAEFTGNSDFGTDTSTESWETQERSTTTLVQEARMKGYEGEA
                     CHTCQQFTMVRNGSCLKCASCGATSGCS"
     gene            7841..9031
                     /gene="hemW"
                     /locus_tag="EYO68_08950"
     CDS             7841..9031
                     /gene="hemW"
                     /locus_tag="EYO68_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIC07965.1"
                     /translation="MQKKRSPIPAVYLHIPFCRTICPFCSFTVRRDRAKMHEKYIRGM
                     VGEIDRRAEMLKDMERKENEEKLSGQILLKSIYIGGGTPSCLTIPEVSVLLTKVRENF
                     PWSDQIEIAFEMNPDDVNPEYLSGLADIGVNRLSLGGQSFQTSTLQKLGRCHTVADLR
                     NAIAVITNSSFDNWNLDLMFGIPEQSIVMFKNDVEEALRYEPSHISLYGLEIHERTPF
                     GNNPQIRNWEPEHLEQFEEMYLWATDNLEKAGLFQYEISNFSKKTNEGRQNLLVWSGQ
                     EYLGFGVGAHSYHRQTRWGNVRSIRTYLQHLGQNTWPTEFIEQLSGKQLAVEFLMLGL
                     RQCEGINIKEWQTRYGLHLQNQQLNFVQELCDDGRAFWEGQHLCLTPKGMLLADRITV
                     QLMP"
     gene            9188..10798
                     /locus_tag="EYO68_08955"
     CDS             9188..10798
                     /locus_tag="EYO68_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIC07966.1"
                     /translation="MRIRSNATSLNTLRHSDNNLRNVRSSIEKLSSGTKINSAADGPA
                     SLIASERLRGQIAGLRQAYSNNENAIAMFQTAEGALSEASNILIRLKQLSVHAANEAV
                     NDDSMLAADQHEVENLLSTLDRIVKTAEFNGRILLDGSMGANGASVGNNIRFVNAETW
                     TEASPMEGYEVDITQVATQPYIRGSVPLTVQNIGEGVKILLSEGGRNVEVDTRFGKVK
                     ENIEEVLSNYKQDPSRFPAEEASKNIRAIVINSINEAIEGSGLALEAFETPENTFYIR
                     HKDFGASPTFSLTTNISGLLTKESNVAESSIPGLDVAGKIGDSFTNGKGQFLTAIKRS
                     SPAQGIAIQYDRSIGLKEVPVKNEQGEVVGTEFVEETQEEIVGSPSNPIIEGYVHISQ
                     QTKNVGLGTKPGADKGFSLKNIRTNNLALGVENESGFRSLFDIDLTSKQGADNSGRLI
                     EKAIDEISVLRGEIGSFQKNTVESNLNSLRVAEENISRGESIIRDTDMAAEMSKLTGN
                     QILLSASQSMQAQANQLPQNVLQLLQQQ"
     gene            complement(10853..11554)
                     /locus_tag="EYO68_08960"
     CDS             complement(10853..11554)
                     /locus_tag="EYO68_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07967.1"
                     /translation="MLELKHLETSYGQSQVLFGVDLQVNEGEVVTLLGRNGMGKTTTI
                     NSIMGLVRARSGEIKFEGSQIREWPTHRVAQSGMGLVPEGRQIFPNLNVIENLVATSA
                     NRFKNKNPWTLDRVFNLFPSLLERAKNMGNQLSGGEQQMLAIGRALMTNPRLLILDEA
                     TEGLAPLVKMEIWECLNGLKTSGQSILVVDKNIEALTHLANRHYIMEKGKIVWSGDSP
                     ALIADKEIQHRYLGI"
     gene            complement(11547..12311)
                     /locus_tag="EYO68_08965"
     CDS             complement(11547..12311)
                     /locus_tag="EYO68_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC07968.1"
                     /translation="MKRLIKMLKVDSLFKSFGALLATDNLSFQVKKGDIHAIIGPNGA
                     GKTTLIGQLTGEIESDSGSILFDGNNISGVPVHLRSAHGLARSFQITNIFHDMTTWDN
                     VALAVQAHAGHSFHFWKNASQEQELRRPAIEFLAMVGLEKRAGFIAGLLSHGEHRQLE
                     IAMALATNPKMLLLDEPMAGMGPKESLAMVDILIKLKHKLTILLIEHDMDVVFTLADR
                     ITVLVNGRGIATDTPDAIRKNPEVIAAYLGEESYHA"
     gene            complement(12295..>12825)
                     /locus_tag="EYO68_08970"
     CDS             complement(12295..>12825)
                     /locus_tag="EYO68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07969.1"
                     /translation="NGLIDISNDNSLYYLNFVCLLLSLYLSHRLINSRFGMLIRGAKS
                     NEQRMRAIGFPVFRYQLVAFVIAGTICGIAGVLSANQTEFVNPSVMHWTHSGELIIMT
                     VLGGLGTLFGPVIGAVAFLFLEEFLSGITEYWQLFFGPMLVLVVIFARGGIDGMLVGL
                     DQWWSRNSPVKNEEVD"
     assembly_gap    12826..12925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12926..13451)
                     /locus_tag="EYO68_08975"
     CDS             complement(<12926..13451)
                     /locus_tag="EYO68_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07970.1"
                     /translation="MKWSPRIIFIVILMLGLGVVPLLADYFGEPFYTVMFSRMLILSI
                     GAVSLNLILGFGGMVSFGHAVYLGIGSYMVGIGTMHAVEDGIEWMANGFLQIALAIVF
                     SALAGLVIGAISLRTRGVYFIMITLAFSQMLYYVAVGNEKYGGDDGLSLYMRSDFNGL
                     IDISNDNSLYYLNFV"
     gene            complement(13452..14375)
                     /locus_tag="EYO68_08980"
     CDS             complement(13452..14375)
                     /locus_tag="EYO68_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC07971.1"
                     /translation="MNWTLLLEQTLNGFQFGILLFLLAAGLTLIFGIMDLINLAHGSL
                     YMMGAYFAATFIKWTGSFLLGAILAIPACMAFGMIVEMIALRTLYTRNHLDQVLATFG
                     LILFFNEFVRLLWGPIGLDIPLPEVLNGSVELFLGIRYPAFRLVIIAVGILVAFLLYL
                     LVSRTKLGMLIRAGASNRQMVGALGVNINLLYTFVFGLGAALAGLAGLMAGPIMTVEI
                     GMGEGILIITLVVIVIGGVGSIKGAFVAALLVGLIDTVGRSFLPDILGIFFPNDVADT
                     AAPAISSMLIYLLMALILAVKPQGLFPPRGA"
     gene            complement(14466..15638)
                     /locus_tag="EYO68_08985"
     CDS             complement(14466..15638)
                     /locus_tag="EYO68_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC07972.1"
                     /translation="MRLGILGTLSIGVLLVGQVALAETVKIGFVTTLTGGAGAIGNDM
                     RDAVELALDHLGRKMGNSDVKMFYEDDTFKPAVGKQKTEKLVKKDKVHFVTGYIWSHV
                     LMASRNSVVGKGPFMITSNAGPGPVAGKLCHEDFFSTSWQNDQTTMAMGEVLNQLGIK
                     NLYIMAPNYAAGKGMVAGVSRTFKGNIVGKDMTKFPAQLDFSAELAKIRAAKPDAVFV
                     FYPGKHGIQFFKQFSQAGLKGTIPLYSAFTVDSLSLPRLKDLAEGSLMTQFWAPDLDN
                     AVNKRFVADYRKKTGRYPTFYAAQSYDTIMLINSAVTAVGGNMSNKDGMRTAMRKANF
                     PSVRGPFKYGNNHFPIQNFYLRKVIKDAEGNYTTTIIKTVYTDHQDPYAKDCKMSW"
     gene            complement(16001..16759)
                     /locus_tag="EYO68_08990"
     CDS             complement(16001..16759)
                     /locus_tag="EYO68_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07973.1"
                     /translation="MPSAVTSPEEDVTLHCPSCGEEKFLFVPMSGKNGERFVRIHCHK
                     CQEHLFFQNPELPNNIKSPEDISEQWQTEQINNLQLEWEEWFRWKKRHLDKSSSRKTP
                     LSKYAQWFLALLLGGSTLFFVLDRWHLLDPLLENPAARQELITAYAERLLEVPCFPSI
                     MKVKIRTVPIRYTRERPVHRNWIQYGEAGIYWGEEQIKIHRSNFWFFGWPKKTQLLNT
                     LIHELRHRSSPGLGHNLKFFNLVERDTSCVLEYW"
     gene            complement(16793..17081)
                     /locus_tag="EYO68_08995"
                     /pseudo
     CDS             complement(16793..17081)
                     /locus_tag="EYO68_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
BASE COUNT         5107 a         3620 c         3318 g         4979 t
ORIGIN      
        1 gatagaatta ccgcttttag cggaaaacct ttggaggctg ctttcctttt tgattcttgc
       61 tcctgctttt cttcgtcgtc atctatttcc tcatctccca gaccatcgtc atcatcaact
      121 tcgtccagat catcatcacc aaggttatga tcttcttcct gaacatcgtc atcatcatgg
      181 ttatggtcat ctacctgaac atcgtcatca tcattgtttt ggtcatcttc gttgtcatca
      241 tttttggttt cttcactttc actattatca tcactttcca ggtcatcaat tgtagaaaga
      301 tcttcgaaaa atgaatccaa ttcactatca ccctcatctt ctccatcatt ttccatgaaa
      361 gagtcgagtt gttcaccaaa atcgtcacca aaatcatcca gtccttcaag tccgtcaatt
      421 ttttcttcag acatatctca ttttctgttg gaatgttgtt tggtgaattg attaagttat
      481 gtagtcgtaa aaattagatt ttgtcacata atcatcacag atctactaaa gatacagaaa
      541 acttttctat aaaacattcc tctgtgatct ctttatatca gtggttgatc gtatatctgg
      601 ttggtatgtt agtttcagag aagctatcaa aaatgattgt gcattattgt tcttttccat
      661 tttcaacggg tttgttcatc aaatgaaaaa ggtataaacc acgtaaagtc aaatcatcat
      721 gaaaaataac ttcaggcaga atcggtaata attttttcgc taaaccacct gtaataacaa
      781 cagtaggata aggctggtcc atttgtacaa tttctttcgc taatttgtca atcaacgaat
      841 ctatcatacc accataacca taaatgaggc cggacttaac gctatctgtc gtatttttac
      901 ctattaaatt ttctggaatt tcaagattta cttgaaaaag ttgggctgcc ctggaaaata
      961 atacttcggc tgatattaac aatcctgggc aaatcactcc tccttcaaag ctcccttctg
     1021 cagtgactac atccaaagtt gtggctgttc cgcaatcaat aattatcagc gaagttttat
     1081 attgctggta tgcagcaagg gcattaataa gtctgtcttg gccaactttt gcaggtattt
     1141 cagtgttgat ttctatgcca aaattcagat cgctgtttac tattaaaggc ggttgaccaa
     1201 ccaaattttc gattagatta agaaaaacat ttgtcagttc aggaacaaca gaactaagaa
     1261 ctgctccatc tacgtcttcc aagctgaaat cgcatgcgaa aagcataccc cgaaccagca
     1321 tttcatattc atctacactc tgatgatgaa cagtatgcaa gcgccaattc tgcagtaatt
     1381 cagaatcttt gaaaatacca agaactatgt cactgttacc tatgtcagca gctagaatca
     1441 ttaatttacc ttttcatatc aaaaatgtct cctgaaacat gtgtcagtat ttgaccagaa
     1501 tctgtccgta tttgcagata accttccctt gtcaaaccct caatgatacc aactgttccg
     1561 gagtctttta ctcgttcttc tacgttattc aaactacaaa ccttcctgcc taatgcctta
     1621 gcccgctgca accattcctg cagcaatgag gaacgacctg aggttgtttt atcagaattt
     1681 tgtgtgttag cagagactag actaaatttt tccagacagc tatctaattc tagcagaagc
     1741 tgctgaaaaa ttgattcatt attaagaact ggtgctgatt cagttttaga tgatatgtct
     1801 gaaattggtg acatttcaga ctctattgtc gtcacaatat tctgtaattc ttcaggataa
     1861 tcattaggac agccttttgt gtttaaaccg attcccatga ttaagacagg aaaactatga
     1921 tcggggatat tgattgtttc gagcaatatt ccacagattt tcttttcgtt taccagtaca
     1981 tcattgggcc atttaagccg gatatttttg acataatttt caagaacacg agctacagcc
     2041 actcctgcta ataaagcaaa tatttgaatc tcttcaagtg gcaaatttgg gtgcaaaact
     2101 acggaaaaag ttacgttatt tcctattaca gaagtaaact ttctgccggc tcttccacgt
     2161 cccgaaacct gcatttctgc aatcaccacc aagccgttcc tttgcagcag ctcacttttg
     2221 tctttcaggt atgaatttgt tgaatcgatt tccttcagac gtataatgtg acagccaata
     2281 ttttcagaca ttgttattta gcgcaacaaa aattcgtgga ttataagctt ttcaacagaa
     2341 tggtcaggtt ttctgttcac cgacacaaac gcattcactt ctgcttgtac attttcgatt
     2401 tcggttttca ggttgatttc ataccaatta ctgtaaggaa tgaagaattg cttggcgaca
     2461 accggcaaat cagaagttgc aggacttgaa tccaaagatt tttgtatttc agaacttgaa
     2521 tagtatgttt tacctttttc tccactggtt attaatttta gtgtagaccc tatttctata
     2581 gcttttgcta taaatgtttg ggagctacta ctatcagctt gattttcaaa ctgcatcaaa
     2641 aaatcaacaa tctcattttc tgttgcaagg ttgacattta tacgtggttg ttcaaaacct
     2701 tctgccctat tttcgactgg aaaaaccgta aaatattttt tccagtttcc tggtccagat
     2761 tgagaattat ctaaaggaat tccgctttca gcaataccgt caatcagtag aatttcgcta
     2821 agtttatcca acttttgatt cttgatttca tattcagttt gcaaatcctg gtagtgttct
     2881 attcctatca tttcatatgg ttcctggtcc ccatcagtcc aatcgaacaa ggcagcataa
     2941 attctgtcga tggaagaaat tcccaaagag acttgaccgg acacagattt catatttttt
     3001 agtatattca caaactggtt atgcaaatct aaatcttccg gtgttccagg attgttcttc
     3061 cggattcgat tcaaattgaa caaatgatca atcggacgaa tatatggtgt ataaaaaatt
     3121 acatcaggaa aatcagcaat tactcctgca attaatttta caggtttgca gggatttaaa
     3181 agagtgcagt cctttccaga aaattcacta aaattatact gtaatccctg taagtagact
     3241 cgaaaaaatt taacctcgtt ttgacctttc aacccttcca gtacagcctt aaacatagat
     3301 ttgacagcat tcctggcttt gaatgaagct tggtaatttt ggatacccct tgtctcgagt
     3361 tttgtttcaa aagaaaactc tgtcatgaaa acggcaagca gggccaaaat aaccagcgtt
     3421 atcaacaaag caattccacg ttgcggactt ttatctaagt gtgaaggtgg ggtcactaaa
     3481 aggtgagaca tgaattttta gatttgcagg ctgagattaa cacctcaacc cgcagtccaa
     3541 agttttcatt agcgtaaaat tttaaggccg ttgcttgaca ggaaatagaa gagctgtttt
     3601 tgctcacggc tacaatactg ctgatatttt atgaacaacc actggttgct ccacaggaag
     3661 cacatttgag gcatgaaccg ttacgtacca tagtgaactg ctgacaggtg tgacaggctt
     3721 caccctcata acccttcatt cgtgcttctt gaacaagggt tgttgtggaa cgttcctgtg
     3781 tctcccaact ttctgtcgat gtatcagtac caaaatctga attgccagta aattcagcat
     3841 gtacctgttc cggttttgga tcagaatttt ttaacagagc aaaatctact ccttcaaaat
     3901 cgctttcctc ctgttccaga aaggccagac tttcttcagt gctgagagtc ctctccgaaa
     3961 ctacttgttc gttgtcaaac tcgggttgat cctcattttg tgtatgtact gcactggctt
     4021 gtaactcgtc agcattgacc tgagccagat cgttgcgacc aaggtaagaa attgcaagtt
     4081 cacggaaaat gtaatcaatg atggatgtac tcattttgat tgagtcattt ccagaaacca
     4141 taccgtttgg ttcaaaacgt gtaaaaataa aggcttccac aaattcttcc agtggaacac
     4201 catgctgcaa gcccagtgaa attgcaattg caaagctgtt catcaagctt ctgaaagcgg
     4261 ctccctcttt gtgcatatca agaaaaattt cgcctattgt accatcttca tattcaccgg
     4321 tacgcagata gagtttgtgc cctccgatga ccgctttttg agtgtagccg ctgcgacggt
     4381 gtggtaattt tctgcgtttg gctatgtagc ggtgaattat tttttccgca atccggactt
     4441 gttccggttt ttcagcagca atatctcctt caacctcatc ttcgtcccac aagtactcct
     4501 ccacattcac attaagtggc tgactaagtt tggaaccgtc gcgatatagc gcgttgcact
     4561 tcaatccgta atgccaggaa gaggataaag cctctttaat atcagtgata ttaacttcat
     4621 ttggcatatt gatagttttt gagatggatc cggaaataaa aggctgggcc actgccatca
     4681 tgcggatgtg cgcttctgga gcaataaaac gtttaccttt tcgaccacat ttgtttgcgc
     4741 agtcaaaaat gctgtaatgt tccggtttga tgtgcggagc atcctcaatt gtcattgttc
     4801 cgcaaatata atcgtttgca gcctcaattt ctttactgct gaacccgaga tgcttgagaa
     4861 tactgaagtc aaaatctccc agttgctcgt cactcaatcc cagcgtttcc ttacaaaatg
     4921 actcaccaag agtatacatg ttaaaaacaa agctgatatc aaatgcagta ggcaattgct
     4981 cttcaatgcg ttttaggacc tcgctcgtaa atcctttggt acgcaaaatg tttgcattga
     5041 tgcaggggct tccgtccagt cgtgcgtgcc ctttgatata gtgtacaatg tcagaaactt
     5101 ccgagtcatc atatccaagt ttatataaag ccggtggaat cgaattgtta atgattttga
     5161 aataaccgcc gcctgcaagt ttcttgaact taaccaaagc aaagtccggc tcgattccgg
     5221 tagtatcaca atccatcagt aaaccgatag ttccagttgg tgcaatcaca gtgacctgtg
     5281 cgttacggaa accgtgtttt tctcccagtt ttagggccgt attggcatct tgttgagctg
     5341 ccttcagcag ttctggagga cagtgctgac tgtctattcc aaccggtttg atggtcaatc
     5401 cttcatattc actatccggt gcacgaaatg cagcccgccg atgattcctg atcacccgca
     5461 gcatattatc cttgttcttt tcgtagccgg aaaatgtacc aagctctttc gccatttcag
     5521 tagaagttgc gtaagaagtc atgtgcataa ttgaagtcac ggcaccacaa atagcaaggg
     5581 cttttgctga atcataagga ataccgttaa ccattaaata tgtccccata ttggcgtaac
     5641 ccaagccaag agtgcggaac tcgtaagaga gatctgcaat gtttctgctt ggaaattgtg
     5701 ccatcaaaac actaatttcc agaactattg tccaaattct tgaaccatga cgcaatttta
     5761 caatatcaag ttctcccgtt ttaggatctc gaaactgaag caaattcaac gaagctaaat
     5821 tgcaggctgt atcatccaaa aacatgtact cactgcatgg gttagatgcc cgaattggac
     5881 catcagccgg acatgtgtgc cactcgttga ttgttgagtc aaactgaatt ccaggatccg
     5941 cagaagccca ggctgcgtat gcaatttctt cccaaagttc ggtagccttt agttttttgc
     6001 aaggaaccgg ctctctgcct tctttttctg ctttgacctt ttcaactcgc cagtagagat
     6061 tccagtcttt gccctgttca actgcttgca tgaaagcatt attcaagcga acagaattat
     6121 tagagttttg cccgccaact gtttggtatg cttcagaatt ccaatcagta tcgtactcat
     6181 caaacaatac tccagtatat ccctgtttgg ataaatggat tatgcggtac acgtagctga
     6241 aaggcataga tgccaaacgg gaagctttga caacagagcg cagatctttg ttaagttcta
     6301 tatcaaactt ttcagactca ttctcccagc cgtgaattgc ttcaaacaac tctttgatca
     6361 gcctttttat cgttttggaa cctgaaacca gagcggcaac tttttgttcc tcgtgaactt
     6421 tccagttaat aaattgttca atatcaggat gatctgcatc cagacagatc attttagctg
     6481 cacgacgtgt tgtaccaccg gacttgatgg cacccgcagc tgaatctcca attttaagaa
     6541 atgacattag tccggaagaa acaccaccac cggaaagcgg ctctgcatca ccgcgcaaat
     6601 cagaaaaatt agaacctgtt cctgaaccat atttaaatag acgggcttca cgggtccaca
     6661 aatccattat gccgccatct cgtaccaaat cgtccttcac tgattggatg aaacatgcat
     6721 gtggttgtgg tcgtgtgtat gcatctttag aaggcattac ttcttctgtt tttggatcta
     6781 cgaaaaaatg tccttgtgct ggtccgctca aaccataggc ccagttaagt ccggtattga
     6841 accattgagg agagtttggt gcagccattt gattggccag catatgacat aattcgtcat
     6901 aaaatgccct cgcatcttct tctgtgtcga aatagttgtg tttgtaaccc caatacgtcc
     6961 agcaaccggc aagccgatga aaaacttctc tcgcatcggt ttccgaaaca gtagtatcaa
     7021 ctgctgaaga atctgcctct gaagcttcct gatcagcctt agcctggtca taaagagagg
     7081 gttgcagcca gtcaggaact cctttttctt ttgcggattt caagtgcttt ggtatgccgg
     7141 ctttgcgaaa atatttctgt gccaggatgt cagtggcgac ctgtgaccaa tgttttggta
     7201 cagacacatt atcccacttg gagactattg aaccgtccgg gttccggata acggaagcac
     7261 ggttttcaaa ttctattgat ccgtacggac tttcaccttc catggtgaac aggcgcttaa
     7321 ttttcataga tgccttttct tgatatgtat tctaaaataa gttttaagtt taaccaatcc
     7381 atggtgtatt ttaaaaatag tatactaaaa ctggtaaaaa ttgcaggttg aatttgtact
     7441 ttttttctgc tttgtttatg caaaaaacat gaaaattcta ttgccgtaga gccaaagcag
     7501 aaaaataagc aatcagttac atcataaagt tctttatttt gctggtttag tgtttctcaa
     7561 gttatagata aaatacaatt cccggtattc taagtgatat ataatgataa ttatatcaac
     7621 aatataaacg gataaaagct tgaatttact tgagaactgc aaccatatgt gagtacattt
     7681 gaaacaaagg ctgcatgaca aaaaatataa taaatatgca ggtatcataa tgtaatttat
     7741 ctggttcaga aaatcaataa aaaatatctt ctgttaaatc aaacataaac aattagttgt
     7801 ctgatatata aagtaaaata aattggattt atccgaaaca atgcaaaaaa aacgtagtcc
     7861 gattccagcg gtttatcttc atatcccttt ttgtcgcacg atttgtccgt tttgctcctt
     7921 tacggtacgt cgtgatcgag caaaaatgca tgaaaaatat attcggggta tggtaggcga
     7981 aattgaccga cgtgcagaaa tgctgaaaga catggaacgg aaagagaatg aagaaaaact
     8041 ttctgggcag attttgctca aatcaattta tatcggggga ggtacccctt cctgtctaac
     8101 tataccggaa gtgtcagtgt tacttacaaa agtccgtgaa aattttccct ggtctgatca
     8161 aatagagatt gcttttgaaa tgaatccgga tgatgtaaat ccggaatatc tcagtggact
     8221 tgcagatatt ggtgtcaatc ggctcagttt gggaggacag agtttccaga cttcaactct
     8281 tcagaagttg ggacgttgtc atactgttgc agatctacgt aatgcaattg cagtcataac
     8341 aaactcatca tttgataact ggaaccttga tctgatgttt ggtattcccg aacagagtat
     8401 tgttatgttc aaaaatgatg tcgaggaagc actgagatac gaaccttccc atatttcatt
     8461 gtatggcctt gaaattcatg aacggacgcc ttttggtaat aatcctcaaa tccggaactg
     8521 ggaacctgaa catctggaac aatttgagga aatgtatttg tgggcgacag acaatctgga
     8581 aaaggcagga ctgtttcagt atgaaatttc taacttttca aaaaaaacta acgaaggccg
     8641 acaaaatttg ctggtctggt ctggtcaaga atatttggga tttggagttg gagcacactc
     8701 ataccatagg caaacacgct ggggcaatgt tcgttctatc aggacttacc ttcagcatct
     8761 tggacaaaat acatggccaa ctgaatttat agaacaactt tcaggaaaac aactggcagt
     8821 tgaatttttg atgctcggtt tacgtcagtg tgaaggaatt aatattaaag aatggcagac
     8881 gcgctatgga ttgcatttgc aaaatcaaca gttaaatttt gttcaagaac tttgcgatga
     8941 tggacgtgca ttttgggaag gtcagcactt gtgtttgacg cccaaaggaa tgttgctggc
     9001 agacagaatc acagttcagc taatgccata agccggtgaa aattaactgc actaacaaaa
     9061 atttcataat tactttaaag ttttttttaa gaatgccgaa aagcaagaca agggttttat
     9121 ctgctagctg aaacattgtg ttagtttcaa cgcagaagcc taactttatt aattatggga
     9181 gaacaaaatg agaattcgaa gtaatgcaac atctttgaat acattacggc actcagacaa
     9241 caatttaaga aacgttagaa gctcaattga aaaattgagt tccgggacaa agataaacag
     9301 tgccgctgat ggtccggctt cgcttatagc ctcagaacga ttgagaggac aaattgcggg
     9361 attgaggcag gcttacagca acaatgaaaa tgctattgcc atgtttcaaa ctgccgaggg
     9421 ggccctgagt gaggccagca acatattgat tcgtttgaag caattatcag ttcacgctgc
     9481 aaacgaagca gttaacgatg attcaatgtt ggctgcagat cagcatgaag tggaaaactt
     9541 gcttagcact ttagatcgaa ttgtgaaaac agccgagttc aacggtagaa tattacttga
     9601 tggtagcatg ggtgcaaatg gtgcttctgt aggcaataac atacgtttcg ttaatgctga
     9661 aacttggact gaagcttccc cgatggaggg atatgaagtt gatattacac aggtagcaac
     9721 gcaaccatat ataagaggct ctgtaccgtt gacagtacaa aatataggtg aaggagttaa
     9781 aatattgctc agtgaaggtg gccgcaacgt tgaagtcgac acaaggtttg ggaaagttaa
     9841 agaaaatatt gaagaagttt tgtcaaacta taagcaagat ccttcacgct ttcccgcaga
     9901 ggaggcttcg aaaaatattc gagcgattgt tatcaattcc attaacgaag caatagaagg
     9961 aagtggttta gctttggagg cctttgaaac accagaaaac acattttata tccgacacaa
    10021 agattttggt gcttccccta ctttttcgct caccactaat atttcaggtt tgctgacaaa
    10081 agagtccaat gttgctgaat catcaatacc gggcttggat gtagccggaa aaattggtga
    10141 cagttttacg aatgggaaag gacaattcct gactgcaatc aaacgttctt ctcctgcaca
    10201 gggaatagcc atacaatatg atcgttctat tggactcaaa gaagtgcctg tcaaaaatga
    10261 gcaaggtgaa gtcgtaggaa cagaatttgt tgaagagacc caggaagaaa tcgtgggttc
    10321 tccaagtaat ccaataatag aaggctatgt tcacatatcg caacagacca agaatgttgg
    10381 tctcggaaca aaaccgggtg cggataaagg cttttcatta aaaaacattc gcactaataa
    10441 cttggcactg ggtgtggaaa atgaaagcgg ttttcgaagt ttatttgata tcgacttgac
    10501 cagtaaacaa ggtgctgata actcaggcag acttattgag aaggcaattg acgaaatatc
    10561 tgttttgcga ggagaaattg gttctttcca gaaaaacaca gttgaaagca atctaaattc
    10621 gctcagagtt gcagaagaaa acattagccg tggagagtca atcataagag acactgatat
    10681 ggccgcagag atgtcaaaat taactggtaa ccagattctt ctgtcggcca gtcaatccat
    10741 gcaggcacaa gcaaatcaat tacctcaaaa cgtgctgcag ctcctgcaac agcagtaatc
    10801 cgtaggcaat ggcgcatgga ggcgtcagcg aacgggtttg aaaaaagctg ttttaaatcc
    10861 ccaaatatcg atgctggatc tccttatctg caattagcgc cggagaatca ccagaccaga
    10921 caattttccc cttttccatg atgtaatgtc gattagctag gtgagtgaga gcttctatgt
    10981 ttttatccac gacaagaatc gactgacctg aagtttttaa gccattcagg cattcccaaa
    11041 tttccatttt aactaacgga gctaaacctt ctgtggcttc atccaaaatc aagaggcggg
    11101 gatttgtcat taaagctctg ccgatggcca gcatttgctg ttctccacct gaaagctggt
    11161 ttcccatgtt tttagcacgt tccagcagac tcggaaacag attgaagaca cgatccagtg
    11221 tccaaggatt cttgtttttg aaacgatttg ctgaagttgc gactaagttt tcaattacgt
    11281 tcaaattggg aaaaatttga cgcccttccg gtaccaatcc cattccggat tgagccacac
    11341 ggtgtgtagg ccattctcgt atttgactgc cttcaaattt gatttctccg gaacgagctc
    11401 taaccaaacc cataattgaa ttaattgtag tcgttttacc cattccatta cgtcccagta
    11461 aagttactac ttctccctca ttcacctgta gatccactcc aaacagcacc tggctttgtc
    11521 cataagaggt ttccaggtgt ttaagttcaa gcatgataac tctcctctcc aaggtaagct
    11581 gctatgactt ccggattttt ccggattgca tcaggagtat cagtcgcaat tcctctgcca
    11641 ttgaccagaa cagtgattct gtctgccagt gtgaatacaa catccatatc atgttcaatc
    11701 aacagaatcg taagtttatg tttcaattta ataagaatgt caaccattgc cagagattct
    11761 ttgggtccca ttcctgccat tggctcatct aacagtaaca ttttgggatt tgttgccaga
    11821 gccattgcaa tttccaactg acggtgttcc ccatgagaaa gcagtccggc tataaatcct
    11881 gcccgttttt ccaaaccgac catagctaaa aattcaattg caggtcgccg aagttcctgt
    11941 tcttgactgg cgtttttcca aaaatgaaag gaatgtcctg catgtgcctg aaccgcaagt
    12001 gcaacattat cccaagttgt catgtcatga aaaatattgg ttatctgaaa ggagcgggcc
    12061 aaaccgtgtg ccgaacgaag atgtaccggg actcccgaaa tattgtttcc atcaaacaaa
    12121 atgcttccgg aatcagattc aatttctcca gtcaattgac caatcagagt agtttttccc
    12181 gcaccatttg gacctataat agcatgtata tcccccttct ttacttgaaa cgagaggttg
    12241 tctgtagcta ataaagcacc aaaggatttg aaaagtgagt ctacttttaa cattttaatc
    12301 aacctcttca ttcttaacag gagagtttct gctccaccat tgatcaagtc ccacaagcat
    12361 tccgtctatt ccgccacgtg caaaaatgac aaccagaacc agcatgggac caaaaaataa
    12421 ttgccaatat tctgtgattc cagaaagaaa ttcctccaaa aaaaggaaag caactgcacc
    12481 aattacaggg ccaaaaagag ttcccaatcc ccccaaaact gtcatgatga tcaattctcc
    12541 ggaatgggtc caatgcataa cagacggatt aacaaattca gtctggtttg cagataaaac
    12601 acctgctatt ccacaaattg ttccggcaat tacgaaagcg acaagctgat atcgaaaaac
    12661 cgggaagcca atggcgcgca tacgttgttc atttgatttt gcaccacgaa tcaacattcc
    12721 aaaacgagag ttgattaacc tgtgtgaaag atacaaactg agcagcaaac agacaaagtt
    12781 caggtaatag agtgaattat catttgaaat atcaatcaaa ccattnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnagaca aagttcaggt aatagagtga attatcattt
    12961 gaaatatcaa tcaaaccatt aaagtcactg cgcatgtata gacttaaacc gtcatccccg
    13021 ccgtattttt cgttacctac tgccacgtaa tacaacatct gcgaaaaggc cagtgtaatc
    13081 ataatgaaat atactccacg agtccggagt gaaattgctc ctatcaccaa ccctgccagg
    13141 gctgaaaaca caattgcaag tgcaatttga aggaaaccat tagccatcca ttcaattcca
    13201 tcctcaacgg catgcatggt tccaattcca accatatatg aacctattcc caaataaact
    13261 gcatggccaa agctgaccat tcctccgaag cccaaaatta aattcagact gactgcacca
    13321 atcgaaagaa taagcatcct ggaaaacatt actgtgtaaa acggctcacc aaaatagtct
    13381 gcaagcagtg gaacaacacc taaaccaagc attaaaataa ctataaaaat tattcgtgga
    13441 gaccatttca tctaggcacc tcttggtgga aaaagtccct gaggtttgac ggccagaatt
    13501 agtgccatca agaggtagat caacatagag gaaatagcgg gtgctgcagt atcggccaca
    13561 tcattgggaa aaaatattcc taagatatcg ggaagaaagg agcggccgac agtatcaatc
    13621 aaacccacca acaaagcagc aacaaaggca cccttgatgg aaccgactcc cccaattaca
    13681 atcacaacta aagtaatgat taaaattcct tcacccattc caatttcaac agtcatgatt
    13741 ggtcctgcca ttaaacctgc taaaccagcc agcgcagctc ccagaccaaa aacaaaagta
    13801 tatagtaaat tgatgttaac tcccagtgca cctaccatct gccgattact tgctccagca
    13861 cgaatcaaca tgcccagttt tgtacgtgat acaagcaggt agagtaaaaa agcaaccagg
    13921 attccaactg caatgatgac cagccggaaa gcaggataac gaattcccag gaagagctct
    13981 acgctgccgt ttagtacttc tggaagtgga atgtctaaac cgattggtcc ccaaaggagt
    14041 ctgacaaatt cattgaaaaa aaggataagt ccgaatgtag ccaaaacttg atcgagatga
    14101 ttgcgggtgt aaagtgtgcg cagtgcaatc atttctacga tcatgccaaa agccatacag
    14161 gcaggaatag ccaaaatagc gcccaggaga aatgaacctg tccattttat gaaagttgct
    14221 gcgaaatagg cccccatcat gtagagcgac ccgtgtgcga ggttgatcag gtccatgatg
    14281 ccaaagatca gggtcaggcc tgctgccagt agaaatagta aaattccaaa ttgaaacccg
    14341 ttcagggttt gctccaaaag cagggtccag ttcaccagaa ccttttacta gtgttgtgac
    14401 agtgtggaag gaaagacgga gaggaggagg aacacaagta cctcctccaa ctggaaagaa
    14461 acaaactacc aggacatctt acaatctttt gcgtaaggat cctggtggtc ggtgtaaact
    14521 gtttttatga tagttgtggt gtaattccct tcagcatcct taattacttt tcgcaggtag
    14581 aaattctgaa ttgggaaatg gttatttcca tatttaaacg gaccgcgtac tgatggaaaa
    14641 ttagcttttc gcatggcagt ccgcattcca tctttgtttg acatattacc accaactgct
    14701 gttacagcac tgttaatcag catgatagtg tcataagact gtgctgcata gaaagtcgga
    14761 tagcgtccgg tcttttttcg ataatcggca acaaaacgtt tgttaaccgc attatccaag
    14821 tctggagccc agaactgtgt catcaaactt ccctcagcca aatccttcaa acgaggaagg
    14881 ctcaatgagt caacagtaaa agctgagtag agtgggattg ttcctttcaa tcccgcttga
    14941 gaaaattgtt tgaaaaactg tattccatgc tttcctggat aaaaaacgaa cactgcatct
    15001 ggtttagctg cacgtatttt tgccagttct gcagaaaaat caagttgtgc cggaaacttg
    15061 gtcatgtctt tcccaacgat attaccttta aaagttcggc ttacaccagc aaccattcct
    15121 ttgcctgcag cataatttgg tgccatgatg taaaggttct tgataccaag ctggtttaat
    15181 acctccccca tagccattgt ggtttggtca ttctgccagg acgttgagaa aaaatcttcg
    15241 tggcagagtt tgcctgccac tggacccggt cctgcattgg aggtaatcat aaaaggtcct
    15301 tttcctacta ccgaattccg ggaagccatt aatacgtggc tccaaatgta acctgtgaca
    15361 aaatgaactt tatctttttt aactaatttt tctgttttct gtttaccgac agccggcttg
    15421 aaagtgtcgt cttcataaaa cattttaaca tctgaatttc ccattttacg acccaaatgg
    15481 tccagtgcca gttctactgc gtcccgcata tcattcccaa ttgcacctgc acctcctgtg
    15541 agtgtggtca caaatccaat tttaactgtt tcagccaacg caacctgacc aaccagcagt
    15601 acaccaatag acagtgtccc caaaataccc agcctcatct tacctcctaa atttaggtta
    15661 aaaaattttg ttattcaaga aagtcttgaa ttaacgaaaa gtaaaagaaa agattctaaa
    15721 gaaaaatgta tcaagtgtca acaaaaaaca caggaattct ctgtttctgg atgatacaga
    15781 aataacagct gtttgtttgt taagagtaaa ttttcagtaa aatccagcaa attggaaaag
    15841 aaaatttgtt tttcgacgtt aaattttgaa cggacttgag acacggttga ctacaaacaa
    15901 tgtatgacag tggccataat tatgatataa gtcgaaaact cgttactgcc aattacagca
    15961 agagatctgc ccttaattta agcggtttat tctgtaagtc ctaccagtat tccaggacgc
    16021 aactggtgtc gcgttcaacc aggttgaaaa attttaagtt atgacccagt ccaggactgc
    16081 tccggtgtcg caactcatgg atcagggtgt ttaataattg tgtttttttg ggccagccaa
    16141 aaaaccagaa atttgaacgg tgaatcttga tttgctcttc cccccagtat attcctgctt
    16201 ctccatactg aatccaattt ctatgcaccg gtcgctccct ggtgtagcga atcggtactg
    16261 tcctaatttt gactttcatt attgatggaa aacatggaac ttccagaagg cgctctgcat
    16321 atgctgtaat cagttcctgt cttgccgcag gattttcgag aagaggatca agcagatgcc
    16381 agcggtccag gacaaaaaat aaggtactac caccaagcag aagggctaaa aaccattgtg
    16441 catatttgga tagtggagtt tttcttgagg aacttttgtc aaggtgtctt tttttccagc
    16501 gaaaccattc ctcccattca agttgcaaat tattaatttg ctcagtttgc cattgttcgg
    16561 aaatatcttc aggagatttt atattattgg gcagttccgg attctgaaaa aataaatgct
    16621 cctgacattt gtgacagtga atccggacaa agcgctcacc gtttttaccg gacattggta
    16681 caaataagaa tttttcttca ccacaagaag gacaatggag agtaacatct tcttcaggag
    16741 aagtaacagc agatggcatt gaaaaatcag gttatttaca gtaaaacatg cattagttat
    16801 ccttgagtgt ctgcgaggca ataattagtt tttgaacctc agaagcacct tcatagatcc
    16861 tgagcgcacg gatttcccga tagagttgct caacagcgaa tccggaaacc acaccggatt
    16921 caccaaaaat ttttacagct tcgtctataa cacgttggtg ccgcttctgt tgcaaataat
    16981 ttagccatgg cagcttcacg tgtaatcctg tctgcactat tgtctttggt ccaggcagca
    17041 cgataaatga gaagtgcact tgcatcaata tcaagtgaca tgtccccaag tttggcttgg
    17101 atgagctgta aattactcat tgga
//
LOCUS       DTVD01000243            4763 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6161_length_4763_cov_403.995752, whole genome
            shotgun sequence.
ACCESSION   DTVD01000243 DTVD01000000
VERSION     DTVD01000243.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_4763_cov_403.995752"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            145..1689
                     /locus_tag="EYO68_09000"
     CDS             145..1689
                     /locus_tag="EYO68_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIC07974.1"
                     /translation="MIAKTFSATVLGIDAYLIEVEVDVSVGMSVFNIVGLPDSTIKES
                     RDRILSAVNNSGYSFPVRRVVVNLAPAPLRKIGSGFDLPIALALLGTMGLFPPENLSR
                     AMVVGELSLDGEVRPVRGILPVAVATLEHELEQLILPKANEAEAAVVEGIRRVPVSTL
                     SEVVQYLKGDLQITPLDYDPAEIFSQQQHFEEDFQDVKGQEHVKRALEVAAAGSHNLL
                     MLGPPGSGKTMLARRMSTILPRLSFDEALECTKIHSIAGMLSSGTSLIAQRPFRFPHH
                     TISQAGLVGGGGIPGPGEISLSHNGVLFLDEFPEFPRATLELLRQPLEDGKLTISRAA
                     MSLEFPCDFMLLASMNPCPCGYHGAPSGQGENRRECNCAPQLIQKYRAKISGPLLDRI
                     DIHLTVPAVAYEKLAEARQGESSKNIRERVVRARQVQEQRFRNSSSRNNSGMNRKELE
                     NHAELADSSRKILQLAMTKMGLSARAYDRILKVARTIADLAGEQKIDTPHVAEAIQYR
                     NLDRPV"
     gene            1842..3578
                     /locus_tag="EYO68_09005"
     CDS             1842..3578
                     /locus_tag="EYO68_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIC07975.1"
                     /translation="MNNTFPFTAIVGLELAKRSLLFHAVDPRLGGLLLMGHRGCAKST
                     LARAFREILPAPIYSEMSPFVEVPLGTSEDRLLGSIDASSLLENGKWSAQSGLIEQAN
                     GGVLYIDEVNLLPDHLVDSILDSAASGQHRIERDGLSQTVNARYILIGSMNPEEGDLR
                     PQLTDRFMHGILIHDDFSVEERREIVRVRMDFDDDPQNFFAEQQTKLEQLRSQILGVR
                     QELKNVDISENLRTEVAQKAARLKLEGVRAELGVLRTARCAAAWRGDTTISEADLAEA
                     WILCLGHRRTDQPPESQNSKPKIKLPKPPADDLESKRLKMPKTSFSPEEANAEEISLE
                     ASHNLAHSELASWWKSTPKKQLSDNFVSGTSRPVPTHVSHSRLCWTSSLKASLMRGWT
                     PGNPWHLRFRKPARRSNLWLFIDASRSTGTFGSRTSAHFLAAARNVIQTLGAKTFGSR
                     FHVLVLQNGKIGWWIKRGTAQAVQKTLGRLTEASGKSHLTPALNQLRNAIQKQGVLEG
                     DRVLLCSDGMLSPETEKTVSESKKRFRKALLNLSQRISTTAWLHPQLQRGMRHWLPEL
                     TEGTPVRLIALD"
     gene            3607..>4763
                     /locus_tag="EYO68_09010"
     CDS             3607..>4763
                     /locus_tag="EYO68_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HIC07976.1"
                     /translation="MGLIVAGMHSSGGKTAVTSLLLAALRKRNFIVQPFKVGPDYIDP
                     GFHFHYSKQHSINLDPWIMGREHILQAAKKFTENAFGIAEGVMGLFDGSDPTNDSGST
                     MEVARRLSWPILLVVPCQNSGRSITAAIQGFVAEAGGPEHFAGIILNQVNSESHADYL
                     SKACASLQIPILGALPEIPELRWPERHLGLQPGVEQKLPEADHLAELAEKYFDLKLLI
                     KKFPVLSASAAPVKNLQSTTPKFSKRIAVAQDEAFHFYYVANLEWLRQQGADIISFSP
                     LHDNKVPENVDGLILGGGFPEVFAEEMSANRSMLISLKKSVESGIPCYAECGGLMLLA
                     DGLKVHSGKCYSMAGVVPGTVEMTKQLQNFGYCKIDSLKSGEIRGHEFHYSR"
BASE COUNT         1282 a         1070 c         1147 g         1264 t
ORIGIN      
        1 attcctctta ttttctctca taaaaacttg cactcacagg taagtctttc taccatcaat
       61 cctatattta aagaaaacgc attgtcattg atgggttctg atcatgactg tattttcaac
      121 actccctttt gaaatagagg caaagtgatt gccaagacat tttctgctac agtcttagga
      181 attgatgcct atttaattga ggtagaggtt gatgtttcag ttggaatgag tgttttcaat
      241 attgtgggat tgccggacag tacaattaag gaaagccggg atcggatcct gtctgcagtc
      301 aacaattcag gatacagttt ccctgtccgg agagtggtgg taaatcttgc accggctcct
      361 ttgcgtaaga ttggttccgg ttttgattta ccgattgctc tcgctttgct gggcacaatg
      421 gggctgtttc caccggaaaa cctgagccgt gcaatggtcg tgggagaatt gtcactcgat
      481 ggtgaagtcc gtcctgtacg tgggattctg ccggttgcag tggctaccct tgagcatgaa
      541 ttagagcagt tgattcttcc aaaagctaat gaagcggaag cagcggttgt tgaaggcata
      601 agaagagttc cggtttccac actgtctgaa gttgtacagt atttaaaagg tgatttgcaa
      661 atcacacctc ttgattatga tccagcagaa atcttttcac agcaacagca ttttgaagag
      721 gattttcagg atgtaaaagg tcaggaacac gttaagcggg ctcttgaagt ggcagctgcc
      781 ggttcgcata atttgctgat gcttggtcct cccggttccg gcaaaacgat gcttgcccgg
      841 cgcatgagta ctattttgcc gcgactcagt tttgacgaag cactggaatg cacaaaaatt
      901 cacagcatcg ccggaatgct ttcatccgga acttctttga ttgcacaacg cccttttcgc
      961 tttccgcatc ataccatatc gcaggcaggt ctggttggcg gaggtggtat tcccgggcct
     1021 ggagagattt cgctttcaca caatggagtc ttgtttctgg atgaatttcc ggaattccca
     1081 cgggcaactc ttgaattatt acgtcaacct ctggaagacg gtaagctgac tatttccagg
     1141 gccgcaatgt ctttggaatt cccttgcgat ttcatgttgt tagcctcaat gaatccctgt
     1201 ccctgcggtt atcatggtgc accttccgga cagggagaaa accgccgtga gtgcaattgc
     1261 gcaccgcagc taattcaaaa ataccgtgcc aaaatttccg gccctttact cgatcgaatt
     1321 gatatacact tgacggtgcc tgctgtggca tatgaaaaac tggcagaagc acggcagggc
     1381 gagtcttcca aaaatatcag ggaacgagtg gtccgtgccc gtcaagttca ggaacagcgc
     1441 ttccggaatt cttcaagcag aaataattcc ggaatgaacc gcaaggagtt ggaaaatcac
     1501 gcagagcttg cagattcctc cagaaaaatt cttcagctgg caatgaccaa aatgggtttg
     1561 agcgcccggg catacgatcg gattctcaaa gttgcccgga ctattgcaga cctggcaggt
     1621 gagcagaaaa ttgatacgcc tcatgttgca gaggccattc agtatcgaaa tctggatcgt
     1681 ccggtttagg tgtcggccag aaatttgact gttcctttat ctaaacagca ttgatcgcaa
     1741 attaatataa aaaccaaaac gtatttttgc tgaaacgaac ttgagtgttt ttctgcttct
     1801 tggcagaatt aacaactttc agaaaataaa cagcataaat catgaataat actttccctt
     1861 ttactgcaat cgttgggctg gaactggcca aacggtcctt gcttttccat gcagttgatc
     1921 cgcggctggg cggcttgttg ctgatgggac atcgaggctg cgcaaaaagc actcttgcac
     1981 gggcttttcg tgaaatttta ccggctccaa tatattccga aatgtcaccg tttgtggaag
     2041 tacctcttgg aacatcagaa gaccgtctgc tgggctcaat agatgcgtcc agtttgcttg
     2101 aaaatggaaa atggtctgca caaagcggtt tgatagaaca ggcaaatggt ggggtgcttt
     2161 acatcgatga agtcaatttg ctgccggatc acctggttga ttcgattttg gattctgcag
     2221 ccagcggaca acaccgaatt gagcgggacg gtctttctca aactgtcaat gcccgctata
     2281 ttttgattgg ctcaatgaat ccggaagaag gcgatttgcg gccgcaattg acagaccgtt
     2341 tcatgcatgg aatattgatc catgacgatt ttagtgttga ggagcggcgg gaaattgtaa
     2401 gggtgcgaat ggattttgac gatgatccgc aaaatttttt tgccgaacag caaacaaaac
     2461 tggaacaact gaggtctcaa attctgggag ttcggcaaga attgaaaaat gtggatattt
     2521 ctgaaaatct gcgcacagaa gttgctcaaa aagcagcaag actaaaactg gaaggagtcc
     2581 gggcagaact tggagttctc agaactgcgc gttgtgcggc agcctggcgc ggagacacaa
     2641 ccatttctga agcagatctg gctgaagcct ggatcctgtg tcttggacac cgccggaccg
     2701 atcaaccacc ggaatctcaa aactcaaaac cgaagattaa actcccaaaa cctcctgctg
     2761 acgatttgga gtcaaaacgc ctgaaaatgc ctaaaacatc cttctctcca gaagaggcaa
     2821 atgccgaaga aatttcttta gaggcatcac ataacctggc acatagtgag ttggcttcct
     2881 ggtggaaaag caccccaaaa aaacagcttt ccgataattt tgtttccgga acatcaagac
     2941 cggttccgac tcatgtttct cattcccggc tttgctggac ctcttctctc aaggcctcgc
     3001 tgatgagagg ctggacacct gggaatccat ggcacttgcg gttccgaaaa cctgcacgac
     3061 gctctaattt atggttgttt attgacgcaa gccgttcaac aggaacattc ggcagccgca
     3121 cttccgcaca ttttttagca gcagcacgca acgttatcca aacgcttgga gcaaaaactt
     3181 tcggcagccg ctttcatgtt ttggtacttc aaaatggtaa aatcggctgg tggatcaaac
     3241 gcggaacagc ccaggcagtt cagaaaactt tgggacggct tactgaagct tccggaaaaa
     3301 gccacttgac gcctgcactg aatcaattac gtaatgccat ccaaaaacaa ggtgttttag
     3361 aaggtgaccg ggtattgctc tgttctgacg gcatgctcag tcccgaaact gaaaaaactg
     3421 tttccgaaag taaaaaacga ttccggaaag cacttttgaa tctttctcaa agaatttcta
     3481 cgactgcctg gctgcatcct cagttacagc gtggaatgcg gcactggctt ccggaactga
     3541 cagagggtac tccggtccgt ttgattgcac tggattaaaa tgcaatcccc gtcaaaaaag
     3601 aaacatatgg gcctcattgt tgccggaatg cacagcagcg gcggaaaaac cgctgtgacg
     3661 tcattgctcc tggctgcact gcgaaaacga aatttcatag tccaaccctt caaagttggt
     3721 ccggactaca tagatcctgg cttccatttc cattattcca aacaacattc tataaatctg
     3781 gacccctgga tcatgggtcg tgaacatatt cttcaagctg caaagaaatt cacggaaaat
     3841 gcttttggaa ttgcagaagg cgtgatgggg ctttttgacg gctcagatcc aacaaatgat
     3901 tccggaagca ctatggaagt cgcacgtcgg ctcagctggc cgattttgct ggttgtacca
     3961 tgtcaaaatt ccggacgttc aatcactgct gcgattcagg gctttgtcgc cgaagccggc
     4021 ggtccggaac attttgcagg aatcatcctc aatcaggtaa acagtgaaag tcatgcagat
     4081 tatctaagca aagcctgcgc atctctccag attccgattt tgggagcttt accagaaatt
     4141 ccggaactgc gctggccgga acgtcatttg ggcctgcagc ccggagtgga acaaaagctt
     4201 ccggaagcag atcatttagc ggaattggcg gaaaaatatt ttgacctcaa acttcttatc
     4261 aaaaaatttc ccgtgctttc tgcgtcagcg gctccggtaa aaaatctgca aagcacaacg
     4321 ccaaaattca gcaaacgaat tgctgtggcc caggatgagg cgtttcattt ttactatgta
     4381 gcaaacttgg aatggctccg gcaacaaggt gctgatatca tttcgttttc accattgcac
     4441 gataataaag ttccggaaaa tgtggacgga ttgattttag gcggcggctt tccggaagtc
     4501 tttgcagagg agatgtcggc taacaggagc atgctgattt ctctcaaaaa atctgttgag
     4561 tccggaatac cctgttatgc ggaatgtgga ggtttaatgc ttttggcaga tggcttgaaa
     4621 gtacattctg gaaaatgtta ttcgatggct ggagttgttc cgggtacagt tgaaatgaca
     4681 aagcagcttc agaattttgg ttattgtaaa atagattcac tgaaatcagg cgaaatccgc
     4741 ggtcatgaat tccattattc gcg
//
LOCUS       DTVD01000244           17088 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_619_length_17088_cov_334.663712, whole genome
            shotgun sequence.
ACCESSION   DTVD01000244 DTVD01000000
VERSION     DTVD01000244.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17088)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_619_length_17088_cov_334.663712"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(1..90)
                     /locus_tag="EYO68_09015"
     tRNA            complement(1..90)
                     /locus_tag="EYO68_09015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:tga)
     gene            complement(437..1576)
                     /locus_tag="EYO68_09020"
     CDS             complement(437..1576)
                     /locus_tag="EYO68_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC07977.1"
                     /translation="MKILIGSNVHWWNAEAAYAAAIAQLLKQAGHSVFVLTRPDSLNS
                     KHLSERGLRLLTHINLNSKNPFQLLLAYRKLKKFLLEEQIDLINPHRSEGFPLFVFAA
                     RSLSTNRIENPIPVIRTRGTTRPLLQHWLNRKMHIDWTANYITAGEIVAERLLDAVPI
                     SPEKLKTIYYPVECSDLPLKPPGDYRTEFEIPQKAKVLAVVGRIRPVKGQRILLQSLS
                     QLLTEFPDLVLLIPYRDTASNEPEMQALHNDIRRFKLEAHVRLITVREDIRQLMGFAD
                     VGVVSSVESEVICRVAVEFFSVATPVVAFPTGCLPEIIRHGENGYLAETQTAESLTEE
                     LRKILANPELCERLGQGARRDAETRFNPQIMLTETLKVFEQALNH"
     gene            complement(1707..3014)
                     /locus_tag="EYO68_09025"
     CDS             complement(1707..3014)
                     /locus_tag="EYO68_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07978.1"
                     /translation="MAEEETEVTVDEDVPENFAAQIARDLMVIFQKQMDLDTASAEAA
                     AYIWKNTGTTGKVGYFIDATEMWLETQSAGDKYAALSWLAIANQSANNEDYDTFLHMM
                     INSIIKGYYNLEKPDIEYKGKKYSTYTSIVSNIFIRMLELNPTNGEIASNIFSIFIRN
                     EMELSAKSTAEEKETGSSIIPTDMQDLYDDVISYISDRGIFKPSPMSGTEENPNEHIQ
                     NLCERLRSTRRYVMQEVINERALEKRKQLELDLKNQLASAEEIVLVAPQFTDGLLLFV
                     QEKRYNFKYLSVEKVRMTLQLLGSITGAVYFLLGFMGYLGVHWVDGFVVCLVMLGLVR
                     ILLSRKQLKLFYPTDISKELEESSTAFINVMRNMSQEQMEHFMVRQIKLEHNQKYLTM
                     VPEYVKYLYAIMPDRKSMMISVDELSELVENSEIEVAKQLRGQ"
     gene            complement(3045..4823)
                     /locus_tag="EYO68_09030"
     CDS             complement(3045..4823)
                     /locus_tag="EYO68_09030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIC07979.1"
                     /translation="MKKTDINSDLYKIRHSLAHVLAQAVLEIRPDAKLGFGPPIDSGF
                     YYDFDLTEPLSPEDLPMLEKRMRQIIKKGQVFEREELGHDEMVERLDKDNQGYKIEQV
                     EDLSAQNEILSLYRNGPFWDLCEGPHVDSTRDIPKNCFSLDSLAGAYWKGSEENNMMQ
                     RVYGLAFETGDELKEFKEKRQLAMERDHRKLNSKQHYYTISEEVGKGLPLWLPNGTVI
                     REELEKLAKEKEFLAGYKRVSTPHITREELFHISGHLPFYEDSMFPPMEMDGERLFLK
                     PMNCPHHHMIYKAEPRSYRDLPLRLAEYGTCYRYEQSGELAGLLRVRGMTMNDAHIYC
                     SPEQAKAELLNVLNLHTQYYELFGLKDFWMRLSLAEKDTGKFVDEPELWQKAETLITE
                     VMEEVGVPYEAVRGEAAFYGPKIDFQVTNVIGREETASTNQLDLVMGKRFGLTYIASD
                     NQEHTPIIIHRAPLGTHERFVAFLIEHYGGAFPTWLAPLQVRVIPVASAFNDYAHGLN
                     DSLYSSFVRTELDDSHDSFSKKIRSAAVAKIPNILIVGENEQAGQTVTWQRYGSKDRK
                     TLAFDAFLELLQEEILKRQDWRTDDQ"
     gene            complement(4883..7291)
                     /gene="lon"
                     /locus_tag="EYO68_09035"
     CDS             complement(4883..7291)
                     /gene="lon"
                     /locus_tag="EYO68_09035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIC07980.1"
                     /translation="MSVQLKPVIVPLLPMREEIVFPGATVPFFVGRQASMDALERALA
                     GDRAIFVVTQRDSSVENPDENDLYKFGVLGRVLQVMRLPNGTVKALFEGSRRGKLLAT
                     KLDEKEFLAQVQPVDENAFDIDLEKLKKQAEMVRELFKNHVDQRKIKLSKEILDSLDQ
                     VDFLQVSDKVSSLLEIPGDKKQQLLETLNPETRLQQVLDILKDEMKIVQLEDKLKKEA
                     TDQISDTKKEDFLQDQLRNIQKELGQMDDPKAEMDVLDKQIIDAKMPEEVESEAKKEL
                     KKLRMMSPMSSEANVVRNYLEWLVSMPWQVRTEDNLDLKQAEKILDEDHYGLEKVKER
                     IVEYLAVASLKGKLKGPILCLTGPPGVGKTSLAKSVARALGRNFVRISLGGVRDEAEI
                     RGHRRTYIGAMPGKIIQSIKKAKSTNPVLLIDEIDKLYQSNISDPSAAMLEVLDPEQN
                     NTFVDHYLDVEYSLADVLFICTANSVQNITGPLLDRMEVIHLSGYTELEKQHIATQFL
                     TPRQRKFHGLDEKQLRFRKSAIEEITQGYTREAGVRNLEREIGKICRKIVTRLVRTKE
                     KKTTIVTPKLVHDLLGVPQFHHDQREERNEVGVSMGLGVTPMGGELLLIEVGLMSGSG
                     KVVLTGTLGDVMQESAKAAFSYVRSNAEYLGIDPEIFSKTDLHVHLPEGATPKDGPSA
                     GLPVMMAIISSFTKISVRYDVAMTGEITLRGHVTEIGGLKEKLIAAKRGLIETVLIPE
                     ENEKDLVEIPDEIKKGLKIKAVKNVKESLGLALEAHPNFVKDQQKIVPDISWNDTAEV
                     QHPA"
     gene            complement(7400..>8250)
                     /locus_tag="EYO68_09040"
     CDS             complement(7400..>8250)
                     /locus_tag="EYO68_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07981.1"
                     /translation="STVFALIADLTRPEVRTRANAAMGISIGIAFAVAFGAAPFLGEW
                     IGLDGIFWMIALLSAVSLVLVLTVVPNPETTQQLPEHTSFWSMFKIVWKVPSLRTISW
                     GGFVCGAGLSSTFFLIPMILVQHGFERAEMWKIYLPMMLAGVVAMVLAAIFAEVKNRF
                     REVMLFGIVLLLTSLVFMGLGQEQNRLIWFVAALYFFFMGFNVFEPIFPSLVTRSTTS
                     ETKGTAMGVYNFAQFIGHFVGATVAGALYVNNYLIFLLLLAVAEIWFFYLTLSFQNPE
                     KKIKTK"
     assembly_gap    8251..8350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8351..8766)
                     /locus_tag="EYO68_09045"
     CDS             complement(<8351..8766)
                     /locus_tag="EYO68_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC07982.1"
                     /translation="MSESAKTPIFTLSEKRSIYSLSGVLFFRMFSLFLLLPVFSVLAM
                     DLEGATPFLIGVAFGAYGLTQGFLQLPFGMWSDRAGRKLVIVIGLGLFIAGNFLAAFV
                     DSIHWMIVARFLQGTGAISSTVFALIADLTRPEVRTR"
     gene            9222..9437
                     /locus_tag="EYO68_09050"
     CDS             9222..9437
                     /locus_tag="EYO68_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07983.1"
                     /translation="MIDENSSTVIVNIHGLLGEQDCIQMDFEEELLVEEEQFIIDNVA
                     YEIVRVIKEDVEYPVVYVVILDILNHT"
     gene            9471..13016
                     /gene="mfd"
                     /locus_tag="EYO68_09055"
     CDS             9471..13016
                     /gene="mfd"
                     /locus_tag="EYO68_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIC07984.1"
                     /translation="MPATLQKILQKIKTDSTVVELQGLSGSSKALVVSMLSQIPEQPA
                     EKIIPLVVVCESFDVAEVLLNDLYYFFGKEGVHFFPFWDVLPFDNFSPHKGLVAQRFQ
                     TLDALLNSEVRVLITTPNGMMQRFLPRAAFQKNTLSLSTDFVGGKQELRQQLLNSGYI
                     QVDVVEDQGEFSAHGDIMDVFPLNQEKPVRMEFSENSELLYLKPFEIQTQRTAEAELT
                     SLKILPGSEILFNQETIYFARQTLPSYRKECTPEVLRRLKESLQKCESFPGIESLSPL
                     FYPKLETLFDYFPAEYLLVVDEENHITERAEHFYQEVFMEYELSKQQNKLTLSPEALF
                     LTHRELESRLKESAQVYLKSKVPGKKSERTIYQLQFSDNQSLRPGFEHSKATSAAGHM
                     VQLLQDWSKSGIPIILSAKNQTHADHFQQLLEDLGVESTVAGKQQVAKDCPWPKWLES
                     NTVDGLKEKIPILCGNVSSGFRRLDADGQTQFILLTQEEVFGEKKRSRRLQRTQVQQV
                     AGNLDDLREGDHVVHLDYGIGRYHGLQKITAGGSNNEFMLLIYAREEKVYVPVAKFHL
                     VQKYVNADGTAPKLSKLGEKAWKKTRTKVARAVDDIAEELAQIYAERKARKGFAFAPD
                     DHEMREFELRFPFEETPDQEEVIRSVKEDMETKLPMDRLVCGDVGFGKTEIAMRGAFK
                     AAQQEKQVAILVPTTILAQQHYETFVKRFEDTPFIIEVISRFRTDAEQKIILKRLLEG
                     KIDIIIGTHRLLSTDVKFNDLGLLVVDEEQRFGVKHKEKIKRFRAAVDVLTLSATPIP
                     RTLHMSLMGIRDLSLVNTPPADRRAIRTRLLPVNDYIIQESVSREIRRGGQVFVVHNR
                     VETIYEYGRYLESILPNIRIAIAHGQMREQHLEKVMLDYIEGRFDVLLSTTIIESGLD
                     IPRANTIIINNAQNFGLSQLYQLRGRVGRSDVQAYAYLLVPAEKILSGVAHERIQVLQ
                     DLNDLGAGFKVASRDLEIRGAGNLLGSEQSGQIASVGLELYTQMVDRAVKKLLQTESG
                     LAPEDIQVRLDHIEQSIPESYIHSSSHRLSLYKALGTLPTKEELWEFRNGVEDRFGIL
                     PESVLNIFRNAEVRLWGQLHGVEKIEHEQNRLRIQVKDASRLNHEKLVGWLCEQETPL
                     SYIPENTLDYKNVPPEMNAILKGLKNLEQVFNVYG"
     gene            complement(13533..14609)
                     /locus_tag="EYO68_09060"
     CDS             complement(13533..14609)
                     /locus_tag="EYO68_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HIC07985.1"
                     /translation="MSDSNLQQSIKVRDTISRMSFYDPPLENRNPEEHLLMDFNEPPL
                     PPPKEVIDKIAHFLKKRVHAYPVYGDFLEVLSKYTGASQDSLLLTNGSDQAIDVILRC
                     LLESGEDVAMVQPGFAMFKQVAGTIGCQVVGPLFPPDLHFPHEELKDAVNPQTRLIVV
                     VNPNNPTGTSVSQDQIEDLLKTFPDVPVLVDEAYFEFTGQTCVPLLPNYPNLIIIRTF
                     SKALGLPSLRLGYVIARPDFIVHLKKIRGPYDVNLVAVLAAQEQLNHPEHWQGVVRHL
                     MDESKPALEKYFDEQQVKYYSGAANFMLVEPHNIVKATTFLKENKILVRMMSAPISHT
                     FRMSLRMMPDMRRFMEVYSRFLNS"
     gene            complement(14616..14891)
                     /locus_tag="EYO68_09065"
     CDS             complement(14616..14891)
                     /locus_tag="EYO68_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIC07986.1"
                     /translation="MVTALVLLNIERQQIKLISQQLVYMQEVSEVNSVAGRYDLVAIV
                     LVINNQQIADTITVKFGELNGITATETLIAFNTFSRHDLEHMFSIGE"
     gene            complement(15349..>17088)
                     /locus_tag="EYO68_09070"
     CDS             complement(15349..>17088)
                     /locus_tag="EYO68_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07987.1"
                     /translation="QSSVDPDATTPAVVKMLLAPPNEHKLHVYVNDEKFIYWPQDKPI
                     FFWLGTSSDEKAELFPLLHSGTTNSAGHWKPDAANELEKYRSDGLKLEISSNQFVRWL
                     HHYNKKETTMRFIADGVAPESQLQFVSKKRLMDIRGLFFSGDAIATLSATDAFSGVEQ
                     TYLSVNGKTFLPSDGMKFSEEGYYLLRHYAVDRVGNTSETLQTRFYIDKTPPVTNLKM
                     VNATKNIFGPKSTLSIPSQDALSGVYLTYYNWNSGVFKRSYKNRILFEGVKEGANILQ
                     FYTEDLVGNTEGIHKVTLGFDKNGPEVSHEIKGDQYIEGGKVYLSSRSQIFLKAVDEM
                     VDIQSIEYQVNGLSYGNYGISFRPPVQTGEFKMDYQGIDIIGNKSILASANFVVDSQS
                     PTTRLITDGVTYRRGPNVIWVNTKTRISLKALDNVSGVQSIFYKFNENKELLYKGPFT
                     ISDEGRHTFQYSSVDHVNNKEVLTPVLLVVDDTPPEIKAVFNRSAVGSEGGDGEDKID
                     VYPLYTTLFLNADDNSAKLKGIRFSINGSPKEAYQEALLLDKPGNYYVIVEAEDNLGN
                     ISNKKMIFIVKAG"
BASE COUNT         5003 a         3687 c         3291 g         5007 t
ORIGIN      
        1 tggcggaggg agagggattc gaacccccga agaccttgca gccttaacgg atttcaagtc
       61 cgtcggtttc aaccactcac ccatccctcc gtcactaatt atactctata atatacagta
      121 attactcaag tctgaattct ttatctgtgg agtttttcaa aataattata gtttaataac
      181 aagtgtacta cttgtcatac cgaacttgat acgggatccg gattccacat catgctgaat
      241 tcaagaattg aaaggatccc ccctggaatc cgggttttag ctacgctaca cggatgtgct
      301 tcttcccgga caccccttgc aattgacatc ttgaatcgaa taatttctta atgatatttt
      361 tggataagat tatttttcag cagatactaa ttaattcctg ggccagtaca gcttgctgat
      421 cttaaaactg ttaatattaa tgattgaggg cttgctcaaa aactttaagg gtttcagtga
      481 gcatgatttg gggattgaat cgagtttctg catcacgacg tgctccctga ccaagtcgtt
      541 cacatagttc cggatttgcc agaattttgc gtaactcttc cgtcagcgat tccgcagttt
      601 gtgtttcagc caaatatcca ttttctccgt gccggattat ttccggaaga catccagtcg
      661 ggaatgcaac taccggtgta gccactgaaa agaattccac cgcaaccctg cagatgactt
      721 cagattccac tgaacttaca accccgacat ctgcaaatcc cattaactgt ctgatatcct
      781 ctctcactgt aattagcctt acatgtgctt caagtttaaa tctgcggata tcattatgga
      841 gagcctgcat ttccggctca ttgctggctg tgtcccggta cggtataagc aacaccaaat
      901 caggaaattc agtcagtaat tgactcaaac tttgaagaag gatacgctgc cccttgacag
      961 gacgaatacg tccaacaact gccagaactt tggctttttg gggaatttca aattcagtcc
     1021 ggtaatctcc tggcggcttc agaggtaagt ccgaacattc aaccggatag taaattgttt
     1081 tcagcttttc cggtgaaatt ggaactgcat caagtaatcg ctctgcaact atttctccgg
     1141 cagtaatgta atttgcagtc caatcaatgt gcatttttcg attcagccaa tgctgaagca
     1201 atggcctggt agtgccgcgt gttctgataa ccggaatggg attttcaata cgattagtag
     1261 acagggaacg tgcagcaaaa acaaatagag ggaaaccttc cgagcgatgt ggattgatca
     1321 aatcaatctg ttcttccagt agaaactttt tgagtttcct gtatgccagt aaaagctgga
     1381 agggattctt gctgttgaga ttgatatgcg tcagtagacg caaccctcgt tcactgaggt
     1441 gttttgagtt gagcgagtcc ggtcgggtaa gcacgaaaac agaatgccca gcctgtttaa
     1501 gcaattgcgc aattgcagcc gcatacgcag cttccgcgtt ccaccagtgg acgttgctgc
     1561 cgataaggat cttcattaat aaattgcgtt tcggggaacg cctgtataaa ggataacgta
     1621 tcggtaattt ctcccagacg aagagaaatc aaatccatgg ttgcgacagt cttttcacga
     1681 tcgttttgct tcattaacaa aactttttac tgcccgcgca gttgcttggc aacttcaatt
     1741 tctgagttct ccactaattc agaaagctca tcaacagaaa tcatcatgct tttccggtca
     1801 ggcataatgg cataaagata tttaacatat tcaggaacca ttgttaaata tttttgatta
     1861 tgctccaact ttatttgacg caccataaaa tgctccatct gttcctgcga catatttcgc
     1921 atcacgttta tgaacgcagt ggaactttct tccagttcct tactaatatc agtaggataa
     1981 aataatttta actgtttcct ggaaagtaat attcttacta atcccagcat aaccaggcaa
     2041 accacaaatc cgtcaaccca atgtactccc aaatatccca tgaaacccag caaaaaatac
     2101 actgcacctg tgatcgagcc cagcaattgc agggtcattc tgactttttc aacagaaagg
     2161 tacttgaaat tgtaacgttt ttcctgaaca aatagtaaca atccatcagt aaattgtgga
     2221 gcaaccaaga cgatttcctc cgcggaagca agctggtttt tcaaatccag ttcaagctgt
     2281 tttctttttt caagtgctcg ttcattaatc acttcctgca ttacatatcg acgggttgaa
     2341 cgcagtcttt cacataaatt ttggatatgt tcattcgggt tttcttctgt tccagacata
     2401 ggacttggtt taaatatccc ccgatcacta atataagata ttacatcatc atacagatcc
     2461 tgcatatctg tgggaataat actgctgccg gtttcttttt cctcagccgt agattttgct
     2521 gacaattcca tttcattacg aatgaaaata gaaaatatat tagaagcaat ttctccatta
     2581 gtaggattaa gttctagcat gcgaatgaat atgttgctga caattgaagt ataagttgag
     2641 tattttttcc ccttgtattc aatatccggc ttttcaagat tataatagcc ctttataatt
     2701 gaattaatca tcatgtgcag gaatgtatca taatcttcat tgttggcact ttggttggca
     2761 attgccaacc agctcaaggc tgcatattta tctcctgctg actgagtttc cagccacatt
     2821 tccgtggcat caataaaata gccaacttta cccgtggttc cagtattttt ccaaatataa
     2881 gcagcagctt cggcagatgc agtatcgaga tccatttgct tctgaaagat caccatcaga
     2941 tccctggcaa tctgtgcagc aaaattttcc ggtacatctt catcaactgt tacttcagtt
     3001 tcttcttcag ccatgatcta ttgaattcag ttttcaaaca ttttttattg atcatcggta
     3061 cgccagtctt ggcgcttaag aatttcttcc tgcaaaagct ctaaaaaagc atcaaacgcc
     3121 aaggtcttgc gatccttact tccgtaacgc tgccaggtta ctgtttgtcc tgcttgctcg
     3181 ttttctccca caatcaaaat attcggaatt ttagcaacag cagcagatct gattttttta
     3241 ctgaatgaat cgtgagaatc atccaattct gtacgcacaa acgaactata caaactgtca
     3301 ttcaaaccat gtgcataatc attgaaggcc gatgccacag gaatcacccg tacctgcaga
     3361 ggagccagcc aggttgggaa tgctcctcca taatgttcga tcaaaaaagc gacaaatcgt
     3421 tcatgcgttc ccagaggtgc acgatgaata attatcggtg tgtgctcctg attgtcactt
     3481 gcgatgtaag tcaaaccaaa gcgctttccc atcaccaaat caagttgatt tgttgaagca
     3541 gtttcttccc tgccaattac attggttact tgaaaatcaa ttttcggtcc ataaaatgcg
     3601 gcttcccctc gcactgcttc atagggaact ccaacttcct ccatgacttc cgtaataagc
     3661 gtttcagctt tttgccagag ttccggttca tcgacaaatt ttccagtgtc tttttctgct
     3721 aaggaaaggc gcatccagaa atctttcaat ccaaagagtt catagtattg agtgtggaga
     3781 ttcaaaacat tgagtaattc tgctttagcc tgttcaggag aacagtaaat atgggcatca
     3841 ttcatggtca tgccgcgaac cctcaataat ccggccagtt caccggattg ttcgtagcga
     3901 tagcaagtgc catattctgc caagcgtaaa ggtaaatcgc gataacttct cggctctgct
     3961 ttgtagatca tgtgatggtg ggggcagttc attggtttta gaaacaaacg ctctccatcc
     4021 atttccatag gcggaaacat actgtcttca taaaacggca aatgtccgga aatatggaat
     4081 aattcttctc tggtgatatg cggtgtggaa acccgtttat atcctgccag gaattctttt
     4141 tccttggcca gtttttctaa ttcttctcta atcacagttc cgtttggcag ccacaatggt
     4201 aacccttttc ctacttcttc actgatggta taataatgtt gtttgctgtt caattttctg
     4261 tgatcccgct ccatagccag ttgacgcttt tctttaaatt ccttgagttc atctcctgtc
     4321 tcaaaggcca gtccataaac cctttgcatc atgttgtttt cctcagaacc tttccaatag
     4381 gcacctgcca atgaatcaag ggaaaaacaa ttctttggga tatcgcgcgt ggaatccaca
     4441 tgaggtccct cgcacaaatc ccaaaacggt ccgttacgat aaagactgag aatctcattt
     4501 tgtgcagaaa gatcttcaac ctgttcaatt ttgtaaccct gattatcttt gtctaaccgt
     4561 tccaccattt catcatgacc cagttcttcg cgctcaaaaa cctgtccttt cttaataatt
     4621 tgacgcatac gcttttccag catcggcaag tcttcaggac tcaacggttc agtcaaatca
     4681 aaatcgtaat aaaatccgga atcaatcggc ggaccaaatc cgagttttgc gtcaggacgg
     4741 atttccaaca ctgcctgagc caaaacatgt gccagagagt gtcttatttt ataaagatcg
     4801 gaattgatgt ctgttttttt catttaatat atcagtaagt tatgctataa attatatact
     4861 gtagatttta tatggtttaa agtcaggcag gatgctggac ctctgctgta tcattccatg
     4921 aaatgtccgg aacaattttt tgttgatctt tcacaaaatt tggatgagct tctaaagcca
     4981 aacccaaaga ttctttgaca tttttaactg ctttaatttt caaacctttc ttgatttcat
     5041 ccggaatttc cacaagatct ttttcgtttt cttccggaat aagaactgtt tcaatgagtc
     5101 cccgtttggc agcaataagt ttttctttca aaccaccaat ttccgtaaca tgaccgcgta
     5161 atgtaatttc accagtcatc gccacgtcat aacgtacaga tattttggta aagctgctga
     5221 taatggccat catcacggga agtcctgcag aaggaccatc cttgggagtt gcaccttccg
     5281 gcagatgaac atgcaaatct gtttttgaaa atatttccgg atcaataccc aaatattcag
     5341 cattactgcg aacatatgaa aaggctgctt tggcagattc ctgcatcaca tctcccagcg
     5401 taccagtcaa aacgactttc cctgaaccgg acattaaacc aacttcgatg aggagcaatt
     5461 cgcctcccat tggggtaact ccaagtccca tcgacactcc aacctcgttt ctttcttccc
     5521 gctgatcgtg atgaaattga gggacgccca acaggtcgtg cactaatttt ggtgtcacta
     5581 tagtagtttt cttttctttg gttcgtacca gacgtgtaac tatttttctg caaattttac
     5641 ctatttccct ctccaaatta cgtacacctg cttccctggt ataaccctga gtaatttcct
     5701 caatagcaga ttttcggaaa cgcaattgtt tttcatccaa accatgaaat ttacgctgac
     5761 gtggagtcag aaactgagtg gcaatatgtt gtttttccaa ttctgtgtat ccagataagt
     5821 ggataacctc catccggtcc agcagtggac cggtaatatt ttgaacactg tttgccgtgc
     5881 agataaatag aacatctgcc aaactgtact caacatccaa atagtgatca acgaaggtgt
     5941 tgttttgttc cggatccagc acctcaagca tggctgcaga gggatcgctg atattgcttt
     6001 gatataactt atcaatttca tcaataagca aaaccggatt agtgcttttg gcttttttga
     6061 tagactgaat gatttttccc ggcatggcgc caatataggt gcggcgatga ccgcgtattt
     6121 cagcttcatc tctaacacct cccagactga tgcgtacaaa attacgtccc agtgcacggg
     6181 ctaccgattt ggccagggaa gtctttccca ctcccggagg tccggtcagg caaagaatag
     6241 gtcctttcaa ttttcctttg agtgaagcca cagccaaata ttcaacaatg cgttctttaa
     6301 ctttttccag tccataatga tcttcatcaa gaatcttctc agcctgttta agatctaagt
     6361 tgtcttcggt ccgtacctgc catggcatag agaccaacca ctccaaataa ttacgtacaa
     6421 cattagcttc agatgacatt ggcgacatca tccgcaattt tttcaattct ttcttcgctt
     6481 cactttccac ttcttccggc atttttgcat caatgatctg tttgtccaaa acatccattt
     6541 cagccttagg atcatccatc tgtccaagtt ctttttggat gttgcgcaac tgatcttgaa
     6601 ggaaatcctc tttcttggta tcacttatct gatctgttgc ctctttcttc aatttatctt
     6661 caagctgtac gattttcatt tcatctttca aaatatccag aacctgctgc agacgagttt
     6721 caggattcaa cgtctctagc agctgctgtt ttttatcacc tggaatttcc agcaatgaag
     6781 atactttgtc actcacctgc aggaaatcga cctgatccaa agagtctaaa atttctttac
     6841 tcaacttaat tttgcgctga tccacgtggt ttttaaacaa ttcacgcacc atttctgctt
     6901 gtttcttcaa cttttccagg tcaatatcaa aagcattttc atctacaggc tgaacctgag
     6961 ccagaaactc tttttcatct aatttagtag ccagcaattt cccccttcgg ctaccctcaa
     7021 agagcgcctt tacagtgccg tttggaagac gcattacctg aagtacccga cccaaaactc
     7081 caaatttata caaatcgttt tcgtccggat tttcaactga agaatcccgc tgagtaacga
     7141 caaaaattgc tctatctccc gcaagggctc gttccaatgc atccatagaa gcttgacgcc
     7201 cgacaaaaaa cggaacggtg gctccgggaa agacaatctc ttccctcatc ggaagcaatg
     7261 gaacgatcac aggtttaagt tgtacagaca ttttatttgt ttttagttaa aaggttaatg
     7321 tattagcagt ttaagcctgt agtttttaag caattcttca gcctaatatc ctgatggctt
     7381 aatttatttt taaaggactt cattttgtct taattttctt ctcaggattt tgaaatgaaa
     7441 gtgtcaaata gaaaaaccaa atttcggcca cagcaagcag caaaaggaag attaaataat
     7501 tattgacata gagcgctccc gcaacagttg caccaacaaa atgaccgata aattgtgcga
     7561 aattatatac tcccatcgca gtacctttag tttctgaagt tgtactgcga gtgacgagag
     7621 aagggaaaat tggttcaaaa acattaaagc ccataaaaaa gaaataaaga gcagctacaa
     7681 accatatcag tcggttctgc tcctgtccaa gacccatgaa aaccagcgat gttagtagga
     7741 gcacaattcc aaaaagcatc acttcacgga aacggttctt tacctctgca aaaattgctg
     7801 ccagtaccat ggccaccaca cctgccagca tcatcggcaa gtatattttc cacatctcag
     7861 cacgttcaaa accgtgctgg accaaaatca ttggtatcag aaaaaatgta gatgacagac
     7921 cagcaccaca aacaaaacca ccccagctaa tcgtccgcaa agaaggtact ttccaaacaa
     7981 ttttgaacat gctccaaaaa gaagtgtgtt cgggtagctg ctgtgttgtc tctggatttg
     8041 ggactacagt caacactaaa actaaactga ccgccgacag gagtgcaatc atccaaaata
     8101 ttccatccaa acctatccac tctcccaaaa aaggagctgc cccaaaagca acggcaaaag
     8161 caatgccgat gcttattccc attgctgcat tggcacgtgt acgtacttcc ggacgtgtta
     8221 aatctgcaat caaagcaaat acagttgaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn cgtgtacgta cttccggacg tgttaaatct gcaatcaaag caaatacagt
     8401 tgaactgatt gcaccggtgc cttgaagaaa acgggccaca atcatccaat gtatcgaatc
     8461 cacaaaagcc gcaaggaaat tgccggcaat aaacaatccc aaaccaatca caatcaccag
     8521 tttccgtcct gctctgtcac tccacattcc aaatggcaat tgaagaaacc cctgtgtcaa
     8581 tccatatgca ccaaatgcta ctccaatcag aaaaggagtt gcaccttcca aatccattgc
     8641 aagtacacta aaaaccggca acaacagaaa aagactgaac atcctgaaaa aaaggacccc
     8701 gctcaagctg taaatgctcc ttttttctga caaagtgaaa attggggttt ttgcagattc
     8761 gctcatttat tatttttgtt ccagttcgtg cctccttttt ttgaggaaaa gcaaattata
     8821 agtaattaga ttatgacaac ctaaagcttg aaattcaagc tttagagaaa aaatgcagac
     8881 atagatttta taaaatgttc agtcattgac ttgcaataca tgtaatcata cacttggaaa
     8941 aagtggtaat tcgagataaa caaaactgga aaaaataagc agattatttg tgaatttagg
     9001 aattaaaact tgattcaagt ctcaacttat tctaaatttc aatgagctta tttaattcat
     9061 gaaacaaact gcctcggttg aaaaagtaat tagacaaaaa cagatcgtga accagttggt
     9121 caatttgtga cattaagtgt gctgctcctg ctagaaaata acaaaactca aatcagtccc
     9181 aatttcacag agaactttca ggatcttcta gaacagaaca aatgattgac gaaaattcct
     9241 ccaccgtcat tgttaacatc catggcttgt tgggagaaca agattgcatt caaatggatt
     9301 ttgaagaaga gcttcttgta gaggaagagc aatttattat tgataatgtg gcttacgaaa
     9361 ttgtccgagt cataaaagaa gatgtcgagt atcctgttgt ttacgtcgta attctcgaca
     9421 ttctcaatca tacctgatct ttgttccctg tctttgttct ttgccatgac atgcctgcaa
     9481 cactccaaaa aattctacag aaaataaaaa cagactctac ggttgttgaa ttacagggac
     9541 tttcaggatc aagcaaagcg ctggtagttt ccatgctcag ccaaatacct gaacagcctg
     9601 cagaaaaaat aattccgctg gttgtggtct gtgagtcttt tgatgttgca gaagtcctgc
     9661 tcaacgacct ctactatttt tttggaaaag aaggtgtaca ctttttcccg ttctgggatg
     9721 tgctgccctt cgataatttt tctccacaca aaggtctggt cgcccagcgt tttcaaacac
     9781 ttgatgcatt actaaactca gaggtacgag tccttatcac aactccaaac gggatgatgc
     9841 agcgctttct gccccgtgcc gctttccaaa aaaacactct ttctctgagc accgattttg
     9901 ttggtggaaa gcaggaactc cggcaacaat tgttaaattc cggatacatt caagtggatg
     9961 ttgttgaaga tcagggagaa ttcagtgctc acggagatat catggacgtt tttccgctga
    10021 atcaggaaaa accggtgagg atggagtttt ctgaaaattc agaacttttg taccttaaac
    10081 catttgagat tcaaactcag cgtacagctg aagcggaatt gacttcgcta aaaatacttc
    10141 ctggcagtga aatacttttc aaccaggaga ctatttattt tgcccgtcaa acactgccgt
    10201 catatcggaa agaatgcact ccggaagtct tgcgccgatt gaaggaaagt ctgcagaaat
    10261 gtgagagctt ccccggaatt gaatctctct ctccgctttt ctatccaaaa ctggaaacac
    10321 ttttcgacta ttttcctgca gaatatttgc tggtggttga tgaagaaaat catataacgg
    10381 agcgggcaga gcatttttat caggaagtat ttatggaata cgaactcagt aaacagcaaa
    10441 ataagctcac tctgtctccg gaagcattat ttttgacaca cagggaactg gagagtcgtc
    10501 tcaaagaatc cgcacaagtt tatttaaaat caaaagtccc gggtaaaaaa tcagagcgta
    10561 ccatttatca acttcaattt tctgacaatc agtcccttcg tcctggattt gaacattcaa
    10621 aagcaacttc tgctgctgga catatggtac agctgcttca ggactggagt aagtcaggga
    10681 tcccaattat tctaagcgca aaaaaccaaa cacatgcaga tcattttcag cagttgctgg
    10741 aagatttagg tgtggaaagt actgttgcgg gaaagcaaca agttgcgaaa gactgtccct
    10801 ggccgaagtg gctggaaagc aacactgttg acggattgaa agagaagatc ccgattttat
    10861 gcggaaatgt ttcttccggt tttcggagac ttgatgcaga tggccaaaca caatttattt
    10921 tactgactca ggaagaggtt tttggtgaaa aaaaacgaag tagacggttg cagcgtacgc
    10981 aagttcagca ggtcgcagga aatttggatg atttgcggga gggtgatcat gtcgttcatt
    11041 tagattacgg aattgggcgt taccacggtt tgcagaaaat tactgcagga ggttcaaaca
    11101 acgaatttat gctgctcatc tatgcgcgtg aagaaaaagt ttatgtgcct gttgctaaat
    11161 ttcacttggt acagaagtat gtcaatgcag acggaactgc tccaaaactt agcaaacttg
    11221 gagaaaaagc atggaaaaaa acccgtacaa aagtagcacg tgcagtagat gacattgcag
    11281 aagaacttgc ccagatttat gctgaacgca aagcacggaa gggttttgcc tttgctccgg
    11341 atgatcatga aatgcgtgaa tttgaactgc gctttccttt tgaggagact cctgatcagg
    11401 aggaagtaat aaggtctgtc aaggaggaca tggaaacaaa gttgccaatg gaccgacttg
    11461 tctgtggcga tgtaggtttt ggaaaaactg aaattgccat gcgcggtgca ttcaaagctg
    11521 cacaacagga aaaacaagtc gctatcctgg taccaactac aattctggct cagcaacatt
    11581 acgaaacgtt cgtcaaacgc tttgaagata caccattcat cattgaggtc atcagccgct
    11641 tcaggaccga tgcagaacaa aaaattattc ttaaacgtct ccttgaagga aaaatcgata
    11701 ttattattgg tacgcaccgc ctgctttcga cagatgtaaa gttcaatgat ttgggtttgc
    11761 tggttgtgga tgaggagcag cgctttgggg tcaaacacaa ggaaaaaata aagcgtttca
    11821 gagcggcagt tgatgtactc acgctttcgg caactccaat tccacgtacg cttcacatgt
    11881 ccctgatggg tatacgtgat ttgagtttgg ttaacacacc tcccgcagac cgcagggcga
    11941 tacgaacccg gttgctgccg gtcaatgatt atattattca ggaatcagtc agccgggaaa
    12001 ttcgccgggg gggacaagtt tttgttgtac ataaccgcgt tgaaacgatt tatgaatatg
    12061 gacgttacct ggaaagtatt cttcccaaca tccggattgc aatcgcacat ggacaaatga
    12121 gagaacaaca tctggaaaaa gtaatgcttg attatattga aggccgcttt gacgtactgc
    12181 tctcaactac aatcattgag tccggacttg atatcccgag agccaacacg atcatcataa
    12241 ataacgcaca aaattttgga ttgtcccagc tctatcaatt gcgtggaaga gtaggtagaa
    12301 gcgatgtgca ggcctatgcg tatctgcttg ttcctgcaga aaaaatacta agcggagtag
    12361 cacatgaacg tatacaggtg ctgcaggatc tcaatgatct tggcgcagga tttaaagttg
    12421 cgtcaaggga tttggaaatc cggggagcag gaaacttact gggctcagag caatccggac
    12481 aaatcgcaag tgttgggctc gaactttaca ctcaaatggt ggaccgggct gttaaaaaac
    12541 ttctgcaaac cgaatcaggt ttggctccgg aagatattca ggtcagattg gatcatattg
    12601 aacaatccat tccagaatcc tatattcaca gttccagtca ccgcctttcg ctctacaaag
    12661 cgttgggtac gttaccgaca aaggaagagc tgtgggaatt ccgtaacggt gttgaggatc
    12721 gttttgggat actcccggaa tcagtgctga atatttttag gaatgcagaa gtcaggcttt
    12781 gggggcaact ccatggagtt gagaaaatag aacatgaaca aaaccgttta agaatacaag
    12841 tcaaagatgc ttccagactc aatcatgaga aacttgttgg ttggctctgt gagcaggaaa
    12901 caccgttgag ttatattcct gaaaatactc ttgactataa aaatgtgcct ccggaaatga
    12961 atgcaatcct taaaggtcta aaaaatctcg agcaggtttt taatgtttac ggttgaaagt
    13021 ttaaggtcaa aagcggttgg ttaattgtca aaatgccaga ataaaaaaag caaattttta
    13081 actgctcgac agtcccgagt tttgtagtgt aatcccgaaa gtatgtgaag gaccttatac
    13141 gatatccgta ctttgacaga tctctctttt aggtctaaat gacaagccgt agccaataat
    13201 ataagtattt tacgagaaca cgtgactcac gagtaaactt aaaaaaacga aaaacaggaa
    13261 cttttgattc tttttgaaat caacttctgc agtctgaaga acagtaaagt ctgtccggat
    13321 tatttctgga acagcttgag ggattgctga ggaaaaagac tgattcccgg agtggaatat
    13381 cagcatcaaa aacaggtaca cgggaggcca ttgaataaag cttgtcgtcc ctggcaattt
    13441 tttcccatcc ttgggaaaca gtggcctccc atgagatcac ttaattagat cagcaagtat
    13501 gcaagtttcc caccaattcc agctatgttt gtttaagagt ttaaaaacct gctgtaaact
    13561 tccataaaac gccgcatatc cggcatcatc ctgagactca tccgaaaagt gtgggaaatg
    13621 ggggcgctca tcatccggac aagtatttta ttttccttca aaaatgtagt tgctttgaca
    13681 atattatggg gttcaaccaa cataaaattg gctgctcctg aataatattt gacctgctgt
    13741 tcgtcaaaat atttttcaag tgcaggttta ctctcatcca ttagatgtct tacaactcct
    13801 tgccaatgct caggatgatt cagttgttcc tgcgctgcta aaactgcaac caggttcaca
    13861 tcataaggtc cacgtatttt tttgagatga acaataaagt ccggcctcgc aattacatag
    13921 cccaaacgca aacttggcaa acccaatgct ttggaaaaag tgcggattat tatcaaattt
    13981 ggataatttg gtaatagagg tacacaagtt tgtccggtaa attcaaaata ggcttcgtca
    14041 accagaacag gcacatcagg aaatgttttc agcaaatcct caatttgatc ctgcgaaaca
    14101 gaggttccag tgggattatt tggatttacg accacaatta aacgggtctg tggattcact
    14161 gcatctttca attcttcatg aggaaaatga agatctggag gaaataaagg accaactacc
    14221 tgacacccta ttgttcctgc tacttgctta aacattgcaa atccaggttg aaccattgcc
    14281 acatcctcac cactttccag taaacagcgc aaaatgacgt ctattgcctg atccgaacca
    14341 tttgtcagca gtaaactgtc ttgggaggca ccggtatatt tactcagcac ctcaagaaaa
    14401 tcgccgtaga caggatatgc atggacgcgc tttttcagga aatgagctat tttatcgata
    14461 acttctttgg gaggaggaag cggaggttca ttgaagtcca tcaacaaatg ctcttccgga
    14521 ttacgattct caagaggagg atcgtagaaa ctcatccggg aaattgtatc ccggactttt
    14581 atactttgct gcagatttga gtctgacacg tgttctcact ctccgattga aaacatgtgt
    14641 tccagatcgt gtcgcgaaaa cgtgttgaaa gcaattaatg tttctgtagc agtaattcca
    14701 ttaagttctc caaattttac tgttattgta tccgcaattt gctgattgtt tattaccagt
    14761 acaatcgcca ctaaatcata cctgcctgca accgaattga cctctgagac ttcctgcatg
    14821 taaactagtt gttgcgaaat taatttaatt tgttgacgct ctatatttaa taatacaagt
    14881 gctgtaacca tgattttact cctgtaaagt ggattgtttt ctgaaaggat tttttattgt
    14941 aacaaagctt aattattttc aaaagtttat taaatgacgg tcttgtaata agtctgaaat
    15001 cctcacagtc atttcgactg tagggagaaa tcttaatgat attagtgtga ttgcttcaac
    15061 aagatttttc gcttcgctcg aaatgacacg gttttaggac tttttactac tgcatcatta
    15121 agtaagcact gaacaatttt tcggaaggag tgacctgcaa acatacacac gcgcaaaggt
    15181 cttatttcaa gaacctttgt ggatctgaat caaaaactaa acagttccaa acaaaaatcc
    15241 ggtttattca aatgacattt aaactggcag gagccagaag cattcagcta atttcaacat
    15301 tttcaaacga aagaataatg caccaacttt tctgaccaac tattaatatc agcctgcttt
    15361 gacaataaaa atcattttct tgttagaaat gtttccgagg ttgtcttcgg cttcgacgat
    15421 aacgtaataa ttaccaggct tatccagcaa caaagcttcc tgataagcct ctttagggct
    15481 tccgttaata ctaaaacgaa ttccctttag cttagcagaa ttgtcatctg cattgaggaa
    15541 aagtgtagta tatagtgggt acacgtctat tttatcttct ccatctcctc cttcactacc
    15601 aactgcagac ctgttgaaga cagccttaat ttctggtggt gtatcatcaa ctacaaggag
    15661 cacaggtgtc aaaacctctt tattgttgac atgatcaact gatgagtatt gaaaagtgtg
    15721 gcgtccttca tctgaaatag taaatggacc tttataaagc aactctttat tttcattaaa
    15781 tttatagaaa attgactgaa ctccagagac attatctaat gctttcagtg agatacgagt
    15841 ttttgtgttc acccagatta cattgggacc acgcctgtaa gtcactccat cagtaataag
    15901 ccgtgtggtt ggactttgtg aatcaacgac aaaatttgct gaagcaagaa tagatttgtt
    15961 accaattata tcgattccct gatagtccat tttgaattca cctgtttgta ccggtggtcg
    16021 aaaagatatc ccgtagttac catatgaaag accatttacc tggtattcaa ttgactgaat
    16081 atcaaccatc tcgtctactg ccttcagaaa tatttgggaa cgtgaagaca aataaacttt
    16141 tccaccttca atatactgat caccttttat ttcatgcgaa acttccggac catttttatc
    16201 aaaaccgaga gttactttgt gaatcccttc agtgttacct actaagtctt cagtataaaa
    16261 ctgtaatata tttgcacctt ctttcacacc ttcaaataat attctatttt tataagacct
    16321 tttaaacact cctgaattcc aattatagta agtaagatag acaccggaaa gtgcgtcttg
    16381 acttggtatt gacaaagttg attttggacc aaaaatattt ttggtggcat taaccatttt
    16441 caaatttgtg acaggtggag ttttatcaat gtaaaaacga gtctgtaaag tttccgaagt
    16501 atttcccacc ctgtctaccg catagtgtcg cagcagataa tatccttctt cggagaattt
    16561 cattccatca gaaggtaaaa atgttttccc attgactgaa aggtaggttt gctccacgcc
    16621 tgaaaatgca tctgtagcac tcaaagttgc gattgcatca ccggaaaaga acagccccct
    16681 gatatccatt agtcgttttt ttgaaacaaa ttgcaactga ctttctggag caactccgtc
    16741 agcgatgaag cgcatcgttg tttctttttt gttgtagtga tgcagccagc gaacaaactg
    16801 attactgctg atttccagtt taagcccgtc acttcggtat ttttccaatt catttgctgc
    16861 atctggtttc caatgtccag ctgagttcgt ggtaccagaa tgtaatagtg ggaagagttc
    16921 ggctttctcg tcactggaag ttccaagcca aaaaaagata ggcttatcct ggggccagta
    16981 aataaatttt tcgtcgttta cataaacatg cagtttatgc tcatttggag gcgctaagag
    17041 cattttaacc acagcaggcg tcgttgcatc tggatcaacg cttgactg
//
LOCUS       DTVD01000245           17038 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_622_length_17038_cov_403.201025, whole genome
            shotgun sequence.
ACCESSION   DTVD01000245 DTVD01000000
VERSION     DTVD01000245.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17038)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17038
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_17038_cov_403.201025"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="EYO68_09075"
     CDS             <1..390
                     /locus_tag="EYO68_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HIC07988.1"
                     /translation="LSIGVVLGFMGWMGIPLDMMTITIAAISVGIAVDNTIHYIHRFR
                     HEFAIDRNYLAAMHRSHESIGYAMYYTSITIIIGFSILVLSKFIPSIYFGLLTGLAML
                     IALMAALTLLPQLLIFLKPLGTEKQEV"
     gene            508..1449
                     /locus_tag="EYO68_09080"
     CDS             508..1449
                     /locus_tag="EYO68_09080"
                     /inference="COORDINATES: protein motif:HMM:PF03547.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIC07989.1"
                     /translation="MYGPVIDIILPIFGILLIGYTTASFGWFDQTAVRGLTRYVFDFA
                     VPMLLLRTLATTSLPEVIPWDYLAAYYLGTLTVLFTGFFITWVFWKRTFSERVINAFS
                     GCFSNTVLLGIPVILLAFGERATLPLFMIIGTHGLIMLPLFTIMLEMGKSGRAPIPTV
                     IARTSYGLFTNPLIIGLLSGVACNLFEITLWKPLDEMAKLLGDSVTPGALFALGATLS
                     GFRGKIQWQEVPLIVIMKTIIHPLIVGVLATQVFDIRNTTWVQVLVMLAAMPTGVNPF
                     LFASRYNVGQSVSSGTVFISTVFSIFSLSVLLAYFQQ"
     gene            1513..2196
                     /locus_tag="EYO68_09085"
     CDS             1513..2196
                     /locus_tag="EYO68_09085"
                     /inference="COORDINATES: protein motif:HMM:PF00378.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIC07990.1"
                     /translation="MAVLEFEKKGNIFIVTLNNPATGNAINPEALSAHREVLGELEAV
                     SENSAVIVTSSDPKSWCVGLDFEWLKGQSAEEFAGTFRELEEVFGRWALLALPTVGCI
                     TGHCLAGGAIFASVLDFRLMRSDRGWFAFTEIDVKIPLSPILYEMADLLPNKQAVREL
                     LLTGRRVGGADAQKLGVVDEAHLPEMLMPRALEIAEELSQKDLKTYNAMKQLLKQNLS
                     KQIAEGSNA"
     gene            complement(2324..5443)
                     /locus_tag="EYO68_09090"
     CDS             complement(2324..5443)
                     /locus_tag="EYO68_09090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIC07991.1"
                     /translation="MKEVSNKLSFAQMEEEILNFWRQKDIFQKSIDERPKDKPFSFYD
                     GPPFATGLPHYGHLLAGTIKDVIPRYKTMRGFRVERRFGWDTHGLPVEFEVEQSLGLN
                     GRHEIENFGEGNFNEECRSIVLRYTGEWRETVERMARWVDFDNDYKTMDIDFMESVWW
                     VFKRLWEKGLIYEGVKVVPYSWRVSTPLSNFEANLNYKEIQDPSVTLRFKINGEENLY
                     FLAWTTTPWTLPANMSLCAGSKLTYCKIRDKKSGDCYLLTKNRIESNFDDGSYEVLTE
                     MKGSELKGMTYEPLFDFAKAKVDTTNAWSVQLDDYVTDESGTGIVHLACFGEDDVRIF
                     QRENISIFDPVDDEGNFMDYMEFIAGMNIKDADKSIVKRLKEEHKMFRHETAQHSYPF
                     CWRTDTPLIYKPISTWFVNVEAIKDRMVAHNKTVHWVPGHIRDGRFGKWLDNARDWAI
                     SRNRFWGTPLPVWKSEDGDVWCFGSVSELEEASGEKITDLHKHFVDPLVIEKNGKTYR
                     RVPEVLDCWFESGSMPYAQEHYPFENKERFEANFPADFICEGLDQTRGWFYTLAVLGS
                     ALFDTPAFNNCVVNGLILAEDGKKMSKRLKNYPDPNEMLNKYGADAVRLYMLNSPAVR
                     GEDLRFTEKGLVETTRQQLLPLWNALVFLSTYAKIDGWDPSPENLSVKNSNPLDRWIL
                     SRLHKLIAEVQKEMDGYDLNRSVAPFVGFIDLLTNWYIRRSRRRFWKAGQGHDKREAY
                     ATLYTVLFEFSKLIAPFIPYIADGIFRTLRHDTDHQSVHLAFFPEANSAKRDLDLEKR
                     MDLVLSTVTMGRALRAKHQLKIRQPLPKIFLVTRDPEAKNVLEELSELISDELNIKEV
                     VITQDEEQLVNLSAKANFKTLGRKLGKKMKIAAQLISGLDFENIRTLQNGKNIILKMG
                     EETLELTPEDILIQRQEKEGLLVETDNLLTVALDTELSENLIQEGFAREFINKVQNMR
                     KDMDLDVMDRILISYQSSAKLNTALDAFSDFICKETLANRLQPQGTLDGTEWDLNGES
                     CKIKVELNL"
     gene            complement(5522..6526)
                     /locus_tag="EYO68_09095"
     CDS             complement(5522..6526)
                     /locus_tag="EYO68_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07992.1"
                     /translation="MTVTQPEWVEALKRPAAYLLKSPEKIKLLETTTSYLFRADDWLY
                     KIKKMGNEYPTLAVKEAFCREECSLSQRFNPNWAVEVLPVTEHNGVIKFGGTGGTTIE
                     YALKMEALPDQWQLAQLLDKNKITTNNMVGIATKIADIHAMSPAKDKEAETGKPEPFR
                     AQCDDLLFQLKRYFEASLTQPILDMIRHPLEKFIDDNKRLFTKRMRNGRIVLGHGAFL
                     PEHIFLNGDVIRFISPQEIQKKLAVLDVANDVSSLTVELSRLGKTELLDSFVKQYLEI
                     SKDKDLLKMLPVYQTYCALKQGVKTCELKVAQKDESLGTLAMDYFNLAVRFSREIPRN
                     "
     gene            complement(6719..7933)
                     /locus_tag="EYO68_09100"
     CDS             complement(6719..7933)
                     /locus_tag="EYO68_09100"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIC07993.1"
                     /translation="MITKDDFFNSIEDAIKAIARGEIIVVVDDEDRENEGDLICAAEA
                     ATPEIINFMAKYGRGLICLPLDAETVERLNLPLMVSNNNETMGTNFTVSIEASVGVTT
                     GISAADRARTIKTAIDPESTVHDIRSPGHVFPLLARQGGVLRRAGHTEAAVDLAQMAK
                     MLPGGVICEIMNEDGTMSRVPELRKFADHHGLLIITIKDLIEYRKRNETLVIKKAEAL
                     LPSKFGEFKVIGFQSVLDDSDYVALVKGEWEEDEPVLVRVHSECLTGDVFGSYRCDCG
                     PQLHAAMEMVESEGKGVILYLPQEGRGIGLINKLQAYKLQEEGADTVEANKKLGFKDD
                     LRDYGMGAQMLFMLGVRRMRLMTNNPRKIVGVQGHGLEVVERVPLEIQTNPGNKKYMH
                     TKKHKLGHILNI"
     gene            complement(8106..8762)
                     /gene="ribD"
                     /locus_tag="EYO68_09105"
     CDS             complement(8106..8762)
                     /gene="ribD"
                     /locus_tag="EYO68_09105"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIC07994.1"
                     /translation="MTKNSGIIVTLKAAATLDGKIATATGHSKWITGEAARQKGHELR
                     NENDAVLVGINTVLTDDPELTVRGIDGGRSPVRIVLDSKGRIPEQSRVFQQDGVPVVI
                     VTGNHAPLRKWPELAELTILTAPTRTPDILWVLSEIRKLGMNSLLVEGGSLIHASFIK
                     SNTVDQLALFLAPKVIGGQEALSWCGNLGVDSVDDAPQMEISSVTALGEDWMISAIIK
                     "
     gene            complement(9019..10029)
                     /locus_tag="EYO68_09110"
     CDS             complement(9019..10029)
                     /locus_tag="EYO68_09110"
                     /inference="COORDINATES: protein motif:HMM:PF03959.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC07995.1"
                     /translation="MKVLYRVFLIIVCACIIVTSVAAFKIYRLQQPFQAADVPEWKVK
                     ELSSQKWELKSITDPERSTTAWIARPEGQKCDTLIIVGGVEEGENLLAGGGNWKYTGN
                     TILMKQPVHSFLLRHHWKDWSLLDWWQIPEKIREETRHTLGALNALLNYVHGGTRSDP
                     RFTDKVVLAGGSVGAAFPAILTSFVPEKVAGLMVVYGFTNFQHVIQPLLFSQGLLHFD
                     LTENSADFSTKIKISGVRLLSHILSFVLGNILKYGEMESYLPDIYQTPIQFINGTNDP
                     LVPPEAYLPMWKSAPEPKSEKWVEGGHFNPRAPKDMLNIGKLMYEWAEKQGIRSCYPH
                     IK"
     gene            10098..11138
                     /gene="tsaD"
                     /locus_tag="EYO68_09115"
     CDS             10098..11138
                     /gene="tsaD"
                     /locus_tag="EYO68_09115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIC07996.1"
                     /translation="MLIHMSAIGLGLDTTFDDTSVAILRGKREILSNLTLSQYKEHEE
                     FGGVVPERASRKHLEVIHHIIAGALKEADLDFSGIDYIAVSNYPGLLGSLLVGLTVAK
                     SLAYTLQCPLIGVNHVEAHPYANVLKHGEMQFPILHLVVAGGHTLLIHARGHFDYKII
                     GRSVDDAAGECVDKVAKMFGHSMPGGPVVDRAAMEFPGDVYDFPRPLLHKKAHNFSFS
                     GLKTAMLRFKEKSQSTSSSSFDSPDRLQEDGPVLASFFQSVIDVLIHKTFNAAEAYGL
                     NNISVSGGLAASRKLRLAFEAESARKGVKLFYPPPNLCTDNAAMVTCLAAHRYEAELF
                     DDLTLDAFANLN"
     gene            complement(11152..12081)
                     /locus_tag="EYO68_09120"
     CDS             complement(11152..12081)
                     /locus_tag="EYO68_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HIC07997.1"
                     /translation="MIGIKAIGTYLPEKRISNFDRIEKFDMTESFIREKIGFTEVALK
                     APEQKTSDLCVKSWENLLQQHPVSPNEIDCLIVCTQNPDGHGLPHTSAIIHDKLNFPL
                     NCAVFDLSLGCSGYVYGLSVIKSFMEANNFRRGLLFTADPYSKVMNQEDKKTATLFGD
                     GATVTLLTDEPVFEFGQFVFGSDGSKKEGIIVREDGYVHMNGRAVFTFTVRIIPDSIR
                     EMLEKNSFVLENVNRFLLHQGSLHIVNSIADNLGVPRERCPFHSAAYGNTVSSSVPML
                     IAQEYANQEMQVAVLAGFGVGLSWASTLIFRKN"
     gene            complement(12087..13061)
                     /locus_tag="EYO68_09125"
     CDS             complement(12087..13061)
                     /locus_tag="EYO68_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07998.1"
                     /translation="MWTDVLLILDATLRISVPFVLAAMGGIFAERSGVIDFGLEGKML
                     GGAFVAATVAHLTQSAWWGLLGSIGIGIIFSMIHGYASVSKRGNQVVSCVAINIFAMG
                     LTVVLANAWFHMGGKTPQLPKEARFMSIELPFAEEFRNIPIIGEIYYELISGHNALVY
                     IAFIAVPITAWVVFKSSFGLRLRATGENPLAVDTTGISVNKTRYQALILNGILGGMSG
                     AYLSTAHLAFFVKDMSAGKGYIALAAMIVGKWRPYPAMFACLLFSFLFAIKDKPEIFD
                     LLKGIIPVKLLNSFLDMSPYILTIIFLAGFVGKAVAPKAIGQPFSKEK"
     gene            complement(13149..14240)
                     /locus_tag="EYO68_09130"
     CDS             complement(13149..14240)
                     /locus_tag="EYO68_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC07999.1"
                     /translation="MNERRTETLVVTLVTVTNLLIAFFISGVVIWALGEDPWFALKTL
                     LYGAFGYDEGLGYTLYYTTNFIFTGLAFAIGFHCGLFNIGAEGQAYIGGLGVGLICLW
                     LEDWSLWLVFPLAVLTSIILGGIWGAIPGYFKAKRGSHEVVTTIMFNFIASVIMVNIL
                     SHVLRPPKEMSPESREFAEGVWIPQMHEIFDWFGINITQTPLNGTFFFALICLVLVWR
                     FIWYTRWGYEIRTVGTNDTAAVYAGISVSRNIILAMVVCGGLAGFVGINEIMGSSHRI
                     MLNFQAGIGFTGIAVSLIGRNHPLGILLAALLFGILYQGGAELSFEVQAIDRHLVVLI
                     QGLVILFAGALENLFRPQIEYFVKQLARQ"
     gene            complement(14237..15766)
                     /locus_tag="EYO68_09135"
     CDS             complement(14237..15766)
                     /locus_tag="EYO68_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08000.1"
                     /translation="MSTVAIELTDINKYFGNVCANKDVSLKVESGTIHGIIGENGAGK
                     STLMSILYGFYQADSGAIAVQEKSTEIRSAHDAIAAGIGMVHQHFMLVDTFTVLENVV
                     LGTETGFSLQESMQKTERELQRLEKDYHLEVDINAVINTLPVGLQQRVEILKALYRGA
                     DILILDEPTGVLTPQEADHLFRILNELREQGKTVILITHKLREIMAITDNVSVMRQGE
                     MVAHRKTSETDREELAELMVGRKVLMQVDKAPAKPGSPMLTVKHLDYIDDFGVRRVKD
                     VSFEVHCGEIVGIAGVSGNGQSELMEVITGIKPFQKGQIQISGQTITSTVRADAATMR
                     RFGMAHVPEDRQRRGLVTSFSANENIILGYHKNPSYNNFIEMDRDFIIQSCKKQMEHF
                     DVRPDNPHLRASLFSGGNQQKLVVAREFERDPALLVVGQPTRGVDIGAIEFIHRQIIA
                     MRDAGKAVLLVSVELDEILSLSDRILVMFDGQIMGEILAKDADEREIGLLMAGVRKEA
                     A"
     gene            complement(15846..16787)
                     /locus_tag="EYO68_09140"
     CDS             complement(15846..16787)
                     /locus_tag="EYO68_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIC08001.1"
                     /translation="MPSQKIIIDTDPGQDDAIGILLALASPAELDVLGIVTVAGNVPL
                     SLTSRNALILCELANKTETKVFAGCSRPLVRPLVTAEHVHGKTGLDGAELPPPTMSLQ
                     KQHGVDWTIETLLEAEDNSITICCFAPLTNVAMAMIKAPQILPKIKNIVMMGGGYFEG
                     GNITPTSEFNIFVDPHAASTVLSCGRPLVMLPLDVTHKALMQRKWLEDLKALGTPVGD
                     AAFGMMSFYERFDLEKYGSGGGPLHDPTVIAYLLQQELFEGKFVNVEIEISSELTMGM
                     TVVDWWKVTDRPPNVTYINQINADGFFQLILKRLAIL"
     gene            complement(16891..>17038)
                     /locus_tag="EYO68_09145"
     CDS             complement(16891..>17038)
                     /locus_tag="EYO68_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC08002.1"
                     /translation="GVGWALDEHNAKLVTSAMESKVEQVREGILSGKIKIHDYMSDNK
                     CPVQ"
BASE COUNT         4820 a         3720 c         3390 g         5108 t
ORIGIN      
        1 ctttcaattg gagtggtgtt gggattcatg ggctggatgg gaatcccatt ggatatgatg
       61 accattacaa tcgcggcaat cagcgttggt attgcagttg ataacaccat tcactatatc
      121 caccgcttca ggcatgaatt tgccatagac cgcaactatc ttgccgccat gcatcgttca
      181 catgaaagta tcggttatgc tatgtattac acttcaatca cgattatcat cggcttttcg
      241 attcttgtgc tctcaaagtt tattcccagc atctactttg gtttgctgac aggactggcg
      301 atgctgattg ccctgatggc tgccttgacg cttttgcccc aactgctgat cttcttaaaa
      361 cctttgggta cagaaaagca agaagtttag cataatcatt caaagaactg ttcaataatt
      421 ttactgctcg ccattaccgg acagttgctt tgatgaattc aattcattgg atacttaatc
      481 cggctccatc ttccaaagat tataaaaatg tacggacctg taattgatat aattttacca
      541 attttcggta ttttattgat tggatatact actgccagct ttggatggtt tgatcaaact
      601 gcagtaagag gactaacacg gtatgtcttt gattttgctg taccaatgct tttgctgcgt
      661 actcttgcta ctacgagtct tcctgaggta ataccatggg attatctggc tgcctattat
      721 ttggggacgt tgacagtatt atttacagga tttttcataa cctgggtgtt ctggaaacgc
      781 actttttctg agagggtgat caatgctttt tccggttgtt tttcaaacac agtactgctt
      841 gggattcctg ttattttgct tgcctttggt gaacgggcaa cgttgccttt gttcatgata
      901 atcggcacac atggcctcat tatgcttcca ctttttacca tcatgcttga aatgggtaaa
      961 agcggaagag cacctattcc aactgtcatc gccagaactt cgtatggttt gtttaccaat
     1021 cctcttatca taggactact ttccggtgta gcatgtaatt tgtttgaaat cactttatgg
     1081 aagccattag atgaaatggc aaagttactt ggtgactcag ttactcccgg tgctttgttt
     1141 gcactgggtg ccacattgtc tggtttcagg ggaaaaattc agtggcagga agtacctctg
     1201 attgtaataa tgaagactat tatacatcct ctgattgtag gggtactggc gactcaggtt
     1261 tttgatataa gaaacactac ctgggttcag gtactggtga tgttagcggc catgccgacg
     1321 ggtgtaaatc catttctctt tgcaagtcgt tataatgttg ggcagtcggt atcaagtgga
     1381 acagttttta tttccactgt tttttctatt ttttctctct ctgttcttct tgcatatttc
     1441 caacaataag ctagattatt atcttcaacc aaagaaaatt aaactcccag acattataaa
     1501 ataaagtaaa aaatggcagt attagaattt gaaaaaaagg gtaatatttt cattgttacc
     1561 ctcaacaatc ctgcaactgg aaatgcaata aatccggagg ctctgtctgc acatcgggaa
     1621 gtactggggg aactggaagc agtttctgaa aattctgctg tcatagttac aagcagtgat
     1681 ccaaaatcat ggtgtgtcgg cttggatttt gagtggttaa aagggcagtc tgcggaggag
     1741 tttgcgggga cattcagaga actggaggag gtctttggcc gctgggcctt gttagcatta
     1801 cctactgttg gctgtatcac agggcactgc ttggctggtg gtgctatttt tgcttcagta
     1861 ctggatttcc ggctgatgcg ttcagacaga ggctggtttg catttactga aattgacgtt
     1921 aaaattccat tgtctccaat cctatacgag atggcggatt tattgcccaa taaacaggct
     1981 gtacgtgaac ttttactgac aggtcgtaga gttggaggtg cagatgcgca aaagctggga
     2041 gtggtggatg aagcccattt gccggaaatg ttaatgccga gggcactcga aattgcagaa
     2101 gagttgtctc agaaagatct taaaacttat aatgcaatga aacaactgct aaaacagaat
     2161 ctttcaaaac agattgcaga aggatcgaat gcttgatttg aaaaatggct gaattacact
     2221 ccggacaaat gttcaattaa atctaatatg aattctcagg tctgttgcat taactgttga
     2281 gaagttcgtg caaaagggct gcgtaagcag ggaatgagag gattcataaa ttaagttcta
     2341 ctttaatctt acaggactca ccatttaaat cccactctgt accatcaagt gttccctggg
     2401 gttgcagtcg gttagcaaga gtttctttgc atataaagtc tgaaaaagca tccagtgccg
     2461 tgtttagttt tgctgaactt tgataactga tgaggatcct gtccattaca tccaaatcca
     2521 tatccttgcg catgttctgt actttgttga taaactctcg cgcaaagcct tcctgaatta
     2581 aattttcact caattctgtg tccagggcaa ccgtcaagag gttatcagtt tccaccagta
     2641 atccttcttt ttcctgacgt tgaatcaaaa tatcttccgg agtcaattcc aatgtttctt
     2701 ctcccatttt caaaataata tttttaccat tttgcagtgt acggatgttt tcaaaatcca
     2761 atccggaaat gagttgtgct gcaattttca ttttcttacc aagtttcctc cccaaagtct
     2821 tgaaattagc cttggcgctc aaattaacca gttgctcttc atcctgagtg ataacgactt
     2881 ctttgatgtt aagttcatcc gaaatgagtt ctgaaagctc ctccaaaaca ttttttgctt
     2941 ccggatcacg tgtgacaagg aaaatctttg gcaggggctg gcgaattttt aactgatgtt
     3001 tggcacgtag cgcccgtccc atagtaactg tggacaaaac taaatccatt cgtttttcca
     3061 gatcaaggtc cctttttgca gaattcgctt caggaaaaaa agccaaatgg acactttggt
     3121 gatctgtgtc atggcgcagt gtccggaaaa tgccgtcggc aatatatgga atgaacgggg
     3181 caattaattt actgaactca aagagcacag tgtaaagtgt agcataagct tcccgcttgt
     3241 cgtgaccctg acctgctttc cagaatcgtc tcctgctgcg gcggatgtac caattggtca
     3301 acaagtcgat gaaacctaca aatggcgcaa cggaacggtt cagatcataa ccgtccattt
     3361 ctttctgtac ctctgcaatc agtttatgta atcgtgaaag aatccagcga tccaagggat
     3421 tactgttttt taccgagaga ttttccggac tcggatccca accgtcgatt ttggcatagg
     3481 tactgagaaa aacgagtgca ttccaaagtg gcaggagttg ctgtcttgtt gtttcaacta
     3541 atcctttttc tgtaaaacgt aaatcttctc cacggactgc cgggctgttg agcatgtaaa
     3601 gacggaccgc gtctgcacca tatttgttca gcatttcatt aggatccgga tagtttttca
     3661 gccttttgct cattttcttt ccatcttcag ccagaataag accattcact acacaattgt
     3721 tgaaagccgg tgtgtcaaat aatgcagatc ctaaaacagc aagtgtgtaa aaccagccac
     3781 gtgtctggtc taatccctca caaataaaat ctgcaggaaa gtttgcttca aagcgttctt
     3841 tattttcaaa cggatagtgt tcctgagcat agggcattga tcctgactca aaccagcaat
     3901 ctaggacttc cggaacacgt ctgtatgttt ttccattttt ttcaataacc aatggatcca
     3961 caaaatgttt gtgcaggtca gtaatttttt ctccggaagc ttcttccagt tcagaaacac
     4021 taccgaagca ccagacatcg ccgtcttcac tcttccaaac tggaagcgga gtaccccaga
     4081 aacggtttcg actgatggcc cagtcacgcg cattatccag ccattttccg aaacgtccgt
     4141 cacgaatgtg ccctggcacc caatgtacag ttttgttgtg agcaaccatt ctgtctttta
     4201 ttgcctcaac atttacaaac catgtagaaa tcggtttata aatcaagggt gtatctgtac
     4261 gccagcaaaa tggatagctg tgttgtgctg tctcatggcg gaacatttta tgttcttctt
     4321 tcaggcgctt gacaatagat ttatcggcat ctttgatatt cattcctgcg ataaattcca
     4381 tgtaatccat aaaattgcct tcgtcatcca ctggatcaaa aatagatata ttttctcgct
     4441 gaaaaatccg tacatcgtct tctccaaaac aggctaaatg tacgatccct gtcccgcttt
     4501 cgtcagtgac ataatcatcc aactgcacag accaggcatt ggtagtatcc actttggctt
     4561 ttgcaaaatc gaagagtggt tcataagtca ttccctttaa ttcagaacct ttcatttcag
     4621 tcagaacttc atagcttcca tcatcaaaat ttgattcgat tcgattctta gtaaggagat
     4681 aacaatcccc acttttttta tctctgattt tacaatatgt tagtttagac cctgcgcaga
     4741 ggctcatatt ggcaggtaat gtccagggag ttgtcgtcca tgcaagaaaa tacaagtttt
     4801 cttctccatt gattttaaag cgtaaagtga cggaagggtc ctgaatttct ttgtaattca
     4861 ggtttgcttc aaaatttgaa agtggagtgg aaaccctcca ggaataaggt accaccttca
     4921 cgccctcata aataagaccc ttttcccaaa gtcgcttaaa aacccaccag accgactcca
     4981 tgaaatcaat gtccatggtt ttgtagtcat tgtcaaaatc aacccagcgt gccatgcgtt
     5041 ccacagtttc gcgccattca ccagtgtagc gtaaaacaat agaacggcac tcctcattaa
     5101 agtttccttc accaaaattt tcaatttcat gccgaccatt taaaccgaga ctctgctcca
     5161 cttcgaattc cacaggtaaa ccatgtgtat cccaaccaaa acgacgttca accctgaagc
     5221 ccctcatggt tttgtaacgt ggaattacat ccttgatggt tcctgctaag aggtgcccat
     5281 aatgtggaag tccggttgca aaaggaggtc cgtcgtaaaa gctgaacggc ttgtcttttg
     5341 gacgttcatc gattgatttt tgaaaaatat ctttttggcg ccagaaattt agaatttctt
     5401 cttccatctg cgcaaagctg agtttgttgg aaacctcttt catttttttg ataaattgta
     5461 gctggattgt cttataaatt tcggtgggcg tcttaaacta aacaaaaaag taaaacaaac
     5521 gttaattcct tgggatttct cttgaaaacc tgacagcaag attgaaataa tccatggcta
     5581 aagtaccaag actttcatct ttttgggcta ctttcagttc acaagttttt acaccctgtt
     5641 ttagtgcaca ataggtttga taaactggaa gcatttttag taaatcttta tcttttgaga
     5701 tttcaagata ctgctttaca aatgaatcta acagctcagt tttccccaat ctgctaagtt
     5761 caactgttaa ggacgaaacg tcatttgcca cgtctagaac tgccaatttc ttctgtattt
     5821 cctgtggact gataaaacga atcacatcgc cgttcaagaa aatgtgttct ggtaaaaaag
     5881 ctccatgtcc cagcacaatc ctgccattgc gcatacgctt ggtgaaaaga cgcttgttgt
     5941 cgtctataaa cttttcaaga ggatgtctga tcatatctaa gatcggctgt gtcagggatg
     6001 cctcaaaata gcgtttcaac tgaaaaagca aatcgtcaca ctgggcccga aaaggctcag
     6061 gttttccggt ttctgcttct ttgtcttttg caggagacat tgcatgaata tctgcaattt
     6121 tggttgcaat gccaaccata ttatttgttg taattttatt tttgtccagc agttgggcga
     6181 gctgccactg atccgggagt gcttccattt ttaaggcata ctcgattgtt gttcctcctg
     6241 ttcctccaaa tttaatgacg ccattatgtt cagtgaccgg aaggacctcc actgcccagt
     6301 ttggattaaa acgttggcta agcgaacact cttcacgaca aaaggcttct ttgacggcta
     6361 atgtcgggta ctcattaccc atttttttaa tcttgtaaag ccagtcatct gccctaaaca
     6421 aatatgatgt tgtagtttcc agaagtttta ttttttccgg gcttttcagc agatatgcag
     6481 ccggtctttt gagggcctca acccattctg gttgtgttac agtcatagtt tattctctaa
     6541 ctaaaattac tttcacagaa atcttccgtg aagaatatca aaaaaataat ttgataaaat
     6601 tgtcaacaag gtcactcaac tcacattcac aaatcatgca taaatatgta gtcatgttac
     6661 tgtatttagg tggaaaacaa ataatcgaca attgagataa atgaattagc agataaattc
     6721 aaatattcag aatgtgaccc agtttatgtt ttttggtatg catgtatttt ttatttccag
     6781 gattggtctg aatttcaagt ggtacccttt ccactacttc cagaccatgc ccctgaactc
     6841 caactatttt acgcggatta ttagtcatca ggcgcatccg gcgaacaccc agcataaaca
     6901 gcatttgtgc acccatacca taatcacgca aatcgtcttt aaaacctaat ttcttatttg
     6961 cttcaacggt atctgctcct tcttcctgga gtttatatgc ctgaagtttg ttgatcaatc
     7021 caattcctcg tccttcctgc ggtaaataaa ggataactcc tttgccctca ctttccacca
     7081 tctccattgc agcatgaagt tgaggtccgc agtcgcatcg gtatgaacca aatacatcac
     7141 cagtcaaaca ttcagaatga acacgtacca gtaccggttc atcctcttcc cattcaccct
     7201 tgacaagggc gacataatca ctgtcatcaa gtacactttg aaaaccaatc actttaaact
     7261 ctccaaattt agatggcagc aaggcttctg cttttttaat aaccagtgtt tcattacgct
     7321 ttctgtattc aatcaggtct ttaattgtaa tgatcagtaa tccatgatga tctgcaaatt
     7381 ttcttagttc tggaacacgg gacattgttc cgtcttcatt catgatttca caaattactc
     7441 caccaggcag catcttggcc atttgtgcaa ggtcaacagc agcttcagta tgtccggccc
     7501 ttcgcagtac tccaccttgg cgagctaaca aagggaagac atgacctgga ctgcgaatgt
     7561 cgtgaactgt ggattcaggg tctattgcag ttttaattgt ccgggcacgg tctgcagcag
     7621 aaattcctgt tgttactcca acagaggcct caatgctgac cgtaaagttg gtgcccattg
     7681 tttcattatt gttgctcacc attaatggca gattcagtct ttctacagtt tctgcatcaa
     7741 gggggaggca gataagtccg cgtccatatt ttgccatgaa attaattatt tccggagtag
     7801 ctgcttcagc agcacaaatc agatcacctt cgttttcacg gtcctcatcg tctactacca
     7861 caattatttc accacgagca atggctttaa tcgcgtcctc aatagaatta aaaaaatcgt
     7921 ctttagttat cattttagct taaattatac aaacgaatat aatatatgat ttgtaataac
     7981 aaatgtttaa tgccaagtac agatgctaac taaatgtgac attccatgaa aattcaggtg
     8041 gcttttctca tgaaccactc agcacagcat aaaccgataa cagattaaag gagcggaatg
     8101 acacgttatt ttattattgc tgaaatcatc cagtcctctc ccagagcagt cactgagctg
     8161 atttccattt gaggagcatc atctacggaa tcgacaccca aatttccgca ccaagacaaa
     8221 gcttcctgtc ctccgattac tttgggtgca agaaataatg caagctgatc tactgtattg
     8281 ctttttataa agctggcgtg aatcaggctt ccaccctcta ccagtagact gttcatacca
     8341 agcttacgta tttccgaaag tacccacaat atgtccggag ttctagtggg agcagttaaa
     8401 atcgttaatt cagctaattc cggccattta cgcaaaggtg catgatttcc agtaacaatt
     8461 acaacaggta ctccgtcctg ttgaaaaaca cggctttgtt ccgggatgcg tccttttgaa
     8521 tccaaaacaa tccggactgg tgatcttcct ccgtctatgc cacgaacggt caattcagga
     8581 tcatctgtaa gcacagtgtt gatacctact aaaacagcat cattttcatt tcttaattcg
     8641 tgaccttttt gccttgctgc ttcacctgta atccattttg aatgaccggt tgcagtggct
     8701 attttaccat caagtgtggc tgctgctttg agagtgacaa tgattccgga gttttttgtc
     8761 atatgtctct ccaactttga gtcactcttc agtcttcagt aaagaatcgc aggtttgtct
     8821 tttgtataaa actatatttc gtcagttaga ttctataatt attagcccaa aacaacaagg
     8881 aatatgaaca tgaacggtag tgacagcagg ttttaattaa aacaatagtt taaacaacaa
     8941 acacagtgta ccagccaatc tggtaaatga tgttttaatt tttgctgtaa aagaagtaat
     9001 tacgtagatg gcagggtact atttgatgtg aggataacag gagcggatac cttgtttttc
     9061 agcccattcg tacatcaact tgccgatatt gagcatgtct ttgggggctc ttggattaaa
     9121 atggcctcct tcaacccatt tttcactttt aggttcagga gcacttttcc acatagggag
     9181 gtacgcttca ggcggaacta aaggatcatt tgtgccgttg ataaattgga ttggtgtctg
     9241 gtaaatatca ggtaaatatg actccatttc tccatatttc aagatattcc ccaggacaaa
     9301 tgacaagata tgtgaaagca gacgtactcc ggagattttt atttttgtgc taaaatctgc
     9361 tgaattttca gttaaatcga aatgtaataa tccttgacta aacaatagtg gttgaattac
     9421 atgctgaaaa tttgtgaatc catagacaac catcaatcct gcaacttttt ccggtacaaa
     9481 actggtaagt atagccggaa atgctgcacc aacactacct ccggccagca ccaccttgtc
     9541 tgtaaaacga gggtcagaac gggttccgcc gtggacataa ttaagcaggg cattaagtgc
     9601 acccagagta tgacgagttt cttcacgaat tttctctgga atttgccacc agtcaagaag
     9661 actccagtct ttccaatgat gtctcaagag aaaagaatga acaggttgtt tcatcaaaat
     9721 tgtgtttcca gtgtatttcc aatttccgcc gcctgccaat aaattttctc cctcttccac
     9781 tcctccaaca ataataagag tgtcacattt ctgaccttct ggtctcgcta tccatgcggt
     9841 tgttgagcgc tctggatctg tgattgattt cagttcccat ttttgggaag acaactcctt
     9901 cactttccat tcaggaacgt ctgctgcttg aaaaggctgc tgcagtcggt aaattttaaa
     9961 ggcagcgacg ctagtgacta ttatgcaggc acagacaatt atcagaaaga cacgatacag
    10021 gactttcata tacttttcat attgaggatt gagagacaat catagaacat ggatgatatc
    10081 cattattcac acattaaatg ttaatacaca tgtcagcaat cggtcttgga ctggatacca
    10141 cctttgatga tacctctgtt gccatcttac ggggaaaacg tgaaatactg tcaaacctta
    10201 ctctttctca atataaggaa cacgaagaat ttggcggcgt tgttcctgaa agagcttcgc
    10261 gcaaacacct ggaagttata catcacataa tcgctggagc attgaaagag gcagatcttg
    10321 atttttccgg aattgattac attgcggttt ccaattatcc aggcttgtta ggttctctgc
    10381 tggttggcct gactgtcgca aaaagcttag cgtacacctt acaatgtccg ttaatcggag
    10441 tcaatcacgt cgaagctcat ccttatgcaa atgttctcaa gcatggagaa atgcagttcc
    10501 ccatcctgca tctggtagtt gcaggagggc atacattgtt gattcatgca agaggacatt
    10561 ttgattataa aataattggt cgaagtgttg acgacgcggc tggtgaatgt gttgataagg
    10621 tagcaaaaat gtttggacat tcaatgcctg gaggaccggt ggttgatcga gctgctatgg
    10681 aatttccagg ggatgtatat gattttccac ggccactgct gcacaaaaaa gctcataatt
    10741 tttctttcag tggtttaaaa acagccatgc tgcgatttaa agagaagtct caatcaactt
    10801 cgtcttcaag ttttgattct ccagatcgtc ttcaagaaga tggtcccgtc ctcgcttctt
    10861 tttttcaaag tgtaattgat gtgttaattc acaaaacttt caatgcagct gaagcttacg
    10921 ggcttaacaa tatttctgtt tcaggtggtc tggctgcaag tcgaaagtta cgtttggctt
    10981 ttgaagctga atcagccagg aaaggagtta agctttttta tccgccgcca aatctctgta
    11041 cagataatgc ggcaatggta acctgtctgg ccgctcatcg ttatgaggca gaactttttg
    11101 atgatcttac tctggatgca tttgccaatt tgaattgagg aacatcctga tttaattctt
    11161 tctgaaaatc aaagtgctgg cccaagagag ccctacacca aagccggcca aaactgcaac
    11221 ctgcatttct tgattggcat attcctgggc gataagcatc ggtacagatg aggagactgt
    11281 gtttccatag gctgcagaat gaaaagggca gcgctctcgc ggtacaccca gattgtcggc
    11341 gatggaattt actatgtgta gacttccctg atggagaagg aaacggttta cattttcaag
    11401 aacaaaagag tttttctcca gcatctcccg gatactgtcc ggaattatgc gtactgtaaa
    11461 agtgaaaact gcgcgtccgt tcatatgaac gtaaccgtct tcacgaacaa tgataccttc
    11521 ctttttgctg ccgtcagagc caaaaacaaa ttgcccgaat tcaaagacag gttcatctgt
    11581 cagcaaagta acagtggcac catctccaaa gagtgttgcc gttttcttat cttcctgatt
    11641 cattactttt gaataaggat ctgcagtgaa gagcagtcct cttcggaaat tgttggcctc
    11701 catgaaagat ttgatcacgg aaagtccgta aacatatccg gaacatccaa gagagagatc
    11761 gaaaacagcg caatttaatg ggaagttcag cttatcgtgt ataattgctg aagtatgagg
    11821 gagaccatga ccatccggat tttgagtaca aacgataaga caatcaattt cgtttggcga
    11881 aacaggatgt tgctgaagga gattttccca ggatttgaca cacagatccg aagttttctg
    11941 ctctggagct ttaagagcta cttcggtgaa gccgattttt tcccgtataa aagattcggt
    12001 catgtcgaac ttctctatac ggtcaaaatt tgatattctt ttttccggca aataggttcc
    12061 aattgccttg atgcctatca taaccgctac ttttctttgc taaaaggttg tccaattgct
    12121 tttggtgcaa cggcttttcc aacaaatcct gccagaaaaa ttattgtgag aatataagga
    12181 gacatatcca aaaaactatt cagaagttta accggtatga tcccctttag caggtcaaaa
    12241 atttcaggtt tgtccttgat tgcaaacaaa aaagaaaaaa gcaggcaagc aaacattgca
    12301 ggataaggac gccatttacc aacaatcatt gcagccagtg caatataacc ttttcctgca
    12361 gacatatctt taacgaaaaa agccaaatgt gctgtagaaa gatatgctcc agacattccc
    12421 cctaatattc cgttcaggat taaagcctga taacgagttt tgttgactga aattccagta
    12481 gtatcaacag ctaagggatt ctctccggta gctcttaaac gtaaaccgaa actactttta
    12541 aatacaaccc atgcggtaat tggtactgct ataaaggcta tgtaaaccag agcattgtgg
    12601 ccgctgatca gttcataata aatttctcct atgataggaa tgttacggaa ctcttctgca
    12661 aagggcaatt caatggacat aaaacgggct tctttgggaa gttgtggagt ttttcctccc
    12721 atgtgaaacc aggcgttggc aagaaccaca gtcaaaccca ttgcaaaaat atttatagct
    12781 acgcaggaaa caacttgatt ccctcttttg ctgacactgg cataaccgtg gatcattgaa
    12841 aaaataattc caatcccgat agatcccagg agtccccacc aggcagattg agtcaaatgt
    12901 gctacagttg cagctacaaa ggcaccaccg agcattttcc cttcaagtcc aaaatcaatg
    12961 acgcctgaac gctctgcaaa aataccaccc attgcggcta aaacaaaggg cacagaaatc
    13021 ctgagagtgg catctagaat taatagtaca tcagtccaca tatgatggtt aaatgttgac
    13081 ggttagttaa ctgctcttta gcaaaatgaa tgcggaggct ctatatcgac agccgccgaa
    13141 atatatattt actggcgtgc cagctgtttt acaaaatact caatttgagg ccggaaaagg
    13201 ttttccagcg ctccggcaaa taaaataacc aaaccctgga ttaatacaac caaatgacgg
    13261 tcaatagctt gtacttcaaa tgacagctct gcaccaccct gataaagaat tccaaacaac
    13321 agggcagcca gcaaaatccc taatggatgg ttgcggccaa tcagtgaaac tgctattccg
    13381 gtaaatccga taccggcctg gaaattaagc atgattctgt gactggagcc cataatttcg
    13441 tttataccca caaatcctgc caatccacca caaacaacca tagccagaat aatattacgg
    13501 gaaacgctga ttcctgcgta aacggctgca gtatcatttg taccaactgt ccgaatttca
    13561 tagccccagc gggtgtacca aataaatcgc caaaccagca ccaggcagat tagcgcaaag
    13621 aagaaagtcc catttagtgg agtctgtgta atattaattc caaaccagtc aaatatttca
    13681 tgcatttgcg gaatccaaac cccctctgca aattcacggg attcaggtga catctctttg
    13741 ggaggtctta agacatgtga caagatgttc accatgataa ctgaagcgat aaagttgaac
    13801 atgatggttg tgactacctc atggctaccg cgtttagctt taaaataacc aggaattgca
    13861 ccccaaattc cacctaatat aatcgaagtg agtactgcta atggaaagac cagccaaaga
    13921 gaccaatctt ccagccagag acaaatcaga cctactccaa gtccaccaat ataagcctgg
    13981 ccttctgcac caatattaaa caaaccgcaa tggaatccta tcgcaaaagc caaaccggtg
    14041 aaaataaaat tggtagtgta ataaagtgta tagcccaagc cttcgtcata tccaaaggca
    14101 ccataaagca gggtttttag tgcaaaccag gggtcttctc ccagtgccca aatcacaaca
    14161 ccagagataa aaaacgcaat taaaaggttg gtgacagtaa ctaacgtaac caccagggtc
    14221 tcagtcctac gttcgttcat gccgcttcct ttcgtactcc ggccatcaac agtccaattt
    14281 cacgttcgtc agcatctttt gccagaattt ctcccatgat ttgtccgtca aacatgacca
    14341 aaatccggtc actcaaagaa agaatttcat caagttctac agaaaccagt aaaacagctt
    14401 ttcctgcatc acgcatagca atgatttgcc gatgaataaa ttcaattgct ccgatatcga
    14461 ccccccttgt tggttgaccg accaccaata atgcagggtc acgctcaaat tctcttgcaa
    14521 ccacaagttt ttgttgatta ccacctgaaa aaagagaagc tcttaaatga ggattgtccg
    14581 ggcgtacatc gaaatgctcc atctgttttt tgcaggactg tatgatgaaa tctctatcca
    14641 tttcaataaa attgttatag gaaggatttt tgtggtagcc caaaataata ttttcgtttg
    14701 cagaaaatga agtgaccaag cccctgcgct ggcggtcttc aggaacatgg gccattccaa
    14761 atcgtctcat tgttgcagca tctgctctaa cggttgaagt gatagtttgg ccactgatct
    14821 ggatttggcc tttttgaaat ggtttgattc cagtgattac ttccatcagt tctgactgac
    14881 catttccgga gacgccagca attccaacaa tttcaccaca atggacctca aagctgacat
    14941 cttttacacg gcgaacaccg aaatcatcta tataatccag gtgttttacg gtcaacatag
    15001 gacttccggg ttttgctggt gctttatcga cttgcatgag gacttttcgc cctaccatca
    15061 attctgctaa ttcttctctg tctgtttcgg aagttttgcg gtgtgcaacc atttcacctt
    15121 gacgcatcac ggaaacatta tccgtaatgg ccatgatctc acggagtttg tgggtaatta
    15181 atattactgt tttgccctgt tcacgaagct cattgagaat gcgaaataaa tgatccgctt
    15241 cttgtggtgt caagacacca gtgggctcat cgagaattag aatatcagct ccacgataga
    15301 gtgctttgag aatttctaca cgctgttgaa gtccaactgg taaagtgtta ataacagcat
    15361 ttatatctac ttccaaatga taatctttct ccagacgctg aagttctcgt tctgtttttt
    15421 gcattgattc ctgcagagaa aatccagttt cagtgccaag gacaacattt tcaagtacag
    15481 tgaaagtatc taccagcatg aaatgctggt gtaccatgcc gattcctgca gcaattgcat
    15541 catgggcact acgaatttcc gtagattttt cctgtacggc aattgcacct gaatctgctt
    15601 gataaaaacc ataaagtata ctcatcaaag tagattttcc tgcaccattt tcaccaataa
    15661 taccgtgaat ggttccggac tcaaccttga gcgaaacgtc tttgttagcg cagacgttgc
    15721 caaaatattt attaatatca gttagttcaa tggctacagt agacattttt tttgtggctg
    15781 cgtttgttta taattgagtg ttggtgccgt gtcttgtttc ctgaaacata caagggtact
    15841 tgtttttaaa gtatggcgag acgtttcaaa atcaattgga aaaacccatc tgcgttaatt
    15901 tgattgatat aagttacgtt tggcggacgg tcagtaacct tccaccagtc gaccaccgtc
    15961 atacccattg tgagttcgga actaatttcg atttcaacat tgacaaactt tccctcaaaa
    16021 agttcctgtt ggagcaaata tgcaatcact gtaggatcat gtagagggcc acccccggaa
    16081 ccatattttt cgagatcaaa tctctcatag aagctcatca taccgaaagc agcatcaccg
    16141 actggagttc ccagagcttt caagtcttca agccatttac gctgcatcag tgctttgtgt
    16201 gttacatcca gtggcagcat aacaagtgga cggccacagc ttaaaactgt actggcggca
    16261 tgtggatcta cgaagatatt aaattcggaa gtgggggtga tatttccgcc ttcaaaatat
    16321 cctcctccca tcattacgat atttttaatt tttggcagga tttggggagc tttaatcatg
    16381 gccattgcta catttgtcaa aggcgcaaag cagcaaatcg tgattgaatt gtcttcagct
    16441 tccagtaagg tctcaatggt ccagtccact ccgtgctgct tttgaaggga cattgttggt
    16501 ggtggaagct ctgcaccgtc caaccctgtt ttcccatgca catgctctgc tgtgaccaaa
    16561 ggccgtacca gcggtcgtga acatccagca aatactttag tttccgtttt gtttgcgagc
    16621 tcacatagaa tcaacgcatt tctggaagtt aatgataatg gaacgtttcc ggcaactgtg
    16681 acaattccga gaacatcaag ctctgcagga gaagccagtg ccagcaagat accaatagca
    16741 tcatcctgac caggatctgt atcaatgatt attttttgag agggcatgaa aaaagagttg
    16801 ggtgccgggt aaagtgggta gaggggtgtc gggtgatgaa aaacatcacc ctaaacactt
    16861 aaataattta gtcacccgtc tttatttaga ctactgaacg gggcatttat tgtcactcat
    16921 gtagtcatgt attttgatct ttcccgatag gatcccttcg cggacttgct ccactttgga
    16981 ttccatagca ctggttacca atttggcatt gtgctcatca agtgcccaac ccacacca
//
LOCUS       DTVD01000246           17016 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_623_length_17016_cov_487.605684, whole genome
            shotgun sequence.
ACCESSION   DTVD01000246 DTVD01000000
VERSION     DTVD01000246.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 17016)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17016)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17016
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_17016_cov_487.605684"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(357..2000)
                     /locus_tag="EYO68_09150"
     CDS             complement(357..2000)
                     /locus_tag="EYO68_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08003.1"
                     /translation="MHRFTKIFVTVLILGLISSALYASKGSNSTPFPVPLSCYSEDYG
                     SPNFLAERCDQVHGSEGFRDPEGASMAEILSHRISANPFNLVVSLIFLIAILHTFMAN
                     KLTAKAHQIHEEHDERMKAAGASEEEIKHDIPFKAELFHFLGEVEVVFGMWVIALLFV
                     TIGFFDWTTFKNYMVYDRVFIEPMFVVVIMAIASTRPVVKVSEQLLGLAAGLGGHSKA
                     AWWFSILLIAPLLGSFITEPAAITIAALLLANQFYKHRPSSGFAYATIGLLFVNISVG
                     GTLTHFAAPPVLMVATTWDWSMGFMAANFGWKAALGILISNVLYFIVFRGQFAKMGKD
                     EVKEASAEFHTPEVQKLKPGQMSHDEFEAMWAERETTIPWWVTLVHICFLAWTVYTAH
                     YPALFIPGLLFFLGFMSLTATHQNKMELKGPVMVGFFLGGLIVHGGLQAWWIAPVLGS
                     LAEVPLMLTATILTAFNDNAAITYLATLVPNLAEASKYAVVAGAVTGGGLTVIANAPN
                     PAGQSILGRFFEHGVNPLKLLIAALIPTIIMGICFMVLP"
     gene            complement(2289..3869)
                     /locus_tag="EYO68_09155"
     CDS             complement(2289..3869)
                     /locus_tag="EYO68_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08004.1"
                     /translation="MNQIRKYWLGLIMIGGLAVIAVAGGDVGSSPLFPIPLDSYGDAD
                     LIKSGDIFAVLGNRITHTPFNLFASLIFLFAIVHTFAAIKITGIAKKMELTHAEQMRK
                     SGKSEEEIEQDPPVMAEMLHFFGEVEAIFGIWVIVLAAVTISFYDWSTFKIYIAQTVN
                     YTEPMFVVVIMALAATRPVMQLAKQILGKFASIGKSSPGAWWLSILTLAPILGSFITE
                     PAAMTIAAMLLAEQFYRLKPSSKLAYATIGLLFVNISVGGTLTNFAAPPVLMVKTPWD
                     WTSSFMTLNFGWKALLGILISNLIYYFAFKKEFSKFASASEAEVEMEKSIENNEDSVP
                     IWITIVHLLFMGWTVFNAHYPPLFIGGFLFFLGFAMATKAHQSHINLKSPLLVGFFLA
                     GLVTHGGVQAWWIAPVLGSLGDLPLMLMATVLTAFNDNAAITYLSTLVPDFAISAKYA
                     VVAGAVTGGGLTVIANAPNPAGQSILSKFFPGGVNPAKLALGAIIPTVIVGLAFMAIP
                     QDVHDPQSVDQSKVEISD"
     gene            4089..4985
                     /locus_tag="EYO68_09160"
     CDS             4089..4985
                     /locus_tag="EYO68_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08005.1"
                     /translation="MLKRQKEKRQKKMIRRRKAMPKKNSQQAGSPQQLEQLLNTLPTL
                     AFEPELTDLNMGEEKLRTLLESEQTEIEIILELLTEEFIADLDQRLEQLESAHSEKSI
                     KSVLAKATRHQIANSDKIPHLSNPVLIALFLKTRSAVEGEKLDLNSLPAAMEEFDKRN
                     HDFIQELTKQMEGSEKDDSILEADGELLDEEKPEERTPAIETDVYNKFMELVPAEKQE
                     QVEEDLEVFLVDFEPPPISEWDSELIKNFLEKWFLENANPLEEDLVSMRESLLSLFKF
                     LAKEDLLPDGFLNNVSKSIQNS"
     gene            5053..6516
                     /locus_tag="EYO68_09165"
     CDS             5053..6516
                     /locus_tag="EYO68_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIC08006.1"
                     /translation="MSKQEIIEQSKESSQILVLDDEHSIRWVLERTLSQAGYIPHLAA
                     DVLEARPLLQKHPIRLAFVDINLPGKDGLSFTREILKEFPSLLTIVMTGQSTMYNTVE
                     AMKAGAFDYVAKPFNIDEIEELVKRALRVPLSTPETKRIKSGKKADDLLAGQSKVMRD
                     LYKAIGRVAATDLTVLIQGESGTGKELIARSIHQHSLRSSQPFVAINCAAIPSELMES
                     ELFGHEKGAFTGATERKRGKLELASQGTLFLDEIGDMPMRLQSKLLRVLQEKQFERVG
                     GHELITTNMRVIAATHQNLEDLIKNSNFRTDLYYRLNVFPLNIAPLRERREDIPVLTE
                     HFLQKGSKEMAVGRKAVEPEVMRVFALYNWPGNIRELENTVKSLMITNVTGTIMLDSL
                     PKNLFRPQPVTDSGESLEEVASLKLEGLVRDAVEFDRDSLLAEVLPQVERPLLKLLLE
                     QTNWNQQKSARILGINRNTLRKKIETLGLRNQRDIEK"
     gene            6513..6839
                     /locus_tag="EYO68_09170"
     CDS             6513..6839
                     /locus_tag="EYO68_09170"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIC08007.1"
                     /translation="MKTGTSATMFQRVYDVILERKNYSPDNSYVASLMDKGIDSILKK
                     IGEESAEVILATKNENRKEQIHEITDLWFHLLILMGYQGITIEDISQELKKRFGQSGL
                     EEKVQR"
     gene            6870..7904
                     /locus_tag="EYO68_09175"
     CDS             6870..7904
                     /locus_tag="EYO68_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIC08008.1"
                     /translation="MPSVSKSSSISTKIAQSKIRAVFQPFKVQGIQFRNRILAPPTVD
                     DHADVHGKVTLAIIKHYRSLARQGAGTIIVESAYVTKQGRSHVNQLGISDEEHLEGLE
                     KLAQGVKKEGATIGIRLSHAGAQTSESACGEQPVGPSVLNFGRDFDISREFDQGDVEE
                     IVLNFVHAAERAEEVGMDFVEINGTEQQLLDQCCCIKLNNRDDEYGTAKIENRVQLAL
                     DVVNSIKKRESVKIPLSYYFSIFDKIDDGFKPKDLKKMLTLLENAGVDIFHPLAIHAM
                     NKCFENKEPLCHWSAKYTDKPMIINGNIKSPQLLEEAATLGCADWFAMDQSIFDRLQW
                     YQFLKRKIDK"
     gene            7925..8317
                     /locus_tag="EYO68_09180"
     CDS             7925..8317
                     /locus_tag="EYO68_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIC08009.1"
                     /translation="MSRSIIKTSKAPAPVGPYSQAVSCGNILYVSGQIPLDPESGELV
                     GNSFAEQCHRVLLNLKIILEAGGSGLERVLKVTIYMKNLAQFGELNEIYSEYFDVSKP
                     ARACVEVSNLPKGADVEMDAIAEISSVN"
     gene            8347..9636
                     /locus_tag="EYO68_09185"
     CDS             8347..9636
                     /locus_tag="EYO68_09185"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIC08010.1"
                     /translation="MSALNIAIAGFGTIGSGVAHVLEQHSKEPDATINLVGILESDTK
                     GKFARPWFEKRPELFYDNPDKLLADSQVNVIVETIGGSGFSRELITKALKNGKHVVTA
                     NKDLIAVHGAELMAVARENGRHLLFEAAVAGAIPVLRLLKDYLQVQDIQGIRAILNGT
                     TNYILSEMECNDLSFETALSQAQELGFAEQDPTNDIKGIDARYKLVILTYLITGQWFA
                     PEQINLEGIDHLELADFEYAERMGRSIKLIANLRLSDQGVQVYVLPLMIEKDDMLAKI
                     TGSTNAVTLSGKYADDITLIGKGAGSLPTASAIVSDLNKIQRHPEPFPPPPSNKKLEI
                     ESMDNIQFRHTLRFEVRDQPGIFGHYGSIFERHSINIYALEQLPQYHRIGKEGEETVI
                     FTLTLQNCKEGLVHKALKEINQADYMLKPVVLLREIV"
     gene            9696..10532
                     /gene="mreC"
                     /locus_tag="EYO68_09190"
     CDS             9696..10532
                     /gene="mreC"
                     /locus_tag="EYO68_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIC08011.1"
                     /translation="MFKFIGTYRLYISIVATLIVLFFLMYQTYNEQGTSRFQMAVQTI
                     TYPVQTSVQSVVSTIKNTWNSYINLIEVNEENKLLKQQLLNMEEKLNQHIENSVQFFR
                     LRSQLLFAQKKAHKEIFSEIIGESADNTHQIRLINRGSNQLLQRNYIVIRKEGLVGRI
                     QSVSPYQSSVQLIIDHRSRVPALIQRNRVRGLIYGTHDGMEMRQINQHAKIKIGDRVI
                     SSGLGNLYPKGILIGWVSGINHEPHELFKTARLDSAVDFNQIEEVFTILPSKSDSNLS
                     VE"
     gene            10529..11029
                     /gene="mreD"
                     /locus_tag="EYO68_09195"
     CDS             10529..11029
                     /gene="mreD"
                     /locus_tag="EYO68_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIC08012.1"
                     /translation="MMRLFMLSLGFLLIGVLMNIVFNYYFLPVGLKINWMLIFILVLT
                     FRHSSISLSFLGVFAGMILDSLSHGYIGLYGISFFVTLLLARQLIKLFYANTFFAVSL
                     AVSVMTILEGWISLSILGMLETELNQSSLMLTSTLPLAVLHGLVTPFILQSVIWGENH
                     FIGDTA"
     gene            11026..12912
                     /gene="mrdA"
                     /locus_tag="EYO68_09200"
     CDS             11026..12912
                     /gene="mrdA"
                     /locus_tag="EYO68_09200"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIC08013.1"
                     /translation="MKLNPLDFEELDRIRIRLIILGGVVILIFSLLASRLWYLQISQG
                     EKYLEFSQGNRIRLVPEPALRGNIYDRNGVLLAENRPAYQLLLIREDTPNLETTLHKV
                     SESLKISYSALQEKIDANQDLAQFRPIILEEDLEYEKAMHLETFQDDFPGVSVVVRPS
                     RFYPQKNFASHLIGYVGIVQEDWSELPAEKRSSSQIVGHSGVELLENDHMIGLDGGRQ
                     AEVDHMGRELQVLGEPVPSVPGKNITLSLDVRLQKVATDAMKGESGAVIVMNPKTGGI
                     LALASFPDFDPNLFSGGIDPENWKRLLENPQHPMENKAIQGLYAPGSIFKVVTAYAGL
                     EKGAITPETEYVCNGFFHVKGRSEPFKCWKETGHGRVKLHDAIKGSCNVFFYNTGMGA
                     GVDELHKFASLFRLGKLTGVGLLNEKAGLIPSSSWKKRVFNEPWYGGETPSMSIGQGF
                     IIVTPLQVINMINILANDGMWVPPRLFLEDEGTAPQQIPLNKEYLSLIRKGMIAVVNE
                     AGGTARKVQFEEFTVAGKTATSQVISHKTMETLDEEAKLKKEFQNHAWFVAFGPEEDP
                     EISVIALVEHGGGGSRVAAPVVRKILSYYIDNIYKPKSKKTVQTNFESKNFKFSTRLQ
                     MAFN"
     gene            12995..13630
                     /gene="maf"
                     /locus_tag="EYO68_09205"
     CDS             12995..13630
                     /gene="maf"
                     /locus_tag="EYO68_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HIC08014.1"
                     /translation="MSQFIQHRPLCLASSSPRRKMFLKKYGLQFDCHTPIINETPEKS
                     ESAKSFVKRMAHEKALQVQRTYSKLAEDVIILAGDTIVYFDGKILGKPQSIQDARTML
                     TQFSGRTHQVFSGYSILDSSSGNHTTDLVCTEVRFLKLNADLLEWYLNSKESFDKAGA
                     YSIQGLGAILVESISGSYSNVVGFPIECIIPNLTANGWISFAENTQHEVYG"
     gene            13627..14601
                     /locus_tag="EYO68_09210"
     CDS             13627..14601
                     /locus_tag="EYO68_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08015.1"
                     /translation="MKYYLFILVAVFLIPTPTHAIEFENRLPESVWEVEMRLQHTPVY
                     DRAFNGYGEEAPLQQLMLWDREWRDSVVGKLQREEQRLEIRMAYGLTEKWMLEATIPL
                     LQKKQTSTLKIESATAIQQKVLENLASENLSGLGDIKLIFAKDIVTTTTWHNRGGFTL
                     RLPTGTTGTPRGISTNSTGEGHGSVGAYFHFNWYPLTHGIRNAFRIEGTNELVGKRET
                     LDGEKGYYSAGHRVDLFYNWSIERQNIFAGTELHYYQQAESSLPTGKSNAAFLKEINF
                     EFGYGNLSELEQKPLSTPWQVRLGYTRPLAGQNTPIANRWELSSTFFF"
     gene            14660..15166
                     /gene="uraD"
                     /locus_tag="EYO68_09215"
     CDS             14660..15166
                     /gene="uraD"
                     /locus_tag="EYO68_09215"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIC08016.1"
                     /translation="MNLEDLNHCDIKQAHIELLKCCGSSQWTEKMLAARPYKSVSHLL
                     ELAGQIWSDLGEVDYLEAFAAHPKIGASKPPDNAKNTESWTSKEQAGMMSADEQTKLK
                     LKTENQKYAEKFGYIFIVCATGKSASEMLELLRIRLENSPETELKIAAGEQMKITNLR
                     LNKMLRDA"
     gene            complement(15203..15547)
                     /gene="uraH"
                     /locus_tag="EYO68_09220"
     CDS             complement(15203..15547)
                     /gene="uraH"
                     /locus_tag="EYO68_09220"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIC08017.1"
                     /translation="MSPITTHVLDTSQGCPGANIRIRLEQQQTDKTWQEIASGSTNSD
                     GRIANLIQEDEMMNSGVYRMLFETGPYFEQQGTAYFYPEIEVRFLLNHPEQHYHIPLL
                     LSPFGYSTYRGS"
     gene            complement(15592..16797)
                     /locus_tag="EYO68_09225"
     CDS             complement(15592..16797)
                     /locus_tag="EYO68_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08018.1"
                     /translation="MDSYIIEWLNLVVRWIHVITGIAWIGASFFFNWLDSQLTPPENQ
                     EPKVEGELWMIHSGGFYQLEKTMISPSEIPRVLHWFKWEAYSTWISGIFLLGIVYYTG
                     SGVYLIDSEIADLSYGMAVSISLGTILASWLVYDFIWASALGEKGWFPVMISFLLLFG
                     IIWWFHQWFGSRAAYIHVGAVMGTLMVGNVWRRIIPSQTKLVEAVKAGETPEASLGIK
                     AKQRSLHNNYMTLPVVFIMISNHFPNTFAHYFNWAILIAIIVIGATVRHFFNLKNKGH
                     LNIWILPVAMAAIFALIYVTKPDATISNSPDTVTFGTEHIPYALVRTILDQRCVSCHS
                     SRPTDDVFRIAPKGIMFDNNERVHALATLIKIQSVTTIAMPLGNKTGMTHEERVILGR
                     WVDEGASIK"
     gene            16892..>17016
                     /locus_tag="EYO68_09230"
     CDS             16892..>17016
                     /locus_tag="EYO68_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HIC08019.1"
                     /translation="MENYPRNMLGYGQKPPHPEWPGKSRIAVQFVINYEEGGENC"
BASE COUNT         5319 a         3506 c         3635 g         4556 t
ORIGIN      
        1 ggaactctgg aagcttggat ttgagggcaa acatgatcca ctccaggaag atgggggtct
       61 ttttttgcgc tggggtattt caaaaaacct gctgaaaacc cagggatctc ttggcgagtt
      121 aaaaggaaat ggaggatatt tagggatcgg ctgggagttt ccttttgaaa ttctggggct
      181 ggcttttgag attgctcaaa gacaaatcag cctggaaaat aatttattca ttaagacctc
      241 cagtccgtct ataggcgtgc acttttacaa gcacctttaa gcaaagtaaa aataatctga
      301 tgattctcta aagcagtgcg ggaattaatc ccgcacgatg tgcctgaaaa tcctttttat
      361 ggaagtacca tgaaacaaat tcccataatg atcgtaggaa ttagagcagc aatcaacaat
      421 tttaaaggat ttacaccatg ctcaaaaaag cgtccaagaa tagactgacc cgcgggattc
      481 ggtgcatttg caatcactgt tagtccacct ccggttactg ctccggctac aaccgcatat
      541 tttgaggctt cggccaggtt cggtaccaat gtagccaaat aagttatcgc tgcattatca
      601 ttgaatgcag tcaagatcgt cgcagtaagc ataaggggaa cttcagccag agagcccaga
      661 acgggtgcaa tccaccaggc ctgcagtcct ccgtgaacaa tcaggccgcc caggaaaaaa
      721 ccaaccatga ccggcccctt gagttccatc ttgttctgat gcgttgcagt gagcgacata
      781 aaccccagaa aaaagagcaa cccaggaata aacaatgctg gataatgtgc agtataaact
      841 gtccaggcca aaaaacaaat atgaaccagc gtaacccacc atggaattgt agtttctctt
      901 tcagcccaca tggcttcaaa ttcgtcatgg ctcatttgac caggtttaag tttttgcact
      961 tcaggtgtgt ggaattccgc gcttgcttcc tttacttcat ccttacccat tttggcaaac
     1021 tgtccacgaa agacaatgaa gtaaagcaca ttagatatta aaatacccaa cgccgccttc
     1081 caaccgaagt tagccgccat gaaacccatg ctccaatccc atgtagttgc aaccatcaga
     1141 actggtggtg cggcaaagtg agtgagcgtt ccaccaactg aaatgttgac gaagagcagg
     1201 ccaatcgtgg cataggcaaa accggaggaa ggcctgtgct tgtaaaattg gttagccagc
     1261 aaaagggcgg caattgttat tgcagcaggt tcagtgatga atgaaccaag caatggagca
     1321 atcagcaaaa tcgaaaacca ccaggcagct ttactgtgtc ctcctaaacc cgcagcgaga
     1381 cccagtaact gttctgaaac cttgacaacc ggtctcgttg aggcaattgc catgattaca
     1441 accacaaaca ttggctcaat gaaaactcgg tcgtagacca tgtaattctt aaatgtagtc
     1501 caatcgaaaa aaccgattgt gacaaacaac aaggcaatga cccacattcc aaacacaact
     1561 tcaacttctc ccagaaagtg aaaaagctct gctttaaaag gaatgtcatg cttgatttct
     1621 tcttcagaag caccagccgc tttcatccgc tcatcatgct cttcatgaat ctggtgtgct
     1681 ttggccgtca acttgttggc cataaatgtg tgcagaatgg caatcagaaa aatcaatgat
     1741 acaactaaat tgaacggatt tgcactaatg cggtgggata gtatctcagc catacttgct
     1801 ccttccggat cccggaagcc ttcagatccg tgaacctgat cacacctctc cgctaaaaaa
     1861 ttcggggaac cataatcttc agagtagcag ctcagaggca caggaaaggg ggttgaatta
     1921 cttcctttcg atgcatataa agctgaactg attaagccca atattaaaac tgttacgaat
     1981 atttttgtaa atctatgcat agtcttctcc gttaaagggt ttgcacattt taatttagta
     2041 cttgaaaaag ctgaatcact ttcccatttt gacaattaag agaaatctcg acaaattatt
     2101 ttttctgtaa agtaaagatc aaccattttc aacatctcaa agtggaaaaa tatttttcct
     2161 gaaagttctt ttacagaagt tattgaaaaa atacaacatt taattaacta cagataaatc
     2221 agattccgct ctgataactc ctgaagaatg tgtttcaaac ttcaaaatat ttaacctgag
     2281 tgttagagtc agtcagagat ttcaactttg ctttgatcca ctgactgagg atcgtgaaca
     2341 tcctgcggaa tggccataaa agccagcccc acaatgaccg tgggaatgat tgctcccaat
     2401 gcaagctttg caggattcac tccaccgggg aaaaacttgg ataaaatgga ttgacctgcg
     2461 ggattaggag cgtttgcaat caccgttaat ccgcctccag tcacagctcc ggcaaccacc
     2521 gcatattttg ctgaaatagc aaagtccggg accaaagtgc tcaagtatgt aattgccgca
     2581 ttatcattga atgctgtcag cacagtagcc atcagcatca gcggcaagtc acccagggag
     2641 cccaaaacag gagcaatcca ccaagcctga actccaccgt gtgttaccaa gcccgccagg
     2701 aaaaaaccga ccagtaaagg tgatttcaaa ttgatatggc tttgatgtgc tttggttgcc
     2761 attgcaaaac cgaggaagaa caaaaatccc cctatgaaaa gcgggggata gtgtgcatta
     2821 aacaccgtcc agcccatgaa aagaaggtga actatggtga tccatattgg gacagaatct
     2881 tcattatttt ctatgctctt ttccatttct acttctgctt cagaagcgga agcaaacttg
     2941 gaaaactctt ttttaaacgc aaaataataa attaagtttg aaatgagaat tcccaataat
     3001 gctttccagc caaagttaag ggtcatgaat gaagatgtcc agtcccaggg tgttttcacc
     3061 atcagtacag gtggggcggc aaaattggta agagtaccac cgaccgaaat gttgacaaag
     3121 aggagcccga tagtagcgta ggctagttta ctggaaggtt taagtcggta aaactgttca
     3181 gccaaaagca ttgcggctat ggtcatagca gccggctctg taatgaaaga acccaagatt
     3241 ggagccaaag ttaaaataga cagccaccac gcacccgggg aactttttcc aatggaggca
     3301 aatttcccta gaatctgttt agccaactgc attaccggac gtgttgcagc caaggccata
     3361 atgaccacta caaacattgg ttcagtgtaa tttactgttt gtgctatata aattttgaaa
     3421 gtggaccagt cataaaacga aattgttact gctgccaaaa cgataaccca aattccgaaa
     3481 attgcttcca cctctccaaa aaaatgcagc atttcagcca taactggagg atcttgttct
     3541 atttcttcct cggatttacc cgactttctc atttgttcgg catgagtgag ttccattttt
     3601 tttgcaatgc cggtgatttt tattgcagca aatgtatgca caatagcaaa caaaaaaatg
     3661 agtgatgcaa acagattgaa cggtgtatgt gtaatacggt tccccagaac agcgaaaata
     3721 tctccacttt taatcaagtc tgcatctcca taagaatcga gtggaatggg gaatagaggt
     3781 gaacttccaa cgtcaccacc tgcaacggca attaccgcta atccaccaat cattattagt
     3841 cccagccaat attttcgaat ctgattcatt aaactaacct gcaattagaa aaaaatttgt
     3901 acttcactgt tgaaagcaaa tgtattgttt tttgatcctg tcaaattaaa caaggaacga
     3961 gcaaataaca agccactaat ttgacgtgat tttatttgat aattctgcaa taatgcgtga
     4021 ttgatttctc aaattgaata cttgtctatt taaatatggc taaaaaaaga aaacaaaaaa
     4081 aaacagggat gctaaagcgt caaaaggaaa agcgtcaaaa gaaaatgatc cggcgccgca
     4141 aagcaatgcc caagaaaaac tctcaacaag caggctctcc acaacaactt gaacaactcc
     4201 tgaatacttt acccacgctg gcatttgaac cggaactgac ggatttaaat atgggtgagg
     4261 agaaactccg tacattgctg gaaagtgaac aaacagaaat agaaataata ttggaactgc
     4321 tgactgaaga gtttattgca gatcttgacc agagacttga acagttggaa tcagcacatt
     4381 ctgaaaaatc aataaaatct gttttggcaa aagcaacacg tcatcaaatt gcgaacagtg
     4441 acaaaatacc acacctttct aacccagtac taattgcact tttcctgaaa acacgttcag
     4501 cagtagaagg agaaaaactg gatctaaaca gtttgcctgc agcaatggaa gagtttgata
     4561 aacgcaatca tgattttatt caggagctta ccaaacaaat ggaaggttca gaaaaagacg
     4621 attctatttt agaagcagat ggagaactgc tggatgaaga aaaaccggaa gaacggacac
     4681 cagcaatcga aacagatgtt tataataaat ttatggaact agtacctgct gagaaacaag
     4741 agcaggtgga agaggatttg gaggttttcc tggttgattt tgagccacca cccatttcag
     4801 aatgggactc ggagttgatc aaaaatttcc tggaaaaatg gttcctggaa aacgctaacc
     4861 ctctggaaga agatttagtg agtatgcggg aatcattgtt gagcctgttt aagttcctag
     4921 caaaagagga tttactaccg gatggtttcc tgaataacgt ctccaagagt atacaaaatt
     4981 cctgaaggac tttttccgcc ggcaggacaa attgttatcc gtgcttggat tcaaaaaaga
     5041 aaaaaataaa ctatgtccaa acaggagata attgaacaaa gcaaagaatc atctcaaata
     5101 ttggtcttgg atgatgagca tagtatccgc tgggttctgg aacgcacttt atcacaagcc
     5161 ggctatattc ctcatctggc tgcggacgtt ctggaagcaa gaccactcct ccagaaacat
     5221 ccgatccgtc tcgcttttgt tgacattaac ctgcccggaa aagacggtct ttcattcacc
     5281 cgggaaattt taaaagaatt tccgtctctg ttgactatcg tcatgaccgg ccagagtacg
     5341 atgtataaca ctgttgaagc aatgaaagcc ggtgcttttg attatgtggc caagcctttt
     5401 aacattgatg aaattgaaga actggtaaaa cgtgcgcttc gagttccgct gagtactccg
     5461 gaaactaaaa gaataaaatc cggaaaaaaa gcagacgatt tactggccgg tcaaagcaaa
     5521 gtgatgcgcg acctgtataa ggcaatcggt cgagtcgcag ctacagattt gacagtactc
     5581 attcaaggcg aaagcggaac agggaaggaa ctgattgcac gttccattca ccagcattct
     5641 ttacgctcca gtcaaccatt tgttgcaatt aactgtgctg cgattccttc agagttgatg
     5701 gaatcagaat tattcggtca cgaaaaaggt gcttttactg gtgcaaccga acgcaagcgt
     5761 ggtaaactgg aacttgccag tcagggaacg ttgtttctgg acgagattgg agacatgccg
     5821 atgaggctcc agagtaaact cttgagggtc ttgcaggaaa aacaatttga acgagttggg
     5881 gggcacgagc taatcacgac aaatatgaga gtgattgctg ccacgcacca gaacctggaa
     5941 gatttgataa aaaattcaaa cttccggact gacctgtatt atcggctgaa tgtttttccg
     6001 cttaacattg ctccgttgcg tgaacgacgg gaagatattc cggttttaac tgaacatttt
     6061 ctgcagaagg gaagcaaaga aatggctgtc ggacgcaaag cagtagaacc cgaagtgatg
     6121 cgtgtgtttg ctctttataa ctggccggga aatatccgtg aattggaaaa tactgtcaag
     6181 agcttgatga taaccaatgt cactggcaca ataatgttgg actctttgcc aaaaaatttg
     6241 ttcaggcctc agcctgtaac agattctgga gaaagtcttg aagaggttgc atctttaaaa
     6301 ctggaaggac tggttcggga tgcagttgaa ttcgacagag attcactgtt ggcggaagta
     6361 ctgcctcagg tagaacggcc cctgctgaaa ttactccttg aacaaactaa ctggaaccag
     6421 caaaaatcag ccagaatttt gggaattaac agaaacactt tacgtaaaaa aatcgaaacg
     6481 ctgggtctga ggaatcaaag agatattgaa aaatgaaaac aggaacctcc gcaacaatgt
     6541 ttcaaagggt ttatgatgtt atcctggaac gaaaaaatta ctctccggat aattcatatg
     6601 tagcctcctt gatggacaaa ggtatagatt ccattttgaa aaaaattggt gaggaatctg
     6661 ccgaagtaat tctggctaca aaaaacgaga acaggaaaga acaaatacac gaaattaccg
     6721 atctctggtt tcacttgctc atcctgatgg gatatcaggg gattacaata gaagatattt
     6781 cacaagaact gaaaaaacgg ttcggtcaat ctggcttaga agaaaaagta cagcgttaat
     6841 catttaacaa tcaacacata gaaacagcaa tgccttccgt atcaaaatct agttcaattt
     6901 caacaaaaat tgctcagtca aaaatcaggg ccgtgtttca gcccttcaaa gttcaaggaa
     6961 ttcagttccg gaatcgtatc cttgcaccgc caacagtgga cgatcacgcg gatgtacatg
     7021 gcaaagtaac actggcaata atcaaacact accgttcttt agcacggcag ggtgcaggta
     7081 cgataattgt ggagtctgcc tatgtgacaa agcaggggcg aagtcacgtc aaccagctgg
     7141 gaatttcaga tgaagagcat cttgagggtt tggaaaaatt agcccagggt gtaaaaaaag
     7201 agggtgccac tatcggaatc cgtctcagtc acgcaggtgc acagacttct gaaagtgctt
     7261 gtggtgaaca gcctgtgggg ccttctgtgc tgaattttgg tcgggatttt gacatcagcc
     7321 gtgaattcga tcaaggtgat gttgaagaga ttgtactcaa ttttgtgcat gctgctgaac
     7381 gtgcagaaga agtcggtatg gattttgttg aaataaatgg aactgaacaa caattgctgg
     7441 atcaatgttg ttgtataaaa cttaacaatc gtgatgacga atatggtaca gcaaagattg
     7501 agaatcgtgt gcaactggca ctggatgtag tcaactctat caagaaacgt gagtcggtca
     7561 aaattccact tagttattac ttttccatct ttgataaaat tgatgatgga ttcaagccca
     7621 aagatcttaa aaaaatgttg acattactgg aaaatgcagg tgtagatatt ttccatccac
     7681 tcgctatcca tgcaatgaat aagtgttttg aaaacaaaga acctttgtgc cactggtcag
     7741 caaagtatac tgacaagcca atgataatta atggcaacat taaatcaccg caattgctcg
     7801 aagaagcggc aacactcggc tgcgcagatt ggtttgcgat ggatcaaagt atcttcgacc
     7861 gcttgcagtg gtatcaattt ctaaaaagaa aaattgataa ataatattct gagtaaacca
     7921 gaaaatgtca cgttcaatta tcaaaacatc aaaggcgcct gcacccgtag gcccttattc
     7981 tcaagccgta agctgcggta acatcctgta tgtttccgga caaatcccac ttgatcctga
     8041 gagtggagaa ctggttggga attcgtttgc agaacaatgt caccgggtac ttttaaatct
     8101 taaaataatt ctggaagcag gaggttcggg attggaacgt gtactgaaag tcacaattta
     8161 catgaaaaat ctggctcaat ttggtgagtt aaatgaaata tactcagaat attttgatgt
     8221 ttctaaacct gcacgggcat gtgtagaagt aagtaattta ccaaaaggag cagatgttga
     8281 aatggatgca attgcagaaa tttcttctgt taactaataa tattcaacaa taaaaaaatc
     8341 aaactgatgt cagcattaaa tatagcaatt gccgggttcg gtacaattgg cagtggtgtc
     8401 gcacacgttt tagaacaaca tagcaaagaa cctgatgcaa ccattaatct cgtgggaatt
     8461 ctggaaagtg atactaaggg gaaatttgcc aggccatggt tcgaaaaaag accggaattg
     8521 ttttatgata atccggataa acttcttgct gattcacagg ttaatgtaat tgttgagacc
     8581 ataggaggaa gcggattttc ccgtgaactg atcacaaagg ccttaaagaa cgggaaacat
     8641 gtcgttactg caaataagga tttgattgcg gttcacggtg ctgaactgat ggcagtcgcg
     8701 cgagaaaacg gacgtcattt gctttttgaa gcggctgtgg ctggagcaat tccggttttg
     8761 cggttactta aagattatct gcaagtacag gatattcagg gtatccgggc aattctgaac
     8821 ggtacaacaa actacattct aagtgaaatg gaatgcaatg acctttcttt tgagactgca
     8881 ctttcacagg ctcaggaatt gggatttgca gaacaggatc cgacaaacga tatcaaggga
     8941 attgatgccc gttacaaact cgtaattctg acttatttaa ttactggtca gtggtttgcg
     9001 cctgagcaaa tcaatttgga gggaatcgat cacttggaac tggctgattt tgaatatgct
     9061 gaacgaatgg gccggagcat taaattgatc gcaaatcttc gcctcagtga tcaaggagtt
     9121 caggtttatg ttttgccgct catgattgaa aaagacgata tgctggcaaa aataaccgga
     9181 tcaacaaatg ctgtgacgct ttcagggaag tatgcagatg acatcaccct gatcggcaaa
     9241 ggtgccggca gtctaccgac tgcctcggcg attgtttctg acctgaacaa aattcaaaga
     9301 caccctgaac cattccctcc tcctccttct aacaagaagt tagagattga atcgatggac
     9361 aacatacaat ttcgccacac attacgcttt gaagttcggg accagccggg aatattcggt
     9421 cactacggca gtatctttga gcggcattca attaacatat atgctttgga gcagttgccg
     9481 cagtaccacc gtattggtaa agaaggcgag gaaacagtga ttttcacact gacactccag
     9541 aactgtaaag aaggattggt ccacaaagct ctcaaagaaa ttaaccaggc agactacatg
     9601 ctgaaacccg tagtactcct aagggaaatt gtctgaagtt ttaagaatac tgttccacag
     9661 taatcaactg acaaaaagca ctcgttaata aaaatatgtt caagtttatc ggtacatacc
     9721 gtctttatat ttccatcgtc gctaccctca ttgttctgtt tttcctaatg tatcaaacat
     9781 ataacgagca aggtacatcc aggtttcaaa tggcagtaca aaccataaca taccccgtgc
     9841 agacctcagt gcagtctgtc gtatcaacta taaaaaatac ctggaattct tacataaatc
     9901 ttattgaggt taatgaagag aataagctgc tcaaacagca gttgctgaat atggaagaga
     9961 agctgaatca gcacattgag aattctgtac agtttttcag attgaggtct caactgctct
    10021 ttgcacaaaa aaaagcccac aaggaaatat tttcagaaat tattggagag tctgcagaca
    10081 acactcatca gattcgcttg ataaaccgcg gcagcaacca attgcttcag cggaactaca
    10141 ttgttatccg caaagagggg ttggtcggcc gtattcagtc tgtttcacct tatcagtctt
    10201 cagtacaact gattattgac caccgcagcc gtgttcctgc tttgattcaa cgtaatcgcg
    10261 ttagaggttt gatttatgga actcacgatg gcatggaaat gaggcaaatt aatcagcatg
    10321 cgaaaattaa aattggagat cgcgttattt ccagtggtct tgggaatctg tatcctaaag
    10381 gaatcctaat tggctgggtc agtggaatca accacgaacc ccatgaactt ttcaaaactg
    10441 caagattgga cagtgctgtt gactttaatc aaatagaaga ggtatttaca attttgcctt
    10501 ccaaatctga ttctaatttg tctgttgaat gatgcgatta ttcatgcttt ctttagggtt
    10561 tttgttaatc ggtgtcttga tgaacatcgt tttcaattac tattttctgc cagtaggttt
    10621 aaaaataaac tggatgctta tttttatact tgtgctgaca ttccgtcatt cttcaatttc
    10681 gctgtcattt ctcggtgtat ttgcgggaat gattttagac tcactttcgc atggttacat
    10741 agggctttat ggaatttctt tttttgtaac cctgttactt gcccgtcagt taattaaatt
    10801 attttacgca aatacttttt ttgcagtcag ccttgctgtc tcagttatga caatcctgga
    10861 gggttggata tctctatcca ttctgggcat gctggaaact gagctgaacc aatcttctct
    10921 aatgctgacc tcaactcttc cacttgccgt tttgcatggg ctggtaaccc catttattct
    10981 tcaaagtgta atttggggag aaaatcattt cataggggat acagcatgaa actgaatcct
    11041 cttgattttg aagagttgga ccggattcgg attcgcctca ttattttggg tggagtcgta
    11101 attttgattt tctccctgct ggcttcgcgt ctttggtatc tgcaaatttc ccagggcgaa
    11161 aaatatctcg aattttctca gggcaaccgc attcgtctgg ttccggaacc tgctttgcgt
    11221 ggaaatattt atgacaggaa tggagtttta ctggccgaaa accgccctgc ataccaactg
    11281 ctcctgataa gagaggacac gccaaatctg gaaacaactt tgcacaaagt ttcagaatct
    11341 ctcaaaatct cctattctgc actacaggaa aaaattgatg ccaatcagga tttggctcag
    11401 tttaggccaa ttatacttga agaagacttg gaatatgaaa aagccatgca tctggaaaca
    11461 tttcaggatg atttcccagg tgtttctgtt gtggtccgtc caagcagatt ttatccacaa
    11521 aaaaactttg catctcatct tatcggctat gtcggaattg tccaggaaga ttggtcagaa
    11581 ttgccggcag agaaacggag ttccagccag attgtcggtc actccggagt tgaacttctt
    11641 gagaacgatc acatgattgg actcgatggc ggccgacagg ctgaagttga tcatatgggg
    11701 agggaactgc aggttttagg agaacctgtc ccctctgtgc ctggaaaaaa tatcactttg
    11761 agcctggatg tccgtctcca gaaagttgca actgatgcca tgaagggtga aagcggtgcg
    11821 gtaattgtca tgaatccaaa aaccggcgga attcttgccc tggcaagttt tcctgatttt
    11881 gatccaaatt tgttttccgg aggaatagat ccggaaaatt ggaaacgtct cttggaaaat
    11941 ccgcaacatc ctatggaaaa caaagctatt caaggtctct atgctcccgg atcaattttt
    12001 aaagtagtca cagcttatgc cggacttgaa aagggtgcta ttacacctga aacagaatat
    12061 gtctgtaatg ggttttttca tgttaaaggc cgtagtgaac catttaaatg ctggaaggaa
    12121 acgggccatg gaagagttaa actgcatgac gcaattaaag gatcatgcaa cgtcttcttc
    12181 tataatactg gaatgggagc aggagttgat gagttgcata aattcgcaag cctgttccgc
    12241 ctgggaaaat taaccggagt tgggctcttg aatgaaaaag ccggattaat tccaagtagc
    12301 agttggaaaa aaagagtttt taacgaacca tggtatggcg gtgaaacccc ttccatgtca
    12361 atcgggcagg gatttattat tgttacgccg cttcaggtaa ttaatatgat taatattctt
    12421 gcaaatgacg gaatgtgggt gccacccagg ctttttttgg aagatgaagg aacggcacct
    12481 caacaaatac ctttaaacaa agaatatttg agcttaatcc gcaagggaat gattgcagtg
    12541 gtaaatgaag ctggtggaac tgcccgtaaa gttcagtttg aagaatttac tgttgctgga
    12601 aaaactgcga cctcacaggt tatcagtcac aaaaccatgg agacacttga cgaagaagca
    12661 aagctaaaaa aagaatttca gaaccatgcc tggtttgtag cttttgggcc agaggaagat
    12721 ccggaaattt cagtgatagc actggttgaa cacggtggtg gcggttcaag agttgctgct
    12781 ccagttgttc gaaaaatact tagctactac atcgacaata tttacaaacc aaaatctaaa
    12841 aaaacagtgc aaactaactt cgagtcaaaa aatttcaaat tcagtacacg tcttcaaatg
    12901 gccttcaact gaaatcctga ttccagacag gaatgtatgc aattacctgc atcgtctgca
    12961 actttataaa caatctgctt taaactctca taaaatgtcc cagtttatac agcatcgccc
    13021 tttgtgtctg gcctcttctt cacctcgtcg caagatgttt ttgaaaaaat atggcctcca
    13081 atttgactgt cacactccaa taattaatga aacaccggag aaaagcgagt ctgcaaaaag
    13141 ttttgtcaaa cgaatggccc atgaaaaagc cctgcaggta caaaggacat actcaaaact
    13201 ggcagaagat gtcataatcc tcgcaggaga tactattgtt tattttgacg gaaaaatact
    13261 ggggaaaccg caatcaattc aggatgccag gacaatgctg actcaattta gtggaaggac
    13321 acatcaggtt ttttcgggat attcaatctt agattcctct tccggaaatc atacaacaga
    13381 tcttgtttgt acggaagtcc gttttttaaa attaaatgcc gacctgcttg agtggtatct
    13441 taactccaaa gaatcttttg ataaagctgg tgcatattca attcaaggtt taggtgcaat
    13501 tttagttgaa tcaatctccg ggtcatacag caatgttgta ggatttccca ttgaatgcat
    13561 aattccaaac ttgaccgcaa atggctggat ttcctttgca gaaaatactc aacatgaggt
    13621 gtacggatga agtattattt attcattctc gttgcagtat tcctgattcc aacgccaaca
    13681 cacgccattg agttcgaaaa ccgcttgccg gaatcagtct gggaagtcga aatgcgtttg
    13741 cagcatacgc cggtttacga tcgggctttt aatggttatg gagaagaagc acctttgcag
    13801 caactcatgc tctgggacag agaatggcga gattcggtcg ttggtaagtt gcagcgtgaa
    13861 gagcagagac ttgaaatccg catggcatac ggtctgactg aaaagtggat gctggaggca
    13921 actatacctt tactgcaaaa aaaacaaacc tctactctca aaattgaatc cgctacagct
    13981 attcagcaga aagttctcga aaatttggct tcagaaaatc taagtggact tggtgatatc
    14041 aagcttatat ttgccaaaga catagttacg actacgacct ggcataatcg cggcggtttc
    14101 actttgcgac tgcctacagg tacaactggg actccccgcg gtatttctac aaattcaacc
    14161 ggagagggcc atggctcagt cggtgcctat tttcatttta attggtatcc tctgacacac
    14221 gggattcgga atgcattcag gatagaaggc acaaatgaac tggttggcaa acgtgaaaca
    14281 cttgatggag aaaaaggata ttactctgca gggcatcgcg tagacctttt ctacaactgg
    14341 agtattgaga ggcaaaatat atttgccggc acagaactgc attattacca acaggcagaa
    14401 agcagtttgc caacaggaaa atctaatgct gcttttctta aagagataaa ttttgaattc
    14461 ggttacggta acctgagtga acttgagcaa aaaccactgt ctaccccatg gcaagtccgc
    14521 ctgggttaca ctcgcccact tgccggacaa aacaccccta ttgcaaaccg ttgggaacta
    14581 agttccacct tctttttctg atcttttcca gcagaattat ttaaaacagc acagcacatt
    14641 ttttatctaa aggaacttcg tgaatttgga agatttgaac cattgtgata tcaaacaggc
    14701 acatattgaa ctgcttaaat gttgcggttc cagccaatgg actgaaaaaa tgttagcagc
    14761 tcggccttac aagtcagtat cgcatctgtt ggagctggca ggtcaaattt ggtctgattt
    14821 gggagaagtc gattatttgg aggcttttgc tgctcatcca aaaattggtg ccagtaaacc
    14881 tccggataat gcaaaaaata cggaaagctg gacgagcaaa gaacaagccg gaatgatgtc
    14941 cgcagatgag cagacaaaat tgaaattgaa aaccgaaaat caaaaatatg cagaaaaatt
    15001 cggttacata tttatcgtct gcgccacggg aaaaagcgca tcggaaatgc tggaactgct
    15061 ccggattcgg ctggagaact ccccggaaac agaactaaag attgcagctg gcgaacaaat
    15121 gaaaattaca aatttgcggc ttaataaaat gctgagagac gcataattta ttttgtgaga
    15181 aataccagtt tctgcgttac tgttaacttc cccgatatgt gctgtaacca aacggactca
    15241 acagcaacgg aatgtgataa tgctgctccg gatgattgag taaaaaacga acttcaattt
    15301 ccggataaaa ataagctgtt ccctgctgct caaaataggg acctgtctca aaaagcatcc
    15361 gataaactcc cgaattcatc atctcatctt cctgaatcaa attcgcaatc cgcccgtcag
    15421 aatttgtcga tccggacgct atttcctgcc aggttttgtc agtttgttgt tgttctaagc
    15481 gtatccgtat atttgctccc ggacagcctt gagaggtatc aagtacatgg gtagtgatag
    15541 gactcattag ttactcctat gaaattattt tgattgtata cagatttaaa ttcacttgat
    15601 ggatgcacct tcgtcaaccc atcttccaag aataaccctt tcttcgtgtg tcattccggt
    15661 tttatttcca agcggcatag caattgtcgt aactgactga attttaatga gggttgccag
    15721 tgcatgaact ctttcgttat tgtcaaacat aatgcctttg ggtgcaattc tgaaaacgtc
    15781 atcggtaggc cttgaagaat gacaactcac acaacgctga tccaaaatgg tgcgcaccag
    15841 ggcatacgga atatgctctg ttccaaatgt aaccgtgtcc ggtgaatttg aaatcgtggc
    15901 atccggtttc gtcacataaa taagcgcaaa aattgctgcc atggccaccg gcaaaatcca
    15961 aatattcaaa tgccctttat ttttgagatt gaaaaagtgt cgtacggtgg ctccaatcac
    16021 gattatcgct atcaaaatcg cccaattaaa ataatgcgca aaagtgtttg ggaaatggtt
    16081 gctgatcata atgaacacga cgggcaaggt catgtaatta ttatgtaaag agcgttgttt
    16141 tgcttttatt cccagagaag cttccggtgt ttccccagct ttgactgctt ccaccagttt
    16201 ggtttgtgag gggatgatac gccgccatac attgcccacc attaaggttc ccatgaccgc
    16261 cccgacatga atgtaagcgg cacgtgagcc aaaccattga tgaaaccacc agatgattcc
    16321 aaacaaaagc aaaaaggaaa tcatcactgg aaaccagcct ttctcaccca aagcagaagc
    16381 ccaaataaaa tcataaacta accaagacgc caaaatcgtt cccaagctga tactaaccgc
    16441 cattccataa cttaaatctg cgatttctga atcaatcaaa taaactccgg aacccgtgta
    16501 atacacaatt ccgagcagga aaataccgct gatccaagtc gaataagctt cccatttaaa
    16561 ccaatgcaga actcttggga tttcggaggg tgagatcatc gttttttcca attgataaaa
    16621 acccccactg tgtatcatcc acaactcgcc ctccacttta ggttcctgat tttccggcgg
    16681 agtgagctga ctgtccagcc aattgaaaaa aaatgaagcg ccaatccaag caattcctgt
    16741 aattacatga atccagcgga caaccagatt taaccattcg ataatatatg agtccataaa
    16801 ttttataaga gattagattt tatgttgtaa ctgacttgaa ataaaggtta gtattttttg
    16861 gcaaatatag ccaataattt ttggaaataa catggaaaat tatccccgca acatgcttgg
    16921 ctacggccaa aaacctccgc atcctgagtg gcccgggaag tcccggattg ccgtccaatt
    16981 tgtcatcaat tatgaggaag gaggagaaaa ctgtat
//
LOCUS       DTVD01000247           16937 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_633_length_16937_cov_262.679659, whole genome
            shotgun sequence.
ACCESSION   DTVD01000247 DTVD01000000
VERSION     DTVD01000247.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_16937_cov_262.679659"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="EYO68_09235"
     CDS             <1..1497
                     /locus_tag="EYO68_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HIC08020.1"
                     /translation="ERLFYNTPARLKFLKTTATELQHIQQHLVQKSLAYPHIHFRLTH
                     NRQLLLNLSGGQALETRIQQLYGEEFKEILMPVKHEETYLQFSGFISFPSKPRTSRRW
                     QYIFVNERNVKSPSINHGIYDGYGTFLGKGQHPVFFLNLRIDPTEIDVNVHPAKTEIR
                     FRNNQLVHTILTDQISRALKEGASRRFFGREHSHSKMSHTGVTGQIDLPLDDPLPLGN
                     ESAEMLFPVNKTRTKEARDLPPRGSLRPEKSHKLTAFPQENRIAEIRPIPKKTQDSAS
                     TTTLETPDSAPGNLPENEIRNLCLISPLPLVPKKIRVLGQFRKTYIIAENDAGLVLIE
                     QRALHERLIYDTYVESLQKKEVKIQTFKVSLLLELSPQESVLLEQSLVPLALSGFAVS
                     PFGGSTFAVDSIPECLSEDQVEKVVREILDRLALFGKRGQNEEILRDVCQVVSEHASM
                     PYEQELGQSEMDCLLAQWEELDSPMASIKEAPIMVEFSLQELEKRLRR"
     gene            1561..2526
                     /gene="trxB"
                     /locus_tag="EYO68_09240"
     CDS             1561..2526
                     /gene="trxB"
                     /locus_tag="EYO68_09240"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIC08021.1"
                     /translation="MILTTKTKMEKTHHKLIILGSGPAGLTAAIYAARANLNPVVVSG
                     REAGGQLMITTDVENYPGFPEGIQGPEMMDLLKAQAARFDTEFFSGDVVEVDLSQRPF
                     KLTLEIKDTLTCDSLIISSGASARWLGMDSEKEYSGRGVSACATCDGFFFRDQDVAVV
                     GGGDTALEEALYLANICKSVTMIHRRDEFRGSKIMQKRAFDHKKISILWDTVVEKVVG
                     NSKDGMTSLKVRNVKTDKVKEHPITGLFIAIGHTPNTNLFKDQLIMDKNGYLKVTPGT
                     TYTSIEGVFASGDVQDHVYRQAVTAAGTGCMAAIDAERWLAEQTG"
     gene            2611..3099
                     /locus_tag="EYO68_09245"
     CDS             2611..3099
                     /locus_tag="EYO68_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIC08022.1"
                     /translation="MNNKSLPAIVAVQTAVMLGLLYYLWNQETNEILNEKNFLFLAIF
                     SISCLLLAIVLHFKKPITYEEGTRRDGTVKWFNSNKGFGFIEQDTGDDLFVHQSEIRQ
                     AGFRFLNQGDRVEFEVGTGKKGPVALKVTRTQAADPEKYPNFNEEPDEALINKVSETQ
                     LN"
     gene            3103..4326
                     /locus_tag="EYO68_09250"
     CDS             3103..4326
                     /locus_tag="EYO68_09250"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIC08023.1"
                     /translation="MNQEKLVLAYSGGLDTSVLVHWLAQQGYEVIALCLDLGQKVEDL
                     DAILEKGKKAGAAKVLIENVEEEFVQDYVFPSIHWNALYEGTYLLGTSLARPLISRKQ
                     IEVAEREGAVAVAHGATGKGNDQVRFELSYYALNPNIRVVAPWKIPEFYKKYPGRTEL
                     LAYAEKYGIPVKASKEQPWSSDENLMHISFESGMLEDPWQAPLPEMFELSQSPKEAPV
                     ESQEILIEFLKGNPVAVDGKKFSPAKLLTYLNELGGRHGIGRIDMVESRFVGMKNRGV
                     YETPGGTILHIAHRAMESITLNRGVINLKDSLMPRFAQLTYDGFWFSPEMEILLEMVK
                     KTQEPVNGTVRLELYKGNCMVTGRKSPNSLYDEDMATMEADQGTYDPQDAIGFIKLHA
                     LPLRIQAGLKANSKT"
     gene            4369..5190
                     /locus_tag="EYO68_09255"
     CDS             4369..5190
                     /locus_tag="EYO68_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08024.1"
                     /translation="MKLQKLFPLLTVVFFLSTCILLYLLYFSVPQTLPISQSAQIEIT
                     VPEQTVISPEKPQISEEEIQRLLDIENEQKELVLLFERVSADLKEATGSKQILNQLSL
                     GRFILRDFEKRFLNNKLFRKAGGFLSFMREKTKSYLSSGKLAVNPAGIKKKDLLNFRR
                     ELTSWLLTRSNEEIEAVARFLSFFYAETLYDIPMTMLASRLTPELEEINFSTPVLLQK
                     VLTTASRDVNGEMNEKKPTDVAEYSMAQQFFFRRGPKISAKAVVFLKAYLSYTDL"
     gene            5293..6282
                     /locus_tag="EYO68_09260"
     CDS             5293..6282
                     /locus_tag="EYO68_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIC08025.1"
                     /translation="MEIRVLSSDDVQKALPMAEAIEGMKRAYSRLSSGRVEMPLRSRV
                     HVPEQEGVLLTMPAALPDDSEMAVKLVTVFGKNPARGFPLIHAAVLVLDTENGQIKAL
                     MDGEVLTAVRTGAGVGAATELLASTDAVSVAIIGSGKQACSQLDAVCTVRSIRQAWVY
                     SPNPEHAQNFVEEMSGVGPIPKDVQAVTSSAEAVKNADIVCTATTSSTPVISFKNLKQ
                     GAHLNAVGSFQPSMQEIDSDTIQNALVFVDSRESALIETGDIVTPLKQGLITADKIVA
                     EIGELINGTKSGRTSPEQLTFFKSCGVAVQDAVSAGIALKNAERENLGTIVSI"
     gene            6286..7443
                     /locus_tag="EYO68_09265"
     CDS             6286..7443
                     /locus_tag="EYO68_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC08026.1"
                     /translation="MSRLPKTASVVILGGGVMGASTVYHLASRGYKDVLLLEREKFFG
                     TGATGRCAGGIRYQFNTEVNIRLSQKSLPMIDAFEEETGQSVLVQKCGYLFALTREKD
                     VEVFRKTVELQRSLGVQTEWLTGDEVRSIAAPCDFPDALAGSFNAKDGLADPNSIVMG
                     YINAARRHGAVCITDCSAIGIDVKNNQIRKVQTSLGTIETKTVVNACGPWSASLGKEI
                     SLEISVFPLRRQWFVTEAIQELPEDFPFVIDFAQSLYFHREGSGLLTGMSNPNEKIGD
                     DQRIDSKWELKHIESAIQRMPLLGNSGIQARQAGLYEMTPDAHPIIGPTPVEGFYLLS
                     GFSGHGFMQGPICGKLMAEILIDGEATTVDISKLDYNRFAEKRLIPEYNVV"
     gene            7548..8927
                     /locus_tag="EYO68_09270"
     CDS             7548..8927
                     /locus_tag="EYO68_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIC08027.1"
                     /translation="MLNENTVAKILEYGLQQGADFTEVFVEDSVASSINLLDQKIEEI
                     NSSNSFGIGVRLLYGNEAYYGYSSDPDEANLLKLTGNLGQSQKEGQSGDMQSLQPQSI
                     EDIHHIAVNPETVSKSERVELLRELDNKTRNHGDDIQQVTVNMSEKKRSVLIANSEGL
                     WAEDSRNYSRMRLSAVAEKGDGPQTAAESPGVLGGYEFFQDLNLEELAENAAQSALRM
                     AAAGYIDGGKMPVILGNGFGGVIFHEACGHPLETEAIRKNASPFCEKIGKRVGQSILT
                     AIDDGTIANKWGSCNVDDEGTPTQKTVLIENGILNNYLSDRIGAGQVGISRTGSARRE
                     SYKFAPVSRMRNTFIAPGPHEIEEMLRSIKFGLYAKKMGGGSVNPGTGEFNFAVQEGY
                     AIRNGKIAEPVRGATLIGKGFDVLSQISMIADDLDLAAGMCGAASGWVPTTVGQPTLK
                     IDSILVGGR"
     gene            8984..10279
                     /locus_tag="EYO68_09275"
     CDS             8984..10279
                     /locus_tag="EYO68_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIC08028.1"
                     /translation="MRFFEQSVETMPREALELLQIEKLYSMLGKIYGSNRFYTGKLEA
                     AGIHPEAIQTLSDLASLPLTTKAELVQAQEDALPFGTNATFPESAYSRFHQTSGTTAT
                     PLRVIDTPESWEWWGRCWGYVLAGAGLTENDRLFVPFSFGPFIGFWAAVEGAKGIKAL
                     MIPGGGRDSLQRLHLMKELGATAMCCTPTYALRLAEVARDSGFDLCEIPLKTLIHAGE
                     PGANIPATKARIESVWNTKCYDHAGASEVGAHSFECEIQPNGTHAIESEFIVEVLNPE
                     TLEPVPAGEQGELVITNLGRIGYPVIRYRSGDLVRLNPESCECGRTFVRFEGGVLGRS
                     DDMVVVRGINVFPSAVENLVRQCEAVEEFRITVSTEREMAHLTIDLEISKNADEETSR
                     HTVDQAIQNELSLRPEIKVVPSGTLPRFEMKAKRFHVEK"
     gene            10371..10856
                     /locus_tag="EYO68_09280"
     CDS             10371..10856
                     /locus_tag="EYO68_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC08029.1"
                     /translation="MKQLLTLKINGETHEVWTSTHKTLLEVLREDLYLTGTKHGCELG
                     ECGACSVLIDGEPVLACLILPVEVQGQEIQTIEGLAKNGVPHPLQTAFAEQGASQCGY
                     CSPAMLMTAKALLEINSNPDSNTIRKALSGTLCRCTGYSKIIEAVEQAAGELAVMRQN
                     S"
     gene            10889..11269
                     /locus_tag="EYO68_09285"
     CDS             10889..11269
                     /locus_tag="EYO68_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08030.1"
                     /translation="MRFFRIITGIGITLLVSSCSVSTFGKFQADEGIPSFIQVGKTTR
                     EEIMTTLGEPLVHRFVVGKETLIYNHERGEYFFLYGTYEGNELVIRLTDQIVSEVKLE
                     KTGSGWGFFAPATSNNPGTRRSAR"
     gene            11274..11666
                     /locus_tag="EYO68_09290"
     CDS             11274..11666
                     /locus_tag="EYO68_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08031.1"
                     /translation="MTKFYILLPITGILLFMLFVGCAKTRIGKVELSDEKLPNFLKVG
                     TTSAQEVLEQIGEPFGYREQENRSAMIFLDFQEDYVNLLITEIRLEKAYRLDLVFQDD
                     ILQKAEIKKEGWGFGANMDPQLLQLLAR"
     gene            11705..14140
                     /locus_tag="EYO68_09295"
     CDS             11705..14140
                     /locus_tag="EYO68_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidase"
                     /protein_id="HIC08032.1"
                     /translation="MSENKDFQAGLTWIGKSWAQVDGLAKAKGETVYADDMFLPRMLF
                     AKMLRSPHPHARIKSIDLTQALKRPGVVAAMTGKDLPIPFGILPVSQDEESLCVDKVR
                     MIGDPVAAVAAIDEETAESALEDIVVEYELLPSVLTIEDGLKSLEETEISKTQIHEYA
                     DSGNIHKQVALEFGDIETGFAEADHIREELYYYEGNTHLPIEQHSAVAQHGADGRITL
                     WSSTQTPHYVHRALAKVLEMPASQIRVIATPVGGGFGGKTDPFQHEMVVCKLAMMTGR
                     PVKLTLTREEVFYTHRGRHPVLMWVKAGMKSDGSITALHFRNFLDGGAYGSYGVASTY
                     YTGALQTVTYPIANYKFEAMRVFTNKAPCGPKRGHGTPQPRFALEVLLDKFAEDLELD
                     PAELRLRHLIPDNSKTVNHLTVTTNGLGECIRKVTEASRFQQQRTGSSLGKGFGLGCG
                     SYLSGAGLPVYWNKMPHSGVQIKIDRGGGVTVFCGSTDIGQGSNSVLAGIVAEELGID
                     IGNIIVVTADTDLTPVDLGSYSSRVTLMTGNAAIDAARPLREKILSAASEVLKIPAEK
                     LMLRNSSVLSKNGSGEPVSFVDAVQWAETKYGMLGSVGSYTPPSHGGKYKGAGVGPSP
                     NYSYSACVAEVTCDPESGIYRVDKLWMAHDIGQAINEKLVIGQVEGSAYMGLSEAMME
                     EQIYRTGKDNPGGLHKTPSMLDYKSLTALDMPEIETFLVETHDPEGPYGAKEAGQGPL
                     LPIMPAIANAIYNAVGVRVDEVPITPEKIYQGLKELKSGKPGGSGVIGRVGPKKFPKI
                     TFPEAIKVKSVDS"
     gene            14210..15226
                     /locus_tag="EYO68_09300"
     CDS             14210..15226
                     /locus_tag="EYO68_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase"
                     /protein_id="HIC08033.1"
                     /translation="MLRLPPFEYQAPNSIAEAVALKAECGDSSMFVSGGTDLFPNMKR
                     RQFTPKILIGLRKLAELRSISLDDSGNGLMIGSGVSLSEVAEHPEILNNFPALAKAAS
                     LVSAPQLRNMGTIGGNLCLDTRCNYYNQTFPWRKALGFCLKKPESPMQNDAICWVARS
                     SPKCLAVSSSDCAPVMVALDAEFHLMNPDGKRIVPAGEFYKNDGMDFLNKTPDELLVS
                     IHLPLHEGWKMNYRKLSRRGAIDFPVLGVAVALRLDTSGNCMEARIVLGGVASCPLRA
                     FEAEKLLVGGSLTAEGSLTRIENTAQVAAKLAKPMDNTDITLGFRKKMVMQFVAKTIK
                     EVLK"
     gene            complement(15233..15886)
                     /locus_tag="EYO68_09305"
     CDS             complement(15233..15886)
                     /locus_tag="EYO68_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC08034.1"
                     /translation="MQFQAICFDLDGTLLDSLADLAHCTNTILSKRGFPEHPVDAFRY
                     FVGDGAKMLITRALPEEVRKEPLIEKCRQDFETAYRECWDEHTQPYDDIPVLLDELES
                     RKLKLTVLSNKPHEFTLLAVKHLLPNWEFELVLGQREGIPRKPDPAGMIDICEQLKIP
                     AKSFLYLGDTATDMKTATSAGCFPVGVLWGFRPEKELRENGAQAIINAPLELLDLLD"
     gene            complement(15900..16181)
                     /locus_tag="EYO68_09310"
     CDS             complement(15900..16181)
                     /locus_tag="EYO68_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIC08035.1"
                     /translation="MVKVDIVRAIQLGTDLQFGEAEKVVNDMLEMIKEKLEKGEDVLI
                     TGLGKFELRNKKSRPGRDPKSKRDYEISARKVVTFHPSKIWRDELNNNN"
     gene            16551..>16937
                     /locus_tag="EYO68_09315"
     CDS             16551..>16937
                     /locus_tag="EYO68_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2393 domain-containing protein"
                     /protein_id="HIC08036.1"
                     /translation="MFFRQHKRLFFLVIIVIVLSILTLYFQQIKLEELRTELAKVELQ
                     NVRVGAGTSKGKLGTAIFGVIQNNGEHTIKIATMNVNFIGEDGKSSKVHKFFPVNNYS
                     FSDSLPLEPGQSKEFGFPIDEIIPEDW"
BASE COUNT         4888 a         3440 c         4022 g         4587 t
ORIGIN      
        1 gaacgtcttt tttacaatac acctgcccgc ttgaaatttc tcaaaaccac tgccacagaa
       61 cttcagcaca ttcagcagca tctggtacaa aagtccctgg cttatccgca cattcatttc
      121 cgtctaacac ataatcggca acttctgcta aatctttccg gaggacaagc tctggaaact
      181 cgtatccagc agctttatgg tgaagagttt aaggaaattt tgatgccggt taaacatgag
      241 gaaacatatc tacagttttc cggtttcatt tcatttcctt caaaaccacg gacgagcagg
      301 cgctggcaat atatttttgt gaatgagcgg aatgtaaaat ctccgtcaat caatcacggg
      361 atttatgatg gttatggaac ttttttaggc aaaggacagc atccggtttt tttcctaaat
      421 ttgagaatag acccgactga aattgatgtg aatgtacatc cggcaaagac agaaatacgt
      481 ttccggaaca accagcttgt ccatacaatt ttaacagatc aaatttcccg tgccctgaaa
      541 gaaggagcca gtcgccggtt ttttggccgt gaacattcac attcgaaaat gtcacacaca
      601 ggtgttacag gacaaataga tctgccactg gatgatccgt tgcctttggg aaatgagtct
      661 gcagaaatgt tatttcctgt aaacaaaacc cgtacaaaag aagcaagaga tctcccgccg
      721 cgaggctcgt tgaggccgga aaaatcacat aaactcactg cattcccaca agaaaatcgt
      781 attgctgaaa tccgaccaat accaaaaaaa acgcaggatt ccgcttcaac aacaactttg
      841 gaaactccgg actccgctcc ggggaatctt ccggaaaacg agatacgtaa tctctgtcta
      901 atttcacctc tgccgttggt tccaaaaaaa ataagggtgc ttggtcagtt ccggaagaca
      961 tacattattg cggaaaatga tgcggggttg gtgctgattg aacagcgagc gctccacgaa
     1021 cgtttgattt atgatactta tgtggagtca ctgcaaaaaa aagaggtgaa gattcagact
     1081 tttaaagttt cacttttatt ggaactgtct cctcaggaat cagtgttgct ggaacaatcc
     1141 ttagtaccat tagccctttc cggttttgca gtcagtccat ttggagggag tacttttgca
     1201 gtagattcga ttccggaatg cttgagtgaa gatcaagtag aaaaagtggt cagggaaatc
     1261 cttgatcgac tggcattgtt tggcaagaga ggtcaaaacg aagaaatatt acgggacgtc
     1321 tgtcaagtgg tttcagagca tgcatctatg ccatatgagc aggaacttgg gcagtctgaa
     1381 atggattgtt tgctggcaca gtgggaagaa ttggacagtc cgatggcatc catcaaggaa
     1441 gcccccatta tggtggaatt ttctctacaa gaactggaaa aaaggttgag gcgttgatcg
     1501 gggttatttg cttatttgga attgacttga aactgccgga tgttcaaatg gtaaaataac
     1561 atgatattaa caaccaaaac taaaatggaa aaaacccacc acaaactaat aattttaggc
     1621 tccggtcctg cgggattgac tgcggcaatt tatgcggcaa gagccaactt aaatcctgtt
     1681 gtcgtctctg gacgagaggc aggaggacaa ttgatgatca ctacagacgt tgaaaattat
     1741 cctggttttc cagaaggaat tcaaggtccg gaaatgatgg atctcctgaa agcccaggcc
     1801 gctcgttttg acacagaatt tttttccggt gatgttgtag aagttgattt gtcacaacga
     1861 ccatttaaac ttacgttgga aatcaaagat acgttgacct gcgattcact gattatttct
     1921 tctggagcct ccgcgagatg gcttggaatg gattcagaaa aagaatacag cggcaggggg
     1981 gtttctgcct gtgcaacctg cgacgggttt ttctttcgtg accaggatgt ggccgttgta
     2041 ggcggaggtg atactgcgct ggaagaagct ctgtatttgg caaatatctg taaaagtgtg
     2101 acaatgattc atcgacgcga tgaattccga ggcagcaaaa ttatgcagaa acgtgctttt
     2161 gatcacaaga aaatctcaat attatgggat actgttgttg aaaaagtagt gggtaactcc
     2221 aaggacggaa tgacgtccct gaaagtgagg aacgttaaaa cggataaagt taaagaacat
     2281 cctatcacag gattgttcat tgcgattgga cacactccaa atacaaattt attcaaagat
     2341 cagttgatta tggacaaaaa cggttatctg aaagtcacgc ctggcacgac atatacgtct
     2401 attgaaggcg tttttgcttc aggagatgtt caggaccatg tttatcggca agccgttacg
     2461 gctgcaggaa ccggatgtat ggcggccatt gacgcagagc gctggttggc agaacaaacc
     2521 ggctgaacat aattgccact gcagaaaact aaattaaaaa attacctgca gtggatattt
     2581 aaaaaaaaca aatgaaaata ttaaaacaaa atgaacaaca aatctttgcc tgcaattgtt
     2641 gcagttcaaa ctgctgtgat gctgggactg ctgtattact tatggaatca ggaaacaaac
     2701 gaaatattaa atgaaaaaaa ctttcttttt ttagcaattt tttctatctc atgcctatta
     2761 ctggcgatag ttctacattt caaaaagcca atcacctatg aagaaggtac tcgccgagat
     2821 gggacagtta aatggtttaa ttcaaataaa ggttttggct tcatagaaca ggacactgga
     2881 gacgaccttt ttgtccatca gtctgaaatt cggcaggccg gttttcgttt cctcaaccag
     2941 ggggatcgtg ttgagtttga agtcggaaca ggaaaaaaag gaccggttgc tctaaaagta
     3001 actcgtactc aggcggcaga tccagagaaa tatcccaatt ttaacgaaga gcctgacgaa
     3061 gctctgatta ataaagtttc tgaaacacaa ttaaactgaa aaatgaacca ggaaaaactc
     3121 gtattagctt attccggcgg tctggatact tcagttcttg tccattggct tgctcaacaa
     3181 ggatatgaag taattgcact atgtcttgat ttaggacaga aagttgaaga tttggatgcc
     3241 atccttgaaa aaggtaaaaa agctggtgca gcaaaagttc tgatcgaaaa tgttgaagaa
     3301 gaatttgtac aggattacgt tttcccttcc attcattgga atgcgcttta tgaaggaact
     3361 tatttgctgg gaacttcatt agcaaggcct ttgatttcca ggaaacagat tgaagttgca
     3421 gagcgggaag gtgctgttgc cgtagcacat ggcgcaaccg gaaaaggtaa tgatcaggtc
     3481 cgttttgagt taagctatta tgcgctcaat cctaatattc gtgtagtagc accatggaaa
     3541 attcctgagt tttacaaaaa atatcccggt cgtacagaat tattagccta tgcggaaaaa
     3601 tacggtattc cggtcaaggc ttccaaagaa caaccctgga gttcagatga aaatcttatg
     3661 cacatcagtt ttgaatctgg tatgttggag gacccctggc aagctccact accggaaatg
     3721 ttcgaattgt cacagtcgcc gaaagaggct ccagttgagt ctcaggagat tctgattgaa
     3781 tttctaaaag gaaatccggt cgcggttgat gggaaaaaat tttctccggc aaaattgttg
     3841 acatatctaa atgaattggg cgggagacat ggaattggcc ggattgatat ggtagagtcc
     3901 cgttttgtcg gcatgaaaaa caggggggtg tatgaaacac caggtggtac tattttacac
     3961 attgcacatc gggcaatgga gtcaattacc ttgaaccgtg gtgtaattaa tctcaaggat
     4021 tcactaatgc cgcgctttgc gcaactgacc tacgatggct tttggttttc acctgaaatg
     4081 gaaattttgc tggaaatggt gaaaaaaact caggaaccgg ttaatggtac agtccgtctg
     4141 gaactttaca aaggcaactg catggtgact ggccgtaaat caccaaacag cctctatgat
     4201 gaggatatgg caacaatgga agcggaccag ggtacttatg atccacagga tgccattggt
     4261 tttatcaaac ttcatgccct gccgttacgt attcaagcag gtttgaaagc taattctaaa
     4321 acttagagcc ggactttact caaactattt tccaacctgg taagaagaat gaagctgcaa
     4381 aaattatttc ctctactcac tgtggtattt tttctttcca cctgcatttt gctgtatctc
     4441 ctttattttt ctgttccaca aactttacca atctcacagt cggcccagat cgaaattact
     4501 gttccagaac aaacagtgat ttctcctgaa aaacctcaaa tttcagagga ggaaattcaa
     4561 cgtttactgg atattgaaaa cgaacaaaaa gaacttgtat tactctttga acgtgtttct
     4621 gctgatctga aagaagccac tggttccaaa caaattctga atcaactttc cttgggacgt
     4681 tttattttgc gggatttcga aaaacgtttt ttgaacaata agcttttccg caaagctgga
     4741 gggtttttaa gtttcatgag ggaaaaaaca aaatcttatt tgagctcagg aaaacttgct
     4801 gtaaatcctg ctggaatcaa aaagaaagat ttgcttaact tccgcaggga actcaccagt
     4861 tggctcttga cgcgcagcaa cgaagaaatt gaggctgtgg cgaggttttt gagttttttc
     4921 tatgcagaaa cactttatga tattccaatg acgatgttgg cttccaggtt gactcctgaa
     4981 ctggaggaaa ttaattttag tacaccggtg cttctgcaaa aagttctaac tactgcaagc
     5041 agagatgtca acggtgaaat gaatgaaaaa aagcctactg atgttgcaga atattcgatg
     5101 gcacagcagt tcttttttcg caggggtccg aaaatatccg caaaggcagt tgtctttttg
     5161 aaggcatacc tcagttacac cgatctgtaa tccatttgaa tgagtctgtt gagaacttgg
     5221 ttctcttgtc taaatccaac gccggaatag tgtcatcttc agtttcactc taattatcaa
     5281 tggcccaaca aaatggaaat tcgggttctc agtagtgatg atgttcaaaa ggcccttccg
     5341 atggcagaag caatcgaggg catgaagcgt gcgtatagcc ggctttcgtc cggacgggtt
     5401 gagatgcctc tccgcagccg tgtccatgtt cctgagcagg agggagtgct cctgacaatg
     5461 cctgccgcgc ttccggacga cagtgaaatg gctgtaaaac tggtaactgt ttttggcaaa
     5521 aatccagccc gtggatttcc cctgattcat gctgccgtgc ttgtgttgga caccgaaaac
     5581 ggtcaaatca aagccctgat ggatggagaa gttttgactg cagttcgtac cggagctgga
     5641 gtcggagccg ctacagaatt gcttgcgtct acggatgcgg tttctgtcgc aataatcggc
     5701 agcggaaaac aggcctgttc acaactggat gctgtttgta ctgttcgcag tattcggcaa
     5761 gcgtgggttt atagtccaaa ccctgaacat gcacaaaatt ttgtggaaga aatgtccgga
     5821 gttggtccaa ttccaaagga tgttcaagca gttacttctt ctgcagaagc cgtaaaaaat
     5881 gcagacattg tctgtactgc caccacttcc tctactcctg ttatttcctt taagaacctc
     5941 aaacaggggg ctcatttaaa tgcagttggt tcttttcagc catcaatgca ggaaatcgat
     6001 tcagatacta ttcaaaatgc tcttgtcttc gttgattccc gtgaatctgc actgatcgaa
     6061 actggagata tagtcactcc gcttaaacaa ggtttgatca cagcagacaa aattgttgca
     6121 gaaataggag aactcatcaa tggaacaaaa tccggacgaa cctcacctga acaactgaca
     6181 ttttttaaat cctgtggagt agccgtacag gatgccgtct ctgccgggat tgctttgaaa
     6241 aacgcagagc gtgaaaatct cggaacaatt gtctctatat aaaaaatgtc cagactgcca
     6301 aaaactgcct cggtagtaat tctcggaggt ggagtcatgg gtgcaagcac tgtttaccat
     6361 ttggcaagcc gcggctacaa agacgttttg cttctggaac gcgaaaagtt tttcggtacc
     6421 ggcgcaaccg gacgttgtgc cggaggaatt cgttatcagt tcaatactga agtcaacatc
     6481 cggctttccc aaaaaagtct tcccatgatt gatgcttttg aggaagaaac cggacaatcg
     6541 gtgttggtgc aaaaatgtgg atatttgttt gcactgactc gggaaaaaga tgtagaggtg
     6601 ttccggaaaa cggttgaact tcaacgttcc ctcggcgttc aaaccgaatg gcttaccgga
     6661 gatgaagtac gcagtattgc cgctccctgc gacttcccgg atgcacttgc tggaagcttt
     6721 aacgcgaaag acggactggc agaccccaac agtatcgtga tgggttacat caatgctgcg
     6781 cgtcggcatg gagcagtctg cattactgat tgttctgcaa tcggtattga tgtaaaaaat
     6841 aatcaaattc ggaaagttca aacatctctt ggaaccattg aaactaaaac tgtagtcaat
     6901 gcctgtggac catggagcgc aagccttgga aaagagatta gtctcgaaat ttcagtattt
     6961 cctctgcggc gtcagtggtt tgtcactgaa gcaattcagg aacttccgga agacttcccg
     7021 tttgtcattg attttgcgca aagtctctat tttcaccggg aaggttccgg actgcttacg
     7081 ggcatgtcga atcccaacga aaaaattgga gatgatcaaa gaattgattc caagtgggaa
     7141 ttgaaacaca tcgaatctgc aatacagcga atgcccttgc taggaaactc cggaatccag
     7201 gcacgacaag ctggactgta cgaaatgact ccggatgcac accccatcat cggccctaca
     7261 ccggtggaag gattttactt gcttagcgga ttttcaggac acggattcat gcagggccca
     7321 atttgtggaa aattgatggc cgaaattctc atagacggtg aagccaccac cgttgacatc
     7381 agcaagttgg attacaaccg atttgctgag aagcgcttga ttccggaata caacgtagtt
     7441 tgattaacgt ttccggattg caccactgtc aattatgctt taaaatgatt gatcaaaact
     7501 gttatattat ttaaagttac tcgaaacaac ctttcccggg aaaaactatg ctcaacgaaa
     7561 acacggttgc aaaaattttg gagtatggac ttcagcaggg ggcagacttt actgaagttt
     7621 ttgtagagga ctctgttgct tcttctatca atctgcttga tcagaaaatt gaagaaatca
     7681 attcctcaaa ctcatttgga ataggggtgc gtcttcttta cggcaacgaa gcctattacg
     7741 gttacagcag tgatcctgac gaagccaatt tgctgaaact caccggcaat ttagggcaat
     7801 cccagaaaga agggcagtcc ggagatatgc aatcccttca gcctcagtcc atcgaagaca
     7861 ttcaccatat agccgtaaat ccagaaacgg tgagcaagtc tgagcgcgtt gaactgctaa
     7921 gggaactgga taacaaaacc cgtaatcacg gtgacgatat tcaacaggtc acggtcaaca
     7981 tgtctgaaaa aaaacgctcg gtacttatag ccaacagcga gggtttatgg gccgaggact
     8041 cacgaaatta ttccaggatg cgtctttctg cagttgcaga aaaaggcgat ggaccccaaa
     8101 ctgcagctga aagtccagga gttttgggtg gttatgaatt ttttcaggat ttgaatttgg
     8161 aggaacttgc agaaaatgcg gcacaatctg cgttgcgtat ggccgctgca ggatacatcg
     8221 atggaggaaa aatgcctgtg attttgggta atggattcgg cggtgttatt ttccacgagg
     8281 cttgcggtca cccattggaa acagaagcga tccgaaaaaa cgcatctcct ttttgcgaaa
     8341 aaataggaaa gcgtgtcggc cagtcgattc ttactgcaat agacgatgga actattgcaa
     8401 acaaatgggg ttcgtgtaat gtggacgacg aaggtacgcc gacgcaaaaa accgtactaa
     8461 tcgaaaacgg aatactcaat aattacctca gtgaccgtat cggtgcaggt caagtcggta
     8521 tttcccgtac cggttctgca cgacgcgaat cgtacaaatt tgctccggtt tcccgtatgc
     8581 gcaacacctt tattgctccc ggaccgcacg agattgaaga aatgctgcgt tccattaaat
     8641 ttggcctgta tgcaaaaaaa atgggtggcg gatctgtcaa tccggggact ggtgaattca
     8701 attttgcagt gcaggaaggt tatgctatcc gcaatggcaa aatcgctgaa ccagtgcgcg
     8761 gagcaacgct gatcggcaag gggttcgatg tcctgtcgca aatttcgatg attgccgatg
     8821 atctggattt ggcggcagga atgtgcggag cggccagcgg ctgggttccg acaactgttg
     8881 gtcagcccac tttgaaaatt gactcaattc ttgtcggcgg acgataattt tttttaaaat
     8941 tgtttctact tccgtagagc aacaaacaga taagaaacac ttaatgcgat ttttcgagca
     9001 gtcagtagaa accatgcccc gcgaagcatt ggaattacta caaatcgaaa agttgtattc
     9061 catgctggga aaaatttatg ggagcaaccg attttatacg ggaaagctgg aagccgcggg
     9121 aattcatccg gaagcgatac aaactttaag tgatttggcg agtttaccgc tgactaccaa
     9181 agcggagtta gtacaagccc aggaggatgc tttgcctttt ggaaccaatg cgactttccc
     9241 ggaatccgct tactctcgtt ttcaccagac atccggaacc accgcaactc ccctgcgagt
     9301 tatagacacc ccggaaagtt gggaatggtg ggggcgttgc tggggttatg ttttggcagg
     9361 agcaggtttg acggaaaatg atcgtttgtt tgtaccattt tcttttggac cattcattgg
     9421 tttttgggca gcagtcgagg gtgcaaaagg aattaaagcc ttgatgattc ctggcggagg
     9481 gcgggattca ttgcagcggt tgcatttgat gaaagaactt ggtgcgacgg ccatgtgctg
     9541 tactccaact tatgctttgc gtttggcgga agtggctcgg gattccggat ttgatttgtg
     9601 tgaaattcca ttgaaaactc tgattcatgc cggagaacct ggagccaata ttccggcaac
     9661 taaagcccgt attgaatctg tttggaacac aaaatgttac gatcacgcgg gggcttcaga
     9721 agtaggggca cacagttttg aatgcgaaat tcaaccaaac ggaacacatg ccatcgaaag
     9781 tgaattcata gttgaggttt tgaatccgga aactctggaa ccggttccgg caggagaaca
     9841 aggcgaactg gttatcacca atttgggtcg catcggttat ccggtaattc gttaccgttc
     9901 aggagatctt gtacggctta atccggagtc ttgcgaatgt gggcgcactt ttgttcgttt
     9961 cgaaggcggg gttctgggac ggtcagatga catggtggtt gtgcgtggaa tcaatgtttt
    10021 tcccagcgca gtggaaaatt tagttcgtca atgtgaggca gttgaagaat tccggatcac
    10081 ggtttcaact gaacgggaaa tggcacattt gacgattgat ttggaaataa gcaaaaacgc
    10141 tgacgaagaa acttctcgac atactgtgga ccaagcaata cagaacgaat tgagtttacg
    10201 cccggaaata aaagttgtgc cttccggaac tctgccccgt tttgaaatga aggccaaacg
    10261 ctttcatgtg gaaaaataag ttagccacag acaacacgga taaaaacgga tagaaaaata
    10321 tccgtgacta tctgtgcaaa tctgtggctg ataacaaaaa gaaaattaat atgaaacaac
    10381 tcttaactct caaaatcaac ggcgaaactc acgaggtttg gacttcgact cacaaaactt
    10441 tgctggaagt tttgcgtgaa gatttgtacc tcaccggaac caaacacggt tgtgagttgg
    10501 gtgagtgcgg agcctgttcg gtgttgattg acggagaacc ggttttggcc tgtcttattt
    10561 tgcctgttga ggtgcaggga caggagatcc aaaccattga aggtttagca aaaaatgggg
    10621 ttccgcatcc actgcaaacc gcttttgcag aacaaggcgc atcgcaatgc ggttactgtt
    10681 ctccggcaat gttgatgacc gccaaagccc tgcttgaaat aaattcaaat ccggattcaa
    10741 atactattcg aaaagccctg tccggaactt tgtgtcgctg tacagggtac tcaaaaatca
    10801 tagaagcagt agagcaagct gctggagaat tagcagtgat gcggcaaaat tcctaaatgt
    10861 tcgaagtaga gtcactatga agattaaaat gcgttttttc agaatcataa caggtattgg
    10921 gatcacactt ttagtgtctt cctgttcggt tagcactttc ggaaaatttc aggcagatga
    10981 aggaattccg tctttcatcc aagttggaaa gacaacgaga gaagaaatca tgaccacttt
    11041 gggagaacct ctggttcatc gcttcgtggt cggcaaagaa acgctcattt acaatcatga
    11101 acggggagaa tacttttttc tctatggaac ttatgaagga aatgaattgg tgattcgtct
    11161 tacagatcaa atcgtttctg aagtgaaact tgaaaaaact ggcagtggat ggggattttt
    11221 tgctccggca acttccaaca atcccggtac aagaagaagc gcaaggtaat cgtatgacga
    11281 aattttacat tttattgccg atcaccggca tcttactttt catgttgttc gttggatgtg
    11341 ccaaaactcg aattggaaaa gtggaattat ccgatgaaaa actgccaaat tttctcaaag
    11401 taggaacaac gagcgcacaa gaagttttgg aacagattgg agaacctttc ggctaccgag
    11461 aacaagagaa tcgttcggca atgatcttcc tcgattttca ggaagattat gtcaatttat
    11521 tgatcacaga gattcggctg gaaaaggcat atcggctgga tttggttttt caagatgata
    11581 ttttgcaaaa agcagaaatc aaaaaagaag gatggggatt tggtgcaaat atggatcctc
    11641 aacttttaca attgctggcc agataaaaca ctgtttttcc attttttcaa gcatcaaata
    11701 gtttatgagt gaaaacaaag atttccaagc gggccttaca tggattggta aatcttgggc
    11761 tcaagtagac ggtttggcca aagccaaagg tgagaccgtt tatgctgatg acatgttttt
    11821 accgcgaatg ctctttgcca aaatgttgcg cagtcctcac ccgcacgctc ggatcaaaag
    11881 cattgatttg acacaggctt tgaaacgacc cggagtcgtg gcggccatga cgggcaaaga
    11941 tttgccgatt ccttttggga ttttgccggt gagtcaggac gaggaatcgt tgtgtgtgga
    12001 caaagtgcgc atgattggcg atccggtggc ggcagtggcg gccattgacg aagaaacagc
    12061 agagtctgca ctcgaagata tagtcgtcga atatgagctt ttgccgtctg ttttgaccat
    12121 tgaagacggt ttaaaatcct tggaagaaac tgaaatttca aaaacgcaaa ttcacgaata
    12181 tgccgattcc ggaaatattc acaaacaagt ggctctcgaa tttggcgaca tcgaaaccgg
    12241 atttgcagaa gcagaccata ttcgcgaaga actttattat tacgaaggca acacgcattt
    12301 gccgattgaa caacactctg ccgtcgctca acacggagcc gatggacgga tcacgctttg
    12361 gtcttccacg caaactccgc actatgttca ccgtgctttg gccaaagttt tggaaatgcc
    12421 ggccagtcaa attcgtgtaa ttgcgactcc ggttggtgga ggatttggcg gaaaaacgga
    12481 tccgtttcaa catgaaatgg ttgtgtgcaa actggccatg atgacaggtc gtccggtaaa
    12541 attgactttg acccgcgaag aagtttttta cacacatcgt ggacgtcatc cggtattgat
    12601 gtgggtcaaa gccggaatga agtcagacgg ttcaatcaca gctttgcatt ttcgtaattt
    12661 tctggatggc ggagcatacg gaagttacgg tgttgccagt acctattaca ccggtgcttt
    12721 gcagacagtg acgtatccga ttgcaaatta caaatttgaa gcgatgcggg ttttcaccaa
    12781 taaagctcca tgcggtccca aacgaggaca cggcacgccg cagcctcgtt ttgcgcttga
    12841 agttttgctt gacaaatttg ctgaggattt ggaactggat ccggcagaat tacgcttgcg
    12901 acatttgata ccggacaaca gcaaaaccgt gaaccacctc acagtgacca ccaacggttt
    12961 gggtgagtgc atccgcaaag tcacggaggc ttccagattc cagcagcagc gaacgggatc
    13021 gtctctgggc aaaggttttg ggctgggatg cggttcttat ttgagcggtg cgggcctgcc
    13081 tgtttactgg aacaaaatgc cgcattccgg agtgcaaatc aaaatcgacc gcggtggcgg
    13141 cgtaactgtt ttttgcggaa gcacggacat cggtcaagga tcaaattctg tgctggccgg
    13201 aattgtggcc gaggaactgg gaatcgacat tgggaatatt attgtggtca cagcggatac
    13261 agatttaact ccggtcgatt tgggaagtta ttccagtcgt gtaacactga tgaccggaaa
    13321 tgcggccatt gacgcggctc gtcctttacg tgaaaaaata ttgtctgccg catccgaagt
    13381 tttgaaaatt ccggcagaaa aattaatgct ccggaatagt tctgttttat caaaaaacgg
    13441 ttccggagaa cctgtttcct ttgtggatgc tgtgcaatgg gcagaaacaa aatacggtat
    13501 gctgggttcc gtcggcagtt atacaccacc tagtcacggg ggaaaataca aaggcgcagg
    13561 tgtggggccg tccccgaatt acagctattc cgcttgtgtt gccgaagtca cttgtgatcc
    13621 ggaaagtgga atttatcgtg tggacaaact ctggatggca cacgatatcg gacaagcgat
    13681 caacgaaaaa ttggtcatcg gacaagtcga aggcagtgcg tacatgggtt tgagtgaggc
    13741 catgatggaa gaacaaattt atcgcaccgg aaaagacaac cccggaggtt tgcacaaaac
    13801 tccgtcgatg ttggattaca aaagtttgac cgcactcgat atgccggaaa tcgaaacatt
    13861 tttagtagaa acgcatgatc cggaagggcc gtacggtgcc aaagaagccg gccaagggcc
    13921 attactgccg atcatgcccg ccatcgccaa tgcgatttac aatgcggtcg gtgtgcgtgt
    13981 cgatgaagtg ccgattactc cggaaaaaat atatcaaggt ttaaaagagc taaaatctgg
    14041 aaagcctgga gggtccgggg ttattggaag agtcggccca aagaaatttc caaaaatcac
    14101 atttccggaa gccatcaaag tgaaatcggt tgacagttga cggttctgta tgcttctctg
    14161 tcaatttgac agagaataat tcgtgtctta attcgtgtcg taattaatta tgttgcgctt
    14221 accaccattt gaatatcaag ctccaaacag cattgctgag gctgttgcac tgaaagcaga
    14281 gtgcggtgac tcgtcgatgt ttgtttccgg aggaacagat ttgtttccga atatgaaacg
    14341 gcggcaattc acaccgaaaa tactgattgg tttgcggaaa ttagcagaac ttcgcagcat
    14401 tagtttggat gactccggaa atggattgat gattggttcc ggagtttctt tgagtgaagt
    14461 tgcggaacat ccggaaatcc tcaataattt tccggcatta gcaaaagcag catctttggt
    14521 ttctgcgcca cagttgcgga atatgggaac cattggcgga aatttgtgtt tggatacacg
    14581 atgtaattat tacaaccaaa catttccatg gcgcaaagca cttggatttt gtttgaaaaa
    14641 accggaaagt ccgatgcaaa acgatgctat ttgctgggtg gcgcggagca gtccaaaatg
    14701 tttggcggtt tcgtcctctg attgtgctcc ggtgatggtc gccttggatg cggaatttca
    14761 tctgatgaat cctgacggta agcgcattgt tccggcagga gaattttaca aaaatgacgg
    14821 aatggatttt ctcaacaaaa ctccggatga attgctggta tccattcacc taccgttaca
    14881 tgaaggatgg aaaatgaatt accgcaaatt aagtcggcgg ggtgcaattg attttccggt
    14941 gcttggcgtg gcagtagcat tgcgtttgga cacatccgga aattgtatgg aagcgcggat
    15001 tgttttgggt ggggtggctt cgtgtccact gcgggcgttt gaggcggaaa aacttttggt
    15061 gggtggctca ctcactgcgg aagggagcct gacacggatt gaaaatacgg ctcaagtagc
    15121 ggcaaaactg gcaaaaccga tggacaatac ggatattact ttgggcttcc ggaagaaaat
    15181 ggtgatgcag tttgtggcga aaacgattaa ggaagttttg aagtaacagg ttttagtcaa
    15241 gtaaatcaag taattccaaa ggtgcgttta tgattgcttg agcaccgttt tctctcagtt
    15301 ctttttccgg tcgaaatccc caaagcacgc ccaccggaaa acatccggca ctcgtggctg
    15361 ttttcatatc ggttgcggtg tctcccaaat aaagaaaaga cttcgctgga attttcaact
    15421 gctcgcaaat atcaatcatt ccagcaggat cgggcttgcg tggaatgcct tcccgttggc
    15481 ctaaaaccaa ttcaaattcc caatttggca aaagatgctt gactgcaagc aatgtaaatt
    15541 cgtgaggttt gttagacaaa actgtcagct tgagtttgcg ggattccaat tcatccaaca
    15601 atacaggaat gtcatcataa ggctgagtat gttcatccca gcactcacgg tatgctgtct
    15661 caaaatcctg tctgcatttt tctatgagag gttctttacg gacttcttca ggaagggcac
    15721 gggtaataag cattttcgct ccatctccta caaaatagcg aaatgcatca acggggtgtt
    15781 ctgggaatcc tctttttgaa agtattgtat ttgtacagtg tgctaagtcg gctaagctat
    15841 ctagcaatgt gccgtctaaa tcaaagcaga ttgcctgaaa ttgcatgaag ggcaatgaat
    15901 taattgttat tgttcaattc gtcacgccag attttagagg gatgaaatgt cacaaccttt
    15961 cgtgcagaaa tttcataatc cctttttgat tttggatcac gtccaggtcg agattttttg
    16021 ttacgcaact caaactttcc taaacctgtg atgagtacat cctcaccttt ctctaatttt
    16081 tctttgatca tttccaacat atcgttgacc actttttcag cttcaccaaa ctgtaagtca
    16141 gttcccagct ggatagcccg tactatatcg acttttacca ttcgctcctc cgatttgtct
    16201 aatatattaa tgctagcgat ttattaaaat tatttgttta taaatttttc tgactgactc
    16261 tcctccaaag acttctttta gctcttcctg aagctcggat tagcgaagac tgtaagaata
    16321 ctgagaccat actataacta tgggaagcgt cttctacaat acttttttta aaaaaaatca
    16381 attaaatcaa tttaaaatag aaaataagca caaaatgatt gaataaatca accttgatcc
    16441 ttctttaata tttttcaaag aagctcaaaa agttggacat tgtccagata ttttctctat
    16501 agtaagagtc acatcttccc agcagaaata tttatcagga taatttgtca atgtttttca
    16561 ggcagcacaa acgactgttt tttctagtaa ttattgtaat tgttctgtca attctcacct
    16621 tgtattttca gcaaattaag cttgaagaat tgcgcactga actggctaaa gtagaacttc
    16681 agaatgtaag ggtaggcgct ggaacttcaa aaggcaagtt gggaacagca atttttggtg
    16741 taattcaaaa taatggagaa cacacgatta agattgcaac tatgaatgtt aatttcatag
    16801 gcgaagatgg aaaatcttcc aaggttcata aatttttccc ggtaaataat tattctttca
    16861 gtgattcttt acctttggag cccggacagt caaaagagtt tggcttccct atcgatgaaa
    16921 ttattccgga agattgg
//
LOCUS       DTVD01000248            4678 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6362_length_4678_cov_472.791477, whole genome
            shotgun sequence.
ACCESSION   DTVD01000248 DTVD01000000
VERSION     DTVD01000248.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6362_length_4678_cov_472.791477"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="EYO68_09320"
     CDS             complement(<1..1656)
                     /locus_tag="EYO68_09320"
                     /inference="COORDINATES: protein motif:HMM:PF03961.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HIC08037.1"
                     /translation="MAKAELYKITINDGKVMLRIPEQLVGAETASMDDIQAELHLRNL
                     NYVPEQLLEIYNRTSGEFDYLADVETNDYTLQIELSEDESRAYVNIIPPSEEGDPLTM
                     ELIIAALEGKNIFQGISSKNIKNIIADKIFYEPALVASGKSVVHGKNGYPELLFIPEK
                     SRPALGTGVKLEEVTVLQKVEEGQELVRLMSATMGENGYSITGKLITAKSGKQYRIRP
                     GRNTRYNPEGTHIIATKSGVVCLNNDSISVEKIKVVDKVDASTGHLRFDGIVKIRGNV
                     ADRCSVEAVRIDIGGSVGKARLRSLGEIRVAHGIKGAIVQCGRSLHTSDMTDTQANVG
                     EHLLVDDFVLNSKVFCGSTLQITAPYGYVYGGVIQAGNLILLPNVGLPGTKETKSGKV
                     SEENSEPHTILEVGITTNNRKQFNELQKLTDTALQSLQDNLTEIDTILKELEAGGWDE
                     KKIETLSEFETKAEKNVISAFTNLRKRETHIEIKDLNRISNGGVVFITGKILNGTAIN
                     VRRSRFNVPVTSTDKAYSFDENGIKFNSCKELLKNYQQYFFRMP"
     gene            complement(1711..2448)
                     /locus_tag="EYO68_09325"
     CDS             complement(1711..2448)
                     /locus_tag="EYO68_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HIC08038.1"
                     /translation="MQNFHSAEPVRIQKVIAQAGIASRRAAEKLILAGEVRLNGETVL
                     KMGQKMLVGKDHLSVEGRRVKLPQRQKTQVYALYKPKNCITTLNDPEGRTTISDFFPR
                     TSARLFPVGRLDYDAEGLILLTNDGELAQQLMHPRHKVWKGYFVKVRGIVENAALSTL
                     RKGPVIEKRRHQPVRVKILHTVNDKTWLEVFLREGTNRQIKKMLQDLGYPVQKIKRFQ
                     VGTVSLGDLQSGESRALSPEEMKQLMN"
     gene            complement(2448..3188)
                     /gene="rnc"
                     /locus_tag="EYO68_09330"
     CDS             complement(2448..3188)
                     /gene="rnc"
                     /locus_tag="EYO68_09330"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIC08039.1"
                     /translation="MMKQEWIRLCQRVDYQFKKPEILRQALTHKSYLPVDLDEKFSNN
                     ERIEFLGDAVLDLVISEMLMEGFPELDEGGLSKFRASLVSESGLSKQAVTLELGECLL
                     LGRGEEMTGGRKKKSLLADTFEAVMAAIYLDSREEHGLSQITRVIRKIFLPHLPQDAE
                     GYITRDYKSELQEHVQKIMGIPSTYELVEESGPDHEKEFTMAVYVKEKEYGRGRGASK
                     KLASQLAAEKALLRIQEDTSFLQTEGTS"
     gene            complement(3190..3525)
                     /locus_tag="EYO68_09335"
     CDS             complement(3190..3525)
                     /locus_tag="EYO68_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08040.1"
                     /translation="MINLKFSFNPASILSLLIAFVLSVMLSGCYTVRVYHDTDMLREK
                     HDGEISSVSMLFTDSQIGGPAKISSACPGGASLIEIEQTVSDGLVHYLSLGFYSPQTI
                     RVWCKRRKR"
     gene            3669..4439
                     /locus_tag="EYO68_09340"
     CDS             3669..4439
                     /locus_tag="EYO68_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family deoxyribonuclease"
                     /protein_id="HIC08041.1"
                     /translation="MTSFIDTHCHLDILESTPEETIIEAKQAGVQRMVTIAVDEPSLD
                     FVSSTVQEFPAVYGSVGFHPHDASKLTKSLLQKIRQLAEEQHKLIAIGETGLDYHYMN
                     SPAEIQQQAFRKQLQLAVELNLPVILHSREAETDTLSILQEFPVPSLGVAHSFTSSIE
                     MAKILVDMGWYLGINGIVTFKNAEDLREVVRWLPLDRLLLETDSPFLAPIPFRGKPNK
                     PAHIPAIAGFIAKLRKISLEELAQQTTANAQRLFNFDE"
BASE COUNT         1229 a         1077 c          916 g         1456 t
ORIGIN      
        1 tggcatcctg aaaaaatatt gctgatagtt cttcaaaagc tctttgcagg agttaaactt
       61 gattccgttt tcgtcgaaac tataggcttt atcagttgaa gtcaccggca cattgaaacg
      121 tgaacggcgt acgttgattg ccgttccgtt caagattttg ccggtaatga aaaccacacc
      181 tccatttgaa attctgttca agtcttttat ttctatgtgg gtttcccgtt ttctgagatt
      241 tgtgaaagca ctaataacat ttttttcggc ctttgtttca aattcactca gggtttcaat
      301 ttttttctca tcccatccgc ctgcctcaag ctccttcaga atagtatcaa tttccgttaa
      361 attgtcctgc agagactgca atgccgtatc agtcagtttt tggagttcat taaactgctt
      421 tcggttgttt gtcgtaatac caacctctaa aatagtatgg ggctcactgt tttcttcact
      481 aactttgccg cttttagttt cttttgttcc aggcaaacca acattcggca gcaggataag
      541 atttccggct tgaataactc ctccgtaaac ataaccgtat ggtgccgtga tctggagagt
      601 tgaaccgcaa aaaacttttg agttcaggac aaagtcatct actaaaagat gttctccgac
      661 atttgcctga gtgtcagtca tgtcgcttgt atgaagcgat ctcccgcatt gaacgatggc
      721 acctttgata ccatgtgcga ctcggatttc accgagcgag cgtagtcgtg cttttcctac
      781 tgaacctcca atatctatcc gtactgcttc tacgctgcag cgatcagcaa cattaccccg
      841 tatttttaca atcccgtcaa aacgcagatg tcctgtggag gcatcaactt tatcgactac
      901 ttttatcttt tccaccgaaa tgctgtcgtt attgagacag acaactccgg attttgttgc
      961 aatgatatga gtaccttccg gattatacct ggtgtttcgt cccggacgga tacgatattg
     1021 ttttccggat ttggcagtaa tcagctttcc ggtaatacta tagccgtttt cacccattgt
     1081 ggccgacatc aaccgtacca gttcctgtcc ttcttctact ttctgtaaaa cagttacctc
     1141 ttcaagtttt accccagttc ccaaagcagg tcgagacttc tcgggaataa aaagcagttc
     1201 cggataccca ttttttccat gtacaaccga tttgccgctg gcaactaatg caggttcata
     1261 aaaaatctta tctgcaataa tatttttaat atttttgctg ctgattccct gaaaaatatt
     1321 tttcccctcg agcgcagcaa taataagctc catcgtcaga ggatcgcctt cttcacttgg
     1381 aggaatgata tttacatagg cccttgactc atcttcactg agttcaattt gcaaagtgta
     1441 atcattggtt tcaacatccg ccaggtaatc aaattcaccg cttgttcgat tatatatttc
     1501 cagcaattgt tccggaacat agttcaagtt cctcaggtga agttctgcct gaatatcatc
     1561 catgctggca gtttctgctc ccaccagctg ctctggaatg cgcagcatca ctttaccgtc
     1621 gttaatcgta attttatata attcagcctt ggccattgtt tgagtgtaga atgttgggtc
     1681 ttgagtggag ggtggtgttg actttcggac ttaattcatt aattgtttca tttcctccgg
     1741 actcaaagct ctgctttcgc cggactgcaa gtcacctaaa gaaaccgttc caacctggaa
     1801 gcgcttaatt ttttgcaccg gataacctaa gtcttggagc atttttttga tttgccggtt
     1861 tgttccttcc ctgagaaaga cctcaagcca tgttttatcg tttacagtat gcagaatttt
     1921 tacccgtaca ggctggtgcc tgcgtttttc aatcaccggt cctttccgca aagttgacag
     1981 agctgcattc tcaacgatac cacgtacctt aacaaaataa cctttccaaa ccttgtgccg
     2041 gggatgcatc agctgctggg ccaattcacc gtcgttggtc agtaaaatca agccttctgc
     2101 atcataatca agccgtccta caggaaaaag cctggctgaa gttcgaggaa aaaagtcact
     2161 gattgtggtg cgtccctcag gatcgttgag ggtcgtgatg caatttttag gcttgtaaag
     2221 agcatatacc tgcgtttttt gacgctgcgg taattttacc cgccgtcctt ccacgctcaa
     2281 gtggtctttt ccaacgagca ttttctgtcc catcttcagg acagtttcac cattcagcct
     2341 cacttcgccg gccaaaatta atttttctgc agcacgtctt gacgcaattc cggcctgggc
     2401 aataactttt tggatccgta caggctctgc tgaatgaaaa ttttgcatca gctggtacct
     2461 tctgtttgaa gaaaagaagt gtcctcttgt atcctcaata aagctttttc tgcagcaagc
     2521 tgactggcta gttttttgct ggcacctcgc cctcgtccgt attctttttc tttgacataa
     2581 actgccatcg taaattcctt ttcatgatca ggaccagatt cttcaaccag ttcatatgtc
     2641 gagggaattc ccattatttt ctgtacgtgt tcctgcagtt ctgacttata atctcgtgta
     2701 atataaccct ctgcatcttg cggcaaatgt ggaagaaaaa ttttgcgaat tactctggtg
     2761 atttgggaga gtccgtgttc ttcacggctg tcaaggtaga tagctgccat aactgcttca
     2821 aaggtgtcag ccagcaaaga ttttttcttt cgccctcctg tcatttcttc acccctgcca
     2881 agtaacaaac attcgcctaa ttccagggtc acggcctgtt tgctgagtcc ggattcactg
     2941 accaggcttg cccggaattt tgagagtcct ccctcatcga gttccggaaa cccttccatc
     3001 agcatttcac tgatcaccag gtccaggact gcatcaccaa gaaactcaat cctttcgtta
     3061 ttactaaatt tttcatctaa atccactggc agataagatt tatgcgtcag tgcttggcgg
     3121 agaatttccg gttttttgaa ttgataatca acacgttgac ataagcggat ccattcttgt
     3181 ttcatcattt tacctttttc ttcgtttgca ccagacgcga atggtttgcg gactgtagaa
     3241 tccaaggctc agataatgca ccagaccgtc tgaaacagtt tgctctattt cgataagtga
     3301 agcacctccg ggacaggctg aactaatttt tgccgggccg ccgatttggc tgtctgtgaa
     3361 tagcatactg acgctggaaa tttccccatc gtgcttttca cgcagcatgt ctgtatcgtg
     3421 ataaacacgt acggtataac agccggaaag cataaccgaa agtacgaaag caatcagcaa
     3481 tgataagatg gacgcaggat taaagctgaa ttttaagttg atcatttgcc aaaattttga
     3541 aaggttgcag agcagcaaac ggatttctgc tgtgagttca gagtcaaaac tgacggcttc
     3601 gacattacaa acagcaagca aattcttaaa attattatca atacaaatct atcccattta
     3661 tacactctat gacaagtttt attgatactc actgccacct tgacatcctg gaatcaactc
     3721 cggaagaaac aattattgaa gcaaaacagg caggtgttca aaggatggtt actattgctg
     3781 tagatgagcc atctctggat ttcgtctcaa gtacagtaca ggaatttcct gcagtttacg
     3841 gaagtgttgg ttttcatccg catgatgctt caaaacttac aaagtcgcta ttacaaaaaa
     3901 ttcggcaact tgccgaagaa cagcataaac tgattgcaat tggcgaaact ggacttgatt
     3961 atcattatat gaacagtcct gctgagatcc agcaacaggc gttccgaaaa cagcttcaac
     4021 tggcagttga gctcaatctc ccagttattc tgcacagcag ggaagcagaa accgacaccc
     4081 tgagtattct gcaggagttt ccggtaccat ctcttggtgt ggcacacagt ttcaccagtt
     4141 caattgaaat ggcaaaaata cttgtggata tgggatggta tctgggcatt aatggaatag
     4201 taacttttaa gaatgccgaa gatttacgag aagtggtgcg ttggctgcca cttgatcgtt
     4261 tacttctgga aacagattca ccctttcttg ccccaattcc ctttcgtgga aaacccaaca
     4321 aaccggcaca cattcccgcc attgccggtt ttattgctaa gttgagaaaa atttccctgg
     4381 aggaattagc acaacaaacc acagcaaacg cacagcgctt gtttaacttt gatgagtgaa
     4441 tggtgctgca ttcttaataa agtcctgtat cgataatcct tccataatat taaataaaat
     4501 cctgtaaaaa caacaatctg cgtctataat aattgaaaat ttatttgcgt ttattgattt
     4561 cttaactggc atgcaaagat gcagaaaaat ttttccactt tttgttaaac caggaaattt
     4621 atgtggtgat aatatgatgt tccagaatta attcagacca actgtttgaa ttttcaca
//
LOCUS       DTVD01000249            4634 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6480_length_4634_cov_188.882507, whole genome
            shotgun sequence.
ACCESSION   DTVD01000249 DTVD01000000
VERSION     DTVD01000249.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_4634_cov_188.882507"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="EYO68_09345"
     CDS             complement(<1..1284)
                     /locus_tag="EYO68_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC08042.1"
                     /translation="MNQRDPRYDILFEPVQIGPLTARNRFYQVPHCNGMGYMRPNTLA
                     AMRGIKAEGGWAVVCTEEVEIHANSDNGSAAEGRLWDDGDIPALAKMTEAVHEHGALA
                     GIELVHGGYSSANNYSRIPPLAPSAISVNSYDPVQARAMNKADIRDFRKWHRQAALRA
                     KQAEFDLVYVYAGHSLTLLMHFLSRRFNHRTDEYGGSLENRVRLFREVLEDTKDAVGH
                     RCAVAVRFAVDELLGPKGITSENEGREVVEMLAEIPDLWDVNISEWENDSATSRFEKE
                     GYQEPYIDFVKSLTTKPVVGVGRYTSPDTMVSLIKRGVMDMIGAARPSIADPFLPKKI
                     EEGRVDEIRECIGCNICVAGDWLSVPMRCTQNPTMGEEWRRGWHPERIEPRESDDGVL
                     VVGGGPAGLECALALGKRGYQVILAEESEELGGRVS"
     gene            complement(1291..2682)
                     /locus_tag="EYO68_09350"
     CDS             complement(1291..2682)
                     /locus_tag="EYO68_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIC08043.1"
                     /translation="MRLEKIEEIQSFDNKNFVHPWEYMKDVGQKKRMFAEKAEGIYLY
                     DENGEKLIDGPGGMWCVQIGYGRPEMAKAISDQILKVPYYSPWNMSSSPSAMLAHKIA
                     ERTPGDLNHVFFTTCGSTAVDSAIRFVHFYNNVLGRNKKKKIISREKSYHGSTYLSAT
                     MCGKDRDKDWMDTAKELVHFLPDVNPSRRPEKISVDEFLEEKISDLENAILTLGAENV
                     GAFIAEPVLASGGVIVPPKGYHQRCLEVCRKYDVLYISDEVVTAFGRLGHWFASKEVF
                     GIEPDIITCAKGLTSGYIPMGACIISDRIFSQISGDGSQYATFSNGYTYSGHPVSSAA
                     ALKNIEIIENEGLLEHVREISPYFQQRIKELRKLPLVGNTRGVGLVGCVECQIVDSES
                     HDALEMDIAMGSRIDKHCQALGLIVRPLWNMCVFSPPLVITMEQIDEMFNILEKGIIL
                     TTDELSKEGLWKG"
     gene            complement(2701..4287)
                     /locus_tag="EYO68_09355"
     CDS             complement(2701..4287)
                     /locus_tag="EYO68_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019296992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase"
                     /protein_id="HIC08044.1"
                     /translation="MVDVERVGRLGRVRTGGRAGRKAQRSSENQVPVVQAGMTGGAYK
                     PLQDTDLEKIHQAALEILATLGIAEATPEVIELATECGCIVSDFGRLCFPKALIEDVL
                     VKAANEYVVHSRNSAHADLHVGGYRVHYATSGEAVTIFELDSKTYRPSTLLDLYDACR
                     LVDKLEYIHQFGQTVIPTEISDLDEHDFNVAYALVAGTEKPFEMTFNRAQNIKGAVKM
                     FDMVLGGEGRFAEKPFCTFGGCPIVSPLRFAKENLDVLVESSRLGLINDIAIAPQAGA
                     TAPATLAGTLAQVTAEGLACLAIVNFINPGCPMSFAIWPFISDLRTGSFSGGSGEEAL
                     IIAAAVQIGKFYNLPTTVPSCMTDSKIPDAQAGYEKGISSVLAGLAGGNRILESAGML
                     GSLMGCSFEALVIDNDMLGMVQRVIRGIEVTEETLSVEVIKDVALGAGHYLGHAQTLD
                     RMQSEFLYPKIADRAPPGVWEQEGSKDILERAHETAFEILSTHFPDHITPKADTNIRD
                     RFPILLSRDAMKPSARCKKG"
BASE COUNT         1153 a         1227 c         1019 g         1235 t
ORIGIN      
        1 gctgacccgt ccgccaagtt cttctgactc ttctgcgaga atcacctggt agccacgttt
       61 gcccagtgct agagcgcatt ctaatccggc aggacctccg cccaccacca gtacaccatc
      121 gtcgctttcg cgaggctcga tcctctccgg atgccagcct cgacgccatt cttctcccat
      181 cgttggattt tgagtacatc gcattggtac tgacagccag tctccagcta cgcagatgtt
      241 gcagccgata cactcacgga tttcatcaac acgtccttcc tcaattttct tcgggaggaa
      301 cgggtcggca attgatggcc gcgccgcacc gatcatgtcc atcactccgc gtttaatcaa
      361 tgagaccata gtgtccggag aagtgtagcg tcccacaccc accacgggtt tggttgtgag
      421 tgattttaca aaatcgatgt atggttcctg atatccctct ttctcaaagc gggaagttgc
      481 tgaatcgttt tcccattcac tgatattaac gtcccaaagg tcagggattt ccgcgagcat
      541 ttccacaact tcccgacctt cattttctga ggtaatgccc ttgggtccta gaagttcatc
      601 gactgcaaaa cgcacagcaa cggcacaacg gtggcctacc gcatcctttg tgtcttcaag
      661 tacctcccgg aagagtcgca cccgattttc caagctgcca ccatattcat ctgtacgatg
      721 attgaagcga cgcgaaagga agtgcataag cagggtaagg ctgtgtccag cgtacacata
      781 aacgaggtcg aactccgcct gcttggcacg caacgcagcc tgacggtgcc acttcctaaa
      841 gtcacggata tcggctttat tcatcgcccg tgcttgtacc ggatcatagg agttgacgga
      901 gattgcactg ggcgcaagcg gtggaatccg gctataattg ttggcgctgg aatagccgcc
      961 atgcaccaac tcgattccgg ccaacgcccc atgctcatgt acggcctctg tcattttagc
     1021 cagtgccgga atgtctccat cgtcccacaa ccgcccctct gctgccgagc cgttgtcaga
     1081 gttggcgtgg atttccacct cttccgtgca gaccacagcc catccacctt cggccttgat
     1141 cccccgcatc gccgccaacg tgttggggcg catatagccc ataccgttgc agtgtggaac
     1201 ctgataaaag cgattcctcg cggttagcgg cccaatctgt acaggttcaa aaagaatgtc
     1261 gtaacgagga tcacgttggt tcatcttgtg ttatcccttc cacagtcctt ccttactcaa
     1321 ctcatctgta gtaaggatga tacctttttc caggatgttg aacatttcat cgatttgttc
     1381 catagttata accaatgggg gtgagaacac acacatgttc cagagcggac gtacaatcag
     1441 gcccagtgcc tgacagtgtt tgtctattcg ggaccccatt gcaatgtcca tttcaagtgc
     1501 gtcatgactc tccgaatcaa caatttggca ctctacacag ccgaccaatc ctactccgcg
     1561 tgtgttgcca acaagaggca gttttcttaa ctccttgatg cgttgctgga aatatggaga
     1621 gatttcacga acatgctcca gcaaaccctc gttttcgatt atctctatgt ttttgagggc
     1681 agcggccgaa cttactggat gtccagaata agtatagcca ttggaaaacg tagcgtattg
     1741 agaaccatct cctgagattt ggctgaaaat tcgatcggaa atgatgcagg ctcccatcgg
     1801 tatgtagcca gaggttaacc cctttgcaca agtaataatg tcgggctcaa tcccgaacac
     1861 ctctttagag gcgaaccagt gtcccaaacg tccaaaagca gtcacaacct catcagaaat
     1921 gtatagtaca tcatacttgc ggcagacctc caggcaacgc tggtgatagc ctttgggagg
     1981 cactattaca cctccagaag ccagtacagg ctcagcaatg aatgccccga cattttcagc
     2041 tccgagggtc aggatagcat tttctaagtc agaaattttt tcctccagga actcatcaac
     2101 actaattttt tccggacggc gacttgggtt gacatccgga aggaagtgaa ctaattcttt
     2161 tgcggtatcc atccaatcct tgtcgcgatc ttttccacac atagtcgcac tgaggtaggt
     2221 gcttccgtgg taggattttt ctctagaaat aatctttttc tttttatttc ttcctaaaac
     2281 attgttataa aaatgcacaa agcgaatcgc actgtctacc gctgttgaac cacaagtggt
     2341 gaagaagaca tggttcaagt cccctggagt gcgctctgcg atcttatgcg ccagcatggc
     2401 acttggagaa gaactcatgt tccatggcga ataatacggt accttaagga tctgatcaga
     2461 aatggccttg gccatttctg ggcgtccgta gccaatctgc acgcaccaca ttcctccagg
     2521 gccgtctatc aatttttcac cattctcatc gtagagataa atcccttctg ctttttcggc
     2581 aaacatgcgt tttttttgcc ctacatcttt catgtattcc catggatgta cgaaattctt
     2641 gttgtcaaaa ctttggattt cttcaatttt ttcaagtctc atttttagtt tcctaaacag
     2701 ttagcccttt ttacagcggg cagagggttt catggcgtca cgtgataaca aaatcgggaa
     2761 tcgatcacgt atgtttgtat cggctttggg agtgatgtga tccggaaagt gagttgataa
     2821 aatttcgaaa gctgtttcgt gagcacgttc gaggatgtct ttagaacctt cctgctccca
     2881 tactccggga ggggcgcgat ccgctatttt aggatacaaa aactcgctct gcatccggtc
     2941 aagtgtttgg gcgtgtccga gatagtgccc agcccctaaa gcgacatcct taatcacttc
     3001 aacactcaga gtttcttcgg tgacttcaat cccacgaatg acccgttgta ccatgccgag
     3061 catgtcatta tcgatcacga gtgcttcaaa ggaacagccc atcaagctcc ccagcatccc
     3121 cgcggattcc aggatccggt taccgcccgc taatccagcc agcacggaac ttataccctt
     3181 ttcataaccg gcctgggcat ccgggatttt tgagtctgtc atgcaggaag gtactgtcgt
     3241 cggcaaattg taaaattttc cgatttgtac agctgcagcg ataatgagtg cctcctcgcc
     3301 gcttcctcca gagaaagaac ctgtacgcaa atcgctgatg aatggccaga tagcaaagct
     3361 catcggacag ccgggattga tgaaattgac aatcgccaaa catgccagtc cttctgcagt
     3421 cacttgggcc agtgtcccgg ctaacgttgc aggagctgtt gctccggctt gaggtgcaat
     3481 tgcgatgtca ttgatcaacc caagcctact gctttccacc agcacgtcca ggttttcctt
     3541 ggcaaatcga agtggtgaga caattggaca tccgccaaaa gtacaaaatg gtttttcagc
     3601 aaaacgacct tctcccccca aaaccatgtc aaacattttc actgcccctt tgatgttttg
     3661 agcacggttg aaggtcattt caaatggttt ttccgttccg gcaaccaatg cgtaggccac
     3721 attaaaatcg tgttcatcta aatccgaaat ttcagtgggt attacggttt gcccaaactg
     3781 gtgaatgtat tccaacttgt ccactaaccg acaggcatcg taaaggtcaa gcagtgtgga
     3841 agggcgatag gtttttgagt cgagctcaaa gatggtcacg gcttctccgc tggtggcgta
     3901 atgcacccga tagccgccga cgtgcaagtc ggcatgagca gaattgcgac tatgcacaac
     3961 atattcgttg gcggctttca ccaagacatc ctcaatcaag gcttttggaa agcataatcg
     4021 tccaaaatca ctgactatgc atccgcattc tgtcgccagt tcaatcactt ccggggttgc
     4081 ttcggcgatt cctaaggttg ccaaaatttc cagtgcagcc tgatgaatct tctccaaatc
     4141 cgtatcttga agcggtttgt atgcccctcc ggtcatccct gcctgtacca ccggcacttg
     4201 gttttctgaa cttctctgag ctttccgtcc agcacggcca cccgtacgaa ctctcccgag
     4261 ccgccccact ctttcaacat ctaccataaa atttcttaat tccggtggag cttaacccaa
     4321 ccagactgtt ctatatgtac tgcaattagc tggaaatctt catgacaacc cgtatttatg
     4381 tttggggagt tattcccgat atctcaaata ggacatcatc aaggctggcc aatcatgaaa
     4441 gtaaaagaga aggcatcaaa aagcaatgag aaagagctag tttcagacta cacgaaactg
     4501 gttttaggag agtacaactt tataagtcaa acccggattc aatagttgat ctcttcacag
     4561 caggcttcct aacggttttc gaaataagat agatgataat tcaaaatcta gcaagatcaa
     4621 gtaatattac aata
//
LOCUS       DTVD01000250            4626 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6501_length_4626_cov_520.706848, whole genome
            shotgun sequence.
ACCESSION   DTVD01000250 DTVD01000000
VERSION     DTVD01000250.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6501_length_4626_cov_520.706848"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="EYO68_09360"
     CDS             <1..372
                     /locus_tag="EYO68_09360"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508846.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIC08045.1"
                     /translation="TRSGAKAEGRKVKGIIHWVSIQHSVPVEVRLYDRLFVNPSPGSD
                     DPGGNFLNDLNPDSIETVTSCRVEPGLLKAKPADVYQFERVGYFCVDSKESRPGALVF
                     NRTVTLRDTWAKLEQEHTGQN"
     gene            333..1640
                     /locus_tag="EYO68_09365"
     CDS             333..1640
                     /locus_tag="EYO68_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08046.1"
                     /translation="MGQVGTGAYRAKLRGMKKMVIYRLQKFSTFAFLIMVSFSLLSGP
                     GSSKVQAFDDASEIHHSYLNQENFLDIKSYQFHKLKEDEWFESPGGWRLAGGSMGLDL
                     LYSHLEVRLPHALSDETTVFFRAWQEEFYEIKPFRYLVEVEWRPNEWAAFSLLGMPEY
                     DKRKADQGASITLGKRPWNFLRFQQLFQDLFYNEKNFYDNSYYSPHPMENNLEGSFNW
                     KNWRARFSYVLDKPFKQVFPKQSLTFTYNGKDSSAVLDYHYGHQRIAGLSWRSFDIRK
                     RRETTQSTETASPDNREQQLLYSSADFYWLHPLSTTMHGTLGLREDHFRNYFRQLDLD
                     YDSYDFHLWTLQLYGILRHQTATDRFWEYGLYVGDTDKVTDYLNAARTDKTRRKHEAK
                     LRVSKEFRYLSNNSALMFTTSWNIDDFFNDFWDGGNISYQRTF"
     gene            complement(1651..2451)
                     /gene="folP"
                     /locus_tag="EYO68_09370"
     CDS             complement(1651..2451)
                     /gene="folP"
                     /locus_tag="EYO68_09370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIC08047.1"
                     /translation="MGILNSTPDSFSDRSRFFKLDDALEQARKMINDGADWLDIGGES
                     SGPDSQDVPLDEELQRVIPLIQKVRMESDIWISVDTWKAEVARQSLEAGADAINDVTA
                     LRGDPELAKVVKEFQVPLVMMYSKDATPRTTRQQPDYEDVMDTIKSFFRERLQFAESN
                     GILNKQIVLDPGMGFFISGTPKYSFEIIRRISELHEFDLPLLVGPSRKSFLAGVTPGR
                     TLNFSERDIPAAVVSSIALWQGVSMLRFHEVEQGRLLIDTIEALKSSA"
     gene            2631..3503
                     /locus_tag="EYO68_09375"
     CDS             2631..3503
                     /locus_tag="EYO68_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08048.1"
                     /translation="MLSSGLGKYVAPTALGAGYAIAKMFSLRALDTELAIAQDFTYQF
                     LAGVLLGFALRPVTNMIYWKWTTSIVFFSIFLMTFGPFGQILKRLVWGIPFDNTFWLL
                     LIPEVIASLTVGILATLLIPSQHQVIGLNFLRRRLQKEISISMLLKLLGCGLIYALLF
                     LVFQITFNESFSAPFWLGRIEEFLALPALSAQSKILLLWGQGILNTLVILPLFLVFFR
                     EKMELIVVFGSLAFVVAEFSPAFANFQLIEPLLLIDQVFIGFCLQFLFVVCTVFCFGR
                     NVFNKTEQIFREKP"
     gene            3530..>4626
                     /locus_tag="EYO68_09380"
     CDS             3530..>4626
                     /locus_tag="EYO68_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC08049.1"
                     /translation="MNLFAERNSWIDTENAFKIGPHIVHVEQQKGAVIKLNLGEPDFS
                     VPKHIKDEIKRQLDLDNTHYCDPKGLLSLRTAISNQVNETRGLQTNPEQVVVFPGGKP
                     SIGFAQQVYCNPGDEVIYPSPGFPIYESFIRYVNAIPVPLHLDESENFTFSPADLAHK
                     ITAKTKLIYINYPSNPTGGVASKSLLEETARIILERCQPDVRIYSDEIYEKIIFDGQQ
                     HHSISSIPEMTERTIIASGFSKTYAWTGGRIGYAVFPTVEEAEVFKRLNINYFSCVPP
                     YNQEAARVALESPLSKQSVKKMVETFESRRNLILQQINEIPGISCQKPGGAFYLFPNI
                     SGICENLGLIEKHHSLSAEEQSSPANIFQMFA"
BASE COUNT         1312 a          982 c          979 g         1353 t
ORIGIN      
        1 actagatccg gagcaaaagc tgaaggacgc aaggtaaagg gtatcatcca ttgggtctca
       61 attcagcatt ctgtacctgt tgaagttcga ttgtatgacc gactgttcgt taatccttcg
      121 ccggggagtg atgatccggg tggtaatttt ctcaatgacc taaatccgga ttccattgag
      181 accgtaacat cctgccgtgt cgaacctgga ttactgaaag ccaaacctgc agatgtttat
      241 cagtttgaac gtgttggtta tttttgtgta gattcaaaag agtcccgacc gggagcttta
      301 gtctttaacc ggacagtaac cctgcgggac acatgggcca agttggaaca ggagcatacc
      361 gggcaaaact gaggggcatg aaaaagatgg tcatttacag actgcagaaa ttttcaactt
      421 ttgctttttt gattatggtc tctttcagtt tattatccgg accaggctcc tcaaaagtac
      481 aggcattcga tgatgcttca gaaattcatc attcctacct caatcaggaa aattttcttg
      541 atattaagtc ctatcaattt cataaattaa aagaagatga gtggtttgaa agtcccggag
      601 gttggaggct ggccggcgga agtatgggac tggacctgct ttattctcac cttgaggtac
      661 gcttgcccca cgcattgtca gacgaaacta ctgttttttt tcgtgcatgg caggaagaat
      721 tttatgaaat aaaacctttc cgttatctgg tcgaagttga atggcgtccg aatgagtggg
      781 cagccttttc cctgcttgga atgccggaat atgataagcg aaaggcagat cagggtgcaa
      841 gcatcacgct gggaaaacgt ccttggaatt tcctgcgttt ccaacagttg tttcaggatc
      901 tgttttacaa cgaaaagaat ttttacgata attcgtacta ttctccacat ccaatggaaa
      961 ataatctgga aggttcattc aactggaaaa attggcgtgc acgcttcagt tatgtattag
     1021 ataaaccttt caaacaagtt tttccgaagc agagcctgac attcacatac aatggcaaag
     1081 acagcagtgc tgtgctcgat tatcactatg gacaccaacg gattgccggg cttagctggc
     1141 gcagctttga tatccgcaaa cgccgggaga caacacagtc cactgaaaca gcctcacctg
     1201 ataatcgtga gcagcaactg ctttacagct ctgcagattt ttattggctt catccgctga
     1261 gtacaacgat gcacggaaca ttaggtctgc gagaggatca ctttcgcaat tattttcgtc
     1321 aactcgatct tgactacgac agttacgatt ttcatttgtg gaccttgcag ctttacggaa
     1381 tcctccgtca ccaaactgca acggacaggt tttgggaata tggactgtat gtaggagaca
     1441 ccgataaagt cacggattat ctaaacgcag caaggacgga caaaacacgc cgcaaacatg
     1501 aagccaagct ccgggttagc aaggaattcc ggtatctgag caacaactct gcgttgatgt
     1561 tcacaaccag ttggaatata gacgactttt ttaacgattt ttgggatgga ggaaatattt
     1621 cttaccaacg gactttctag tttataacat tcatgcgctg ctttttaaag cctcaattgt
     1681 atcgataagc aggcgtccct gctctacttc atgaaaacgc agcattgaaa ctccctgcca
     1741 gagagcaatg ctggaaacga ctgcagcggg gatgtcccgc tctgaaaaat ttaaggtgcg
     1801 tcccggagta acaccggcaa gaaacgattt cctggaaggt ccgaccagca aaggcaaatc
     1861 gaattcatga agttctgaaa ttctccggat gatttcaaaa ctgtattttg gagtaccgct
     1921 gataaaaaac cccatgccgg gatccagcac aatttgttta ttcagaatgc cattagactc
     1981 agcaaactga agacgttctc tgaaaaaact cttgatggtg tccatcacat cctcgtaatc
     2041 tggttgctgc ctggttgttc gcggtgttgc gtctttggaa tacatcataa ccagtggtac
     2101 ctgaaattcc ttaacaactt tggccagttc aggatcccct cgcagagcag tcacatcatt
     2161 gatcgcgtct gcaccggctt ccaaagattg acgtgcaact tctgctttcc aggtgtccac
     2221 tgaaatccaa atgtcgcttt ccatgcgaac tttttgaatg agaggaatta cacgctgaag
     2281 ttcttcgtcc aatggcacat cctgactgtc cggaccagaa gattcgccgc caatgtcaag
     2341 ccagtctgcg ccgtcattaa tcatttttcg ggcttgttcc aatgcatcgt caagcttgaa
     2401 aaaacgagag cggtcagaaa atgaatccgg agtggaattc aggattccca tcaggcacgg
     2461 ctttcccaga tttaattttt tagctctgga gactaaagta cgcggcttta atataattga
     2521 ttttgaaatc atctttcttt atttacgttg gtgccaacaa taaaataatg atactccatt
     2581 acaaagccta tttccaaaaa tattcaaatt aataaactga acatttgctg atgttatctt
     2641 ccggacttgg aaaatatgtg gcgccaactg cactaggtgc aggatatgcg attgcaaaaa
     2701 tgttttctct gcgtgcactt gatacagaat tggctattgc tcaggatttc acctaccagt
     2761 tccttgcagg agtgttactt ggttttgcct tacgtcctgt aacaaatatg atctattgga
     2821 aatggacgac ctcaattgtc tttttttcaa tttttttaat gacatttggc ccatttgggc
     2881 aaatattgaa acgccttgtt tggggtattc catttgataa tactttttgg ttgttgttaa
     2941 ttcccgaagt aattgcgtca ttaacagtcg gtattttagc aactctattg atcccttctc
     3001 agcatcaggt catcggtctc aattttctcc ggcgcagatt acagaaagag atcagcatct
     3061 ccatgctgct aaaactgctc ggctgcggtt taatttacgc actcttattt ttggtttttc
     3121 aaatcacatt taacgagagt ttttctgcac cattctggtt agggcgtata gaagagtttt
     3181 tagcattacc ggctctgtcg gcacaatcaa aaatccttct tttgtgggga caaggtattc
     3241 taaatactct ggtaattcta cctttattcc tggttttttt ccgtgagaaa atggaactga
     3301 ttgtggtctt tggttcattg gcttttgtag tagcagaatt ttcaccagct tttgccaatt
     3361 tccagctaat tgaaccactg ttgctgatcg accaggtttt cattggattt tgtctgcagt
     3421 tcctgtttgt agtctgcaca gttttttgtt ttggacgtaa tgtgtttaac aaaacagaac
     3481 aaattttcag agaaaaacct taacctctaa attaacaacc ggacaacaaa tgaatttatt
     3541 tgcagaacga aactcatgga tcgacacaga aaatgcattc aaaattgggc cgcatattgt
     3601 tcatgtagaa cagcaaaaag gagcggtgat aaaattgaat ttgggagaac cggatttttc
     3661 tgtaccaaaa catataaagg atgaaataaa gcggcagttg gatcttgata atacacatta
     3721 ttgtgatccc aaaggcctgc tttctttacg aacagcaatc agcaatcagg tcaacgaaac
     3781 acgaggactg caaacaaatc cggaacaggt ggttgtcttt ccgggtggta aaccttcaat
     3841 tggttttgca cagcaggttt actgcaatcc tggagacgag gtgatttatc caagtcccgg
     3901 attcccgatt tatgaatcgt tcatccgtta tgtcaatgcg attccggtcc ctctgcatct
     3961 tgacgaatct gagaatttta ctttttctcc tgcagatcta gctcacaaaa taacagccaa
     4021 aacaaaatta atttacatca attatccatc caatccaact ggtggagttg cctcaaaatc
     4081 cctgcttgaa gaaacggctc gaataatttt ggagcgttgc cagccggatg ttcgaattta
     4141 ttcagatgaa atttacgaaa agattatttt tgacggtcaa caacaccact ccatttcctc
     4201 aataccggaa atgacagaac gcacaataat tgccagtggt ttttcaaaaa cttatgcctg
     4261 gaccggtggg agaatcggct atgcagtttt tccaacagtt gaagaggcag aggtttttaa
     4321 aagactgaac attaattatt tctcctgcgt tccgccctac aatcaggaag ctgccagagt
     4381 tgctctggaa agtccacttt caaagcagtc cgtgaaaaaa atggtagaga cttttgaatc
     4441 acgcagaaat ttgattttgc agcaaataaa tgaaattccg gggattagct gtcaaaaacc
     4501 aggaggggca ttttacttgt tcccaaatat ttcaggaatc tgtgaaaatt taggcctgat
     4561 tgaaaaacac cacagccttt cggcagaaga gcaaagttct ccggcaaaca tcttccaaat
     4621 gtttgc
//
LOCUS       DTVD01000251            4620 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6517_length_4620_cov_169.547645, whole genome
            shotgun sequence.
ACCESSION   DTVD01000251 DTVD01000000
VERSION     DTVD01000251.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_4620_cov_169.547645"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="EYO68_09385"
     CDS             complement(<1..309)
                     /locus_tag="EYO68_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC08050.1"
                     /translation="MKIKDIYETMEYSPAPESPDLALEWLKEHKSKFGLFINGKWCKA
                     KSGKVFSTDNPANGKKLASISEAGKSDVDSAVSAAKRAFPMWKALSGHERARYLYALA
                     R"
     gene            complement(360..1253)
                     /gene="deoC"
                     /locus_tag="EYO68_09390"
     CDS             complement(360..1253)
                     /gene="deoC"
                     /locus_tag="EYO68_09390"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIC08051.1"
                     /translation="MNRSAIERRCATLTKRRSIKKEWQAAWLLKSLRCMDLTTLSSDD
                     TPDRVNRLCAKARQPLRPELIENLCLNGLKPRVGAVCVFHAYVETAVNALKNSGIPVA
                     AVSTGFPHGLNSMPRRIEEIKDSVAAGAKEIDIVITRSHVFAGDWQALYNEISAFRKA
                     CGESHLKTILGTGELGTFRNVAKASLVCMMAGADFIKTSTGKESINATLPVSLVMIRM
                     IREYLEITGSYVGFKPAGGIRNAKQSLEWLILMKEELGNKWLEPDLFRFGASGLLTDI
                     ERQLEHFVTGRYSAAHRQPTA"
     gene            complement(1504..1740)
                     /locus_tag="EYO68_09395"
     CDS             complement(1504..1740)
                     /locus_tag="EYO68_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIC08052.1"
                     /translation="MRYQVKLTKSEEGVAVSCPGLPGCWSQGATEEEDLENIQDAIVD
                     YLSVVDELTQESETRYVEVARDAKTFGSISQKSD"
     gene            complement(1752..2678)
                     /gene="rbsK"
                     /locus_tag="EYO68_09400"
     CDS             complement(1752..2678)
                     /gene="rbsK"
                     /locus_tag="EYO68_09400"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIC08053.1"
                     /translation="MSKPISILGIFVADLTFRTDRMPLKGETFIGNSFKLGPGGKGSN
                     QAVATRRAGADTIFITKIGKDTFGEMAMKLYADEGINAEYVWEIPDMATGAASIVVNE
                     ETSDNVIIVVPGAADAMVPDDLDAAEAGIAKCAFFLASLEVPIPVMQRGLEVAIRNGV
                     PTILNPAPAAILPDDVYGLTDYFTPNETEAAMLAGIPVESVDDAQKAAKIFMERGVKT
                     AVITLGELGVYVRNSEISQHVPAFDMGENVIETTGAGDAFNGGFAHALAEGMPLIEAV
                     RFGSATAAISVTRLGTAPSMPFNNEILDLLNK"
     gene            3243..>4620
                     /locus_tag="EYO68_09405"
     CDS             3243..>4620
                     /locus_tag="EYO68_09405"
                     /inference="COORDINATES: protein motif:HMM:PF00589.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIC08054.1"
                     /translation="MPRQIRVSTEYSGVYFVKLANQDQSFFIRYKRNGKSVEEKAGRS
                     NQGWNAEKAYQLRTERMSGTSAAGNELQSNSDLLSQQDWTFSKIFSEYLRLRNKLKGR
                     ANDIYRFKNYLEKEFANITPSCVTQDDIERFKHNLQNRELKPATIRHVLELLRRLANF
                     AAKNNLCSGLSFKIQMPKVENYKTEELTNAQLQKLMQVLEEESDIQVSNLVRLALYTG
                     MRRGELFNLNWGDIDFYNKTITVKSDKKGDQPTIPLNEMAEKVLVEHAHTENGSKFVF
                     PGRGGKKRTECKRPLLRIREKAGLPDDFRILQGLRHVYASMLVSSGKVDLETLQSLLT
                     QKSPLMTQRYAHLLDESRTNSENIIADGEHNLSNATEEENYVSETVNAELLAEEVQET
                     DCPVEDVDTEHSEEEPLKDFIRETTYAEPLEKEIPETDIPEEQELTEFVQVAEQEVGL
                     HENVFSKSH"
BASE COUNT         1346 a          964 c          946 g         1364 t
ORIGIN      
        1 tctggcgaga gcatataaat agcgggcacg ctcatgtccg gaaagggctt tccacattgg
       61 aaatgcacgc tttgccgcac tcacggcaga atctacatcg ctttttcctg cttcagaaat
      121 cgaggccagt tttttgccat ttgccggatt gtctgtgctg aaaactttgc ccgactttgc
      181 tttgcaccat tttccattga taaaaagccc aaatttcgat ttatgttctt tgagccactc
      241 aagtgctaaa tcaggacttt caggtgcagg actgtattcc atagtttcgt aaatgtcttt
      301 gattttcatt aaagttttca gttttactcg ttacgtggtt ttgccgcgga atgcgtggat
      361 taagctgtgg gctgtctatg tgccgcagaa taacgtccgg taacgaaatg ctcaagctgc
      421 cgttcaatat ctgtcagtaa accgctggca ccaaaacgaa atagatccgg ttccagccat
      481 ttattcccaa gttcttcctt cattaaaatt agccactcca atgattgttt ggcattgcgg
      541 ataccccccg caggcttgaa tccaacgtat gatccggtga tttccagata ttcgcgaatc
      601 atacgaatca tcaccaaact cacaggaagt gtagcattga tactttcttt cccagtggag
      661 gtcttgatga agtcagctcc agccatcatg caaactaaac ttgcttttgc tacattacgg
      721 aaggttccga gttcaccggt tcccaaaatg gtcttcaaat gtgattcccc gcatgccttg
      781 cggaatgcag aaatttcatt gtaaagagct tgccaatctc ctgcaaacac atgtgatcgg
      841 gtaattacaa tgtcaatttc tttagcacca gcagctacag aatctttgat ttcttcaatg
      901 cgtcggggca tggaattaag tccatgtgga aatcctgtcg aaactgcggc aaccggaata
      961 ccggaatttt tcagggcatt tactgcagtt tccacataag catgaaaaac acagactgct
     1021 cctacacgcg gtttaagtcc gtttagacat aaattttcaa tcagttccgg acgcagcggt
     1081 tgacgcgctt tggcgcaaag acggtttaca cgatcaggtg tgtcatcact ggaaagagtg
     1141 gtgaggtcca tgcagcgtaa acttttcagc agccacgcgg cctgccattc ttttttgatg
     1201 ctgcgacgtt tcgtcaaggt tgcacaacgg cgttcgattg cacttcggtt catctgaata
     1261 ctgtccagcc agtctgaatt aaacgctata ccgggatttc tcgtgtgttt gtagtcggtc
     1321 aaaataatcc ttaaatttat tttttaaact cttcaatggt taagcctgca tccttcacaa
     1381 ttccagccat agtaaaggcg ttgataggat ttgcccgcgg aatgatcaaa attcgttctc
     1441 catttgtcat cgttacatgt tttcattgtc tcactacacg aaaaccctgt tttttcaacg
     1501 gctttaatcg ctcttttgtg agatactccc gaaagttttg gcatctctcg caacttctac
     1561 gtaacgtgtt tcactttctt gagtaagttc atcaactact gataaatagt ccacaatagc
     1621 atcctgaatg ttttccaaat cttcttcttc tgttgcaccc tgtgaccaac atcctggtaa
     1681 tccaggacat gaaacagcaa caccttcctc tgattttgta agtttgactt gataacgcat
     1741 aatttctcct tttatttatt gagtaagtcc aggatttcat tgttaaaagg catagacgga
     1801 gcagttccaa ggcgtgtcac ggaaatggca gcagttgcag aaccaaagcg tactgcttct
     1861 atcagtggca ttccttctgc cagggcatgg gcaaagccgc cgttaaaggc atctcctgct
     1921 ccagtcgttt caattacatt ttctcccatg tcaaaagcag gtacgtgctg actgatttca
     1981 gaattacgta cataaactcc aagttcaccc agtgtgatta ctgcggtttt aactcctcgt
     2041 tccataaata tttttgcagc tttttgagca tcatctactg attcaacagg tattccggcc
     2101 agcatggctg cttcagtttc gtttggagta aaataatcag tgagtccata gacgtcatcc
     2161 gggagtattg ctgctggtgc aggatttaag attgtgggca ctccgtttct tattgctact
     2221 tccaaaccgc gctgcatgac aggaatcggt acttcaagcg aggccaggaa aaaagcacac
     2281 tttgcaattc cagcctcagc ggcatccaaa tcatccggaa ccatggcatc agcggctccg
     2341 ggaacgacta taatcacatt atcactggtt tcttcattga ccacaattga agcggcgcct
     2401 gtcgccatgt ctggaatttc ccagacatat tctgcattga taccttcatc ggcatagagt
     2461 ttcattgcca tttcaccaaa tgtatctttt ccaatcttgg tgatgaagat tgtatcagca
     2521 ccggctctac gagttgctac agcctgattt gaaccctttc cgcccggacc tagtttgaat
     2581 gaatttccga taaaggtttc cccttttaga ggcatacggt cagtacgaaa cgtcaggtct
     2641 gccacaaaaa ttcccagaat tgaaattggt ttactcattt gtttttccct ccagctgatt
     2701 aaatatttgt ttgtgaaatt caaaagtcgg taagacacga taaactcaac tggcgtaaat
     2761 ataaagacag aaaaataaat cagatctttt cagttgaaat atggcataaa acaaaaataa
     2821 tttagtaatt taattatttt ttataacaca ttgataatac ttaatataat tcaataataa
     2881 tttttagact gcggggttaa ataattgcgg atatgtttga cacgaattat gcatagttat
     2941 ttaaaaataa tctttaattt tagtaatttg tttaagaaac acagtttaaa tgagttcttt
     3001 taatatttat aatttttctg ttttcaacct ttaaacgttg ggggataaat ttttgctaac
     3061 tgcaattatt actcgttagt catgagttat cgtaagatac tgatattgtg tactgctgtt
     3121 tgtcatttca acctgagtga aaaatttgct aaagtactta tatcgtacaa tattactctc
     3181 atatattttg gatgacacga caaaactcgt gactgtaagg atatttataa tcggagacta
     3241 ttatgccaag acaaataagg gtcagtacag agtatagtgg agtgtatttt gttaagcttg
     3301 caaatcagga tcaatcattt tttatccgtt acaaacgtaa tggaaaaagt gttgaagaaa
     3361 aggccggacg cagcaatcag ggatggaatg cggaaaaagc ctaccaattg aggactgaaa
     3421 ggatgtcagg aactagtgct gcaggaaacg aactgcagtc aaattcagat ttgctttcgc
     3481 aacaggactg gacgttttct aaaatatttt ctgagtattt gcgtttacga aacaagctca
     3541 aaggccgtgc aaatgacatt taccgcttca agaactacct tgaaaaggaa tttgcgaata
     3601 ttaccccttc atgtgtcact caggacgata tagagagatt taagcacaat ctccaaaaca
     3661 gagaactaaa accggccact atccgtcacg tccttgaact gctcaggcgt cttgcaaact
     3721 ttgcggccaa aaataatttg tgctccggac tgagttttaa gattcaaatg cccaaggttg
     3781 aaaattataa aaccgaggaa ttgactaatg ctcaattgca gaaattgatg caggtattgg
     3841 aagaagaatc ggatattcaa gtcagtaatc tggtgcgtct tgcgctctat accggaatga
     3901 ggcgtggtga actgtttaat ctaaactggg gtgatattga cttttacaac aaaaccatca
     3961 ccgtcaaatc cgacaaaaaa ggagaccaac cgacaattcc tttgaatgag atggctgaaa
     4021 aggtactggt tgaacacgct cacactgaaa acggcagcaa gtttgtcttt cccggacgtg
     4081 gtggtaaaaa acgcactgag tgcaaacgac cattgctgag gatcagggaa aaagctggac
     4141 tgccggatga ttttcgtatt ttacagggtc tgagacatgt atatgcctcc atgctggtat
     4201 ccagtggaaa ggtggatctt gaaaccctgc agtctctgtt aacgcagaaa tcgccactaa
     4261 tgacacagcg ttatgctcat ctgcttgatg aatcacggac aaattcggaa aatataattg
     4321 ctgatggaga gcataactta tcaaatgcaa ctgaagaaga aaattatgtt agtgaaacag
     4381 taaatgcaga acttctcgca gaagaagttc aggaaactga ttgtccggta gaagacgtgg
     4441 atacagaaca ttcagaagag gaaccgttaa aagattttat ccgtgagaca acctatgcag
     4501 aacctcttga aaaagaaatt cctgaaactg atattcctga agaacaagag ctaactgagt
     4561 ttgtgcaagt agcagagcaa gaggttggtt tacacgagaa tgtgttttca aagtcacatg
//
LOCUS       DTVD01000252            4616 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6528_length_4616_cov_253.434115, whole genome
            shotgun sequence.
ACCESSION   DTVD01000252 DTVD01000000
VERSION     DTVD01000252.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_4616_cov_253.434115"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            101..832
                     /locus_tag="EYO68_09410"
     CDS             101..832
                     /locus_tag="EYO68_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08055.1"
                     /translation="MGIAKNFRGQYYKKIITLCGFLLLTFSAGAETIYELDFSTASGN
                     VQNWFEKIKWEFREDITDMNLRFEDGKLVIEPTRDELGVMMLQFDEKDYLHGETKIRI
                     EWGVDQYPLGADWSGPKEKTRNTREAISFMVFFGETKLDSGLFLAPNLPYFISFFLGK
                     DEKPDQVYFGNYWQEGGRYLCIPCDGSIGKTYITEIDLSAKFFELFGKKQLPVTALGI
                     EVDVQTTEKVNGRHSKAFIKRVELF"
     gene            1023..1442
                     /locus_tag="EYO68_09415"
     CDS             1023..1442
                     /locus_tag="EYO68_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIC08056.1"
                     /translation="MKIKIKILSITLVVFLFNPQLFADIYEVNNNKIIILMESGVPLI
                     DIRTKREWYETGVIKKSNLLTFFDKDGNYNVKEWMSKLKKIASNKDPVIIICRSGRRS
                     RIVANFLDQKEHYTTVFHATDGIISWIDFKNKTVVPD"
     gene            complement(1500..2354)
                     /gene="argB"
                     /locus_tag="EYO68_09420"
     CDS             complement(1500..2354)
                     /gene="argB"
                     /locus_tag="EYO68_09420"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIC08057.1"
                     /translation="MAVFTEEKKKLLIEALSYIKSFNGKIVVIKYGGAAMIEEHLKHD
                     FAQDIVLLQSLGMLPVIVHGGGPEVSKAMERLGQKVSFIDGLRVTSSENMKVAEMVLS
                     GTINKEIITHLNTFGGKAVGVSGKDGHLIEAIKKPQKSGIDLGFVGEIKNVNPELLLV
                     LLKQGFLPVVSPIGLGKDGITYNINADTTASRIAVSLAAHKIIFMTDVDGVLKKGKLI
                     AQLTASEAEKLIQQKVISGGMAPKIEAGLFCVNNGVESAQIINGTDSHSVIAELFTDK
                     GIGTKIVL"
     gene            complement(2451..4331)
                     /locus_tag="EYO68_09425"
     CDS             complement(2451..4331)
                     /locus_tag="EYO68_09425"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01464.18,HMM:PF01476.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIC08058.1"
                     /translation="MKFKILQNNISCFLVILLIFTFVKQLEAASPSYQPAPLRNQSLL
                     FETSGKAIRKIKLDVSSIKKPVVKKITNSKTPYRNKFWKVEGKRYDIPIRVNSKVKSI
                     LRKFASKNRKQLIVGIQRSGKFMPMITRMLQKEGLPLDLAYMVPVESNFNVRARSKKS
                     AVGLWQFIASTGRIYGLKINRWIDERRDPVLATKAAITYLKHLYGIFGDWELAMAAYN
                     SGEGRVKRAINRAKKKGRKHNFWSLRLPRETRGYVPSIMAMAVIYKNPKRYGFGHVRP
                     LSPMDETKASLTVAFSLEEVAKRSKMSFSALRDKNPALFLGVPPMTQTSYSFYVPQNN
                     RQELMASLKQNPNPSKKWGHSYNALLGSSNRVTRVLEKFGAPIYFRVKTGDNLWDLAR
                     KHKTSIRRLARWNRLNSKSVLRVNRRLKIYVPTWRVFREVARNSSYSSTRLIALRIRV
                     PKGGALSVIAEKYKTSVRKLMRWNKLSSPHAIRAGQRLVVGYRKASNSNLPREASVIR
                     VPRNTTLSHLAIRYKTSARKLMTWNGLTNSRQLRTGMRLYVKAPEKIKRKQQKRIVAK
                     KNSRRLIKVRHKIIRVRNGDTLWGIARIYRTTVKALVALNELKSESHLRLNQKLIVPY
                     PS"
BASE COUNT         1390 a          885 c          888 g         1453 t
ORIGIN      
        1 aattgggagc aatacgtact gcaattgctc ctccggctgc tttttaatca agaaactctc
       61 agttattcag caaagtattt atcggcttaa aaaggaatta atggggatag caaaaaactt
      121 ccgtggacaa tattataaaa aaataattac cctgtgcgga tttttattgc tcacattttc
      181 tgccggggca gaaacgattt acgaattgga tttttcaact gcatccggca atgttcaaaa
      241 ctggtttgaa aaaatcaaat gggagttccg ggaagatata accgatatga acctccgttt
      301 tgaagacgga aagcttgtga tagagccaac tcgtgatgag ttaggtgtga tgatgctgca
      361 gtttgacgaa aaggactatt tgcatgggga aacaaaaata cgaatcgaat ggggagtaga
      421 tcaatatccg cttggggcag actggagcgg tcccaaagaa aagactagaa acacacgtga
      481 agcaatttcc tttatggtct tttttggaga aactaaactg gacagcggac tttttttagc
      541 tcctaaccta ccttatttca tcagtttttt tcttgggaaa gatgaaaaac cggatcaagt
      601 ttatttcggc aactactggc aagagggagg tcgttatctc tgcataccct gtgacgggag
      661 tattggaaaa acatatatca cagaaattga tctctcagca aaattctttg aattatttgg
      721 taagaaacag ttgcctgtaa cggctcttgg gatagaggtt gatgtgcaaa ctactgagaa
      781 agtaaatgga cgtcactcaa aggcgttcat taaacgggtt gaattattct aataaaaaca
      841 caatgaattt ttttaaaaaa actcttgatc caatgattgc actgccactg atagcggtcc
      901 tggaaatttt tcttttcctg ctggttggcg catggacaga ccaaaaggtt tttactatgg
      961 aaacgagtca gaaataaaac aatctgagca catcaaacta aatgtataaa tttgttaaat
     1021 aaatgaaaat aaaaataaaa attctctcta taactcttgt tgtcttttta ttcaatccac
     1081 agctgtttgc tgatatatat gaggttaata ataataaaat aataattcta atggaaagtg
     1141 gggttccatt aatagacata cgaactaaaa gggagtggta cgaaacggga gtgattaaaa
     1201 aaagtaatct tctaacattt tttgataaag atggaaatta caatgttaaa gaatggatgt
     1261 caaaactgaa gaaaatagca agtaataaag accctgtaat tataatttgt agaagtggca
     1321 gaagaagtag gattgttgct aactttctag accaaaaaga acattataca actgtttttc
     1381 atgcgacaga tgggattatt tcttggatag attttaaaaa taaaaccgtt gtacctgatt
     1441 aaagtctttc tgtgaaattg ttttactttg cagagcaaca ataattcttt ttaaaattgt
     1501 taaaggacaa tctttgtacc aatacctttg tcggtaaata attctgcaat gacagagtga
     1561 gaatctgtac cgttgattat ttgagcagat tccaccccat tatttacgca gaaaagcccg
     1621 gcttcaattt ttggtgccat tccgccgctg atcacctttt gctggatcaa tttttcggct
     1681 tcgcttgcgg tgagttgagc aattaacttt ccttttttaa ggacaccgtc tacgtcagtc
     1741 atgaagatta ttttgtgtgc cgccaaagaa acagctatgc gggatgcagt tgtatctgca
     1801 tttatattgt atgtaatacc atcttttcca agaccaatcg gtgagacaac gggtaaaaaa
     1861 ccctgcttta gcaaaaccag cagtagttcc ggattaacgt ttttaatttc accaacaaag
     1921 cccaaatcaa tgccgctttt ttgaggcttt tttatagcct caatcaagtg cccatcttta
     1981 ccgctgactc ctacagcctt accgccaaaa gtattcaggt gtgtaattat ttctttgttt
     2041 atagttccag agagtaccat ttctgcaact ttcatatttt ccgaacttgt aactctcaga
     2101 ccatcaataa aggagacttt ctggcctaat cgttccattg cctttgaaac ttccggacct
     2161 cctccatgaa caattacagg cagcattccc aatgactgga gcaaaacaat atcctgggca
     2221 aagtcatgtt tgagatgctc ctcaatcatg gcagcacctc catatttgat gaccactatt
     2281 ttaccattga agctctttat gtaagaaaga gcttcgataa gtaatttttt cttttcctcg
     2341 gtaaagactg ccattaaaaa gccgttttga atttatattt ctgaaataaa gataatgttg
     2401 gattttgatt aaaatgcaaa aatgtctgca aaaacaaggg gacgaatgat ttatgagggg
     2461 taaggaacaa tcaatttttg attgagtctc aggtggcttt cagacttcag ctcgttaagt
     2521 gcaaccaacg ccttaactgt ggttcggtaa attctggcaa ttccccacag agtgtcgcca
     2581 ttacgtacac gaataatctt gtgccggact ttaattaatc ttcttgaatt cttttttgca
     2641 acaattcttt tttgctgttt tcgtttaatt ttttcaggtg cttttacata aagcctcatt
     2701 cccgtccgaa gttgcctgga attagttaaa ccgttccagg tcatcagttt tctcgcagat
     2761 gttttgtagc gaatagctaa gtgagacagt gtagtatttc gcggaacacg aatcacactt
     2821 gcttcgcggg gtagattact gtttgaagct ttacggtatc caaccactaa cctttgcccc
     2881 gcgcggattg catgagggct gctcaattta ttccaacgca tcaatttcct gacactagtc
     2941 ttatatttct cagcaattac agacagagca cctccctttg ggacccgaat tctcagggca
     3001 attaatcttg tactggaata agaagaattt ctggcaactt ccctgaaaac ccgccaggtg
     3061 ggaacataaa tctttagtct gcgattgacc cgcaaaacag atttcgaatt aagcctgttc
     3121 caccttgcta atcttctaat agatgtttta tgtttacgag ccaagtccca taaattgtct
     3181 cctgtcttaa cgcgaaaata aattggtgct ccaaattttt caagtactct tgtcacccga
     3241 ttagaactgc ccagaagggc attatatgaa tgtccccatt tttttgaagg atttgggttc
     3301 tgcttcagag aagccattaa ctcctgtcga ttgttttgtg gtacatagaa cgaataactc
     3361 gtctgagtca taggtggtac gcccaggaac aaagcagggt ttttatctcg aagtgcactg
     3421 aatgacattt ttgagcgctt tgctacctct tccagagaaa aagctacagt caaactggct
     3481 tttgtttcat ccattggtga aagtgggcgt acatgtccaa aaccatagcg cttcggattt
     3541 ttatatatga ctgccattgc cataatagac gggacataac ccctggtttc tcgaggcaga
     3601 cgcagtgacc aaaaattgtg tttacgtcct tttttcttcg ccctgtttat agctcttttt
     3661 acacgtcctt ctcctgaatt ataagcagcc atagcaagtt cccaatcacc aaaaataccg
     3721 taaaggtgct ttaggtaagt gattgctgct ttagtcgcga gaacaggatc cctcctttca
     3781 tctatccaac gattaatttt taagccgtaa atccgtccag ttgaagcaat aaattgccat
     3841 aatcctacag cacttttttt agaccgagcg cgaacattga aattactttc aaccggaacc
     3901 atgtaagcca ggtccagcgg cagtccttct ttctgcagca tcctggttat catgggcata
     3961 aatttgccag aacgctgaat acccactatt aattgcttac gattctttga tgcaaacttc
     4021 cgtaggatac ttttgacttt tgaattaacc ctgattggta tgtcataacg cttgccttca
     4081 actttccaaa atttattgcg gtaaggtgtt ttggagttgg ttattttctt tacaacaggc
     4141 ttttttattg aggaaacatc caacttaatt ttccggatag ctttaccgct tgtttcaaat
     4201 aacagtgact gattccgaag cggtgctggc tggtaagaag gggaggctgc ctcaagctgc
     4261 ttgacaaagg taaaaatgag aaggattacc agaaaacagc ttatattatt ttgtaaaatt
     4321 ttaaatttca tgaaagttta gtttttacag gtaatctttc ttggaattac agaaaaggtg
     4381 aactttttta attatcaaat gtgcatgata tcttatcaag ctctttctgt acgtcatcct
     4441 aagacagagt gcaaagatca tgcataaaaa cttaattatt tttactattc ttctcatatt
     4501 aaaaatagtt tgactatctt cgttttacaa atagaatgaa tatgtattat ccaacaaagc
     4561 gagaatagct cagctggtag agcgcaacct tgccaaggtt gaggtcgcgg gttcga
//
LOCUS       DTVD01000253            4595 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6580_length_4595_cov_401.631718, whole genome
            shotgun sequence.
ACCESSION   DTVD01000253 DTVD01000000
VERSION     DTVD01000253.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6580_length_4595_cov_401.631718"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1192)
                     /locus_tag="EYO68_09430"
     CDS             complement(<1..>1192)
                     /locus_tag="EYO68_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HIC08059.1"
                     /translation="FQKQPFHIPLALGLLAADGDTLPLKLTGCTAVKNQDTIILELRK
                     QSEIFVFENIPAAPVPSLLRGFSAPVKLLFDYSIADLAFLLANDTDEFNRWEAGQQLM
                     IRISLEQIQRFQNHEPFNLPSELENAFRSLLNQTQEGDSALLALALSFPNEPYLGEFM
                     DVIDVDAIHETRTFLRKELAQKLQPEFENTYLEFKEEGAFKIDQQSMGRRSLKNVCLS
                     YLSELGSLDIRKLTQTQFRKNENMTDVAGALGVLTHLDCPERETAFSEFENRWRKNTV
                     VMDKWFALQAISCLPKTLDHVRKLTSHSAYEKNNPNKIRALISTFSRFNQLRFHAADG
                     SGYEFIAEQVLRLDPLNPQIAARLVSVFNNWKKFTETHKTKMNYQLQKIVKTPKLSGD
                     VFEIV"
     assembly_gap    1193..1292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1293..2728)
                     /gene="pepN"
                     /locus_tag="EYO68_09435"
     CDS             complement(<1293..2728)
                     /gene="pepN"
                     /locus_tag="EYO68_09435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIC08060.1"
                     /translation="MKTQTPQTIYLKDYRPPQFLIDTVDLHIDLAEEWTTVKAELNFR
                     RNPASAENSKTLVLNGHKMELMAVILDNVELTQDQYQVDESQLTINDVPEKFVLSTEV
                     RIQPQNNTELEGLYKSSKLFCTQCESEGFRKITYFLDRPDVMSLYSTTISADPQLYPV
                     MLSNGNLLDSGKLTDGRHWVKWEDPFPKPAYLFALVAGDLLHIEDSFRTSSGREVKLQ
                     IYVEAENIEFCDHAMRSLKESMRWDEERFGREYDLDLFMIVAVNDFNMGAMENKGLNI
                     FNSKLILASQETATDTDFYNIQGVVGHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDQ
                     EFSSDLNSRAVQRIADADRLRVHQFPEDAGPMSHPIRPDSYMEINNFYTMTVYEKGAE
                     VVRMIYTLLGRENFRKGTDLYFDRHDGQAVTCDNFVAAMEDANGVDLQQFRRWYGQSG
                     TPELHIRGSHDPEAKTYSLTVRQSCPDTA"
     gene            2905..3813
                     /locus_tag="EYO68_09440"
     CDS             2905..3813
                     /locus_tag="EYO68_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIC08061.1"
                     /translation="MESGNVKNHHEHGHHHSGDVEALGGAFLLNFIFTLIEIAGGLWT
                     NSVAILADALHDAGDSLALLLAWYLQKASGKERDQRFSYGYGRLSLLAALINGVVLLA
                     GSIVVIVHVIPRLFEPQIVDATGMFWLALLGIAFNGFAFWRNRKSQSLNAKMVNWHLM
                     EDVLGWTVVLIGSIAMHFGDFPWLDPLMALGVTLFILWNVFRSLRHVARIFLQSNPED
                     LDLDALESELRLMNNVEDVHDVHAWSLDGKYHVLSLHVVISQIPDQETLVQLKNQIRE
                     QTRKMGIDHATIEIELPSEACVLENC"
     gene            complement(3855..4547)
                     /locus_tag="EYO68_09445"
     CDS             complement(3855..4547)
                     /locus_tag="EYO68_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIC08062.1"
                     /translation="MKDSSNSISTALIPTIPLDYQEYPIEEVQRRSLEYYENMKRRRT
                     VRDFSRRKVPLDIIENCLRTADTAPNGANQHPWHFVVVSDPEIKLQIREAAEKEEQDF
                     YKSRASREWLEALAPLGTDENKPFLETAPYLIAIFAKVYGLDEKGKRVKHYYVNESVG
                     IATGLLISAIHHSGLASLTHTPSPMRFLNQILERPERERPFLLLVVGYPAADARVPDI
                     QRKSLPEISTYF"
BASE COUNT         1184 a          954 c         1009 g         1348 t
ORIGIN      
        1 acaatttcaa aaacgtctcc ggaaagtttt ggtgttttaa caattttctg aagttgatag
       61 ttcatttttg ttttatgtgt ttcagtaaat tttttccagt tattgaaaac acttaccagc
      121 cttgctgcaa tttgtggatt cagtgggtcg agcctcaaaa cctgttctgc aataaactca
      181 taaccggaac cgtccgcggc atggaatctc aactgattaa agcgtgaaaa cgtgctgatc
      241 aacgcccgga ttttattagg attatttttt tcatatgcag agtggctcgt caattttcgc
      301 acatgatcca gtgtttttgg cagacatgaa attgcctgca aagcaaacca cttgtccatc
      361 accaccgtgt tttttcgcca cctgttttca aactcgctaa aagccgtttc acgttccgga
      421 caatccagat gagtcagcac tccaagcgcg cctgcaacgt cagtcatatt ttcgttttta
      481 cggaattgag tttgtgtcaa tttgcggata tccaaacttc ccaattctga taaataactg
      541 agacagacat tttttaaact acgccgcccc atcgattgtt gatcgatttt gaatgcgcct
      601 tcttccttaa attccagata agtattttca aattcaggct gtagtttttg tgccagttct
      661 ttgcgtagaa atgtacgtgt ctcatgaatc gcgtccacat caattacgtc catgaactca
      721 cccaaatatg gttcatttgg aaaacttaat gccagcgcca aaagtgcgga atcgccctcc
      781 tgagtttggt tcaggaggct ccggaaagca ttttccaatt ctgatggtag gttaaatggc
      841 tcatgatttt gaaaacgctg tatttgttcc aatgaaattc ggatcatcag ttgctgacct
      901 gcttcccagc ggttaaattc gtcagtgtca ttggccagta aaaaggctag gtcagcaatg
      961 ctgtaatcaa aaagcagctt taccggtgca gaaaatcccc tcaaaagtga tggaactggc
     1021 gctgcgggaa tgttctcaaa gacaaaaatt tcactttgct tgcggagttc caggatgatt
     1081 gtgtcttgat tttttacagc agtacagcct gttaatttta acggcagagt gtcaccgtct
     1141 gctgcaagca aacccaacgc taaaggaatg tgaaacggtt gtttttggaa agnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcagtgtc agggcaggat tgtctaactg
     1321 tcagggaata tgtttttgcc tccggatcat gtgagccgcg gatgtggagt tccggtgtgc
     1381 ctgactgacc gtaccagcgc ctgaattgct gtaaatcaac tccgtttgca tcttccattg
     1441 ctgcaacaaa attgtcacag gtcacggctt gtccatcgtg tcgatcaaaa taaagatccg
     1501 tgcctttgcg aaaattttct cggcccagca gagtatagat catacgtact acctcggcac
     1561 ctttttcata aactgtcatg gtgtagaagt tattgatttc catgtaagaa tcaggacgta
     1621 tcggatgact catcggtccg gcgtcttcag gaaactgatg cacacgcagg cggtccgcgt
     1681 ctgcgatacg ctgcactgct ctggaattca ggtctgagga aaattcctga tcacggaaaa
     1741 cggtgaaacc ttccttgagg ctcagttgaa accagtcacg gcaggtgaca cgatttccag
     1801 accagttgtg aaaatattcg tgtccaacca caccctggat attgtagaaa tctgtgtcag
     1861 tcgcagtttc ctgagaagca aggatcagct tggaattaaa aatattgagc cctttgtttt
     1921 ccatggctcc catattgaaa tcattgactg ctacaatcat gaacaaatca aggtcatact
     1981 cccgcccgaa acgttcttca tcccagcgca tcgactcttt gagcgagcgc atggcgtgat
     2041 cgcaaaattc aatattttct gcttcaacgt atatttgcaa tttcacttca cgtccggaag
     2101 atgtccggaa tgagtcctca atgtgcagta aatctccggc gaccagcgcg aaaagataag
     2161 ccggttttgg gaaaggatct tcccatttta cccagtgtcg gccgtcagtt agttttccgc
     2221 tgtcgagcag atttccattt gaaagcatca ccggatagag ctgcggatcg gcggaaatgg
     2281 tcgtgctgta aagtgacatg acatccggac ggtccaggaa atatgttatt ttacggaaac
     2341 cttctgattc gcattgagtg cagaacagtt tgctggattt gtaaagaccc tcaagctcgg
     2401 tgttgttttg cggttgtatc cggacctccg ttgacagtac aaatttttcc ggaacatcat
     2461 taattgtaag ttgagattca tcaacttgat actgatcctg cgtcagttcc acattatcca
     2521 gaatgacagc cataagttcc atcttatgtc cattgagtac cagtgtttta ctgttttcag
     2581 cggaagcagg atttcgcctg aaattcaatt ctgcttttac tgtcgtccat tcttcagcta
     2641 aatcaatgtg caaatccacc gtatcgatca aaaactgtgg aggcctgtaa tctttgagat
     2701 aaattgtttg aggggtttgg gttttcatgc tattcttcca ccagctggag ttgagttttt
     2761 attgccagaa tctgaagaat aatgaacctt taagatagaa aaaatcaatg gagaaaattc
     2821 gtgtttttca gacatatttt ttataccagc tgttaatagt tatacttttt aagtagacga
     2881 taataataaa aaaactacag aaaaatggaa agcggaaacg tcaaaaatca ccatgaacat
     2941 ggtcatcatc attcaggaga tgttgaagca ctcggcggcg cgtttctgct aaattttatt
     3001 ttcacgctga ttgaaattgc gggaggatta tggacaaaca gcgtcgctat tttagcggat
     3061 gctttgcacg atgcaggtga ttcattggcg ttgttactcg cgtggtattt acagaaagcg
     3121 tcggggaagg aaagagatca gcggttttcc tatggttatg gacgtttatc acttttggcc
     3181 gcgctgatca atggagtggt ccttctggca ggttcgattg tggttattgt acatgtcatc
     3241 ccccgcctgt ttgaaccaca gattgttgac gcaacaggta tgttttggct ggcattgctg
     3301 ggaattgcgt ttaatggttt tgctttttgg cgcaatcgaa aaagtcagtc cctgaatgca
     3361 aaaatggtca actggcattt aatggaagat gtgctgggct ggacggttgt gctgatcggc
     3421 agtattgcca tgcactttgg tgattttccc tggctggatc cattgatggc actgggagtg
     3481 acgctcttta ttttatggaa tgttttccga agtttgagac atgttgccag aatatttttg
     3541 caatccaatc cggaagacct tgacctcgac gcacttgaaa gtgaactgcg tttaatgaat
     3601 aacgtagaag atgtacacga cgtacacgcc tggtcgctgg atggtaaata ccatgtgctc
     3661 agcttgcatg tggtgattag tcaaattcct gatcaggaga ctctggttca actcaaaaac
     3721 caaattcgcg agcagacacg gaaaatggga attgatcatg ccactattga aattgaactt
     3781 ccttcagaag catgtgtcct ggaaaattgt tgatgattca tctctgcatg ttgctttttc
     3841 ctgctgatct tccctcaaaa atatgtagaa atttccggca gagattttct ctgaatatcc
     3901 ggaactctgg catctgctgc aggatatcct acgaccagta ataaaaaggg gcgttccctt
     3961 tccggacgct ccaggatttg gttcaggaaa cgcattgggc ttggagtatg ggtgagtgac
     4021 gccaatcctg aatggtgaat ggcgctgatc aataatcctg tggcaattcc cactgactca
     4081 tttacataat agtgtttgac acgtttccct ttttcgtcaa gaccatatac cttggcaaaa
     4141 atggcaatca aataaggagc agtttctaaa aagggtttgt tttcatccgt tcccagtggt
     4201 gcaagagctt cgagccattc tctgcttgcg cggcttttgt aaaaatcctg ctcctccttt
     4261 tccgcagcct cccggatttg aagcttaatt tccggatcag agaccacgac aaaatgccat
     4321 ggatgctgat ttgctccgtt cggggcagtg tctgcggtac gtaaacaatt ttcaataata
     4381 tcaagaggta ctttacgcct ggaaaaatcg cgaaccgttc gacgccgctt catattttcg
     4441 tagtactcca gcgaacgacg ctgaacttct tcaattggat attcctgata atccaggggt
     4501 atagtgggaa ttagagcggt agagattgaa ttactgctgt ctttcattac acatttattt
     4561 tgaggagaaa acgatgtgac tacatcatgc aattt
//
LOCUS       DTVD01000254           16447 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_663_length_16447_cov_325.522572, whole genome
            shotgun sequence.
ACCESSION   DTVD01000254 DTVD01000000
VERSION     DTVD01000254.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16447)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_16447_cov_325.522572"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="EYO68_09450"
     CDS             complement(<1..663)
                     /locus_tag="EYO68_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08063.1"
                     /translation="MRIIKHISFLLVGMAVILVLAFWGFKQNFPGKSIADAVRFRLTS
                     QTGIPFEIQDIELGWSKISTPEIVLRTPKWLAGIPDIRLLILENLEVPFFSIITSGKA
                     KVHGQVHEGAFRISTELLTQKILDLSIDSVQIERVPLFSLVPYTFVSGFLSLSAHIEN
                     FNALQQQKTKFPEGTIKGKLKNARIRISGGTALLDLQLPELDLSEVQFEVQLGRSIHI
                     KKI"
     gene            complement(670..1155)
                     /locus_tag="EYO68_09455"
     CDS             complement(670..1155)
                     /locus_tag="EYO68_09455"
                     /inference="COORDINATES: protein motif:HMM:PF04612.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08064.1"
                     /translation="MPNLSMREQLLISSMFLIIFVLAGMWGIQQLNRQEKMLLQQLGK
                     LEQQIKQVRFLSRELKDVQRSATASVMPQALSSFVEKIARSLEIQDNLQLNVLTNVQK
                     GTEGVKVGLDRLKLDHLMSMLYQLENHRPVLRTSHLSISISPGNRQVRASFQVHKQLG
                     G"
     gene            complement(1184..2467)
                     /locus_tag="EYO68_09460"
     CDS             complement(1184..2467)
                     /locus_tag="EYO68_09460"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIC08065.1"
                     /translation="MANTARLEVNEKSIDLPLVMGSESEIGIDISKLRSQTKAITLDP
                     GFVNTGSCESGITYLDGENGILRYRGYPIEQLAEKSNFLEVSYLLIYGELPTNQQLND
                     FEYNVKQHTLLHEDVRHIYQAFPKDAHPMAILSSIVCALSTFYPDFSATDSDAVDLAI
                     WRLMGKLPTIAAWSYKKNIGQPFVYPINEFTYTKNFLNMLFGVPTTNYEVPEEFVKAL
                     NVLFILHADHEQNCSTSTVRLVGSSEANLFASISTGIAALWGPLHGGANQAVLEMLKQ
                     IHNDGGDVQKFVKLAKDKGSGFRLMGFGHRVYKNFDPRATILKGYCDKILDLLGTPDP
                     LLDIAKQLEEAALTDEYFIERNLYPNVDFYSGIIYKALGFPTDMFTVLFSLGRLPGWI
                     AHWLEMRNGISKIGRPRQIYTGQTERNYVSLSQRN"
     gene            complement(2624..3025)
                     /locus_tag="EYO68_09465"
     CDS             complement(2624..3025)
                     /locus_tag="EYO68_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HIC08066.1"
                     /translation="MLLKLFLAFTIIPIFEIYLLIKIGTVFGALTSIALVIFTGFLGA
                     YLARIQGLQTLFRIQESLREGRMPSGELLDALLIGVAGLVLLTPGFLTDTIGFILLVP
                     SSRNAIKSWLRRQIEARYSSTRPEDVIIEQE"
     gene            complement(3066..4349)
                     /locus_tag="EYO68_09470"
     CDS             complement(3066..4349)
                     /locus_tag="EYO68_09470"
                     /inference="COORDINATES: protein motif:HMM:PF07705.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08067.1"
                     /translation="MFRILLLKLIFSGIIVLFLPTLLSANQSPDLKIIAIVLSKTCPL
                     PDGPDMTIDSPDSIIKSVNNPDCPKHGDTQLVQTAIANHAKIQIKVGVEIKVRVDNQE
                     LKKKGLRKVYTIQARDTTRVLHDIDLGDPGLYQISVRVWDAKFKRILINTTAGDERYF
                     FVASHQDVEVAKTQLASGVRVSGKNVISPLRFDPPDLRWESVQVLPKHALRGEKLKLR
                     LNLMNAGGDIVRDILAKVQYYNVKQPMRKSVIAMPSVHVMAPGEITTFDLEYILPDDQ
                     LLGKYQIIATVDPDNEIEELKEKNNAAKSNVIKLSDIKLLLPTDKFTFEENGLFLFQW
                     DSLAFSEFKIQVGVDEKFEDSGLYFDLPQGNRWIADKELVPLAGELPGMAMGLMRAKE
                     KNQLYWRVIGRQASGRQAVSGINRFSITPVSSDSS"
     gene            complement(4583..5455)
                     /locus_tag="EYO68_09475"
     CDS             complement(4583..5455)
                     /locus_tag="EYO68_09475"
                     /inference="COORDINATES: protein motif:HMM:PF02616.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIC08068.1"
                     /translation="MDGMETMEQIKKSVEIPAIFPNPENPLEIKIPLFEGPLDLLLHL
                     IRKKEVSIEEVRLSELTSSYLEYVEMMQTINLDLAGEFLEIATLLILIKSRYLLPKSP
                     EDIEEVEEEDPEEILRLRLLEYQHYKDAAFELSSLDILGRDVFQRPENVELVADNEET
                     APEYEDISVSALMEAFGRVMKRLPKITRHLIETENVRIEDRIDELISLFGKRKHCLTE
                     ELFEDNKPLSWFIITFMAILEMVRLHLITIVQVDQLGAIHCTVHDDFDQNISHWYHLQ
                     NTNGKEETVLLKAS"
     gene            complement(5476..8211)
                     /locus_tag="EYO68_09480"
     CDS             complement(5476..8211)
                     /locus_tag="EYO68_09480"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00263.19,HMM:PF03958.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08069.1"
                     /translation="MIFFLSILSEQYRKFHRLSGIIFLSLFLFQASFPVEIEAAKKKK
                     SSKVTINLPQTLILKEFIKIISEHTGTVFVYEEKNMRGQMSITAPRNFKVSAEDAFYF
                     FEKILASQGLAMVRKKGSKVVEILTAADARFSRLPISKDGGKVNAKGGNYVMRLIPIR
                     HADLKGIQSTLQPIFSKTGALLVYEPMNMLIVIDVESNVSRIVELIDMLDISAPEGVE
                     QIVTLLQIVHNDVTEIHKTVSELFSNIVLNGKPPKFKLLIEARLNSLFIVANRETTAE
                     IIELIEQVDVPVEGATTTIHELQYSDAAKLIPLITTVFPKTSSIKILPFAPFNALIII
                     ANPVTTQQIIDLVDQLDIPRGNRQIKLQTLQYAYATTLAPLLSKIFADKIVAGKGEGK
                     TATGSAVKIIAEPRLNALIIIADRLATDRVLQLIAKLDTVQGTSGETQLKLVPLENTS
                     AKRMAVLLSKIFSDSVVAGKGEGKTAKASPVKIIEEERLNSLIIIAGRIEIEQILSLI
                     KKLDVFQESGKIKSNFKLYHLKHAVAKDMAVLLKEVTGRITEVARKDEKPKPESQNQS
                     KTVGSGGQTDISISADEATNSLLIFAPSDAFTTLDQIIIELDVPRMQVYVEALVMEVS
                     LSKSLDLGINWKASGVTSGGRVITGGFPGAGAFTGDASTGGTAAIAANSSTIGVLNGN
                     TISIGGKEFFSFGAFVKAVQNNNDVNVLANPQLMIINNPQEGEIPNINVVSVVPVSPK
                     TVTSASNITTKEYQYKEVGIKLEIKPQISGENSIRLDISQESSDVVNPGAEAITIFKR
                     TIKTSVIAEDGEIIVLGGLIKEKVARNQNKIPGFGDLPLIGWLFRSKLDQFEKINLLI
                     FIRPTIIRTKKDLEKVNQRASARFKKAKELNKVSDQVLEDMGIPELSDNLDKVTE"
     gene            complement(8239..8928)
                     /gene="ispD"
                     /locus_tag="EYO68_09485"
     CDS             complement(8239..8928)
                     /gene="ispD"
                     /locus_tag="EYO68_09485"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:PF01128.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIC08070.1"
                     /translation="MAEQDTVAVIVAAGQGTRMGAKHNKLLLPLGTRTILEIGLETFI
                     KHPRIRKIYLTVSSQDRQIFENMIPEEIVLVEGGSRRQDSVHNALLEIMQDEQVPELV
                     LVHDGARPFCSEKLIDRIIDAAHEHNAAIPVLPLEDTIRRITEEKTEVLDRRALFATQ
                     TPQGFRTKLILDASKQAIENKWLVTDDASLVENTGDQVSTVEGEPQNIKITTPADLER
                     ANWILKSMSNS"
     gene            9180..10070
                     /locus_tag="EYO68_09490"
     CDS             9180..10070
                     /locus_tag="EYO68_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC08071.1"
                     /translation="MSEKTAFIGLGVMGYPMAGFLAKAGNEVTVYNRTTVKAEKWVEE
                     YGGKLALTPAEASKDAAFVFACVGNDNDLRSVTIGTDGAFTGMSSGTVFVDHTTASAK
                     IARDLADAAKLKECAFLDAPVSGGQAGAENGVLTVMVGGDQDSFDQAEPLIQNYAKKV
                     KLLGPVGYGQLTKMVNQICIAGLVQGLAEGIHFAKAVGLDVDTVIETISKGAAQSWQM
                     ENRYKTMNEGKFDYGFAVDWMRKDLGIALEEARANKAHLPVTALVDQFYSEVQALGGS
                     RWDTSSLIARLEAMQQMNNK"
     gene            10085..11533
                     /locus_tag="EYO68_09495"
     CDS             10085..11533
                     /locus_tag="EYO68_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC08072.1"
                     /translation="MHKFKGKILIEGKWQTPDSGETLPVIDPSDGCEFGRIARGSAVD
                     IDLAVKSAQNAMQGEWGKTIPVERGRLLQKLSSLILENEDELTEMEARDVGKPLRQAR
                     VDVKACARYFEFYGGAADKVHGETIPYLEGYTVLTLREPHGVTGHIIPWNYPLQILGR
                     TIGGALTMGNACVLKPGEEASQTALMVGELAIQAGFPPGVFNIVPGLGEEAGNALLHH
                     SAINHFSFTGSPEVGTLVQQAAAKNAVPVTLELGGKSPQVVFADADLDKALPFLVNAS
                     IQNCGQTCSAASRVLVEHSIYEDVTARLAEKFCALQAGPAFSDLACGPLISPEQKFRL
                     EKYLVEAKADGLNFAAEGVIVADAPSGGNYVKPLLIRDVPPDHAVAQEELFGPVLAVM
                     PFQDEEQAVELANGTPFGLVSGVWTQDGARQFRMAKKLKSGQVFINNYGAGGGVELPF
                     GGVKLSGHGREKGFEALYGFSVTKTIAIHHGE"
     gene            11536..12282
                     /locus_tag="EYO68_09500"
     CDS             11536..12282
                     /locus_tag="EYO68_09500"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIC08073.1"
                     /translation="MRLNNKIALVTGAGSGFGRGIAARFAEEGAKLVVADINEEAAKM
                     VAGEIGDSAIAVVADVSKNSDVEAMIQQTVAQWGRLDILVNNAGTTHRNKPMTEVTEE
                     EFDRIFAVNVKSVFLTARHGVPLMKEQGHGMILNVASTAGLRPRPGLAWYNTSKGAMI
                     TATKAMAIELASFKIRVNAINPVAGETGMLHLFMGEDTPEKRAQFVSSIPWGRLSQPQ
                     DMANAALFLCSDEADMVTGTCMEVDGGRCI"
     gene            12369..12995
                     /gene="thrH"
                     /locus_tag="EYO68_09505"
     CDS             12369..12995
                     /gene="thrH"
                     /locus_tag="EYO68_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HIC08074.1"
                     /translation="MLSHKKMTVACLDFEGVIIPEIWQGLARLCNIDDLKLTTRDIAD
                     YDELMRYRLKICDQYNLTLRDIHKVVEQMEPLDGAFAFLTWLRQRYEVIILSDTFREF
                     VEPLMHKLQYPTLFCHSLKLDKDLRIVDYCLRQKDQKRHAVKALKAINFHTVAVGDSY
                     NDISMLNEADHGIFFRPTKKIITEYPEFPVTNEYAELKIALERLTKDE"
     gene            12988..13758
                     /gene="rlmB"
                     /locus_tag="EYO68_09510"
     CDS             12988..13758
                     /gene="rlmB"
                     /locus_tag="EYO68_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIC08075.1"
                     /translation="MNRKKGAKLKERTELLYGIQPVLGALQHSKRLLNQLYLKKDADS
                     SERLREIRILAEKIKLPVSEVPVSQLTVMCPDAVHQGVVLRCGFLPFTSMPDFPQSAV
                     ATQPLLVVLDQIEDPHNLGAIIRTCGFFGVNAVVVTQDHSSGLTPVASKASAGVLEWL
                     PVISVTNLARFLNEQKTKGFWVAGLEGEAPDCFTDFSRDRPLILVLGNEGRGIRRLVR
                     KHCDWLVSIPGNHEVSSLNVSNAAAVALYHLHTLPQTP"
     gene            13941..14381
                     /locus_tag="EYO68_09515"
     CDS             13941..14381
                     /locus_tag="EYO68_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08076.1"
                     /translation="MPRFRLETALHVRERLEKLYQKALAEQVQVEQQYRDRKQLMEET
                     LNHHHKDLDEAKTQGVTINQLLMGDRFQERIERQLELTQDQLNEQLDIVELKRHELTV
                     ATQKKRVLEILKEKEIKKFRDETERLERLEADEIAQNYRRYSYN"
     gene            14446..15045
                     /locus_tag="EYO68_09520"
     CDS             14446..15045
                     /locus_tag="EYO68_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08077.1"
                     /translation="MNKLLNFTNNFKFLCLLFMFVMMARPAYSIPTTIDEIKQVFGAL
                     CADYGLEFCASEPDAGKKTKKPHKFTESEKEILTRLAEQQSRLRLRALELDRREGQLK
                     TLQEDIQRQVSQLEKLQQEIEKDIKKKKNQDNTHLQKAVALYSKMDAAIAAQSLAKLD
                     RMIAVSILKQMKEKQASLVLSSMGAEESASIIEEITRKK"
     gene            15056..>16447
                     /locus_tag="EYO68_09525"
     CDS             15056..>16447
                     /locus_tag="EYO68_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08078.1"
                     /translation="MEAIGQKAVLSSTDEVIISDELGVNSGEMGEERIFDVTLDEILA
                     EHPLDESLLEGLLEEDSLTASLASSILTQNQANPEFVEDIFGTAELSTIPAEVLDDNL
                     WTEETHTARSKVLNKSEPFPQELIEVLDPRVVSDENTFQNLPSQGVAETGLQSILQES
                     DLENPESLLQTVFHQAKPPEEGAEFSEDDLQSVMQKNRNLPVQSEEDTEYSVLQKILH
                     QVKPPEEGAEFLEDDLQTVLQTNTGQQIPIKENNSVVQGPEPVQENLEGLLQKELQTV
                     FHQGLQGNGKDATKPVAENLVKIMPAEIPPAAEKISPEQSLQAENLVKIMPAEIPPAA
                     EKISPEQSLQVVANTKTEKSLLAADNFKEAEEFEGGVKSKTTAGLQMTDVENLSDSAE
                     KVSILPTEKLKAVESLKVVDNIKTIETLQTKTSETLQTKTAEVLEGGVKSKMTAGLQM
                     TDVENLSDSAEKVL"
BASE COUNT         4894 a         3320 c         3507 g         4726 t
ORIGIN      
        1 aatcttctta atgtgaattg accgtccgag ctgcacctca aactgaactt ctgacagatc
       61 taattctggt aattgaagat ccagaagtgc tgtaccacca gaaatccgaa ttcttgcatt
      121 tttcaacttt cccttaatag ttccttcggg aaactttgtt ttttgttgct gcagtgcatt
      181 aaaattttcg atatgcgctg agagtgaaag aaaacctgaa acaaaagtat atggaactaa
      241 agaaaataaa ggaacccgtt cgatctgtac tgaatcaatg gagagatcga gtattttctg
      301 tgtcagcaat tcagtggaaa tccggaaggc tccctcatga acctgcccat gtactttagc
      361 ttttcctgat gtgatgatgg agaaaaaagg aacctctaaa ttttcaagaa ttaacaggcg
      421 aatatccgga attccagcca accattttgg ggtacgcagg acaatctcag gggtactgat
      481 tttgctccat cccaactcaa tatcctgaat ttcaaatgga attcctgttt gtgaggttaa
      541 acgaaagcga actgcatctg ctattgattt gccggggaaa ttttgtttaa aaccccaaaa
      601 agcaagaacc aaaatcactg ccattcccac taacaaaaac gaaatatgtt ttatgattcg
      661 cattgatagt catcctccca gttgcttgtg tacctgaaac gaggctcgta cctgacggtt
      721 accggggctg atgctgatac tgagatgaga agtccgtagt acaggacggt gattttcgag
      781 ttgataaagc atagacatta gatgatcaag tttaagtctg tctaaaccta ctttgacacc
      841 ctcagttccc ttttgaacat ttgttagtac attgagttgc aaattgtcct gaatttcaag
      901 ggatcgtgca atcttttcca caaaagagct gagagcctgc ggcatcacag acgcagtggc
      961 ggagcgctgt acatccttta attccctgga taggaagcgc acttgtttta tttgctgctc
     1021 cagcttgcct agttgttgaa gcagcatttt ttcctgacgg ttaagctgct gtatccccca
     1081 cattcccgca agtacaaata taatcaggaa catcgaggat atcagaagtt gttcccgcat
     1141 tgacaaattt ggcatgggaa gcttctattg aggtaggtag aaatcagtta cgttgactga
     1201 gagatacata atttcgctca gtttgtcctg tataaatctg gcgtggtcgt ccaatttttg
     1261 agattccatt ccgcatttcc agccaatgtg caatccagcc tggcagacgt cccagagaaa
     1321 aaaggactgt aaacatgtct gttggaaagc caagggcttt atagatgatt ccgctgtaaa
     1381 agtccacatt tggataaaga ttccgttcaa taaaatattc atcagtcaat gctgcttcct
     1441 caagttgctt tgcaatatcc aacaaaggat ccggagttcc aagcagatct aatattttat
     1501 cgcagtaacc tttcaggata gtggctcttg gatcaaaatt tttatacaca cgatgcccaa
     1561 atcccatcaa gcgaaaacct gaacctttgt ctttggccag ttttacaaat ttttgtacat
     1621 caccaccgtc attatgaatt tgtttcaaca tttccaaaac cgcctgattt gcccctccat
     1681 gcaacggacc ccaaagtgcg gcaataccgg ttgaaattga ggcaaacaaa tttgcttcac
     1741 tgctgcccac caatctaact gtggaggttg agcaattctg ttcatgatct gcatgtaaaa
     1801 tgaaaagtac attaagggct ttcacgaatt cctctggaac ttcataattc gtggtaggta
     1861 caccaaataa catattcaga aagtttttag tataagtgaa ttcgttaatt ggataaacaa
     1921 aaggctggcc gatatttttt ttgtatgacc aggctgcgat ggtggggagt ttgcccatca
     1981 gacgccaaat cgccaaatct actgcatcac tgtcggtagc actgaaatca ggatagaatg
     2041 ttgaaagtgc acagacgatg cttgaaagaa ttgccatcgg gtgagcgtcc ttgggaaagg
     2101 cctgataaat gtgcctgaca tcttcgtgga gcaaagtatg ctgtttaaca ttgtattcaa
     2161 agtcattcag ctgctgattt gtaggcaatt cgccataaat caataaataa ctgacttcaa
     2221 ggaaatttga tttttctgca agttgttcaa ttggataacc ccgataacgc aaaataccat
     2281 tttcaccatc caggtatgta ataccacttt cacaagatcc tgtattaaca aagcccggat
     2341 caagagttat tgcttttgtt tgagaacgta atttactaat atcaatgcca atttcacttt
     2401 cactccccat aaccagtggg agatcaatag atttttcatt gacttcaagt cttgctgtat
     2461 tcgccatgct gcctcttttt agctaaaatg tggagaatta tagatatttc aacctcctgg
     2521 tgacaggtta tcagatcttc accaaatggg caatattttc agccttagaa gttgtagttg
     2581 ttatggaaag tatttaagga attgaccagg aaattctctt gattcactcc tgttctatta
     2641 tgacgtcttc cggtcgagtg ctggaatatc tggcttcaat ctggcgtcgc agccaggatt
     2701 tgattgcatt tctgctgctt ggaacgagga ggataaaacc tattgtgtct gttaaaaaac
     2761 ctggagtcag caacacaagt cctgctacac caattaacaa agcatcaagt agttctccag
     2821 agggcatcct tccttcacgc agactttcct ggatacgaaa caaagtttgt aatccctgta
     2881 ttcgagctaa atatgctcct aaaaaaccgg taaaaatgac gagggcaatt gaagttagtg
     2941 caccaaatac agtaccaatt tttatcagca ggtaaatttc aaaaatggga atgatagtaa
     3001 aggcgagaaa taactttaat aacataagcc taagacttgt gtgttgtgaa tatagtgtat
     3061 gaaaatcagg aagaatcgga actaaccggt gtgatactga acctgttaat tcccgaaact
     3121 gcctgtcttc cactggcttg acgaccaatc acacgccagt aaagctgatt tttctctttt
     3181 gctctcatca atcccatcgc cattcccgga agttctcctg caagcggaac cagttccttg
     3241 tcagcaatcc agcggtttcc ctgcggtaga tcaaaatata atccggagtc ttcaaatttt
     3301 tcatcaacac caacttgaat tttgaattca ctgaaagcca gactgtccca ctgaaaaaga
     3361 aaaagcccat tttcttcaaa agtgaatttg tcagtaggta aaaggagctt aatatcactg
     3421 agtttgatta cgttggattt tgcagcatta tttttttcct tcagttcttc aatttcgtta
     3481 tctggatcaa cagtagcaat aatttgatat ttgcccaaca attggtcatc cggaaggatg
     3541 tattccaagt caaaagttgt gatttctccc ggtgccatca cgtgtacact gggcatggct
     3601 attacagact ttcgcattgg ttgtttgaca ttataatatt gaaccttagc taaaatatcc
     3661 ctgacaatat ccccacctgc attcattaag ttcaaacgta atttaagctt ctcaccacga
     3721 agggcgtgct ttggaagaac ctgtacagat tcccagcgca aatcaggcgg atcaaatctc
     3781 aaaggagaga tgacgttttt cccggatact ctcacacctg acgctaattg ggtttttgca
     3841 acttcaacat cctgatgaga tgctacaaaa aaatatctct catctcctgc agttgtattg
     3901 attaatatcc gtttgaattt agcatcccaa actctaaccg agatttgata aaggccggga
     3961 tcacccaaat caatatcatg cagtacccgt gttgtgtcac gagcctgaat cgtataaact
     4021 ttgcgaagac cctttttttt caattcctga ttgtcaactc gaacttttat ttctacgcct
     4081 actttaattt gaatcttagc atgattggca attgcagttt gaaccaactg tgtatcacca
     4141 tgttttgggc agtctggatt gtttacagat ttaataatgg aatccggaga gtcaattgtc
     4201 atatccgggc catcaggtaa tggacaggtt ttagaaagga caattgcaat gatttttaga
     4261 tcaggagact gatttgcaga caacaaggtc ggtaaaaata gaacaattat cccggaaaaa
     4321 atcagtttca acagtaaaat ccggaacatt ttataccagg ttatttaggt ttatcaattt
     4381 tctattgatc tgtttttgca gggaaatgtc ttgaaacagg aaaacgtttt tcgtaagaaa
     4441 tttgtaaaat cgcaaatttc atacatttta tattttcagt cagctgggaa atgcctggcc
     4501 ttcattgcac aagcttaagt tgattaaact gtacttccac ccagcataat ttccctctgt
     4561 aataaatctc gccctgaaaa aatcagcttg ctttcagcag gactgtttcc tcttttccat
     4621 tagtattttg taaatggtac cagtgactga tattttgatc aaaatcatca tgaacagtac
     4681 agtgaattgc accaagctgg tcaacctgaa ctatggtgat aagatgcagt cgtaccattt
     4741 ccaaaattgc catgaacgta attataaacc acgacagagg tttgttatct tcaaacagtt
     4801 cttcagtaag acaatgcttc cgtttcccga aaagagaaat cagttcgtca atgcgatcct
     4861 cgatccgtac attttctgtt tcaattaaat gtctggttat tttaggaaga cgcttcatta
     4921 cccgcccgaa agcttccatc agagcagaaa ctgagatgtc ttcgtattcc ggtgcagttt
     4981 cctcgttgtc tgcaactaac tccacatttt ccggacgctg aaatacatcc cgtcccagta
     5041 tatcaagaga acttagttca aacgctgcat ccttgtaatg ctgatattcc aataagcgga
     5101 gccgcagaat ttcttccgga tcctcttctt ccacttcttc aatatcttcc ggagacttgg
     5161 ggagcaaata tctggacttg attaaaatca acaaagtggc aatttctaag aattcaccgg
     5221 caagatcgag attgattgtc tgcatcatct ccacatattc caaatatgag gaagtcaatt
     5281 cagagagccg tacttcttca attgagactt ctttttttcg tataagatgc agcaacaaat
     5341 ccaacggccc ctcaaataaa ggaattttga tttcgagagg gttttccgga tttgggaaaa
     5401 ttgctgggat ctctacgctt ttcttaattt gttccattgt ttccatacca tccatgtctg
     5461 gagaaaatgc agattttatt ccgtaacttt atctaaattg tcagaaagtt caggtatccc
     5521 catatcttcc agaacttggt cagatacctt gttgagttct tttgcttttt taaaccgggc
     5581 actggcgcgc tgattaactt tttccaaatc ttttttcgtc cggataatgg ttggacgaat
     5641 aaaaattagc aggttgattt tctcaaattg atccaatttg ctgcgaaaaa gccaaccaat
     5701 caagggcagg tctccaaatc caggaatctt attttgattt cgtgcaacct tttctttgat
     5761 cagaccaccc agaacaataa tctcaccatc ctctgcaatc acagaggttt taattgtcct
     5821 tttaaatatc gtaattgctt ccgcaccagg atttacaaca tcactggact cttgtgaaat
     5881 atcgagtcgt atactgtttt ctccactgat ttgcggttta atttcaagtt tgataccaac
     5941 ttccttatat tggtattctt ttgtggtaat gttactcgcg cttgttaccg tttttggtga
     6001 aacaggcaca acggaaacta cgttaatgtt aggtatttca ccctcttgag gattattgat
     6061 aatcattaat tgcggatttg caaggacatt cacatcattg ttgttttgaa ccgctttgac
     6121 gaatgcacca aaactaaaaa attcctttcc tccaatgctg attgtgttac cgttcaagac
     6181 tccgattgtg gaactatttg cagcaattgc ggcggtccct cctgttgatg catcgccggt
     6241 gaatgcacct gccccgggga aaccacctgt tatcacccgt cctccgctgg ttacaccgga
     6301 tgctttccag ttgataccta aatccaaact ttttgaaagt gaaacctcca ttacaagtgc
     6361 ctccacatag acttgcattc taggaacatc aagttcaata ataatctggt ctaaagttgt
     6421 gaaggcatca gaaggtgcaa aaatcaagag agaatttgtt gcctcatcgg cgctgataga
     6481 aatgtcagtt tgccctcctg aaccaacagt tttggactga ttttgagatt caggctttgg
     6541 tttttcatct tttctggcaa cctcagtaat tcttccggta acttctttga gcaggacagc
     6601 catatctttt gccacagcat gcttgagatg ataaagctta aaattacttt ttatttttcc
     6661 gctttcctgg aaaacgtcca actttttgat cagagataat atttgttcga tttcaatccg
     6721 tcctgcaata atgatcaacg agttcagacg ttcttcctca attattttaa caggactggc
     6781 ttttgcggtt ttaccctctc cttttccggc tactacactg tccgaaaata tctttgatag
     6841 taggacggcc atcctctttg cagaagtatt ttccagtgga accaatttta gctgagtttc
     6901 tccggatgtt ccttgtactg tgtcaagttt tgcaattagc tgcagtactc gatctgttgc
     6961 caggcgatct gcgatgatga ttagtgcatt cagtcgaggt tctgcgatta ttttgacggc
     7021 actgcctgtt gcagttttcc cttcaccttt acctgcaact attttatctg caaaaatttt
     7081 tgaaagcagc ggtgctaatg ttgtggcgta agcatattgc aatgtctgta gttttatctg
     7141 acggtttcct ctaggaatat caagttgatc taccaggtca ataatttgct gggttgtcac
     7201 tggatttgca ataattatga gagcattgaa aggtgcaaaa ggcagaattt taatcgatga
     7261 cgtttttgga aaaaccgtcg ttatgagtgg aatcagtttt gctgcatcag aatattgtag
     7321 ttcatgaatg gttgttgtag cgccttctac cggcacatca acctgttcaa ttaattcaat
     7381 tatttcagct gttgtttctc tatttgcaac aataaaaagt gaattcaagc gggcctcaat
     7441 caagagtttg aatttaggag gtttgccgtt gaggacaatg ttggaaaaca gttcagaaac
     7501 agttttgtgt atttcagtaa catcattgtg aactatttgc agcaatgtga cgatttgctc
     7561 gactccttct ggcgcagata tatcaagcat atcaattagt tctacaattc gactcacatt
     7621 cgattcaaca tcaattacaa tcaacatgtt catcggttcg taaacaagca gtgctcctgt
     7681 ctttgaaaaa attggctgta atgttgactg aatccctttc aggtctgcgt gacgaattgg
     7741 aataagacgc atgacatagt tccccccctt ggcgttcacc tttccaccat cttttgaaat
     7801 cggtaagcgt gagaaacgtg catctgcagc ggtcagtatt tccacaacct tactgccctt
     7861 tttacgtacc atagccagtc cctgactggc caatattttt tcaaagaagt aaaaggcgtc
     7921 ctctgcagat actttgaaat tacgaggagc agtgatagac atttgccctc gcatattttt
     7981 ttcttcatac acgaaaacgg tgccggtatg ttctgaaatt attttgatga attctttgag
     8041 gatcagtgtt tgaggcagat ttattgtaac ctttgagctt tttttctttt ttgctgcttc
     8101 aatttctacg gggaatgagg cctgaaacag aaagaggctt aaaaatatta ttccagacaa
     8161 ccgatgaaat ttacggtatt gctcagatag gatggaaaga aagaaaatca tgcaagacta
     8221 gagaaattaa acagtatttc aggaattact cattgatttc agtatccaat ttgcccgctc
     8281 caaatcagca ggagttgtta tttttatatt ttgtggttct ccttctactg ttgaaacttg
     8341 atcaccggtg ttttccacca gtgatgcatc gtcagtaact aaccacttat tttctattgc
     8401 ctgctttgaa gcatccagaa tcaacttagt tcgaaatcct tgcggtgtct gagtggcaaa
     8461 taatgcgcgc ctatccagaa cctccgtttt ttcctcagta attcggcgga tagtgtcctc
     8521 taagggcaaa accggaattg cggcattatg ttcatgtgcc gcatctataa ttcgatcaat
     8581 tagtttttcg ctgcaaaacg gccgagctcc gtcatgtacc aagaccagtt ccggaacttg
     8641 ttcatcctgc ataatttcca acaaagcatt gtgaacagag tcttggcgac gactgcctcc
     8701 ttcaaccaga acaatttctt ctggaatcat gttttcaaat atttgacgat cctgtgagga
     8761 aacggtcaaa tatatcttcc ggattctggg atgctttata aatgtttcaa ggccgatctc
     8821 aagtattgta cgtgttccta aaggaagaag caatttattg tgcttcgctc ccatgcgtgt
     8881 tccttgtcct gcggcaacaa ttacagcaac cgtatcctgc tcagccattt tgtgcttaag
     8941 aatttaaaga ttgagaacat cactacattg acagtatatt tttcaaatat ctcaactgcc
     9001 tagactaaga aagtttggta ctaaaagtcc agataaaaca gtgaaattct ttgacaaatc
     9061 aaaatgaagt gtatatcaga cttgtaaaaa agatcacaca aactaaacta agtgctaaca
     9121 attggacaga gtgttttaat aatgcaaaat aaccataaac aataaccaac aaactaaaaa
     9181 tgtcagaaaa aacagcgttt ataggactcg gtgtgatggg ctatcccatg gccgggtttc
     9241 tagccaaggc cggaaacgaa gtcaccgttt acaaccgcac cactgttaaa gcagaaaagt
     9301 gggtcgagga atatggtggg aaactagcac ttactccagc ggaagcatca aaggatgcag
     9361 ctttcgtttt tgcatgtgtg ggcaacgata atgacctgcg ttccgtaaca attgggactg
     9421 acggtgcatt tacaggtatg agttccggaa ccgtttttgt ggatcacact actgcttccg
     9481 ctaaaatagc acgtgatttg gctgatgcag ccaagctgaa ggaatgtgca ttccttgatg
     9541 ctcctgtttc cggaggacag gccggggcag aaaatggtgt tttgaccgta atggtgggtg
     9601 gcgatcaaga cagttttgat caagcagaac cgctgattca gaattatgca aaaaaggtca
     9661 aactattggg tcctgtgggc tacggacaat tgactaaaat ggtgaatcaa atttgcatcg
     9721 caggacttgt gcagggcttg gcagagggca tacatttcgc taaagcagtc ggcctggatg
     9781 ttgatacagt gattgaaact atttcaaaag gtgcagccca gtcctggcaa atggaaaacc
     9841 gttacaaaac catgaacgaa ggaaaattcg attacggttt tgccgtggat tggatgcgca
     9901 aagatttggg aattgcttta gaggaagccc gtgcgaacaa agcacattta ccggtgacag
     9961 ccttggttga tcagttttat tccgaagtgc aggcactcgg tgggagccgc tgggatactt
    10021 caagtttgat tgcccgcctt gaagcgatgc agcagatgaa caataaataa tttgagtaga
    10081 aaaattgcat aaatttaaag gaaaaattct cattgaagga aaatggcaaa ctccggattc
    10141 aggcgaaaca ttgcctgtaa tagatccgag tgacggctgc gagtttggac gaattgcaag
    10201 gggttcagca gtggacattg accttgcagt taaatcggcg caaaacgcaa tgcaaggtga
    10261 gtgggggaaa acgattccgg tggaacgggg gcgtttgctg caaaagctga gtagcttgat
    10321 tttggagaat gaagacgaac tgactgaaat ggaagcacgt gatgtaggga aaccgcttcg
    10381 tcaggcacgt gtggatgtca aagcctgtgc acgttatttc gaattttacg gcggggcggc
    10441 agacaaggtt cacggtgaaa caatccccta tcttgaaggc tacacagtac taacgctccg
    10501 tgagccgcac ggagttaccg gacatatcat tccttggaat tatccgctgc aaattcttgg
    10561 acggaccatc ggtggtgctt tgacaatggg taatgcctgc gtgctcaagc ctggtgaaga
    10621 agcatctcaa acagctctga tggttggaga attggcgatt caagcaggat tccctccggg
    10681 agttttcaac attgttcccg gacttggaga ggaagctgga aacgcattgc ttcatcattc
    10741 cgcaataaat catttttcat tcaccggttc tccggaagta ggaactttgg tgcagcaagc
    10801 cgctgccaaa aacgcagtac cggtaacttt ggaactcgga ggaaaatcac ctcaagtagt
    10861 ttttgccgat gcggatttgg acaaagcttt gccttttctg gtcaacgcca gcattcaaaa
    10921 ttgcggacaa acctgctctg cagcaagtcg ggttttggta gaacattcga tttacgagga
    10981 tgtgacagca cgtcttgcag aaaaattttg tgcccttcaa gccggaccgg cattcagtga
    11041 cttagcttgc ggcccgctca tcagtccgga acaaaaattt cgccttgaaa aatatctggt
    11101 agaagcaaaa gcggatggat taaactttgc tgcagaggga gtaattgttg ctgatgctcc
    11161 ttccggtggg aattatgtaa aaccgctgct gatccgggat gttcctccag atcatgcagt
    11221 tgcacaggaa gaattatttg ggccggtttt agctgtgatg ccttttcaag atgaagagca
    11281 agcggttgaa ttggcaaacg gtacgccttt tggtttggtt tccggagtct ggactcagga
    11341 cggagcacgg cagtttcgga tggccaaaaa actcaaatcc ggacaagtct ttatcaacaa
    11401 ctatggtgcc ggtggtggcg tcgaattgcc tttcggtgga gtcaaactaa gtggacacgg
    11461 ccgcgaaaaa ggctttgaag cactttacgg gttttctgta accaaaacaa ttgccattca
    11521 tcacggagaa taaatatgcg tctaaataat aaaattgcac ttgtaaccgg agccggttcc
    11581 ggatttggac gaggtattgc tgctcgtttt gctgaagaag gagcaaaact cgttgtagca
    11641 gatattaacg aggaagcggc gaaaatggtt gccggtgaaa ttggagacag tgcgattgca
    11701 gttgttgcgg atgttagtaa aaattcggat gtagaagcaa tgattcaaca aactgttgca
    11761 caatggggac ggcttgatat cctggtcaac aacgccggaa ccactcatcg gaacaaacca
    11821 atgacggaag tcacagagga agagtttgat cggatctttg cggtaaatgt gaaatctgta
    11881 tttttaactg ccaggcacgg agtaccgctg atgaaagagc agggacacgg tatgattttg
    11941 aatgttgcct caacggcagg cctgcgtcca cgcccgggac tggcctggta caacacttcc
    12001 aaaggcgcaa tgattactgc aaccaaagcc atggcgattg aactggcatc atttaaaatt
    12061 cgtgtcaacg ccataaatcc tgtggcaggg gaaacaggaa tgctgcatct ttttatggga
    12121 gaagacacgc ctgaaaaacg agcacaattt gtttccagta taccgtgggg acgtttgagt
    12181 caaccgcagg acatggcaaa cgccgcactt tttttgtgtt ccgatgaagc agacatggtg
    12241 acaggtactt gtatggaagt tgacggcgga cgttgtattt gaaacactgc tgcaggataa
    12301 ttacattttt caacgtcaat gaaccctgta aaatagctat aaattttacc tttaccaaat
    12361 aaatacaaat gcttagtcac aaaaaaatga cagtagcctg tcttgatttt gaaggagtca
    12421 taattcctga aatctggcaa ggcctggcga gactatgtaa tattgatgac ttgaaactga
    12481 cgacccgtga tattgccgat tatgatgaat tgatgcgcta ccgcttaaaa atttgtgacc
    12541 agtataatct tacacttcgg gacatccata aagtagtgga acaaatggaa ccgttggatg
    12601 gtgcatttgc atttttaaca tggttgaggc aacgttatga agttataatt ctatctgaca
    12661 cttttcgtga atttgtagaa cctttgatgc acaaacttca gtacccgacc ttgttttgtc
    12721 attcattgaa attggataaa gatttgcgaa tcgtagacta ttgcctgcgt caaaaagatc
    12781 aaaaacgcca tgctgtgaag gcgttgaaag caatcaattt tcatacagtt gcagttggtg
    12841 attcttacaa cgacatcagt atgctaaacg aggcggatca tggaattttc ttccggccaa
    12901 ctaaaaaaat cataacagaa tatccggaat ttcctgtgac caatgaatat gctgagctaa
    12961 aaattgcact ggaacgtttg acaaaagatg aataggaaaa agggtgcaaa actcaaggag
    13021 cgaactgaat tgctttatgg aatccagcct gtgttgggag ctttgcagca cagtaaacgt
    13081 ctgctgaatc aactttatct aaaaaaggat gcagattctt ccgagcgtct gagagaaatc
    13141 cggatccttg cagagaaaat aaagctccct gtgagcgaag ttccagtttc tcaattgacg
    13201 gtaatgtgtc cggatgccgt tcatcagggc gtggtcttac gttgcggctt tttgccgttt
    13261 accagcatgc ctgattttcc acaatcggct gttgcaacac aaccattact tgttgttctt
    13321 gatcaaatag aagaccccca taatctgggt gcaattattc gaacttgtgg attttttggg
    13381 gtcaatgccg tcgtagttac acaagatcac tcttccgggt taacaccagt tgcgtccaaa
    13441 gcttctgcag gagttttaga gtggttaccg gttatctctg taacaaactt ggccaggttt
    13501 ctaaatgaac aaaaaacaaa aggtttttgg gtagccggac ttgaaggaga agctcctgac
    13561 tgctttaccg atttttcacg agaccggcca cttatacttg tgctgggtaa cgaaggacgt
    13621 ggaattcgtc gtctcgtccg gaagcattgt gactggctgg taagcattcc ggggaaccat
    13681 gaggtttcct cattaaatgt gagcaatgct gctgcggttg cactatatca tttacatacc
    13741 ctgcctcaaa ccccctgact aatatcccta ttctaattat ctttgatgat tttgcaggga
    13801 aattgcacca caagaaactc atttgtctat cgttcgcaga caatttgcag actggatgca
    13861 aatccttcct taaattgtat gtatttgaac acaggaataa ttctctcaat tgatttaaaa
    13921 gcttaaacca aaaactaaat atgccgcgtt ttcgcctgga aacggcctta cacgtccggg
    13981 agcgtcttga aaagctctat caaaaagcac ttgctgaaca ggtacaggtt gaacagcagt
    14041 accgtgacag aaaacagctt atggaggaaa ctttaaacca ccatcataaa gatttagatg
    14101 aagcaaaaac acaaggtgtg actataaatc aactgttgat gggagaccgg tttcaggaaa
    14161 gaattgaaag acaactggaa ctcacgcaag atcaactaaa cgaacaactg gatattgtgg
    14221 aactgaaacg acatgaattg accgtagcca ctcaaaaaaa acgggttctg gagattctca
    14281 aagaaaaaga aatcaaaaag tttagagatg aaacggaacg tttggaacgt ttggaggcgg
    14341 atgaaattgc ccagaattat cgacgatatt cttataatta atatattttt tgcctaatat
    14401 ttacagattt tcatacttaa aatcttttac aacatcagga atgtaatgaa taaattatta
    14461 aattttacta acaatttcaa attcttatgt ttattattta tgtttgtcat gatggcaagg
    14521 cccgcttata gcataccaac caccattgac gaaattaaac aagtctttgg ggcactgtgt
    14581 gcagactacg ggcttgaatt ttgtgcatct gaacctgatg caggaaaaaa gacaaagaaa
    14641 cctcacaagt ttactgagag tgagaaagaa attctgaccc gacttgcgga acagcagagc
    14701 agattacgtt tgagagctct ggagttggac cggagagagg ggcagctcaa aacactgcag
    14761 gaagatattc aacgtcaagt tagccagttg gagaaacttc agcaggaaat tgaaaaggat
    14821 atcaaaaaaa agaaaaacca agacaatacg catctacaaa aagctgttgc tttgtactca
    14881 aaaatggatg cggcaattgc tgctcaatca cttgcaaaac ttgatcgcat gattgctgtg
    14941 agcatcctga aacagatgaa agaaaaacaa gcttcattag tactttccag tatgggcgca
    15001 gaggaatctg caagtattat tgaagaaata acaagaaaaa aatgagttag tgattatgga
    15061 agcaattgga caaaaggcag ttcttagcag tacggatgag gtaattattt ctgatgaact
    15121 gggtgtgaat tccggagaaa tgggcgaaga aaggatattc gatgttactt tggacgaaat
    15181 ccttgcagaa catcctcttg acgaaagcct gcttgaggga ctcctggaag aggatagtct
    15241 gactgcctca cttgcctcaa gcattttgac tcaaaatcaa gctaatccag aatttgttga
    15301 ggatattttt ggtacagcag agttgtcaac cattccagcg gaagttttgg atgacaatct
    15361 ttggactgaa gaaactcata ctgctcgatc aaaagttttg aataaatcgg aaccttttcc
    15421 acaagaattg attgaggtcc ttgacccaag ggttgtctca gatgaaaaca cttttcaaaa
    15481 tctaccgagt cagggtgtgg cagaaactgg gctccaatcg attttgcagg aatctgattt
    15541 ggagaatccg gagtcattgc tgcaaacagt atttcatcag gcaaagcctc cggaagaagg
    15601 tgctgaattt tcggaggatg atttacagtc tgtcatgcag aaaaatagaa atctgccagt
    15661 tcagtcggaa gaagatactg aatattcagt gttgcaaaaa atattacatc aggtaaagcc
    15721 tccggaagaa ggtgctgaat ttttggagga tgatttacag actgtccttc aaacaaatac
    15781 cggacaacaa attccgataa aagaaaataa ttcggttgta caaggccccg aacctgttca
    15841 ggaaaatctg gaaggacttc ttcaaaagga attacaaact gtttttcatc aaggattaca
    15901 ggggaatgga aaagatgcta caaaaccagt agcagaaaac ctggtaaaga ttatgcctgc
    15961 agaaatacca ccggctgcag aaaagattag cccggagcaa agcctgcaag cagaaaacct
    16021 ggtaaagatt atgcctgcag aaataccacc ggctgcagaa aagattagcc cggagcaaag
    16081 cctgcaagtt gtagcaaata ctaaaactga gaaaagctta ctggctgcag ataatttcaa
    16141 agaagcagaa gagtttgaag gtggagtcaa atcaaaaacg acagcaggtt tacaaatgac
    16201 tgatgttgag aatttgagtg acagtgctga aaaggtgtcc attctgccca ctgaaaagct
    16261 gaaagctgtg gaaagtctca aagttgtaga caacataaaa acgatcgaaa cgttacaaac
    16321 taaaacttcc gaaactttac aaactaaaac tgctgaagta cttgaaggtg gagtgaaatc
    16381 aaaaatgaca gcaggtttac aaatgactga tgttgagaat ttgagtgaca gtgctgaaaa
    16441 ggtgtta
//
LOCUS       DTVD01000255            4539 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6728_length_4539_cov_125.418153, whole genome
            shotgun sequence.
ACCESSION   DTVD01000255 DTVD01000000
VERSION     DTVD01000255.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6728_length_4539_cov_125.418153"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(213..632)
                     /locus_tag="EYO68_09530"
                     /pseudo
     CDS             complement(213..632)
                     /locus_tag="EYO68_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
     gene            complement(616..864)
                     /locus_tag="EYO68_09535"
     CDS             complement(616..864)
                     /locus_tag="EYO68_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIC08079.1"
                     /translation="METAKLFMNGRSQAVRLPKKFRFEGNEVLIKKTNAGVLLIPKTD
                     SPWEVWKSNLMKYDTPFMEERKQPVELPERKGLNEVFD"
     gene            complement(1305..1517)
                     /locus_tag="EYO68_09540"
     CDS             complement(1305..1517)
                     /locus_tag="EYO68_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08080.1"
                     /translation="MQKLADKNFQLLKTNTSHPSLHFKKIGQSKQLWSVRIGLQYRAL
                     GREKPEGIVWIWIGLHDEYEKLLKKR"
     gene            complement(2342..2545)
                     /locus_tag="EYO68_09545"
                     /pseudo
     CDS             complement(2342..2545)
                     /locus_tag="EYO68_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2542..2760)
                     /locus_tag="EYO68_09550"
     CDS             complement(2542..2760)
                     /locus_tag="EYO68_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08081.1"
                     /translation="MTDLLETTFKEAARLPDVEQNIFARQMLEELVSERNWTQLFVKS
                     ENVLDRLADEVLAEFEQGKTLPLKIEQM"
     gene            4250..>4539
                     /locus_tag="EYO68_09555"
     CDS             4250..>4539
                     /locus_tag="EYO68_09555"
                     /inference="COORDINATES: protein motif:HMM:PF03400.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HIC08082.1"
                     /translation="MAFFTVKKNKKWIIKALDRHTRRTVAWVVSGRNATTARKLYNKL
                     SHLKDCTFYTDDWDAFTKVLPRDRHIIGKKHTISIEQDNSNTRHHLGRMTRRT"
BASE COUNT         1358 a          910 c          890 g         1381 t
ORIGIN      
        1 tcttacgtgg cggggaaaca aaatagggga gaataattct gaacggtttg tggtgcggta
       61 tggggaacgg tatgatgaac ggttgcacct ttctgcgtgg cggagaaaca caatggcgga
      121 gaataattct gaacgattgc accgtccttc ggttttgatg aaggagagca ggggtatcca
      181 tgttctctac tctaccttta tctttttgtg atttagttaa tttttgtgat gtgagaagtc
      241 cagttttcga ttttcaggcc gcgaattctc ttgaattcct tttcattgtt ggtcacaagt
      301 atccggtttt cgctcaaggt atgggccgca attaagagat cgaattgacc gattatttcc
      361 ccttttcttt ccagttcggc acgaatttct ccatataaaa cagttgcctt ttgagagaaa
      421 ctcaaaatat ccaacggtac aaggaattca tgaagacgat gttgattttt ttgcttttgc
      481 cgactatttt aaactccata ttgaagctca gagacagtga ttgatgaaac tccgatttct
      541 ccgacttcaa aagagttaaa cttgttaatt atttcaatag ggtgttgatt cataatgtaa
      601 atacagatat ttgtatcaat caaatacttc attcaacccc ttgcgttccg gcagttcaac
      661 aggttgcttc cgttcttcca tgaaaggagt atcatatttc atcaagttgc ttttccacac
      721 ttcccaaggg gaatccgttt tgggaatcaa caagactcct gcattcgttt ttttgattaa
      781 cacttcgttt ccttcaaaac gaaatttttt cggaagtcgt acagcctgac tgcgtccgtt
      841 cataaataat tttgcggttt ccatagtttt ctccaaatgt tgagacatat ataaataagt
      901 atatatcatt caagatttta atcaaggaaa aaatcgggtt atgatcaaat ctgttaaagg
      961 tcgaaagaac accgacattt taatgagcgc ttttttcttc ctaaagaatc cattaaggct
     1021 gcccatcctt tgtaagtaca ataatccgcc ccgcgtaggt ctcacctcca caaaaaaaga
     1081 agaaatataa gtcagggaac aagggcagga ttggaattta gcacttcctg atttgtgtca
     1141 taattcattg tttaggagcc atgctgactg cattccgcgg cagagcaaag taacgagttt
     1201 atagatattg taaagccgtg tacgggaaaa ccggatttac ggtttgatga aggggagggc
     1261 aggggcaact ttgtcccctg ttctacctct tgtcttttca tctttcaccg ttttttcaac
     1321 agcttttcat attcgtcatg taaaccaatc caaatccaca caattccttc tggtttctca
     1381 cgtcccaggg cccgatattg taaacctatt ctcacagacc ataattgttt agattgccca
     1441 attttcttga aatgtaacga aggatgtgag gtattggttt ttagaagttg aaaattctta
     1501 tcggcaagtt tctgcacttt gtcaggaagt gcctgttagt cttcccaaaa acgtggcagg
     1561 gttctgtgct tcaaagtgga cctgataatc cagcttcgta ctcggcatcg acttcagcca
     1621 gaaaattgtc caaacgccct gcttccaaat ctttctcgat ttgctggtct cccatacagt
     1681 cgcctgatac ttttcaaacc aacttgataa ggcaaataat ttacttgctg gtaactcagt
     1741 gattgcggct tctatttctt taaaactcac tgcttgctcc tgttttatag attgtcacaa
     1801 aagtttatca aaaataataa gactttctag ccacaagtaa aagcataaaa ctcaaagtct
     1861 ccgttaaacc agtgctcaga gttacattcg acaagccagg atacagtaaa aaacaaaaaa
     1921 gccgccctat tccacattca atacgaggcg gctcaaataa tcaatatttt gtcaatttag
     1981 cttcttgctt aatcggtagc cccggatggt tcttgagcgc actgcactga gcttgtctaa
     2041 gggaagggga gtcgaaaggc cgttccgggg aatcgcaaag gcggagaata atttctgaac
     2101 ggtggcccct tcctccgtgg cgggtaaaca caacgccaga gaataattct gaacggtagc
     2161 ccctgactct aaaattccgc aaatcctaaa actcagatta agatagaata tttcggtttt
     2221 cagcattgaa aatcttggga acaatgcagt ccaacaacaa agctctatga tagttccttt
     2281 attattttaa tattttttcg tattctgagt gtttccctat ccaaaaccaa acaatttcgc
     2341 tttaactcaa tattcctact gctctgtaat tttttgaaat tcgcactgaa tacattggac
     2401 gtttggaatg cacacgcttg aagttcagac ttggatgata ggggtctgct tgaaattacc
     2461 tgtatgcaga ttgggcctgt cttttctgca ctttgggtaa agccgataaa agttgtctga
     2521 atgtttttgt tgtatgtgaa atcacatttg ttcaattttt agaggaagcg tttttccttg
     2581 ctcaaattcg gcaaggactt catcggccaa gcggtctaaa acattttccg atttaacaaa
     2641 taattgtgtc caatttcttt cagacaccaa ttcctccagc atttgacgag caaatatatt
     2701 ctgctctaca tcaggtagtc gagcagcttc cttaaacgtt gtttcaagaa gatctgtcat
     2761 catatttcta aaataaaaaa tcatttactg gcagtatatc acacttccag gagtacacta
     2821 aaacaaaaaa gcaattccat tccaaattga aaaatggtga ccaaacttaa taatgttttt
     2881 gtgtccttat ttgtttacta tttaatacgt cattcagggc aaatcgcagt gttgaccagg
     2941 aatccagggg tgctgatgca aatcttgtct tcaaactgat tgtgtgtctg gttccaggat
     3001 caagtccggg atgacaaaat aaaaacggac ctatgtcaca tagagtgggt ttatgatgag
     3061 gacagccccc gaatttattc gggggctatg agtcatctta aaaacaggaa accgtttcaa
     3121 cggttttgta gggaaaaggc atgccttgtc tctacgtaca gcaaaggtgg aatcgtttat
     3181 atttgaaacc cctcgttacg aggctccgcc ttgtgacgct gtctgtgaag ctctgccaca
     3241 tgaatgatgc tttacacaac acgcaaggcg gagccttgct gacttcattc cgtggcaaag
     3301 ccgcggaaag agtttaaaaa agtttaaata attgcagagt ctcttgttta ccgataacca
     3361 ccgcaggggt gcgtgttaat acccgacacc tttatcttat ttccccgata attgccatac
     3421 ctattctacc ggttccagta ccatttcctt gataatcgga ataactcaga acaaatgtgg
     3481 taaaggagct ggaattatca agaatggata tagatttctg ttgatatgtt ctaccggcat
     3541 taaacagact ttccgtagca aaagttattt gatttccatt tgaaataatc gcgcttctcg
     3601 cataaccagc atgtgtatta ggtacaggtc cattatcata agctaccaca atttgggtag
     3661 gactaataac agcaacattt tgattatcaa tagcattatt agtaaaggca tattctgaac
     3721 cgaaagatat atcatttcct gaaactgtac cgattaattc tttgccataa gatttataag
     3781 ttgcgccatc tgatccattt tgataggaat cattataaat tattataaag tgggtagaat
     3841 caagaactgc aagatgggca ttaactgaat tgagagtgtt tacatttaga tagatttatg
     3901 gtataataca atttttaacc caatatcagg attcattcat ggaagaatgt aaaaattgta
     3961 aaagtaccga aactgttaaa aatggtcttg tacgccaaaa gcaacgctat aaatgtaaat
     4021 catgcggcca caactttgtg tgcggtgatg aacgcaggaa aaaatcaaca gagttaaaac
     4081 gaatcacgag tgtattgtta tactcgcttg gaaaagccag ctttcgtttt ttagctaaac
     4141 tctttgatgt ttctccaacc acgacctata actgggtgcg tcagaccgca gaatcgtttg
     4201 gcgaaccagt ggttgatgaa aatattaaag aaatcgaaat cgacgaaatg tggcattttt
     4261 tacagtcaaa aaaaacaaaa aatggattat caaggccttg gatcgtcaca caaggcgaac
     4321 tgtcgcctgg gttgtcagtg gtcgtaatgc tacaacggca cgaaaattat acaacaaact
     4381 ctcacatctg aaagattgta cattctatac cgatgactgg gatgcgttta ccaaggtttt
     4441 gccaagagat cgccacatca ttggcaaaaa acacacgatt tccattgagc aagataattc
     4501 caacaccaga caccaccttg gaaggatgac acgaagaac
//
LOCUS       DTVD01000256           16148 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_691_length_16148_cov_457.224383, whole genome
            shotgun sequence.
ACCESSION   DTVD01000256 DTVD01000000
VERSION     DTVD01000256.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 16148)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16148)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16148
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_16148_cov_457.224383"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     regulatory      195..397
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            508..717
                     /locus_tag="EYO68_09560"
     CDS             508..717
                     /locus_tag="EYO68_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB-domain containing protein"
                     /protein_id="HIC08083.1"
                     /translation="MTTNTEVAGTSGSLDTSDVGVSSQRLTLALLMLLAGAVLIFTVG
                     FAQGSGGVFHNATHDTRHAVTFPCH"
     gene            735..1532
                     /locus_tag="EYO68_09565"
     CDS             735..1532
                     /locus_tag="EYO68_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08084.1"
                     /translation="MISKAVTTVLLAGFVAGILVTGAQMLKVTPLILQAEKYEVGTEV
                     VPHTHQQSGITHEHELNGVALDVHASMKDAHAAEAVSVDHSDESWVPEDGAERTFYTG
                     ISNIVTGIAFSLMLVAVYLLRGKPVNMNSGLLWGAAGFLIFSGSPALGLPPELPGMTA
                     AALDARQTWWIGTVIATAIGIGLFSETKTILPKIAAVLLLAAPHLIGAPHPLLFESNV
                     PAELSAQFAIASLFTSAFFWMVLGASTGYFYQKLVPKTTESLTTASA"
     gene            1696..2694
                     /locus_tag="EYO68_09570"
     CDS             1696..2694
                     /locus_tag="EYO68_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIC08085.1"
                     /translation="MIRSDFLDAVGNTPLIKLRKASELTGCNILGKAEFMNPGGSVKD
                     RAAKAIVLDAEARGLLRPGGVIVEGTAGNTGIGLALVANSLGYRTVIVIPETQSQEKK
                     DMLRLCGADLREVPAVPYRDENNYVKFSGRLAEELAKTEANGAIWANQFDNVANRKGH
                     YENTGPEIFEQTNGKVDAFICAVGTGGTLAGTGMALKERNPKIIISLADPLGAALHSF
                     YTTGELSSCGGSITEGIGQGRITANLEDAPVDQSFQIPDEEALPICFDLLKEEGLCLG
                     ASSGINVAGAIRLAQELGPGKTIVTILADSGVRYQSKLFNPVFLREKGLPVPEWME"
     gene            2708..4204
                     /locus_tag="EYO68_09575"
     CDS             2708..4204
                     /locus_tag="EYO68_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIC08086.1"
                     /translation="MPNILMFQGTGSGVGKSVLAAGFCRLLKNRGFRVLPFKAQNMAL
                     NSGVTPEGLEMGRAQIVQAEACGVFPDVRMNPILLKPQGSGVSQLICMGKVVSTCSAR
                     EYYTQAEDNFRIAKQAFDSLKTEADWIVMEGAGSPAEINLQATDIVNMRMAEYAEAKV
                     MLIGDIDRGGVFAWLKGTYDLIQAQHRSLLQGMLINKFRGDVSLLESGIEQFNEIVPV
                     PVLGVIPWREMLLEDEDSQNLQSKIDPEAKLDVAIIRLPHISNFTDFDPLKQISGISV
                     RFVTSVPDLESADLIILPGSKNTLFDLRYLHEKGFAEKLNQLSGRTWILGICGGFQML
                     GEAVEDPNNMESSGASGETESSGTGGCESGLGLLPMTTVLEGDKKLVRRRYKGINWLN
                     GLDWSGYEIHLGRTEFHKNPQESIVIEEVTAANDSSLGVIDRKQKIIGTYIHGWLESP
                     EVTKKLLALLTAKTFDIPLSFQETKEREMDELALFLEEHCEVEKILAN"
     gene            4233..4844
                     /locus_tag="EYO68_09580"
     CDS             4233..4844
                     /locus_tag="EYO68_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIC08087.1"
                     /translation="MTEIIKDLATEKTVNGIKIPKRPKIKQGAFQIYTGEGKGKSTAS
                     LGLMLRALGSGMHVYYVRMLKPRWKTGELKLCPENFHPNLTFRNVPHYWALCVSKTIP
                     EDVETMKEKMEPEMEDLHEQVKSGKYDLIIADEINYCIYRGLLSVEKAIQIVEDRPKN
                     VELVFTGRYAHEELIGRADLVTEMKKIKHHFDKGIRARIGIEF"
     gene            4918..6144
                     /locus_tag="EYO68_09585"
     CDS             4918..6144
                     /locus_tag="EYO68_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC08088.1"
                     /translation="MLFARVYFPFALGYAISYFYRNTNAIIESDLVDELGLGPADLGL
                     LTGAYFFSFAIFQLPLGILLDRYGPRRTEAVLLLFAALGAWIFSKADSLSGLILGRLL
                     IGLGVSACLMAAFKAYVIWFSSGRLPMINGLQMVAGGLGALVATTPLQNVLSITDWRG
                     VFTGLAIITVFASLFLWFILPEHQSSADKHPAIKTQLKEMGQIFRSPVFWSIVPLTAL
                     SNGSFLAIHGLWIKPWLRDVVSLGEGDSTQLLFAMTLAIIAGYFSLGIFSERLSQLFD
                     IRPITVGVFGMTLFLTAQFFMAYGWATSPLLLVVLFGFLGSSCILTFAGLAQIFPKKY
                     SGRVSTILNVQVFMGAFIIQWGIGAIIELWPTTEIGYDPASYQVAIGVLVLMQAAGLG
                     WYLLSQQLIKKQPEMI"
     gene            complement(6148..6417)
                     /locus_tag="EYO68_09590"
     CDS             complement(6148..6417)
                     /locus_tag="EYO68_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIC08089.1"
                     /translation="MHPSHENQLVRLKKVEGQVRGIQTMIEERRYCMDLLSQIRAVTG
                     AMRKIESGILASHLQHCVNDAITSKSQKEATVKIKEITRLFEKLN"
     gene            6768..7589
                     /locus_tag="EYO68_09595"
     CDS             6768..7589
                     /locus_tag="EYO68_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIC08090.1"
                     /translation="MPSVTVILPTWNRAEWLETAIESVLTQTFRDYELIVVDDASTDS
                     TAEIFERYSGKIRCIILTKNRGVSAARNAAVLKSDSEWIAFLDSDDYWHPEKLQKQIE
                     QTKIRQGFPIHFTDEIWIRNGIRVNPKKKHQKREGWIFQPSLALCLMAPSTVLLRREL
                     LEVHGMFDERLPVCEDYDLWLRLCAQHPAALLNEKLMTRHGGHADQLSQREWGIDRYR
                     VQSINKILKTEILKPDDRLAAIRMLQKKCRILIQGFHKRDNMKEVRNYEKIISQF"
     gene            7610..8104
                     /locus_tag="EYO68_09600"
     CDS             7610..8104
                     /locus_tag="EYO68_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIC08091.1"
                     /translation="MTEIKTDFEIRQAEEEDVPEILELIKALAEFENLSDEVVATEEL
                     LKITLFGINSPAEVQIAYDKNKTLGFALYFRTFSTFLGRPGIYLEDLYVRESARGKGV
                     GEALLRRLAQRTREIGGGRLEWSVLNWNEAAIKFYQKMGASPLDEWTMFRVTGNKLKE
                     LAEE"
     gene            complement(8173..8658)
                     /gene="nusB"
                     /locus_tag="EYO68_09605"
     CDS             complement(8173..8658)
                     /gene="nusB"
                     /locus_tag="EYO68_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIC08092.1"
                     /translation="MRHQARSYALQALYQAELLQSKAMKHADRFLDQLETSPEIKEFA
                     RELVAGTLQHRKHLDRYLRRNLEHWKLNRLSTTVRNILRLATYELYHHPELSHSVVID
                     EAVELCKDFVDDTSHGLTNRVLQRVYDQITEERKTKAIAKKEESSKIKPDKKTQEPES
                     K"
     gene            complement(8664..9992)
                     /gene="lpxC"
                     /locus_tag="EYO68_09610"
     CDS             complement(8664..9992)
                     /gene="lpxC"
                     /locus_tag="EYO68_09610"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
                     deacetylase"
                     /protein_id="HIC08093.1"
                     /translation="MAKSQIICVVDDEPAIRETLENVLSDEGYPVMSCRDSEDFYAKL
                     EKQTPALVLLDIWLPGTDGMAILSQLRETHPDIPVIMMSGHAGIDAAVNAIKLGAVDF
                     MEKPLRLEILLDKISIVLSNKPPKKIKDLASDTLLEVAKIINPIIPSGAVQLQDSKRP
                     QRTLKGNVVLNGKGLLTGRNTGVILSPLDPNSGIVFQTLDDTSLPAHITNIENFSQSV
                     AKQSFSANSTVLARDNRLVRTVEHLMAALHMAGITNVLAKVDEEIPNIDGSAKDFSEL
                     IKEAGILDQNESAKDAVVLEPIQVGRKKLDEKHLYVEPYEGFEVKMRVDYDAPIGEQK
                     LTFNSEKDSFNSDIAPARSFNTFENIDMAQKKGTVGSGYLDSHIIMHDGKVINTDLRY
                     PDEFVRHKILDLIGDLYLLGFPLRGRVVANMTSHGYNQALVQKLHVALTT"
     gene            complement(10032..10499)
                     /locus_tag="EYO68_09615"
     CDS             complement(10032..10499)
                     /locus_tag="EYO68_09615"
                     /inference="COORDINATES: protein motif:HMM:PF02643.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIC08094.1"
                     /translation="MILRHFFFCTIFIYFWFVPFFGSNFAFAEVQDYAHAIVTTSTGK
                     KISVEVADTVEKRSLGLGKRSGLENGWGMLFVFEKRKQHGFWMKDMEFPLDIIWLDNH
                     RIAYILRNVQPAKSGVIPPVMTPPVAGNFVLEIDAGRADELKLQVGQRLKYQF"
     gene            complement(10496..11014)
                     /gene="def"
                     /locus_tag="EYO68_09620"
     CDS             complement(10496..11014)
                     /gene="def"
                     /locus_tag="EYO68_09620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIC08095.1"
                     /translation="MALLEIHTFPDSVLRQKAEPVTVFDQELDETAQSMLETMYFSGG
                     IGLAAIQAGILKQIIVIDLKSGEEDTELREPHVFINPEILEKSGTTVSEEGCLSVIEF
                     RAEVDRAEKMTIEYQDVAGYSKKLEAEDMMSICLQHEIDHLNGILFIDHLPLLKQKMI
                     KKRLTKLANDEA"
     gene            11223..12170
                     /locus_tag="EYO68_09625"
     CDS             11223..12170
                     /locus_tag="EYO68_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HIC08096.1"
                     /translation="MNTGFYTNPIYLEHDTGSHPENANRLRAIQEKLESEGLLERLIL
                     QSGRSATSQEIKLLHSEKLISAVEAAAESGARTLHTPDCIISAQTFNAAAHAVGSVLD
                     GVIEVAERRLDNAFCAVRPPGHHAENDSAMGFCFFNNIALAAEFLTREMGFKRVLIFD
                     FDVHHGNGTQHFFEERADIFFASIHQDPRTSFPGTGFAYERGSGGGAGFTLNVPVPPG
                     MGDEEYLQIFYDQVQPKLEEYKPDFVLVSAGFDAHRDDPLASLNLTERTFRELTRELK
                     QLAEQYAGGRIMSLLEGGYDLNALSSCVQEHLLILHAED"
     gene            complement(12187..13191)
                     /locus_tag="EYO68_09630"
     CDS             complement(12187..13191)
                     /locus_tag="EYO68_09630"
                     /inference="COORDINATES: protein motif:HMM:PF03706.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HIC08097.1"
                     /translation="MKKLLLILLKIVVVGTILIYLVRSGRLNFEKLMLFRDAPEILAM
                     MVAILILAVVPMATFRWWLLLRAIGIQVNPKQTFVLTWIGNFFNTTLPGAVTGDVVKG
                     YYVIKAQEEDGRTRAFMTLLIDRFVGLFGLIVMAFFALVFNFELILSQERLHSLAWII
                     VALFGGTVLFYSIVLFPFKEGRDPFLRLFQRLPASKFSIKVYSAFKSYQHQKTTLFLT
                     LFLSIGLHTLIALIFFQVAHLMGIKEMELATQFFLMPIGLITVAIPIAPGGIGIGHAA
                     FETLYQLAGLSGGADIFNLFIIVQLGVFMLGGIPYFLYSSNYQIPKNSEKMFEEEAEK
                     "
     gene            complement(13199..13717)
                     /locus_tag="EYO68_09635"
     CDS             complement(13199..13717)
                     /locus_tag="EYO68_09635"
                     /inference="COORDINATES: protein motif:HMM:PF06750.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIC08098.1"
                     /translation="MQYFEIGFWTLLGLIIGSFVNVCLDRLPLQFADKTRRLSLLNAP
                     EISTFLKQQLQDQSLNLFKPACSFCFSCGHQLKWFEKIPVLSFILSKGRCRKCKSTIG
                     FRTIWIETMHGLWYAGTAWLLQGWVWPLFYSISFSLLLILGYCWSCNQVRKTLLSAGG
                     VLVILVFCVNYF"
     gene            complement(13762..14637)
                     /locus_tag="EYO68_09640"
     CDS             complement(13762..14637)
                     /locus_tag="EYO68_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HIC08099.1"
                     /translation="MFYKPLYSQLLLILVFFTFIIGVFPLAAGDTNDKDQNIDPLLAK
                     VYKQENVSEWWVSEKLDGVRAIWNGEKLHFRSGKLISAPDWFIENFPDQLMDGELWMG
                     RGTFEKLSGIVRKIQPNHNDWRQVRYMLFELPEHPGTFTRRVRKMVKLTETLKISWLQ
                     PIPQIRLNSEDALLNMLDEIVTKGGEGLMLHRADSLYHSGRSDDLLKLKPWQDAEATV
                     VEILPGKGKFIGMMGSLLVTDESGRRFRIGTGFSNKERRNPPAVGTVITYKFTGTTKK
                     GLPKFASFLRIHQQF"
     gene            complement(14741..15532)
                     /locus_tag="EYO68_09645"
     CDS             complement(14741..15532)
                     /locus_tag="EYO68_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIC08100.1"
                     /translation="MAKVITFASQKGGVGKTTSAVHLATAFALGGYKTLLVDLDPQGS
                     VHHSLGLDKPDGLGIREVFCTPSVSSQEIIQTGKHQNLDLLLSNIQDLLTEQTVNKIA
                     ADYYHFSQWLEQNASSSYEFIIVDPPASTSSLSINAMLAANLVIVPLECEALAIKSLK
                     RFLQAFKELQRTIEPRLRLAGILLTMYNDEILTHQQICKQVYQTLGDSVFETIIPNCP
                     HILEASSLGIDVIQRHLNSAGATGYIRLANELLDRFLLPKSLEVS"
     gene            complement(15659..16060)
                     /locus_tag="EYO68_09650"
     CDS             complement(15659..16060)
                     /locus_tag="EYO68_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIC08101.1"
                     /translation="MSSALKYGMKRVVPFSYEEAVEKIKSSLKEQGFGVLTEIDVKAT
                     LKNKIDQDFTKYVILGACNPGLAFQVLSEEIDIGLLLPCNVVVYEDPEKGETVLGVID
                     PEMMVQATGRTDLDVFAKSVREKLQTALDSV"
BASE COUNT         4562 a         3332 c         3502 g         4752 t
ORIGIN      
        1 ggttaccgca cagccaattt acatgcctct tacgttcatc tttatttccc ccaggccgcc
       61 cctttgattc gagaagttct gcatttactt ccttcataat tgccttgatt gcaattgcct
      121 ggaattgagt tgattacttt acagtatttt tttacttgcc aaaaaaattg aggttgttat
      181 acttactttc cttaaggtaa tccgtaccgc aaagtaatag ttgcttttct gtgcagattt
      241 gaagggaatc ccgtgtaaat cgggaactat cctcgtaact gtaattggaa aaaataaaaa
      301 cataagccac tgaagacaga tgcaatgttt gtttttggga aggagttttt attcacttcc
      361 atgagtcagg agacctgcct tggtatttcc atacagactg ttcggggcaa atagttactg
      421 gaatcaacag cttgctgtga tcccatattt tgctgcttgt ttcgcatgtt tgtttcaaac
      481 gctaatcctt aaaacaagga gttaattatg acaacgaata cagaagtggc cggtacttcc
      541 ggttctcttg acacgagcga tgtcggagtt tcttcacaac ggctgacttt ggctttgctg
      601 atgcttctgg ccggagcagt actcattttc actgttggat ttgcacaggg ttccggtgga
      661 gtgttccaca atgcgacaca cgacacccga catgctgtca cttttccctg tcactaatcc
      721 taaagggaaa taaaatgata tcaaaagctg taacaaccgt tctgctggcc ggttttgtgg
      781 cagggatact tgtcactggt gcacaaatgc tgaaggttac tccgctgatt ctgcaggcag
      841 aaaaatatga agttggcaca gaagttgtgc ctcacactca tcagcaatcc ggaataactc
      901 atgaacatga attgaacggt gttgctctcg atgtgcacgc atcaatgaaa gatgcgcatg
      961 cagcagaggc tgtttcagtt gatcattcag atgaaagctg ggtgccggaa gatggagcgg
     1021 agcgtacttt ttacacgggt atttccaaca tcgttaccgg aattgctttc agcttaatgc
     1081 tggttgcagt ttatctgctg cgtgggaaac cggtcaatat gaactccggc ctactttggg
     1141 gcgcagccgg ttttttgatc ttctccggat ctccagcatt gggtctgcct ccggaacttc
     1201 ccggaatgac tgcggcggct cttgatgcac gtcaaacatg gtggatagga actgtgattg
     1261 ccacagcaat tggaattggc cttttttctg aaacaaaaac catcttgcca aaaatagcag
     1321 cagttttgtt actggcggca ccgcatctga ttggagcacc gcatcctctc ttgtttgaaa
     1381 gcaatgttcc ggcagaactt tctgcacaat ttgcgattgc ttcgctgttc acctctgctt
     1441 ttttctggat ggtgttggga gcatcaaccg ggtattttta tcaaaaactt gtaccgaaaa
     1501 caactgagtc tttgactacc gcctctgcct gatcaggcct tttaacttgg gtttttagac
     1561 tgtaaatctt ttaagcagtc taaattcctg aaaatctaaa ccccctccat ttttgttttt
     1621 tctctgtgtt ccggatagca tttgtgttag tatttacgat ctcaaacacc tctaatctca
     1681 actcaaaaac ctcgcatgat tcgcagtgat tttctggatg cagttggtaa tactccactg
     1741 ataaaactac gcaaagcctc tgaactgact ggttgtaata ttttaggaaa ggcggaattt
     1801 atgaatccgg gaggttcagt caaagatcgt gctgcgaaag cgattgtgct ggatgcagaa
     1861 gcgcggggac ttttacggcc gggcggagta attgtcgaag gtactgcagg caataccgga
     1921 attggactgg cactcgtggc gaattcattg ggataccgca ccgtgattgt gattccggaa
     1981 acgcaaagtc aggaaaaaaa ggatatgctg cggctttgcg gagctgattt gcgggaagtt
     2041 ccggcagtgc cctatcgcga tgaaaataac tatgtcaaat tctccggccg tcttgctgaa
     2101 gaactggcaa aaactgaagc aaacggtgcg atctgggcca atcagtttga taatgttgcc
     2161 aaccgaaaag gtcattacga aaacactggt cccgaaatat ttgaacaaac caatggtaaa
     2221 gttgatgctt tcatttgtgc agttggaaca ggaggaacac tggccggaac cggaatggca
     2281 ctcaaagaac gcaatccgaa aataattatc tcactcgcgg acccgctggg tgctgcgctg
     2341 cattcatttt acacaactgg tgaattatcg agttgtgggg gctccatcac cgagggcatc
     2401 ggtcaaggcc gcatcaccgc aaatctggaa gatgctccag ttgaccagtc ttttcaaatc
     2461 ccggatgaag aagctctgcc gatttgcttt gatcttttga aagaagaagg actttgtctt
     2521 ggagcatctt ccggaatcaa cgttgcagga gcaattcgcc tggcacaaga acttggtccg
     2581 ggaaaaacga ttgttaccat tcttgctgat tccggtgtcc gttaccaatc caagcttttc
     2641 aatcctgtat ttttgcggga aaaaggtttg ccggttccag agtggatgga gtaattgagc
     2701 tgcaaaaatg cccaacattt taatgttcca aggcaccggt tccggagttg gcaaaagtgt
     2761 tttggcggcc ggattttgcc gattgttgaa aaatcgtggc tttcgtgttt tgcccttcaa
     2821 agcacaaaac atggcactca attccggagt cacgccggaa ggtctggaaa tggggcgggc
     2881 gcagattgtt caggcggaag cctgcggtgt gttcccggat gtacgcatga atccgatttt
     2941 actcaagcct cagggctccg gtgtttccca attgatctgc atgggaaagg tcgtgagtac
     3001 atgttcggca cgtgaatatt acacgcaggc agaggataat ttcaggattg caaaacaagc
     3061 ctttgattcg ctgaaaactg aagcggactg gatcgtgatg gaaggcgcgg ggagtcctgc
     3121 ggaaataaat ttgcaggcga cggatatcgt caatatgcgc atggctgaat acgctgaagc
     3181 aaaagtcatg ctcattggtg atattgaccg cggaggtgtt tttgcctggc tcaaagggac
     3241 atacgatttg attcaggctc agcatcggtc attactgcag ggaatgctga tcaataaatt
     3301 tcgaggtgat gtttctcttt tggaatctgg aattgagcag tttaatgaaa ttgttccggt
     3361 tcctgttttg ggagtcatac cctggcgcga aatgttgctc gaggatgagg attcacaaaa
     3421 tttgcaatca aaaatagatc ctgaagcaaa actcgatgtg gcgataattc gtctgcctca
     3481 catttccaat ttcaccgatt ttgatccact caagcagatt tccggaattt cagtccgctt
     3541 cgtcaccagt gttccggatc tggaaagtgc cgacctgatc attcttccgg gaagtaaaaa
     3601 cacacttttc gatttacgtt atttacatga aaaaggtttt gcggaaaaac tgaaccagct
     3661 gagtggacgt acctggattt tgggaatttg cggtggtttt caaatgctcg gtgaagcagt
     3721 tgaagatcca aacaatatgg aatcctccgg agcgtccggt gaaaccgaat cgtctggtac
     3781 aggtggttgc gaatccggac tgggactgct gcccatgact acagttctcg aaggggataa
     3841 aaaacttgtc cggcgtagat ataaaggaat aaattggctc aatggtttag actggtccgg
     3901 atacgaaatt catttgggac gtactgaatt tcacaaaaat ccgcaagaat cgattgttat
     3961 agaggaagtt acagcggcaa atgattcttc tctgggtgtt attgaccgca aacaaaaaat
     4021 aatcggcacc tacattcacg gctggttgga aagtccggaa gtgaccaaaa aacttttggc
     4081 attgttgact gcaaaaactt ttgacattcc gctttctttt caggaaacta aagagcgtga
     4141 aatggatgag ctggcgctgt ttctggaaga acattgtgaa gtggaaaaaa tactggcgaa
     4201 ttgagcaatc aaaaaataaa cggcaaataa aaatgacaga aattattaaa gatttagcga
     4261 cagagaaaac tgtcaatgga attaaaatcc caaaacgacc caaaataaaa cagggtgcat
     4321 ttcaaattta taccggagaa ggcaagggca aaagtacagc ttcgcttgga ctgatgctgc
     4381 gtgcgctggg ttcaggaatg cacgtttatt atgtgcggat gctcaaaccg cgctggaaaa
     4441 ctggagaact gaaactctgt ccggaaaatt ttcacccaaa cctgactttc cgcaatgtac
     4501 cgcattactg ggccctttgt gtaagcaaaa caattcctga agatgtggaa acgatgaaag
     4561 aaaaaatgga acctgaaatg gaagatctgc acgaacaggt aaagtctgga aagtacgacc
     4621 tcattattgc cgatgagatt aattattgta tttacagggg cttgctttct gtcgaaaaag
     4681 caattcaaat tgtggaggat cgtccgaaaa atgtggagct ggtctttacg ggacgctatg
     4741 cacatgaaga acttattggg cgggcagatc tggtaactga aatgaagaaa atcaagcacc
     4801 actttgacaa aggaattcgt gccagaattg gtattgaatt ttgattcatc ggcctgcttt
     4861 aacgtcacta atattcttta gacccactta aatcttcaag cctgacattt taagaaaatg
     4921 cttttcgcgc gtgtttattt tccttttgcc ttaggctatg ccatatccta tttttatcgc
     4981 aataccaatg cgataattga gagtgatctg gttgatgaac ttggactcgg acctgctgat
     5041 ttggggcttt taacaggcgc atattttttt tcatttgcga tttttcagct gcctttgggt
     5101 atattgctcg accgttatgg tccgcggcgt acggaagcgg ttctgctttt atttgccgca
     5161 ttgggggcgt ggattttttc gaaggcagat tcactttcag gattgatctt gggcagattg
     5221 ctgatcggct taggtgtttc cgcgtgtctg atggcagcat tcaaagctta tgtaatctgg
     5281 ttttccagtg gacgtttgcc gatgatcaac ggcttgcaaa tggttgcagg tgggttggga
     5341 gccctggtgg cgactactcc tttgcagaat gtcctttcaa tcacagactg gcgtggtgtt
     5401 ttcacgggtc ttgccatcat tacagttttt gcttcacttt ttttatggtt tattttacct
     5461 gaacatcaaa gttctgctga caaacatcct gcaataaaaa cacagctcaa agaaatgggt
     5521 caaatattcc gcagcccggt tttttggagc attgtccctt taacagcact ttccaatggg
     5581 tcattcttgg cgattcacgg actctggatt aaaccatggc tgagagatgt tgtgagtctt
     5641 ggtgaggggg attcgacaca actattattc gcaatgacac tggctataat tgccggttat
     5701 ttttcacttg gaattttctc tgaacggctt tctcagcttt ttgatattcg cccgattact
     5761 gtgggtgttt ttggaatgac tttattttta accgcgcaat ttttcatggc ctatggctgg
     5821 gcaacaagtc ctttgttact ggttgttctt tttggatttt tgggctcttc atgcatttta
     5881 acctttgcgg gtttagctca aatttttcca aaaaaatatt ccggacgtgt tagtacaatc
     5941 ctgaatgtgc aggtttttat gggggcattt attatccagt ggggtatagg agccatcatt
     6001 gaactctggc caactacaga aattggctat gatcctgcaa gttatcaagt cgcaattggt
     6061 gtactggttt taatgcaagc agcaggtttg ggctggtatt tattatctca acaactcatc
     6121 aagaagcagc ccgaaatgat ttagcaccta attcaatttt tcaaagagac gtgtaatttc
     6181 tttaattttt accgtagctt ctttttgact tttcgaggta attgcgtcat tgacacagtg
     6241 ctgcaggtgg cttgctaaaa ttccagactc aattttacgc attgctcctg tcactgctct
     6301 gatttgggac agaagatcca tgcaatatct acgttcctca atcatagttt gaattcctcg
     6361 tacctgtcct tcaacctttt tcaggcgaac aagttgattt tcgtgacttg gatgcattgt
     6421 tgactcctca aaaaagtgaa tattaaattg ggcttgctga aagatgaaat tgttaaataa
     6481 taccaataag aaaattatag attcaggatg tcatgtacaa gggattcatc ttaaaccgca
     6541 caaaatcctt actcatatgt tgtttgattt tctcatcctg cgagttattt tcattgggat
     6601 gcggcgccgc tttcgattct tctgcattgt cattccggat taagtccgga atgacaaaag
     6661 aaaaactatt tagcaacttg tattatacct tatgggggta tcaaaaattt gtcaagtaaa
     6721 atcatggatt cagtattcat taacttgagc aattatatga ttgtgtcatg ccgagtgtaa
     6781 ctgtcatact gccaacctgg aatcgggccg aatggctgga aacagcaatt gaatcggttt
     6841 taacacagac tttccgggat tatgagctga ttgtggtaga tgatgcttca actgattcta
     6901 cagcagaaat ttttgaacgc tattccggaa aaatccggtg cattatatta acaaaaaacc
     6961 ggggagtcag tgctgcacga aatgctgctg ttttaaaaag cgacagcgag tggatcgcct
     7021 ttttggactc agatgattat tggcatccgg aaaaattaca aaagcaaatc gaacaaacca
     7081 aaattcgaca gggatttccg attcatttta ctgatgaaat atggatcagg aatggaatcc
     7141 gggtcaaccc gaaaaaaaag catcaaaaac gggagggctg gatttttcag ccaagtctgg
     7201 cactttgttt gatggctcct tcaacagtgc tcctccgtcg tgaactgctt gaagtacatg
     7261 gtatgtttga tgaaagactg cccgtttgtg aggattatga tctttggctc cggctctgtg
     7321 ctcagcatcc ggcggcctta ctgaacgaaa aattaatgac acgccacgga ggacatgcag
     7381 accagctttc acaaagagaa tggggaattg accgttaccg tgtacagtca attaataaaa
     7441 ttctgaaaac tgaaatactg aaaccggatg accgattagc cgctatccgg atgctccaga
     7501 aaaaatgccg tattttgatt cagggtttcc acaaacgcga taatatgaaa gaagtccgga
     7561 actatgaaaa aattatcagc caattttgaa ttcactaaat gagaacaaca tgacagaaat
     7621 taagacagat tttgaaatac gacaagccga agaagaggat gtgcctgaaa ttttagaatt
     7681 gatcaaagct ctggctgaat ttgagaatct ctctgatgaa gtggttgcaa cagaggagtt
     7741 gctgaagata acgctatttg gtataaattc tccggcagaa gtgcaaattg cttacgacaa
     7801 aaataaaacc ttgggctttg cattgtattt tcgcacgttt tctacttttt tgggacgtcc
     7861 aggaatttat ctggaggatc tgtatgtgcg ggaaagcgcc agaggaaaag gcgttggaga
     7921 agcattgctc cgccgattag ctcaacggac gcgggaaatt ggcggaggac gtttggaatg
     7981 gtcagttctt aactggaatg aagcggcaat taaattttat cagaaaatgg gagcatctcc
     8041 tttggatgaa tggactatgt tccgggtgac tggaaacaaa ttgaaggagt tggctgaaga
     8101 gtgattttga ataagtgatg tattttacca aatatctcca aaacgtaaaa gagttctaaa
     8161 ctgttaacgc ttttattttg attccggctc ttgcgttttt ttgtccggct ttatttttga
     8221 agattcttct ttttttgcta tggctttggt ttttctctct tctgttattt ggtcataaac
     8281 tctttgcaaa acacgatttg tcagaccatg tgaagtgtca tctacaaaat ctttgcatag
     8341 ttctacagct tcgtcaatga caacactatg ggatagttcc ggatggtgat aaagttcata
     8401 ggttgccaga cgtaatatat tccggacagt ggtggaaaga cggttcagtt tccagtgttc
     8461 caggttacgc cgcaaatatc gatcaaggtg tttgcgatgc tggagcgtcc ctgctaccag
     8521 ttcccgtgca aattctttta tttccggact ggtttcaagt tgatccaaaa aacgatcagc
     8581 atgtttcatg gctttacttt gaagcagttc cgcctgataa agggcctgta gtgcgtatga
     8641 tctagcttgg tggcgcatat ttctcaggtt gtcagtgcaa catgtaattt ttgaaccaga
     8701 gcctgattgt aaccatgtga tgtcatattg gcaacaactc ttcctcgcag tggaaatcca
     8761 agcagataaa ggtccccgat gagatcaagt attttgtgtc gtacaaactc atccggataa
     8821 cgtaagtctg tattgatgac ctttccatca tgcatgatta tatgagaatc aagatagccg
     8881 ctgccgacag ttcctttttt ttgcgccatg tcaatattct cgaaggtatt gaaagacctt
     8941 gctggtgcaa tatcactgtt gaaagaatcc ttttcagaat taaaggttag tttttgctct
     9001 ccaataggag cgtcataatc aacacgcatt ttaacctcaa agccttcata cggttccaca
     9061 tagaggtgtt tttcatccaa ttttttacgc cccacctgaa ttggctccaa aactacagca
     9121 tcttttgctg attcattctg atccagtatt ccggcttctt tgataagttc actaaaatcc
     9181 tttgctgaac cgtcaatgtt gggaatttct tcatcgactt tggctaaaac attggttatt
     9241 ccggccatat gtaaagcagc catcaaatgt tctactgtcc gtaccagacg gttatcacga
     9301 gctaatacag tcgagtttgc actaaatgat tgctttgcca cagattgaga aaaattttct
     9361 atatttgtga tatgtgcagg taaagatgta tcatccaatg tttgaaaaac aatcccggag
     9421 ttaggatcaa gtggacttaa aataacaccg gtgtttctac ctgtcaaaag ccctttacca
     9481 ttcaaaacaa cattgccttt caaggttcgc tggggacgtt ttgaatcctg taattgtact
     9541 gctccagaag gtattatggg attgataatc tttgctactt ccagcagagt atctgaggcc
     9601 agatctttga ttttttttgg aggtttattg gaaagaacaa ttgaaatttt gtcgagcaaa
     9661 atttcaaggc gcagtggttt ttccataaaa tcgacagcac ccagttttat tgcatttact
     9721 gctgcatcga tgcccgcgtg tccgctcatc atgattacgg gaatgtccgg atgagtttca
     9781 cgcaactgac tgagaattgc cattccatcc gttccaggga gccagatgtc caacagtacc
     9841 aaagccggag tttgtttctc cagttttgca taaaaatctt cagagtccct gcaggacatc
     9901 accggatagc cttcatcaga aagaacattt tcaagtgtct cccgaatcgc cggctcgtcg
     9961 tctactacgc atataatttg tgatttagcc atttagcaca ttatgtcaat gttttttctg
    10021 gtttaatcag ttcaaaattg atatttcaag cgttgaccta cttgaagttt tagttcgtcg
    10081 gcacgtccag cgtcaatttc cagcacaaaa tttccagcca caggaggtgt cattaccgga
    10141 ggaataactc cggactttgc cggctgaaca ttccggagaa tgtaagcaat tctgtgatta
    10201 tccagccaaa tgatatccaa tgggaactcc atatctttca tccaaaaacc atgctgttta
    10261 cgtttttcaa agacaaacaa catgccccag ccattttcca gaccagaacg tttccccagt
    10321 cccaagcttc gtttttcaac tgtgtctgcg acttcgacag aaattttctt tcctgtggaa
    10381 gttgtgacaa tcgcatgtgc ataatcctga acttcagcaa atgcaaagtt actaccgaaa
    10441 aaaggaacaa accaaaaata aataaaaata gtgcagaaaa aaaaatgacg caaaatcagg
    10501 cctcatcatt agcgagtttg gtcaaccgtt ttttaatcat tttctgttta agcagaggta
    10561 aatgatcaat gaataaaata ccattcaaat ggtcgatttc atgctgcaga caaatagaca
    10621 tcatatcttc ggcttccagt tttttagaat atccggcaac atcctgatat tcaatggtca
    10681 ttttttcagc acgatcaact tcggcccgga actcaatcac actcaagcaa ccttcttcag
    10741 agacagtggt tccagatttt tccagtattt caggattgat gaaaacatga ggttcccgaa
    10801 gttcggtgtc ttcctcacca ctttttaaat caataacaat tatttgtttc aggatacctg
    10861 cttgaatggc cgccaaacct attccgccgg aaaagtacat tgtttccaac attgattgtg
    10921 cggtttcatc aagctcttga tcaaaaactg ttaccggctc agccttttgc ctcaaaactg
    10981 aatctggaaa agtatgaatt tctaaaagtg ccattgctgt cttcaaactg aaaatacaca
    11041 gttaattatg gtaatatagc atttgttacc tcgattttaa ccaaactgca tctcaaacac
    11101 aatctgtgaa gcataaaaaa cgacacagaa catgctttgt cttgagaagg aaagtcagac
    11161 tgagctagaa ttttacatca ttcacttctc aaaaatctct tgccgtaaaa aatggaaccc
    11221 acatgaacac cggattttat acgaatccta tttaccttga acacgataca ggctcgcatc
    11281 cggaaaacgc taatcgtttg agggcgatac aagaaaaact tgagtcagag ggattgcttg
    11341 aaagattaat actgcagtcg ggacgttctg cgacatcaca ggaaataaaa ttattgcatt
    11401 cagagaaact gatttcagct gtcgaagctg cggctgaaag cggagccaga acactccaca
    11461 cgccggactg cattatttct gcacaaactt ttaatgcagc tgcgcatgct gtcggctcag
    11521 tgctggacgg agttattgag gtggctgaac gtcggcttga taacgcattt tgcgccgttc
    11581 gtccaccggg gcatcatgca gaaaatgatt ctgcaatggg tttttgtttt ttcaacaaca
    11641 ttgcccttgc agcagaattt ttgacaagag agatggggtt caaacgggtt ctgatttttg
    11701 attttgatgt tcatcatgga aacggaacac agcatttttt tgaagaacgg gccgatatat
    11761 tttttgccag tattcatcag gatccacgca caagttttcc gggaacaggt tttgcctatg
    11821 aacgcggaag tggcggaggt gccggattta ctcttaatgt tcccgttcct ccaggaatgg
    11881 gtgatgaaga atatttgcag atcttttatg atcaggttca accaaaattg gaggaataca
    11941 agccggattt tgtcttagtt tctgccggat ttgatgcgca ccgcgatgat cctttggctt
    12001 cacttaatct cacagagcga acttttcggg agttgacgag ggagttgaag cagttggcgg
    12061 aacaatatgc aggcggcagg attatgagtc tgctggaagg aggttatgat ttaaacgccc
    12121 tctcgtcatg cgttcaggaa cacctgttga ttttacatgc agaggattaa ttgaaccgaa
    12181 gtcttgttat ttttcagctt cttcttcaaa cattttttca gaattctttg gtatttgata
    12241 attgctgctg taaagaaaat atggaattcc gcccaacata aaaactccca gctggacaat
    12301 aatgaagaga ttgaaaatgt ctgctcctcc ggaaagtccg gccagttggt aaagtgtctc
    12361 aaaggccgca tgaccgattc cgattccacc cggggcaatt ggaatggcta cggtgattaa
    12421 gccaatcggc attagaaaaa attgggttgc caattccatt tctttaatgc ccatcaaatg
    12481 tgcaacctgg aaaaaaatca gtgcaattaa agtgtgaagg cctatggaca aaaataatgt
    12541 taagaataaa gttgtttttt gatgctgata acttttgaat gcggaataga ctttgatgct
    12601 gaatttgctt gcaggcaggc gctgaaaaag tcgaaggaaa ggatcacgac cctccttaaa
    12661 tggaaacaac acaattgaat aaaaaaggac cgttcctcca aatagagcaa caattatcca
    12721 tgccagactg tgaagtcgtt cttgagaaag tattaattca aaattaaaga ctagtgcaaa
    12781 aaatgccatg acaatcaaac cgaacaaacc cacaaaccgg tcaatcagca gtgtcataaa
    12841 cgctctggta cgtccatctt cctcttgtgc cttgatcaca taatatccct ttaccacgtc
    12901 tccagtcacc gctcctggaa gtgtggtgtt gaaaaaatta ccaatccagg tcagcacaaa
    12961 agtctgcttc ggatttactt gtattccaat cgcacgcaat aataaccacc agcgaaatgt
    13021 cgccataggc accactgcca aaatgagtat agcaaccatc atcgcgagta tttccggagc
    13081 atcccggaag agcatcaatt tttcaaaatt taagcggcca cttcggacta ggtaaatcaa
    13141 aatggttccg acgaccacaa ttttgagtaa tatcagcagc agctttttca ttttaaaatc
    13201 aaaaataatt aacgcaaaaa accaggatca ccaaaactcc tcctgcagat agcagtgtct
    13261 ttcgaacctg gttacaactc cagcagtatc ccagaatcaa caataatgaa aagctaatac
    13321 tgtaaaatag aggccagacc cagccctgta ataaccaggc agtaccggcg taccacagtc
    13381 cgtgcatggt ttctatccag atcgttctaa acccgattgt ggacttacat ttccggcaac
    13441 gtcccttact taaaataaat gacaataccg ggattttttc aaaccatttg agctgatgtc
    13501 cgcaggaaaa acaaaaagaa catgccggtt tgaataaatt cagggattga tcctggagtt
    13561 gctgtttcag gaaagtggaa atttcaggtg cattcaacaa actcaagcgt cttgttttgt
    13621 ctgcaaactg cagcggaagc cgatcaaggc aaacatttac aaaactgccg attatcagtc
    13681 ccagtaaagt ccaaaatcct atttcaaaat actgcactca tgctccagta ccggctgttt
    13741 ttagttgagc tttcaggttt ttcaaaactg ctgatgtata cgtaaaaaac tggcaaattt
    13801 tggcaggcct ttctttgtgg ttccagtgaa tttgtaagtt atgacagttc ctacagcagg
    13861 tggatttctt cgttctttat tactgaaacc tgtacctata cgaaatctgc ggccgctttc
    13921 atcagtcacc agtaatgaac ccatcattcc aatgaatttg ccttttccgg gtaaaatttc
    13981 tacaactgtg gcttctgcat cctgccacgg tttgagtttc agcaagtcgt cgcttcttcc
    14041 actgtgataa agtgaatcag cgcgatgcag catcaaccct tcaccaccct ttgttacaat
    14101 ttcatccagc atattcagca gagcatcctc tgaattcagg cgaatttgag gtataggctg
    14161 gagccaggaa atttttaggg tctctgtcaa tttcaccatt ttacggaccc tccgggtgaa
    14221 agttcctgga tgctccggaa gctcaaataa catgtaacga acctgtctcc agtcgttatg
    14281 atttggctga attttgcgga caattccgga aagcttttca aaagttcctc gtcccatcca
    14341 aagctcacca tccattaact gatcaggaaa attttctatg aaccagtctg gagcactaat
    14401 tagtttgccg ctgcgaaaat gaagtttttc accattccaa attgcgcgta ctccatccag
    14461 tttttcactg acccaccatt ctgatacatt ttcctgcttg tatacctttg ccagcaatgg
    14521 atcaatattt tgatctttat cattggtatc tcctgcagca agaggaaaaa caccaataat
    14581 aaaagtgaaa aaaactaaga tcagaagaag ttgggagtat aacggtttgt aaaacattac
    14641 tgattttgag caatatgttt gtcagtaaat caagtctaag ttcacctaca tgagaaaaat
    14701 caggatgtat agataaattt ttatcaggag ttagttttca ctagctaact tccagagatt
    14761 ttggcaacag gaagcgatcc agcaattcat tggcaagacg tatgtaacca gttgcaccgg
    14821 ctgaattcaa gtgacgctgt atcacatcta ttcctaaaga acttgcttcc agaatatgtg
    14881 ggcaatttgg aataatggtt tcaaaaacag aatcgcccag tgtttggtat acctgtttgc
    14941 atatttgctg gtgagtcaga atttcatcat tatacatagt cagtaaaatt ccagccaggc
    15001 gcaaccttgg ttcaattgtc ctctgaagtt ccttaaatgc ctgcaaaaag cgtttgagag
    15061 atttaatggc caatgcttca cattccagag gcacaatgac gaggttcgca gcaagcattg
    15121 cattaatgct gagggagctt gtagatgcgg gtggatcgac aattataaat tcatagctac
    15181 tggatgcatt ttgttccagc cattgtgaaa aatgataata atcagcagct attttgttta
    15241 cagtttgttc tgtcaggagg tcttgaatat tcgaaagcag gaggtcaaga ttctggtgtt
    15301 tgccggtttg gataatttcc tgtgacgaaa cacttggtgt gcaaaaaact tcacgtattc
    15361 ctaatccatc tggtttgtct aatccgagag aatggtgaac ggaaccttgt ggatcaaggt
    15421 cgaccaagag cgtcttataa ccgcccagtg caaaggcagt ggccaaatga acggctgatg
    15481 ttgttttacc tactccgcct ttttggctgg cgaatgtaat tacttttgcc attgtagaaa
    15541 ccgctcacct gaaaaagatt tagttttact gttttttaaa tcaatcacgt tgcaaaagcg
    15601 agtgtaacac aggatcagtg aaaagcaaac ttttctcatc caaacgtact acttaagctc
    15661 aaactgaatc aagtgcagtt tgtaattttt ctcgtacact ttttgcaaaa acatctaagt
    15721 cggttcttcc ggttgcctgc accatcattt ccggatcaat gactcctaaa acagtttctc
    15781 ctttttccgg atcttcataa acaactacgt tgcaaggcag caacaggccg atatctattt
    15841 cctcagataa aacttgaaaa gcaagtcctg gattgcaggc cccgaggatt acatattttg
    15901 taaaatcctg atcaatttta ttttttagtg ttgctttcac atcgatttca gtcaaaacac
    15961 caaaaccctg ttctttaagt gaagatttga ttttttcaac tgcttcttca taggaaaatg
    16021 gtacaacacg cttcataccg tattttaatg ctgatgacat ttttttctca ataagattaa
    16081 tatttggaag ttataactgc tggcgcaccc ggcaggagtc gaacctgcga ccctcagtac
    16141 cgcaaact
//
LOCUS       DTVD01000257            4453 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6938_length_4453_cov_330.829241, whole genome
            shotgun sequence.
ACCESSION   DTVD01000257 DTVD01000000
VERSION     DTVD01000257.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_4453_cov_330.829241"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            12..764
                     /locus_tag="EYO68_09655"
     CDS             12..764
                     /locus_tag="EYO68_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC08102.1"
                     /translation="MNQIDLHKRCAVVTGGAQGIGLAIAERFLASGASVCLWDRDESL
                     LKKTADALASKGTVKPVVMDVTDLNSVRNAAQKTEELLGGIDILVCNAGIAGPTAKTW
                     EYPPEEWQQVIDIDLTGVFNCLHVISPMMIAQNYGRIVNVASVAGKDGNPNAAPYSAA
                     KAGVIALTKSLGKELAGYDIAVNCITPAAAKTRIFDQISADQIQYMLSKIPRNRFLKV
                     EEAASMVAWLCSEENSFTTGGVFDLSGGRSTY"
     gene            963..2741
                     /locus_tag="EYO68_09660"
     CDS             963..2741
                     /locus_tag="EYO68_09660"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307304.1"
                     /note="catalyzes the formation of 3-methyl-2-oxobutanoate
                     from 2,3,-dihydroxy-3-methylbutanoate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIC08103.1"
                     /translation="MAAKKTSRLRSREWFDIPGDPSTTALHIERYTNFGISSEELRSG
                     KPIIGIAQTGSDLVPCNRIHMNLALRVREGIREAGGIALEFPVHPIQESGKRPTAALD
                     RNLQYLGLVEVLFGYPIDGVVLTIGCDKTTPALLMAASTVDIPSICLSSGPMMNGYWK
                     GERAGSGTIVWKARKLLATGEIDEEEFIQIVVDATTSPGFCNTMGTATTMNSLAEGLG
                     MSLPGCAAIPAPLSERIEMSYRTGLRIVEMVKKDLTPSKILTRKAFENSIVLNSAIGG
                     STNAPIHINAIARHVGVELEIEDWEKVGLEIPMLVNMQPGGKYLGEDFHRAGGVPAVM
                     NALLKAGKLHDSAITVNGKTVAENYAETTTQDTDVIRTFQNPMLENAGFIVLKGNIFD
                     SAVMKTSVISDSFRSQYLDKPGSKNVFEVRAIVFEGPEDYRKRINDPQLNINVDCILV
                     IRGCGPVGYPGSAEVVNMQPPDKLIREGILELPTLGDGRQSGTSGSPSILNASPESAV
                     GGGLALLKTGDMLRIDLNQGSLDVMLDKSELQKRHDELKTEEIENQTPWQEIYRNTVG
                     QLSSGGIIELAAKYRKIRNTIPRHSH"
     gene            complement(2794..3678)
                     /locus_tag="EYO68_09665"
     CDS             complement(2794..3678)
                     /locus_tag="EYO68_09665"
                     /inference="COORDINATES: protein motif:HMM:PF08450.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIC08104.1"
                     /translation="MTDFDLVGELECIWEVQAICGEGPLWIKDNQSLYFVDIDGQKLH
                     CYEETSNARRSLELEEKTGWVLPRCNHKDFVAGCESGVYFMDKSSGKMTFVMNPEPDQ
                     VENRFNDGKCDREGRIWAGRSHDPETSATGYLYRIDPDLSHSRWDGPYICPNGPAISP
                     DDNTLYHVDTFGGTVWAFDKHPDGTISNRREFARLDSKKEGFPDGLTVDNENRVWLAH
                     WGGSRITCFSPQGERLGFVELPVPQVTSCTFGGPDLSALFITTAARNLDLVKYPLAGA
                     LFRVPTKTTGSSSPAFAG"
     gene            complement(3793..>4453)
                     /locus_tag="EYO68_09670"
     CDS             complement(3793..>4453)
                     /locus_tag="EYO68_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIC08105.1"
                     /translation="QNALYLAFNFIAMQWIEAGLAAIIASLLPLIVAVVCWLFLGEKT
                     GFTGILGLVAGFGGVLVIMLDKLSGSSASLGMALCLIGLAGLAAATLYVGRMMSRNKN
                     VLMIVGLQMIVGCIILFPFSLIFENWNIEWSTSFILAFLYTTLVPGLLGTLIWFYLVR
                     RVGPVRAATFHFLNPFFGVLVAALILSEPLSVRDGIGVTIIMAAILLVQMSRKQIANA
                     D"
BASE COUNT         1266 a         1083 c         1039 g         1065 t
ORIGIN      
        1 ggagcgtaac tatgaatcaa attgatctgc acaagcgctg tgccgttgta acaggaggag
       61 cccagggaat aggtctggcg attgccgaac gatttcttgc ttcaggcgcg tcagtctgtc
      121 tttgggaccg cgacgaatca ctgctcaaga aaactgcgga tgctcttgca tcaaagggta
      181 cagtgaaacc tgtggtaatg gatgttactg acttgaactc tgtcaggaat gccgctcaaa
      241 aaactgaaga attattgggc ggcatagata tcctggtctg taatgcagga attgccggac
      301 ctacagcaaa aacatgggag tatcctcctg aggaatggca gcaagtcatt gacattgatt
      361 taacaggagt tttcaactgt ctgcatgtca tttctcctat gatgattgca caaaactatg
      421 gacgaatagt taatgtggct tctgtggccg gaaaggatgg aaatccgaat gccgcgccct
      481 acagcgccgc aaaagccgga gtgatcgcac tgaccaaatc tctggggaag gaactcgccg
      541 gttatgatat agcggtcaac tgcataacac cagctgcggc caaaacacgc atatttgatc
      601 aaatcagtgc tgatcaaatt cagtatatgc tcagcaaaat accccgcaac cgttttttaa
      661 aggtggaaga agcagcttct atggtagcct ggctctgttc tgaagagaat tcttttacaa
      721 caggaggtgt tttcgacctg agtggtggcc gctctacata ctgatttgac cgcttatgta
      781 ggcggatata aagtgtcagg acattatctt agtattttct taatctttta gacatattgt
      841 agggaatgtt tagattattc tgtttcggta ataccttaat tactgccaaa aaatggtttt
      901 cctgataaaa tcaaagatct aagatcattc ctgacaggct taactgcaca agttaaagaa
      961 taatggcagc aaaaaaaaca tcaagattga gaagccggga gtggttcgat atcccgggtg
     1021 atccaagcac tacggccctg cacatcgaac gctacaccaa tttcggaatt agttccgaag
     1081 aactccgctc aggaaaaccg atcatcggta tcgcgcagac cgggagcgac ctggttccct
     1141 gcaaccgcat ccacatgaat ctcgccctca gggtccgtga aggcattcgt gaagcaggcg
     1201 gtattgctct tgagttcccg gttcatccta tccaggaaag cggtaaacgt cccactgctg
     1261 ccttagaccg caatcttcaa taccttggcc tggttgaggt actcttcggc tatcctatcg
     1321 acggtgtggt gcttactatc ggctgtgaca aaacaacacc agcattgctg atggcagcat
     1381 cgacggttga tatcccctca atctgccttt catcaggtcc aatgatgaac ggatactgga
     1441 agggtgaaag agccggatcc ggtacaatag tctggaaagc cagaaaatta ctggcaaccg
     1501 gtgagattga cgaagaagaa ttcattcaga ttgtcgtaga cgccactacc tctccgggtt
     1561 tctgcaacac aatgggtacg gcgaccacta tgaattcact ggccgaaggg ctgggtatgt
     1621 cattaccggg atgcgcggcc attccagctc cgctcagcga acgtattgaa atgtcctaca
     1681 ggactggtct gcgcattgta gagatggtaa aaaaagacct gacaccgtct aaaatactga
     1741 caagaaaggc ttttgaaaat tcaattgttc tgaattcagc tatcggcggt tccaccaacg
     1801 ctccaatcca tatcaatgcc atagcccggc acgtcggtgt agaactggag atagaggact
     1861 gggagaaggt tgggcttgaa ataccgatgc tggtcaatat gcagcccgga ggcaagtacc
     1921 ttggtgagga ttttcaccgt gccggcggag ttccggcagt aatgaatgcc ctgcttaagg
     1981 caggcaaact ccacgattct gccatcacag tcaatggaaa aactgtggct gagaattatg
     2041 ccgaaacaac cactcaggat acagatgtga tccggacttt ccaaaacccg atgcttgaaa
     2101 atgcaggttt cattgtcctc aaaggaaata ttttcgattc tgcggtgatg aaaacatctg
     2161 taatcagcga cagttttcgt tctcagtacc ttgacaaacc aggcagcaaa aacgtctttg
     2221 aggtccgggc catcgttttt gagggtccgg aagactatcg caagcggata aacgatcctc
     2281 agctaaacat taacgtagac tgtattcttg tgatccgcgg gtgcggtcct gtaggctatc
     2341 caggatcagc agaggtggta aacatgcaac cgccggacaa actgatccgt gaaggaattc
     2401 ttgaactgcc gactctcggt gacggacgtc aaagcggtac atccggcagt ccttctatcc
     2461 tcaatgcttc gcctgagtca gcagtaggag gaggattggc cctactgaaa accggtgaca
     2521 tgctgcggat tgacctgaat caaggttcat tagatgttat gcttgacaaa tctgagctcc
     2581 agaaacgtca cgatgaactg aaaactgagg agattgaaaa ccagactccc tggcaggaaa
     2641 tataccgtaa cactgttgga cagctttcca gcggcgggat catagaactg gcagccaagt
     2701 acagaaaaat caggaacacc atacctcgtc actcccactg aagtcgtatt caaaactagc
     2761 ttccacaaaa atgcgaagcg tgtctatctt cgactatccg gcaaaggcag gtgaggaact
     2821 ccccgtagtt ttcgtaggta cccggaaaag ggcaccggcc agcggatact taacaaggtc
     2881 caggttgcga gctgcagttg taatgaagag ggcgcttaaa tcgggacctc caaaagtaca
     2941 gctggtaact tgcggaacgg gaagttcaac gaaaccaaga cgttctcctt gtgggctgaa
     3001 acaggtgata cgcgagcctc cccagtgtgc aagccagacc cggttttcat tgtcaactgt
     3061 gagaccatcc ggaaatcctt ctttttttga atccagtctt gcaaattccc tgcggttgga
     3121 tattgtaccg tccggatgct tgtcaaaagc ccaaactgtt cctccgaaag tgtcaacatg
     3181 gtaaagggtg ttgtcatccg gactgattgc aggaccattg gggcaaatat atggtccgtc
     3241 ccacctggag tggctgaggt caggatcaat ccgataaagg tagcccgtcg cacttgtttc
     3301 agggtcatgt gaacgccccg cccaaattct gccctcccgg tcgcatttac cgtcgttgaa
     3361 acgattttct acttgatctg gttcaggatt catcacaaat gtcatctttc cggaactctt
     3421 atccatgaaa taaaccccgg actcacaacc ggcaacaaaa tctttatggt tgcaccgagg
     3481 taatacccaa ccagtttttt cctcaagttc caaactgcgc cgagcgtttg atgtttcctc
     3541 gtaacagtga agtttctggc catcaatatc cacgaagtaa agagactgat tgtctttgat
     3601 ccacaaagga ccttccccgc aaatcgcttg tacttcccag atacattcca attcccccac
     3661 caggtcaaag tcagtcataa tctactttac aaatattcta gaaagtcact gccaacagcc
     3721 aagccggcaa taccagaaaa ttctcacaca cacatattgt cggaacattg tcgcatcaac
     3781 accattgcac agttaatccg cgtttgctat ctgctttcga ctcatctgca ctaacaggat
     3841 cgctgccatg atgatggtta caccaatgcc gtcccttacg gacaacggtt ccgatagaat
     3901 aagtgcggca accagtaccc caaaaaatgg atttaggaaa tggaaggttg cagctctaac
     3961 gggtcctact cttctcacca gataaaacca gataagtgtt cccagcagtc ctgggacaag
     4021 agttgtatat aaaaaagcaa gtataaaact tgtactccat tcaatgttcc aattctcaaa
     4081 aatcagtgag aaaggaaaca gtatgataca gccaacaatc atctgaagcc cgacaatcat
     4141 gagcacattt ttattgcgac tcatcatccg gccgacatac aaagtagctg cagccagtcc
     4201 ggcaagacct atcaggcaaa gtgccatacc caaggaagcg ctgctaccgc tcaacttgtc
     4261 cagcatgatg accagcacac caccaaaacc agctactaga cccagtattc cagtgaaccc
     4321 ggttttttct ccaagaaata accagcagac gacagcaact attaagggaa gcagactggc
     4381 aatgatggct gccaaaccgg cttcaatcca ctgcatcgca atgaaattaa aggcgaggta
     4441 gagggcattc tgg
//
LOCUS       DTVD01000258            4433 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_6996_length_4433_cov_409.253997, whole genome
            shotgun sequence.
ACCESSION   DTVD01000258 DTVD01000000
VERSION     DTVD01000258.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6996_length_4433_cov_409.253997"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="EYO68_09675"
     CDS             complement(<1..1805)
                     /locus_tag="EYO68_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIC08106.1"
                     /translation="MENKDSKIRMISSGAKRLLLFLWPFAKKEKPVYNLKSIRIPRTS
                     GRALKILLFLLRTPGVRLLLLPLLLRQVGLPRLRKCHVEDAPVMHPVHPADSKITLAA
                     AKKSIKQFIVSAEQNRESATDKKGANSEKQSLFQPETASDFHRAYLEGKTTPVDVAMR
                     LLDIIRSIEGSAVPLLPFMAWEERELMQQAKSSAERYERGESLGILDGVPVAVKDELD
                     MLPFPTMVGTKFYNTQPPAEDATTVERLRSAGALMVGKTNMHEIGMGVTGLNTHYGTT
                     RNPYHLDHYPGGSSSGSAAAVAAGLCPIALGLDGGGSIRIPASLCGVVGLKTTWGRIS
                     SAGSAPLSWSLSTVGPITSTVRDTALAYSFLAGPDPRQVWTQDQPSVDLQDFDDSDLT
                     GVRLGVFLPWFNHASVDIVQSCNQMLDSFKSLGATVQEIDIPDLDEIRMAHAVSFLTE
                     LLTSLKTNFNPFRQPLGLDALVNLTLAQQFKGDDYVKAQRVRAQLIRKMSQIFQNVDA
                     VVTPSTARTAPPIPPDALKKGESDLSTLTELMRYAPQANLGGFPAISFPVGYDAQGLP
                     VGMQLMGRPWEESLLLRLANTSERFFARHPPMMRFS"
     gene            complement(1827..2951)
                     /locus_tag="EYO68_09680"
     CDS             complement(1827..2951)
                     /locus_tag="EYO68_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIC08107.1"
                     /translation="MPASDRHRATRRILIVDDIEDNCLLIEDHLQRQLKCEFRKASSG
                     NEALQILKNWSPDCILMDIMMPVMDGIEATKRIKMIPACHDIPVLMVTAKQEMDSLEE
                     AFDAGAVDYIYKPIDRTILVSRVKSALRSKEDLDRIKELNKELQQKKQELSSFSHTVS
                     HDLKSPIVGAASLYDFFLYRIKNEFPQIIEDEGLKELLERIPQTFTKMLDFINTQLDY
                     AEAGQVIGELESVSMDRIINAVIENFEYAEQEGIACFSKLPDMPEARCDPIRMLKVWQ
                     NMIANSIKFRGTKNPVRIKFGGEESRDTVKFWIRDNGPGIIAEHQDKVFETNARLNYD
                     VEGYGFGLATVKKIIAAHGGNIWIDSEVKDGACFVFELPR"
     gene            complement(2966..4369)
                     /gene="lpdA"
                     /locus_tag="EYO68_09685"
     CDS             complement(2966..4369)
                     /gene="lpdA"
                     /locus_tag="EYO68_09685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIC08108.1"
                     /translation="MTKFDTTIIGSGPGGYVAAIRCAQLGMQTAIIERYPTLGGTCLN
                     VGCIPSKALLDSSEHFYNANCHFEAHGIMAEKIKLNWPRMRQRKDDVVGQTCDGIKYL
                     MKKNKIKVISGHASFSDSKTISVSGTDGSKETVNTERVIIATGSKPSSIPGVIIDKKR
                     IITSTEALNLEKVPEKLIVIGGGVIGLELGSVFSRLGSKVQVVEFLDRLIPGMDFSLA
                     KEVQRFLKKQGLEFFLNHTVQSTVVEGDSVKVVAKNKKDKEVEFSGDYCLVAVGRRPF
                     TDNLGLEKIALTLDDKGRIPVNEHLETAVPGIYAIGDVVAGDMLAHKAEEEGVFVAET
                     IAGQKPHINYKAIPGVLYTWPEAAGVGFTEDELKAENYNYKTGNFPFKALGRARASMD
                     TEGFAKVLADKDTDEILGIHLVGARAADLIAEAVVALEYRASAEDIARMSHAHPTYAE
                     AIKEAMLDASEKRSIHM"
BASE COUNT         1168 a         1074 c          881 g         1310 t
ORIGIN      
        1 gagaaacgca tcattggcgg gtgtcgtgca aaaaaacgtt ctgaagtatt ggccagtcta
       61 agcaacaaag attcttccca cggacgcccc attaactgca tccccaccgg taaaccctgt
      121 gcatcgtaac cgacaggaaa agaaattgcg gggaatcctc caagatttgc ctgcggtgca
      181 tagcgcatta attccgtcaa agtactgaga tccgattctc cttttttcaa agcatccggt
      241 ggtatcggag gagcggtacg ggccgttgat ggagtaacaa ctgcgtcaac gttttgaaag
      301 atttgactca tcttcctgat aagctgggcg cgtacacgct gggcttttac ataatcatct
      361 cctttaaact gctgtgccag agtaagatta actaaagcat ccagtcccaa gggttgacga
      421 aaagggttaa aattagtttt cagagaagtc agtagttctg taagaaaaga caccgcatgt
      481 gccatacgta tttcatccag atccggaatg tcaatttcct gtacggtagc acctagagat
      541 ttaaaagaat ccagcatttg attgcagctt tgtacaatat ctacggacgc atgattaaac
      601 cacggtaaaa atactcccag tctaactcct gttaaatctg aatcatcaaa atcctgaaga
      661 tccaccgatg gctgatcctg tgtccaaacc tgtctgggat caggacctgc cagaaacgaa
      721 taggctaaag ccgtgtcacg tactgtggag gtaattggac ctactgtcga gagactccat
      781 gatagaggtg cactgccggc agaactgatc cggccccaag ttgttttaag tcctacaaca
      841 ccgcacaaac ttgcaggaat cctgatagaa ccaccaccgt caagtccaag tgcaattgga
      901 cacaaccctg ctgccactgc tgctgctgat ccgcttgaag atcctcccgg atagtggtca
      961 aggtgataag gatttctagt agtaccatag tgagtattga gccccgttac ccccattccg
     1021 atttcatgca tattggtttt acccaccatc agtgcaccgg ccgaacgcaa tctctcaacc
     1081 gttgtagcat cctccgccgg aggctgagtg ttgtaaaatt tagtaccaac cattgttgga
     1141 aaagggagca tatccagttc atcttttact gctaccggaa ctccatcaag tattcccaat
     1201 gattctccgc gttcataacg ctctgccgat gattttgcct gctgcatcag ttcccgttct
     1261 tcccaggcca taaaagggag cagtggtaca gcacttcctt caatactgcg aattatatcc
     1321 agtaaacgca ttgcaacatc taccggagtt gtttttcctt caagatatgc ccgatgaaaa
     1381 tcagaggccg tttcaggttg aaaaagactt tgcttttcag aattagcacc ttttttgtca
     1441 gttgcagatt cacgattttg ttcggcagaa acaataaact gtttgatgga ctttttggct
     1501 gcagccaaag tgattttact atctgcagga tgaacgggat gcatcacagg tgcatcttca
     1561 acatggcatt ttcgtagtcg gggaagaccg acttgacgaa gtagcagcgg gagcagtagt
     1621 aaccgaactc ctggggttct caacaggaac agcaatattt taagggcacg tccggatgtc
     1681 ctgggaatgc ggatggattt aaggttataa acaggttttt ctttttttgc aaacggccac
     1741 aaaaagagca aaagccgttt tgctccggat gaaatcattc tgattttact atccttattt
     1801 tccatctcaa actatattta ctttgattac cggggaagtt caaaaacaaa gcacgctcca
     1861 tctttgacct cagaatcgat ccaaatattt ccgccatgtg cagcaataat ttttttcaca
     1921 gtagccagcc caaaaccata tccctctaca tcgtaattca agcgggcatt tgtttcaaac
     1981 actttgtcct gatgttccgc aattattccc ggcccgttat ctcgaatcca aaatttaaca
     2041 gtatccctac tttcttcacc accgaatttt atcctgacgg gattttttgt accccgaaat
     2101 tttatcgaat tggcaatcat attttgccag actttcagca tccggattgg atcacacctt
     2161 gcttccggca tgtccggcag cttgctgaaa caggctattc cttcttgttc tgcatattca
     2221 aaattttcaa tcaccgcatt tattatacga tccatcgata cagattccaa ttcaccaata
     2281 acctggcctg cttccgcata atccagttgc gtattgataa aatccagcat tttggtaaaa
     2341 gtctgtggaa tccgttctag taattctttt aagccttcat cctcaatgat ttgtggaaat
     2401 tcatttttga tgcggtacag aaaaaaatca taaaggctgg ctgcaccaac gattggagat
     2461 ttaagatcat gactgactgt gtgtgagaaa ctgctaagtt cctgtttttt ttgctgcagt
     2521 tccttgttca gttctttgat tctgtcaaga tcttctttgc tgcgcaaagc tgattttaca
     2581 cgggatacta aaatggtgcg atcaatcggt ttgtaaatat aatccactgc tcctgcgtca
     2641 aaagcctcct caagggaatc catttcctgt ttggccgtta ccattaatac aggaatatcg
     2701 tggcatgcag gaatcatttt aatacgtttc gtagcctcaa ttccatccat cacgggcatc
     2761 ataatatcca tcaaaatgca atccggactc cagtttttca gaatttgcag ggcctcattt
     2821 ccagacgaag cttttctaaa ttcacacttc agttgcctct gcagatggtc ttcaatcagt
     2881 aaacagttgt cctcaatatc atcaacaatc aatattctgc gggtggccct gtgccggtca
     2941 ctggctggca tgaaattttt ttcagttaca tgtgtataga gcgtttttct gatgcgtcca
     3001 acattgcttc tttgatggcc tctgcataag tcggatgggc atgtgacatc cgcgcgatat
     3061 cttctgcgct ggcccggtat tccagagcaa caactgcttc tgcaatcaaa tctgcagcac
     3121 gtgctccaac caaatgaatt cctaatattt cgtcagtatc cttgtctgcc aggacttttg
     3181 caaaaccttc tgtgtccatg gatgcacggg cacgtcctag tgccttgaac gggaaattac
     3241 ccgttttata gttataattt tcagctttta attcgtcctc agtaaaaccg actcctgctg
     3301 cttccggcca ggtgtagagc actcccggaa ttgctttgta attgatatgc ggtttttgac
     3361 ctgctatcgt ttccgctaca aaaactcctt cttcttctgc cttgtgagcc agcatatctc
     3421 ctgccactac atcgccaatg gcatagattc ccggaaccgc agtttccaaa tgttcattta
     3481 ccggaatacg acctttatca tcaagagtaa gggcgatctt ttccaaaccc aaattgtctg
     3541 taaagggcct gcgtccaact gcaaccagac aataatctcc gctgaattcc acttctttgt
     3601 ccttcttgtt ttttgcaaca actttaacac tgtctccttc tacaactgta ctttgcacag
     3661 tatggttgag aaaaaattcc agaccctgtt tttttaaaaa ccgttgaact tctttagcca
     3721 gtgaaaaatc cattccggga attaaacgat caagaaattc cacgacctgc acctttgaac
     3781 caagtctgct gaaaactgaa cccagttcca acccaatcac accacctcca atcacaatta
     3841 atttctccgg aaccttttcc agattcaggg cttcggttga agttatgatg cgttttttgt
     3901 caattatcac acccggaata cttgacggct tggaacctgt tgcaatgata acgcgttcgg
     3961 tgttcacagt ttccttggaa ccgtcggtgc cactcactga aatcgtcttt gaatcgctga
     4021 atgatgcatg tccggagatg accttgattt tatttttttt catcaaatac ttgattccgt
     4081 cgcatgtctg ccctaccaca tcgtccttac gctgacgcat ccttggccag tttagtttta
     4141 ttttttccgc catgataccg tgtgcctcaa aatgacagtt tgcattgtag aaatgctcac
     4201 ttgaatccag caaagccttt gaaggaatgc aacccacatt caggcacgtt ccacccagtg
     4261 taggataacg ttcaatgatt gccgtttgca tccccagttg tgcacagcgg attgcggcca
     4321 cataacctcc tggccctgaa ccgatgattg ttgtgtcgaa tttggtcatt gtttgttata
     4381 ttgaaagtaa ggtgaaaatg gtactaaggc tctttttgaa ttaggcacta agg
//
LOCUS       DTVD01000259           16056 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_703_length_16056_cov_236.940004, whole genome
            shotgun sequence.
ACCESSION   DTVD01000259 DTVD01000000
VERSION     DTVD01000259.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16056)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_16056_cov_236.940004"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(30..1073)
                     /locus_tag="EYO68_09690"
     CDS             complement(30..1073)
                     /locus_tag="EYO68_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08109.1"
                     /translation="MIKVNGFAQMVTEDADWAEIEVPYYLKTGKNLFTIFVQTETGQS
                     EQEFIVTYEPQKKERKKPPPLNGVVMFGQTNSDNILSAQEGKSKTSASKNDLLLSAAY
                     AFELNEESAVSINAVLKFDRHQNRSLAAEEVLFRQFSTEYRHKNLLGLDLKTGLGQSV
                     ISVKDANPPDPKKAGEFRQDLQSLFLFVDSKKHWGSKFSGSLKMQIDSQNKVKTNSED
                     GSLTLASLGAKMRWEDFRFQARIDSQSTQFEDASKDYQSTLINVGTIYSWTPWVFGFN
                     FQNNNKQYQNADPASNLVLQNKKDEMTLNFKYSFSSSTLAGADLKQIKQASNDETRSY
                     KANQITVQYIWMF"
     gene            complement(1289..2860)
                     /locus_tag="EYO68_09695"
     CDS             complement(1289..2860)
                     /locus_tag="EYO68_09695"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08110.1"
                     /translation="MFGEKKTRSKEAKWMVTFADLITLLFCFFVYLSLFNKPQVDLKT
                     GFIVSEKTISNLTERLPENIVKGFKSMKGTYFDTKEMFTEKLEILLGQKQTGLYKTQI
                     LIESMATGKVEESAGVMKVEILLNERVEEDLRIPLFFAGNARRGPVDPELCTMEGLMK
                     NPKEIQEFDYVLGAELAIIPGGEFGASFPLCLVNDELYEEPEEILVQIGKLRGDVERG
                     NFVTRSIIIQDDEPLPTVTFEIARRDLYKGISNITANISPISGVKTDIPLKFAGTAKE
                     RKDFRFMDGATIEIYPYTEKGTVEIEVIQDEVPLYATRTLIIEMEDNSVLNADVGKIS
                     KQVNTIIGAQEMKDCSGINRFLRENEAFASFELNASKSRCILSLPSSFLFLSGGASIS
                     KEVVVQLSSFLNEIRNRYELEGDAIRVDGHTDDVPISKKGKYKNNWELSTVRATNVAT
                     LMMEKVGFNPERIAISGYADTRPKASYVSENGNRKSGRELQKARKANRRVELIFTRPT
                     KKERTRKFFPEPNAG"
     gene            complement(2948..3814)
                     /locus_tag="EYO68_09700"
     CDS             complement(2948..3814)
                     /locus_tag="EYO68_09700"
                     /inference="COORDINATES: protein motif:HMM:PF01618.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08111.1"
                     /translation="MKQQSADKLGTLFGFIGGTTFILSGILWPSEFSNLALWLESTGD
                     ADSYNKYVIQILRFFDLKSLFIVFGGTISVTFVAFPYKKALRSFGSIPKVFAADVAES
                     ATQEIYDKTKIIAEKRYSGKRITNDDLSSLENPFMRRWIEGLIVREQVEEESLAQILK
                     AEVLMYEQRADEEIEIFDFMGTAAPAFGMVGTIVGLVLMLAETGSIRSVMQAMSVAML
                     TTLYGVIFSNMVLLPIASKRKQLKESNLVLLEMIRESVNRIARREAPYTILQELHNFL
                     PHKRDEFDDQYG"
     gene            complement(3811..5436)
                     /locus_tag="EYO68_09705"
     CDS             complement(3811..5436)
                     /locus_tag="EYO68_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HIC08112.1"
                     /translation="MKIFRILNKKLLSFAGCIVVLTIFNFDILHAEILSRSRPEPEIR
                     ERIHKILRKTLREEYIDVSVILHSVLDNEPIKKDSDLLPGVKIKTEENDVQALFSYRI
                     INLTVNKSVNLNQVEMRIRSEISELNSQDRFEQSIVLDKLQIERPDFSEKLVEYVNLL
                     VGARADLNGDEPVNAQEKLKQAAEISATFPAGYEMLGVDQLIPEYKPEGTDAITPAQW
                     AIIGTLGVLLLLTLALFFFVSRSTREKEEKEHPLQGTLGKVATAIEAMAPEEEAEEEE
                     LDLPDEEADAGDDEEKIDYFGFIRKMPPKLRVDLLQEMKPHQQAIVFSQMDPSEMANI
                     LKGLPVEDSLGIVLTMKDISVSPSELQQLADELTESAKNLPMPFDGLGRIGELSGQIS
                     AEEWNDILGSTDELKKKNPELAKNTSEAMELIRERSLSFEDLESTEIIDDAFVGRVME
                     RFERSRLSTGAKVSVPYLLLDSHSSVTEKIMKEYTEETRKYYQEQLQGYEKQREEDEE
                     AKLRIEQLRNLHRNVFMRHVHIELGKIWEKKDS"
     gene            6374..7330
                     /locus_tag="EYO68_09710"
     CDS             6374..7330
                     /locus_tag="EYO68_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HIC08113.1"
                     /translation="MLKYFSILFTLLTLVLAGCTFSDPDPEEIQAQMDQEHARITEEQ
                     ERQQEEEAEKLLAQETAAAEETAAKEAEAARLAAVDKETALDKIEKLLKPASIEVIQP
                     EEPKSEPSIINTISSGLKNLLGSSVKEQQEEPKKAADNVSASEKKSTTKFMPKSPLAI
                     FSAQKLVSCKIVPEKMEAIHKRLLKIKDVNIADSKLRDKTNDKLMPVIHFDFDQIKIK
                     PAYRKLLRQQSSCVMKALKSRNDMIIQIEGHADERGSDEYNMALGHRRANVVANAIKA
                     YLPDSSLARIFSFGEEFPLVRLSNKNAWAKNRRVEFTLLLKP"
     gene            7339..8019
                     /locus_tag="EYO68_09715"
     CDS             7339..8019
                     /locus_tag="EYO68_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HIC08114.1"
                     /translation="MDFLTLPFTTDFSSIAFSVLAILLGCSVIVWITLFMKFFSLIRV
                     KHLQARALKLLENTQRVSELRLRFSNMPKNPLQEMFALGDRELKIFLKTSPESDYGHR
                     RELMDRLERILEARILLAEKDLNAGQALLATIAATSPFIGLFGTVIGVINTFMSIAEF
                     NSVELSVISPGIAEALVATAAGLFAAIPATLGYNLFRATIRGMTETMDYFALQLLHHL
                     QKRLLPKK"
     gene            8058..8438
                     /locus_tag="EYO68_09720"
     CDS             8058..8438
                     /locus_tag="EYO68_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIC08115.1"
                     /translation="MSEINITPFVDVLLVLLISFLISAPLLTQAIQIQLPKGKLKERV
                     PVYKESILAVVNKKKQIVISDQIYSLKSLAKSLTKNANEWKRNQPVYVQMDEQVPYGI
                     LMDLMLLFKNAGFPQVGLVFKKES"
     gene            8491..9633
                     /locus_tag="EYO68_09725"
     CDS             8491..9633
                     /locus_tag="EYO68_09725"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIC08116.1"
                     /translation="MKLMMVAGEVSGDAHGAGLIRSLRKNHDSADIFGIGGKQMLREG
                     FRPYFMLDSLQTHGLVEVVQHLPRLYQTLWRMRQALTEEKPDALILIDYPGFNLKLAA
                     YAKKTGVPVIFFNSPQIWAWRKGRLNTIKRVVDKMIVLFPFEEKIYQQAGVDVKWVGH
                     PLLDEMQSEIDSGAIRKQFGLQNNILLIAIAPGSRPSEMRRHLPTLLEALPQIQQKTG
                     EIQCILPVAESLDLKEIRKLTDTSSVPVTLVPEQFIETIQACDAAIVASGTASLQTAL
                     ALKPFVIVYQVSPLTYRIARYLSETEYVGLVNVLAEKEIVPELLQNNFTVSKVTEIAV
                     RLLQDEDYRATMISQLKSTRTKLGEAGAYDKAARCIENVLSREPAI"
     gene            9663..11162
                     /gene="tldD"
                     /locus_tag="EYO68_09730"
     CDS             9663..11162
                     /gene="tldD"
                     /locus_tag="EYO68_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509820.1"
                     /note="responsible for the proteolytic maturation of the
                     E. coli pMccB17 plasmid-encoded microcin B17, an exported
                     protein that targets the essential topoisomerase II DNA
                     gyrase; degrades the E. coli plasmid F-encoded CcdA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIC08117.1"
                     /translation="MKTAELSLDTHNIAHHLTQTLTGVEAVKQLFEGQMGIDKTVVRQ
                     LLSAALHRGGDFADIHFEYSTRNSVVMEDGIIKNSAVAVVSGVGIRVVQGDQTGYAYS
                     EDFDLKPMLHAARTAAAIASSGEVSLSESFRFNELVPRDYYPVLETVTDLELVQKIDM
                     VKRTEAVSRAYDERIIRVTVAMMDALNLTQVVTSEGSILRDTRPMFRMNVHCVSQEAD
                     NIQNGSSGIGGRVGLDFLKQKDHAVNLGKQAASEAILLLGAKQAPSGLMPVILGPAQS
                     GILLHEAVGHPLEADFNRKGTSAYSGRIGEKVASDLCSIYDSGTIPHERGAINFDDEG
                     VPSAENILIENGVLRNYMHDRISARHFRTNPTGNGRRESYAHYPIPRMTSTYLANGES
                     DPEEIISSVKKGVYCQEFSGGQVDISNGDFVFVPTVAWLVEDGKKTVPIKNFTLIGNG
                     PDAMSKVSMVGNDFAMSEGIWTCGKEGQSVPVGVGLPTVLISEMTVGGM"
     gene            complement(11360..12916)
                     /locus_tag="EYO68_09735"
     CDS             complement(11360..12916)
                     /locus_tag="EYO68_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08118.1"
                     /translation="MADQAQDKRDQEGNGNKEKLILLLLRKDLRKIYMKTLVDRLHDS
                     GVTVAVDETNYPEEKQKKFHWILSFPGDIPKGKAHDASSLGLIFRVPGMPKLQMEKLP
                     SSDSVKLTKLIGRYKEVQQTCIDREKHPAFVKLALIDAVLRGKQQLIQKFEDLENRLE
                     QKISQVEKPYYKGIGYILSTSIEKITGIAVLDGLMKGLVHVMIIDDMEKRTIDGLVAL
                     DFKRKFLSFMSTSELSKKIQEFRKAHAEDEFAKANIEEFIFNSPDFEHIEIVFFNSWK
                     FTEDSFPVRVALRKKAIISKKEERKVQQKDQAEGKIAAEEKALVVVKEKLEEVMRVIN
                     KMEKSHFDDEDEYQLQTSARKRFLKDIKIRELRLKELKRPISKGAGEEKIITIDLFDI
                     FKNNQSFMDRMSDKAGSLGLGELWGKHVKTDDSFSFNKLAEMARFSKDWIKASAQLKK
                     IKSQTTHLAKQLKLFVEKNELTNSVSSSGSGTEGNYQPLLDKHILDRLELAVLSCEIS
                     NLTADKLKKE"
     gene            complement(12924..14483)
                     /locus_tag="EYO68_09740"
     CDS             complement(12924..14483)
                     /locus_tag="EYO68_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HIC08119.1"
                     /translation="MQLPNLDEMSAEEKMWFANSIAGMVVADGHADQSEMSFLREAIN
                     FMDDKDEIDKLMVIIKNGNPPELGPLDIDPKQAFLMLKYLAQLMVADADLSPKEISYF
                     LLAGRSLSFNNEILTKLWKSARALLERDLPQAIVETGSLKTKVSLTKVDETGVTFRLG
                     KALMPKVKIMLYVLKSVHSELPLKGNEEHWDPLDCKMEKQHQVKFDEGSYVVRAHIEQ
                     RLFEDHGIMQIMHPEDYAVVSDGGFFDTEKDSLLGSFLDCYVCDNPKIKFYVLHSKSM
                     ITDPNIFGVSSFVRSAGELKFCDFNLIQVASCSKCGFSSNDKEHFKRLKTSEPTFSVE
                     EFSKGWEEKIEPLLKKAQEIGETFYGEERDIQQGILSYDLAIATFEQMASIASNDNVK
                     GAALRKKASMLMIQAEMLMESKNRDAAEANLKKTVDTLEPIFESLEGLHLLHTCVLLF
                     QIKIYLNELQSAAQYMKFLDNYDTDGKLKEGTEEFKELKVSSAKLKATFDDREILTKE
                     AMTHFHLDEEE"
     gene            complement(14520..15176)
                     /locus_tag="EYO68_09745"
     CDS             complement(14520..15176)
                     /locus_tag="EYO68_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08120.1"
                     /translation="MNFKNVQYYSGLFYAGLMILLTSNSQAADPAKLTEPESISTLYE
                     EQSSQTPTIFGGVGRKLPTWQAGLDLLILGTPHYGAFRNQLNLSNRVFWGEWWYNDGW
                     GVKGFLSEQSFRMFGSSGSSAKSSTRHLGVIAKTQHSLSESWKISAGMGLANTEYSLG
                     SQRKQGNSLVSEFRIGMEISDDFWTEAGILTIDSSSGSGSGDQRLGSTGYLIGFSYGF
                     "
     gene            complement(15190..15981)
                     /locus_tag="EYO68_09750"
     CDS             complement(15190..15981)
                     /locus_tag="EYO68_09750"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509827.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIC08121.1"
                     /translation="MDYKTLILEIDERKIAALTLNRPDVHNAMSLEMIRELRKVVGKL
                     NADPEIRAVILSGSGKSFCAGADLRWMQEIAGQKREDRIAEATELSEMLGELDQLNKP
                     LIGRINGLSFGGAVGLIACCDIAVAVEGALFSLTEVLLGLLPATISPFVLRRIGPSNA
                     RRVMLNAHRFSAEEAVNLGLISKAVSADKLDEAIEKEINELLRCAPEAVSATKRLIAE
                     VRGKEPADVRSATIEKLANAWESESIKEGIDAFFSKRKPAWNPEA"
BASE COUNT         4596 a         3585 c         3047 g         4828 t
ORIGIN      
        1 tgactatatt ttaatcatta aaattttctt caaaacatcc agatatactg cactgttatt
       61 tgattagcct tgtaggatcg tgtctcatcg ttggaagcct gcttaatttg cttcaggtct
      121 gcacctgcta gagtcgaact actgaacgaa tatttaaaat ttaaagtcat ctcatctttt
      181 ttgttctgca aaaccagatt tgatgccgga tcagcatttt ggtattgctt attattattt
      241 tgaaaattaa aaccaaaaac ccacggagtc catgaatata tagtacctac attaatcagt
      301 gttgactgat aatccttgct ggcatcttca aattgtgtag actgtgaatc aattcgcgcc
      361 tgaaatctaa agtcttccca tcgcattttt gctcctaaag aagccagtgt caatgacccg
      421 tcttctgaat ttgtttttac cttattctga ctatcaatct gcattttcag agatccggaa
      481 aatttgctcc cccaatgttt tttgctgtct acaaagagaa aaagagactg taaatcttgc
      541 ctgaattcac ctgctttttt aggatccgga ggatttgcat ccttgacgct gattacgctc
      601 tgacccaacc cggttttcaa atcaagaccc agcaggtttt tgtgtctgta ctctgtactg
      661 aactgccgga acaaaacttc ttcagcagcc aggctccggt tttggtgccg gtcaaatttg
      721 agaacagcat ttatggaaac agctgattcc tcattcagtt caaatgcata tgcggccgac
      781 agcaataaat cattttttga tgcacttgtt tttgatttac cttcctgggc actcagaata
      841 ttgtcactgt ttgtttgccc aaacattacg acaccgttca acggaggcgg ttttttcctt
      901 tccttctttt gaggttcata agtgacaata aattcctgtt cactctgtcc tgtttctgtc
      961 tgtacaaaaa tcgtgaaaag attcttcccg gtttttaaat aataaggaac ttcaatctca
     1021 gcccagtctg catcctcagt taccatttgc gcaaatccgt tgaccttgat caccatgatt
     1081 ggactgaagg tactgacctg aaaccggaac cagccctctg caagctcctc ttttcccggt
     1141 gacgtgcgaa aaatcagaat ttcttcagga acctccgaac tttgctcatt ttcattcgtt
     1201 tgcgcgttta tagaattgat ccccaccata ataacaaaaa accagagaaa aacgaaggcc
     1261 cgcaaatgca aatagtacat agctttcctt agcctgcatt aggttcagga aaaaattttc
     1321 tggttcgttc ttttttagtt ggacgagtga aaataagttc tacccgtcgg tttgctttac
     1381 gagctttctg taattctcta ccgctctttc gattgccgtt ttcactgaca taagatgctt
     1441 tgggtcgggt gtccgcatat ccggaaattg caatgcgttc cggattaaat cctacctttt
     1501 ccatcatcag tgtcgcaaca tttgttgccc gaactgtact cagttcccag ttatttttat
     1561 attttccttt tttactaatg ggcacatcgt cagtatgtcc atccaccctg atcgcatctc
     1621 cttccagctc ataacgattt cggatttcat tcagaaaact gctgagctga accacaactt
     1681 ccttcgaaat ggacgcacct ccgctgagaa acaaaaaact ggagggcaga gaaagtatgc
     1741 agcgcgattt actggcattc agctcaaacg aagcaaatgc ctcattttct ctcaggaagc
     1801 gattgattcc actgcaatct ttcatctcct gagcaccgat gattgtattg acttgtttgg
     1861 aaatcttgcc aacatccgca tttaaaacag aattatcttc catctcaatg atcaatgttc
     1921 gggttgcata caaaggcact tcatcctgga tgacctctat ttccacagtc cctttttccg
     1981 tgtagggata gatctcaatc gttgctccat ccatgaagcg gaaatccttc ctctctttgg
     2041 cagttccagc aaacttgagt ggaatatccg ttttcactcc actgataggt gaaatatttg
     2101 ccgttatatt tgaaattcct ttgtaaagat cccgacgcgc aatctcaaag gttactgtag
     2161 ggagcggttc atcatcctgg atgataatgc tcctggttac gaagttcccg cgttccacat
     2221 ctccccggag cttccctatt tgcactaaga tttcttcagg ctcctcatac agttcatcgt
     2281 ttaccaggca cagagggaaa gacgccccaa actctcctcc agggattatg gctaattccg
     2341 cccctaagac gtaatcaaat tcttgaatct ctttcggatt tttcattaat ccttccattg
     2401 tacacagttc cggatcaact gggcccctcc tcgcgtttcc tgcaaaaaat aaaggaatcc
     2461 tcaaatcttc ctctactctt tcattgagca aaatctccac tttcatgaca cccgcagact
     2521 cttcgacctt tcctgtggcc atgctttcaa tcaggatctg ggttttgtac agacccgttt
     2581 gtttttgacc aagaagtatt tccagttttt ccgtaaacat ctctttcgta tcaaaatacg
     2641 tccctttcat tgacttaaaa cctttgacaa tattttccgg caagcgttcc gtcaaattac
     2701 ttattgtctt ttcactgaca ataaaacctg ttttaagatc cacttgcggt ttattaaaca
     2761 gcgacagata tacaaaaaaa caaaacaata gggtaattaa gtcagcgaaa gtcaccatcc
     2821 acttcgcttc tttacttctg gtcttctttt ctccaaacat gttttcttct tatacgattc
     2881 acttttagta cttagagttc ctgccgacgt ccacaagtta aaactaagat cctagaacca
     2941 taacccctca tccgtactga tcatcaaatt cgtcccgttt atgagggaga aaattgtgga
     3001 gttcctgaag aattgtgtat ggcgcttcac ggcgtgctat gcgatttacg ctttcacgaa
     3061 tcatttccag caaaacaaga ttggattctt tcagctgctt acgttttgat gcaataggca
     3121 ataaaaccat gttagagaaa atcacaccgt aaagtgtggt caacatagca acggacatgg
     3181 cctgcataac tgaacgaata cttccggttt ccgctaacat caggaccaat ccgacaattg
     3241 ttccaaccat accgaatgca ggtgcggcgg tacccataaa atcaaatatt tcaatttctt
     3301 catccgcacg ctgttcatac ataagcactt ctgcttttaa tatttgcgcc agagactctt
     3361 cctcaacttg ttcccgaacg atcagtccct ctatccagcg gcgcatgaac ggattttcca
     3421 ggctgctgag atcatcgttg gtaatacgtt tcccgctgta acgtttttct gcaattatct
     3481 tggttttatc ataaatttcc tgagttgcgg attcagcaac atctgctgca aaaacctttg
     3541 gaatgctgcc aaaactccgt aaagcctttt tgtatggaaa tgcaacaaaa gtaaccgaaa
     3601 tagtgccgcc aaagacaatg aaaagcgatt tcaggtcaaa aaaccgtaaa atttgaatca
     3661 cgtatttgtt atacgaatcc gcgtcaccgg tactctccag ccagagagcc aaattagaaa
     3721 attctgaagg ccacagaatt ccggagagga tgaaagtcgt gccgccaata aagccgaaca
     3781 atgtacctaa tttatctgcg ctttgttgtt tcatgaatcc tttttctccc aaatttttcc
     3841 aagttcgata tgcacatggc gcatgaaaac attccgatga agattcctaa gctgttctat
     3901 ccgtaattta gcctcttcat cttcctcacg ctgtttttca tatccctgca gttgttcctg
     3961 ataatatttc cgtgtttctt cagtatattc cttcatgatt ttttcagtta ctgaagaatg
     4021 agaatcaagt agtaagtacg gaacagaaac ctttgctccg gtacttaatc gtgaacgttc
     4081 aaaacgctcc atcactcttc ccacaaaagc atcatctatg atttcggtgc tttctaaatc
     4141 ttcaaaagaa aggctccgct cccgaattag ttccatagct tcgcttgtat ttttagctaa
     4201 ttcaggattc ttctttttaa gttcatcagt actccctaga atatcgttcc attcctctgc
     4261 acttatctga ccggaaagtt ctccaatacg ccctagccca tcaaaaggca tgggaagatt
     4321 tttagcagac tcagtcagtt catctgccaa ttgctgcagt tcactaggag aaacgcttat
     4381 atccttcatg gtcaatacaa tccccaagga atcctctaca ggcaggccct tgagaatatt
     4441 agccatctca ctggggtcca tctgactaaa aacaatagcc tgttgatggg gtttcatttc
     4501 ttgcagtaga tcaacacgaa gttttggtgg catcttcctg atgaacccaa agtaatcaat
     4561 tttttcttca tcatccccag catctgcctc ctcatccgga agatccagtt cctcttcttc
     4621 ggcctcttct tccggtgcca tggcttcaat ggcagtggca actttaccca aggtgccctg
     4681 cagcggatgt tctttttcct ctttctcacg ggtgctgcgg gagacaaaga aaaacaatgc
     4741 cagtgtcaaa agcagcaaaa ctccaagagt tcctataatt gcccattgcg ctggtgtgat
     4801 tgcatcagtg ccttccggtt tatattccgg aatcaactga tcaactccga gcatttcata
     4861 acctgcagga aaagttgcac tgatttctgc cgcctgcttc agcttctctt gtgcatttac
     4921 tggttcgtca ccattcaaat cagcccttgc tccgacaagc aggtttacat attcaaccag
     4981 tttttcagaa aaatcaggac gttctatttg caatttgtcc aaaacgatcg attgttcaaa
     5041 acgatcctgt gagttcagtt cagaaatttc tgaacgaata cgcatttcca cctgattcaa
     5101 atttacagac ttgttaacag ttaaattaat aattcgataa ctaaacagtg cttgtacatc
     5161 attttcttca gtttttattt tgactcctgg tagaagatca ctatcttttt taatgggttc
     5221 attatccagg actgaatgaa gaattacaga aacatcgata tattcttcac gcagagtttt
     5281 acggagaatt ttgtgtatgc gctcccttat ctccggttca gggcggctgc ggctcaatat
     5341 ttctgcatgc agaatgtcga aattaaaaat tgttaagaca acgatacatc ccgcaaaact
     5401 caacaatttt ttgttcaaaa tacgaaaaat tttcatggac aatttaattt tttttacttg
     5461 aaacaaataa tgccaaaaag attacagttt tttttacatt aaattaacac cccaaaataa
     5521 cctattactc ccaaatagtc aagtgtagat acgattgatt ctcatgtatt aaatactgaa
     5581 ttaattcaat aaacggtatt ggaaatgcat ttgattatta tatcagcaga aaataatatg
     5641 catctgtttc aactgcaaaa ttagattcaa aaatataagt caaatattct gtggaaaagg
     5701 tgcccagaaa ctattctctt gctgacactg tttttttaag taaactgatc tattgctcgt
     5761 gagttattaa ttctagtatt atactgatat tattgattta agcttgacat ttcgattgaa
     5821 aggggatatc tgaaaaagtg ctgatatcgt ataagatccc tcacatatgt tcgggatgac
     5881 actataaaac tcgtgaccgt cgagatctat tactgctgca gcacaatgcg ttttaccatt
     5941 aaaacaacac tattaccttt attcattact caaaatagaa tagggcttaa gaaaagatgt
     6001 ttttgtcatc actgacttga tccgggctcc agatcaaaga tctgcttgag tacaagattt
     6061 gaaacatttc ccctggattc cgggtctatg ctgcgtttcg cccggaatga caatggaaag
     6121 ggaatgcggg ctacagtatg ctctcaagct tctaaaacaa tagaaccctg tccaccggtc
     6181 aggctgaaga gaagcttcag gaatgaattc tttgagaatt ttgagacttt ttacgtgacc
     6241 atcaaatata cgttgcaaaa taaatccaat taagttaggc taggtctcaa tttgacttgc
     6301 aaaaacagcc aatctatttt agagatagat taactaaaaa agatttgcct tgaacattat
     6361 taaggggtat aaaatgctga aatatttttc tatcttattt acacttctga cactcgtttt
     6421 agctggttgc actttctctg atcctgatcc tgaagaaatt caagcacaaa tggaccagga
     6481 acatgcccga ataactgaag aacaggaacg tcaacaggag gaagaagcag aaaaactgct
     6541 ggcacaggaa actgctgctg cagaagagac agctgcaaaa gaagctgaag ccgcacgact
     6601 tgctgcggtt gacaaagaga ctgctttgga taaaattgaa aagttactga agccggcgag
     6661 tatagaagtt attcaaccgg aagagccgaa aagtgaacct agtataatta atacaatttc
     6721 ttcggggctg aagaatctcc tgggctcatc cgttaaagaa caacaagaag aaccgaaaaa
     6781 agctgcagac aacgtttcag cttcggaaaa gaagagcaca accaagttta tgccgaaatc
     6841 acctttggcc atcttttccg cacaaaaact ggtttcctgc aaaatagttc ctgaaaagat
     6901 ggaagctatt cataagcgtc tgctgaaaat aaaggatgtg aacattgccg acagtaaact
     6961 acgggacaag acaaatgaca aattaatgcc ggtcattcac tttgacttcg atcaaataaa
     7021 aataaaaccg gcatacagaa aacttctgcg acagcagagt tcgtgtgtca tgaaggccct
     7081 gaaatcccgt aatgacatga taatacaaat tgaaggacat gccgatgaac gtgggagtga
     7141 tgagtataac atggctttgg gacatcgcag ggccaatgta gtagctaacg caatcaaggc
     7201 ctatcttcca gattcatcac tagcgagaat cttcagtttt ggtgaagaat ttccgctggt
     7261 ccgtctctcc aacaaaaacg cctgggccaa aaacagaaga gttgagttca ctttattgct
     7321 aaaaccataa atacgtgaat ggattttttg accctcccat ttactacaga ttttagtagt
     7381 attgccttta gcgttcttgc gatacttttg gggtgttcgg tgattgtctg gatcacgctg
     7441 ttcatgaagt tcttcagcct cattcgggta aaacatctcc aggcacgcgc actaaaactg
     7501 ctggaaaaca cccagcgtgt gagtgaactc cgcctgcgtt tcagcaatat gcctaaaaat
     7561 ccacttcagg aaatgttcgc acttggcgac cgtgaactga aaatattcct gaaaacaagt
     7621 cctgaatcag actacggcca tcgtagagag ttgatggaca ggcttgaacg aattcttgaa
     7681 gccaggattt tgctggcaga aaaagattta aatgcaggac aagccctgct ggctacaatc
     7741 gctgcaactt ccccgtttat aggactattt ggaacagtaa ttggtgtcat caacactttc
     7801 atgagcatcg cggaattcaa ttcagttgaa ctgtcagtta tcagtcccgg tatagcagag
     7861 gcacttgttg cgactgcagc cggtctgttt gctgcaattc ctgcaacttt aggttacaat
     7921 ttgtttcgtg caaccatccg tggaatgact gagacaatgg attattttgc tcttcaacta
     7981 ctgcaccacc tccaaaaacg tttgttacca aaaaaatgag aaaatttaga catgctagaa
     8041 gactccgcgg agaacttatg tctgaaatca acattacccc gtttgttgat gttttgctgg
     8101 ttctcctgat ttcctttctc atttctgcac cgctactgac gcaggccatt caaattcagc
     8161 ttcctaaagg taaattaaaa gaacgggtac ctgtttacaa agaatcaatt cttgctgttg
     8221 tgaataaaaa aaagcaaata gtaatcagtg atcaaatcta ctcactcaaa agtctggcta
     8281 aatctctaac aaaaaatgcc aacgaatgga aaaggaacca gcctgtatat gttcaaatgg
     8341 atgaacaggt gccttatgga attttaatgg atttgatgct tttgtttaaa aatgcaggct
     8401 tcccccaagt tggacttgtt tttaaaaaag aatcctaggt caaagttttc cggcaggctg
     8461 aaattataaa agtctccaac taaaagctaa atgaaattaa tgatggttgc cggagaagtc
     8521 tccggagatg cccatggtgc aggtttgatt cgcagtttgc ggaaaaacca tgacagtgct
     8581 gatattttcg gtattggcgg taagcagatg ttaagagagg ggttccgtcc atacttcatg
     8641 ctggactccc tgcaaacaca cggtttagta gaggtggttc agcatcttcc aaggctttat
     8701 caaaccctct ggagaatgcg tcaggctttg acggaagaaa aaccggatgc attgatttta
     8761 atcgattatc cgggattcaa tctaaaactt gcagcttatg ccaaaaaaac aggtgttccg
     8821 gtaatctttt tcaacagtcc ccaaatttgg gcctggcgaa aaggcagatt gaataccatc
     8881 aaacgtgtag tggacaaaat gattgtcctc tttccttttg aagaaaagat ttatcagcaa
     8941 gccggcgtcg atgtaaaatg ggttggacat cctctgctgg atgaaatgca gagcgaaatc
     9001 gattccggag caatccgcaa acagttcgga ctgcagaaca acatcctgct tatagcaatt
     9061 gcgcccggaa gccggccaag tgaaatgagg agacaccttc ctaccctgct tgaagcactt
     9121 ccgcaaattc aacaaaaaac cggtgagatt cagtgcatac tgcctgttgc agaatcacta
     9181 gacttaaaag aaatcaggaa actgacagac acatcatccg ttccagtcac cctggttccg
     9241 gaacagttta ttgaaactat tcaggcttgt gatgctgcaa tagtcgcatc cggaactgct
     9301 tccctgcaaa ctgcacttgc acttaaacca ttcgtgattg tttatcaagt ttctccgcta
     9361 acttaccgga ttgcacgtta tctgtcggaa actgaatatg ttggcctggt taatgttttg
     9421 gctgaaaaag aaattgtacc tgaattactg caaaacaatt tcacagtcag taaagttact
     9481 gagattgcag ttcgtctctt gcaggatgag gactatcgtg ccacaatgat cagccaactg
     9541 aaatcaacgc gcacaaaatt gggtgaagct ggagcatatg acaaagctgc acggtgcatt
     9601 gaaaatgttc tgagtagaga accagccatt taaaaactgc aaatcaagaa tcactgaatt
     9661 ctatgaaaac tgctgaatta tcactcgata cccacaatat tgcccaccac ctgacacaaa
     9721 ctttgactgg agttgaagca gtgaaacaac tgtttgaggg acagatgggc attgataaga
     9781 cagtagtacg tcagttattg agcgcagcgc tgcaccgagg cggcgacttt gctgatattc
     9841 actttgaata cagtacgcga aactctgtgg tcatggagga tggcatcatc aaaaactctg
     9901 cagttgcagt ggtgtccgga gttggaattc gagtggttca gggtgatcaa accggctacg
     9961 catacagtga agattttgac ctgaaaccaa tgctgcatgc cgcacgaact gccgcagcca
    10021 ttgcttccag cggtgaggtt tctctgagtg aaagttttcg cttcaatgaa ctggttccac
    10081 gtgactatta tccggtactg gaaactgtga cagatttaga actggtgcag aaaatagaca
    10141 tggtaaaacg cactgaagct gtctccagag cctatgatga gagaataatt cgagtgactg
    10201 tagccatgat ggatgcactc aacctgacac aggtcgtaac gtctgagggt tcaattttac
    10261 gcgatacacg accgatgttt cgaatgaatg ttcattgcgt ttcacaggaa gctgacaata
    10321 tccaaaacgg ttcttctgga atcggaggac gagttggctt ggattttttg aagcagaaag
    10381 accatgcagt gaatttagga aaacaggctg cttcagaagc aattttgctt ctgggcgcaa
    10441 aacaggcacc ttccggatta atgcctgtta ttttaggacc ggcacaatcc ggaatactgc
    10501 tgcatgaagc agttgggcat ccattggagg ctgatttcaa tcgcaaaggg acctctgctt
    10561 acagcggacg tattggtgaa aaagtagctt cagacttgtg ttcgatctat gactccggga
    10621 ctattccgca cgaacgaggt gcaataaact ttgacgacga aggagttcct tctgcagaaa
    10681 atatattgat cgaaaatgga gttttgcgaa actacatgca tgacaggatc agcgccagac
    10741 acttccgcac caatcctacc ggaaacggac ggagggaatc atatgcgcat tatccaatac
    10801 cacgaatgac atcaacttac ttggcgaatg gggaatctga tccggaggaa ataatcagtt
    10861 ctgtcaaaaa aggggtttat tgtcaggaat ttagcggtgg acaagtggat atttccaatg
    10921 gtgattttgt attcgtacca acggtagcct ggttggtgga agacggtaaa aaaaccgtgc
    10981 cgataaaaaa tttcacctta attggaaatg ggccggatgc aatgtcaaaa gtaagtatgg
    11041 tcggtaacga ttttgccatg agtgagggaa tctggacctg tggcaaggaa ggacagagtg
    11101 ttccagtggg agttggactt ccaacagtcc tgatttcgga aatgactgtc ggtggaatgt
    11161 agagaacaag aatctaatat acgtccggct caaatacaaa atacgttggg tttaacgtga
    11221 tagcattcct tttgttatcc ccgacttgat ccggactcca tgaacctgac tttgtcgata
    11281 acgagctttg aaaggccccc ctggattcct ggtctgcgct tcgccagttc aactttttat
    11341 ttccaagcca aattggttct tattctttct tgagtttatc tgcagtcagg tttgaaattt
    11401 cacaactaag tactgcaagc tcaagacgat ctaaaatatg tttatcaagg agtggctgat
    11461 aatttccttc agtaccagat ccggaacttg aaactgaatt agtcagctcg tttttttcga
    11521 caaacaattt tagttgtttc gctaaatgag tggtttgtga tttgattttc tttagttgag
    11581 cagatgcttt tatccaatct ttagaaaaac gtgccatttc agcaagttta ttaaaactaa
    11641 agctatcgtc agttttaacg tgcttgcccc ataattctcc taaacccaga gagccagcct
    11701 tatcactcat cctgtccatg aaggactgat tgtttttaaa aatatcaaac agatcaatag
    11761 tgatgatctt ttcctctcca gctcccttac ttattgggcg tttcaattct ttaagtctca
    11821 actcacgaat cttaatatcc ttgagaaatc ttttacgggc actagtctgt aactgatatt
    11881 catcttcatc atcaaaatgt gatttttcca ttttgttaat gacacgcatt acctcctcca
    11941 atttttcttt gactaccact aaagctttct cttcagctgc aatttttcct tctgcctggt
    12001 ctttctgctg aactttcctc tcttctttct tactgataat tgcttttttg cgtaatgcaa
    12061 ctctgacagg aaaagagtcc tcggtaaatt tccatgaatt aaaaaataca atttcaatat
    12121 gttcaaagtc cggcgaatta aaaatgaatt cttcaatatt ggctttggca aactcgtcct
    12181 cagcatgtgc ttttctaaac tcctgaatct tttttgaaag ttcacttgta gacataaatg
    12241 aaaggaattt ccgtttaaaa tcaagggcaa caagaccatc aattgtacgc ttttccatat
    12301 catcgatgat catcacgtga accaggccct tcatcagacc gtctaaaact gcaattccag
    12361 tgattttttc aatagatgta ctgagaatat agccaattcc tttgtagtaa ggtttttcaa
    12421 cctgagaaat tttctgctca agacggtttt ctaaatcctc aaacttttgt atcaattgct
    12481 gtttgccgcg caaaacagca tcaatcaaag ccaatttgac aaaagccggg tgtttctctc
    12541 gatctataca agtttgctgt acttccttgt agcgcccaat taattttgtc agcttcactg
    12601 aatcacttga aggcaatttt tccatctgca attttggcat gcccggaaca cggaaaatca
    12661 aaccgagact gctcgcatca tgagctttcc cttttggaat atctcctgga aagcttaaaa
    12721 tccagtgaaa ttttttctgc ttctcttctg gataattcgt ttcatcaact gcaactgtta
    12781 cacctgaatc atgtaagcga tccaccaaag tcttcatgta aattttgcgc agatcttttc
    12841 tgagaagcag caggattagt ttctctttat tcccgttgcc ttcctgatcc cgtttgtctt
    12901 gtgcttggtc agccatattt gcattactct tcttcgtcca gatgaaaatg agtcattgcc
    12961 tccttggtaa gaatttcacg atcatcaaaa gtggctttga gtttagctga acttactttc
    13021 agttccttaa actcttcagt accttcttta agttttccat cagtgtcata gttgtccaaa
    13081 aatttcatat actgtgcagc ggactgtaat tcatttaggt agattttaat ctgaaataat
    13141 aatacacatg tatgtagaag atggaggcct tccagacttt caaaaatggg ttccaatgta
    13201 tcaacagttt ttttcaagtt tgcttcagca gcatccctgt tcttggattc catcaacatt
    13261 tctgcttgaa tcatcagcat tgaagctttt ttacggagag ctgcaccttt cacgttgtca
    13321 tttgaagcaa tgctggccat ttgttcaaag gttgcaattg ccaaatcata ggaaagaatc
    13381 ccctgctgta tgtctcgttc ttccccgtaa aacgtttctc ctatttcctg tgcttttttc
    13441 agcaatggtt caattttttc ttcccatcct ttgctaaact cctctactga aaatgttggc
    13501 tcagatgtct tcagacgttt aaaatgttct ttgtcgttac tggagaaacc gcatttagag
    13561 caggaagcaa cttgaatgag attaaaatca caaaatttca actcacctgc agacctgaca
    13621 aaagaagata caccaaaaat atttggatca gtaatcatac ttttggagtg caggacatag
    13681 aatttgatct ttggattatc gcaaacataa caatccaaaa aactacccaa tagtgaatct
    13741 ttctctgtat caaaaaatcc gccgtctgaa actacagcat aatcttcagg atgcataatc
    13801 tgcataattc catgatcttc aaataatctt tgctcaatat gtgctcgtac cacataactg
    13861 ccttcatcaa atttaacctg atgttgtttt tccattttgc agtcaagagg atcccagtgt
    13921 tcttcatttc cttttaatgg aagttcagaa tggacactct taagaacata aagcattatt
    13981 tttacttttg gcatcagggc ttttcccaac cgaaatgtga ctccagtttc atcaactttt
    14041 gtcaaactca ctttagtctt taaactccct gtttctacaa ttgcttgggg caagtccctc
    14101 tccagaagtg cacgggcgct tttccatagt ttagtcaaaa tttcattatt aaaactcaat
    14161 gatcttcccg ctaagaggaa ataactaatt tcttttggag ataaatcagc atctgcgacc
    14221 atcaactgtg caagatattt aagcatcagg aaagcttgct ttgggtctat atcgaggggg
    14281 cccaactctg gaggattgcc atttttaatg ataaccatca atttatcaat ttcatctttg
    14341 tcatccataa aattgatggc ctcccggaga aaactcattt cagactgatc tgcatgacca
    14401 tcagcaacaa ccattcctgc aatagagtta gcaaaccaca ttttttcttc cgctgacatt
    14461 tcatctaaat ttggtaactg catgcttttt ttctataaaa ttaatttact gacttccatt
    14521 taaaacccgt agctaaagcc tatcaaataa ccagttgaac caaggcgttg atcaccggat
    14581 ccgctgccgc tggaactgtc aatggtcaat attcctgcct cggtccaaaa gtcatctgat
    14641 atttccattc cgattctgaa ttcagagact aaagaattcc cctgtttacg ctgacttcca
    14701 agactatact ctgtatttgc cagccccatg ccggcactta ttttccatga ttcagacagc
    14761 gaatgctgag tttttgcaat gacacccaga tgcctggtgc tggattttgc actgcttccg
    14821 cttgaaccaa acatccgaaa ggattgttct gaaaggaaac cttttactcc ccaaccgtca
    14881 ttgtaccacc actcgcccca aaaaacacga ttgctcaagt tcagctgatt gcggaacgcg
    14941 ccgtaatgcg gtgtgccgag tatcagcaaa tccaggcctg cctgccaggt cggtaatttt
    15001 ctgcccacac caccaaaaat tgtgggagtt tgagaagatt gttcttcata aagagtagag
    15061 attgattcag gttcagttaa tttagcggga tctgcagcct gactattgct ggtcaaaaga
    15121 atcatcaaac ccgcataaaa caatcctgag taatattgaa catttttaaa attcattttt
    15181 tctttacatc taagcctcag gattccaggc cggtttgcgt ttgctgaaaa aagcatcgat
    15241 cccctctttg attgactcag attcccaggc atttgccagc ttttcaattg tagcactacg
    15301 cacatctgcc ggctcttttc cccggacttc tgcgatgagt cgctttgtag ccgaaactgc
    15361 ttccggagca cagcgcagca gttcatttat ttctttctca atggcctcat ccagtttatc
    15421 agcagaaaca gctttggaga tcaaacccaa attaacagct tcttctgcgg aaaaacgatg
    15481 tgcgtttaac ataacacgcc ttgcatttga cgggcctata cgccgcagga caaatggtga
    15541 gatcgtggcc ggaagcagac ccagcaaaac ttcggtcaat gaaaacaagg ctccttctac
    15601 tgcaactgca atgtcacaac aggcaatcaa gcccacggca cctccaaaag ataaaccatt
    15661 gatccgtcca attagtggct tgttgagttg gtccagttct ccaagcattt ctgagagttc
    15721 cgttgcctct gctatcctgt cctctctttt ttgtcctgcg atttcctgca tccagcgcag
    15781 gtccgctccg gcgcaaaaag actttccgga accgctcaga ataacagccc gtatctccgg
    15841 atctgcattt aattttccaa caactttccg cagttcacgt atcatctcca gactcattgc
    15901 attgtgaaca tccggacgat tcaaggtcaa agcggcaatc ttgcgttcgt ctatctctaa
    15961 aattaaggtt ttataatcca ttttattttt gctgaaatta cttgaaactg aaaagtggta
    16021 ttatccactt ttcagcaata acggcaagtt tttgct
//
LOCUS       DTVD01000260            4402 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7078_length_4402_cov_237.885668, whole genome
            shotgun sequence.
ACCESSION   DTVD01000260 DTVD01000000
VERSION     DTVD01000260.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7078_length_4402_cov_237.885668"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="EYO68_09755"
     CDS             complement(<1..1034)
                     /locus_tag="EYO68_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIC08122.1"
                     /translation="MKAAVCHEFGKPLVIEEVELLPPKTGEVQVKLAACAICHSDILY
                     AEGAWGGDLPAVYGHEAAGIVESVGHGVKSFQPGDHVLVTLIRSCGTCYYCTQGNRVR
                     CETVFPLDEQSPLRTADGKPIAQGLRTGAFAESVVVDVSQLAPIPEDVPLDSASVLSC
                     GVITGFGAVVNTAQIPAGCSVVILGTGGVGLNSVQGAAVAGAEKIIALDLSDAKLEAA
                     RNFGATHTVNVSQQKATKAVYGLTGGRGADYVFVTVGAKSAIEQGFKLLGRGGTLVVV
                     GMTANGVKIEFDSCKFASIEQRLLGSKMGSTRLATDIPWYISLYQQGRLKLDELISGR
                     YPLEQINEAIS"
     gene            complement(1039..1821)
                     /locus_tag="EYO68_09760"
     CDS             complement(1039..1821)
                     /locus_tag="EYO68_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512008.1"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HIC08123.1"
                     /translation="MAEAPFTLNHRGPILEITLSKGKANVISAADSRELNGMWEAFRD
                     NPEQRFAILTGKGEKFFCAGWDLKNVAESGEASDSDYGSGGFGGLNYPRNFNKPVICA
                     VNGIACGGGFEVMLGCDIIVAEEHATFALPEIKVGVLPDCGVVKLRRRIPYHVAVELM
                     MTGRWMDAEEAKHWGLVNHIVPKGQGLDKAREIAEALADGPPLLYPAIKQVLRMTEMV
                     PENEAFTVHDACSAVEAVNRSDDLKEGAKAFAEKRNPVWKGK"
     gene            complement(1866..2816)
                     /locus_tag="EYO68_09765"
     CDS             complement(1866..2816)
                     /locus_tag="EYO68_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIC08124.1"
                     /translation="MKIGFIGLGNVGGKLAGSLLRNGYELTVRDLNREIVSPFLEGGA
                     NWGESPKQMAEATDLLITCLPNPTASAVVMEAEDGILAGLSSGKIWAEMSTTDEVEVK
                     RLGKRVQELGGEALDCPVSGGCHRAETGNISIFAGGERSSFEKILPVLKVLGRNILHT
                     GALGSASVLKVLTNYLATANLVTLCEALTTAKMAGMDLSTTYEAIRISSGNSFVHETE
                     SQVILNGSRDINFTMDLVLKDISLFQSIAERAGVPLEVSPLLIDIFKDGEQRYGSREW
                     SSNIIRRLEDACETQILASGFPAQMTDDEPEVSGEEVVVQ"
     gene            complement(2870..3733)
                     /locus_tag="EYO68_09770"
     CDS             complement(2870..3733)
                     /locus_tag="EYO68_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08125.1"
                     /translation="MSKQISCLEMINRIEYPIDELEHHKRQQAIDLVRKELSTDGCAV
                     IRKFFSKGGLNALLSEAHELSEQAYYSPKKQCNVYLGDGNPAMTEDNPQNIFLERSNG
                     FITADLFGEESASRRLYYWEPLKRFLADCLEKEELFIYEDPVSNMIVNVAKPGQRFNW
                     HFDTNEFTITILLQAAESGGIFEYVPNLRSRDNECIEEVKKVLSGDHNRVKQLELKAG
                     DLQFFLGRFSLHQVSENTGNTDRLLLIQSFAEKPGMIGSMYRVQDLYGKTAEIHIEHE
                     QNKNRADNLLD"
     gene            complement(3800..>4402)
                     /locus_tag="EYO68_09775"
     CDS             complement(3800..>4402)
                     /locus_tag="EYO68_09775"
                     /inference="COORDINATES: protein motif:HMM:PF13640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIC08126.1"
                     /translation="YFCHQNHNAYLLDPDPDFPAEHIRNLEQVSDKGCITHDQVPENS
                     LLRTLYEWPDFRKFLKQVLGEPVFPYADTLSSININYYEQGQQLGWHFDNASFAVTLM
                     LQSPGQGGEFEYREKVRNSEKDDSAFADTEAVIKGKLQPKTLAMGDGALVLFRGRNSL
                     HRVAPVVSEHARILVTLNFNTEPGIMLSELARMAFFGRLA"
BASE COUNT         1105 a         1128 c          926 g         1243 t
ORIGIN      
        1 gaaatggctt cattgatttg ttccaagggg tagcgtccgg aaatgagttc gtcgagtttg
       61 agccgtccct gctgatagag tgaaatgtac cagggaatat cggttgccag ccgagttgac
      121 cccattttgc tgccaagaag acgttgctcg attgaagcaa atttacagga atcgaattca
      181 atctttactc catttgccgt cattcccact acaaccagag ttcctcccct gccgagcaat
      241 ttaaaaccct gttcaatcgc actcttggca ccaactgtaa caaacacata atcggctccg
      301 cgtccacccg tcaaaccata aacagctttt gtagcctttt gctgacttac atttactgtg
      361 tgtgttgctc caaaattccg ggccgcttcc agctttgcat cagatagatc cagagcgatg
      421 attttttcag caccggctac ggcagcacct tgaacactgt tgagtccgac cccaccggtt
      481 cctaagataa ccacgctgca tccggcggga atttgtgcag tatttacaac tgcgccgaat
      541 ccggtgatga cgccacaact caaaacggat gcgctgtcca gtggaacatc ctcgggaatc
      601 ggagctaatt gtgagacatc aaccaccact gattcggcaa aagcaccggt tcgaagtccc
      661 tgcgcaatcg gctttccgtc agccgtccgc agcggagatt gctcatccag cggaaaaacg
      721 gtttcgcatc gaaccctgtt gccctgagtg caatagtagc aggtgccgca cgagcgaatc
      781 aatgtcacca aaacatgatc gcctggctga aagcttttta ctccatgacc cacactttcc
      841 acaattccgg cggcttcgtg tccataaaca gcagggagat ctccgcccca tgctccttcg
      901 gcgtacagaa tatcgctgtg gcaaatggca caggcagcga gttttacctg tacttctccg
      961 gttttcggcg gcaggagctc aacttcctca attaccagtg gtttaccaaa ttcatggcat
     1021 actgctgctt tcatgtgctt attttccttt ccatacaggg tttcgttttt ctgcaaaagc
     1081 ttttgcacct tcctttaagt catcggaacg gttgaccgct tcaaccgcag aacaagcatc
     1141 gtggactgta aaggcttcat tttccggaac catttctgtc attcgtaata cttgtttaat
     1201 cgccggataa agcaacggcg gcccgtctgc aagggcttct gcgatttcac gggctttgtc
     1261 cagtccctga cctttgggaa cgatgtgatt gaccagtccc cagtgtttag cttcttcggc
     1321 atccatccag cgtccggtca tcatcagttc gacggcgaca tgatacggaa tacgccgtcg
     1381 taattttacc actccgcaat cgggaagcac cccgaccttg atttccggca gggcgaaagt
     1441 tgcgtgttct tcagcaacga taatatcaca acccagcatg acttcaaaac cgcctccgca
     1501 ggcaatgccg ttgaccgcgc aaattacagg tttgttgaaa ttacgcggat aattcaagcc
     1561 gccaaaacct ccggaaccat aatcagaatc tgaagcttca ccggattctg ctacattctt
     1621 caagtcccaa ccggcacaaa aaaacttttc tcctttacct gtgagaatgg cgaaacgctg
     1681 ttccggattg tcccggaagg cttcccacat cccgttgagt tcacgactgt ctgcagcgct
     1741 gattacattg gctttgcctt tggaaagtgt tatttccaaa atcggtccgc gatgatttag
     1801 agtaaacggt gcttcggcca taaggtctcc ttcattaaaa tgagtttctt gtttcggcag
     1861 gttttttact gcaccaccac ttcttctccg gaaacttccg gttcatcatc tgtcatttgt
     1921 gccggaaatc cggaagccag gatttgtgtt tcacaagcat cttctaagcg gcggataatg
     1981 tttgacgacc attcacggga tccgtaacgc tgttctccat ccttgaaaat gtcaatcagg
     2041 agcggggata cttccaatgg aactccggcg cgctctgcaa tggattgaaa gaggctgatg
     2101 tctttgagaa ctaaatccat tgtgaagttg atgtcccggc tgccgttgag aatcacctga
     2161 ctttcggtct catggacaaa agaatttccg gaggaaattc ggattgcttc atatgttgtg
     2221 ctgagatcca ttcctgccat ttttgctgtt gtcagtgctt cgcaaagtgt gacgaggttg
     2281 gccgttgcca gataatttgt cagtaccttc agtacagaag cagaaccgag tgctccggtg
     2341 tgcagaatat tccgtcccaa taccttcagc acaggcagga ttttttcaaa actggaacgt
     2401 tcgccgccag caaaaattga gatattgcct gtctctgcac gatgacatcc tccggaaacc
     2461 ggacagtcca acgcttcgcc tcctaattcc tgaacccttt ttcccagccg tttgacttct
     2521 acctcgtctg ttgtactcat ttccgcccaa atctttccgg aagaaagtcc ggcaagaatc
     2581 ccgtcctctg cttccataac gactgcactt gccgtcggat taggtaaaca ggtgattagt
     2641 aaatctgtag cttcggccat ctgttttggt gattcacccc aatttgctcc tccctccaaa
     2701 aaggggctga caatttctct gttaaggtct cgcaccgtca attcatagcc gttgcgtaaa
     2761 agacttcccg ccagctttcc tccgacgttg ccaagtccaa taaatccaat tttcataatt
     2821 ttccttcaaa agaggagttg tagttgtttc ttgtttttta taagaggcct caatccaaca
     2881 gattatctgc ccggttttta ttctgttcgt gttctatgtg tatctcagcg gtttttccat
     2941 aaagatcctg gacacgatac atgctgccaa tcatccccgg cttttccgca aaggactgaa
     3001 ttaggagcaa acgatctgta tttccagtgt tttcactgac ttgatgcaga gaaaaacgac
     3061 ccaaaaaaaa ctgtaagtca cctgctttca actctaactg tttcaccctg ttatggtcac
     3121 cggaaagaac ttttttaact tcttcaatgc actcattatc ccgagatctt aagttgggta
     3181 catactcaaa tattcctcct gattcagcgg cttgcaaaag aatagtaatc gtaaattcgt
     3241 tggtatcaaa atgccagttg aatctttgtc ctggcttggc gacattcaca atcatattgg
     3301 atacaggatc ttcataaatg aacaattctt ctttttccag gcaatccgcc aaaaaccgct
     3361 tgagaggttc ccagtaataa agtctccttg aggcactttc ctcaccaaaa aggtctgcag
     3421 tgatgaaacc gttgctacgt tcaagaaaga tattttgggg attgtcttcc gtcattgcag
     3481 gatttccgtc acccaaataa acattgcatt gtttttttgg agaataataa gcttgttcac
     3541 tgagttcatg tgcttcactc aacagggcgt tcaaacctcc tttgctaaaa aattttcgta
     3601 tcacagcaca tccgtctgtc gacaattctt tccggacaag atcaattgcc tgttggcgtt
     3661 tgtggtgttc caactcatca attggatatt cgatacggtt gatcatttcc agacaactga
     3721 tttgtttaga catggaattc actgtttcta atggatgtta aaaccaatct tttttgtcaa
     3781 caatagtatt tattcccatt cacgccagcc gtccaaagaa ggccattcgt gccagttctg
     3841 aaagcattat tccaggttcg gtattgaaat tcagcgttac aagaattcgg gcgtgttcgc
     3901 tgacaactgg agccactcga tgcagagagt ttctgccacg aaagaggaca agcgcgccat
     3961 cacccattgc cagagtttta ggctgaagtt tacccttgat cactgcttct gtatcagcga
     4021 acgccgaatc gtctttttcg ctgttacgca ccttttcccg atactcaaac tctccacctt
     4081 gcccaggaga ctgaagcatc aaagttactg caaaggatgc gttgtcgaag tgccatccca
     4141 gttgctgacc ttgctcataa taattaatat ttatggaaga aagtgtatct gcataaggaa
     4201 agaccggctc ccccaaaacc tgtttcaaaa actttctgaa atcaggccat tcataaagcg
     4261 tccttaacag agagttctcc ggaacctgat catgcgtgat acaacccttg tcactgacct
     4321 gttctaaatt acgtatgtgt tcagcgggaa agtcagggtc cggatccagc aggtaggcgt
     4381 tgtgattctg gtgacaaaaa ta
//
LOCUS       DTVD01000261            4401 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7083_length_4401_cov_374.183387, whole genome
            shotgun sequence.
ACCESSION   DTVD01000261 DTVD01000000
VERSION     DTVD01000261.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7083_length_4401_cov_374.183387"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(29..1912)
                     /locus_tag="EYO68_09780"
     CDS             complement(29..1912)
                     /locus_tag="EYO68_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIC08127.1"
                     /translation="MNQIKKIKFVFQEVLRLFLIFVLVFQIFRIAIYYSYRDLFNNLD
                     FLKLTESLFLGLRFDLSSTSILLFIPIVLLIFPLRITGHLFFRRFVASVIYLELVAMI
                     IFLTSDYMYFSFVKRHITNELLFLLNDSEYLMTEVSVKLLPIIFLIVLTILFYPLFLK
                     VTCPKKPEVQRSILSFVLILLVLIVVGRGGFQRKPIAVIDAYQYGSASQGHLILNGIF
                     TASHFSISSKFIERTAGEEKLYLDTLDLPVSTTPDYPLERTNVQSGMSPKKNVVMIMI
                     ESLSFKYIDYLSGQNYGVTPNIDRFARNGLVFENFFANGQRSVDGAQSILTGIPPLPG
                     MPDITALSVNYSSLGQLASDNHYRTIFLTTTLRESFSMDLIAGAAGFDDYHGLEDYPL
                     LLNYIDAADRPLGWDYEAFMYLLKQLEGEKSNYVAFVNASSDHTPFARMQEPFNQYEH
                     GTDTEGGYLNMLHYTDWALGEFINEYKKRKDFEETVFIITADHALPHFQGAEPYGKFR
                     IPLIIYSPKNISPGRSQMFASQIDLFPTIIKLLDLEGKYSAIGKNILDTEKSFAVVKD
                     GALLEIFSKEGFLVHNLKSVLEFQPVDALSDEFTKTKLASKAVAFDHLTYLLLTQNRW
                     LSP"
     gene            1929..2921
                     /locus_tag="EYO68_09785"
     CDS             1929..2921
                     /locus_tag="EYO68_09785"
                     /inference="COORDINATES: protein motif:HMM:PF01066.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08128.1"
                     /translation="MSSYQYRCSDESILLPFLKKNVFSVLHRYIPYGVPANYLTLVSI
                     FVIWSCFLYFIGIDTADEGDIIIAFFAIIIYVIFDHFDGLQAKITATGSPLGEILDHY
                     SDVFNGSIILYLFFRILQIELDWIFYLAIWLNLVAFTLTYLEQSIQKELHFGKIGSLE
                     GVVLILLIICSFMTSQGKTFWLEYTMLDMPVYVFLVLGLIMGVIYTVVGSLRRLQHIP
                     KSFIHYLLTGSILYFLCIYYQINWYIAFLVINVYSADLILKSMKSHLLDAESPKPDFI
                     VYILFLFLIYLSFFEYEQQIGFLVYGLVVTAKIIWNMVQIFSKFRNYWVWWNLH"
     gene            complement(3437..>4401)
                     /locus_tag="EYO68_09790"
     CDS             complement(3437..>4401)
                     /locus_tag="EYO68_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIC08129.1"
                     /translation="VTTELATYAPGKTKNPHDSNRTPGGSSSGSAAAVASYMVPIALG
                     SQTNGSIIRPAAFCGVFGFKPSFGMIPRSGVLQQSLPLDQLGVFARTTDDVALLTQVL
                     AGYDETDSATTFLRATPSLISVAAEEPPLPPKIGFVKTPVWDRADEMCKGAHEELCEM
                     LSENVEEIELSPSFGHVLDHHKIIMEADLAYSFAHDYETAKDKLSDSLRLQIERGMSY
                     SAVAYHQAMSQQAVLDDILGGLFQEYDAILTPSVSGEAPIGLDNTGDPSFCTIWTFCG
                     VPALNIPVFQGENGMPIGTQLVGAKNDDARLLRTANWLLSKLND"
BASE COUNT         1270 a          910 c          916 g         1305 t
ORIGIN      
        1 tgcagacact tttaatttag cgacacgttt aaggactcaa ccatcggttt tgagtcagta
       61 gcaggtaagt cagatgatca aaagctaccg cctttgatgc caacttagtt ttagtaaatt
      121 catctgacag ggcatccaca ggttggaatt caagaacact tttaagattg tgcaccaaaa
      181 acccctcctt ggaaaaaatt tctagaagtg ccccatcctt gacaactgca aaactttttt
      241 cagtatctaa aatatttttc ccgatggcac tgtactttcc ctccaaatca agcagtttta
      301 ttatagtagg gaacaaatca atctgtgagg caaacatttg ggatctacca gggctaatgt
      361 tttttggact gtagataatc aaggggatcc ggaacttacc ataaggctct gccccctgaa
      421 aatgtggaag agcatggtct gctgtgatga tgaataccgt ttcctcaaaa tctttacgtt
      481 ttttgtattc atttatgaac tctcccagag cccagtccgt gtagtgcagc atgttgaggt
      541 aacctccttc agtatctgtt ccatgctcgt attgattgaa aggttcttgc attcttgcaa
      601 atggagtatg gtctgaactg gcattgacga aagcaacata gtttgacttt tccccttcaa
      661 gctgttttag caaatacatg aaggcttcat aatcccaacc caaaggacga tctgcggcat
      721 caatgtaatt cagtaataac ggataatctt ccagcccatg gtaatcatca aatccagcag
      781 ctcctgcaat aagatccatt gagaacgact ccctcagggt ggtggtcaag aatattgtac
      841 ggtaatggtt atcactagcc aattggccca gactagaata attcacagat aaagcagtga
      901 tgtcaggcat gccgggaaga ggtggaattc cagtgagaat cgattgggcc ccgtccacac
      961 ttctctgtcc atttgcaaaa aaattttcaa acaccaatcc attgcgggca aatctgtcaa
     1021 tgtttggagt caccccatag ttctgtcctg aaagataatc aatatattta aaagacagac
     1081 tttcaatcat gatcatgact acgttttttt ttggactcat gccggactga acattggtcc
     1141 tttcaagagg gtaatcaggg gttgttgata ccggtaagtc cagagtatca agatataatt
     1201 tttcttctcc tgctgtccgt tcaatgaact tactggagat gctgaagtgg gatgctgtga
     1261 aaatgccgtt tagaatcaga tgaccctggc tggcagagcc atactggtat gcgtctataa
     1321 cagcaattgg ttttctctgg aatcccccgc ggccaacaac aatgagtacc agtaaaatca
     1381 gaacgaaact caaaattgag cgttgaactt ccggtttttt tggacatgtc actttgagaa
     1441 acagtggata aaataaaata gttaaaacta ttaggaaaat gataggcaaa agcttgacag
     1501 acacttcggt catcaaatac tctgaatcat tgagcaaaaa tagtagttca ttcgtgatat
     1561 gccttttgac aaagctgaaa tacatgtagt cagaagtcag gaagatgatc attgcgacca
     1621 actccaagta aatcacgctt gccacaaacc tgcggaaaaa cagatgtccc gttatccgga
     1681 gtggaaaaat cagaagaaca atgggtatga aaagcaggat agaagtggag gacagatcaa
     1741 aacgtaatcc aagaaaaagg ctctcggtta atttcaaaaa atccagatta ttaaataaat
     1801 cacgatatga ataatatatg gcgatcctga atatttgaaa aactaagaca aagatcagga
     1861 ataatcttag gacttcctga aaaacaaatt taattttttt tatttgattc aatttaattg
     1921 cattgttaat gagttcttac caatacaggt gttcggatga gtctatcctg ctcccattct
     1981 taaagaaaaa tgtcttttcg gttcttcatc gctacatacc ttatggtgtt cctgccaact
     2041 acttaacttt agtctcaatc tttgtaattt ggtcttgttt cttatatttc ataggcatcg
     2101 acactgcgga tgaaggggat atcattatcg ctttttttgc aataatcatc tatgttattt
     2161 ttgatcattt tgatggacta caggcaaaaa taacagcaac tggatctcct cttggcgaaa
     2221 ttttagacca ttacagtgat gtcttcaacg gatccattat cctttatctt ttttttcgaa
     2281 ttctccaaat cgaacttgac tggatttttt atttagccat ctggctcaac cttgttgcat
     2341 ttaccttgac ctatttggag cagagtatcc aaaaggaatt gcattttgga aaaataggat
     2401 cactggaagg tgttgtcctc attctcctga tcatctgcag ttttatgaca tctcaaggaa
     2461 aaacattttg gttggaatac accatgctgg acatgccagt ctatgtcttc ttggtattag
     2521 gtttgattat gggtgtgatt tatacagttg taggaagttt aaggcgtttg caacacatac
     2581 ctaagtcttt catccattac ctcttgactg gttctatact ctactttctc tgcatctatt
     2641 atcaaatcaa ctggtatatt gcctttctgg tgatcaatgt ctattcggcc gatctcatcc
     2701 tgaaatccat gaaatctcat cttcttgatg cagagagtcc taaaccggac tttatcgttt
     2761 acatactctt tctgtttctc atctatctgt catttttcga atatgagcaa cagatcggtt
     2821 ttctggtcta tggattggta gtcactgcta aaatcatttg gaacatggta caaatctttt
     2881 ccaaattcag aaattactgg gtttggtgga atctacattg aaacgtagtt cagaattagt
     2941 gacaagtgag agcatgggtt ttatttctca agttcagaca actcatgcat ctcaatccat
     3001 catccaccgt aacattcttt tgtatcccat tttgctactc aagtaatcct ttgcaccgtc
     3061 ttaaaattaa tacactggca agataaaagt atcaagggcg taagagcaag atctttactc
     3121 aacaaaacaa cttcataatt aatttggaaa ttattagatg atttggattt tgatggaagt
     3181 aaccaaggat tatgagagtt tgtcacttct gtttcttaat ttgtcggttt cagaagaacc
     3241 caatctaaat cagaatttgg agtgttttac atcaatcagg aagagtatat ttaagtggaa
     3301 gaacgtgcca accgtgatct ccgcagatgc aacgctcttc aggtagcaga gtaagcttcc
     3361 tcactgcggg gggcacagga agtacatttg aatgttgacc atgcttaccg gtgtacgaca
     3421 cacggttgat caagaattag tcgttcaatt tgcttaaaag ccagtttgcc gtgcgtagca
     3481 gacgggcatc atcatttttt gctcccacaa gctgggtgcc aatcggcatt ccgttttcgc
     3541 cctggaagac gggtatgtta agtgcgggaa caccacagaa ggtccagata gtacagaaag
     3601 acgggtcgcc ggtattatcg aggccaatgg gggcttcacc ggaaacagat ggcgtgagaa
     3661 tggcatcata ttcctggaac agtcctccca gaatgtcatc caaaacagcc tgctgcgaca
     3721 ttgcctggtg gtaagcaacg gctgaatagc tcatcccacg ttcaatctgt aatcgtaagg
     3781 aatcgcttaa cttatctttt gccgtttcgt aatcatgggc aaaactgtaa gcgaggtcag
     3841 cttccataat gattttatga tgatccagca catgtccgaa tgagggagat aactctatct
     3901 cctcaacatt ctcactcagc atttcacata gttcttcatg tgctcctttg cacatttcat
     3961 cggcccggtc ccagacaggc gtttttacga acccgatttt aggtggcaaa ggaggctctt
     4021 cagcggcaac agatattagg gatggtgtgg cgcgaagaaa tgttgtagca gagtcagttt
     4081 cgtcataacc agccaacacc tgtgtcagta aggcaacatc atccgttgta cgggcaaaaa
     4141 cacccaactg gtcgagaggg agtgattgtt gaagaacgcc gctgcgggga atcatcccga
     4201 acgaaggctt gaagccaaac acaccgcaaa acgccgctgg acggatgatg gagccattag
     4261 tctggctgcc cagtgcaatc ggaaccatgt atgatgcgac agccgccgcg gatccgctgg
     4321 atgatccgcc tggtgtgcgg tttgagtcgt gagggttttt tgttttcccc ggtgcatagg
     4381 ttgccagttc cgtagtcacc g
//
LOCUS       DTVD01000262            4358 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7173_length_4358_cov_270.362770, whole genome
            shotgun sequence.
ACCESSION   DTVD01000262 DTVD01000000
VERSION     DTVD01000262.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_4358_cov_270.362770"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(60..980)
                     /locus_tag="EYO68_09795"
     CDS             complement(60..980)
                     /locus_tag="EYO68_09795"
                     /inference="COORDINATES: protein motif:HMM:PRK09653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase"
                     /protein_id="HIC08130.1"
                     /translation="MQLNKYQERIIEIGQTKSAKLVLPERDDPRVSLAKRHLRDLGYE
                     LLEPEDFRGKETQYQEVLEQERFFKKMTDEAKEEYMKDTLNFSMMMVSNGDADGLVAG
                     SITSTSNVLHAAIRIVGVKPKSKWVSSSFFMISPNADTAYTFADCAVIPEPTSDQLAS
                     IAGESAALHYLLTGKEPRVAFLSFSTKGSANHRRVSHVREAISIFAESHPDILHDGEL
                     QVDSALVPAVAAAKAKDSPLSGNSSVLIFPSLEAGNIAYKLTERLAGYTAWGPLLQGL
                     KKPIHDLSRGCSSADIVNVATIAAMQKNRS"
     gene            1191..2075
                     /locus_tag="EYO68_09800"
     CDS             1191..2075
                     /locus_tag="EYO68_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIC08131.1"
                     /translation="MIAKKIQIVEVGPRDGLQNEKIWVETETKIALIEKLADAGLTKI
                     EAASFVSPKWVPQMKDAFEVLSGIERRPGVTYPVLTPNLKGFERALEAGVTEVAVFGA
                     ASEAFSQKNINCSISESVERFRPVLEAAQKNSVRVRGYISCVLGCPYQGEVPLENVVN
                     LAEKMSEMGCYEISLGDTIGIGTPLQAKKMVETVAEKVPVSNLALHFHDTRGQALANI
                     YACLELGVSVIDASVSGLGGCPYAQGASGNVATEDVVYMLHGIGIKTGVDIEKLIETG
                     RFISDVFGRLPQSRVSCA"
     gene            2152..>4358
                     /locus_tag="EYO68_09805"
     CDS             2152..>4358
                     /locus_tag="EYO68_09805"
                     /inference="COORDINATES: protein motif:HMM:PF01315.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC08132.1"
                     /translation="MNTMNPNFGKSGQPMRRLEDDRLLRGQGRFVDNLNFSKQAYLHV
                     VRSYLGHAKIQNIIIDSISGMSGVLAVFTGEDLARDGILPIPMTLPYKRPGGEPALSA
                     PYHALAQERVRFVGQPVAVVVAESKAEALEAGECLEIEYEEIPAVTDLQQAAVPDAPR
                     LCDELPDNIGAAQTEGDAEEVEQAFAQAEHVTRMELVNNRVVGSPLEPRGLLCEAYQN
                     TEKLILHSMHQSATRLHSVLCAVFQLEPKQLQIIVRDIGGGFGTKVAVHPEDILVVYA
                     AKKLKRPVKWTATRTEEFQASVHGRDQWNYAELAFDATGKILALRVHTLANTGAYLTN
                     PALLIPLGLMSKVITSVYHIPAMYLDTQCVLTNTAPIGAYRGAGRPEGIYPMERLMDM
                     AAREMGIEPVSLRERNMIRTESLPYTTLARDVIDSGNFKEVIKRAVRQMDWVGFEDRK
                     AESESRGLLRGRGLACYVEWTGGELTETVRIQAEADGTISLYSGTQGMGQGLETVFSQ
                     LLSEQLEIPLEAVRIVQGDTDQVKGLGSFGSRSLFVGGSALLVGAKDFLEKGKELAAN
                     ELEAAVEDMAYQNGRFEVVGTSIGLGLFELAARQAELSFSTETVKNLEERSWPNGCHI
                     AEVEIDPETGSVKLVRHGSVDDTGNPINPMIVEGQLHGGIAQGAGQVLLEHAVYDSEG
                     QILTGSFMDYAMPRADDFPFFESSTYADAPCLTNPLGAKGVGEIGVVGSIPAIA"
BASE COUNT         1202 a          881 c         1091 g         1184 t
ORIGIN      
        1 tgcaagcaaa atattttgaa attatttcca attcgaggtg tgagactttc aaagttaaat
       61 taacttctat ttttctgcat tgccgcaatt gttgccacat taacaatatc tgctgaagaa
      121 cacccccttg acaaatcgtg gattggtttt ttcaaacctt gtaaaagggg accccacgct
      181 gtatatcccg ccaggcgttc agttaattta taggcaatat ttccggcttc cagagaagga
      241 aaaatcagta cagaagaatt tcctgagagc ggtgaatctt ttgctttagc ggcagctaca
      301 gccggtacca aagctgaatc aacctgaagc tcgccgtcat gtaatatgtc cggatggctt
      361 tctgcaaaaa tgctgattgc ttcccgaaca tggctaacgc ggcgatgatt agcgcttcct
      421 tttgtggaaa aagaaagaaa tgctacacgc ggttccttgc cggtcagcaa gtaatgcagg
      481 gcggcagact cgccggcgat ggaagccagc tgatcacttg ttggttccgg aataaccgcg
      541 caatccgcaa aagtataagc cgtatccgca tttggagaaa tcatgaagaa actgctcgaa
      601 acccattttg attttggttt tactcctaca atccggattg cagcatgaag cacattgctg
      661 gttgaagtga tggaaccagc gaccaagccg tcggcatctc cattgctgac catcatcatg
      721 ctaaaattca gagtgtcttt catatattct tcttttgctt catctgtcat ttttttgaag
      781 aagcgttctt gttctaaaac ttcctggtat tgggtttctt tgcctcgaaa atcttctggt
      841 tccaaaagct cataacccag gtcacgcaga tgtctttttg caagggatac gcgtgggtca
      901 tctctttcag gaagaacaag ttttgctgat tttgtttgtc cgatctcgat aatgcgttcc
      961 tggtacttgt ttagttgcat tacggttgtc cttaaataat taatttagaa ataagttatt
     1021 agccttttga tttgaacgtc attctattgg actctttaaa aaacagcaac atgcattaaa
     1081 atgtctgcac cggaaaaaga tttaagttga cagtaaaaaa tgttagccta acatcccaca
     1141 tgtaaagaac ctgaaaagca tagtcaactt tcttgtataa ctaaagaacc atgatagcaa
     1201 aaaaaatcca gatcgtcgaa gtagggccga gagatggttt gcaaaatgaa aagatatggg
     1261 tcgagactga gactaaaatt gctttgattg agaaacttgc tgatgcagga cttacaaaaa
     1321 ttgaggccgc aagttttgtc agtccaaaat gggttccgca aatgaaggat gcatttgagg
     1381 tgctttcggg aattgagcgc cgtcccggag taacctatcc ggtgctgact ccaaacctga
     1441 aaggttttga aagggcattg gaggcaggtg tgactgaggt tgcggtcttt ggtgcggctt
     1501 ctgaggcatt ctctcaaaaa aatatcaatt gctccatttc tgaatcagtc gagcggttcc
     1561 ggcctgtttt agaagcagca caaaaaaata gtgttcgtgt ccgcggatat atttcctgtg
     1621 ttttgggttg tccctatcaa ggagaggtcc cccttgaaaa cgtggtaaat ttagcggaaa
     1681 aaatgtcgga aatgggctgc tacgaaattt cgcttggtga cacaattgga atcggaacac
     1741 cactgcaggc taaaaaaatg gttgaaacag ttgcagaaaa agtccctgtc tcaaaccttg
     1801 ccctgcattt tcatgatacc cgcggacagg cattggcaaa tatttatgcc tgtctggaac
     1861 ttggcgtgtc tgttatcgac gcatctgttt ccggtttggg cggatgtccc tatgcacagg
     1921 gagcaagcgg gaacgtggcc actgaagacg tggtttatat gcttcatgga ataggtatta
     1981 agacgggtgt tgatatagaa aaactgatcg aaacagggcg cttcatcagt gacgttttcg
     2041 gtcgacttcc tcaaagtcgt gtctcctgtg cgtaaataat atgcaagatt tctctctccg
     2101 ttcgaaatga cacggattta ggactttttt ttcgacggca tcaaagaaag catgaacaca
     2161 atgaatccaa attttggaaa aagcggacaa ccaatgcgac gtcttgaaga tgatcgtttg
     2221 cttcgtgggc aaggcagatt tgtggataac ctgaactttt caaaacaggc atatttgcac
     2281 gttgtacgct cctatctggg ccatgcgaaa attcaaaata ttataataga ttccatcagt
     2341 gggatgtccg gagtgctggc agtttttacc ggagaagatt tagcccgtga tggaatcctt
     2401 cccatcccga tgaccctgcc ttacaaacgg cctggaggcg aacctgctct ttccgctccg
     2461 tatcacgctt tggctcagga aagggtacgt tttgtggggc aacctgtggc agtcgtggtt
     2521 gcggaatcca aagcagaagc actggaagca ggtgagtgtc tggaaattga atatgaagag
     2581 attcctgcag ttacggattt acagcaagct gcagttccgg atgcacccag actctgcgat
     2641 gaacttccgg acaatattgg agcggcgcaa actgaaggtg atgcggaaga agtggagcag
     2701 gcttttgctc aggcagaaca tgttacccga atggaattag ttaacaaccg cgtggtggga
     2761 agtccgcttg aacccagggg tctgctttgt gaggcttacc aaaacacaga gaaactgatt
     2821 ctgcactcaa tgcatcaaag cgcgacacga cttcactcag ttttatgtgc agtttttcaa
     2881 cttgagccca aacaactgca aattattgtc cgggatatcg gcggtgggtt tggaactaaa
     2941 gtagccgttc atccggaaga tattttggtt gtttatgcag caaaaaaatt aaagcgacct
     3001 gtaaaatgga cggcaacacg gactgaggaa tttcaagctt ctgtacatgg acgtgaccaa
     3061 tggaattatg ctgaactggc ctttgatgca acaggaaaaa ttttagccct gagagttcat
     3121 acactggcca ataccggcgc gtatctcact aatccagctt tgctgattcc acttggttta
     3181 atgtccaagg tgattacctc agtctatcat attccggcaa tgtatctgga tactcagtgt
     3241 gttctcacaa acacagctcc aattggcgct tacagaggtg ccggacgtcc ggaaggcatt
     3301 tacccgatgg aacgtttgat ggacatggcg gcccgtgaaa tgggtattga acctgtttca
     3361 ttacgtgaac gcaacatgat tcgaaccgaa agtctcccat atacaacact tgccagggat
     3421 gttattgaca gcggaaactt taaggaggtc atcaaaaggg ctgtcagaca aatggactgg
     3481 gttggttttg aagatcgaaa agctgaatct gaaagccgtg gactgctccg cgggagggga
     3541 ttggcttgtt atgttgaatg gaccggtggt gaattaactg aaactgtgcg gattcaagct
     3601 gaggcagacg ggacgattag cctttattcc ggaacccaag gtatgggcca gggtttggaa
     3661 accgttttca gtcaactact ttcagagcaa ttggaaatcc cattggaggc agttagaatt
     3721 gtacagggag atacggacca ggttaaaggt ctgggaagtt ttggatcacg ctcgttgttt
     3781 gtcggcggaa gcgctttgct ggtgggagcc aaggatttcc tggaaaaagg aaaggaactg
     3841 gctgccaatg aactggaagc agcagttgaa gatatggcct accaaaatgg tcgatttgag
     3901 gttgtgggaa cttctattgg acttggtctt tttgaattgg ctgcaaggca ggcggagctt
     3961 agtttttcaa ctgaaaccgt gaagaatctg gaggaacgaa gttggccaaa cggctgccat
     4021 atagcagaag ttgaaattga tccggaaacc ggaagtgtaa aattggtcag acatggatct
     4081 gttgatgata ccggcaatcc gatcaatcca atgattgtag aaggccagct gcatggaggt
     4141 atagcacaag gtgcgggtca ggttttgctg gaacacgcgg tttacgattc tgaggggcag
     4201 attttgacag gatcttttat ggattatgca atgcccaggg ctgatgattt tccttttttc
     4261 gaatcgtcaa catatgcaga tgcaccttgt ctaaccaatc ctctgggtgc aaaaggagtc
     4321 ggagaaattg gagttgtagg ttcaattccg gcaattgc
//
LOCUS       DTVD01000263            4354 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7188_length_4354_cov_359.742963, whole genome
            shotgun sequence.
ACCESSION   DTVD01000263 DTVD01000000
VERSION     DTVD01000263.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_4354_cov_359.742963"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="EYO68_09810"
     CDS             <1..1115
                     /locus_tag="EYO68_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HIC08133.1"
                     /translation="ELLFVSALGYCMGIAAICGFIGISPEAGAFFAGISVGNLPYRLD
                     IEDKVGPLKLFGQALFFLTLGVEIATIQAKNFSDNMGAIIPLVILVVLIKPFFLIITG
                     YATKLKGRTAFFIGTTLNQSSAVSIIVALLAWKYNLFDDRLFAILITVILLSLLISAM
                     GHAVQPGLYNSFKWLVGVLNRNISALDLENNQQVVLKDHVVLLGFNEIAQEIGEFYEE
                     QLTPERVLLIDCDPEIIKHFEARKDSNVDPVYADPNDPAVWREYKLSEAKVVVSCTGS
                     DLDSDLELAGYIRHTAPDLPFLAVTTSHEDSLKLYESGVRYVVQTDQLASKTFRDIFA
                     EEIDKPASESFVEEGRSHWKDTRSIRDGLGDIFKLV"
     gene            complement(1169..3721)
                     /locus_tag="EYO68_09815"
     CDS             complement(1169..3721)
                     /locus_tag="EYO68_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08134.1"
                     /translation="MKIRLFLIVMIPAFLITSTIGIYLIEYILTGNKDSAFASLFSSL
                     WWTAVTFTTVGYGDMSPETVQGKIFTFFVMAAGLINFSIVVSMVTEKFQEFRSGRDRG
                     LDSIKIKSHILICSDDPTWMKEILSQNQKYVKRDKVVLISPSDEHPLLATSYKKLKWI
                     SGNSFDLNVLRKAAAAKAKIAYVFFKDSSYALMTVLQLETLSDGRIVTQAQYVGREYR
                     KYFEDVGCDHALDPYDLYVPMMLSAFHSQGAPAWINKVINRNHGHQIATKKTDSLHIG
                     KTWLELIKTNKQNHGIMPLAVVINEVVLINPDASFEIPKDSLIMQLEPFEISKVSSVM
                     QFDVTESSPKGDLEKNAIDVMGMDEIGIDGHILISSDNQVFINRCLLEMSQRNQPEKI
                     IVLSDTPLMEEIPDNLDVEWIEGDSNSEKSFLEARSSEAKVALIDHADDGQNLMAVLR
                     LEQATDGEVFTIATYHKEDFDQQLFKVGCDFCLEPEELIAPILSQSALNPGLGTLIEE
                     IISEEPTTQSLHVRQLSREWEEDSWLSTILNLKEKEGELPVGLICCQTHKLLVNPHPE
                     LQVKPGDRLIYIASAPIRAKQNGDVLKPVEDSESTEEDVKPSEEAEKLYRQGLTLFKK
                     DENYEEAYSCFHQAAILNHTRAKYNLGLMNYNGKGVPKNLDESYHWFHEAAKFGNENA
                     RKALKSTRALRDITSNTMEHEIPEFDNDLIGRMPEEQLYWFASAVIAMVMADEHIDLH
                     ERSFLHSAIRIVRDKRKIQELEEYILRWQTPPIGAMDFSTKDRDHILESLLNIAAVDR
                     NFDEREEKLLREIAGLMKVPEQQIENLIELGHKRVEQFRANQLRAPNVRARL"
BASE COUNT         1228 a          911 c          826 g         1389 t
ORIGIN      
        1 cagaactttt gtttgtcagt gcgctgggat actgcatggg tattgcggcc atttgtggtt
       61 ttattggaat ttctccagaa gctggagctt tcttcgctgg aatttctgta gggaacctgc
      121 catacaggct tgacattgaa gacaaagttg gtccactgaa attgtttgga caagcgttat
      181 ttttcctgac actcggagtt gaaatagcaa caattcaggc caaaaacttt tcagacaaca
      241 tgggtgcaat cattccactc gtgattcttg ttgttctcat caaacctttc tttttaataa
      301 ttaccggtta tgcaacaaag ctcaaaggac gcaccgcctt tttcatcggg acgaccttaa
      361 atcagtcatc agcagtttca atcattgtcg cccttttggc ctggaaatat aatctctttg
      421 acgatcgtct ttttgcgatt ttaattacag taattcttct ttcactcctt atctcagcca
      481 tgggtcatgc agttcaaccg ggactatata attctttcaa atggcttgtt ggtgtattaa
      541 accgaaatat ttcagcctta gacctggaaa acaatcagca agtcgtactg aaagaccatg
      601 ttgtactgct gggttttaac gaaatagctc aggagatagg agagttttat gaggagcaac
      661 ttactccaga acgcgtttta ttgatcgact gcgatccaga aattattaaa cattttgagg
      721 cacggaaaga ttccaacgtt gatccggtct atgcggatcc taacgatccg gctgtgtgga
      781 gagaatataa acttagtgag gcaaaagttg ttgtttcctg tactggaagt gacttagatt
      841 ccgatctgga actcgcaggt tacattaggc atacagcacc tgatcttccg tttttagctg
      901 tcacaacctc tcatgaggat tcactgaaac tctacgagag cggggtccgt tatgttgttc
      961 agactgatca gcttgcatca aaaaccttca gagatatctt tgcggaagaa atagacaagc
     1021 ctgcttctga gtcttttgtg gaagaaggca gaagtcactg gaaggatacc cgctcgatca
     1081 gggatggatt gggagatata ttcaagctgg tctgattttt tccggataaa agtatgtacg
     1141 gtttttacgg acttaatact cttttatatc atagtcttgc cctcacattt ggagcacgca
     1201 gttggttcgc cctgaactgt tccacccgct tgtgaccaag ttctatcagg ttttctattt
     1261 gctgctcagg tactttcatc aatcctgcta tttcacgcaa taacttttct tccctctcat
     1321 caaaatttct gtcaaccgca gctatattga gcagactctc gagaatatga tctcgatctt
     1381 ttgtactgaa atccattgct ccaatcggcg gtgtctgcca acgtagaata tactcttcaa
     1441 gctcctgaat ttttctttta tcccgaacta ttcgaatagc tgagtggagg aaagagcgtt
     1501 catgaagatc aatatgctcg tctgccatca ccattgcaat tactgcactg gcaaaccaat
     1561 acagttgttc ttcgggcatt cggcctatca aatcattgtc aaactcagga atttcatgtt
     1621 ccattgtatt tgatgttata tctcgtaaag ccctggtact tttcagtgct ttacgggcat
     1681 tctcattacc gaatttagct gcttcatgga accaatgata tgattcatcc aagttctttg
     1741 gtacaccttt accattataa ttcatcaaac caagattata ttttgctcgt gtatggttca
     1801 gaattgccgc ctgatggaaa caactgtacg cttcctcata attttcgtct tttttgaata
     1861 acgttagtcc ttgacggtat aatttttctg cttcttcaga cggtttaacg tcttcctctg
     1921 tactttctga atcctcaaca ggtttgagaa catctccatt ttgcttagcc ctaatagggg
     1981 ctgaggcaat ataaatcaaa cggtctccag gctttacctg cagctcagga tgaggattaa
     2041 ccaggagttt gtgagtctga cagcagatca gtcctacagg cagttcgccc tctttttcct
     2101 tcaaatttaa aatagttgaa agccaactgt cttcttccca ttcccgactt aattggcgga
     2161 catgcagact ctgggtagta ggttcctccg aaataatttc ttcaatcaat gtacctaatc
     2221 cgggatttaa tgctgactgg gaaaggatgg gcgcaattaa ctcttccggt tccagacaga
     2281 aatcacagcc aaccttgaaa agttgttgat caaagtcttc tttgtgatag gttgcaatgg
     2341 taaacacttc tccatcagtt gcctgttcca gacgcaggac agccatcagg ttttgaccat
     2401 catccgcatg atcaatcagg gcaactttcg cctctgatga tctggcttcc agaaacgatt
     2461 tttcagagtt tgaatctcct tcaatccatt cgacatccaa gttatcagga atttcctcca
     2521 ttaacggagt atctgaaagc acgattatct tctcaggttg atttctttga ctcatttcta
     2581 gcaaacatcg attaatgaac acctgattat cggagctaat taaaatgtgt ccgtcgatac
     2641 ctatttcatc cattcccatc acatcaatag catttttttc aagatcgcct tttggacttg
     2701 actcagtgac atcaaattgc attaccgaac tgacttttga aatttcaaac ggttccagct
     2761 gcattattaa actgtctttc ggaatttcaa atgaagcatc aggattgata agtactactt
     2821 catttataac aactgcaaga ggcataattc catggttctg tttattcgtt tttatcagct
     2881 ccagccaggt tttaccaata tgtaaagaat cagttttttt agtggcaatt tgatgtccat
     2941 ggttgcgatt gatcacttta ttgatccatg ccggagcacc ttgtgaatga aaagctgaca
     3001 gcatcatagg aacgtagaga tcatatggat ccaaagcatg gtcgcaaccg acatcttcaa
     3061 aatacttccg atattcccgg ccgacatatt gtgcctgagt cacaatccta ccgtcactca
     3121 aagtttcaag ctgcaaaact gtcatcaggg cataactgct gtccttaaaa aatacatagg
     3181 caatttttgc ttttgctgct gctgccttcc ttaaaacgtt tagatcaaaa gaattacctg
     3241 agatccattt taattttttg tatgacgtag ccaacagcgg atgttcatca gaaggagaaa
     3301 tcaatacaac cttgtctctc tttacatatt tctgattctg agagagaatt tccttcatcc
     3361 aggttggatc atcagaacat atcaaaatat ggctttttat cttaatggaa tccagtccgc
     3421 gatcacgccc ggaacgaaac tcctgaaact tttctgtcac cattgaaaca acaattgaaa
     3481 aattgattaa acctgccgcc attacaaaaa aagtaaaaat tttcccctga acagtttccg
     3541 gcgacatgtc tccatagccc accgtagtaa atgtaactgc tgtccaccag agagagctga
     3601 atagtgaagc gaaggcgcta tctttatttc cagtcagaat gtactcaatt aaatatattc
     3661 cgattgtaga agtaatcaga aaagccggaa tcatcacaat caaaaataaa cgaattttca
     3721 ttttaaattt tatcagcaga ttattttttt ctcatggatt atgactaata atgtttcagg
     3781 aagcaggtaa ctcggggcca attctcaatc tggatgatta aacttccttg ttcattaaca
     3841 gagcaatatt accaaatttg caagtccttt tgcaaataat acgtttttta catgcaaatt
     3901 ttgggacaca gtaaaaatac tgcttcagag tgttttaatc agttccttaa gaagagtgag
     3961 taaataaaag agtagaataa agtggagaga ctggaattgt ggtcaaatag acagacacaa
     4021 tggaaattct aaagatgtct gaaggcgaat caatgtattt attggtgggc caggcaggac
     4081 ttgaacctgc gaccggacga ttatgagtcc gatagttacg atttatttag actactattt
     4141 attactttca tttagtatta tttatagtaa tatcatacta gtaggtagtt attgagtatt
     4201 attaactact gccaggtgtg acggcttata gacacctata tggacacctt ttccggaatg
     4261 atggacacct atatggacac ctaattatcc ttttttagag agtttttact gcgctttagg
     4321 ttgcgaaata atcaggttct ctaccttttt tcca
//
LOCUS       DTVD01000264           15912 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_718_length_15912_cov_327.111812, whole genome
            shotgun sequence.
ACCESSION   DTVD01000264 DTVD01000000
VERSION     DTVD01000264.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 15912)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15912)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15912
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_15912_cov_327.111812"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="EYO68_09820"
     CDS             <1..1200
                     /locus_tag="EYO68_09820"
                     /inference="COORDINATES: protein motif:HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIC08135.1"
                     /translation="LLLAAERHVVVVGGGFGGATAAKYLRLMDPNLKVTLVEPKRSYF
                     TCPFSNQVLGGMKTMEELTYSYKKLQRKYGINVVHVAAARINAVSKTVLLQDGKNLTF
                     DRIIVAPGIDMRFDQIENYKPEDTDFIPHAWKGRSATLRLLQQLESMPNGGMVLICPP
                     ALPYRCPPAPYERASLVAHYLSQHKPRSKILILDAKEQFPKQALFSAGWKSLYGRMIE
                     WFNGSAGGLILRADAKNMTVETEFGIEKGDVINLIPAQWAGRIARASGLSDESGWCPV
                     DQLTFESTLLPGIHVIGDAAIAGVMPKSGFSANNQAKVTAAAVIALLKGKEPTSYSIS
                     NTCYSFLAPDYAIYVTAEYQLSGRELVKINGSGGVSPLNVDLSVRHSEAVSAQRWYDS
                     ITQDMFG"
     gene            1506..2030
                     /locus_tag="EYO68_09825"
     CDS             1506..2030
                     /locus_tag="EYO68_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIC08136.1"
                     /translation="MDASGNSLELTLAEYAKKPFGNVIFLRHAFAPGFDANGEPDKFE
                     IDDCSTQRNLSAIGRKQAANIGEKFFEKGIRIKTIYSSQWCRCLETAELLKLGEVIPE
                     PSLNSGFKGIFNKEISLLKLKEILKTLENEKEIFLMVTHYGIITAMTGIFVDSGGAVA
                     YNSKTNEYKKILFE"
     gene            complement(2298..4907)
                     /locus_tag="EYO68_09830"
     CDS             complement(2298..4907)
                     /locus_tag="EYO68_09830"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIC08137.1"
                     /translation="MKNPSFFFWPKLALRELLNNRRFSLFFLFNLALGLAGFIALDSF
                     KESLDTHLGRNSQAILGADIALTSYVPFEEKTLNILESQLPDNTLSTRKISLFTMVAT
                     KDQSRLVQVTGIEQDYPFYGKMILKNVGSVDPAKLKQSLDLSGEAWVYPELLYMLELN
                     AGEYIKIGEQSFRIADSVIVDPSSSFSSFGLAPRIYLGYSHMEETALLTKKSRVSHQR
                     LYRVPEGTDLSGLVDKLQKQINKLDGSDSKIRILTHKRAGDNLGRLMGYLNDYMGLVA
                     MIAVFLAGIGAAYLFRNYLVNRFREMSIMMSLGATRQQTYRMVLWQLGLLGTGAALLA
                     IIFSLGILPLLPVLLKQFLPSGFETHVNLSSMLFALVLGGVGSLVFCLPVLSRIRLVQ
                     PLQLFHGNIGSHEVKVKIWRQGLSYLPLLVLSWGLSVWQSHSWVVGSTFVGMLLFAIL
                     LLGSIAWGILILAGKLSQVSGGRTTAGRTMKRLAFRNLERNRTGAISCFLALALGTLL
                     INLIPQIYQGLQEEVSRPEDYRIPSLFLFDIQPDQIDDLQSVLVRENAQLNYLSPMVR
                     ASLEKVNGEAFRSNNDEAPVTREQEREQHIRRHIFNLSYRTELSNAEYIVSGKALAIN
                     YDWESTVPAEVSLEQKFAERMRLKLGDLLTFNVQEIEVEAKVVNLRRVKWNSFQPNFV
                     ILFQTGVLEDAPATFLASLGGLDKTRRLQLQNQIVKEFPNVSVIDVTRMVKRVLKISD
                     QMVLALRLMAYLSILAGLVVVFSIARHEVEGRLWELNLLKVLGARFQDIQKMIQIEFA
                     IMGIFAGFFGVAVSLAISYGLAWWFFENLWKWTWQISLASVIGVTSLSVGVAWLATQR
                     TLRSSPLSLLRCA"
     gene            complement(4904..5566)
                     /locus_tag="EYO68_09835"
     CDS             complement(4904..5566)
                     /locus_tag="EYO68_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08138.1"
                     /translation="MILEIKGLDKSFPQAGHGQIEVLKNLNLEVHEGETVAVVGQSGC
                     GKSTLLSLLAGLDRQTSGSLRIRGMEISEMSEVKLTQFRAENIGIIFQQFYLMPHLTA
                     LENVSLPLEMFGQEDSIERAQEALLQVGLAEREKHFPHQLSGGESQRVAIARAVVIRP
                     SILLADEPTGNLDNATGEQVANLLFDLVKNTGTTLLLVTHNTVLAQRCSRQLTLHFGT
                     FK"
     gene            5812..6168
                     /locus_tag="EYO68_09840"
     CDS             5812..6168
                     /locus_tag="EYO68_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08139.1"
                     /translation="METFNIFLIGAGTGTVFSVISMLVINANNAEQYPVGGFLPALWM
                     VAELGVDSKIVVAGILMMGGMTGYFLILTIWKRRPEALIKTRPQETHVSGDSKLVTED
                     EHSTITVSGKSLAEFL"
     gene            complement(6181..7386)
                     /locus_tag="EYO68_09845"
     CDS             complement(6181..7386)
                     /locus_tag="EYO68_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIC08140.1"
                     /translation="MVMNQLISIQNGRKQSPIFSAAEMENRLAKLRTQMAAENIDAVI
                     FTSIHNINYYNHFVYCSFGRPYGLVVTQDKLKSITANIDGGQPWRRGFGENVIFTDWE
                     KDNYFRAIQQEITNSGRVGIEYDHINLENLKKLQDALSDAELVDISKMTMRQRMVKSA
                     EEIELIRNGAQVADVGGAACVKAIGEGVPEYEVALHSTQAMVREISRRYPESDIMDTW
                     TWFQSGINTDGAHNPVSTRKIQKGDILSLNCFPMIGGYYTALERTLFFDHASDRHLEI
                     WDVNVEVHRRGLELIRPGARCCDIAEELNEIFSKHDLLQYRTFGYGHSFGSLSHYYGR
                     EAGLELREDIETVLEPNMVVSIEPMVMLPETLPGAGGYREHDILVVTEDGAENITAFP
                     FGPEHNIIR"
     gene            complement(7404..8438)
                     /locus_tag="EYO68_09850"
     CDS             complement(7404..8438)
                     /locus_tag="EYO68_09850"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIC08141.1"
                     /translation="MHIENEVKYDFSDVLIKPKRSYYTSRKEVDLERDFKFKWSNLSW
                     KGVPIVAANMDTVGTIGSAEVLSKHKVLTCLHKFHTADELLKISSGIKKNVAITIGVA
                     EGLQTLYDLGEQIKDFNFICIDVANGYTEQFCDFIKLARSEFENKIIIAGNVCTPEMT
                     EQLILSGADIVKVGIGGGSACITRNVAGVGIPQLSAVIECSDAAHGIGGMVMSDGGCT
                     CSGDISKAFGANADFVMLGGLFAGHDENSETITENKISGEKTVEFYGMASSTALEKYY
                     SDNKGKDYKASEGKLVSIPYKGTLENTLLDLLGGVRSTCTYIGAKKIKDISKCTTFVC
                     VNHQINNIYT"
     gene            complement(8441..8902)
                     /locus_tag="EYO68_09855"
     CDS             complement(8441..8902)
                     /locus_tag="EYO68_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphoribosyltransferase"
                     /protein_id="HIC08142.1"
                     /translation="MSKKYYYSWKECLIDIQKIARQVSLSDFNPDVIVGVSRGGLVPG
                     VALSHWFQLPMVPIQTALRDFPTWVKYKPEQAHKKVLIVDDVCDGGETFHKIHEEIKE
                     FCQEPQFASLWWNNECDFTPHFFAREVAKDSENLWIHFPWEFENTQQYITD"
     gene            complement(9046..9825)
                     /locus_tag="EYO68_09860"
     CDS             complement(9046..9825)
                     /locus_tag="EYO68_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3726 domain-containing protein"
                     /protein_id="HIC08143.1"
                     /translation="MGFSVLSGYRGSMKSPEIVLSLNEIMFYFTRAAVGAGLSFGLAE
                     DFGRSSIWIAASGLDPARITSKTLKSLDEAQSSLCASQTEIGAETVLTPSGGKQLSAL
                     FAGPSVCDWILEKKAVSDKTQHFVVEQVDHPFLIAAAVGALKSGNWEICWQDSGGTLC
                     NVLTGQHGNWKTSLEGNEIFQQHSPADVSIKSAESSLFSSKKWIEKTTYSEKNRQVVL
                     ESGVPLYEAWPIIHKLFSRCLVPSTEESHKSGAGAGLVDTD"
     gene            complement(9786..11498)
                     /locus_tag="EYO68_09865"
     CDS             complement(9786..11498)
                     /locus_tag="EYO68_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08144.1"
                     /translation="MKVEMPETTHVVSKGMRSPEKVMRLSRMGSSFQTRLSFMRSLTR
                     RISKEKWKFEKLRFEVDEKGYGTSVYVVHTPERTYSLITFTNEIAPEQRTDRVVAEVW
                     DATFNLFDGIPTEADIKRLSENTPKQEAGRFSPSELVLARANKSLRLFEHVASRLSEG
                     KQPDIELLASVGYLMRTTAVYGSGKFGCADREKISNRSETRGSFQVELLTVYLFRWFT
                     IELVEYVARQRGGRKAVSLNPEISKFLGIGNATGLGMAPFLIKHPVLIHKWVLARETA
                     LSRVVALESHTPESLSLFRKFMFQATQHIAEWNVEDEQQTSRIIKTREDLKQLSNWFA
                     NEENIKQPLIWERILRFAEANFSLEGQELTVSLLLESHGKLVDELADDMQTSTGIELE
                     PSMSLNQLGELLQKSFNWALEIDFDDPEKQRRFWYYSEEKLEPRFGDRYADPGAEQEM
                     PLAVGRDVFLLNKKIKSVTDDISVGTFVQNHPEFRNIVRRVQTVVRFPYAEIRDNIVD
                     AEMRPIDLLRFKLAFFGASKFDPKSELWTRITLFQGAPLPDQFVGNDSDEWAFPFCPD
                     IVAA"
     gene            complement(11507..12496)
                     /locus_tag="EYO68_09870"
     CDS             complement(11507..12496)
                     /locus_tag="EYO68_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HIC08145.1"
                     /translation="MKTPKLPLIIDGLQYNNWSEDIFREMNEGGVAAVHVTICYHEDF
                     QEMVENVIAWNRLFKLHSELIFQGRCAEDVLKAQNEGRTAIIFGFQNCSPIEDDIGLV
                     EICHQLGVRFMQLSYNNQSLLATGCYEDDDSGITRMGRQVIKEMNRVGLVVDMSHSAR
                     RSTLEAIEISARPIAITHANPFFWCEALRNKTDDVLKAIGKSGGMLGFSVYPHHLKDK
                     SACLLEDFCGMIARTAEMIGVERIGIGSDLCQNQPDSVVEWMRNGTWSNERDFGEGSA
                     ELAGFPEQPEWFRDNRDFENIFSCLRKTGFSENEVERIAGLNWLEFFEKSFGP"
     gene            complement(12532..13365)
                     /locus_tag="EYO68_09875"
     CDS             complement(12532..13365)
                     /locus_tag="EYO68_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HIC08146.1"
                     /translation="MAKILTEEQIQQFHQNGFVSPIRVMSEEEAYSIKAKIEEAEKKF
                     PEDFNPEKRNNLHLTFMVLDELAHNNVIVDTVEDLIGPDISLWSSVMFIKDASTNHFV
                     SWHQDATYMGMNSSDFLTPWFALTPSTIEMGCMSMIPGSHKQSIRKHEDTFAENNILT
                     RGQVVKNVDKSKAVDLILKPGEMSIHHGAAIHGSKPNKSKQRRIGFSLQSYMTPSVEQ
                     IVGKNIWMHIRGKKRQDRDGMRLYRPQYDMDIRSVSQRKFADENYSNILYNGSKIKRK
                     Y"
     gene            complement(13579..14592)
                     /locus_tag="EYO68_09880"
     CDS             complement(13579..14592)
                     /locus_tag="EYO68_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-alpha-acetyl diaminobutyric acid deacetylase
                     DoeB"
                     /protein_id="HIC08147.1"
                     /translation="MNHTEPKVSATIDLLADGKHHGHLIIPHSRNESGWGAVHLPIVS
                     IRNGAGQTMLLTGGNHGDEYEGPIALMKLARTLEAGGIRGQVIIIPALNFPAVLTGSR
                     LSPIDGVNMNRAFPGRRDGSVSLMIAHFVHHKILPLADVVLDIHSGGKTMMFSPFACY
                     HRIPDAEVMERAKQAMLAFGAPISLELVELDSEGMLDTEVEEMGKIFVGCELGGGGTS
                     TPETVSITEIGVRNLLAHFEIIDEKPLSCEDRGLPPTRLMHMPDSDCYLISNDAGIYE
                     TLIDLDETVKKGDAVGQVHFPEKLELQPVVYCAGRDGTLIGRTHKALVGIGDFLGMIA
                     TEM"
     gene            complement(14713..15888)
                     /locus_tag="EYO68_09885"
     CDS             complement(14713..15888)
                     /locus_tag="EYO68_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIC08148.1"
                     /translation="MMDSPVRGFPVSEFEARLKRVQKLMAAEDLAGILLMTEPEVRYF
                     SGFHTQFWQSPTRPWFLFIPADKKPIAVIPEIGAALMKTTWIEDIRTWGAPWPKDDGI
                     TLLLDLLEPLSIRGSRLGIMKGHETSLRMPLGDYESLMSGLPGMELVDATDILRSLRM
                     VKSEAEIEKISHICNIASRTFERANEIFYKGQPLAEAFRAFRMECLAQGADDVPYLAG
                     GADQGGYRDIISPPSQRPLQSGDILMLDTGATFDGYFCDFDRNFSIEQSDDLSHQTYD
                     ILWRATEAGMTAARPGTTCRELFHSMQSVIAEIDVQGGEVGRYGHGLGMQLTEWPSHA
                     DFDETVLEENMVITLEPSLNYGDGLIMVHEENIVVQNGAPRLLSMRAAPELPIIGGR"
BASE COUNT         4575 a         3694 c         3034 g         4609 t
ORIGIN      
        1 ctgctattgg cagcagagag acatgtcgtc gtcgtaggtg gtggttttgg cggtgccacg
       61 gcagcaaagt atttgcgcct gatggacccc aatctcaaag tgacactggt tgagcccaaa
      121 cgcagctact ttacttgtcc tttcagcaat caggtgctgg gtggaatgaa gacgatggaa
      181 gaattgacat atagctataa aaaactccaa cgcaagtatg gaatcaatgt ggttcatgtt
      241 gctgccgcta gaataaatgc agtgtctaaa acagttcttc tgcaagatgg aaaaaactta
      301 actttcgacc ggatcattgt ggcacctgga atagatatgc gttttgatca gattgagaac
      361 tacaaacctg aagacacaga tttcataccg catgcctgga agggtcgttc cgccactctc
      421 cgtctgctcc aacaacttga atccatgcca aatggtggaa tggtgttaat ctgtccccct
      481 gcccttccct accgctgtcc tccggcacct tatgaacgtg ccagtttagt tgcccattac
      541 ttgagtcagc ataaaccacg ttctaaaatc ctaattcttg atgccaaaga acagttcccc
      601 aaacaggccc ttttttctgc aggatggaag agtctgtatg ggcggatgat agagtggttc
      661 aacggctctg ccggtgggct gattttgcgg gctgacgcaa aaaatatgac cgttgagaca
      721 gagttcggca tagaaaaagg tgatgtgatt aatttaattc ctgcacagtg ggcaggtcgt
      781 attgctcgag cttccggatt atctgacgag tctggatggt gtccggtgga tcaactcact
      841 tttgaatcaa ctctgctccc ggggattcat gtaatagggg atgccgcaat cgctggagtt
      901 atgccaaaat ccggattttc ggccaacaac caggcaaaag taacagcagc agcagtcatt
      961 gcactgctta aggggaaaga acctacatct tactctattt ccaatacctg ctacagtttt
     1021 ctagctccag actatgcaat ttatgtaaca gctgaatatc aactgtccgg aagagagtta
     1081 gtcaaaatta atggttccgg gggtgtcagt ccactgaatg tcgacctgtc agtgcgtcac
     1141 tccgaagctg tttctgccca gagatggtac gacagtataa cacaggatat gtttggataa
     1201 tcagttctga cgacctcata tccttaacta aactgataat caaacagtga gcaattacta
     1261 attcctttta acagcgcaat tcatcccttc agtaaatagg gcgcttttac aaaaaatagg
     1321 gcaaaatgca atattttatt gatcgcttaa gttatcaaaa taaatataat ttactgttgt
     1381 ttataaaatc agttgttcaa cttgacacat ccaatctgaa gtttaaatta cacacaatat
     1441 ctgcagttaa atttagcatg ttaatcaaaa ggattttttg tttctgcatt ttatttttat
     1501 gttttatgga cgcatcagga aattctttgg aattaacact tgcagaatat gcaaaaaaac
     1561 catttggtaa cgtaatattt cttcgccatg cttttgctcc gggatttgat gctaatgggg
     1621 aacctgataa atttgaaatt gatgattgct caactcaacg taacctgagt gcaatcggta
     1681 ggaaacaagc agcaaacata ggtgaaaaat tttttgaaaa aggaattaga atcaaaacaa
     1741 tttattcaag ccaatggtgc cgttgtctgg aaacagcaga acttctcaaa cttggtgagg
     1801 ttatcccaga accatcatta aattcaggtt tcaaaggaat atttaataaa gaaatctcac
     1861 ttttaaaact aaaagaaatt ttaaaaacat tggaaaatga gaaagagatt tttttgatgg
     1921 ttactcatta tggtataatt acagccatga ccggaatttt tgtagatagt ggtggtgctg
     1981 ttgcttacaa ttcaaaaact aatgaatata agaaaatttt atttgaataa tatgatcaat
     2041 tatttcattt aatttttttt attattctat taacagggtt taatatcctt tcaatatagt
     2101 agagactgac gaaacttgtt actagtcaac ttttttgtaa ctatttttta aaaacattta
     2161 taaatttttc aataaataat cttaaagaca aactgtaaat aactgtttct aaaagagaat
     2221 attcctttta atctcaagca caccattcaa aatactttaa attgagagca gcaaagtttt
     2281 ttgccgttca aaaataatca ggcacacctc aacaaagaaa gcggagaact tctcagagtt
     2341 cgctgagtag ccagccaagc tactccaaca cttaaagatg taactccgat aacgctggcc
     2401 aaactaatct gccaggtcca tttccagagg ttttcaaaaa accaccaggc aagtccatat
     2461 gaaattgcca aactgactgc aactccgaaa aatcctgcaa agattcccat tattgcaaat
     2521 tcaatctgaa tcatcttttg gatgtcttga aatcgtgcac caagtacctt aagcaggttc
     2581 aattcccaaa gccggccttc cacttcgtga cgggcaatcg aaaaaaccac caccaggcca
     2641 gccaaaatgg acagataggc cattaacctg agagctaaaa ccatttgatc tgaaattttc
     2701 agtacacgtt taaccattct ggttacatca atcacagaaa cattgggaaa ttcttttaca
     2761 atctggtttt ggagttgcag cctgcgagtt ttatccaagc cacccagcga agctaaaaac
     2821 gtagctggcg catcttccag tacaccagtt tgaaaaagga tgacgaaatt tggctgaaag
     2881 ctgttccatt tcacccttct caggtttacg acttttgctt caacttcaat ttcctgaacg
     2941 ttaaatgtca acagatcacc caactttaat ctcatccttt cagcaaactt ttgttcaagg
     3001 gaaacttccg ccggcacagt agattcccaa tcataattta tcgccaatgc ttttcctgac
     3061 acaatatatt ctgcatttga cagctctgta cgatatgaca aattaaaaat atgcctccta
     3121 atgtgctgct cacgttcctg ttcacgagta acgggtgcct catcattgtt tgaccgaaat
     3181 gcctcaccat ttactttttc cagacttgct ctgaccatcg gtgagaggta gttcaactgc
     3241 gcattttccc ggactaaaac actttgcagg tcatcaattt gatccggctg aatatcaaac
     3301 agaaacaaac ttggtatacg atagtcttca ggacgtgaaa cttcttcctg caaaccctga
     3361 taaatttgtg gaatcaaatt tatcagcaat gttcctaaag caagtgccag aaaacatgaa
     3421 atagcacctg tacggttacg ttcaagattc cggaaagcaa gacgtttcat agttcttcct
     3481 gcagtagtcc ttccgccgga aacctgacta agtttccctg caagtatcaa tatcccccag
     3541 gctatgctac ccagcagtaa aatcgcaaac agcagcattc ccacaaaagt actgcccacc
     3601 acccatgagt gtgactgcca gacagacaag ccccaggaca ataccaacaa cggcaggtag
     3661 ctcagacctt gacgccagat cttaacttta acctcatgac tgcctatatt gccatgaaaa
     3721 agctgaagcg gctgtaccag gcgaattctg gacaaaactg gtaaacagaa gaccaggctt
     3781 ccaactccac ccagaactaa cgcaaataac atactgctca aatttacgtg tgtttcgaaa
     3841 ccgcttggca gaaattgttt cagcagtact ggtaaaagag gtaaaattcc taatgaaaat
     3901 attatcgcaa gcagcgcagc acctgttccc aacaatccca gttgccataa taccatgcgg
     3961 taagtttgct gacgggttgc acccaggctc atcattattg acatttcccg aaatcggttg
     4021 acgagataat tacgaaaaag atatgctgca ccaattcctg ccagaaaaac tgcaatcatg
     4081 gccaccaaac ccatataatc gtttaagtag cccatcaggc gtcccagatt gtcaccggct
     4141 cttttatgtg tcagtattct tatttttgaa tctgaaccgt caagtttgtt tatttgtttt
     4201 tgcagtttat ccactaaacc agacaagtca gtaccttccg gaactcgata caaacgctga
     4261 tggcttactc tgcttttttt tgtcaaaaga gcagtttcct ccatgtggga gtaaccgaga
     4321 taaatacggg gagcaagtcc gaatgatgaa aatgaactgc ttgggtcaac tataactgag
     4381 tctgcaatgc ggaaactttg ctctccaatt ttgatatact cgcctgcatt aagttccaac
     4441 atgtacagca gttccggata aacccaggct tctccagaca aatccaagct ttgcttcagt
     4501 ttcgcaggat ccactgaacc gacatttttc aaaatcattt ttccataaaa cggatagtcc
     4561 tgttctatac ctgtgacctg aaccagacgt gactgatcct tagttgccac catggtgaac
     4621 aaacttattt ttcgtgtgga taatgtatta tcaggtaact gagattccag gatattcaaa
     4681 gttttttctt caaacggaac atagctggtc aaggcaatat cagctccgag aatggcttgt
     4741 gagttccgac ccaaatgagt atccagagat tctttgaagg agtctaaagc aataaatccg
     4801 gccagaccga gggccagatt aaaaaggaaa aagagactga agcgccgatt attcaataac
     4861 tcccgcaatg ccagttttgg ccagaaaaag aacgacggat ttttcattta aaggttccga
     4921 aatgaagcgt caactgcctt gaacatcgct gtgccaatac tgtgttatgt gttacaagca
     4981 gcagggtcgt ccccgtattt ttcaccagat cgaaaagcaa atttgctacc tgttctcctg
     5041 ttgcattgtc caaatttcct gtaggttcgt ctgcaagcag aattgaagga cggataacaa
     5101 ctgctctggc aatggccacc ctttgtgatt ctccaccact gagttgatgt ggaaaatgtt
     5161 tttcccgctc cgcaagtccc acttgcagca gagcctcttg tgcgcgttct atactatctt
     5221 cttgtccaaa catttccaaa ggcaggctga cattttccaa tgcagtcagg tgcggcatta
     5281 aataaaactg ctgaaaaatt atcccgatgt tttcagcacg aaattgagtc agtttcacct
     5341 cactcatttc agaaatttcc ataccacgta tacgcagtga tccggaagtt tgacggtcaa
     5401 gtccagccag cagagacaat aacgtggatt ttccgcatcc ggattgtcca acaaccgcta
     5461 ccgtctctcc ttcatgaact tcaagattta agtttttcag aacttcgatt tggccatgac
     5521 cggcctgtgg aaaagactta tcaagtccct taatttcaag aatcataaat ttgttgtgta
     5581 aaaatatgct gttttttgat gataaaatac tgtttcagta tacaaaatac tttgactgaa
     5641 atcaaattaa gtcctgctgt atttagtatc attttgtatc gttagagcta caactgttaa
     5701 aattaaacaa cttgaaattt tacttttgca gaaaattgtt ttatcttgaa tttattgtag
     5761 ttgttgttat ttctatatat cagtttttaa acacttttat tttgaaattt tatggaaacc
     5821 tttaacattt ttttgattgg tgcaggaaca ggtacagttt tttcagtaat cagtatgcta
     5881 gtgatcaatg caaataatgc tgagcaatac cctgtaggcg gattcctgcc ggcactgtgg
     5941 atggtagccg agttgggagt ggactccaaa attgttgtgg ccggaattct aatgatgggt
     6001 ggaatgacag gctatttcct gatcctgaca atttggaaaa gacgtcctga agcattgata
     6061 aaaacacgcc ctcaagaaac tcatgtttcc ggcgattcaa aattagtgac tgaagatgaa
     6121 cactcaacta taaccgtcag cggaaagtcc ctagcagaat ttctctagaa actgtttgaa
     6181 ttagcggata atattgtgtt ccggaccaaa agggaaagcc gtgatatttt ctgcaccatc
     6241 ttccgtcaca acaagaatat catgttcgcg gtatcctccg gctccaggca aagtctccgg
     6301 aagcatcacc ataggttcta ttgagacgac catgttcggt tcgagcactg tttcaatatc
     6361 ttcacgcagt tcaagcccgg cttcacgtcc ataatagtga gaaagacttc cgaaggagtg
     6421 tccatatcca aaggttcggt attgcagcaa atcatgtttg ctgaatatct cattcagttc
     6481 ctctgcaata tcacaacagc gcgctcccgg acgaatcagt tccaagccac gccgatggac
     6541 ctcgacattg acatcccata tttccaaatg acggtctgat gcatgatcaa aaaataaagt
     6601 cctctctaaa gccgtgtaat agcctccaat catcggaaaa cagttaaggc taagaatatc
     6661 ccctttttgt attttacgtg tactgacggg attgtgagca ccatccgtgt tgattccgga
     6721 ctgaaaccag gtccaggtat ccataatgtc actttcagga tagcgtctgg aaatttcgcg
     6781 aaccatcgct tgagtggagt gcaaagctac ttcgtattcc ggaaccccct ccccaatcgc
     6841 cttcacacag gccgctcctc cgacatccgc tacctgtgca ccgttgcgga tcaattctat
     6901 ttcttctgca gattttacca tccgctgccg catggtcatt tttgaaatgt ccacaagttc
     6961 tgcatcagac aaagcgtcct gaagtttttt caggttttcc agattgatgt gatcatactc
     7021 tatcccaact cgtcctgaat tcgttatttc ctgttggata gctctgaaat aattatcttt
     7081 ttcccagtcg gtaaaaataa cattttcccc aaacccccta cgccatggct gtcctccgtc
     7141 aatatttgca gtaattgact taagcttatc ctgtgtcacc accaatccgt atggtcgtcc
     7201 gaaagagcag taaacgaaat gattgtaata attgatatta tgaattgaag taaaaataac
     7261 tgcatctata ttttctgcag ccatctgagt ccgcaattta gccaaacggt tttccatttc
     7321 agctgcagaa aatattggag attgtttcct gccgttttga attgaaataa gttgattcat
     7381 aaccaatctt tttctgtcag atcctatgta taaatattat ttatctggtg attaacacaa
     7441 acaaaagtag tacattttga gatgtccttg attttttttg caccaatata tgtacatgta
     7501 ctccggacac ctcctaaaag atcaagtaaa gtgttttcca atgtcccctt gtaaggtatt
     7561 gatacaagtt tgccttcact cgccttataa tccttccctt tgttatcact gtaatatttt
     7621 tccaacgcag tactgctggc cattccgtaa aactctactg ttttttctcc tgaaatttta
     7681 ttttccgtaa tagtctcact attctcatca tgaccggcaa acaagccgcc cagcatcaca
     7741 aaatcggcat ttgctccaaa ggctttacta atgtcaccgg aacaagtaca accgccgtct
     7801 gacatgacca tcccgccaat tccgtgcgct gcatcagaac actcgataac tgcacttaac
     7861 tgaggaatcc cgactccggc cacatttctg gtaatacatg cagagccccc tccaatccca
     7921 actttaacga tatctgcacc tgacaaaata agctgttccg tcatttccgg cgtacaaaca
     7981 ttgccggcaa taattatttt gttttcaaac tcagagcgtg ccagtttaat aaaatcacaa
     8041 aactgttcgg tgtaaccgtt ggctacatca atacagataa aattaaaatc ttttatttgc
     8101 tcaccaagat catacagtgt ctgcaaacct tctgcgactc ctatagtaat tgcgacattt
     8161 ttctttatac cactggatat tttaagtaat tcatccgcag tatgaaattt atgcaaacag
     8221 gtcagtactt tgtgtttgct taggacttca gccgaaccga ttgttcctac agtatccata
     8281 tttgccgcaa caatcgggac tcctttccag gacagattgg accacttaaa tttgaaatcc
     8341 ctttccagat caacctcttt tcgggaagta tagtagctgc gcttcggttt aatcagcacg
     8401 tctgaaaaat catatttgac ttcattttct atatgcatat ttaatccgtt atgtattgtt
     8461 gagtattttc aaattcccag gggaaatgaa tccagaggtt ttcactgtct ttggctactt
     8521 ctctggcgaa aaaatgcggc gtaaaatcac attcattgtt ccaccagagg gaggcaaact
     8581 gtggttcctg gcaaaattct ttgatttcct catgaatttt gtggaatgtt tcgcctccat
     8641 cacaaacatc atccacaatc agtacttttt tgtgtgcttg ctcaggttta tattttaccc
     8701 aggttggaaa atctctcagt gctgtttgaa ttggaaccat cggcaattga aaccagtgac
     8761 tgagggcaac accaggaaca agcccgcctc ttgaaactcc aacaataaca tccgggttaa
     8821 agtcggacaa agaaacctgc cttgcaattt tctgaatatc gatcaggcat tccttccatg
     8881 aataatagta ttttttggac atctgatcta gtaagtacct atataatgaa aaattaaggg
     8941 aaatttaaga agtcataaca aactgtctgc atatcaatca taactaaagc aattactaca
     9001 gtaaagacca tagagctgaa agttatgttt tagtgcaatt agtttttaat ccgtatccac
     9061 caaaccggct ccggcacctg acttgtgcga ctcctcggtg gaaggaacca ggcagcgaga
     9121 aaataattta tggattatcg gccaggcttc gtataatggt actcctgatt ccaaaacaac
     9181 ttgcctgttt ttctcagaat atgtggtttt ctcaatccac tttttagaac tgaacaaact
     9241 actttccgca gatttgatcg acacatccgc tggactgtgc tgttgaaaaa tctcatttcc
     9301 ttccaatgaa gttttccagt ttccatgctg acctgtcaaa acattacaga gagtgcctcc
     9361 ggaatcctgc cagcaaatct cccaatttcc ggattttaaa gctccaacag ccgctgctat
     9421 cagaaaaggg tgatcaactt gttctacgac aaaatgctga gttttgtctg aaaccgcttt
     9481 tttttctaaa atccagtcac aaactgaagg tccagcaaaa agtgctgaaa gttgttttcc
     9541 tccggaaggc gtcagtacag tttcagcgcc aatctcagtt tgacttgcac agagactgct
     9601 ttgagcttca tcaagagact ttagagtttt tgaagtaatt ctggctggat caagaccact
     9661 ggctgcaatc caaatggagg aacgcccaaa atcctctgcc aaaccaaaag acagtcctgc
     9721 tcctaccgct gctctggtaa agtaaaacat tatttcatta agagatagga cgatttcagg
     9781 agatttcatg ctgccacgat atccggacag aacggaaaag cccattcgtc tgaatcgtta
     9841 ccaacaaatt gatctggaag aggagcaccc tgaaataagg taatacgtgt ccataactct
     9901 gattttggat caaatttgga ggctccaaaa aaagctagtt taaaacgcaa gagatcgatg
     9961 ggacgcatct cggcgtccac aatgttatcc cttatttctg catacggaaa acgtacaact
    10021 gtctgcactc tgcggacaat attacggaat tccggatgat tttgaacaaa cgtgcctaca
    10081 gaaatatcat cggtaacgct cttaattttt ttgttaagca ggaaaacatc acgtcctaca
    10141 gcaagcggca tttcctgttc tgcccccgga tcagcatatc tgtctccaaa acgtggttcc
    10201 agcttttctt cggaatagta ccaaaaacga cgttgttttt ccggatcatc gaagtcaatt
    10261 tccaacgccc agttaaatga tttctgcagc aattctccca actgattgag gctcattgac
    10321 ggctcaagtt ctatcccagt ggaagtttgc atatcatctg ctaactcatc tacaagttta
    10381 ccgtgcgatt ccagtaatag tgagactgtc aactcctgac cttcaagcga aaagttggct
    10441 tctgcaaagc gtaatatcct ctcccagata agtggctgtt taatattttc ttcattcgca
    10501 aaccagttag acaactgctt aaggtcttca cgtgttttta ttattcgaga agtttgttgt
    10561 tcatcctcaa cattccactc agcaatgtgt tgagttgcct gaaacataaa tttcctaaat
    10621 aaggacaagg attccggagt atgtgattca agagcaacaa ctctggaaag ggcagtttcg
    10681 cgcgctaaaa cccatttatg gatcaacact ggatgtttta tcagaaacgg agccataccc
    10741 aaaccggtgg catttcctat tccgagaaat ttactgattt ccggatttaa actgacagcc
    10801 tttcgtccac cacgttgtct tgcaacatat tcaaccagtt ctatagtgaa ccagcggaaa
    10861 agataaaccg tgagcaattc tacctgaaaa gatccgcgcg tttccgagcg attggatatt
    10921 ttttccctgt cagcgcaacc gaattttcca ctgccataaa ccgctgtagt acgcatcaga
    10981 taaccgacag aagccaatag ctcaatatcg ggctgctttc cctcagacaa cctcgaagcc
    11041 acatgttcaa acaggcgtaa acttttattg gcacgcgcca gcaccaattc actggggctg
    11101 aatcgaccag cctcttgttt tggagtgttt tcagagagtc tttttatgtc cgcctcagtt
    11161 ggaatgccat cgaaaagatt gaaggttgcg tcccatacct cagctaccac acggtcagtg
    11221 cgttgctccg gggcaatttc atttgtaaac gttatcagtg aatatgtacg ctccggagtg
    11281 tggacaacat aaaccgaagt tccataacct ttctcatcga cttcaaaacg caatttctca
    11341 aatttccatt tttcttttga gattctacgg gtcagactgc gcataaaact aaggcgcgtc
    11401 tgaaaagagg aacccattct ggacaaacgc attacctttt caggagaacg cattcctttt
    11461 gaaacaacat gggttgtctc aggcatctcg actttcatcg tttcttttac ggaccaaaag
    11521 atttttcaaa aaattcaagc cagttaagtc cggcaattcg ctctacttca ttttcagaaa
    11581 aacctgtttt tctcaaacaa ctgaaaatat tttcaaaatc acgattgtcg cgaaaccatt
    11641 ccggctgttc aggaaaaccg gccaactccg cagacccttc accgaaatcc ctttcgttgc
    11701 tccaggtacc gttacgcatc cactcaacca cgctgtcagg ctggttttga catagatcac
    11761 tgccaatgcc gatcctttca actcctatca tttccgcagt cctcgcaatc atgccgcaga
    11821 aatcttccag caaacacgct gatttatctt tcaaatgatg cggatacact gaaaaaccga
    11881 gcattccacc acttttccct atagctttta agacgtcatc agtcttgttt cgcagcgcct
    11941 cacaccagaa aaaagggtta gcatgagtaa tggcaattgg tcgtgctgaa atttcgatag
    12001 cctcaagagt agaacgacgc gcagaatggc tcatgtcaac aaccagtcca accctgttca
    12061 tttccttgat cacttgacgc cccatcctgg ttattccgga atcgtcatct tcataacagc
    12121 ctgttgccag cagactttga ttgttgtaag aaagctgcat aaaacgtacg ccaagttgat
    12181 gacaaatttc aactaaacca atatcatcct caattggaga gcaattctgg aaaccaaaga
    12241 taattgctgt tcgcccctca ttctgagctt tcagaacatc ctcagcgcag cgtccctgaa
    12301 atatcaattc ggaatgcagc ttaaaaagtc ggttccaggc gatcacattc tctaccatct
    12361 cctgaaaatc ctcatggtaa catattgtca catgcacagc cgctactccg ccttcgttca
    12421 tctctcggaa aatatcctct gaccagttgt tgtactggag tccgtcaatg attaagggga
    12481 gtttaggagt tttcatggat ttattacttg tttgagaaca aaacaaaaat actaatattt
    12541 acgctttatc ttagatccat tatatagaat gtttgagtaa ttttcatcag caaattttcg
    12601 ctgggaaact gagcggatat ccatatcata ttgaggccga tacaatctca taccatcacg
    12661 atcttgtctt ttcttgccgc gtatgtgcat ccaaatattt ttgccaacaa tttgttctac
    12721 actgggtgtc atatatgatt gtagagaaaa gcctattctt ctttgcttgc ttttgtttgg
    12781 ctttgaacca tgaatggctg caccatggtg aattgacatt tctccaggtt tcaggatcaa
    12841 atcaacagcc tttgatttat caacattttt gacaacttgc ccccgtgtta aaatgttgtt
    12901 ttcagcaaat gtgtcttcat gtttgcgaat actttgcttg tgactgcctg ggatcatcga
    12961 catacacccc atttctatag tgctcggagt taatgcaaac cagggcgtga ggaaatcact
    13021 actattcatt cccatatagg ttgcatcctg atgccagctc acaaagtgat tagtcgaagc
    13081 atctttgata aacataacag aggaccataa ggaaatgtcc gggcctatca aatcttctac
    13141 tgtgtccaca ataacattgt tgtgagccaa ttcatccagt accataaaag tgaggtgtaa
    13201 attattgcgc ttttctggat tgaaatcttc aggaaacttt ttttcagctt cttcaatttt
    13261 tgcctttatt gagtacgctt cttcttctga catgacgcga atcggcgaga caaaaccatt
    13321 ttgatggaat tgctgaattt gttcttctgt taagattttt gccattattt tactactttc
    13381 aatttagttt aactttgcgg ccacaaagtt acgccagctt tcgaaaatat ttgtccgaaa
    13441 cccctcaggt catcttcgat tcaggcacgg cgtagacaac tctacatttg ttcctgattg
    13501 ctgacgaaaa caggtttgca taattttatt caagttgttc aaaaatttcg atagaacgca
    13561 aaacactaca tcgctagctc acatctcggt ggcaatcatc cccaggaaat caccgattcc
    13621 gaccagtgcc ttgtgggtac gtccgatcaa ggttccgtca cgaccagcac agtaaacgac
    13681 aggttgaagt tccagttttt ccggaaaatg gacttggcca acggcatcgc ccttcttgac
    13741 ggtctcatcc agatcgatca acgtctcata aataccggca tcgttgctga ttaaatagca
    13801 gtctgaatcc ggcatgtgca tcaatctggt cggcggaagt cccctatcct cgcagcttag
    13861 aggcttttcg tcgatgatct caaaatgtgc cagcaagttc ctaactccga tttcagtgat
    13921 cgacactgtt tccggtgtag acgttcctcc gccaccgagt tcacagccga cgaagatctt
    13981 gcccatctcc tcaacctctg tatcgagcat gccttcggag tccagttcaa caagctccag
    14041 actgatagga gctccgaaag ccagcatcgc ctgtttcgcc cgttccatga cttcggcatc
    14101 aggaatccgg tgataacagg cgaaaggaga aaacatcata gtctttccac cggaatggat
    14161 atcgagaacg acatcggcca atggcaggat tttatggtga acgaaatgag cgatcatcaa
    14221 ggaaactgag ccgtcacgtc tacccggaaa agccctgttc atgttaactc cgtcaatcgg
    14281 tgacagccta ctaccggtca aaacggcggg aaagttcagt gctggaatga tgatcacctg
    14341 acctcttatt cccccggctt cgagagttct agccagtttc atcagggcga tcggtccctc
    14401 gtattcgtct ccatgattgc cgccggttag aagcatcgtc tgccctgcac cgttccggat
    14461 cgaaacgatc ggtagatgca ccgcccccca gccagactcg ttgcgggaat gtgggataat
    14521 taaatgtccg tggtgcttgc cgtctgccaa taaatctatt gttgcgctta ctttcggttc
    14581 tgtatgattc atattaccat tctcctataa aatattatta gatacactcc agatggcaca
    14641 agtttaattt aggactcaag ttcctgccgc atgggacata tgacaccatt tgttttaatg
    14701 tctaaataat tttcatctcc caccgataat tggcagttct ggtgcagcac gcatagataa
    14761 gagtcgtgga gcaccatttt gcacaacgat gttttcttca tgcaccataa taagcccatc
    14821 tccataattt agtgacggct ctagtgtgat taccatattt tcctctaaaa cagtttcatc
    14881 aaaatccgca tgagaaggcc attccgtgag ttgcattcca agaccatggc catatcggcc
    14941 aacctcacct ccctgaacat caatttcagc aataacactt tgcatcgaat gaaataactc
    15001 gcgacaggta gttcccggcc ttgcggcggt catacccgct tctgttgcac gccacagtat
    15061 atcataggtt tggtgcgaca agtcgtcgga ttgctctatg gaaaaattac gatcaaagtc
    15121 acaaaaataa ccgtcaaatg ttgcgcctgt gtctaacatc aaaatatctc ctgactgtaa
    15181 cggcctctgt gaaggtggtg aaattatatc gcgataaccg ccttgatctg ctcctcctgc
    15241 caaatatggg acatcatccg ccccttgtgc caggcattcc atgcggaaag cgcgaaaggc
    15301 ttcagcaagc ggttgacctt tgtaaaatat ctcgtttgca cgctcaaagg tccgcgaggc
    15361 aatgttgcaa atatgtgaaa ttttctcaat ttctgcttct gatttcacca ttcgcaaact
    15421 gcgcagaata tctgtagcat caaccagctc cattcccggc aatccagaca tcaagctttc
    15481 gtaatctcca agaggcattc gcaaacttgt ttcgtggcct ttcatgatgc ccagtcgtga
    15541 acctcgtata gacagtggct caagcaaatc aagcaacagg gtgataccgt catcttttgg
    15601 ccatggtgca ccccatgtcc ggatatcctc aatccaagtt gttttcatca aagcggcacc
    15661 aatttctgga ataacggcaa taggtttttt atcagcagga atgaaaagaa accaggggcg
    15721 cgttgggctt tgccaaaact gcgtatggaa tccgctgaaa taacgaactt caggttctgt
    15781 cattagcaaa atcccagcta aatcttctgc tgccatcagt ttttgtactc gttttagacg
    15841 agcctcgaac tcactaacag gaaaacctcg aacagggcta tccatcattt ttgcctcctt
    15901 ttatgatctg gt
//
LOCUS       DTVD01000265           15895 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_719_length_15895_cov_403.420707, whole genome
            shotgun sequence.
ACCESSION   DTVD01000265 DTVD01000000
VERSION     DTVD01000265.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 15895)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15895
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_15895_cov_403.420707"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(147..1361)
                     /locus_tag="EYO68_09890"
     CDS             complement(147..1361)
                     /locus_tag="EYO68_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIC08149.1"
                     /translation="MPALLSSLKILDFSTLLPGPFASMILADLGAEVLRVESPTRPDL
                     VRMLPIQVKGGSATHATINRSKNSICLNLKNPDAVKIVKQLVSKYDIVLEQFRPGVMK
                     RLGLDYEELRRHNPALIFCSLTGYGQTGPYRNRAGHDLNYLALSGISNYSRRKNESPV
                     PLGIQIADIAGGSYHVVMGILAAVIHRQQTGEGQSVDISMADALFSMKVFESANWLAG
                     GPGAEPESTWLNGGTIYDYFLTQDGRWVSVSSLEPQFLAALINAMGLPETLFKTKLTD
                     PEAQRRLKRILRDRFLERPWTEWEPVFANCDACVELVLEFPEAMEHQQFKAREMITDV
                     PAPDGSSQKQIASPFKFSGSKPEYLHIGVELGEQTDIILKELGYNLKQIEKLRKDGVC
                     AGTEINNWGKDA"
     gene            complement(1363..1806)
                     /locus_tag="EYO68_09895"
     CDS             complement(1363..1806)
                     /locus_tag="EYO68_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HIC08150.1"
                     /translation="MFFIINEKNMKIPSFDSLKVGEEIPALITPPVTRHTLALYCGAS
                     GDHNPIHVDIDFAKSSGLDDVIAHGMLSTGYLARMITNWVPQSTLRSWKNRFTAMTHI
                     GDIVTCTGKITEKFEEGGEKLVRLELQTNTPQAQTIIAEAVVALE"
     gene            complement(1787..2230)
                     /locus_tag="EYO68_09900"
     CDS             complement(1787..2230)
                     /locus_tag="EYO68_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIC08151.1"
                     /translation="MIDKKHIGYEFLPVTVPVEEGRLKFLAKAIGETGDIYTNPDAAI
                     AAGLPGLLAPPTFPFMLEIDALDLVAFMSLLGESLEKLLHGEENFKYFAPIYAGDKIT
                     VCKKITDIIDKKEGALQFVISENTFTNQSGEIVAETRTNYVFHHK"
     gene            complement(2367..3095)
                     /locus_tag="EYO68_09905"
     CDS             complement(2367..3095)
                     /locus_tag="EYO68_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08152.1"
                     /translation="MFTRTDLAEVTSQEVNRENWRQQPEVLQLIQRYAHDLAGISNLT
                     DEMLSPEIAEIKLVGAVLGHEQTPNLNQESADGRVQFFQLSFQDGKTKKVSSKNWKKW
                     LIWLMIAVVVFLGMTSMFLIASRFSLLSLNSSKKSVSVCSTNRQEAKYSEHLRDIRKS
                     LVFELNRLPAWSTFNVARTYCIGGKMSVKQQEQRLLQCLLKVRNRTKNIPPASRPSLN
                     KIEACASTLCQRELPHLSEFCKRL"
     gene            complement(3259..3333)
                     /locus_tag="EYO68_09910"
     tRNA            complement(3259..3333)
                     /locus_tag="EYO68_09910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(3299..3301),aa:Gln,seq:ttg)
     gene            complement(3405..4082)
                     /locus_tag="EYO68_09915"
     CDS             complement(3405..4082)
                     /locus_tag="EYO68_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIC08153.1"
                     /translation="MKLFFRQTLILVWKDVLIDLKRKDNLLSMLLFAILTLLVFQFAM
                     GEEPDRFKMALPGIIWIVFLMSGVLGLEKSFMQEMETGCMGGLLLAPVERSVLFLGKM
                     LANTLFLLISQLLFIPLCLFLFEIEVINWLEFLMVILFGTIGFSSLGTLLTAMTSTLR
                     GKGMLLPILIFPLMVPSLLCVVRLTDFLFFGSHPAEVWSWWKLLIGFDVILFTFSLLG
                     FEFIVEE"
     gene            complement(4122..4478)
                     /locus_tag="EYO68_09920"
     CDS             complement(4122..4478)
                     /locus_tag="EYO68_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIC08154.1"
                     /translation="MPIYEYLCTSCGYQFEEVQKFSEPSLEECPDCGKNSAQRQVSMS
                     SFHLKGGGWYKDGYSSNVAEKENTERTGKEGSSTSSKESTTGEKPPAAEKLKTTEKKE
                     SNSNKIAKTSSKEKAA"
     gene            4577..5305
                     /gene="lptB"
                     /locus_tag="EYO68_09925"
     CDS             4577..5305
                     /gene="lptB"
                     /locus_tag="EYO68_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIC08155.1"
                     /translation="MSIRKLEATNLKKSYQGRHAVKGISLEVTQGQVVGLLGPNGAGK
                     TTAFYMIVGVQRPDEGTIFLDGHDITGYPMHKRAKAGLGYLSQERSIFRKLTVEENLI
                     AVLEMLPLSSSEKKDRLDSLLNELGIQHVRKIKGYALSGGESRRVEIARALVLEPAIM
                     LLDEPFAGVDPIAVADIQTIIRQLSKRNIGVLITDHNVRETFGIIDYGYIMNEGELLI
                     GGTPDELVENDQAKKVYLGKSFSM"
     gene            5425..7119
                     /gene="rpoN"
                     /locus_tag="EYO68_09930"
     CDS             5425..7119
                     /gene="rpoN"
                     /locus_tag="EYO68_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIC08156.1"
                     /translation="MAMQMKMQMKMSQQLIMTPQLQQAIKLLQLSRAELEELIEQTLI
                     ENPVLEEGIDMDTFPDKTEDSQETKETSDNPEVVTDTDEFETARENELNNKDQDEIDW
                     QQYIEQIEQFGGYQERRYNAVSGDEEGPSIEATAAQTESLADHLVWQLDMLELSPLEY
                     KTGAYLIGNIDDDGFLVTSIRELLESQYDLYELIIAQHKSGELPELPEVDPDLLDLKF
                     KSGKSTQKKKKKKEELSSFDEEEIIPEEEPKKLEVPPAVGAFVEYVLGKIQQFNPNGV
                     GARSLQECLLIQLDILGENDSLSTRIVKNDMPLLESKDLKKISRRQKQDIEQVIEAYR
                     LIMTLEPKPGREFISNPSHHYIIPDVYIYQKDDEYKVALNSAGMPRLRISNYYKDLSN
                     TMEDDGSLTKDYILEKVKAGQWLLKSIEQRQKTIYRVTKSILRFQKKFFEKGIHFLRP
                     LVLKDVAEDIDVHESTVSRITTNKYVHTPQGIFELKYFFTSGIDQGKGEAVSSKRIKD
                     MIMQMVQKENLKSPYTDLQIADILERKSGIKVARRTVAKYREALNILPSNKRKQLF"
     gene            7141..7851
                     /locus_tag="EYO68_09935"
     CDS             7141..7851
                     /locus_tag="EYO68_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIC08157.1"
                     /translation="MGIISRLFNLFRANANDMLDKAEDPEKMLQQMLSDLEVQKQKAK
                     KQMTEALALQKRLERDTEKEHKEADKWEQKAILAVQNEKDDLAKEALTRKKEFTIRAA
                     DYEKQLEMHRNNADALRSSYQTLEDKIDEIKRKKGILSVKQKQAEAQENIYQTMEGLG
                     DTSGTMETIARIEEKVENMQTRAEAYQEISMESDKDSLESKFEELEHDSSDMELELLE
                     LKKRAALPAPDEESDKKE"
     gene            7937..8155
                     /gene="rpsU"
                     /locus_tag="EYO68_09940"
     CDS             7937..8155
                     /gene="rpsU"
                     /locus_tag="EYO68_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIC08158.1"
                     /translation="MPIKVEVRDGNVGRSMMQLKRTLIREGLFKEIKKRKFHCKPSLA
                     KRLKREAAAKQRNKDLKREIRAALKADF"
     gene            complement(8346..8507)
                     /locus_tag="EYO68_09945"
     CDS             complement(8346..8507)
                     /locus_tag="EYO68_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HIC08159.1"
                     /translation="MAAELKASFGVDSELLSGEKGDFEVVVDGKTVFSKKKLSRFPEP
                     GEVTETLRE"
     gene            complement(8761..9312)
                     /locus_tag="EYO68_09950"
     CDS             complement(8761..9312)
                     /locus_tag="EYO68_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08160.1"
                     /translation="MLSADFQDPTVFNQLYPSEGVQNWAGLIGALIGGSLLEIFGPSA
                     LLLPWLFVRAALHQSRQISGLICTYYAFVLVFLLSIIHELALHSGLIKTEALEFYLQN
                     GYAAKLALIWLEKSTNLAISLTVLAAMFIFSLVRMSQIISPLPFISGVFSGVQILLSM
                     LARKISPPPPADNYSTTQLPNFK"
     gene            complement(9409..9897)
                     /gene="coaD"
                     /locus_tag="EYO68_09955"
     CDS             complement(9409..9897)
                     /gene="coaD"
                     /locus_tag="EYO68_09955"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIC08161.1"
                     /translation="MRIAVYPTSANPPTYGHADILVRTTKQFDHVFWSAAMNSEKHYM
                     FNEEVRLRMMNEYVQHFDLKNVTVEAFTGSTIRYVQKRKAQVIVKGLRSLDDFQGEFQ
                     QAVGNKGIDPEIETFCLFGKPDLFAINSTLVRELAFLGESIEAYVLPSVAKIVADAIK
                     KS"
     gene            complement(9942..12722)
                     /gene="mutS"
                     /locus_tag="EYO68_09960"
     CDS             complement(9942..12722)
                     /gene="mutS"
                     /locus_tag="EYO68_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIC08162.1"
                     /translation="MSVPQAETPMMRQFNAIKREHPDKLLFYRMGDFYEMFGDDAIVG
                     AKVLQIALTSRDKKKKKSLPMCGVPYKAYEQYLNKLTAAGYKVAICEQMEDPAKAQGL
                     VARDVVRVVTPGTTVSPQLIDPNRNHFLLAINVVLRAQCLGIAFVDLSTGEFEVAEFH
                     LNETHRFYDFLAQLTPQEILLTQSRSEIESTFLEELVQRMTQLLDKINSADARSVTSS
                     EQISGLQNAQVFPSTGRFNFIDPYHFDPEATQRNLNNHFETLNLSGFGVDDLPHGIAA
                     AGALLRYLEETQKCDLSHLTALRRYAFDKTMLLDEATVANLELFESQSGARKHTLYHI
                     LNHTKTPMGARIFRQWLRQPLLDVEAINERFDCIEEFRNNFMLCEEFRECLENIQDLP
                     RIIGRITLPVVGVNDLVALRESLEPVQKLPTYLAELQAPLLKKIAENFDPLTDLLDLL
                     YQRLLEAPSVKLREGGFMAAGVSEELDELREISRNSKQFLNEMLLHEREITGISSLKI
                     SYNKVFGYYLEVSNVHKSSVPEHYIRKQTLVNAERYITAELKEFEEKILTAEERIGEL
                     EYELFQQLKIEINSNARRVQQTANDIAVADTLAGLAFVAENNHYVRPSLHPLEAPRKL
                     FLKDSRHPVIEQIDLGEVFVPNDLDMEESKCRIMLITGPNMAGKSTYMRQVALIVLMA
                     QCGSFVPVSSSEFSVVDRIFTRVGASDNLTLGQSTFMLEMNEAAAILNNATERSLIIL
                     DEIGRGTSTFDGISIAWAIVEYIQKLSALTLCATHYHELTAIAQELDSVKNFSIRVEE
                     EGEKIVFLRKIVSGEADKSYGVQVARLAGLPKSVVNRALEVMEQLEETSMVHHLPVLI
                     DETQQKQKNSAEVSENSGSSWVNSVNEDQQLSLFPEESLYLDELRKLNLNDMTPLQAL
                     NYLHELTDKLRQ"
     gene            complement(12754..13566)
                     /locus_tag="EYO68_09965"
     CDS             complement(12754..13566)
                     /locus_tag="EYO68_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HIC08163.1"
                     /translation="MPVSISETDIENYAKDGALLIKNLFTPDEVSDLREGIDANISHP
                     SSRAKVASNESDPGWFFEDFCNWQENSAFQRIIFESDISEVAAKLMCSEQVRLFHDHM
                     LVKKTGTKQRTPWHQDQPYYNIEGMQNISFWIPVDPVPLEWTLEFIAGSHQENWYLPR
                     TFLKKEAKWFPEGSLLDPPNIEDNPEKYRVLSWELEPGDAVAFHMLTLHAGAGSGALR
                     RVFSVRLIGDDIRHAPRDWETSPEFPGLSDQLPAGVPMDHELFPVIWPASRA"
     gene            complement(13574..14350)
                     /locus_tag="EYO68_09970"
     CDS             complement(13574..14350)
                     /locus_tag="EYO68_09970"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIC08164.1"
                     /translation="MNLTEYLNNRLKKHKILLMTHAVVGYPSLEENWKMLEAMGEADV
                     DLVELQMPFSEPSADGPLFVKANQDALRNGVRWKDYFDLMKSASERFNFPVLMMNYCN
                     TAFSMGFEAYCSNIRIHGGEGFIIPDLPFEEFGELFELSRDQELDPIMLCTPTNTEKR
                     LQNICEKGSGFIYCVARKGVTGQNTELDRHADDFLRQCRKYSNLPLALGFGLSKPEDL
                     CQLHGKAEIAIIGSALLKTWEAEGEPGYRKHLMSLADARD"
     gene            complement(14412..15731)
                     /gene="trpB"
                     /locus_tag="EYO68_09975"
     CDS             complement(14412..15731)
                     /gene="trpB"
                     /locus_tag="EYO68_09975"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIC08165.1"
                     /translation="MTSKENVRKIYLWIIKQKIERYNIISMPTQTISPDISGYYGDYG
                     GMFIPEILRTTFEELISAFKEAKADPEFWQSYVNVMQNYSCRPTPVTPLENFAKSLGD
                     RFQVFCKREDLNHTGAHKANNVMGQGLLVQRMGKQRVIAETGAGQHGVATATMAAKMG
                     FECTIYMGSVDVERQRPNVFWMEQLGAEVISVTEGTATLKDAINAAMRDWAESMETTH
                     YVLGTTCGPHPFPEMVSWFQSIIGHESREQMLKLNSKLPDRIYACVGGGSNASGIFLP
                     FLEDQSVELVGVEAGGHGIDSGKHAARFSGGTVGIAQGYKTYFFQDDEGQMLHTHSIA
                     AGLDYIGVSPILSHLHDKKRIRFTSATDEEVISATKLLIRTEGIIPALESGHAFASVI
                     EEARDIPDGAQVLINLSGRGDKDIFNIAEAIGDHKWQEFLEKKALKS"
     gene            15742..>15895
                     /locus_tag="EYO68_09980"
     CDS             15742..>15895
                     /locus_tag="EYO68_09980"
                     /inference="COORDINATES: protein motif:HMM:PF11013.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HIC08166.1"
                     /translation="MIKNNSFPEMAIQQIWLEQDFDQVTLKTICGQQVKIEFAGWYNS
                     ASGPDFR"
BASE COUNT         4825 a         3422 c         3049 g         4599 t
ORIGIN      
        1 tatttcatta atatatgaag cagataaccc agtcagtacg gcaagttgtt taagaccaaa
       61 tcctttttat tgtctaaaac agcggagctt catcccaaaa aaaacgtaaa ttaaatttac
      121 ctgagttaga tgtcagctct ggtttattac gcatcttttc cccaattatt tatttcagta
      181 cctgcacaaa ctccatcttt tcttaatttt tcgatttgtt tcaaattata accaagttct
      241 ttcaaaataa tatccgtttg ctcaccaagc tctacaccaa tgtgcagata ttctggtttg
      301 cttccggaaa acttaaatgg ggatgcaatt tgtttttgag aagaaccatc cggagccgga
      361 acatctgtaa tcatttctct ggctttaaac tgctgatgtt ccattgcttc cggaaattcc
      421 aaaaccaatt caacgcacgc atcgcaattg gcaaaaacag gttcccattc agtccacgga
      481 cgttccaaaa atcgatcccg gagtatcctc tttagacgcc tttgtgcttc cggatcagtc
      541 aattttgttt tgaacaaagt ttccggaagc cccatagcat ttatcaatgc agcaagaaat
      601 tgaggttcca aactactaac agaaacccag cgaccatctt gcgttaggaa ataatcataa
      661 atagttccgc cattgagcca ggttgattct ggttctgctc ccggtcctcc tgccagccag
      721 tttgcacttt caaatacttt catgctgaac aaagcatctg ccatacttat atcgacactc
      781 tgtccttctc ctgtttgctg gcgatgaatt actgctgcta aaattcccat tactacatga
      841 tatgaacctc cagcaatgtc cgcaatctga atacccagcg gcacaggtga ttcatttttt
      901 cgacgactgt aattacttat tccggaaaga gcgagataat tcaggtcatg accggcacgg
      961 ttacggtaag gtccggtttg cccgtaacca gtcagtgaac aaaaaatgag tgcaggattg
     1021 tgtctgcgga gttcctcata gtccaatccc aaccgcttca tcaccccagg acgaaactgt
     1081 tccaaaacga tatcatattt gctgaccaat tgtttaacaa ttttgacggc atcaggattt
     1141 ttcaaattta agcaaatgga gtttttggaa cgattaattg tcgcatgagt tgctgaacca
     1201 ccttttactt gaatcggcag cattctcact aaatccggtc gtgtgggaga ttctaccctt
     1261 aaaacctctg cccctaaatc tgcaagtatc atcgaggcaa aaggtcctgg cagtaacgtc
     1321 gaaaaatcaa gaatttttaa tgaggaaagg agagcaggca tattattcca gggcgaccac
     1381 agcttctgcg ataatggtct gtgcctgtgg ggtgtttgtc tgtaactcaa ggcgcaccag
     1441 tttttctccg ccttcctcaa acttttcagt aattttgccg gtgcaggtta ctatgtcacc
     1501 gatatgagtc attgcagtga aacgattttt ccagctcctc agagtagatt gtggaaccca
     1561 gttggtgatc atcctggcca ggtatcctgt tgaaagcatt ccgtgtgcaa ttacgtcatc
     1621 cagtccagat gatttagcaa aatcaatatc cacgtgaatc ggattatggt ctccggaagc
     1681 accgcaataa agtgccagcg tatgccgggt tactggagga gtgattagag caggaatttc
     1741 ttctcctact tttagagagt cgaatgaagg tattttcata tttttttcat ttatgatgaa
     1801 aaacataatt tgtgcgggtt tcagctacaa tttcacctga ctgattggtg aaagtgtttt
     1861 ctgaaatcac aaactgtaaa gctccctctt ttttatcaat gatatcagta atttttttgc
     1921 agacagtgat tttatcgcct gcatatattg gtgcaaagta tttgaaattt tcttctccgt
     1981 gcagcaattt ttccaggctt tctcccaaca aactcataaa tgccaccaaa tccagtgcgt
     2041 ctatttccag cataaaagga aaagttggag gtgccagcaa tcccggaagt ccagcagcta
     2101 ttgctgcatc cggatttgta taaatatccc cggtttcccc aattgctttt gccaaaaatt
     2161 ttaaccgccc ctcttcaact ggtacagtta ctggaaggaa ttcatagcct atgtgttttt
     2221 tgtcaatcat gttttcagtg taaatttgcc gtgttattgc tcataaaata ttttttgcat
     2281 ttattaattg gtttttttac aaatcattga tgatacacgt gatatttaac tttttatttc
     2341 tcaattacaa atgttagtag agtatatcac aatcttttgc agaactcaga caggtgcgga
     2401 agttcccttt gacagagagt acttgcacaa gcttcaattt tgttaagact cggtcttgaa
     2461 gccggcggta tgttttttgt gcggttacgt accttcaaca aacactgaag caaccgctgt
     2521 tcctgctgtt tgacactcat ttttcctcca atacagtaag tacgcgccac gttaaaagtt
     2581 gaccatgctg gtagacggtt cagctcaaaa accaatgatt tccgtatgtc tcttaaatgt
     2641 tctgaatatt ttgcttcctg tctgtttgtg ctgcaaactg aaacagattt tttgctgctg
     2701 ttcaaagata acaatgaaaa ccgactggca atcaagaaca tcgaggtcat tcctaaaaaa
     2761 actactactg caatcatcaa ccaaatcagc cactttttcc aattttttga cgaaactttt
     2821 ttggtttttc cgtcttgaaa actaagctga aaaaactgca ctcttccatc tgcggattct
     2881 tggttcaaat ttggcgtttg ttcatgaccg agaactgctc ctaccaattt tatttctgca
     2941 atttccggag aaagcatttc atctgtcaaa tttgaaattc ctgccagatc atgtgcatag
     3001 cgttgaatta gctgcagaac ttctggttgt tgacgccaat tttcccgatt tacttcctga
     3061 gatgtaactt ctgcaaggtc tgtcctggtg aacatcttgg ctttccgctc tttccctaca
     3121 cctgtgtcac atacgccaat aggacgccat tgaatgtatt ttagtcggga tatatcagtc
     3181 atgttgaggg aaatgaatat tcccaatgca gggaggactg aataggaaaa gagttgatgt
     3241 gcttatcaat ataatgactg gctggggcgg cagggatcga acctgcgaat cacgggatca
     3301 aaacccgatg ccttaccact tggctacgcc ccaaataacc aataattaag gcttcttatt
     3361 ctatcttggc aatgaaaatt tgcaagagat tttctgagta aaagttattc ttcaacaata
     3421 aattcaaatc ccagtaaaga aaaagtgaag agaataacat caaaacctat aagcagtttc
     3481 caccaggacc agacttccgc aggatgagag ccaaaaaata gaaaatccgt aagacggaca
     3541 acacataata gacttggaac catcagggga aaaataagaa tcggtaaaag cattcctttc
     3601 ccgcgtaaag ttgatgtcat tgcagtcaac aaagttccta atgaactgaa tccaattgtc
     3661 ccaaaaagaa taaccattaa aaactctaac cagtttatta cctcaatctc gaaaagaaac
     3721 aggcatagag ggataaacaa aagctgcgaa atcaaaagga acaaggtatt tgccagcatt
     3781 ttacctaaaa ataaaacact acgttctact ggtgccaaca acaaccctcc catgcagcca
     3841 gtttccattt cctgcataaa agatttttcc agtccaagca caccgctcat caggaacaca
     3901 atccagataa tacctggaag tgccatctta aatctgtctg gttcctctcc catcgcaaat
     3961 tgaaagacca gcaaagtcag aattgcaaac aagagcattg aaagcaagtt gtctttacgt
     4021 ttcaaatcaa tcagtacatc tttccagacc aaaattaaag tttgtctgaa aaacaatttc
     4081 atgatgagga ttagacttta aggaagaagt ggagaaaata tttaggcggc tttttctttg
     4141 ctagaagttt tagcaatttt atttgaattt gattccttct tttctgttgt ttttaatttt
     4201 tcagcagctg gcggcttttc cccggtagtc gactccttac ttgatgtgga actgccttcc
     4261 tttccggttc tttctgtgtt ttctttttca gccacgttgc tggaatatcc atctttgtac
     4321 cagccaccac ctttaaggtg aaatgaactc atcgaaacct ggcgttgtgc actgtttttc
     4381 ccgcaatccg gacattcttc caaggaaggt tcactgaatt tttgcacttc ttcaaactgg
     4441 tatccacaac tcgtacagag gtattcgtag attggcatat aattctcttt ctgctacaat
     4501 taatttttac tacagctagg aagactatac aaacaaacaa acaaattgtc aattcagagt
     4561 tacccggaga cttggtttga gtataagaaa attagaagct actaacctga aaaagtcata
     4621 tcagggcaga catgcggtca aaggtatctc tcttgaagtc actcagggac aggtcgttgg
     4681 ccttcttggt cctaatggtg ctggtaagac gacagctttt tacatgatag ttggtgtaca
     4741 gcgtccggac gaaggaacca tctttttgga cggtcatgat atcacaggat accccatgca
     4801 taagagagct aaagccggcc tcggttatct ttctcaagaa cgttcaattt ttcgaaaact
     4861 cactgtagag gagaacttga ttgcagtgtt ggaaatgctt ccgctgagta gttcagaaaa
     4921 aaaagatcgt ctagactctc tgctaaacga acttggaata cagcacgttc gaaaaataaa
     4981 aggatatgcg ctttctggtg gagaaagcag gcgcgtagaa atagcacgtg cacttgtgct
     5041 tgaaccggcg atcatgttgc tggatgagcc cttcgcaggt gtggatccaa ttgcagttgc
     5101 tgatattcaa acaatcatta gacaactttc aaagcgcaat atcggagtat taatcacaga
     5161 ccacaatgtc cgagagactt tcggaattat cgattacggc tacattatga atgaaggaga
     5221 gttgttaata ggcggaactc cggatgaatt agtcgaaaat gatcaggcca aaaaagttta
     5281 tctcggcaag tctttctcta tgtaggtttg actttttcct caacttggag tgaacttaat
     5341 actaagcata ttaagatcat ttcttaatca gtcaattttg aaaataattt tcataactct
     5401 aagaaagtag gatagcgagg attcatggca atgcagatga aaatgcagat gaagatgagc
     5461 cagcagctta tcatgacacc tcaactgcaa caagccatca aactccttca attgtctcgt
     5521 gctgaattgg aagagttaat tgaacaaact ctaatcgaga accctgttct tgaagaaggt
     5581 atagatatgg ataccttccc tgacaaaaca gaagattcac aagaaaccaa ggagacttca
     5641 gacaatcctg aagtagttac cgatacagac gaattcgaaa ctgcccgtga aaacgaactg
     5701 aataataaag atcaggacga aattgattgg cagcagtata ttgaacaaat agagcagttt
     5761 ggaggttatc aggaacgtcg atataatgct gtttcaggcg acgaggaagg cccctcaatt
     5821 gaagccacag ccgcacaaac tgaatcttta gcagatcacc tggtatggca gttagacatg
     5881 cttgaacttt ctccccttga atataaaaca ggtgcatact tgattggtaa catagatgat
     5941 gatggttttc tggtaaccag tatccgagaa ctgttagaat cacagtatga tttatacgaa
     6001 ctgattattg cacaacataa aagtggtgaa ttacctgaac taccagaagt tgatcctgat
     6061 ttgttagacc tgaaatttaa atctgggaaa tcaacacaaa agaaaaaaaa gaaaaaagaa
     6121 gaactttcta gctttgatga agaagagatt attccggaag aagaaccaaa aaagcttgaa
     6181 gttccaccgg cagtcggtgc cttcgtagaa tatgtattgg gtaaaataca acaattcaat
     6241 ccgaacgggg ttggcgccag aagtttacag gagtgtctgt taattcagtt ggatattttg
     6301 ggcgaaaatg acagtttgag tacccgcata gtcaaaaatg acatgccgct actggaatca
     6361 aaagatctta aaaaaatttc ccgcagacaa aaacaggata tcgaacaagt gattgaggct
     6421 taccgtttga taatgactct cgagccaaaa cctggaagag agtttatttc caatccatca
     6481 catcattaca taattcctga tgtctatatc tatcaaaaag atgacgaata taaagttgcg
     6541 ctcaactccg cgggtatgcc acgtctccgt atcagtaatt attataaaga tttgagcaac
     6601 actatggagg atgacggtag cctcacaaaa gattatattt tagaaaaagt taaagcgggt
     6661 caatggctgc taaaaagtat agagcagaga caaaaaacta tttaccgtgt gactaaaagc
     6721 atactccgct ttcaaaagaa attctttgaa aaaggtatcc actttttgcg accgcttgtg
     6781 ctgaaagatg ttgctgaaga tattgacgta cacgagtcga cggtcagccg gatcaccacc
     6841 aataaatatg ttcacactcc tcaaggaatc tttgaattaa aatatttctt cacaagtggt
     6901 attgatcaag gtaaaggaga agcagtttct tcaaaacgga taaaagatat gatcatgcaa
     6961 atggtgcaaa aagaaaatct taaatcacct tatacagatt tgcagatcgc agatattctg
     7021 gagcgtaaaa gtggaattaa agttgctcgt agaactgtag caaaatatag agaagccctc
     7081 aacatccttc cttcaaacaa acgtaaacaa cttttctaga accttcattc agaaaaaatt
     7141 atgggaatca tctctcgact atttaatcta tttcgagcaa atgctaatga catgctggat
     7201 aaagcggaag atccagaaaa aatgcttcag caaatgcttt ctgatttgga agtgcaaaaa
     7261 cagaaagcta aaaagcaaat gactgaagct ttagctttac agaaacgttt ggaacgcgac
     7321 acagaaaaag aacataaaga agcggataaa tgggagcaaa aggctatcct tgctgtccaa
     7381 aatgaaaaag atgatttggc taaagaggcc ctgacacgga aaaaagaatt tactatccga
     7441 gccgctgatt atgaaaaaca acttgaaatg caccggaaca atgcggatgc cctccggagc
     7501 agctaccaaa ctttggaaga taaaatagat gagattaaac gcaagaaggg aatcttatct
     7561 gtaaaacaaa aacaggcaga agctcaggaa aatatttatc aaacgatgga aggtctagga
     7621 gacacttccg gtacaatgga aacgattgca cgaattgaag aaaaggtgga aaacatgcag
     7681 actagagctg aagcttatca ggaaatcagc atggaatctg acaaagacag tttggaaagt
     7741 aaatttgagg aattggaaca tgattcctct gatatggaat tggaattact tgaattgaaa
     7801 aaacgggctg cgcttcctgc tcctgatgaa gaatctgata aaaaagaata aaaatattgc
     7861 atttgttaaa ttaatgcgtg ataattagga gttaaaatca taacatttca tttctaaatg
     7921 cattaaaagg tagttcatgc caataaaagt tgaagttcgc gacggcaacg tcggcagatc
     7981 aatgatgcaa ctgaagcgca ccttgatcag agaaggactt tttaaggaaa tcaagaaacg
     8041 aaaatttcac tgcaaaccgt ctttggcaaa acgtctcaag cgtgaagctg cagcaaaaca
     8101 gcgtaacaaa gatctgaaac gtgaaatccg tgcggctctt aaggctgatt tctaagatta
     8161 ttctgtacta aaataaggcc ccattttcag gtaaaccctc caaccttttt ttcctgacga
     8221 ttcttagctg atattggcaa attgtgtctt acagattagc aggtacaaac tgctggtttt
     8281 ttttgaggat ataagagaca ttgcagttta atagtcgttt gatgacatag ctgttattta
     8341 agtattcatt ccctgagtgt ctctgttact tctccgggct cagggaacct tgacagtttt
     8401 ttcttggaga aaactgtttt accatccaca actacttcaa agtcaccctt ctcacccgaa
     8461 agaagttcgg aatccacacc aaaggatgct ttcaattccg cagccaaact gactgcgctg
     8521 ggcttgtagt ttcagaccac acagtagtat atttttatat tcatatttct gtttatcatt
     8581 aaaaagtttt taatttatta ttgccacaaa gaactttgac tgcatcagtt ttacggttta
     8641 agattgactg gattctaagt aaaaatcaag acctggacga tcaaaaataa attaaattac
     8701 aattattttt aatcagataa taaatcacaa atccaaaagc aacgccattt aaacgtaaag
     8761 ttacttaaag ttgggcagtt gtgttgttga ataattatct gcaggtggag gggggctaat
     8821 ttttctggca agcattgaga gaaggatttg tactccagaa aatactccag aaataaaagg
     8881 gagcggcgaa attatttgag acattctgac taaagaaaaa ataaacatcg cagccaaaac
     8941 agttaaactt atggcaagat tagtcgattt ctctaaccaa attagagcta attttgctgc
     9001 atacccattt tgtaagtaga actctaaggc ttctgttttt ataagaccgg aatgtagtgc
     9061 aagttcatgt attattgaaa gtaaaaacac aagtacaaat gcataataag tacatatcaa
     9121 tccggaaatc tgacgtgatt gatgcaaagc agcccggaca aaaagccacg gaaggagcaa
     9181 agccgaaggt ccaaaaattt ccagcaaaga accaccgatt agtgctccga tcaaacctgc
     9241 ccagttctgc actccttctg aaggatacaa ctgattaaaa acagtcggat cctggaaatc
     9301 tgcagacagc atagacaaga atagggctac gcctattcct ccgaaaaaca aatagtagat
     9361 tcgtagtttg atgtcactca tggtttttta ctctctgatg tttccgaatc atgatttttt
     9421 tattgcatca gccacaattt ttgccacaga aggtaaaaca taagcttcga ttgactctcc
     9481 cagaaatgct aattcacgaa ccagggtcga gttgatggca aaaagatccg gttttccaaa
     9541 taagcaaaag gtttcaattt caggatcaat gcctttgttt ccgactgctt gctgaaattc
     9601 accctgaaaa tcgtctaaac tacgcaatcc tttcactatt acctgagctt ttcgtttttg
     9661 cacgtagcga atagtagaac ctgtgaaagc ttcaactgtt acatttttca ggtcaaaatg
     9721 ctgcacgtat tcattcatca tccgcagacg tacttcttcg ttaaacatat agtgtttttc
     9781 cgaattcata gcagccgacc aaaaaacatg atcaaactgt ttggtcgttc tcaccaatat
     9841 atctgcatga ccataagttg gaggatttgc acttgtcggg taaacagcaa ttcgcatttt
     9901 aggattcaag tttaaaattt tgatactacc ggcttttatt gctattgccg tagtttgtca
     9961 gttaattcat gaagatagtt tagtgcctgc aacggggtca tatcattcaa attcagtttg
    10021 cgcaactcat caaggtagag tgattcctct ggaaacaagg acaactgttg atcctcattt
    10081 acagaattta cccaagaact tccagaattt tcactaacct ctgcagaatt tttttgtttc
    10141 tgctgtgtct catctataag tacaggcaaa tggtggacca ttgacgtttc ttccaattgt
    10201 tccataacct caagagcacg atttaccacg ctttttggca gtccagccag cctggctact
    10261 tgtactccgt aacttttgtc ggcttctcct gaaacaattt tacgcaaaaa aacaattttc
    10321 tctccttctt cctcaacacg aatgctgaaa ttttttactg aatccagttc ttgtgcaatt
    10381 gcagtcaatt catggtaatg agtagcacaa agcgtcaggg cactcaattt ttggatatac
    10441 tctacaattg cccaagcaat actaataccg tcgaaagtac tcgttccacg tccgatttca
    10501 tccagaataa tcaaacttct ttcagtcgca ttgttcaaaa ttgcagcagc ttcgttcatt
    10561 tccagcataa aagtactttg accaagtgta agattatctg atgcaccaac acgtgtaaaa
    10621 attctatcca ccacagaaaa ttctgagctc gaaacaggca caaaacttcc acattgagcc
    10681 atcaggacaa ttagcgcaac ttgtctcatg taggttgatt tgcctgccat attcggccct
    10741 gtgattaaca tgatccgaca ctttgattct tccatatcca aatcattagg cacaaacact
    10801 tcgcccagat ctatttgttc aataacagga tgtcgactgt ctttcaaaaa caattttcta
    10861 ggagcttcaa gaggatgtag actgggtcgg acatagtgat tgttttctgc gacaaatgcc
    10921 agtcccgcca gcgtatccgc tacagcaatg tcatttgcag tctgctgcac tcgtctggcg
    10981 tttgagttga tttcaatttt tagctgctga aatagttcat actccaattc acctattcgt
    11041 tcttctgcag taagtatttt ttcttcaaat tctttgagtt cagcagttat gtaacgttca
    11101 gcattgacca gggtttgttt acgaatgtaa tgttccggga cagaagattt atggacatta
    11161 ctgacttcaa ggtagtaacc aaaaactttg ttgtaactga tttttaaaga gctaattccg
    11221 gtaatctccc tttcatgcag cagcatttcg ttaagaaatt gtttggaatt tcttgatatt
    11281 tcacgtaact cgtccaattc ttcagaaaca cctgcggcca taaaaccacc ttctcgaagt
    11341 ttaactgaag gtgcctccag tagtcgctga tacaacaggt ctaacaaatc ggtgagcgga
    11401 tcaaaatttt ctgcaatttt ttttaacaac ggcgcttgta actctgccaa ataggtaggc
    11461 agtttctgca ctggttccag tgattcacgt aaagccacaa gatcgttcac accaaccacg
    11521 ggtaaagtaa tgcgtccaat aatgcgcggc aaatcctgga tattttccag gcattcacgg
    11581 aactcttcac aaagcataaa attattccgg aactcttcaa tacagtcaaa acgctcatta
    11641 attgcttcca catccagcag cggctgacgt aaccactgcc tgaaaatcct tgcacccatt
    11701 ggagtcttag tatgatttag aatatgataa agcgtgtgtt tcctcgctcc ggactgactc
    11761 tcaaacaatt ccagattggc cactgttgct tcgtcgagga gcatagtttt atcaaaagca
    11821 tagcgcctca aagctgtaag atggctcaaa tcacattttt gagtttcctc caaatagcgt
    11881 aataatgccc ctgcagcagc aattccatgc ggcaaatcat caaccccaaa tccggaaaga
    11941 ttcagcgttt caaaatgatt gttcaaattc cgttgtgttg cttccggatc aaaatgatat
    12001 ggatcaatga aattaaaacg tcctgtcgaa ggaaaaacct gagcattctg tagccctgag
    12061 atctgttccg aagaagtcac ggatctggca tcggcagaat ttatcttatc cagtaattgt
    12121 gtcatccgct gcaccaattc ttccaaaaac gtggattcta tttcagaacg gctttgcgtt
    12181 aacagaattt cctgtggagt aagttgtgca agaaaatcat agaaccgatg agtttcattt
    12241 aaatgaaatt ctgccacctc aaactcacct gttgaaagat caacaaaggc aattcctaaa
    12301 cactgggcac gcaaaacaac attgattgcc agcaaaaagt ggtttctgtt tggatctatc
    12361 aactgcggcg aaacagtagt tccgggagtt acaactctca caacatcccg tgcaacaagc
    12421 ccctgtgctt ttgcaggatc ttccatttgc tcacaaatgg ccaccttata acctgccgca
    12481 gtcaacttat ttaaatattg ctcataagcc ttatatggta ctccgcacat tggcaatgat
    12541 tttttctttt ttttgtcacg tgaggtaagc gcaatttgca aaacttttgc gcccacaatc
    12601 gcgtcatctc caaacatttc ataaaagtct cccatacggt aaaacaaaag cttatccggg
    12661 tgctcccgct tgatggcgtt aaactgacgc atcatcggag tttctgcttg aggtactgac
    12721 atgggtttga atttagaggt agaaaagtat catttaagcc ctacttgcag gccagattac
    12781 tggaaataat tcgtgatcca ttggaactcc ggcaggcaat tgatctgaaa gtcccggaaa
    12841 ctcaggagaa gtttcccagt cacggggagc atgtcgaata tcatctccta tcaagcgtac
    12901 tgaaaaaacg cggcgcagcg cacctgatcc cgctccggca tgcaatgtaa gcatgtgaaa
    12961 ggccacagcg tctcccggct caagctccca gctgagaaca cgatattttt ccggattatc
    13021 ttcaatattt ggaggatcca aaaggcttcc ttccggaaac cactttgcct cttttttgag
    13081 gaaagtcctt ggcaaatacc agttttcctg atgagatccg gctatgaatt caagtgtcca
    13141 ttcaagcggt actggatcaa ccggaatcca aaaactgata ttttgcattc cttcaatatt
    13201 gtagtacggt tgatcttgat gccatggggt cctttgtttc gttcctgttt ttttaaccag
    13261 catatggtcg tgaaacaaac gaacctgctc agaacacata agttttgcag caacttccga
    13321 aatatcactc tcaaaaatta ttctttggaa agccgaattt tcctgccaat tacaaaaatc
    13381 ctcaaagaac cagccagggt cactttcatt gctggcaacc tttgcacgtg aactgggatg
    13441 cgagatgttt gcatcaattc cttcccgcaa atctgacact tcatctggag taaacaggtt
    13501 tttaatcagc agtgctccat ctttcgcgta attttcaata tcggtttcac tgatggatac
    13561 aggcataatt tatttaatcc ctggcatccg ccagtgacat caaatgtttg cggtagcctg
    13621 gttcaccttc agcttcccac gttttcaaca aggcagagcc gataatagca atttcggcct
    13681 ttccgtgcag ttggcacaaa tcttcaggct ttgacaaacc aaatcccaga gctaatggca
    13741 aattagagta tttccgacac tgacgcaaaa aatcatctgc atgccggtcc aactccgtat
    13801 tttgtccagt tactccttta cgtgctacac aataaataaa accactgccc ttctcacaga
    13861 tattttgtaa tcgtttctcg gtatttgttg gagtacaaag catgatcgga tcaagttcct
    13921 gatcacggct cagttcaaac aattctccaa attcttcaaa aggtaaatcc ggaataatga
    13981 aaccttcacc accgtgtatc ctaatattgg aacagtatgc ctcaaacccc atactgaaag
    14041 ccgtgttaca ataattcatc atcaaaaccg gaaaattaaa tctttcgctt gcactcttca
    14101 tcaaatcgaa ataatctttc cagcgaacac cgtttcttaa tgcatcctga tttgctttta
    14161 caaaaagagg tccatccgca cttggctcac taaacggcat ctgcagttcc accaaatcca
    14221 catctgcctc acccatcgct tccagcattt tccaattctc ctccagcgaa ggatagccta
    14281 ctacggcgtg ggtcattagc aaaattttat gtttttttaa tcgattattt agatattcgg
    14341 tcagattcat ttaatttatg gattacgaat tacaacacca cagatgctct gcttttcggt
    14401 gccttagtgc ctcagctctt cagtgccttt ttttctaaaa attcctgcca tttatggtcg
    14461 ccaattgctt ctgcaatatt gaaaatgtct ttgtctccac gaccggaaag attgatcaga
    14521 acttgcgctc catccggaat gtctcgagct tcctcaatca ctgaggcaaa ggcgtgacca
    14581 ctctccaaag ctggaattat cccttcggta cgaatgagaa gttttgttgc agaaataacc
    14641 tcttcatcag tggcagaagt aaagcgaatg cgcttcttat cgtgcagatg actcagaata
    14701 ggactgactc caatgtagtc cagaccagcg gcaattgaat gcgtgtgcag catctgccct
    14761 tcatcatcct gaaagaaata cgttttgtaa ccttgtgcga ttccaacagt cccaccggaa
    14821 aaacgggctg catgctttcc ggaatcgata ccatgacctc cggcttctac cccaaccaat
    14881 tcgactgatt ggtcctcaag aaacggcaga aatattccgc tggcgttgga tccacctcca
    14941 acgcaggcat aaattctgtc tggcagttta gagtttaatt taagcatttg ctcacgtgat
    15001 tcatgaccaa taattgactg aaaccaggac accatttccg gaaatggatg cggcccgcaa
    15061 gttgttccca aaacataatg cgtagtttcc atgctttcgg cccagtcccg catggctgca
    15121 ttgatcgcgt cttttaatgt ggcagtacct tcagttactg aaataacttc tgctccaagc
    15181 tgttccatcc aaaatacatt cggacgctgg cgttccacgt caacagaacc catgtagatc
    15241 gtgcattcaa atcccatttt tgctgccatg gttgcggtag cgacgccgtg ctgtcccgcc
    15301 cccgtttctg caatcactcg ttgttttccc attcgttgaa ctagcaaacc ttgccccatc
    15361 acattattcg ctttatgcgc accggtatgg ttcaagtcct cccgtttgca aaaaacctga
    15421 aacctgtcgc ccaatgattt tgcaaaattt tccagcggag ttactggagt tggacgacat
    15481 gagtagtttt gcataacgtt cacgtaactt tgccaaaact cagggtcagc ctttgcttct
    15541 ttaaaagctg aaatgagttc ctcaaacgtg gtccgcaaaa tttcgggaat aaacattccc
    15601 ccgtaatcgc cataatatcc ggaaatatcc ggtgaaatgg tttgggttgg catagagatg
    15661 atattgtatc tttcaatttt ctgttttatg atccaaaggt aaattttacg tacattttct
    15721 ttacttgtca attagccttt catgattaaa aacaactcct ttcctgagat ggctatccag
    15781 cagatttggc tggaacagga ctttgatcag gtgactctga agacgatctg cgggcagcag
    15841 gtcaaaattg agttcgcggg ttggtataat tccgcaagcg gtccagactt ccggg
//
LOCUS       DTVD01000266            4322 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7264_length_4322_cov_265.565971, whole genome
            shotgun sequence.
ACCESSION   DTVD01000266 DTVD01000000
VERSION     DTVD01000266.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7264_length_4322_cov_265.565971"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            33..1712
                     /locus_tag="EYO68_09985"
     CDS             33..1712
                     /locus_tag="EYO68_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIC08167.1"
                     /translation="MKKCDLVFHYHDQTKHDYYRYAASLGHLDWDNQPDPFRRFIGSR
                     LISLPLTEVHPSLEYDQLYSQKIQAHPVSVETISALFFYSLALSAWKQYQDTSWALRV
                     NPSSGNLHPTEGYLVIDKIKGKCFLPGVYHYTPKEHGLELRTEFGLELWKELTEQLPQ
                     GSFLVGLSSIHWREAWKYGERAFRYCQHDIGHAWAALAISASMLGWKLQILNTISDEA
                     IAQLLGLNRFNDFEMEEKETPDLLAVIVPSNDTLKPSQSLKQESIQKIANGKWYGKAN
                     KLNEEYYPWEIIDMVSDACEEQKSDCDIEKPSTQLFSVTHPVPVNDVKQERKNTFLAG
                     HIIRQRRSAVAMDGVTSITKTQFYEMLSRVIPVVGSMLWDSIAQRPFIHLGLFVHRVV
                     GLIPGLYALVRDPEKQSLLQTSMHAEFQWKTPLECPQSLPLFLLEEKEVQNLAASVSC
                     GQDIAGAGAFSCGMLAEFEEPIRRFGAHWYRRLFWETGMIGQVLYLEAEAKGVQATGI
                     GCFFDNPVHDVFGLTGHAFQSLYHFTIGGSVEDDRLSTLPSYQHLASSSIL"
     gene            1746..2660
                     /locus_tag="EYO68_09990"
     CDS             1746..2660
                     /locus_tag="EYO68_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIC08168.1"
                     /translation="MPRILSLIPSSTEIVCALGFENQLIGRSHECDFPSSVQKLSSCT
                     APKFKVEGTSAEIDQRVKSILKNALSVYQIDEKKLQQMQPDIIITQAQCEVCAVSLKD
                     VENAVCEWVESRPQIVSLEPNQLSDIWKDFINIAEALGVKEQGHELVSQLKQRMNKIA
                     QKTINFPQKPTVVCIEWIEPLMSAGNWMPELIEMGGGINLFGVAGEQSPWMTWEQLLE
                     ANPDVILVMPCGFNLSRSKAEMSSLSKKPEWSQLNSVQNQQVYLTDGNQYFNRPGPRL
                     VESLEIIAEILHPAHFDFHHQQSGWEQL"
     gene            2744..4240
                     /locus_tag="EYO68_09995"
     CDS             2744..4240
                     /locus_tag="EYO68_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_385088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HIC08169.1"
                     /translation="MLIETSKKNAICFDNSYVLLGSRFYASVKPTPVERPCIVKLNYG
                     LARDLGIDLEVIEPEALAEIFSGNQILPGAEPLAMVYAGHQFGQFVPQLGDGRAILLG
                     EVIDNSSFRRDIQLKGAGLTPFSRQGDGRAALGPVLREYIISEAMHALGIPTTRSLGA
                     VTTGEPVYREKVLPGAILTRVALGHVRAGTFQYFALQRDEKALTQLADYVIDRHYPVV
                     KKVPNPYLKLLEEVMDRHARLVAQWMQVGFIHGVMNTDNMAISGETIDYGPCAFMDTF
                     DQTKVFSSIDHMGRYAYGNQPPIVQWNLTRFAETILPLIDKIPDRAVDLAKEVIEVYP
                     ERFQDYWLSGMRRKLGLFTSESEDEALVQSLLDTMQENEADFTLTFRRLCDAALGPEL
                     EGSLRYLFKDARAYDKWALNWRVRMSHEVKTPDEHVELMRQANPAVIPRNHRVEQALE
                     AAVEEGDYGPFEKLLEVLSSPYVEPVGHTEYMLPPKPEEHVLQTFCGT"
BASE COUNT         1213 a          895 c         1013 g         1201 t
ORIGIN      
        1 tcattcaata aataaaccag ggttaaaact ggatgaaaaa atgcgatctt gtatttcatt
       61 atcatgatca aacaaaacat gattattatc gttatgctgc ctctttagga catttggatt
      121 gggataatca accggatcct tttcgtcgct ttataggtag ccggctcatt tctttaccat
      181 taacagaagt tcatccttca ttggaatatg atcaacttta ttctcaaaag attcaagcac
      241 atccagtttc agtagaaacc atttcagcac tttttttcta ctcattagct ctttctgcat
      301 ggaaacaata ccaagatacc tcatgggcac ttcgtgtgaa tccatccagc gggaatcttc
      361 atccaacaga aggatatctt gtcattgaca aaattaaagg gaaatgtttc cttccgggag
      421 tttaccatta cactccaaag gagcatggat tggaactccg tactgagttt ggattggagt
      481 tgtggaaaga attgacagag caattgccac agggttcctt cctcgtggga ttatcttcaa
      541 ttcactggcg ggaagcctgg aaatatggtg agcgggcgtt tcgctattgt cagcatgaca
      601 tagggcatgc atgggcggca ttagctattt cagcttctat gctgggatgg aaactccaaa
      661 tcctaaacac catttcagat gaagcaatcg cacaattgct ggggttgaat cggtttaatg
      721 actttgaaat ggaagagaag gaaacccctg atcttttagc agtcattgtt ccttcaaatg
      781 atacactcaa accaagtcaa agtctgaaac aagaatctat tcaaaaaatt gcaaacggga
      841 aatggtatgg aaaagcaaat aaactgaacg aagaatacta tccatgggag atcatagaca
      901 tggtttctga tgcttgtgaa gaacaaaaat ctgattgtga tatagaaaaa ccaagtacgc
      961 aattgttttc agtaactcat ccggtccctg ttaatgatgt taaacaggaa agaaaaaata
     1021 cttttttagc tggtcatatt atccgtcaaa gacgtagtgc agtagcaatg gatggtgtga
     1081 cttcaatcac aaaaacacaa ttttatgaaa tgcttagtag agtgatccct gtagtgggat
     1141 ctatgttgtg ggatagtatt gctcaaaggc catttattca tttaggactt tttgttcatc
     1201 gggtggtggg attgattccc ggactatatg ctttagtgag agatccggaa aagcagtctc
     1261 tgctccaaac ttcgatgcac gctgagtttc agtggaaaac ccctctggaa tgccctcaat
     1321 cgcttcctct ttttttatta gaagaaaaag aggttcaaaa tttggctgct tctgtgagtt
     1381 gtgggcagga tattgcgggt gcgggtgcat tcagttgcgg gatgctggct gaatttgaag
     1441 aacctattcg acgctttgga gcccattggt atcggagatt attttgggaa actggaatga
     1501 ttgggcaggt tttatatttg gaagctgaag caaaaggagt acaggctaca gggattggtt
     1561 gtttctttga caatccagtc cacgatgtat ttggattaac cggtcatgct ttccaaagtt
     1621 tgtatcattt tacgatagga ggatcagtgg aagacgaccg cttgagtaca cttccaagtt
     1681 accaacacct tgcttcctca tccatcttat gatgtcatta ttctcattca caatggagtt
     1741 tcaccatgcc tcgtatcctc tccctgatcc caagcagcac tgaaattgtc tgtgctcttg
     1801 gctttgaaaa ccaactaatt ggacgatcac atgaatgtga tttcccttca tctgttcaaa
     1861 aactgtcctc gtgtacagca cctaaattta aagttgaagg taccagtgct gaaatcgacc
     1921 aacgagtcaa atccattctc aagaatgcgt tatccgttta ccagattgat gaaaaaaaac
     1981 ttcaacaaat gcaacctgac atcatcatta cccaagctca atgtgaagtt tgtgctgtca
     2041 gtctcaaaga cgtggaaaac gctgtttgcg agtgggttga atcccgccct caaatcgtat
     2101 cactggaacc gaatcaactt tcagacattt ggaaggattt tattaatatt gcagaggcac
     2161 tgggagtgaa ggaacaaggt catgagttag tttctcagtt gaaacaacgc atgaacaaga
     2221 ttgctcaaaa gacaataaat ttccctcaaa agccgacggt ggtttgtatc gaatggattg
     2281 aacctttgat gtcagcaggc aattggatgc ctgaattgat cgaaatggga ggtggaatta
     2341 atttatttgg agttgcaggg gaacagtcgc catggatgac ctgggaacaa ctgttagaag
     2401 ccaatccgga cgtcattctc gtcatgccct gtggctttaa tctatctcga tcaaaagcag
     2461 aaatgtcatc attatccaaa aaaccggagt ggtcgcagtt aaattctgtc caaaatcaac
     2521 aagtgtatct tactgatgga aaccaatact ttaatcggcc tggcccacgt ttagtagaat
     2581 cattggagat catcgcggaa atattgcatc ctgctcactt tgattttcac catcaacaat
     2641 ccggatggga acaattataa ttttttcatt catttaaaaa agctagagag gttgagcatc
     2701 agcatcgtaa attgtttcca aataatggtt gaagaggaat agaatgctaa tagagacgtc
     2761 taagaagaac gctatttgct tcgataacag ctatgtactg ctgggaagcc ggttctacgc
     2821 aagcgtaaag ccgacaccgg tggagcggcc ctgtatcgtc aaactgaatt acggtttagc
     2881 tcgggactta ggtatcgacc ttgaggtgat tgagccggag gcattggcag agattttctc
     2941 cggcaatcaa atcctgcctg gcgctgaacc tctcgctatg gtctatgccg gtcatcaatt
     3001 tggccagttc gttccgcagc tgggcgacgg gcgcgccatt ctccttggag aagtcattga
     3061 taattctagt tttcgccgtg atattcaact caaaggagca ggccttaccc cattctcccg
     3121 gcaaggagac gggcgtgctg cactcggccc agtgcttcgt gaatatatta ttagcgaggc
     3181 gatgcatgcg cttggaatac cgacaacgcg ctcgctgggc gctgtcacca ccggggagcc
     3241 ggtatatcgg gaaaaagttt tgccgggcgc gatccttaca agggtcgcct tggggcatgt
     3301 acgtgccggc acttttcaat attttgcttt gcagcgtgat gagaaggctc tcacacagct
     3361 cgccgattat gtaattgatc gacattaccc agtggtaaaa aaagtcccta acccctactt
     3421 gaaacttctg gaagaggtaa tggaccgaca tgcgcggcta gtcgcccagt ggatgcaagt
     3481 tggttttata catggcgtca tgaataccga caatatggcg atctctgggg agacgattga
     3541 ttacggacca tgtgctttta tggatacctt tgatcagaca aaggtgttta gttcgatcga
     3601 ccatatgggt agatatgctt acggcaacca gccgccgatc gtgcaatgga atctgacccg
     3661 ctttgcagaa acgatccttc cattgattga taagataccg gatcgcgctg ttgatcttgc
     3721 gaaggaagtg atcgaagtct accctgaacg atttcaagac tattggttgt cgggtatgcg
     3781 ccgaaagctt ggactgttca catcggagtc ggaagatgaa gccctggttc aatccctgct
     3841 tgataccatg caggagaatg aggccgactt cacactgacg ttccgccgac tttgtgatgc
     3901 tgctctgggt ccggaactgg aaggttcact gcgatatttg ttcaaagatg caagagccta
     3961 tgacaaatgg gccttgaatt ggcgagttcg catgtcacat gaagtcaaaa cgccggacga
     4021 gcatgttgaa ttaatgcgtc aggcaaatcc tgctgttatt cctcgtaacc atcgcgttga
     4081 gcaggctctc gaagctgccg tggaggaggg ggattatgga ccattcgaaa agctactgga
     4141 agtgctctcg tctccctacg tggaaccagt gggtcatacg gaatatatgc tgccaccgaa
     4201 accagaagag catgttctgc agacattttg cggaacttaa taagaaacgt agtggagacg
     4261 cttgtttagc aaatagtcta gtttcctatc tgattagctt gaaacttggg aagtcataaa
     4321 at
//
LOCUS       DTVD01000267            4293 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7327_length_4293_cov_456.019113, whole genome
            shotgun sequence.
ACCESSION   DTVD01000267 DTVD01000000
VERSION     DTVD01000267.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7327_length_4293_cov_456.019113"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="EYO68_10000"
     CDS             <1..499
                     /locus_tag="EYO68_10000"
                     /inference="COORDINATES: protein motif:HMM:PF03748.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HIC08170.1"
                     /translation="GGGGGGESSGGGKKKKIIIIVAVVLLLIIGIVVFLLMSGDEEEQ
                     LPEEELPVTDVAEPLAEPIFLPLESFVVNLKDGRRFLKASIQLMLSEPGAAAYLTVRL
                     VEVKDIVLAELQELSIEDVKQSDAREALRQRLISAISQIFPSKPEWEDPEPIRKVLFE
                     EFVIQ"
     gene            571..1119
                     /locus_tag="EYO68_10005"
     CDS             571..1119
                     /locus_tag="EYO68_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HIC08171.1"
                     /translation="MSDVSPIDDRQGTSMKDNLQVISINRKEFRSADKVAKKGIALFL
                     LLTALFFFVSSLNAAEEEVEEIKVIREGPPPLEDPQYLDLGTFVVNMPGDKYFLKSSI
                     QLAFENSAAKEWLLTRLPIAKDLIITHLNSITVEQFDDTKSRAVIKNDLQIRLNSLFP
                     NKAVWEDSVPIRRILFLEFYRQ"
     gene            1260..1718
                     /gene="fliN"
                     /locus_tag="EYO68_10010"
     CDS             1260..1718
                     /gene="fliN"
                     /locus_tag="EYO68_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HIC08172.1"
                     /translation="MAEEESFENLDDLDWSDVESELKANRDQILSEAKSADSGGQKEG
                     DNGDESGKENGEGSTSKTLQSSGDIDINFLFDVQLSIVVEVGRVQMLISDLLELDEES
                     IIELDSLVGQPLDIRANDLLVARGEVIVVNEKFAVRITDIVSPDNRFAAL"
     gene            1730..2956
                     /gene="fliO"
                     /locus_tag="EYO68_10015"
     CDS             1730..2956
                     /gene="fliO"
                     /locus_tag="EYO68_10015"
                     /inference="COORDINATES: protein motif:HMM:PF04347.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HIC08173.1"
                     /translation="MSFSKPTWLKAGIALALLSNWILVAAASEVRTLKNIEVVSIEST
                     EQVRLEFDGKFSGEPLINFESGSMSLRFSSTHTDPALPLLTAAKDNTLIKAVRAVEFP
                     DTHFVHLEILLQSPRTLLDHPKITHSGNYIILGFPNNFSATPALSSTNVLTKEIEKRV
                     KADQSFPSTFSKESTDEQPSFLADGLLPSPEQDWGSTMLTLVLSLLFVLFLIYLIAFI
                     YKRFFSSRFPSMQGNVHIRQVSSHHVGPKQKIIVFEMNERKFACGVTPTSINLIAELH
                     DETDPEIIHSIQTDENAKDFNIDQTGESSEIDPEQPFEQTKTNVPNNKNSKDNFDTSI
                     ELEGLEKDIFLKSNVENRNSLEESNNHNNELKSTIKTHFSKTPTKTKIMPHGNPVMQH
                     FASKLSERLKFLKPIK"
     gene            3073..3813
                     /gene="fliP"
                     /locus_tag="EYO68_10020"
     CDS             3073..3813
                     /gene="fliP"
                     /locus_tag="EYO68_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253410.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HIC08174.1"
                     /translation="MLSLLLLSVFCVGMPLYAQSPIPGINISVDTASSPQQVATTLQI
                     VFLLTILTMAPAILIMTTSFTRFVIVLSFLRQAIGTPQAPSNQIVIGLALFLSLFVMM
                     PVWEEVNEVALSPYLEETISQEEFMDRATLPIKRFMGNFTREKDLALFVRIAKIERPK
                     NIEDIPIWVMIPAFVISELKAAFQIGFLLYVPFLVIDMVIASILMAMGMMMMPPVMIS
                     LPFKLMLFVLVDGWHLILGSMVKSFVEI"
     gene            3839..4111
                     /gene="fliQ"
                     /locus_tag="EYO68_10025"
     CDS             3839..4111
                     /gene="fliQ"
                     /locus_tag="EYO68_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HIC08175.1"
                     /translation="MTEAFVVSIMIEAMRMATLLAAPMLIGALVVGLLVSIFQAVTQI
                     QEQTLAIIPKMGAMMAVLAILFPWLLTQATTYMEGIFSNLPKFIGG"
BASE COUNT         1323 a          827 c          857 g         1286 t
ORIGIN      
        1 aggaggagga ggaggaggtg aaagcagtgg cggcggaaaa aagaagaaga tcattataat
       61 cgttgctgta gtgcttttac tgatcattgg catcgtagtt tttttgctga tgtctggaga
      121 tgaagaagaa cagttaccgg aagaagaact gccggtgaca gacgtggcgg aacctcttgc
      181 cgaacctatt tttcttcctc ttgaaagttt tgtggtgaat ctcaaagacg gccgtcgttt
      241 tttaaaagcc tctatccagc tgatgctcag tgaacctggt gcagccgctt atctgacagt
      301 acgcctggtt gaggttaaag acattgtact ggccgaactg caggaattgt caatagaaga
      361 tgtcaaacaa tcagatgcca gagaggctct gcggcaacgg ttaatcagtg ccataagtca
      421 gatttttccc agcaagccgg aatgggaaga tcctgaacca ataagaaagg ttttgtttga
      481 ggaatttgta attcagtgat ccacgttaat cacagtctta atccggatac tggaaagtct
      541 ggattcatcc aggttttgaa aaaatatcga atgtctgatg tgtctccaat tgatgacagg
      601 caaggaactt cgatgaaaga taatttacag gttatttcca tcaatagaaa agaattccgg
      661 tcggctgaca aagttgcgaa gaaaggaatt gccctgtttc tgttgctgac agcccttttt
      721 ttctttgtat catctttgaa tgcagcagag gaggaagttg aggaaattaa ggtaattaga
      781 gaaggacctc ctccactgga agatcctcaa tatttggatc ttggtacttt cgtagtaaat
      841 atgcccggag acaaatattt tcttaaaagc agcatccagt tggcttttga aaattctgcc
      901 gcaaaagaat ggttgctgac aagacttcca atagcaaaag atcttataat cactcatctc
      961 aattcaatta cagttgaaca gtttgatgac actaaaagcc gggcggtcat aaaaaatgat
     1021 ttgcaaatcc gcctgaattc gttgtttccc aataaggctg tttgggagga ttctgttcca
     1081 atacgaagaa tactattttt ggaattttac cgacaatagc ataaataaga ttgcaaaaag
     1141 taaaataagc ctgaaatctg atataagttt tatcaaatga tgtttttggt tgtagaattt
     1201 cctttaaact tttagcctga aacataaaca attaaaccag tatttttaac tattgatcaa
     1261 tggcagaaga agaatctttt gaaaacctgg atgacctgga ctggtcagat gtagaaagtg
     1321 aactaaaagc taacagagac cagatcctct ctgaagcgaa atctgcagac agcggtggtc
     1381 aaaaagaagg ggataatgga gatgaatccg gaaaagaaaa tggtgaaggt agcacgtcaa
     1441 agactcttca atcgtcgggt gatattgaca tcaattttct gtttgatgta cagctttcca
     1501 ttgttgtaga agttggacgt gttcaaatgc ttatcagcga cctgttggaa ctggatgaag
     1561 agtctataat cgaattggac agtcttgtag gacaaccttt ggatatccgc gccaacgatt
     1621 tgctggttgc acgcggggaa gttattgtag tcaacgaaaa atttgctgta cggatcacgg
     1681 atatcgtcag tccggacaat cgctttgcag cactctgagg aaaaaaagca tgagtttctc
     1741 aaagccaact tggctaaaag caggaattgc gttggcgctt ttgtcaaatt ggattttagt
     1801 tgctgccgca tcagaagtgc gtactctgaa aaacattgag gttgtttcca ttgaaagtac
     1861 agaacaggtt cgcctggagt tcgatggtaa attttctgga gaaccactaa taaattttga
     1921 atcaggttca atgagcctgc gatttagttc tacacacact gatcctgcac tccccctgct
     1981 gactgctgca aaagacaata ctttaatcaa ggcagttcgt gctgttgaat ttcctgatac
     2041 acattttgtc catctggaaa ttttactcca gtctccacgg acactactgg atcatccaaa
     2101 aattacccac tccggaaact acataatcct tggttttccc aacaattttt ctgccacacc
     2161 cgcactttca agtacaaacg tcctgacaaa agaaattgaa aaacgtgtaa aagccgacca
     2221 gtctttcccc tctacttttt ccaaagaatc tacagatgaa caaccctcat ttctggcaga
     2281 tggtcttctt ccttcaccag aacaagattg gggttcaaca atgctgacac tggttttgtc
     2341 tctgttattc gttttatttc tcatttattt gatagctttt atatataaac gttttttcag
     2401 tagtcgattt ccctcaatgc agggtaatgt acacattcgt caggtttcaa gccatcatgt
     2461 tggaccaaag cagaaaatta tagttttcga aatgaatgaa agaaagttcg cctgcggtgt
     2521 aacacctaca tccatcaatt tgatcgcaga attacacgac gaaacagatc cggaaattat
     2581 ccattcgatt cagactgatg aaaatgctaa agattttaat attgatcaga caggtgaatc
     2641 ttctgaaata gatccggagc aaccctttga gcaaactaaa acgaatgtac caaataacaa
     2701 gaatagtaaa gataactttg atacttcaat tgaattagaa ggcctggaaa aagacatttt
     2761 ccttaaatct aatgttgaaa accgcaatag cctggaagag tcaaacaatc ataataacga
     2821 attaaaatca acaatcaaaa ctcatttttc aaaaactccg acaaaaacta aaattatgcc
     2881 tcatgggaat cctgtgatgc agcactttgc aagcaaactt tcagaacgtc taaaattctt
     2941 aaaacccatc aaatagagtc catgctgtat tttcaattcg aaactcaatt aactgaaaac
     3001 atggaacaag ttgcaacgtc gggctcaggc ttcagaaaat ctttttattt atgtaaaagt
     3061 ataaagtatt caattctcag cctgctgttg ctgtctgttt tttgtgtagg aatgccgctt
     3121 tatgcacaat cgccaattcc aggcatcaac atttccgttg acactgcaag ttcaccccaa
     3181 caggtagcaa ctacattgca gatcgtcttt ttactgacga ttctaactat ggcaccggct
     3241 attttaatca tgacaacttc attcacaaga tttgtgattg tgttgtcatt tttaagacaa
     3301 gctattggaa ctccgcaggc tccatcaaat caaatcgtga tcggccttgc actgtttctt
     3361 tctttgtttg tgatgatgcc ggtctgggaa gaagtcaacg aagttgccct ttcaccgtat
     3421 ctggaagaaa ctatttccca ggaagaattt atggatcgtg ccactttacc aattaaacga
     3481 ttcatgggaa attttacacg tgaaaaagat ttggctcttt ttgttcgaat tgctaaaata
     3541 gaacgaccaa aaaacatcga agatattcca atttgggtta tgattcctgc gttcgtaatt
     3601 agtgaactaa aagccgcatt tcaaattggt tttttgctat atgttccatt tcttgtgatc
     3661 gacatggtta ttgcttcaat tctgatggca atgggtatga tgatgatgcc gccagtgatg
     3721 atttctttac cgttcaaact gatgcttttt gttcttgtag acggctggca cttgattctt
     3781 ggttctatgg tcaaaagttt tgtggaaatt taattcttaa acaactaagt gagtttttat
     3841 gacagaagcc tttgttgttt ccattatgat tgaagccatg cgaatggcaa ctcttctcgc
     3901 tgcgcccatg ctgattggag cattggtagt aggtttactt gtttctatct tccaggcagt
     3961 aacgcagata caggaacaaa cattagcaat catcccaaaa atgggggcaa tgatggctgt
     4021 attggctatc ctgttcccat ggctactcac gcaggctact acctacatgg aaggtatttt
     4081 ttccaacctc cccaaattca ttggcggcta aaagtctttc aaataatccc tctctctatt
     4141 cattttaaat tcacaaaaat tcaaatcaag taatttctgg ctagaccatc tagtactgat
     4201 attggataat gtatgttgaa caaaacaaaa aactgctttt attgattaat attgactgtt
     4261 tcgtaaaaag tttgaaatcc aaccagcacc taa
//
LOCUS       DTVD01000268            4292 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7328_length_4292_cov_372.522068, whole genome
            shotgun sequence.
ACCESSION   DTVD01000268 DTVD01000000
VERSION     DTVD01000268.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7328_length_4292_cov_372.522068"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            512..3064
                     /locus_tag="EYO68_10030"
     CDS             512..3064
                     /locus_tag="EYO68_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08176.1"
                     /translation="MKIRLFLIVMIPAFLITSTIGIYLIEYILTGNKDSAFASLFSSL
                     WWTAVTFTTVGYGDMSPETVQGKIFTFFVMAAGLINFSIVVSMVTEKFQEFRSGRDRG
                     LDSIKIKSHILICSDDPTWMKEILSQNQKYVKRDKVVLISPSDEHPLLATSYKKLKWI
                     SGNSFDLNVLRKAAAAKAKIAYVFFKDSSYALMTVLQLETLSDGRIVTQAQYVGREYR
                     KYFEDVGCDHALDPYDLYVPMMLSAFHSQGAPAWINKVINRNHGHQIATKKTDSLHIG
                     KTWLELIKTNKQNHGIMPLAVVINEVVLINPDASFEIPKDSLIMQLEPFEISKVSSVM
                     QFDVTESSPKGDLEKNAIDVMGMDEIGIDGHILISSDNQVFINRCLLEMSQRNQPEKI
                     IVLSDTPLMEEIPDNLDVEWIEGDSNSEKSFLEARSSEAKVALIDHADDGQNLMAVLR
                     LEQATDGEVFTIATYHKEDFDQQLFKVGCDFCLEPEELIAPILSQSALNPGLGTLIEE
                     IISEEPTTQSLHVRQLSREWEEDSWLSTILNLKEKEGELPVGLICCQTHKLLVNPHPE
                     LQVKPGDRLIYIASAPIRAKQNGDVLKPVEDSESTEEDVKPSEEAEKLYRQGLTLFKK
                     DENYEEAYSCFHQAAILNHTRAKYNLGLMNYNGKGVPKNLDESYHWFHEAAKFGNENA
                     RKALKSTRALRDITSNTMEHEIPEFDNDLIGRMPEEQLYWFASAVIAMVMADEHIDLH
                     ERSFLHSAIRIVRDKRKIQELEEYILRWQTPPIGAMDFSTKDRDHILESLLNIAAVDR
                     NFDEREEKLLREIAGLMKVPEQQIENLIELGHKRVEQFRANQLRAPNVRARL"
     gene            complement(3118..>4292)
                     /locus_tag="EYO68_10035"
     CDS             complement(3118..>4292)
                     /locus_tag="EYO68_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HIC08177.1"
                     /translation="LMILSKVVLKPVVRYLLRSPELLFVSALGYCMGIASICGFIGIS
                     PEAGAFFAGISVGNLPYRLDIEDKVGPLKLFGQALFFLTLGVEIATIQTENFSGNMGA
                     IIPLVILVVFIKPFFLIITGYATKLKGRTAFFIGTTLNQSSAVSIIVALLAWKYNLFD
                     DRLFAILITVILLSLLISAMGHAVQPGLYNSFKWLVGVLNRNISALDLENNQQVVLKD
                     HVVLLGFNEIAQEIGEFYEEQLTPERVLLIDCDPEIIKHFEARKDSNVDPVYADPNDP
                     AVWREYKLSEAKVVVSCTGSDLDSDLELAGYIRHTAPDLPFLAVTTSHEDSLKLYESG
                     VRYVVQTDQLASKTFRDIFAEEIDKPASESFVEEGRSHWKDTRSIRDGLGDIFKLV"
BASE COUNT         1349 a          819 c          908 g         1216 t
ORIGIN      
        1 gtctatcagc cctgatattc agtactattc agtaacactt gatataagtg acaagtggag
       61 ttagaatact gaatatataa gttaatgtta cttggtggta ttcagtataa tacgctttgc
      121 acggactcat aatcgtccgg tcgcaggttc aagtcctgcc tggcccacca ataaatacat
      181 tgattcgcct tcagacatct ttagaatttc cattgtgtct gtctatttga ccacaattcc
      241 agtctctcca ctttattcta ctcttttatt tactcactct tcttaaggaa ctgattaaaa
      301 cactctgaag cagtattttt actgtgtccc aaaatttgca tgtaaaaaac gtattatttg
      361 caaaaggact tgcaaatttg gtaatattgc tctgttaatg aacaaggaag tttaatcatc
      421 cagattgaga attggccccg agttacctgc ttcctgaaac attattagtc ataatccatg
      481 agaaaaaaat aatctgctga taaaatttaa aatgaaaatt cgtttatttt tgattgtgat
      541 gattccggct tttctgatta cttctacaat cggaatatat ttaattgagt acattctgac
      601 tggaaataaa gatagcgcct tcgcttcact attcagctct ctctggtgga cagcagttac
      661 atttactacg gtgggctatg gagacatgtc gccggaaact gttcagggga aaatttttac
      721 tttttttgta atggcggcag gtttaatcaa tttttcaatt gttgtttcaa tggtgacaga
      781 aaagtttcag gagtttcgtt ccgggcgtga tcgcggactg gattccatta agataaaaag
      841 ccatattttg atatgttctg atgatccaac ctggatgaag gaaattctct ctcagaatca
      901 gaaatatgta aagagagaca aggttgtatt gatttctcct tctgatgaac atccgctgtt
      961 ggctacgtca tacaaaaaat taaaatggat ctcaggtaat tcttttgatc taaacgtttt
     1021 aaggaaggca gcagcagcaa aagcaaaaat tgcctatgta ttttttaagg acagcagtta
     1081 tgccctgatg acagttttgc agcttgaaac tttgagtgac ggtaggattg tgactcaggc
     1141 acaatatgtc ggccgggaat atcggaagta ttttgaagat gtcggttgcg accatgcttt
     1201 ggatccatat gatctctacg ttcctatgat gctgtcagct tttcattcac aaggtgctcc
     1261 ggcatggatc aataaagtga tcaatcgcaa ccatggacat caaattgcca ctaaaaaaac
     1321 tgattcttta catattggta aaacctggct ggagctgata aaaacgaata aacagaacca
     1381 tggaattatg cctcttgcag ttgttataaa tgaagtagta cttatcaatc ctgatgcttc
     1441 atttgaaatt ccgaaagaca gtttaataat gcagctggaa ccgtttgaaa tttcaaaagt
     1501 cagttcggta atgcaatttg atgtcactga gtcaagtcca aaaggcgatc ttgaaaaaaa
     1561 tgctattgat gtgatgggaa tggatgaaat aggtatcgac ggacacattt taattagctc
     1621 cgataatcag gtgttcatta atcgatgttt gctagaaatg agtcaaagaa atcaacctga
     1681 gaagataatc gtgctttcag atactccgtt aatggaggaa attcctgata acttggatgt
     1741 cgaatggatt gaaggagatt caaactctga aaaatcgttt ctggaagcca gatcatcaga
     1801 ggcgaaagtt gccctgattg atcatgcgga tgatggtcaa aacctgatgg ctgtcctgcg
     1861 tctggaacag gcaactgatg gagaagtgtt taccattgca acctatcaca aagaagactt
     1921 tgatcaacaa cttttcaagg ttggctgtga tttctgtctg gaaccggaag agttaattgc
     1981 gcccatcctt tcccagtcag cattaaatcc cggattaggt acattgattg aagaaattat
     2041 ttcggaggaa cctactaccc agagtctgca tgtccgccaa ttaagtcggg aatgggaaga
     2101 agacagttgg ctttcaacta ttttaaattt gaaggaaaaa gagggcgaac tgcctgtagg
     2161 actgatctgc tgtcagactc acaaactcct ggttaatcct catcctgagc tgcaggtaaa
     2221 gcctggagac cgtttgattt atattgcctc agcccctatt agggctaagc aaaatggaga
     2281 tgttctcaaa cctgttgagg attcagaaag tacagaggaa gacgttaaac cgtctgaaga
     2341 agcagaaaaa ttataccgtc aaggactaac gttattcaaa aaagacgaaa attatgagga
     2401 agcgtacagt tgtttccatc aggcggcaat tctgaaccat acacgagcaa aatataatct
     2461 tggtttgatg aattataatg gtaaaggtgt accaaagaac ttggatgaat catatcattg
     2521 gttccatgaa gcagctaaat tcggtaatga gaatgcccgt aaagcactga aaagtaccag
     2581 ggctttacga gatataacat caaatacaat ggaacatgaa attcctgagt ttgacaatga
     2641 tttgataggc cgaatgcccg aagaacaact gtattggttt gccagtgcag taattgcaat
     2701 ggtgatggca gacgagcata ttgatcttca tgaacgctct ttcctccact cagctattcg
     2761 aatagttcgg gataaaagaa aaattcagga gcttgaagag tatattctac gttggcagac
     2821 accgccgatt ggagcaatgg atttcagtac aaaagatcga gatcatattc tcgagagtct
     2881 gctcaatata gctgcggttg acagaaattt tgatgagagg gaagaaaagt tattgcgtga
     2941 aatagcagga ttgatgaaag tacctgagca gcaaatagaa aacctgatag aacttggtca
     3001 caagcgggtg gaacagttca gggcgaacca actgcgtgct ccaaatgtga gggcaagact
     3061 atgatataaa agagtattaa gtccgtaaaa accgtacata cttttatccg gaaaaaatca
     3121 gaccagcttg aatatatctc ccaatccatc cctgatcgag cgggtatcct tccagtgact
     3181 tctgccttct tccacaaaag actcagaagc aggcttgtct atttcttccg caaagatatc
     3241 tctgaaggtt tttgatgcaa gctgatcagt ctgaacaaca taacggaccc cgctctcgta
     3301 gagtttcagt gaatcctcat gagaggttgt gacagctaaa aacggaagat caggtgctgt
     3361 atgcctaatg taacctgcga gttccagatc ggaatctaag tcacttccag tacaggaaac
     3421 aacaactttt gcctcactaa gtttatattc tctccacaca gccggatcgt taggatccgc
     3481 atagaccgga tcaacgttgg aatctttccg tgcctcaaaa tgtttaataa tttctggatc
     3541 gcagtcgatc aataaaacgc gttctggagt aagttgctcc tcataaaact ctcctatctc
     3601 ctgagctatt tcgttaaaac ccagcagtac aacatggtct ttcagtacga cttgctgatt
     3661 gttttccagg tctaaggctg aaatatttcg gtttaataca ccaacaagcc atttgaaaga
     3721 attatatagt cccggttgaa ctgcatgacc catggctgag ataaggagtg aaagaagaat
     3781 tactgtaatt aaaatcgcaa aaagacgatc gtcaaagaga ttatatttcc aggccaaaag
     3841 ggcgacaatg attgaaactg ctgatgactg atttaaggtc gtcccgatga aaaaggcggt
     3901 gcgtcctttg agctttgttg catacccggt aattattaaa aagaaaggtt tgatgaaaac
     3961 aactaaaatc acgagcggga tgattgcccc catgtttcct gaaaagtttt cggtttgaat
     4021 tgttgctatt tccacaccaa gcgtcaggaa aaataatgct tgtccaaaca atttgagagg
     4081 accaaccttg tcctcaatgt caagcctgta cggcaggttc cctactgaaa tacctgcgaa
     4141 gaaagctcct gcttccggag aaattccgat aaaaccgcaa atggacgcaa tgcccatgca
     4201 atatcccaaa gcgctgacaa acaaaagttc tggggagcgt aataaatagc gcacaactgg
     4261 tttcagtact acttttgaca ggatcattag aa
//
LOCUS       DTVD01000269            4276 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7370_length_4276_cov_684.925610, whole genome
            shotgun sequence.
ACCESSION   DTVD01000269 DTVD01000000
VERSION     DTVD01000269.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_4276_cov_684.925610"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            52..873
                     /locus_tag="EYO68_10040"
     CDS             52..873
                     /locus_tag="EYO68_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC08178.1"
                     /translation="MSSNQPELHLQQFSKILNLLSNGKKLESIWYGPPPEEAPTLLFL
                     HEGLGCVSMWRNFPEKLAQQTGCGAMVYSRLGYGNSDACELPRSLNFMHPEGLEVLPE
                     IIEQCKINKCILVGHSDGASIALIYAGENSTESLLGIVTEAPHVFCEQLTIRSINKVG
                     ELFRSGNLRELLKKHHGSNIDCAFKGWLEVWLHPDFAEWNIESCLPKIMVPQLVIQGN
                     DDEYGSSAQVEAIVRQSGSIVETRFLDNCGHSPHRDQETQTLEFMTQFVRNLLDR"
     gene            1016..1474
                     /gene="aroQ"
                     /locus_tag="EYO68_10045"
     CDS             1016..1474
                     /gene="aroQ"
                     /locus_tag="EYO68_10045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIC08179.1"
                     /translation="MPTVLLLNGPNLNLLGTREPEIYGSMNLSGIEKQVSAVLHEHKI
                     ECETFQSNSEGELIDWLHQHKNADFLLLNPGALTHTSIGLRDAVLGIEIPFLEIHLSN
                     VHQREEFRHHSFFSDIAVGSLVGLGVKGYLLAAQFAVDFIEQKGTSAGNR"
     gene            1584..2549
                     /locus_tag="EYO68_10050"
     CDS             1584..2549
                     /locus_tag="EYO68_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspC"
                     /protein_id="HIC08180.1"
                     /translation="MGGSLLSTWLQKEVLSISVSPPESYQIQTEKLPIDSGKSIKSFE
                     VILERNIFNAQKTEIELPPLLPEPEIIAPETTDEEAVEHTRLAIALAGTMIYGGKSSF
                     AFISKKDSLHKYVIYGIGECFDAKTILREKECGSESVKVLDIRDRWVLILHKETKQAL
                     WMVSPVAVEEEVSAAVQPKNVQTKTKTTQKTTALVVPPKEKAVTKNSALPSIPAEGEN
                     TFHFQRVWVDEQLENFGQLLNDARIVPTMKDEKPYFMFQFIKEQSIYEKLGLKQNDII
                     LEINGFLVDTVGKALRLLEVLQSEREISLKVEREGEPVQFHYYID"
     gene            2594..4129
                     /locus_tag="EYO68_10055"
     CDS             2594..4129
                     /locus_tag="EYO68_10055"
                     /inference="COORDINATES: protein motif:HMM:PF03772.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIC08181.1"
                     /translation="MQFKWHKTGLIELTFLLAALIWISFRYQDTLNLLHPEWIILGLG
                     LGFLWCLRHKLYLPLFLLLLIAGTLLHQWFVVLPNSLKWLQEEISAEEAIMLNGRIQE
                     RIVENDTIRLQLNSVDVIKGEQKQWLPEAILSFPENSRRISSFYRGRKLQVIGRVDHV
                     SIKKNRLNLAFSEFRYRNAISPQTKLSRKRNSWVRQLTDRAEYHLSNQALALYLPLTL
                     AKRSYDSRSTAKIFQETGMAHLLAISGLHIGLIYGIILGLVRWLGRLSNRFLEHPRFY
                     GITHLISLTGIWLYLLLIGMPTPAFRAVCMLSLLVAGRLLGQVHHPLYALFATAFFFI
                     FLNTAVIYEVSFQLSFIAVFFILWFLPLYPHPHENDVFWKRIFKYGLISFAITSAVML
                     GTWPVVASIFGRISLETFWLNLIMVPLLGLFVLPCCLLTLVVSALSLGNPPFGILENG
                     VFSLTELVVEGWFAVLQNLHHWGDWASFPLTLDWGPPQFILYYATILGFGKLLTDRFR
                     KIS"
BASE COUNT         1198 a          888 c          922 g         1268 t
ORIGIN      
        1 ttttactggc aagcagtgta gcagccattg ctttttttct tcccggacaa cgtgtcctcc
       61 aaccagcctg agctgcatct ccaacaattt tcaaaaattt tgaatctctt atcaaacggc
      121 aaaaaactgg aatcaatctg gtatggtcca cctccggaag aagccccgac gctgttattt
      181 cttcatgaag ggctaggctg tgtttcaatg tggcggaact ttcctgaaaa actggcccaa
      241 caaaccggat gcggtgccat ggtatacagc aggcttggtt acggtaattc ggatgcgtgt
      301 gaacttcctc gttccttaaa ttttatgcat ccggagggat tggaagtact tccggaaatt
      361 atagagcaat gtaaaatcaa caaatgcatt ttagtgggtc actcagatgg agcttcgatt
      421 gctttaattt atgcaggaga aaactctacc gaatctttac ttggaatagt tacagaggca
      481 ccacacgttt tttgtgaaca gctcacgatt cgttccatta ataaagtggg tgagctgttt
      541 cgctccggga atctccggga acttctcaaa aaacatcatg gaagtaatat tgactgtgct
      601 ttcaaaggct ggttggaagt ctggcttcat cccgattttg cagaatggaa catcgaatcg
      661 tgtttaccga aaattatggt tccgcaatta gtcatccagg gtaacgacga cgaatacgga
      721 agctctgccc aggtcgaggc gattgtccgt caatccggaa gtatagtaga aacacgcttt
      781 ctggacaact gtggtcattc tccgcatcgg gatcaggaaa cacaaacttt ggaattcatg
      841 acccaattcg tccggaactt gcttgataga tagaaaacac cggaaacttg tttgccaatt
      901 cacttgcgtt ttttcagcac aggctttagt tttagcagat aaatattagt tccggactgc
      961 tcaaatagaa aataagtatc cagcacattc aattaattca gctgatttcc aaactatgcc
     1021 taccgtactt ctgctaaatg gtccaaacct taatttactg ggaactcgtg aaccggaaat
     1081 atacggttca atgaatttaa gtggaattga aaaacaagtt tcagccgtac ttcatgaaca
     1141 caaaatcgaa tgtgaaacct tccagtcaaa ttcagaagga gaacttattg actggcttca
     1201 tcagcacaag aatgcagatt ttttgctttt gaatccggga gcactgaccc acaccagtat
     1261 tgggctgcgg gatgccgtgc tgggtataga aataccattt ctggaaatcc acctttccaa
     1321 cgttcatcag cgggaggaat ttcggcatca ctcttttttt tcagacatcg cagttgggtc
     1381 attggttggg cttggagtta aaggatattt gctggcggcc caatttgccg tagattttat
     1441 tgagcagaaa ggaacatctg caggcaacag atgatcctat tattcaccat ccggccttcc
     1501 cgattttact gcctgttatg agaatttcct caaccaccat atttttatca ttcaacgttt
     1561 ttcttgccgg agttgcctcg tacatgggag gctcacttct gagtacgtgg ctacagaagg
     1621 aggtcttgtc gatatccgtt tcacctccag aatcttacca gattcaaacg gagaagctac
     1681 ccatagattc gggtaaatcc ataaaatctt ttgaagtaat tttggagcgc aatattttta
     1741 atgcccaaaa aacggaaata gaattaccac ccctgcttcc ggaaccagaa ataatagctc
     1801 cggaaactac agatgaagaa gcagtcgaac atacccgatt ggctatagca cttgcaggaa
     1861 caatgatcta tgggggaaaa agctcctttg cattcattag caagaaagac agtctgcaca
     1921 aatatgtcat atatggaatt ggtgaatgtt ttgatgcaaa gacaattctg cgtgaaaaag
     1981 aatgtggttc tgagagtgta aaagtacttg atatcaggga tcgctgggtt ttgattcttc
     2041 acaaggagac aaaacaggcc ctttggatgg taagtccagt tgcagtagag gaagaagtat
     2101 ctgctgcagt tcaaccaaag aatgttcaaa ctaaaactaa aacgacgcaa aaaactactg
     2161 cccttgtggt tccaccaaaa gaaaaagctg ttactaaaaa ttctgcttta ccgtcaattc
     2221 cggctgaagg tgaaaatact tttcattttc agcgggtctg ggtagatgaa caactcgaaa
     2281 attttgggca gctgctgaat gacgccagaa ttgttcctac aatgaaagat gaaaaacctt
     2341 atttcatgtt tcagttcatc aaagaacaaa gtatctatga aaaattggga ttgaaacaaa
     2401 acgatatcat tttagaaatt aacggctttc tggtggacac agtaggcaag gcgctcaggt
     2461 tactggaagt gcttcaatct gaaagggaaa tttcactaaa ggttgaacgg gaaggggaac
     2521 ctgtgcaatt tcattactac attgattgag catcagggct gaaaatagca cactataaag
     2581 cgcattttgc agaatgcagt tcaaatggca caaaaccggt ttgatcgaat tgactttttt
     2641 actggctgcg ctgatttgga tttccttccg ttaccaggac acacttaatt tgcttcaccc
     2701 ggaatggatt attctgggtc ttggccttgg ttttttatgg tgtctgcgtc ataaattata
     2761 tcttccactc tttttgcttc ttttgatcgc aggtaccttg ctgcaccaat ggtttgttgt
     2821 acttccaaac agcctgaaat ggctgcagga agagatttct gcagaagaag caattatgct
     2881 gaatggaagg attcaggagc gcatagttga aaatgacacc atccgtctgc aactgaattc
     2941 agttgatgtc atcaaaggtg aacaaaaaca atggttaccg gaagcaatct tgagctttcc
     3001 ggaaaacagc cgcagaattt caagttttta tcgtggacgt aagctgcagg taattggcag
     3061 ggtggatcat gtcagcataa aaaaaaatcg tcttaattta gctttttcag aatttcggta
     3121 tcgtaacgca atttctccgc aaacaaagct ctcccgtaaa cgaaacagtt gggtaaggca
     3181 attgacagac agggccgaat atcatctttc caatcaggcg cttgcgcttt atttaccgct
     3241 gactttagcc aagcgttcat acgattcacg ttccaccgca aagatttttc aggaaaccgg
     3301 aatggcacac ctcctggcta tcagcggact gcatatcggc ttaatctacg gaattatact
     3361 aggacttgtt cgctggctcg gtcgcctgtc aaaccgattt ttggaacatc cccgttttta
     3421 tggtataacc cacctgattt ctctgacagg tatttggctc tatctgttgt taattggcat
     3481 gcccactccg gcattccgtg cggtttgtat gctgagctta ctggttgccg gtcgattact
     3541 cggtcaagtt catcaccctt tgtatgccct ttttgccaca gcattctttt ttattttcct
     3601 taatacagca gttatttacg aagtctcatt tcagctctca ttcattgccg ttttttttat
     3661 attatggttc ctgcctcttt atccacatcc tcacgaaaat gatgtatttt ggaaacggat
     3721 atttaaatac ggcttgatca gttttgccat tacaagcgca gtcatgctcg gcacctggcc
     3781 tgtggtggcc agtatttttg gacgtatttc actggaaaca ttctggctaa atctgatcat
     3841 ggtgccgctt cttggtctat ttgttttgcc ttgctgcctg ctgactttgg tggtgagcgc
     3901 attgtctttg ggtaatcctc cattcggaat tttggaaaac ggtgtttttt cgctgactga
     3961 gcttgttgta gagggttggt ttgcagttct gcaaaacctg catcactggg gtgactgggc
     4021 atcgtttccg cttactttag actggggacc acctcaattt attctgtatt acgctaccat
     4081 tttgggcttt gggaaattac tgacagaccg attccgaaaa attagctaat aactatgtaa
     4141 atcattgtaa tgcaagtaac ttgtgcgcaa taatatccag gctatgaatc taaatttaat
     4201 agaaaagttt ctgtatgaat aagttaccaa aaactatgaa aggggtctgg ctaactggcc
     4261 atggtgaact agacaa
//
LOCUS       DTVD01000270            4238 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7463_length_4238_cov_158.531198, whole genome
            shotgun sequence.
ACCESSION   DTVD01000270 DTVD01000000
VERSION     DTVD01000270.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_4238_cov_158.531198"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(63..890)
                     /gene="iolB"
                     /locus_tag="EYO68_10060"
     CDS             complement(63..890)
                     /gene="iolB"
                     /locus_tag="EYO68_10060"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIC08182.1"
                     /translation="MTSLLSRVQPINSEGRIQNITPESAGWKYVGFDVFKLKSGQSLS
                     LETADREVCLVLVTGKADIKSRDESWDNLGERMSVFAEAPGEEWKPPFAVYVPNDDRF
                     EIEALTDLELAVCSAPGKGNYPARLISPDDMTYETRGDGTNIRHICNILPETAKADNL
                     LVVEVITPNGSWSSYPPHKHDSDNLPEESSLEEIYYFHVDPPQGFAFQRVYTDDGSLD
                     ETMTVHNQDVVQVPRGYHPVGAPHGYDLYYLNVMAGPKRIWKFYNDPEHEWIVKKDE"
     gene            complement(901..2862)
                     /gene="iolC"
                     /locus_tag="EYO68_10065"
     CDS             complement(901..2862)
                     /gene="iolC"
                     /locus_tag="EYO68_10065"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIC08183.1"
                     /translation="MSNTSNLIDNSRPLDLICMGRVAVDLYAEQVHSPLEDAQSFRKY
                     LGGCAGNTAVGTARLGLKSAMFSCVGADDMGVYLRNVLTNEGVDTSLLRNTPEHLTAL
                     VLLGVSPPDRFPLIFYRENCADMQILPSDANPEIFKNAKALLITGTGLSTPSMREATR
                     QAVKVAKESGCAVIFDIDYRPVLWGMTDAGDGETRFVASKTVTQELQPFLAELDLIVG
                     TEEEILIAGSESYETETLESCLAAIRKQSSATVVLKRGAEGCEVFSPESSTPISARSF
                     PIEVLNVLGAGDAFMSGFLRGWLRNENLETCALYGNACGALVVTRHGCSPASPSFAEI
                     EYFISNFDNFSTMAQHPHQTFWSKMNQLHLRTELRQPQNPERPVREELLILAYDHRTQ
                     FEDSCRENDLPLDLISTFKEQVYKGFQKVHEANKNKGLAILIDPEYGQTILNNSADAD
                     YVIGVPIEKAGAFPLSWLKDGSLYQQLLERPAGWLVKVLWHFHTQMSPEEKEVQLSQL
                     RKLNEVCTALKRKLMIELIIPEDFPETGSSLGETMSEVYQAGISPFWWKITALDTEKE
                     WQTMTATLDKYDPDVGVIILGKNAPIEQFKTWFSVARSTPHTCGFAIGRSIFWEAWEQ
                     FAEGKINKSEVSEMIAERYQQVIDIWHNI"
     gene            complement(2876..3880)
                     /gene="iolG"
                     /locus_tag="EYO68_10070"
     CDS             complement(2876..3880)
                     /gene="iolG"
                     /locus_tag="EYO68_10070"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIC08184.1"
                     /translation="MKPVTFGIIGAGRIGKLHADNLLSRVDGARLKAITDPYLDEEWA
                     AFRNIPLTGKDHRILLDDPEIDAILIGSPSAEHATQMIECAEAGKHIFCEKPIALDPN
                     VIRNALTKVDKSGVKFQGGFNRRFDPNFSAVQQQVASGALEDPHIVRITSRDPAPPPA
                     EYVAGSRGMFLDMTIHDFDMTRFLSGSEVTEVHAYGAVLVDPEIGKAGDIDTAVISLK
                     FANGALGIIENSRKAVYGYDQRVEFFGSKGAAMADNNTPTSMVVFNESGTIRDKPLYF
                     FLERYEEAFVAEMQAFVNAIREDKPTTVSGNDGLAPVLIALAAQESLKSGKPEKVDSV
                     "
     gene            complement(3904..>4238)
                     /locus_tag="EYO68_10075"
     CDS             complement(3904..>4238)
                     /locus_tag="EYO68_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HIC08185.1"
                     /translation="SSDRIANLLQASSAFGKTMESVDRTKMTKLIYQVLLARKKYHQI
                     SPDETQAVWASVIDGYSFPTNLDLDSPVEGLVTENIWTLMERALKEEWPVDRFNKCLN
                     EATIRRHP"
BASE COUNT         1103 a         1022 c          921 g         1192 t
ORIGIN      
        1 agtactaata ttttaatcct gttcgcattt ctttttttaa cagaatgtgt acaacgcttt
       61 cattattcat ctttcttgac gatccattca tgttcaggat cattataaaa tttccaaatc
      121 cgttttgggc ccgccatcac attcaggtaa tacagatcat atccatgcgg cgcaccgact
      181 ggatgataac cacgagggac ctgaacaaca tcctgattgt gaaccgtcat ggtttcatcc
      241 aggcttccat catctgtata aacacgctga aaagcaaaac cctggggtgg atcaacatga
      301 aaataatata tttcttccaa tgatgattct tccggtaaat tgtctgaatc atgcttgtgc
      361 ggcgggtaac tcgaccagct tccgtttggt gtaattactt ctaccaccag cagattatct
      421 gcttttgccg tttccggaag aatattacag atgtgacgaa tgttggttcc atctcctctt
      481 gtttcgtagg tcatatcatc cggagaaatt aaacgagccg gataattccc tttccccgga
      541 gcagagcata ctgccagttc caggtcagta agcgcttcta tttcaaaccg atcatcattg
      601 ggaacataaa cggcgaaggg cggtttccat tcttcgccgg gagcttcggc aaaaacgctc
      661 atacgttcgc ccaaattatc ccatgattca tcacgggatt ttatatcggc tttgccggtg
      721 acaagcacca ggcaaacttc gcggtcagct gtttccagtg ataatgattg tccggatttg
      781 agtttaaaaa catcgaaacc cacatatttc cagccggcag attccggagt gatattttgg
      841 atgcgccctt cagaattgat cggctgtacg cgtgaaagta aagaagtcat aatatgtcag
      901 ttaaatgttg tgccaaatat caatcacctg ttggtaacgt tctgcgatca tctcagatac
      961 ttcagacttg ttaatttttc cctctgcaaa ctgctcccat gcttcccaga aaatactgcg
     1021 cccgatggcg aatccacaag tatgcggtgt tgaacgggct acgctaaacc atgttttgaa
     1081 ttgttcaatc ggagcatttt ttccgagaat aattactcca acatccggat cgtatttatc
     1141 cagagttgcg gtcatagttt gccactcttt ttctgtgtcg agagcagtga ttttccacca
     1201 aaatggggaa attccggctt gataaacttc agacattgtt tctcccaaag atgatccggt
     1261 ttctggaaaa tcttccggaa taatcaattc gatcatcagt ttgcgtttga gtgctgtgca
     1321 gacttcgttt aattttcgga gttggctgag ttgtacttct ttttcttccg gactcatttg
     1381 tgtatgaaaa tgccacaaaa ccttcacaag ccaacctgcc ggtcgttcca ggagttgttg
     1441 ataaagtgag ccatccttta accaactcag tggaaatgct ccagcttttt caattggtac
     1501 accgataacg taatctgcat cagcagaatt attgaggatg gtttgaccat attccggatc
     1561 aattaaaatg gcgagtcctt tatttttgtt agcttcatgg actttttgaa aacctttata
     1621 aacttgttct ttgaatgttg aaatcaaatc caacggcaaa tcattttcgc ggcaactgtc
     1681 ttcaaattgt gtgcggtggt cgtaagccaa aatcaacaat tcttcccgca ccggacgttc
     1741 cggattctgc ggttgtcgta attcagttcg caaatgcagt tgattcattt tagaccagaa
     1801 cgtctggtgc ggatgctgcg ccatagttga aaaattgtca aagttgctga taaaatattc
     1861 tatctctgca aaagacggcg aagccggaga acatccgtga cgcgtaacca ccaacgctcc
     1921 acaggcattt ccgtaaagtg cgcaagtttc cagattttcg ttccgcaacc aaccccgcaa
     1981 aaatccgctc ataaacgcat ctcccgcacc caaaacattc agcacctcaa tcggaaaaga
     2041 gcgggcggaa atcggtgtgg aggattccgg agaaaaaacc tcacaacctt ccgcaccacg
     2101 cttgagtacg actgtagcgg atgattgttt ccgaatggca gccaaacagc tttccagtgt
     2161 ttcagtttcg taagactcgc ttccggcaat taaaatttct tcttcggtac cgacaatcaa
     2221 atcgagttct gcaagaaacg gctggagttc ctgcgtcaca gtttttgagg caacaaaacg
     2281 ggtttcaccg tctcccgcat ctgtcatgcc ccacaaaacc ggacgataat caatgtcaaa
     2341 aattacagca catccggatt ctttagccac cttcacggct tgccgagtgg cctcccgcat
     2401 cgatggtgtg gacaatccgg ttccggtaat caacaaggct ttggcgtttt tgaaaatttc
     2461 cggatttgca tcgctgggaa gaatctgcat atcggcgcaa ttttcccgat aaaagatcaa
     2521 ggggaaacga tccggaggac tcacgcccag caaaaccaat gcagtcaaat gttccggagt
     2581 atttcgcaaa agagaagtgt ccacgccttc atttgtcaaa acattccgta aataaacacc
     2641 catatcgtcc gcaccgacac aggaaaacat agctgatttc agtcccaaac gggccgtgcc
     2701 gacagcggta tttccagcac atccacccaa atatttgcga aaactttgcg cgtcttccag
     2761 cggactgtgg acttgttcgg cgtataaatc cacagccact cggcccatgc aaatcaggtc
     2821 aagcggtctg gaattatcta ttaagtttga tgtatttgac atatttttag gtgaattaaa
     2881 cagaatcaac tttctctggt tttccgctct taagagattc ctgtgccgcg agcgcaatca
     2941 ggaccggagc caggccgtca tttccggaaa cggtagtagg tttgtcttca cggattgcat
     3001 tgacgaaggc ctgcatttca gcgacaaatg cctcttcgta gcgttccaga aaaaagtaaa
     3061 gcggtttgtc ccgtattgtt ccggattcgt tgaaaacgac catcgaagta ggcgtgttat
     3121 tgtctgccat cgcggcgcct tttgaaccga aaaattcgac acgctggtca taaccgtaaa
     3181 ccgctttgcg gctgttttca ataatgccaa gtgcgccgtt tgcgaatttc agtgaaataa
     3241 ccgcagtatc aatgtcgccc gccttgccga tttccgggtc aaccagaacc gcgccgtaag
     3301 cgtgaacttc ggttacttca cttccgctga gaaagcgagt catgtcgaag tcgtgaatcg
     3361 tcatgtccag aaacattcct ctggaaccgg ctacatactc ggccggcggt ggagcgggat
     3421 cgcgtgaagt aatacggacg atgtgcggat cttccaaagc tccggaagca acctgctgct
     3481 gtactgcaga aaaatttgga tcaaaacggc gattgaaacc cccctggaat ttgactccgg
     3541 atttgtcaac ttttgtcaag gcattccgga ttacgttcgg atcgagagca atcggttttt
     3601 cacaaaaaat atgctttccg gcttcggcgc attctatcat ttgcgtggca tgttcagcag
     3661 atggtgagcc gatcaggatt gcgtcaattt ccggatcgtc gagcaatatc ctgtgatctt
     3721 ttccggtcag cggtatattc cggaaagcgg cccattcttc gtctagatac ggatctgtga
     3781 ttgcttttaa gcgcgctcca tcgacgcggc tgagcagatt gtccgcatgt agtttgccga
     3841 tgcggcctgc tccgatgatt ccaaaagtta caggtttcat ttaatcctca ttattttttt
     3901 cattcaggga tgtctccgta ttgtagcttc attgagacac ttattaaaac gatcgaccgg
     3961 ccactcctct ttcaaggccc gttccatcaa agtccatatg ttctctgtaa cgagaccttc
     4021 cacgggagaa tccaaatcca ggttggtcgg aaaagagtag ccatcaataa ctgatgccca
     4081 aacggcttgt gtttcatctg gactaatttg atggtatttt tttctagcaa gcagtacttg
     4141 atagatcaac tttgtcatct tggtacggtc cacagattcc atcgtcttac cgaaagcact
     4201 ggaggcctgt aataggttgg caatacggtc gctagatt
//
LOCUS       DTVD01000271            4222 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7514_length_4222_cov_398.822174, whole genome
            shotgun sequence.
ACCESSION   DTVD01000271 DTVD01000000
VERSION     DTVD01000271.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_4222_cov_398.822174"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="EYO68_10080"
     CDS             complement(<1..136)
                     /locus_tag="EYO68_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HIC08186.1"
                     /translation="MSVNISDVLDIVDSQNQWRGKEAINLIASENVQSDAVKQIESND
                     F"
     gene            complement(178..1572)
                     /locus_tag="EYO68_10085"
     CDS             complement(178..1572)
                     /locus_tag="EYO68_10085"
                     /inference="COORDINATES: protein motif:HMM:PF01467.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HIC08187.1"
                     /translation="MSEGDLRQSLEKIKSPRILVVGDLMLDYYSWGKVDRISPEAPIP
                     VMQVLREDRRLGGAGNVVMNLTTLGAEVLVCGVTGKDETGECIRKLLAENGVDHSGVI
                     ISENYKSCLKHRMIAGQTHLLRMDIDPDPEKLVPQKQLTDYLEQTIPKSHAVIVSDYG
                     KGLLSTESLKTIAACGKKHGVPIVGDPRRTTNYSIYQDFTLIKPNRKETEAAVGFALK
                     DQNDVLKAAEQLKAEVNVEYLVISLDRDGLLLFHNPEDYNFLEAETQEVFDVVGAGDM
                     VSSMLTFMLAGQAKIEQAAYWAQLAASLEIQHVGVVSFTKNELLQRFDYGETSAKIVT
                     QEKLYRNLPQEIPIVFTNGYFDEISAGHLKFLHQLNTLKGFNVVAINSDRSITKQKGH
                     PPLLNERERALLLSAIEAVDRVIIFDDADASSLIRNIRPTIVVKGKHFEKQLLPEQEA
                     IRESGAKLEYFPEY"
     gene            complement(1578..2615)
                     /locus_tag="EYO68_10090"
     CDS             complement(1578..2615)
                     /locus_tag="EYO68_10090"
                     /inference="COORDINATES: protein motif:HMM:PF13462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08188.1"
                     /translation="MITAKLFKYLISFILIFTAYFTAIAQTSSAAPAGTVFHPNPVLG
                     LVDGKPVTFEDVRNKKANDLSLQLYQHLSVRLMEYALEKLAAKHQEIILTPEKKVTLK
                     DIIAVYEQNDLQERGTLEQIRPQIKKFLEQQIRSQHMLNQYSLALKKGWVISNLEAPS
                     DFLLKGNIKTGYLRGNKKASVILLEYSDYQCPFCGRVQSTIRKLLDKYQDRVAFGYRH
                     FPLAFHKEADEAAIASECAREQGKFEEIHRILYSRQKAQDKEELKKYAREIKVKYPAK
                     FDECLDSEKYRGLVDQDMKDGANLGITGTPGFFVGLFNPKSGEIQGEVLSGAQPYDAF
                     QQALEKYLSQN"
     gene            complement(2632..3270)
                     /locus_tag="EYO68_10095"
     CDS             complement(2632..3270)
                     /locus_tag="EYO68_10095"
                     /inference="COORDINATES: protein motif:HMM:PF02190.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08189.1"
                     /translation="MTTVKLIDTIPLFPLHKTILLPGSVLPLHIFESRYRQMVEHVLE
                     HEDLLIGIIQPATQDSEKLCEIGCAGKIEQSQQLPKGNYLIQLHGESRFKIVEELETD
                     TPYRQAKVEYSSFPNDTEDLRLSKDSELLYQNFREYSERNKLKIEWEKIEDIPANYLV
                     NLLSMNLDFSGIEKQTLLESPDLDSRLDDLICLMGMSNPNEILTDFSPNFLN"
BASE COUNT         1115 a          944 c          864 g         1299 t
ORIGIN      
        1 tgaaatcgtt tgactctatt tgtttgactg catccgactg cacgttttcg ctggcaatca
       61 ggttgatggc ttctttgccg cgccattgat tctggctgtc gacgatgtcc aggacatcag
      121 aaatatttac actcattttt tccttgaaga ttaattaaaa aaggggagac tgattcctca
      181 atattccgga aaatactcca gtttggcacc agactcacga atggcctcct gttccggaag
      241 aagttgtttt tcgaaatgtt taccttttac aacaattgtc ggacggatat tgcggattaa
      301 gctgcttgca tccgcatcat caaaaataat aacccggtcc acagcttcaa tagcggagag
      361 caacagggcg cgctcccttt catttagcaa aggtgggtgt cctttttgtt tggtgatgct
      421 cctgtcacta ttgatagcta cgacattaaa gcctttaaga gtattgagtt gatgcaggaa
      481 tttcaggtgc cctgcagaaa tttcgtcaaa atatccattt gtgaaaacaa tcggaatttc
      541 ttgcggcaag ttgcggtaca gtttttcctg agtcacgatt ttcgcagaag tttctccgta
      601 atcaaaacgc tgcagcaatt cattttttgt aaatgaaacg acacctacat gttgtatttc
      661 caaacttgca gcaagttgag cccaataagc ggcctgctca atctttgcct gtccggcaag
      721 cataaacgtg agcatgctgc tgaccatgtc tccggctcca acaacatcaa aaacttcctg
      781 agtttctgct tcaaggaagt tataatcctc tggattgtgg aaaagtagta aaccgtcccg
      841 atcaaggctg ataaccagat attccacatt tacttcagct ttcaactgct ctgcagcctt
      901 gaggacatca ttttggtctt taagagcaaa tccgactgct gcttcagttt ccttgcggtt
      961 tggtttaatg agtgtgaaat cctgatagat gctgtagttt gtcgtacgtc gaggatcacc
     1021 aacaatcgga actccgtgtt tttttccaca agccgcaatt gtttttagac tttcagtact
     1081 gagtaaaccc tttccgtagt cagaaacaat gactgcgtgg ctttttggaa ttgtttgttc
     1141 caaataatca gttagctgtt tttgcggtac aagcttttcc ggatccggat caatatccat
     1201 ccggagcaaa tgggtttgtc cggcaatcat acggtgcttg aggcagcttt tataattttc
     1261 agaaatgatt acacctgaat gatcaacacc gttttccgca agcagtttac ggatgcattc
     1321 gccggtttca tcttttccgg tgactccgca aacgagaact tctgcaccca aagtggtcag
     1381 attcatcact acattacctg ccccgcctaa gcgccggtct tcccggagca cctgcattac
     1441 aggaattggc gcctccggag atattctatc cactttaccc cacgaataat agtccagcat
     1501 caggtctcct acaacgagga tgcgtggaga ttttattttc tccagagatt ggcggaggtc
     1561 tccttcgctc ataatgatta gttttggctt aagtattttt ctaatgcctg ttggaaagca
     1621 tcatagggtt gtgcgccgga aagtacttca ccctgaattt ctccggactt tggattgaag
     1681 aggccaacaa aaaaacctgg agttccggta atgccaagat tagctccatc tttcatgtcc
     1741 tgatcaacta gtccacggta tttctcgcta tcgaggcact cgtcgaattt agctgggtat
     1801 tttactttta tttctctggc atattttttc agttcttcct tatcttgtgc cttttgacgt
     1861 gagtagagta tcctgtgaat ttcttcaaat ttgccctgtt cccgggcaca ttccgacgca
     1921 atagccgctt cgtcggcttc tttgtgaaaa gcaagcggga agtgccggta tccaaaagcc
     1981 actcggtcct ggtatttatc gaggagttta cggattgtac tctgtacacg accgcaaaaa
     2041 ggacactggt aatccgaata ttcaagcaga atgaccgagg cttttttgtt gcccctgaga
     2101 taaccggttt ttatattacc tttcagcaga aaatctgaag gggcctccaa atttgaaata
     2161 acccagccct tcttaagtgc cagtgagtat tggttcaaca tatgctgact cctaatctgc
     2221 tgctccagaa actttttaat ctgcggccgg atctgttcca aggttcctcg ttcctgcaga
     2281 tcattttgtt cataaacagc aataatatct ttgagtgtca cttttttttc tggagtcaag
     2341 attatttcct gatgttttgc agccaatttt tccagagcat attccatcaa ccgtacactc
     2401 agatgctgat acagctgcag actaaggtca tttgcttttt tgttgcgcac atcttcaaaa
     2461 gtcaccggtt ttccatcaac caagcctaaa accgggttgg gatgaaagac tgtacctgca
     2521 ggagcagcac tggaagtttg tgcaattgca gtaaaataag cggtaaaaat gaggatgaat
     2581 gagattagat acttgaataa ttttgcagtt atcatgtaat ttatctctta tttaatttag
     2641 aaagttgggt gaaaaatcgg ttaaaatttc attggggttc gacattccca tcagacagat
     2701 caagtcatcc aaacgggagt ccaggtctgg agactccaac agtgtttgtt tttctattcc
     2761 ggaaaaatcc aggttcatgc tgagtaaatt aacgaggtag tttgcaggaa tatcttctat
     2821 tttttcccac tctattttca gcttatttct ttcagaatat tccctgaagt tttgatacaa
     2881 aagttctgaa tccttagaaa gcctcaaatc ttctgtgtca tttggaaatg atgaatactc
     2941 tacttttgcc tgtctgtatg gagtgtctgt ttccagttcc tccacaatct taaagcgaga
     3001 ttctccgtgg agttgtatta gataattccc ttttggcaat tgttgagact gttcaatttt
     3061 tcctgcacag ccgatttcac acagcttttc agaatcctgg gtcgcaggct ggattatccc
     3121 aatcagcaag tcttcatgct ccaggacgtg ttccaccatc tgtcggtaac gcgactcgaa
     3181 tatatgcaga ggcagaactg aacccggcaa taaaatcgtt ttatgcagag ggaacaaagg
     3241 aatagtatcg atcagtttta cagtagtcat tagacctcaa ttggaaatcg ttgctgcaga
     3301 ctgcctgcaa tccgactttc aacatctact gcagtttgag gttgatgcgg catagtattt
     3361 ttccagactg aactattttc catacaaaga tatgttggaa tttgaggtgc caatttttca
     3421 attctcagct gaacattccg gaacaaacgc tgacgtattt tttttaaata acgcattttc
     3481 ccatcctctc cagggacaaa ctctccaaga aaaacacggc tgtttggaaa gcgttcatcc
     3541 acaactgatt tgagcgaaga aatgtagcgt agagaaccca aactgaccca tgcaatacca
     3601 tcagaaggca cggcatcaag tatttgctca accagttcct gataaccttt ttcccaatcc
     3661 ggatagtgga tcatgggatc aaaatgaaaa gcgactttgt accctgccgc tgaagccttt
     3721 cctgctgctt caagccgttc tttcagccgc gctgttttgt gttcttcctg ttcaacaatg
     3781 gcttccggat ttaccgacca tgaaataacg gtttttccac cgtgcggaag atcgagcaaa
     3841 tgatcaacat tacttgattt cgttttcagt tcaagcaggg cgttcggtag ttttgcaaaa
     3901 aaacggatta catgcgcctt gatacccaga atgtggtcga gtgccaaact gtcagaatgc
     3961 tctcctgttc ctacacgaaa tagagtattc ggctgttccg cagtacgctg ccaaacctgc
     4021 tccagcattt catccagatt tgcgtgaacc gtaataacag gtttgttcag gaatgcttgt
     4081 agaatacaat atgtacactc aaatggacag ttttctatta aatccagcgt aaaataattg
     4141 cagcagacaa gatcattcga aaaaccagga caagccttaa tcgatgttcc cttgaaatac
     4201 ttgagatgca aggtcttttt tc
//
LOCUS       DTVD01000272            4207 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7560_length_4207_cov_249.901975, whole genome
            shotgun sequence.
ACCESSION   DTVD01000272 DTVD01000000
VERSION     DTVD01000272.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7560_length_4207_cov_249.901975"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="EYO68_10100"
     CDS             complement(<1..1179)
                     /locus_tag="EYO68_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HIC08190.1"
                     /translation="MSIDTTNIPEQIIALKHRIHEQCPNITEHFRELETILAEEIDSI
                     ESASKRGESVIPEIAFSDIVASQVDDTTIKAVKRRGAVVVRGVFTKKKASWWYEELER
                     YLETNGYFEQDDPELDHYFSDLKADRPQICAVYWSKPQVEARQSPNLAQTRSFLNRLW
                     NYQENGTQYFDPDRECTYVDRVRMRQPGDTSLGLSPHIDGGSIERWLGDNYQQAYRSL
                     FSSNWKSYNPFNGAFRPEVEGIDSPAVCRAFRTWQGWTALTKQGPGDGTLQLIPTTLS
                     IDYVMLRPFQEDVPEDMLCGAVEGRAQAITDEWHSLLLRGLVSIPKVNPGDTVWWHPD
                     VVHAVEHEHQGSNVSSVLYIGSAPWCDRNVRYLERQTQPFLEGRSAPDFAPEDYELAY
                     T"
     gene            complement(1283..2383)
                     /gene="ugpC"
                     /locus_tag="EYO68_10105"
     CDS             complement(1283..2383)
                     /gene="ugpC"
                     /locus_tag="EYO68_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HIC08191.1"
                     /translation="MATLKVSNLTKNFGNTQVLKQINLEVIDGEFIVLLGPSGCGKST
                     LLNIIAGLETVDDGEILIDEYVVNRVEPKDRNIAMVFQSYALYPAMTVRDNVIFGLKQ
                     RKVSKEHIEKSLKHFSQMLQIDQLLDRKPAQLSGGQRQRVAMGRALVREPQIFLFDEP
                     LSNLDAKLRVEMRHEINKLHEQLGTTMIYVTHDQVEAMSLATRIAVMNEGIIQQIGTP
                     QEIYEKPNSVFVADFIGSPAINLIQGTFYKDGTAPSFIPEQQKDNFKISLSGYSFDSE
                     PKDGQEIIFGFRPEHISLPDASLKSQLPVELKPSLIELTGYEKEVSFEFYGNEITGRL
                     PRHVNTELGKSISLSLDLSEISIFDSASTMRI"
     gene            complement(2432..3313)
                     /locus_tag="EYO68_10110"
     CDS             complement(2432..3313)
                     /locus_tag="EYO68_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIC08192.1"
                     /translation="MENIERSASRKRTFYRWLLFITLGIFALYYLAPLYVMIITSLKS
                     MEEIRQGNLMTLPGEIIFDSWKTAWSGRGGIDAGNVFLRPFFWNSIKMVVPAVAISTF
                     FGALNGYALTKWRFRGDNLVFLLFLFGCFIPYQAILLPMARTLGVLGISNSITGLVVV
                     HSIYGLSFTTLFFRNYYVSIPDEMIKAARIDGAGFFKIFFKVLLPISAPIIVVTVIWQ
                     FTNIWNDFLFGSTFSFGEAAPIQVALNNMVLTSTSVKEYNVDMAAAFIAGIPTLLVYV
                     LAGKYFIRGLTAGSVKG"
     gene            complement(3324..4202)
                     /locus_tag="EYO68_10115"
     CDS             complement(3324..4202)
                     /locus_tag="EYO68_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIC08193.1"
                     /translation="MFYWLQKNLPKIVISPSFAVILWFVYGFVLWTFYISLTKSKMLP
                     RYEFWGIDQYFRLWSNPRWFIAVENLLIFTVLFVGICILIGILLSILLDQKIRAEGFL
                     RTVYLYPMALSFIVTGTAWKWMLNPTMGIEKLFTDWGFTDFTFDWLVNRDMSIYTIVI
                     AAVWQSSGFVMALFLAGLRSIDDEIVKAAKIDGASLYSIYITIILPMMRPVFMSSIVI
                     LLHISIKSYDLVIALTAGGPGISSDMPAVFMTQMAFHRSEIGLASASAVMMFLTVLAV
                     VVPYLYSELRRQDANA"
BASE COUNT         1173 a          943 c          961 g         1130 t
ORIGIN      
        1 tgtataggca agctcataat cttccggagc aaaatcagga gcgcttcgtc cttccagaaa
       61 cggttgcgtc tggcgttcca ggtaacgcac gttcctgtca caccacggcg cagagccgat
      121 gtagagtaca ctgcttacat tggaaccctg atgttcgtgt tccacggcat ggaccacatc
      181 cgggtgccac cataccgtat cgcccggatt tactttgggg atcgaaacca gtccacgcag
      241 gagaagagaa tgccactcat cggtaatggc ttgcgcacga ccttcgactg ctccacaaag
      301 catatcttcc ggtacatcct cctgaaacgg gcgcagcata acatagtcaa tgctgagagt
      361 ggtgggaata agctgcaggg ttccgtctcc cggaccctgt ttagtgagtg cagtccagcc
      421 ctgccaggtc cgaaatgccc ggcatactgc aggagagtca attccttcca cctccggtcg
      481 gaacgctcca ttaaaaggat tgtaactctt ccaattgctg ctgaacaatg agcggtacgc
      541 ctgctgatag ttgtctccca gccagcgttc gatggatccg ccgtcaatgt gaggtgaaag
      601 tcccagtgat gtatcaccgg gttgcctcat tcgtacacgg tccacatagg tgcattcacg
      661 atccggatca aaatactggg tgccgttttc ctgataattc catagccgat tcagaaaaga
      721 ccttgtttga gccagattcg gactttgccg tgcttccacc tgaggttttg accagtacac
      781 tgcacatatt tgaggccggt ctgccttcag atcgctgaaa tagtgatcca gttctgggtc
      841 atcttgctcg aaatacccat tggtttcaag atatctttca agttcttcat accaccatga
      901 tgcctttttt ttagtaaaaa caccgcgaac caccacggct ccccggcgtt tgacagcctt
      961 aatcgtagta tcatcaactt ggcttgcgac aatgtctgag aaagctattt caggaataac
     1021 ggattcaccg cgtttgctgg cggattcaat ggaatcaatt tcttctgcaa gtatggtctc
     1081 tagctccctg aagtgttccg ttatgttcgg gcattgttca tgaatacgat gtttcagtgc
     1141 gataatctgc tcaggaatgt tagttgtgtc gatactcatg taaaaccaag cttaaagaat
     1201 gcgtattccg atctgaggat caaagtatac ggaatggtaa atgttgaggc tcagcgtaat
     1261 tttgctacag ttttcagtga tgtcaaatgc gcatggtgct tgcgctgtca aagatcgaga
     1321 tttcagacag gtcaaggctc agtgagatgg acttgccaag ttcggtattt acatggcggg
     1381 ggaggcgtcc ggtgatttcg tttccataaa attcaaagct aacttctttt tcatatcccg
     1441 taagttctat cagtgaaggc ttgagttcaa ccggcaattg agactttaga gaagcatccg
     1501 gaagactgat atgttcaggc cgaaaaccaa aaatgatttc ctgtccatct tttggttctg
     1561 aatcaaaaga ataaccgctg agagaaattt taaaattgtc cttttgctgt tcgggaatga
     1621 aactgggtgc agttccatct ttgtaaaatg taccctgaat caggtttatc gcaggagaac
     1681 caataaagtc cgcaacaaaa acactgttag gtttctcata aatttcctga ggagttccaa
     1741 tctgctggat gataccttcg ttcatcactg ctattcgagt agcaaggctc atggcttcga
     1801 cttgatcgtg agtcacataa atcattgtcg ttcccaactg ttcgtgcagt ttgttgattt
     1861 cgtgacgcat ttctacgcga agttttgcat caagatttga cagtggctca tcgaagagaa
     1921 aaatttgagg ttcacgcacc agtgcccgtc ccattgcgac acgctgtcgc tgccctccgg
     1981 aaagttgtgc cggtttgcgg tctaagagtt ggtctatctg cagcatttgt gaaaaatgct
     2041 tgagcgattt ttctatatgc tctttagaca ctttacgctg tttcagtccg aaaataacat
     2101 tgtctcggac tgtcatggct ggatataggg catatgactg gaagaccatg gcaatgttac
     2161 gatctttcgg ttcaacacgg ttaaccacat attcatcaat cagaatctct ccatcgtcta
     2221 cagtttcgag tcctgctatg atattgagca gcgttgattt tccacagcct gaaggtccga
     2281 gcaggacgat aaattcacca tcaattacct ctagatttat ttgcttgagt acctgtgtgt
     2341 taccgaaatt cttggtcaga ttactgactt tcaaagttgc cattttttcc ttaaatattg
     2401 ataagcttgc tgactttaaa tattgtcatt cttagccttt tactgaacct gcagtgagtc
     2461 cacggatgaa gtatttgcca gccagaacat aaaccagcag agttggtatt ccggcaatga
     2521 atgctgcagc catgtccacg ttgtattctt tgacgctggt actggtgaga accatattgt
     2581 tgagtgctac ctgtatcggg gctgcttcac caaaggagaa agtagaacca aagagaaaat
     2641 cgttccaaat atttgtaaat tgccatatca ctgtaacgac tatgattggc gcagaaattg
     2701 gtagcagaac cttgaagaaa attttgaaaa aacctgcacc atcaatcctg gcagccttta
     2761 tcatttcgtc gggaatcgaa acgtagtaat tacggaagaa tagggtagtg aaactcaaac
     2821 cgtaaatcga atgtacgact acaagtccgg tgatagaatt tgagatccct aaaacaccta
     2881 gggttctggc cattggcagc aggattgcct ggtagggaat aaaacaaccg aagaggaaca
     2941 ataggaacac gaggttgtcg cctctaaaac gccacttggt aagagcataa ccattcaatg
     3001 caccaaagaa agttgaaatc gcgactgcag gtactaccat ttttattgag ttccagaaga
     3061 aggggcgaag aaagacgttg ccggcatcaa ttccaccgcg accactccat gcggtcttcc
     3121 atgaatcgaa tattatctct ccgggtaaag tcatgaggtt accttgcctt atttcttcca
     3181 tgctctttag cgaagtgata atcatcacat aaagtggtgc aagatagtat aaggcgaaga
     3241 ttccgagcgt tataaaaagg agccagcgat aaaaggtccg tttacgggaa gcggaacgtt
     3301 caatgttttc catcttttga aaatcaagcg tttgcatcct gcctcctcag ttctgaatac
     3361 aggtaaggta caacaacggc cagcactgtt aggaacatca ttactgcact tgctgaagcc
     3421 agaccgattt cactgcggtg gaaagccatt tgggtcatga agaccgcggg catgtcagaa
     3481 gatataccgg gacctcccgc agtcaatgcg atcaccaggt cataactctt tatcgagata
     3541 tgaagcagaa ttacaatgga actcatgaaa acggggcgca tcattggaag tatgatggtg
     3601 atgtaaatac tgtagagact ggctccatcg attttagccg ccttgactat ttcatcgtca
     3661 attgaacgaa gtccggccag aaagagggcc attacgaacc ctgaggactg ccagacagct
     3721 gcaatcacga tagtgtaaat cgacatgtca cggtttacaa gccagtcaaa agtaaagtct
     3781 gtgaatcccc agtccgtgaa gagcttttca atgcccatag ttgggttaag catccacttc
     3841 catgcagttc ctgttactat aaaggagagt gccattgggt agaggtagac tgtccttaaa
     3901 aaaccttctg cccggatctt ttgatccagt aaaattgaaa gtagaatgcc gatcaggata
     3961 caaatgccaa cgaagaggac ggtaaaaatc agcaggttct ccactgcaat gaaccaacgc
     4021 ggattagacc agagacggaa gtactggtct atcccccaga attcgtagcg aggtaacatc
     4081 tttgactttg taagcgaaat ataaaaagtc cagaggacga aaccatagac aaaccacaga
     4141 atcactgcaa atgaaggcga gataacaatt tttggaaggt ttttctgtag ccagtaaaac
     4201 atttaaa
//
LOCUS       DTVD01000273            4205 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7562_length_4205_cov_273.272771, whole genome
            shotgun sequence.
ACCESSION   DTVD01000273 DTVD01000000
VERSION     DTVD01000273.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_4205_cov_273.272771"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(238..1716)
                     /locus_tag="EYO68_10120"
     CDS             complement(238..1716)
                     /locus_tag="EYO68_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HIC08194.1"
                     /translation="MRLILKIFIFLLLLTSPLSVNSAEPEVLYLWQISSGHEWESYGD
                     ENVQQKYEGITSNGEPDGKGILSFPDGKRVTGEWKNGNEWNTDHFNNEGKLIGRTAKG
                     KYIIGAGEKIKMVLFLRKVSGRWGWHEEGVAGKDYKYEGTIENGKPNGSGIYNTPSGN
                     QYIGDFKDGKKHGRGIFTYTSGSTYQGEFKDGKKHGQGTFTSPNGNRYVGQWEKSKKN
                     GQGTFTYASGSMYQGEFKDDKQHGQGTFTWKNGAKRVGEFRKGKLFNITEFGKNEKMI
                     KKWVHGVMVVDKKKEKLLFLHNENGKWIWLESGNEKYDGKYVGSINKKGIPGGAGTLT
                     FPDGNKYEGEWKDGERNGRGTYTYNDGNQYIGDFKSGKKHGQGLFTFPNGNKYEGEWK
                     EEKRHGQGSYTWANGEKYVGEFKDGKFNGQGSFTFPEGGELEGHKYEGEWKDDKKNGQ
                     GTYFFPDGGKLVGEFRKDSPWNITDYDKNGKIKGKYVNGVRQ"
     gene            complement(1941..2723)
                     /locus_tag="EYO68_10125"
     CDS             complement(1941..2723)
                     /locus_tag="EYO68_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIC08195.1"
                     /translation="MPLFGLGVWAAQSGKETYDAVLSALNAGYRHIDTAEMYGNEKDV
                     GAAVNDSGLKREEVFVTTKLWDSGLGYDHALKAFDVSLQKMNLDYVDLYLIHWPEKGS
                     QLKIWRALERIKKEGRSRSIGVSNFAPRHLKELLVEGSEHPVVNQIELSPFLQQEHIY
                     SFCQKENIHLTGYCPLSRGNRFDDPNLCRIAKEMKKSAAQVMIRWALQRGHTVIPKSV
                     SPERIGENANVFDFNLNNEQMKILDGLEEGLRFCPDPAVTQI"
     gene            complement(2889..4115)
                     /locus_tag="EYO68_10130"
     CDS             complement(2889..4115)
                     /locus_tag="EYO68_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC08196.1"
                     /translation="MRFDALSSGPYRRFWLGSIASVGSTQLYFVGMAWLVFELSDSAL
                     DLGLLGAATAVPTILATLVGGLVADRINRRSVLILTTGTSAILLLILAVLDATELVRV
                     WHVLLISALLGLVQGFDFPSRSSIFPALIEPKQMMSAVALNSILWQGSRMIFPAIGGL
                     LIALTDTSLIFVMCGIGFITMVMVLFSLEVVQEIPEKADPWHEFKEGVRFVLHQRLFL
                     TLIMLTWISMFFGTSFVQIMPIFADILHSGERGYGLLISATGLGSVAGNLFISHFQQS
                     RRLGLLMLSCAALAPFSLAGFSLVTGTLANAAGAFWLACFFAVLASAFSSVFLVSSMT
                     VLQLKVPDNLRGRVMGIHSITFSMIALGGLASGALATKFSAPVAVVIGAVVVLSSVIW
                     VTIRQGEIINLDGSQL"
BASE COUNT         1155 a         1002 c          755 g         1293 t
ORIGIN      
        1 gggtgtcatt gtatttttga aacattgggt tctcctaatg attggttgtg aatagtgatc
       61 ttcaaatgcg agaataaaat tctcaacagc actcgtcaag aaaagtattc aggtgccaag
      121 aaatgtgtgg tgtgattgtg tgaaataaga ctgcgttgta agatcgttaa acaagcgggg
      181 gagttaataa tcacgaaggt gaaaatccgg acaggtaccc cggatatccc gggtttctta
      241 ctgtcttacc ccattaacat actttccttt gatcttgccg tttttatcgt aatcagtaat
      301 gttccatggt gaatctttcc tgaattctcc cactaattta ccgccgtcag gaaaaaagta
      361 tgttccctgt ccgttttttt tatcatcttt ccattctcct tcatacttgt gtccttctaa
      421 ttctcctccc tcagggaaag tgaatgatcc ctgcccgtta aattttccat ctttaaactc
      481 tccgacatac ttctctccgt tagcccaggt atatgaacct tgaccatgcc tcttttcttc
      541 cttccattct ccttcatatt tgtttccatt tggaaaagtg aacagacctt gcccgtgttt
      601 tttcccactc ttgaaatctc ctatatactg gttaccgtca ttatacgtgt atgttcctcg
      661 accatttctt tctccatcct tccattcacc ctcatatttg tttccatcag gaaaagtgag
      721 agttcccgct ccacctggaa ttcctttctt attaattgat ccgacatatt ttccatcata
      781 tttctcattt ccactttcaa gccagatcca ttttccattc tcattatgaa gaaacaagag
      841 cttttctttt ttcttatcaa caaccattac tccatggacc cactttttaa tcattttttc
      901 gtttttgccg aattcagtaa tattaaaaag tttacctttt ctgaattcac ctactctttt
      961 tgctccattt ttccaagtga aagttccctg tccatgttgt ttgtcatcct tgaattcacc
     1021 ttgatacata gatccactgg cataagtgaa agtcccctgt ccattttttt tgcttttttc
     1081 ccactgtcct acataccggt tcccattagg cgaagtaaaa gttccttgtc catgtttttt
     1141 cccgtccttg aattcacctt gatacgtaga tccactggta tatgtgaaaa tccccctccc
     1201 atgttttttc ccatccttga aatcgcctat atattggttg ccactgggtg tattatatat
     1261 tcctgaacca ttcggtttcc cattttcaat tgttccttcg tacttataat cctttcctgc
     1321 aacaccttcc tcatgccaac cccatctacc gctcaccttt cgaagaaaca gtaccatttt
     1381 tattttctca cctgctccta taatatactt cccttttgca gtccttccta tgagtttccc
     1441 ttcattgttg aaatggtcgg tgttccactc gttcccattt ttccactcac cagtcactct
     1501 tttaccatca ggaaaagata atattccctt tccatcaggt tccccgtttg aggttattcc
     1561 ttcgtatttt tgttgaacat tttcatctcc ataactctcc cattcatgac cggaagatat
     1621 ttgccatagg tacaacactt ctggttctgc cgaattaact gacagaggtg aagtcaagag
     1681 aaggaggaag ataaagattt ttagtataag tctcatgggt ttctatttgt tggttagcag
     1741 aattggaaat atatcatcat taggtatttt ttacaagaaa gggctttccc ggggttgagg
     1801 tggcctgtgc aaactctctt taacataata taacatcatt tggtatattt tcaagaaatg
     1861 gtaattttaa cttgagcata ttttgtttta aagaatttag actttcaagg agtaactttc
     1921 caaaataaag ttataacatc ctaaatttgt gttactgcgg ggtccggaca aaaacgtaat
     1981 ccttcttcca gtccatccaa gattttcatt tgctcattat tcaaattgaa atcaaataca
     2041 ttagcgtttt ctccgattct ttccggactt acagatttcg ggataactgt atgtccacgc
     2101 tgcaaagccc agcgaatcat cacctgagca gcagattttt tcatttcttt tgcaatccga
     2161 caaagattgg gatcatcaaa acgatttcct cttgaaagcg gacaataacc ggtcaggtga
     2221 atgttttctt tttggcaaaa tgagtaaatg tgttcctgct gtaaaaaagg actcaactct
     2281 atctggttta cgaccggatg ttcactgcct tcgactagca attctttcag gtgtcttgga
     2341 gcaaaattac tgacaccgat agagcggctt cgtccttctt ttttgattct ttctaaggcc
     2401 ctccaaattt taagctgaga tcctttttca ggccaatgaa tcaggtaaag atccacataa
     2461 tctagattca tcttttgcaa gctgacatca aatgctttca aggcatgatc atagcctaaa
     2521 ccactgtccc acaacttagt tgtaacaaag acctcttctc tttttaaacc cgaatcgtta
     2581 acggcagcac caacatcttt ttcatttcca tacatttctg cagtgtcaat atgccggtat
     2641 cctgcgttta aagcagacaa aacggcatca taagtctctt ttcctgactg ggcagcccag
     2701 actccgagtc caaacaatgg catgctgtta ccgtcctgga gtgtaatacg gctgctgata
     2761 ttcaaaggtg agaacacttg gatagactta ttttcgatta tactgtttgg gtgataactt
     2821 gcttcgaatt taactcatct gcaaatattt tccaaatatt aaagttattg atctgatttt
     2881 gtgcatgttc atagttgact gccatcaaga ttgattattt ctccctgtcg aatcgtcacc
     2941 cagattactg atgacagcac taccaccgct cctataacca cggcaaccgg ggctgagaat
     3001 tttgtagcca gggctccgga agcaagtccg ccgagtgcaa tcatgctgaa agtgatgctg
     3061 tgaattccca tcacgcgacc acgcaaatta tcgggtactt tgagctgtaa aactgtcatg
     3121 gagctaacca gaaagactga actaaaggct gaagccaaaa ccgcgaaaaa gcaggcaagc
     3181 caaaatgccc cggccgcatt ggccaatgtc cctgtaacca gactgaaacc tgccagtgag
     3241 aatggtgcca acgctgcgca agataacatc agcaacccca gtcgacggga ttgctgaaag
     3301 tgagatatga acaagttacc ggctactgaa ccgagcccgg ttgccgaaat caataaacca
     3361 tatccgcgtt ctccactatg taatatgtca gcaaagatcg gcatgatctg tacaaaagat
     3421 gttccaaaaa acatggaaat ccaggttaac attatcaggg tcaggaacaa acgttgatga
     3481 aggacaaacc tcactccctc tttaaactca tgccaggggt cagctttttc cggaatttcc
     3541 tgcactacct caagtgaaaa cagtaccata accattgtta tgaaaccaat cccacacatg
     3601 acaaagatta gtgaggtgtc ggtcagtgca atgagcagtc ctccaattgc cggaaaaatc
     3661 atacgtgatc cctgccaaag gatagaattt aatgcaacgg cgctcatcat ctgttttggt
     3721 tcaatcagtg ctggaaaaat cgaagagcgc gacggaaaat caaaaccctg taccaatcca
     3781 agcagggctg aaattaagag aacgtgccaa acccgcacta gctcagtggc atccagtacc
     3841 gccagtataa gcagcagtat agcagaagtc ccagtggtca aaattaaaac tgagcggcgg
     3901 tttattcgat ctgcaaccag accaccaacc agagttgcaa gaatggtggg tacggcagtt
     3961 gctgcaccaa gcaggccgag atcgagtgca gaatcgctga gttcgaaaac aagccatgcc
     4021 atccctacaa aataaagctg cgtactacct actgaggcga tggatccaag ccagaaacgt
     4081 ctgtaaggac cagatgaaag cgcatcaaat cgcacgcaga actcctccag tatcccatgg
     4141 gaatcttcga tatacctttg aactcacagc ggattcagtc ttgatttcga ctcgatcgac
     4201 tatac
//
LOCUS       DTVD01000274            4182 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7641_length_4182_cov_412.440998, whole genome
            shotgun sequence.
ACCESSION   DTVD01000274 DTVD01000000
VERSION     DTVD01000274.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_4182_cov_412.440998"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            169..2427
                     /gene="rnr"
                     /locus_tag="EYO68_10135"
     CDS             169..2427
                     /gene="rnr"
                     /locus_tag="EYO68_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIC08197.1"
                     /translation="MKSNKKAILRMLEKQGDSLSVKQIRGSLGLEKAATAKLKAELLK
                     LIKAGKIAKHGTRYQKSQEITERTGSKAKIKGNREKVKNELIWKQTPERRQRRSSGKT
                     ETGYFTRNPKGFGFVAIGGGRPDVFIGEHEQGFAMEGDLVEIEVFRKRGFRSKQKGEI
                     IKVLERASREILARLKRTKRETVAVPVHRNSGLPFLRIAPKDDLPELESGTLVEVELL
                     EEKKQTKKTFPAPCGRVLRALENTSDHELGFQLILNENLIRTEYPAEALAHVNNFSQQ
                     VRFDASSGRRDLRDLDFVTIDGKSARDFDDAVCVIPDKDSSGGFRLFVAIADVAHYVQ
                     PEDPVDQEALLRGTSVYFPTHAVPMLPEGLSNNLCSLRPQVNRLTLTCEMRITAEGEV
                     VGYSLSESVIRSRARLIYEDVADLLEGRTSSIRDPELQTNIRKMHKLAKILERKRIQR
                     GAVQFSFAEEVFEFDADQQMTGVGRIYQSSAMKLIEQFMLEANETVARHCVKNKMPAL
                     YRVHERPDMRKLQKLQQTFFRFGVRTPLAKLADPRQFNAVIEQIQELPHFDQLQVLLL
                     RTMALAVYQTTNKGHFGLAAEYYAHFTSPIRRYPDLVVHRAIKDMLHQDQGLRTGKRT
                     LPQVNSEMAEQCSQQERRAEKAERQSIDLMKVDFLAPHAGQTFQAVVISVDSQGFRVN
                     LEPHGLEWFLPLDSMHDDSYIFDEERLSLQGRRKNRTLQAGQRLEIRLLRADPIHRIL
                     EFDVERWLSNKY"
     gene            2456..>4182
                     /gene="typA"
                     /locus_tag="EYO68_10140"
     CDS             2456..>4182
                     /gene="typA"
                     /locus_tag="EYO68_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIC08198.1"
                     /translation="MENTISNNRIRNLAIIAHVDHGKTTLVDQLFRQSGLFRENQSVD
                     ERIMDSMDLERERGITIAAKNCSVTWNETKINILDTPGHADFGGEVERALMMVDGVIL
                     LVDASEGPLPQTRFVLKKALEGQKKVLVVVNKIDRQDARVAEVLDEIYDLFIDLDANE
                     EQLEFPVLYAIGKDGIAKYELEDQSADLHPLFDTIIKELPAPKHTPEEPFQMLVADLD
                     YSDYLGQLAIGRVANGTVAKNEQLVCIGEGDKENALRASKVQVYEGFTMQEVEKIEPG
                     EIMILAGIENVRIGDTICTKERPKALKRITVDEPTISMFFYTNTSPFAGQEGKLVQSN
                     KIKERLHKETLSNVSLQFEETKDAERFLVKGRGELQMAILIETMRREGFELAVGRPEV
                     IYKEENGERLEPIEHVYVDCEEGFLGVVSEKLSQRKGRMINLVNHGSGRVQVQFNIPS
                     RGLIGYRNEFLTDTRGTGILNSYLSGYEPYRGDFPIRFTGSIVADRQGKGVPYALFNL
                     EPRGILFIIAGERVYEGMIIGEHNRDSDLNVNPCKEKKLTNHRASGKDENVVLTPIIP
                     MTLERAIEF"
BASE COUNT         1277 a          844 c         1028 g         1033 t
ORIGIN      
        1 tcgtcgcgaa cgcgaatcac aaatactaaa attatctaaa tccggaaatt ccaaagagga
       61 aattctggag cagctttacg aaggactaga cccacggctt caaccgttgg cgatgcaaaa
      121 tattgaatca catttggaaa agctgaacaa agaagaataa ataacataat gaaatcaaat
      181 aaaaaagcga ttctccgtat gctggaaaag caaggagatt cattgtccgt aaaacaaatc
      241 cgcggttcac taggactgga aaaagcagcg accgcaaaac tgaaagcaga attactgaaa
      301 ctgataaaag ccggaaaaat tgccaagcat ggcacccgtt accaaaaatc tcaggaaata
      361 actgaaagaa ctggttcaaa agcaaaaatt aaaggaaata gagaaaaagt taaaaatgaa
      421 ttaatctgga aacaaacacc tgaaaggcgg caaagaagaa gttcaggaaa aacagaaact
      481 ggctatttca cccgcaatcc aaagggtttc ggttttgttg caattggagg aggacgccct
      541 gacgtgttta tcggtgagca cgagcagggc tttgccatgg agggtgacct ggttgaaatt
      601 gaggtcttcc gtaaacgggg ttttcgcagc aaacaaaagg gtgaaattat caaagttctt
      661 gagcgtgcat caagagaaat actggcacgt ttaaaaagaa cgaagcgtga aaccgttgca
      721 gtaccggtac accgtaattc agggttgccg tttctccgta ttgctccgaa agatgatctt
      781 ccggaactgg aatccggaac gttggttgaa gttgaattac tggaagaaaa aaaacaaacc
      841 aaaaaaacat ttcctgctcc atgcgggcgt gtcttgcgtg cactggaaaa tacgagcgac
      901 cacgaactgg gttttcagct gattctcaat gaaaacctga ttcgtaccga atatcctgca
      961 gaagctctgg cacatgtaaa taatttttca cagcaggttc gttttgatgc atcttccgga
     1021 cgcagggatt tacgagattt ggattttgtg acaattgatg gaaaatccgc ccgtgatttt
     1081 gacgatgcag tctgcgtgat tcctgacaaa gactcttccg gaggatttcg tctttttgtg
     1141 gcaattgcag atgtggcgca ttacgttcaa ccggaagatc ctgtggacca ggaagcactt
     1201 ctgcggggaa caagtgtcta tttcccaacg catgcggttc caatgcttcc ggaaggactt
     1261 tccaacaatt tgtgcagttt gcgaccgcaa gttaaccggc tgacacttac ttgtgaaatg
     1321 cggattactg ccgaaggaga agtagtcggt tattcgcttt cagaaagcgt catccgtagc
     1381 cgtgcccgtc tgatttatga ggacgtggcc gacttacttg aaggaagaac ttcttccatt
     1441 cgcgatccgg aactgcagac gaacatccgg aaaatgcaca agctggcaaa gattcttgaa
     1501 cgcaaacgga ttcagcgtgg agccgtgcag tttagttttg ctgaagaagt ctttgagttt
     1561 gatgcagatc agcaaatgac cggagtagga cgaatatatc aatcgagtgc aatgaagctg
     1621 attgaacaat ttatgctgga agcaaacgaa acggtggccc gtcactgtgt gaaaaacaag
     1681 atgccggctc tttaccgggt gcacgaacgt ccggacatgc gaaaattgca gaaactgcag
     1741 caaacgtttt ttcgctttgg tgtaagaacg cctctggcaa aattggcaga tccacggcag
     1801 ttcaatgcag tcattgagca aattcaggaa ctgccgcatt tcgatcagct gcaggttctt
     1861 ttgctgcgta cgatggcttt ggcagtttat cagacaacca acaaaggcca ttttggactt
     1921 gctgcggaat attatgcgca cttcacgtcg ccaatacgca gatacccgga cttggtggtg
     1981 caccgtgcta tcaaggatat gctgcaccag gatcaaggtt taaggactgg aaaaagaaca
     2041 cttccacagg taaacagtga aatggcagaa cagtgttcgc agcaggaacg gcgggcagaa
     2101 aaagccgagc gccagagtat tgatttgatg aaagtggatt ttctggcgcc tcatgcagga
     2161 caaacttttc aggcagtagt gatctctgtt gacagccagg gttttcgggt gaaccttgaa
     2221 ccgcatggtc tcgaatggtt tttgccgctg gattcaatgc atgacgattc ttatattttt
     2281 gacgaagaac gactcagttt acagggacgt cgcaaaaacc gaacactaca ggcaggacag
     2341 cgcctggaaa taagactgct ccgtgcagat ccaattcacc gtatcctgga gtttgatgtg
     2401 gaacgctggc taagtaacaa atactaaaca aaaaacacat tgaaattatt aaaaaatgga
     2461 aaacacaatc tcaaataatc gtatccgcaa cttagctatc attgcccatg ttgatcatgg
     2521 aaaaaccaca ctcgttgatc aattgtttcg ccaaagcggc ctctttcgtg aaaatcagtc
     2581 tgtggacgag cgtatcatgg acagtatgga tttggagcgt gaaaggggaa ttaccattgc
     2641 tgcgaaaaac tgctcagtga cctggaatga aactaaaatc aatatccttg atactccggg
     2701 acacgcggat tttggtggag aggtggagcg tgccttgatg atggttgatg gtgtaattct
     2761 gcttgttgat gcctcagaag gtccattgcc acaaacccgg tttgtgttga aaaaagctct
     2821 tgaaggtcag aaaaaagtgt tggtggtggt caataaaatt gatcgtcagg atgcacgtgt
     2881 tgctgaagtt cttgatgaaa tatatgattt gttcatagat cttgatgcaa acgaggaaca
     2941 attggaattc cctgtcttgt atgcaatcgg caaggatgga attgccaaat atgaattgga
     3001 agatcagtct gctgatctgc atccgttatt tgacacaatt attaaggaac tgcctgctcc
     3061 aaaacatact ccggaggaac cttttcagat gctggttgca gatctggatt actctgatta
     3121 tctgggacaa ttggcaatag gtcgtgttgc aaatggtact gtagccaaaa acgaacagtt
     3181 ggtctgtatt ggtgaaggag acaaggaaaa tgccttgcgg gccagcaaag tccaggttta
     3241 tgaaggcttt accatgcagg aagtggagaa gattgagccg ggagaaataa tgatcttggc
     3301 aggcatcgaa aatgtccgga ttggcgacac catttgcacc aaagaacggc ccaaagcatt
     3361 gaaacggatt actgttgatg agcctactat ctccatgttt ttctacacca acacctcacc
     3421 ttttgctggt caggaaggaa aactggttca gtcaaacaaa attaaagaac ggcttcacaa
     3481 agaaaccctc tctaatgttt cgctgcagtt tgaagaaaca aaagatgcag aacgctttct
     3541 ggtcaaaggc cggggtgaac tgcaaatggc tattctcatt gaaaccatga ggcgggaagg
     3601 attcgaactt gctgtcggac gtcctgaagt gatttacaag gaagaaaacg gagaacgtct
     3661 ggagccgatc gaacatgttt atgtggattg cgaagaaggt tttctgggtg ttgtttcaga
     3721 aaaattatca cagcgaaaag ggagaatgat caaccttgtc aatcacggat caggcagggt
     3781 acaagtccag ttcaacatac catcacgtgg attaatcggt taccgcaacg agtttttgac
     3841 agatacccgg ggcaccggaa ttttgaactc ttatctttcc ggatatgagc cgtatcgcgg
     3901 cgatttcccg atccgcttta ctggatcaat tgtggccgat cgccagggta aaggtgtacc
     3961 gtatgcactt ttcaatctgg aacccagagg tattttattc ataatcgcag gagaacgagt
     4021 ctatgagggc atgataattg gtgagcataa tcgtgactcg gatctcaacg taaatccctg
     4081 taaagaaaag aaactcacca atcaccgtgc ttcaggaaaa gatgaaaacg ttgtattgac
     4141 tccaattata ccgatgacct tggagcgggc tattgaattc at
//
LOCUS       DTVD01000275            4130 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7794_length_4130_cov_313.284172, whole genome
            shotgun sequence.
ACCESSION   DTVD01000275 DTVD01000000
VERSION     DTVD01000275.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_4130_cov_313.284172"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(65..1408)
                     /locus_tag="EYO68_10145"
     CDS             complement(65..1408)
                     /locus_tag="EYO68_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIC08199.1"
                     /translation="MNKRVAVIGAGPSGLAQLRSFQSAAEKGAEVPEIVCFERQEDWG
                     GLWNYTWRTGLDEYAEPVHSSMYRYLWSNGPKECLEFADYSFEEHFGRTIASYPPREV
                     LFDYIIGRVEKAGVRKQIRFRTTIREVYYSVKSGRFTLTAHNLVDDTVYSEEFDNVVV
                     ASGHFTTPNVPSFDGIETFNGRVLHAHDFRDALEFKGKNLLLIGTSYSAEDIGSQCYK
                     YGAKSITCSYRTAPMGFHWPDNWEEVPLLKKVDKNTCTFKDGSSKEIDAIIMCTGYLH
                     YFPFLPDSLRLKTDNRLWPLNLYKGVFWEDNPKLMYLGMQDQFYTFNMFDAQAWYTRD
                     YMLGRINLPSKEEMRSHSQAWREREEKLENDEQMIWFQGDYVKELISETDYPSFDVEG
                     VNKTFMEWEHQKHEDIMDFRNHAYRSLITGNMQPVHHTPWMEAMDDSMESYLENK"
     gene            complement(1841..3901)
                     /locus_tag="EYO68_10150"
     CDS             complement(1841..3901)
                     /locus_tag="EYO68_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIC08200.1"
                     /translation="MNQLTNHPKLFQFVGLLVIFALLCLFINPSENNIFWRFPSLIAG
                     LPYLINDSVEYLMFDWWPIQVYDPEIEEFEEKPLLQQVTRAISASILFLIGLIREIIM
                     GGVKTIVTFTSWDFVSENKWARWPALPWTVVASGAILLGYKLQGKGLAMLAGFSTIYI
                     AVFGQWEPSMQTLSFVLFAAPVSFILGLALGIWAYKNRKVELTLNPFLNVMQIVPQFS
                     YLVPVMVFFGIGDHAGAIATIIFATPPMVRLTVLGLKRVSPEVLEAGMMSGCSNFQLM
                     YKVLIPTARRDILIGVNQVIMQCLAMAVIASFIGAKGLGFNLLLSLNGLRIGQAIEQG
                     ICIVLIAVVLDRMSLAWANKQTDYFADLTFFKRHKYSLMFLVIVVAGIVLAYIGTFFF
                     REGFNYLYIVPHNKGISIDDTLQNGVDWIWDTFFFTLKGFNVFLIQQILMPMKAAYLS
                     MPVVATLALTMGVGYIIGGIRSAAIVGGFILFIALTEWWDRALITMYLMSFAVIVSSI
                     IGVTVGTLCAQNSYTAKFILLVCDTFQTFPSFIYLIPVIMLFGVTDTSVLIAVIVYAT
                     VPATRYTVVGLRSVPPDLHDAGSMSGVNRLQRWIKIELPMSFPHMALGVNQTVVFALS
                     MIIIGAMIGTDDLGQFILKALSDKNGVGNGLTLGLCVAFIGLAADQVIRTWANERKQI
                     LDIS"
     gene            complement(3912..>4130)
                     /locus_tag="EYO68_10155"
     CDS             complement(3912..>4130)
                     /locus_tag="EYO68_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08201.1"
                     /translation="IESVMDPIDSSEELSDLQVSKDAIIETVAEVVLNQQKPVAVTDA
                     DNNVVGVVHSSKVIHVLFGSRAETENND"
BASE COUNT         1229 a          985 c          818 g         1098 t
ORIGIN      
        1 ttccggtact ctccaggagg aatactgaac gttagagagt ccctgcacag gctgtttttc
       61 actcttactt gttttccagg tatgattcca tggaatcatc catagcttcc atccagggag
      121 tatgatggac tggctgcata ttcccggtaa tcaaggagcg gtaggcatgg ttccggaaat
      181 ccataatgtc ctcatgcttt tggtgttccc attccatgaa ggtcttgttt actccctcca
      241 catcaaaact cggatagtcc gtttcggaga ttaactcttt cacataatct ccctggaacc
      301 agatcatctg ttcatcgttc tcaagttttt cttcccgttc tctccaggcc tgactgtggc
      361 ttctcatctc ctcttttgaa ggcaaattga tgcgtcccag catgtaatct ctggtgtacc
      421 aggcctgtgc gtcaaacatg ttaaaagtgt agaactgatc ctgcattcct aggtacatca
      481 gttttggatt gtcttcccaa aatacacctt tatagaggtt caaaggccag aggcggttat
      541 ccgttttcaa gcgcagacta tccggaagaa aagggaagta atgcagatag cccgtacaca
      601 taatgatcgc gtcaatctcc ttacttgagc catccttaaa ggtacatgta tttttatcaa
      661 ccttcttcag cagagggact tcttcccagt tatccggcca gtgaaatccc atcggagcgg
      721 ttctataact gcaggtaatg gactttgcac catatttata acattgggaa ccgatgtctt
      781 ctgcagaata actggtgccg atgagcaaga gattttttcc cttaaattca agcgcatccc
      841 gaaagtcatg tgcgtgtagc acacgaccgt tgaaagtttc tattccatca aaacttggca
      901 cattcggcgt agtaaaatgc cctgaagcca ccaccacatt atcaaattcc tcagagtaaa
      961 ccgtgtcgtc cactagatta tgtgcagtta aggtaaagcg gcctgacttt actgaataat
     1021 aaacctcacg gattgtggta cgaaaacgga tttgcttcct gacaccggct ttttcaacac
     1081 gccctatgat gtagtcgaaa agaacttcac gtggaggata tgaagcaata gtgcgtccaa
     1141 aatgttcttc aaaggagtaa tccgcaaatt ccaaacactc ctttgggccg tttgaccaca
     1201 gatagcggta catgctggaa tgaaccggtt ctgcatattc atcaagtccg gtacgccagg
     1261 tataattcca cagtcccccc cagtcttcct gccgttcaaa acatacaatt tccggaactt
     1321 cagcgccttt ttctgcagcc gattgaaaag accttaattg tgctaaaccg ctgggtcccg
     1381 caccaataac tgcaactcgc ttattcatga caatttctgc ttaggttaat gatcgaaata
     1441 tttacaattc taactgaatg aatttaattt actaccacac cgcagagcag cgttgtatgc
     1501 tgtgacactt ttgtgaattg tgcagcaatt ctctaatacg aacctgctgt tccccggcca
     1561 attatattta ttgttttgtc tttcaagtac atctccggat tgacaacctt tatcacaact
     1621 cccaaagcta gagaattcca tagggcattc ctgctactcc tctctcccag atagaattac
     1681 attgaatcgt ccactgcttc cagccacgga aaagattaga gtagtgggcg tatttaaacc
     1741 gaataaaaga cactagctaa aatagatgta attttttgtt acaattgctg ttgggttgga
     1801 tgtcaattaa tggcttcaac cgaagaaaag aagggtagtt ttagctaata tctaaaattt
     1861 gttttctttc gtttgcccaa gttcgtatca cttgatcagc ggccagacca atgaaagcca
     1921 cacacaggcc cagtgttaat ccattgccta cgccattttt atcggataaa gctttcaaaa
     1981 taaattgtcc aagatcatcg gtaccaatca tcgcgccaat gatgatcatg gataacgcaa
     2041 acacgacggt ttgattaacg cccaacgcca tgtgtggaaa agacatgggc aattcaattt
     2101 tgatccatct ttgcagtcga ttgacacctg acattgagcc cgcatcatgg agatctggtg
     2161 gcacactgcg cagtcctacc acggtataac gggtagcagg aaccgtggca tagacaataa
     2221 ctgcgattaa tacggaggta tcggttacgc caaaaagcat aatcacggga atcaaatata
     2281 taaaggatgg aaaagtttga aacgtatcac aaaccaataa aatgaatttt gcggtatacg
     2341 aattttgcgc acataaagtt cccaccgtaa cgccaataat ggatgacaca atgactgcaa
     2401 agctcattaa gtacatcgta ataagggctc tatcccacca ttctgtcaaa gcgataaaca
     2461 aaataaatcc gccaacaatt gctgccgatc gtattccgcc gatgatgtaa ccaacgccca
     2521 tggttaaagc aagcgttgca acaaccggca tactgagata cgcagctttc atcggcatca
     2581 ggatctgctg aatcaaaaat acattaaacc cttttaatgt gaagaagaag gtatcccata
     2641 tccaatcaac gccattttgc aaagtatcgt caatcgagat gcctttgttg tgcggcacaa
     2701 tatacaaata gttaaaacct tctcgaaaaa agaacgtgcc aatgtaggcg agtacaatgc
     2761 ctgctacaac aatcacgagg aacatcaatg aatatttgtg gcgcttaaaa aaagtaagat
     2821 cagcaaaata atccgtttgt ttgtttgccc aagcaagaga cattcgatct aaaaccaccg
     2881 cgatcagcac aatacaaata ccttgttcta ttgcctgccc aattctgagc ccattcagtg
     2941 aaagcaataa attgaaaccc aaacccttag cgccaataaa agaggcaatc accgccatgg
     3001 ccaagcactg catgatgacc tggttgacgc caatcagaat atcccgtctt gcagttggaa
     3061 ttaatacttt atacatgagt tgaaaattac tgcagccgct catcatgcct gcttccaaaa
     3121 cttccggcga cacccttttc agccccaaca ctgtcagacg caccatcggg ggggtggcaa
     3181 aaataatggt cgcaatggca cccgcatgat cgccaattcc aaaaaatacc atcaccggca
     3241 ccaaatagga aaattgcggc acgatctgca tgacgttaag gaaaggattc agtgttagtt
     3301 cgactttccg atttttgtac gcccagatac ccagagctaa gcctaaaata aaagaaacgg
     3361 gcgctgcgaa cagtacgaat gagagcgttt gcatggacgg ctcccattga ccgaaaacgg
     3421 caatgtatat ggtcgaaaag cctgccagca tcgcaaggcc tttgccctgg agcttatagc
     3481 ctaaaagaat tgcgccgctg gcaacgaccg tccagggcaa tgcgggccaa cgggcccact
     3541 tgttttcact aacaaaatcc cagctcgtaa aagtaacgat cgttttaacc ccgcccataa
     3601 taatttcgcg aattaaccca attaaaaaaa ggatgctcgc cgagatggct ctcgtcactt
     3661 gttgtagtaa cggtttttcc tcaaattctt caatctcggg gtcatagacc tgaatcggcc
     3721 accagtcgaa catcaagtac tcgaccgagt cattaatcag gtatggaaga cctgcaatca
     3781 gtgaaggaaa acgccaaaaa atattattct ctgacgggtt gataaagagg cagagtaagg
     3841 cgaaaataac caataaacca acaaattgaa ataatttggg atggtttgtt aactgattca
     3901 tagtcgtaga attaatcgtt attttccgtt tcagccctac tgccaaacaa cacatgaatc
     3961 actttggaag agtgtaccac tccaaccaca ttgttatccg catctgttac cgcaactggt
     4021 ttttgttgat tcagcactac ttccgcaact gtttcgatta ttgcgtcttt agagacttgt
     4081 agatcgctta gctcttctga gctatcaatc ggatccatca cagattcaat
//
LOCUS       DTVD01000276            4098 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7887_length_4098_cov_383.782093, whole genome
            shotgun sequence.
ACCESSION   DTVD01000276 DTVD01000000
VERSION     DTVD01000276.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7887_length_4098_cov_383.782093"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="msrA"
                     /locus_tag="EYO68_10160"
     CDS             complement(<1..965)
                     /gene="msrA"
                     /locus_tag="EYO68_10160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIC08202.1"
                     /translation="MSKLFFIILFIFGLNCLTSPATFAGKDLEKAIFAGGCFWCMESD
                     FEKISGIKDVISGYIGGTTKNPSYQNYGRAGHIEAVLIPFDPSVISYAELLEIFWIKI
                     DPTDKNGQFCDRGHEYSTAVFYLSTEQERLAEESKSLLEKSGILKESVKTPIIMAGEF
                     YPAEDYHQNYYIKSKLKYLYYRFRCGRDQRLKELWGEKINKVKAFNKTSNIQKPDLEE
                     LKKILTPLQFEVTQNDATEPPFRNKYWNNKREGIYVDVVSGEPLFSSTDKYKSGTGWP
                     SFTRPIEGASLIQKEDNGWFSVRTELRSKKADSHLGHLFNDGPKP"
     gene            complement(984..2033)
                     /locus_tag="EYO68_10165"
     CDS             complement(984..2033)
                     /locus_tag="EYO68_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIC08203.1"
                     /translation="MVKTKFNKTFFGTELPIIQAPMAGVQDSALTIAVSNAGGLGSLP
                     CAMLRPDALRAELKAIKSQTSKPFNLNFFCHTAPEPDLEREAKWRKILQPYFKEYGLK
                     KSDIPTGSGRIPFNQESADLLAEYKPPVISFHFGLPADSLLAQIKNLGAYVLASATNL
                     EEAKWLEANGTDAIIAQGLEAGGHRGIFLNNDLSTQVATFELLKQIVGISKLPVIAAG
                     GISTANDVAKALSLGASAVQIGTAYLLCTETKTSQIQRAAIKSKKTRKTVITNIFSGR
                     PARGIENRSIREIGPINADTPEFPLAAAAISALRTKAEAVGVDDFTPLWCGENISGCS
                     EIPAAELTRLLVTEL"
     gene            complement(2138..2380)
                     /locus_tag="EYO68_10170"
     CDS             complement(2138..2380)
                     /locus_tag="EYO68_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08204.1"
                     /translation="MRHLWVFVLTVFQDNYVRTGTLLGLLAGFWSTEIIGGEPTTNAL
                     IYGFIGWGVGVYLGHNRSENSHYPKKKKFKVFQGGG"
     gene            3100..3867
                     /locus_tag="EYO68_10175"
     CDS             3100..3867
                     /locus_tag="EYO68_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC08205.1"
                     /translation="MEIPPLFNLTEKVALVSGASRGIGAAIAQILAEFGANVILTSRR
                     AENLKEVETAILKSGNQASSMVCHNGDLAQIESLFQNIKKIHGRLDILINNAATNPFY
                     GSVLDADEAAWDKTLDVNLKGPYFMCQHAAKLMRKNGGGSIVNVSSINGRIPMHQQSI
                     YSITKAGLISMTQAFAKELGGENIRVNALLPGLTDTKFASAVTQNEELMKTVLPQIPL
                     GRIAQPSEMSGMVLFLVSDAASYVTGSTFTVDGGVLA"
BASE COUNT         1205 a          854 c          831 g         1208 t
ORIGIN      
        1 tccggttttg ggccgtcatt aaacaagtgt cccagatgag aatctgcttt cttgcttctg
       61 agttcagtcc ttacagagaa ccagccatta tcttcttttt ggattaaact tgctccttca
      121 ataggtcttg taaaactggg ccagccggtg ccggatttgt atttatctgt tgaactgaac
      181 agaggttcgc ctgaaactac atcaacataa ataccttctc tcttgttatt ccagtattta
      241 tttctgaaag gcggttctgt agcatcgttt tgcgtaactt caaactgtaa aggagtcagg
      301 atctttttga gttcttccaa atccggtttt tgaatattgg atgttttatt gaatgcttta
      361 accttgttta ttttttcgcc ccataattct ttaagccgct gatctcgtcc gcatctaaaa
      421 cggtaataaa gatatttcag tttgctcttt atgtagtaat tctgatggta atcctctgca
      481 ggataaaatt caccggccat aataatcggc gttttaacgg actctttcaa aataccggat
      541 ttttcaagca gagattttga ttcttccgcc aatcgttctt gttctgtaga aagataaaaa
      601 actgcagttg aatactcatg acctcggtca caaaattgtc catttttatc agtaggatca
      661 atcttgatcc aaaatatttc cagcagttct gcataagaaa taacagacgg gtcaaaagga
      721 atcaacactg cttcaatatg accagctctt ccataatttt gatagcttgg atttttagta
      781 gttccaccga tataacctga aatgacatct tttattccgg aaatcttttc aaaatccgat
      841 tccatacacc agaaacagcc tcctgcaaag atggcttttt caagatcttt tcctgcaaaa
      901 gttgcgggtg aagtcaggca gttcagtcca aaaataaata agataataaa aaataattta
      961 ctcatactgc tcctataaat actttaaagt tcagttacca gcaaccgtgt taattctgca
     1021 gcaggaattt cactgcagcc gctgatattt tctccacacc aaagtggtgt aaaatcgtca
     1081 actccaactg cttcagcttt tgtacgtaaa gcagaaattg cggcagcagc aagcggaaat
     1141 tccggtgtat cggcattgat tggaccaatt tctcggatgc tccggttttc gattccacgg
     1201 gccggtctgc cgctaaaaat atttgtgatg acagtcttgc gggttttttt actttttata
     1261 gctgcccgtt gaatttgact ggtttttgtt tcagtacaga gcagatatgc tgtgccgatc
     1321 tggactgcag aggcgccaag tgaaagtgct ttagcaacat catttgcggt tgagattcca
     1381 ccggcggcaa ttacaggcaa tttggaaatc ccaacaattt gttttaaaag ctcaaacgtt
     1441 gcaacttgag ttgagagatc attgttcaaa aaaatgccgc gatgacctcc tgcttcaagc
     1501 ccctgggcaa taatcgcgtc cgtgccattt gcttccaacc attttgcttc ttcaagattg
     1561 gttgcagagg ccaaaacata tgcacctaga tttttaatct gtgccagcaa actgtctgca
     1621 ggtaaaccaa aatggaagct gattaccgga ggtttatatt ctgccagcag atctgcactt
     1681 tcctgattga atggaatacg tccggatcct gttggaatat cactcttttt caaaccatat
     1741 tctttgaaat agggctgcag aattttgcgc cattttgctt cgcgctcaag atctggttcg
     1801 ggagcagtat gacaaaaaaa atttaggttg aagggcttgc tggtttggga tttgatggcc
     1861 ttcaattctg cacgaagtgc atccggtcga agcatcgcac agggcaggct gcccaaacca
     1921 ccggcattgg aaacagcaat tgttaaagca ctgtcttgaa ctccagccat tggagcctga
     1981 attatgggaa gttctgtacc gaaaaaagtt ttgttgaact tggttttgac catgacagaa
     2041 aatttcagca aacaaagaga aaaagcatcg cagtctgtgc gaaccgaaaa attaaagaga
     2101 aacagctctt aaagtgagtt ctcgcaaacc acaaaaacta acctccaccc tgaaagactt
     2161 tgaatttctt tttttttggg taatgtgaat tttcggaacg attatggccc agataaactc
     2221 caacgcccca accgatgaag ccgtatatta gagcatttgt agtcggctcc ccgcctataa
     2281 tttctgtgct ccaaaatcct gccagcaggc ccagcaaagt gcctgttctg acatagttat
     2341 cctgaaaaac agtcaacaca aaaacccaca aatgtcgcat cattttgtta aaaatagaat
     2401 tatcagcaca gctgacagta actgcagatc gttattttcg tcggttgtct aagctaaaat
     2461 actaaagacc ttatggtttg agatagtgcc aggaacattc ttgaagaaaa atatataaca
     2521 agaacaccac ctataaacaa atcaccctct ccctgtatgg gatgaaaaaa cattgtaaaa
     2581 tttgacggag gttccagttt ttcatccaaa ggtctcttct ttcgaaagac aatcattgcc
     2641 acgagtgata gaatagttaa agcgatccag tttcccattt tatttaatac tgaagaagaa
     2701 ttatgcagtt ttgatcatat cagtcaaaac gtctccttgt atagcttata cgagatgaac
     2761 taatgtgaca acgcaaaatc tatgcaatca taatcaaaaa ctaatgcttt tcgtaactgt
     2821 ctttatatga atgatgccgt agtaaaaagt cctgtaactg tgtcattacg agcgaagcga
     2881 aaaatctagt ataagcaacc gcactaatat cattaagatt cctccctacg gtcaaaatga
     2941 cagattagat ttcagacttt ttttgagacc gtcaaaaata actttctggt aataatactt
     3001 aaatctctcg ctttcgagga ctactgtctc tttcaaaacc gcattgcgtc ttcagttgac
     3061 aaacgataat atggaataca cataaatgaa ggagcatgta tggaaattcc accattattt
     3121 aacttgactg aaaaagttgc cttagtttcc ggtgcaagcc ggggaattgg ggcagccata
     3181 gcacaaattc tggctgagtt tggtgccaat gtcattctga ccagccgcag ggcagaaaat
     3241 ttaaaagaag ttgaaacagc aattctaaag tctggcaatc aggcttccag catggtttgc
     3301 cacaatggtg atcttgcaca gatagaatca ctttttcaaa acattaagaa aatccacggc
     3361 agactggaca ttttgatcaa caatgcggca accaatcctt tctacggttc ggtcctggat
     3421 gcagatgaag cggcatggga taaaacactg gatgtgaacc tgaagggacc atatttcatg
     3481 tgccagcatg cggcaaaact aatgcggaaa aacggaggtg gatcaatcgt aaatgtttct
     3541 tcgataaacg gtcgtattcc gatgcatcag caaagcattt attcaattac aaaagcaggc
     3601 ctgatttcca tgacacaagc ctttgccaaa gaacttgggg gcgaaaatat tcgcgtgaat
     3661 gcgctgcttc ccggtctcac tgacactaaa tttgcctctg cagtcacaca aaatgaagag
     3721 ctgatgaaaa ctgttttacc gcaaattcca ctgggcagaa ttgcccagcc gagtgagatg
     3781 tccggaatgg tgctttttct ggtttctgac gcagcttcat acgttacagg cagcacattc
     3841 acagtggacg gtggtgtatt ggcttagaca atagtgctgt actttcaggg acaggaaaag
     3901 cttcgctcga atgacagttt tttgttccag ctggaatgac agttttgtgg ttttctcgaa
     3961 ctgggttctt gtttcgtgat ttcagttttc ttgtagtgtc aaagacaagc tttgcgggaa
     4021 atcagtaatt caggaataca tagtctactg tcttggtagg gagttgtcat tcccgcgcaa
     4081 gcgggaatcc aggggtgt
//
LOCUS       DTVD01000277            4074 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_7958_length_4074_cov_303.534959, whole genome
            shotgun sequence.
ACCESSION   DTVD01000277 DTVD01000000
VERSION     DTVD01000277.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_4074_cov_303.534959"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            223..558
                     /locus_tag="EYO68_10180"
     CDS             223..558
                     /locus_tag="EYO68_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08206.1"
                     /translation="MIFFWYSVSVYISVSAQNLTPEILACSESAQARYACFKKGRKGE
                     FERAKEMESIRRSARDTKEEFLLVSIMHSKRQCEKLSSGNNAANKVFTFPKYFSKMRK
                     ILPYLRLYG"
     gene            complement(755..1462)
                     /locus_tag="EYO68_10185"
     CDS             complement(755..1462)
                     /locus_tag="EYO68_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08207.1"
                     /translation="MTFEERIDWFSARNLIMLFLLKDRFLNPLVPVQLQKLKSSGLLD
                     NKYLLKVMEEHFPEYDAELPRGMYFPVPISRSLSDGEDFSTKLAGQFFYDYIHVDDHK
                     KWSLRDKYITGKVLSLFESNLFYEKETNRYYVEYWSDSRWDKCYLECAITPMLGLSVE
                     SIPDGLKLELNNHKTDLIDLHSFRIDTKERCFALSLNHGEVQLGDTPRFWLLNQLDET
                     GTQLVLNKQLFPLNISS"
     gene            complement(1529..2854)
                     /locus_tag="EYO68_10190"
     CDS             complement(1529..2854)
                     /locus_tag="EYO68_10190"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08239.9,HMM:PF13354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08208.1"
                     /translation="MLFCLLPEVCLPFFLLLFSLSPLFAKRDSPFSNQFPPHSLQGFY
                     SAKQSKDKPTFSVIKGSFAVVSATSLNVRSGPSTKNPVLKVILKETHILPLSSPKHEW
                     LKIRIPKGIKGWVAKKHVKIYMPKPLSVFKVKPASMPFTSLLEASISRYMEEMYIQKR
                     LKPVDKLFIVVEDLTTESYVVSIRPRQSVKSASTIKVPILHAFMIQRFRGNIKEPSKY
                     ERQIEEMIRFSSNPSTNTIIKLLGGPKIIQDLLNETKIYKELKLAEAIPEDGRTYQNK
                     ISAADLNQIFVNIWSNRVIGSEFNLENNMTASKKMLHLLKLPGHSWLIDRIKAGTCFS
                     SNKSVKIWDKTGFVKGVNGNGGIVEIDTPHGRKAYSIVMFMERDKYHTIIGDATKWSE
                     RMAMHMRRISEMTYAFFSNRYESYNKCGRSLLNRFAKLALASHLLHASL"
BASE COUNT         1267 a          718 c          739 g         1350 t
ORIGIN      
        1 accaagtttt ttgtatgata tactatatga tatttggctt ctggttgagg tttttgtaca
       61 tttattttac ggacattatt atttttgcta tttccctcaa cagttgtgtt ttcagtttga
      121 gcgaacccaa aatttatgta atttacgaag acccagccaa aaaaaacgac tgcaaaacta
      181 cgtcggacta tacatcctag tctaaggtcg tttttagcag caatgatttt cttttggtat
      241 tctgtctcag tatacatttc tgtctctgct caaaatttaa ctccggaaat attagcatgt
      301 tccgaatctg cacaagctcg ttacgcctgt tttaaaaaag ggaggaaagg tgaattcgaa
      361 cgtgctaaag aaatggagtc tattcgacgt tccgctcgtg atacaaaaga agaatttctt
      421 ttagtatcaa tcatgcactc caaaagacaa tgcgaaaaat tatcttctgg aaataatgca
      481 gcaaataaag tattcacgtt cccgaaatat ttctcaaaaa tgagaaaaat actcccttac
      541 ttacggttat atggttgaac tgaaaccata agcatcttag attatgagcg gtttaataaa
      601 gaagctctat taatacttct taaaaatttg tgccaattat aacatgatag ttaaatattt
      661 tcaatgaata ttgctgaatg gcaacaatat gaagtagatg tctcaggtag gtaaggaact
      721 taaatatgga ggaaatgcct attattattt caattcatga tgaaatattc aaagggaaaa
      781 gttgcttgtt cagaacgagt tgagtacctg tttcatccaa ttgatttagc aaccagaatc
      841 tgggagtgtc tccaagctgt acttcgccat ggtttagtga gagggcaaaa cacctttctt
      901 ttgtatcaat acggaaacta tggagatcaa ttaaatctgt tttatgatta ttgagttcca
      961 actttaatcc atctggaata ctctctactg ataatcccaa cattggagtt attgcacatt
     1021 caaggtagca tttatcccat cgactatcac tccagtattc aacgtaatag cggttggttt
     1081 ccttttcata aaacaggttt gactcaaaaa gcgataaaac ctttccagtg atatacttat
     1141 ctctcagact ccatttttta tggtcatcaa catgaatgta atcataaaaa aattgccccg
     1201 caagtttagt tgaaaaatct tctccatccg aaagtgaacg tgaaattgga actggaaaat
     1261 acattcctct cggcaactcg gcatcatact caggaaaatg ttcttccatt actttcaata
     1321 aatacttgtt gtcaagcagt ccggaagatt taagtttttg taattgtaca ggaacaagcg
     1381 ggttcaaaaa acgatctttc aatagaaaca gcatgattag gtttcgtgca gaaaaccagt
     1441 ctatacgttc ctcgaaagtc atgaatattt tgttaataat aatgttattg ctcccctgtt
     1501 tcagcaatca atatgctttt gagaaaattt agagagaggc atgaagaaga tgtgatgcaa
     1561 gggcaagttt ggcaaaacga tttaataatg aacgaccaca tttattgtag ctttcataac
     1621 ggttggaaaa aaatgcataa gtcatttcag agatccgacg catatgcatg gccatacgtt
     1681 ctgaccactt agtagcatct cctatgatag tgtgatattt gtcgcgctcc atgaacatta
     1741 caattgaata tgcttttctg ccatgaggcg tatctatttc aacaattcct ccatttccgt
     1801 ttacaccctt tacaaatccg gtcttgtccc aaatttttac agatttattt gacgaaaaac
     1861 aggttccagc tttaattctg tctatcaacc atgagtgtcc gggcaattta agtaaatgca
     1921 gcattttttt tgatgcagtc atgttatttt ccaggttgaa ttcagaacca attacacgat
     1981 tggaccaaat attaacaaaa atttgattta aatctgctgc agatatttta ttttgatagg
     2041 tccgtccatc ttcaggaata gcttccgcca acttcagttc tttgtatatt tttgtttcat
     2101 tcagtaaatc ctggattatt ttggggccgc ctaacaattt gataatggta tttgtacttg
     2161 ggttgctgct gaatcgaatc atttcctcaa tttgccgttc atacttcgaa ggttctttta
     2221 tgttcccgcg aaagcgctga atcataaaag catgaagtat tgggacctta atcgtacttg
     2281 ctgattttac actttgtctt ggacgaatag ataccacata agattcggtt gtcaggtctt
     2341 caacaacgat aaataatttg tctactggtt tcaatctttt ttgtatatac atttcttcca
     2401 tgtaccggct aatacttgct tccagcagag atgtgaaggg catagaggct ggttttactt
     2461 tgaaaacaga caaaggtttt ggcatataaa tctttacatg ctttttggct acccatcctt
     2521 ttatgccttt tgggatacgg attttgagcc attcatgttt tggtgaagac aggggcaaaa
     2581 tatgtgtttc cttaaggatc acctttagaa caggattctt agtacttgga ccggaacgaa
     2641 catttaatga tgtagcagaa actactgcaa agctgccttt aatgacagaa aatgtcggtt
     2701 tatctttcga ttgttttgct gagtaaaaac cctgcaatga gtgaggtgga aattggttgg
     2761 aaaatggaga atcccttttt gcgaaaagcg gacttagaga aaacaataat aaaaaaaacg
     2821 ggaggcagac ttcaggcagc aaacaaaaca acatagtgct gccatttgct cttattaaac
     2881 tgagtctatt tttcaaagta aaactcaaag aaaattagct gctagaaatg cggcagctcc
     2941 tgcagtgtga ttttccgatg agattcgaaa gggtggggtg ccttcaaatc attcaatggg
     3001 aaggctgaaa gaaaagagtt cccgagtcct ggaaaaggcg aattaggtat ttccgatttt
     3061 ataatgacag aaattaaccg tacttactct ttgcgaattc gactgatgtt agtcaaaaaa
     3121 acaattcctg gaataattat gaatccaatt accaaaacac tgaatacaat cgtccaactc
     3181 agtcccattt atgctccttt aaattaaagt atttgaaaaa tcctgataat aaactctatt
     3241 ctaataaaat taaaaagcaa actaaaatca acagtccatt gaatagactt agtgccaaac
     3301 tgtacctaaa tatcaggaaa atcttactta gtaaaaatct aaatatcagt cattattatt
     3361 tattttttac tgaaaataca aatgctcctc taaaatcagg gttgttttgt ttcagtctat
     3421 tgcgcaactt ctcagcttct ttttttgatt ttaacccgcc aatttgaact ttttgaaaaa
     3481 gtattttttg tgtagtacgt tgtattttaa aaaaaatctg attcttaaaa gcttgattta
     3541 agtatgttct tactctatta gccgtttcca gttctggaaa taaacctagg gaaacctgga
     3601 accctttttt tatcgattta cgataagcgt atcctgccag agatttgtcg gagttaattt
     3661 tctttacaat ttttgcagat tcttcttctc ccaaaacatt tgttgatata acttcggcta
     3721 taccggaact ttcactggaa gtccggacac gtgttttata gccaaggctt tgtaattgaa
     3781 ggctactttc atttacacat tcatgggaga tacaggaaat aacctgtatg taataactgt
     3841 atttatttgt tttggtccta agttttgact ttttaggttt aggtttgctg agttttgcaa
     3901 caggaggggg ttttggagga ggttctaaca ctgtttcagg cgattgagtt tcagaaggag
     3961 tggccaagta atatagaact gcaatcgtga tggagcccaa aatcaaaccg gatacgcagg
     4021 atagaattac cgcttttagc ggaaaacctt tggaggctgc tttccttttt gatt
//
LOCUS       DTVD01000278           15083 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_799_length_15083_cov_426.823396, whole genome
            shotgun sequence.
ACCESSION   DTVD01000278 DTVD01000000
VERSION     DTVD01000278.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15083)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15083
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_15083_cov_426.823396"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(56..457)
                     /locus_tag="EYO68_10195"
     CDS             complement(56..457)
                     /locus_tag="EYO68_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08209.1"
                     /translation="MASTFKLNIIRYRFMTFAIKTCSGARFFFFCLICLVSIVFLGEF
                     VENDGVLAQEKQTLKCPELSNPNSSEELKELRDCLQKIKFEIQSGRRMQKIQRSYEGS
                     LELVKQLNLVMDNKTSKQITEDSLNNNSADQ"
     gene            540..1058
                     /locus_tag="EYO68_10200"
     CDS             540..1058
                     /locus_tag="EYO68_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08210.1"
                     /translation="MLKLFIAAISVGLIYWVWLKKKQIFSNRNNKIDPFITTIEAIEI
                     QTFLDWNTSQYCLECDGIRYGKQFKQKKAPDLPHEAGCRCEATKLFYTSDDVFQGTSP
                     VLTHKSALGDLSTKDALLLKNILLKIRTSPGKGNFSDFLEQFEINNFSENIRSGAISL
                     VEHAFEAEQNKE"
     gene            1166..3550
                     /locus_tag="EYO68_10205"
     CDS             1166..3550
                     /locus_tag="EYO68_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIC08211.1"
                     /translation="MKRLAQFIKWSFRIVIITGIISGASLGVFLFELSKTLPQNLGDE
                     LHKRNDVLPTVLYDREGNQIEELFIQRRIVIPYEQFPPHLIQALVASEDARFFSHLGI
                     DLLRIPKAFLANLQAGRIVQGASTLTQQTARLFLLSREKQIIRKLREMLLAFRMEMQF
                     SKQDILSLYLNKVYLGNAEGVEAAAQGYFGKHASELNLAESALLVGILPAPSRYAPHV
                     NPDFALQRRNVVLQRMHQEGFISEQELQKTIHTPISLIRIHDSTTEATSYYVEHVRRY
                     LIKKYGSKVLYQGGLRVYLAMDLNYQTHAHEALRKGILELTKRQGFRGALNENSPDNS
                     SLTDNSTDENTLKIDSLFLGNIVAGVVKEVSKENVSVELGESYGILEWSNISTWKNGN
                     ILKDKTPIKISNPAEIFSVGDEVQVRLADYDSKNNFFRLQLYQEPLVNGALLAMNPKS
                     GEVLSMTGGYRYGESEFNRSIQALRQPGSSFKPIVYSAALDAGFTLSSALIDSPRAYA
                     TGAETAGDAEIWTPKNYGDKVMGKVSLRTALVKSLNLPTIGLCEELKPKLVIAYSRRF
                     GITAEMMENLTTCLGSLSVTLQEMISAYGVFANQGRLVKPIYILRVEDQDGNILESSV
                     SELKQVTSEETAFLVSNVLQDVVQRGTGRRARAIGRPSAGKTGTTNDSIDAWYIGYIP
                     QLLTGVYVGFDKPRRMGKSETGSRAAAPIWINFMKNAVANLPTEQFRQPPGITTVKIH
                     KSGRRAIPCDKAKDVYEEHYKSGTEPVLDLSQSGMCVKAKETEKTEKEESEPEL"
     gene            complement(3626..3901)
                     /locus_tag="EYO68_10210"
     CDS             complement(3626..3901)
                     /locus_tag="EYO68_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIC08212.1"
                     /translation="MTKADLINALAADTGLTKADADRFLKSFTATVQKTLQRGDSLTL
                     IGFGTFSVSHRAARVGRNPQNPSQVIQIPASKGAKFKAGSKLKKSLN"
     gene            4100..5377
                     /locus_tag="EYO68_10215"
     CDS             4100..5377
                     /locus_tag="EYO68_10215"
                     /inference="COORDINATES: protein motif:HMM:PF13103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08213.1"
                     /translation="MNFFQKLYKFVVFPGISAGIRTSARNLNGMEKFFIFHDQGTFSF
                     IFAASVLLHIVLVIITMAISELWVQELPPIRAKIEVRFAKIPSEPAPIEKPKPVLKKI
                     ENEWQPKLSNLVPKKPVLEKPVLKDTLKKSTLSKPEITQPEAPRLKMSEPQLAPSVPT
                     AKLNKISAPKKPLLLPVDSLEKSPLIPSLPKLSKDPVPLSPLRSKKSKLNPSQLVLPK
                     ISLEDITPKKINAPKIINMPKKLNKPKIKNSQKFSKPLNLPVRELPETMQSALQKVPS
                     IPQVQPSDKLGTNLREIFPDKIKFDEPLPDLGIPEQADETKLKEIPDTVNLQRKKLAQ
                     LAGEEYNLHIRTRIIPKLGSYSSELYVRIRLRIITTGKIIDYEIIKKSGFAAFDKAAE
                     LAVRNALLDPLPNALAENPPYIVTIRIIPQSKK"
     gene            5394..6755
                     /locus_tag="EYO68_10220"
     CDS             5394..6755
                     /locus_tag="EYO68_10220"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08214.1"
                     /translation="MHSVIYIFALRKFRIYFTIFFLCFSTLVQAQTYSSEEIVISGLG
                     FARFSVALVPEKSFTDHMDAKRWLRIIDRNLCWSGVFLVTDSRYRTCRTAGGNQVDMK
                     IMLKLKGTVTDNGDLVPKYLILTVADNDGAPLFPLELPIRKNRFREAELMDLINEMSV
                     QLTGLKGILGSTIAFTLKQPKRRKIIARINTHGKKLAAVSRNKYINLLPSWSPKGDAI
                     VYTTLSRRGTAIMFNDCLKVVGCKYRPVKLLSSNISAARISKNFLSVSGGTWFSNGHK
                     LIVTLSRKGNSDLYEFDRLKRKVIRLTTHRAIDTVPDLSPDNQHLIFVSDRSGHEQIY
                     YLKLGTKIPFQLTFGRGSSSDPVWSPDGTLIAFSKISSGHSQIHLMDPFTGEDNFLTR
                     GRFNSEQPFWSPDGRQIAFVSSSTGVDKLYIMFIDGTGRRRLTRTPRDFEEGGPSWTA
                     RKF"
     gene            6755..7063
                     /locus_tag="EYO68_10225"
     CDS             6755..7063
                     /locus_tag="EYO68_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIC08215.1"
                     /translation="MQFEDWQTAPDPKVIRKEKQKARELRKSQWWKNRRACNSCYYCE
                     SPTPAKKLTMDHVVPLARGGRSIKSNLVPCCKSCNNQKKNLLPIEWKEYLAIIGKKKE
                     "
     gene            7223..7627
                     /locus_tag="EYO68_10230"
     CDS             7223..7627
                     /locus_tag="EYO68_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08216.1"
                     /translation="MKSNKDVEQTNDELVNRQFFSLLTNKYQDQGKMKNKFNRQGNRF
                     GSREKSKAKGGANSSKGHPTSKEIELATQAYLSEGGKIDRVALNHGNAEKEVFRANLE
                     ADSRLGMGTLVDDSWDCWENKDYDSNPTGSGI"
     gene            8646..9248
                     /locus_tag="EYO68_10235"
     CDS             8646..9248
                     /locus_tag="EYO68_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgN family NAAT transporter"
                     /protein_id="HIC08217.1"
                     /translation="MDILSTGLTLLLIMDPLGNIPLFLSVLKTVDDESRKRKILIREL
                     CFALLVLLIFLFVGQYLLQWLNLRQEAVSIAGGIVLFLISLRMIFPTEKGIMGEMPAG
                     EPFFVPLAVPLLAGPSTLAMLILLARSQPDRIFEWLIAVLGAWVVTSLIMLSSTKLHK
                     LLGVRGLIAVERLMGMVLVAISVQMLLDGITTYLSVIPSL"
     gene            9490..10497
                     /locus_tag="EYO68_10240"
     CDS             9490..10497
                     /locus_tag="EYO68_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIC08218.1"
                     /translation="MPFKYLLIATFGALLFINYSGTAATKAADLSQKKAEFLPLASFL
                     YANRDPMRVGWYNTSSMPLEKDFKTDAKRPVGSFFVQLKPRKNEGEYQLALRVLRDGS
                     HQFLLIREFTNGNTLNHKRYLTIPFKLLIGAIQGEALRSLFPNDHVEFGGWRHQVTYV
                     WETPELLMKSFTRSGTQTILQKDFMQGEEYTIPWKNLRSDLELEPLAVRDPLFIRKDK
                     SGLRYAFYRIQPGDTLYSSVVIRFIGKIKHAARSQNASDLLVLNGLVDAHQLSPGQLI
                     KIPLEWIRPEYLHQVPSIYRISEKTTSTEKVSSPEHQPSIENGRHKTGSYYRTQIISQ
                     R"
     gene            10504..12342
                     /locus_tag="EYO68_10245"
     CDS             10504..12342
                     /locus_tag="EYO68_10245"
                     /inference="COORDINATES: protein motif:HMM:PF01520.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08219.1"
                     /translation="MKIKYFVFCLVLFGLSGLPASSSAKSTKQSNWQSFQLQYQSSET
                     SKMLVGWNGKRLVVKLKPLVGEGGYSLARRVLLSNFRSLKTIRKYSKTRRLYRNRFIT
                     FPLKVINGSIRSSALKAVFFKDKAGIGYWKHRVTFAWETTSMIAGLFTKEGIKAGHLV
                     RYNKMRNKGNILKKGDVIKIPWKWVSPELSLRPVSLKPPLKLKQDKSGKFYAHYQMKH
                     GETLYSSVVIRFTGRLLNDEVNQVANKLLKLNNISDAKLIQTRQKIRIPLGWLSEEYL
                     GSQTNDVSSRKTSVKKTNKKKTKKIKSSKAVASKKKLKRGKSTQKAVAQKTKSKRKNN
                     VHKIHVILDSGHGGRDPGASAGSRKNKDIIYEDEVVYDISKRMSKLFKKQGIIVHPTL
                     ADPNQKQPIRYLSHRHDRDEHLLVTPRYLTRNARIGVNMRVYLVNHIFKQLRKKKVPS
                     ENILFISLHGDALHSSLSGAMVYYPDHRLRRGRFILSSKIYRKRKEYISKLTYNPRDN
                     KYSEKLSKSFGKVIINQFRKLRLRTHRVSSAVRGYLYRKGIKTLPAVLRYSKVPTSVL
                     VEIANLNNRLDRRDLLKSKTRQQIAKAITNSVTAHFDRSSGLVAQR"
     gene            12360..12968
                     /locus_tag="EYO68_10250"
     CDS             12360..12968
                     /locus_tag="EYO68_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HIC08220.1"
                     /translation="MSLITSVVFDLGNVLIDWDPRYLYRKLIPTETDVEWFLENICDE
                     KWNAKHDAGYPFSDGIAEHSKKFPQYSELIRAWFSRWEEMLGDPLEEVVEVLSALKSS
                     GISLYILSNWSAETYPVAETRFDFLHWFDGKVISGETGMIKPDPNIYKLLMKTYGLTP
                     QKTVFIDDKSVNVEAANALGIHGIHYKNASKLRNDLRKLKLL"
     gene            13133..13459
                     /locus_tag="EYO68_10255"
     CDS             13133..13459
                     /locus_tag="EYO68_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08221.1"
                     /translation="MLAYSELLPWVKESSATSLLHIWAGVFFIVIFPMYAWDHIRGHA
                     DRLRNFTLVTASGILQFFTGLGLIISGIILLLYGAYALDLPREIHLVLTFVLAGSLIM
                     HKISRK"
     gene            13662..>15083
                     /locus_tag="EYO68_10260"
     CDS             13662..>15083
                     /locus_tag="EYO68_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIC08222.1"
                     /translation="MSSTKKIQLMDTTLRDGEQTQGVSFTPPEKANIAKTLLKKLRVD
                     RIEVTSARISEGELGSVQNIAEWAAENGFLECIEVLGFIDHKRSVDWIKESGSTVMNL
                     LTKGSENHCTNQLRKTLKEHLADIHNTIKYAHSKNLNVNVYLEDWSNGYHNSPEYVYE
                     MMDALKDEGINHFMLPDTLGVLSPDEVFSSLSDMIERFPWATIDFHPHNDYGLGVANV
                     YYAVKAGVRNIHCTMNCLGERAGNASLAEIAVVLHDKLGMELSIDESHLSPVSEMIES
                     FSGKRLAENTPIVGENVFTQTSGIHADGDKKGNLYHNPIVPERFGRKRSYALGKMSGK
                     ASLAKNLELLGIQLVDENLNKVLKRVVELGDSKKSLTAADLPFIITDVLETSDTQRIE
                     LLNCSITSGYNLRATATIHLKIDSKEYHEAGSGNGGYDAFMSAIKKILKRTHLDAPEL
                     VDYQVRIPRGGKSNALTEAIITWQ"
BASE COUNT         4679 a         2922 c         3070 g         4412 t
ORIGIN      
        1 tcacttattc acttggaacc agaagagtcc ccaactttaa tgtattaata tttttttact
       61 gatcagcaga attattattt aatgaatctt cggtaatttg tttggaagtt ttattgtcca
      121 tgaccaaatt aagctgttta actaactcca gtgaaccctc atatgatctt tgaatttttt
      181 gcattctcct tccactttgt atttcaaatt ttatcttttg cagacagtct ctcaattcct
      241 tgagttcttc tgaagaattt ggattactaa gttccggaca cttaagtgtc tgtttttctt
      301 gagccagtac tccatcattc tctacaaatt ctcctaaaaa cacaatactc actaaacaaa
      361 tcaggcagaa gaaaaaaaat cttgctccag aacatgtttt aatcgcaaaa gtcataaaac
      421 ggtaacgtat aatgttcagc ttgaaagttg aagccatttc tcttagtttt agaggttata
      481 atttaggatt gtttcacgaa atatttgaag tataaatgtt ttaaaagtat tatcaaagaa
      541 tgttaaaact atttattgca gccatttctg taggtctgat atattgggtt tggctcaaaa
      601 aaaaacaaat attttccaat cgcaataata aaattgaccc ctttatcact acaattgaag
      661 cgattgagat acaaacattt ttagattgga acacttcgca gtattgcctc gaatgtgatg
      721 gaatacgcta cggcaaacaa tttaagcaaa aaaaagcgcc agacttacca catgaggccg
      781 gatgtcgctg tgaggccacc aaattattct atacttctga tgacgttttt caaggtactt
      841 cacctgtttt aactcacaaa tctgctttgg gtgacctttc aacaaaagat gcattgctgc
      901 taaaaaacat cttgctcaaa attagaacca gcccaggaaa gggcaatttt tcagatttct
      961 tagaacaatt tgagataaac aatttttcag aaaatattcg ctccggagct atttctctag
     1021 tcgaacatgc ttttgaggca gagcaaaaca aggagtaaaa ttataaagaa aatgacaaga
     1081 aaagtctgac tcctgaattt aagaaccatc cttatctcta aaatctaaat ccgcggtaaa
     1141 aaaatacgaa tagtattcaa gcattatgaa aagacttgcc cagtttatca aatggtcatt
     1201 caggatagta ataattacag gaattatttc tggagcttct cttggggttt ttctgtttga
     1261 actcagtaaa actttgccac aaaatcttgg agatgaactc cacaaaagaa atgacgtatt
     1321 acctactgtc ctctatgacc gtgagggtaa tcagattgaa gagcttttca ttcaacggcg
     1381 catagtcata ccctatgaac agtttcctcc acacctgatt caggctctgg ttgccagtga
     1441 agatgcccgt tttttctctc atcttggaat tgatctactg agaattccca aagccttcct
     1501 tgccaatcta caagctggca gaattgttca aggtgccagc actctcaccc agcaaactgc
     1561 cagactgttt ctgttaagca gggaaaaaca gattattcga aaactccggg aaatgttgct
     1621 tgcgttccgg atggaaatgc aattttcgaa acaggacatt ctgtccctct atcttaataa
     1681 agtttatctt ggaaatgcgg aaggggttga ggcggcagcg cagggttatt ttggcaaaca
     1741 tgcttcagaa ctaaatctcg cagaaagtgc tttgctggtt ggaatacttc ctgcgccttc
     1801 tcgttatgca cctcacgtta atcccgattt tgccctgcag cggagaaatg tggttttgca
     1861 aagaatgcat caggaagggt tcatctcgga gcaagaattg cagaaaacaa ttcatacgcc
     1921 tatcagcctg attcgtattc atgattcgac cacagaggct acgtcctact atgtcgaaca
     1981 tgtacggcgt tatttgatta aaaaatacgg ttcaaaagtg ctttatcagg gtggtcttag
     2041 agtctattta gctatggact tgaactatca gacacatgct catgaggcct tgaggaaggg
     2101 gattctggag ttaacaaaac ggcagggatt tcggggtgca cttaacgaga atagtcctga
     2161 taacagtagc ttgacagaca actccacaga tgaaaataca ttaaaaattg attcgctgtt
     2221 tctcggaaac attgtcgctg gcgtggtcaa agaagtcagt aaggaaaatg tttcagtaga
     2281 actgggagaa tcgtacggaa ttctggaatg gagcaatatc agcacatgga aaaatggtaa
     2341 cattcttaaa gataaaacac ctatcaagat ttccaatccg gcagaaatat tttctgttgg
     2401 tgatgaagta caggtaagac ttgccgatta tgattcaaaa aacaattttt ttcgtctgca
     2461 gctctatcag gaacccttgg taaacggagc cttgttggca atgaacccaa agagcggaga
     2521 ggtactatca atgactggtg gttatcgtta cggagagagc gagtttaacc ggtccattca
     2581 agctttgaga caacctggat catcgttcaa accaatcgtt tattctgctg cccttgatgc
     2641 cggttttaca ctcagtagtg cactaataga cagtccaaga gcttatgcaa ccggtgcaga
     2701 gactgctgga gacgcggaaa tatggactcc gaaaaattat ggcgataaag tcatgggcaa
     2761 ggtttcattg cgtacagctc ttgtcaaaag tcttaacttg ccgactatcg gactttgtga
     2821 agaattgaaa ccgaagctag tcattgctta tagcaggcgt tttggtatca ctgcagaaat
     2881 gatggaaaat ttaaccacat gtctgggatc gttatcagtc actctgcagg aaatgattag
     2941 tgcctacggt gttttcgcaa atcagggacg attggttaaa ccaatctata ttctcagggt
     3001 ggaagatcag gatgggaata ttttggaatc aagtgtatca gagttaaaac aagtcacctc
     3061 tgaggaaact gctttcctgg tgagtaatgt tttgcaggat gtcgttcagc gtggaacagg
     3121 acggcgcgct agagcaattg gccgcccttc tgcaggaaaa accggaacca caaatgacag
     3181 cattgatgct tggtacatcg gctacattcc gcaattgctg acaggtgttt atgtaggatt
     3241 tgacaagcca agaagaatgg ggaaatcaga aactggatcc cgggcagcag ctccaatctg
     3301 gattaacttt atgaaaaatg cagttgcaaa tttacctaca gaacaatttc gacaaccacc
     3361 tggaatcaca accgttaaaa ttcataaatc cggaaggcgt gcaataccct gtgataaagc
     3421 aaaagatgta tacgaagaac actataaatc cggaacagag cctgtcctgg atctttcgca
     3481 atccggaatg tgtgttaaag caaaggaaac tgagaaaact gaaaaagagg aaagtgagcc
     3541 ggagttgtga ggataataat atgaggaagt tctaaaggtt caaggcattt acattgtcaa
     3601 agccttgaac cttgagctga tattcttagt tcaaagattt tttgagcttg cttccagctt
     3661 taaactttgc acctttggat gccgggattt gtattacctg agatggattt tgaggattgc
     3721 gacctacacg ggctgcacga tgggacacag aaaatgtgcc gaatccaatt aacgttagtg
     3781 agtctccgcg ctggagagtt ttttgaactg ttgctgtaaa ggacttcaga aaacgatctg
     3841 cgtcggcctt tgtaaggcct gtatctgctg ctagagcatt aataagatca gcttttgtca
     3901 ttgtcattct ctcagaaaaa gattagtccg gatgtttttg tataacaagc ctccacctat
     3961 taaacacatc ctattaataa tatccaattc acaccatgca aattagactg tttcgcaaac
     4021 tatttcaaac tatttagttc tacgatagta agaaagctac aagctttcaa taaacttgtc
     4081 aaggattttt aagaaaaaaa tgaatttttt tcaaaaatta tataaatttg ttgtttttcc
     4141 gggtatttca gcaggaattc gtacctctgc gagaaatctc aacggcatgg aaaaattctt
     4201 tattttccac gatcagggaa ctttttcctt tattttcgca gcttcagtgt tgcttcacat
     4261 agtgctggta ataattacta tggcaatttc tgagttatgg gtacaggaac tgccacccat
     4321 tagggcaaaa atcgaggtgc gttttgcaaa aattccttct gagccagctc caattgaaaa
     4381 acctaagccg gttctaaaga aaatagaaaa tgagtggcag ccaaagttga gcaatcttgt
     4441 cccaaaaaag cctgtgctgg aaaaacctgt tctaaaagat acacttaaaa aatcaacact
     4501 atctaaacct gaaataactc aacctgaagc tccgcgtctg aaaatgtcag aaccgcaatt
     4561 agctccttca gttcctaccg cgaaactaaa caagatctcg gcaccaaaga aacccttact
     4621 acttccagtg gactctctgg aaaaatctcc tctaattcca agtttgccaa aactaagcaa
     4681 agatcctgtg cctctttctc ctctgaggtc caaaaaatca aaacttaatc cgagtcagtt
     4741 ggtattgcca aaaatatcac tggaagacat tacacctaaa aaaataaatg cacccaagat
     4801 aattaatatg cctaaaaagt taaataaacc taaaatcaaa aattcacaaa aattttcaaa
     4861 accattaaat cttcctgttc gggaattgcc ggaaacaatg cagtctgctt tacaaaaagt
     4921 accctcaatt cctcaagttc aaccttcaga caaacttggg accaacctta gggaaatatt
     4981 tcctgacaaa ataaagttcg atgaacctct tccggatttg ggtattcctg agcaagctga
     5041 cgagacgaaa ttaaaagaaa tacctgatac cgtcaatttg cagcgtaaaa aattagcaca
     5101 actggctgga gaggaatata atctgcacat tcgtacaagg attatcccta aactcggcag
     5161 ttattcttca gagttgtacg ttcgcatccg gctcagaatt ataacaacag gtaaaattat
     5221 tgactatgaa atcattaaaa aaagcggatt tgctgctttc gataaagctg cagagcttgc
     5281 tgtacggaac gcgcttctgg atcctctacc caatgctctt gcagaaaatc ctccctacat
     5341 cgttacaatt cggattattc cccaaagtaa aaaatagaat gcaatcatag ctcatgcact
     5401 cagtaattta tatttttgca cttcgaaaat ttcgaattta cttcactatt ttcttcctct
     5461 gtttcagcac tttagttcaa gcccaaactt attccagcga ggagatagtt atcagtggac
     5521 tggggtttgc caggtttagt gtagctttag ttccggaaaa gagttttaca gatcatatgg
     5581 atgctaaacg ctggctgagg ataattgacc gaaacctttg ctggagtggt gtgtttctgg
     5641 tgacagattc acgatatagg acttgccgga ctgcaggtgg aaatcaggtc gatatgaaaa
     5701 tcatgctcaa gctgaaaggt acagttacag acaacggtga tctggttcca aaatatttaa
     5761 tactgacggt tgcagataat gatggagcgc cgctgtttcc cctagaattg ccaatacgaa
     5821 aaaatcgatt tcgggaagct gaattaatgg atttgattaa cgaaatgtcg gtacaactca
     5881 caggacttaa gggtattctg ggaagtacga tcgcatttac acttaaacaa cctaaacgtc
     5941 gaaaaattat tgcaagaatt aatacacacg ggaaaaagtt ggcggctgtt tcgaggaaca
     6001 aatatatcaa cttattgcca agttggagtc cgaaaggcga tgccattgtt tacacgacac
     6061 taagtcgacg cggcactgca attatgttta acgattgtct aaaggttgtc ggctgcaaat
     6121 atcgtcctgt aaaactgctg agttcaaata tttctgctgc acgtatttca aaaaattttc
     6181 tcagtgtcag cggtgggaca tggttttcaa atggacataa gctgattgtg actctcagcc
     6241 gtaaaggcaa ttctgatttg tacgaatttg acaggctgaa gagaaaagta atacggctga
     6301 ctactcaccg ggcaattgat acagtaccag atttgtcgcc ggataatcag cacttaattt
     6361 tcgtttcaga ccgctctgga cacgaacaaa tttattattt gaagttgggc acaaaaattc
     6421 cctttcaact tacatttggt cgaggcagca gcagtgaccc ggtttggtct ccggatggta
     6481 ctcttatcgc tttttctaaa atcagctcag gacacagcca aattcatttg atggatccat
     6541 ttacagggga agataacttt ctaacaagag gccgtttcaa ttcagagcaa cctttctggt
     6601 ctccggatgg acggcaaatc gcttttgttt cttcttcaac aggagttgat aaattataca
     6661 ttatgtttat tgatgggacg ggcaggcgac gtttgacacg tacgccgaga gattttgaag
     6721 agggtggtcc cagttggaca gcaaggaagt tttgatgcaa tttgaagatt ggcaaactgc
     6781 accagatccc aaagttatcc ggaaagaaaa gcagaaagcc agggaacttc ggaaatcgca
     6841 gtggtggaaa aaccgcagag cctgcaacag ttgttattac tgtgaaagcc ctacgccagc
     6901 aaaaaaattg accatggatc atgtggtgcc cttggcacgc ggcggaagga gtataaaatc
     6961 taatttagtg ccctgttgca agtcttgtaa taatcagaaa aagaatttat taccaataga
     7021 gtggaaagaa tatttagcaa ttattggtaa aaaaaaggag taattttttt tgaaaaaaat
     7081 attttttctt aacacctgta tttcttgcct tttttgtttt tcacctccct ttttgaattt
     7141 tttcatttat ttttaacttt acaaaagcag ttttttttgt tagcattgcc ttcacattgt
     7201 gtttggttgt atttaaagag aattgaaatc aaacaaagat gttgagcaaa caaacgatga
     7261 acttgtgaac agacaatttt ttagtttatt gacaaataaa tatcaggatc agggtaagat
     7321 gaaaaataaa tttaatcgac agggcaaccg atttggatct cgtgagaaga gcaaggcaaa
     7381 aggcggtgca aactcttcca aaggtcatcc caccagcaaa gaaattgagc ttgctacgca
     7441 ggcttatctc tcagaaggag gaaaaattga tcgggtcgca ctgaatcacg gtaacgccga
     7501 gaaagaggtg ttcagggcaa acttggaagc tgattctcgt ttggggatgg gaactttggt
     7561 ggatgattct tgggactgct gggaaaacaa ggattacgat tcaaacccca ctggttcagg
     7621 aatataattg ttgcgttgct tgctgcatga gaattgacaa attttcagcc acgtagacct
     7681 tcttcctgaa aacattaact tcaactgaat cccgcgcagg tattagcgtt cggtagtggt
     7741 gaatatgcag aaatgtgatg atttaattca ggtccactga aggtctgaga agttctcact
     7801 gcatcttttc ttttcaaaaa tcaaaaaaga acaattgggg cttgtaatat cagtatccat
     7861 gcgctacact tccacttcat gtttcatgga aaagaggttt cttttctaaa ttctaacatt
     7921 gaggtacacg taaaaagtcg aaacttataa aagcttgagg ccagagtcgc agaagtcata
     7981 gaaaaattat taaatcaatt ctctgtgttt ttcaaaactc tgcgttgagt ccagtatttt
     8041 gctgttttag actttttacg agatcattat ttaagcttgg agaatgcaaa tgcgaaaaat
     8101 gttttttttg atcgtgaccg tatcaatctt gataccattg tcagcccctg catccacaga
     8161 acaacttggt ataggagtct tcgattatat tatttcccga actgatttca atgttgaaac
     8221 ctgtgaggcc tacagttgta actatgtctc cactgacaaa acaattaaaa tggagttgag
     8281 gcgtgcaaac ttagcggagc ataatggcaa agatctaaat ttcctgctag atattgactt
     8341 tgacagtaaa ttatttaaat ctggtttgct tgttcggtat tacaacgaca aagacctgat
     8401 acgtgtattt atcaagagat catctataac aattgaagct acaagaaggg atgaaattac
     8461 aactctacag agttggctta aaaatgtttt gagtcttgta aaataatctc tatgccatcc
     8521 atttttccta aaaaatatct gtcgaataaa tatttcatgt ttgggtcaga ttagtattta
     8581 ttttctgacc caagtctcgt ttctcatccc tcgttatttt tcaatcatat ttcatagtta
     8641 ttttcatgga tattctgtcc acaggcctaa ctttgcttct catcatggat cctctgggaa
     8701 acattccctt gttcctctcc gtcttaaaaa ctgttgacga tgagtcccgc aaacgtaaaa
     8761 tattaatccg cgaattatgt tttgctttgc ttgtgctatt gatatttttg tttgtcggcc
     8821 aatatctttt acaatggttg aatctgaggc aggaagctgt gagcatagct ggtggaatag
     8881 tgctcttttt gatttctctg cgaatgatat tccccaccga aaagggtata atgggtgaaa
     8941 tgcctgcagg ggagccgttt tttgttccgc ttgcggttcc tttgctggca ggaccctcaa
     9001 cattggcgat gcttatttta cttgccagat ctcaaccaga ccgaatcttt gaatggttga
     9061 ttgcggtttt aggggcatgg gttgttacaa gtctgattat gctttcctca accaaactcc
     9121 acaaactcct tggtgtaagg ggactcattg cagtggaaag gctgatggga atggttttag
     9181 tggcaatttc tgtgcagatg ctgttggacg gaatcactac atacctaagc gttattccat
     9241 cactttgatt tgtgcctcat tagacatttt ctttccttga aatgacttct tctctctcct
     9301 caaatttcat tccacctctg ttaatatttt ctcaacatcc cctctgtttt ttgctgctga
     9361 gccaactggc acagtttttg ttgtgtttag ggaagagcct tagttcagtc gatttagact
     9421 aaaggttcag taattcaggt gagaaccatc ctgaattatg aaaaatacaa catactaatc
     9481 gcaaaaacaa tgccgtttaa atatttacta attgcaactt ttggtgcgct gctttttatc
     9541 aactacagtg gtacagcagc aactaaggct gcagacttat cacaaaaaaa agcagaattt
     9601 ctgccgctgg catcattttt atatgctaat cgtgatccaa tgcgtgttgg ctggtataac
     9661 acgtccagta tgccgttgga aaaagacttt aaaacagatg caaaaaggcc cgttggttca
     9721 ttttttgtgc agctcaagcc acgaaaaaat gaaggagaat atcagcttgc acttcgggtt
     9781 ttaagagacg gctcccatca gtttttactt atacgggaat ttactaacgg caacacttta
     9841 aatcataaac gctatttaac aattccattt aagttactga ttggtgcaat tcagggggag
     9901 gctttacgtt ctctttttcc gaacgatcat gttgaattcg gaggttggcg gcatcaggtt
     9961 acttatgtct gggagactcc ggaattacta atgaaatctt ttaccaggtc cggaacacag
    10021 accatactgc agaaagattt catgcaaggt gaggaatata ccattccatg gaaaaattta
    10081 cgttctgatc tagaactgga accgttagcc gtgcgtgatc cgcttttcat cagaaaagac
    10141 aagtctggtc tgcgctatgc tttttatcga attcaacctg gagatacgct ttattcctca
    10201 gtagtaatcc gttttattgg aaaaataaag catgctgcac gcagtcaaaa tgccagtgat
    10261 ttattggttt tgaacggact tgtggacgca caccaacttt caccgggcca attgataaaa
    10321 attcccctgg agtggatccg tccggaatat ttgcaccaag tgccgagtat ttaccgtatt
    10381 tccgagaaaa caactagtac agaaaaagtg agttcacctg aacaccagcc atcaatcgaa
    10441 aacgggcgac ataaaaccgg gagctattac cggacacaga tcatcagcca acgctagaat
    10501 attatgaaga taaaatattt cgtcttttgt cttgttctgt tcggtttgtc cggactgccg
    10561 gcaagttctt ctgcaaaatc tacaaaacaa tctaactggc aaagcttcca gttgcagtat
    10621 caaagttcgg aaacatcaaa aatgctggtt ggttggaatg gaaagagact cgttgtcaaa
    10681 ctgaaaccgc tggttggaga aggaggctat agtttagcca gaagagtcct gttatcgaat
    10741 tttagaagcc tcaaaacaat acgtaaatac agtaaaaccc gtcgtctgta ccggaaccgc
    10801 ttcataactt ttccactcaa ggtcattaac ggcagtatcc gcagttctgc acttaaagct
    10861 gtattcttca aagacaaagc tggtatagga tattggaaac accgtgttac ctttgcctgg
    10921 gaaacaacca gtatgattgc aggtttgttc actaaggagg ggatcaaggc aggacattta
    10981 gttcgttaca ataaaatgcg caataaaggt aacatactca aaaaaggaga tgtcatcaag
    11041 atcccctgga aatgggtcag tccggaattg tcacttcgtc cagtttcctt aaaaccaccg
    11101 ttgaaactta aacaggacaa atcaggtaaa ttttatgctc attatcaaat gaaacacgga
    11161 gagacacttt actcttctgt cgtgattcgt tttactggac gcctgttaaa cgatgaggta
    11221 aaccaggttg caaacaaact gctgaaatta aataatattt ctgatgcaaa actaattcaa
    11281 actcggcaaa aaattcgaat tcctctaggg tggttgagtg aagaatatct tggttctcaa
    11341 actaatgatg tatcttccag aaagacttct gttaaaaaaa caaataaaaa gaaaacaaaa
    11401 aaaataaaat cctcaaaagc tgttgcttcg aaaaagaaac ttaaacgtgg caagtcaaca
    11461 cagaaagcag ttgctcagaa aacaaaatct aaacgaaaaa ataatgttca taaaatacat
    11521 gtgattctcg actccggcca cggcggacgt gatccgggag cttctgcagg atcacgaaaa
    11581 aataaagaca ttatttacga agatgaggtt gtttacgata tcagcaagcg aatgtcaaag
    11641 ttgtttaaga agcagggtat tatagtacat cctacacttg cagatcccaa ccaaaaacaa
    11701 ccgatacgtt atttatctca tcgtcatgac cgagacgaac atcttttagt cacaccgcgt
    11761 tatttgacgc gcaatgcacg tattggtgtt aatatgcgcg tttaccttgt aaaccacatt
    11821 tttaaacaat tacgaaagaa aaaagtgcca tccgagaata ttttgtttat cagtcttcat
    11881 ggtgacgcac ttcattcctc actgagtgga gcaatggttt attatccgga tcatcgcttg
    11941 cggaggggac gttttatatt gagtagtaaa atttatcgca aacgcaaaga gtacatttca
    12001 aaattaacat ataatccgcg cgacaacaaa tattcagaaa aactaagcaa atctttcggc
    12061 aaagttatta taaatcagtt tcgtaaatta agattgcgga cacatcgtgt ttcctcagca
    12121 gtaaggggct atctgtatcg aaaaggtata aaaacgcttc cggccgtgct gcgctacagt
    12181 aaggtgccga catctgtttt agtagaaatt gcgaatctta ataatagact cgatcggcgt
    12241 gatttgctaa aatctaaaac tcgacagcaa attgcaaaag ccattacaaa ttctgttact
    12301 gcccattttg accgttcaag cggtcttgtc gctcagcgtt agtcaacaaa atactattta
    12361 tgtctttgat cacctctgtc gttttcgacc ttgggaacgt cttgattgac tgggatccgc
    12421 gttatcttta cagaaaatta attccaactg aaacagatgt tgaatggttt ttggagaata
    12481 tttgtgatga aaagtggaat gccaaacatg acgcaggcta ccctttttca gacggtattg
    12541 ccgaacattc taaaaaattc ccacaatact ctgagttgat tcgagcctgg tttagtcgct
    12601 gggaagaaat gctgggagac cctttggaag aggttgttga agttttatct gctctaaaat
    12661 caagtgggat ttcactatac atcctcagca actggtcagc agaaacttat ccagttgcgg
    12721 aaacacgatt cgatttcctg cactggtttg acggtaaagt catttccggc gaaaccggaa
    12781 tgataaaacc tgatccgaat atttacaaat tattgatgaa aacttatgga ttaactccgc
    12841 aaaaaactgt ttttatagat gataaatccg tcaatgttga agctgccaat gcattaggaa
    12901 tacacggaat tcattacaaa aatgcctcaa agctccggaa cgacttacga aagctgaaat
    12961 tgctctaact tgtgattcct gctcaccaag ttgccttttt ttcacattta atctattctg
    13021 tcctctttat cttttccatt tctggaattg ccagggaact tattatttaa ttgcggaaac
    13081 cttgagaaaa cttgttgttt taagctgtgt ttttttgatt atttccggca taatgttggc
    13141 atactcagaa ctgttgccgt gggtaaaaga gtcttctgca acatccttac tgcatatctg
    13201 ggcaggcgtg ttctttattg ttattttccc catgtatgca tgggatcaca ttcggggaca
    13261 tgcagatcgt ctgcgaaatt ttactctggt tacggcttcc ggaatactcc agttttttac
    13321 cggcttggga ttgattattt ccggaattat attacttctt tatggtgcat atgctctcga
    13381 tttaccccgt gaaattcatc tggtactaac gtttgtattg gcaggaagtc ttattatgca
    13441 taaaatttct agaaaataaa ttactgggtt ttaatcaagc ttttcgaaca ctcgacagtc
    13501 acgagtttta ttgtgtcatc ccgaacgtat gtgagggatc ttataagata acagtacttt
    13561 gtcatatctc ttatttcggt cgagatgaca taccgcagtc aataatatca gtatattacg
    13621 agaattcatg actcatgagt tcgaacagaa cagcacatac tatgtcttct actaaaaaaa
    13681 ttcaattaat ggacaccacg ctgagggatg gagagcagac ccagggagtc tcattcacac
    13741 cacctgagaa ggccaatata gccaaaacac tgttgaaaaa gctgcgtgtt gatcgaatag
    13801 aagtcacttc cgcaaggatt tctgaaggtg aattaggttc tgtacaaaac attgcagagt
    13861 gggctgcgga aaatggtttt ttggaatgta tcgaggtttt gggattcatc gaccacaaac
    13921 gaagtgttga ctggattaaa gaatcaggtt ctaccgtcat gaacctgtta accaaaggca
    13981 gcgaaaatca ctgtacaaat caactcagaa aaaccttaaa agaacatctt gccgacatcc
    14041 ataatactat aaaatatgca cattcaaaaa acctgaatgt aaacgtttat cttgaagact
    14101 ggtcgaacgg atatcacaac agtccggaat atgtttacga gatgatggac gcactgaagg
    14161 atgagggcat caatcatttc atgcttccgg atacgcttgg cgtgttgtct cccgatgaag
    14221 tattttccag cctttcggat atgattgaac gtttcccctg ggcaactata gattttcatc
    14281 cgcataatga ttacggtttg ggagtggcaa atgtgtatta tgcagtcaaa gctggagtga
    14341 gaaacatcca ctgcacaatg aattgtcttg gtgagcgtgc aggaaatgct tcacttgcag
    14401 aaattgccgt tgttctgcat gataaactcg gtatggaact ctcgattgac gaatctcatc
    14461 tttcgccagt gagtgaaatg atagagagtt tttccggaaa aaggctggct gaaaatacgc
    14521 caattgttgg agaaaatgtt tttactcaaa ccagtggcat ccatgcagat ggagacaaaa
    14581 aggggaattt atatcacaac ccgatagtgc cggaacgttt tgggcgtaaa agaagctatg
    14641 ctcttggaaa aatgagcgga aaagcctctc ttgcaaaaaa cctggaactg ctgggcatac
    14701 aactcgttga tgaaaactta aataaagttc tcaaacgtgt tgtagagctt ggtgattcaa
    14761 aaaaatcgtt gactgcagca gatttaccgt tcattatcac ggatgtgctt gagaccagtg
    14821 atactcagcg aattgagttg cttaactgct caattacaag tgggtataat ttaagagcca
    14881 cggccacaat tcaccttaaa attgattcga aagagtatca tgaagcagga agtggaaatg
    14941 gtggatatga tgcgtttatg agcgctatca aaaaaatatt gaaaaggacc caccttgatg
    15001 ctcctgagtt agtggattat caagtcagga ttccacgcgg cggaaaatcg aatgctctca
    15061 cagaagcaat tatcacctgg cag
//
LOCUS       DTVD01000279            4062 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8004_length_4062_cov_481.006239, whole genome
            shotgun sequence.
ACCESSION   DTVD01000279 DTVD01000000
VERSION     DTVD01000279.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8004_length_4062_cov_481.006239"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(348..1676)
                     /locus_tag="EYO68_10265"
     CDS             complement(348..1676)
                     /locus_tag="EYO68_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIC08223.1"
                     /translation="MTEGPVGPSLIRLAVPMIFGLVSIMLFNLVDTFYISLIGVRELV
                     AISFTFPVTFTLMSFSFGIGIGAAAVISRAIGEGDQHRVQRLTTDSLLLVALIIICVA
                     GLSFFALDSIFTLMGASTESLPLIKEYMIPWLSGVVFVVIPIVGNSAIRATGDTKTPS
                     WIMMIAAGVNAVLDPLLIFGYGPFPELGIQGAAIATVISYISIMIAGLWVLGIRERML
                     TTNWPGIAEILRSWKALLYIGIPAMVTQLLFPFSNAVLTRIAADLGDATVAAFGVGTR
                     IESIAMIGSMALSSVLIPFIGQNLGAGKFGRIKDASRFSLRFAFVWGTTAWVILALFS
                     GGIAWAFSDDPEIQNFIQQFFWIVPFGFAFHGISQLVSASCNALHRPFHSTTINILRL
                     FLFLIPLVYLGSQIWGTTGFFFGIALGNLATGGVAWYWFRSSILPGALIE"
     gene            1953..3209
                     /locus_tag="EYO68_10270"
     CDS             1953..3209
                     /locus_tag="EYO68_10270"
                     /inference="COORDINATES: protein motif:HMM:PF05762.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIC08224.1"
                     /translation="MSTANRHKSASVHEMPESDSLFDDLALRRELSANLVGFCRYLRQ
                     NGLRTGMGEQMDALRALEEVDLKHETAFKMALRTTLAKSTQEQEIFDEHYHSFWYIWE
                     SAENLQQRFKAKKEEPAAVVVDARPKKESFMSIGDWLGGTETAEEDKEAAGYSPFETD
                     SERDFSSFRAEELSEVMHLINEIGKALATRFNRRTINSKKRGLIDLRRTMRLSLRRGG
                     EIIDLAHSRRRRQRLKLVLLCDVSKSMDLYSRFLIQFIYAFQNVYRRIETFVFSTSLH
                     RITETLRKEELYSALEQLSATVPDWSGGTKIGASFKQFVEQYGLKLVDSQTVVLIISD
                     GWDTGEVDLLEDSMHFLHKHARNVIWLNPLKGSPGYQPATRGMQAALPHIDIFASAHN
                     LNSLRNLVHQLARIQGGQSSRLAIGV"
     gene            3291..3815
                     /locus_tag="EYO68_10275"
     CDS             3291..3815
                     /locus_tag="EYO68_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIC08225.1"
                     /translation="MPLYTLDEATPEFTETTFIAPDASLIGRVRIGAFSSVWFNAVLR
                     GDMEHISIGDETSFQDLSMGHSDPGFPLIIGNRVTVGHHCVMHGCEVEDDCLIGMGAI
                     LMNGVKIGRGSIVGAGAVVLEGTEVPPFSMIAGSPAKVRKTYEESILEDIRGMSQIYV
                     KRTIRYLEQNIGER"
BASE COUNT         1192 a          850 c          958 g         1062 t
ORIGIN      
        1 gaagttattc tctgaaagtt gcaggagttt taagaaaaac atttttcctc attgccggca
       61 cccagctcga cttgtctatt gattaaaatg cagcagcggc agggactcgg catgatgtcg
      121 ctttagtttc ttgtttaaga ggtagcccca gacggatctt gtgctgagtc gaaaggctgt
      181 tccggggaaa tgcggttggg ccagcaaaaa ctactgtagc tcagcatatt catcatcagt
      241 taaatatttt ggcttccagt tttcaaaact ggaagcctct ataaaactga acatcgttta
      301 attatattgc attgtcgcat agtgtaaagt gacatttttt ttcaaggcta ttctattaag
      361 gcgccaggca aaattgagct tcggaaccaa taccaggcta ctccgccggt ggccagattt
      421 ccaagtgcga tgccgaaaaa gaatccggtt gtgccccaga tttgtgatcc cagataaacc
      481 aggggaatta ggaagagaaa gagccgcagg atattgattg tagttgagtg aaaaggccga
      541 tggagtgcgt tgcatgaggc actgaccagc tgtgatatac cgtgaaaggc aaaaccgaaa
      601 ggaacgatcc agaaaaattg ctgtataaag ttttgaattt caggatcatc tgaaaaggcc
      661 caggcaatgc cacctgaaaa tagtgcaagg attacccatg ctgtagtacc ccaaacaaaa
      721 gcaaatcgca gcgaaaaacg gcttgcatct tttatccttc cgaactttcc tgcacctaaa
      781 ttttgtccaa taaaaggaat gagtaccgat gaaagtgcca ttgatccgat cattgcaata
      841 gattctatgc gtgtgcccac cccaaaagct gcgactgttg catcgccaag atctgctgca
      901 atacgagtta aaaccgcatt tgagaatgga aaaagaagtt gggttaccat ggcaggaata
      961 ccgatataaa gtaaggcttt ccaggaacgc aggatttcag caattcccgg ccagtttgtg
     1021 gtcagcatcc tctcccgtat tccaagtacc cataaacctg caatcatgat actgatataa
     1081 gaaataacag tggcaatggc tgcaccctga attccgagtt ccggaaatgg accgtagcca
     1141 aaaatcaaaa gcggatccag cacggcatta actcccgcgg cgatcatcat aatccaactg
     1201 ggggtttttg tgtctcctgt agcccgaatc gcactatttc caactattgg tatcactaca
     1261 aatactactc ctgacaacca aggaatcatg tactccttga ttaacggcaa cgattctgtt
     1321 gatgctccca tcagtgtgaa aatagagtcg agcgcaaaaa aactaagtcc ggcgacacag
     1381 atgataatta aggcaaccag gagcagactg tctgtagtca gtcgctgtac tctgtgctga
     1441 tctccttcac cgatagcacg tgaaataacg gcagcagcac caattcctat gccaaaggaa
     1501 aagctcatca aagtaaaagt aaccgggaaa gtaaaactga ttgcaaccag ttcccggact
     1561 ccaatcaggc tgatgtaaaa tgtatccacc aggttgaaaa gcataattga gacaagtccg
     1621 aaaatcattg gaactgccag acgaatcaag gaagggccaa ctggaccttc agtcatgcgc
     1681 tttctgactg aattttgttt ggaagaatta gcagaaggaa aggatgtcaa taaaacctga
     1741 aagtgcaatg attgggagtt atctttctta aaacagtgag gaaataagat agtagcatat
     1801 tattcactca actcacaact gggattcgct tttgggtcag gaatcttctt tattgaaata
     1861 actattaaag gccggaatcg gcttgattgc ccgatatatg tgtgggagcc tgtacgcata
     1921 ctgtgattca gaacaatttt ttgtaaaacc tcatgtccac cgcaaaccga cacaaatctg
     1981 catccgtaca tgaaatgcca gagtctgatt cgttgtttga cgacctggca ctgcgtcggg
     2041 aattgagtgc aaatctggtt ggattctgcc gttatctacg tcagaacgga cttcgtaccg
     2101 gaatgggtga gcagatggat gcactgcgtg ctctggagga ggtggattta aagcatgaaa
     2161 ctgccttcaa aatggcgctc cgcacaactt tggccaagag cactcaggag caggaaatat
     2221 ttgatgagca ctaccatagt ttctggtata tctgggaaag tgcagaaaat ctccaacagc
     2281 gtttcaaggc aaaaaaagag gaacctgccg cagtagtagt ggatgcacgt ccaaaaaaag
     2341 aatcattcat gtcaatcggc gactggctgg gaggcacaga gacagccgaa gaggacaaag
     2401 aagctgcagg ctacagtcct tttgagacag attctgaacg tgatttcagt tcttttcgtg
     2461 cagaggagct tagcgaagta atgcacctca tcaacgaaat aggtaaggcg ctggcaactc
     2521 gtttcaaccg ccggacaata aattcaaaaa aacggggact tatagattta cgcagaacaa
     2581 tgcgcttaag tttgcgccgt ggaggagaaa ttattgattt ggcacattcc cggcgcaggc
     2641 gccaacgtct gaaattggtg ctgttatgtg acgtgagtaa atcgatggac ctttacagtc
     2701 gtttcctgat ccagttcatc tatgcttttc agaatgttta tcgacgtatt gaaaccttcg
     2761 ttttcagtac ttcactgcac cgaattacgg aaactttacg caaggaagaa ttgtactctg
     2821 cgctagaaca actttcagca acagtcccgg actggtccgg cggtacaaaa attggcgctt
     2881 cttttaagca atttgtcgaa cagtacggtc tcaagctggt tgattctcaa actgtagtgc
     2941 ttatcatcag tgatggttgg gacacaggtg aagtagattt gctggaagac agtatgcact
     3001 ttttacataa acatgcccgt aatgtgatat ggctcaatcc actcaaggga agtcccggat
     3061 accaacctgc tacccgtgga atgcaggcgg ctttgcctca tattgatatt tttgcttcgg
     3121 cccacaatct caacagtctc aggaatcttg ttcaccagtt ggctcgtatc cagggtggac
     3181 aaagcagccg cttggcaatc ggagtttgat actaagcagt cctgaaattg aattgaaaac
     3241 agtaatttgt caaagttgac agcaatctaa cacattgttc agtaagtact atgcctttat
     3301 acacacttga tgaagccact ccagaattta cagaaacgac tttcattgca ccagatgcca
     3361 gtcttatagg aagagtacgg attggtgcat tttcttcagt ttggtttaac gcagttctcc
     3421 gcggagatat ggaacacatc agcattggcg acgaaaccag ttttcaggat ttaagcatgg
     3481 gacactccga tcccggattt ccgctgatta ttggaaatcg ggtgacggta ggacatcact
     3541 gcgtaatgca cggctgcgaa gttgaagacg actgcctgat tggaatgggt gcgattttga
     3601 tgaatggagt aaaaattggc cggggcagta tagtcggggc aggtgcagtt gtgctggaag
     3661 gaacggaggt tcctccattt tcaatgattg caggttctcc tgcaaaagtg cgtaaaactt
     3721 atgaagaaag cattttagag gatatcagag gtatgtcgca aatttatgta aagcgcacga
     3781 tacgatacct tgaacaaaat attggtgaga gatagtggtt tcctgagttg gaattctgaa
     3841 tcaggctgca atttcgttgg attcccgagc ccggagaatt gcggtaaatg cagtttgcag
     3901 ttcattggga gtaagttctt caagttcagt atgttcactg ttcagggttc tgtttatgaa
     3961 ctgaagctga ggttctttgg caacataacc caattgattc agttgtcgat ccaaggtttc
     4021 aaacaccctg ttacgtaact gctcattggt gtttttatct tt
//
LOCUS       DTVD01000280           15081 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_800_length_15081_cov_525.276521, whole genome
            shotgun sequence.
ACCESSION   DTVD01000280 DTVD01000000
VERSION     DTVD01000280.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_15081_cov_525.276521"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="cbiT"
                     /locus_tag="EYO68_10280"
     CDS             <1..473
                     /gene="cbiT"
                     /locus_tag="EYO68_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HIC08226.1"
                     /translation="LWDVGAGCGSVAIEWMRTSPRCRAVAIEKSKSRLKLIEQNSQEL
                     GVPMLQIISGSAPEVLADLPAPDAVFIGGGLSSGNLLQTCWNALKPGGRLVANAVTLE
                     GEQKLLQWQNENAGKSGASGDLTRLSISRAEKIGKFQGWKEMRSVIQLAVIKGY"
     gene            519..881
                     /locus_tag="EYO68_10285"
     CDS             519..881
                     /locus_tag="EYO68_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIC08227.1"
                     /translation="MKFQNKFFGSAIEVHKILGPGLLESAYEECLCRELETRNISFER
                     QLPLPLEYKGKPLDCGYRLDLLVSNTIVVELKAVSLIEPIHEAQLLTYMKLGGWKLGL
                     LINFNVPLLKNGIRRRIL"
     gene            909..1634
                     /gene="cobI"
                     /locus_tag="EYO68_10290"
     CDS             909..1634
                     /gene="cobI"
                     /locus_tag="EYO68_10290"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIC08228.1"
                     /translation="MKNKSGTLYGIGVGPGDPELLTLKAHRLINECDVLAYPAPEVGD
                     GLALQIVSPLLTNDPDLFPLRLPIAVSPFPAQQAYDQAAKILEIPLESGKSVAVICEG
                     DPFFYGSFMYLFERLATSFKTEIVPGVSSPMACAAVLKTPLVSRNEILTILPGPLEEK
                     ELEQRLLDTDSAAIMKVGRHLPKIRRVLRHLRLEHCAQYVEHATMHNQQIIPIENLEA
                     QKVPYFSMILVHKNTGYSSNGVK"
     gene            1631..3556
                     /gene="cobJ"
                     /locus_tag="EYO68_10295"
     CDS             1631..3556
                     /gene="cobJ"
                     /locus_tag="EYO68_10295"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIC08229.1"
                     /translation="MSQQIAVFLLGISAFPVAKKIAAELGAELHGKADRISQVSSEPE
                     ADVFFTDAMEHLSTLFSEGVAIVGVCASAVLIRGVAGCLQNKLNEPPLIAVAEDGSAV
                     VPLLGGHHGANDLAHQIADILGISPAVTTAGDLRFGIALDEPPEGFVLSNPEDVKHFT
                     AELLSGEYVSLSSGDKPAAPDYMPGFYKWLQDSRLPFSENAKLRISLAAKPISGNAEH
                     LVFQIAPDFSVKNIAVGVGCERGTDPEELITLVLNTLHENDISPERVAVVVSLDLKAD
                     EPAVHAVAKNLECSVRFFDAATLEALTPKLKNPSEIVFQEVGCHGVAEGAALAAVGDS
                     GILLVPKVKSSGMSCAGRGTCAIAESAEFLDPQMIGRAQGTLFIVGTGPGTPQWRLPE
                     SENMLRKATDWVGYGLYLDLISDLHNGQKQHRFDLGQEEVRVRHALKLAAQGKTVALI
                     SSGDPGIYAMSSLVFELLETGKSGASAKSCAKDNSVAEWQRIHVEATPGISALQAASA
                     RIGAPLGHDFCTISLSDLLTPWETIERRIRAAAEGDFVIAFYNPVSRRRTTQLVRAQE
                     ILLKHRPAETPVIFARNLGRDEEKVKVVSLENLDPAQVDMLTVVLVGSSQTRLLELPN
                     GEVKIYTPRGYDQKSKN"
     gene            3578..4351
                     /gene="cobM"
                     /locus_tag="EYO68_10300"
     CDS             3578..4351
                     /gene="cobM"
                     /locus_tag="EYO68_10300"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIC08230.1"
                     /translation="MTVHFIGAGPGAPDLITIRGRDLIAKCPVCLYAGSLVPEEVVAF
                     APASALVLDTAPLNLEEIITEIQTAHEAGKDVARVHSGDPSIYGATAEQMRRLDELDI
                     PYDVTPGVPAFAAAAAELKQELTLPEVSQTIIATRTTMHASAMPEGEELAKLAAIGAT
                     LVIHLSVRNLKFVQDELIPHYGKDCPVVVAYRVTWPNQQFIHGTLANIREKLRKSKIS
                     RTALILVGRVFDAKNFRDSALYDPQHVHVLRPKKKSVKK"
     gene            4463..6937
                     /locus_tag="EYO68_10305"
     CDS             4463..6937
                     /locus_tag="EYO68_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08231.1"
                     /translation="MSSLIHQLNLFFEVLPERLHRWRWWVLLLYVILMSFLAAGIPRF
                     AFTWANEDMFAKDDPVQISQDKIRELFGGTVILGMAYRPVDGDLFSERSLGALQNIHK
                     VLDEEATNGADNPDNSFGLLSEVESLINTTYIEVEGDSIRFRDFIGENLPQSPEQSRL
                     LQGQALKEPEYPRVLFSDNMKYGMLVLRTNLNAIPLVDDELDLLEGFVENEIEEQDEQ
                     KPQFVKHGLEEYADFEKQIWNLLSEERFRKDLVFMHPSWGAFYQNDVWAVEYQRALVI
                     SLLLSLLLTYLLLGSLRAVIWPIIIFVSSIVGILGLAGWTGWPIDLSLYIAFGLVSVA
                     AIADVVHVMSGYLFFHQQGQHHREVMRSVFSKTALACLLTSITTAIGIMSLFFIKLRV
                     IQTMGLLGGIGVIFAFVLTIILLPVLLNWFPPQPSITAKKPKVTMILSFVQGLLQGVN
                     KLSFNYPKFVIFFFAASGILLILGILRIEIDTVFSEYYSPESPVRQTINLMDEQFLGV
                     SNMEILIETETEGAFRDPEVLLTLESVKQWLQTVYPELVTHNWTLNNQLKQTHRKLMD
                     NREEAYTIPEDPDLVSQLLVLIEGGDYEDLERMVSLDYSNARMSVSLKTIGSKKSVEL
                     LKVIQPEVERILVPLKEKYPGLKMTVTGGIGAWARIFDAISWSQIRSFGLAFMIISLI
                     MVGIFGSLKLGLVAVVPNTFPMLTVFGLMGWFGFKLDTTTLLTAPIIIGVAVDDTIHF
                     ITHYRLSVLRGASINEAIQSTLSEVGQAIVFTTLILMTIFVCFIMVSHVGVSRFSILA
                     VISIFSALIADLLLLPALFRVLQVRD"
     gene            complement(7003..7824)
                     /locus_tag="EYO68_10310"
     CDS             complement(7003..7824)
                     /locus_tag="EYO68_10310"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HIC08232.1"
                     /translation="MPEKLLILGGTREAYELAERLVSQFSPEQLTVISSLAGVTEHPR
                     QPAGEVRIGGFSDTTGTGGKSGLQNYLLQEKISLLVNATHPYAAQISENALAAATELQ
                     IPFLRMTRPPWVKQPGDQWIEVPDLAAAAEYLKTEFEISNSDLSGATVFLTTGNRGLE
                     LFEQCKRCNFVVRTVDVPKLNKSASVISAWSDATFLQERGPFTLENELNLFRQHAITL
                     LVTKNSGGDSTYAKIEAARKMGIPVLIVERPQLKPSDVCSTVAEVMNFVLRHSTK"
     gene            complement(7817..8929)
                     /locus_tag="EYO68_10315"
     CDS             complement(7817..8929)
                     /locus_tag="EYO68_10315"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HIC08233.1"
                     /translation="MIDKPSGALRRGWTTGACATAATKAALTSLITGDLSNSVSIILP
                     KGEQPEFALSHTELGTDFSTAAIIKDAGDDPDVTHGAEISVTVRNGIPGSGVVFKAGS
                     GVGTVTKAGLALEIGEPAINPVPRQMMCAVVSEIAETFNHSGDIVLTISVSGGEELAK
                     KTWNPRLGILGGISILGTTGIVIPYSCSAWIHSIHRGIDVARANGMQHVAGCTGSTSE
                     KLVQNHYRLPDEAMLDMGDFVGGLLNYFRKHPLPKLTIGGGFGKLTKLAQGHLDLHSG
                     RSQVDFLQLAEQLKRLGANEKLCEKTKTANTANEVLKLAQNAGLPLADRIAEQACIVV
                     RKALRKTETQINILVISRTSGAKLQGELVGEAENNA"
     gene            9048..9824
                     /gene="cobA"
                     /locus_tag="EYO68_10320"
     CDS             9048..9824
                     /gene="cobA"
                     /locus_tag="EYO68_10320"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIC08234.1"
                     /translation="MQSLPFDLPKFEPGWVWLAGAGPGDPGLLTLHALNALRQADVLV
                     YDALVGKGILELVSGDCELEYAGKRGGKPSAKQRDISLRLVELAEQGKRVLRLKGGDP
                     FVFGRGGEEALCLGKAEIPFRIIPGISAGIGGLAYAGIPLTHRDINSAVTFITGHGMA
                     GELPERHNWDAIAQGSPVIVMYMAMKYLGEIAVRLMRNGRSTEEPVAVITQASLPDQR
                     VLETKLGTCAVDVEKHGLKPPAMIVVGEVVSLREFLKWMD"
     gene            9875..10489
                     /locus_tag="EYO68_10325"
     CDS             9875..10489
                     /locus_tag="EYO68_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIC08235.1"
                     /translation="MYIPAHFKMNDTAEIRTFVMAHPFGALLTNGKQVPQVTHLPMQL
                     LTDDAGNDSINLHLSKANPHAKGLENRENAVAVFLGTNGYISPRWYAAKDNVPTWNYT
                     AVHAIGTLRKIENEEELMKLVDKLSIEHEAGAKSPWRADWSNSKIRKMVNAIIGFELK
                     VERWEGKEKVGQNRSAEDQASLKLNLENSGDPAYQFLAKQMKTS"
     gene            10503..11108
                     /locus_tag="EYO68_10330"
     CDS             10503..11108
                     /locus_tag="EYO68_10330"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIC08236.1"
                     /translation="MVKITKVYTRTGDTGQTGLVGGKRLPKDHPRIEAYGSVDELNSV
                     LGIVLSFLVQKDASKRRDKLELILEAIQQKLFDLGSELATLPGDEYVGQITLQAEDAE
                     WLEEIIDVMNDELQPLKSFILPGGTSLNAFLHLARTVCRRAERDILKLKQIDLVSPEI
                     IKYINRLSDFLFVAGRWVTETLGEKETLWQPGKSKPDWNWN"
     gene            11109..14852
                     /gene="cobN"
                     /locus_tag="EYO68_10335"
     CDS             11109..14852
                     /gene="cobN"
                     /locus_tag="EYO68_10335"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIC08237.1"
                     /translation="MHRIATKPGDLDSEKKLESVRQTPADILFISTADTELSGLAQVW
                     GKRFRKKARLTLRLMQANPLQHPAAAEHYADNVLCKAKLAIFRLHGGYGYFPHILDEI
                     LHIKSHGAKTRILVLPGTDEWDPELMNFNDYAEPLVRQMFSYFREGGIDNMELAAEAV
                     ELLLENKTGGFPEALKIPTFGWLKDKSGKRNQKSKIGRVWITFYRALQQTGDMAVVEA
                     LTEALNKQGLEVSGFYAYSLREIEAQEELLRKAEMEPPDAILTMQSFSIGTGPSGGSM
                     DEGSKTHISFLERLNCPVIQVPTSTENREAWLQNPRGFSASNAAMSVVLPETDGRLFS
                     TVVGFKQEQEAAPELEFRSKRLAPDAKQIEHVAELTANWVRLRRTANAEKRVAIILAN
                     YPNKDSRLGNGVGLDTPASVIAFLKDLDKRGYCISSFPGTETGATSVSRAESGATVEN
                     LEEKIPETGDELIRILQAGITNDAEMSYGKTPEQGISRERLFEMIGALQEVSQATLAK
                     QWTHEVADSVPVAGKRFGNIFIGIQPQRGFGLQTQAIYHDPALSPPPEYLAFYQWIRE
                     DFDAHAVIHFGKHGNLEWLPGRSVALGSDDFPQIALKTLPNLYPFIVNDPGEGAQAKR
                     RSSAVIVDHLTPPLTRAGLYEELDKAERLLEEHAHCETLFPERAHELEHEIEHLLEHA
                     EWSEELPEDDDPLNALSSHLCELKESQIRSGLHIFGQLPEGEKRIDFLLSLLRMPSVE
                     RPGLLQALLGKEPDFDLDTLSIRERDEIEEKARSWVKAEINLTLNSKNNSETKNSTSE
                     ISRWLHETLLPRFMRCAEETRSLALALEGRFVSPGPSGAPTRGRIDVLPTGRNFYSVD
                     PRVIPTQTAWRCGQALAEELIERYRSDHGEFPKTTALVIWGTSNMRTGGDDIAQALAL
                     WGCEPVWEPVSGRVVDFEIMPLSVLGRPRVDVVLRVSGMFRDSFGDVMRLLSTVPKRL
                     AELDEPQDMNPVRAAWLEDQERLQITGVSAENAKRLAELRVFSSGPGAYGTGLLPLID
                     AGNWETRGDLTEVFLKWGGHAYASDGTSSEEINLLRERLSSVEIVHQNQDNREHDILD
                     SDDYFQFQGGLQAAVTEIKGSTPATYHGDSSNPEKIKIRTLKEEFNRVFRSRVLNPKW
                     LESMREHGYKGAFEMAATVDYLFGYDATCDIVADYQYEEVAQKLLLDPEQQKFFREHN
                     PLALRDASQRLLEANEREMWENANPETLEALESAILEIQGEVE"
BASE COUNT         4042 a         3202 c         3763 g         4074 t
ORIGIN      
        1 ttttgtggga cgttggcgca ggttgcggtt cggtagcaat tgaatggatg cgtacaagcc
       61 ctcgctgtcg ggcagtggct attgaaaaat ccaagtctcg tttgaaactg attgaacaaa
      121 actctcaaga gttgggggtg ccaatgctgc agataatttc cggaagtgca ccggaagttt
      181 tggcggattt accggcgccg gacgcggttt tcattggcgg aggtctttcc tctggaaatt
      241 tgctgcagac ctgctggaat gcactcaaac ccggaggtcg cttagtcgcc aatgcggtta
      301 cgcttgaagg cgaacaaaaa ttgttgcagt ggcaaaatga aaatgccgga aagtccggtg
      361 ctagtggtga tttaacacga cttagtattt ctcgtgctga aaaaattgga aaattccagg
      421 gctggaaaga gatgcgttct gtaattcaac ttgctgttat taaaggttat taatttaaat
      481 ccaccacaga gtcacagaga acacagagat ggaattaaat aaaatttcag aacaaatttt
      541 tcggttcagc gatagaagtt cataaaatac ttggtcccgg actgctggaa tctgcatatg
      601 aagaatgttt gtgtagagag ttggagacaa ggaatatctc atttgaaaga caattaccgc
      661 tacctctgga atacaagggg aagccactgg attgtgggta taggctagac cttttggttt
      721 caaatacgat tgtagttgag cttaaagctg tctcattaat cgagcctatc catgaagcgc
      781 aattactcac ttatatgaaa ttaggagggt ggaagttggg attgctaatc aatttcaatg
      841 tccctctttt gaagaatgga attagaagaa gaattcttta aatactctgt gttctttgtg
      901 actctgtggt gaaaaataaa tcaggaaccc tttacggaat cggtgtgggg ccgggagacc
      961 cggaactgct aaccctcaaa gctcaccgcc tgatcaacga atgcgatgtt ctggcttatc
     1021 cggctccgga agttggtgac gggctggctt tgcaaattgt aagtccgttg ttgaccaatg
     1081 atccagattt gtttccactt cggttgccga ttgcagtctc tccttttccg gcacaacaag
     1141 cttacgatca agcagcaaaa atattggaaa ttccgctgga atcaggaaaa tcagtagccg
     1201 tgatttgcga aggcgaccca ttcttttatg gttcgtttat gtatttgttt gagaggcttg
     1261 caacaagttt caagactgaa attgttcccg gagtttcttc accgatggct tgtgcggcag
     1321 tattaaaaac accacttgtt tcacgcaacg aaattttaac tatattgcct ggtcctcttg
     1381 aagaaaaaga acttgagcag cgtttgttag acaccgattc tgcagcaatt atgaaagttg
     1441 gaagacacct gcctaaaatt cggagagtcc ttcggcactt gagactcgag cattgtgcgc
     1501 aatatgtgga acatgccacc atgcataatc agcaaatcat tccaattgaa aatctagaag
     1561 cacaaaaagt cccctatttc tcaatgattc tcgttcacaa aaatacgggt tactcaagca
     1621 atggtgttaa atgagtcagc aaattgcagt atttttattg ggaatcagtg cttttccggt
     1681 tgcaaaaaaa atagctgcag agcttggtgc agaactccat ggaaaggcag atcgtatttc
     1741 acaggtaagt tctgaaccag aagctgatgt tttttttact gacgcaatgg agcatctgtc
     1801 gactttgttt agcgaaggtg tagcaattgt cggtgtttgt gcttcagcag ttttgattcg
     1861 cggagtggca ggatgtttac aaaacaaact caacgagccc cctttgatag cagtggcaga
     1921 ggacggaagt gctgttgtac ctttgctggg aggacatcac ggagcaaatg atttagcgca
     1981 tcaaatagcg gatattttgg gaatatctcc ggccgtcact actgccggcg atttacgttt
     2041 tggaatagca ctggacgaac ctccggaagg ttttgtgttg agcaatccgg aagacgtgaa
     2101 gcatttcact gccgaactgt tgtcagggga atacgtttcg ttgtcttcag gagacaaacc
     2161 ggcagccccg gattacatgc cggggtttta caaatggttg caggatagcc ggttgccttt
     2221 ttcggagaat gcaaagctac ggatttcact ggccgcaaaa ccaatttccg gaaatgcaga
     2281 acatctggtt tttcaaatcg cacctgattt ttcggtaaag aacattgcgg ttggtgtggg
     2341 atgcgagcgc ggaacggatc cggaagaatt gattacgctg gtcctgaaca ccctacacga
     2401 aaatgatatt tctccggaaa gagtggcggt cgtagtttcg cttgatctca aagcagatga
     2461 accggcggtt catgctgtag caaaaaattt agaatgttcc gttcgttttt ttgacgcggc
     2521 tactttggaa gcactaactc cgaaattaaa gaatccttca gaaattgttt ttcaggaagt
     2581 tggatgtcac ggagtggcgg aaggtgcagc actcgcggct gttggcgatt ccggaatctt
     2641 gctggtaccg aaagtcaaat cctccggaat gtcctgtgca gggaggggca cttgcgctat
     2701 tgcagagtcg gctgagtttt tggatccgca aatgattgga cgggcgcagg gaactttgtt
     2761 cattgtaggt acaggtccgg gaacaccgca gtggcggcta ccggaaagtg aaaatatgct
     2821 ccggaaggca actgactggg ttggttatgg actctatctg gacttgattt ctgatttgca
     2881 caacggtcaa aaacagcacc gttttgattt gggccaggaa gaagtgcgtg ttcgtcatgc
     2941 tttgaaactg gcagcgcaag gcaaaacggt agctttgatt tcatccggag atcctgggat
     3001 ttatgcaatg tcttcgctgg tttttgaatt gcttgaaacc ggaaagtctg gtgcctccgc
     3061 aaagtcctgt gccaaggata attctgtcgc cgaatggcaa cggattcatg ttgaagccac
     3121 tcccggaatt tctgctttgc aggcagcaag cgctcggatt ggtgctccgc tcggacatga
     3181 tttctgcaca atttcgctct cagatttact cacgccgtgg gaaacgattg aacgccgaat
     3241 tcgtgccgct gcagaaggag attttgtgat tgctttttat aatccggttt cacgccgccg
     3301 cactacgcaa ctggtccggg cacaggagat tttgttgaaa catcgacctg cggaaacacc
     3361 ggtcattttt gcccggaacc ttggtcgtga tgaagaaaaa gttaaggttg tttcgttgga
     3421 aaacctggat ccagcacagg ttgatatgct gacagtggtt ttggtaggat caagtcaaac
     3481 ccgcctatta gagttgccaa acggtgaagt caagatttat actccacgtg gatatgatca
     3541 aaaatctaaa aactaaagac agtaaatcat aaatcgaatg acagtacact ttattggagc
     3601 aggaccggga gcgccggatt taatcacaat tcgcggacga gacttaatcg caaaatgtcc
     3661 ggtatgtctt tatgcaggct cgctggttcc ggaagaagtg gtagcttttg ctccggcgag
     3721 tgcgttggtg ctggacacgg cgccgctgaa tttggaagaa atcattacgg aaatacaaac
     3781 agcacatgag gccggaaaag atgtggcacg tgtgcattcc ggcgacccgt caatttatgg
     3841 agccactgcc gaacaaatgc ggcggctgga cgagttggat attccgtatg atgtgacacc
     3901 cggtgtacct gcgtttgcgg ctgcggctgc cgaattaaaa caggaattga ccttgccgga
     3961 agtaagtcaa accatcattg ccacgcgcac caccatgcac gcttcagcca tgccggaagg
     4021 cgaagaactg gcaaaactag ccgcaatagg ggcaaccctg gtcattcact tatctgtgcg
     4081 caacctcaag tttgtgcagg atgagctgat tccacattac ggaaaggatt gtccggttgt
     4141 ggtggcctat cgggtgacct ggccgaatca acagttcatt cacggaacat tggccaacat
     4201 tcgagaaaaa cttcgtaaat ccaaaatcag ccgcaccgca ctgattttag tcggtcgagt
     4261 ttttgatgca aaaaattttc gtgacagtgc tctttatgat ccgcagcatg tacacgtgtt
     4321 aaggccaaag aaaaaatcag taaaaaaatg atggtaatta ttggtgttca cttcggtttg
     4381 ttttaaattt ttctttctga gactttgtaa tcaaactttc ctcaactcac ttgttttcat
     4441 ctctcttcca aaaacttgaa gcatgagttc cctgattcac caactcaatc ttttttttga
     4501 agttctgccg gaacggctgc atcgctggcg ctggtgggtg ttgctgttgt acgtgatcct
     4561 tatgtccttt ctggcggctg ggattccgcg attcgctttc acctgggcta atgaggatat
     4621 gtttgctaag gatgatcctg ttcagatttc tcaggataaa ataagggagc tctttggagg
     4681 tacagttatt cttggcatgg cctacagacc cgtcgacgga gatctttttt ctgaaagaag
     4741 tctgggtgcc ctgcagaaca tccataaggt gcttgatgaa gaagctacca acggcgcgga
     4801 caatccggat aattcatttg gccttctgtc cgaagtggaa tccctgatca ataccaccta
     4861 catcgaagtt gaaggagaca gtatccgttt ccgggatttc attggtgaaa acctgcctca
     4921 aagtccggaa cagagcaggt tactgcaggg gcaggccctc aaagaacctg agtatccgcg
     4981 tgttcttttt tcggataaca tgaagtatgg aatgctggtg ctgcggacta acttaaatgc
     5041 cattccgctt gtagatgatg agcttgacct gctggaagga tttgtcgaaa acgagattga
     5101 agaacaagac gaacagaagc cacagtttgt aaagcatggt ctggaggaat atgcagattt
     5161 tgaaaaacag atctggaacc tcctgagtga agaaaggttc cgcaaggatc tcgtgttcat
     5221 gcatcccagc tggggtgcat tctatcagaa cgacgtctgg gcagttgaat atcagcgggc
     5281 actggtcatc tcactcctct taagtcttct gctgacatat ctgttgctgg gttcactacg
     5341 agcggtgatc tggccgatca ttatctttgt gtcaagcatt gttggtattc taggactggc
     5401 aggctggaca ggatggccca tcgatctttc actttacatt gcattcggac tggtcagcgt
     5461 tgccgcaatt gccgacgtgg tacacgtaat gtccggctac ctgttttttc atcagcaggg
     5521 acagcatcac agagaagtaa tgcgttctgt tttcagcaaa actgcgctgg cctgcctgct
     5581 gaccagcata actactgcca tcggtatcat gtctctcttt ttcatcaagc tgcgggtgat
     5641 acagacgatg ggactgctgg gaggtatcgg ggtgatcttt gctttcgtgc tgacaattat
     5701 tttacttcct gtcctgttga actggttccc tccgcaacct tccataaccg ccaaaaaacc
     5761 taaagtcacc atgattctga gttttgtaca gggcctgctg cagggggtta ataaactgag
     5821 ttttaattat cctaaattcg tgattttctt ttttgctgcc agcggaattt tgctgatatt
     5881 gggtatctta cgaattgaaa ttgatacagt tttttctgaa tattattcgc ctgaatcacc
     5941 ggttcgccaa actatcaatc tgatggatga gcagtttctt ggggttagta acatggaaat
     6001 cctgattgaa actgaaactg aaggagcttt ccgggatcca gaagtacttc ttacacttga
     6061 atcagttaaa caatggcttc agacagttta tccggaactg gtgactcata actggacgct
     6121 taacaaccag ctcaagcaga ctcatcgaaa gctgatggat aacagagagg aggcctatac
     6181 gattcccgag gatccggatc ttgtgtcaca attgctggtg cttattgaag gaggggatta
     6241 tgaagatctg gaacgcatgg tttcactgga ttacagcaat gcgagaatga gtgtttcact
     6301 gaaaaccatt ggatcgaaaa aatcggttga acttctgaaa gttatccagc ccgaggtcga
     6361 acggattctc gttcctctaa aggaaaaata tcctgggctt aagatgacag taactggtgg
     6421 tatcggtgcc tgggcccgta tttttgacgc aatcagctgg tctcagatac gcagttttgg
     6481 attggcattt atgatcattt cgttgattat ggttggcatt ttcggatcac tgaaactcgg
     6541 actggtggca gttgtgccta atacattccc gatgttgacc gtatttggac tgatgggctg
     6601 gtttggtttt aaattggaca ctaccactct gttgacagca ccgattatca taggagttgc
     6661 agtggacgat actattcatt ttatcacgca ctaccgacta tcagtgctcc gcggagcaag
     6721 cattaacgaa gcaatccaat ccactctcag cgaagtcgga caggcgattg ttttcaccac
     6781 gctgatacta atgaccattt tcgtttgctt catcatggtc agtcacgttg gagtttcacg
     6841 tttcagcatt cttgctgtta tctccatttt ttcagccctg attgccgatt tattgctttt
     6901 gccggcacta tttcgagttt tacaagtacg ggattagatt tttgaattca cattttgatc
     6961 tgatgaatta actgtaaagt ccaagcttaa aacctgattt tttcattttg tgctgtgtct
     7021 aagtacaaag ttcatcacct cagcaactgt actgcaaaca tctgatggtt taagctgcgg
     7081 tctttccaca atcaaaacag gaattcccat tttccttgca gcttcaattt ttgcgtaggt
     7141 tgaatcaccg ccgctgttct tggtgacaag cagggtgatg gcatgttggc gaaacaggtt
     7201 gagttcgttt tccaaagtga atggtccccg ctcctgcaaa aaagtcgcat ccgaccaagc
     7261 ggaaatcacg gaggcggatt tattgagttt cggaacatcc actgtacgca ccacaaaatt
     7321 acatctttta cattgctcaa aaagctctaa acctcgattc ccagtcgtca aaaaaactgt
     7381 ggcccctgat aaatcggagt tcgaaatttc aaattcggtt tttaaatatt ctgcggcggc
     7441 tgccaaatcc ggaacctcga tccactgatc tccgggttgt ttcacccagg gtgggcgggt
     7501 catgcgcaaa aagggtattt ggagttccgt tgccgccgcg agtgcatttt cactgatttg
     7561 tgcggcatag ggatgggtgg cattgacaag taacgaaatt ttttcctgca gcaaataatt
     7621 ttgcagtcca gacttaccgc ccgtaccagt tgtatcggaa aagcctccga tccgtacttc
     7681 tcctgcaggt tgtctcggat gttcggtgac tccggccaga gaactgatga cagtcaactg
     7741 ttccggagaa aattgcgaaa ccaggcgctc agccagttcg taggcttcac gtgtcccacc
     7801 caggatcagg agtttttcag gcattgtttt cggcctctcc aacgagttcc ccttggagct
     7861 ttgcaccgga tgttctggaa atcactagaa tattgatttg agtttctgtt ttacgtaacg
     7921 cttttcgaac aacaatacag gcttgttctg cgattctatc cgcaagcggc agtcctgcgt
     7981 tttgtgccag tttaaggact tcattcgcag tgttggcagt tttggttttc tcgcatagtt
     8041 tttcatttgc accaagtctt ttcagttgtt ctgccaactg caaaaaatcc acctgactgc
     8101 gtccggagtg taaatcaaga tgaccttgtg ccaacttggt gagtttccca aatccacctc
     8161 cgatagtgag tttcggcaaa ggatgttttc gaaaataatt aagtaagccg cccacaaaat
     8221 ctcccatatc aagcatggct tcgtccggaa gtctgtaatg gttttgcact aatttttcgc
     8281 tggtggaacc ggtacatccg gcgacgtgct gcattccgtt tgcccgagcg acatcaattc
     8341 cacgatgaat agaatgaatc caggcggaac aggaataagg aatgacaatg ccggttgtgc
     8401 ccaaaatcga aattcctcct aaaatgccga gtcgtgggtt ccacgttttt ttagctaatt
     8461 cttcaccacc ggaaacggaa atcgtcagta caatgtctcc ggaatgatta aatgtttcag
     8521 caatttcgga gacaacagcg cacatcattt gccggggaac tggattgatt gccggttctc
     8581 cgatttccag cgcaagtcct gctttcgtca ctgtacccac tccggaaccg gccttgaata
     8641 ctactcctga tcccggaatt ccatttctta cagtaacgct gatttcagca ccatgtgtca
     8701 cgtccgggtc gtcaccagca tctttgataa tagcagcagt tgaaaaatct gttccaagtt
     8761 ctgtgtggct cagtgcaaat tccggctgct cgcctttggg taaaatgatg ctgacagaat
     8821 tcgacaaatc accggttatc agcgaagtta aagctgcttt tgttgcagca gtagcacagg
     8881 ctcctgtggt ccagccgcgc cggagcgctc cggaaggttt atcaatcatg gcgcaataga
     8941 atcttagctg taaaaatctt gtttatcctg ttattctgtc acaagcgaaa aagatacaat
     9001 tttatgactg taacgcattt tgaaattttt ttcagagcaa aaaatttatg caatcacttc
     9061 cttttgattt gccaaagttt gaaccgggat gggtttggtt agcaggagca ggtcccggag
     9121 atccaggatt gctcaccctt catgctttaa atgcacttcg ccaggccgat gtgctggttt
     9181 atgatgccct ggttgggaaa ggaattctag agttggtttc aggagattgc gagcttgaat
     9241 acgccggcaa acgtggaggc aagccttcgg caaaacaaag agatatttct ttacgtctgg
     9301 tggaattggc agagcagggc aaacgtgttt tacgactcaa ggggggtgac ccgtttgtat
     9361 ttggcagagg cggcgaggaa gcattgtgtc tgggaaaagc tgaaattcct ttccggatta
     9421 ttcccggaat ttcggcagga atcggaggtc tagcctatgc tggaattcct cttacgcatc
     9481 gtgatattaa ttcggcagtc acgttcatca ctggacatgg aatggcaggt gaacttcctg
     9541 aaaggcacaa ttgggatgcg atagcacagg gatcaccggt gatcgtgatg tatatggcga
     9601 tgaaatatct tggtgagatt gcagttcgcc tgatgcggaa cggtcgttct acagaagaac
     9661 cggttgcagt aataactcag gccagtttac cagatcagcg tgttttagaa accaagctcg
     9721 gaacttgtgc ggtagatgtt gaaaaacacg gacttaaacc gcctgctatg attgtggttg
     9781 gtgaagtggt gtctttacgt gaatttttga aatggatgga ttagcttgag gcagttaatt
     9841 tcgaaaaaca acaaaaaact ataaaaaggg aaatatgtac ataccagcac acttcaagat
     9901 gaacgacacg gctgaaattc gaacttttgt gatggcacat ccgtttgggg ctctactgac
     9961 aaatggcaaa caagtcccgc aagtgactca cttaccgatg cagcttctga cagatgatgc
    10021 ggggaatgac tcgatcaatc ttcatttgtc taaggcaaac ccacatgcta agggactcga
    10081 gaacagagaa aacgcggttg cagttttttt aggaacaaac ggttacattt cgcctcgctg
    10141 gtatgccgca aaagataatg ttccaacctg gaattacact gcagtgcatg caattggaac
    10201 tttgcggaaa attgaaaacg aggaagaatt aatgaaactg gtggataaat tgtccataga
    10261 acatgaagcg ggtgcaaaat ctccatggcg ggcagactgg tctaattcaa aaattcgaaa
    10321 aatggtaaat gcgattattg gttttgagtt gaaggttgaa agatgggagg gaaaagaaaa
    10381 agttggtcaa aacagatcgg ctgaagacca ggctagtctg aaactaaatt tggaaaattc
    10441 aggagaccct gcttatcaat ttctcgcaaa acaaatgaaa acaagttagc tcacataaaa
    10501 tcatggtaaa aattacaaaa gtttatactc gtaccggaga tacaggacag accggactcg
    10561 tgggaggaaa gcggcttccc aaagatcatc cacgtattga agcttatgga agcgtcgatg
    10621 aattgaacag tgtcttggga attgtattaa gttttcttgt gcaaaaggat gcatcaaaac
    10681 gaagggacaa actggaactg attttggaag caattcagca aaaactcttt gacttgggtt
    10741 ccgaattggc aactcttcca ggtgatgaat atgtggggca aatcacttta caggctgaag
    10801 acgcggaatg gcttgaggaa ataattgatg tgatgaacga tgaacttcaa ccactgaaaa
    10861 gttttatcct gcccggcgga acgtcgctca atgctttttt gcaccttgca cgtacagttt
    10921 gtagaagggc cgagcgagat attctcaaac tcaagcaaat tgatttggtg agtccagaaa
    10981 ttatcaaata cataaaccgt ctttccgatt tcctttttgt cgccgggcgc tgggtcacag
    11041 aaacccttgg cgaaaaagaa accttgtggc agccgggaaa aagtaagcct gattggaatt
    11101 ggaattaaat gcacagaatt gccaccaaac cgggagactt ggactccgaa aaaaaacttg
    11161 aatcagtccg tcaaacacca gccgatattt tattcataag tactgctgat accgagcttt
    11221 ccggactggc ccaggtttgg ggcaaacgat tccggaagaa agcgagactt acactgcgtt
    11281 tgatgcaggc caatccgctt caacatcctg cggctgcaga gcattatgcg gataatgttt
    11341 tgtgcaaagc caagttggca atatttcgtt tgcacggtgg atacggctat ttcccgcata
    11401 tactggacga aatccttcac atcaagagtc acggtgcaaa aacacggatt ctagtgctac
    11461 ccggaactga tgaatgggat ccggagttga tgaatttcaa tgattacgca gaaccactgg
    11521 tgcgtcaaat gttttcatat ttccgcgaag gcggaatcga caacatggaa cttgcagctg
    11581 aggcggtgga attgctactg gaaaataaaa ctggggggtt tccggaagct ttgaaaattc
    11641 caacttttgg gtggctaaaa gataaatcag gaaaaagaaa tcaaaaatca aaaattggtc
    11701 gggtttggat taccttttat cgtgctttgc agcaaacggg tgacatggcc gtggttgagg
    11761 ctttgactga ggccttaaac aaacagggct tggaagtatc gggattttat gcctattcgt
    11821 tgcgtgaaat tgaagcgcag gaggagttgc tgcgaaaagc tgaaatggag cctccggacg
    11881 cgattttgac catgcagagt ttttcgatcg gcaccggacc ttccggcgga agcatggatg
    11941 aagggagtaa gactcacatt tcttttttgg aaagactgaa ttgtccagta attcaggtac
    12001 cgacttccac tgagaatcga gaagcgtggc tgcaaaatcc acgcggtttt tctgcctcta
    12061 atgcagcaat gtctgttgta cttccggaaa cagacgggcg gcttttcagt actgtagtcg
    12121 gttttaaaca ggaacaggag gcagcacctg aattggagtt tcgttcgaaa cgtttggctc
    12181 cggatgcaaa acaaattgag catgtagctg agcttactgc aaattgggta cgtttgcgcc
    12241 gaacagctaa tgcggaaaaa cgagtggcaa ttattctggc aaattatcca aacaaagaca
    12301 gccgccttgg aaacggagtt ggacttgata caccggccag cgtaattgct tttttgaaag
    12361 atttagataa acgcggatac tgcatttcat ctttccccgg aacggagacc ggtgctacca
    12421 gtgtttccag agcggagtct ggagctaccg tagaaaattt ggaagaaaaa atcccagaaa
    12481 ccggtgacga actgatccgc attctgcagg caggaataac caacgatgcg gaaatgagtt
    12541 atggaaaaac accggaacaa ggcatttccc gcgaacggtt atttgaaatg atcggtgcgt
    12601 tgcaggaagt aagtcaagca actttggcta aacaatggac gcatgaagtt gcggattctg
    12661 ttcctgttgc cggaaaacgt tttggaaaca ttttcatcgg catccagccg cagcggggat
    12721 ttggtttgca gactcaggcg atttatcacg atcccgcttt gtctcctcca ccggaatacc
    12781 tggcgtttta tcagtggatt cgtgaggatt ttgacgcaca tgccgttatc cattttggaa
    12841 aacacggtaa tttggaatgg ctgcccggaa ggagcgtagc cttggggtca gatgattttc
    12901 cgcaaattgc attgaaaact ttaccaaatt tgtacccttt catagtcaac gatcctggcg
    12961 aaggcgcaca ggctaaacgc cgttcgtctg ccgtaattgt cgatcacctc accccgcctt
    13021 tgacccgcgc cgggctttac gaagaattgg acaaagccga acgtttgctt gaagaacacg
    13081 cccattgtga aacgctgttt ccggaacgcg cccacgaact cgaacacgaa attgagcatc
    13141 tgctggaaca cgcggagtgg agtgaggaac ttccggagga cgatgatccc ttgaatgcgc
    13201 tcagcagcca cctttgtgag ttgaaagaaa gtcaaattcg ttccggtttg cacattttcg
    13261 gacaacttcc ggaaggcgaa aaacgaattg attttctgct gagtctgctg cggatgccct
    13321 ctgtggaacg tccgggtttg ctgcaggcac ttttgggaaa agaaccggat tttgaccttg
    13381 acacgctttc gattcgggaa cgggatgaaa ttgaagaaaa agcaagaagt tgggttaaag
    13441 ctgaaatcaa cctgacactt aattcaaaaa ataattcaga aacaaaaaat tccacatccg
    13501 aaattagccg ctggctgcac gaaactttat tgccgcgttt tatgcgttgt gcagaggaaa
    13561 cccgcagttt ggctttggct ttggagggac gctttgtcag tccgggaccg tccggcgcac
    13621 caacgagagg gcggattgac gttttgccga ccggacgcaa tttttattca gtcgatccgc
    13681 gtgtgattcc aacgcagact gcctggcgct gcggacaggc tttggccgaa gaattgattg
    13741 aacgctaccg ttcagatcac ggcgaattcc cgaaaactac agcgcttgtg atttggggaa
    13801 catcgaatat gcgtaccggc ggcgatgaca tcgcccaggc tttggcgctt tggggttgcg
    13861 aaccggtttg ggaaccggtt tcagggcgtg tagttgattt tgaaattatg cctctttcag
    13921 ttttgggtcg accgcgggtg gatgtcgttt tgcgggtttc cggaatgttc cgggactcct
    13981 ttggtgacgt gatgcgtttg ctttcgactg taccaaaacg tttggcagaa cttgacgaac
    14041 cacaggatat gaatccggtg cgggcagcat ggctggaaga tcaagagcgt ttgcaaatta
    14101 ccggagtttc cgcggaaaac gctaaacggc tggcggaatt acgggttttt agttctggac
    14161 ctggagcata cggaaccgga cttttaccat tgattgacgc aggaaactgg gaaacccgtg
    14221 gcgatttaac tgaagtattt ttgaaatggg gcggccatgc ttatgcttcc gatggtacga
    14281 gttctgagga aattaatttg ttgcgtgagc ggctgagctc tgtggaaatc gtgcatcaaa
    14341 atcaggataa ccgtgaacac gatattctgg attcggatga ttattttcag tttcagggag
    14401 gactacaggc ggccgtcaca gaaataaaag gttccactcc cgcaacttat cacggtgatt
    14461 cgagcaatcc tgaaaaaata aaaatccgca cactcaagga agaattcaac cgtgttttcc
    14521 ggagccgtgt tctcaatcca aaatggctgg aatcaatgcg cgaacacgga tacaaaggtg
    14581 catttgaaat ggcggccaca gtcgattatc tgtttggcta cgatgcaacc tgcgatattg
    14641 ttgcagatta tcaatacgaa gaagtcgccc agaaacttct gctcgatccg gaacagcaaa
    14701 aattctttcg ggaacacaac cccctcgctt tgagagatgc ctcccaacgt ctgcttgaag
    14761 ctaacgaacg ggaaatgtgg gaaaacgcaa atccggaaac tctggaggca ctcgaatctg
    14821 caatactgga aattcagggg gaggtggaat aaagaaataa aacggataat ttgctcgttg
    14881 gagttgcgac aggtaaaccc aaatcatcct tctttaagcc tttatgcctt gcgtggtaca
    14941 cttaatttgc tccattatgt tcccatttcc tgcaatacag aattactctg gtattgggga
    15001 tcaaagaaaa acagattttg caggtgaatg tgagtagtca ccatgcagtc aattgaatac
    15061 agtttcttaa caataaagtc a
//
LOCUS       DTVD01000281            4045 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8057_length_4045_cov_354.151880, whole genome
            shotgun sequence.
ACCESSION   DTVD01000281 DTVD01000000
VERSION     DTVD01000281.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_4045_cov_354.151880"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="EYO68_10340"
     CDS             complement(<1..566)
                     /locus_tag="EYO68_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08238.1"
                     /translation="MDSQEGFGSLIPENIKKKLHNGGVQFSELRQAGKTPENLIETIY
                     RFWRGGSIRVERNWNGILDEAEIVQYSNSACLLPDDRIYLNTSTFRNLWKLATWNVNS
                     IRTRLPLILEWLEEHNPDVVCLQETKVEDQLFPVLELRQSGYECAFYGQKSYNGVAIL
                     SKHPIEDVKTGFRNGYDQDNARLIAGTVS"
     gene            845..1159
                     /locus_tag="EYO68_10345"
     CDS             845..1159
                     /locus_tag="EYO68_10345"
                     /inference="COORDINATES: protein motif:HMM:PF01052.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08239.1"
                     /translation="MSSKKQKRENPKKETGPQIQKLMEMPVTARLVLGECNLEIEEIL
                     RLGQGSMLVLDTNVKANLKLYINDEEIAKAKSVTIGDNLGAKITEISSTEKRLKDLTD
                     LE"
     gene            1218..2177
                     /locus_tag="EYO68_10350"
     CDS             1218..2177
                     /locus_tag="EYO68_10350"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIC08240.1"
                     /translation="MTTPSIRLLRESFPEAQFTFLTEPPSDQVLRENPYLDEILLFRK
                     TETLRETLRFFRTIRHHQFDTVIDFFGNPRSALITRFSGAPIRIGFDFRGRSLAYTHP
                     VKISTETTYAAQDKAQLLKPLGISAEDFGLDFFTAEKDQNYADDLFEKMGIKNKDFVV
                     SLSPVSRQPYKVWPAERFALIADWLIKTYNAKVLFLFGPGELHFIDSVRKAMTQTALP
                     DYAVPTLTETLAILNKVDLHLGNDNGPRHFAVAGGTPTLTIFGRPWAANWTPPQQTLH
                     RTLEYDPGCKNKCVYPKCNLECLDGVTVEAVQNELENVMKNIR"
     gene            2196..2804
                     /locus_tag="EYO68_10355"
     CDS             2196..2804
                     /locus_tag="EYO68_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HIC08241.1"
                     /translation="MFDTEKMLEQQLKEVEQWHEELPQESSSESVDFPALVSTQHQVN
                     FELWHQEDLARDPEVSDTKIAAVKRAIDVLNQRRNDLIEQLDQFLVNVMGSKKVKNSA
                     DLELNSETPGSIIDRLSINALKIFHMDEEIQREDAAESHRIKCSGKLSVLQEQRQDLG
                     QCLVKLLADLSTGKKRLKVYQQLKMYNDESLNPVLYQKVESK"
     gene            complement(3254..3454)
                     /locus_tag="EYO68_10360"
     CDS             complement(3254..3454)
                     /locus_tag="EYO68_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08242.1"
                     /translation="MARGGFREGAGRKKEYNEPVKKILLGLPESVLKQLDSFASEKNL
                     SRPKAVAILVQQAMQQGGQIAV"
BASE COUNT         1206 a          782 c          874 g         1183 t
ORIGIN      
        1 gaaacggttc ccgcaatcag gcgggcattg tcttgatcgt agccgttgcg aaaaccagtc
       61 ttgacatcct caattggatg tttagataaa atggctacac cgttatacga tttttgtccg
      121 taaaaggcac attcgtatcc ggactgccga agttccaaaa ccgggaacaa ttgatcttca
      181 acttttgttt cctggaggca gacgacatcc ggattgtgct cctcaagcca ttctaaaatc
      241 agtggcagac gtgtccggat agaattaaca ttccaggttg caagcttcca caaattccgg
      301 aaggttgagg tattaagata aatcctgtca tccggcagca aacaggcact gttgctgtat
      361 tgaactattt ccgcttcatc cagaattccg ttccagtttc gctcaacacg gatgcttccg
      421 ccacgccaaa aacggtatat tgtctcaatt aaattttcag gtgttttgcc ggcttgccta
      481 agttctgaaa actgaacacc gccattgtgg agttttttct tgatgttttc aggaatcagc
      541 gatccgaaac cctcctgaga atccattact cctgagctag agattgaagt agaattgcgt
      601 tctcctgaat ttgcagacgg cgttttctca tgtttttctt gtttggacgg ctgcttgcgt
      661 gtctttttgt tgcagttttt tgtttaggtg acattttgta ttcctgttat ttgagattaa
      721 atcatcttgt tctgattgaa aataatcggt atggtttaag tcttgtacgt ttaaaaatag
      781 caaaataatt ttgataatgc gagctaaata taaatcgaga aaatcttcca tttaaggaaa
      841 tgagatgagc agcaaaaaac aaaaaagaga aaatcccaaa aaagagactg gaccgcaaat
      901 tcagaaatta atggaaatgc cagttactgc tcgactcgtt ttaggcgaat gcaatctgga
      961 aattgaagaa atactcaggc ttggacaagg ttccatgctg gtgctggaca caaacgtgaa
     1021 ggcaaatttg aagctttata tcaatgatga agaaattgca aaagcaaaat ctgtaacaat
     1081 aggtgataat cttggtgcaa aaataacaga gatttcttca acagaaaaac gactcaaaga
     1141 tctgaccgat ctggaataaa ggtgatgctt cagtgaaatt tcttttgatc caattacgaa
     1201 ggattggtga tgtattaatg acaacccctt caatcaggct gcttcgtgag tcttttcctg
     1261 aggcacagtt tacatttttg acagagcctc cctctgatca ggttttgagg gagaatcctt
     1321 atttggatga aattctgctt ttccgaaaga cagaaactct ccgagaaaca ctaagatttt
     1381 tccggactat ccgtcatcat cagtttgata cagtgattga ttttttcgga aatccaagga
     1441 gtgccttaat tacccgtttt tcaggagcac cgatccggat tggttttgat tttcgcggaa
     1501 ggagcttggc ctatacccat ccagtcaaga tttcaacaga gacaacttat gctgctcagg
     1561 ataaggctca actgctgaaa ccgcttggaa tttctgcaga agattttgga ctggattttt
     1621 ttacagcaga aaaagatcaa aattatgcag atgatctttt tgagaaaatg ggtataaaaa
     1681 acaaagattt tgtggtttct ttgtctccgg tttcacgtca accatacaaa gtttggcctg
     1741 cagaaagatt tgctctgatt gccgactggc tgataaaaac ctacaatgca aaagtattgt
     1801 ttctgtttgg tccgggagaa ttgcatttta ttgattcggt tagaaaagcg atgactcaga
     1861 cggctcttcc ggactatgct gttccaacgc ttactgagac tcttgccatt ttgaacaaag
     1921 tagatttgca tcttggcaat gacaatggtc caagacactt tgctgttgca ggcggaacgc
     1981 caacgttgac aattttcggc agaccatggg ccgcaaactg gactccacct caacaaacac
     2041 tgcaccgcac tctggagtat gatccaggct gcaaaaataa atgcgtctat ccaaaatgca
     2101 atttggagtg tcttgacggt gtaactgttg aagcagttca gaatgaattg gagaatgtga
     2161 tgaaaaatat cagataaagg ggttgaatgg ttttcatgtt tgacacagag aaaatgctgg
     2221 aacagcagct gaaagaggtt gagcagtggc acgaagaact gcctcaagaa agttcttctg
     2281 aatcagttga ttttccggct ttggtctcaa cacagcacca ggtcaatttt gagttatggc
     2341 accaggaaga tctagctcgt gatccggagg tttcggatac taaaattgct gcagtcaaac
     2401 gcgcgattga tgttttgaat cagcgccgga atgacctgat cgaacagctg gaccagtttc
     2461 tggtgaatgt catgggcagt aaaaaagtga aaaacagcgc tgatttggaa ctgaacagtg
     2521 aaactccagg cagcataatt gaccgccttt ccatcaatgc acttaaaata tttcatatgg
     2581 atgaagaaat tcagcgtgag gatgctgcag agagtcatcg aataaaatgt tccggtaaac
     2641 tctctgttct gcaggaacag cgtcaagatt tggggcaatg tctcgttaaa ctgcttgctg
     2701 atttaagtac aggaaaaaag cggctgaaag tttatcagca attgaaaatg tataacgatg
     2761 aaagtttaaa tcccgtgctt tatcagaaag tcgaatcaaa gtgaacaggc actaaaatgg
     2821 gtccaaaggg aatttttctt gctttcataa ttgtctgtgc aatctattta tggaggaagt
     2881 accgcaaaaa agagccaacc aaatcatcca gatgggtgaa ttgggtgctt ggaggtattg
     2941 cggtttatta tggaatatct gccgtaatta tgtttttaga caaaggctaa gaaggtagat
     3001 caggaacaga ttagccggca acgctggatt tttactcaat tctgaaagtg gccggatgaa
     3061 tgattgatta agttgtggaa taaaaatggg tcttgtattg attggaatgc tggttgcctc
     3121 tatggtttta ttggggctgt ggcagaggcg cagagcaaaa aagcgtttcc tggaagcaaa
     3181 gcgcaaactc agggaactta actcaatgcc tgaatcagac actaattagt gatagttttg
     3241 taggggatga gaattagaca gctatttgac cgccttgctg catggcctgt tgtacaagaa
     3301 ttgccacagc tttaggccgc gagaggttct tttcagaggc aaagctgtcc aactgcttta
     3361 aaacggactc aggaagtcct aatagaattt ttttgacagg ctcgttatat tccttttttc
     3421 ttcctgcccc ctcccggaag cctcctcttg ccataataac ctacttcaac tcgttggatt
     3481 taaaaaactc aagaaattac ttattttgcc gatactatta gtacatcttt caagatgtat
     3541 gcaagagttt catgaaacag gtttcaaaac tgttttaatt actgatttta actaactaaa
     3601 tccgggagaa ccagatgagt acaaacaaaa aaattcttta tccttttcgt caaatagcca
     3661 ttcgcacgcg ctgtttgcac cgcttaaatc ctcaggaact catgatccgc agaggacaat
     3721 gaattccagg attaacggga ggaaatgtcc tcccgtagtc cctgtcattt tcttcataca
     3781 gtcctttttc ctaccctcct ttttgttatt taatcttcgt attctttgct aaaaagccaa
     3841 ctttgttcag gatcagtccg taaattttgc ctcccccgca aaaatgtgga atgatagatt
     3901 gtgacaattg taaacaaatc ggacctgaaa aaaattcggc ataatgtaca aaattctgat
     3961 taccggttca atccaccaaa ttggtttgga aatcctgcgt aaagaaaaag atattgaaat
     4021 ccagtatgct ccggatcttg ctttt
//
LOCUS       DTVD01000282            4038 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8084_length_4038_cov_146.374843, whole genome
            shotgun sequence.
ACCESSION   DTVD01000282 DTVD01000000
VERSION     DTVD01000282.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_4038_cov_146.374843"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="EYO68_10365"
     CDS             complement(<1..1019)
                     /locus_tag="EYO68_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HIC08243.1"
                     /translation="MDLTLLAGIFFCLTQSAVFSGLTLGLFGLSRLKLEIEVESGNQA
                     AVEVLELRKDANLLLTTLLWGNVSINVLLTLLTDSVLAGLWAFFFSSFCITLFGEILP
                     QAYFSRNALRMGILLTPVIKFYRFILYPVSKPSAMLLDMWLGKEGVLYFKEEDIRIML
                     QKHMESRESDISRLEGTGALNFLSIDDLPVGDEGEVVDPVSIIPLETEFDLPVFPAFQ
                     RTPDDPFLLQVQLSGKKWVILTSVDGKPRLVLNADLFLRDALFGVDPFRPYSYCHRPI
                     VVSDAATPLGEVIQKLRVHPEHSEDDVIDNDLILCWDTEKRIITGADLLGRLLRGIAG
                     RRPQFV"
     gene            1494..2516
                     /locus_tag="EYO68_10370"
     CDS             1494..2516
                     /locus_tag="EYO68_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08244.1"
                     /translation="MADLDEVRSLTETELADNKKIQNDYNRKQELLKKIAVEDVAEKK
                     ELLKEKGVVEKAKKELSELTEKILKKRRKKLLKAEVEKIAESLKEGVTVKEIVELLEK
                     VASDEISVEEATVILKKKTTLSDEGIKKLLAKTKAIQKETEELAEKLATASADEVAEI
                     LREAGGVSEKDILALVEKKKEVDAIESSFLEKTMAKLYTRLIRDRELGIEEAFCINIE
                     GILDHLLVEPSYFDTDKYSIVEYIDQIESSFRLLEPILEEYPEIIVRLEGNADERGTV
                     EYNKALSDRRWETPSKVLLALYFDEDRTAGVGRSEQCPLPRAGGISTRDWWSSNRRTD
                     YIFKLK"
     gene            2526..3266
                     /locus_tag="EYO68_10375"
     CDS             2526..3266
                     /locus_tag="EYO68_10375"
                     /inference="COORDINATES: protein motif:HMM:PF01618.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08245.1"
                     /translation="MEFNDFVNAVLHGGTITLSIFILLVVLSVVTWGIIIGKFVQLRK
                     EDQNSGKFVKVFNKAKSLKSFLSTNHKRAPVDYDLGILFEEIMNESQQFAKEFPEAKW
                     RYTADKGLPEHLNEMLDRTMDRVNLQMRERREKSLAYLATISNIAPFLGVLGTVIGII
                     NAFTAIGKMGSAELAVVAPAISEALIATALGISVAIPASVGFNIFQYRVEILSQKFDR
                     FSALLRNRLENELVSEHESLADIGNESF"
     gene            3293..3712
                     /locus_tag="EYO68_10380"
     CDS             3293..3712
                     /locus_tag="EYO68_10380"
                     /inference="COORDINATES: protein motif:HMM:PF02472.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIC08246.1"
                     /translation="MIKVIGDQKKIVSEINITPLVDTLLVLLIIFMVTAPAMTRSVGI
                     ELPEPKHGSAAERVSQEKKNFAVIGINTKKQFIFEKKEYSPKDFFQKFPMLIEGLEPE
                     KIFLHADKQVVYEQIIELMTFLQSQGHEKIGLVFQEK"
     gene            3716..>4038
                     /locus_tag="EYO68_10385"
     CDS             3716..>4038
                     /locus_tag="EYO68_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08247.1"
                     /translation="MDSQSFVGEKHLTSSRNLGLAISFAMHVFLGSGFVALNTVTAEQ
                     ADFESLEVQSLEVNTLSADELQAIIALLELDPEVSEEGTNLPVIPVKEITASATLIGA
                     KSNSES"
BASE COUNT         1354 a          719 c          827 g         1138 t
ORIGIN      
        1 acaaactgcg gcctgcggcc tgctatacct cttaaaagtc gtccgagtaa atctgcaccg
       61 gtgataatgc gtttttccgt atcccaacac aaaatcagat cattgtcaat cacatcgtct
      121 tcagagtgtt ccggatgaac acgtaatttt tgaataactt cacccagtgg agttgcagca
      181 tcagatacca caatagggcg atgacagtaa gaatatggtc taaaaggatc aacaccaaaa
      241 agtgcatcgc gtaaaaataa atctgcattg agtaccaatc ggggttttcc atcaacagaa
      301 gttaaaataa cccatttttt ccctgatagc tgaacctgga gtaaaaacgg atcatccgga
      361 gtacgttgaa aagcaggaaa aaccggaaga tcaaattcag tttccagggg aataatactt
      421 accggatcga ctacctcacc ctcatctcca accggtaaat catcaatact taaaaaattc
      481 agtgctccgg ttccttccag tcggctgata tctgattccc ttgattccat gtgcttttgc
      541 agcatgattc ggatatcttc ctctttaaaa tacagcaccc cctcttttcc cagccacatg
      601 tcaagcaaca tggcagatgg tttactaacc ggatataaaa tgaatctgta aaatttaatt
      661 accggtgtca gcagtattcc cattcgcaag gcattacggg aaaaatatgc ttgaggcaag
      721 atctcaccaa ataaagtgat gcaaaatgag gaaaagaaaa aagcccaaag tccggcaagg
      781 actgagtctg ttaacagggt cagcaaaaca ttaatgctta cattgcccca caaaagggtt
      841 gtcagcagca gatttgcatc tttccttagt tcaagtacct cgactgcagc ctgattgcct
      901 gactcaactt ctatttcaag cttcagtcgg ctcaagccaa ataaccccaa agtcaagccg
      961 gaaaatactg cagattgagt taaacaaaag aaaatacccg ccaggagggt taggtccata
     1021 caactgtata ttagtaatta atcatgtaca caaactccga ttgaattata ttttgaaaac
     1081 atacactaac acgtctcttg aagttctcaa gttttttcag tacttcgcta ttcatcatag
     1141 tttaacagcg ggtcaattga agctgaataa aatcacgaaa taaatgttgc attaaaatag
     1201 atttgtatta tcatgtagcc taaattgatc atttttattt tcaggtcggt tcaaaaaagt
     1261 ttgattatta attagtgtta acgtatttta aaaaggctga gaacaatatt tgactggaat
     1321 tttaattgaa ttactgaaaa aatactgcat actttgaatt ttttctttaa accatcaccc
     1381 tttcgtggag attgaacatg cttaagtata ctctaaaagt actgattccc ctaactatta
     1441 tctttcttgt tagttgtgcc ccgaaaggag tctcacctgt tccaaagagt attatggcag
     1501 atttagatga ggtacgctca ctgactgaaa cggaattggc ggacaataaa aaaattcaaa
     1561 atgactacaa ccggaaacaa gaattattga aaaaaattgc tgttgaagat gtggcagaga
     1621 aaaaagaact gcttaaggaa aaaggggttg tggaaaaggc taaaaaggaa ctgtcagaat
     1681 tgaccgagaa gatccttaaa aaacgacgca aaaagctttt gaaggctgaa gttgaaaaaa
     1741 ttgctgaatc cctcaaggaa ggagtaacag taaaggaaat cgtggaactt ctggaaaaag
     1801 tagcttcaga tgaaatttca gtagaggaag caacagttat acttaagaaa aaaaccacat
     1861 taagtgatga aggaattaaa aagctgctcg caaaaactaa agctatccaa aaagaaactg
     1921 aggaacttgc cgaaaaactg gcaactgctt ctgcagatga ggtagcagaa attttaagag
     1981 aggcaggtgg cgtttcagaa aaagacattt tggctctagt cgaaaagaaa aaagaagtag
     2041 atgctataga gtcatctttt ctggaaaaaa caatggccaa actatacacc cgccttattc
     2101 gagatagaga acttggaatt gaagaagcat tctgcatcaa cattgaagga atattggatc
     2161 atctgttggt tgaaccttct tattttgata cagataaata ttcaattgtt gaatatatag
     2221 atcaaatcga aagttctttc agattgctgg aaccaattct agaggagtac cccgaaatta
     2281 tagtcaggtt ggaaggaaac gctgatgagc gcggtacggt tgaatataac aaagctctta
     2341 gtgatcgacg gtgggaaact ccttcaaaag ttttattagc tctctatttt gatgaagaca
     2401 ggacagctgg tgtcggtcgg agtgagcaat gtccattgcc acgtgcagga ggtatttcca
     2461 ctcgagattg gtggtcatca aaccggcgta ccgattacat ttttaagctg aaataatttt
     2521 aaactatgga atttaatgat tttgtaaatg cggtccttca cggtgggaca attacgcttt
     2581 ccatatttat tttgctcgtt gtcctttctg ttgtaacttg gggaattata atagggaaat
     2641 ttgttcagtt aaggaaggaa gatcaaaaca gtggtaaatt tgtaaaagta tttaataagg
     2701 caaaatcact caaaagtttt ttatcgacaa atcacaagcg tgctccggtg gattatgatt
     2761 tggggatttt gtttgaagaa attatgaatg agtcgcaaca atttgcaaaa gaatttcctg
     2821 aagcaaaatg gagatatact gcggataagg ggcttccaga acacttaaat gagatgctgg
     2881 accgaacgat ggatagggta aacctgcaaa tgcgggagcg ccgcgaaaaa agtttggcat
     2941 atttagcaac tatttccaat attgctccat tcctcggagt tttgggaact gtgataggaa
     3001 ttatcaatgc atttactgca attgggaaaa tggggtcagc agaactagca gtagtagctc
     3061 ctgcaatttc agaggcgttg attgctacag cattggggat ttctgttgcc attccagctt
     3121 cagtagggtt taacattttt caatatcggg tagaaatcct ttcacagaag tttgaccggt
     3181 tttctgcact tttgagaaac aggctggaaa atgaacttgt ttcagagcat gaatctttag
     3241 cagatattgg taatgaatca ttttaaatac tgaatttatt ggaggtaaca aaatgattaa
     3301 agtaattgga gaccaaaaaa aaatcgtctc agaaatcaat atcacccctc tggttgatac
     3361 cctcttggtc ttgttgatta ttttcatggt tactgctccg gcaatgaccc gctctgttgg
     3421 tattgaactt cctgaaccaa aacatgggtc tgcagcagaa cgggtctctc aagagaaaaa
     3481 gaattttgca gtcataggaa ttaatactaa aaagcagttt atttttgaaa aaaaagaata
     3541 ttctccaaaa gatttttttc agaaatttcc gatgcttatt gaaggtcttg aaccggaaaa
     3601 gatatttctc catgcagaca aacaggtagt atacgaacaa ataatagaac taatgacctt
     3661 tttacaaagc caaggccatg aaaaaatagg attggtgttc caggaaaaat aagaagtgga
     3721 tagtcaaagt tttgttggag aaaagcatct aacatcttcc agaaatttag ggctggctat
     3781 ttcttttgcc atgcatgtat ttctgggatc aggctttgtt gctcttaata ctgtaacagc
     3841 agaacaagct gattttgaat cacttgaagt tcaatctcta gaggttaata ctctgtcggc
     3901 agatgaactt caggcaataa ttgctttatt ggagttagat ccggaggttt cagaagaggg
     3961 tacaaacttg ccggtcattc ctgtgaaaga gataaccgca tcggcaacgc taattggggc
     4021 taaatccaat tctgaatc
//
LOCUS       DTVD01000283           14951 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_809_length_14951_cov_773.896952, whole genome
            shotgun sequence.
ACCESSION   DTVD01000283 DTVD01000000
VERSION     DTVD01000283.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 14951)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14951)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14951
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_14951_cov_773.896952"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="plsY"
                     /locus_tag="EYO68_10390"
     CDS             complement(<1..619)
                     /gene="plsY"
                     /locus_tag="EYO68_10390"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIC08248.1"
                     /translation="MFQIGFFILIFLLGSIPFGLLISRYWLKIDIRRQGSGNIGMTNV
                     MRVGGKWPGIVTFVLDFGKGILAVLTAQILFPVSETEPETQLIFHSLAGITVVCGHVF
                     SVFLRFKGGKGISTLFGVLAILQLNVGICAALAWAGMYLWKRISSLSAITMLAVLPWL
                     FLLVPWVQDERPVWSMFLIFLLLSFLLIYKHHENIQRLLKGQEGQL"
     gene            complement(646..1557)
                     /locus_tag="EYO68_10395"
     CDS             complement(646..1557)
                     /locus_tag="EYO68_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HIC08249.1"
                     /translation="MSTKLFKGTPTYLLDQKLSEAFHIARLLEKPLLLEGEPGTGKTK
                     LAQEFAAQGNHPIFEVPVTSESKVSLLVSRFDEVQRLMDTQAAVANAQMKQAGIDTQI
                     DTGGRKVDNLDEYVHLGPLAQAFSKPGSVLLLDEIDKAPRELPNNLLFLLSERRIVVP
                     ETQQTIECEPGKMPIIVITSNHEQDLPAPFIRRCIYMYIEFPPPERMREIIRMHHPGA
                     NKKIVAAAIEIFYQLRELDLTRKPSTSEILDWISYLVQTNVLDSKVVERLKGAQTLVK
                     HRDDRELLRAIQEKGIESATSRKSQNW"
     gene            complement(1601..2359)
                     /locus_tag="EYO68_10400"
     CDS             complement(1601..2359)
                     /locus_tag="EYO68_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08250.1"
                     /translation="MPVNYLNIYVFAIFGGLAATVGGIFICYTGMSETSYLVYRGMEL
                     TTYYLDKNRHDLYLNGLVYSITFGIAFLLLLAVIVIPSPEQIQKRLAATSFAGSPGST
                     EISVPEGETPDAEDTSPLDGESMEIELEPDIESYEVDEEEEEESALTGGIDDDDSDVV
                     YGTGRITDEAHKSFIISSPDSAVKFLMRKELDGRPLKSSEDEIYEAWQERGLSRGKLR
                     KHILKIMEWESVPELEVNEIYNQVKDKAYEISHS"
     gene            complement(2514..3305)
                     /locus_tag="EYO68_10405"
     CDS             complement(2514..3305)
                     /locus_tag="EYO68_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIC08251.1"
                     /translation="MDLKLKGKVAMVAASSKGLGYGIAQALAQEGASISIGSRTQEDI
                     ETAAEQLRNETGVPVLASVLDSSKPESIRQWTDATLNEFGGVDKLVVNAGGPPTGKFE
                     DFADEDWQAAFDLNLFSAVRMIRATLPLMRKRGGGSILTVTSMSVKEPIDVLILSNVM
                     RSGVTSLVKSLSIQLAPDNIRLNNLMPGRINTDRVRSLDALNSEKQGIPIEQLKTANQ
                     AQIPLGRYGTIEEFGRTGAFLLSDAASYITGSSLAVDGGVLKTVW"
     gene            complement(3348..5048)
                     /gene="lnt"
                     /locus_tag="EYO68_10410"
     CDS             complement(3348..5048)
                     /gene="lnt"
                     /locus_tag="EYO68_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIC08252.1"
                     /translation="MNISPEIKNQIPNTQSLFWLGSLISGILLGLSAPGFGTNWVGIL
                     AFFPLLAVLDQLHKNRLFSLQKRAGLFFAVCWFSGSIAASIGGYWITSSINVFGHIPW
                     FGALLITAVGYGLEVGLQLFVYFGIPLLFIRQRSGWDLIVRLAFVLALDPWYPRLIQW
                     NYGGLTFSKFPWLEQAADILGASGLLLYSAGFSFLLLYWWRWKSEGTGNRKSLFKAST
                     IYLILWVFGLGYGAWRTQDLVIQNSKLDSASSGNIQNLVPDFPTKLTVMVIQPNFSLQ
                     ELASNPELAHSKRQQNLAGLLEDSLKALEQLPENTTTPKLLIWPESVYPDPFFKVAAS
                     REKVLSFAEANQTSILLATVDWVKTQTGYKFHGVSVLIGANGEVLGRYNKIFLIPFGE
                     TIPFSEWFPGIASWLRNNIANMSEFERGREYTVFSVPENIEFSAPICFDIFSPTVVRG
                     MVRNGSNLVVNLSNLAWFGRTTASDNMVAVLRWRAIENRVPVVFASNNGKSVFIGADG
                     ENRSRQLGLFEEGSLTSTINIRPQFSFFREYAEWVWGGFMFLFFMLVILAQVRGKIFH
                     "
     gene            5159..5392
                     /locus_tag="EYO68_10415"
     CDS             5159..5392
                     /locus_tag="EYO68_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08253.1"
                     /translation="MISLTPYSLENPVEVSGEDYNKLVQMKEKGWSHCDSKEECLAKL
                     HYLRSGFSQGKISIGDFNEREKKLVIGYWNRGS"
     gene            complement(5438..8641)
                     /locus_tag="EYO68_10420"
     CDS             complement(5438..8641)
                     /locus_tag="EYO68_10420"
                     /inference="COORDINATES: protein motif:HMM:PF12796.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08254.1"
                     /translation="MRNRQMVFPLQPMFPPMSKDYPSWMFRKFQNFQFCSSLASFIRI
                     FLLFSLIYTQAAGLIFLPSGFSLPSPAFAQTSTNKTDSKVSHRLENFLISTEDRDDWT
                     RLMLAILNSVPAVEQVLAQGVSVNIRGTEEFHGVTPLIFASGIGSLVIVKLLVEKGAR
                     LNDTDTQGNTALIQAAKKGHFDTLQYLLLQGANPNAETNQKWKALMFAAHRGQFEVLK
                     TLLKHGARADFQDDLGMSSIMYAAQNGHFEIVLHLLKRGAKVDLASLNGSTALIWAIR
                     AGHENIAELLLNAGAEINYLESEFQRSPLLLAAMYGRESTVKRLLSAGANVEAIDKQG
                     ANALLLALSNNHLKTANFLIEYGITINTADQKKYNPLMAAVSQGSFELTKSLLKKGAV
                     VNAQDFAGKTALMIAAGKGDMKIVKLLLREQGRLDFSDRYGRTAWMYAAEQQRISMVR
                     FLSNNEIDNHENLFYAVSHGLFKVVETLLELHANTGVRDSQGWTPLMWAANNGHAKIV
                     ELLLKGGTNINSSNGKSPNVSGWTPLMLAAGSGHLETVEFLTQNGADLTAEDEDSWTA
                     LTWASHKGYTDVVSFLVDNGANIDKLGSDGSTPLIWAASQGHDTVVRVLLTKGANYTL
                     KDGYGKSALAHAVLQGHFSIAKMLIIHGADINQDLLYAARRGYKNMVVFLLEESAEID
                     YLSPHNETPLNLASQGKHWETAKILLEKGANSALQNAQGQTSLMSAVLTGEAKMVNLL
                     INSTKTGSLNSELENADFLVNTPLMTAAVHGYLDIVKILLDNGAKVESRNRWKESALI
                     LAASKGFREIVRLLIQYGADLHSEDITEKTALLRAAQTGHLLVVEMLLAQGANLHINA
                     IDLGGEGGTALIQAARNGHNNVLQTLLKAGAIVDETELIFHHSALMVAALNGHKKTVD
                     LLLTAGANPALTDTSQRSALLLAATNNHLSVVIQLLDSGQSGIQTDSNGNGIWHLLAM
                     SDVENRNIPTSSNLKLNEEGVPTEMQVLFSRKILLDSINNNGETALMIAAKHGKMKLV
                     KNLLELGASHKILTPEGKSAFTFATASGNNELAKLLR"
     gene            complement(8586..9734)
                     /gene="ftsW"
                     /locus_tag="EYO68_10425"
     CDS             complement(8586..9734)
                     /gene="ftsW"
                     /locus_tag="EYO68_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIC08255.1"
                     /translation="MHTRYDLPLIGATLFLLSWGTVMIYSTGGVYADLQYDDGQYFLY
                     RHLMHILAGLVVLGVALYVNYHKWQQFSIWLMLVMLILLILVLIPEVGHEVKGGRRWL
                     RIGSLSLQPAELLKIVLIIYVASYLERKQELLAFFFRGLTPNFIVTGIYLFLVLLQPD
                     FGTVILLATTVLLMLYVGGGSPIQIATSLLGVGVIAGFLIASHTYRVRRMLSFLNPWD
                     DPQNSGFQIIQSFIALGTGGWLGKGLGESLQKRLFLPDAHTDFIFAIIGEELGFLWIC
                     VLIILFSVFIWRGYWIAWNAQDTFGKHLAFGATTILSLQIVINLFVVIGLLPTKGLPL
                     PFISYGGTSLVVTMFLTGLLLNISGRIDPTDEKPANGFSSTTNVPSNE"
     gene            complement(9807..11558)
                     /gene="menD"
                     /locus_tag="EYO68_10430"
     CDS             complement(9807..11558)
                     /gene="menD"
                     /locus_tag="EYO68_10430"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIC08256.1"
                     /translation="MNWNECWGEWIIEELVRHGTLCFCISPGSRSTPLTAAVARNDLA
                     KSVVFYDERAAAYFALGHARATGKPAVLICTSGTACANYLPAVIESSIEQIPLLVLTA
                     DRPPELRDTGANQAINQTHIFGTHVRWFFDPGCPGPEFTPAALLSTINHALSKTQRPL
                     SGPVHLNLPFREPFFEKNEAEKSIDLLLPPKTNCYVHFAISRKILPKKEILELIESRP
                     ESGILSIGRVPSNSLDSLRKLAEELGWPVFADVTSGLRLGNNCPNRITYYDQLLHDDL
                     ISSNWDIEAVWHIGFPPTSKRWLTRWEQSPPSQMVWIADHSERHDQSHNFRWIIEADI
                     TEFCEHLVDELRLEKRSAVNRSKTQKWLQTSEKAGDLIEKHFSTNSENEKINEFALVR
                     RLTQIIPETHGFFVGNSLPVRAVDMVGSEKGADIPTALNRGASGIDGLIASACGYSSG
                     MQKPVTLLIGDLSALHDLNSFKLLTDSPEPVLLVLLNNHGGGIFSFLPVARQTDIFEP
                     FFAAPHDYNFRKVSSMFNLNYFNPGSISAFETDYSKAIQANKSAVIEIISEREKNPQY
                     LESLRQKLVKSAFESSS"
     gene            complement(11555..12979)
                     /locus_tag="EYO68_10435"
     CDS             complement(11555..12979)
                     /locus_tag="EYO68_10435"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIC08257.1"
                     /translation="MLPANQPSIDYNLKVKPEKFQSLEDARCFLLKQVKEWDNSQNKP
                     IPENSGVVRLEASLESAHPLEWLTLQDCSQKIYWENRSQKEQFAGIGTALEIADGKDG
                     YENILQVISKILQDSPETVRFYGGMRFDSKASISPEWETWGTARFTIPLVELSIIEEN
                     VTLACNFLPGDKKNSKLQPLQNLLESLRTEDVIIPDNPLIGLERHDLPEYRQWCRLVE
                     QGLENISSGKIDKVVLARAAVFSLARDYDPTELLLQLGRHAPQAFHFCFQLAPNKAFL
                     GITPELLYRRSGTQIGSEAIAGTRPRGQTLEDDQRLADELRNSEKEQREHLMVLDRME
                     RVLKQFCRQTKRLSYLERLPLRHVQHLLSRMQGILRPNVNDSDLLPSFHPTPAVSGTP
                     DPEAREIIRELEPFDRGWYAGPVGWINRNQAEFAVAIRSGLLCGRTLRVYTGAGIVAG
                     SVPEEEWREIEVKLQSWKSLLGRS"
     gene            complement(12951..13673)
                     /gene="ubiE"
                     /locus_tag="EYO68_10440"
     CDS             complement(12951..13673)
                     /gene="ubiE"
                     /locus_tag="EYO68_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIC08258.1"
                     /translation="MTYTLPGTDDKSRYVEQKFDEIAQKYDRFNDLITFGMHRYWKRF
                     VVRQTGLHPEEHCLDLCCGTGDIAREVLRQYPASKVTGLDFSQKMLNIAKSKSSNNSG
                     IQYLLGDAMEIPFPDTNFDAVTIGYGLRNVPNLNGCLQEILRVLKPGGVLVSLDVGKV
                     RLPVIKELNNFYFFRIVPLIGNLLMPGQDMFQYLPNSSLEYPNQELLKRLMFEAGFQQ
                     VEIHDFVFGASTVHVACKPTIN"
     gene            complement(13698..>14951)
                     /locus_tag="EYO68_10445"
     CDS             complement(13698..>14951)
                     /locus_tag="EYO68_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIC08259.1"
                     /translation="VGAFAHAVQTILREDGAETACYLTRPYSHFGPKIVGQTWSTEDH
                     PSPLPLIEKFKPDLIIPQAVAWAEQPWAADLVKQGLPIFSPVGDAMQIEISRQESSEL
                     CRKYGIPVPASYHVQNRIEARQLMQDDPRPYVLKNPICSPFSPVHAIICESPEDTLGW
                     IERVDYAEGLFLQEYLGKEEAGHFVFISGGEIRSLVTNQEYKRAFTGDMGPLAGAPLA
                     GIVEQDPDDKFGLARELIHPLKPWFEKTGYTGPLQVTAIRKEGTWHAIEYNIRLGVTT
                     TALLLRMLKNPVDVLLKVVRDQVPVLDWHPEKFFGCTLTLAGYGYPYVVPSVPKLPVE
                     VSAPLECDLWWNEVDEEDGQLYMANHQNYEMGHRIADVNACASEMDSAVEQIEKEILK
                     LRCLGSYYRLDLKELTEKYSSLLTS"
BASE COUNT         4225 a         3341 c         3148 g         4237 t
ORIGIN      
        1 tgagttgccc ttcctggcct tttagcaggc gctggatatt ttcgtgatgc ttgtatatca
       61 aaagaaatga taacagaaga aaaatcaaaa acatagacca aactggtctt tcatcctgta
      121 cccagggaac aagtagaaat aaccacggaa ggacagccag catagtgatt gcggaaagac
      181 tggagattcg tttccacaaa tacatgccgg cccatgccag ggctgcacag atgccgacat
      241 ttaactgcaa aattgctaaa actccaaaca aagttgaaat cccttttcca cctttaaaac
      301 ggagaaaaac ggaaaaaaca tgtccacaaa caactgtaat tccagccaga ctgtgaaaaa
      361 ttagttgagt ttcgggttct gtttctgaaa caggaaaaag tatttgtgca gtcaagactg
      421 cgaggattcc cttgccaaaa tccaaaacaa aggtcacaat gccgggccac tttccaccaa
      481 cacgcattac attagtcatg ccgatgttgc cgctgccctg cctgcggatg tcaattttta
      541 gccagtagcg gctgatcaat aagccgaagg ggatgcttcc caataaaaaa attaaaatga
      601 agaagccaat ttgaaacatg tatttgtgtt ctagaaggaa agaatttacc agttttgtga
      661 ttttcggctg gttgcagact caataccctt ttcctgaatg gcacgcaata gctcacggtc
      721 gtcccgatgt ttaaccaggg tttgtgcgcc tttcagtctt tcaacaactt ttgaatcaag
      781 gacattggtc tgaaccagat atgaaatcca atctaaaatt tcgctggttg agggttttct
      841 agtaagatca agttcacgta actggtagaa aatttcaatt gcagcagcaa caattttttt
      901 atttgcacca ggatgatgca tccggattat ttcccgcatc ctttccgggg gtggaaactc
      961 aatgtacatg taaatacagc gccgaataaa aggcgccggc aggtcctgtt catggttgga
     1021 tgtgatcacg atgatcggca tcttcccggg ctcacattca attgtttgct gtgtttccgg
     1081 aacaacaatc cgtctctcgg aaagtaaaaa caaaaggtta ttaggaagtt cacggggggc
     1141 cttatcaatt tcatccagca gcaaaactga tcctggttta gagaaagcct gtgcgagcgg
     1201 acccaaatga acatattcat ccaaattgtc aactttgcgt ccaccggtat caatctgcgt
     1261 atcaattccg gcctgtttca tttgcgcatt agccacggca gcttgggtat ccatcaatcg
     1321 ctgaacttca tcgaagcgtg aaacgagtag agaaactttg ctttctgaag ttactggaac
     1381 ctcaaaaatg gggtgattac cttgtgcagc aaactcctga gcgagttttg tttttcctgt
     1441 tcctggttca ccttccagca acagtggttt ttccagcaat cgtgcgatgt ggaaagcttc
     1501 acttaatttt tggtccaaaa ggtaagttgg ggttcctttg aaaagctttg tgctcatata
     1561 ttttaactag ctagtggctg caaatgactt gttcagtggg ttaagagtgt gaaatttcat
     1621 aagctttatc tttaacctgg ttatagattt cattaacttc caattccggt acgctttccc
     1681 attccattat cttgaggata tgcttacgca atttccctct ggaaagtccc cgttcctgcc
     1741 aagcttcata aatttcatcc tcgcttgatt tcagtggtcg tccatcaagt tctttacgca
     1801 tcagaaactt aacggcactg tccggactgg agatgatgaa ggatttgtgc gcctcgtctg
     1861 tgatacgtcc tgttccataa accacatccg aatcatcgtc atctattcca cccgtcagtg
     1921 cagattcttc ctcttcttct tcgtcgactt cataagattc tatgtccggt tccaactcta
     1981 tttccatgga ctctccatcc agtggcgaag tgtcttctgc atccggagtt tctccttcag
     2041 gaacactgat ttcagttgat cccggagaac ccgcaaacga agttgcagcc agacgttttt
     2101 gaatttgttc cggactggga atcacaatga ctgcaagaag aagcagaaac gcgatgccga
     2161 aagttatact gtaaaccaaa ccgtttagat acaggtcatg acggttttta tcgagataat
     2221 aggttgtgag ttccatgcct cgatagacaa gataactcgt ttcactcatg ccggtatagc
     2281 agataaaaat acctcctaca gttgctgcaa ggcccccgaa aatagcaaag acataaatat
     2341 ttagataatt gacaggcatt tgtacctcat tgattgatca agttaaatga atggaatttg
     2401 tcgtttagat tcaacctgaa cttcctttaa tttaaataga aaattcatgc ataaaacggc
     2461 agattcctct tgatcaagac tatcggcaga aatgcagaaa acttgactgt taattaccag
     2521 actgttttta gaactcctcc atcgacggcg aggctcgacc ctgtgatata actggcggca
     2581 tcggacagca aaaatgctcc ggtacgtcca aactcttcaa ttgtaccata acgtcccagt
     2641 ggaatttggg cttggtttgc cgtctttaat tgctcaattg gaattccttg tttttcagag
     2701 ttgagagcat ccaggctacg tacccggtct gtgttgatac gtccgggcat cagattgttg
     2761 aggcgaatgt tgtccggagc caattgaatg gaaaggcttt tcaccaaact ggttacgccg
     2821 gaacgcatga cgttagagag aatcaaaaca tcaattggtt ctttgaccga cattgaagtg
     2881 actgtcaaaa ttgagccgcc tccgcgtttc cgcatcaaag gcaaagttgc ccggatcatt
     2941 cttactgcac tgaaaaggtt gagatcaaaa gcggcctgcc agtcttcatc tgcaaaatct
     3001 tcaaattttc ctgtaggcgg tcctccggca tttacgacaa gtttatccac accaccaaac
     3061 tcattcagtg tggcatctgt ccattgtcga attgattcag gcttagatga atccagtacc
     3121 gaagccaaaa ctggtactcc ggtttcgttc cggagttgtt ctgcggcagt ttcaatatct
     3181 tcctgagttc ggcttccgat tgaaatagaa gcaccttcct gagccagtgc ctgtgcgatt
     3241 ccataaccca gacctttgct ggaagccgcc accatggcta ctttcccttt gagttttaag
     3301 tccatttttt ctccttgttt gtaaaagata gttaaaaggc aaaatgatta atgaaatatt
     3361 ttaccacgta cttgtgccag aattaccagc ataaaaaata aaaacatgaa tcctccccaa
     3421 acccactcag catactcgcg aaaaaatgag aattgtggtc gaatgttaat tgtggatgtt
     3481 aacgatcctt cttcaaataa tcccagttgt cggctccggt tttccccgtc tgcaccaatg
     3541 aacacacttt taccgttgtt ggaggcaaag actaccggaa cccgattttc gatggctctc
     3601 cagcgtaaaa ccgcgaccat gttatctgac gcggtggtgc gtccgaacca agctaagtta
     3661 ctgaggttta ccaccaaatt tgagccgttg cgtaccatgc ctctaactac tgtgggtgag
     3721 aaaatatcga agcaaattgg cgcagaaaac tcaatgtttt caggaacaga aaaaacggtg
     3781 tattcccggc cccgttcaaa ctctgacatg tttgcaatat tatttctgag ccacgaagct
     3841 attccgggaa accactctga aaatggaatt gtctctccaa atggaattag gaatatcttg
     3901 ttgtaacgtc caagaacttc accatttgct ccaatcagga ctgagactcc atgaaatttg
     3961 taaccggttt gagtctttac ccaatctaca gtagccagca aaatggaagt ttggtttgct
     4021 tcagcaaagg acaaaacttt ttcgcgtgaa gctgcaactt tgaaaaaagg atcaggatac
     4081 accgactcag gccaaattaa caactttgga gtggttgtat tttccggaag ctgctccagt
     4141 gccttgagtg aatcctccag caagccggcc aaattttgct gacgttttga gtgtgcaagt
     4201 tcagggttgg aagcaagttc ctggagggaa aaattaggct gtataaccat aactgtgagt
     4261 tttgtcggaa agtcaggaac aaggttttga atattgccgg atgatgcgga gtcaagtttt
     4321 gaattttgaa taacaaggtc ctgagttctc caggcaccgt aacctaagcc gaagacccac
     4381 aaaattaaat aaatggttga ggctttaaat aaggatttgc ggtttccagt tccttccgat
     4441 ttccagcgcc accagtagag cagcaaaaat gagaatccgg cgctgtagag aagaagtccg
     4501 gaagcaccaa gtatatctgc ggcttgctca agccatggaa atttagaaaa agtcaatccg
     4561 ccataattcc attgaatcag ccttggatac catggatcaa gagcgagaac aaatgcgagc
     4621 cgtacgatta aatcccaacc gctgcgttga cggatgaaaa gtaatgggat tccaaaatat
     4681 acaaaaagct gtaatccaac ttccagtcca tatccaacgg cagtaatcag cagtgcccca
     4741 aaccagggaa tgtggccgaa aacattgata ctgctagtaa tccaatatcc accgatcgaa
     4801 gcagcaatac ttccggaaaa ccagcagact gcaaaaaaaa gcccggcccg tttttggagt
     4861 gaaaacagcc tgtttttatg cagttgatcg agtaccgcca gcaatgggaa aaaagccagt
     4921 attccaaccc agttagtgcc aaagcccggg gcactcaggc cgagcaaaat gccggaaatg
     4981 aggcttccta gccaaaacag ggattgcgta tttggtattt ggtttttgat ttctggagaa
     5041 atattcatgg ttttttacag cagacttgca agctgttttc tttagtgctt aaaatggtat
     5101 taatattttt ttattgcaag ggtttccccc gtttttcgca gcaattattg tagatcttat
     5161 gattagttta acaccatact ctctggaaaa cccagttgaa gtttcagggg aagattataa
     5221 taagctggtc caaatgaagg aaaaaggctg gagccattgt gattcaaaag aggagtgcct
     5281 cgcaaaactg cattatctcc gttctggatt ttctcaaggc aaaatttcta taggagattt
     5341 caatgagcga gaaaaaaaac tggtgatcgg ctactggaat agagggagct aaaattcggt
     5401 tttgggtaca agtcctctgc tttaatatca taagtgatca tcgcaatagc ttggcaagct
     5461 cgttgtttcc ggatgctgtt gcaaatgtaa aggcactctt tccttcgggc gtcagtattt
     5521 tgtgagacgc accaagttca agcagatttt taacgagctt catctttccg tgttttgctg
     5581 caatcatcag tgctgtttcc ccattattgt ttatagagtc cagtaaaatt ttacgtgaga
     5641 aaagcacctg catttccgtt ggcacacctt cttcgtttag ttttaaattc gaactggttg
     5701 gtatgttcct attttcaaca tcactcattg ccagcaggtg ccagataccg ttgccgttgg
     5761 aatcagtttg aattccactt tgtccggagt ccaaaagctg tatcacaacc gaaaggtgat
     5821 tgtttgtagc tgcaagcaat aaagctgacc tttgtgaagt atctgttaac gctggatttg
     5881 ccccggcagt gagcaggaga tccactgttt tcttatgacc attcaaagca gcgaccatca
     5941 gagccgagtg gtgaaaaata agctctgtct catctacaat tgctccggct ttcaaaagtg
     6001 tttgcaaaac gttgttatgg ccgtttcttg cagcttgtat caaggcagtt ccaccttctc
     6061 ctcctaaatc aattgcgtta atgtgcagat ttgcgccttg tgcaagcagc atctcaacta
     6121 ctaacaggtg tccagtttgg gctgccctca ataaggcagt tttttcagta atatcttcag
     6181 agtgcaaatc tgctccatat tgaatcagca acctgactat ctcacgaaaa ccttttgaag
     6241 ctgcaaggat caatgccgat tctttccaac gatttctact ctcaactttt gcaccattat
     6301 cgagcaatat tttaacgata tctaaataac cgtggacagc agctgtcatc aaaggtgtgt
     6361 ttactaaaaa atctgcattt tcaagttcag aattcagaga gcctgtttta gtagagttga
     6421 tcagtagatt taccattttt gcttcaccgg tcaaaactgc agacatcagt gaagtctgtc
     6481 cctgggcatt ttgcaatgcg gaatttgcac ctttctccag aagaattttg gctgtttccc
     6541 agtgttttcc ctgactggca agatttaaag gagtttcgtt atgtggagac agataatcaa
     6601 tttccgcact ttcttcaagt aaaaatacaa ccatattttt gtatcctcta cgtgcagcat
     6661 aaagtagatc ctgattaatg tcggctccat gaataatcaa catttttgca attgagaaat
     6721 gaccttgcag tactgcatgt gcaagtgcgg attttccgta accatctttc aatgtataat
     6781 ttgcaccttt agttaacaag acacgcacta cggtatcgtg accctgagat gcggcccaaa
     6841 taagcggtgt agaaccatca gagccaagct tatcaatatt tgctccgtta tcaaccaaaa
     6901 agctaacaac atcagtgtat cctttatgag aagcccaggt tagtgctgtc caactatcct
     6961 catcttctgc agtaagatct gctccgttct gagtgagaaa ttctacagtt tcgagatgtc
     7021 cactccccgc agccagcatt agtggggtcc agccggaaac attgggactt tttccattac
     7081 tactgttaat atttgttcca ccttttagca gaagttctac gattttggca tgaccgttat
     7141 ttgctgccca catcaaagga gtccatcctt gagaatcacg gactcctgta tttgcgtgta
     7201 actccagcaa tgtttcaact actttgaata gtccatggga aacggcataa aagaggtttt
     7261 catggttatc aatttcatta ttgcttagaa atcgaaccat tgagatacgt tgttgttcgg
     7321 ctgcatacat ccatgcggtt cgtccgtagc ggtcgctaaa atcaagtcgc ccctgttctc
     7381 tgagcaggag ctttactatt ttcatatctc cttttcctgc tgcaatcatt aatgcggttt
     7441 ttccagcaaa atcctgagca ttcactaccg cccctttctt gagcagggat tttgttaatt
     7501 caaaacttcc ttgagaaact gcagccataa gagggttgta ttttttttgg tcagcagtgt
     7561 tgatagtaat tccgtactcg atcaaaaaat ttgccgtttt caaatgatta ttgcttagag
     7621 ccagtaacaa agcatttgca ccttgtttgt caattgcctc tacattagct ccggcggata
     7681 acaacctctt tacagtcgat tccctaccat acattgctgc cagcagcagt ggtgaccttt
     7741 gaaactcact ctccaaataa tttatttctg caccggcatt caagagtaat tcagcaatat
     7801 tttcatggcc cgccctaatg gcccaaatta aagctgttga accgttaagt gaggctaaat
     7861 ctacttttgc gccgcgcttt agtaagtgta ggactatttc aaaatgcccg ttctgagctg
     7921 catacataat agaggacatc cccaaatcgt cctggaagtc tgctcttgca ccatgtttca
     7981 ataaagtttt gagaacttca aattgtcctc tgtgtgcggc gaacattaat gccttccact
     8041 tttgattagt ttcagcattc gggtttgcac cttgcaggag taaatattgt agcgtatcaa
     8101 agtgtccttt ctttgccgcc tgaatcaaag ccgtattgcc ttgagtatcc gtatcattca
     8161 ggcgtgcacc tttttctaca agcaatttta ctatgactag ggaaccaatt ccggaagcaa
     8221 atataagcgg agtcactccg tgaaattctt cggttcctct aatatttacg cttacacctt
     8281 gtgccaaaac ctgctctact gcaggcacac tgttaagaat ggctaacatc aggcgagtcc
     8341 agtcatctcg atcttctgtg ctgattaaga aattttccaa gcggtgactt accttggaat
     8401 cagttttatt tgtggaagtt tgcgcaaagg ccggagaagg taatgaaaat ccggaaggga
     8461 gaaaaattag acctgctgcc tgtgtgtaaa ttaaggaaaa aagcaggaat atacggataa
     8521 aagatgccag tgaggaacag aattgaaaat tctgaaactt ccggaacatc caggatggat
     8581 agtccttact cattggaggg aacattggtt gtagaggaaa aaccatttgc cggtttctca
     8641 tcagttggat ctatacggcc gctgatattg agtaaaagac ctgttaagaa catggttacc
     8701 acaagagatg ttccaccgta gcttatgaat ggaagtggca aacctttagt tggaaggaga
     8761 cctataacaa caaaaagatt gataacaatt tgcaggctta gaattgtagt ggctccaaaa
     8821 gccaggtgtt ttccaaatgt atcttgggca ttccaggcta tccagtaacc gcgccaaata
     8881 aaaacggaaa atagaataat cagtacacaa atccacaaaa accctaattc ttctccaatg
     8941 atggcaaaaa taaaatctgt gtgagcatca ggcaggaaaa ggcgtttttg aaggctttct
     9001 cccaggccct taccaagcca cccacctgtt ccaagagcaa tgaatgactg gataatttgg
     9061 aatccggaat tttgcggatc atcccaggga ttcaaaaaag agagcattct ccgtacgcga
     9121 tatgtgtgtg atgcaatcaa aaatcctgca ataacaccca cacctaacag gctggtggca
     9181 atttgaatgg gactgccacc gccgacatac agcatcagca agactgttgt tgctaacaaa
     9241 attactgtcc caaagtctgg ttgcagcaag acaagaaaga gataaatacc tgtgacaata
     9301 aagtttggtg tcaacccgcg gaagaaaaaa gccagcaatt cctgctttct ttccaaatat
     9361 gaagccacat agataatcaa aactatcttc agcaattcgg caggctgcaa acttaacgaa
     9421 cctatacgca gccagcggcg tcctccctta acctcatgtc ccacttcagg aatcaaaacc
     9481 agaatcagta aaatcaacat taccagcatt aaccaaattg aaaattgctg ccatttgtga
     9541 tagttcacgt acagtgcaac tcccaaaact acaagtcctg ccagtatgtg catcaagtgc
     9601 cggtataaaa agtattgacc atcatcgtat tgaaggtcgg cataaacacc acctgtactg
     9661 taaatcatca ctgttcccca ggagagcaaa aataatgttg ctccaatcag aggaagatca
     9721 tagcgagtgt gcataaatat tatttagttt tcaacctgga atacttttaa agcaaaatag
     9781 ctaacaaatt tttaagtata acgttcttat gaagaagatt caaatgcact ttttacgagt
     9841 ttttgccgca aggactcaag atattgcgga tttttttcac gttccgaaat aatttctatt
     9901 actgcagatt tgtttgcttg aattgctttt gaataatctg tctcaaaagc actgatcgaa
     9961 ccagggttaa aataattcaa attaaacatt gaagaaactt tccggaaatt ataatcgtgt
    10021 ggtgcagcga aaaatggttc gaaaatatct gtctgccggg caacaggaag gaatgaaaaa
    10081 atacctcctc cgtggttatt gagtaaaacc agcaaaactg gttcaggaga atctgtgagt
    10141 agtttgaacg aattaagatc gtgtaaagct gacaagtcgc caataagcaa tgtgaccggc
    10201 ttctgcattc ctgaagagta tccgcatgct gacgctataa ggccgtcaat tccactggcg
    10261 ccccgattga gtgctgttgg aatatccgct cctttttcag aaccaaccat gtccactgca
    10321 cggactggga ggctgtttcc gacgaaaaaa ccatgagttt caggtattat ctgagtcaac
    10381 cttcgcacca aagcaaattc attaatcttc tcattttctg aatttgtgga aaaatgtttc
    10441 tctatcaaat caccagcctt ttctgatgtc tgaagccatt tctgcgtttt tgaacggtta
    10501 acggctgacc ttttttccag tcgcaattca tccactagat gctcacaaaa ttctgtaata
    10561 tcagcctcta ttatccagcg aaaattgtga gactgatcgt gacgttcaga atggtctgcg
    10621 atccagacca tttgagaggg agggctctgt tcccagcgcg taagccagcg tttagatgta
    10681 ggtggaaaac caatgtgcca aactgcttca atgtcccagt ttgaactgat aagatcatca
    10741 tgcagcagtt gatcatagta agttatcctg ttcgggcaat tattacccag acgtagtccg
    10801 gaagtgacat ctgcaaatac cggccaaccc aattcttctg caagttttct gagggaatca
    10861 agtgagtttg aaggaacacg tccgatgctc aagattccgg attcaggacg gctttcaatc
    10921 aattccaata tttctttttt tggcaggatt tttcttgaaa tcgcaaagtg aacgtagcag
    10981 tttgttttcg gcggcagtag taaatctata cttttttcag cctcattttt ttcaaagaaa
    11041 ggttcgcgga aaggtaaatt caggtgtact ggtccggaca agggacgctg tgtttttgat
    11101 agcgcatggt taattgtgga gagcaaggct gcgggagtga actccggtcc gggacatcct
    11161 ggatcaaaaa accagcggac gtgtgttcca aaaatgtgag tctggttaat agcctgattg
    11221 gctccggtat ctcgaagttc tggcggtcgg tcagcagtca gtaccagcag aggaatttgc
    11281 tcaattgaag attcgataac tgcaggcaaa taatttgcgc aggcagttcc ggatgtgcaa
    11341 atcaagactg ccggtttccc ggttgctctg gcatgaccga gtgcaaaata tgcggctgca
    11401 cgttcatcgt aaaatacaac agattttgcc aggtcattac gggcaaccgc cgcagtcaaa
    11461 ggtgtggaac gtgatccggg cgagatacaa aaacacaacg taccatgccg tactaattcc
    11521 tcaataatcc attctcccca acattcattc cagttcacga tcgtcccagt aaagatttcc
    11581 agctttggag tttgacttct atctccctcc actcttcttc cggaactgaa cctgcaacga
    11641 ttccagctcc tgtatagact cttaaagtgc gtccgcataa cagtccggat cgaattgcaa
    11701 cggcaaattc agcctgatta cggtttatcc agccaacagg acccgcatac caacctctgt
    11761 caaatggctc caactcccgg ataatctcac gagcttccgg atcaggtgtt cctgaaactg
    11821 ccggtgtcgg atggaaagac ggaagcaaat cggaatcatt tacattcgga cgcaggatcc
    11881 cctgcatcct gctaagcagg tgttgaacat gccgcagggg caggcgctcc aagtaagaca
    11941 agcgttttgt ttgccggcaa aattgcttca gtacacgttc cattcgatct aaaaccatta
    12001 aatgttcacg ctgttctttt tcactgttac gtagttcgtc tgccaaacgt tgatcatctt
    12061 caagtgtttg tccgcgggga cgtgttccgg caattgcttc actcccaatt tgtgttcccg
    12121 atcttcggta aagcagttct ggagtgatcc ccaaaaaggc tttattggga gcaagctgaa
    12181 aacaaaaatg aaaagcttgt ggtgcgtgac gtcccaattg gagcagtagt tcggtggggt
    12241 catagtcacg ggccaaactg aaaactgcag ctcgtgccaa aaccactttg tctattttgc
    12301 cgctggaaat gttttcaagt ccttgctcaa ccaggcgaca ccattgtcga tattctggaa
    12361 gatcgtgccg ctccagtcca atcagcggat tatctggaat gatgacatct tccgttcgca
    12421 gagactccag caaattttgc aacggctgaa gcttggaatt ctttttatca cctggaagga
    12481 aattacaggc caaagttaca ttttcctcga taatgcttaa ttcaaccagt ggtatagtaa
    12541 aacgtgcggt tccccaagtt tcccactctg gggaaattga agctttggag tcaaaacgca
    12601 ttcctccgta aaaacggact gtttcaggag aatcctgtaa aattttactg ataacttgca
    12661 atatattttc gtaaccatcc ttcccgtctg caatctcaag ggctgtacca ataccggcaa
    12721 attgttcttt ttgcgaacgg ttttcccaat aaatcttctg tgaacaatcc tgtaaggtta
    12781 gccattccag aggatgagcc gactctagag aagcttccag acgtacaact ccagagtttt
    12841 cagggattgg tttattttgg gaattatccc attctttgac ttgttttagt aggaaacagc
    12901 gggcatcctc aagcgattga aatttttctg gtttcacttt tagattataa tcaattgatg
    12961 gttggtttgc aggcaacatg tacagtggaa gccccaaaaa cgaaatcgtg tatttcaacc
    13021 tgctggaatc ctgcttcaaa cattaggcgt ttaagtaatt cctggttagg atattcaagt
    13081 gaagaatttg gcaaatattg aaacatatcc tgtccgggca tcaacaaatt tccgatcaaa
    13141 ggaacgatac ggaaaaaata aaagttattt agttccttaa taaccggtaa acgaactttt
    13201 cctacatcca ggctgaccaa aactccgcct ggtttcagca cacgcaagat ttcttgcagg
    13261 catccgttca ggtttggaac gtttcgtaaa ccgtacccaa tcgtgactgc atcaaaattt
    13321 gtatccggaa aagggatttc cattgcgtca cccagcagat attgaattcc tgaattattg
    13381 ctgcttttgg atttggcaat gttgagcatt ttttgcgaaa aatcaagacc tgtcactttg
    13441 gatgcaggat attgacgtaa tacttctcga gcgatatcgc cggttccaca gcacaaatcc
    13501 aggcagtgtt cttccgggtg cagtccagtt tgtcgtacca cgaacctttt ccagtagcgg
    13561 tgcattccaa aagtgataag atcgttgaat cgatcgtatt tttgagcaat ttcatcaaac
    13621 ttttgttcaa cgtagcgaga tttatcatca gttcctggaa gtgtgtaggt catcgatttt
    13681 tattttaaag cagcttttta ggaggtcaag agtgaggaat atttttcagt taattctttg
    13741 aggtccagtc ggtaataact tcccaaacag cggagtttga gaatttcttt ttctatttgt
    13801 tcaactgctg aatccatctc agaagcacag gcattcacgt ccgcaatcct gtgacccatt
    13861 tcgtaatttt gatgatttgc catatacagt tgaccgtcct cttcatctac ttcattccac
    13921 cacaagtcac actcaagagg tgctgaaact tcaaccggta attttggtac agaaggaaca
    13981 acgtaaggat agccgtatcc tgctaatgta agagtgcagc caaaaaattt ctccggatgc
    14041 caatccagta cagggacttg atcccggacg actttcagca aaacatcaac cgggtttttt
    14101 agcatcctca acagaagcgc ggttgtagtc acacctaaac gaatgttgta ttcaattgca
    14161 tgccaggttc cttctttgcg tattgcagtg acctggagcg gtccggtgta gcctgttttt
    14221 tcaaaccagg gctttagagg atgaattaat tctcgagcta aaccaaattt atcatccgga
    14281 tcctgttcta caattccggc caaaggtgca cctgccaatg gccccatatc tccggtaaaa
    14341 gcacgtttgt attcctggtt tgtaaccaaa ctgcgtatct ctccgccact gataaaaaca
    14401 aaatgtccgg cctcctcttt tccaagatac tcctgtaaaa atagaccttc tgcataatcc
    14461 acgcgctcaa tccagcccag cgtatcttcc gggctttcac agataatggc atggactgga
    14521 ctgaagggag agcaaatggg gtttttcaaa acataaggcc gcggatcgtc ctgcatcaac
    14581 tgtcgtgcct caatgcggtt ctggacatgg taggaggccg gcactggaat gccgtatttc
    14641 cggcacaact ccgaagactc ctgtcgtgaa atttctatct gcattgcatc accgacagga
    14701 ctaaaaatcg gcaaaccctg cttgaccagg tctgcagccc aaggctgttc tgcccaggct
    14761 actgcctgtg gaattatcag atcaggttta aatttttcaa tcaggggtaa aggagacgga
    14821 tgatcttcag tactccaggt ttgaccgact attttggggc cgaagtgact gtagggtctt
    14881 gtcaggtaac aagcggtttc tgcgccatct tcccgaagga tagtttgtac tgcatgcgca
    14941 aaagcaccca c
//
LOCUS       DTVD01000284            4019 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8149_length_4019_cov_141.006307, whole genome
            shotgun sequence.
ACCESSION   DTVD01000284 DTVD01000000
VERSION     DTVD01000284.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8149_length_4019_cov_141.006307"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="EYO68_10450"
     CDS             <1..443
                     /locus_tag="EYO68_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIC08260.1"
                     /translation="PMIAVTHCESAKLIKKFGAGVDGFLCPTQWAETLTYSGKHFGSA
                     AEYDKLFKATFDGYKNVPYQAAQASAAVLVWAEAFERANSFDTEALRDALAATDMKTF
                     YGDIRFSEAGNNIAKPMVLRQIQNGILNVVAPPEWASHAVDWPR"
     gene            527..1435
                     /locus_tag="EYO68_10455"
     CDS             527..1435
                     /locus_tag="EYO68_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08261.1"
                     /translation="MFDNLSLLGLVPLLNLQLLIDGILIGSIFALAAYGLALVWGVMN
                     IKNLAQGDFVIMGGYIAFTLSQSPFNLHPILTIPFVFVLMFVFGWVLYITIIRRVIER
                     DLFTSLLATFGLAIVIQQLINLAYGPDVQTVESGMAIREFFDGEITIADTKLVSFLLA
                     MSMALLITVFMKKSRMGRAIRATSQNARAARIMGIDTDRVYAFTFSLNAGICGIAGVL
                     ISIIWVIQPFFGMTHSIRSFVIVTSAGFGNLIGVILSALGLGAVEQFSGFILGAEFQQ
                     ATVVGLLVGVLIIRQIIQGRQRQVVK"
     gene            1432..2505
                     /locus_tag="EYO68_10460"
     CDS             1432..2505
                     /locus_tag="EYO68_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08262.1"
                     /translation="MNLKLKKILFYGGILLVGIFAPVVFPAYTFQLAILWLMILFALT
                     WDILGGQMGYNSLGNILFFGAGMYVCVVVQIGMYYDVAEYTAHFGAVKIDYTPYQYFS
                     GLALGLVLAALFSLVLAVIFGWIVFGLRGPYFAIGTLGVAIAAGELVGTWDYVGGGGG
                     ITVPMYPGDPDDRAVFFYSLSFVSAVSTFIFLKWLYSTRFQLAINAIRDDEEKAEAMG
                     IHTNRYKTIAWSFAAFFLGISGGIFGNMTGFIEPLEVAFPTVTFGIFMVLMVLLGGKG
                     TLWGPVIGAVLFHLIKEITWTYLLGWQWVALGSLIIINVVFFQQGIMGWVMEKWPDLF
                     GIKVDEDVVSAKGTNSVNNKVVS"
     gene            2502..3245
                     /locus_tag="EYO68_10465"
     CDS             2502..3245
                     /locus_tag="EYO68_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08263.1"
                     /translation="MSNIIEVRGVNKSFGGVVANHDISLDVQTGKITGLIGPNGSGKT
                     TLFNSIVGYHPIDSGTIQFADREISKMRVQKIARLGILRTFQQTRIYSEMDCVKNMLI
                     SAAHHHTGWFDMFRKYPQELTERAEHLLEFVGLFAKRHLISGDLSFGQQKLLEFAMAL
                     MNEPKVLLLDEPTAGINPTLINGLINRLKRANHEFGITLFVIEHNMRVVMNLADSIYC
                     LAHGEMLAHGTPEEIKNDQNVINAYLGAH"
     gene            3245..>4019
                     /locus_tag="EYO68_10470"
     CDS             3245..>4019
                     /locus_tag="EYO68_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC08264.1"
                     /translation="MPEDISPKNTKSESSFGYHDGEVENIISVEDVNRQAQEIAETVG
                     KEKIAGLVEGDPFLKIEGLHAGYGKMEILHDFNLQVGKGQSLCMIGPNGAGKSTVLHS
                     IFGFTNIYSGNILTRIGDTETTITQMSSSQKLKEAGISYILQDNSVFPDMTVEENLMM
                     GGYLKENTDAAKQSTERIFEKYDRLKARRNKPAKVLSGGERRLLEISRALVMEPNILL
                     VDEPSIGLEPRFIDMVFEILDDLQRNEGKTIIMVEQNAKK"
BASE COUNT         1012 a          811 c          985 g         1211 t
ORIGIN      
        1 tgccaatgat tgcagtcaca cactgtgaat ccgcaaagtt gattaagaaa tttggcgcgg
       61 gagttgatgg cttcctctgt ccaacccaat gggcggaaac ccttacatac agcggcaaac
      121 atttcggttc cgcagcagag tatgataagc tcttcaaagc aacatttgat ggttacaaaa
      181 acgttcctta tcaggcagca caggcatcag cagctgtcct tgtttgggct gaagcgtttg
      241 agcgtgcgaa ttcctttgat actgaagcac tgagggatgc actggctgcc acggatatga
      301 aaactttcta tggagatatc cgtttttcag aagcgggtaa caacattgcc aagccgatgg
      361 tgctgaggca gattcagaat ggcatactaa atgtggttgc tccacccgag tgggcgtctc
      421 acgctgttga ttggccccgt tgattttcat ttcctagtag tattttcaag cgttcccgtt
      481 taaacacggg agcgctttgc ttttcctacc acgaatctac aatttcatgt ttgacaactt
      541 aagcctgttg ggtttggtgc cactgctgaa tctgcagcta cttatcgatg gtattttgat
      601 cggttctatt ttcgctcttg ccgcctatgg tctggctttg gtctggggag tgatgaatat
      661 caagaatctt gcccagggag attttgtcat catgggaggc tacattgctt tcactctctc
      721 acaatctcct tttaatttgc atcccatcct gactattcct tttgttttcg tgttgatgtt
      781 cgttttcggc tgggtgttgt acattaccat catccgccgt gtgatagagc gggatctttt
      841 cacctcactt ctggctactt tcggcctggc cattgtgatt cagcaattga tcaatcttgc
      901 ctatggacct gatgtgcaga cggttgaatc aggcatggca atccgggaat tctttgatgg
      961 agaaattacg attgccgata ccaaattagt gtccttcctg ttggcaatgt caatggcact
     1021 cctgattaca gttttcatga aaaaaagtcg gatgggtcgt gctataagag ctacctcaca
     1081 gaatgcgcgt gccgcacgaa tcatgggcat tgacaccgac cgggtttatg ctttcacttt
     1141 ctcactcaat gcggggattt gcggaattgc gggagtttta atttccataa tctgggtcat
     1201 tcagccattt tttggcatga ctcattcaat cagatccttt gtaatcgtca catcagcagg
     1261 atttggcaat ctcattgggg tgattctttc tgctttgggt ctgggagcag tggaacaatt
     1321 cagtggattt attttaggtg cagaatttca acaggccaca gtggtaggac ttctggtagg
     1381 agttctcatt atccgtcaaa tcattcaagg ccgtcagcgt caggtagtta agtgaatctc
     1441 aaactaaaga aaatcctttt ttacggagga atactactgg tcggaatctt tgctccagtt
     1501 gttttccctg cttatacctt tcaactggca atactttggt tgatgattct gtttgcgttg
     1561 acctgggaca tccttggtgg tcagatgggg tacaattcat tgggtaacat cctgttcttt
     1621 ggtgcaggca tgtacgtctg cgtcgttgtc cagataggga tgtattatga tgtcgcagag
     1681 tataccgcgc attttggtgc agtaaaaatt gattatacac cataccagta cttcagcggc
     1741 ttggctcttg gattggtact ggcggcatta ttttctttgg ttttggcggt gatttttgga
     1801 tggattgtct ttggccttcg tggaccgtat ttcgcgatcg gtacgcttgg tgttgctatt
     1861 gctgccggtg agctggttgg tacatgggac tacgtagggg gaggtggagg tatcaccgta
     1921 ccaatgtatc cgggggatcc ggacgaccgc gcagtgtttt tttattctct ctcttttgtt
     1981 tctgcagtca gcactttcat ctttctcaaa tggctttatt caaccaggtt ccaactggca
     2041 atcaacgcca tccgtgatga tgaagaaaag gccgaagcaa tgggaatcca taccaaccgc
     2101 tacaagacta tcgcctggtc atttgcagca tttttcctgg gtatttctgg aggaatattt
     2161 ggcaacatga ctggattcat tgaacctctt gaagtggcat tccctactgt cacttttggc
     2221 atcttcatgg ttcttatggt attgctggga ggcaagggca ctctttgggg gcctgtcatt
     2281 ggtgcggttc tttttcactt gattaaagaa attacctgga cctatctgct tggctggcaa
     2341 tgggttgcac tggggagcct gattatcatt aatgtggttt ttttccagca gggaataatg
     2401 ggttgggtga tggaaaagtg gccggattta tttggtataa aagttgatga agatgtggtt
     2461 tcagctaaag gaacaaactc tgttaataat aaagtggtct catgagtaat attattgagg
     2521 tcaggggagt gaacaaatct tttgggggag tagttgctaa ccacgacatt tctcttgatg
     2581 tgcagactgg aaaaattacc ggtttgattg gtccgaatgg atccggaaaa actacccttt
     2641 tcaattctat cgttggatat catcccattg attccggaac catccagttc gcggatcgag
     2701 aaatttcaaa gatgcgggtg caaaagattg caagattggg gattctccgg acatttcagc
     2761 aaacaagaat ctactcagaa atggattgtg ttaagaacat gctgatatct gccgcgcacc
     2821 accatacagg ttggtttgat atgtttcgaa agtatcctca ggaattgact gaacgggctg
     2881 aacacttgct ggaattcgtt ggtctttttg caaaacgtca ccttatttca ggtgaccttt
     2941 catttggtca gcaaaagctt ctggagtttg ccatggcttt gatgaatgaa cccaaggtgt
     3001 tgcttcttga tgagccgact gctggcatca atcctacctt gattaacggg ctgattaatc
     3061 gtttgaaacg ggccaaccat gaattcggta tcactctctt tgttattgaa cacaatatga
     3121 gagtggtgat gaatctggca gacagtatat actgtctggc tcacggggaa atgctggctc
     3181 atgggactcc ggaagaaatt aaaaacgatc aaaatgtcat caacgcctac cttggagctc
     3241 attgatgcca gaggatatct ctcctaaaaa tactaaatct gaatcttcat ttggctatca
     3301 tgatggggaa gttgaaaaca tcatttccgt tgaggacgtc aatcgacagg ctcaggaaat
     3361 tgcagaaaca gttggcaaag aaaaaatagc cggacttgtc gagggtgatc cttttttgaa
     3421 aattgaaggt cttcacgcgg gttacgggaa gatggaaatc cttcatgact ttaacctgca
     3481 ggttggtaaa gggcagtcgc tctgtatgat tggtcctaac ggagcaggta aatctacggt
     3541 attgcattct atctttggtt ttacaaatat ctattccgga aatattctga ctcggattgg
     3601 tgatactgaa acgacaatca ctcaaatgag ttccagccag aaactaaaag aggccggaat
     3661 ctcctatatc cttcaggata attcggtttt tccggacatg acagtggaag aaaatctgat
     3721 gatgggaggt tatctaaagg aaaacacgga tgccgcaaag caatcaacag agcgcatatt
     3781 tgagaaatat gatcgactaa aagcaaggcg aaacaaacct gcaaaggtgc tctcaggagg
     3841 agaaagacgt ctgctggaaa tttcaagagc gttggtaatg gaacccaaca ttctgcttgt
     3901 cgatgaaccg tcgattggcc tggagccgcg tttcattgac atggtatttg agattctcga
     3961 tgaccttcaa cgaaatgaag gcaagaccat tatcatggtg gaacagaatg ccaagaaag
//
LOCUS       DTVD01000285            3987 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8243_length_3987_cov_234.999491, whole genome
            shotgun sequence.
ACCESSION   DTVD01000285 DTVD01000000
VERSION     DTVD01000285.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8243_length_3987_cov_234.999491"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            107..1072
                     /locus_tag="EYO68_10475"
     CDS             107..1072
                     /locus_tag="EYO68_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HIC08265.1"
                     /translation="MSSKPKVLATRTLPDAVEKRLAQDYNVRLNPDDALYSPDELIEL
                     AQEIDAILPCHTEHFTPDVVQRLPDSVKIVANFSVGVDHCDLEAFRKRGIIVTNTPDV
                     LSDATAEIAILLMLGAARRAGEGERMMRDGSWRDWSPSFMVGSAITGKRLGIIGMGRV
                     GQIVAKRARGFEMEIHYCNRNRLAETNEQGAIYHDSIEDLLPVSDVLSVNCPASPETA
                     NLLSAERIARLPDGAIIVNTARGALVDESALVDALRSGKIASAGLDVYRTEPGGNREI
                     AALPNTFLLPHIGSANMETRDAMGFRALDNLDAYFAGREPKDRVA"
     gene            1448..2581
                     /locus_tag="EYO68_10480"
     CDS             1448..2581
                     /locus_tag="EYO68_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIC08266.1"
                     /translation="MSNKTATDAKSVKKDVISRRKFMRGTAVAGVAAGTFAAPHIATA
                     ATVLKVQAAWGGGIFLENAKSYVDRVNKMAGGGLKIDLLSVNSVVKTSQMQDAVHRGV
                     LDGAHYVPAYWYSKSPAASLFGTGPCFGWSAQEMLGWIHYGGGMDLFNELMGSLKLNL
                     VSFFNSPMPAQPLGWFKEKISRSSQMKGLKYRTVGLAADVMNEMGLSVVQLPGGEIQP
                     AMKSGLIDAAEFNNPTSDSDFGMQDVSKHYHLASFHQSQECFEVTFNARKFNSLSKEQ
                     QAILEYASEAENSNFYWHNTLRYADDLIKLKDKHGVNVYRTPDSVMKDQLKAWDVVVN
                     RISSKDPFFAKVVKSQKSYAKKVMNYLLLNQPDYGIAYKHHFG"
     gene            2696..3271
                     /locus_tag="EYO68_10485"
     CDS             2696..3271
                     /locus_tag="EYO68_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIC08267.1"
                     /translation="MISIIHAIERLNIWIGRSFGWCILILTLSVAYEVFVRYVINAPT
                     VWAFDMMVQMYGALFLMAGPYALAQDTHVRADVVYRFLQPRWQALVDIILYILFFFPG
                     MLALFWFGWEIASDSWRYQEVSWNSPARIQIYFFKTLIPLAGGLFIVQGVAESMRCYL
                     AMKNNRWLPRQKDSRETEELLKGQADGVKID"
     gene            3317..>3987
                     /locus_tag="EYO68_10490"
     CDS             3317..>3987
                     /locus_tag="EYO68_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIC08268.1"
                     /translation="MTNPEVAILMLSSFIVMVLLGFPVAFTLLAMGVFFGYYAYLDAG
                     SIQTISDAFNNNIFYLLNQNTYSVMENDTLVAIPLFLFMGYVVERANIVNKLFYSLQM
                     AARNLPGSMAIAALITCTVFSTASGIVGAVVTLMGLLAFPAMIKANYDKSFSAGVICS
                     GGTLGILIPPSIMLIVYGAIAELSPLRLYAAAMIPGLLLAGSYIIYVIFRVMINPSIA
                     PKPRE"
BASE COUNT          984 a          948 c          975 g         1080 t
ORIGIN      
        1 cctgaatttc acattattga gccttgaatt ttcggcaagt gaagtgttat catgattttt
       61 tgcgtaccgg ttcatcgcat tcctcgataa tcaaaaagga tttttcatgt cttccaaacc
      121 caaagtactt gccacccgta cacttcccga cgcagtcgaa aagagacttg cacaggatta
      181 caacgtccgc ctaaatcctg atgatgccct gtattctccg gatgaattga ttgaattagc
      241 ccaggaaatt gatgccatcc taccctgtca cacagaacat tttactcccg atgtagttca
      301 acgactcccg gacagtgtga aaatagtagc aaatttttcg gttggtgttg atcattgtga
      361 cctggaggcg tttcgcaagc gtggtatcat tgtgaccaat acaccggatg tgctcagcga
      421 tgcaacggcg gaaattgcta ttttactgat gctgggcgcg gcgcggcgtg ccggcgaagg
      481 cgagcgtatg atgcgggatg gttcatggcg tgactggagc ccgtcattca tggtcggcag
      541 cgccattacg ggaaaacgct taggtataat cggcatgggc cgcgtggggc aaatcgtagc
      601 aaaacgggca cggggatttg agatggaaat acactattgc aaccgtaacc ggttagccga
      661 aacgaatgag caaggcgcca tttatcacga ctctattgag gacctgctac ccgtcagcga
      721 tgttttgtct gtcaactgcc ctgcatcccc ggagaccgcc aatctgttat ccgcggaacg
      781 gatcgcccga ttgcccgatg gcgcaattat cgtcaacact gcgcgcggag cactggtaga
      841 tgaaagcgca ctggtagacg cattgaggtc aggcaagata gcctcagcag ggcttgatgt
      901 ttaccgcacc gaacccggcg gcaaccggga aattgctgca ttacccaata cgttcttgtt
      961 gccccatatc ggcagcgcta acatggaaac tcgggatgcc atgggcttta gagctcttga
     1021 taatttggat gcttattttg caggccgcga gccaaaagac agagtcgcct gacagaaaac
     1081 caacaaaaca ttttttttgt taaatcagat atttgcaaaa taaaaacaac aaatcgacac
     1141 tagctccaaa attaatgtta ttttttgctt gacacaattc ccatatggaa ttaccatcct
     1201 ttctagactt ttttatttac cgtcaaagtt tgagttattt caaaattatc tttttatggt
     1261 ttcaataatt caaagctgaa gggattaaag aggaagtcta acgtaaaggg agacctgcat
     1321 agatttaact agatctccca tcgagatgct atcttggttc tctgaggggg gtcttttcag
     1381 tatctgcaaa ttctaatccg gtcatgtgca ggactcaccg ggtttaactg ataaatggag
     1441 aataggtatg tcgaataaaa cagcaactga tgcaaaatca gttaaaaaag acgtcatttc
     1501 acgccgcaaa ttcatgcggg gcactgccgt cgcgggtgta gcagctggca ctttcgctgc
     1561 acctcatata gccacagccg ctacggtcct gaaagttcag gctgcctggg gtggtgggat
     1621 ttttctcgag aatgcaaaaa gctatgtcga tcgagtcaac aaaatggccg gggggggtct
     1681 gaagatcgat ctgttatctg ttaactcggt tgtcaaaacc agccagatgc aggatgccgt
     1741 tcaccgtggt gttcttgatg gtgctcacta tgtacctgcg tactggtatt ccaagtcccc
     1801 ggcagcgtca ctgttcggta ccggcccatg ctttggctgg tctgcgcagg aaatgctcgg
     1861 ctggattcat tatggcggcg ggatggacct gttcaacgaa ctgatgggca gcctgaaact
     1921 taacctggtc agcttcttca acagcccgat gccggcacag ccgctgggtt ggttcaagga
     1981 aaagatcagt agatcttccc agatgaaggg cctgaagtac cgtaccgttg gcttggcggc
     2041 tgacgttatg aatgaaatgg gcttgtctgt tgttcagttg cctggcggcg aaattcagcc
     2101 ggcgatgaag agcggcctga tcgatgcagc cgagttcaac aatccgactt ccgacagtga
     2161 cttcggcatg caggatgttt ccaagcacta tcacttggct agcttccacc agtcccagga
     2221 atgcttcgaa gtcaccttca acgctaggaa attcaattct ctgtcgaagg agcaacaggc
     2281 gattctggaa tacgcttctg aagccgagaa ctcgaacttc tactggcaca acacccttcg
     2341 ttacgccgat gacctgatta agctcaagga caagcatggc gtcaatgtct accgcacgcc
     2401 ggattcggtc atgaaagatc aattgaaggc gtgggatgtg gtggtgaacc ggatttcgtc
     2461 aaaagatccg ttcttcgcca aggtagtcaa gtcgcagaag tcctacgcca agaaggtgat
     2521 gaactacctg ctcctgaacc agccggatta cggtatcgct tacaagcacc acttcggttg
     2581 atctaatctg tttttcttgc gagaccattt cgcaggatcg atcagttatg gagggacagc
     2641 cttgcggctg tccctccaat atctgaatta tcccaaaagg caggagaaaa aacccgtgat
     2701 atccattatt catgcaatcg aacgcctcaa catttggatt gggcgctcct ttggctggtg
     2761 tattctgatt ttaacccttt ccgtcgccta cgaagtcttt gtccgttatg tcatcaatgc
     2821 ccctacagtt tgggcatttg acatgatggt acagatgtac ggagcattgt tcctcatggc
     2881 agggccttac gccttagccc aggacacgca tgtacgtgct gatgtggttt accgcttcct
     2941 acaaccacgc tggcaggccc tggttgatat tattttatat atcctcttct tttttccagg
     3001 catgctcgca ctattctggt tcggttggga aattgcctca gactcatggc gttatcaaga
     3061 agtcagttgg aacagtcctg cccgtattca gatttatttc tttaaaacct tgatcccctt
     3121 ggcaggtggg ttgttcatcg ttcagggtgt agctgaaagc atgcgctgct atctggcgat
     3181 gaaaaacaac agatggctgc cacgtcagaa agattcccgg gaaacggaag aattactgaa
     3241 gggccaggca gatggagtaa aaattgatta aacctgttta cacaacctta actgttataa
     3301 tggagcgccc tttctcatga caaatcccga agtcgcaatt ttgatgcttt cctcgttcat
     3361 cgtgatggta ctgttaggat ttccagttgc ctttacgtta ttggcgatgg gcgttttctt
     3421 tggatattac gcttaccttg atgccggaag tattcagaca atcagtgatg ccttcaacaa
     3481 taatattttc tatctgctga accagaatac ctattcagtg atggaaaatg atacgctggt
     3541 tgcgatcccg ctgttcctgt ttatggggta tgttgttgag cgcgccaaca tcgtcaataa
     3601 gcttttctac tctctgcaga tggctgcgcg aaatctgccc ggatccatgg caatagcggc
     3661 cttaatcacc tgcacagtgt tttcaacagc aagcggtatc gttggtgccg ttgttaccct
     3721 gatgggtctg ctggctttcc ctgcaatgat aaaagctaat tatgacaaaa gcttctccgc
     3781 tggcgttatc tgctctggcg gtacgcttgg cattctgatt ccgccatcca tcatgctgat
     3841 agtctatggc gccatcgctg aattatctcc gttgcgcttg tatgcggcag ccatgatccc
     3901 aggtcttctc ttggcgggat catacataat ctatgtcatt ttccgggtta tgataaatcc
     3961 gtcaattgcg ccgaaaccac gtgaaga
//
LOCUS       DTVD01000286            3969 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8302_length_3969_cov_287.878385, whole genome
            shotgun sequence.
ACCESSION   DTVD01000286 DTVD01000000
VERSION     DTVD01000286.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8302_length_3969_cov_287.878385"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(381..1421)
                     /locus_tag="EYO68_10495"
     CDS             complement(381..1421)
                     /locus_tag="EYO68_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020026623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08269.1"
                     /translation="MRSTKIIHIVSCHAEGEVGDVIVGGVNPPPGDSIWEQSRWIEQD
                     QTLRRFVLNEPRGGVFKHVNLLVPPKSEKADFGFIIMEPEHTPPMSGSNSICVATVLL
                     DSGMIQMEEPETSFYLEAPAGLLQVKAQCENGKAVSIEIKNVASFADKLDVKLEVSGL
                     GTLTVDTAYGGDSFVIVDAKSLGFEIIPDEARDLAETGAKITAAANEQIKFRHPVQQD
                     WNKISFCQFTLPVEIKDGILSGRNTVAIDPGKLDRSPCGTGCSARMAVMHSRGEMQVG
                     DHFTATSIIDSRFDCTIRESTSIGETKAIIPTIRGRGWITGTHQLMCDPEDPWPSGYR
                     LTDTWPKQKINK"
     assembly_gap    1425..1524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1526..3568)
                     /locus_tag="EYO68_10500"
     CDS             complement(1526..3568)
                     /locus_tag="EYO68_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC08270.1"
                     /translation="MTIIQSTKMAFETLLSPLKVGSITLKNRVMMGSMHTGLEEHPDG
                     SSRLAAFYAERARGGVGLIVTGGFAPNAAGRVFEEGAELVSEDQLDFHQPIVEAVHEN
                     GGKIVLQILHTGRYGLLENCVAPSPITAPINFYRPKELTPDDILQTIEDYARCAKLAQ
                     KAGYDGVEIMGSEGYLINQFIAKCTNKRSDDWGGSYENRIRFPLRIVERVREESGEDF
                     LIIFRLSCLDLVEDGSSFEEVVELGQRVEEAGASLINTGIGWHEARIPTIAMMVPRAA
                     FAWLTGRLRPHLNIPIVTSNRINDPYVAESLLRDGLADMVSMARPFLADENFVNKTAE
                     GRPEEINTCIACNQACLDHIFKMETATCLVNPRAGHETELNYETASIPKSIAVIGAGP
                     AGMTAAYISAMRGHRVTLFDRRPELGGQINLAVKIPGKQEFFETLRFYRVMLEKYGVE
                     LKLGQTVDAETLNSGKFDLVVLATGVKPRELELDGIDHPKVLSYLDVLDKERVVGQKV
                     AVIGAGGIGIDVAHFLTAQTPFSGEVPEYLQGYGILDSEKALALRKPKKREVTVLQRS
                     ADKIGKRLGKTTGWAHIQTLKSHGVQLLPGATYLKIDDSGLHVRVALKKGEEPKDIVF
                     EVDNVIVAAGQEPRRELETSLRKAGFEVHVIGGAKAALGLDAKTAISDGAELAAKL"
     gene            complement(3645..>3969)
                     /locus_tag="EYO68_10505"
     CDS             complement(3645..>3969)
                     /locus_tag="EYO68_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIC08271.1"
                     /translation="PTGLVVICQNEKSQLKNKNQALKVLRARLYEKELEAQQSATAEQ
                     RRGMVGSGDRSERIRTYNFPQGRLTDHRINLTLYKLEEILLGKLEQVIDPLLSHHQAE
                     MLKAS"
BASE COUNT          999 a          955 c          808 g         1107 t
ORIGIN      
        1 ctgagcgggt gattctggta acagacgcta cagccggagc agcggcgcct caaggacgtt
       61 acaagttagg aaattctgaa ttgcagcgtg aaacggaacc cgtcatttta gacccgcaaa
      121 catcccgacc cgtaggttct gctgtaacgc tggatcagtg tgtgcgtaac gtaattaact
      181 ggtatgagat gcctttgaaa gaagccgtaa gctgggcagc cgaaaatccg ctgcaattgc
      241 tcaactcagc aaaagttaaa actcaattat cagcacggga gcaaaccgtt tggtggaaag
      301 aagaggaaga ggagtggcag gtaaaagccg ctcggagcgg aaagtttttg tttgagaatt
      361 gataatcagc aattttgata ctatttatta attttctgtt tcggccatgt atctgtcaga
      421 cgatatccag aaggccatgg atcctccgga tcacacatta actgatgtgt acctgttatc
      481 caaccccgcc cgcgaattgt cggaataatc gccttcgttt caccaatact tgtactttcc
      541 ctgattgtgc agtcaaacct tgagtcaata attgaggttg cagtaaaatg atctcccacc
      601 tgcatttctc ccctggagtg cattactgcc attctcgcag aacaaccagt tccgcaaggt
      661 gatctgtcca acttccctgg atcaatagca accgtgtttc ggccgctaag aattccatct
      721 ttaatttcaa caggcagcgt aaactggcaa aaagaaattt tgttccaatc ctgctgcact
      781 ggatgtcgaa attttatttg ctcattggct gcagcagtta ttttggctcc agtttccgca
      841 aggtcacggg cttcatccgg aattatttca aaccccagtg actttgcgtc cacaatcaca
      901 aaactgtcac ccccgtaagc ggtgtccaca gtaagagttc ccaatccgga cacctccagt
      961 tttacatcca gtttgtccgc aaacgaagct acatttttaa tttcaatact cacagcctta
     1021 ccgttttcac attgtgcttt gacctgcaat aatcctgctg gagcttcgag ataaaaagag
     1081 gtttcaggtt cctccatctg aatcattcca ctgtccagca aaactgtggc aacacaaatt
     1141 gaatttgacc cggacatggg aggcgtgtgt tccggttcca tgataatgaa accgaaatcc
     1201 gctttttcac ttttaggggg aaccaataaa tttacatgtt tgaatactcc gccccgtggt
     1261 tcattcaaaa cgaagcggcg caaagtctga tcttgctcaa tccagcggga ttgttcccaa
     1321 atactgtctc ccggcggtgg attcactcca cccactataa catcaccgac ttctccctca
     1381 gcatgacagg aaacgatatg tattatttta gttgatctca ttttnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnttcaaa gttttgccgc caattctgca ccatcgctga
     1561 ttgcggtttt tgcgtcaagt ccgagcgcag ctttggctcc gccgattacg tgtacctcga
     1621 atccagcttt gcgtaaagaa gtttccaatt cccgtcttgg ctcctgtcct gcggcaacaa
     1681 tcacattgtc cacttcaaac acaatatctt ttggttcttc accttttttc aaagcaactc
     1741 gcacatgcag tccggaatcg tcgattttaa gataagttgc acccggaagg agttgtacac
     1801 cgtgtgattt taaagtttgg atatgtgccc agcctgtagt ttttccaagc cgtttgccta
     1861 ttttgtctgc agaacgctgt aaaaccgtaa cttcacgttt tttaggtttc cggagagcca
     1921 aggctttttc cgaatcaaga atcccgtagc cctgtaaata ctccggaact tctccggaaa
     1981 aaggtgtttg tgcagtcaaa aaatgcgcga catcgatacc gattcctcct gctccgatta
     2041 cagcaacttt ttgtccaact actcgttcct tgtctaaaac atccagataa ctcagaactt
     2101 taggatgatc gattccatcc agttccaatt ctcgaggctt tactccggtt gccaacacaa
     2161 caagatcaaa ttttcctgaa ttaagagttt cggcatcaac agtctgcccc agctttaatt
     2221 ccactccata cttttccagc attactcgat agaagcggag tgtttcaaaa aattcctgtt
     2281 tcccgggaat tttcactgct aagttgattt gaccgccaag ttcaggtcgc cggtcaaaca
     2341 aagtgacccg gtgtccacgc atggcagaaa tgtaggcagc cgtcattccg gctggacctg
     2401 ctccaatcac tgcaatagat tttggaatgc ttgcagtttc gtagtttaat tcggtttcat
     2461 gtcctgcacg cggattaact aaacaagttg ctgtttccat tttaaaaata tgatccagac
     2521 aagcctggtt gcaggcaata caagtgttga tttcttccgg acgtccctct gcagttttgt
     2581 tgacaaaatt ttcatcagcc aaaaatggac gggccatcga gaccatgtcc gccagcccgt
     2641 ctcgcaagag actctctgcc acatacggat cgtttatgcg gttggaagtc acgatcggaa
     2701 tatttagatg aggacgcaac ctgcctgtta gccaggcaaa tgctgcacgg ggaaccatca
     2761 tggcaattgt cggaatccgt gcttcatgcc agccgattcc ggtattgatc agcgacgctc
     2821 ctgcctcttc tacacgctgt ccaagttcaa caacttcctc aaaactgctt ccgtcttcaa
     2881 ctaaatcaag acaagaaagg cggaatataa tcaagaaatc ttctccggat tcttcccgaa
     2941 cccgctccac aatccggagt ggaaacctga tccggttttc gtaagaaccc ccccaatcgt
     3001 cgctgcgttt atttgtacat ttggcaatga actggttgat cagatagcct tcagagccca
     3061 ttatttccac gccgtcataa ccggcttttt gtgccagttt tgcgcagcgt gcataatctt
     3121 cgatagtttg taaaatatca tccggagtta attctttggg acggtaaaaa tttattggtg
     3181 cagttattgg ggatggcgca acgcaatttt ccaggagccc gtaacgccct gtatgtaaaa
     3241 tctgcaaaac tattttgcca ccgttttcat gaactgcctc tacaatcggt tggtgaaaat
     3301 ccaattgatc ttccgaaact aattctgcac cttcttcaaa aacacgacct gctgcatttg
     3361 gcgcaaagcc cccggttaca atgagtccca cgcctcccct ggccctttct gcataaaaag
     3421 ccgccagacg agatgaacca tcaggatgtt cttcaagtcc tgtatgcatt gaacccatca
     3481 taactctgtt tttcagggta atgctgccga ctttcagtgg actaagcagg gtttcaaaag
     3541 ccattttagt ggattggatt atggttatgt gttttgggtt ttatctacaa acaaatcaag
     3601 cttataaaga agcgcttaac tttatactgt aaactggtaa atactcaaga tgctttgagc
     3661 atttctgcct gatggtgaga caacaagggg tcaatcacct gttccagctt gcctagcaaa
     3721 atttcttcaa gtttgtatag agtgagattg atgcgatgat cagtaagacg accctgaggg
     3781 aaattatagg tccggatacg ttcgctcctg tcaccgcttc caaccatgcc tcggcgttgt
     3841 tctgcggttg cagattgttg ggcttcaagt tccttttcat agagccttgc ccgcaggacc
     3901 tttaaggctt ggtttttatt ttttaactgg gatttttcat tttgacaaat gacaaccagt
     3961 ccggtagga
//
LOCUS       DTVD01000287            3958 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8342_length_3958_cov_165.892390, whole genome
            shotgun sequence.
ACCESSION   DTVD01000287 DTVD01000000
VERSION     DTVD01000287.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_3958_cov_165.892390"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            347..604
                     /locus_tag="EYO68_10510"
     CDS             347..604
                     /locus_tag="EYO68_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08272.1"
                     /translation="MNICIVSTYSCTFDDFSALVKESEETFREFITEYEVVKVNDHKS
                     IMMCHCTDMEAFGEMMSSPEMKEWDKENNCVDIVYSLEKIN"
     gene            complement(976..2340)
                     /locus_tag="EYO68_10515"
     CDS             complement(976..2340)
                     /locus_tag="EYO68_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HIC08273.1"
                     /translation="MNTKFVEEAESFLEQHPDIREFDLLLPDMNAVFRGKRIQREKLI
                     SIYKKGFYFPASVFAMDVTGETMEETGLGFKSGDQDIPCYPIPETLKVVPWRKERGQL
                     LLRMLEPDGSPFFGNPREVLHSVCKKLSEFGWKAMVAVEIEFYLVDPKRRPGQPPLPV
                     FSRLTGQRETEGQLYSLSSLDAHELFLKDISEAAIIQNIPADTALAELAPGQFEVNLH
                     HVPDAVLACDHAVLLKRIIRSAAQNHQVEATFMPKPYPDQPGNGMHVHLSLLDKEGQN
                     VFQGSDDSGSPELRHAVEGIRSVMAESMLICAPHANSYHRFQSGSYAPVNMSWGYNNR
                     TVSIRIPANNGPDMRIEHRLAGADANPYLLVSALLAGLHYGLITKTEPPAPVEGNAYL
                     QTDTELPLTWEQARNHFDHAKILPEYFGKEFCLLFSNLKLGEQIRFMSTIAPLEYDWY
                     LKTV"
     gene            2751..>3958
                     /locus_tag="EYO68_10520"
     CDS             2751..>3958
                     /locus_tag="EYO68_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010171010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIC08274.1"
                     /translation="MKRDPRFDILFEPVKIGPVTSKNRFFQVPHCNGMGYLRPKSLAA
                     MRGIKAEGGWGVVCTEEVYIHSSSDCDPLVEGRLWDEGDIPALAKMTEAVHEHGSLAG
                     IELQHGGYCSTNHYSRIPPISPSPISVESYAPIQARAMDKSDIRNFRKWHRQAALRSK
                     QAGFDLVYVYAAHSGTLLTHFLSRRINRRTDEYGGSLENRVRLFRELIEDTKDAVGDS
                     CAVAVRFCVEEFLGAQGITSENEGKEIVEMLAELPDLWDVNISDWSMDSATSRFESEG
                     YQEPYMAFVKSMTSKPVVGVGRFTSPDTMLSLIQRGVMDMIGAARPSIADPFLPKKIE
                     EGREDEIRECIGCNICVMSDELSVPISCTQNPTMGEEWRRGWHPERIEPSNSDDGVLV
                     VGGGPAGLECA"
BASE COUNT         1151 a          779 c          897 g         1131 t
ORIGIN      
        1 gatttaataa ggaccactga taggtttcct gtcggttgtt tctggaaaac ccaatttata
       61 ccatcaatcg gtttgtttta caagaaatag ggattttact tggagagtgt ctgactgtga
      121 agaattagtg ggagttactc acaggagatg atggggttgt cgttcgccat ctgagcaagc
      181 atctaggcag aaatgtgact aactacttta tgtactaatt atgtcctgct gatcacacgg
      241 gaaaagttgc atttactacg ttccaaaaaa agtgttttaa taataaatta gctgtgatac
      301 cattctcggg acacgtttaa catataactt aattaggaga gatcaaatga acatttgtat
      361 tgtgtccact tacagttgca cttttgatga tttttcagct cttgtcaaag aaagtgaaga
      421 aacatttaga gaatttatta cagagtatga ggtggtcaaa gtaaatgacc ataaatcaat
      481 tatgatgtgc cactgcaccg acatggaagc ctttggagaa atgatgtcat caccagagat
      541 gaaagagtgg gacaaagaaa ataactgcgt tgatattgtc tactcactcg aaaaaatcaa
      601 ttagttctga aagggggcat gtcaaaacag gaaattagta aaaagaataa aatctttttc
      661 atcttcacct caataccaaa ctaaaatcct aatggtggtg aggagaaccc aaaatataac
      721 atcaatcggt ttgtttccaa gaattgggga ttttacttgg agggtgtcag actgtgaaga
      781 aatagtgggg gtaactcaca tgtgatgatg ggttggtttt acaagaattg gagattgatc
      841 catttctttc ataaatttcc cctatgatcc tgtcagggca agttcaccag taaagtattt
      901 acactgcact gaaaatgaat tgaaaattag gtttcatgtt ataagtttta tataatatca
      961 attgccgctg tcaagctaga ctgttttcaa ataccagtca tattcaagtg gagcgattgt
     1021 actcataaaa cgaatttgct cacccagctt caaattactg aatagtagac aaaattcttt
     1081 tccaaaatat tcaggtaaaa ttttggcatg atcaaaatga tttctggctt gctcccaggt
     1141 tagcggaagt tctgtatcag tctgcaagta tgcgttccct tcaaccggag caggtggttc
     1201 agtttttgtg attaatccat aatgtaaacc agcaagcaaa gctgacacca gcaaatatgg
     1261 attcgcatct gctccggcaa gccgatgctc aatcctcatg tcaggaccat tattggctgg
     1321 aatcctgata gatactgtac ggttgttata gccccatgac atattgactg gagcataaga
     1381 accgctttgg aaacgatgat aagaattagc atgcggtgca caaattagca tgctttcagc
     1441 catcacactt cgtattccct ccacagcatg tcgcaactct ggacttcctg aatcatcact
     1501 gccctgaaag acattttgtc cttccttatc cagcagactc agatgaacat gcattccatt
     1561 tccgggctga tcaggatagg gtttgggcat gaaggtggct tccacctggt gattttgagc
     1621 cgcacttcgg atgatgcgtt tgagtagtac tgcgtgatca catgccagaa ctgcatccgg
     1681 aacatggtgt aaatttacct caaattgtcc tggagcaagt tctgcaagtg cggtgtctgc
     1741 aggtatattt tgtataattg cagcttctga aatatctttt aaaaataatt catgagcatc
     1801 caaactgctt aaagaataga gttgaccttc tgtttcccgt tgtcctgtta agcgggagaa
     1861 aacaggtagt ggtggttgtc cgggtcgtcg ttttggatcg actaaataaa actcaatttc
     1921 aaccgcaacc attgctttcc agccgaattc ggacaatttt ttgcaaacag aatgaagcac
     1981 ttcacgtgga ttcccaaaaa atggagaacc gtctggctca agcattctaa gcagtaattg
     2041 cccgcgttct tttcgccagg gtaccacttt cagagtttcc ggaatcggat agcagggaat
     2101 atcctgatcg ccgcttttaa aaccaagtcc ggtttcctcc atcgtctccc cagtgacatc
     2161 catggcaaaa acagaagctg ggaaatagaa tccttttttg taaatgctaa tgagtttttc
     2221 acgttgaatc cgttttcctc tgaacacagc gttcatgtct ggaagcaaca aatcaaactc
     2281 acgaatatca ggatgctgtt caagaaacga ttctgcttct tcaacaaatt tggtattcat
     2341 tcttccaact cactcgtatg ataatgatga aaaaggagaa tgggaattac caacaaggcc
     2401 tgcatgataa ttgtcactgc cgaagccacc agccaatctc gattgatgaa acactccttt
     2461 caaagtatct tcccaaccat caaagtttcc ggaccgtaaa gcaattcagg aatgaaaaat
     2521 tctcctatcg atggaatgaa taacagcata gcattttttt aactgttagt ccttcaatga
     2581 ttttttatac tctgtcaagt agtagaattg ggacttgtgg tctctgtact cggatgttat
     2641 tcctattatt caagcatgct atggcagaga tttaagaggc agcttgaatt taatgagaag
     2701 ttgattcccg acaaattatt atagaaaact aattatcaaa caactgttaa atgaagagag
     2761 atcctcgttt cgatatcctt tttgaacctg ttaaaatcgg gcctgtaaca tcaaagaatc
     2821 gtttttttca agttccacac tgcaacggga tgggatattt gcgtccaaag agtttggctg
     2881 cgatgcgagg aataaaggct gaaggtggtt ggggcgtagt ctgtacggag gaagtataca
     2941 tccattccag ctcagattgc gatccgctgg tagaaggtcg tctctgggac gaaggtgata
     3001 tcccagctct agcaaaaatg actgaggctg ttcatgaaca tggttcgttg gcaggtatag
     3061 aattacagca tggcggctac tgcagtacaa atcattatag tcgaataccg ccgatttctc
     3121 caagcccgat ttctgtcgaa tcctatgctc caattcaggc ccgtgcaatg gacaagtcgg
     3181 atatacgcaa ctttcggaaa tggcatcgtc aggcagccct gagatccaag caggcaggtt
     3241 tcgatcttgt ttacgtttat gccgcgcata gtggaacact gttgacacat ttcctctctc
     3301 gccgcatcaa caggcgcacg gatgagtacg gaggtagttt ggaaaacagg gtgcggctgt
     3361 ttcgtgagtt gatcgaagac accaaagatg ctgtggggga tagttgtgca gttgctgtgc
     3421 gtttctgtgt tgaggagttt ttaggagcgc aggggataac ttctgaaaat gaaggtaaag
     3481 aaatcgtgga aatgctggca gaactgccgg atctttggga tgttaacatc agcgactgga
     3541 gcatggattc tgctacttca cgtttcgaaa gtgaaggata tcaagaacca tatatggcct
     3601 ttgtcaaatc aatgacatca aagcctgtgg tgggagtcgg tcgttttact tctccagaca
     3661 caatgctctc tttgatccag cgtggtgtta tggacatgat aggagcagca cgcccttcca
     3721 ttgcagatcc cttcctgcca aaaaaaattg aagaaggtcg cgaagatgaa atccgggaat
     3781 gcattggctg taatatctgt gtcatgagtg acgaactatc agtgccaatt agctgtacgc
     3841 aaaacccaac tatgggtgag gaatggagac gtggttggca tccggagcgt attgaaccga
     3901 gtaatagtga cgacggtgtg ctggtggttg gtggcgggcc tgccggactg gaatgtgc
//
LOCUS       DTVD01000288            3957 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8349_length_3957_cov_380.687852, whole genome
            shotgun sequence.
ACCESSION   DTVD01000288 DTVD01000000
VERSION     DTVD01000288.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8349_length_3957_cov_380.687852"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="EYO68_10525"
     CDS             complement(<1..494)
                     /locus_tag="EYO68_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIC08275.1"
                     /translation="MKETTATEKLSQVLDTIPKLLLDHAKKRADRPANRLKDLGIWQT
                     WTWSEVADEVRNLACGLSKIGFQRGDKLAVIGDNRPRLYWSMVAAQALGGIPVPLYQD
                     SVAGEMVYVLENADVKYAVVQNQEQTDKLLEIKDRLPNLKHICYEEPRGMRDYTQDFI
                     HYYK"
     gene            737..1720
                     /gene="phnD"
                     /locus_tag="EYO68_10530"
     CDS             737..1720
                     /gene="phnD"
                     /locus_tag="EYO68_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC08276.1"
                     /translation="MKALLKKLILSTSLLGLLALPALAECPRGDLDKRYCDVNGDLVA
                     DAPTDSSKWLDPDTLIFAYTPVEDPAVYRGVWADFLDHMEKVTGKKVVFFPVQSNAAQ
                     IEAMRSGRLHVAGFNTGSNPLAVNCAGFVSFTIMASLNGHFGYEMEIITYPGSGINKV
                     EDIKGKQLAFTSPTSNSGFKAPSALLKSEFGMIQDRDFTATFSGKHDNSIMGVAHKDY
                     AAASIANSVKFRMISRNVIKESDVKTIYKSQTFPTTGFGHVYNLKPSLAEKVKESFFS
                     FKWDKADGTPTSLKVEFSKSNEGQFLPITYKEFWAVIRTIDKANNVSYACK"
     gene            1805..2632
                     /gene="phnC"
                     /locus_tag="EYO68_10535"
     CDS             1805..2632
                     /gene="phnC"
                     /locus_tag="EYO68_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIC08277.1"
                     /translation="MLEIKQLVKKYHTGDLAINGVDLKVEKGQVMALIGPSGAGKSTL
                     IRCVNRLENPTSGEIWLNGENIVKMRSGKLRRARRNMGMIFQEYALVERLTVMENVLS
                     GRLGYVGFWRSFLRKFPQSDINAAFGLLEKVGLDTMVNKRADELSGGQRQRVGIARAL
                     IQKPDILLVDEPTASLDPKTSRQIMRLITELCEENQLTAIINIHDVALAQMYAERIVG
                     LREGSIVYDGSPDDLKPDVLTEIYGKEDWTAVRKRKPKKKKSIEENPTTKEHMEEMQ"
     gene            2723..3481
                     /gene="phnE"
                     /locus_tag="EYO68_10540"
     CDS             2723..3481
                     /gene="phnE"
                     /locus_tag="EYO68_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIC08278.1"
                     /translation="MKNPLLRWGLLLGLILYLFLSIGSLDVNWHRIYVGLDRGAKFVY
                     GFMNPNFAGRWTDISEGMIESLVMTVTSTAIGIAISIPIGFGAARNLAPLPVYLFCRG
                     IIALSRSFQEIIVAILFVAIFGFGPLAGVLTLSFATIGFLSKLLAEDIESIDKSQAEA
                     VQATGSAWWQWLNYGIQPQVMPRLIGLSLYRLDINFRESAVVGLVGAGGIGATLNTAF
                     DRYEYDTAAAILILIIGIVMLSEYISGYIRAGVQ"
     gene            3481..>3957
                     /locus_tag="EYO68_10545"
     CDS             3481..>3957
                     /locus_tag="EYO68_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIC08279.1"
                     /translation="MAETLSSGTKVWQYRSRNKQLLSWFIWLIGTALFMYCWQRVSES
                     TTWFFVWDAPRIAADIGSRMIPPRWSYINELWVPLWDTLNIATIGTMMAIVMAVPVAF
                     LAARNTTPSKFVRPIALLIIVSSRSINSLIWSLLLVSIIGPGVVAGIVAISLRSIGF"
BASE COUNT         1050 a          881 c          891 g         1135 t
ORIGIN      
        1 tctttgtagt agtgtataaa atcctgggtg tagtcacgca ttccgcgggg ttcctcgtag
       61 catatatgct tgaggttcgg caagcgatct tttatttcga gcagtttgtc agtctgttcc
      121 tggttctgga caactgcata cttaacgtct gcattttcca gcacatatac catctcccct
      181 gcaaccgagt cctgataaag cggaacggga attccgccca gtgcctgtgc ggcaaccatc
      241 gaccagtaaa gacggggacg attatcacca atcacggcta gtttgtcccc tctttgaaaa
      301 ccaatttttg aaagtccaca agccagattc cgaacttcat ccgccacttc agaccacgtc
      361 catgtctgcc agatgcctaa atccttaagg cggtttgcgg gcctgtcagc tcttttcttt
      421 gcatgatcca acaacaactt tggaattgtg tcaagaactt ggcttaattt ttcagtagct
      481 gtcgtttctt tcatttaatt tcaggtagtt tccttaactg ttttattgaa acagtggatg
      541 ttgaatcttt tcagtaataa tgtcaagtca tttttctcca gtgaaaaatt tttttcacta
      601 agaaattttg ttttcattaa aaaagtgttg acgattgttt caaatcagtt taatctacat
      661 actttttcaa gtccggcgtt gtctgtcaat tctgtcgaca tgccgttttt ttaactcata
      721 ataaggatga agcactatga aagcactatt aaagaaactt atcctgagca cttctttgct
      781 tgggttgctg gcgttaccgg ctctggcaga atgtcctcgc ggtgatttgg acaagcgata
      841 ctgcgatgta aacggagatt tggtggctga tgctccaaca gattccagca aatggctgga
      901 tccggacacc ttgatatttg cctacactcc cgtcgaagac cctgctgttt acagaggtgt
      961 ttgggcggat ttccttgatc acatggaaaa agtgaccggg aagaaagtag tattttttcc
     1021 tgtgcagtca aatgcagcac aaattgaagc catgcgttcc ggccgtttac atgttgccgg
     1081 attcaacact ggttccaatc cgctggccgt caactgtgcc gggtttgttt cttttacgat
     1141 catggcaagt ctcaacggac attttggtta tgaaatggaa atcataacct atcctggtag
     1201 cggcataaac aaggtggaag acatcaaagg caagcagctt gctttcactt ctcctacatc
     1261 aaattcagga ttcaaagcac catccgcatt gcttaaaagc gagtttggca tgatccagga
     1321 tcgtgatttc acagcaactt tttctggtaa acacgataac tccataatgg gtgtggcaca
     1381 caaggattat gctgctgcct cgatcgcaaa ctctgtaaaa ttccggatga ttagccgtaa
     1441 cgtgatcaaa gagagtgatg tgaaaaccat ttacaaatcc caaactttcc ctactacagg
     1501 tttcggacat gtttacaact tgaaaccgtc actggcagag aaggttaagg aatcattttt
     1561 tagtttcaaa tgggataagg ctgacggcac acccacttcg ttaaaagtgg agttctcaaa
     1621 atctaatgag ggccaattcc ttccgattac atacaaggaa ttctgggcag ttattcgaac
     1681 catcgataaa gcaaataatg tttcttacgc ttgcaaataa cctcctcatc ggttccaact
     1741 gaagcaccgt cactgtgctt tcttagttgg agccgaacac gcttccgcgt gctgcaaaat
     1801 atctatgctt gaaataaaac aactagttaa aaaatatcat accggagacc ttgccataaa
     1861 tggtgtggat cttaaagttg aaaagggcca ggtaatggct cttattggac catccggtgc
     1921 aggaaaaagc acattgatcc gctgtgttaa ccggctggag aatccaacat ccggcgaaat
     1981 ctggctcaat ggtgaaaata tcgttaaaat gcgttcggga aagttacggc gcgcccgcag
     2041 gaacatgggt atgatttttc aggaatatgc cctcgtagaa cgtcttactg ttatggaaaa
     2101 tgtactttcc ggacgcttgg gatatgttgg attctggaga agttttttga ggaagtttcc
     2161 gcaatcagat atcaatgcag ccttcggtct cctggaaaaa gttggacttg atacaatggt
     2221 aaataaacgc gccgatgaac tatccggagg tcaacgccaa agagtcggta ttgccagggc
     2281 gttgattcaa aaacccgata ttttacttgt tgacgaaccg actgccagtc ttgatccaaa
     2341 aacttctcgc cagataatgc gtctcatcac agaactgtgt gaagaaaatc aacttacggc
     2401 aatcatcaat atccatgatg ttgcactggc gcaaatgtat gcagagagaa tagttggttt
     2461 acgtgagggc agcatcgtat atgacggctc tccggatgac ttaaaaccag atgttctaac
     2521 tgagatctat ggaaaagaag actggactgc tgtccgcaaa cgaaagccta aaaaaaagaa
     2581 gtccatagaa gaaaatccaa ctacaaaaga acacatggaa gaaatgcagt aaatgactaa
     2641 cattcccgca aacccgccag accccggagt ttccggacag cagacatcaa cttttccggc
     2701 aaattggagt cctccgccat ttatcaaaaa cccgctttta agatgggggc ttctgcttgg
     2761 tctcatactc tacctgttcc tgtctatcgg ttcacttgat gtgaattggc accgaattta
     2821 tgtgggtctt gaccgcggag caaaatttgt ctatggattc atgaatccaa acttcgctgg
     2881 acgatggaca gacattagcg aaggcatgat tgaaagcctg gttatgactg tcacttcaac
     2941 tgcaattgga atcgcaattt ccattccaat cggttttggt gcagcccgca accttgcacc
     3001 cttaccggta tatttgtttt gcaggggaat cattgcactt tccagaagtt ttcaggaaat
     3061 aattgttgct atactgtttg tggccatttt tggttttggt ccgcttgccg gtgtattgac
     3121 tctttcattt gcgaccatcg gttttctttc caaacttctt gcagaagata tcgaaagtat
     3181 tgacaaaagc caggctgaag ctgttcaggc aacaggctcc gcctggtggc agtggctcaa
     3241 ttatgggatt cagccgcagg tgatgccgcg tttgattgga ctttcgctgt atcgacttga
     3301 tatcaatttt cgtgaatctg cggtagtcgg actggttggc gcaggaggaa taggcgcgac
     3361 actcaacacg gcatttgacc gttatgaata cgacactgcc gcagcaattc ttattttgat
     3421 cataggaatt gtcatgctct cggaatatat ttcaggatat atccgtgcag gagtgcagtg
     3481 atggccgaaa cactttcctc cggaacaaaa gtctggcaat acagaagccg caataaacaa
     3541 cttctttcct ggtttatctg gctgattgga accgcgcttt ttatgtattg ctggcaaagg
     3601 gtttctgaaa gtacaacctg gtttttcgtt tgggatgccc cgagaattgc ggctgacatc
     3661 ggttcgcgca tgatacctcc gcgctggagt tacatcaacg aattgtgggt accgctctgg
     3721 gacaccctca acattgccac catcggtaca atgatggcaa tcgtaatggc tgttccggta
     3781 gctttcctgg cagcacggaa cacaacgccc agcaaatttg tgcggccgat tgctctcctg
     3841 atcatagttt cttcacgttc aatcaactct ttgatttggt cacttttgct ggtgtctatc
     3901 atcggtccgg gagtggtcgc gggaatcgtt gccataagtt tgcgctccat tggtttc
//
LOCUS       DTVD01000289            3954 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8356_length_3954_cov_434.949218, whole genome
            shotgun sequence.
ACCESSION   DTVD01000289 DTVD01000000
VERSION     DTVD01000289.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_3954_cov_434.949218"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(31..1449)
                     /locus_tag="EYO68_10550"
     CDS             complement(31..1449)
                     /locus_tag="EYO68_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIC08280.1"
                     /translation="MPTDARVIRVDAKIDQWVYAGDTLAVLKDSKGAKFKFRAGVSGQ
                     LVFWRLRNLKHYGAGETVGILRIAPVIVEEVGSGTSVVALPAFNEMLLNLYNSTGLAS
                     LFRGQGLDWTEGFGRLIMIGVGLILLYLGIRRKFEPLLLVPIGFGAMLSNIPLAGLSE
                     PGGLLYYVYEFGITTGIFPLIIFMGVGAMTDFGPMLANPKTALLGGAAQFGIFGTLLG
                     ALALNGIPGIDFSLRDAASIGIIGGADGPTAIFLASQLSPRLLGSIAIAAYSYMALVP
                     IIQPPIMRLLTSKQEREIEMTQLRYVSTREKIIFPLVALSLCALLLPSAAPLIGMFMF
                     GNLAKECGVINRLSDTIQKSLINTVTIFLGLSIGSKLAAGEFLNIETVGILILGMIAF
                     SVGTATGVLMAKLMNNLSSAPINPLIGAAGVSAVPMAARVVNQVGLEANSQNHLLMHA
                     MGPNVSGVIGSAVAAGVLLAML"
     gene            complement(1613..3409)
                     /locus_tag="EYO68_10555"
     CDS             complement(1613..3409)
                     /locus_tag="EYO68_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HIC08281.1"
                     /translation="MARKRIEFMDTSFRDGFQSVFGARVATKDFLAPLEAAVDAGTTY
                     FEAGGGARFQSLFFYCNESAFDMMDAFRKTVGPEANLQTLARGINVVALSQQPRDMID
                     LHAKMFKKHGITTIRNFDALNDLRNLKYSGECISNHGLHHQIVIAMMDLPPGCEGAHD
                     AEYYLKTLRNILDSDVPFDSICFKDASGTANPRKVHETIKGARKMVPEGTILHLHTHD
                     TAGASVSQYLGAIEGGIDRIDLAMSPVSGGTGQPDILTMWHALKGTDYTLDIDPDKII
                     KAEEVFAESFEDYFFPLESRMVSPLIPFSPMPGGALTANTMMMRDTGTLHLFPLVIKE
                     MSEVVRLGGFGTSVTPVSQFYFQQAYLNVTLGKWEKINPGYGNMVLGYFGRTPVEPDP
                     EIVRLASEQLGKPIFKDDPLDVLEPGMPKAAEALKKNNLPETEENLFIASSCEAKGID
                     FLLGKAKISIRKKSDEAEKKAPTSAKSAAPSVSGPRDYTITVDGRAYQVQVNAGGTVA
                     AADDSGNTPVSAPTATQTSGIDIPAPTPGNIVRLEVEVGDTIAKEQTLLVMEAMKMES
                     EVKSPQAGVVQAVHVQAGNTVQTGDALLTLRG"
     gene            complement(3454..3711)
                     /locus_tag="EYO68_10560"
     CDS             complement(3454..3711)
                     /locus_tag="EYO68_10560"
                     /inference="COORDINATES: protein motif:HMM:PF04277.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08282.1"
                     /translation="MLWEGLKLMGIGMATVMLFLLLMIVCIEWVKHLTRNYTLLQQQV
                     LKSQRKSRTNSPETDTTSAVPIEVFAAAISTFESDNNSTKT"
     gene            3842..>3954
                     /locus_tag="EYO68_10565"
     CDS             3842..>3954
                     /locus_tag="EYO68_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIC08283.1"
                     /translation="MYLNQEEIEKFEQDGFLVLKDFVSQDACEALSHRATE"
BASE COUNT         1094 a          957 c          862 g         1041 t
ORIGIN      
        1 aaactaaacg acactgaaac cacatgcaaa tcatagcatt gccaacaaaa ctccggctgc
       61 taccgcggag cctatcactc cggaaacatt tggccccatg gcgtgcatca gcaggtggtt
      121 ttgtgaattg gcttcgagcc caacctgatt cacaacacgt gctgccatgg gaactgcaga
      181 aactcctgca gccccgatca aaggattgat tggcgcagat gacagattat tcatgagttt
      241 ggccatcaac acacccgttg ctgttcctac tgaaaatgcg atcatgccaa gaatgagaat
      301 tcccactgtc tcaatattaa gaaattctcc ggcagctaat ttgcttccta tgctaagtcc
      361 aagaaatatg gtcacagtat tgatcagtga tttttgaatg gtgtctgaaa gacgatttat
      421 cacaccgcat tcttttgcca ggttaccaaa catgaacatg cctatcaatg gggctgcaga
      481 aggcaacaaa agtgcacaca aactaagtgc aactaagggg aaaataattt tttccctggt
      541 tgaaacataa cgcagctggg tcatttcgat ctcacgctct tgctttgaag tcagcaaacg
      601 catgatcggg ggttggatga tgggtaccag cgccatatac gaataggccg caatggcgat
      661 tgatccaagt aaccgtggtg aaagctggct ggcaagaaaa atagccgttg gaccgtctgc
      721 accaccaatg atgcctatgg aagctgcatc ccggagagaa aagtcaatgc cgggaattcc
      781 gttcaatgcc agcgcaccca agagggttcc aaaaatcccg aactgtgccg cgccgcccaa
      841 caaagcggtt tttggattgg ccagcatcgg cccgaaatct gtcattgcac caactcccat
      901 gaaaattatc aaaggaaaaa ttccggtggt tataccaaac tcgtaaacat aatacaagag
      961 cccgcccggc tccgaaagac cggctaaagg gatattggac aacatagcgc caaagccgat
     1021 aggcaccagc agcaacggtt caaatttccg gcgaatgccc aaataaagca agatgagtcc
     1081 cacaccgatc atgatcaacc gtccaaatcc ttcggtccaa tccaatccct gtccacgaaa
     1141 cagtgacgcc agtccggtag aattgtaaag gttgagcaac atttcattaa aagcaggtaa
     1201 agccacgaca gacgtaccac tccccacttc ctcaacaatt accggagcaa tcctaagaat
     1261 tcccactgtt tctccggctc cgtaatgttt cagatttcgc aaccgccaaa acaccagttg
     1321 tccggaaact ccagcacgaa acttaaattt tgcgcctttt gaatctttga gtactgccaa
     1381 tgtatctccg gcatagaccc attgatcgat tttcgcgtca acccgaatga cccgtgcatc
     1441 ggtgggcatc acgattttga tttggtcctc accggactgg ccaagcaaag ttcctgacag
     1501 tcccaaacaa aacaataacg caaaaacctg taccagtttt ttatttattt ttcgaaaaat
     1561 actcaggtgg aataaaagga gcagatccac ctttatactg tctcgcatcg tctcaccctc
     1621 tcaatgtgag taaagcatca ccagtttgca ccgtatttcc ggcttgtaca tgtacagctt
     1681 ggacgactcc agcttgtggc gatttaactt cggattccat tttcatggct tccatcacca
     1741 acagcgtttg ctctttagca atggtgtcgc caacctccac ttccagtcgc acgatgtttc
     1801 ctggcgtggg tgctgggata tcgattccgg aagtttgcgt agcggtcgga gcagagaccg
     1861 gtgtattccc agaatcatca gccgcagcca ccgttccacc ggcattgact tgaacctgat
     1921 aggctcggcc gtcgacggtg atggtgtaat ctcttggacc ggaaacagaa ggtgctgctg
     1981 attttgcgga agtcggtgct tttttttctg cttcatctga ttttttccgg attgaaattt
     2041 ttgctttgcc cagcagaaaa tcaatgcctt tggcctcgca actggaagca ataaaaaggt
     2101 tttcttcggt ttcagggaga ttgttttttt tgagggcctc ggctgctttt ggcattcccg
     2161 gttccagcac gtcgagcgga tcgtctttga aaatcggttt gccgagttgc tctgatgcca
     2221 gcctgacaat ttccggatcc ggctctaccg gtgtccggcc gaaataaccc agcaccatat
     2281 ttccgtatcc cggatttatt ttttcccatt tgccaagggt cacgtttaga tatgcctgct
     2341 gaaaataaaa ctgtgaaact ggcgtgaccg aagtaccaaa acctccgagc cgaacaactt
     2401 cagacatttc cttaataact agtggaaaaa ggtgcaaagt tccggtatca cgcatcatca
     2461 tagtgttggc cgtcaatgcg cctccgggca tcggagaaaa cggaatcagc ggtgaaacca
     2521 ttcgtgattc cagagggaaa aaataatcct caaaactttc tgcgaacact tcttccgcct
     2581 taatgatttt gtccggatca atgtccagag tgtaatcggt ccctttgaga gcgtgccaca
     2641 tggtgagaat atcgggttgt ccagttccac cgctcaccgg actcatcgcc agatcaatcc
     2701 tgtcgattcc accttcaatc gcacccaagt attgactcac agaagctcct gcagtatcat
     2761 gagtatgcaa gtgcagaata gttccctcag gcaccatttt tcgagcgcct ttgatagttt
     2821 cgtgaacttt acgtggattg gcagtaccac ttgcatcttt gaaacaaatc gagtcaaaag
     2881 gcacgtctga atccagaata ttacgaagag ttttaaggta atattctgcg tcatgtgcac
     2941 cttcacatcc cggaggcaaa tccatcatgg caatcacaat ttggtggtgt aaaccgtgat
     3001 tagagataca ctctccggaa tactttagat tacgtaaatc attcaatgcg tcaaagttcc
     3061 tgattgttgt gatgccgtgt ttcttgaaca tttttgcatg caaatcaatc atgtcccgcg
     3121 gctgctgaga gagtgccacc acgttgattc cgcgtgcaag tgtttgtaga tttgcttctg
     3181 gcccaaccgt tttgcgaaaa gcatccatca tgtcaaatgc cgattcgtta caataaaaaa
     3241 acaaactttg gaaacgtgct cccccgccag cttcaaagta ggttgttccg gcatcaaccg
     3301 ctgcttccag gggggccaga aaatctttgg tagcaacccg tgcaccaaag acggattgaa
     3361 acccatcccg aaaggatgtg tccataaatt caatacgttt cctggccatg atgtttttct
     3421 ttttagagac agttaaaagc taaagcaaat tatttaagtt ttcgtggaat tgttatcgga
     3481 ttcgaatgtt gaaattgctg cagcaaatac ttctattggt acagcagatg ttgtatcagt
     3541 ttcaggggaa tttgtcctgg atttgcgctg cgacttaagt acttgctgtt ggagcagggt
     3601 ataattccgt gtcaggtgtt ttacccattc aatgcacaca atcatcaaca gcaggaaaag
     3661 catgactgtt gccatgccta tgcccatcaa cttgagacct tcccacaaca tatgtcacaa
     3721 tggttgagaa ttaatttaag agtcaatttg tacttgccac aaatcaagat tgaagtattt
     3781 tcgattgact aaatcgagca tcttataata taccaatttc aggggcaaaa tcaaggaagc
     3841 tatgtattta aatcaagagg aaattgaaaa atttgaacag gatggctttt tggtgctgaa
     3901 ggactttgtt tcacaggatg catgtgaagc tttgagtcat cgagcaactg aaat
//
LOCUS       DTVD01000290            3934 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8430_length_3934_cov_238.540088, whole genome
            shotgun sequence.
ACCESSION   DTVD01000290 DTVD01000000
VERSION     DTVD01000290.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8430_length_3934_cov_238.540088"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="EYO68_10570"
     CDS             complement(<1..443)
                     /locus_tag="EYO68_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08284.1"
                     /translation="MAYLSAKNRKLVLLLSVVILAVFPLSITAEEQPLNQQTTAVHYV
                     FTQSVERALYPWLKEEVANTWFESNIPKSCHICDNVYRYPVDNGSVVTRGVQNMMNRK
                     RLSTILEVQEFLTVIVRQSGEILSVEASLHNLEKEEPLWAKVFEWS"
     gene            complement(451..2535)
                     /locus_tag="EYO68_10575"
     CDS             complement(451..2535)
                     /locus_tag="EYO68_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08285.1"
                     /translation="MFFTAPDFWRKREHRSVLSFCWKVIFLLHIFGLSFHGILFAEEK
                     ALLEFSDNDSRGGWLSGDLIITVPDKVIPAKISGKISKFVLHWGNNPHQRLGMFRPIV
                     VLPSAESGSRMRIQFKATRVPSGATHFLLYARLENGEETEKFSLSLIDKGVPQSKPQN
                     ILFKQTGKEGNRVQGEIRITRAWDERDVSHYAVYWGEGADTVLRSQPSVTVIEKKSWF
                     GSLAAQLQAPWMVNILTEEIDVLLPPAATHLIVFSRNADGQMNEGTSVELEGTETKDK
                     NLSQKMLLHKKSAPSGMIAGSVTLFQSKGGTDSEHYLFFWGKDKKTRLEDLPPLTQFE
                     VKKIKDGLTVKEIQVSTLTVMDQKLQVSSENDADRELKFEFPINTFIPEGATYILAFT
                     QQKFWFQDDAERRLKGPIAAVSIQDPDGSRNKEKKKSKIKEGLKNFSITQFKKKSVLS
                     QEKKENTESVFQGEKTKGTKIRKGPDWRISENRGLGLGLSFSGLNGIVTFYDYNLDDN
                     QQLHYSLELTGQQVGSSFKALRLTEESTDMQSIAKSGSGNSLEIDRILVSATYRWFVD
                     ESLVWGISEGLFYGAGGGFGYATLKYQGKDTSTVASISGDTETFDSTATDYYHSANAL
                     GLFAVLDAGWQGLENYYFQIALQPSIYFYYNDNFEESSIPVNPNQRSTVVDLWSRAKN
                     LTRLLLGFGVFF"
     gene            complement(2541..3920)
                     /locus_tag="EYO68_10580"
     CDS             complement(2541..3920)
                     /locus_tag="EYO68_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08286.1"
                     /translation="MTSSVTLNSEGLMIPTITTVSSNAKQIFPEVGAGGTVSQESGKL
                     VVTIPLTTSGSSRNSSKRSSSLRTSQRGGVSSEYATTGQWVGVDSTSGKPVYVQSASA
                     GATLVLENAYDNTTTVSLSAGTADLRIGDALPTALSSTVSVKSTPASVTMSGSRNLVS
                     LPAYTSIAPASFESQFSSYKAVWVRRSDAWQFYTSSDNKTVYTDKGYTELTSSIEAGE
                     GFWVELNAPVNPTNLEVANYGGYTALAQLSSMTSEWNIAGTSKKITVEEITQAANFDQ
                     TSEDESNTLGFIENSGGNGPPSGLQISNFEFEIAVNEFGQSSRIALLMAVLLLASASL
                     MLYRRQTQLRNSSQSQNIFLRIPAWQLFSVAGIIMFVVACAPPQGDSTSSTFDYSGSY
                     DRVHSVWKWDDENSKWLAYSPKTSVAQELSTEGYSTFSFVEKGQGYWVRLSVSGVPTS
                     LSFAEPPAF"
BASE COUNT         1098 a          926 c          754 g         1156 t
ORIGIN      
        1 gaccattcaa aaaccttggc ccagagaggt tcttcctttt caagattgtg cagtgaagct
       61 tcaacactga gtatctcacc agattgtctg acaatcactg tcaaaaattc ctgtacttcc
      121 agaattgtag acagcctttt gcggttcatc atattctgaa caccacgtgt taccacagaa
      181 ccattgtcta ctggatagcg gtagacattg tcacagatgt gacatgattt tggaatatta
      241 ctttcaaacc aggtatttgc cacttcttcc tttaaccagg gatacagagc ccgctcaaca
      301 ctttgagtaa aaacatagtg tactgctgta gtttgctgat tcaacggctg ttcttctgct
      361 gttatcgaca acggaaaaac agctaaaata actactgaca gcagcaacac taacttcctg
      421 ttttttgcag ataagtaagc catagttatc ttaaaaaaac acaccaaatc ctaatagtaa
      481 acgtgtaaga ttctttgctc ttgaccagag atctacaact gttgagcgtt gattgggatt
      541 aaccggaatg cttgattcct caaaattgtc attataataa aaatatatcg acggctgaag
      601 cgcaatttga aaatagtagt tttccagtcc ctgccaacct gcatcaagta ctgcaaaaag
      661 tccaagtgca tttgcagagt gataataatc ggtggcagtt gagtcaaagg tttcagtatc
      721 accggatatg ctggctaccg tacttgtgtc tttaccctga tatttcaaag ttgcatagcc
      781 gaaacctcct cccgcaccat aaaataatcc ctctgaaatt ccccaaacta gagattcatc
      841 gacaaaccag cggtaagttg cagaaactaa aattcggtca atttcaagag agttgccgga
      901 tccgcttttt gcaatactct gcatatctgt actctcttct gtcaaacgca gagctttgaa
      961 ggagcttcca acttgctgac ccgtgagttc cagagaataa tgcaattgct gattatcgtc
     1021 caaattataa tcgtaaaaag tgactatccc attcaatccg gaaaaagata gtcccaatcc
     1081 caaaccacga ttttcagaaa tcctccaatc cggacccttc ctgattttcg tcccttttgt
     1141 tttttctccc tgaaatacac tttcagtatt ttcttttttt tcttggctta atacactttt
     1201 tttcttaaat tgtgtaatcg aaaagttttt aaggccctct tttattttac ttttcttttt
     1261 ttctttattc ctgcttccat ctggatcctg tatcgacaca gctgcaatcg gcccttttag
     1321 cctgcgttca gcatcatcct gaaaccaaaa tttttgttga gtaaaggcca gaatgtatgt
     1381 cgcaccttca ggaataaaag tgtttatcgg aaactcaaat tttaactcac gatctgcgtc
     1441 attctctgac gatacctgca gtttttgatc catcacagta agagtggaaa cttgtatttc
     1501 ttttacagtc agtccatcct tgattttctt tacttcaaac tgagtaagtg gcggcaaatc
     1561 ttccagtctg gtttttttat cttttcccca aaaaaacaaa taatgctccg agtcagtccc
     1621 acctttgctc tggaaaagag taacgctacc ggcaatcatt ccggaaggag cagatttttt
     1681 atgcagcagc attttttggg aaagattttt atcttttgtc tccgttcctt ccagttcaac
     1741 agaagtacct tcattcattt gcccatctgc attcctgctg aaaacaatca gatgagtggc
     1801 cgcaggaggc aaaagaacat ctatttcctc tgtcaatatg tttaccatcc agggagcttg
     1861 gagttgggct gctaaactac caaaccagga ttttttttca attacggtaa cagacggctg
     1921 cgatcttaaa actgtatccg caccttcacc ccagtacacg gcataatgac tcacgtcccg
     1981 ctcatcccag gcacgtgtta tgcggatttc accttgaact cgattacctt cctttcctgt
     2041 ttgtttaaat aatatattct ggggcttact ctgcggaact cctttgtcaa tcaaactaag
     2101 actgaacttt tcagtttctt ctccattttc aaggcgggca tagagtaaaa agtgagttgc
     2161 gcctgaagga actcgggtgg ctttaaactg gattcgcatc cggcttccgg actcagctga
     2221 cggcagtacc acaatcggcc ggaacatacc cagacgctga tgaggattat ttccccaatg
     2281 cagtacaaat ttagatattt tcccggaaat tttggctgga atgactttgt ctggaacagt
     2341 gataatcaaa tctccactga gccagcctcc acgtgaatcg ttgtcactga actcaagcaa
     2401 tgccttttct tccgcaaaca agatcccgtg gaaactcaga ccgaaaatgt gcagcaaaaa
     2461 tatgactttc cagcaaaatg aaagcactga cctgtgttca cgctttctcc aaaagtccgg
     2521 agcagtgaaa aacatatagc tcaaaatgca ggaggttctg caaatgagag actggtagga
     2581 acgccgctga cactaagacg tacccaatat ccctggcctt tctctacgaa actgaaagta
     2641 ctgtagccct cagttgacaa ttcctgagca acagaagttt ttggtgagta tgccagccat
     2701 ttgctgttct catcatccca cttccaaact gagtggacac ggtcatacga tccggaatag
     2761 tcaaaagttg aagatgttga atcaccctgc ggtggagcac atgccacaac aaacataatt
     2821 atgccagcta cagaaaacaa ctgccaggca ggaatccgta gaaaaatgtt ttgagactgt
     2881 gaactgtttc taagctgagt ttgtcttcgg tacaacatca aacttgcaga agcaagcagc
     2941 agcactgcca tcaaaagcgc aatccgtgaa gattgtccaa actcgtttac tgcaatttca
     3001 aattcgaaat tcgaaatttg caaccctgag ggcggcccgt ttcctccgga attttcaata
     3061 aaacccaaag tattggattc atcttctgaa gtttgatcaa aatttgcggc ctgagttatt
     3121 tcttcaaccg tgatcttttt tgaggttcca gcaatattcc attctgaagt catactgctt
     3181 aattgtgcca atgcggtata tccgccataa ttagccactt ccagatttgt tggattaacc
     3241 ggtgcattca attcaaccca aaatccttcc ccggcttcaa tgcttgacgt taactccgta
     3301 tatcctttat cagtgtagac agttttattg tcactgcttg tataaaattg ccatgcatca
     3361 ctgcggcgta cccagacagc tttgtaactg ctaaattggc tttcaaaact tgctggagca
     3421 atcgaggtgt aagccggcag tgaaaccaga tttcgtgaac cgctcattgt tacggaagcc
     3481 ggtgtacttt ttacagatac agtactgctc aaagcagttg gcagtgcgtc gccaatacgc
     3541 aggtcggcgg ttccagcaga caagctcacg gtagttgtat tgtcatacgc attttccagg
     3601 acgagagttg cacctgctga tgctgactgg acataaaccg gtttgcctga agttgagtca
     3661 acaccaaccc actgcccggt cgtcgcatat tcagaggaaa ctccgccacg ttgtgaagta
     3721 cgtaacgaag aacttctttt tgagctattt cgtgaacttc ctgaagtagt caaaggtatt
     3781 gtaacaacca acttacctga ttcctgactg acggttccgc ctgccccaac ttcagggaaa
     3841 atctgcttcg cattcgagga tacagtggta attgttggaa tcatcaagcc ttcactattt
     3901 aaggtaacac tgctggtcat actggtactt ggcg
//
LOCUS       DTVD01000291            3933 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8434_length_3933_cov_315.512893, whole genome
            shotgun sequence.
ACCESSION   DTVD01000291 DTVD01000000
VERSION     DTVD01000291.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8434_length_3933_cov_315.512893"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="EYO68_10585"
     CDS             complement(<1..1973)
                     /locus_tag="EYO68_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIC08287.1"
                     /translation="MKIKTEFPHKIQTIKHIWIPMPDGCRLAARIWLPENAEQQPVPA
                     ILEYIPYRKNDGTALRDSLYHPYFAGHGYAAMRVDMRGSGDSEGVMLDEYLQQELDDA
                     VSVIAWLVEQPWCDGSVGMMGKSWGGFNSLQVAALQPPELKAIITVCSADDRYADDVH
                     YKGGCVLTTDMLGWSTTMLAWNARPPDPDVLGENWRKRWLERLEQTPLLVRQWLNHPH
                     RDDYWKHGSICEDFSKVQCAVYAVGGWADPYSNAIPRMLSGLTCPRKALIGPWAHEYP
                     QRALPGPQIGFVQECLRWWDYWLKDIENGIMEEPQLRAWMPDSIAPSTHYEERPGRWV
                     AEAKWPPKNDESQTWYLNRNGLNQNAETDKPMLFPTSQTVGLNAGVWFPMGAAGEFPG
                     DQRSADSGSLCFTSEAFYDGMEILGNPEATVTLSADQTEALLAARLCDIAPDGSSLLV
                     SWGLLNLTHRNGHENPEALDPGEKFTAKVQLNACAHALKSGHRWRLSLSTAYFPHAWP
                     SSKITKLKIFPGIETYLNLPFRKIPQEDTQLAEFLEPECSQPLATEEIRTASRKRTIE
                     HEVIEDSFTLTHRVDNGRFRFVEDGLETEDLSCDSLSLNEDKPLSLKVCCERTAGIGR
                     GDWQTRVEASGTMTATSENFHVTSRLEVFEGDQR"
     gene            complement(2072..2836)
                     /locus_tag="EYO68_10590"
     CDS             complement(2072..2836)
                     /locus_tag="EYO68_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIC08288.1"
                     /translation="MQIEWQEWIGLSPVMFLYAVVAIGVAAFIRGYSGFGFSALTVTS
                     LSLILPPAEVVPSAFMLEIAASIYMLPLVWRTVNWKVLRWLLLGVVLGTPFGITVLTT
                     VPQKPMQLLISVIVLAASLLLWKKSQTSQTVNRGWTFGVGCISGLINGAAAIGGLPVV
                     LLFLFISAGSASTRASMVAYLFITDLYAVFLPGSQQLISTELLGRTVLLLIPLFLGIY
                     IGHRSFVKTSPESFRKFVLSLLIVLSLAGMIRGILL"
     gene            complement(2851..3762)
                     /gene="gcvA"
                     /locus_tag="EYO68_10595"
     CDS             complement(2851..3762)
                     /gene="gcvA"
                     /locus_tag="EYO68_10595"
                     /inference="COORDINATES: protein motif:HMM:PRK11139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HIC08289.1"
                     /translation="MDSFNGFPSLNFLHTFESVARHLSFTNAAKELFVTQAAVSHQIK
                     ALEDYLGVKLFHREKRKVFLSEEGQKLLPSVVSGLQGIADSLENIRNYDEEDTIVVGV
                     GSSFSANWLVHRLGAFYQKYPEVNLHLKISNNDPDFVADGTDLAVVWGKGDWQGLMCE
                     QLMVVEFTPVCSPELLNKTHPLKTPEDLIHYPLLDDPDYNIWQEWLEEAGIPERKYKR
                     RTVIRDSNVLIHSTLDGHGIALCAVGIVQEYLDSGRLIRPFDLSITGGGFYYLVYPEK
                     ALRKPLVRLFKDWLISEVTKTEFQETS"
BASE COUNT         1115 a          944 c          852 g         1022 t
ORIGIN      
        1 cgctgatcgc cttcaaaaac ttccagcctg gaagtgacat gaaaattttc ggatgtagca
       61 gtcattgtac cagaggcttc gactcgggtt tgccagtcac cacgcccgat ccccgctgta
      121 cgttcacagc atactttcaa agaaagaggt ttgtcttcat ttagagaaag ggaatcacaa
      181 ctaagatctt cagtttccag cccgtcttct acgaaacgaa agcgtccatt gtccactctg
      241 tgagtgagag tgaaactgtc ttcaattacc tcatgctcta tagttctttt gcggctggca
      301 gttctaattt cttctgtagc caatggctga gaacattccg gctctaaaaa ctcagcaagc
      361 tgtgtatctt cctgagggat ttttctgaac ggaaggttga gataagtttc aatgcccgga
      421 aaaattttta gtttggttat tttcgaactg ggccaggcat gagggaagta cgcagtggaa
      481 agagacagcc gccagcggtg cccggatttg agagcatggg cacaggcgtt gagctgaacc
      541 tttgcggtga atttttcgcc gggatccaat gcttccggat tttcgtgtcc gttacggtgt
      601 gtcaaattga gtaagcccca actcaccagt agtgacgaac catccggcgc aatatcacac
      661 agacgtgcag caagcaatgc ttcagtctga tcggcagaca gcgtgaccgt cgcttcagga
      721 tttcccaaaa tttccatccc atcgtaaaag gcttccgaag taaaacacaa cgatccggaa
      781 tctgctgaac gctgatcacc gggaaactct ccagcggcac ccataggaaa ccacactccg
      841 gcatttaatc ccacagtttg tgaggttggg aaaagcatag gtttgtccgt ttccgcattt
      901 tgattcaagc cgttccgatt taaataccag gtttgagatt cgtcgttttt cggaggccat
      961 tttgcttcgg ccacccaacg tccgggacgc tcttcataat gtgtgctggg agcaatacta
     1021 tccggcatcc aagcccgcag ctgtggctcc tccataattc cgttttcaat atccttgagc
     1081 caataatccc accagcgcaa acattcctgt acaaaaccga tttgtggacc aggtaaagct
     1141 cgttgcggat actcgtgcgc ccaaggtcca atcagcgctt tgcgtggaca ggtaagtccg
     1201 gaaagcattc gcggaattgc gttgctgtac gggtcagccc agccgccaac cgcatacacc
     1261 gcacactgca ccttcgaaaa atcctcacaa atcgaaccgt gtttccaata atcgtcacga
     1321 tgcggatggt tgagccattg ccgcaccaac agtggtgttt gctccaaacg ttcgagccag
     1381 cgtttccgcc aattttctcc caacacatcc ggatccggtg gacgggcatt ccaggcaagc
     1441 atggttgtag accaaccgag catatcggta gtcaggacgc agccgccttt gtaatgcaca
     1501 tcgtcggcgt aacggtcatc ggcagagcaa acagtgatga ttgctttcag ttccggaggc
     1561 tgcaatgctg cgacttgcaa tgagttgaaa ccaccccatg atttgcccat cataccgacg
     1621 gatccgtcac accagggctg ctccacaagc caggcgatta cagaaaccgc gtcgtccagt
     1681 tcctgttgca ggtattcatc cagcattact ccctcagagt ccccactgcc gcgcatatca
     1741 accctcatag cagcatatcc gtgtccagca aaatacggat gatagagaga atcacgcaga
     1801 gcagtaccgt cattttttcg atacggaatg tattccaata ttgccggtac aggttgctgt
     1861 tccgcatttt ccggaagcca gattcgggcg gccaaacggc agccgtccgg catgggaatc
     1921 caaatgtgtt ttattgtttg tattttgtgc ggaaattcgg ttttgatttt catacgaacc
     1981 tgaaacgaac ttgagtaaat agtaaaggat ttagtatttt aaatctttta tttaaaccac
     2041 aagtatgaag cattacttca aaaataagca gttaaagcag aataccacgt atcattcctg
     2101 ccaatgacaa cacaatcaat aaactgagga caaatttacg aaatgattca ggagatgttt
     2161 ttacaaaact gcggtgaccg atgtaaatac caagaaacag tggaattaac aacagaactg
     2221 tgcgtcccaa taattcggtt gaaatcagtt gttggctacc tgggagaaaa actgcgtaga
     2281 gatcggtgat gaaaaggtag gcaaccattg aggcccgagt tgacgcggat ccggctgaaa
     2341 tgaaaaggaa aagcaaaaca accggcagcc ctccaatcgc tgcggcacca ttgatcagtc
     2401 ctgatataca accaactcca aaagtccaac ctcgatttac agtttggcta gtctgtgatt
     2461 ttttccaaag cagcaggctt gccgccagta ctattactga aatgagcagc tgcattggtt
     2521 tctgtggaac agttgttaga actgtaattc caaaaggagt ccccagtact actcctaaaa
     2581 gcaaccagcg caaaaccttc cagttaacag ttcgccaaac caggggcagc atgtaaatgc
     2641 tggccgcgat ttcaagcatg aatgctgaag gtactacttc tgcaggaggt aaaatcagtg
     2701 ataaactggt tacggtcagg gccgaaaaac cgaaaccgct gtatcctcga atgaaagccg
     2761 caacgccaat ggcgaccact gcatagagaa acattaccgg actgaggccg atccattcct
     2821 gccattctat ttgcatacct aaaaagactt ttaagaggtt tcctgaaatt cagtttttgt
     2881 gacctcactg atcagccagt ctttgaataa acgcaccagc ggtttgcgta atgctttttc
     2941 aggatagacg agatagtaaa aaccacctcc ggtaattgaa agatcaaagg gacggatcaa
     3001 ccgtcctgaa tccaaatatt cctgcactat tcccaccgcg cagagcgcaa ttccgtggcc
     3061 gtccaaagta gagtggatga gtacattgga atctctgatg acagtacgac gtttatattt
     3121 ccgctccggt atacccgctt cttccaacca ttcctgccat atgttgtaat ccggatcatc
     3181 cagcagcgga tagtgaatca aatcctcagg agttttcaga gggtgtgttt tatttaataa
     3241 ctcaggactg caaaccggtg taaattcaac gaccattaat tgttcgcaca tcaatccctg
     3301 ccagtctcct ttgccccaga ccactgccaa atcagttcca tctgcaacaa aatccggatc
     3361 attgttagaa attttaagat gaagatttac ttccggatac ttctggtaaa acgcgcctaa
     3421 acggtgcaca agccagttgg cagaaaaaga tgaaccaaca ccgaccacta tcgtgtcttc
     3481 ctcatcgtaa ttgcgaatat tttccaaact gtctgcaatt ccctgtaatc cactgactac
     3541 agatggtaat aatttttggc cctcctctga aagaaatact ttgcgttttt cccggtgaaa
     3601 aagtttcacg cctaaatagt cttccaaagc tttaatctga tgactcaccg cggcttgtgt
     3661 cacaaagagt tctttagccg cgtttgtaaa actcaagtga cgggcaacgg actcaaaagt
     3721 gtgaagaaaa ttaagtgatg gaaatccatt aaaactatcc ataaatattc ctaatcttaa
     3781 tcattaaaaa agatcaattg taattaatgt tcattacttg taaaacaact acttaagggt
     3841 atcaagagaa aaataatatt attgttgttt gattagtatt attgttgatt gattagtatt
     3901 attgttgttt tgattagtac agataatttg taa
//
LOCUS       DTVD01000292           14564 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_851_length_14564_cov_304.960783, whole genome
            shotgun sequence.
ACCESSION   DTVD01000292 DTVD01000000
VERSION     DTVD01000292.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_851_length_14564_cov_304.960783"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="EYO68_10600"
     CDS             <1..765
                     /locus_tag="EYO68_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08290.1"
                     /translation="GNVEELEKFERSGLFDLIVAGSSNDDELNQVMVMQTGSGSFPMI
                     PTKGQGLLTGKLTKAGKLIPDNQEIVPADLSVTWLRPNFEDAPELEGTFRTYDAAVKE
                     LFFSNLDRMEQQRQESPFVGNAVCVGCHANAAAIWKNSRHAHAFATLENKGKHFDPEC
                     LECHVVGLKPWVAPTNASESVLKFAGGTGFLSSQLTPHLKNVQCENCHGPARAHLENS
                     KIHPANKEPKSACVSCHQGSHSPMFNFETYWPKIKH"
     gene            complement(784..1656)
                     /locus_tag="EYO68_10605"
     CDS             complement(784..1656)
                     /locus_tag="EYO68_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIC08291.1"
                     /translation="MSAPGFRQFDTLAVVGLGLIGGSFVMDVRRLGLAKRILGYDNNS
                     EYRAEISSRNLVDYLAEAPDAKLAEAQLVMLAVPVKAFAEVLSNIRPHLSASVILTDT
                     GSVKSPLLKLMRTPVYTGIRFVGGHPIAGSENFGPLAAKENLFSGKRCILTPEMDTDR
                     DVVRTVCEMWESMGSMVSEMDAEDHDQMFASVSHLPHLLAYASIQAIADSSTPEALSH
                     SGAGLKDFSRIASSSPEMWADIFLENQENLLSRMNTFKDVVAKLEDAIKRNDKEKLIE
                     LLAQAKTERDRWMT"
     gene            complement(1664..2596)
                     /gene="secF"
                     /locus_tag="EYO68_10610"
     CDS             complement(1664..2596)
                     /gene="secF"
                     /locus_tag="EYO68_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIC08292.1"
                     /translation="MQFLNFKDGQFIDFLGYKRLAAYISGILILAGVASIVAHNGLKY
                     GIDFRGGTNVQIQFSTPPNLDQLRNFFAQNGMKNVVLQTFGDLADHEILLGLPINSPL
                     GTGENLTSELRKILEPRHPKLEIRRIETVGPKVGDELKISAVKAILIALGLVLLYITI
                     RFQWRQGVSAIVALLHDVIVVVGMFSILDKEFSLTVVAALLTVVGYSLNDTIVVFDRI
                     RENQGKYRKKSFEETINLSITETLSRTLITSGTTLFVVLSLFLLGGEIIHDFAFALLV
                     GVLIGTYSSIYVASPLVVFLTKLAPPGGTVLKSS"
     gene            complement(2635..4203)
                     /gene="secD"
                     /locus_tag="EYO68_10615"
     CDS             complement(2635..4203)
                     /gene="secD"
                     /locus_tag="EYO68_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIC08293.1"
                     /translation="MDIKLKGSIILIVLLGALYYAFPTYRAYQTGVDPQTVDNKVNLG
                     LDLQGGMYLDIEIKVDEAVKEILRRTALELEDLLIDEQVDFVEVRQATNSLILEMEPG
                     KKVNLEIAPYDRFLEQFDMNVQDDLTYLKLKSGEAQLIRENSVSQALEVLRNRIDSLG
                     ISEPSLQKQGEDNIVIQLPGLKDRDRAIELIGPQAILQFQIVNNNATPSTYNRLTEVV
                     KYEEVWDKTTNKLISKRPYVLDKKILMTGEFIRDARVRIDSRDNRPYVALSFDSIGAD
                     RFAKITRRNVGRNMAIVLDDKVQSAPVIREAITGGEASISGQFTVEEADTLKIVLRSG
                     SLPAPIEIREERTVGASLGEDSVDQGLKSLMIGSALVLIFMIFYYRLAGVFAAAALIF
                     NVLLIISVLGAFGATLTLPGMAGIVLTIGMAVDANVLIFQRIREEIKRTDNPRAAIQE
                     GFGKAFRTILDANVTTLFAALALLQFGTGPIKGFAVTLSVGIIASMFTAIVVTRFFFD
                     FVYLRKTKLRSLSI"
     gene            complement(4277..4639)
                     /gene="yajC"
                     /locus_tag="EYO68_10620"
     CDS             complement(4277..4639)
                     /gene="yajC"
                     /locus_tag="EYO68_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIC08294.1"
                     /translation="MFPFESVAYAQSAGEASSQSPFFQFIPLVLILGVFWFLIIRPQQ
                     KKQKAHQRMVDSLNKGDKVVTNGGIFGTIVKVGDDRITLEIASKVQINIERQQVSRMD
                     KKSGVNKDEKEDDSKEKK"
     assembly_gap    4736..4835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5501..6832)
                     /locus_tag="EYO68_10625"
     CDS             complement(5501..6832)
                     /locus_tag="EYO68_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC08295.1"
                     /translation="MAAKIISTQPKNDNSCGWIKQLPPRQSRPYLSGEQHADWVVLGA
                     GYTGLAAARQLSILHPQSRIILLEGQNAGEGSSARNSGFLVDSILNEGHFSASNLEEY
                     RKKYDIKHAGVEAVQRLVAELDIDCDLEANGKIHATAVKDHEKKLLNFSKLLSELELK
                     HKILEGEELHQRLGSSYYCMGLWTEGGILLQPAKLARGMVENLPEQVELYENAPVLNW
                     RKRSHTEGYQLYTPSGQVTCQKLIVTVNSFMASCGVKSNRTFPLTLTASMTRPLTEKE
                     DESIGRPAPWGVLSAQSMGATVRLTKDRRILMRNTVEAWPAINMKFTDLKTRKAKHLE
                     GLKRRFPRLGGDLFESTWSGTICISGNNAHVFTEVEYGMYAAGCYNASGIGLAVLFGT
                     EIANLASGNMTDSIALIQTNSKPMLLPPQPFLRWGVGLRLLRDRFLARHEQ"
     gene            complement(6891..7583)
                     /locus_tag="EYO68_10630"
     CDS             complement(6891..7583)
                     /locus_tag="EYO68_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC08296.1"
                     /translation="MDYNVIIDNLPLYLNGLWVTIQLVVIALVSGFGLAVPLALMAVS
                     KTSLLRYPAKAFIYFFRGTPLLVQMFLLYYGMGQFEAVRESVLWILFKEAYWCAITAF
                     ALNTAGYTAEILRGAIEQTPFGETEAAQACGMSKSTMYRRIILPGSFRRALPAYGNEV
                     IFMLHGSALAGVITIVDLFGAAKIVNARYFVPFESFITAGFFYLCCTFCIVWFFKWVE
                     KHWYAHLRPRIS"
     gene            complement(7587..8273)
                     /locus_tag="EYO68_10635"
     CDS             complement(7587..8273)
                     /locus_tag="EYO68_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIC08297.1"
                     /translation="MFDLHGYFPMILEGMTLTIEVALLSLLIAVILGLFGAIAKLSKS
                     RIARSIATVYTTLIRGIPDLVLMTIIFYGGQIIVNNIGDSLGWDYIDISPFVAGTLTI
                     GFIFGAYMTETFRGGILAVSHGEIEAALAFGMPSWKVFLRITLPLMVRHALPGFGNNW
                     MVLAKTTALVSVIGLHDMMYNAGVAGGATRQPFTFYLVVALLFLCITGLSDLGLRWAN
                     RRYSVGVRTV"
     gene            complement(8403..9173)
                     /locus_tag="EYO68_10640"
     CDS             complement(8403..9173)
                     /locus_tag="EYO68_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIC08298.1"
                     /translation="MKKLIAVAVVAMMILGTSVSANEWNKIRIGVEGAYPPFSWVEKD
                     GTLKGFDIDIAMALCEEIGAECVLVPQDWDGIIPALLARKYDAIIASMSITEERKKKV
                     AFSGKYYNTPAKFARKKGSGITISKAGMKGKKVGVQRATTHDNFITAEFGDSVEIKRY
                     GTQDEAYLDAISGRVDLLLADSIAMDDGFLKTDKGKGWEFVGPGYSDPKYFGVGAGIA
                     VRKSDGELAKLFSLAIKVIRSNGVYHMINGKYFAFDVY"
     gene            complement(9258..10037)
                     /locus_tag="EYO68_10645"
     CDS             complement(9258..10037)
                     /locus_tag="EYO68_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC08299.1"
                     /translation="MDNRNTALVVEELHKYFGSEEVLKGISLEASEGDVIAMVGASGS
                     GKSTFLRCINLLEIPTSGKIFVRGELIRMKQDRYGVTVPEDLKQVERIRTRLGMVFQQ
                     FNLWSHMTVLENVIEAPVHVLKMDKIEAIERAEELLHKVGIHDRMNYHPAHLSGGQQQ
                     RVAIARALAMEPDMLLFDEPTSALDPELVGEVLRVIRQLAEEGRTMIVVTHEMDFARD
                     VSSRVVFLHEGSIEEDGPPGEVFNNPSSERFRQFVSKYVEQ"
     assembly_gap    10194..10293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10311..11729)
                     /locus_tag="EYO68_10650"
     CDS             complement(10311..11729)
                     /locus_tag="EYO68_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIC08300.1"
                     /translation="MNSIRAPKFDYIIIGAGPAGMAAAITARAHGLSVLVLDEQPSAG
                     GQIFRNIESLEATRNSEFSKLGQEYEFGSSLVRRFKKCGADYFTDRSVWQIDNNLQIH
                     HVSASASKSNSVISHCQAKRLLIAVGAMERPMPLPGWTIPGVMPCTAVDVLYKSSGLI
                     PDGEVVLAGSGPLLLLIGCRLLDVGVHVSAFLDTSMNGALLKALPLLPNALRSHKYLT
                     RGLSLLWKIRKAGIPFYSGIQNIEALGDSKLEKVRIGKSGKFFDVTADLLLLHNGVVP
                     NVQITRNLGCEHRWYDVQRYWEPIVDDWGNTSVDHVSIAGDCGRVAGAAISEVSGHLA
                     ALETVYQLGVISKVERDNLSQPHRIELARQKHVRPFLDKLFQPSKTWLVPENDKTLAC
                     RCEEVKVSEIRETVRMGIKDPDSLKSMTRCGMGYCQARMCGLTVSEIIAEEKKVSPEE
                     IGYFKVRSPIRPITLGQLAEID"
     gene            complement(11726..12010)
                     /locus_tag="EYO68_10655"
     CDS             complement(11726..12010)
                     /locus_tag="EYO68_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC08301.1"
                     /translation="MFKKQYESHETVSIIFEGKNLDVEIGQTVASALLVGGNMVFRSS
                     VVSGQPRAPYCMMGVCFECLIEIDGIPNQRACMIPVREAMKIKRQQKNKE"
     gene            complement(11991..13118)
                     /locus_tag="EYO68_10660"
     CDS             complement(11991..13118)
                     /locus_tag="EYO68_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIC08302.1"
                     /translation="MIKENAEVVVVGGGVIGVSLAYGMVKKGAKVILIDKVDNRLTAS
                     RGNFGLVWFQGKGKGMPRYVEWCIEAIERWPEFAENLETETGFNLNYEKTGGLEICLG
                     EEEHQERVNFIEEMSQASRSGRYDCEMISRKELQGMLPKIKLGKEVSGASFCSHDGCV
                     NPLNLLKAMNSSFQQKGGSFRPEQSVQKIECSSNDFKIETGTHSFNAEKLVIACGLMT
                     TKLARMVGMKVSVIPERGQILVTERTSRVFNYPTAGIRQNYDGSFMLGSSHEAVGYNI
                     ETSYEVLQNIAKRAIRILPSLKNLQLIRSWAALRVLTPDQKPVYLESDQYPGAHAITS
                     HSGVSLASIHSSILVEWILEERIPLGFTAFHPKRFNVQEAI"
     gene            complement(13119..13700)
                     /locus_tag="EYO68_10665"
     CDS             complement(13119..13700)
                     /locus_tag="EYO68_10665"
                     /inference="COORDINATES: protein motif:HMM:PF03417.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08303.1"
                     /translation="MDKLETLEVSGTPEDIGFAIGQADADSIQKQVLQLAEFRETERT
                     WQDSSYLKMLDAAARSVFPEYVLELEGMARGAQVEYETLLIWNCRGDLPLSDDAIPES
                     AKHSPEGCTTLLYPAAKSSVAVIAHNEDGPPELDGHCCWLSVRQENGSKFSTFHYPGM
                     LPGHTFSVNSHGLVQTINNIRVDDLQSGIPHWC"
     gene            complement(13773..>14564)
                     /locus_tag="EYO68_10670"
     CDS             complement(13773..>14564)
                     /locus_tag="EYO68_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HIC08304.1"
                     /translation="FDQSEIYTHLDPDGFTVEEELRRTKQLGDTPCKSFPIRAYYELH
                     IEQGPILEAENTSIGVVTGGQGAYWTHITLHGQGSHAGTTPMHFRKDPIMGASRIITG
                     LREIVLKQPGAVGTVGRLETKPASINSIPGQVFFTVDTRHPNEEILANINLDLKNLVN
                     SICSEEGLENEFSIIWEAPTINFHEECVTKVRNAAESLGYSHRDIVSGAGHDACQMSH
                     IAPTGMIFIPCEGGLSHDEAENTTPEQVAAGADVLLNSILASANH"
BASE COUNT         4022 a         3414 c         2930 g         3998 t
ORIGIN      
        1 ggaaatgtgg aagagttaga aaaatttgag cgatccggtt tatttgattt gattgttgca
       61 ggaagcagca atgacgatga gttgaatcag gttatggtaa tgcagacagg atctggttct
      121 tttccaatga ttccaacaaa aggacagggg ttattaacag gtaaattgac aaaagcagga
      181 aagttaattc cagataatca agaaattgtt cctgcagatt tatcagtaac ctggctgcgt
      241 ccaaattttg aggatgctcc ggaattggaa ggtactttcc ggacctatga tgcagcagtg
      301 aaggagctgt ttttcagcaa tttggaccga atggaacaac agcgccaaga atctccattt
      361 gtcggaaatg cagtctgtgt cggttgtcat gcgaatgctg ccgcaatttg gaaaaatagt
      421 cgacacgcac atgcttttgc aactctggaa aataagggaa agcattttga tccggaatgt
      481 ctggaatgtc atgtcgttgg actgaaacca tgggtagctc caacaaatgc ttcagaatca
      541 gttctcaaat tcgcgggagg aacaggtttt ctgagttccc agctaacacc gcatttgaaa
      601 aatgtacagt gtgagaattg tcatggtcct gctagagctc acttggaaaa cagtaaaatc
      661 catcctgcga ataaagaacc aaaatcggca tgtgtgagtt gtcaccaggg tagtcattcc
      721 ccaatgttta attttgaaac ttactggcca aagataaaac actgatagct ttgttctcac
      781 aaactaggtc atccagcggt cacgttctgt tttagcctgg gcaagaagct caattaactt
      841 ttctttatca ttcctcttaa ttgcatcctc aagcttagcc acaacatctt tgaatgtatt
      901 catacgagag agcagattct cttgattttc cagaaaaata tctgcccaca tttcaggact
      961 ggatgatgca atacgggaaa aatctttaag acccgcacct gaatgactca gggcctccgg
     1021 agtgctggaa tctgcgattg cctgaatgct tgcgtaagcc agcagatgtg gtaaatgact
     1081 gactgaagca aacatctggt catgatcttc agcatccatt tctgatacca tcgaacccat
     1141 tgattcccac atttcacaaa ctgtgcggac aacatcccga tcagtgtcca tttcaggagt
     1201 aaggatacaa cgcttaccgg aaaataaatt ttcctttgca gcaagtggtc cgaagttctc
     1261 agacccagca atgggatgcc caccgacaaa acgaattcct gtatagaccg gtgtgcgcat
     1321 caacttaaga agaggggatt ttacgctgcc agtgtctgtt agaattactg atgcagaaag
     1381 atggggacga atgtttgaaa gcacttcagc aaaggctttg accggtacag ccaacataac
     1441 cagttgtgct tcggccagtt ttgcgtctgg agcctcagcc agataatcaa ctaaatttct
     1501 gctgctgatt tctgccctat attctgaatt gttgtcataa ccaagaatgc gctttgccaa
     1561 tcctaaacgg cgaacatcca tgacgaatga acctccgatc agtccgagac caactacggc
     1621 caatgtgtca aactgtctga atccgggggc agacatttac aactcagctt gatttgagaa
     1681 cagttccacc cggtggtgct aatttggtca ggaatactac caaaggactt gcgacataaa
     1741 tcgatgagta ggtaccgatt aacacaccaa cgagtagggc aaatgcgaaa tcgtggataa
     1801 tttcgcctcc cagtaaaaat aaagacagca cgacgaacag tgtggttccg gaagtaatca
     1861 gggtcctgct caatgtttcg gtaatgctca aattgatagt ttcttcaaac gacttttttc
     1921 ggtatttacc ctgattttcc ctgatacggt caaaaactac aatggtatca ttgagagagt
     1981 atcctaccac cgtcaacagg gcggcaacga cggtcaaaga aaattctttg tctaaaattg
     2041 agaacattcc tacaacgaca atcacatcat gtaataaagc tactattgca ctgactccct
     2101 gtctccattg aaaacgaatt gtgatgtaaa gcaaaaccaa tccgagcgca attaaaatgg
     2161 cctttacagc actgatttta agttcgtctc cgactttcgg tccaactgtt tcaatccggc
     2221 ggatttcgag ttttggatgt cgcggttcaa gaattttccg taattctgaa gtcaggttct
     2281 cgcctgtacc aagcggactg tttatgggaa gaccaagcag gatttcgtga tcagccaaat
     2341 ctccaaaagt ttgcagaacc acgtttttca tgccattctg agcaaaaaaa ttacgcagct
     2401 gatccagatt tggtggagtc gaaaattgaa tttgcacatt tgttccacct cgaaaatcta
     2461 taccgtattt caaaccattg tgtgcaacaa tcgaagcaac gccggcaaga attagaatac
     2521 cggatatata tgcggctaaa cgtttgtaac caaggaaatc aataaattga ccgtctttaa
     2581 aattaagaaa ctgcatagtc tttctgtaag ctgatctgct gtgttatggt taaatcaaat
     2641 gctaagtgag cgcaatttag tttttcgtaa ataaacaaaa tcaaaaaaga aacgggtcac
     2701 aacaattgct gtaaacatgc ttgcaatgat gcccacagag agtgtgactg caaagccctt
     2761 gatgggacct gttccaaatt gcaggagcgc aagagcggca aaaagagtgg taacgtttgc
     2821 atccagaata gtccggaaag ccttaccaaa gccttcctga atggctgctc gtgggttatc
     2881 agtacgtttg atttcttcac gaatacgctg gaaaatcaaa acatttgcgt caactgccat
     2941 acctatagtc agcacaattc cagccattcc cggtaaggtc agtgttgctc caaaagctcc
     3001 aagaactgaa ataattaata atacattgaa aatcaatgct gctgccgcaa aaactccggc
     3061 cagccgataa taaaaaatca taaaaattaa aacaagtgcg ctgccgatca ttaatgactt
     3121 cagtccctga tctacagaat cttctccaag gctggcgcct acagtccgct cctcacgtat
     3181 ttcaattggt gcaggtaaag atccggaacg caggacgatt ttgagagtgt cagcttcttc
     3241 tactgtaaat tgtccggaaa ttgaagcttc tccgccggta attgcttccc ggatcactgg
     3301 cgcagactgt accttgtcgt ccagtacaat tgccatgttt cttccaacgt tgcggcgtgt
     3361 gatttttgca aaacgatccg ccccgattga atcgaatgaa agtgcaacat agggtctgtt
     3421 gtccctggaa tcaatccgga ctcttgcatc tctgataaac tcgcctgtca ttaatatctt
     3481 tttatctaaa acataaggcc gtttggaaat caatttgttt gttgtcttgt cccaaacctc
     3541 ttcatatttg acgacttcag tcagccggtt gtaggtgctt ggtgtggcat tattgttgac
     3601 aatttgaaac tgcaaaattg cctgtgggcc aatcaattcg attgcacggt ccctgtcttt
     3661 taaccctgga agctggatga caatgttgtc ctcaccctgt ttctgcagag aaggttcact
     3721 aattcccaag ctgtcgatac ggttccgtaa tacttccaat gcctgactca cggagttctc
     3781 ccgtataagc tgggcttccc ctgattttag ttttagataa gtaagatcgt cctgtacatt
     3841 catatcaaac tgctccagga aacgatcgta gggagcaatt tccaaattta cttttttgcc
     3901 gggctccatt tccagaatca gggaatttgt tgcttgacgt acctcaacaa aatccacctg
     3961 ttcgtcaatc agcaaatctt ccagctcaag ggctgttctg cgtaaaattt ccttaaccgc
     4021 ttcatctacc tttatttcaa tatcaaggta cataccgcct tgtaaatcaa gaccaagatt
     4081 taccttgttg tccacagtct gcggatcaac acccgtctgg taggccctgt acgtaggaaa
     4141 cgcataataa agtgctccaa gcaagacaat gagtatgatg gatcctttaa gtttaatgtc
     4201 cataatttct ggttatagat ttattatttt tagggctaac tttttcaatt cttcagcgtt
     4261 ctttattcct tttttgctac tttttttctt tagaatcatc ttctttttca tccttgttta
     4321 caccggattt tttatccatt ctggagactt gctgccgctc gatattgatc tgcaccttac
     4381 ttgcaatttc aagtgtaatc cggtcgtctc ccacttttac tattgttcca aagattccgc
     4441 cgtttgtgac aaccttgtca cccttgttaa gactgtctac cattcgctgg tgtgctttct
     4501 gttttttttg ttgtggacgg ataattagaa accagaaaac tcctaggatt aatacaagtg
     4561 ggataaattg gaaaaatggg ctctgtgaag acgcctctcc cgcggattgg gcatatgcaa
     4621 ctgattcaaa tggaaacatc taattaactc ctttaaaggt ttatgggtaa ataaaacaat
     4681 aaagtatagc acttcattaa aaatatttca cgtctaaatg cgcaatttcc aaaatnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaagt atagcacttc attaaaaata
     4861 tttcacgtct aaatgcgcaa tttccaaaat ttatttatct ctcataaaat gttggaatag
     4921 caaatagtct gatttcaaca cacctcacgg cagttgattt gtaaagggct ttcactcctt
     4981 cggtttccgc agcgtgttgc tgcggaagca tacatctgta atcataaaat tcgtaaaatc
     5041 gagcggcgaa gtggttctca cggttgttgc aggggcttcg cccctacgct ttcacgcctt
     5101 caaaaaaggg aacactactt ttcattaatg aacttgtaat aaaattgtca ttcccgcgga
     5161 agcgggaata caggagtggg gtttatacgg ctgaataaaa cttaaatcat agatgggtta
     5221 ggagaagccc attcctttcc cctgcacggc ctttcgacgg cgcttccctt cgccaccgct
     5281 caggctccgg aaaagttcgc cggggctacc gttccggaat tgttccttgt cattggtttc
     5341 ccccgccaag tatgacgggg ttacagttcc ggaattgctc ccccgccttt gtgctttccc
     5401 gctacggaga gcggggctac cgttcagata aactatcctg taaaattcct atcataaaac
     5461 ttccccgctg ctgaaagtgg ggttatctta aatgaattaa ttattgctca tgccgcgcca
     5521 aaaagcggtc ccgcaggaga cgcaaaccga ctccccagcg aagaaaaggt tgtggaggaa
     5581 gcagcattgg ttttgagtta gtttggatca gcgcaattga gtcggtcatg tttccgctgg
     5641 caaggttggc aatttcggtt ccgaataaaa cggccaaacc aattccgctg gcattataac
     5701 agcctgcagc gtacataccg tattctactt ctgtaaacac atgcgcgtta tttccgctta
     5761 tacaaatcgt acccgaccag gtggattcaa acaaatcacc accaagacgg ggaaaacgcc
     5821 gtttcagtcc ctctaaatgt ttcgctttac gggtctttaa atcggtgaat ttcatattta
     5881 ttgccggcca tgcttcaaca gtgttacgca ttaaaatccg tcgatctttt gttaaacgga
     5941 cagtggctcc cattgattga gcggacaaca ctccccatgg agccggccgg ccaatagatt
     6001 catcttcttt ttcggtcaat gggcgagtca tgctggcagt cagcgtgagg gggaatgtcc
     6061 gattcgattt tacgccacaa ctggccataa aactattcac cgtcacaatc agtttttgac
     6121 aggtcacctg ccccgagggc gtatacaatt ggtagccttc agtatggctc cgttttcgcc
     6181 aatttaagac cggagcattt tcatacaact ccacttgttc cggtaaattt tctaccatac
     6241 cacgtgcgag tttggctggc tgcagcaata tgccgccctc cgtccataag cccatgcagt
     6301 aataagaact gccgagtcgc tgatgcagtt cttcaccttc aagaatttta tgtttcagtt
     6361 ccagctctga caacaacttg ctgaaattca gcagtttttt ctcatggtcc ttaactgctg
     6421 tagcatgaat tttcccattt gcttccagat cgcagtcaat gtccagttca gctaccagcc
     6481 gctgaactgc ttccactccg gcatgtttga tatcatactt tttccgatac tcctcaagat
     6541 tggatgcaga aaaatgacct tcatttagaa tcgaatccac cagaaatccg gaattcctgg
     6601 cgcttgaacc ttcgcctgcg ttttggcctt ctaaaagaat tatgcgtgac tgaggatgca
     6661 aaatcgacaa ttgccgagcc gcggcaagtc ctgtgtaacc cgcaccaagc acaacccaat
     6721 ccgcatgctg ttcaccggaa agataaggcc gactctggcg aggcggcagt tgtttaatcc
     6781 aaccgcaact gttgtcgttt tttggttgtg tagaaatgat cttagccgcc attgtgattt
     6841 tattttctta gtaagatgaa aactctaatt gaggtattgc gcaatacagt tcaggaaatt
     6901 cttggccgca aatgtgcgta ccagtgtttt tcaacccact tgaaaaacca gacaatacaa
     6961 aaagtgcagc acagatagaa gaaccctgct gtgatgaacg actcaaaagg tacaaaatat
     7021 cgggcattca ctatttttgc tgcaccaaac agatctacaa tcgtaatcac tcccgcaaga
     7081 gcactgccat gcagcataaa aatcacttca ttgccgtaag caggtaaagc acgccggaag
     7141 gaaccgggaa ggataatgcg gcggtacatc gtgcttttgg acatgccgca agcctgggct
     7201 gcttcggttt ccccgaatgg cgtttgttca attgcacccc gtaatatttc tgcagtgtac
     7261 cccgcagtat tcagggcaaa agctgtaatg gcacaccaat atgcttcttt gaaaagtatc
     7321 cagagcacac tttcacgaac tgcttcaaac tggcccatgc cgtaatagag caagaacatc
     7381 tgtaccaata aaggtgttcc ccgaaaaaaa tagataaaag ctttagctgg atacctcaat
     7441 aatgaggttt ttgaaactgc catcaaggct aaaggaactg ctaatccaaa tcctgaaact
     7501 agggcgatca cgaccaactg gattgtaacc caaagcccat tcaaataaag cgggagattg
     7561 tcaataatga cattataatc catgattcaa accgtacgta ccccaacact ataacggcga
     7621 ttggcccaac gtaaaccaag gtcagaaagt ccagtgatgc ataaaaacaa cagggcaaca
     7681 accaggtaaa aggtaaaagg ctggcgagtg gcccctccag caacacctgc attgtacatc
     7741 atgtcgtgca atccaatcac tgaaacgaga gcagtcgttt tggccagaac catccagtta
     7801 tttccgaatc caggcaaagc gtggcgcacc atcagtggta atgtaatgcg taaaaatact
     7861 ttccagcttg gcattccaaa ggcaagagcc gcttcaattt ctccatggga cacagccagg
     7921 atgccaccgc gaaaagtttc ggtcatgtat gctccaaaaa taaaacctat tgtgagggtt
     7981 ccggcaacaa aagggctgat gtctatgtag tcccaaccca gtgagtctcc aatattgttg
     8041 acaatgattt gaccaccata gaaaataatg gtcatcagta ccaaatcagg aattccccgg
     8101 atcaaagtag tgtaaacagt tgcaatgctt cgggctatcc tggatttaga gagcttggct
     8161 atagcaccaa ataaaccaag aatgacagca attaaaaggg aaagtaaggc gacttctatg
     8221 gttagcgtca ttccttccag aatcatcggg aaatagccat gtaaatcaaa cattcctgaa
     8281 taatgtgagg gaaaaaaggg aaggccacgg aatcctcgaa ttccgtggca aaataagaga
     8341 aaaattctaa ctttttagag gcatccactg agtattatgg taaacctcag ccaagataaa
     8401 tcttagtaaa cgtcaaaggc aaaatatttg ccgttaatca tatgataaac accattagag
     8461 cgaattacct taatagctaa actgaaaagt ttcgctaatt caccgtcact cttgcgcaca
     8521 gcgataccgg caccaacacc aaaatatttt ggatcagaat agccaggccc tacaaactcc
     8581 caacctttgc ctttatcggt tttcaggaaa ccatcatcca tcgcaattga atctgcaagg
     8641 agcaagtcaa cgcgtcctga aatcgcgtcg agataggctt catcctgagt cccgtagcgc
     8701 ttgatttcaa cgctgtcacc aaactccgcg gtaatgaagt tatcgtgagt agtcgctcgc
     8761 tgaacgccaa cttttttgcc tttcatacct gccttggaaa tcgtgatacc ggagcctttt
     8821 ttgcgtgcaa acttagcggg agtgttgtaa tacttgcccg agaaagcgac tttcttttta
     8881 cgctcttctg tgatcgacat tgatgctatg atcgcatcat atttgcgggc cagcaaagcc
     8941 ggaatgatgc cgtcccagtc ctgtggaact agaacacatt cagcaccaat ttcctcgcat
     9001 agtgccatag cgatatcaat gtcaaatcct ttcagtgtcc cgtccttctc gacccagcta
     9061 aacggagggt aagcaccctc aacaccaata cggattttat tccattcatt tgcggaaaca
     9121 cttgttccca gtatcatcat ggccactacg gccactgcaa ttaacttttt cataagtcac
     9181 tccatttaga aatgattaac aatttgctgg aaaaacttac tataaatttt tccagattaa
     9241 gcctgaatgg cctaatatta ctgctcgaca tattttgaaa caaactgtcg aaaccgttct
     9301 gaggaagggt tattaaacac ttcccctggc ggaccgtctt cttcgatgct gccttcatgc
     9361 agaaagacca ctcttgatga gacatccctg gcgaaatcca tttcgtgcgt taccacaatc
     9421 attgtccgcc cctcttctgc aagctgacgt atcacgcgca aaacctcccc cacaagttcc
     9481 ggatcaagag cagaagtcgg ttcgtcaaaa agaagcatgt ccggttccat agcaagcgct
     9541 cttgcaattg caacccgctg ctgctggcct cctgaaaggt gcgccggatg atagttcatc
     9601 cggtcgtgaa ttccgacctt atgcaacaat tcttcagctc gctcaatggc ttcaattttg
     9661 tccattttga ggacatgcac aggcgcttcg attacatttt ccaaaaccgt catgtgagac
     9721 caaagattga actgctgaaa aaccataccc agccgagtac gaatacgttc gacctgtttt
     9781 agatcttccg gaacagtgac tccatagcgg tcctgtttca tgcgaatcaa ttcaccgcgc
     9841 acaaagattt tcccgctggt tggaatttcc agcaaattta tgcagcgtag aaaagtgctt
     9901 tttccggaac cgctggcacc aaccattgca atcacgtcac cttcactagc ttccagcgaa
     9961 atgcctttga gaacttcttc actgccaaaa tacttatgca gttcttccac caccagtgca
    10021 gtattgcggt tatccaattt ctcctttggg gttaaagaat taaaattgtc ccaaacggaa
    10081 caaatttcat attatgcccg ggcttccgta aaaatcaaag aagttctcac aaaccgacga
    10141 ttcacgctaa ctctcagtaa aataaatgtc aagaaaaatt attaatcaaa gaannnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgactta acagtcctgt tcagtctatt
    10321 tccgcaagtt gtccaagtgt aatcggcctg atgggagagc gaaccttaaa atacccaatc
    10381 tcttctggag aaactttttt ttcttcagca attatctcag agacggtaag accgcacatt
    10441 ctggcctgac aataacccat accacaacgt gtcatagact taaggctgtc tggatctttg
    10501 attcccatcc gaactgtttc tcgaatttca ctcactttca cttcttcaca gcgacaagca
    10561 agggttttat cattttctgg aaccaaccat gtcttactag gctgaaaaag tttgtccaaa
    10621 aaaggacgga catgcttctg cctagccaat tcaatgcggt gaggttgaga aagattgtct
    10681 ctttcaactt ttgaaattac gccaagctga taaacggttt ccaaagcagc taaatggcct
    10741 gaaacttcag atatcgctgc acctgctaca cggccacagt ctcctgcaat tgatacatga
    10801 tccacactgg tattccccca atcatcgact atcggttccc aatacctctg gacatcatac
    10861 cagcggtgtt cacatcctaa atttctggtt atctgtacat tgggaactac cccattatgc
    10921 agaagcaaaa gatcggctgt aacatcaaag aactttcccg atttaccgat acgaaccttt
    10981 tcaagcttcg agtcccccaa agcctcaata ttttgtattc cagaataaaa aggaatacca
    11041 gcttttcgaa ttttccatag caaagataac cccctagtca gatacttatg agatcttaga
    11101 gcatttggta ataacggtaa tgcttttagg agtgccccat tcatgctggt atccagaaat
    11161 gcagagacgt gtactccaac atcaagcagg cgacaaccta tgagcaacaa caaaggaccg
    11221 cttccggcta gtacaacttc cccatctggt attaatccag aactcttgta tagtacgtcc
    11281 actgcagtac aaggcataac tcccgggata gtccaaccag gcagaggcat ggggcgttcc
    11341 atagcgccga ccgcgataag gagacgtttc gcttggcaat gcgaaatgac ggaattactt
    11401 ttagaggcag aagcggaaac atggtgtatc tgcagattat tgtcgatttg ccatacggat
    11461 ctatcagtga agtagtcagc cccacacttt ttgaaccgcc tgaccaagga acttccgaat
    11521 tcatactctt gacctagttt actgaattca gaattacgcg ttgcctccag tgattctatg
    11581 ttacggaaaa tctgacctcc tgcggagggc tgttcatcta gaacaagaac gctgagtcca
    11641 tgagctcgtg cggtgatcgc agctgccatt cctgctggcc ctgcgccaat tataatgtaa
    11701 tcgaatttcg gagctcttat actattcact ctttgttttt ttgttgtctt tttattttca
    11761 ttgcttccct aactggaatc atgcaagccc gctgatttgg aattccgtcg atttcaatca
    11821 ggcattcgaa acatactccc atcatgcaat agggtgccct tggttgtcca ctgacaactg
    11881 aagatctgaa aaccatattc ccgccaacca gtaaagccga cgcaaccgtt tgccctattt
    11941 ccacatcaag gttcttcccc tcgaaaatga tactgacagt ctcatgcgat tcatattgct
    12001 tcttgaacat tgaacctctt aggatgaaag gctgtaaatc ctagtgggat tctttcttca
    12061 agtatccact caacaagaat actcgaatga attgaagcca aactcactcc actgtgacta
    12121 gttattgcat gtgcgcccgg gtactgatcg gattccaaat aaactggctt ttgatcaggt
    12181 gtaaggacac ggagagcggc ccaactacgg atcagttgca aatttttcag acttggtaat
    12241 attcgaatag ctcgcttagc aatgttctgg agaacttcat atgaagtttc tatattgtag
    12301 ccaacagctt catgcgaaga accaagcatg aagctaccat cataattttg tctgattcct
    12361 gcagtaggat aattaaacac tcgggaagta cgttccgtca caagtatttg ccccctttcc
    12421 ggtatgacgg agaccttcat acccaccatc ctggctaatt tggttgtcat caaaccacat
    12481 gcaatgacaa gtttttcggc gttgaaacta tgcgttcccg tttctatctt gaaatcattt
    12541 gaactacatt caattttttg aacagattgt tcgggtctga aactccctcc tttctgctgg
    12601 aatgaactgt tcatagcttt aagaagattt agtgggttta cacatccgtc atgagaacag
    12661 aatgaggctc cactaacctc ctttccaagt tttatttttg ggagcatacc ttgcagctct
    12721 tttctactaa tcatttcaca gtcataacga ccgctccttg atgcctgact catttcttca
    12781 ataaaattga cacgttcttg atgctcttct tctccgagac agatttccaa gcctcctgtt
    12841 ttttcgtagt tcagattaaa accggtctca gtctcaagat tttcagcaaa ctcaggccac
    12901 ctttctatag cttcaataca ccattcaaca taacgcggca tgcccttgcc tttcccctga
    12961 aaccatacca gaccgaagtt tccgcgagat gctgttagtc ggttatcaac cttatcaata
    13021 aggatcactt ttgctccctt ttttaccatt ccataggcta atgaaactcc aataactcca
    13081 cctccaacaa caacaacttc tgcattttct ttaatcattt aacaccaatg gggaattccg
    13141 gattggaggt catcaacccg gatattattg atcgtctgca ccaatccatg cgaatttacg
    13201 gaaaacgtgt gacccggcag cattccggga taatggaaag tggaaaactt tgaaccgttt
    13261 tcctgtctga cactgagcca gcagcaatgt ccatccagtt ccggtggtcc gtcttcattg
    13321 tgggcgatta ctgcaacaga actttttgct gcaggataaa ggagggttgt gcagccttcc
    13381 ggagaatgtt tcgcggattc cggaattgcg tcatcggaca gtggcagatc accacggcag
    13441 ttccagatca agagtgtttc atactcgacc tgtgcgcctc tggccattcc ttcaagttcc
    13501 aaaacgtatt ctggaaaaac acttcgtgcc gccgcgtcaa gcattttcaa atagctgctg
    13561 tcctgccatg ttctctccgt ttcacgaaat tctgcaagct gaagtacttg tttttgaatc
    13621 gaatcggcat cggcttgtcc aatcgcaaaa ccgatgtctt ccggagttcc ggaaacttca
    13681 agtgtttcaa gtttatccac agacagtaac agtttcagaa atttgagtaa gtagaaacgg
    13741 ttggtaaaag ttcatactta tccttttcaa atttagtggt ttgcgcttgc aagaattgaa
    13801 tttagcaata catctgctcc tgcagctact tgttcaggcg tggtgttttc ggcttcatcg
    13861 tggctgagtc ctccttcgca tggaataaat atcatccctg taggggcaat gtggctcatc
    13921 tgacaggcat cgtgacctgc tccggaaacg atatcgcgat gactgtatcc aagagattct
    13981 gcagcattcc tgacttttgt aacacactct tcatgaaaat tgatggttgg ggcttcccag
    14041 ataattgaga actcattctc aagtccttct tctgagcaaa tagagttgac taaatttttc
    14101 aaatccagat ttatgtttgc gaggatttct tcgttaggat gcctggtatc aactgtgaaa
    14161 aagacctgtc cgggaataga gttgatcgat gcaggtttag tctccagtcg tccgactgta
    14221 cctactgctc ccggctgctt cagcacaatt tcacgcagtc ccgttatgat tcgggaagcg
    14281 cccatgatgg gatctttgcg gaagtgcatc ggcgtggtgc ctgcgtggga accttgtcca
    14341 tgcagggtaa tgtgtgtcca gtaagccccc tgaccgccag tgaccacacc aatgcttgtg
    14401 ttctctgcct ccagtattgg cccctgttcg atatgcagtt cataataagc ccggatcgga
    14461 aaactcttgc agggtgtatc acccaactgt tttgtacgtc gtaactcctc ctcaactgta
    14521 aagccgtcag gatccagatg cgtgtagatt tcggactgat caaa
//
LOCUS       DTVD01000293           14546 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_853_length_14546_cov_427.565109, whole genome
            shotgun sequence.
ACCESSION   DTVD01000293 DTVD01000000
VERSION     DTVD01000293.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_853_length_14546_cov_427.565109"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="EYO68_10675"
     CDS             <1..482
                     /locus_tag="EYO68_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIC08305.1"
                     /translation="PQDYLDSRKDVLLEKFKDTAALPGAKEMTTHFFDLGIPQALATS
                     SSSPMFEAKFEKHKKWFSQFTQIVRGDDPELKEGKPAPDIFLLAANRVGVDPAECLVF
                     EDAPTGTEAALAAGMSVVVVPDPNMDHCHYKNASQIISSLKDFDPEYWGLPKFDESI"
     gene            499..1386
                     /locus_tag="EYO68_10680"
     CDS             499..1386
                     /locus_tag="EYO68_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HIC08306.1"
                     /translation="MKVLITGSSGLIGTALSKSLTAGGHSAIRLQRQNNDPDSPVWDP
                     ENCLKDLARVGEIYAVVHLAGENIADGRWSKTKKNRILNSRVKGTKLLAEYFANSNQK
                     PLVIISASAVGFYGDRGAEIVDEDSKAGNGFLANVCVQWEDALKVAVEAGIRVIKVRF
                     GTVLSKKGGALKKMLLPFKLGLGGVFGSGEQYMSWVSIDDAVAMIQYVMTNDSLQGPV
                     NFVTPNALNNRKFTKSLGQVLSRPTIFPLPAFAARIALGEMANELLLSSNRVYPSKLM
                     ESGYKFLHPELNQALINLK"
     gene            complement(1847..4963)
                     /locus_tag="EYO68_10685"
     CDS             complement(1847..4963)
                     /locus_tag="EYO68_10685"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIC08307.1"
                     /translation="MIRWMAEHKVAANLLMVIILVAGVMGVSNIKQEVFPEFDLDFII
                     VAVPYSGATPDEIEESILLPIEDAVSGITGIKKITGTAKESMGSFRIELQKGADKQSV
                     FDDVESAVTRLTTLPEEADDAQVQLPRRRREVLNIALYGDAPARSLIELAQMARDTLL
                     THPDITQVDVEQPRGFEMTLEISKSALQQHALTLNQISGIIRQATLDMPGGKIQTVGG
                     DLLIRTKERRYTAAEYAEIPLLTTDQGILRLGDIAHISDGFEESDLRSRYNGKPSERI
                     LVYRVGEQTPTDVSKAVHEKMEEIKSQLPSSVRTQIVRDSSIILEDRINLLMKNLWLG
                     LGLVFLTLSLFLRVDLSFWIMMGIPISFAGAMISMPITDSSINMISLFAFILVLGIVV
                     DDAIVVGENIYAHQEMKKSPLDAAVDGATEIGPPVLITILTTIAAFIPIYFIPGITGN
                     IFQNIPNVLIVVLLFSLLEAMFILPGHLSHINRVMAWILAPLGKILETPRHYFSSALF
                     WFSNKPYRKILEKGIAYRYTIFALGLFFILLCAGLVAGGHMKFTFFPKIDRDNIAVTA
                     RMPFGTPASVSREVEEKMLRSAEKLLREYEAETGQPVHDGIYSTVGRGGANKTSVRVY
                     LKPLNERKFAAVEFSRRWRKEMGEVAGIEALNIRARHSMGSNYDIDLQLSHPDTNKLL
                     VIVEQFKEKLGEYPGVSNIEDSTEDGKREVQLRLSPAGRTLGLSTQELTQQIRAAFQG
                     VEVFKLLRDSDEVSVKLRLPFEERRYLRDLDDLVILSPTGKLLPLRQVAELKYGQSYS
                     AIRRIDGRRIVSVRALVDSGVGNTGEIQRSIKQELLLKIKSQNPQLQYSFEGAHRAQT
                     NTMDGVKQGGIVALLLIYSLLALQFRSYFQPVIIMTAIPFGMVGALLGHLLMGYSLSV
                     VSVLGIVALTGIVVNDSLILVDFINRSRERGTPIRQAIVEAGVRRFRPILLTTLTTFF
                     GLLPMLFEQSLQARFLIPMAISLAFGVLFSTFVILVLIPVLYMILEDFKGLVSRKPKK
                     VEVKTEVV"
     gene            complement(4960..6168)
                     /locus_tag="EYO68_10690"
     CDS             complement(4960..6168)
                     /locus_tag="EYO68_10690"
                     /inference="COORDINATES: protein motif:HMM:PF13437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIC08308.1"
                     /translation="MWKKLFNLKFWLPVLVLGLGAGVTVALLKNKPKARKKPNFQRGT
                     LVEVMEATLSNPQIEVRTHGRVRAAKKVILSARIGGEVIWIGPKMNEGSFFKKGDPLL
                     SIGIRQSQSRLKAPFNGVVQSKNVDLGQYVNPGTQLAVLLGSDQAEVVIDLPMGRMDW
                     LPQSSTSLDGENQFQFPANVSLAGMISASVWNATVKRHLLELTPRGMMVQLIAEVEDP
                     FRLKRKSKVKSKNKRTSENTLINTVGTANSESPPTPQTSNMPLFVGAFVDVTIPGRQL
                     ENVVNIPAQALRDQDTVWIALEGELEVRRVKIAHLDQDNIYLSGGVKPGEQIIISPIK
                     GAANGLKIRIAGKEKIRKKGDGRRMQLSGERKRRRKRGDGKDEQGDGKWQQRENNQRK
                     QNSESHKEES"
     gene            complement(6182..7630)
                     /locus_tag="EYO68_10695"
     CDS             complement(6182..7630)
                     /locus_tag="EYO68_10695"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00672.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HIC08309.1"
                     /translation="MIQKILVLSKLSGAQDICLLEVKMRLEWLKRPFNSVFTKLIAVM
                     LISGSLLVMGISAISWHGFRENQRNFFRNNLAQYGAYLVRDLEENLNLEHARELSQRL
                     FLIIRYEGENETWETGSGIPPLQKIHFRKWMGKSSDPGFRLLVQPLRPLVTDLRFGRY
                     HRQGVAIVETKTGRFIFAEIKDEWHGIRFALPWIFAVLGLVGLVLTGTWLILRKILQP
                     LNWLSDGVYALGEGNLEHRLPEKRGDELGNLAKAFNRMSERIREMLSAREQLLLDVSH
                     ELRSPLTRMKVALEFIPEDASRESLLDDVQEMEQMVTEILETARLKSEYGQLNLQKTD
                     LVKLIQDICITFEGQTPGIRFENAPETCQTFADAEQIETVLKNLLANALKYSPAENAP
                     VEIRLKKSDAGTLIEIQDHGPGIQDEELDLIFEPFYRTDKSRNRKTGGYGLGLSLCKT
                     IIEAHGGTIDVQSNTGKGAMFSLHLPKSPSTI"
     gene            complement(7551..8261)
                     /locus_tag="EYO68_10700"
     CDS             complement(7551..8261)
                     /locus_tag="EYO68_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIC08310.1"
                     /translation="MTSTTAVSGKILIIDDDEKLNRLLTDYLSKMGFTVLTATLPSAG
                     LEKLEEETPDLVILDVMLPEMDGFEVCRTIRQSSSVPVVMLTARGEVMDRVVGLEIGA
                     DDYLPKPFEPRELVARIQAILRRIQTKSKSGIKTIGALCIDFHKYEVRVDDKLVHLTL
                     NEFECLVLLVKNKGKVLNRDQIIEELRGIEWDAFNRSVDITMSRLRQKLGDDPKNPRF
                     IKTIWGTGYLFIGGENEA"
     gene            complement(8355..9446)
                     /locus_tag="EYO68_10705"
     CDS             complement(8355..9446)
                     /locus_tag="EYO68_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HIC08311.1"
                     /translation="MKKTAIFIALSIVTLVSVSMLLTNYSDNVKYDSNKVHHGKNSFK
                     TKRSASIFQWLSMRFKEGPTPSVAQKDIESILAEVELSQIDLRSASSADVPRATWIGH
                     ATVLVQYQGINFLTDPHLTDYAAPFDFWAKRFTPPALTFAEMPEIDFVVISHNHYDHL
                     DSRTVDMFGDSVTWLVPLGLKAWFLERGIATEKVIELDWWEPHQFSKKGSLTVKITFT
                     PSVHWSKRTPWDTNKSLWGSWAVQIGNFKSWFAGDTAYDEKLFKEIGDKLGPFQLAMI
                     PIGAYGPRYFMLNQHLDPAQAVLVHQEIRSQKSIPIHWGTFQLTHEPFLEPPELLADA
                     MKKTGLPDDEFRAMKIGETIQIKIMVEKR"
     gene            complement(9443..10066)
                     /locus_tag="EYO68_10710"
     CDS             complement(9443..10066)
                     /locus_tag="EYO68_10710"
                     /inference="COORDINATES: protein motif:HMM:PF04264.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIC08312.1"
                     /translation="MKKFFVIITTGILLLLNSTLAFPEEPVFAPEEHCLAYKTVKTMF
                     FFADVDVIGKSCDVTAEMHWEESGEKAQVEVSVPVKTLDSGNPLRDGDIPEILKADLT
                     PNIRFVSEWLEKSAWDKMMEGQLPEVSGNLEVAGGIFPVKFALSFSKQAGFLLVEGQL
                     KTTFSALNVDVPLVAGGLIADPQDELELQLHLRLDKIAGAEKIKNNK"
     gene            complement(10154..10639)
                     /locus_tag="EYO68_10715"
     CDS             complement(10154..10639)
                     /locus_tag="EYO68_10715"
                     /inference="COORDINATES: protein motif:HMM:PF13801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIC08313.1"
                     /translation="MNNRKYRSSYCLRKIILGLLVVVTIPLTLAGCRNKEMSPRRATE
                     LATDRLGLSDEQSQKIAPIAEDLFAEKESLQEIRQTINDEILSQMKSETADAEKLEAV
                     LTGSFEQLRAKLPKFANSFAKFHATLTPEQRAEIVEKMEKRRKRAEKGGWGRHRGGFW
                     H"
     gene            10953..11153
                     /locus_tag="EYO68_10720"
     CDS             10953..11153
                     /locus_tag="EYO68_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08314.1"
                     /translation="MRIAIIVFVGILSYSAVVLPGYFYFVPGGAEEISNLKTLTELPV
                     EDAAKSSTELNIANPGDAPPLK"
     gene            11254..11541
                     /locus_tag="EYO68_10725"
     CDS             11254..11541
                     /locus_tag="EYO68_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08315.1"
                     /translation="MPIHLSFKNIRNSKAVSEHVHQAFEELIKITDDKYPFHVKLNKV
                     ADKSYHVGINCSYRSKQLSSKADHENLYKALSKGVDSMKSQVIRKAEKVRG"
     gene            11599..12111
                     /locus_tag="EYO68_10730"
     CDS             11599..12111
                     /locus_tag="EYO68_10730"
                     /inference="COORDINATES: protein motif:HMM:PF11220.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HIC08316.1"
                     /translation="MFGLVSHHVKYLPAGVLAFAFILLFCVNTQLHAACRGEGFMGVS
                     TNDPIMSWVDITSSPVYSSASTSGTLGCKNWNFSQFLEQSRMQFLQQSHTQLLVETVQ
                     GQGPHLEALAKLMTCPQSSAEAFSNMLWEHRQQTVQIFETAKQTPEFLAELRKWIAAN
                     RQLRNNCSLS"
     gene            12126..12575
                     /locus_tag="EYO68_10735"
     CDS             12126..12575
                     /locus_tag="EYO68_10735"
                     /inference="COORDINATES: protein motif:HMM:PF11220.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HIC08317.1"
                     /translation="MCWKIVLLLAIGISLCSETLWACHKGGPMGFATNDPGGFSLDIT
                     LSPTFTGASTQGTSGCKDWDYAKHQRIQYLETQWTFLSEEASQGKGENLIALAQMMGC
                     AKERQAQFAVLLRHHYFSLFKNTDTRSKFLLKLETLLTQNPSLSCSG"
     gene            12938..13372
                     /locus_tag="EYO68_10740"
     CDS             12938..13372
                     /locus_tag="EYO68_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08318.1"
                     /translation="MNWLKRTVSICALAGSILFGSITSARAETLQIVFNNSLWGAAIG
                     GVSGLAIWALQDEDKEDKLFPQYVVKGMALGMFAGMGVGIFEARSDGGVFMSDGNPKG
                     LLHLDTDLHLLTLRPAKLLPLPEFNAETVSPEWRLDLFTASF"
     gene            13389..14426
                     /gene="rfbB"
                     /locus_tag="EYO68_10745"
     CDS             13389..14426
                     /gene="rfbB"
                     /locus_tag="EYO68_10745"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIC08319.1"
                     /translation="MITGGAGFIGANLVLYWLRHHPDDLLVNFDALTYAGNLGNLEDV
                     KGLPNYHFFHGDLRNALEISKAFSEHNIEAVIHLAAESHVDRSIHVPADFIETNVTGT
                     FNLLETAREFWGDELSKHRILHVSTDEVYGSLGSSGKFLESTPYAPNSPYSSSKAASD
                     HLVRAWHHTYGMNVVTTNCSNNYGPYQFPEKLLPLLINNCTQNLPIPVYGDGKNIRDW
                     LFVEDHCEALDCVFHEGDTGETYNIGGCNEWSNIDIVRYLCRELDKELNRSGADSCEN
                     LITFVKDRPGHDRRYAIDAGKIKNTLNWTPRHTFEEGLTKTIRWYIDHQDWVSNITTG
                     TYRDYYKHQYG"
BASE COUNT         4104 a         3288 c         2879 g         4275 t
ORIGIN      
        1 gtccgcaaga ttacctggac tcaaggaaag acgttttgct ggaaaaattt aaagacaccg
       61 cagccttacc cggtgcaaaa gagatgacga ctcatttttt cgacttgggg attccacaag
      121 cccttgccac cagttcctcg tcgcccatgt ttgaagcaaa atttgaaaaa cataaaaaat
      181 ggttttcgca attcacgcaa attgtcaggg gagatgaccc tgaattgaaa gaaggtaagc
      241 ctgctcctga tatcttcctg cttgctgcaa atcgagttgg ggttgatcct gcggaatgtc
      301 tggtttttga agacgcgcct accggaaccg aggccgcatt ggcggcagga atgtccgtgg
      361 ttgttgtacc tgatcccaat atggatcatt gtcactacaa aaatgcctca caaataatct
      421 cttcactgaa agactttgat ccagaatatt ggggactgcc caaattcgat gaatccattt
      481 gacactggcc cggttctgat aaaagtactt atcacaggat catccggatt aatcggaact
      541 gcattgtcaa agtccctgac tgccggaggt cattctgcga tccgtttgca gcgccagaat
      601 aatgacccgg actcacctgt ttgggatccg gaaaattgtt taaaggattt agcccgggtt
      661 ggggaaatct atgcagtcgt tcatttggca ggtgaaaaca ttgcagacgg tcgttggagt
      721 aaaacaaaga aaaaccgtat cctgaacagc cgggtcaaag gaacaaaact tttggctgag
      781 tattttgcca actctaatca gaaacctctg gtaattattt ctgcgtcagc agtaggtttc
      841 tatggtgaca gaggagcaga aattgttgat gaagacagca aggccggaaa cggtttcctg
      901 gcaaatgttt gtgtacagtg ggaagatgcc ttaaaagttg ctgttgaagc aggaattaga
      961 gtaataaaag tgcgctttgg tactgtactg agtaaaaaag ggggagccct gaaaaagatg
     1021 ctgctgccgt tcaaattggg gctcggcggt gttttcggca gcggggagca atatatgagt
     1081 tgggtcagta ttgatgatgc cgtggcaatg attcaatatg ttatgacaaa cgactcatta
     1141 cagggtccag taaattttgt cacaccgaat gccctcaaca accgtaagtt caccaaatct
     1201 ttaggccagg ttttgagtcg tcccacaata tttccgttac cggcatttgc cgcgcgcatt
     1261 gcattgggtg aaatggcaaa tgaactgctg ctctccagta accgtgttta tccctcaaaa
     1321 cttatggaaa gcggctacaa gttcctccac cctgagctaa atcaggccct cattaacttg
     1381 aagtaagctg aagcttttct caatcagtat cttgatctca ctcaacattg ccggatcatt
     1441 ttgtaattcc tttttcttat gatggattga aatgaaataa ttttctcgtt cacttgctcc
     1501 agagtgggaa aagaaagatg ggtgctatcc cacatttttt cactttgaca aaaaatttat
     1561 ggcaaataaa accacagcca cgctgaacaa aggtattaca ataaataaac cggtcaacca
     1621 agcggctcct gctggttttg ctaagttaag tgtccatcac attttagtaa tctttttggg
     1681 aacccaagtt ctggtatcag cgtgactgaa ataaattaca ccccattttc cggaagtcga
     1741 gtttcccact ttttcccatt cggaattaat tattttttac tcagtcatga ttattcctat
     1801 tatttgatat ctgatgtaaa cagagctaaa acaaaataat cactgctcaa accacttccg
     1861 tcttcacttc tactttttta ggttttctac ttaccagtcc cttaaaatct tccaaaatca
     1921 tgtaaagcac cggaatcaaa acgaggatca caaaagttga aaacaacacg ccaaacgcta
     1981 aactgatggc cattggaatt aagaatcttg cttgtaagga ttgctcaaaa agcattggga
     2041 gcaaaccgaa aaaggtggta agtgtggtca ataaaattgg ccggaaacgg cgcactccgg
     2101 cttcaacaat tgcttgtcga attggagttc ctctctcgcg ggaacggttg atgaaatcca
     2161 ctaaaatcag tgaatcgttg accacaattc cggtcaatgc gacaattccc aaaacgctga
     2221 cgacactcaa actgtaaccc atcagcaaat gtcctagaag tgctccaacc attccaaacg
     2281 gaattgcagt catgatgata acaggttgga aatagctccg gaactgcaac gccaacaagc
     2341 tgtaaatcaa gagcaaggcc acaataccgc cttgttttac accgtccatt gtgttggttt
     2401 gcgcacggtg tgcgccttca aatgaatatt gcaattgcgg attttgactt ttaattttca
     2461 gcaaaagttc ttgtttgatg gagcgttgga tttctcctgt gttacccact ccggaatcca
     2521 ccaacgccct tacgctcaca attcgccgtc cgtcgattct ccggatggca ctgtaagatt
     2581 gaccgtattt taactcagcc acttgcctca aaggcaatag tttcccggtt ggtgacaaga
     2641 ttaccaaatc atccaaatcc ctcaaatatc ggcgttcctc gaacggtaag cgtagtttca
     2701 cgctcacttc atcgctatct cgcaacaatt taaaaacttc aacgccttga aaagcagctc
     2761 gaatttgctg agtcaattct tgtgtactta aacccaaagt tcgtccagcc ggactgagac
     2821 gcaattgcac ttcacgtttt ccgtcttcgg tgctgtcttc gatgttactg actccgggat
     2881 attcacccaa tttttcttta aattgctcga cgatgaccaa aagtttattt gtatcggggt
     2941 gactgagttg cagatcaata tcgtaatttg aacccatcga atgccgcgca cggatgttga
     3001 gtgcttcaat tccggcaact tcgcccattt ctttgcgcca tcttcttgaa aattcgactg
     3061 ccgcaaattt tcgttcattg agaggtttca gataaactcg taccgaagtt ttgttagcac
     3121 cgccccttcc gactgtactg taaattccat catgaacagg ttgtccggtt tcagcttcat
     3181 attcacggag caacttttct gcgctgcgca acattttttc ttcaacttca cgactgaccg
     3241 aggccggagt accaaaaggc attcgtgcgg tgactgcaat gttatcgcga tctatttttg
     3301 gaaaaaaagt aaatttcatg tgtccgcctg ccaccaatcc ggcgcaaagc aatataaaga
     3361 aaagaccgag cgcaaaaata gtgtaacggt aagcaatacc cttttccaaa attttccggt
     3421 atggtttgtt ggaaaaccaa aaaagcgcgg aactgaaata atgtcgcggc gtttccagaa
     3481 ttttcccaag cggcgcaagt atccaagcca tcacccgatt gatgtgtgag agatgtccgg
     3541 gaagaataaa catggcttcc agcaaagaaa agagcaaaac cacaatcaaa acatttggaa
     3601 tattttggaa aatatttccg gtgatgcccg gaataaaata gatcggaatg aaagctgcaa
     3661 tggtggtcag gattgtgatt aaaaccggcg ggccgatttc agttgcgccg tccacagcag
     3721 catccaatgg cgattttttc atttcctgat gcgcatagat attttcgccg accacaatgg
     3781 catcatccac cacaattccc aaaaccaaaa taaaggcgaa aagcgaaatc atgttgatgc
     3841 tggaatcggt gatcggcatc gaaatcatgg caccggcaaa actgatggga atgcccatca
     3901 taatccaaaa agacaaatcc actcttaaaa aaagtgacaa tgtcagaaag accaaaccca
     3961 gtcccaacca taaatttttc atcaataaat tgatgcgatc ttccaaaatg atggatgaat
     4021 ctctcacaat ttgtgttcgt acactagatg gcaattgact ttttatttct tccatttttt
     4081 catgaacggc ttttgaaaca tcggttggag tttgctcgcc gacacggtaa accaagattc
     4141 gttccgacgg ttttccgttg tatcgtgaac gaagatctga ctcctcaaat ccatctgaaa
     4201 tgtgggcaat gtcgcccaag cgcaaaattc cctggtcggt tgttagcagc ggaatttccg
     4261 catattccgc cgccgtgtaa cgacgctctt tggtgcgaat gagcagatca cctccgaccg
     4321 tttggatttt tcctccgggc atatcaagag ttgcctggcg gatgattccg gaaatttgat
     4381 tcagcgtgag agcatgttgc tgcaaggcag attttgaaat ttcgagtgtc atttcaaaac
     4441 ccctcggctg ttccacatcg acttgcgtga tgtccggatg cgtcaacaaa gtgtcccgag
     4501 ccatttgagc caactcaatc aaggaccgcg ccggtgcatc tccataaagt gcgatattca
     4561 aaacttctct ccgccttcga ggtaactgca cctgagcatc atcggcctct tcaggaagag
     4621 tcgtcaagcg agtaaccgcc gattccacat cgtcaaacac actttgtttg tccgctcctt
     4681 tttgcagttc aatccggaaa ctgcccatgc tttctttagc agttccggta atttttttga
     4741 ttccagttat tccggaaacc gcatcttcaa tcggcaacaa tattgattcc tcaatttcat
     4801 caggagttgc accagaatac ggaacagcta caataataaa atcgagatca aactcaggaa
     4861 acacttcctg tttgatatta gaaacaccca ttactccggc cactaaaata atgaccatca
     4921 gcaaatttgc cgcgacttta tgttctgcca tccaacgaat catgactcct ccttatgtga
     4981 ttcagaattt tgttttcttt ggttattctc tcgttgttgc cattttccat ccccttgttc
     5041 atcttttcca tctcctcgtt tccttcgcct tttcctttct cctgaaagct gcatccttct
     5101 tccgtccccc tttttcctga ttttttcttt cccggcaatg cggattttca gcccatttgc
     5161 agcacctttg atgggagaaa tgataatctg ttctcccggc tttacgccac cagaaagata
     5221 aatattgtct tgatcaagat gtgcgatttt cactcgccga acctccaact cgccctccaa
     5281 ggcaatccaa actgtgtcct gatcccgcaa tgcttgtgcc ggaatgttga ccacattttc
     5341 cagttgtctt ccgggaatgg tcacatcgac aaaagctccc acaaaaagag gcatattcga
     5401 ggtttgggga gtcggcggac tttccgaatt cgctgttccc acagtattaa ttagcgtgtt
     5461 ttcagaagtt cgtttgtttt tcgacttcac ctttgatttg cgtttcaggc gaaaggggtc
     5521 ctccacttca gctatcaatt gcaccatcat cccgcgcggt gtcagttcca gcaaatgccg
     5581 tttcaccgtc gcattccaaa cggacgcaga aatcattccg gctaaagaaa catttgcagg
     5641 aaattgaaat tgattttcgc cgtcaagtga tgtggaggat tgaggcagcc aatccatgcg
     5701 acccatcggc aaatcgatca cgacctcagc ctgatcactg cccaacaaaa ccgccaattg
     5761 cgttccggga ttaacatatt gccccaaatc cacatttttt gactgcacta ctccgttgaa
     5821 aggtgccttc aaacgcgatt gcgactgccg gatgccaatg ctcaaaagcg gatcgccttt
     5881 tttgaaaaaa ctgccttcgt tcatttttgg accaatccaa attacttctc caccaatacg
     5941 tgcgctcaag attacttttt ttgcagcacg aacgcgtcca tgagttctta cctcaatttg
     6001 tggattgctc aatgttgcct ccatcacctc caccaaagtt ccccgttgaa aatttggttt
     6061 tttgcgcgct ttcggcttat ttttcagcaa ggctacagta actcctgcac ctaatccaag
     6121 taccaaaaca gggagccaga attttaaatt aaataatttt ttccacatgt attcgtttca
     6181 gtcaaatagt tgaaggagat ttgggtaaat gcaaagaaaa cattgcccct tttcccgtat
     6241 tgctctgaac atcgattgta cctccgtgtg cttcgatgat tgttttgcac aaactcaagc
     6301 ccaacccgta gccgccggtt ttcctgttgc gtgacttgtc agtgcgataa aatggttcaa
     6361 aaatcaaatc caactcctca tcctgaatac cggggccgtg atcctgaatt tcaatgagcg
     6421 ttcctgcatc actctttttc aaacgaattt caaccggagc attttccgct ggagaatatt
     6481 tgagtgcatt ggccagtaga tttttcagta ctgtttcgat ttgttctgca tctgcgaaag
     6541 tttgacacgt ttccggggca ttctcaaagc gaatcccagg agtttgtcct tcaaaagtaa
     6601 tacaaatatc ctgaatcaac tttaccaaat ctgttttttg taaattcaac tgaccatatt
     6661 cactttttaa gcgggcagtt tccaaaattt cggtcaccat ttgctccatt tcctgaacat
     6721 catcgagcaa actttcacgc gaagcatctt ccggaataaa ttccaaagca actttcatcc
     6781 gtgtcaacgg cgagcgaagt tcatgactta catcgagtaa aagctgttct cgtgccgaca
     6841 acatttccct gattcgttct gacatacggt tgaaggcttt tgccaaattt cccagttcat
     6901 cacctcgttt ttccggaagt cgatgctcca aattaccttc gcctaaagca taaacaccat
     6961 ccgaaagcca gttaagaggt tgtaatattt tgcgtaaaat cagccaagtt cctgttaaaa
     7021 ccagcccaac caatcccaat actgcaaaaa tccacggcag tgcaaaacgg attccgtgcc
     7081 actcgtcctt aatttctgca aaaataaaac ggcctgtttt cgtttcaacg atcgccacac
     7141 cttgtctgtg atagcgccca aaacgcaaat cggttacaag tgggcgaagc ggttgtacta
     7201 gcaaacgaaa accgggatca ctagattttc ccatccactt gcggaaatgt attttctgca
     7261 aaggaggaat tcccgaccca gtttcccatg tttcattttc accttcatag cgaataatca
     7321 aaaacaaacg ctgcgataat tcacgtgcgt gttccaaatt aaggttttcc tctaaatccc
     7381 tgacaagata cgctccgtac tgggccaggt tattacggaa aaaatttctc tgattttccc
     7441 tgaaaccatg ccagctaatt gctgagatgc ccatcaccag taaacttcct gaaatcagca
     7501 taactgcaat caatttagta aaaacggagt taaaggggcg tttcagccat tcaagcctca
     7561 ttttcacctc caataaacaa atatcctgtg ccccagatag ttttgataaa acgaggattt
     7621 tttggatcat caccaagttt ttgacgcaag cggctcatgg taatgtcaac agagcggttg
     7681 aaggcatccc attcaatgcc tcgtaattct tcaataattt ggtcgcgatt gaggactttc
     7741 cctttgtttt ttaccagcaa aacaaggcac tcaaattcat ttaatgtcaa atgcaccaat
     7801 ttgtcatcaa cccggacttc gtatttatgg aaatcaatgc acaaagcacc aatagttttt
     7861 attccggatt tagattttgt ctgtatccgt cgtaaaatag cttgaattcg tgccaccaat
     7921 tcgcgcggtt caaatggttt tggcaaataa tcatccgccc caatttctaa accaacaacc
     7981 cgatccatca cttcgccgcg ggcagtaagc attaccaccg gaacgctact ggattggcgt
     8041 atggtacggc aaacttcaaa gccgtccatt tccggaagca tcacatccaa aataaccaaa
     8101 tccggagttt cttcttccag cttttccaat cctgcactcg gcaaggttgc tgtcagaaca
     8161 gtaaaaccca ttttgctaag ataatctgtc aaaagacgat tcagtttttc gtcatcgtcg
     8221 atgatcagaa tttttccgga aacggcagtg gttgaagtca tattgtttta agtttgacaa
     8281 gattcgcaaa gacgtatcgt gaatgaacaa atttttaaaa cctacttctt tgtagaaaaa
     8341 gtagggtgga tacgctatcg tttttctacc ataattttta tttgtatcgt ctctccaatt
     8401 ttcattgctc taaattcatc gtccggaagt ccggtttttt tcatagcatc tgccaataat
     8461 tccggaggct ctaaaaatgg ttcatgcgtt agttgaaaag ttccccaatg aatcggaatt
     8521 gatttttgcg aacgaatttc ttgatgaacc aaaactgctt gagccggatc aagatgctga
     8581 ttaagcatga aatatcttgg gccgtaagca ccaatcggaa tcattgccaa ttgaaacggg
     8641 cctaatttgt ctccgatttc cttgaatagt ttttcgtcgt atgctgtgtc tccggcaaac
     8701 catgatttaa aatttccaat ttgaactgcc caagatcccc ataaactttt gttggtgtcc
     8761 caaggtgtac gcttcgacca atgcacgctt ggagtaaaag tgattttcac agtaaggctc
     8821 cctttttttg aaaattggtg cggctcccac caatccaact caatcacttt ttcagtcgca
     8881 atcccgcgct ccaaaaacca cgctttcaag cccaaaggca ctaaccacgt cacggaatcc
     8941 ccaaacatat cgacggtacg ggaatccaaa tggtcataat gattgtgcga aataacaaca
     9001 aaatcaattt ccggcatttc tgcaaaagta agcgccggcg gcgtgaaacg ctttgcccaa
     9061 aaatcaaacg gagccgcata atcagtcaaa tgcgggtcgg tcaaaaaatt aattccttga
     9121 tattgcacta aaacagtagc atgtccaatc cacgttgcac gaggtacatc cgcagacgaa
     9181 gcggacctta agtcgatttg tgataactcc acctctgcta aaatagattc aatgtctttc
     9241 tgagcaacag aaggagtcgg cccttccttg aaacgcatcg acaaccactg aaaaatactt
     9301 gcgcttcttt tggttttaaa actatttttt ccatgatgca ctttattcga atcatattta
     9361 acattgtcgg aataatttgt tagcagcata gacacactca ccaaggtaac aatgctcaat
     9421 gcaatgaaaa tagcggtttt tttcatttat tattctttat tttttcagct ccggcaattt
     9481 tatccaaacg caaatgcaac tgcaattcca gttcatcttg tggatccgct ataagacctc
     9541 cggcaactaa cggcacatcc acatttaaag ctgaaaatgt ggtttttagt tgtccctcga
     9601 ccagaagaaa tccggcttgt tttgaaaacg acaacgcaaa cttcactgga aatattccgc
     9661 ctgcaacttc caaatttccg gaaacttccg gcagttgacc ttccatcatt ttatcccaag
     9721 ctgatttttc gagccattcg gaaacgaaac gaatattcgg agtgagatcg gctttgagaa
     9781 tttccggaat gtctccgtcg cgtaatgggt tgcctgaatc cagtgttttc acaggaacac
     9841 tgacttcaac ttgagccttt tctccggatt cttcccagtg catttctgct gtgacatcac
     9901 agcttttgcc aatcacatcc acatcggcaa aaaagaacat tgtttttact gttttgtagg
     9961 ccagacaatg ctcttccgga gcaaaaacgg gttcttcagg aaaagctaaa gttgaattca
    10021 gaagtagtaa aatgccagtt gtaataatta cgaaaaactt tttcatgctt gctgttcaat
    10081 gaatcgtgaa agaaatatac ttcgacatgt aatcagaagg cgggcatgca attccgccct
    10141 gccgtttgaa tgctcaatgc cagaaaccac ctcgatgtct accccagccg cctttttcag
    10201 cacgtttacg acgtttttcc attttctcca ctatttccgc ccgttgttcc ggagtcagtg
    10261 tggcatggaa ttttgcaaaa ctattggcga attttggcaa tttggctctt aactgttcaa
    10321 agcttccagt taatactgct tccaattttt ctgcatcagc ggtctcgctt ttcatttgag
    10381 ataatatttc atcgttaatc gtttgtctaa tttcctgcaa agattctttt tctgcaaaca
    10441 aatcttctgc aattggcgcg attttttgcg attgttcgtc gctgagcccc aatcgatctg
    10501 tcgcaagctc agtggcacgt cttgggctca tctctttatt tctgcagcct gcaagtgtca
    10561 atggaatcgt gacgacaacc aacaaaccca aaattatttt tctcaaacaa taacttgacc
    10621 tgtatttacg attattcata ataattcttt cagtactgaa gttttaaatg ctggtgaatt
    10681 caagtgattc ccattttaca gatagttttt tggaaaagct tttcccggca gtaacagggt
    10741 gtatcagttt gtatcagtat tagtgattga tggtttttct gaaaatagtt ttgaggaatg
    10801 acgaaaagta tttgttgtat cttgaatttt ggacccagat ttcatcatta atttaattgt
    10861 ataattgacc aaagcacttg agattgttaa actatggtat cttctttttt tagaacctgg
    10921 tattaaacca atttttttaa tgaagggaat ctatgagaat tgcaataatt gtctttgttg
    10981 gcattttatc ttattctgct gtagtactac cgggatattt ttattttgtt ccgggaggtg
    11041 ctgaggagat ttcaaattta aaaactttga cagaattgcc ggtggaagac gctgccaaat
    11101 cttcaacaga acttaacata gcaaatcctg gagacgctcc gcccctaaaa tgatccgtca
    11161 cttattatag ttctaattta aagaatttca ctcaattgcc gtaaacttat tttactacat
    11221 tattttttaa attatttgac aatcggaggg aaaatgccca ttcatctttc atttaaaaac
    11281 attcgaaact caaaagctgt ttccgagcac gtacatcagg cttttgaaga attgataaaa
    11341 attactgacg ataaatatcc ttttcacgtt aaactgaaca aagttgcaga taaatcttat
    11401 catgtgggca tcaattgcag ttacagaagc aagcaacttt ccagcaaagc cgatcatgaa
    11461 aatctttaca aagccctttc caaaggagtt gattccatga aatcccaggt aatccgtaaa
    11521 gccgaaaaag tccgcggctg aaaatttttc cacacggaga gctcgactgc cgtctcctgt
    11581 tttagattca aaagcttcat gtttggactt gtttctcacc atgtcaaata tcttcctgct
    11641 ggagtattgg cctttgcgtt tatattgtta ttctgcgtga atactcaact tcatgcagcc
    11701 tgtcgtggag aaggattcat gggtgtttcc actaatgatc caatcatgtc atgggttgat
    11761 ataacttcct ctccagttta ttcaagtgcg agcacatccg gaacactggg atgtaaaaat
    11821 tggaattttt cacaattctt agagcagtca cggatgcaat tccttcagca gtcccacaca
    11881 caattgctgg tggaaacagt acaaggacaa ggacctcatc tggaggcttt ggcaaagctg
    11941 atgacttgtc cgcaatcgtc tgcagaagcg ttttcaaata tgttatggga acatcgtcag
    12001 caaacagttc aaatttttga aacagcaaaa cagactccgg agttcttagc tgaactcagg
    12061 aaatggattg cggctaatcg gcaattgcgg aacaactgca gtctgtctta aatgaggaaa
    12121 aaataatgtg ctggaaaata gttctgcttt tggcaattgg aatatcactt tgcagtgaaa
    12181 ccctatgggc ctgtcacaaa ggcggaccca tgggatttgc tactaatgat ccgggaggtt
    12241 tttctctcga cattacactg tctccaactt ttaccggtgc aagtacacaa ggtacatctg
    12301 gttgtaaaga ctgggattac gccaagcatc aacggattca atatctggaa actcagtgga
    12361 catttttaag tgaagaggcc tctcaaggga aaggggagaa tctgatcgca ctggcacaaa
    12421 tgatgggttg tgccaaagaa agacaagcac agtttgcagt tttactccgg catcactatt
    12481 tttccctctt taaaaataca gatacccgca gtaaattttt actaaaactt gaaactcttt
    12541 taacgcaaaa tccgtcactc tcttgttctg gataaaacaa cttttaactc aaaaagctaa
    12601 aagtatgaaa aatgatttta tgatgcacct tgtaattgtt ggtggaatta ttttggtatt
    12661 ttctctgatt ttgaaaatca ttggtgtaac attttaatcc tcagttttag aaatagttcc
    12721 tctcaataat ttaaacctct ttttagttca agcacttgaa aagcggttaa tgtttttaag
    12781 cttcagtttt gaataatcat caaagaaagt ttgcattcag agataaaatg gcctaaccct
    12841 tgtattattt tgtctttgtt ataactttat aaccagaagt tgctgagatt tactctgtta
    12901 ctttaactga cacttttgcc aaaaaatgga gggttgaatg aattggctta aacgaactgt
    12961 aagcatttgt gcacttgctg gcagcatatt atttggcagc ataactagtg cacgtgcgga
    13021 aaccctgcaa attgtcttta acaatagtct ctggggtgct gcaatcggcg gtgtttctgg
    13081 gctcgctatc tgggctttgc aggatgaaga taaagaggat aaattatttc cacaatatgt
    13141 tgtaaaaggt atggccttag gcatgttcgc aggaatggga gtaggaattt ttgaggcccg
    13201 ttcagacggt ggcgttttca tgagtgatgg taatccaaag ggcctgctgc acttagatac
    13261 agatttacat ttgctaacct tgcgtccggc aaaacttttg cctctacctg aattcaatgc
    13321 agaaacagtt tcccctgaat ggcgtttaga tttgtttacc gcatctttct gaaccacaca
    13381 tgaacttgat gatcaccggt ggtgcgggct tcattggagc aaatttggtt ttgtactggc
    13441 tgcgtcatca tcctgatgat ttgctcgtca attttgatgc cctcacttat gcagggaatt
    13501 tgggcaacct ggaggacgta aaagggctgc caaactacca ctttttccac ggtgatttgc
    13561 gaaatgccct ggaaatttca aaggcttttt ctgaacataa tattgaagca gtaattcatt
    13621 tagccgcaga atctcatgtg gacagaagta ttcacgttcc tgctgacttt attgaaacca
    13681 atgtaacagg taccttcaac cttttagaaa cagcacgtga attttggggt gatgaactga
    13741 gtaaacatcg aattcttcat gtaagcactg atgaagtata cggttcgctt ggttccagcg
    13801 gaaagtttct ggaatccact ccatatgccc caaactctcc ctattcttct tcaaaagcag
    13861 caagtgacca ccttgtaaga gcctggcatc acacttatgg aatgaatgtg gtcacaacca
    13921 attgttcaaa taactacggt ccttatcaat ttcccgaaaa actgttgccg ctgctcataa
    13981 ataattgtac tcagaacctt cccatacctg tttatggaga tgggaaaaat attcgggact
    14041 ggttgtttgt tgaagaccac tgtgaggctt tggactgtgt ttttcatgag ggagataccg
    14101 gagaaacata caatataggc ggatgcaatg aatggagcaa tatcgatatt gtccgctatt
    14161 tgtgccgtga actggacaaa gagcttaatc gaagcggagc tgattcatgt gaaaatctaa
    14221 tcacttttgt taaggaccgt cccgggcacg accggcgcta tgcaatagat gcaggaaaaa
    14281 ttaaaaacac tttaaattgg actccgcgtc acacttttga ggaagggctg accaaaacca
    14341 tccgctggta tattgaccac caggactggg tcagtaacat tactaccgga acatatcgag
    14401 attactacaa acaccaatac ggttgattga attttgatca ccgttgatta ttaccaatta
    14461 acggttgatt gttgattgtg tcagctgaaa tctgtccaaa tcatgccatt gattcttgaa
    14521 gatgctttac tttctgcagc cgacgg
//
LOCUS       DTVD01000294            3895 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8573_length_3895_cov_297.760938, whole genome
            shotgun sequence.
ACCESSION   DTVD01000294 DTVD01000000
VERSION     DTVD01000294.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_3895_cov_297.760938"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="EYO68_10750"
     CDS             complement(<1..1277)
                     /locus_tag="EYO68_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate synthase/
                     dihydrofolate synthase"
                     /protein_id="HIC08320.1"
                     /translation="MSPPDKSYLQVLSELEQWGSYETSAQPRDISKLEGIRLLLKDLG
                     HPERAFKIIHIAGTNGKGLTATMISRLLCIQGFATGCYTSPHLIDIRERIALNDQYVS
                     KNIFVQSASLVLKIARSYKGTPYLSYFDVLTAIAFHVFMAENMEWVVLETGLGGRADS
                     TNVTDKEICVMTRIGLDHMEVLGRSLKEIAAEKTGIARTGIPVIIAPQATGLKPWLQK
                     HFNSENVPVYFVEDFFPEQFLEADITADFIPIPWVECFQTSLCTMQVLFNEDRLKKQI
                     WFEAVKKVKLAGRLDLRQNVTWSKHSLDFKTMLLDGAHNRDALMALSEYISKKNLIPF
                     TLILGMASDKLDDTLRSPLVELCSQAEKLIITPIPSPRTASPEMMGKFLDECGALAHS
                     PEIKYVNSAEEALEVSLDYREQPVVVAGSIYLVG"
     gene            complement(1413..2267)
                     /gene="hflC"
                     /locus_tag="EYO68_10755"
     CDS             complement(1413..2267)
                     /gene="hflC"
                     /locus_tag="EYO68_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIC08321.1"
                     /translation="MSGKLSKFGGIIILLAVGTIYLSTYIVDKTQYAIEILLGDPVDI
                     VMEPGLNFKLPFVSRIIFMENRLQDYDADPGAVFTKDKKEMKVDTYSKWRVTEPLKFY
                     ETVRTTSGAHARLDDIIYSQTREVLGQHTLVEIVSGNRKEIREAITLRARKNAENFGI
                     EILDVRIKRADLPEQNSQAVFGRMNAERRRQAKLYRSEGEEESLKIRSDADRERVEII
                     AEAKKINEETRGLADAKATKIYADAYQKDTDFFKFLRSHDVYRNSLQEGTTLLMDANS
                     KFFKYLKD"
     gene            2432..2695
                     /locus_tag="EYO68_10760"
     CDS             2432..2695
                     /locus_tag="EYO68_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08322.1"
                     /translation="MLKLRSPSQQEQILSLAMRQIEKMLKEMQTSGKDLSRLKLKKFQ
                     KTHHIDCKCKLCENAKTKNDLRLRGLLEDGFKVYVAGEKKNTQ"
     gene            2695..3597
                     /locus_tag="EYO68_10765"
     CDS             2695..3597
                     /locus_tag="EYO68_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIC08323.1"
                     /translation="MQNNLALNETYFSFCCTIPAELVELWNWFCFEKGALGTETLQES
                     SLESRLKIFYPKKPDGGAQKLFECFQREMNAKLEIRIHEEGSHPVENWQANWREHFHP
                     VEVGQSLIILPSWKTGIELSGRHPVWIDPGQGFGTGHHLSTALALEMLEQFLLETDTL
                     PESMLDLGTGSGILAIAACRLGIKKVTGLDIEAEAVAEVERNCELNGLSGRIIGHVGQ
                     PSFLKKPAPLVISNMLLSELLDIRLDLIRLTSPGGTLICSGLFGKQEEELRNSLTEMG
                     FVYRASLERENWNSIQFQRFGKES"
     gene            3599..>3895
                     /locus_tag="EYO68_10770"
     CDS             3599..>3895
                     /locus_tag="EYO68_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08324.1"
                     /translation="MIKYMRQSGLIVCCLFISLLVLSNSFAFGKKSSSKDLPWAQAEI
                     LILSQHPYWLRLLHYPSHSPMVGLWRSEIQNPSFFLDPDGNVNPAAELRKTLEAL"
BASE COUNT         1142 a          801 c          786 g         1166 t
ORIGIN      
        1 cccaccaaat aaatcgagcc tgcaacgaca accggttgtt cccgataatc taaagaaact
       61 tcaagtgcct cttctgcaga attgacatac tttatttcag gagaatgcgc caatgcaccg
      121 cactcatcta aaaattttcc catcatttcc ggacttgctg ttcttgggga cggaatcggc
      181 gtaataatca atttctcagc ttgcgagcac aattccacta gaggagaccg cagtgtatca
      241 tccaatttgt cagaggccat tcccaaaatc agtgtaaatg gtataagatt tttctttgag
      301 atgtattctg aaagtgccat cagggcatcg cgattatggg ctccgtccag cagcatggtt
      361 ttgaaatcga gagaatgttt gctccaagta acattctgcc gtaaatcgag acgaccagct
      421 aattttactt ttttgacggc ttcaaaccag atttgtttct ttagtctgtc ttcgttaaac
      481 aatacttgca ttgtacataa acttgtctga aaacattcca cccatggaat tggaataaaa
      541 tcggcagtaa tgtcagcttc tagaaactgc tcagggaaaa aatcttctac aaaataaaca
      601 ggaacgtttt cgctgttgaa gtgtttttgc agccaaggtt ttaatccagt cgcttgcggt
      661 gcaattatta ctggaattcc tgtgcgggcg attccagttt tttcagccgc aatttccttc
      721 aaacttctgc caagaacctc catatgatcc aaaccaattc tcgtcataac gcatatttct
      781 ttgtcagtca cattggtaga atctgctcgt ccacccaatc cggtttccag gacaacccac
      841 tccatgtttt ctgccatgaa tacatgaaaa gcaattgctg ttaaaacatc aaaatatgaa
      901 aggtatggtg ttcctttgta actgcgtgca attttcaaaa cgagggatgc actttgtaca
      961 aagatatttt ttgaaacata ttgatcattc agagcgatcc tctctctaat atcaatcaaa
     1021 tgtggagatg tgtagcaacc tgtggcaaat ccctgaatac ataataatcg gctgatcatt
     1081 gttgcagtca ggcctttgcc gttggttcct gcaatgtgga taatcttaaa tgctctttcc
     1141 ggatgcccca aatctttaag taaaagccgg atgccttcca atttactgat gtcacgtggt
     1201 tgagcacttg tttcgtaact tccccattgc tccagttcag aaagaacttg caaataactt
     1261 ttatcaggcg gagacattat gatttctcag gaggaatgca gccgaagttt ctttaagaaa
     1321 atcgactgta aagaacagaa gtccggtctt agccggaaat ggcgggaaaa attaattttt
     1381 caggtttagc taaaacagta ctgaaaaaaa acctaatctt taaggtactt gaaaaattta
     1441 ctatttgcat ccatcagcag tgtagtacct tcttgcagac tgtttcgata aacatcatgt
     1501 gagcgcagga atttaaagaa atcggtatct ttctgatatg catcagcata aatcttggtg
     1561 gccttagcat cagctaaacc acgagtttct tcgtttattt tctttgcttc cgcaatgatt
     1621 tcaacacgct ctctgtcagc atcagaacga attttcaagg attcctcttc accttcagag
     1681 cggtatagtt ttgcttgtcg tcttcgttct gcgttcattc ttccaaaaac tgcttgacta
     1741 ttttgttcag gcaggtcggc acgtttaatc cggacatcca gaatttcgat tccaaaattc
     1801 tcagcatttt tccgggcacg taaagtaatt gcttcacgaa tttctttacg atttcctgat
     1861 acaatttcca caagcgtatg ctgtccaagg acttcacggg tttgtgaata gataatatca
     1921 tccagccgag catgggctcc gctagttgta cgtacggttt cataaaattt aagaggttca
     1981 gtaacccgcc acttggaata ggtatcaacc ttcatttctt ttttatcttt ggtgaatacc
     2041 gcaccaggat ctgcatcata atcctgcagt cggttttcca tgaaaatgat tcgactgaca
     2101 aatggtaact tgaaattgag tcccggctcc atgacaatgt caactggatc acctaataaa
     2161 atttcaattg catattgagt cttatctaca atatatgtac tcaaataaat ggtgcctaca
     2221 gcaaggagga taataattcc cccaaatttt gacagctttc cagacatatt ttattaccta
     2281 gttagaaaat gaaaaagtag aagttgacac taatttttga caaaatgaga gtggcaacta
     2341 ttttcctgtt cataattgtc ggaaatgtcg taactcttgt caagttgaaa tagttaaacg
     2401 agccagaagc tccatctaat ttaaataaag tatgttaaaa ctgcgatctc caagtcaaca
     2461 ggaacaaatc ctctctctgg ccatgcgtca aatcgaaaaa atgttgaaag aaatgcagac
     2521 tagtggaaag gatttatccc gcttaaaact caagaaattt caaaaaactc accacatcga
     2581 ttgtaaatgt aaattatgtg aaaatgccaa aacaaaaaat gatctgcgtc tccgcggact
     2641 cctcgaggac ggtttcaagg tttatgtagc gggcgagaaa aaaaatactc agtgatgcaa
     2701 aataatttag ctcttaacga aacctatttc agcttttgtt gtaccattcc tgcagagttg
     2761 gtagaacttt ggaattggtt ttgttttgaa aaaggtgcgc ttggtactga aactttgcaa
     2821 gaatcttcac tggagtctag actcaagatt ttctacccaa aaaaaccaga cggtggtgca
     2881 caaaaattat ttgaatgttt ccaaagggaa atgaatgcaa agcttgaaat tagaattcac
     2941 gaagaaggta gccatccggt tgaaaattgg caggcaaact ggcgtgaaca ttttcatcct
     3001 gtagaggttg gacaatcact gatcattctt ccatcatgga aaaccggaat agaactttcc
     3061 ggaagacatc cggtgtggat tgacccaggt caaggttttg gaaccgggca tcatctttcg
     3121 acggctctgg cattggaaat gctggagcaa tttcttttgg aaacggatac tttgccggaa
     3181 agcatgcttg atcttggaac tggttccgga atccttgcta ttgcagcctg ccgtctgggg
     3241 atcaagaaag ttacaggttt ggatattgaa gctgaagcag tagcggaagt tgaacgtaac
     3301 tgcgaactga atggtctgtc aggaaggatc attggccacg tcggacaacc ttcctttctg
     3361 aaaaaaccag cacctcttgt aatcagtaat atgcttctgt ctgaattgtt ggatatacgt
     3421 ctggatctaa ttaggctgac aagtccgggt ggaactttga tttgcagcgg attatttgga
     3481 aaacaagaag aagaattaag aaactcttta acggaaatgg gttttgtcta tcgtgcttcg
     3541 ttggagcggg aaaactggaa ttcgattcag tttcaacgtt ttggtaaaga aagctgatgt
     3601 gataaaatat atgcggcaat ccggcctaat tgtctgttgt ttgtttattt ctctgttggt
     3661 cttgtcgaac tcatttgcat tcgggaaaaa atccagttcg aaagatctcc catgggcgca
     3721 agctgaaatt ctgatcctct cacagcatcc ttactggctg cgcctgcttc attatccgtc
     3781 ccactctcca atggttggtt tatggcgcag tgaaattcaa aacccctctt tctttcttga
     3841 tccggatggc aatgtaaatc cggctgcaga actaaggaag acactggaag cgctg
//
LOCUS       DTVD01000295            3861 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8713_length_3861_cov_389.957173, whole genome
            shotgun sequence.
ACCESSION   DTVD01000295 DTVD01000000
VERSION     DTVD01000295.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8713_length_3861_cov_389.957173"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2594)
                     /locus_tag="EYO68_10775"
     CDS             complement(<1..2594)
                     /locus_tag="EYO68_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIC08325.1"
                     /translation="MKKGLSRRNLFKYMGVGGATAVVAGCEKKPEKLIPMLVPPTDYE
                     YTPQTAYQYMTTCRECDAACGMMVTTREHRAQKAEGNPNHPVNQGALCARGQASLQTL
                     YNPNRHAHPLADGKQIPWEEGMQQFSAIIQAASGAIAYLGKPASGSEGDFVDEWLQAA
                     GGGQRFKFNLLTQKSQAEANKIAFDHADVPEYAFEKAGVVFNFSTDFLENWGNPVENA
                     RRFTDMHVYKNGRKNKFIHISPQVSLTGAKADRWIVINPGTEGLVALAIASVIQKENG
                     AYKFLKKYLQAYSPEKVAEATGVSAEILSELAIDAMEHGPLLALGGGNVSATDQSVET
                     LVAVNILNAVSGALNKTILFSDQTAPESSTHQDLTQLISDLNAGKIKLLIVDDSNPVY
                     ALPPSMGFLQAMKKAFVVSLASQKSETTHAANLVLPALTAYESWGDAFPRNGVRSIQQ
                     PVMSTVNMFDARAREDILLSVAQNVNKKAFSGNSSYLDYLQNIWQKIQRKVGDRRNFD
                     SFWISVLENGGIFEKPKFIRASLNRRVASVKLNDPGMAGSDGLTLLPTTSLLLGDGSG
                     ANKPWLQEVPDPMSQIVWDSWMEINPDTAQKLGINDRAIVEVSTPYGSVQATAYYHFG
                     IHRNAIAIPMGQGHQHSGDVADGFGINVMELLPDQMDKAGSLVFVGAKAKLKLINEKS
                     YTVNTDGNARQLGRDISAATTVEELNHPEKHSAGHNRPVEFYPDRSETAGYYKPYRWG
                     MTIDLDRCNGCSACVVACYAENNLPVVGKIRTAIGREMSWIRLERYIEGYGDDFETRF
                     SPMLCQHCGNAGCETVCPVYATYHNPEGLNAMVYNRCVGTRYCSNNCAYKVRRFNWFN
                     YEFPAPLD"
     gene            complement(2628..3359)
                     /locus_tag="EYO68_10780"
     CDS             complement(2628..3359)
                     /locus_tag="EYO68_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HIC08326.1"
                     /translation="MVLRYIFPIGLILSAFFLALSSSAALAEEQPIAFSHKLHTVQNG
                     IACQYCHLYARRSFSSGVPPVSTCIGCHGPQEQKLVQPESTEVNKMRTFWSNNEPIPW
                     VKIHDIPDFVRFPHKEHINADSNRLVDDAGAGCDSENVPRSLECKLAQFRTGGDERCL
                     ACHGDVRQMDVIVQVDENFGTMGWCMECHLQVKGAKERKRSGNTMAGWYHAKELDKKR
                     EADIGLVNEKGYHNPNLLDCYTCHY"
BASE COUNT         1058 a          880 c          786 g         1137 t
ORIGIN      
        1 tgatcaagtg gagcaggaaa ttcataattg aaccagttga atcgtcttac cttgtaggca
       61 cagttgtttg aacagtagcg ggttccaaca caacggttgt ataccatcgc gttcagacct
      121 tcaggattgt ggtaggtcgc ataaaccgga caaacagttt cgcaaccggc attcccgcaa
      181 tgctgacaaa gcattggcga aaaacgtgtt tcaaaatcat caccgtatcc ttcaatgtag
      241 cgctccaaac ggatccagga catttcacgt ccaattgcag tcctgatttt accgacaacg
      301 ggcaaattgt tttcagcgta acatgcaact acacaagcag aacatccatt acaccgatcc
      361 aaatcaattg tcatgcccca gcggtacggt ttgtaataac cggctgtttc cgagcggtcc
      421 ggataaaact caacgggacg attgtgtcct gcggaatgtt tttcagggtg gttcaactct
      481 tcaacggttg ttgccgcgga aatatcccga cctagctggc gggcatttcc gtcagtattt
      541 accgtgtagg atttttcatt gattaactta agtttggcct ttgcaccaac aaaaacaaga
      601 cttccagctt tgtccatttg atccggcagc agttccatca cgttaattcc aaaaccgtct
      661 gctacatctc cggaatgttg atgtccctgt cccataggga ttgcaattgc attcctgtga
      721 ataccaaaat ggtagtaagc tgtagcttgt actgaaccat atggtgtgct cacttcaacg
      781 attgcccggt cattaattcc cagtttctgg gcagtgtccg gatttatttc catccatgaa
      841 tcccagacaa tctgactcat aggatcgggt acttcctgca gccaaggttt gtttgccccg
      901 cttccatcac caagaagaag tgaggtcgta ggcaacaacg tcagaccatc actgccggcc
      961 atgccgggat cattgagttt gactgaggct actcttctgt tcaaagaagc tcggataaat
     1021 tttggttttt caaaaatgcc gccgttttca agtacactga tccaaaagct gtcgaaattt
     1081 cttctatctc caacctttct ttgaattttt tgccaaatat tttgaagata atcaagatag
     1141 ctggaattcc cggaaaatgc ttttttattg acgttctgag caacacttag caaaatatcc
     1201 tcgcgtgctc ttgcatcaaa catattcact gtcgacatta caggctgttg aatacttctt
     1261 actccatttc gaggaaatgc gtctccccaa ctttcataag cagtaagtgc aggcagaact
     1321 aaattagccg catgagtcgt ttcacttttc tgtgatgcaa ggctgaccac aaaagctttt
     1381 ttcatggcct gcaaaaaacc cattgaaggc ggaagagcat aaaccggatt agaatcatca
     1441 actatgagta atttgatttt tccagcattg aggtcactga tcaactgtgt caaatcttga
     1501 tgagttgaac tttcaggagc agtttggtcg gagaaaagga ttgtcttgtt cagcgcaccg
     1561 gagacagcat taagaatatt tacggcgacc agtgtttcca cactctggtc agttgcagaa
     1621 acattacctc cgccaagtgc taacaatgga ccatgctcca ttgcatctat tgccagttca
     1681 ctcagaattt cagcagaaac gcctgttgct tcagcaactt tttcaggaga ataagcttgc
     1741 agatattttt ttagaaattt gtaagcccca ttttctttct gtataactga agcaatcgcc
     1801 agagcgacca agccctcggt tccggggtta attacaatcc agcgatctgc ttttgcgccg
     1861 gtcagggaaa cttgaggaga gatgtgaatg aacttgtttt tcctgccgtt tttatagaca
     1921 tgcatatctg taaaacgccg ggcattttca acagggttac cccagttttc cagaaaatct
     1981 gtactaaaat taaatacaac tccagccttt tcaaaagcat attccggaac atctgcatga
     2041 tcaaaagcaa ttttgtttgc ttctgcctga cttttttgag tcagcaggtt gaatttaaaa
     2101 cgttgcccac ctcctgcggc ttgcagccat tcatcaacaa aatctccctc gctccctgat
     2161 gcaggttttc ctaaataagc gattgcacct gaagctgctt gaataatagc ggaaaattgc
     2221 tgcattcctt cttcccaggg aatctgcttt ccgtccgcca gagggtgggc gtgtcgattg
     2281 ggattataaa gagtttgcag tgaggcttgt cctcgcgcac aaagtgctcc ttgattaacg
     2341 gggtggttgg ggttgccttc agctttttgg gcacgatgtt cacgggttgt aaccatcatt
     2401 ccacaagcag catcacactc acggcaggtg gtcatatatt ggtaggcagt ttgaggagtg
     2461 tattcatagt ccgttggcgg gaccaacatc gggatcagct tctccggttt tttttcacaa
     2521 ccagccacta cagcggtagc accaccgaca cccatgtatt tgaataaatt tcttcggctg
     2581 aggcccttct tcattcggtt ctccttttca tttctcactt ttcgttttta ataatggcat
     2641 gtgtagcagt ccagcaaatt tgggttatga tatccttttt cattaaccaa tccaatgtca
     2701 gcctcacgtt ttttgtccag ctcttttgcg tgataccaac ctgccatcgt atttccagaa
     2761 cgttttcgtt cttttgcacc tttcacttgt aaatgacatt ccatgcacca gcccatagta
     2821 ccaaaatttt catcgacctg tacaattacg tccatttgtc gtacgtcacc atggcaggcc
     2881 aggcagcgct catcacctcc agttcgaaac tgagctaact tacattccaa acttcgcggt
     2941 acgttttcag agtcgcatcc tgcaccagca tcatctacca gacgattgct gtcggcattg
     3001 atatgttctt tgtgaggaaa tcgtacaaaa tctggtatgt catgaatctt aacccatgga
     3061 ataggctcat tattcgacca gaaagtccgc attttattga cctctgtact ttctggttga
     3121 accaattttt gttcttgtgg accatggcaa ccgatgcagg tactgacagg gggaactccg
     3181 gaagaaaacg agcgacgggc ataaagatga cagtattggc aggcaattcc gttttgaact
     3241 gtatgaagtt tgtggctgaa agcaatgggt tgctcttctg ctaaagccgc cgaggaactc
     3301 aatgcaagaa aaaaagcaga cagaatcagg ccaatcggaa aaatgtacct tagaaccata
     3361 ttattgtttc gcaagatctt aaaatttcaa gaatcaactc ataattaaat tcagttgtac
     3421 atgagatcat gaagattaag aaagttcaac ctatttttca ttgtttttga ttatttgtaa
     3481 aaacgagaac gttttctctt caagaatcaa gtctctgcta aaaaccggaa tcactcaatt
     3541 gtgcgatcgt ttctctactg gaaccatgga aggtacaaat aacaaaataa aaaccaatat
     3601 aaataatcgc ttacggattc agagatcaag attatttata ctcaaaatta tggctataca
     3661 tcaagcgaag tacgttttat tcagatgaac caaaattaag ctgtagagtt aagttaaagg
     3721 aaaggacgtt taagtcggta ctaacctcac tccgtcggtc ttcgtcacca atctgagcag
     3781 tagcagaaag tgctggattt actgcataat ttttatcttt aattcttgtt caattccggt
     3841 aatagaagcc tggtaaatgc t
//
LOCUS       DTVD01000296            3824 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8861_length_3824_cov_278.163173, whole genome
            shotgun sequence.
ACCESSION   DTVD01000296 DTVD01000000
VERSION     DTVD01000296.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8861_length_3824_cov_278.163173"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..3110)
                     /locus_tag="EYO68_10785"
     CDS             complement(<1..3110)
                     /locus_tag="EYO68_10785"
                     /inference="COORDINATES: protein motif:HMM:PF00171.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIC08327.1"
                     /translation="MNSFTQKKTDSPEIIRKTLALAESWQNRANTLLTKNEKKRQQMM
                     SRLFNHPSDKTVVMHLVDQSFRSGNPHRVVDQFRYLLEHHGIPRFFPLVEQCLLKIFL
                     RIGNLVPQISHSQILRKIREDTSRTILPEEAEFLKAHLNKHQTEGVQVNINHLGEAVQ
                     GDREALNRLRHYEDSLRNPDIHTISIKISNIVAQLHPLGFENELALICERLSELYRIA
                     LENPVLHSDGRRHAKFVNLDMEAYHDLDLTMSAFMETLDQPELKDLEAGIVLQAYLPD
                     SFLRQRELVKWAKKRVQNGGSPVKMRLVKGANLVMEAMEAEVSGWPLTVFDSKIETDA
                     NFKRMLEYGLLPENIEAVHLGLASHNLFDLAYTKILAEERDISVGLVFEMLEGMADHV
                     RRTIQKENQSGNNKKIDLLIYTPVTSQEQFISAIAYLVRRLDENTSPDNFLRHSFDIR
                     VGSTEWKFLEKQFLDSWKLKDEVSSAPRRQQNRVKEVFPDNIGTFGDPIFRNEADTDW
                     VLQENRKWAEKIRKKWQPTEKSKHKKIPLIIGKSEIKSRRKQQLFFDPSQPKTPLAPD
                     VPAVYSVALATKTDVNKAVKIAKEDPEQWRETTLLKRHELLSAAARNLRKRRGDLIGI
                     AAAVCGKTFYESDPEISEAIDFVEYYPHSLRLLEQQTSLGLSPLGVVLVISPWNFPIA
                     IPTGGVAAALACGNSVLFKPSPLAFPLGEEIAQCFWDAGIPREALQLVCCEDGEPIQT
                     LSGHPDVDTIIFTGGTHTALKMLEKRPDAELSAETGGKNATIVTALADRDQAVEHVLH
                     SAFSHSGQKCSATSLLVLEREVYDDETFRRQLLDAIQTLKVGSVWNFSNKMGPLIREP
                     MADFRKGLETLEDDESWALPPKCDETNPKLWHPAVKWGVRRGSFSHQTEFFGPLLSVM
                     KAESLEDAIEIVNGTDYGLTSGLESLDRREQKIWSEKIEAGNLYINRVTTGAIVLRQS
                     FGGMGLSSIGAGIKAGSPNYAVQFCKIEESDSPTQGPLREESPLLYIARNWQTQLSRN
                     EHAEIW"
     gene            complement(3375..>3824)
                     /locus_tag="EYO68_10790"
     CDS             complement(3375..>3824)
                     /locus_tag="EYO68_10790"
                     /inference="COORDINATES: protein motif:HMM:PF02632.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIC08328.1"
                     /translation="FPVPMTLQTFAVFLIGLTYGWRLGGVTVALYLLEGALGLPVFSG
                     GKGGLIVFMGPTAGYLVGFLVAATACGWFAERGFDRSYSKLFASLLVGNILLYAPGLL
                     WLGSLIGWDKPVLEYGLYPFILGDLLKIAMAVLLLPTAWKIVNRLKQ"
BASE COUNT         1049 a          916 c          811 g         1048 t
ORIGIN      
        1 ctccagattt cagcatgttc atttcgcgat agctgagtct gccagtttcg agcaatataa
       61 agcaaagggg attcttcccg caacggtccc tgcgtcggtg aatcgctttc ctcaatttta
      121 cagaactgca ctgcgtaatt cggacttccg gccttgatgc ccgcgccgat ggaagaaagc
      181 cccattcctc caaatgactg ccgtaagacg atcgcaccgg tagttacacg gttaatgtaa
      241 agatttcctg cttcaatttt ttccgaccag attttttgtt cccggcggtc aaggctttcg
      301 agtccggaag tcaagccata atcggttcca ttaacgattt caatagcatc ttccaaagac
      361 tccgctttca tcacagaaag taggggacca aaaaattctg tttgatgtga aaaactgccg
      421 cgcctcacac cccattttac tgccggatgc caaagttttg gatttgtttc atcgcatttt
      481 gggggcaaag cccaactctc atcgtcctcc aaagtttcca agcccttacg gaaatcggcc
      541 atcggctccc gaataagcgg tcccatttta tttgaaaaat tccagaccga acctactttc
      601 aaagtttgaa tagcatccag cagttgcctg cggaaagttt cgtcatcata aacttcacgc
      661 tccaaaacaa gtagagatgt cgctgagcat ttttgtccac tgtgactgaa cgcggaatgt
      721 aaaacatgtt cgactgcctg atcccggtcg gccaaggcag ttacgatggt tgcgttttta
      781 ccacctgttt ccgccgacaa ttctgcatcg ggacgtttct ccagcatctt caaagccgta
      841 tgtgttcctc cagtaaaaat aatagtgtcc acatccggat gtcctgaaag cgtttgaatc
      901 ggctctccat cttcacagca gacaagttgc agagcttcac gcggaattcc ggcatcccaa
      961 aaacattgtg caatctcttc ccccaaagga aatgccagtg gtgatggttt gaacaaaaca
     1021 gaatttccgc aagccaacgc agcagcgact ccaccagtcg gaatggcaat tggaaagttc
     1081 catggagata tcaccagaac cacgcccagg ggagataacc cgagcgaagt ttgctgttcc
     1141 agcaggcgca gtgaatgtgg ataatattcg acaaaatcaa tggcttctga aatttcagga
     1201 tcgctttcat aaaatgtttt tccgcaaact gcggcggcaa ttccaatcaa atcaccacgc
     1261 ctttttcgca aattacgtgc ggctgcagaa agcaactcgt gccgtttcag caatgttgtt
     1321 tcacgccatt gttccggatc ttctttggca attttgaccg ctttgtttac atccgttttt
     1381 gtagccagcg caacggaata tacagccgga acgtcaggtg cgagaggcgt tttcggttgt
     1441 gagggatcga aaaataattg ttgtttacgc ctggatttga tttcagattt gccgataatg
     1501 agtggaattt ttttgtgttt gcttttctca gtgggctgcc attttttacg tattttttcg
     1561 gcccattttc gattttcctg aagcacccag tctgtgtctg cttcgttccg gaagatagga
     1621 tccccgaagg ttccaatgtt atccggaaaa acttccttta cgcgattttg ctgacgccgc
     1681 ggtgctgaag acacttcatc tttcaacttc catgaatcga gaaattgctt ttccagaaat
     1741 ttccattcag ttgaaccaac ccggatatca aatgagtgcc gtaaaaaatt atccggactg
     1801 gtgttttcgt caagccgcct taccaaataa gcgatggcac taatgaattg ctcttggctt
     1861 gtcactggag tgtaaattaa caaatcaatt tttttgttat taccagactg attttctttc
     1921 tgaatggttc tgcgtacatg atcagccata ccttcaagca tctcaaaaac caggccaaca
     1981 gaaatatcgc gttcttctgc aagaattttt gtgtaagcca ggtcaaataa attgtgtgaa
     2041 gccaaaccta aatgcacggc ctcgatattt tccggtagaa gtccatattc gagcatccgt
     2101 ttgaaatttg catccgtttc gattttggaa tcaaacacag tcagcggcca gccagaaacc
     2161 tctgcctcca ttgcttccat caccagatta gcgcctttta ccaatcgcat tttaaccgga
     2221 ctgccgccgt tttgtacccg ttttttggcc cacttgacaa gttcacgctg acgcaaaaac
     2281 gaatccggaa gataagcctg caaaacaatt ccagcttcta aatcctttaa ttccggctga
     2341 tcgagggtct ccatgaatgc actcatcgtc aaatccagat cgtgataagc ttccatatcc
     2401 aaattgacga atttggcatg tcgcctaccg tcactgtgca aaaccggatt ttccaaagca
     2461 atccggtaaa gttctgaaag ccgttcgcaa atcagtgcaa gttcattttc aaaacctagt
     2521 ggatgaagtt gtgcgactat attggatatt ttgatggaaa tggtgtgaat gtccggattg
     2581 cgaagagaat cttcgtaatg ccgcaaacgg tttagagctt cacggtcacc ctgcaccgct
     2641 tcacccaagt gattgatgtt gacctgaacg ccttcggttt ggtgtttgtt taaatgtgct
     2701 ttaagaaatt ctgcttcttc gggaagaatt gtgcggctgg tatcttcacg aattttgcgt
     2761 aaaatttggg aatgcgaaat ttgcggaaca aggtttccta tccgcaaaaa tatcttgagt
     2821 aaacactgct cgactagggg gaaaaagcgg ggaatgccat gatgttcaag taggtaacgg
     2881 aattgatcaa ccactcgatg tggattcccg gaacgaaaac tttgatcgac caaatgcatc
     2941 acgacggttt tgtcggacgg atgattgaac aaacgactca tcatctgctg tcgcttcttt
     3001 tcatttttgg tgagtaaagt gttggcccga ttttgccagc tttcggcgag agcaagggtt
     3061 tttcgaatga tttccggaga gtcagttttt ttctgtgtga aagaattcat cctctgtcat
     3121 tattcagcca agttaaatca atctctctca aaacaaagta tggaagaaat gatcttcagt
     3181 gccgttactc aggaattcac gaccagccca gacatacatc tggattcagg actaaagcaa
     3241 acttggaagt ttttaatcaa cgttccctag tttctttttg aagggaactg tctcaagaag
     3301 tttgaaggga ggaaccatcc atgaatcgcc gtataaatta atgagcgacg aggaaccgga
     3361 atgtaccaaa aaaattactg tttcagccga ttaacaattt tccaagcagt cggaagcaga
     3421 agcacagcca ttgcgatttt taacaagtcg cccagaatga aaggataaag cccatattct
     3481 aaaactggtt tatcccaacc gatcaggctg cccaaccata acaggccggg agcataaagc
     3541 agaatattgc ccaccaatag ggacgcgaat aatttggagt aactgcggtc aaatccgcgt
     3601 tccgcaaacc acccacaggc tgtggccgcc actaaaaatc caactagata acctgctgtt
     3661 ggtcccatga agacaatcag tccccctttt ccaccggaaa ataccggcaa tccaagtgct
     3721 ccctccagta aataaagagc aacagtgact ccacctaaac gccagccgta tgtcaaaccg
     3781 atcaaaaaca ccgcaaacgt ttgcagagtc attggaaccg gaaa
//
LOCUS       DTVD01000297            3805 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_8925_length_3805_cov_266.820000, whole genome
            shotgun sequence.
ACCESSION   DTVD01000297 DTVD01000000
VERSION     DTVD01000297.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8925_length_3805_cov_266.820000"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            507..2267
                     /gene="frdA"
                     /locus_tag="EYO68_10795"
     CDS             507..2267
                     /gene="frdA"
                     /locus_tag="EYO68_10795"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HIC08329.1"
                     /translation="MALQYDIVIVGGGGAGLRAAIAIGETNPKLKVALVSKVYPMRSH
                     TVAAEGGAAAVIKDNDSFNDHCLDTIGGGDWMCDQDAVELFVNEAPKELIQMEHWGCP
                     WSRELDGTVAVRPFGGMKIERTWFAADKTGFHLLHTLFQTSLKYNNVIRHDEWFATKI
                     LVENGRCQGVTAIEMRSGILKVIAAKSVIMCTGGAGRVFPFTTNGSIKTGDGMAMAYR
                     AGIPLKDMEFIQYHPTGLPGTGILITEAARGEGGILVNKEGYRYLQDYDLGMPLDIED
                     PKHPVKKSMELGPRDRLSQAFIKEREKEKTIPGPFGDVVHLDIRHLGKKKIDKKLPFV
                     RELTKNYVGIDPVYEPIPVRPVVHYMMGGIDTNIDCSTILPGLFAAGECACVSINGAN
                     RLGSNSLTEILVYGARAGQAAAVFAAEHSEINLEALEKQGADEYKRITRDFLQKDGGS
                     ERIATLRKEMHQVMEQSVDIFRSESGLKEACNKIEELKDRFSSILIEDKSLNFNTELT
                     SALELENMIDVAESIIFSALSRRESRGSHQRTDFVNRDDENFLRHSLAYRTEAESRIE
                     YQDVVITKWPPAERIYGKEE"
     gene            2264..3022
                     /locus_tag="EYO68_10800"
     CDS             2264..3022
                     /locus_tag="EYO68_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIC08330.1"
                     /translation="MSVSESFELEIFRYCPEQDTEPFFKSYEVPFREDWVVLDAINYI
                     KDEIDGTLSYRWSCRMGVCGSCGMMVNGEPKLTCSAFLKDYPSRKIRVEPLQGFPVER
                     DLIFEMDDFMNKLTEVKPYLLRKEGEEKPLEEGEYLQSPAELAHYKQYTLCINCMLCY
                     AACPIYALDSSFVGPAAIALAQRYNLDSRDQGRNQRQEVIASHEGIWECTFVGECSVV
                     CPKDVDPAAAIQRAKVSSTLDWFKEMMIPRSKGK"
     gene            3019..3402
                     /locus_tag="EYO68_10805"
     CDS             3019..3402
                     /locus_tag="EYO68_10805"
                     /inference="COORDINATES: protein motif:HMM:PF02300.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08331.1"
                     /translation="MTTYRKPVSNTWWLESKPYFFFVIREFTSLFVAGYCLFLLVFIA
                     KAGDSQEAYTTLIFSLKSPISMVLHLISFTFILYHAITWFNLTPKIMVLHLGEERIPN
                     ALIAGAVYLSWGVVSLIISWLILGG"
     gene            3404..3748
                     /locus_tag="EYO68_10810"
     CDS             3404..3748
                     /locus_tag="EYO68_10810"
                     /inference="COORDINATES: protein motif:HMM:PF02313.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HIC08332.1"
                     /translation="MSKSNEPVLWLLFSAGGMISAMVFPMLLIITGVILPFELAGEVT
                     FERIHNAVKNPVVKLVLFFVIALPFFHWAHRFRFTLIDIGLKPISFLISLLCYGGAIT
                     GTIVAAFILWRI"
BASE COUNT         1035 a          764 c          906 g         1100 t
ORIGIN      
        1 tttctgatcc tcagggctcc agccggattt cggctcacct gattctatta ccgaaaggcc
       61 tactaccggt agctctttct gacaccatcg gcctgcgcgg tgggaggaag acctcagaat
      121 taccgtggtg aacggttttt gcttctgttc cagaatttgc accgctattt cctttacctc
      181 gctcttgtct tttgcgttat tctcagctac gatgtcattc tctcgttctc ttttgaagat
      241 ggcctcggcg taggagttgg aacagtggtt tagtttgagt ggtatttaaa ttatttttca
      301 ggtttccagt tcaactattt gcaataaaga atttcgttca gagggttctt gcgatatgca
      361 gatttaatct cacaattacg caatttagaa caaaattaat ttagtacttt cccttaattg
      421 ggcctatatt ttttaatttt gcttttataa aataaataat taaaggtgaa tttggttatt
      481 aaaatgacac tattaggtta atacaaatgg ctcttcaata tgatattgtt atagtgggag
      541 gaggaggtgc aggattaaga gcggccattg ctattggtga aacaaatccc aaactaaaag
      601 tcgctttggt tagtaaggtt tatccaatgc gaagtcatac tgtggctgct gaagggggtg
      661 ctgcagcggt tatcaaagac aatgatagtt tcaatgatca ctgtttggac accattggag
      721 gaggtgactg gatgtgtgat caggatgcag tggaactatt tgtgaatgaa gctcctaaag
      781 aattgataca gatggagcac tggggctgtc catggtctcg ggagcttgac ggtactgtag
      841 ctgtccgccc gttcggagga atgaagatag aacggacttg gtttgctgct gataaaacgg
      901 gcttccattt gctccataca cttttccaaa cttctctgaa atacaataat gtcatccgtc
      961 atgatgaatg gtttgctacc aaaattttgg ttgaaaatgg gaggtgccaa ggtgtaacgg
     1021 ccattgaaat gcgttccggg atcctgaagg tgatagctgc aaagtcggtt atcatgtgca
     1081 cagggggagc aggccgtgtt tttcccttca ctactaacgg gtcgattaag acaggtgacg
     1141 gaatggctat ggcctaccgg gctggtattc cattgaagga catggaattc atacagtatc
     1201 atcccacagg tctgccagga acaggtatac ttattactga agcggcccgg ggagaaggag
     1261 gcatccttgt taataaggaa ggctaccggt atctccaaga ctatgatctg ggaatgcctc
     1321 tagacattga agatcccaag catccagtca aaaaaagcat ggagctaggc cccagggaca
     1381 ggttaagcca agcctttata aaagaacgag agaaagaaaa gactatcccc gggccttttg
     1441 gagatgtagt acacttagat atacgacatt taggaaagaa gaaaattgac aagaaacttc
     1501 catttgtaag ggaactcaca aaaaattacg tagggattga tccagtctat gagccaattc
     1561 ccgttcgacc tgtagtccac tacatgatgg ggggtatcga tacaaatata gattgcagta
     1621 caattttacc tggacttttt gctgccggtg aatgcgcctg cgtttccatt aatggggcca
     1681 atcgactagg atcaaattca ctgactgaaa tcttggtcta tggtgcccgc gcaggacagg
     1741 cagcagcagt ttttgccgcc gaacattccg agattaatct ggaagcactt gaaaaacagg
     1801 gagccgatga gtataaaaga atcactcgcg acttcttgca aaaagatggc ggttctgagc
     1861 gaatcgccac cctccgtaag gagatgcatc aagtcatgga acagagtgtg gacatctttc
     1921 gttcagagtc aggtttaaag gaggcatgca acaaaatcga agaattgaaa gaccgatttt
     1981 cttcgatact tattgaggac aaaagtctga atttcaatac agaattgact tcggctttag
     2041 agcttgagaa catgattgac gttgcagaat caatcatttt tagcgcattg tcaagaaggg
     2101 aaagtcgagg ttctcatcaa cgcactgact ttgtaaacag agatgatgag aattttctgc
     2161 ggcattctct tgcgtatcga accgaggctg aaagccgtat cgaatatcaa gatgtcgtta
     2221 ttactaaatg gccaccagcg gaacgtatct atggaaaaga ggagtgagcg tgtctgaatc
     2281 ttttgagttg gaaatttttc ggtattgtcc tgagcaggac actgaacctt tttttaaaag
     2341 ttacgaagtc ccctttagag aggactgggt ggttctcgat gccatcaact acataaagga
     2401 tgaaattgat ggcacacttt cctatcgctg gtcctgccgc atgggtgtct gtgggagttg
     2461 tggaatgatg gtcaacggtg agccaaaact gacatgttca gcttttctga aagactaccc
     2521 ctcccgtaaa attcgtgttg aacctctgca aggattccca gtagagcggg atctcatctt
     2581 tgagatggac gatttcatga acaaactgac agaggttaaa ccttatcttc tcagaaagga
     2641 gggggaggaa aaacctcttg aagaaggtga atatcttcag tctcctgcag agctagctca
     2701 ctacaaacag tacaccttgt gtattaattg tatgctctgc tacgcagcct gtcccatcta
     2761 tgcacttgac tccagctttg ttgggcccgc ggctatcgca ttggctcagc gctataactt
     2821 ggattcgcgg gatcagggcc gtaatcagcg tcaggaggtg attgctagcc atgaggggat
     2881 ctgggaatgc acttttgtag gagagtgcag cgttgtatgt ccgaaagatg tggacccagc
     2941 agcagcaatc cagcgagcaa aggtttccag taccctggac tggttcaagg aaatgatgat
     3001 tccacggagt aaggggaaat gaccacctac cgaaaacctg tttccaacac ttggtggttg
     3061 gaaagcaaac cctatttttt ctttgtgatc cgagaattca ccagtctttt tgtcgcaggc
     3121 tattgcctgt ttctgttagt gtttattgca aaagccggag acagtcagga agcctatacc
     3181 acactgattt tttctttaaa gtctcccatc agcatggtgc ttcatctgat ctcattcacc
     3241 tttattttgt accatgccat cacatggttt aacttgacgc caaagatcat ggtactccat
     3301 cttggagagg aaagaatccc gaatgcgtta atcgccgggg cagtttatct cagttggggg
     3361 gttgtctccc tgataatctc ttggctcata ttaggaggtt aaaatgtcaa aatcaaatga
     3421 acctgtcttg tggttgttgt tttctgctgg tggcatgatc tctgctatgg tgtttccaat
     3481 gcttctaatc atcactggag tgattctacc gtttgaatta gcaggggagg tgacttttga
     3541 aagaattcac aatgcagtaa aaaatcccgt cgtcaaactg gtcctttttt ttgtgattgc
     3601 tctgccattt tttcattggg cacaccgttt tcgctttaca ctcattgata ttggtctgaa
     3661 gccaataagt ttcctgatct ctctcctctg ttatggtggt gccattaccg gaaccatagt
     3721 cgcagcattt attctttgga gaatataaaa ttataattaa acaccacgca tcattaagta
     3781 tgaaaagtct aaatttgact aatca
//
LOCUS       DTVD01000298           14242 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_894_length_14242_cov_212.844294, whole genome
            shotgun sequence.
ACCESSION   DTVD01000298 DTVD01000000
VERSION     DTVD01000298.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_14242_cov_212.844294"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="EYO68_10815"
     CDS             <1..556
                     /locus_tag="EYO68_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIC08333.1"
                     /translation="VKKRNDQDWTYLPYGPFDRFENYLKWMKNACSGKDPLFHVIKDA
                     ITQKALGVASYLRIEPILGVVEVGHIHFSPVLQKTPHATEAMFLMMIRVFDGLGYRRY
                     EWKCDALNSRSIKAAERLGFKFEGIFRQDKIYKGRNRDTAWFSIIDKDWPNLKNAFQS
                     WLNPENFDTDGQQILSLTEIRNNQ"
     gene            562..1002
                     /locus_tag="EYO68_10820"
     CDS             562..1002
                     /locus_tag="EYO68_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HIC08334.1"
                     /translation="MKVTVYLSGEIHTDWRNEIKDGAQKYGLDIEFVSAVTDHDASDS
                     AGDVLGPEDSSFWRDHKSAKVNNIRTKTLIKKSDLAVIRFGEKYKQWNAAFDAGYCAA
                     SGTPYVTLHADDIVHPLKEVDASAMAWTKTTEQVIEILKYLTKA"
     gene            1030..1689
                     /locus_tag="EYO68_10825"
     CDS             1030..1689
                     /locus_tag="EYO68_10825"
                     /inference="COORDINATES: protein motif:HMM:PF01571.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08335.1"
                     /translation="MIKTSPLSGIYRNHPVKLTEGSGWQIAENFGNDERESLHLSHDS
                     ILVDWSHIGKISLSGGDAAKAAELTFKGASKLEPLKTLSNKDIAVLRLTGNDYLILCQ
                     PGMETELLGKIDPKITTVIDQTGSQGCLVLGGPRRDEVLQRSTAVDLRRDKVVAGSVL
                     QSSIHTVGMTLYRTKNFDIMLHPRNLSESLYDALMDVGIGIGLVPGGINTVPVSFEEG
                     K"
     gene            1689..2888
                     /locus_tag="EYO68_10830"
     CDS             1689..2888
                     /locus_tag="EYO68_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC08336.1"
                     /translation="MTQRMWQNHDLKKNYDVVIIGGGAHGLATAFYLAKNHGITDVAV
                     LEQKFIGYGGSGRNTAILRSNYRTEEGIKFYDQSLDLYEDLAGELDFNLMFSQQGHFT
                     LGHSTASISGLVTRAENNKALGIDSFMLDPKEIKKMLPEIDITDHPRFPVHGALYHPP
                     GGIIRHDAVVWAYARGADRKGVQIHQMTEVQDILVENGKATGVVTNRGTINCNTVISV
                     VAGWSSEVCKKVNVKLPFVTHPLQALVTQSYKPWLHHVVVSGTLHIYLSQTDRGELVC
                     GNGIDTYPHYGMRSTLGFLESYAAHVLELFPVLHNVAVQRQWAGLCDMTPDFSPIISP
                     IDEIDGFYVNGGWGTYGFKASPASGYNTAQMVANGKTPEMIEPFRYNRFAENRLVGEK
                     AAASVGS"
     gene            2992..3777
                     /locus_tag="EYO68_10835"
     CDS             2992..3777
                     /locus_tag="EYO68_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HIC08337.1"
                     /translation="MEIIVPIKQVPDVALNIKVKDGAVVEEGLSYVISSWDETALEAA
                     LQMTEDVGGEVTLLTVGPEKASESLRKGLAMGAHKAIHVKYDFAKETDSFAYAKILQK
                     VLEGRNYDLILTGKQAQDTDAGLTAPMLAEFLGLPQVTNIIRFSDVSDQNITLYRQGD
                     HATEVLEMNLPGVVTVNDSLNEPRLASMRGIMMAKKKPLEEVDLDALGVSAEMRGAQA
                     SMTEVLQYDKPESRKEGKIFEGDEADSVKQAMELLVSEAKFLA"
     gene            3869..4831
                     /locus_tag="EYO68_10840"
     CDS             3869..4831
                     /locus_tag="EYO68_10840"
                     /inference="COORDINATES: protein motif:HMM:PF01012.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIC08338.1"
                     /translation="MSKILIVAEVKNGEIKKNTLELLSFAKSQGLENEAVLIGSGVSG
                     QADILAGQGASTVYLGDDPSLEIYNTEQYTALVSDAILQSGATQVWLSSSELGRDLTP
                     RVAARQSVGALSDVTQLEISGDEITAYRPCMSTKVIQKCKFSKDGLRVISIRSGFFTA
                     DETSPATAKTVSLSIPEGHSKLKVKEVQVEVSDEVELNEARIVVSAGRGVGGTEGCDF
                     VKPLATDLGAAFGASRAVCDAGWLPHKHQVGQTGTMVTPDFYFALGISGAIQHLAGMS
                     GSKVIIAVNKDPDAPIFKVADYGIVGDLFKAVPVLREEVQKLKA"
     gene            4870..7308
                     /locus_tag="EYO68_10845"
     CDS             4870..7308
                     /locus_tag="EYO68_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC08339.1"
                     /translation="MKSHYRAVVIGGGVVGASALYHLAKMGWNDVVLVEKIEYTSGST
                     WHAAGLLPLFNMSYSVGKIHKYSVDLYQSLEEETGQHVSFHKTGNLRLARNQERMDEY
                     KRYCGTASTIGVPFEMIGPAEIKKLWPFAKVDDLVGALFHPDDGHVAPVDVTMALLKG
                     ARANGAETYSEVEVTAMAQKPNDEWVVSTNKGDITCDVVISCTGNYARETGRMVGLEL
                     PVVPVEHQFIVTGPIPELVEYNRAGNPEMAVLRESDASYYMRQEADGLILGPYEKGAP
                     CWALDGVPEGFEKELLPPDLERLEPHIIAAGERVPIFETAGIKDHINGPIAYTPDGNP
                     MVGPAWGLRNFWLSEGHSFGITAAGGSGRHLAEWIVEGSPSIDMNGVDPRRFSATQST
                     RDFIKAKNEECYEHVFVIHYDFEERTAARPAKTSQIYDRQKTLNAAFGQRYGWERPNW
                     FAPQGSEPFNEYSFHTRRTNWFETVGEEVRAVREQVGLLDLTPFTKHEISGPGAEDYL
                     NKMIANRLPTKQGGTVLGHALTESGGVESEFTITRDGDKFYAVSAGAAERHDHDVLLR
                     TLPRDGSVALKNITLENGTLVVCGPRSRDLLNKISEADLSNEGFPWLTAQRITVAGVP
                     LMALRVNFVGELGWELHHPIDQQVELFDAIVEAGEEFGLKHFGMYAMESMRLEKSYRM
                     WGSDLTREYSILEAGLSRFVRLKKDEFVGKEALEKQKETGVPQEFVTLEIEATDADAM
                     GSEPIFRDGTMVGRATSGCYGHSVGKSLALGYVNAGNSDIGTGLEIEILGDRLPALVI
                     PESPCDPENEKLRA"
     gene            7370..7987
                     /locus_tag="EYO68_10850"
     CDS             7370..7987
                     /locus_tag="EYO68_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIC08340.1"
                     /translation="MPLETWLAYTLVTTTFLLIPGPTIILVISYSLLRGRQAVIALVL
                     GVGLGDLTAMSLSFLGVGVLLQTVATAFYLIKWLGAAYLIWLGIKMWCSASEFTDLDK
                     KTRSHAWREIFSSAFITTALNPKSIVFFLAFIPQFIEPELPFTTQAVILGATFFVLAI
                     ISVLGYAALAIYAGQQLHLPLIQRWTHRIGGGLLIGAGGMTAVTS"
     gene            complement(8004..8819)
                     /locus_tag="EYO68_10855"
     CDS             complement(8004..8819)
                     /locus_tag="EYO68_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HIC08341.1"
                     /translation="MINSNQLCPVLFIPHGGGPLPLLGDESHLALVSFLKEITLSLPL
                     PSSILIISAHWEEDKVTITNGKRPSLIYDYYGFPEETYQIEYPAPGDPVLANKIYKLF
                     QDSGIEAKLDEQRGFDHGMFVPLKIMFPEAEIPCVQLSLINNLDPESHFKLGKALSKL
                     RKENMLIIGSGFTFHNMQALMSQNSGVDFKNEEFEQWLIDTCTNPNYSPSLQEQKLIE
                     WSEAPFASYCQPREEHLLPLHVCAGVSGSTAKLVFEGKVAGKKTSAFLWNDFT"
     gene            complement(8837..9475)
                     /locus_tag="EYO68_10860"
     CDS             complement(8837..9475)
                     /locus_tag="EYO68_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC08342.1"
                     /translation="MKFNNSHVDLDEIEEFTIAHYEDNAESFLVGTKDHDVSQNIDAF
                     LSALPKEKNLDILDFGCGPGRDMCVFKSMGHRPVGLDGSKEFCKMAEQQSDCPTLNQQ
                     FLNLKLEEKSFDGIFANASLFHVPSKELPRVLNELHCALRKDGILFSSNPRGNAEGWQ
                     GQRYGHYMEFEASETYLQQSGFKIIEHFYRPAGMPREHQPWLAIVSQRQDPK"
     gene            complement(9499..9828)
                     /locus_tag="EYO68_10865"
     CDS             complement(9499..9828)
                     /locus_tag="EYO68_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIC08343.1"
                     /translation="MSVLAQETCIPCRGGVPPLKGEELDALQEKLGNGWQIINEHHLE
                     KEYIFADFRQALDFTVKVGEVAENQGHHPDIYLAWGKVKLTIWTHKIDGLTESDFILA
                     AKADQEL"
     gene            10017..10754
                     /locus_tag="EYO68_10870"
     CDS             10017..10754
                     /locus_tag="EYO68_10870"
                     /inference="COORDINATES: protein motif:HMM:PF01553.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIC08344.1"
                     /translation="MKARLLKRLSILLSATFYTLKTSFFAVSVSFRGNLTREITNMQL
                     NSWAKQLLELIGANYKVEYNVPIELETGRSYILMSNHQSYYDIPLLYMTIPDKANLRM
                     IAKKELFRIPLWGRGLKTAEFISIDRSDHEKSMESMEAAKEKMKSGIMIYVAPEGTRS
                     RDGKLGVFKKGGFLLAIQTESIIIPIGLRGTEGAMPPDTWDFSLGQKLEAHVGTPIDT
                     ANFSIEERDTLMQEVRTQILALSKKPE"
     gene            complement(10792..11280)
                     /locus_tag="EYO68_10875"
     CDS             complement(10792..11280)
                     /locus_tag="EYO68_10875"
                     /inference="COORDINATES: protein motif:HMM:PF03928.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HIC08345.1"
                     /translation="MKNFIGLLILAVLFLVPFEVFAAEKLPTVRLRVLASHIANKIVT
                     EAVNNCATRGYLVSAAVVDRNGNLAAFLRNPLSGPHTIKVSQRKAYSSATLRAATSQM
                     GARSDLSFAPDILLIVGGVTINFSGIFYGGVAVSGAKPDIDEECAKAGLEAVADIMDF
                     ID"
     assembly_gap    11459..11558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11560..11718)
                     /locus_tag="EYO68_10880"
     CDS             complement(11560..11718)
                     /locus_tag="EYO68_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08346.1"
                     /translation="MKPRISMITLGVNDLEKSIKFYHEGLGFPKMETSPEVAFNPFFW
                     VGPEDENE"
     gene            complement(11750..13123)
                     /locus_tag="EYO68_10885"
     CDS             complement(11750..13123)
                     /locus_tag="EYO68_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIC08347.1"
                     /translation="MPVPSEGESNSSSKRTEWEAKNHNTETRAILEEDASYFLRQSVS
                     SPCLSVVTKAEGAYIEDTNGRRYLDFHGNNVHHIGYGHPKLKKAIAEQMDALPFAPRR
                     FASESALALAKKLVEIAPGDLSKVLFTTGGSDAIEVALKISRAATGRHKTISFWDAFH
                     GAGFGAASVGGEQLFRSHVIGPLLSGTEHVAPFACYRCPYGYPDLNGEAQLEICQTTC
                     ASFVRYVLEKEGDVAAVIAEPARAVPYIPPPGYWQMVRQACNDHGALLIFDEIPTGLG
                     KTGKMFACEHDDVVPDILVLGKALGGGILPIAAVLAKTELDVAGEFAFGHYTHEKNPV
                     TAKAALTTIEIIKEEGLVENAAELGKYMLEQLNLLKAKHSTIGDVRGRGLLVGVELVL
                     NQEEKTPANDLAEDVLYRCLSKGLSFKLTMGNVMTLTPPLIIKKAQIDLSLEIIDQSL
                     SEAEQAL"
BASE COUNT         4012 a         3016 c         3184 g         3930 t
ORIGIN      
        1 tgtgaaaaaa agaaatgatc aggattggac ctatttgcct tatgggccat ttgatcgttt
       61 tgaaaattat ctaaagtgga tgaaaaatgc ctgctccggc aaagatccgc tttttcatgt
      121 gattaaagac gctattacac aaaaggctct tggagtggcc agttatctgc ggatagaacc
      181 cattttagga gtagttgaag ttggacacat tcatttttca cctgtgcttc aaaaaacacc
      241 acatgcaacg gaagcgatgt ttttgatgat gattcgggtc tttgatggac ttggttaccg
      301 tcgctatgag tggaaatgtg atgctctcaa ttcacggtcc ataaaagcgg cagagagatt
      361 gggatttaaa tttgaaggta tttttcgaca ggataaaatt tataagggac gcaatcgtga
      421 tacagcatgg ttttccatta ttgacaaaga ttggcccaac ttaaaaaacg catttcagtc
      481 atggcttaat ccggaaaatt ttgatacgga tggtcaacaa atattaagtc taacagaaat
      541 tagaaataat cagtaaaaaa catgaaagtt actgtatatc tttccggaga aattcacacc
      601 gattggcgca acgaaatcaa agatggtgcg cagaaatacg ggcttgatat tgagtttgtt
      661 tcggccgtga ctgaccatga tgccagtgat tcagccggtg atgttttagg gccggaagat
      721 agctcatttt ggcgtgatca caaatccgca aaagtgaaca acatccgcac caaaacattg
      781 atcaaaaaga gtgacttggc ggtcatccgt tttggtgaaa aatacaagca gtggaatgcc
      841 gcatttgacg caggatattg tgcagcttcg ggaacgcctt acgtaacttt acatgcagat
      901 gatattgtgc atccactgaa agaagtagat gcctcagcca tggcttggac aaaaacaact
      961 gagcaggtaa ttgaaatatt aaaatacctg acaaaagcat aaacctttga ataatagaaa
     1021 gagaatagag tgataaaaac cagcccactt tcgggaattt accggaatca ccccgtcaaa
     1081 ttgactgaag gttccggatg gcagattgct gaaaattttg gaaatgacga acgtgaatca
     1141 ctacatttaa gccatgatag tattctggta gattggtcac acattggcaa aatatccctt
     1201 tcaggagggg atgcggcaaa agctgcagaa ctgactttta aaggtgcttc gaagcttgaa
     1261 cctttaaaaa ctttgtcgaa caaggacata gcggtgttgc gtttaacagg caacgattat
     1321 ttaattttgt gccagcctgg aatggaaaca gagttactgg gaaaaattga tccaaaaatc
     1381 actacagtca tcgaccaaac cggatctcag ggctgtctcg ttttgggcgg accacgacgt
     1441 gatgaagtat tgcagcgttc aactgctgtt gatttgagac gtgataaagt agtagccggt
     1501 tcagtacttc aatcttcgat tcatacagta ggtatgactt tgtatcgtac caaaaatttt
     1561 gacatcatgc tccatcctcg aaacttatct gaatctctat acgatgcact gatggatgtt
     1621 ggcattggga taggattggt tccaggtggg attaacacgg ttccagtttc ttttgaggag
     1681 gggaaataat gactcaacga atgtggcaaa accacgatct gaaaaagaat tacgatgtag
     1741 taatcattgg cggtggtgct cacggtctgg caacagcttt ttatttggcg aaaaatcacg
     1801 gcatcaccga tgttgcggta ttggaacaga aattcatcgg ttacggtggt tcaggacgga
     1861 acaccgccat actccgctca aattaccgca cagaagaagg tataaaattt tatgaccaga
     1921 gccttgactt atatgaagat ttagcgggtg aactggattt taacctgatg ttcagtcagc
     1981 agggacattt cactcttgga cattcaactg catctatttc cggattagtt acccgtgcgg
     2041 aaaacaacaa agcacttggt atcgacagct tcatgcttga ccccaaggaa attaaaaaaa
     2101 tgctgccgga aatcgatatt acggatcatc ctcgttttcc agtacatgga gcactttatc
     2161 accctcctgg tggaataatt cgtcatgatg ctgtagtttg ggcctatgct cgaggtgcag
     2221 accggaaagg tgttcaaatt caccagatga cggaagtaca ggatattctt gttgaaaatg
     2281 gtaaagcgac cggtgtggtc acgaatcgag gtacgattaa ctgcaacaca gtaatttccg
     2341 ttgtagcagg ttggagttca gaagtttgta aaaaagttaa cgtaaaactg ccctttgtaa
     2401 cgcatcccct gcaggccctg gtcacgcagt cttacaaacc gtggctgcat catgtggtgg
     2461 tcagtggaac cctgcacatt tacctcagcc agactgatcg cggcgaactg gtgtgcggaa
     2521 atggaatcga cacctatccg cattatggta tgcgctctac tcttggcttt ttggaaagtt
     2581 atgcagcaca tgttttggaa ctcttcccgg ttttacacaa tgtcgcagtt caacggcaat
     2641 gggccggttt gtgcgacatg actccggact tcagtcctat catttctccg attgacgaaa
     2701 ttgacggttt ttatgtcaac ggtggatggg gaacttacgg ttttaaggct tctcccgcaa
     2761 gtggttacaa taccgcccaa atggtcgcca atggaaaaac tccggaaatg attgagccgt
     2821 tccgctacaa ccgttttgcc gaaaaccggc tggttggaga gaaagcggca gcatcagttg
     2881 ggagttagga ggctcaccga tcacagtttt tttattttga gacacgcctt attggatcag
     2941 tgatgctaaa agactgtggt tgttttttta atctctttta aatggaaata agtggaaata
     3001 atagtaccaa ttaaacaggt gccggatgtg gcacttaata ttaaagtcaa agacggagct
     3061 gtcgtggaag aaggcttgag ttatgtcatc agcagttggg atgaaaccgc cttggaggct
     3121 gctctgcaaa tgacagaaga cgtgggtggt gaagtgactt tgctgacagt cggaccggaa
     3181 aaggcttcag aatctttacg taaaggtttg gcaatgggcg cgcacaaagc gattcatgtc
     3241 aaatacgatt tcgctaaaga aaccgactcc tttgcatatg cgaaaatttt gcaaaaagtt
     3301 cttgaaggtc gtaactatga tctgattctt actggaaagc aggcacaaga cactgatgcc
     3361 ggcttgactg ctccaatgct ggctgagttc ctgggtttac cgcaagttac gaatattatc
     3421 cggtttagtg atgtttcaga tcagaatatc actctttacc ggcagggtga tcatgctaca
     3481 gaagttttgg aaatgaatct tccgggagtg gtaacagtca acgacagcct caacgaacca
     3541 cgattagctt cgatgcgcgg aattatgatg gccaagaaaa agccgcttga agaagtagac
     3601 ttggatgcac ttggtgtttc agccgaaatg cgtggtgcgc aggcttctat gaccgaagtc
     3661 ttacagtacg acaaacctga gtcacgtaaa gaaggtaaaa tatttgaagg cgatgaggct
     3721 gattccgtca aacaagctat ggaacttctg gtcagcgaag ctaaattttt agcctgaact
     3781 ttttttagtc aaaaacacac aagaataaaa taattctttt ttgtgggttt ttgataaata
     3841 ttattgaaaa ctaaaatcta ataataaaat gtcaaaaatt ctcatagtcg ctgaagttaa
     3901 aaacggcgaa attaagaaaa acactttgga attgttatcc ttcgccaagt cgcaaggatt
     3961 ggaaaatgag gctgtactga ttggatccgg agtatccgga caagctgata ttttagccgg
     4021 acaaggtgca tcgaccgtct atctgggtga cgatccgtcc ttggaaattt acaacactga
     4081 gcaatacacc gctttagttt ccgatgcaat tctgcaatcc ggtgcaaccc aggtttggtt
     4141 gtcctcttct gaattgggac gggacctaac cccgcgagta gctgcaagac aaagcgtggg
     4201 tgcacttagc gatgtgacgc agctggaaat cagcggtgac gaaatcacgg cctaccgtcc
     4261 atgtatgtcc accaaagtta tccaaaaatg caaattcagc aaggatggtc tgcgcgtcat
     4321 cagcatccgt agcggatttt ttacggcaga tgagacatca ccagctaccg caaaaacagt
     4381 ttcactctca ataccggaag gtcattcaaa actgaaagtc aaagaagttc aagtagaagt
     4441 gagtgatgaa gttgaactca atgaggcacg tattgtagtt tctgcaggac gtggtgtagg
     4501 tggtacagaa ggctgcgatt ttgttaagcc cttggctacc gatcttggcg cggctttcgg
     4561 tgcgtcacgg gctgtttgcg acgcaggttg gctacctcac aaacaccagg tgggccaaac
     4621 cggaacaatg gtcactccag atttttattt tgcattgggc atttccggtg caattcagca
     4681 tttagccgga atgtccggtt ccaaagtcat aattgcagtt aacaaagatc cggacgctcc
     4741 aatttttaaa gtcgctgatt acggaatcgt aggagatctt tttaaagcag ttcctgtttt
     4801 gcgtgaggaa gtacaaaaac taaaggctta gcaaaatatt gaagtagaaa aaaaataaaa
     4861 aaagtaatta tgaaatctca ttacagagcc gttgttatcg gcggaggagt cgtgggcgca
     4921 agtgcactgt atcatttggc aaaaatgggc tggaacgatg tggtccttgt cgagaaaata
     4981 gagtatacat ccggttcaac ctggcacgca gccggactgt tgccgctgtt caatatgagt
     5041 tacagcgttg ggaaaattca taaatacagc gtggatttgt accaaagttt agaggaggaa
     5101 accggacaac atgtcagttt tcacaaaacc ggaaatttgc gtttggcacg taatcaggaa
     5161 cgtatggatg agtacaaacg ctattgcgga accgcttcca cgattggcgt tccatttgaa
     5221 atgatcggtc ctgctgaaat caaaaaactc tggccgtttg ccaaagtcga tgatcttgtc
     5281 ggagcgcttt ttcatccgga tgacggacat gtcgctccgg tggatgtgac catggctttg
     5341 ctcaaaggcg caagggcgaa tggagcggaa acttattcgg aagttgaagt aaccgcaatg
     5401 gcgcaaaagc caaacgacga gtgggtcgtc agcaccaaca aaggtgacat tacctgtgat
     5461 gtggtgattt cctgcaccgg caattatgcg cgtgaaacag gacgtatggt cgggcttgaa
     5521 ttgccggtgg ttcccgtgga acatcagttt atcgtaaccg gaccgattcc ggaattggtg
     5581 gaatacaacc gagccgggaa tccggaaatg gcggttttgc gtgaaagcga cgcttcttat
     5641 tatatgcgtc aggaagcgga cggtttgatt ttgggaccgt atgaaaaagg tgccccatgc
     5701 tgggcactgg atggagttcc ggaaggcttc gagaaggagc ttttgcctcc ggatttggaa
     5761 agactggaac cgcacattat tgctgccgga gagcgagttc cgatttttga aactgccggt
     5821 atcaaggatc atatcaacgg cccaatcgcc tacactccgg acgggaatcc gatggttgga
     5881 ccggcttggg gattacgcaa tttctggctt tcggaaggac acagtttcgg cattactgca
     5941 gcaggaggtt ccggaagaca tctagcagaa tggattgttg aaggttctcc aagcatcgac
     6001 atgaatggcg ttgatccgcg ccgtttcagc gcaacgcaat ccacccgaga ttttataaaa
     6061 gccaaaaacg aagaatgcta cgaacatgtc ttcgtcattc actatgattt tgaggaacgc
     6121 actgcagcgc gtccggctaa aacgagtcaa atttatgatc gccagaaaac ccttaacgcc
     6181 gccttcggac aacgttacgg ctgggaacgc cctaactggt ttgctccgca aggctcagaa
     6241 ccgtttaacg aatacagctt tcatacccgc cgcacgaact ggtttgaaac agtaggcgaa
     6301 gaagtgcggg ctgttcgtga acaagtcgga ttgctggatt taactccgtt caccaaacac
     6361 gaaatttccg gtccaggagc agaagattat ttaaacaaaa tgattgccaa ccgcttgccc
     6421 acaaaacaag gcggcacagt tttgggacat gcactcaccg aatccggagg cgtggagtcg
     6481 gaattcacta ttacccgtga tggagataaa ttttatgcag tcagtgcagg tgccgctgag
     6541 cggcacgatc atgatgtttt attacgtaca cttccacgag acggaagtgt agcactgaaa
     6601 aacatcacac tcgaaaacgg aactttggtt gtttgcggcc cacgctcacg ggatttgctt
     6661 aacaaaattt cagaagccga tctatccaat gaaggttttc cgtggcttac cgcacaacgc
     6721 attacggtgg ccggagtacc gcttatggcc ttgcgtgtaa attttgtcgg tgagcttggc
     6781 tgggaattac accatccaat tgatcagcag gttgaactgt ttgacgcaat agtagaagcc
     6841 ggtgaagaat tcggcttaaa gcattttggt atgtatgcga tggaatcgat gcgtctcgaa
     6901 aaatcgtacc gcatgtgggg ttctgatttg acccgcgaat actccatttt ggaagcagga
     6961 ttgagccgtt ttgtgaggct caaaaaagat gagtttgttg gtaaagaagc actggaaaaa
     7021 caaaaagaaa ccggagttcc acaagaattc gtcacattgg aaattgaagc tacagatgca
     7081 gatgcaatgg gaagcgaacc aatattccgt gatggaacga tggtgggccg cgcaacttca
     7141 ggatgttacg gacacagtgt aggaaaaagc ctggctttgg gttatgtaaa tgccggaaac
     7201 agtgacatcg gtacagggtt ggaaatcgaa attcttggtg atcgacttcc ggcactggtt
     7261 attcctgaat ctccctgtga tccagagaat gaaaagttgc gagcctaggt tttcaggagc
     7321 actactattt tcgtagcttt ctcgactctt tccccaaata tataaaaaca tgcccctgga
     7381 aacttggctg gcctacactt tggtcacaac aacatttctg ttgattcccg gaccaactat
     7441 tattttggtg attagctact cacttttacg gggaagacaa gccgttatag ccttggtttt
     7501 gggtgttggt ttgggagacc ttacggcaat gagtctttca tttttagggg tgggggtgtt
     7561 gctgcagact gtcgcaactg cattttattt aatcaaatgg ctgggagcag catatttgat
     7621 ttggttggga attaaaatgt ggtgcagtgc ttcagagttc actgatctcg ataaaaaaac
     7681 caggagtcat gcttggcgag aaattttcag cagtgctttt attactactg cactcaatcc
     7741 taaaagcatc gtcttctttc tggctttcat tccgcagttc atcgaaccgg aattaccttt
     7801 tacaacccag gcggttatcc tgggagcaac attttttgta ctggcaatca tatctgtact
     7861 cggctatgcc gcactcgcaa tctatgcagg acaacagtta catctacctt taatccagcg
     7921 ctggacacac cgaataggcg gcggattgct gattggtgcc ggtggtatga ctgctgtcac
     7981 ctcatgaagc tttcattcat acattaagta aaatcattcc acaaaaacgc actcgttttt
     8041 ttacctgcta ccttcccttc aaatacaagt tttgcagttg aaccagatac ccctgcacaa
     8101 acatgcaagg gcagtaagtg ttcctctctt ggctgacaat agctggcaaa aggagcttcg
     8161 ctccactcaa tcaatttttg ttcctgcaaa cttggagaat aatttggatt tgtgcaagta
     8221 tctatcaacc actgttcaaa ttcctcattc ttgaaatcaa caccagagtt ttgagacatt
     8281 aaagcctgca tgttatgaaa tgtaaatcct gagccaataa ttaacatatt ttcttttctt
     8341 aattttgaaa gcgcctttcc aagtttaaaa tgagattcag gatctaaatt atttatcagt
     8401 gataactgga cacacggaat ttctgcttcc ggaaacatta tttttaatgg tacaaacatc
     8461 ccatgatcaa aacctcgctg ctcatctaat ttcgcttcaa tgccactatc ctgaaataat
     8521 ttatatattt tatttgctaa aacaggatct cccggagcag gatattcaat ctgatacgtt
     8581 tcttccggaa aaccgtaata atcatatatt aatgacggtc ttttaccatt tgtaattgtt
     8641 actttgtctt cttcccagtg ggcactgatt atcaggatgg atgatggtaa agggagcgat
     8701 agagttattt cttttagaaa actgaccaat gcaagatgag actcgtctcc gagcagaggc
     8761 aagggtccac caccatgcgg aataaacaaa acaggacaaa gctgattact atttatcatc
     8821 tttcactttt taattattac tttgggtcct gacgctgact gacaatagcc agccatggct
     8881 ggtgctccct tggcatccct gcaggtcggt agaaatgctc aattattttg aatccggatt
     8941 gctgcagata agtctcactt gcctcaaatt ccatgtaatg cccgtaacgc tgaccctgcc
     9001 agccctctgc attacctctt ggattggaag aaaataaaat cccatctttt cttaaagcac
     9061 aatgtaattc atttaaaacc cttggcagtt ctttactggg cacatgaaac aaagaagcat
     9121 tagcaaaaat tccatcaaaa cttttttctt ccagtttaag atttagaaat tgttgattaa
     9181 gagtcgggca gtcactctgt tgttccgcca ttttgcaaaa ctctttgctg ccatccaaac
     9241 caacaggcct gtgtcccata gatttaaaaa cacacatgtc acggcctggt ccacatccaa
     9301 aatcaaggat atccaaattt ttttctttgg gaagagcact aagaaaggca tctatatttt
     9361 gactgacgtc atgatccttc gtccccacta gaaaagattc cgcgttgtcc tcatagtgag
     9421 caattgtgaa ttcctctatt tcatcgagat caacatgaga attattaaat ttcattgatg
     9481 ttaattaata atttttgctt aaagttcctg atctgctttt gctgcaagaa taaaatcact
     9541 ttctgtcaat ccatctattt tgtgggtcca gattgtcaat ttaactttac cccatgctaa
     9601 ataaatatca ggatgatgac cttgattttc agccacttct ccgactttga cagtaaaatc
     9661 cagtgcctgt ctaaaatcag caaatatata ttctttttcc aaatgatgtt catttatgat
     9721 ttgccagcca tttcccaatt tttcctgcaa agcatccagt tcttctcctt tcaaaggagg
     9781 aacaccaccc ctgcagggaa tacaagtttc ttgtgccaat acgctcattt tatgctccag
     9841 ttcaagttat aggtttttaa tcgaaaataa attgtgagta attgtttaca gacgtattct
     9901 atcacatttg atcatattat atgtgacttt atgaaatatt cgataagttt cattcaaagc
     9961 tcatttttag ctaaatccag cttgagaata actgcctgtt gagtgatgat ttagaaatga
    10021 aagcacgctt actcaaaaga ctatccattc tattatctgc aacattctat actctgaaga
    10081 caagcttttt cgcagtttca gtttctttcc gggggaattt aacaagagaa ataactaata
    10141 tgcagttaaa ctcttgggcg aagcaattac ttgaattgat aggagcaaat tacaaagtgg
    10201 agtacaacgt gcctattgag ctggaaactg gtcgcagcta tattttgatg agcaaccatc
    10261 aaagttatta tgatattcca ttgttgtaca tgactattcc tgacaaagct aatttgcgca
    10321 tgattgcaaa aaaagaatta ttcaggatac cgctctgggg aagagggttg aaaacggcag
    10381 agttcatttc gatcgaccgt tcagatcatg aaaaatctat ggaatcaatg gaagctgcca
    10441 aagaaaaaat gaagagtggc attatgattt acgtagctcc ggaaggcaca agaagcaggg
    10501 acggtaaact gggcgtattc aaaaaaggag gatttctgct cgcaatacaa actgaatcga
    10561 ttatcattcc aatcggcttg cgtggaacag aaggagctat gcctcctgac acctgggact
    10621 ttagtttggg gcaaaaactt gaggcgcacg tgggtacccc aatagatact gccaatttta
    10681 gtatcgaaga acgggatact cttatgcagg aagttagaac tcagatactt gctctttcta
    10741 aaaaaccgga ataaatattt ggttaaattt ttttcaagac cattttttta ctcagtcgat
    10801 aaaatccata atgtcagcaa ctgcctctaa tcctgctttt gcacattcct catctatatc
    10861 aggcttcgca cctgaaactg caacacctcc ataaaagatg cctgaaaaat tgattgtaac
    10921 tccaccgaca attaatagaa tatccggtgc aaaactgagg tcacttctgg ctcccatttg
    10981 tgaggtggct gctcgaagtg ttgcagaaga gtatgccttg cgctgactca ctttaatcgt
    11041 gtgcggccca gaaagcggat ttcgcaaaaa agcggcaaga ttaccgttac ggtcaacgac
    11101 tgctgccgaa accagataac cccttgtagc acaattattt actgcttcag tcacaatttt
    11161 atttgcaatg tgggaggcaa gtacgcgcag ccgaacagtg ggtaactttt ctgcagcaaa
    11221 tacctcaaac ggaaccagaa acaagactgc taatatcaat agaccaataa aatttttcat
    11281 aacctatacc tcatttttgc tttatcaata attttaccga tcgcccgatt gttaactctt
    11341 caaataaact gctaaaaaag caggggacag tattccaatc agtcgtcaga atttaattaa
    11401 tttgtcattc attttcatct tcaggtccta cccagaaaaa tgggttgaag gcaacttcnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cattcatttt catcttcagg
    11581 tcctacccag aaaaatgggt tgaaggcaac ttcaggagat gtttccattt tgggaaagcc
    11641 caacccttca tggtagaatt tgattgattt ttccaaatca ttcacaccca aagtaatcat
    11701 acttattctt ggcttcattt tttctttcca tttttatatt cacggtactt caaagagcct
    11761 gttcggcttc actaaggctt tgatcaatga tttccaatga aagatcaatt tgtgcctttt
    11821 taatgatcag cggtggtgtg agcgtcatca cattacccat tgtaagctta aatgacaagc
    11881 ctttgctcag gcagcgataa agcacatctt cagctaaatc gttagcagga gttttttctt
    11941 cctgattgag tacaagttca actccaacca gtaatcctcg tccgcgtacg tctcctatag
    12001 ttgagtgttt tgctttcaac agattgagtt gctccagcat gtattttcca agttctgctg
    12061 cattttcaac caaaccctcc tctttaataa tttcaatagt cgtcaaggcc gcttttgcgg
    12121 tcaccggatt tttctcatga gtgtaatgac caaaggcaaa ctcaccggca acatcaagtt
    12181 cagttttggc gagaaccgca gcaattggta aaataccacc gccaagcgct tttcccaaaa
    12241 ccagtatatc cggtacaaca tcatcatgtt cgcaggcaaa cattttcccg gtcttgccaa
    12301 gaccagtcgg aatctcatca aagatcaata aggcaccatg gtcattgcag gcctgccgca
    12361 ccatttgcca gtatcctggt ggcggtatat atggaacagc cctcgccggt tcggcaatca
    12421 ctgcagccac atctccttcc ttttccagta catagcgcac gaaactggca caggtcgtct
    12481 gacagatttc aagttgcgct tctccattaa gatcggggta accgtaggga caacggtaac
    12541 aggcaaaagg cgctacatgc tctgtgccgg aaagtaaagg accaataacg tgtgagcgga
    12601 aaagctgttc tccgccaaca gaagcggctc caaatcctgc tccatgaaag gcatcccaaa
    12661 atgaaattgt tttgtgccga ccggtcgccg ctcgtgaaat tttcagtgcc acttcaatcg
    12721 catccgatcc tcctgtcgtg aataagacct tggaaagatc acctggagca atttcaacca
    12781 gtttctttgc aagcgcaagt gcggattcac tggcaaatct tcgcggtgca aaaggcagag
    12841 catccatttg ttctgcaatc gcttttttca gtttgggatg accgtagccg atgtgatgca
    12901 cattattgcc gtgaaaatca agataacgac gaccatttgt gtcttcgatg taagctccct
    12961 cggccttggt taccacagat aagcatgggc tggaaacgga ttgtctgagg aagtaactgg
    13021 catcctcctc aaggattgct ctcgtttcag tattatggtt ttttgcctcc cattccgtac
    13081 gcttacttga actgttgctt tctccctcag aaggtactgg catccgattc ttgaaattag
    13141 tcattgtttg ctagtcttga gtaaggttta gttgaatttc cagcacttag ttcttcggct
    13201 acccttacca ccacaaacga ctcgtcttga aaccagagac caccgtgacg ttggagaacg
    13261 tttttggtcg cttgcagata atcgcccgat tggagcattt cattaagccg tacatcttcg
    13321 atttgtgcaa cgtacacagc cgcgttccag gctgccaaca atgtagatgt accggtattg
    13381 ctgccgattt catccggcaa ggcgtgcagg tggtaggtaa ataaagaccg ttcatcgtta
    13441 aagccttcaa aggcaaaata ctccttttcc atatttagat cttgttgcag ttctttgatg
    13501 agcaaatggc ggggcgtcac aaacggctct tcgtccggcc acaattttcg aataatttcc
    13561 atgcccggat cgtgtcctgt cgattggaca acaaccatcc ggcctccgac attgagactt
    13621 ttagcgagtg gggcgagtac tttttctact ttgaaatcgg ctgacattcg actccggtac
    13681 ggctgagcag ccaccactaa atcatagttg ccttcatacc gtcccttgcc cggaatgatg
    13741 ctcgcaagcg ggaaagcctg gtcttggcgg tagagtacca taacagaggg cttgacatag
    13801 agtgggttgc ctgacttgag actggatttt gtttgccagc cacgctggag ctgcatatca
    13861 agctgtgtaa ttcgtttgct gaattcgtgt acggtggttc cttcaagcgg aacgtcccac
    13921 cacagttgtt gatctctggc tgatttttgt gctggttcaa gccatggtgc ttcggcgtag
    13981 taaaggttgg tgagaatcac caccatttgt ggatgttcgt aaaagcggtc ggctaatttt
    14041 tcaaggcaaa ggcgggtgtc ctcaaggctg atttcttttc ccacaacaaa gaaaggtaca
    14101 gaggggaaac gacagtgcat ttcgcacagg atgcggctaa gaaccatgcc atttcccacc
    14161 cccgcatcaa aaattttcag ggccggcggc ttaggcttta tccaatcaaa ttctcgacca
    14221 acatgttcgg cagtaatcgc tt
//
LOCUS       DTVD01000299            3767 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_9068_length_3767_cov_346.558190, whole genome
            shotgun sequence.
ACCESSION   DTVD01000299 DTVD01000000
VERSION     DTVD01000299.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_3767_cov_346.558190"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="EYO68_10890"
     CDS             <1..127
                     /locus_tag="EYO68_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIC08348.1"
                     /translation="GAIISGGHGTAAEKMSALQEAGIHVVKTPAEIGEKMLEALA"
     gene            complement(248..664)
                     /locus_tag="EYO68_10895"
     CDS             complement(248..664)
                     /locus_tag="EYO68_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08349.1"
                     /translation="MQLLVNMLQGRMLEHIKQRVSSYYHIEPEALNEEFSVSLIEVFA
                     EIFGLFRNKFEEMPWLVNEIAKRIVEVESRNGSKTERHINQLYLSIFCKHFEYKNIEK
                     IISTLQTDPRIQNAIFTALPTAAHSPHNFRPAIASN"
     gene            complement(1265..2116)
                     /locus_tag="EYO68_10900"
     CDS             complement(1265..2116)
                     /locus_tag="EYO68_10900"
                     /inference="COORDINATES: protein motif:HMM:PF03372.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08350.1"
                     /translation="MLTIVSWNIQYGKGVDGHIDLSRIAREILIDGFPDLICLQEVSR
                     NYPATDNGSDQVAELQKFFPEYESFFGASHDRSGGVNGGRRQFGNLVLTRHSPIQVLH
                     HLLPSPADPKVKFMSRQTTELVIPTSSGAFRMMNTHLEFFSEKQQLSQVQRIRELHEE
                     ACIQYNEPGLDLPNTPFERVDRPEKMVICGDFNFTLDSVSYQRMTAPFPDKIPDLFDA
                     WNVVNPDGNRSPTCGVFDHKQWKNGPHCRDYFFLSENFLQQIQNVSVNTDTNASDHQP
                     IRLTLKV"
     gene            complement(2123..3610)
                     /gene="mtaB"
                     /locus_tag="EYO68_10905"
     CDS             complement(2123..3610)
                     /gene="mtaB"
                     /locus_tag="EYO68_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIC08351.1"
                     /translation="MSLLSETALPPDSETFTADELNEGQEPAKKRVSFHTLGCRLNQS
                     ETGVLARSFEQQGYAVVPDYARAEVYVLNTCTVTEHSDAKNRQIIRKLHRQNPEAIIA
                     VVGCYAQIDPEAIAKIEGVSLVIGSEEKMRITDYLAQIDSQNTVLIVRPKINKTTFTA
                     PVIPHSSTLSTQKLRKQTKTIERDVVPANSTLQFSGTTTSHQSTRTSLKIQDGCDFMC
                     AFCIIPFARGRSRYREFSNLQEEARMLVQEGIREIVITGVNVGTYKTGKRTLVDVVDF
                     LNTLPGLSRIRISSIEPTTVPELLFQYMSDPQHKLVPFFHLPLQSGSDIILQKMKRRY
                     SSAEYTDEIWRAFESVPDLCIGTDVMVGFPEESETEFKSTLKLLKKLPLAYFHVFPFS
                     ARNGTPAFKMKEQVPPTVKQQRGEKLRLLSTKKRRVFHQKFLGQSREVLWESMKPDGT
                     ISGYTDNYIRVVLNELYNSDLRNQLLPVRLKELKGQTILGKFESG"
BASE COUNT         1051 a          756 c          796 g         1164 t
ORIGIN      
        1 cggtgccatt atttcaggtg gacatggtac ggctgctgaa aaaatgagtg ctttgcagga
       61 ggccggcatc cacgtagtga aaactccggc agaaattggc gaaaagatgc tggaagcttt
      121 agcttaaatc ttggttattc gtcacacaaa gctctgagca tcaaatgttt tctgttcctc
      181 agaatggaca caatagtttg tcagagctaa attgaaaaat ttctcagagc aatctgaaaa
      241 actttgatca gttacttgca atagctggtc taaaattatg aggagagtgt gcggcagtgg
      301 gaagtgcggt aaaaatcgcg ttttggattc gcggatctgt ttgcagggtg gagattattt
      361 tttcgatgtt tttatattcg aaatgcttgc agaaaatgga gaggtaaagt tgattgatgt
      421 gcctttctgt ttttgaacca ttccttgatt caacttcaac tatacgcttt gcgatctcgt
      481 ttaccagcca tggcatttct tcaaatttat tccgaaacag accaaaaatt tctgcaaaaa
      541 cctcaatcaa cgaaacagag aactcttcat tcagtgcctc tggttctata tggtagtagc
      601 tgctgactcg ctgttttatg tgttccagca ttctgccctg gagcatgttt accagcagtt
      661 gcatttcctg ctttacgttg cagtttgatt cttccgaact ggccttggaa agtgaatcta
      721 aacaatcagc aataacccga gtatacgggc ttatagttgt catttttaac agaagaccaa
      781 agaaaaccag tgagatggtt atcttagagt gctatgtccg ttacagtgtt aataataagt
      841 aagcaagcag ataactgatt tcaatgctta aaagtcaatc tatatacact atgcagcttg
      901 ctaataaatt acaagtccat tgggaaaaat tttctatata tttttcctgt ccagtattgt
      961 gcaaaccgca caccattatt tttctgccat ttccatcaat taattttgct ccgttcaata
     1021 cctgattttt tagtattatc agtagcactc tattatcatg tcaggtcttt cttcccagcg
     1081 agcaactttt aaaacacgaa aacctctgtt acaaattaga tctgatttgc ggtagcagta
     1141 aaaaacatgt ggcggacaag aaacgtagta gcctctggat gatctgggtg tatccttgag
     1201 agatactgta cctgaacatc ctataataat tgtattcaaa agatggaata tccagcgtga
     1261 catttcaaac ctttaatgtt aagcggatcg gctggtgatc tgaggcattt gtatcggtgt
     1321 tgacagaaac gttttgaatt tgctgaagaa aattttcgct gaggaaaaaa taatcacggc
     1381 agtgcggacc gtttttccat tgtttgtgat caaacactcc acaggttgga ctcctgttcc
     1441 catcaggatt cactacattc caggcgtcaa acaaatccgg aattttgtcg ggaaacggag
     1501 cggtcattcg ctgataagaa acactgtcca gtgtgaaatt aaaatcaccg caaatcacca
     1561 ttttttctgg acgatcaact ctctcaaacg gagtattcgg taaatccagt ccaggttcat
     1621 tatattgaat acaggcttct tcgtgcaact cacggatacg ctggacttgt gacaattgtt
     1681 gtttttctga aaaaaattct agatgtgtat tcatcatccg aaatgctccg gaactggtgg
     1741 gaatcacaag ttctgttgtt tgcctcgaca taaattttac ttttggatca gcaggagagg
     1801 gcaacaggtg atgcagaact tgaataggtg aatgccgggt cagcaccaag ttgccaaatt
     1861 gtcgccgacc tccgtttact cctcctgaac ggtcatgtga cgctccaaaa aaagattcgt
     1921 attccggaaa aaatttctgc aattctgcaa cctgatcact cccgttgtcg gtggcaggat
     1981 agtttctgga aacttcctgt aaacaaatta aatccggaaa tccgtcaatg aggatttccc
     2041 gggcaattcg gctcaagtcg atgtgtccat ctactccttt gccatactga atattccaac
     2101 ttacaatagt taacatatgt ttttaacctg attcaaactt tcccagtatc gtttgccctt
     2161 taagctcttt caatcggaca ggaagaagct gatttcgtaa atcagaatta tataattcat
     2221 tcagtactac acggatataa ttgtctgtgt atccactgat agtgccatcc ggcttcatgg
     2281 attcccaaag tacttctcgt gattgtccta aaaatttctg atgaaataca cgacgttttt
     2341 ttgtgcttaa caaacgcaac ttttcacctc gttgctgttt gacagttggg ggaacttgtt
     2401 ctttcatctt gaatgccggt gttccattac gggctgaaaa tgggaaaaca tgaaaatatg
     2461 ctagaggcag ttttttaagt aatttgagag tgcttttaaa ttcagtttca ctttcttcag
     2521 gaaaccccac catcacatct gtaccgatgc acaagtccgg gacactttcg aatgctcgcc
     2581 atatttcatc tgtatattct gcggaagaat aacggcgctt catcttttgc agaataatgt
     2641 cagatccgga ttgtaaaggc agatgaaaaa acggaaccag tttatgttgc ggatctgaca
     2701 tatattgaaa aagtaattcc ggaactgtgg ttggttcgat ggaactgatc cggatccggg
     2761 acagtcccgg aagagtgttg agaaaatcaa ctacgtccac taaagtccgt tttccagttt
     2821 tatatgttcc aacgttgact ccggtaatta caatttcccg tatgccctct tgaactagca
     2881 tcctggcttc ttcctgcaaa ttcgagaatt ctcggtaacg ggatcgacct cttgcaaaag
     2941 gtatgatgca gaatgcacac atgaaatcgc agccgtcttg tatttttagc gaagtacgag
     3001 tgctttgatg agaggttgtg gtaccagaga attgaagcgt tgagttagcg gggaccacgt
     3061 cacgttcaat tgttttagtt tgttttcgca gtttttgggt agataaagtt gagctgtgag
     3121 gaattacagg agcagtgaaa gtcgttttgt ttattttggg ccggacgatt aaaactgtgt
     3181 tttgggaatc gatttgagcc aaataatctg ttatccgcat tttttcttca ctgccgatta
     3241 ccagactaac cccttcaatt tttgcgatgg cttccggatc aatttgcgca taacagccga
     3301 caacggcgat aatcgcctcc ggattttgac ggtgaagttt acgaattatt tgacggtttt
     3361 tggcatcact gtgctctgta accgtgcagg tatttagtac gtaaacctct gcccgagcgt
     3421 agtcaggaac cacagcataa ccctgctgct caaagctgcg ggctaatact cctgtttcgg
     3481 actgatttaa gcggcatcca agcgtatgaa aagatacccg tttttttgct ggttcctgac
     3541 cttcgtttaa ttcgtctgca gtgaaagttt cagaatcagg tggtagagcc gtttcagata
     3601 atagagacat ttggcgggtt aaaagtggac tggggaatat cttcctgtta atataatcat
     3661 aatatttgtc gaactcaaaa tcaaaggatt tttgtgttta gatgccctta ctctcccata
     3721 acgaggcgaa attttaaaga atgatagaat ttaacacgca cccggac
//
LOCUS       DTVD01000300           13987 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_926_length_13987_cov_259.672194, whole genome
            shotgun sequence.
ACCESSION   DTVD01000300 DTVD01000000
VERSION     DTVD01000300.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13987)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13987)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13987
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_926_length_13987_cov_259.672194"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            188..1459
                     /locus_tag="EYO68_10910"
     CDS             188..1459
                     /locus_tag="EYO68_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC08352.1"
                     /translation="MRIKKLALTALTLFFSFSLYAAETIKIGASMPLTGMFAVPGSKH
                     LEGYQMCVDIINQNGGWLGKDVILLAIDNRSDTETSISQYERFINVDKVDLVFGTFSS
                     KLTFPTSTILAKYNYVLPIPSGGALRIYRRGFKNLFYFQSNPAEYYGKQLLAVVRDRA
                     SASDQPKTVALVHADDFFANSIANGLLGKKVKLPGSGKLVEDMAPGYIVEAGMKLVYH
                     EQWPEEGFSDWLTLANSIKNSGAEMVIGMMASPEEAVQLSRALKTVRHNPKHLMLTQG
                     TQSEFLEGLGDATEGIMIYAAWHDDAPYEGTLAGQRMNNSDFVAKYKAMNGKLPDEDV
                     AITFALCQGIDQAVNATRSVDNARIMSWLHTRTSRNPVKTILGDYYWDSTGLPINKQA
                     LIVQWQGSKLKFIYPTNEFQASSMISPKPAW"
     gene            1644..1856
                     /locus_tag="EYO68_10915"
     CDS             1644..1856
                     /locus_tag="EYO68_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08353.1"
                     /translation="MILIEEEGTEFNVNSSYENQDEIDRVVLGSDSCWNKSKERQIEI
                     ATKHKGLSGDDQALILNTTSKCLLGI"
     gene            1875..2342
                     /locus_tag="EYO68_10920"
     CDS             1875..2342
                     /locus_tag="EYO68_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIC08354.1"
                     /translation="MPKVSLNVNGKVHHLDVDEKTPLIYVLRNDLGLKGTKLGCGLEQ
                     CGACKVIVDGKAEFSCNTPVEAFEKSSITTIEGIGTPDKLHVIQQAFVTECAAQCGYC
                     IPGIIVAIKALLDVNPNPDDEMICEALSDNLCRCGTHGQILKAVKRAAKEISL"
     gene            2339..4480
                     /locus_tag="EYO68_10925"
     CDS             2339..4480
                     /locus_tag="EYO68_10925"
                     /inference="COORDINATES: protein motif:HMM:PF02738.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIC08355.1"
                     /translation="MTLSSSFGNNPDLDSWLQFHRDGRITVYTGKVELGQKLTTALSV
                     IVAEELDVDPGRITVLTADTDKSPNEGYTAGSNSLESSGIALRQASAKARRALMEKAS
                     EHLEEPVENLSVTDGEIGGATTNKNVTYWDLIGDKHFKITVTDDVLPKSPDQYHLIGN
                     PVPAVGLEALVTGTMTYVHDMEFTDMAHARVIRPPNYHAQIQDLNDTEVRAMPGVIEV
                     ILDGSFLSVVCEREEQAIWAASKLARITVWDESKELNSKDIFVQLKNNPRQSLPVVEG
                     VAKESPVQPFRPPENSSKTLSATYQRPYHMHGSIGPSAAIALYDAQVLKVWTHSQGIF
                     PLRLALADVLGLAEDKIHLVHVPGAGCYGHNGADDVALDAALAARAVPGRPIMLKWER
                     ADEHAWEPFGSAMQIELQASLDDTGRVIYWNHETFSDTHMSRPSAHRENSRLLAAWHL
                     QSPRPAPPVKPTPGFHTGIHRNADPIYDFADRRVVKHLVHDLPLRVSALRSLGAYANV
                     FAIESFMDELAHFRDMDPLEFRLAHLSDNRARAVLEAATSKANSGFSSADDGHGRGLA
                     VARYKNSKCYVAVVIDLIVDDYGAIQLLRAVIAADAGQIVDPDGLRNQLEGGLMQSAS
                     WTLKEQVNFDDKGIVSRDWESYPILTFAEVPEIETVLIDHQDCPFLGSGEATQGPTAA
                     AIGNAIFDSVGLRLRRIPFTQDRVRDAAAEV"
     gene            4778..6049
                     /locus_tag="EYO68_10930"
     CDS             4778..6049
                     /locus_tag="EYO68_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIC08356.1"
                     /translation="MRIKKLALTALTLFFSFSLYAAETIKIGASMPLTGMFAVPGSKH
                     LEGYQMCVDIINQNGGWLGKDVILLAIDNRSDTETSISQYERFINVDKVDLVFGTFSS
                     KLTFPTSTILAKYNYVLPIPSGGALRIYRRGFKNLFYFQSNPAEYYGKQLLAVVRDRA
                     SASDQPKTVALVHADDFFANSIANGLLGKKVKLPGSGKLVEDMAPGYIVEAGMKLVYH
                     EQWPEEGFSDWLTLANSIKNSGAEMVIGMMASPEEAVQLSRALKTVRHNPKHLMLTQG
                     TQSEFLEGLGDATEGIMIYAAWHDDAPYEGTLAGQRMNNSDFVAKYKAMNGKLPDEDV
                     AITFALCQGIDQAVNATRSVDNARIMSWLHTRTSRNPVKTILGDYYWDSTGLPINKQA
                     LIVQWQGSKLKFIYPTNEFQASSMISPKPAW"
     gene            6171..6731
                     /locus_tag="EYO68_10935"
     CDS             6171..6731
                     /locus_tag="EYO68_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIC08357.1"
                     /translation="MPKLRIKYISPDKVKDQKILDAITDSVIKGTPRAESQAIRARVP
                     RVFWSFQNTWKDVFLEGKVDHNLKELCRVYVSKSVQCKYCGNQRSSKSAYDGLVEDDY
                     RDLLNFEKSEKYDEPTKAALALSQAITWDLETDDEFWNRLYRYFSEEEIIELGYFVGF
                     TMGQQRFNRLLNLHEHLGKTGFENMN"
     gene            6803..7534
                     /locus_tag="EYO68_10940"
     CDS             6803..7534
                     /locus_tag="EYO68_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08358.1"
                     /translation="MLKINNATKSFAGIVAVDNCSFAIKQGTISALIGPNGAGKTTLF
                     NCISRIYPIDSGQIFYKNHRLDLMPAHKIARLGIGRSFQITRTLDQLSVTENLALHTA
                     GGIGSHLFHPGITTENRLKACEVMEFLGISTLADVDMGTLSFGQKKLLDLGTVLMADP
                     DLILLDEPAAGINLRLLEDIINKLVTLKNQGKTILLIEHNMDLVMGISDEVIVMSAGI
                     IIAQGKPSEIQKNAKVLEAYLGSKA"
     gene            7535..8263
                     /locus_tag="EYO68_10945"
     CDS             7535..8263
                     /locus_tag="EYO68_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08359.1"
                     /translation="MNSLLNINNLTAGYGYGPDIVRDFSLTLNKSEVKCIIGPNGAGK
                     STLLKSIAGILKPRSGEIFFDGEKISEKEAYQIMRLGICFLPQERAIFPNLTVADNLR
                     MCAYSIKDKKVAEARIKEAIEMFPVLGQRLKQIAGTMSGGQQQQLVYARAFTIKPKII
                     MIDEPSLGLAPSLVEQTFNHITQIASSGIAVILVEQNAIRGLETAGYGVVMDLGKLVF
                     EKPAKEVLQDQSLRELYLGKALKK"
     gene            8260..9132
                     /locus_tag="EYO68_10950"
     CDS             8260..9132
                     /locus_tag="EYO68_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08360.1"
                     /translation="MIQFLQVLILGLCLGFVYALMVSGLTLIFGVMRIVNLAHPILII
                     SGSFVSYWLFILIGLDPILSIPIAGLVVAALGVVIYKLIFERNAAQQTYSEMTVLLTF
                     GTAMVIDGILSFVFHNTQRTTSPSYATDAIFIGDIFIPLAQLYSGLVSSVIIVGLAVF
                     LKYSDFGIAIRATSQNRTSAELVGVNVKFVCLISFGIGCALAGAAGALVSFIFSFFPS
                     RHWEWIAIMMSLVVLGGMGSLKGAVIGAIVLSVVSSMVGAFISPAWSTMVFFLSLFLI
                     LIIRPRGLFGIQSD"
     gene            9152..10141
                     /locus_tag="EYO68_10955"
     CDS             9152..10141
                     /locus_tag="EYO68_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08361.1"
                     /translation="MRILKEKGSFLLNNKKIIPFLILIVLTGLPIIQFFGNYNYLIHI
                     VLFSFLYITMSSSWNIIGGYGGFISLGHNVFFGIGAYFSGFIFVRYGISPFITALIAG
                     LVAAVFGFVVGLITLRVKGPSYIISSIALLMIFRILFDRWELIGGTHGMTLPHNTFPV
                     EWSKIPHYYALLLLMVLSIYLSWYIRHSKFGLALRSISHDEIKSAVTGINVKIIKVTA
                     YALSAYFIGAAGAIWGDYLTYVRPNIFFIILIAANMVLMCILGGKGTVTGPIIGTIVM
                     IFFNEFILAYFGATELNIFFTGFLMIITLMYFPEGIVGSLKKRKLLPGFLDWG"
     gene            10144..11211
                     /locus_tag="EYO68_10960"
     CDS             10144..11211
                     /locus_tag="EYO68_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIC08362.1"
                     /translation="MNQKDPRVEAAKMHWSGRFVTNGVPLSVFQDVTESVNHWDHWCS
                     AWSEKASIFEALGQEAMDQGYLLSAAQHLTTAAVCYHFGKFLFVQDKEQMRTAHAKAV
                     ECRNLALPHLDPPGERVKIPFGEHCFYGNLRKPVDSIKPPVILMTLGLDSAKEEMETN
                     EIIFLERGMATLAFDGPGQGESEYEQPICPEYELPVKSVLDWLEKRDDVDSDSVGIWG
                     VSLGGYYAPRAAAFDDRIKACISLTGPFNMNECYEKLPGLTKTAFMHRSNSSNEEEAR
                     QVAVRMSLEGVAEKISCPLYVVAGKLDRVIPHQQAIKLAHTASGEVVLNLVEDGGHVA
                     NNRAYKYRTQSADWIATKLGV"
     gene            complement(11254..12072)
                     /locus_tag="EYO68_10965"
     CDS             complement(11254..12072)
                     /locus_tag="EYO68_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HIC08363.1"
                     /translation="MIKAEQMSLNTATVKEQCNLRQAIDLCARFGIGGIAPWRENLKE
                     CGLDEANRMIQDRGLRVSGLCRGGMFTLENQLSQNILDNNKKAVDEAVAIHAECLVMV
                     VGGLKPNSKDLIGSRKQMEEGLFKTLEYARTVKMPIAIEPLHPMYAADRACVNTLKYA
                     LDICDGMGAGIGIAVDVYHTWWDPELKSQIKRAGKERLLAFHLCDWLMETKHFLLDRG
                     MMGDGVIDISRIRKWMESESFEMLNEVEIFSAENWWKRDPDEVIRICIERLAVC"
     gene            complement(12069..13229)
                     /locus_tag="EYO68_10970"
     CDS             complement(12069..13229)
                     /locus_tag="EYO68_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIC08364.1"
                     /translation="MPSLLLPKPDKTLETLELSTPRELSVVPSVFPRTVYAAAHVVVD
                     PLKNYDPWLDTNIDWDQTIAFREYLWGKGLGVAEAMDTAQRGMGLDWVASQELIRRSI
                     DAANSCKGLIACGAGTDHLIPKPNLTIEEVIQAYEEQCEVVESLGGKIILMASRALTA
                     CARGPEDYATVYNRILGQVSQPVIIHWLGEMFDQELKGYWGSTSHDEAMETCLSILQI
                     NSSKINGIKISLLSKEKEIQMRRRLPKNIKMYTGDDFNYPELILGDEFGYSDALLGIF
                     DAIAGLAAAAVTALGMGDHNTYTDLFEPTVPLSRHIFKSPTRFYKTGVVFLAYLNGFQ
                     NHFTMLGGQESTRSTLHLAELIRLANQAGALLDPELAVFRGKYVLASRGVGS"
     gene            complement(13233..>13987)
                     /locus_tag="EYO68_10975"
     CDS             complement(13233..>13987)
                     /locus_tag="EYO68_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIC08365.1"
                     /translation="VKNGVVHDKLYLPGLLKLKRLRDSGFFGRILSVRGEFGYWVFDG
                     EMIPAQRPSWNYRKNDGGGIIKDMLCHWRYVLDNVVAPVKSVSCTGQVNIVERWDEAG
                     QKYISDADDAAYALFELEGGIIAQINSSWCVRVKREDLVTFQVDGTLGSAVAGLHNCS
                     TQHHVNTPKPTWNPDQPQNINYFDHWEPVPDNTEFKNGFQSQWEEFLRHVVEDGPWKY
                     DLYEGAKGVQLAECALQSWKERRWVDVPEIEV"
BASE COUNT         3893 a         2997 c         3115 g         3982 t
ORIGIN      
        1 gcctcattta gtaatgcttc aaccttctaa aggagatata catttttgaa caaaggaaaa
       61 acatgcagat ttagtttcct aaaatgattt gacaatatat tttatttagt ttaaaactta
      121 ctgaagtttg tagacttagt cttttaaact aggtcatttt ttttaatttc attaattaag
      181 gaggagtatg agaataaaaa aattagcatt aacagcatta acactatttt tttcatttag
      241 tctttatgct gctgagacaa taaaaattgg agcttcaatg cctttgaccg gaatgtttgc
      301 ggttcctgga tcgaagcacc ttgaaggtta ccagatgtgc gtcgatatca taaatcaaaa
      361 cggcggctgg ctaggaaaag atgtaatact tttggcaata gataatcgtt ctgatactga
      421 aacttccatt tctcaatacg agcgatttat aaatgtagac aaggttgatt tagtatttgg
      481 caccttttca agcaaactga cttttccaac ttccacaatc cttgctaaat acaattatgt
      541 tctacctata ccatcaggtg gagcattgag gatctacagg cgtggtttca aaaatctttt
      601 ttatttccag tcaaatccgg ctgaatatta tggcaagcaa ctgcttgcag tggttcgtga
      661 tcgtgcatcg gcttcagatc agccgaagac ggtggcgttg gtacatgctg acgatttttt
      721 cgccaattcg attgccaatg gtcttctggg caagaaagtt aaattacctg gcagcggcaa
      781 acttgttgaa gacatggccc cgggttacat agtcgaagct ggaatgaaac tagtctatca
      841 tgaacaatgg cccgaagaag gtttttcaga ttggttgact ttagcaaact caataaaaaa
      901 ttcaggtgca gaaatggtta tcggtatgat ggcatccccg gaggaagcgg ttcagctttc
      961 ccgtgcactc aaaaccgtac gccataatcc aaaacacctg atgctgactc aaggtaccca
     1021 gtcagagttt cttgaggggc ttggggatgc aacagaagga atcatgattt acgccgcgtg
     1081 gcatgatgat gctccatatg aagggacact ggccgggcag aggatgaata actctgattt
     1141 tgtagcaaaa tacaaagcca tgaacggcaa gctgcctgac gaagacgtgg ccattacgtt
     1201 tgccctttgc caggggattg accaggcagt caatgcgacg cgttcagtgg acaatgccag
     1261 aatcatgtcc tggttgcata cgaggacttc aagaaatccg gtcaaaacga ttcttggaga
     1321 ttactactgg gattcaactg gattgccgat taataagcag gctctgatag tacaatggca
     1381 gggcagtaaa ctgaaattca tttatccaac caatgaattc caggcttcat caatgatcag
     1441 tcctaaacct gcctggtgat ccaataactg aaatgttttg tttaagggta gttgtgagtt
     1501 tttgctccaa ctaccctttt ttttgagaaa tcagaattgc ctttgaattc agtgattctt
     1561 taaagggata aaaagtcttg gaaaaaaatc cctaaaagac actacttatt ggtatccacg
     1621 caaacaactg tcaagaaagc tacttgattc tgattgaaga ggaaggcacc gaattcaatg
     1681 tcaattcttc ctatgagaac caagacgaga ttgaccgtgt tgtgctggga agtgattcct
     1741 gctggaacaa gtccaaggaa cgccagattg agattgccac caagcacaag gggttgagtg
     1801 gggacgatca agcgctgatt ctcaacacca cttccaagtg cttactaggc atatgatctc
     1861 cgtaggaaaa acgcatgccc aaggttagtc tcaatgttaa tggtaaagtg catcaccttg
     1921 atgttgatga gaagacaccc ttgatttatg tactccgtaa cgatcttggg ctcaaaggaa
     1981 cgaagctcgg ttgcgggctt gagcaatgtg gcgcctgtaa ggtcatagtt gacggcaaag
     2041 cagaattttc ctgcaacacc ccagtggagg cctttgagaa aagttcaatc accaccatcg
     2101 aaggcatagg tacgccagat aaattgcacg tgatccaaca agccttcgtc actgaatgcg
     2161 ctgcccagtg tgggtattgt atacctggta ttatagtggc gataaaggcc ttactggatg
     2221 taaatccaaa tcctgacgat gaaatgatat gtgaggcctt gtctgataat ctctgtcgct
     2281 gcgggaccca cggacaaatc ctcaaagcgg tgaagcgggc agccaaggag ataagcttat
     2341 gaccctttca tcgagtttcg gcaataatcc ggacttggac tcctggctgc aattccacag
     2401 agacggacga atcacggttt ataccggcaa ggttgaattg ggccagaagc tgacaaccgc
     2461 gttgagcgtg attgtggcag aagaacttga tgttgacccc ggacggatca cggttctgac
     2521 agcagatacg gacaaatcac ccaatgaagg atataccgcc ggaagcaatt ccttggaaag
     2581 cagtggcata gcacttcgcc aagcctcggc gaaagcacgt cgggccctta tggagaaggc
     2641 ctcggagcac ctggaagagc ccgtggaaaa cctttctgtg actgacggcg aaatcggggg
     2701 ggccacaacc aataaaaatg tgacatattg ggatctgatc ggagacaagc acttcaaaat
     2761 tacggttact gatgatgtcc tacctaaaag tccagaccaa taccatttga ttggcaaccc
     2821 ggtgccagcc gtcgggctcg aagccttggt cacaggcacg atgacttatg tacacgatat
     2881 ggaatttaca gatatggccc acgccagggt cattcgaccg cccaactacc acgcccaaat
     2941 tcaggatctg aatgacacag aagttcgtgc aatgccagga gtgatcgaag ttatacttga
     3001 tgggagcttc ctgtcggtcg tttgcgaacg tgaagaacag gcaatatggg cagccagtaa
     3061 actggcaaga ataactgttt gggatgaaag caaagagctt aattcgaaag acatttttgt
     3121 tcagctcaaa aacaatccta gacaaagcct gccggtcgtc gagggggtcg caaaagagtc
     3181 tcctgtgcaa ccgtttaggc cgcctgaaaa ttcgtctaaa accctttcag ctacgtatca
     3241 acgaccctac cacatgcatg gctccatcgg cccgtccgca gcaattgccc tatacgatgc
     3301 ccaagtcctc aaagtttgga cacactcgca aggtattttc cccctccgcc tcgcgctggc
     3361 agatgttctg gggttggcag aggacaagat ccatttggta catgttccgg gcgctggttg
     3421 ttacggccac aacggagctg atgacgtcgc cctggatgct gccctggcgg cgcgggctgt
     3481 tccaggacga ccaattatgt tgaaatggga acgtgccgat gaacatgctt gggagccctt
     3541 tggctctgcc atgcaaatag aactacaggc aagcctagat gacacaggcc gagttattta
     3601 ttggaatcac gagacattta gcgacacaca tatgagccgg ccctcagctc atagggaaaa
     3661 ttctcgcctg cttgctgcat ggcatctgca atccccgcgt ccagcaccgc cggtgaagcc
     3721 cacccccggg tttcacactg gcattcatcg taacgctgat cccatttatg actttgccga
     3781 tcgccgtgta gtaaagcatt tggtacatga cttgccattg agagtctctg cgttacgctc
     3841 gttgggtgca tacgccaatg tcttcgctat cgagtctttc atggatgagt tggcccattt
     3901 cagagacatg gaccccttgg aatttcgtct ggcccatctg agcgataatc gcgctcgggc
     3961 tgtgcttgaa gcagcaacct caaaggcaaa ttctggtttc agcagcgctg acgacgggca
     4021 tggcaggggt cttgccgttg cacgttacaa gaattcaaaa tgttatgtag ctgttgtcat
     4081 tgacctcatc gtcgatgact atggtgccat acagcttctg cgggcggtga ttgcagcgga
     4141 tgctggccaa atcgtcgatc ccgatggact gcgcaaccag cttgaaggcg gcttgatgca
     4201 gtctgccagt tggacactca aggaacaggt gaactttgac gacaagggga ttgttagtcg
     4261 ggattgggaa agttatccaa tactaacttt tgcagaggtg ccggaaatcg aaactgtgct
     4321 gatcgaccac caggattgtc catttctagg ctcaggggag gcaactcagg gaccaacggc
     4381 agctgccatt ggcaatgcca tttttgactc cgttggatta aggttgcgtc gtattccatt
     4441 cactcaggac cgagtgcgag atgctgctgc tgaagtttga tggttaacaa cgcagttgtc
     4501 ttcgcccggc tatcgagttc aacgaatgac caaacagctt tacacacatg atcagcaagt
     4561 tctctcttta gcaatctaat aacaatacca ctgagagaac tcttattttc ttcactcata
     4621 tacagatata catttttgaa caaaggaaaa acatgcagat ttagtttcct aaaatgattt
     4681 gacaatatat tttatttagt ttaaaactta ctgaagtttg tagacttagt cttttaaact
     4741 aggtcatttt ttttaatttc attaattaag gaggagtatg agaataaaaa aattagcatt
     4801 aacagcatta acactatttt tttcatttag tctttatgct gctgagacaa taaaaattgg
     4861 agcttcaatg cctttgaccg gaatgtttgc ggttcctgga tcgaagcacc ttgaaggtta
     4921 ccagatgtgc gtcgatatca taaatcaaaa cggcggctgg ctaggaaaag atgtaatact
     4981 tttggcaata gataatcgtt ctgatactga aacttccatt tctcaatacg agcgatttat
     5041 aaatgtagac aaggttgatt tagtatttgg caccttttca agcaaactga cttttccaac
     5101 ttccacaatc cttgctaaat acaattatgt tctacctata ccatcaggtg gagcattgag
     5161 gatctacagg cgtggtttca aaaatctttt ttatttccag tcaaatccgg ctgaatatta
     5221 tggcaagcaa ctgcttgcag tggttcgtga tcgtgcatcg gcttcagatc agccgaagac
     5281 ggtggcgttg gtacatgctg acgatttttt cgccaattcg attgccaatg gtcttctggg
     5341 caagaaagtt aaattacctg gcagcggcaa acttgttgaa gacatggccc cgggttacat
     5401 agtcgaagct ggaatgaaac tagtctatca tgaacaatgg cccgaagaag gtttttcaga
     5461 ttggttgact ttagcaaact caataaaaaa ttcaggtgca gaaatggtta tcggtatgat
     5521 ggcatccccg gaggaagcgg ttcagctttc ccgtgcactc aaaaccgtac gccataatcc
     5581 aaaacacctg atgctgactc aaggtaccca gtcagagttt cttgaggggc ttggggatgc
     5641 aacagaagga atcatgattt acgccgcgtg gcatgatgat gctccatatg aagggacact
     5701 ggccgggcag aggatgaata actctgattt tgtagcaaaa tacaaagcca tgaacggcaa
     5761 gctgcctgac gaagacgtgg ccattacgtt tgccctttgc caggggattg accaggcagt
     5821 caatgcgacg cgttcagtgg acaatgccag aatcatgtcc tggttgcata cgaggacttc
     5881 aagaaatccg gtcaaaacga ttcttggaga ttactactgg gattcaactg gattgccgat
     5941 taataagcag gctctgatag tacaatggca gggcagtaaa ctgaaattca tttatccaac
     6001 caatgaattc caggcttcat caatgatcag tcctaaacct gcctggtgat ccaataactg
     6061 aaatgttttg tttaagggta gttgtgagtt tttgctacaa ctaccctttt tttgtgggat
     6121 cagaattacc tttgaattca gtgggtctat tatatggata aaaacagacc atgccaaaat
     6181 taagaattaa atatatcagt ccagacaaag ttaaagatca aaaaatactt gatgcaataa
     6241 ctgattctgt catcaaggga actccacgtg cagaaagtca ggctattagg gcacgtgtac
     6301 ctcgtgtttt ctggtctttt caaaacacct ggaaagatgt ttttcttgaa ggaaaggtgg
     6361 atcataatct aaaggaactc tgccgcgttt atgtatccaa gtcagttcaa tgtaagtatt
     6421 gtggcaacca aaggagttca aaatctgcat atgatgggtt ggttgaagat gattacaggg
     6481 atctgctcaa tttcgagaaa tctgagaagt atgacgaacc caccaaggca gctctggcac
     6541 tttcgcaggc aattacatgg gacctggaaa cagatgacga attctggaat cgtctgtata
     6601 gatattttag tgaagaagaa attattgaac ttggttattt cgttggattt accatgggtc
     6661 agcaacgatt caataggtta ctcaatcttc atgaacacct cggcaagacc ggctttgaaa
     6721 acatgaactg aaccggtcaa tctgcaattc tcctgcaatc tttttttctt aaagaacaat
     6781 tttttcatgt gaagatcagt caatgctgaa aataaataat gcaacaaaat catttgctgg
     6841 aattgttgca gtagacaatt gcagttttgc gattaagcaa ggcacaatat ccgccctgat
     6901 cggtcctaat ggagcaggta agacaacact gtttaactgc atttcacgca tctatcccat
     6961 tgattccggg cagatttttt acaagaacca cagactggat ttaatgccgg cgcataagat
     7021 cgcacggtta ggcatcggac gatcatttca gattactcga actctggatc aattatctgt
     7081 cacagaaaac cttgctttgc acacagcagg gggaattgga agtcacctct tccacccagg
     7141 tattaccacc gaaaatagac taaaagcctg cgaggttatg gaatttctcg gaatctcaac
     7201 cttagcggac gtggatatgg ggacactttc tttcggacaa aaaaagctcc tggacctcgg
     7261 taccgttcta atggccgacc ctgatttaat actgctcgat gaacctgctg ccggtattaa
     7321 tttgaggttg cttgaagata ttattaataa gttggttaca ttaaaaaatc agggtaaaac
     7381 tatacttctg attgagcata atatggattt ggttatgggg atcagcgatg aagtgatcgt
     7441 gatgtccgca ggtatcatca tcgcgcaagg aaaaccatca gagatacaga aaaatgcaaa
     7501 agtgcttgaa gcttatctgg gaagcaaagc ttagatgaat agtttactca atatcaacaa
     7561 tttgactgca ggttatggtt atggacctga catcgttcgt gatttcagtt tgacgctcaa
     7621 caagagcgaa gtgaagtgta tcattggacc aaacggtgct ggaaaatcaa cgttattaaa
     7681 gtctatcgca ggcatcctga agccccgttc gggggaaata ttttttgatg gggaaaagat
     7741 ctcagaaaaa gaagcttacc agattatgcg acttggaatc tgtttccttc ctcaagaacg
     7801 ggctatattt ccgaatttga cggtcgccga taatttacgc atgtgtgcct attcgataaa
     7861 agataaaaaa gtggctgaag ccagaataaa agaagccatt gaaatgttcc cggtacttgg
     7921 tcaacgccta aaacaaatcg ccggaacgat gtctggcgga caacaacagc aacttgtgta
     7981 tgcacgtgct tttacaatca aacccaagat cattatgata gatgaacctt ctctaggcct
     8041 agcaccgtca ttggttgaac aaacctttaa tcatattact caaatagcat caagtggtat
     8101 tgcagttatt cttgtggaac aaaatgcaat cagaggactt gaaacggcag ggtacggggt
     8161 ggtaatggat ctaggaaagc tagtttttga aaagcctgcc aaggaagtat tacaggatca
     8221 gagtctgcgt gaactctatc tagggaaagc tttgaaaaaa tgatacagtt tcttcaagta
     8281 ttgattttag gactctgttt gggttttgtg tatgcattga tggtttcagg tctgactctg
     8341 atcttcggtg tgatgagaat cgtgaacctt gcccatccaa tactcatcat tagcggatct
     8401 ttcgtatcct attggttgtt tattttgatt ggcctcgatc ctattctatc tatacccatt
     8461 gctggtctag tcgtagccgc cttgggagta gttatctaca aattgatatt tgagagaaat
     8521 gctgcacaac agacttactc ggaaatgacc gtgctgctaa cattcggaac ggcaatggta
     8581 attgatggaa tactgtcttt tgtctttcac aatactcaac gaaccacctc accaagttat
     8641 gccaccgacg caatcttcat tggggatatt tttatccccc tggctcaatt atactctggc
     8701 ctggtaagtt cggtcatcat agttgggctt gcagtatttc tcaaatattc ggattttggc
     8761 attgcgatca gggccacctc acaaaacaga acttccgcag aacttgttgg agttaacgtt
     8821 aaatttgtct gcctaatttc ctttggtatt ggttgcgcat tggcaggagc tgcaggtgcg
     8881 ctggtaagtt ttattttcag ctttttcccc tcaagacact gggaatggat cgccattatg
     8941 atgtcactgg ttgtccttgg cggtatggga agccttaaag gcgcggtgat cggtgccatc
     9001 gtcctttcag tagtttcatc catggtggga gcatttattt ctccagcctg gtctacaatg
     9061 gttttctttc tctctctgtt tctgatactt atcatcagac ccaggggctt gtttggaatc
     9121 caatcagact gaattcatta ggctcggtgt tatgagaatc ctcaaagaga aaggatcttt
     9181 tttgctaaac aataaaaaaa taattccctt tttaatctta atcgttttga ctggtttgcc
     9241 aataattcag ttttttggaa attataatta tctgattcat attgttctat tttcttttct
     9301 ttatataaca atgtcgagta gttggaacat tattggtgga tacggaggat ttatttcact
     9361 tgggcacaat gttttttttg gtataggtgc ttatttttcc ggtttcattt ttgtgcgtta
     9421 cggaatttca ccttttatca cagctttgat tgcaggcttg gttgctgctg tttttggttt
     9481 tgtggtgggt ttaataacac tgcgtgtaaa aggaccgtcc tacatcattt catctatcgc
     9541 actacttatg atatttagga ttttatttga ccgctgggaa ttaattggtg gtacacatgg
     9601 tatgacactc ccacataata cattcccagt ggaatggtca aagatacctc attactacgc
     9661 actattactt ctaatggtgc tttccattta tctatcatgg tatatccgtc attcaaaatt
     9721 tggtttggca ctgcggtcga tttctcatga tgaaataaaa agcgcagtca caggtatcaa
     9781 tgtaaagata atcaaagtaa cagcgtatgc tctttcagcc tattttatag gtgcagcagg
     9841 tgctatctgg ggggattatc ttacatatgt cagaccaaat atatttttta ttatcctgat
     9901 tgcagcaaac atggtgctga tgtgtatcct gggtggaaaa ggaaccgtga caggtccgat
     9961 aatcggtaca atcgtgatga tatttttcaa tgagtttata ctggcctact ttggagcgac
    10021 tgaacttaat atctttttca ctggtttcct gatgattatt acactgatgt atttccctga
    10081 ggggattgtt ggttccctga aaaaacgcaa attgttacca ggttttcttg actggggttg
    10141 attatgaatc aaaaagatcc acgagttgaa gctgccaaaa tgcattggtc tggacgcttt
    10201 gttaccaacg gtgtaccgct atcggttttt caggatgtta ccgagagtgt gaatcactgg
    10261 gatcattggt gcagtgcatg gtctgaaaag gcctcaatct ttgaagcctt gggtcaagaa
    10321 gctatggatc agggatattt gctttcagca gctcagcatc ttacaaccgc tgcagtctgt
    10381 taccattttg gtaagtttct atttgtacag gataaagaac agatgcgaac cgctcacgcc
    10441 aaggctgttg aatgccgtaa tctagcactt ccccaccttg atcccccagg tgaacgagtt
    10501 aaaattccct ttggtgaaca ttgtttctac gggaatctga gaaaacctgt cgattcaata
    10561 aaaccaccag ttattttaat gacattaggc ctcgatagtg ccaaagaaga aatggaaaca
    10621 aacgaaatta tcttccttga gcgaggcatg gcaaccctgg catttgatgg tcccggacag
    10681 ggtgaaagtg aatatgagca gcctatatgt cctgaatacg aacttccggt aaaatcagtg
    10741 ttggactggt tggagaagcg ggatgatgtt gattctgata gtgtcggtat ctggggcgtc
    10801 agcctgggag gatattacgc acccagggct gctgcctttg atgaccgtat caaagcctgt
    10861 atttccctaa cgggaccgtt taacatgaat gaatgttacg aaaaactgcc tggactaaca
    10921 aaaacagctt ttatgcatcg cagcaacagc tctaatgaag aggaagcgag acaagttgct
    10981 gttcgcatgt cattggaggg tgttgcagaa aaaatttcct gtcctcttta tgttgtcgca
    11041 ggaaaactgg atcgggtgat tccacatcag caggcaatta aactagccca tactgcatca
    11101 ggtgaggttg tgcttaatct tgttgaagat ggtgggcatg ttgccaataa tagagcttat
    11161 aaatatagaa ctcaatccgc cgattggatc gcaaccaaac tgggagttta attacagaat
    11221 catagtttca tacggtatcg aaggttagtt ttttcaacaa acagctaatc tttcaataca
    11281 gatgcgaatc acttcgtcag gatcacgttt ccaccagttt tccgctgaaa agatttcaac
    11341 ttcattaagc atttcaaaac tttcactttc catccatttt cttattcggg aaatgtcgat
    11401 gacgccgtcc cccatcattc cacgatccaa caaaaagtgt tttgtctcca tcagccaatc
    11461 gcagaggtga aatgcaagca aacgttcctt acctgctcgt ttgatctgtg atttaagttc
    11521 tggatcccac caggtatgat agacatcaac tgcaattcca attcctgcac ccataccatc
    11581 gcagatatct aatgcatact tgagagtgtt cacgcaagca cgatctgctg catacatagg
    11641 atgcagaggt tcaattgcaa taggcatttt gacggttctg gcgtattcca aagtcttgaa
    11701 taaaccttcc tccatttgct tgcgactccc aatcaagtcc ttggaattgg gttttaatcc
    11761 tccaacgacc atcaccagac attctgcgtg aattgctact gcttcatcga ctgctttttt
    11821 gttgttatct aaaatattct gtgagagttg gttttccaaa gtgaacattc cgcccctgca
    11881 tagaccactg actcgtagtc cacggtcctg gatcattctg ttggcttcgt caagaccaca
    11941 ctcttttaga ttttcccgcc acggcgcaat tccgcctatt ccaaaccttg cacataaatc
    12001 tatggcttgt cttaaattac actgttcctt tacagttgct gtattgagcg acatctgttc
    12061 agctttaatc atgaacccac acctcttgaa gccaacacgt atttgcctct aaaaacagcc
    12121 aactctgggt ccaataatgc accagcctgg tttgccagac gaatcaattc tgcaaggtga
    12181 agtgtggatc gagtgctttc ctgacctcca agcatggtga aatgattctg aaatccattg
    12241 agataagcca gaaaaaccac accggtttta taaaacctgg taggtgattt aaatatgtgt
    12301 cttgagagcg gaaccgttgg ctcaaaaaga tcagtatatg tattatggtc ccccattccc
    12361 agagcagtaa ctgctgcggc tgccaagcct gcaatcgcat cgaaaattcc aagcagtgcg
    12421 tcggaatatc cgaattcatc tccaagaatt aattctggat agttgaaatc atctcctgta
    12481 tacattttta tgtttttggg aagacgtcga cgcatctgta tttctttttc ttttgacaaa
    12541 agtgaaattt tgatgccgtt aattttagaa gagtttattt gcaaaatgga caggcatgtt
    12601 tccatggctt catcatgact tgtagatccc caatatccct taagttcctg atcaaacatt
    12661 tcacccaacc agtggataat tacaggttgt gaaacctgac caagaatcct gttgtatact
    12721 gtagcataat cctcaggccc acgagcacat gcagttaagg cacggcttgc catcaggatg
    12781 atttttcctc caagactttc caccacttcg cattgttctt catacgcttg aataacttcc
    12841 tcaattgtaa ggtttggttt cggaatcaga tggtctgtcc cggccccaca agctatcaac
    12901 cctttgcagg aattggctgc gtcaattgaa cgacggatta gttcttgtga tgcaacccaa
    12961 tcaagaccca ttccacgctg agctgtgtcc attgcctctg caacaccaag tccctttccc
    13021 cagaggtatt ctctgaaggc tatggtctga tcccagtcta tgttggtatc aagccaggga
    13081 tcataatttt tcaagggatc cacaaccaca tgggccgcgg cgtaaaccgt tcgtggaaaa
    13141 acagaaggta ccacactgag ttctcttggt gtactcaatt caagagtttc aagtgtttta
    13201 tccggtttag gtaataaaag gcttggcact tgtcaaactt ctatttcagg tacatccacc
    13261 caacggcgtt ccttccaact ctgaagagca cattcggcca gttgaacccc tttggctcct
    13321 tcataaaggt catatttcca ggggccatcc tctacaacgt gtctcaggaa ttcctcccat
    13381 tgagattgga atccattttt gaattctgta ttgtcaggca caggttccca atggtcgaag
    13441 taattaatgt tctgaggctg gtcaggattc catgtcggct tcggagtgtt cacatgatgt
    13501 tgggtagaac agttgtgcag tcctgcaaca gcagatccca aagtaccatc gacctgaaag
    13561 gtcaccagat cttcacgttt aacccgtacg caccaagaag aattgatttg tgcaatgatt
    13621 cctccttcta gttcaaataa agcataggca gcatcgtctg catccgaaat atacttctgt
    13681 ccggcttcat cccaccgttc cacaatattt acttgccctg tgcaggagac tgatttgacg
    13741 ggtgcaacca cattatccag tacataacgc caatggcaga gcatatcctt gataatccct
    13801 cctccgtcat ttttacggta attccaactg ggacgttgag ctggaatcat ttctccatca
    13861 aatacccagt agccaaattc tccacgtact gagagaatcc gcccaaagaa accagagtct
    13921 cgaagacgtt tgagctttag caggcctggg agatagagtt tgtcatggac cacaccgttt
    13981 ttaactc
//
LOCUS       DTVD01000301            3701 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_9342_length_3701_cov_250.017005, whole genome
            shotgun sequence.
ACCESSION   DTVD01000301 DTVD01000000
VERSION     DTVD01000301.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9342_length_3701_cov_250.017005"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="EYO68_10980"
     CDS             <1..165
                     /locus_tag="EYO68_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIC08366.1"
                     /translation="IAHENSVSSESVIRDADMAEEMATFTRNQIMMEASTSMLAQANQ
                     NSMTVLKLIG"
     gene            complement(232..3516)
                     /locus_tag="EYO68_10985"
     CDS             complement(232..3516)
                     /locus_tag="EYO68_10985"
                     /inference="COORDINATES: protein motif:HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIC08367.1"
                     /translation="MFKAKIIFNIQFTFFLFLILGFSLGTPNYSTAGEEQPVLTYNWI
                     PQFYGKLKGETKLLYTRSFARSMLRFVDIDDDGDDDLFVGKADGRIAFFHNQGSTATP
                     FFKLITEDLVVIHEGVDKQSNAILIQTILDVGNNAAPEFVDIDNDGDFDLLIGSKDGH
                     IFHYENRGNKLSPRFFRKTPIYMGLKFGGNSVPRLADVNGDRTYDLLVGTGSGKIHIF
                     FNSGMTQEAVFCRAFKVSDPPDKRCKFQPELVADIAPQADAVPELVDWDQDKDLDLVV
                     GKSNGKINYYLNTGNNFAPLWSLKSRHFQFIDVGGSAAPTFHDLNQDGFSELFLGTSS
                     SKIIYYENHEILLDLLRKISAIQLVKIDMSASFEKVLSQACSQLRGLPECLVPLAVAF
                     GVPEGTKINRMVELYPFILRPDLSIKSNSDNTADFAEVSSNSIKSEADTSKQQNSVVN
                     ANIQDDNKENPENAVTQADQATAAGESETEKDVIKEKIEKLTSQGIITRNQLWLSSRN
                     FLKIENLIGSDRHSFLTSGDWNQDGRIDILLGSRSGKIFAFENRADKGTDWYELKFPV
                     LENNKRQYSSPVLSDIDADGDLDIISGNRNGKLEWILNRGSEKRPEWVVHDVNLSQID
                     VGSFSTPLLNDMDGDDDLDLLVGNSKGLIIYYENQGNKNTPHFVLRNTRIAGFQMKAS
                     SAPTFWKWNGDKHPDLVVGGREGFLSLISHLPPESSPALGGWTLEAEHWQNIKAIGYS
                     TPHFVDFEGDNKTDLLIGDVQGNLLYWKNGGLKKTIESKQESTLVLTENTLEDEEQEE
                     ENEQADSTIITVENTETEQDSKAIGPIEPSFEFVSSRYGNLELGRRAFPAFMDVDGDN
                     NLDLIVGNSAGELRYYRRELGSGDALWTLESKHFLGYQGRKNSAPVFADLDGDGDFDL
                     LVGNQEGSIDYWENKGNSEIADFVYNPTQFIGVTGGRNSVPAVLDLNGDGRNDLLTGN
                     FIGQLRKFDRIEQGNGFYFRLERRKYLNLDIGIGSVPKITDLNNDQQPDLIIGSDSGN
                     IISFQPDPEKPGILSWKPSPEYFKQLNLPIGGNPEFVDLDTDGDLDLIVGSEEGTLYY
                     FRNTGR"
BASE COUNT         1056 a          798 c          703 g         1144 t
ORIGIN      
        1 attgcacatg aaaattccgt gagttcggaa tcagtaattc gtgatgcgga catggccgaa
       61 gaaatggcaa cgttcacacg taatcagatc atgatggaag caagtacttc catgctggca
      121 caggcgaatc agaattctat gactgtgctg aagttgattg gatagactat tattccagtg
      181 ggcatcaagt actcatttgt gtttgtgctc actgtactcc agttgaaatg atcagcgccc
      241 tgtgttacgg aagtaataca gggttccttc ctcactgcct actatcaaat ccaagtcacc
      301 atctgtatcc aaatcaacaa attcagggtt tccaccaatt ggtaaattca gttgtttaaa
      361 atattccgga gaaggtttcc aggacagaat acctggtttt tccggatcag gctgaaagct
      421 gatgatattt cctgagtcag agccaataat caggtctggc tgctgatcat tattgaggtc
      481 agtaatttta ggtacagaac caataccaat atctaaattt aagtatttgc gtctttcaag
      541 tcgaaaataa aaaccgttgc cctgttctat acgatcaaat ttacgcagtt gaccgatgaa
      601 gtttcctgtc agcaaatcat tacggccgtc accattcagg tctagaacag ctgggactga
      661 gtttcttcct ccagtcacac caataaactg agtaggattg tagacgaagt cagctatctc
      721 agaattgcct ttattctccc agtagtcaat acttccttct tgattgccaa ccagcagatc
      781 aaaatctcca tcaccatcca aatccgcaaa aaccggagcg gaattttttc tgccctgata
      841 gcctagaaag tgcttgcttt caagagtcca cagcgcatcg cctgagccta gttcacgtcg
      901 gtagtatctt aactcaccgg cactgtttcc aacaattaaa tcaagattgt tatcgccgtc
      961 cacatccata aatgcaggaa aagcacgacg cccaagttcc aggttcccat acctggaact
     1021 tacaaactca aaagatggtt caataggtcc gattgcttta gaatcttgtt cagtttcagt
     1081 attctcaact gtaataatcg tgctgtctgc ttgttcattt tcttcctcct gctcttcatc
     1141 ctccaaagtg ttttctgtaa gaacaagcgt actttcctgt ttagactcta ttgtcttttt
     1201 caggccgcca tttttccaat aaagcaaatt accttgtaca tcaccaatca gcaaatctgt
     1261 tttgttgtca ccttcaaaat ccacaaaatg cggtgtgctg tacccgatgg ccttgatatt
     1321 ttgccagtgt tcggcctcca gggtccatcc acccagtgca ggggaacttt caggaggaag
     1381 atgactgatt agacttaaaa atccctctcg cccgccaaca acaaggtcag ggtgcttgtc
     1441 tccgttccat ttccaaaaag ttggtgcaga actggctttc atttgaaacc ctgcaatgcg
     1501 tgtgttgcgc agcacaaaat gcggtgtgtt tttgttacct tgattttcat aataaataat
     1561 caagccctta ctgtttccta ccaggagatc caggtcgtca tctccatcca tatcatttaa
     1621 caatggtgta ctgaaacttc ctacatcaat ttgtgataaa tttacgtcat gtaccaccca
     1681 ttccggtctt ttttctgaac cgcggtttag tatccattcc aattttccgt ttctatttcc
     1741 gctgataata tctaaatctc catctgcgtc aatatctgat aaaacaggag aactgtactg
     1801 tctcttatta ttttcaagta cagggaattt aagttcatac cagtctgtgc ctttatcagc
     1861 acggttttca aaagcaaata ttttccctga acgactgccg aggaggatat ctatccggcc
     1921 atcctggttc cagtcgccgc tcgtaaggaa ggagtggcgg tcactgccaa ttagattctc
     1981 tatcttcaaa aagtttctgc tggataacca gagttgattt cgtgtaataa ttccctgaga
     2041 agttaatttt tcaatttttt cttttatcac atctttttca gtttctgatt ctccagctgc
     2101 agttgcttgg tctgcctgtg ttactgcatt ttccggattt tctttgttgt catcctgaat
     2161 gtttgcatta acaactgagt tttgttgctt agaagtgtct gcttcacttt taattgaatt
     2221 actggaaacc tcagcaaaat cggcggtatt atcggaattg gattttatac ttaaatccgg
     2281 acgcaaaata aaggggtaca actccaccat tctgttaatc tttgttcctt caggtacacc
     2341 gaaagcaact gcaagtggta caaggcattc cggaagtcct cttaattgag aacatgcttg
     2401 agagaggact ttttcaaatg aggcagacat atcaatcttt accagctgaa tagcagatat
     2461 ttttcgaagc aaatccagca ggatttcatg attttcataa tagataatct ttgaggaaga
     2521 cgtacctaaa aatagttctg aaaaaccgtc ctggttcaaa tcatgaaatg taggtgcagc
     2581 actgccgccg acgtcaataa actggaaatg tctggatttt aggctccata atggagcaaa
     2641 gttatttccg gtattgagat aataattgat ttttccgttt gattttccaa caaccagatc
     2701 caaatcctta tcttgatccc aatccaccaa ttcaggaacg gcatcggctt gaggggcaat
     2761 gtctgcaact agttcaggtt gaaatttgca acgtttatct ggtggatctg agaccttaaa
     2821 cgctctacaa aaaacagcct cctgggtcat tccggaatta aaaaatatgt gaattttacc
     2881 tgaacctgtg cctaccagta aatcataagt tctgtcaccg ttaacatcag ctaaacgcgg
     2941 cacactgttc cctccaaatt tcagacccat gtaaatagga gttttacgga aaaatcgggg
     3001 gctgagtttg ttcccccggt tttcgtagtg gaaaatatgg ccatctttgg aaccaatcaa
     3061 aagatcaaag tctccgtcat tgtcgatgtc aacaaactct ggagcagcat tgtttccaac
     3121 atcaagaatg gtttgtatta gtatggcatt gctttgtttg tctacacctt catgaattac
     3181 tactaaatct tctgttatca atttgaagaa cggagttgca gtgctgcctt gattgtgaaa
     3241 aaaagcaatg cgcccatccg cttttccaac aaacaggtca tcatctccat catcgtcaat
     3301 atctacaaaa cgtaacatac tccgtgcaaa gctgcgtgtg taaagtaact ttgtttcacc
     3361 tttcagcttt ccataaaact gtggaatcca gttgtatgtc agaacaggtt gttcttctcc
     3421 ggctgtggaa taatttggtg ttcccaaaga aaatcccagt atcaggaaaa ggaaaaaagt
     3481 aaactgaatg ttaaatatta ttttggcctt gaacatgaat cacaaggatt tgggaaaaaa
     3541 tggtgggaag tgagaataat aggaattatc tgtttatgtt ttttgacttt tctaacaaag
     3601 gactatgaag ctttttgaaa actgcaaagt tgattttgga agatgaatca agtataagta
     3661 aaaagtaaaa ttcatcagcg aaatctatag tctcttactg a
//
LOCUS       DTVD01000302            3696 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_9373_length_3696_cov_269.943422, whole genome
            shotgun sequence.
ACCESSION   DTVD01000302 DTVD01000000
VERSION     DTVD01000302.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_3696_cov_269.943422"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="EYO68_10990"
     CDS             <1..1248
                     /locus_tag="EYO68_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIC08368.1"
                     /translation="LFGASPEHDLLLSGKKVHFGTAGAAVHILDPISRKYRESTSHDL
                     YDIARVCDTLEHIHFFQRSIVCRDLEDVREMDITTCYASVAGTKKHVGTSFSFPENVD
                     EALQMLHLIAGSEKAWRERPFVSMSVCHVIPPLKFAEDASACLEAGVKGGMPVLLLSA
                     GQAGATSPATLARCVVQSVAEVIAGLVYVNAIQEGAPAIFGAWPFVSDLRTGAMSGGS
                     GEQAVLMAACGQMSQFYNLPSGIAAGMTDSKIPDAQSGYEKGYTVALAGHSGANLIYE
                     SAGMHASLLGCCLESYVIDNDMLGAINRTVRGIEVNAETLSLDPIRDVCLDGPGHYLG
                     HEQTLNRMQSDYLYPIVGDRENINNWIEQGSTDSIQRAHLKLKEILKTHFPNNWDEDT
                     DSKIREQFPIRLPRNRMQPLDIS"
     gene            1245..3671
                     /locus_tag="EYO68_10995"
     CDS             1245..3671
                     /locus_tag="EYO68_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC08369.1"
                     /translation="MKTHARVVIIGGGAMGVGLLYHLTKEGWNDVVLVEKGELTSGST
                     WHAAGLIPHFIGSLSMAKIHYYGSELYKKLEAETGQATGWHGCGAVRLAINQDQVDWF
                     HYVKGILDQIGVECHLLGPDEIKKVHPLLNTDGVLLGAHTTGDGHTDPSGVTNAMAIG
                     AKNRGAEIYRNNRVTDINALPSGEWEVVTENGTIVCEHLVNAAGSFCSQVAEMVGLKL
                     PMVNMVHQYLMTESIPEVAKLATELPVVRDPRSSCYYRQEQSGLIIGPYEMQAEAWGL
                     NGIDWGFDNALLPPDLERLEPHLAEAAKSLPVFETAGIKKVVSGPITHTPDGNFLLGP
                     APGLRNHWMCCAASIGITQGPGCGKYLAQWMVHGQTEINVREMDPRRYGDWACGKYTL
                     DKSIDEYEHMYATHYPGEFRSAGRPVRTTPIYEKLKSIGAVFAETFGWERAKWFDQKG
                     EGEQYSFRRNNSFSAVAEECRAVRERVGLIDMTSFAKYEISGNDAEKFLNRICANRIP
                     KIGGVSLVHMLTELGGIEGEATITRLEDNLFYVLSAAVAEIHDYDWFSQHILPEENVN
                     ISNVTDDYGVLVLTGPRSRDVLSKLTDADLSNKEFRWMRGKSIEVSGIPLHALRVSYV
                     GELGWELHHPMDKMETLYDAIMEAGEEFGIANFGTYAMNSLRMEKAYKGWGSELTTEI
                     TPMEAGLERFVDFSKCFLGREATLSRKLLGVNTKLVYVSVETADSDCLGNETVLNPEA
                     PSGENIIGVTTSGAYGHTVSQSMAFAYVQPKFAEPGTKFEIRVLGHNCSATVLKEAAY
                     DPQNLRLRDV"
BASE COUNT          974 a          811 c          962 g          949 t
ORIGIN      
        1 ctttttggag cgtctccgga acacgatttg cttttgagcg gcaaaaaagt tcatttcgga
       61 acagcaggag ctgcggttca tatacttgat ccaatcagtc gtaagtaccg tgaatcaacg
      121 tctcatgatc tttatgatat tgcgagggtc tgtgatacat tggagcatat tcattttttt
      181 caaagatcga tcgtttgtag agacctggaa gatgtgcgtg aaatggatat cactacctgt
      241 tatgcctcgg tagccggaac gaagaaacac gttggtacca gtttttcatt tccggaaaat
      301 gtagatgagg ctttgcaaat gctccatctg attgctggaa gtgagaaagc ctggcgggag
      361 cggccttttg ttagcatgtc cgtttgtcat gtaattccgc ctttaaaatt tgctgaagac
      421 gccagcgcct gcctggaagc aggagtaaag ggtggaatgc ctgttttatt gctctctgca
      481 gggcaagctg gtgcaacatc acccgctaca ttggctcgtt gtgtggtaca gtctgtggcg
      541 gaagtaatag ctggactggt gtatgttaac gcaatccagg aaggcgctcc ggcaattttt
      601 ggtgcctggc cctttgtttc agatttacgc acaggtgcca tgagcggggg tagcggagaa
      661 caagccgtct tgatggcagc ctgtggacaa atgtcacaat tctacaacct gccttccgga
      721 attgccgcgg gaatgactga ttcaaaaatt ccggatgcgc aatccggata tgaaaaagga
      781 tatactgtcg cattggctgg acatagcggt gcaaacctta tttatgaatc tgcaggaatg
      841 catgctagtt tgttaggttg ttgcctggaa agttacgtta ttgataacga tatgcttggc
      901 gcaattaacc gcacagtcag aggaatagag gttaacgcag agactctgtc actcgatcca
      961 atccgtgatg tttgtctgga tggacccggg cattatttag gccatgagca aacattgaac
     1021 agaatgcaat ccgattatct ttatcctatc gttggtgatc gagaaaacat caataactgg
     1081 atcgaacagg gttcgactga ttcaattcag cgtgctcatt taaaacttaa ggaaatcctg
     1141 aaaacacatt ttccaaacaa ttgggacgaa gataccgaca gtaaaatcag ggagcaattt
     1201 cctatacgct tacccaggaa tagaatgcaa cctctggata tttcatgaaa acacacgcaa
     1261 gagtcgtcat catcggcggc ggagcaatgg gcgtgggtct gttgtatcac ttgacaaaag
     1321 agggctggaa cgatgtcgtt ttggtggaaa aaggagaatt gacttccggc tcaacttggc
     1381 atgccgcagg attaattccg cattttatcg gctccttgag tatggcaaaa atccattatt
     1441 acggctcaga actatacaaa aaactggaag ccgaaaccgg acaagccacg ggttggcacg
     1501 gatgcggtgc ggtgcggttg gccatcaatc aagaccaagt cgattggttt cattatgtca
     1561 aaggcattct cgatcaaatc ggcgtggaat gtcatttgct ggggccggat gaaattaaga
     1621 aagtgcatcc tctgctgaac accgacggcg ttttgctggg ggcacacacc accggagacg
     1681 gacacaccga tcccagcggt gtcacaaatg ctatggccat cggcgccaaa aatcgcgggg
     1741 cggaaattta ccgcaataat cgtgtcacgg acatcaatgc tttgccttcc ggagaatggg
     1801 aagtggttac ggaaaacgga acaatcgttt gtgaacattt agtcaatgcg gcaggttcgt
     1861 tttgttcgca agttgcggaa atggtgggac tcaaattgcc gatggtcaac atggttcatc
     1921 aatatttaat gaccgaaagt attccggaag ttgccaagct tgcaaccgaa cttccggtgg
     1981 tgcgcgaccc acgatcttcg tgctattacc ggcaggagca gagcggtttg attatcgggc
     2041 cgtatgaaat gcaagccgaa gcgtggggtt tgaatggcat cgactgggga tttgacaatg
     2101 cgcttttgcc gccggatttg gaacgccttg aaccgcattt ggctgaggcc gcaaagagtc
     2161 ttcctgtttt tgaaactgcc ggaatcaaga aagtagtcag tggtccgatc acgcatactc
     2221 cggacggaaa tttcttactc ggcccggctc cgggattacg aaatcattgg atgtgttgcg
     2281 ccgccagcat cggcatcacg caaggtccgg gttgcggaaa atatctggcg caatggatgg
     2341 ttcacggaca aaccgaaatc aatgtgcgcg aaatggatcc gcgccgttac ggagattggg
     2401 cttgcggaaa atacaccctc gataaatcca ttgacgaata cgaacacatg tatgccacgc
     2461 actatcccgg tgaatttcgt tctgccggtc gtccggtgcg caccactccg atttatgaaa
     2521 aactgaaatc aataggtgct gtttttgcgg aaactttcgg ttgggaacgg gcgaaatggt
     2581 ttgaccaaaa aggcgaaggt gagcaataca gtttccggcg caataattca ttttccgccg
     2641 ttgccgaaga atgtcgggca gtgcgggaaa gagtcggttt gattgatatg accagttttg
     2701 ccaaatatga aatttccggc aatgacgcag aaaaatttct gaatcgtatt tgcgccaatc
     2761 gtattccgaa aatcggcggt gtgtccttag ttcacatgct gacagaattg ggcggcatcg
     2821 aaggcgaagc caccattacg cgtttagaag ataatttatt ttatgtcctc tccgccgctg
     2881 ttgccgaaat tcacgattac gactggtttt cgcaacacat attgcctgaa gaaaatgtaa
     2941 acatcagtaa cgtcaccgat gattacggtg tgttggtgct tacaggcccg cgttcacgag
     3001 atgtcctttc caaactgacg gatgcggatt tgagcaacaa agaatttcgc tggatgcgtg
     3061 ggaagtcaat cgaagtttcc ggaattccac tgcacgccct gcgggtttct tatgtgggcg
     3121 aacttggctg ggaactgcac catcctatgg acaagatgga aacgctttat gacgctatca
     3181 tggaagccgg ggaggaattc ggcatcgcca acttcggcac ctatgcgatg aattcgttgc
     3241 gcatggaaaa agcatacaaa ggttggggca gcgaattgac caccgaaatt acacctatgg
     3301 aagccggatt ggagcgtttc gtcgatttca gcaaatgctt cctcggacgc gaagccacgc
     3361 tttcccgtaa attgctgggt gtgaacacaa aactggttta tgtttctgtg gaaactgccg
     3421 attccgattg tctcggcaac gaaacggttt tgaatccgga agctccttct ggcgaaaata
     3481 taatcggcgt taccaccagc ggagcctacg gacacaccgt cagtcaaagt atggcatttg
     3541 cctatgttca acctaaattt gccgaacctg gaactaaatt tgaaatccgt gttcttggcc
     3601 ataactgctc cgcaactgtt ctcaaagaag cagcttatga tccgcaaaat ttacgcttgc
     3661 gtgacgtttg atttgaggtc cttttttgta aacaat
//
LOCUS       DTVD01000303           13837 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_938_length_13837_cov_176.846104, whole genome
            shotgun sequence.
ACCESSION   DTVD01000303 DTVD01000000
VERSION     DTVD01000303.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_13837_cov_176.846104"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(111..1514)
                     /locus_tag="EYO68_11000"
     CDS             complement(111..1514)
                     /locus_tag="EYO68_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIC08370.1"
                     /translation="MSILDERGQVFGSQVPLLIVGAGACGLTAALAASEQGAEVLILE
                     RDARPSGSTSLSSGFIPAADSRWQREKGIEDSPQLFAADIQAKAKGKANRDLVQLTTK
                     SAAPTLEWLADFHGIEFELLEGFLYPGHSKLRMHAVREHTGTSLLQQLHSASHQNELP
                     LVCKATVSGLFVDEEMKVKGARVSRTDGSTETIGCNAMILACNGYGGNPDLLNRFIPE
                     IADMNYFGHLGSQGEAVYWGELLGAELRDMGAFQGHGSVAYPHGVLITWALMMEGGIQ
                     LNLEGKRFSNEHQGYSEQSVSVFAQPEHLAWNLYDLRLHQLGLNFEDYRNAEKIGAIH
                     SAKNVKELSLTTGLPETALRETLRNVESFQTGTEKDPFGRDFTTKPVLKPPYYTVRVT
                     CAIFHTQGGLAVNDEARVLKKDGTPLPNLFAGGGAAQGVSGPAVWGYLSGNGLLTAFT
                     LGRIAGSCAAQLVLGNQ"
     gene            complement(1511..1744)
                     /locus_tag="EYO68_11005"
     CDS             complement(1511..1744)
                     /locus_tag="EYO68_11005"
                     /inference="COORDINATES: protein motif:HMM:PF00857.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIC08371.1"
                     /translation="MGTSPSEHSDLIFADIVIKGVVASTVRDAHVLDFITAVLSDGFA
                     AFSETGQKTSLANLTTVSTLFTCNKVFKVMNSK"
     gene            complement(1870..2475)
                     /locus_tag="EYO68_11010"
     CDS             complement(1870..2475)
                     /locus_tag="EYO68_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HIC08372.1"
                     /translation="MLNLEPSRTGLVIVDMQNDFLHPEGAYGRNGQNCAAISALPGRL
                     VTVANAIRSAGGWIISTHFTLVPDKEAKPFISSHLKKLRPFLGKGDFAPGSFGQALIE
                     DLQPADLTVEKIAFSAFYQTRLEWILRKAGLETLVFAGIVTNGGVASTVRDAHVRDFQ
                     NIVLSDGCAAFSDEVHDATIKSLSTISQITTCNELNTKLKR"
     gene            complement(2485..4554)
                     /locus_tag="EYO68_11015"
     CDS             complement(2485..4554)
                     /locus_tag="EYO68_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIC08373.1"
                     /translation="MNNNISSYVVGVDVGGTFTDIFFLDETTGDCKVAKVQTTLNNQA
                     EGFLAGILKNVDQVADITTVVHGTTVGTNALHERKGAKTGIITSEGFRDVLEMRRRDR
                     PQTWGLWGSFQPVVPRNLRLEVAERTLADGTVQTTVNREEVKQAAHKLLAAGVESVSI
                     VFINSYANPENEQIALKIVKEIWPNEFVEISANILPEIREFERTSTTTLNAYLQPTIG
                     SYLAKIEAGLKDEGFKGQLLIVQSNGGVMTVDTARNLPIRTALSGPAAGVIASAYIAC
                     EADFPHVITCDMGGTSFDVSLVENCKNTLSAQTNIGFGMTIRTPMIEITTIGAGGGSI
                     AKVDSTNLLQVGPESAGSFPGPVCYGLGNDCPTVTDANVVLGRINADKPIGGKLSRLD
                     VKSAKASIEKQIAAPLGLDVMTAAEAIIKVANSKMAGAIRLVSIERGYDPQKFAAMPF
                     GGGGALHVGALIREVGLRSAIIPRYPGINSALGCVIADMRHDFVQTVNGVLGVLDLKK
                     LEQVMLSLARKGLDLLKNAGVSFKSTKVLFAMDMCYQGQTHTLDVSIPVNFNEYSIAI
                     NVKMILDAFEESYRTIYGQPLEGIPVRILNLRTSVLGLRPKFDLTLLAPADGVTLEDM
                     RTDTRQVWFDGAWLETAIYDRLPLSIGVTVPGPAILEQPDSTIFIEPGLRGKVDCFGN
                     LLITNSN"
     gene            complement(4557..6281)
                     /locus_tag="EYO68_11020"
     CDS             complement(4557..6281)
                     /locus_tag="EYO68_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIC08374.1"
                     /translation="MIDPVTLAVINGRLEQIADEMDATLFRSAFNPIIAEAHDASHGL
                     YHALTGETLIQGKSGLPVFVGAMSFAVKSVIDKAQRDGDLADGDIYIFNDPYDGGTHL
                     SDFKLVKPFFHKNKLFCYLASVGHWHDVGGNVPGNYNPVATECFQEGMLIPPVKLFVK
                     GGLRQDIVDILQANSRLPGSLYGDLNGQINALELGERRMSALLDEYGENTVMEVMSEM
                     HSRAAKMMGEHISALPDGTFSAEDFLDNDGIVDTPLKIALDLTVKADRMILDFSRTSP
                     ACAGPVNIALSTAVASVYVALKHLFTEVPANSGVLSPIEIIIPEGSLLNVKAPKPVGG
                     YTETILRIIDVVFCTMANVAPERVNGCAYGTINALSLAGHRRDGSRWVMFSFFGGGHG
                     GHPQGDGLNHGNAPISTATIPPAEILEAAYPVKFTKWALRPDSGGPGRHRGGLGALYE
                     IELLEEVASVFLFGERGKFSPPGVLGGESGALNKFTYEQDDGVHSPLLSSKMNDIKLK
                     KGQKLLLQTPGGGGYGTPCERDPDAVALDVSLGYVSRENAVRQYAVSINDDGTLNKKE
                     THNLRKVS"
     gene            complement(6274..7203)
                     /locus_tag="EYO68_11025"
     CDS             complement(6274..7203)
                     /locus_tag="EYO68_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIC08375.1"
                     /translation="MNNSPVAPDVSVLKTLLSGAEIIIAPGISDALGSVLVEQAGFQV
                     AYLSGASISYTRFGRPDIGLVSMSEVADTLSAITERTTLPVIIDADTGFGNALNVVRT
                     VRQFESAGASAIQLEDQTLPKRCGHLAGKTLVETDEMVGKIKAALDARRSAETLIIAR
                     TDSIAVEGFESALERGERYVEAGADILFIEAPQSEEQLHSVVERFGGRIPLLANMVEG
                     GKTPQKNASELQKIGYSIVIFPGGMVRALAHTAQTYLASLKKHGSTLPFRNNRNVRCG
                     MLDFDSLNELLETRKLLALGAGYDAGNFKVEND"
     gene            complement(7236..7739)
                     /locus_tag="EYO68_11030"
     CDS             complement(7236..7739)
                     /locus_tag="EYO68_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HIC08376.1"
                     /translation="MTNKISTSRAWIFGDDIDTDQLAPGRFMKDSITELASHCLEDVK
                     QNFSANVEEGDVLVAGKNFGIGSSREQAVQALKHLGVSALVAQSFGGIFFRNAINFGL
                     LAVVCAQAKSIQEGDDISIDADVGIIKNRSQNETYACDKLPPQLLDILKHGGLIPHLE
                     IMMKEQE"
     gene            complement(7748..9049)
                     /locus_tag="EYO68_11035"
     CDS             complement(7748..9049)
                     /locus_tag="EYO68_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIC08377.1"
                     /translation="MAETLAEKIIARAANRTKVCPGEIVTCTVDLAMMHDSGGPRRIK
                     PILERLNARVWDTSKVVLVSDHYAPAVDAESAAILNLTRKWAVENGIDNFYDQQGICH
                     VILPERGHLLPGMFVVGGDSHSPTGGAFGCFMFGVGATDMASILITGKTWVKVPNTIR
                     IEITGTPGVDGGIVDGVSAKDIILHLCSRLGVGGAEYQVVEFVGNIISRLSMSERMTL
                     TNMSAELGAQTGLIAADETTFNYIRQTGKKPEHPVRVDNISALQSDDDAYFLATHKVN
                     VAELVPYVAMPDSPDNAVTIEKVSGVQIQQAYIGACTGAKLEDLQMAARVLKNNTVAK
                     GIRLLIAPASIQTTAAAAADGTLKILTESGAILMPSGCGACAGYGAGILAEGEVCISS
                     TARNFKGRMGHPTSKIYLGSPYTVAASAIVGRIADPRDFLN"
     gene            complement(9056..>9156)
                     /locus_tag="EYO68_11040"
     CDS             complement(9056..>9156)
                     /locus_tag="EYO68_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HIC08378.1"
                     /translation="RAYVIENGCVVLSGDADSLINDPELKKTYLGL"
     assembly_gap    9157..9256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9257..9399)
                     /locus_tag="EYO68_11045"
     CDS             complement(<9257..9399)
                     /locus_tag="EYO68_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIC08379.1"
                     /translation="MLEIRNLHAGYGKMRVLHGIDIEIGTGEIVALLGSNGAGKSTLN
                     NNIS"
     gene            complement(9392..10159)
                     /locus_tag="EYO68_11050"
     CDS             complement(9392..10159)
                     /locus_tag="EYO68_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIC08380.1"
                     /translation="MLEVNAVCKKFGGLNAVSATSLVVPKGKIVSLIGPNGAGKTTLF
                     ALISGFLKPDSGRVYFDGKDITNERPHSICRQGLVRTFQVVQPFANLSVKENIAVGAH
                     TRLAKRADALAKATEVARLVGMEKMLQQSAGSLTIAGRKCLELARALSTEPKLLLLDE
                     VMAGLNATEIQEIVNIINGIRDSGVTIFLIEHVMQAVMSLSDYTYVLNQGVMIADGEP
                     REVTSNPAVIEAYLGHGAAERIAAIRTVSSRKESADA"
     gene            complement(10152..11168)
                     /locus_tag="EYO68_11055"
     CDS             complement(10152..11168)
                     /locus_tag="EYO68_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08381.1"
                     /translation="MSNRVRLIGWCTFLVMLSLIPLFITSGRWIDFLEMTMFVAVLGQ
                     GWNILGGYGGQYSFGNALFFGTGAYIQALLQFKFGLSPWFSLWFALAGTALVGAMVGY
                     LSFRYGLRGSYFALITLAFAEAFHVLSRSLTAITEGGHGVQLTLNQNPDQAISTFQFN
                     FAGDFLKAYGFYYTIFSIMLIAFCAVWLMERSRFGAQLVAVRENEDAAEALGINTFRV
                     KMGAICFSASITAMAGVYYTQKFLFIEPGIAYGPAKSVEALFAPIVGGLGTVIGPLLG
                     SFFIHGVGEITKEMIGHVLGNRPGVDLILFGVILVLVLAFLPRGLAGLVEDLWKRIFR
                     GRNA"
     gene            complement(11178..12065)
                     /locus_tag="EYO68_11060"
     CDS             complement(11178..12065)
                     /locus_tag="EYO68_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIC08382.1"
                     /translation="MSLQSYSWQTVLGSSISGVFLGSIYALVALGLTLIYGVLHIINF
                     AHGSLLMLAMYAVFFLWNILGLDPYLAMFIVVPGGFLFGYALQRTVIGKAGHGKDEIT
                     LLVMLGVSIVMENTALFFFTATDRSVRVPYDLDGFDLGVTYVVYPEIAALLGSLLITL
                     VLWFFMSYTDTGKAIRAVSKERQGARLVGINVEHIFAVSFGIGTACVAAAAALLVPTY
                     AVHPQIGYLFVLIAFTVVVLGGMGSFVGALAGGLIIGVTEQLGRLYLGESLGLITISV
                     IFIIVLLFRPTGLFGSKSS"
     gene            complement(12170..13405)
                     /locus_tag="EYO68_11065"
     CDS             complement(12170..13405)
                     /locus_tag="EYO68_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIC08383.1"
                     /translation="MSKGVNRKTRREFIKTAAAGTVVVAAGNLSFPYIVKAAKSVKIG
                     VVHPVTGWAQYSGSQCRFGALMALEEINSAGGIKSMGGAKLEAVLGDAQSKAEIGAAE
                     VEKMNGAGVSSIVGAYASNICLATTQAAAKHGIPHVVDVGVSDKVVSRGLSNTFRFGP
                     GFGIITRVGVESFIEINRAAGSPTKSVAIVHENTTGFGAGIAGIMTKGLKPEGIEVVD
                     VQSHPTPNKDFRNIVLKLKEVDADVIIPSHYYGEGVLFLRTLQRQKLQPKGIYSVLGG
                     ASSQYRFLSEFPNAAQYVMDCNHWFDPNNSASMAARKKTEAAGKHFNYEVFLAYTAMK
                     FTADAIERAGSSDREKITEAMASSNWSGHFMPYGQTKMVNGQNMGAQPLATQVLGKEI
                     EVIAPSKYATAKALFPRPA"
BASE COUNT         3903 a         3375 c         2815 g         3644 t
ORIGIN      
        1 gcaccaaaga cagtgaaagc atgggttgca ggatgctgtg gtcattcatg ctgagcaatt
       61 ttgaaagcag tatgagtaaa tgaacaatat aaacggggaa tcaatctcgc tcactgattt
      121 ccaagcacga gctgtgcggc gcatgatcct gcgatgcgtc caagggtgaa agcagtgagt
      181 agaccgttcc cagaaagata accccagacg gcaggaccgg acaccccttg tgccgcccct
      241 ccccctgcaa aaaggttcgg caatggcgta ccgtccttct tcagaacccg tgcctcgtca
      301 ttgacagcca gtccgccctg ggtatggaag atggcacaag tgactcgaac cgtatagtaa
      361 ggtggtttca gcacaggttt tgtggtgaag tctcgaccaa agggatcttt ttccgttcct
      421 gtctggaagc tttctacgtt tctcagtgtt tccctcagag ccgtttccgg aagtccggtt
      481 gtcagggaca gttccttcac attttttgca ctgtggatcg ccccaatctt ttcagcattt
      541 cgatagtctt caaaattaag tcccagttga tgtagtcgca ggtcgtaaag gttccaagca
      601 aggtgctctg gctgtgcaaa gactgagacc gattgttcgg agtagccctg atgctcattg
      661 gaaaaacgtt ttccttcaag gttaagttgg atgccaccct ccatcatcag ggcccaggtg
      721 ataaggacac catggggata agccacagat ccatgaccct gaaaagctcc catatcacgt
      781 agttcggcgc caagtaattc gccccagtag acggcttcgc cttgactccc caagtggcca
      841 aaatagttca tgtccgcaat ttcgggaata aaccgattca gcaaatcagg attcccaccg
      901 tatccattgc atgcaaggat catcgcattg caaccaatgg tttccgtgct gccgtcagtt
      961 cgtgaaactc ttgctccctt caccttcatt tcttcatcga caaacagacc tgaaacggtt
     1021 gccttgcaga ccagaggtag ctcattttga tgggatgccg aatgaagttg ctgtagcaga
     1081 gaagtgccgg tgtgttcacg aacagcatgc atacgcagtt tcgaatgtcc cggatagaga
     1141 aaaccctcta acaactcgaa ctcgattcca tggaaatctg ccagccactc gagggttgga
     1201 gcggcagatt tggtggtgag ctgaaccaga tcccgatttg cctttccttt tgcctttgcc
     1261 tgaatgtctg cggcaaagag ctgaggagag tcctcaatgc ctttttcacg ttgccagcgt
     1321 gagtctgcgg caggaatgaa tccggaagag agtgaggtcg atccactggg ccttgcgtca
     1381 cgctccagaa tcaacacctc tgctccctgt tcagaagcag caagggcggc ggtcaagccg
     1441 caggcacctg ctcccacaat cagcaacggg acttgagacc cgaagacttg acctctttca
     1501 tcaagaatgc tcattttgaa ttcataacct taaatacctt attgcaagta aacagggtgg
     1561 aaactgttgt caagttggcc agggaggtct tttgccctgt ttctgaaaag gctgcaaagc
     1621 cgtcgcttag tactgcagtt atgaaatcca ggacgtgagc atccctgact gttgaggcaa
     1681 ctacaccctt gataacaatg tctgcaaata ttaagtctga atgctcgctc ggtgaagtac
     1741 ccactcaagc cggctcatat tgtgttgcag aacattatca aacgcaatca acaaggtaat
     1801 tgttggtatg agtcaagaaa ggaagcaagt attataggcg cctcatttga acggtgaaaa
     1861 atagtgtatc taccttttta attttgtgtt taactcattg caagtggtga tctgtgaaat
     1921 ggtagaaagg gactttatgg tggcgtcatg aacttcgtca ctaaacgcag cgcaaccatc
     1981 cgagagaacg atgttttgga aatcccgtac atgtgcgtct cgtacagttg atgcaacacc
     2041 tccattggtc acgatgcctg cgaaaaccaa agtttccagt ccggcctttc gtaaaatcca
     2101 ctccagacga gtttgataaa aagcagagaa agcaattttt tcgactgtca aatccgcagg
     2161 ttgtaaatct tcaattaagg cctgaccaaa acttcccgga gcaaaatcgc ccttacctaa
     2221 aaatggacgc agttttttta agtgtgatga aataaacggt tttgcttcct tgtctggaac
     2281 taatgtaaaa tgagtcgaga taatccaacc tcctgcggat cttattgcat tagccacagt
     2341 gaccaagcgc ccaggaagtg ctgaaatggc agcgcagttt tgtccgtttc tgccgtatgc
     2401 accttccgga tgcaggaagt cattttgcat gtccacgatc accagtccgg tacgagaagg
     2461 ttctaaattc aacatgattt attcctaatt actattagtt attaacaggt tcccgaaaca
     2521 gtcaaccttt cctctcaggc ccggttcaat gaagattgtg ctatcaggtt gttctaaaat
     2581 tgctgggcca ggaacagtca caccgatgga gagaggtaaa cggtcgtaaa ttgctgtctc
     2641 aagccaagct ccatcaaacc agacctgacg agtatctgta cgcatgtctt ccagtgttac
     2701 accgtcggct ggagcaagca atgtcagatc aaatttgggc ctgaggccca gcacagaagt
     2761 tcttagatta agaattctga caggtatgcc ttctagcggt tgtccataaa tggttctata
     2821 actttcttca aaagcgtcca gaatcatttt tacattgata gcaatggagt actcgttaaa
     2881 attgactggg atactaacat caagggtatg agtctgtccc tgataacaca tatccattgc
     2941 aaataaaact tttgtacttt tgaaggaaac acctgcattt ttcaacaaat ctaacccttt
     3001 tcgtgccagt gataacatga cttgttccag tttctttagg tctaaaacgc ctaaaacacc
     3061 attaaccgtc tgaacaaaat catgtcgcat atcagcaatt acacaaccca gcgcactatt
     3121 gatccccgga taccgtggaa ttatggcact ccgcagtccg acttccctga tcagtgcgcc
     3181 aacatgcaag gccccaccac cgccaaaagg catcgccgca aacttttggg gatcatagcc
     3241 acgttcaatg gaaaccaacc tgattgcacc agccattttt gaattagcaa ccttgataat
     3301 tgcttcggcg gcggtcatga catcaagtcc aagtggtgca gcaatctgtt tttcaatgga
     3361 tgctttagca cttttcacat ccagccgaga tagcttacct ccaatcggtt tgtcagcatt
     3421 gatgcgtccg agaaccacat tggcatcggt gacggttggg cagtcgttgc ctaatccata
     3481 acacaccgga cctggaaaag aacccgccga ttcagggcca acctgtaata aattagtgct
     3541 gtcaacttta gcaatggaac caccacctgc gccaatagta gttatttcaa tcatcggcgt
     3601 acggatcgtc atgccaaagc cgatattggt ctgtgcgctg agtgtatttt tacagttttc
     3661 aactaaagag acatcaaaac tggtgccgcc catgtcacat gtaatcacgt gcggaaaatc
     3721 tgcttcgcaa gcaatgtaag cacttgcaat cactcctgct gcaggacctg ataaagcagt
     3781 gcgaatcggc aaatttctag cggtgtccac agtcataact ccaccgtttg actgcacgat
     3841 caacaattgc cccttgaatc cttcatcctt caaaccggct tctattttcg ccagatagct
     3901 tccgatagtt ggttggaggt aggcattgag ggttgttgtt gatgttctct caaattcacg
     3961 aatctccgga aggatgtttg ctgaaatttc tacaaattca ttcggccaaa tttctttaac
     4021 gattttcagg gcaatctgct cattttccgg attagcatag gagttaataa agacaatact
     4081 gactgattca accccggcag caagtaattt atgggcggcc tgctttactt cttcccgatt
     4141 gacagtcgtt tgaacagtac cgtcagccaa agtgcgctca gcaacttcaa gccgtaaatt
     4201 acgtggtaca acaggttgaa aactccccca caaaccccaa gtttgcgggc ggtctcggcg
     4261 cctcatttca agcacatcac ggaaaccttc gcttgtaatt attcccgttt ttgctccctt
     4321 tcgttcgtgc agtgcgtttg taccaacggt ggtgccatgt accaccgtcg tgatgtctgc
     4381 tacttgatca acattcttta aaattcctgc taagaaccct tccgcttgat tatttaatgt
     4441 cgtttgcact ttagcgactt tacaatcccc tgttgtttcg tccaggaaaa aaatatcagt
     4501 aaaagttcca ccgacatcga ctccaaccac ataactggaa atattgttgt tcatagtcag
     4561 cttacttttc tcaaattatg ggtttccttt ttattcaaag ttccgtcatc attgatggaa
     4621 actgcatatt gccgaactgc attttcacga ctgacataac ccagagatac atctaaagca
     4681 acggcatccg gatcacgttc gcatggtgta ccgtagcctc caccacccgg ggtttgaaga
     4741 agtaattttt gacctttttt gagtttgatg tcattcattt tcgaggacag tagtggacta
     4801 tgtacaccat catcctgttc atatgtgaac ttgttcaaag ccccggattc tccgccaagc
     4861 actccgggag gagaaaattt tccacgctca ccaaacaaaa agacactagc gacttcctcc
     4921 agcaactcaa tttcgtaaag cgctccaagt ccgccgcgat gtctcccggg tcctccacta
     4981 tccggacgca atgcccattt tgtaaactta accggatagg ctgcttcgag gatttcagcc
     5041 ggtggtatcg ttgcagtaga tatcggtgca tttccgtgat tcagtccatc accttgcgga
     5101 tggccaccgt gtcctccgcc aaaaaaacta aacataaccc agcgactacc gtcacgacgg
     5161 tgcccagcca acgacagcgc gttgattgtt ccataggcac aaccattaac tcgttctggt
     5221 gcgacattcg ccatcgtaca aaaaacaaca tcaatgatac ggagtattgt ttcagtataa
     5281 ccaccaactg gtttcggagc tttgacattc agcagcgaac cttccggaat aattatttcg
     5341 atgggtgata aaactccaga attagcaggg acttctgtaa ataaatgctt gagcgcaaca
     5401 tacaccgaag ccactgcagt cgaaagtgca atattcaccg gcccggcgca cgctggtgaa
     5461 gtgcgtgaaa aatcaagaat catgcggtca gctttgacag ttaagtccaa agcaattttt
     5521 agaggcgtat cgactattcc gtcattatcg agaaaatctt ccgcagaaaa agtcccgtcc
     5581 ggaagtgccg aaatatgttc ccccatcatt tttgcggcac gggagtgcat ttcagacatt
     5641 acttccatga cagtattttc tccatactca tctaataaag ctgacatgcg ccgttcacca
     5701 agttcaaggg cgttaatttg accattaaga tcgccatata aactgccggg gagtctggag
     5761 ttagcctgta aaatatcaac aatgtcttgt cttaacccgc ccttaacaaa caatttcacc
     5821 ggaggaatca acataccttc ctgaaagcat tcggtggcca caggattgta atttcctgga
     5881 acattaccac ccacatcgtg ccagtgacca actgaagcaa gataacaaaa caatttattt
     5941 ttatgaaaga acggcttgac taacttaaaa tcagagaggt gagttcctcc gtcataagga
     6001 tcattgaaaa tgtaaatgtc accatctgct aaatcaccgt cgcgctgtgc cttgtcaatc
     6061 acggacttga ccgcaaacga catggctccg acaaagacag gtaaaccgga tttcccttga
     6121 atcaaggttt caccagtcaa agcatgataa agaccatgag atgcatcatg ggcttctgcg
     6181 ataattggat tgaaggcgct gcggaacaat gtcgcatcca tttcatcggc gatctgttcc
     6241 aatcttccgt tgatgaccgc gagtgttaca ggatcaatca ttttctacct taaaatttcc
     6301 agcatcataa ccggcaccaa gtgccagtaa tttgcgggtt tctaacaact cgtttaagga
     6361 atcaaaatcc agcattccgc accggacgtt ccggttattg cgaaaaggaa gggtcgagcc
     6421 gtgtttcttg agactggcca gataagtttg agccgtgtgt gctaaagccc gtaccattcc
     6481 gccgggaaat ataacaatgg aataacctat tttctgtaat tcagatgcat ttttttgtgg
     6541 tgtcttaccg ccttcaacca tatttgctaa taaggggatg cgtcccccga aacgctcaac
     6601 aacagagtgc aactgttctt cggattgtgg tgcttctata aacaggatgt cagcaccggc
     6661 ttcaacatat ctttcccctc gttccagtgc actttcaaaa ccttcgacgg cgatgctgtc
     6721 tgtgcgggcg attatcagag tttccgcact gcgtctggca tcaagtgcgg ctttgatttt
     6781 acccaccatt tcatcagttt ccaccaaagt tttacctgcc agatggccac atcgtttggg
     6841 cagagtctga tcctccaatt gtatggcaga ggcaccagcg gattcaaact ggcggacagt
     6901 ccggacaaca ttcagagcat tgccgaaacc ggtatcggca tcaataataa ctggcagagt
     6961 tgttctttcg gtaatggcag ataaggtgtc agccacctca ctcatactga ctaaaccaat
     7021 gtccgggcgg ccaaatcggg tataggaaat agacgctccc gaaagataag ccacctgaaa
     7081 accagcctgt tccacaagga ctgagccaag ggcatcactg attcccggcg caattataat
     7141 ttctgctcct gataacaaag tttttaaaac agaaacgtca ggtgcaactg gagaattatt
     7201 catgtttttg tttatatttg cactggattt tctagttatt cttgttcttt catcatgatt
     7261 tcaagatgtg gaattagccc tccatgtttg agaatatcaa gtaattgagg tggcaatttg
     7321 tcacaagcat aggtttcgtt ttgagacctg tttttgatga taccaacatc agcatcgata
     7381 gaaatatcat caccctcttg aatacttttt gcttgtgcac aaacgactgc aagtaaacca
     7441 aaatttatag catttctaaa aaagatccct ccaaaagatt gggccactaa agcactcacc
     7501 cccagatgtt tgagcgcctg cacggcttgt tcccgtgaag agccgattcc aaaatttttt
     7561 ccggcaacta acacatcacc ttcctctaca ttggcagaaa aattttgctt aacatcttcc
     7621 agacagtgac ttgctaattc agtaattgag tccttcatga aacgacccgg agccaattga
     7681 tcagtatcaa tatcatcacc aaaaatccaa gctctacttg tagaaatttt gttcgtcatt
     7741 tttggtttca gttcagaaaa tcacgggggt ctgctatgcg tcccactatc gcacttgcag
     7801 caacggtata aggagaacca agataaatct ttgaagttgg atgccccatt cgtcctttaa
     7861 agttccgggc agttgaggaa atacagactt ccccttctgc caggattccg gcaccatagc
     7921 ctgcacaagc accacatcct gagggcatca agattgctcc ggattctgtc aaaatcttca
     7981 atgtgccgtc tgcagccgcg gcggcagtgg tttgtattga agcgggagca atcagcaggc
     8041 gaatcccttt agcaacggta ttatttttca gtacacgggc tgccatttgc aaatcttcca
     8101 acttagcacc tgtacaagct cctatgtagg cctgttgaat ttgaactcca gaaacttttt
     8161 caattgtcac agcgttgtca ggactgtctg gcattgccac atatggaaca agttccgcaa
     8221 cattcacttt gtgggtcgcc aaaaagtaag cgtcgtcatc gctttgcagt gctgaaatgt
     8281 tgtcaactcg caccggatgt tcaggctttt ttccagtttg ccgaatataa ttgaaagttg
     8341 tctcgtcagc ggcaatcaag ccggtttgtg cccctagttc tgcggacatg tttgtcaatg
     8401 tcatgcgttc agacattgac aggcgcgaaa ttatgttacc cacaaattca acaacttgat
     8461 attctgcccc acccacgccg agtcgtgaac acaggtgtaa aataatgtcc ttagctgaaa
     8521 ctccatctac aatcccgcca tcaacaccgg gtgttccggt aatttctatc cgaattgtat
     8581 ttggcacctt aacccatgtt ttgccggtaa ttaaaatgct ggccatatcg gtcgctccaa
     8641 caccgaacat gaaacagcca aaagcccccc cggtaggtga atgactgtct ccgccaacaa
     8701 caaacatacc cggcagaaga tgcccccgtt caggtaatat cacatggcaa attccctgct
     8761 gatcataaaa attatcaatc ccgttttcaa ccgcccactt gcgggtcaag tttaaaatgg
     8821 cggcactttc tgcatctact gccggagcat agtgatccga aactaagacc actttactgg
     8881 tgtcccagac cctggcattg agacgctcca ggatcggttt tattcgacga ggtcctcctg
     8941 aatcgtgcat catggcgaga tcaacagtgc aggtaacgat ctcaccagga caaaccttcg
     9001 tccggtttgc ggcgcgtgcg atgatttttt ccgcaagtgt ttcagccatt gccactcaaa
     9061 gccccaaata agtttttttc aattccggat catttatcaa actgtcagca tcaccggaca
     9121 acacgacaca accgttttca atgacgtagg ctctggnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnngaga tattattatt taatgttgat tttcctgcac cgttactccc
     9301 caatagagcc acaatttcgc cagtcccaat ttcaatatca ataccgtgca acactcgcat
     9361 ttttccatat ccagcatgga gattacggat ttcaagcatc agctgattct ttcctggaac
     9421 ttacagtacg gatagccgca attctttctg ccgcaccatg acccaaatag gcttcaatta
     9481 cagcaggatt gctcgtaact tctcgtggtt caccatcagc aatcatgacg ccttgattga
     9541 gaacataggt ataatcagaa aggctcatta ctgcttgcat tacatgttca atcaaaaaaa
     9601 tggtcactcc tgaatctcta attccattaa taatattaac tatttcttgg atctctgttg
     9661 catttaaacc ggccattacc tcatccagca gcaaaagttt aggttctgta gacagagcac
     9721 gagcaagttc caaacacttg cgaccggcta tggtcaaaga tccagctgat tgctgaagca
     9781 ttttttccat accgacaagc cttgcaactt cagttgcttt agccagcgca tctgcacgtt
     9841 tggctagccg tgtatgtgca ccgacagcaa tgttttcctt tacgcttaaa tttgcaaaag
     9901 gttgaacgac ttgaaatgtc ctgaccagcc cttgccggca aatactgtgt ggacgttcat
     9961 ttgtaatatc ctttccatca aagtaaaccc taccgctatc aggttttaaa aaacctgaaa
    10021 tcaaagcaaa taaagttgtt tttcctgcac cgttagggcc gattagtgaa acaattttgc
    10081 ctttcgggac aacaagactt gttgcagaaa cagcattcaa accaccgaat tttttgcaaa
    10141 cagcattaac ttcaagcatt acgccctctg aaaattcgct tccatagatc ttcgaccaaa
    10201 ccagctaaac ctcgtggtaa aaatgcgagt acaagaacta ggatcactcc gaataaaatt
    10261 agatcaacac ctggacggtt acccaaaaca tggccaatca tttcctttgt tatttcacca
    10321 actccgtgaa tgaagaaaga accaagcaaa ggaccaataa ccgtaccaag tccaccgacg
    10381 attggtgcaa acagagcttc aaccgatttg gcaggaccat aagctatccc gggttcgatg
    10441 aaaagaaatt tctgagtgta ataaactccg gccattgctg tgattgatgc cgaaaagcaa
    10501 attgctccca tctttactcg gaaggtattg atacctaaag cctccgctgc atcctcgttt
    10561 tcgcgaacag caaccaactg tgcaccgaaa cgcgaacgct ccatcagcca gactgcacaa
    10621 aatgcaatga gcatgataga aaatatggtg tagtaaaaac cgtatgcttt gagaaagtct
    10681 cccgcaaaat taaactggaa agtagaaatc gcctgatctg gattttgatt gagtgtcaat
    10741 tgtacaccat gaccaccttc ggtaatagca gtcaatgaac gtgataagac gtgaaaagcc
    10801 tccgcgaatg ccaaggttat caaggcaaag tatgaaccac ggagtccata acgaaagctt
    10861 aggtaaccta ccatggcccc aaccagagct gtaccggcca gggcaaacca caaggaaaac
    10921 caggggctca aaccaaattt gaactgtaat aaggcttgaa tgtaggctcc tgtaccaaaa
    10981 aataaagcat tgccaaatga gtattgtcca ccatatccac caagaatgtt ccagccttga
    11041 ccgagtaccg caacaaacat ggtcatttca agaaagtcaa tccagcgacc agaagtgatg
    11101 aacaaaggaa taagggacaa catcactaaa aacgtacacc aaccaatgag acgaacacga
    11161 ttactcattt ccctctccta actcgattta ctaccgaaca atcccgtcgg acgaaataag
    11221 aggacgatta taaaaatcac ggaaattgtt atcaatccaa gactctcacc caaataaagc
    11281 cgccctaact gttctgtaac tccgattatc aaaccaccgg ctaaggcccc tacaaaacta
    11341 cccatgcctc ccagcacgac caccgtaaaa gcaatcagca caaacagata gccgatttgt
    11401 ggatgtaccg cataagttgg caccagcaga gcggcagcag ccgcaacaca ggctgtaccg
    11461 atcccgaaac tgacagcaaa tatatgttcc acattaattc caaccagacg tgctccttgt
    11521 cgctcctttg aaaccgctct gattgcttta ccggtgtcgg tatatgacat gaaaaaccat
    11581 agaactaaag tgatcaggag agagccaagg agcgctgcta tttcaggata aacgacgtaa
    11641 gtgacaccaa gatcgaaacc atcgaggtcg tagggaactc tgacagaacg gtcagtagct
    11701 gtgaagaaga acaaggcagt gttttccata acaattgaaa cacccagcat gaccagcagg
    11761 gttatttcat cttttccgtg accggccttg ccgattactg tgcgctgtag agcataacca
    11821 aacagaaacc cacccggaac tacgatgaac atagccaggt aaggatctag acctaaaata
    11881 ttccaaagaa aaaaaactgc atacatggcc agcatgagca ggcttccatg tgcgaaatta
    11941 atgatgtgca acacaccgta gatcagtgtt aaacctaagg ccactaatgc gtaaattgaa
    12001 cccaggaaaa caccgctaat agaagaacct aaaacggtct gccagctata tgactggagg
    12061 gagataccta gaaagtcaat tgtcacagct atgcttattg tttattaagg aatggtataa
    12121 aaacgactga gggaatgtac cccagtcgat tttatgtcgc ataaaaggct tatgccggtc
    12181 gcgggaagag tgcttttgca gtagcatatt ttgatggggc tatcacctca atttccttac
    12241 ccagtacttg agtggcaaga ggttgggctc ccatattttg tccatttacc atttttgttt
    12301 ggccgtatgg cataaaatgt ccggaccaat tactcgaagc cattgcctca gttatcttct
    12361 cccgatcact ggaaccggca cgttcgattg catcagctgt gaatttcatt gctgtatagg
    12421 ctagaaagac ctcataattg aagtgtttac cagctgcttc tgtcttcttg cgtgcagcca
    12481 ttgaagctga attattagga tcaaaccaat ggttacagtc cattacatat tgtgctgcat
    12541 ttggaaactc actcaggaag cgatactgcg acgaagcccc accgagaacc gagtaaatac
    12601 ctttaggctg cagcttttga cgttgaagag tgcgtagaaa caaaacacct tctccataat
    12661 aatgagatgg aataatcaca tctgcgtcaa cttctttgag tttaagaaca atattgcgga
    12721 agtctttatt tggagtggga tgtgactgta catcaacaac ttcaatgcct tccggcttca
    12781 ggcctttagt cattattccg gcgatacctg caccaaaacc ggtagtattc tcatgcacaa
    12841 tggcgacact ttttgttgga ctccctgccg ccctgtttat ctcgataaaa ctttcgactc
    12901 caacccgagt aataatacca aatcctggac caaatcgaaa cgtattgctt agtcctcgtg
    12961 agacaacttt atcactaaca ccaacatcaa caacatgtgg gataccatgt tttgctgcag
    13021 cttgtgttgt agcaaggcag atattagaag cataagcacc gacaatggaa gagactccag
    13081 cgccgttcat tttttctact tctgcagctc caatctcggc ttttgattgg gcatcaccca
    13141 agactgcttc cagcttagca cctcccatgg acttgatacc tccggcagaa ttaatttctt
    13201 caagtgccat caaagcgcca aagcggcact ggctacctga gtactgtgcc cagcctgtta
    13261 caggatgcac gaccccgatc ttcacagatt tggctgcttt cacaatgtat ggaaaactca
    13321 aatttcctgc tgcaactaca accgttcctg cagctgcagt tttgatgaat tcgcgacgtg
    13381 tttttctgtt aactcctttt gacattttaa tcctcctcat tagaaattat ttttatttaa
    13441 caagtctcaa actgtagaat agcctgagta ctttcatcac taaaccttaa tactgcaatc
    13501 tcatgcagcc aaataggtca gccattacat gtttccaata ttttgttctt atttcatcga
    13561 agtatatttc aatacccaac aagatgatat ttaatcaatt gtactagtac aaacaacaca
    13621 aatagacact atcacaagtt ttggaaacag ctaaaaatac tatttaaaaa gatggtagtc
    13681 aagcagaatt atttatttat ttaattacta ctaaaaaata tttacattct tcagaatttt
    13741 catcaaaagc ataaatagcg attctcttgt tggaatataa tcaatctatg aatgtttcat
    13801 attaagtcca tgtgaatccc cccactaatt cttcaca
//
LOCUS       DTVD01000304           13797 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_943_length_13797_cov_423.265172, whole genome
            shotgun sequence.
ACCESSION   DTVD01000304 DTVD01000000
VERSION     DTVD01000304.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_943_length_13797_cov_423.265172"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            3..404
                     /locus_tag="EYO68_11070"
     CDS             3..404
                     /locus_tag="EYO68_11070"
                     /inference="COORDINATES: protein motif:HMM:PF04982.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08384.1"
                     /translation="MAAGASVGLAIGAMYYTRCIHPPGGATALAAVIGGQNIHALGYQ
                     YILTPIAINTATILLVALLFNALFHWRRYPAFLLSKETEKDYTNSEYAPVDHADFVYA
                     LSQMDTFIDVTEDDLLKIYQLATGREINSNN"
     gene            412..3918
                     /locus_tag="EYO68_11075"
     CDS             412..3918
                     /locus_tag="EYO68_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIC08385.1"
                     /translation="MNSPQPVKKTIKLKNSVRIVTATSLFDGHDAAINIMRRIMQSQG
                     AEVIHLGHDRSVEEIVNVAIQEDAQSIAVSSYQGGHMEFFRYMLDLLREKGSGHIQVF
                     AGGGGVIIQSEIQELTDYGISRVYSPEDGRELGLTGMIADMLKRSDFQVLPDKFEPHA
                     DKLVTENVQHIAHSLTWIEQNVKGSQLAPENKVVEPQEAVQKVLNKLHSQDSPVENSQ
                     APVIGLTGTGGAGKSCLADELLRSFLEEFPEKRIAILSVDPSKRKTGGALLGDRMRMN
                     AINDPRVYMRSLATRRSHLATSTALEGAVSLLQATGKSGGGGFDLILVETAGIGQSDS
                     EISDFVDLSVYVMTPEFGAATQLEKIDMIDFADCIVINKFDKPGAQDALLAVGKQYKR
                     SRNDFDATTETMPVFGVVANRFNDSGTNWFYHKLIEILIKKNSLNWKRSHEAEFAVSE
                     ITELIPSDRKEYLRQIAGCVRSYKKEVRKNAQTAAECGQLHGTLKQMLGETENKNSWN
                     ILQPCPIENIPETAHTVAELYNEKTSKLPESLRLQLSEFHEKNQAYLGENFSFDIRNK
                     ELKISNHNISLSGLKIPKIATPKFNDWGEIASWLGLENFPGSFPFTGGVFPFKREGED
                     PTRMFAGEGIAERTNRRFHLLACDQPATRLSTAFDSVTLYGSNPHLRPDIFGKIGNAG
                     VSICTVDDAKRLYSGFDLLLPKTSVSMTINGPAPVVLAFFMNAAIDQQIEKHLRENGH
                     LEKAQKTLQERYKKQGVEPPEYRMERPENHDGLGLELLGLSGKHFVDNETYGAIKADV
                     LNNVRGTVQADILKEDQAQNTCIFSTNFALRMMGDIQEYFTANDVRNFYSVSISGYHI
                     AEAGANPISQVAFTLANGFTLIEYYLARGLAIDDFARNLSFFFSNGMDPEYAVIGRVA
                     RRIFAVTLRNLYGANERSQKLKYHIQTSGRSLHAMEMDFNDIRTTLQALYAVYDNCNS
                     LHTNAYDEAVTTPTGESVRRALAIQLIINRELGQAFNQNPMQGSFLIEELTELVEEAI
                     LSEFVRLSERGGVLGAMETMYQRNKIQEESLYYETLKHSGELPIMGVNTFLNPNPAET
                     DAEIELARSTEEEKTMQIHDLENFHKFHSKERDSMMERLREAALKNGNIFEVLMESVQ
                     VCSLGQITQQLYQIGGQYRRNM"
     gene            complement(4230..4865)
                     /locus_tag="EYO68_11080"
     CDS             complement(4230..4865)
                     /locus_tag="EYO68_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HIC08386.1"
                     /translation="MENIARLVNFPFSKTEEILVKTALLHNKVLAFPTETFYGLGGNA
                     FFKEVADRVYNIKERPRNKPLLVLTTPEWLPGLCLWNDSRIDDLMNAFWPGPLTLILA
                     ANPELPQHLQNSDGTLAVRYTSAPAAQCLIELGSCPIIGTSANLTGMPECSSATEVHN
                     QLNNKVDVIIDGGELEENRASTILNCQNKNFEVLRHGVITSSEINRICEVS"
     gene            complement(5027..6169)
                     /locus_tag="EYO68_11085"
     CDS             complement(5027..6169)
                     /locus_tag="EYO68_11085"
                     /inference="COORDINATES: protein motif:HMM:PF13458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08387.1"
                     /translation="MKLFRLTVILVFLFSIAHFCFAETGVFPKNIVFVTHQPLSGPAK
                     EYSDIGKSAQAYFKYVNDQGGVHGRSIELKIIDDQLKPYKAKEELEELTLKNDIFAVF
                     SGIGSKTHQAVYPLLKQQRLPSFFVGSDLPEWTQPIRANVFGFMPTAETEARVIGKYI
                     TEKHTGKEFIIWYPEKPVYQRAVKALTKELYSVSAKLLPGKAGRLSAEWQLIRRNNPD
                     FLVVLGNYTDVMNFLKFSPQINIPMLTGHALADSRLPEWLGQKVSERVRVLTAYPLKI
                     ETEHPGQILHKLILSEYAPNLALNRWTLYGHAVAELMVEVLNRTGRSLSRQKAVSAAE
                     SITKWQGKLLPPVYLDSRNHLALTTFRVSRITYGRVSHLSGWIDGK"
     gene            complement(6527..8734)
                     /locus_tag="EYO68_11090"
     CDS             complement(6527..8734)
                     /locus_tag="EYO68_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HIC08388.1"
                     /translation="MAEDFVSLVGTLEKILYTNPENGFLIGTFLTENSIRPITVKGIV
                     FNTHEHETLRLKGSWENHKIYGRQFSIREFMPVEPTSEEGMVRYLSSEVFKGIGKKTA
                     ERIVKKFGNDTFKIIDSSPKLLSKVKGVGRKQQKSLLEAWDDQRGLRDVMTFLRGVGI
                     SHAYAQRIFTKNGLNSIPLIKANPYQLTDIPGIGFLTADGIARNLGFDNNSPHRADAG
                     LLYMLEQQALNGHTCFPRRDLLEKTVQELNIDSSMLESSIRQLLEDRLLNSEKIEDAS
                     GIEQELVYRPRFYKAERRVAENIFRILNSEAYTVYEGESYLIEEQERKVGLKLDPAQR
                     EAVEAALQHKVLIITGGPGTGKTTIVRFILGLMRTRIPAIALAAPTGRAAKRITETTG
                     AAASTIHRLLEASNIGFQRDRENPLEQELLILDETSMIDTLLMDSLLEAVPSASRLIL
                     VGDVDQLPSVGAGAVLSDLIESRSIPVVRLDHIFRQAADSFITVNAHKVRRGEMPDFS
                     SSNRQTEDDDQLLDFYFIKESNPEKIVEKILLMSTERIPQRFELDPMMDIQVLTPMHR
                     GVTGAINLNRKLQDVINPDAKGLEHREQWFRIGDKVMQQQNDYEKLVFNGDLGRIVNC
                     DPKTKELHVKFDQQIVHYQGKEMDQLSLAYAITVHKSQGSEYSAVIVPLTTHHYMMLQ
                     RNLLYTAITRGKQLVVLIGTDAAIKKAVENEGSTRRFTGLLHQLSELGAAPLF"
     gene            complement(8956..9468)
                     /locus_tag="EYO68_11095"
     CDS             complement(8956..9468)
                     /locus_tag="EYO68_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08389.1"
                     /translation="MNYKSGNLNKLIFSKNKLFRRLFLFCVLSFFIAVPGAIARQPTP
                     FELEAIRKETRVVFLNFLQLWQEERYFELYKYGKKHSTDQISIEEFATRMVELDWVPV
                     GLKTGKEPEISYRYLTLLYVNAAIVFRHKSIFDLQFTKQQSFLLLKESGKWRFDLLQM
                     IRSPFYIPPN"
     gene            complement(9465..10541)
                     /gene="holB"
                     /locus_tag="EYO68_11100"
     CDS             complement(9465..10541)
                     /gene="holB"
                     /locus_tag="EYO68_11100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIC08390.1"
                     /translation="MESPFISVLLLMSAFLHFSVPDSTMPFREIYGQEHAIDLMNQAV
                     QRDRMPHAWLFTGQANIGKYKTAVALAQKLNCRKCGEDACGECDICFQIAEQNFLDFQ
                     VLIPDGKFIKIDQIRKSLNWLHLHPDQAKKRVLILDGAQHLGREAANAFLKTLEEPAP
                     KTLLILIADSSQQLLETIVSRCQQIRFRPLSEEISERILRETTNLSTARIQLLSAFSM
                     GSVNGNLAERLELMETVQQTAIRWLTTFSAATLEEMLRTCETWAKSKNEEWRLLLDFL
                     ETWFRDLTWLFRGLPEEKLINRTVGSDENRISALRKSGEHFTLQQIFEIFGWIAESRR
                     TIELNANKSLAVESLCLHMYRRAV"
     gene            complement(10477..11853)
                     /gene="mgtE"
                     /locus_tag="EYO68_11105"
     CDS             complement(10477..11853)
                     /gene="mgtE"
                     /locus_tag="EYO68_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIC08391.1"
                     /translation="MTKSRDTQIHKLVNKLINTERTQLLKSILEPLKPVEIANILLQL
                     KLKQQLVLLENLDRETSSEVLNNLQDSPSILGDIVEQISTDRLSNAIEDMPQDDAADF
                     VGLLDDEKAEALLEKLPEKDREELTNLLQYDEESAGGLMTPYVVAIRRDQTVATAIKE
                     IQKFVKKEGFELFYTAYVVDDHDHLIGTIGTTELLLAERHKLIENLMNPEVVAVDQDL
                     DQEEVARIAQEYDLVVVPVIDKHLRLIGRITLDDLVDVIVDEYNEDMGHIAGTGDEEV
                     SETSVLRASGDRLPWLLVGLFGGFLTAIVMSLYENALISLPDVTYFIPLVAALGGSIG
                     IQSSSIVVRGLATGAIQTTDILVRLWKELRVAFLNGIICSFILAGMSWYLTDDVGRAF
                     TSGFSLLIVVCFAAAVGSTIPIILKRMNIDPAIATGPFITTTSDIIGIAIYLGFAFNV
                     SFSPLFSS"
     gene            complement(11889..13394)
                     /locus_tag="EYO68_11110"
     CDS             complement(11889..13394)
                     /locus_tag="EYO68_11110"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIC08392.1"
                     /translation="MELKIEYSNDSLTSEKLEALVVFVVEDSDLSGSGLRSLPGDLKK
                     NLNASIKLKVFTGKKDKSQHLFSGYAKIPQILAVGLGKKDELDAETLRRAAGKAGKML
                     TLLKANKVGFVVTSLIQNAVEVDAGQVLVEGLTLGAYEFNTYKKSQNEKQNSRSITIK
                     CCDPEKLTSLKVLKKGKIRADGTNLARTLGNTPANDLKPKDLAAEAKRIAVKYKMECT
                     VLEEKDMKKLGMEMLLGVSRGSREPAKLIILEYAHQQAKQTVAIVGKGVTFDSGGISL
                     KPGKNMDEMKFDMCGAAAVLGAMKVIGQVNPKLNIIAVIPTTENMPGGNAQRPGDIVT
                     AHNGKRVEILNTDAEGRLILGDALSYVVKEYKPDAVIDLATLTGACVAALGHLTTGAV
                     SNNDALMEQVRRAGDISGDRVWQFPSFPEYGESIKGKYADLQNIGGGDSGVIVGGLFL
                     EHFVDKTPWVHLDIAGTSWNVKHLGYQPNSGATGVGVRLLVDFVQEWQPLK"
BASE COUNT         3986 a         3044 c         2741 g         4026 t
ORIGIN      
        1 gcattgcagc aggtgccagt gttgggttgg cgattggagc tatgtattac acccgttgca
       61 ttcatcctcc tggcggtgca acagctctgg ctgcagtaat tggcggacaa aacatacatg
      121 ctctgggcta ccagtatatc cttactccaa ttgcaatcaa tactgcaacc attctcctgg
      181 tggcactttt atttaatgcc ttgtttcatt ggcggcgcta tcctgcattt ttgctatcaa
      241 aagaaaccga aaaagactac actaatagcg aatatgcacc agtggatcat gctgattttg
      301 tttatgcact cagccaaatg gacactttca ttgatgttac cgaagatgat ttgttgaaaa
      361 tttatcaatt ggcaactgga agagaaataa actcaaataa ttaaaataac gatgaactcg
      421 ccccaacctg tcaaaaaaac aatcaaacta aaaaacagtg ttcgtattgt taccgccacg
      481 agtttatttg acggacatga tgctgcgatt aacattatgc gccgcataat gcagtcacag
      541 ggtgcggaag tgattcacct cggccacgat cgcagcgttg aggaaatagt caatgttgcc
      601 attcaggaag acgcccaaag tattgcagtc agttcgtacc aaggcggaca catggaattt
      661 ttccgctaca tgctggactt gttgcgggag aaaggttcag gacatattca ggtttttgcc
      721 ggcggcggag gtgtgatcat tcaaagtgaa attcaggaat tgacggatta cggtatcagt
      781 cgcgtttatt ctcctgaaga cggccgtgaa cttggcctga cgggcatgat tgctgatatg
      841 ctgaaacgtt ctgattttca ggttttgccg gataaatttg aacctcatgc agacaaactg
      901 gttacggaaa acgttcagca tattgctcat agtctgactt ggatcgaaca aaacgtgaaa
      961 ggttcacagc tggcacctga aaataaagta gtggaacctc aggaagcagt acaaaaagtt
     1021 ttgaataaac tccactcaca agactctccg gtggagaatt cacaggctcc ggtgattggt
     1081 ttgacaggaa caggaggtgc aggaaagtcg tgtctggcag atgaattgtt gcgaagtttt
     1141 cttgaagaat ttcctgaaaa gcgcatagcg attttgtcag tcgatccctc caaacgcaaa
     1201 accggtggag cactgcttgg tgaccggatg cgtatgaatg ccatcaacga tccccgtgtt
     1261 tacatgcgct cactggccac acgccgttcc cacctggcca cttctactgc actggaaggt
     1321 gctgttagct tactgcaggc aaccggaaaa tcaggaggcg gaggatttga tttgattttg
     1381 gtggaaactg ccggcatcgg tcagagcgac tccgagattt cggattttgt tgatttgagt
     1441 gtttatgtga tgacaccgga atttggtgcg gctactcaac tggaaaagat tgatatgatt
     1501 gactttgccg attgtattgt aatcaacaaa tttgacaaac ccggagcaca ggatgctttg
     1561 ctggccgtgg gcaaacagta taaacgcagt cgaaatgact ttgatgcaac tactgaaaca
     1621 atgccggttt ttggtgtggt tgccaatcgt ttcaacgaca gcggcacaaa ctggttttat
     1681 cacaagttga tcgaaatcct gatcaagaaa aattcactta actggaaacg cagtcatgaa
     1741 gctgaatttg ctgtttccga aattacagag ttgataccgt cggaccgtaa agaatattta
     1801 cggcaaatcg ccggctgcgt acgtagctac aaaaaagaag tccggaaaaa cgcacagact
     1861 gccgcggaat gcggacaatt gcacggaacg cttaaacaaa tgctggggga aaccgaaaat
     1921 aaaaatagct ggaacatact ccagccctgc ccgattgaaa atattccgga aactgctcat
     1981 acagtggccg aactgtacaa tgaaaaaacc tcaaaacttc cggaatcact gcggctgcaa
     2041 ctgagtgaat ttcatgaaaa gaaccaggcg tatctgggcg aaaatttcag ctttgatatt
     2101 cgcaacaaag aactgaaaat ttcgaatcac aacatcagtc tttccggact caaaattcct
     2161 aaaatcgcca cacccaaatt taacgactgg ggcgagattg caagctggtt gggattggaa
     2221 aattttcccg gaagttttcc gtttaccggt ggtgtttttc ctttcaaacg tgaaggcgaa
     2281 gacccgaccc gcatgtttgc cggagaagga attgcggagc gtacaaaccg gcgttttcat
     2341 ttgctggcct gcgatcagcc tgcaacacgc ttgagtacag cgtttgactc ggtgacgctt
     2401 tatggttcaa atccgcattt gcgacccgat atttttggaa aaatcggaaa tgccggagtt
     2461 tcaatttgca cagttgatga cgcgaaacga ctatattctg gttttgatct gcttttaccg
     2521 aagacttccg tttccatgac aatcaacgga cctgctccag tggttctcgc ttttttcatg
     2581 aatgcagcca ttgatcagca aatagaaaaa catttacgtg aaaacgggca tttagaaaaa
     2641 gcgcaaaaaa cgcttcaaga acgctacaaa aaacagggtg tggagcctcc ggagtatcgt
     2701 atggaacgtc cggaaaatca cgatggactg ggtttggaat tgttgggatt gtccggtaaa
     2761 cattttgtag ataatgaaac ttacggtgct ataaaggctg atgtgttaaa caacgtccgc
     2821 ggaacggttc aggcagatat tttgaaggaa gaccaggcgc aaaacacttg tattttcagc
     2881 acaaattttg ctttgcggat gatgggtgac attcaggaat attttaccgc aaacgatgtc
     2941 cgtaactttt attcggtaag catttccgga tatcatattg ctgaagccgg agcaaatccg
     3001 atcagccagg ttgccttcac acttgctaat ggatttactc tgattgagta ttatctggca
     3061 cgcggcttgg cgattgacga ttttgcgcgt aacctgagct tctttttcag taacggaatg
     3121 gatccggaat atgctgtaat tggaagggta gcacgcagaa tttttgctgt cacactgcgt
     3181 aatttgtatg gagcaaatga acgctcacaa aaattaaaat atcacattca aacttccgga
     3241 cgttccctgc atgcaatgga gatggatttc aatgacatcc ggacaacact tcaggcactt
     3301 tatgccgttt acgataactg taattcactt cacaccaacg cttacgacga agcggttaca
     3361 actccaacgg gtgagtcagt tcgtcgtgca ctcgctattc agctcattat caatcgtgaa
     3421 ttaggacagg cattcaacca aaatccaatg cagggcagtt ttctgattga ggaattgact
     3481 gaattagttg aagaagcaat tttgtcggaa tttgtcagac tttccgaacg aggaggagtt
     3541 ttgggtgcaa tggaaacgat gtaccagcgt aacaaaattc aggaggaatc actttattac
     3601 gaaactctta aacattccgg agaactgcct ataatgggcg tgaacacttt tcttaaccca
     3661 aatcctgctg aaactgatgc agaaattgaa ctggcgcgtt caacggaaga agaaaagacc
     3721 atgcaaatcc atgatctgga gaattttcac aagttccact ccaaagaacg agactcaatg
     3781 atggaacgtc tcagagaggc ggcactaaaa aatggcaata tttttgaggt cctgatggag
     3841 tctgtgcaag tttgttcatt gggacaaatc actcaacagc tttaccaaat cggcggacag
     3901 taccgtagga atatgtaagc cctgaattga cactaaaacg attcattctg atttagtgac
     3961 aaatgatata atgctatctg ccctcaaatt aagaccacgt tttgttccag cgtaaaagta
     4021 tttacacctg cattaaaaac aaacaatttt tcaaatctga attcagcggg ggaattattt
     4081 ataattcttc ttagagcctg accatttgat gcaaacttgt ttttctagcc ggcttaaaat
     4141 ttagtactcg gggttttgtc cagtatttct caaaaaccaa ctacaaaaat cttgttcctg
     4201 aaacagcaca gaaaaatcaa attgaagttt cacgacacct cgcagattcg atttatctca
     4261 gacgatgtaa ttactccatg acgcaaaact tcaaaatttt tgttttggca atttaagata
     4321 gtcgaggccc gattttcttc cagttctcct ccatcaataa tcacatcaac cttgttgttc
     4381 aactgattat ggacttctgt tgcagaagag cattccggca tgcctgtgag gttggcgctt
     4441 gtaccaatta ttggacaact tcccaattct atcaggcact gtgcagctgg agcactcgtg
     4501 taacgtacgg ctaaagttcc gtcactgttt tgaaggtgtt gcggaagttc cggattcgcg
     4561 gctaaaatca aggtcagtgg gcccggccaa aacgcattca ttaaatcatc aatccgagag
     4621 tcgttccaca gacaaagacc tggcagccat tccggtgtag tcagcacaag caacggttta
     4681 tttctgggcc gttccttaat gttataaaca cgatctgcaa cttctttaaa aaacgcattt
     4741 ccacccaaac cataaaaggt ttcagtagga aaagccagaa ccttattatg caaaagtgct
     4801 gttttaacca atatttcttc tgtttttgaa aaagggaaat taactaatcg tgctatattt
     4861 tccatccttg cccttgatta gcagtattgt aatcaagctc tagaagggcc tgtaataccg
     4921 tcctatgatt ttttgacgtc ttgtcaggct ttaatcccgc cctgttaagt ctgagagctt
     4981 gtccggcata taatcccaac cgtgactgtg atccttgtca tttgttttat ttaccatcaa
     5041 tccacccgga caagtggctt acccttccgt atgtaattcg tgaaacacga aatgtggtca
     5101 gagccaggtg gttacgtgaa tcgaggtata ccggaggtaa caactttccc tgccattttg
     5161 ttatactttc agcagcagaa actgcttttt gtctactcaa agaacgtccc gttcgattta
     5221 agacttcaac cattaattct gctactgcat ggccataaag agtccagcgg ttgagagcca
     5281 ggtttggcgc atattcactg agtattagct tatgtaatat ttgaccagga tgttctgttt
     5341 ctattttaag gggatatgct gtcaaaacac gtactcgttc agaaactttt tggcccagcc
     5401 actcagggag tcgactatcc gcaagcgcgt gtcctgttaa catgggaata tttatctgtg
     5461 gagaaaactt caagaagttc ataacatcag tataatttcc caatacaacc agaaaatctg
     5521 gattattcct acgaatcagc tgccattctg cgctaagccg tcctgctttt cccggcagca
     5581 gtttggcaga aacgctatac aattctttgg tcaaggcttt tactgccctc tgatatacag
     5641 gcttttccgg ataccagatg ataaactctt ttccagtgtg cttttctgta atatattttc
     5701 caataacacg cgcttcggtt tcagcagtag gcataaaacc aaagacattt gcacgaatgg
     5761 gttgtgtcca ttccggcaaa tctgagccga caaaaaaact tggcagtctt tgctgtttta
     5821 acaaggggta aactgcttga tgagttttac tgccaatacc gctgaaaaca gcaaatatgt
     5881 cattttttaa agtcagttcc tccagttcct cttttgcttt gtatggtttc aactgatcat
     5941 caattatttt caactcaatt gaacgaccgt gtactccgcc ctgatcattg acatatttaa
     6001 aataggcctg cgcgcttttt ccaatatcag aatattcctt tgcaggtcca ctcaatggct
     6061 gatgagttac aaaaacaatg ttttttggaa atactccggt ttcggcaaaa cagaaatgag
     6121 caattgaaaa taaaaacaca agtatgacgg tcagtcgaaa cagtttcata tttttcgtgt
     6181 atatttaatt aactcgacag tcacgagttt tatcatgtca acccaaacgt atgtgaagga
     6241 tcttataagg taacagcact ttatcagatc tttcctttcg gtcgagatgt cattcagcag
     6301 tcaataatat gagtgtctta tgaaatttaa tggctctcgc cttaattaat tgtgataaac
     6361 taaaaaaaaa taggcaacac atgtttgaca ttcattaaca gccaataagc tattatatca
     6421 taaacttaca tgttcagtaa ttttcattcc acaatcaatt cgataactag tgttgtcggg
     6481 gttacaacct gttactaaag catcaataac agcttattat tttagctcaa aacaaaggag
     6541 ctgcacccaa ttctgacagt tgatgcagta aaccggtaaa acggcgtgtt gaaccttcat
     6601 tttcaacggc cttcttaata gctgcatctg ttccaatcaa aacaactaac tgtttcccgc
     6661 gggtgattgc agtgtagagt aaattccgct gaagcatcat ataatggtgt gtagtgagag
     6721 gaacaatcac tgctgaatat tcgcttccct gggatttgtg aaccgtgatt gcgtaggcca
     6781 gagatagttg gtccatctct ttcccctggt aatgaacaat ttgctgatca aatttaacat
     6841 gcagttcttt ggtttttgga tcgcaattaa caattcttcc aagatcgccg ttgaaaacca
     6901 gtttctcata gtcattttgc tgctgcatca ctttgtctcc aatccggaac cactgttcac
     6961 gatgttccag accttttgca tctggattta ttacatcctg cagtttccgg tttagattta
     7021 ttgcacctgt gactccacgg tgcatcggcg tcagcacctg aatatccatc atcggatcaa
     7081 gctcgaaacg ctggggaatc ctttcggtac tcatcagcag tattttttca acaatttttt
     7141 ccggattcga ctctttaata aaataaaaat ccagcagctg atcatcatcc tcggtttgcc
     7201 gatttgagct actgaagtcc ggcatttctc cccgacgaac tttgtgagca ttgactgtaa
     7261 taaaactatc agctgcctgc cgaaaaatat gatcgaggcg cacaacggga attgatctgg
     7321 attcaatcaa atccgacaac actgctcctg cacccactga cggcaattga tctacatcgc
     7381 cgactagaat caaacgtgac gcagatggaa cggcttccag taaactatcc atcagcagcg
     7441 tatcgatcat tgaagtttca tcaaggatca gtaattcctg ctccagagga ttttcccggt
     7501 cacgctgaaa acctatgttg cttgcttcaa gaagacggtg aattgtggaa gctgcagcgc
     7561 cggtagtttc agtgattcgt tttgcagcac gtccagtagg tgcagcaagt gcaattgcag
     7621 ggattctggt gcgcatcagt ccaagtataa agcgcacaat ggttgtttta cctgtgccgg
     7681 ggccaccggt tataatcagc actttatgct gaagagccgc ttcaactgcc tctctctggg
     7741 caggatcaag cttcaggcca acttttcgtt cctgctcttc tatcagataa gattcaccct
     7801 cataaaccgt ataggcttca ctgttaagga ttcgaaaaat attctccgca acacgccgtt
     7861 ctgctttgta aaatctcgga cggtaaacca attcctgctc aataccggaa gcatcttcaa
     7921 ttttctcact attcaacaaa cgatcttcca gcaattgccg aattgatgac tccagcattg
     7981 atgagtcaat attaagctct tgcacggttt tttcgagcaa atcccgacga gggaaacagg
     8041 tgtgaccatt gagtgcctgt tgttccagca tatagagtaa gcctgcgtca gcacgatgcg
     8101 gagagttatt atcgaatccc agattgcgtg caataccatc tgcagtaaga aaacctattc
     8161 ccggaatatc agttaactga tacggattgg ctttgatcaa cggaatactg ttcaagccat
     8221 tcttcgtaaa aatacgttgt gcatatgcgt gtgatattcc tactccacgc aaaaaagtca
     8281 ttacatcccg gagacctctt tgatcatccc acgcctcaag cagagatttt tgttgttttc
     8341 tgccgacgcc tttaactttc gataataatt ttggagaact gtctataatt ttaaaagtat
     8401 catttccgaa ctttttgaca attcgctctg ccgttttttt accaattccc ttaaatactt
     8461 cagaagaaag gtagcgtacc atcccttctt cagaggttgg ttcaacaggc atgaattcac
     8521 gaatcgaaaa ttgacgacca taaattttgt gattctccca gctgcctttt aaacgtaatg
     8581 tttcatgctc atgggtgttg aaaacaatgc cttttacagt gatcggcctg atggaatttt
     8641 ctgttagaaa tgtaccaatc aaaaatccgt tttccggatt tgtgtacaaa attttttcca
     8701 gagtaccgac taagctaaca aagtcttcgg ccattggttt ttcaggaagt gtaataatta
     8761 aatttatgtc aaattagtat cagaatggga atcaaaccag caagtttttt ccatgaggtc
     8821 actttgttga aaaagggact caaaaaacag aagtgcatgg ctttcgggct tttacaggat
     8881 aaaccttgca gtagtcatca gcaatcgaac aattactttt tggttttatt gaataatatt
     8941 atattccagc aagtactaat ttggtggaat ataaaaagga gaccggatca tctgtagtaa
     9001 atcgaagcgc cactttccac tttccttcag cagcaaaaat gattgctgct ttgtaaattg
     9061 taaatcaaaa atgcttttgt gtcgaaaaac aattgcagca ttaacataaa gcaatgtcag
     9121 gtatcgatat gatatttcag gttcttttcc agttttcaaa ccaaccggaa cccagtccag
     9181 ttcaaccatt cgcgttgcaa attcttcaat gctaatttga tctgtggagt gttttttgcc
     9241 gtatttgtat aattcaaaat agcgttcctc ctgccagagt tgcagaaagt tcagaaacac
     9301 aaccctggtt tctttacgga ttgcctccaa ttcaaaaggt gtaggttgac gggcaattgc
     9361 cccaggaaca gctataaaaa aactgagtac gcagaacaaa aatagtcgtc tgaataattt
     9421 atttttcgaa aatatcagct tattcaaatt tcccgattta taattcatac agctctacgg
     9481 tacatgtgca aacatagaga ttcaactgca agtgacttat ttgcgttaag ttcaatggtt
     9541 cttcttgatt ctgctatcca gccaaaaatt tcgaaaattt gctgcagagt aaaatgctct
     9601 ccactttttc tcaatgcaga aatgcggttt tcatcagaac caactgtccg gtttatcagt
     9661 ttttcttccg gaagaccacg aaacagccat gttaaatcac gaaaccaagt ttccagaaaa
     9721 tcaagcagca aacgccattc ctcatttttg gatttagccc aggtttcaca ggtccgtagc
     9781 atctcttcca gtgttgctgc agaaaaagtg gtcagccagc ggatggccgt ttgctggaca
     9841 gtttccataa gttcaagacg ttcagcaaga tttccattta cgcttcccat actgaaggca
     9901 ctgagcaact gaatccgggc tgttgacagg ttcgttgttt cccgcaatat gcgttccgaa
     9961 atttcctcag acaatggacg aaatcgtatt tgttggcaac gcgaaacaat tgtttccagc
    10021 agctgttgtg atgaatcagc aatcaaaatc agcaaagttt ttggtgccgg ttcctccaac
    10081 gtttttaaaa aagcattagc tgcttcccgg cccaaatgct gtgctccatc aagaatcaat
    10141 acgcgttttt ttgcctgatc cggatgaagg tgaagccagt tcaatgattt acggatttga
    10201 tcgatcttaa taaacttgcc gtcaggaatc agaacctgga aatccagaaa attctgttct
    10261 gcaatttgaa aacaaatatc acattctcca caagcgtctt ctccacattt tctgcaatta
    10321 agtttttgtg caagagcaac agctgttttg tatttaccga tatttgcctg gcctgtgaac
    10381 aaccatgcat gaggcatcct gtccctctga acggcttgat tcatcaggtc aattgcgtgc
    10441 tcttgtccgt aaatttctct aaacggcatt gtgctatcag gaactgaaaa gtggagaaaa
    10501 gctgacatta aaagcaaaac cgagataaat ggcgattcca atgatgtcgc tggttgtagt
    10561 aataaaaggc cctgttgcta tagcaggatc gatattcatt cgtttgagta taattggaat
    10621 tgttgaacca actgctgctg caaaacaaac aacgatcaat aatgaaaatc cggatgtaaa
    10681 ggccctccca acatcatcag tcaggtacca tgacatgcct gccagtatga aagaacaaat
    10741 aattccattc aaaaatgcga cccttaactc tttccagagt cgaacaagaa tatcagtagt
    10801 ttgtattgcc cctgtggcaa gtccacgcac aacaattgaa gaagactgta tgccgatact
    10861 tcctccaagt gctgctacaa gaggaataaa ataagttaca tccggtagcg aaataagagc
    10921 attttcatac aaactcatca ctatagcagt taaaaaacct ccaaacaaac caaccagcag
    10981 ccagggcaac ctatcacctg aagctcgtaa aaccgatgtc tcactaacct cttcgtcccc
    11041 tgtaccggca atatgaccca tatcttcatt atattcatca acaatcacat ccaccaaatc
    11101 atccagagta atacggccga ttagacgcaa atgcttatct attaccggaa ccactaccaa
    11161 atcatattcc tgcgcaattc gtgcaacttc ctcctgatcc aaatcctgat caacagcaac
    11221 cacttcagga ttcatcaaat tttcaattaa tttatggcgc tccgccaaca aaagttcagt
    11281 cgtaccaatt gtaccaatca agtgatcatg atcatcgaca acgtaagctg tataaaataa
    11341 ttcgaaaccc tcctttttaa caaatttttg tatttctttg attgctgtcg caaccgtttg
    11401 gtctctacgg atcgccacaa catagggcgt catgagtccg cctgcacttt cttcatcata
    11461 ttgaagaaga ttggtcagtt cttcacgatc tttttccggc aatttctcaa gcaacgcttc
    11521 tgctttttca tcatccaaca gaccaacaaa gtcagccgca tcgtcctgcg gcatatcctc
    11581 gattgcatta ctcaatcgat cagtagatat ttgttctaca atgtcgccaa gaatggaagg
    11641 tgaatcctgc agattgttta gcacctctga agacgtttcc cgatccagat tttccagtaa
    11701 taccaactgt tgcttcaatt ttaactgcag cagaatgttg gcaatttcca caggtttaag
    11761 cggttcaagt atgcttttca gcaactgtgt ccgttccgtg ttgatcagtt tattgaccag
    11821 cttatggatt tgtgtgtcgc gacttttagt catgattata tctgcaaaaa atccaattat
    11881 atctgcactt atttaagcgg ttgccattcc tgcacaaaat ctacaagcag tctcaccccg
    11941 acaccggtag ccccactgtt gggctggtaa ccgagatgtt ttacattcca ggatgttcct
    12001 gctatatcaa gatggaccca aggtgtttta tctacaaaat gttctaaaaa cagacctcca
    12061 actatgactc cggaatcacc tccgccaata ttttgcaggt ctgcatattt tcccttgatg
    12121 gattcgccgt attcggggaa gcttggaaac tgccagaccc ggtcaccact aatatctcct
    12181 gctctacgta cttgttccat caaggcatca ttgttcgaga ctgctccagt agtgaggtgt
    12241 cctagtgcag caacacatgc ccctgtgaga gttgccaggt cgataaccgc atcaggtttg
    12301 tactccttaa caacataact aagtgcatca ccaagaatca gtcggccttc tgcatctgtg
    12361 ttgaggattt caacccgttt gccgttgtgt gctgtaacta tgtctcccgg acgttgggca
    12421 ttgccgccag gcatattttc cgttgttgga ataacagcaa taatatttag ttttgggtta
    12481 acctgtccta tgactttcat tgcacccaaa acggctgcag caccgcacat atcgaatttc
    12541 atttcgtcca tatttttccc aggcttgagt gaaattccac ctgaatcaaa tgttacgcct
    12601 tttccaacaa ttgcaactgt ttgtttggcc tgctgatgag catactccaa aataattaat
    12661 ttagccggtt cccgggaacc tcttgacaca ccaagcagca tctccatacc cagttttttc
    12721 atgtcttttt cttccaaaac cgtgcattcc attttgtatt ttacagcaat cctttttgcc
    12781 tcagccgcca gatcttttgg tttcaaatca tttgcgggcg tattacctaa tgtgcgggcc
    12841 agatttgtcc cgtcagcacg tattttcccc ttttttaata ctttcaatga agtaagtttt
    12901 tccgggtcac aacatttaat cgtaattgat ctggagtttt gtttttcatt ttgtgatttt
    12961 ttgtaagtgt taaactcata ggcacctaaa gttaaacctt ctaccaggac ctgtcctgca
    13021 tctacttcaa ccgcattttg aataagtgag gtcacaacaa atcccacctt atttgctttc
    13081 agtaaagtca gcatttttcc cgcttttcct gcagcacgtc tcaacgtctc tgcatctaat
    13141 tcatcttttt tacccagacc aaccgctaaa atttgcggga tttttgcata accagaaaac
    13201 aaatgctgac ttttgtcttt cttaccggta aaaactttca atttaattga ggcgttcagg
    13261 ttttttttga gatctccagg aagggacctt agtccacttc cagaaaggtc tgagtcttca
    13321 acgacaaata cgaccagagc ttccagtttt tcacttgtga gagaatcatt actgtattca
    13381 atttttagtt ccattgttaa aactttttat aaaaagatga taattcagca cccaactact
    13441 aaaaacacgg tatttaagtg tcgggttaat ttttttcagt aatcaccata cccagcctgc
    13501 agagaaacag acaagacttt agtgtaaaag gcgttaattc attaccagag agattgcagt
    13561 ctgcgtccgc cacttataag ataatctgca tcaattccaa gatatcttaa atgattaaag
    13621 gcagaccaga tgacaacagc aaaacaaaga gtcagtaaaa aaactgtcat aaacataaac
    13681 tattaaaaag ttggcaaaca attttcatca agcagcttta gttaatataa aagttgacat
    13741 agtcagtaag agactataga tttcgctgat gaattttact ttttacttat acttgat
//
LOCUS       DTVD01000305           13712 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_955_length_13712_cov_329.267482, whole genome
            shotgun sequence.
ACCESSION   DTVD01000305 DTVD01000000
VERSION     DTVD01000305.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_13712_cov_329.267482"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            467..4756
                     /gene="rpoB"
                     /locus_tag="EYO68_11115"
     CDS             467..4756
                     /gene="rpoB"
                     /locus_tag="EYO68_11115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIC08393.1"
                     /translation="MPSTLENRHIIRRSFGSLTAPIKVPHLLKVQLDSFERFLQRDVP
                     PDQREEKGLESVFRSVFPVSDFSGSSTLEYVHYRLGDPKYSVEECRVRGVTFACPVRA
                     ALRLIVWEKDSESEVKHIRDIKEQDIYLGELPLMTPTGSFIINGTERVIVSQMHKSPG
                     VVFTHDKGKSHSSGKLLFSARVIPQRGSWLDFEFDAKDILYVRIDRRRKFHATILLKA
                     LGNTEEKLLEYFYPFEKLDLSEVKPGEDPEAQYYYRVLDPEILLNQRPQLPIADPKTG
                     EVLVKSGQRINKRLLKKLENAKVKRLNVTLHELKGRIIARTLYKAGSEEILVPCNTAL
                     TAELLTTLVENDIKEVELLHIGPQNMGSTLSDTLALDKVTSPEQALIELYKKMKPGDP
                     PTLEAAQMMLQNFFFKKERFSLSKVGRLKINQKLGLDDPLDNTVLTKVDIMETVKYLL
                     ELKEGLPNRMIDDIDHLGNRRVRSVGELLETQFRIGLVRMERTIKERMSLQDSDSMML
                     HDIVNAKPVAGAIHEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFDV
                     RDVHSSHYGRICPIETPEGPNIGLIASLASFGRINDFGFIETPYLKVENGVVTDTVEY
                     LSAIEEEKYSIAQANARLDKKKAFINDFITSRVGSDFSMVLKENIDYIDISPCQLVSV
                     AASMIPFLEHDDANRALMGSNMQRQGVPLVKPKAPLVGTGIEHQAALDSGSCVVASRS
                     GVVDNVDSGRIVIQADVDLSSEDSIVPANVDIYHLIKFRRSNQNTCINQRPIVKKGDR
                     IKAGDVIADGSCTENGELALGQNINIAFMPWLGYNFEDSIMVSQRLMHEDTFTSVHID
                     VLDTVARDTKLGKEEITRDLPNVSEEALKNLDESGIIAVGTSVSSNEILVGKVTPKGE
                     TQLNPEEKLLRAIFGEKAGDVRDTSLRVPQGVDGVVTNVVVFNREGVERDERTRQIEQ
                     ELLSSYEKDHHDEIRIVHNNLVNRILSVAEKKPLSADVLSIQGEVLVKKGTKISEEIL
                     HKIPLKSADGIQVTDKNINLKVGTFVRNALQQMYLLENVYQDRCEKVSKGDDLPPGVI
                     RMIKVFIAIKRKLSVGDKMAGRHGNKGVVSTIQPVENMPFMEDGSPVDIVLNPLGVPS
                     RMNVGQVLETHLGRVASGLGKKIEEFLEKNNPAKQIRSFLKNVYECQVAQKYFDSMDD
                     QTFLEFVQKYKPGIHMATPVFDGAKESDIHRMAEMAGLSKSGQVWLYDGMTGERFSQK
                     VTVGYAYILKLHHLVDEKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAYTLQELLTVKSDDVHGRNKIYESIVKGRHQLEPGIPESFKVLISELKSLCLNIELL
                     QNSEEEEEEIPEGMESSPASEEVTGNKAEEKISKAVSEESNEEIDTAEVTA"
     gene            4800..9083
                     /gene="rpoC"
                     /locus_tag="EYO68_11120"
     CDS             4800..9083
                     /gene="rpoC"
                     /locus_tag="EYO68_11120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIC08394.1"
                     /translation="MALRDLFEIAVNPKNYSAIRIRIATADEVTSWSHGEVRTSETIN
                     YRTFKPERDGLFCGKIFGPVHDYECICGKYKRMKHRGITCEKCGVEVIESKVRRDRMG
                     NIKLACPVSHVWFLKGVPSRIATILDMMLRDLERVLYFDAYIVLEPGDSELEELELVE
                     EDQYRELEENHPDLKLGMGAEAVQTLLRRIDLAELEKQLQIDMREVNSETRRKRIAKR
                     LIVVSKLLKSGNAVDSMIIENLPVLPPELRPLVPLEGGRFATSDLNDLYRRVIHRNNR
                     LKRLIELRAPGIIVKNEKRMLQESVDALFDNGRRGRPMVGSNKRPLKSISDMLKGKQG
                     RFRQNLLGKRVDYSGRTVIVVGPELKLHQCGLPKKMALELFKPFIFHKLIDYQEIHTI
                     KNAKKVLEENPPEVWAILEEVVREHPVLLNRAPTLHRLGIQAFEPILIEGKAIQLHPL
                     VCTAFNADFDGDQMAVHVPLSVEAQLEAKILMMSTNNILSPANGKPVMTPTQDMVLGL
                     YWITREREGALGEGKIFSSREEVLVAFEHGRVDLHAKIKVRLEHLGEILSEENNQETS
                     TSKPIVETTVGRIILSMVIPEEVPFKSINVHLKKKQMAELVDESYRKAGSVKSVKMLD
                     DLKNLGYQYATKAGFSISMDDMVIPKEKTTQLKKAQVDVNKINMQHQDGLITDGERYN
                     QVIDIWARTTEKISEKMSAGLSEEIIDEEGVHKVNSIFMMADSGARGSNQQMKQLAGM
                     RGLMAKPSGEIIETPIHANFREGLNVLEYFISTHGARKGLADTALKTANSGYLTRRLV
                     DVSQDSVVLEDDCGTLDGIEMTALIESGEIIESLGDRILGRVLLDDVLDPNVENEILI
                     PAGTLIDESDRDVIENSRIEKVQIRSPLTCMSEQGVCRNCYGRDLCHGGLVNLGETVG
                     VIAAQSIGEPGTQLTMRTFHIGGTASRSAEQNKWEAGFSGVLRFDDLKEVKNREGNFV
                     VLGRRGEAVIVEGGKNIAVSKLADLENERERERRPLPMGATLLKKEGELVEQGDLIAE
                     WDPFSIPIITDVSGFVNFEDINEGETFKEQVDEVSGVSRKVIHESQSLEQHPLIAICD
                     SKKKTIIRKNDTATEFALPNKSELVVENGLEVHAGDVLAKIPRELARNKDITGGLPRV
                     AELFEARVPKDQAIITEIDGIVEFDLDVKKKQRIRIRPEDGKGDSKEYLIPRGRHITV
                     HMGEYLRAGDPLIDGSPNPHDILAVKGSKALQKYLVDEVQQVYRLQGVGINDKHIEVI
                     VRQMLRKAYVEDPGDSSLLVGQEIGRSELNSINQELTAQGLRPARSKPKLLGITKASL
                     STNSFISAASFQDTTKVLTDASLAGKHDTFRGLKENVILGRLIPAGTGFNVFNNMDYD
                     LGEGVIDPAAMRKAADEAARAAMVREITEAEKNKKPADLVAEEKIPEAA"
     gene            9112..9546
                     /locus_tag="EYO68_11125"
     CDS             9112..9546
                     /locus_tag="EYO68_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIC08395.1"
                     /translation="MLPSIETSHFELKLSVRDYELDSFGVINNAVYQNYLEHTRHQFL
                     ESQGLKLTAYFEQGLSPVITKAEIEYRSSLQSRDDFVVSLELALLSKVKFIFLQEIRS
                     IPDKTLILSARITGTILNSSGRPFLPAAFQNLRTLLTSKTKN"
     gene            9562..10239
                     /gene="rpiA"
                     /locus_tag="EYO68_11130"
     CDS             9562..10239
                     /gene="rpiA"
                     /locus_tag="EYO68_11130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIC08396.1"
                     /translation="MISEKQRSAEAACEYVKDGMIVGLGTGSTAEFAVKKIGELVRNG
                     LSIRGIPTSDATKILAESEGIPLIDFSETMFIDLTIDGADEIDANLNMIKGGGAALLR
                     EKIVASASREEIIIVSGSKLVQQLGAFPLPVEVIPFGWQVVFNQLESLQGNPELRLKQ
                     GQPSVTDQGNFIVDCHFRKIENPEQLEQHLNMIPGVVENGLFINLCTRMIMADGENII
                     IKNRNLN"
     gene            10347..11357
                     /gene="gap"
                     /locus_tag="EYO68_11135"
     CDS             10347..11357
                     /gene="gap"
                     /locus_tag="EYO68_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIC08397.1"
                     /translation="MTIRIAINGFGRIGRMVVRAANKNQNVEIVAINDLVPSENLAYL
                     LKYDSTHGRFDGDVQAGQDCLVVDGKTIECLSERNPGDLPWNKMNIDYVIESTGLFTT
                     SEKAEEHLKAGAKKVVISAPAKSAEIKTLVMGVNNETYDPGTDNIVSNASCTTNCLAP
                     IVKVVLDNYGIEEGLMTTVHSVTAAQPTVDGPSKKDWRGGRGGPQNIIPASTGAAKAV
                     ALCIPEIAGKLTGMSFRVPTPNVSVVDLTVKLSKSTSYEEINSSMKAASEGKLKGILG
                     FTDEDVVSSDFIGSTYSSIYDAGAGIGLNDRFFKLVSWYDNEMGYSTRLIDLIEYMEG
                     QN"
     gene            11480..12313
                     /locus_tag="EYO68_11140"
     CDS             11480..12313
                     /locus_tag="EYO68_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIC08398.1"
                     /translation="MHKVELKEVGLDIGLAFARHFYKTDYLHYGYWPEALSVDPANVL
                     EAQENYANLLLKHIPDGVNSILDVGCGSGKFSEKLLDAGYTVDCVSPSPNLTKHVRER
                     LGERVEIFECRYEDLKTKKYYDLILCSESFQYLLLEKAMAQTQELLNQKGHLLICDFF
                     KRDVEGKSPVSGGHKIERFLKYMQAQPFRQITDIDITKETAPSLDIMRDLIQEVIAPT
                     WNIVAYYMTSNYPRFSKLFSFAFRKKIAQANRKYLSGGTSGEQFAHFKTYRLFLYQKN
                     S"
     gene            12402..13685
                     /locus_tag="EYO68_11145"
     CDS             12402..13685
                     /locus_tag="EYO68_11145"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIC08399.1"
                     /translation="MSDLETKNAIRMILLTASLGGMSYLSFNNGLLLSYFSHLGIPSA
                     TILILLSILPLTIFLVIIPFSYFSDIFGKKRIGIIGMVFSFFGFALLIGVSYLPDSLR
                     IMAVGFGIVVFGIGSAMTLGNWFALLHPIIPENIRGRFFGQLRLTWQSIGILFTLIVI
                     YILELYPTLGMYQAILGVITILMLLRMFFYQQIPELDKSTVKKESFRISFLKVLNIPG
                     YLPFCAYCFLLTLFTGACPQIFNLIEKDVLLWSKVEIVFIGNLLVAGALAGFFLGGRM
                     IDRFGTKYVFLCCHFGFGIILLLFLWRNLFTEEIRIVVGILTILFGMVQAASSIAMTS
                     ETLALIPPKNKSLATGLWFTLYSGGTGLSGILSGQALELGVFNESWIWLGQSMSSYDG
                     LLLLSGTMVLLMTVTLGMIPSMIKKVPAGWIPQSS"
BASE COUNT         4171 a         2573 c         3111 g         3857 t
ORIGIN      
        1 aatctcctag ttccttaatt gcaaattcgc tgcaatctga acatcagtta tttagcttca
       61 ataatctctg cacctggaag agtaaaaggc gttctgcatt attattagca ttaaatataa
      121 aattattaaa aatatatttg ctttttttat tataaagaaa tatattaagg ggttagattt
      181 acgaagccgc aatttgcgga aagcttaatc aaaccccctc cacttgaacc ttggccattt
      241 tccacatgca aattgtctta aacgaccatc agcgctgtat ttccgctagc aggaaaaaca
      301 ttgggaaagt gcctctggag aatagatcgg gtcagcgctg ttattgacag tcccgaattg
      361 tgttacaaaa cctttctgca gctattctgt ctcaaatgga agtcttcttt cattttgttt
      421 actgcagttt aagaccaacg ataaacccta acctcagtca gttcatatgc cttcaacatt
      481 agaaaatcgt catattattc ggagatcctt cggaagcctt actgctccga ttaaagttcc
      541 acacttgttg aaagttcaat tagattcatt tgagcgtttt cttcaacgag atgtcccacc
      601 tgatcagcgt gaggaaaaag gtcttgaatc agtgttccgc tcagtctttc ctgtttctga
      661 ttttagtgga tcttctactt tggaatatgt tcattaccgt ttgggtgatc ctaaatattc
      721 tgtagaagaa tgccgggtcc gtggagtgac ttttgcctgc ccggtacggg cagctttgcg
      781 tttgattgtt tgggaaaagg attctgaatc agaagtgaag catattcggg atattaaaga
      841 acaggatata tatctgggtg aattgccttt aatgacccct acaggctctt ttattatcaa
      901 tggaactgag cgtgtaattg tcagtcagat gcacaaaagt ccgggtgttg tttttacaca
      961 tgacaagggg aaaagtcact ccagcggaaa actgctcttt tcggcacgtg tgattccgca
     1021 gcgtggttcc tggctcgatt ttgagtttga cgcaaaggac atactttatg ttcgcatcga
     1081 ccgccgccgt aaatttcatg caactattct tttgaaagcg ctgggtaata ccgaagaaaa
     1141 acttctggag tatttctatc cgtttgagaa gctcgattta agcgaagtta agcctggaga
     1201 agatcctgag gctcaatact attaccgtgt tttagacccg gaaattttgc tcaatcagcg
     1261 cccacaactc ccaattgcag acccaaaaac cggtgaggtt ttagtcaaat caggacagcg
     1321 gattaacaag cgtctgttaa aaaaattaga gaatgccaaa gtaaaaaggc ttaatgtaac
     1381 tctccatgaa ctgaaaggac gtatcattgc ccgtactctc tataaagcag gtagtgaaga
     1441 aatattagtt ccatgtaaca cggctttaac ggcagagttg ctaacgacgc tggtagaaaa
     1501 tgatataaaa gaagttgaat tattacatat tggcccacaa aacatgggct ccactcttag
     1561 tgatacattg gcgctggata aagttacttc gccagaacaa gcgctgattg aactctacaa
     1621 aaaaatgaag cctggcgatc ctcccactct ggaggctgca caaatgatgc tgcagaattt
     1681 tttcttcaaa aaagaacgtt tcagcctttc aaaagttggg agattaaaaa ttaatcaaaa
     1741 gttaggactt gatgatccgc tggataatac tgtacttacg aaggtagata tcatggaaac
     1801 tgtcaaatat ctgcttgaat taaaagaagg cctccctaac aggatgatag atgacattga
     1861 tcacctgggg aacaggagag tccggagtgt aggagagctt ctggaaactc aattcaggat
     1921 tgggctggtg cgtatggagc gaaccatcaa ggaacgtatg tctttgcagg attctgactc
     1981 catgatgcta catgatatcg ttaacgcaaa accggtagca ggtgcaattc acgaattttt
     2041 tggaagcagc caactttcac aatttatgga tcaaacaaat ccactttctg aaatcacaca
     2101 caagcgccga ctgagtgctt tagggcctgg aggtttaaca cgggaacgcg caggttttga
     2161 tgtccgagat gttcattctt cacattatgg acggatctgc cccatcgaaa ctccggaagg
     2221 accaaacata ggactgattg cctcactggc ttcttttggc agaatcaatg actttggctt
     2281 cattgagact ccatacctta aagtcgaaaa tggcgtggtt acggatacgg tggaatacct
     2341 ttctgcaata gaggaggaaa aatacagtat tgctcaggct aatgcaaggc ttgacaaaaa
     2401 aaaggcgttt atcaatgatt tcattacctc ccgtgtagga agtgatttca gtatggtttt
     2461 aaaggaaaat attgactaca ttgatatttc tccgtgtcag ttggtttctg ttgccgcttc
     2521 aatgattccc ttccttgagc atgatgatgc aaatcgtgcc ctgatgggtt caaacatgca
     2581 gcgtcaggga gtccctttgg tgaaaccaaa agcccctctc gttggaacag gaattgaaca
     2641 tcaggcagca ttggattctg gcagttgtgt tgttgccagc cgttctggag ttgtagataa
     2701 tgttgattca ggcaggatag ttattcaggc agatgttgat ctttcaagtg aagattcgat
     2761 tgtcccggca aacgttgata tttaccactt gattaaattt cgccgctcaa atcaaaacac
     2821 ctgcattaat caacgtccta ttgtaaaaaa aggagatcga attaaagccg gtgatgtaat
     2881 tgctgacgga tcctgtactg aaaatgggga gctggctttg ggacaaaata tcaacatagc
     2941 tttcatgccc tggcttggtt acaactttga agattcaatc atggtttccc agcgtctgat
     3001 gcatgaagat acctttacat cagttcatat tgatgttctt gatacagttg cccgcgatac
     3061 aaaattagga aaagaagaga tcactcgaga tctcccaaat gtgagtgaag aagcactcaa
     3121 gaaccttgat gaaagcggca ttattgcagt tggaacaagc gttagttcaa atgaaatcct
     3181 ggttggaaaa gtgactccga aaggtgaaac acaactcaat ccggaagaaa aactgcttcg
     3241 agcaatattt ggtgaaaaag ctggtgatgt tcgggacact tcgctgcgtg taccacaggg
     3301 agttgacggt gtagtgacta atgttgtggt atttaaccgt gaaggtgttg aacgtgatga
     3361 acgtaccagg caaattgaac aggaactttt aagcagctat gaaaaagacc accatgatga
     3421 aatccgcata gtgcacaaca acttggtgaa tcgaatatta tctgttgcag aaaaaaaacc
     3481 actgtctgct gatgtactct ccattcaggg agaggtgctt gtaaaaaaag gcactaaaat
     3541 cagtgaagaa attctacaca aaattccgct taaatctgca gacggaattc aagttactga
     3601 taagaatatt aacctgaaag tcggaacatt tgtgcggaat gctcttcagc aaatgtattt
     3661 actggaaaat gtttatcagg atcgttgtga aaaagtttcc aagggagatg atctccctcc
     3721 aggggtgatt cgtatgataa aagtatttat cgccataaaa aggaagcttt ctgtaggtga
     3781 caaaatggca ggacgtcatg gtaacaaagg cgttgtttca accattcagc ctgttgaaaa
     3841 tatgcctttt atggaagacg gctcacctgt tgacatagtc ctaaatcctc ttggagttcc
     3901 ctcccgaatg aacgttgggc aggtacttga aacacatctt gggcgtgtag caagcggact
     3961 cggtaaaaag atagaggaat ttcttgagaa aaataatcct gcaaagcaaa ttcggagttt
     4021 cctgaaaaat gtctacgaat gtcaggttgc acaaaaatat tttgattcaa tggatgatca
     4081 aacgtttctg gagtttgtgc aaaaatacaa acccggaatc catatggcta ctccggtttt
     4141 tgatggagca aaagagtccg atattcatcg aatggcagaa atggcaggat tatcaaaatc
     4201 cggacaagtc tggctttatg atggaatgac cggtgaacga ttcagtcaga aagtgacagt
     4261 cggttatgca tacatactga agctgcatca cctggttgat gaaaaaatcc atgcccgttc
     4321 cattgggcct tattctttgg ttactcaaca accattgggt ggtaaagctc aattcggcgg
     4381 ccagcgtttt ggtgaaatgg aggtttgggc tttggaagct tatggggcag cttacactct
     4441 gcaggaattg ctgactgtaa agtctgatga cgtgcatggc aggaacaaga tttatgaatc
     4501 gattgtcaaa ggtcggcatc aattggaacc cggtattccg gaatcattca aagttcttat
     4561 cagtgaactg aaaagcttgt gcctgaacat tgaactactc caaaactcag aggaagaaga
     4621 ggaagaaatc ccggaaggaa tggaatcttc tccggcgtca gaagaagtaa cagggaataa
     4681 agctgaagaa aaaatatcga aagcagtatc tgaagagtca aatgaagaaa tcgatacggc
     4741 agaagttaca gcataatttc tcaatcaaaa ccctataacc aattaaccag caactaaaca
     4801 tggcactcag agatttgttt gaaattgcag taaatccgaa aaattactcc gcgatacgga
     4861 tcagaattgc cactgccgat gaagtaacat cctggtctca tggagaagtc cgaacctccg
     4921 aaacaatcaa ctacaggaca tttaagccgg aacgagatgg actattttgc ggcaaaatat
     4981 ttggacctgt acatgactat gaatgtatct gcggaaaata caaacgcatg aaacaccggg
     5041 ggataacctg tgaaaaatgc ggtgtggaag ttattgaatc caaggtgcgc cgggatcgta
     5101 tgggcaatat aaaactcgcc tgccctgttt ctcacgtctg gtttctcaag ggtgttccca
     5161 gccggattgc aaccattctt gacatgatgt taagggattt agagcgtgtg ctatattttg
     5221 atgcgtatat cgttcttgaa ccaggagatt ctgaactgga agaactggaa ctggtggaag
     5281 aagaccaata tcgggaattg gaagagaatc atccggattt gaagttgggt atgggtgcag
     5341 aagccgtgca gacgcttttg cgaaggatcg atttagccga gctggaaaaa cagctgcaga
     5401 ttgatatgcg ggaagtcaat tctgaaacac gccgcaaaag gattgccaaa cgactcattg
     5461 tggtcagcaa gctcctgaag tctggaaatg cggttgactc catgatcata gaaaatttac
     5521 cagtccttcc tccggaactg agacccttgg ttcctctaga aggcggacgc tttgcgacaa
     5581 gtgacctcaa tgatttatat cgacgtgtta ttcacagaaa caaccgtctc aaacgactga
     5641 ttgaattacg tgcacctgga attattgtta aaaatgaaaa gagaatgtta caggaatctg
     5701 tggatgccct tttcgataat ggccgccgag gccgtcctat ggtgggttcg aataaaaggc
     5761 cgttaaaatc aataagtgat atgctcaaag gtaaacaagg acgtttccgt caaaatctgc
     5821 tgggtaaacg tgttgattat tccggacgta ctgtgattgt ggttggaccg gaactaaaac
     5881 ttcatcaatg cggtttacct aaaaaaatgg ctttggaact tttcaagcca ttcatttttc
     5941 acaaattgat tgattatcag gaaatacaca cgattaaaaa tgcaaagaaa gtgcttgagg
     6001 aaaatccccc tgaagtatgg gccatccttg aagaggttgt acgtgaacat cccgttttac
     6061 tgaaccgtgc gccaaccctg caccgccttg gtattcaggc tttcgaacca attttgattg
     6121 aagggaaagc gatccagctg catccgcttg tttgtacagc attcaatgca gactttgacg
     6181 gtgaccaaat ggcggttcat gtcccccttt ctgtggaagc acaactggaa gcaaaaatat
     6241 tgatgatgtc caccaataat attttatctc cggcaaatgg aaaacctgtg atgacgccaa
     6301 ctcaggatat ggtgcttggt ctttactgga ttaccaggga acgtgaaggc gctcttggag
     6361 aaggaaaaat tttctccagt cgagaagaag ttctagttgc ttttgaacat ggccgtgtgg
     6421 acttgcatgc caaaataaaa gtacgtttgg aacacttagg agaaatattg tctgaagaaa
     6481 ataatcaaga aacatctacc agcaaaccaa ttgtggaaac cacggttggt cgtattattc
     6541 tttctatggt aattccggaa gaggttccat tcaaatcaat caatgtacac cttaagaaaa
     6601 aacaaatggc cgaacttgtt gatgagtcat acagaaaggc aggaagtgtt aaatcagtaa
     6661 aaatgttgga tgatctgaaa aatttaggtt accaatatgc aaccaaggca ggtttttcca
     6721 tcagcatgga tgatatggtg attccaaaag aaaagaccac tcagttgaaa aaagctcaag
     6781 tagatgtcaa caaaatcaac atgcagcacc aggatggttt aatcactgac ggagaacgct
     6841 acaaccaggt aattgatatt tgggcacgga ccacagaaaa aatttctgaa aaaatgtctg
     6901 ccggactttc tgaagaaatc attgatgaag aaggtgttca taaagtgaat tcaattttta
     6961 tgatggcaga ttccggagct cgcggtagta atcagcaaat gaaacagtta gccggtatgc
     7021 gtggtttgat ggcaaaacct tcaggtgaaa ttattgaaac accaattcac gcaaatttcc
     7081 gcgaagggct taacgtgctg gaatatttta tttcaacaca tggtgcccgc aaaggactgg
     7141 ctgacactgc attaaaaacg gcgaattccg gatacctgac tcgacgcctg gttgatgttt
     7201 cacaggacag tgttgtactg gaggatgatt gcggaaccct ggatggcatc gaaatgacag
     7261 ctttgataga gtccggagag ataattgaat cacttggaga ccggatttta ggacgtgttt
     7321 tgcttgatga cgtgctcgat ccaaatgttg aaaatgaaat cctgattcca gccggaactc
     7381 taatagatga gtcagatagg gatgtcattg aaaattccag gattgaaaaa gtacaaatac
     7441 gctcaccttt gacctgcatg tcagaacaag gtgtttgcag gaattgttat ggacgtgatt
     7501 tatgccacgg tggtttggtc aatttaggtg aaacagtcgg tgtgattgcc gcccaaagta
     7561 ttggagaacc gggaacccag ctaacaatgc gtacttttca tataggtgga actgcaagtc
     7621 gttcggctga acagaataaa tgggaagcag gttttagcgg agttcttcgc tttgatgacc
     7681 ttaaggaagt aaagaaccgt gaaggtaatt ttgttgttct tggacgtcgt ggggaagcag
     7741 tgattgttga aggtggcaaa aatatagctg tgtctaaact ggctgacctt gaaaacgaaa
     7801 gagagcgtga aagacgcccc ttgccgatgg gggcaactct cctgaaaaaa gaaggtgaac
     7861 tggttgaaca aggtgattta attgcagagt gggatccctt ttccattcca atcatcacag
     7921 acgtatctgg attcgttaat tttgaagaca tcaatgaggg tgaaactttc aaggaacaag
     7981 tcgatgaagt atctggtgtt tcacggaaag taattcatga atcccaaagt ttggaacaac
     8041 atccattaat tgcaatttgt gatagtaaga aaaaaacaat tatccgaaaa aatgatactg
     8101 ctacggagtt tgcgttaccg aataaatctg aattggtagt tgaaaatggg ttagaggtac
     8161 atgctggaga tgttctggca aaaatcccaa gagaactggc cagaaataag gatattaccg
     8221 gcggactgcc aagagttgca gaactttttg aagcaagagt tccaaaagat caggctatta
     8281 tcactgaaat tgacgggatt gtagaatttg acttagatgt gaagaaaaag caacgaatcc
     8341 gcatccgtcc tgaagatgga aaaggcgatt caaaagaata ccttattcca cgcggtcgtc
     8401 atatcaccgt acacatggga gagtatttgc gtgcaggtga tcctctaatt gacggttcgc
     8461 cgaatccaca tgatattctt gcagttaaag gttcaaaagc tctgcaaaaa tatttggtgg
     8521 atgaggttca acaagtttat cgacttcagg gtgttggtat caacgacaaa catattgaag
     8581 taatagtacg tcaaatgctg cgtaaggctt atgttgaaga tccgggtgat tcaagcttgc
     8641 tggttggcca ggaaattggc cgttcggaac tgaatagtat aaatcaagag ttaactgcac
     8701 agggactgcg tcccgctcgt tcaaaaccaa aattgcttgg tattacaaaa gcgtcattaa
     8761 gtacaaattc atttatttct gcagcctctt ttcaggatac aacaaaggtg ctgacagacg
     8821 cttccctggc aggaaaacac gacactttcc gcggtttgaa agaaaacgtc atcctcggcc
     8881 gacttatacc ggcaggcaca ggatttaatg tctttaataa catggactat gatcttggag
     8941 aaggagtgat tgacccggca gcaatgcgta aggcagcaga tgaagctgca agagctgcaa
     9001 tggtgaggga aatcacggaa gccgagaaaa acaaaaaacc ggcagatttg gttgccgaag
     9061 aaaaaattcc ggaagcagca taaaatactg catacaggtt catagttcag gatgcttcct
     9121 tccattgaaa cttcccactt tgagttaaaa ttgtcagttc gggactatga gctggacagt
     9181 tttggtgtga taaataatgc ggtatatcag aattatcttg aacacactcg ccatcagttt
     9241 ttggaatcac agggcctgaa attgactgct tattttgagc aaggattgag tcctgtcatc
     9301 acaaaagctg aaatagaata tcgaagctca ttacaaagta gagatgactt tgtagtgagt
     9361 ttagaattgg ctttgttaag caaagtaaag ttcatttttc ttcaggaaat ccggagcatc
     9421 cccgacaaaa cgttgattct cagtgcaagg attaccggaa ccatcctcaa ttcttccggt
     9481 cgtccttttt tacctgctgc gtttcaaaac ttgcgtactc ttcttacttc caaaacaaaa
     9541 aactgatgca acgggtgtag tgtgatttct gaaaaacaac gttccgctga ggctgcatgt
     9601 gaatacgtca aagatggcat gatagtaggc cttgggacag gttccaccgc agagtttgct
     9661 gttaaaaaaa ttggtgaact tgtccggaat ggactttcaa tacgcggcat accaacttca
     9721 gatgccacta aaatattggc agagagtgaa ggaatacctc tgattgattt ttctgagaca
     9781 atgttcattg atttaacgat tgatggtgca gacgaaattg atgccaactt aaatatgatc
     9841 aaaggcggag gtgctgcctt actccgtgaa aagattgttg cttctgcgtc aagagaagaa
     9901 atcattattg tcagtggttc aaagttggta cagcagttgg gtgcgtttcc tttgccggta
     9961 gaagttattc cattcggttg gcaagttgtt tttaatcaac tggagtcact tcagggaaat
    10021 ccagaactcc ggctcaagca gggtcaacca agcgtgactg atcaggggaa ttttatagtt
    10081 gattgtcatt ttcgaaagat tgaaaatcca gaacagctgg agcaacatct gaacatgatt
    10141 cctggagtag ttgagaacgg tctttttatc aatttatgta cacggatgat tatggcagat
    10201 ggagagaata taataataaa aaaccgcaat ttaaactgag ctgcaggact tggtctgctg
    10261 gattcccggc tagacaaatg cagcagaacg aactactata atactgagaa aaaattgttt
    10321 gtgtctataa aatataaagg agaaaaatga caattagaat tgcaataaac ggattcggac
    10381 gcattggaag aatggtggtc cgagcagcaa ataaaaatca aaatgttgaa attgtggcaa
    10441 tcaatgattt ggtaccttct gaaaatttag cctatttact gaaatatgat tcgacgcatg
    10501 gacgatttga tggtgatgtt caggcaggac aagactgttt agtggttgac ggaaaaacaa
    10561 ttgagtgtct gtctgagaga aatcctggag atttgccctg gaacaaaatg aatattgatt
    10621 atgtaattga atctacagga ttgtttacta ccagtgaaaa agcggaagag catttgaaag
    10681 ccggagcaaa aaaagtggta atttctgctc ctgccaagtc ggctgaaatc aagaccctgg
    10741 taatgggtgt caacaatgaa acatatgatc caggtacaga taatattgtt tccaatgcat
    10801 catgtaccac taactgtctg gcgccaattg taaaagttgt gctggataat tatggaattg
    10861 aagaaggttt gatgaccact gttcactctg tgacagcagc tcaaccaact gttgacggtc
    10921 catcaaaaaa agattggcgc ggtggacgtg gtggaccaca aaacatcatt cctgcttcta
    10981 ctggggcagc caaagcagta gcactatgta ttccggaaat agcaggaaaa ttaaccggaa
    11041 tgtcttttcg ggtgcctact ccgaatgttt ctgttgtgga tttgaccgtg aaactaagta
    11101 aatcgaccag ttacgaggaa atcaattcct caatgaaggc tgcttcagaa ggtaaactca
    11161 aaggtatcct gggtttcaca gatgaggatg ttgtttccag tgatttcatt ggcagtactt
    11221 attcatctat ttatgatgcc ggagccggta ttggtttgaa tgacagattt ttcaaactgg
    11281 tctcctggta tgacaatgaa atgggttact ccactcgctt gattgatctg attgaataca
    11341 tggaaggaca aaactaaatt ggttttctgc tgcaggctca aagaacagct ctttgaacct
    11401 gcggctcttc ttcaatcttt acttctcctc tctttaagcc caagccatat tctcttttaa
    11461 tctccgtaac taatttccca tgcacaaagt tgaattaaaa gaagttggtc tcgacatcgg
    11521 gttggcattc gccaggcatt tttataaaac cgactacctg cattatggat actggccgga
    11581 agcattaagt gtggatccgg caaatgtgtt agaagcgcag gaaaactatg ccaacttgtt
    11641 gctaaaacat attccggatg gagtaaattc tattttggat gtcggttgcg gttccggaaa
    11701 attttcagaa aaattgcttg acgcaggtta cacggtggat tgtgtctcac ccagtccaaa
    11761 tttaactaaa catgtgaggg agcgtctcgg agaacgagta gaaatatttg aatgccgcta
    11821 tgaagatctg aaaacaaaaa agtactatga tctgattttg tgcagcgaaa gttttcagta
    11881 cttgctcctg gaaaaggcca tggcacagac ccaggaacta ctcaatcaaa aaggccattt
    11941 gctaatttgc gattttttta aacgtgatgt tgaaggtaaa agtccagtca gtggcggtca
    12001 taaaattgag agatttctaa aatatatgca agctcagcct ttcaggcaaa tcacggacat
    12061 agacatcacg aaagaaacag ctccatcatt agatattatg cgggatttga ttcaagaagt
    12121 aattgctcca acctggaaca ttgtggccta ttatatgacc agcaactatc cccgtttcag
    12181 taaactcttt agttttgcct tccgaaaaaa aattgctcag gccaatcgta aatatctttc
    12241 aggcggcacc agcggcgaac aatttgccca ttttaaaaca tatcgtctct tcctctatca
    12301 aaaaaacagc tgaagattca aatcagagtt gataattttt tcagttctac tgcttcacaa
    12361 aatgaactct tctatttaac tttattcggt tcaatcaagc aatgtctgat ttagaaacaa
    12421 aaaatgctat tcgcatgatt ctacttacgg catctcttgg cgggatgtcg tatctgtcat
    12481 tcaataacgg cttactgctt tcctactttt cccatttggg aattcccagc gcaactatct
    12541 tgattttatt atcaatcctg ccgttaacaa tatttttagt catcattcct tttagctatt
    12601 tttcagatat atttggtaaa aaacggattg gtattatcgg gatggtgttt tcatttttcg
    12661 gctttgccct gctgatcggt gtcagttatt taccggactc attaagaata atggcagttg
    12721 gatttggaat tgttgtcttt gggataggtt cagcaatgac tttgggaaat tggtttgcac
    12781 tgcttcaccc tatcatacca gaaaatatta ggggacgttt ttttggacag ttaagactta
    12841 cttggcaatc tattggaatc ctcttcacat taatagtgat ttacattctg gaactttacc
    12901 ccaccctggg aatgtatcag gcgattctgg gtgtcattac aattctgatg ctgctaagaa
    12961 tgttttttta tcagcaaatt cctgaattgg ataaaagtac tgttaaaaaa gagtcattcc
    13021 gcataagttt tttgaaagtt cttaatattc caggatatct gccgttttgt gcatattgtt
    13081 ttttactgac tcttttcact ggtgcctgcc cacaaatttt caatttgatt gaaaaagatg
    13141 tgcttctgtg gtcaaaggtt gagattgttt ttattggaaa tttattagta gcaggagcat
    13201 tagcaggatt ctttttaggt ggcagaatga tagatcgttt tggtacaaag tatgtgtttc
    13261 tttgctgcca ttttggcttt ggaatcattc ttcttctttt tttatggcgc aatctgttta
    13321 cggaagaaat ccggattgtt gttggaattt taacgattct gtttggaatg gttcaagctg
    13381 catccagtat agcaatgacc tcagaaacac ttgccctcat tcctccgaaa aacaaatccc
    13441 ttgctaccgg actttggttt acgctttata gtggaggaac agggctttcc ggtattttga
    13501 gcggacaagc ccttgaactg ggggttttta atgaatcatg gatctggctg gggcagtcaa
    13561 tgagcagtta cgatggtttg ttgctgttgt caggaacaat ggtgttattg atgactgtca
    13621 cactgggaat gatcccatct atgataaaaa aggtgcctgc cggatggatt ccacaaagct
    13681 cgtaatctta taatcgtcag tggaatcgtc ag
//
LOCUS       DTVD01000306           13709 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_956_length_13709_cov_339.851106, whole genome
            shotgun sequence.
ACCESSION   DTVD01000306 DTVD01000000
VERSION     DTVD01000306.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13709)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_956_length_13709_cov_339.851106"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(364..1215)
                     /locus_tag="EYO68_11150"
     CDS             complement(364..1215)
                     /locus_tag="EYO68_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08400.1"
                     /translation="MFKSVNKDVWAFDAEWVPDPDAGRRLYQLPEDTPDAEVIQKMWE
                     EGGADEENPMPYLKTTICRVISIAAVVRADKGNGEVALSLTALPHDAADPGHTSEAEI
                     LSRFLNAVGEKKPQLVGFNSSKADLKILLQRAVARGIQAAKFAKRPNKPWEGYDYFDA
                     RNSEAHIDLIEILGGYGKSNPSLNEMATVCGVPGKLGVSGEEVAPMWLEGRLAEIVAY
                     NECDALSTYLIWLRMAYFGGFFNNQQYIEEQERVRNLLNTEGALPGKEHLLEFLERWE
                     MWSQLNS"
     gene            1515..2312
                     /locus_tag="EYO68_11155"
     CDS             1515..2312
                     /locus_tag="EYO68_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIC08401.1"
                     /translation="MNLSVTIIGHNEVDHLRELLPQLKWATEIVYVDCESQDDSLEFA
                     RDLGCRVFSRPNNTNLNVNKSFAVDQASGDWVFYLDPDERLPELLVMEIEETLENTVN
                     TAFRLNRRNHYFGHWLRHGSQYPDTQLRLFQRGSASFPNQHVHEKLVVEGSIGSLQND
                     MLHFPYLNISQFLSKFDFYSGVEAGYLRDAGVQVTAGNSIRFLLLKPFSRFLRRYLFK
                     GGFRDGFPGLFCAIFDALNFVVRYFKLWELTHNPPAKREQQGPDSRP"
     gene            complement(2317..3033)
                     /locus_tag="EYO68_11160"
     CDS             complement(2317..3033)
                     /locus_tag="EYO68_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HIC08402.1"
                     /translation="MKIGILQCDSTNENFRDEHGNYPEMFMSLFKSVDPDLDFKNYDV
                     QLEQYPQTPEECDAYLITGSRLSVYDDEPWIRKLEKYVVELHRQKHPLLGICFGHQMV
                     AKALGGKTEASERGWGVGVQNYQTVSTQAWIEPALEQFSLLASYKDQVTKLPEGAELI
                     AESDFCPYAAFRIDDHILTFQGHPEFQKAYSKALLNLRKEILGPKVFEAGKKSLEQTI
                     QPQQITHWMLNFLRKGDPHK"
     gene            3304..4233
                     /locus_tag="EYO68_11165"
     CDS             3304..4233
                     /locus_tag="EYO68_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIC08403.1"
                     /translation="MEKNKTAQWVDISKEVQDALKTDKAVVALESTIISHGMPYPQNL
                     ETALAVENIIRNEGAFPATIAVSGGRIKIGLSRQELQQFAQNSDPVKVSRRDLPVVLS
                     QKLSGGTTVAATMICAQIAGIPVFVTGGIGGVHRENEKTMDVSADLMELAHTNVAVVC
                     AGIKSILDIPRTLEYLETHGVPVIGYQTAEFPSFYTPSSGSMVQTRIDTPEDIARCMK
                     IKWDLGLAGGLVIGNPVPPKDAMDESLIEGAITEALKEATEKEIEGKEVTPYLLERIN
                     QLTHGESLKSNIALVRNNASLGAKIALAYKALL"
     gene            complement(4215..6293)
                     /locus_tag="EYO68_11170"
     CDS             complement(4215..6293)
                     /locus_tag="EYO68_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIC08404.1"
                     /translation="MSQLILVLEENPEIQSLIAASLKASAISVTQESNPDLFVQQALN
                     LEPDLILLSNSGGDQNYKNCREIRKDPQLKNIPIILLVNPKDELDEKIISELGIDGLL
                     RKPFEASMLQEQLSQFVTLDDNFGIEPDNEGEDFKIDMSSIDNQLNDIKEGEQEVNSP
                     VEEGSQVTEELEQNALEPKFTSSAGSEMDSMILDDISADMDISKDTVKKAEVELDENE
                     ITSNGLEVINDEEMTMDNGFEFDLKLDEDGIEIDSEEVVTDTVKTETDPASTGLAQLK
                     DSGLEDKYAESRLKVEGEKTEQDLKAPADIDLEVNDFEDLDDIWSRSPELDQTPREGL
                     TDISLEEEDFQPEFPENLSSFEEPAEPSIQDTDSDSRITSESVQELDNYELEPDFEET
                     GEESLSSLDNKLLTDSVEQDLEETVEIEDAEQFVEDTSADEFEEDFDESQGDADIMSM
                     VHESIGEQEESAESAMIELEDLSEQMEALDSAETEFEIEEEQFEEDLEKEKLEVEELS
                     EDELAVSEDDSEDELVEFMLDELGDLLETDEEGSVDGEADYGDPLVEELEESLAAESK
                     DLQDVDAEFDKKVYTEISLGLGLEEDVFDSWDDAEDAFMGFDREKEITEEAPDQTETE
                     ASAFESFDDEEEKFEKADSFSFTENELKEIVTNSVQNALEKSIAASLVELAVSELKTQ
                     VIRMDQSRAL"
     gene            6502..7557
                     /locus_tag="EYO68_11175"
     CDS             6502..7557
                     /locus_tag="EYO68_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgP/YjgQ family permease"
                     /protein_id="HIC08405.1"
                     /translation="MPFKIISKYIASEFNRYFMLFLGAFVLMSLVGNFFGNLADVFTD
                     WQSFLKFLQETALLLPLLLELIIPITVLLATIATYSSLNKNAELLAIRSSGIGGWRLA
                     FPVLAVSALIASFAYFSQNYMYTWMHQTWVKQENSARLPPLWKVGEEQSIYYFGNRQH
                     NGRISSITSFNWKQIPFQLLGRTAIERGEQQNNSWIFHDIQKYIFQDENLRLEHVEQW
                     RIETEKLPTVPFEIPISPHHQPLLDLYENTLKLQSEGQDVTRHWVAIFQKTAYPFQIF
                     IMVLIGFGLSASYNRRGMAAESLAISCLLGILFWILNQITMAVGGAGLIFPFFAAWSG
                     NIIFLALALWLLTYNRL"
     gene            complement(7605..8876)
                     /gene="ftsY"
                     /locus_tag="EYO68_11180"
     CDS             complement(7605..8876)
                     /gene="ftsY"
                     /locus_tag="EYO68_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIC08406.1"
                     /translation="MTTLILIGFAIFIILLCLFVWYRQHSRSLKSKAPEELSGRKKEK
                     RLVQLEKEHAKTLELQIKEEEKLREEKESAKLAKAEQREKELQEKIASIEEERLNQQV
                     LQREIEKTAETVETPDEVDSFLERLRKGVIKTRTQLQDNLAEAVLGRKEINEDLLDDL
                     EEVLIGSDIGPETTQRILDAITEKVEREELSSPQVLQSEIQLEIQKIMSKTYAVLGTA
                     ERKPLILLFVGVNGVGKTTTIGKIAAQYRQQGKKVLMGAGDTFRAAAIEQLVEWSRRA
                     DCDIIQKEPGSDPSAVMYETVQKGIDEDYDVVICDTAGRLHTKKNLMEELKKMVRVIR
                     KLIPDAPHEILLVLDATTGQNAIFQTREFMEATDLTGLIITKLDGTSKGGVVIGIVNE
                     FDIPVRYIGVGEQVEDLRPFDAQQFTESIFA"
     gene            9077..11098
                     /locus_tag="EYO68_11185"
     CDS             9077..11098
                     /locus_tag="EYO68_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIC08407.1"
                     /translation="MSTWSIASLNPVQRKIVEHKTGPALVIAGAGSGKTRVITHRVAQ
                     LIHSGIPASSILLLTFTNKAANEMAQRVGRALKNSTEQQKIIHGTFHSVGSRFLRRNA
                     KLLNYYNNFSILDSSDSRDLIKASLAETVGKPGKYFPKAAVLQNMFSLAFNRFCTQKM
                     ILDQPYHERSFHLEQLISADFPNFEENTETILNILAAYRHKKKRGQVMDFDDLLENWL
                     ELLLRHGEGLPLCRQIQYILVDEYQDTNQVQANILYRLSRPHKNLMVVGDDAQSIYSW
                     RGADFGNMLEFPERYQAVTYNMEENYRSSPEILDAANRSINYNTRQFEKNLFSSLAEG
                     EKPLVHYVWASQDEAELVLENILSFRDKDISLNEMSVLYRNHVQSAALQVVLTHAGIP
                     FVVHSGVKFFEQAHIKDVTAFLKVLFNPLDEIAWMRLLRLLPGIGNTTAHRIFRVFQS
                     QQAVRLTKDNVELQKLIPKKALESWNVLLEIFQQMLEESVTPSHMIGLLYKSYYREVL
                     FSSFENAPQRETDIRYLEEFAGNYNKLEAFLNELSLVGSSIITDHEAEQQDNQDTLTL
                     TTIHQAKGLEWKVVFIIGLTEGLFPHQRSLGTEEQIEEERRLFYVAMTRAKRHLLITA
                     PVVSSGYSGGISKRSRFINELAEETINTEIHTRDEYSYGNRGAQTQFYF"
     gene            complement(11104..11622)
                     /locus_tag="EYO68_11190"
     CDS             complement(11104..11622)
                     /locus_tag="EYO68_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIC08408.1"
                     /translation="MEKLKNATLGGGCFWCLEAVYSKLEGVKSVAPGYAGGTKLNPTY
                     EQVCTGNTGHAEVIQIDFDPQKTSYETLLDWFWRCHDPTTLNRQGGDIGTQYRSVIFY
                     ENEAQKTTANESKKDAEKSGMFDAPIVTEIATLPEFYPAEDYHQDYYSNNSNAGYCTY
                     VIRPKLAKLNLE"
     gene            complement(11610..12401)
                     /gene="recO"
                     /locus_tag="EYO68_11195"
                     /pseudo
     CDS             complement(11610..12401)
                     /gene="recO"
                     /locus_tag="EYO68_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
     gene            complement(12409..13668)
                     /locus_tag="EYO68_11200"
     CDS             complement(12409..13668)
                     /locus_tag="EYO68_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08409.1"
                     /translation="MKRLILIAVVLLLLGSMGYFATQNSHNVSLNFFGNFSIQLSVWM
                     VIAGSFVAGWAVTEIWQFISHPQRFVQSFLGKFSQYKDNKKQQITQNFENASLLRDPK
                     QVRKSYNKLLNQETSLSIRVQYIEQLRYEKSAEELLKKYAELRTKFQGNLQVLLPYLK
                     LACEVSEWDLAERLSHEILRITPDHPDALEGLRQFYITRQDWVGCIGQERELLKKFSG
                     SLITKNISMTHEDHLQKALRQDPKCLSNWSFRYLPQKRDKKNDKPLEAIGEAAQLQKS
                     GMFLEAARVLKEAFERTAFPELLELLEEVYRESGTDEQILEMVGKLHNSRQRSVPSSL
                     LFAKLLYQCDRLDEVSKVLDKVKLPAGSTKFPMAILGSEDKGQQMTEVWSDLYHALRY
                     LIAVRQERSEDALQEAKPLLREAKILE"
BASE COUNT         3851 a         3065 c         2599 g         4194 t
ORIGIN      
        1 gccgggtcaa aaatttcatt ttaacaatat atagagtttt tgtacaataa aaaaaataaa
       61 ggtgataaaa ttctgaaata cctgacaaac acgcgtgtca tcagttcttt gtcacaacta
      121 ccacatctgt ggtgttttaa ataattcggt atgtcaaaaa atgaatctca tgagtcttca
      181 attctcgtaa gatactgata ttatttactg cggtaagtca tatcgaccga agggagagac
      241 atgataaagt gccgaaaacg tataagaccc ccctctcatt cggtcgggat gacacgataa
      301 aactcgtaac tgtcacgaaa gaatctatga ctgtaaagaa ttaaccgaag cggcttacat
      361 tgttcaggaa tttagttgag accacatttc ccagcgttcc agaaattcaa gcaaatgctc
      421 tttgcccgga agggcccctt ctgtattgag caaattccga acacgctcct gttcttcaat
      481 atattgctgg ttattaaaaa aacctccaaa ataagccatc cgcaaccaaa tcaaatatgt
      541 acttaaagca tcacattcgt tataggctac aatttccgcc agacgcccct ccagccacat
      601 tggcgctacc tcttcaccac ttacccccag ttttcccgga acaccacaaa ctgtcgccat
      661 ctcattcagt gaaggattag actttccgta acctcccagg atttcgatca aatcaatatg
      721 agcttcactg ttacgtgcat caaaataatc ataaccttcc catggtttat tggggcgttt
      781 tgcaaatttt gctgcttgaa tacctcttgc gacagctcgc tgcagaagga ttttaaggtc
      841 tgcttttgat gagttgaatc ccaccagttg aggttttttt tcgcccaccg cattaaggaa
      901 acgtgacaaa atttctgcct ctgatgtgtg tcccggatcg gctgcatcat gaggcaaagc
      961 cgtcaaagac aaggccacct ctccatttcc tttatctgca cgaacgaccg cggcaatgga
     1021 aattacgcgg caaattgtgg ttttgaggta aggcatcgga ttttcttcat ccgcaccgcc
     1081 ttcttcccac attttttgga ttacttctgc atcaggagta tcttccggaa gctgatataa
     1141 tcttcttccc gcatccggat ccggcaccca ttccgcgtca aaagcccata catctttgtt
     1201 gactgatttg aacataagac ctcccttgta cttctgtaga ttgattcccg aagtttagcg
     1261 ttttgaaaac gtattggcaa tctaatctat gacagaacaa tgttttgtgc aagaaatgca
     1321 aggagaaaaa ccgctgcctg agtaatcact cgattccaat tttacagaaa aaataatcgt
     1381 cagagaacaa tcagaaacgt ctgcaacatt gacaagatct taaacttgtc atagattggt
     1441 caacagatca ttttcaaatc aagctttcaa tattttttct ggcagcaagc aaactgaagc
     1501 aaaaaccaaa ctccatgaat ctcagcgtta ccatcatcgg tcataatgaa gttgatcatt
     1561 tacgggaatt attgccccaa ctcaaatggg caactgaaat cgtctatgtt gactgtgaaa
     1621 gtcaggacga ctcactggaa tttgcacgtg atctaggctg ccgtgttttt tcacgaccaa
     1681 acaataccaa cctgaatgtt aacaagagct ttgcggtgga tcaagcttcc ggggactggg
     1741 ttttttatct ggatccagat gagaggctgc ctgaactgtt ggtgatggaa attgaagaga
     1801 cgcttgaaaa tacagtcaac acagcattcc gcttaaaccg ccgtaatcat tactttggac
     1861 actggctgcg tcacggaagt caatatccgg acacacagtt acgtttattc cagagaggtt
     1921 ctgccagctt ccctaaccaa catgtacatg aaaaactggt agtggaggga agtatcggca
     1981 gtctgcaaaa tgatatgctc cactttcctt acctgaacat cagccagttt ttgagcaagt
     2041 tcgattttta ttccggagtt gaggcaggtt atctccggga tgccggcgta caggtgactg
     2101 caggaaattc tattcgcttt ctgctactca aaccattttc ccgtttctta agacgctatc
     2161 tgtttaaagg aggattccgc gacggatttc cgggattgtt ctgtgcaatt tttgatgccc
     2221 tgaattttgt cgtccgttat ttcaagctct gggaactaac tcataatcct ccagcaaaaa
     2281 gggaacagca aggtccggat tcgagaccgt aaccggttat ttgtgaggat cacctttcct
     2341 gaggaaattc agcatccagt gtgttatttg ctgaggctgg atggtttgct ccagcgattt
     2401 ctttcctgct tcaaatactt ttggacccaa tatttctttc ctgagattca gtaatgcttt
     2461 ggagtatgct ttctgaaatt ccggatgtcc ctgaaatgtc aaaatatgat catcaattct
     2521 gaaagcagca taagggcaaa aatcactttc cgcaatcaat tcggcacctt ccggaagttt
     2581 tgtaacctga tccttgtaac ttgccaaaag tgaaaattgt tccaaagcgg gttcaatcca
     2641 ggcttgtgta gataccgttt ggtagttttg cactcctaca ccccaacctc gttctgatgc
     2701 ttcagttttt ccgcctaaag ctttggcaac catttggtgt ccaaagcaaa tgccaagcag
     2761 aggatgtttc tgcctgtgca gttcaactac atacttttcc aattttcgaa tccacggttc
     2821 atcatcataa acgcttaaac ggctgccggt gatcaaatac gcgtcacact cttccggagt
     2881 ttggggatac tgctccagct gtacatcata atttttaaaa tctaaatccg gatcaacaga
     2941 tttaaaaagt gacataaaca tttccggata gttgccgtgt tcgtcccgga aattttcatt
     3001 ggtggaatca cactgaagaa taccaatttt catatagtcc ctcctgaaaa aagaaatagc
     3061 ctaaacaggc tagcagaatt gacaatattt gcaatcagat tgataaatga gaatccccct
     3121 ttttcatttt cagataaaca attatcagta accatcatga ttattttatc ttgaatccgt
     3181 tgcagacagt ccaatcatta aatccggctc ttctcttacg atgatgattg acagctacag
     3241 ccgtttccgt tagtttcaga agtaacaaat aaaatcataa aacattttat attcccaaat
     3301 tatatggaaa aaaataagac agcacagtgg gtggacattt ccaaggaagt tcaagatgca
     3361 ctgaaaacag ataaagcggt tgttgcactg gaatcgacaa ttatttcaca tggaatgcct
     3421 tatcctcaaa acttggaaac agcactcgca gttgaaaata ttattcgaaa tgagggggct
     3481 ttccccgcca caattgctgt ttcaggaggc aggatcaaga tcggtttgag caggcaggag
     3541 cttcagcagt ttgctcaaaa cagtgatccg gtaaaagtca gccgccggga tttaccggta
     3601 gttctttcac aaaaactgag cggtggcacc actgtagctg caactatgat ttgcgcacag
     3661 attgccggaa ttccagtctt tgtgacagga ggcattggcg gagtacaccg cgaaaatgaa
     3721 aaaaccatgg acgtttccgc agacttgatg gagttggctc atacaaatgt tgctgtagtc
     3781 tgtgccggaa taaaatccat tcttgatatt ccccgaactc ttgaatattt ggaaacacac
     3841 ggtgtgcctg taattggtta ccagacggca gagtttcctt cattttatac gccgtccagc
     3901 ggttcaatgg ttcaaacccg aattgacaca cctgaagaca ttgcacgttg tatgaaaatt
     3961 aaatgggact tggggttggc cggaggtctt gtgattggga atcccgtgcc tccgaaagat
     4021 gcaatggatg agtcactaat tgaaggagca attacagaag cgctgaaaga agcaactgaa
     4081 aaggaaatcg aaggaaaaga ggtgactcca tatttgctgg agcgaatcaa tcagcttact
     4141 catggagaaa gcctgaagag taacattgca ctggtacgta ataatgccag cttaggagcc
     4201 aaaatcgccc tggcttacaa ggctctgctt tgatccattc gtattacttg tgtttttaat
     4261 tctgaaactg ccaattcgac aagagatgct gcaatagatt tttccaaagc attctgaact
     4321 gaatttgtga caatctcctt caactcattt tctgtaaaac tgaaactgtc cgctttttca
     4381 aatttttctt cttcatcatc aaaactttcg aaagcacttg cctctgtttc tgtctgatcc
     4441 ggagcttctt cagtaatttc tttttctcga tcaaatccca taaaggcatc ctcagcatca
     4501 tcccaactgt caaacacatc ttcttcaagt ccaagtccaa gtgaaatttc agtatacact
     4561 tttttgtcaa actctgcatc aacatcttgc aagtctttgg attctgctgc gaggctttct
     4621 tccagttcct caacaagcgg atctccataa tcagcttctc cgtccactga tccttcctca
     4681 tccgtttcca gaagatctcc cagttcatcc agcatgaatt ctaccagttc gtcttctgaa
     4741 tcatcttctg acactgctaa ttcgtcttca gataattctt caacttccag tttttctttt
     4801 tccagatcct cctcaaactg ctcttcttct atttcaaact ctgtttctgc agaatcaaga
     4861 gcttccattt gttcactgag gtcttccagt tcaatcatag cactttctgc tgattcttcc
     4921 tgctcgccaa tactttcatg aaccattgac ataatatccg catctccctg actctcatca
     4981 aaatcttctt caaattcgtc tgcagatgta tcttctacga attgttcagc atcctcgatt
     5041 tcaacggtct cctcaagatc ctgctccaca gaatccgtta acaatttatt gtcaagtgaa
     5101 cttagcgatt cctctcctgt ttcttcgaaa tcaggttcca gttcataatt atccaattcc
     5161 tgaactgatt ctgacgtgat cctgctgtcg gaatcagtgt cttgaatcga aggttcggct
     5221 ggctcttcaa atgaggagag attttctgga aattcgggtt gaaaatcttc ttcttccaaa
     5281 cttatatccg tcaggccctc tctaggtgtc tgatccaatt ccggagagcg tgaccagata
     5341 tcatccagat cctcaaaatc atttacttca agatcaatat ccgctggtgc cttcagatcc
     5401 tgttctgttt tttcaccctc aaccttgagg cggctttcag catatttgtc ttccaaaccg
     5461 gagtccttaa gttgcgccaa tcctgttgag gcaggatctg tctcagtttt tacagtgtct
     5521 gtaacaactt cttcggagtc aatttcgatt ccatcttcat caagcttaag atcaaattca
     5581 aaaccgttgt ccattgtcat ttcttcatca ttgatgactt caagaccgtt agatgttatt
     5641 tcattttcat ccagctcaac ttctgccttt ttcactgtat ccttagaaat gtccatatct
     5701 gcagaaatgt catccagaat cattgaatcc atttcactgc ctgctgaaga ggtaaatttt
     5761 ggttccaggg cattctgctc taattcttct gttacttgag atccttcctc aactggtgag
     5821 tttacttctt gttccccctc tttaatatca ttcaattgat tgtcaattga gctcatgtct
     5881 atcttgaaat cttcaccctc attatccggt tcaatgccaa aattgtcatc cagagtaaca
     5941 aattgactta gctgctcctg aagcatcgat gcttcaaagg gtttgcgaag caacccatca
     6001 attccaagct ctgaaataat tttttcgtca agctcgtctt ttgggtttac aagtaaaatg
     6061 attgggatat ttttcagctg cggatctttg cgtatttctc tgcaattctt gtagttttga
     6121 tcgccgcctg aattactgag taaaattaaa tccggctcca gattaagtgc ctgctgcaca
     6181 aataaatctg gattagattc ttgagtaact gaaattgcac tagccttcaa cgaggcggca
     6241 attaaacttt gaatttctgg attttcttcg agtacaagaa ttaattggga catgaatatt
     6301 gctttttagc tcctaaattg tcagaacaaa gttattaaaa aaaattctct taaaactaat
     6361 cctgtagctt atatgcaaga taatgcccta actattgaac aaatattaac tagatttgtt
     6421 atttactttg aaccttttta taagaaactt tatcctgttc tgaatactat tttgcttatt
     6481 caccaatgag gaccggaaac tatgccgttc aaaataatct caaaatatat tgcatcagaa
     6541 ttcaaccgtt attttatgtt gtttctcggt gcatttgtat tgatgagtct ggtagggaat
     6601 ttttttggta accttgcaga tgtttttact gactggcaga gttttttgaa gttccttcaa
     6661 gaaacggctc ttttgctgcc tctattgctg gaactaataa taccaattac ggttttactg
     6721 gcaactattg ccacatacag ttcactgaac aagaatgctg aattgctggc aatacgcagt
     6781 tcgggaatcg gtggttggag actggctttt cctgtgttag ctgtttctgc tttgattgcg
     6841 tcttttgcgt atttcagcca gaattacatg tacacatgga tgcatcaaac ttgggttaaa
     6901 caggaaaatt ctgcacgctt gcccccactc tggaaagtgg gtgaagaaca aagcatttac
     6961 tattttggga atcgccaaca taatggccgc ataagttcta tcacctcatt taactggaag
     7021 caaattcctt tccaacttct gggaagaact gcaattgaac ggggagaaca acaaaacaat
     7081 tcatggatct ttcacgatat tcaaaagtac attttccagg atgaaaattt gcggcttgaa
     7141 cacgttgaac aatggagaat cgaaacagaa aagttgccca ctgtaccctt tgaaattccc
     7201 atttctccgc atcatcaacc cctccttgac ttgtatgaaa atactcttaa acttcaaagt
     7261 gaaggacaag atgtaactcg tcattgggtt gcgattttcc agaaaaccgc ctatccgttt
     7321 caaattttta tcatggttct gattggtttt gggctgtctg catcatacaa cagacggggg
     7381 atggcggcag aatctctggc aatcagttgc ttactcggga ttttgttctg gatactcaat
     7441 cagataacca tggcagtcgg cggtgcaggc ctgatattcc ctttctttgc agcatggagc
     7501 ggaaatataa tttttctcgc tctagccctc tggctgctga cttataaccg gctataatct
     7561 attttgcggt gttgaattta cgatttatat ccaaaatcaa aaagttaagc aaaaattgac
     7621 tcagtgaatt gctgggcatc aaagggtcgt aaatcttcaa cctgttcacc tacaccgatg
     7681 taacgcacag gaatatcaaa ctcgttgacg atgccgataa caactcctcc cttggaagtg
     7741 ccatccagtt tagtaattat aagtcctgtt aaatctgtag cttccatgaa ttcccgagtc
     7801 tggaatattg cattttgacc tgtggtagca tcaagtacca gcaggatttc gtggggtgca
     7861 tcaggaatca acttgcgaat cacccgcacc atttttttta attcttccat caaattcttt
     7921 ttggtgtgca aacgtcctgc tgtatcacag atcactacat cgtaatcctc atctattcct
     7981 ttttgcaccg tttcatacat caccgcagac ggatcacttc cgggttcctt ttggatgatg
     8041 tcgcaatcag cccgcctgct ccactccacc aactgttcaa tagctgctgc ccgaaaagta
     8101 tcacctgcac ccatcaaaac ttttttaccc tgctgacgat actgtgcagc aatttttccg
     8161 atagtagttg tttttccaac accgtttaca ccaacaaaaa gcaaaatcaa cggcttccgt
     8221 tccgcagtcc cgaggacggc atatgtttta ctcataattt tttgaatttc cagctgaatt
     8281 tctgactgca aaacttgtgg actgcttaat tcttcacgct ccaccttctc tgtgattgca
     8341 tcaagaatac gctgggtagt ttccggacct atgtctgaac cgatgagcac ttcttccaaa
     8401 tcatccagta agtcttcatt tatttctttt ctacctaaaa ctgcttcggc taaattatct
     8461 tgtaactgtg ttcttgtttt aatgacgcct ttacgtagac gctcaaggaa agaatctacc
     8521 tcatcaggag tttccacggt ttcagcagtt ttttcaattt cacgctgaag aacctgctga
     8581 ttcagacgtt cctcttcaat tgaagcaatt ttttcttgaa gttctttttc ccgttgttcc
     8641 gcttttgcaa gttttgcgga ctctttttct tcccgcagtt tttcttcttc cttgatctgc
     8701 aactcaagtg ttttggcatg ttctttttcc aactgcacca ggcgcttctc ttttttcctc
     8761 ccggaaagtt cttcgggagc tttacttttc agtgatctgc tatgctgccg ataccataca
     8821 aaaagacaca aaagaatgat aaaaattgca aaccctatca aaatcaaggt cgtcatatcc
     8881 tctgctatcg gcactccttt gttaaagatt ctactcaaaa acaggtttac tgcttcacgg
     8941 aaagtcgaaa cgatttgttt tatttcttca aaaaacatgg aaattatttt gctagttgtt
     9001 tttgttttag actaaaattt ggcattatag ttagaaaata tcaacggaaa gacatctaaa
     9061 atcaccagtc cagtatatgt ccacctggtc aatagcatct ttaaatccgg tacagcggaa
     9121 aatcgtcgaa cacaaaacag gccccgcttt agtcattgcc ggagcgggca gtggaaaaac
     9181 cagggtgatt actcaccgtg tagcacagct gattcattcc ggaattcctg cgtcttccat
     9241 tcttttgctg acattcacca acaaagccgc caacgagatg gcgcaaagag taggccgggc
     9301 tttaaagaat tctacagaac agcaaaagat tattcatggg accttccaca gtgtgggaag
     9361 ccgattcctc agacgaaatg ctaaattact aaactattac aataatttca gcattctgga
     9421 ttcctcagat tccagggacc tgataaaagc ctcacttgca gaaacagtag ggaaaccagg
     9481 caaatatttt ccaaaagctg ctgttctgca aaacatgttt tcattggcct tcaatcgttt
     9541 ttgtactcaa aaaatgattt tggaccagcc ttaccatgaa cgcagtttcc acttagagca
     9601 gctgatttct gcagatttcc ccaattttga agaaaacact gaaacaatac taaacattct
     9661 agctgcctac cgtcacaaga aaaaacgcgg acaagtgatg gactttgatg atctgctgga
     9721 aaactggctg gaactactct tgcggcatgg tgaaggactc ccgctttgcc ggcaaattca
     9781 atatatcctg gtagacgaat atcaggatac caatcaggtt caagccaata tcttataccg
     9841 gctctcaagg ccgcacaaaa atttaatggt tgtcggcgac gatgctcaat caatatacag
     9901 ctggcgcggc gctgattttg gcaacatgct tgagtttccg gaacgctacc aggcagtcac
     9961 ctacaacatg gaggaaaatt atcgcagttc tccagaaatt ttagatgctg ccaaccgaag
    10021 tattaattat aacacacgtc agtttgaaaa gaaccttttt agttctttag ctgaaggaga
    10081 aaaacccctc gtacattatg tttgggcttc tcaggatgaa gctgaactgg tactggagaa
    10141 tatactcagt ttccgggata aagatatttc tttgaacgaa atgagtgtgc tttaccgaaa
    10201 tcatgtacag tctgcagcac ttcaggtagt gctgacacat gcaggtattc cgtttgttgt
    10261 tcattcagga gttaaatttt ttgaacaagc tcatatcaaa gatgttacgg cttttcttaa
    10321 agtacttttt aatcctcttg atgaaatcgc gtggatgcgt ctgcttaggc tccttcccgg
    10381 gattggcaac actactgcac accggatttt cagggtattc cagagtcaac aggcagtccg
    10441 tcttactaaa gacaatgttg aactgcaaaa actgattcct aaaaaggccc ttgagagctg
    10501 gaatgttctg ctggaaattt ttcagcaaat gctggaggaa agcgtaactc cttctcacat
    10561 gataggatta ctgtacaaga gctattaccg cgaggtgctt ttcagcagtt ttgaaaatgc
    10621 ccctcagcgt gaaacagata ttcgttatct tgaagaattt gctggcaatt ataataaatt
    10681 ggaagcgttt ttaaatgagt tatctttggt tggttcgagc attatcactg atcatgaagc
    10741 cgaacaacag gataatcagg acactctcac tctgaccacc atccatcagg ccaaaggtct
    10801 ggaatggaag gtggtattca taattggact tacagaagga ttgtttcctc accaacgaag
    10861 tttgggaaca gaagagcaaa ttgaagaaga gcggcgcctg ttttatgttg caatgacacg
    10921 tgcaaaacga cacctgctca ttactgcacc ggtggtttcg tcaggttatt ccggaggaat
    10981 cagtaagcgc tcccgtttta tcaacgaact cgcagaagag acaatcaaca ccgaaattca
    11041 tacccgtgat gagtattctt atggaaaccg gggggctcaa actcaatttt atttttaata
    11101 aaactattcc aagtttaact tcgcgagttt aggacgaatt acgtaggtac aataaccggc
    11161 atttgagtta ttgctgtaat aatcttggtg gtaatcttct gcaggataga attccggcaa
    11221 tgttgcaatt tcagtaacaa ttggagcatc aaacattcca gatttttcag catctttttt
    11281 tgactcgttt gccgtagttt tttgtgcctc attttcatag aaaataacgg aacggtactg
    11341 cgtcccaatg tctcctccct gacggttaag agttgtagga tcgtgacaac gccaaaacca
    11401 gtcaagcaaa gtttcgtagc tcgttttctg tggatcaaaa tcaatttgaa tcacctctgc
    11461 atgtcctgtg tttccggtac atacctgttc ataagttgga tttagttttg tacctcctgc
    11521 atagcccgga gcaacagatt tgactccttc cagtttggag tagactgctt ccaaacacca
    11581 aaaacagcca cctcccagag ttgcgttttt taatttttcc atgaattctc cttcaaaagt
    11641 gcgtgagact tcagatttcg gccaaaaaaa tgcctgaagt atataaagag tgcatgatcc
    11701 agttcctcta aattattttg ggttgggcga aaaacagaat cactttgttt tgcattttgc
    11761 cgtacatgca aaaaggctaa gcttcctgga tgcaaggcag ccgagtaaga gctaggaata
    11821 cagcatcttg gacagcgaac acctcccaag cttgcgtcca actggtacgg aactgaaaat
    11881 tttggagcag gatttcgtcc ggatctttcc tgccaaagtt cgccaccgca ctgaaaacaa
    11941 atttccaaat ttggtgtgat tccaagtaat tttagaagcc gtatttcaaa atttaatttt
    12001 atttcagtgt cagtttgaga ctctgagagc agagacattg cattcttcag taaatcaaaa
    12061 tattcatgag aatctaattc aggaatttca cattgaagca gcaattccga aaagtaactg
    12121 gcgtgtagaa actttgaata atttttacgg acaagaggaa agctttcctg caattcgcat
    12181 ttccggattc taaccaaatc catgttcggt ttttcagaaa actcaaaatg gttaagaaca
    12241 aacaattctg cttttgcagc atttcccgac ctcaaacttt ttccaccgtg aagaattcct
    12301 gctttcttac ccgccttggt cgtcaggaaa gtcagaatgc aatccttttc cttaaaatcc
    12361 attgttttga gaacaattcc atctgtctca attactttca tacttaaatt attccagtat
    12421 tttagcttcc ctcagcaaag gctttgcctc ctgcagtgca tcttcagaac gttcctggcg
    12481 gactgcaatt aaatagcgga gtgcatggta aagatcactc caaacttcag tcatctgctg
    12541 tcctttgtct tctgatccaa gaattgccat agggaacttt gttgaacctg caggaagttt
    12601 cactttgtct aaaactttcg atacttcatc cagccgatca cattggtata gcagcttcgc
    12661 aaaaagaagt gaagatggaa ctgaacgctg acgggaattg tgcagtttgc cgaccatttc
    12721 caaaatttgt tcatcagttc cactttcccg ataaacctcc tctaataact ccagcaattc
    12781 tggaaaagcg gtccgctcaa aagcttcttt aaggactcgt gcagcttcca aaaacatacc
    12841 tgatttttgg agttgtgctg cttcaccgat agcttccaaa ggcttatcat tttttttatc
    12901 cctcttttga ggaaggtagc ggaaagacca gttactcaaa cactttgggt cttgacggag
    12961 tgctttttgt aaatgatctt cgtgtgtcat tgaaatattt tttgttatca ggctgccgct
    13021 gaattttttc agcaattccc tctcctgacc aatacagcca acccagtcct gtcttgtaat
    13081 ataaaattgc cttagtcctt ccaatgcatc cggatgatca ggagttatcc ggaggatttc
    13141 atgacttaac ctctctgcca gatcccattc actgacctca caggcaagtt tcaaatatgg
    13201 cagtaatacc tgcaaattac cctgaaattt ggtacgcagt tcagcatatt ttttgaggag
    13261 ttcttctgcg cttttttcgt aacgcaattg ttcaatgtac tgtactctga tcgacaacga
    13321 agtttcttga ttgagtaatt tattatagct tttcctcacc tgttttggat cccgcagcaa
    13381 agatgcattc tcaaaatttt gtgttatctg ttgctttttg ttgtctttat actgggaaaa
    13441 tttaccaaga aaactctgta caaaacgctg aggatgtgaa ataaactgcc aaatctcagt
    13501 caccgcccat ccagcgacaa aggacccggc aatcaccatc cacactgata attggattga
    13561 aaagtttccg aaaaagttca gggaaacatt gtggctgttt tgtgttgcaa aataacccat
    13621 actgcccaaa agcaataata cgactgcaat taaaattaag cgtttcatag tttcgtttta
    13681 aaatgtgaaa aacataaatt aagtttaat
//
LOCUS       DTVD01000307           13674 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_963_length_13674_cov_403.328145, whole genome
            shotgun sequence.
ACCESSION   DTVD01000307 DTVD01000000
VERSION     DTVD01000307.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13674)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_13674_cov_403.328145"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="EYO68_11205"
     CDS             complement(<1..341)
                     /locus_tag="EYO68_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIC08410.1"
                     /translation="MPEKNKQIKILLLIAVFTIVVALFFVFDLQKYLTLEYLKSSKAF
                     FISSYEKNPILVLGSYFLFYIVITAFSLPGAVWMTLGGGAFFGLFAGTVIVSFASSIG
                     ATLAMLIARFLL"
     gene            complement(411..647)
                     /locus_tag="EYO68_11210"
     CDS             complement(411..647)
                     /locus_tag="EYO68_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08411.1"
                     /translation="MMKQTANPDFFQLAFGDGTPKKALMTALVVGTILATINHGDVIL
                     KGESINYFKIMLTYCVPYCVTTWGAIHGKRVKLL"
     gene            complement(931..1182)
                     /locus_tag="EYO68_11215"
     CDS             complement(931..1182)
                     /locus_tag="EYO68_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08412.1"
                     /translation="MQLIFDGGGTKWIEEFSKEHKMTPLSQSLKSSGVIAGVCDYCDT
                     SFGGEKDLLKKEELPLFDEYKGHPSIARLFADGYQTITL"
     gene            complement(1184..2236)
                     /gene="rlmN"
                     /locus_tag="EYO68_11220"
     CDS             complement(1184..2236)
                     /gene="rlmN"
                     /locus_tag="EYO68_11220"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIC08413.1"
                     /translation="MSKLYNIKNWTRHNLRAWMAEKSIKHSKVQEFRADQIFYWLYQK
                     KAESFSEMHNIGRETRKIMEENFWISSLEKAETQQSQDGSIKYRLLLDDGLSVESVFM
                     PHTSHNTVCVSSQVGCGMGCDFCMTGTMGLVRNLKTAEIIDQVLTVWNDQPEGKNIRN
                     IVFMGMGEPFHNYQNLMQALEILTDEHGFNFSQRRITVSTSGLLPKIRRFGKEKIKAN
                     LAISLNGVTDDVRSKLMPVNIPYNLEKLIKVCREFPLESRRRITFEYILIRDLTDSIK
                     DAKGLISLLHGMKFKINLIPFNSAPGSKYQSPSVEQSRNFQKYLLDHGVVAPIRISKG
                     QDIQGACGQLVVGIKA"
     gene            2361..3542
                     /locus_tag="EYO68_11225"
     CDS             2361..3542
                     /locus_tag="EYO68_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08414.1"
                     /translation="MKCLKCGWSNSDTAAYCTKCHAYLSEQPGMESIDAIKALILSLF
                     FTGIFYLVFPIPVIRSEYWLQLFSGHISETIVALVLWSLFLIFFKWRQFRQQLRAYEA
                     FRNQIMHDSLSKGIYVRDVDERILEISQFMQEQKVKKFQDSVIFRRVRRTLRHLKAIP
                     KKEEITSILNYQADIDHNRMQSGYALLNVFIWAIPILGFIGTVFGIGQSVGEFSDFIR
                     STGGTDLNTQMRSALGGVTSGLSVAFSTTFIALVGVVPIMMLASSLRKREEDLLLSVE
                     DYCLEDLLPNLHVRPGDEMLDDTVSEHLEQMAEFSENWRKQVSPVLESVERHSKGFAA
                     QVDGLQPLIQKFSESLFEAKDKMSDPDQVVNVLENPEELKKKDKKDNEPPTENSQPLK
                     Q"
     gene            3539..4063
                     /locus_tag="EYO68_11230"
     CDS             3539..4063
                     /locus_tag="EYO68_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08415.1"
                     /translation="MRYASNSPKVTLFPFLSVLLSTMGILAFLSISFLLVVPESADSP
                     REPKRLQFEWVGAPGYVKPIFIRCYADRLEYYNLFENREYSLSLDELLNQLQGENPEL
                     LSYLVQLFQLNTDIKKKFGKTEYYPLLLVYPDGVLTSELLMVAIEKIGGLNFGLEPML
                     PKWEVPYQGLNSEG"
     gene            4075..6756
                     /locus_tag="EYO68_11235"
     CDS             4075..6756
                     /locus_tag="EYO68_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC08416.1"
                     /translation="MKKLFLMRIIVFGILWFVTGCQGIFSPAPDKQEIRLRNAIRIHP
                     VHELGYVRLAQYLKTQGRYSKTFSVLRTGQQHIPDSIALIRMEGGLFQGLGYYRESQE
                     YYTEQITKHPDEPLLFLDRAQLYLRLEKQQLALNDARKALSLKPDLFEALYLIGVILD
                     RRTASNVPDQSEQALDALIAASKINGRNPDLWLRISTLWERRGETHQARLAMLRAVEL
                     SPESKLYLRRYADLQEKELDLTSQKYSDEISESLHQTLQHMLKLFPDDIWVHAHMGNL
                     AWTQEKFVLAETHLLRALESKSPYPWANFRLGMVYLSQKKWESALQAFEEGLKSDPKN
                     DWAILQTGHALEMLGKNEEAITRYERLMEKGPDDLVVVNRLNQLYWNEFLFEKGEEVL
                     LRGLEKFPAETELIEKLLAYYESHRLFEKAAKIILGFIEAKPDNSAALAKLGFYEKNL
                     KHPEKALYWFKKALSSSPDFEWAHVQQIGILLNTEREDEAENALNDFLKLKPNSEWAL
                     LELSQLKLKQEHFAEAEELLEKNLAKYQDSAGILQTQGRLYELQKRWKEAENIFKKLL
                     TIRPNNSLLLTHLGFTQWKLKKTEEARQNITHALYENPGSLWAWNLYLLLLPEAQQNR
                     WIGNDFKMLLPVLGALASKRTEEAWQEITTVRTDPFTRQVLKNMHYLLEEAPQEIKMD
                     PQDMTSKQLSPWINEQWGYFHEMLGNRELAARHFEVVLEALPDNAWIHARLGWVYERL
                     ENLEKSKQHYSLFLQKHPQAFDVSFRLANVYTLTGNEAKTIELYELIAAHRPEHDLVL
                     NNLAWLYLTAQDRQVRNLEKGMELALKSVELNPTIDSLDTLAEAYFQSGDRIKALETI
                     HKAAREVDYPPNRHSYLRKQLLRFRKGEQDSSPPALT"
     gene            7011..7745
                     /locus_tag="EYO68_11240"
     CDS             7011..7745
                     /locus_tag="EYO68_11240"
                     /inference="COORDINATES: protein motif:HMM:PF02591.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08417.1"
                     /translation="MPQTIESLIKLAETDRDLQKYIFAKSHLERQIDTARSVVDQHQK
                     TIEQKKDKFELLSAECRDVNNNLQIQEELISRLDSQVPKIRNEKEFATSKNQLEEARK
                     ILGLLEDKMLDLDLKKEDLEKEIETINNRLSESNTEFKQETSGLLKKQAKAEKKIESL
                     EPRRIRLLKKVPQKIKRFYERCQDSGISTPICAIEDKSCSGCNMVLLPQLVNELMANT
                     DSHKNCPNCSRILYFPEVEEEEASSA"
     gene            7809..8105
                     /locus_tag="EYO68_11245"
     CDS             7809..8105
                     /locus_tag="EYO68_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08418.1"
                     /translation="MKKYLILLVLSLTFLPGCESSAPSRFTYPSEKAMEVRYLCRLPY
                     LDAKAPHQSWSKSTPAWLIINESKVIIFFENDRTFVLSRTPENTLPPGRYRCNL"
     gene            complement(8130..9104)
                     /locus_tag="EYO68_11250"
     CDS             complement(8130..9104)
                     /locus_tag="EYO68_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HIC08419.1"
                     /translation="MQAITLDEIEAAARIVYAEMQATPQYCWPLLCERLGTEVWVKHE
                     NHTPIGSFKIRGGLVYFAHLAKSSEMPKGVVSATRGNHGQSVGFAARRYGIPATIVAP
                     HGNSVEKNAAMRAFGVQLIEHGEDFQAAREYAKDLAHEKSLQMIPSFDPLLVTGVATY
                     SLELLRAVKDLDVVYVPIGLGSGICGMLAVRDALELNTEIVGVVSAHANTYAESFVSR
                     SSIESPVSTKIADGMACRVPEKSALELIWKGVERIVEVTDNEIADAMRMLYECTHNVC
                     EGAGAAAIAAALQEASKIKGRKVAVIASGGNVDRDVFVSVLKGETFVS"
     gene            complement(9104..11284)
                     /locus_tag="EYO68_11255"
     CDS             complement(9104..11284)
                     /locus_tag="EYO68_11255"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIC08420.1"
                     /translation="MNNSTFTTQGGIKIEKSITPLDAEHALDKIYQYIDTKKGALFVS
                     NYEVPDRYSRWDLGFVHPALELIARKRQFEINALNPNGTRLLKLIAPALQNHPHLESL
                     VFGTDPNLPAEKISGTVKPRPDFFAEEERSRQPSVFSVIRKIFELFRSVDPYLGLYGA
                     FGYDLVYQFENIEARHKRDPEQTDCHLFLPVELVVVDRQKEEASKIEYHIETPDGMTE
                     SWWNTGEEFQVAEGKADGKINCDHEAGEFEQKVAKIQEGCRRGDFFEVVLSQSFSTGF
                     AEQPSTLFKRICEQNPSPYSFLINMGKEQLVGASPEMYVRVKEERFETSPISGTVPVG
                     GDPMETAERIKALISSEKEESELTMCTDVDRNDMARICEPGSVHLIGRRLLERYSRLI
                     HTVDHLEGVLNKDRDAVDAILTHMWACTVTGSPKPVAMQTIENMENSPRGWYSGCIGF
                     LWFNGFVSTGMTLRTVHLKNGRASVRAGATLLYDSDPATEERETHIKASAFLGATLGS
                     KPAAAEDIDLPQTGGEKTVLFVDNQDSFVHTLASYVRQTGAKVITLRSGFPYKMLDEI
                     APDLLFISPGPGFPSEQNVPELVGEALAREIPIFGVCLGHQGIAEHFGAKLLTLEHPV
                     HGKSAEIFHNAESFYSGLPNPFSAGRYHSLYLDSVSLPDCLDVTAKTDDGLIMGLRHK
                     TLPVASVQFHPESILTLKDQAGLRMINKVMSELIGESRQKEGTK"
     gene            complement(11317..11949)
                     /locus_tag="EYO68_11260"
     CDS             complement(11317..11949)
                     /locus_tag="EYO68_11260"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIC08421.1"
                     /translation="MRTNHTTVYPKVKICGLTSPEQALSCAELGADWIGLNCWPGSSR
                     YISPEEVRKIVSVLPESVTTVGIFVNESPESLEKIMIETHLDLAQLHGDESVELTAKL
                     GVPFYKAFRVSAEFKLEQIQEFGREYFLLDAFSINHYGGSGQKIDWDLASTASGLGKL
                     ILAGGLTPENVAEAVKIVQPWGVDVCSGVESEPGIKDLWRVEKFIRNLRN"
     gene            12053..12361
                     /locus_tag="EYO68_11265"
     CDS             12053..12361
                     /locus_tag="EYO68_11265"
                     /inference="COORDINATES: protein motif:HMM:PF02594.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HIC08422.1"
                     /translation="MSVHSSAYFWNGDTLVLHIFLQPRASQNEWAGRHNGRIRLRVTA
                     APVDNQANRECVKFISKSLKTAKTNVKVVRGQTSRFKTVEIHKPDPKSWKKNLEGLDN
                     "
     gene            12392..>13674
                     /locus_tag="EYO68_11270"
     CDS             12392..>13674
                     /locus_tag="EYO68_11270"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIC08423.1"
                     /translation="MIHYLKTQITTICPFRFVKIPCPKVTPKLRFWSIFSLLTSLFFY
                     LVTGTASAQCLTSGLTEDEQLAMTKGLFEDELFSASSESSKCFLEEFKDSAAREEIFF
                     LRAEALRKGGDLQGAIKAYDELKKNFPSSKTYLDNSMLQKGISLALTRNYTASIRTLK
                     LLLLDFPTTKYRDEALYWLGYAASFQAESLRKKKNKDALLFYQDSTLHFVKVEPELLN
                     LKQQQERLHLMGRSWWFLKDFTKAEEAWSQYLKNSKSIKPEQALNLKYQLATGFQQEK
                     NYKKAERWFARILTKHPDSKLAVVSAFLRAEMAYAVSVQETKTESLDSKAVSILIKHY
                     KLYLNKNDNKYQALVYYRIGVLQQPHRPNEAIHAFQKYLSTKDKTYAAEVHYRLGYLF
                     FNTKQQQKAIQIFEKYLRTKDKTYTNEVLYQLGFL"
BASE COUNT         4153 a         2933 c         2718 g         3870 t
ORIGIN      
        1 agcaaaaaac gggcaatcaa catggctaaa gtcgcaccaa tggaactggc gaaagaaacg
       61 atcactgttc ctgcaaataa tccgaaaaaa gccccacctc ccaaagtcat ccagactgca
      121 cctggaagtg aaaatgcagt aatgactatg taaaatagaa aataactgcc caacaccaaa
      181 atgggatttt tctcgtagga tgaaatgaaa aatgctttgg aggattttag atattcgaga
      241 gtcaggtact tttgtaaatc gaagacgaaa aacaaagcaa caacaatggt gaagacggct
      301 ataagcaaca aaattttgat ttgcttattc ttctcaggca tagaattgaa tcttgtctat
      361 tttatgtaat tcagaattta catagattac atctcagttc aaaggatgat ctacaataat
      421 tttacacgct taccatgaat tgcgccccat gtggttacac agtaaggaac acaatatgta
      481 agcataattt taaaataatt aatggattca cctttaagaa tgacatcacc atgattgatg
      541 gtggctagga tagtccccac tactaatgcg gtcataaggg cttttttggg agtgccgtcg
      601 ccaaaagcta attgaaaaaa gtcagggttt gctgtttgtt tcatcatttt ttatttctaa
      661 gattatatga gatagaatgt cataagtgac ggtcccgcaa taagtatgca attcacaatt
      721 tgtgatttag atagtaagga aaattattta tgatattagt gtagtagtta atacataatt
      781 tctggtttct ctcgaaatga cacggtttta cgacttttta tgtcggtatt atgatttatc
      841 tttcagtaga cataataatc tttcaaattg gatagaacct tttttgtatt ttcaaaaaaa
      901 ggatttatga ctagaacgta atcacttttt tcaaagggta atagtttgat agccatctgc
      961 aaagagtctg gcaatgcttg gatgtccttt atattcatca aacaaaggca gctcttcctt
     1021 tttcaacaaa tctttttctc caccaaacga ggtgtcgcaa taatcacaaa ctcctgcgat
     1081 tactcctgaa cttttcagtg attgggaaag cggagtcatt ttatgttctt tgctaaattc
     1141 ttcaatccac tttgtaccgc ctccatcaaa aatgagctgc acgtcacgcc tttattccaa
     1201 caactaattg accacatgca ccctgaatat cctgtccttt ggaaattctt attggagcaa
     1261 caacaccatg atctagaagg tacttttgga aattacgtga ctgctccact gaaggtgatt
     1321 gatatttgct gcccggagca gagttgaagg gaatcaaatt tatcttgaat ttcataccgt
     1381 gcagcaaact aatcaatccc tttgcatctt ttattgaatc tgtcaaatca cgaatcagaa
     1441 tgtactcaaa tgtaatacgt ctccgtgatt ccaaagggaa ctctcgacaa actttaatga
     1501 gcttttccag attgtatgga atgttgacgg gcatcagttt tgaacgaaca tcatcagtta
     1561 caccattcaa agaaattgcc agattggcct tgattttttc tttcccaaaa cgccggattt
     1621 tcggcagtaa tccggaagtg gaaacagtga tgcggcgctg tgaaaaatta aaaccatgct
     1681 catccgtaag aatttccaga gcctgcatga gattttgata attatgaaag ggctcgccca
     1741 ttcccataaa aacgatattc cgaatatttt tcccctcggg ctgatcattc cagactgtca
     1801 atacttggtc gataatttct gccgttttta gatttcttac caaacccata gttccagtca
     1861 tgcagaaatc acagcccatt ccacatccta cctgagatga aacacaaact gtattatgac
     1921 tggtatgcgg cataaacacg gattctacgc taagtccgtc atccagtaac aaacgatatt
     1981 taattgaacc atcctgagat tgttgtgttt cagccttttc gagtgagctg atccagaaat
     2041 tctcttccat aatttttcgg gtttcccggc caatattgtg catttcggaa aagctctccg
     2101 cctttttctg atagagccaa taaaatattt gatctgccct gaattcctgc accttactgt
     2161 gttttatgga tttttcagcc atccacgcac gtaaattatg tcgtgtccag tttttaatgt
     2221 tgtacagttt tgacatttta tatttggaaa tgtaactatt taatgaatat gttattcttc
     2281 atggtaagta aatgcacccg agtggtcaag cgagagctcc ttaaatttaa aaaatgctca
     2341 tacaaaatat tggttaactg atgaaatgtt taaaatgcgg atggtccaat tctgacactg
     2401 ctgcctattg tacaaaatgc catgcatacc tgtcagaaca gccgggcatg gagtcaatag
     2461 atgcaattaa agctctgatt ttaagtctgt tttttaccgg aattttctat ctcgttttcc
     2521 cgattcctgt catccggagt gaatactggc tgcaattatt ttcaggtcat atctcagaaa
     2581 ctattgttgc ccttgtgctt tggtccctgt ttttgatttt tttcaagtgg cggcagtttc
     2641 gtcaacaact ccgggcctat gaggctttca ggaatcagat catgcacgac tctttatcaa
     2701 aaggcattta tgtcagggat gtggacgaga ggatattgga aatcagtcag tttatgcagg
     2761 agcaaaaagt aaagaaattt caggacagcg tcattttccg gagagtacgc cgcacattaa
     2821 gacacctcaa agccattcct aaaaaagaag aaataacttc aattctcaat tatcaggcag
     2881 acattgatca caacagaatg caaagcggat atgctctttt aaacgttttt atctgggcaa
     2941 ttcccattct tgggtttatc ggtaccgttt ttggaatcgg acagtcagtc ggcgaatttt
     3001 cagattttat ccgcagcacc ggcggcactg atttgaacac gcaaatgcgc tcggctttgg
     3061 gaggagttac cagtggatta tcagttgcat tcagcactac atttattgca ttggttggag
     3121 tagttccgat catgatgctt gcttcatctt tgcgtaaacg agaggaagat ttgcttctca
     3181 gtgttgaaga ttactgtctg gaagatttac tgccgaattt acatgtacgt cctggagatg
     3241 agatgctgga tgacacagtt agtgaacatt tggaacagat ggcagagttt tctgagaatt
     3301 ggcgcaaaca ggtttcacct gttcttgaat ccgtggaacg tcattcaaaa ggatttgcag
     3361 cgcaagttga tggattacag cccttgattc aaaaattcag tgaatcccta tttgaagcaa
     3421 aagacaaaat gagtgacccg gatcaagtgg taaatgtcct tgaaaaccct gaagagttaa
     3481 agaaaaaaga caaaaaagac aatgaaccgc caaccgaaaa ttcccaacct ttaaaacaat
     3541 gaggtatgcc tctaattctc ctaaagtaac acttttccca tttctgagtg tcctgctcag
     3601 tacaatggga attttagcat tcctttcaat tagtttttta cttgttgttc ctgagagtgc
     3661 agattctccc agggagccca aacgtttgca atttgaatgg gtcggtgcac ccggatatgt
     3721 caaacccatt tttatccgct gttatgcgga tcgtttagaa tattacaatt tgtttgaaaa
     3781 ccgggaatac agcctctctc ttgatgaatt gctgaaccag cttcaaggtg aaaatccgga
     3841 actattgtca tacctagttc agctatttca gctaaatact gatattaaaa aaaagtttgg
     3901 taaaacagaa tattaccctc ttttactggt ttatcctgac ggcgtgttaa cttcagaatt
     3961 attgatggtt gcaattgaaa aaattggagg attaaatttt ggactggaac caatgctacc
     4021 gaaatgggaa gtaccatatc agggactgaa ttctgaaggc tgatatttga tgttatgaaa
     4081 aaacttttct taatgagaat aattgttttt ggcatcttgt ggtttgtcac cggctgtcag
     4141 ggaattttca gtcctgctcc agataaacag gaaattcgat tgcgcaatgc tatccggatt
     4201 catcctgttc acgaactcgg ctacgtccgg ctggctcaat atctgaaaac tcagggacga
     4261 tactctaaaa cattttcagt actccgtacc ggacagcagc atattcctga ttcgattgcg
     4321 ctcattcgaa tggagggagg tctgtttcag ggtttgggtt attacagaga atcacaagaa
     4381 tattacacag aacaaatcac taaacatccg gatgaaccgc tcctttttct ggatcgagcg
     4441 caactgtacc tgcgtctgga aaaacagcag cttgctttga atgacgcacg caaagcactt
     4501 tctttgaaac ctgatttgtt tgaagctctt tatctgattg gtgtgatttt agaccggagg
     4561 actgcctcaa atgtaccgga tcagtcagag caggcacttg atgctttaat tgccgccagc
     4621 aaaataaatg gacggaatcc tgatctctgg ctgagaattt caacattatg ggaaaggcgt
     4681 ggtgaaactc atcaggcaag actggcaatg ctccgggcag ttgagctttc tccggaatca
     4741 aaactttacc ttcgccgtta tgcggatttg caggaaaaag agctggacct aaccagtcag
     4801 aaatactctg atgaaatctc ggaatccctg caccagactc ttcaacacat gctcaaattg
     4861 tttccggacg acatttgggt acacgcacat atggggaacc tggcctggac acaggaaaag
     4921 tttgtgctgg cggagacaca tttactgcgt gctcttgaat caaaatctcc atatccctgg
     4981 gctaattttc gattgggtat ggtttatctc tctcaaaaaa aatgggaatc tgcactccag
     5041 gcttttgagg aaggtctaaa atccgatcct aaaaacgact gggccattct gcagacagga
     5101 catgccctgg aaatgctggg aaaaaatgag gaagcaatta cccgttacga aaggctgatg
     5161 gaaaaaggtc cggacgacct ggttgtagtt aatcgtctga atcaattgta ttggaacgaa
     5221 tttttgtttg aaaaaggtga agaggttctt ttgcgtggac tggaaaaatt tcctgcagaa
     5281 acagagctaa ttgaaaaact acttgcttat tatgaatctc atagactttt tgaaaaggca
     5341 gcaaaaatta ttttgggttt tattgaggca aaaccagaca acagtgctgc acttgcaaag
     5401 cttgggtttt atgagaaaaa cttgaaacat ccggaaaagg ctttgtactg gtttaaaaaa
     5461 gcactctcaa gttctccgga ttttgaatgg gcacacgtcc agcaaattgg aattttatta
     5521 aacacagaac gcgaagatga agcagaaaat gcgctaaatg attttctcaa acttaagccg
     5581 aattcagaat gggcgctgct ggaactgtct cagctgaagt tgaaacagga acattttgct
     5641 gaggcagaag aactgctgga aaaaaatctt gcaaagtacc aggattcagc gggaatactg
     5701 caaactcagg ggcggctgta tgaattgcag aaacgctgga aagaggcaga aaatattttc
     5761 aaaaaactat taacaatccg cccaaacaac tctttgctgc tgacgcattt gggttttacg
     5821 caatggaaac tcaagaaaac agaagaagcc cgtcaaaaca ttactcatgc tttgtatgaa
     5881 aatcccggga gtctctgggc ctggaatctt tatttgcttt tgctgccgga agcacagcag
     5941 aatcgctgga tcggcaatga cttcaagatg ctcctgccgg tcctaggtgc attagccagc
     6001 aaaaggacag aagaagcctg gcaggaaatt actactgttc gcacagatcc ttttacacgg
     6061 caagtactga aaaatatgca ctatttgctc gaagaagctc cgcaagaaat taaaatggat
     6121 ccgcaggata tgacctcaaa acaactctcc ccctggataa atgagcaatg ggggtatttc
     6181 cacgaaatgc tgggaaacag agagcttgct gcacgtcatt ttgaagtcgt tttagaagca
     6241 cttccggaca atgcctggat acacgcacgc ttgggctggg tctacgagag gctggagaat
     6301 ctggaaaaaa gcaagcagca ttacagcctt tttttacaga aacatccgca agcatttgat
     6361 gtcagtttcc ggctggcaaa cgtctataca ttgacaggca acgaagccaa aaccattgag
     6421 ctttacgaat taattgcagc tcaccgtccg gaacatgact tggtgctgaa caaccttgcc
     6481 tggctgtatt taactgctca agacaggcag gtacgtaatc ttgagaaagg tatggaatta
     6541 gccttgaaat cagttgaact gaatcctaca attgacagtt tagatacatt agccgaggcc
     6601 tactttcagt ctggagacag aattaaagcc cttgaaacta tccataaagc tgctcgggaa
     6661 gttgattatc cgccaaaccg tcattcatac ctgcgaaaac aattgctgcg cttccgcaaa
     6721 ggagaacagg actccagccc accagcgtta acatgattca tgttatgttg aaatgatttt
     6781 actctaaagc tgtttaggtc agcaaaacac aatgaattgt acaataattt gatgaaggtg
     6841 tgcttaactg atttaaatgg gaaacgacag ctaacagagg ttccatgcag aatcacagac
     6901 caacatggat cccgcaaaaa ttaaaaccaa attacacttg taaattcaat atttttctga
     6961 gacaattaca ggattaattt ttccaagtga ctttatcttt caaaacttcc atgccacaaa
     7021 ctatagaatc attgattaaa cttgccgaaa ctgaccgtga tttacagaaa tatattttcg
     7081 caaaatctca tttggaaaga cagattgata cagcacgttc agttgttgac caacatcaaa
     7141 aaacaatcga acaaaaaaaa gacaaatttg aactattgag tgctgaatgc agggatgtaa
     7201 acaacaatct tcaaattcag gaagaattga tttcccgcct ggacagtcaa gtacctaaga
     7261 tacgcaacga aaaagaattt gcaaccagta aaaaccaact ggaggaagca cgaaaaattc
     7321 taggtctgct ggaagacaag atgctcgatc tggatctgaa aaaagaagat cttgaaaaag
     7381 aaattgaaac tattaacaac cggctcagtg aatcaaatac agaatttaag caggaaacat
     7441 ccggtctgct gaaaaaacaa gcaaaagcag aaaaaaagat tgaatctctt gagcccagac
     7501 gcatacgttt actgaaaaaa gttccacaaa aaatcaaacg tttttatgag cgctgccagg
     7561 acagtggaat ttcaacccca atctgtgcca ttgaggataa aagctgcagc ggctgtaaca
     7621 tggttttatt gccccagctt gttaacgaac tgatggcaaa cacagattcc cacaaaaatt
     7681 gccctaattg ttcgcgcatt ttgtactttc ctgaagttga ggaagaagaa gcttcctcgg
     7741 cctgatattt cttatcatgc atgattgtat tcggtgttga tcaatctctg cttcaacaaa
     7801 atacaatcat gaaaaaatat ttaatactgc ttgttctttc tttaacattt ttgcccggct
     7861 gtgaatcttc agccccttcc cgttttacat atccttctga gaaagcaatg gaggtacgct
     7921 atctttgccg cttaccatat ctggatgcaa aagcgccaca tcaatcctgg agcaaatcaa
     7981 cacccgcctg gctcattatt aatgaaagca aggtcatcat tttttttgag aatgaccgta
     8041 cctttgtgct ttccagaact ccggagaata cccttcctcc aggcaggtac cgctgcaacc
     8101 tctgaaagtt actggctgaa tctacagtgt tatgaaacga aagtttcacc ctttagcaca
     8161 gaaacaaaaa catctcgatc gacatttccg ccggaagcaa ttaccgccac tttgcggcct
     8221 ttaattttgg acgcctcctg tagtgctgcc gcaattgctg ctgctccagc cccttcgcaa
     8281 acattatgag tgcactcata caacatccgc atggcatctg caatttcgtt atctgtgacc
     8341 tcaacaatcc gttcaacacc tttccaaatc aattccaacg cagacttttc cggaactcgg
     8401 caggccattc cgtcggcaat tttggtactc accggtgatt caatggaact tctgctaacg
     8461 aatgactccg cataggtatt ggcatgtgct gatacaactc cgacgatctc cgtattcaat
     8521 tctaatgcat cacgtactgc aagcattcca cagattccgg atcccaatcc aatgggaacg
     8581 taaacaacat caagatcctt gaccgctctc agcaattcca aactataggt ggcgacacct
     8641 gtaactaata gtggatcaaa cgaaggtatc atttgcagtg atttttcatg tgccagatct
     8701 ttcgcatatt cacgggctgc ctgaaaatct tcaccatgct ctatcagctg cacaccaaaa
     8761 gcacgcattg cagcattttt ctccacactg tttccatgtg gcgctacgat tgtagccgga
     8821 attccgtagc gccgagcagc aaaaccaaca gactgcccat gatttccgcg ggtcgcactg
     8881 acaactccct ttggcatttc tgaggatttc gcgagatggg cgaaataaac taatccaccc
     8941 cgtatcttga aagaaccaat tggtgtgtga ttttcgtgct tgacccagac ttctgtgcct
     9001 aaacgttcac aaagcagcgg ccagcaatat tgaggagtcg cctgcatttc cgcataaacg
     9061 attcgggcgg cagcttcgat ttcatcaagt gtaattgcct gcatcatttt gtgccttctt
     9121 tctgtcttga ttctccgatc aattcactca tcaccttgtt tatcatcctt aatcctgcct
     9181 ggtctttgag tgtcagaatg gattccggat gaaattgtac tgaagccacg ggcagtgttt
     9241 tgtgccggag ccccattatc aagccgtcgt ctgttttggc ggtaacatcc agacaatccg
     9301 gaagtgaaac tgaatccaaa taaagactgt gatagcgtcc tgcgctgaag gggttaggaa
     9361 gcccggaata aaacgattct gcattgtgaa aaatctcagc ggatttgcca tgaaccggat
     9421 gctcaagagt caacaatttt gcaccaaaat gttctgcaat cccctggtga ccaagacaca
     9481 ctccaaaaat tgggatttca cgtgcaagcg cttcaccaac aagttccgga acattttgtt
     9541 cacttggaaa ccccggaccc ggcgaaatga aaagtaaatc cggagctatt tcgtccagca
     9601 ttttataagg aaatccggag cggagagtaa taacttttgc accggtttgc ctgacataac
     9661 ttgctaatgt gtgtacaaaa ctgtcttgat tatcgacaaa taaaaccgtt ttttctccac
     9721 ccgtttgcgg caaatcaatg tcttcagcag cagccggttt actgcccaaa gtcgcaccta
     9781 aaaaggctga agccttgatg tgtgtctccc gttcttctgt agccggatct gagtcataga
     9841 gcagtgttgc accggcacgc actgaagctc ggccgttttt aagatgaacc gtacgcaatg
     9901 tcattccggt gctgacaaaa ccgttaaacc acaaaaaacc aatacagccg ctgtaccagc
     9961 cgcgcggtga attttccata ttttcaattg tctgcatggc aacaggtttt ggtgaacctg
    10021 tcacagtaca ggcccacatg tgcgtcaaaa tagcatcgac tgcatctctg tctttattta
    10081 aaacaccttc caaatgatca actgtatgaa tcaggcgtga ataacgttcc agcaggcgcc
    10141 gtccaattaa atgaacacta ccgggttcac aaattctggc catatcgtta cgatccacat
    10201 ccgtacacat agtgagttct gattcttctt tttccgaaga aatgagtgct tttatacgtt
    10261 ccgctgtttc catcggatca cctccgaccg gtactgttcc tgaaatggga ctggtttcaa
    10321 aacgttcttc cttgacacgt acatacattt ccggagatgc accaaccagt tgctctttac
    10381 ccatgttgat caaaaaactg taagggctgg gattttgttc acaaattctt ttgaacaaag
    10441 ttgaaggctg ttctgcaaaa ccagtagaaa aagattggga cagtacgact tcaaaaaaat
    10501 ctccacgccg gcagccttct tgaattttgg ccactttctg ttcaaactca cctgcttcgt
    10561 gatcacagtt tattttacca tcagcctttc cctcggcaac ctggaattcc tcgccagtat
    10621 tccaccagct ttcagtcata ccgtccggag tttcaatgtg atattcaatt ttggaagcct
    10681 cctctttttg ccgatcgaca accaccaatt cgacaggcaa aaacaaatgg cagtcagttt
    10741 gttccggatc ccgtttatgt ctagcttcaa tattttcaaa ttgataaacc aaatcgtaac
    10801 caaatgctcc atacaatcca agataaggat caacactccg gaacagttca aatattttgc
    10861 gaataacaga aaacacgctt ggctggcgac tccgttcttc ttccgcaaag aaatccggcc
    10921 ttggtttcac agtaccggaa atcttttctg ctggaagatt cggatcagtt ccaaaaacca
    10981 gtgattcaag atggggatga ttttgtaaag caggagctat caatttcagc aggcgcgttc
    11041 cattcggatt cagcgcgttg atttcaaact gtcttttcct ggcaatcagt tccaatgcag
    11101 gatgcacaaa acctaaatcc cagcgggaat aacggtccgg aacttcgtaa ttactgacaa
    11161 aaagtgctcc ttttttagtg tcaatatatt gatatatttt atcgagtgca tgttcagcat
    11221 ccagcggtgt aatcgacttt tcgattttga taccgccctg tgttgtaaaa gttgaattat
    11281 tcatggcttc aagtaaattt taagtgttta aagaaatcaa tttctaaggt ttcgaataaa
    11341 tttttcaact cgccacaaat ctttaattcc aggttcgctt tctacaccgc tgcaaacatc
    11401 cactccccac ggttgtacaa tctttactgc ctctgcaaca ttttccggag tcaaccctcc
    11461 tgccagaatc agctttccca aacctgaagc agtacttgcc aggtcccagt caattttttg
    11521 tccggaacct ccgtaatgat ttatgctgaa ggcatccagt aaaaagtatt ctctcccaaa
    11581 ctcttgtatc tgttccagct taaattcagc agacacacga aatgctttat aaaatggtac
    11641 acctaattta gcagttaatt caacagactc atcgccatgc aattgtgcca agtccaggtg
    11701 tgtttcaatc attatttttt ccagcgattc cggtgattca ttgacaaaaa tacccacagt
    11761 tgtcaccgat tccggaagca cacttacaat tttgcggact tcttccggag aaatgtaacg
    11821 ggaagaacca ggccagcagt ttagtccaat ccagtctgca cctaattctg cacaggacaa
    11881 agcctgttca ggactggtta gcccgcaaat tttaactttt ggataaacag tggtgtgatt
    11941 cgtcctcatt ggcttctgga ttggtttttt ttaactataa cgttataata tgttgttgtt
    12001 ctaagatacg aatacttaca gaattagcaa cgaaaaaatt ccggaaaact agatgtctgt
    12061 tcatagttct gcatatttct ggaatggtga cactttggta ctgcacattt tccttcaacc
    12121 acgtgcttca caaaatgagt gggcaggccg acacaacggg cggatacgtc ttcgtgttac
    12181 agcagcaccc gtcgataatc aggccaatcg ggaatgtgtg aaatttattt caaaatcatt
    12241 aaaaactgca aaaacaaatg tgaaagtggt tcgaggtcaa accagtcgtt tcaaaactgt
    12301 ggaaatccat aaacctgatc cgaagtcctg gaaaaaaaat ttagaaggct tggataatta
    12361 gattatctgc attgtagaac ttaaaaagct aatgatccac tacctgaaaa cgcaaataac
    12421 aactatttgt ccgtttagat tcgtgaaaat accctgccca aaagttactc caaaattacg
    12481 attctggagc atattttctt tactgacaag cctctttttt tatttagtaa ccggaactgc
    12541 gtcagcacag tgcttaacca gcggtctgac tgaagatgag cagttggcta tgacaaaagg
    12601 attatttgaa gatgagctgt tttccgcatc aagtgagtcc tcaaagtgtt ttcttgaaga
    12661 atttaaggat agtgctgcac gtgaggagat ttttttcctt agggcagaag ccttaagaaa
    12721 gggaggcgat cttcaggggg caatcaaagc ttatgatgaa ctgaaaaaaa actttcccag
    12781 cagcaaaacc tatttagaca attcaatgct ccagaaagga atttcactgg ccctcactag
    12841 aaattatact gcttctatca ggaccttaaa attgctgctg cttgattttc ccacaacaaa
    12901 atacagagat gaagcacttt attggctggg ttatgcagct tctttccagg cagagtctct
    12961 gcgcaaaaaa aaaaacaaag acgctctgct tttttatcag gattccacac ttcacttcgt
    13021 taaagtagaa cctgaattat taaatctgaa gcagcagcag gaacgtttgc atttaatggg
    13081 acggtcatgg tggttcctga aggactttac taaagcagaa gaagcatgga gtcaatactt
    13141 gaaaaactca aagtcaatca aacctgaaca ggctttgaat ctaaaatacc agttagccac
    13201 aggctttcag caagagaaaa actacaaaaa agctgaaaga tggtttgcgc gcattttaac
    13261 aaaacatcca gactcaaaac tggctgttgt gtctgctttt ttacgcgcag aaatggcata
    13321 tgcagtttca gtacaagaaa caaaaactga atcattggac tcaaaggcag taagtatact
    13381 gattaagcac tacaaactat atttaaataa gaatgataat aagtatcaag cacttgtcta
    13441 ttacaggatt ggtgtgttgc agcaaccgca cagaccaaat gaagccattc atgcttttca
    13501 aaaatattta agcacaaaag acaaaactta tgcagctgaa gttcattatc gcttaggtta
    13561 tttgtttttt aacaccaaac agcagcaaaa ggctattcag atcttcgaaa agtatctccg
    13621 taccaaagat aaaacttaca ccaacgaagt cctttaccag ctaggatttc tcta
//
LOCUS       DTVD01000308            3562 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_9953_length_3562_cov_237.513259, whole genome
            shotgun sequence.
ACCESSION   DTVD01000308 DTVD01000000
VERSION     DTVD01000308.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9953_length_3562_cov_237.513259"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..3067)
                     /locus_tag="EYO68_11275"
     CDS             complement(<1..3067)
                     /locus_tag="EYO68_11275"
                     /inference="COORDINATES: protein motif:HMM:PRK06148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIC08424.1"
                     /translation="MNKSLPELERPEFSEQEAGLLLEENYGLCCTLEELPGERDRNYL
                     AQEHNGESYVLKISNSCETLEFLKVQNNALESAAMLLEKGRIPSVYPNKNGEPLSRVR
                     STNGSLHWLRLVPYVDGLSMAEYRPHTREFLLELGAMCGTVTKALHKIPLRTLDRRLL
                     WEMHNVQDTLNEYLTWIKDKKLRNRVSRSLDLYKRTMEPLESKLRRGWIHNDFNDYNV
                     LVLPKLAGTPDLGLIDFGDMTHSYLVAEPAVACAYAMLDKPDPLEAAVHLIRGFHQRF
                     PLEENELEILFPMILMRLCLSLTIGAFQQQNDPKNEYLGISQQHACELLERLHEVNPR
                     FAYYLFRDACNMEAFPSLPEFSKWQKKVAGSFHFLLGEPLNTEKTTVLDLSAGSSFSA
                     KSEGMSLEAQQEFLDTYLREKNAEIGVGKYLEARSFYAADEFVNDSLDGHEKRTIHLG
                     IDICVPAGTVIYAPIKGVVHQIQDNKSELDYGPTVILKHQPEDGPVFYTLYGHLSWEC
                     LKQLKTGQIVSGGTALAKIGDSNENGGWLPHVHFQIILDLFDYDGNYPGVALPSRKKV
                     WCSICPDPGMMLGLGSESTAEEIDSGQLLNRRRNVFGQSLSLSYQEPLIIVRGQGQSL
                     IDSKGQFYLDCVNNVAHVGHSHPDIAKAQSNQAYVLNTNTRYLNPVNIEYAERLCGLF
                     PEPLNTCFLVCSGSEANELALRIAGTVNGQKDMIVLEEAYHGNTKANIDISPYKHNGP
                     GGTGPPEWVHQIPMPYLYRGLYRDPATAGKLYADEVLKICEKVSGQGKTPAAFICESM
                     LGCGGQVPLPDGFLKQSYQHVRQYGGLCIADEVQVGFGRAGKHFWSFELQNVVPDIVT
                     LGKPIGNGHPLGAVITTQKIAEEFANGMEYFNTFGGNQVSCSVGMAVLDIMENEGLQQ
                     NALETGSWLKEKLEMLKNVFPLIGDVRGEGLFLGVELVLDPETREPAPLHADYLVERL
                     KSRKILLSTEGPGHNVLKFKPPMVFNHSDAQHLLVELQQVLRESPMQQNL"
BASE COUNT          950 a          857 c          751 g         1004 t
ORIGIN      
        1 taagattttg ctgcatcggg ctttcgcgca acacctgttg cagctctaca agcagatgct
       61 gtgcatcact atggttgaaa accatgggtg gtttgaattt caggacgttg tgtccaggac
      121 cttcggtact gagcagaatc ttgcgtgatt tcagcctttc gaccaggtaa tccgcatgaa
      181 gcggcgcagg ctccctcgtt tcaggatcga gaaccagttc aactcccaga aacaaaccct
      241 caccgcgaac atctccgatc agcggaaaaa cgtttttcag catttccaat ttctctttca
      301 gccagcttcc tgtttccagc gcattttgct gaagcccctc attttccatg atatccagaa
      361 ccgccattcc cacagaacag gaaacttggt ttcctccgaa agtattgaaa tattccatgc
      421 cgttggcgaa ttcttccgca atcttctgtg ttgtaattac cgcaccaagt ggatgtccgt
      481 ttccgattgg cttaccgaga gtaacaatgt ccggtaccac gttctgcaat tcaaatgacc
      541 agaaatgctt acctgcacgt ccaaaaccta cctgaacttc atcggcaatg cagagtcctc
      601 catattgcct cacatgttgg taggactgct tcaagaagcc atccggaaga ggaacctgcc
      661 ctccgcagcc aagcatggac tcacagatga acgctgcggg ggtttttccc tgtccggaaa
      721 ctttttcaca gattttgagg acctcatcag cataaagctt tcccgcagta gctggatctc
      781 gatacagccc gcgatacaga taaggcatcg gaatctgatg tacccattcc ggaggaccag
      841 ttccacccgg accgttgtgt ttgtagggac tgatatctat attcgctttt gtattgccgt
      901 gatatgcttc ctcgagtaca atcatgtcct tttgtccgtt gaccgtgccg gcaatccgca
      961 gggcaagttc gtttgcttcg cttccggaac agacaagaaa acaagtgtta agaggctcag
     1021 ggaacaaccc gcaaagacgc tccgcatact caatattgac cgggttcaga tatcgggtat
     1081 tggtgttcaa aacataagcc tgattgcttt gtgcctttgc aatatctgga tgagaatgac
     1141 ccacatgagc aacattgttg acgcagtcca gataaaactg tcctttggag tcaatcagcg
     1201 actgcccctg acctcgaaca attatgagcg gttcctgata agaaagactc aatgactgac
     1261 caaaaacatt tctccgcctg ttcagcagtt gtccggaatc aatttcctca gctgtgcttt
     1321 cgctgccaag tcccagcatc atgccgggat cgggacaaat gctgcaccag actttttttc
     1381 tggaaggcag tgctactccg gggtagttgc cgtcgtaatc aaacagatcg aggataatct
     1441 gaaagtgaac atgcggaagc cagcctccat tttcatttga gtctcctatt ttggccagag
     1501 ccgtaccacc actgacaatt tgccctgttt ttaattgctt caaacactcc cagctcaagt
     1561 gaccataaag tgtataaaac actggaccat cttccggctg atgctttaat atcactgtag
     1621 gtccgtaatc tagctcactc ttattatcct ggatttgatg gacgacacct ttgattggcg
     1681 cataaataac cgttccggca ggtacacaga tgtcaatgcc aagatgtatg gttcttttct
     1741 catgaccatc aagagagtca ttcacaaatt catcagcagc atagaatgag cgcgcttcca
     1801 aatactttcc tacaccaatt tcagcattct tttctctcag atatgtatct aaaaattcct
     1861 gttgtgcctc cagagacatc ccttcggatt ttgcagaaaa agaactacct gcgctcagat
     1921 caagtaccgt tgttttttca gtattaagcg gttctccaag aagaaagtga aatgatccgg
     1981 caaccttttt ttgccatttg ctgaattcag gcaagctggg aaaagcttcc atattacagg
     2041 cgtccctgaa gagatagtac gcaaagcggg gattaacctc atgtagccgt tccagtaatt
     2101 cgcatgcgtg ttgctgactg attccaagat attcgttttt agggtcgttc tgctgttgga
     2161 acgcacctat tgttagactc aaacaaagac gcatcagtat catcggaaac agtatctcaa
     2221 gctcgttttc ttcaagcgga aagcgctgat gaaaaccgcg aatcaggtga accgccgcct
     2281 caagaggatc gggcttgtcc agcatcgcgt aggcgcaggc aactgcaggt tcagccacaa
     2341 ggtaggaatg ggtcatgtcg ccaaaatcga tcaatcctaa atcaggagtt ccagcgagtt
     2401 ttggcaaaac tagaacgttg taatcgttga agtcattatg aatccagccg cgcctcaatt
     2461 tggattcaag aggttccatt gtccgtttgt acaaatccag tgaccggctg acccggttcc
     2521 gcagcttttt gtctttgatc caagttagat attcattcag agtatcctgc acattatgca
     2581 tttcccaaag aagcctgcgg tcaagagtac gcaaagggat tttgtgaagt gcctttgtta
     2641 cggttccgca cattgctcca agttccagca aaaactctct ggtatgagga cggtactccg
     2701 ccatggaaag tccatcaaca taaggtacca gacgtagcca gtgcagactg ccgtttgtgg
     2761 acctgacacg tgaaagtggt tctccatttt tattaggata aacgctggga atccttccct
     2821 tttcaagcag cattgccgca ctttcaagcg cattattctg tactttcaga aattcgagcg
     2881 tttcgcagga attcgaaatc tttaaaacat aagattcacc attatgctcc tgagccagat
     2941 aatttcgatc cctttctccg ggtagttctt caagtgtaca acaaagtccg tagttttctt
     3001 ccagcaggag accagcctct tgctctgaaa actccgggcg ctccagttca ggtaatgatt
     3061 tgttcatgtt tttagttgga gttattatct actctggtgt ttgaggagtt gtatacaaag
     3121 aggaaaatcc ggtttactaa tatcgtcaaa tatcaatcca ccgacttaaa aagtatctgc
     3181 tgtcatagat aagaatagca ttttagagtt caataataaa gagagtgctt caagcagaaa
     3241 ccactttttt tcaagtctta aaccgtatca taaggtagaa atgtcagata tgtgtgtttg
     3301 tttcaattac cagaatagta taaaattatt taccagcatt tttaccagca ctggtttagc
     3361 ttatgtgaat cgttttttac attttatgct atatgtccaa ctgccattcc cgctaagcat
     3421 gtctaagaca atccgttaaa accttgtcat tccctcgcat gtgggaatcg aggtgaggca
     3481 gttaaaaacc actatcttta acctgaatta ccattctcac tcaattgagg tatgaacccg
     3541 atgtggttga aaacagaatt ct
//
LOCUS       DTVD01000309            3556 bp    DNA     linear   ENV 04-MAY-2020
DEFINITION  TPA_asm: Candidatus Lambdaproteobacteria bacterium isolate
            UWMA-0124 NODE_9982_length_3556_cov_385.785204, whole genome
            shotgun sequence.
ACCESSION   DTVD01000309 DTVD01000000
VERSION     DTVD01000309.1
DBLINK      BioProject: PRJNA522654
            BioSample: SAMN10967472
            Sequence Read Archive: SRR8626074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Candidatus Lambdaproteobacteria bacterium (marine metagenome)
  ORGANISM  Candidatus Lambdaproteobacteria bacterium
            Bacteria; Proteobacteria; Candidatus Lambdaproteobacteria.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Zhou,Z., Tran,P.Q., Kieft,K. and Anantharaman,K.
  TITLE     Genome diversification in globally distributed novel marine
            Proteobacteria is linked to environmental adaptation
  JOURNAL   bioRxivorg, doi 10.1101/814418 (2019)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2019) Department of Bacteriology, University of
            Wisconsin-Madison, 4545 Microbial Sciences Building, 1550 Linden
            Drive, Madison, WI 53706, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.12.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 495.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/20/2019 14:56:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,259
            CDSs (total)                      :: 2,236
            Genes (coding)                    :: 2,208
            CDSs (with protein)               :: 2,208
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 2 of 28
            Pseudo Genes (frameshifted)       :: 11 of 28
            Pseudo Genes (incomplete)         :: 13 of 28
            Pseudo Genes (internal stop)      :: 6 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Lambdaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_3556_cov_385.785204"
                     /isolate="UWMA-0124"
                     /isolation_source="Tui Malila hydrothermal plume 10m"
                     /db_xref="taxon:2035771"
                     /environmental_sample
                     /country="Pacific Ocean: Lau Basin"
                     /lat_lon="21.9900196833333 S 176.568744183333 W"
                     /collection_date="2009-07-01"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /gene="uvrB"
                     /locus_tag="EYO68_11280"
     CDS             complement(<1..1564)
                     /gene="uvrB"
                     /locus_tag="EYO68_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIC08425.1"
                     /translation="MEFNLQSEFKPSGDQPQAIEALVNGVEGKHKHQVLLGITGSGKT
                     FAMANVVQTLQRPTLVIAHNKTLAAQLYNEFQTFFPENAVEYFVSYYDYYQPEAYVPS
                     RDLYIEKDTALNDDLDRLRLSATRSLLERRDVLIVASVSCIYGIGSPEKYEKMLLIFK
                     VGERFSRQVILERLVELLYTRNDIDFHRGTFRVRGDVIDIYLAEAESAIRVELFGDEI
                     ESLVRFDPLTGKKQMSYQHFVIYPASHYVAPAEHIPETLSLIREELRERLEDLNAQNK
                     ILEAQRLLQRTEYDLEMIEQTGSCSGIENYSRIIDRRAAGTPPATLLNYFPDDSLIFV
                     DESHMTLPQLRAMYKGDFSRKSTLVEHGFRLPSAIDNRPLQFPEFCLFPQRMIYLSAT
                     PGDYELEQTEGQVAELVVRPTGLLEPIIEIRPVLGQVDDILHEIRLRAGRNERVLITT
                     LTKRMAEDLTEYYDDLDVRVRYMHSDVDVVERTKILHDLRAGEFDVLVGINLLREGLD
                     LPEVSLVGIFDAD"
     gene            complement(1576..2376)
                     /gene="truA"
                     /locus_tag="EYO68_11285"
     CDS             complement(1576..2376)
                     /gene="truA"
                     /locus_tag="EYO68_11285"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIC08426.1"
                     /translation="MPSAQNWLLLLTYEGTCYHGWQVQHNSPTIEDSLEQAVRSLTGE
                     TVKVHGAGRTDSGVHALNFTANFTAIAENFKTAEKWRVALNAVLPPDIVVKYAQTVAA
                     DFHARHSAVGKRYRYLISNLPYKPPFSLNQSWWVSQDLDIDLMREAADLLVGKHDFSA
                     FRAASCSSPSSVKDLRVIEIFEVRSRHSTLQIEMEANSFLQHMVRIIAGTLVEVGRGY
                     KSVKDVAKALEGGLRKQAGITAPPQGLYSLSVIYPEGMIEWPLEVLDK"
     gene            complement(2386..2712)
                     /locus_tag="EYO68_11290"
     CDS             complement(2386..2712)
                     /locus_tag="EYO68_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HIC08427.1"
                     /translation="MASDQVIHLEDSNFEEETKSGYALIDFWAAWCGPCVSLAPLIDE
                     IAGDMEGKLKVCKLDVDKNMESAARFGVRGIPYIVLLKDGKEIDSVTGNDPSRVRALA
                     EQANAS"
     gene            3050..3379
                     /locus_tag="EYO68_11295"
     CDS             3050..3379
                     /locus_tag="EYO68_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIC08428.1"
                     /translation="MIEFSQQKVRQYLVHSFLYYQLGESIISDMQYDQICVEVETYLR
                     TNSNSNPLPYHDIITKSLAEDASGFSIRKYPEEIVSTAMHLLYQHNYRKSMTFDAFLS
                     RFGYSLL"
BASE COUNT         1004 a          810 c          747 g          995 t
ORIGIN      
        1 tgtctgcatc aaaaattcca accagcgaca cttccggtaa atccaaacct tcccgcagca
       61 ggttaattcc aaccaaaaca tcaaattctc cggcccgcag atcgtgcaaa atcttagtcc
      121 tctccacaac atccacatct gaatgcatgt agcgtactcg tacatcaaga tcgtcgtaat
      181 attcagtcag gtcttcggcc atccgttttg taagtgtggt aatgagtacc cgttcattac
      241 gtcctgcccg taaacggatt tcatgcagta tatcatcgac ttgtcccagc accggacgta
      301 tttcgattat aggttcaagc aaaccggtgg gacgcacaac gagttctgca acttgacctt
      361 cggtttgttc caactcataa tctcccggag ttgctgaaag ataaatcatt ctctgtggaa
      421 acagacaaaa ttcaggaaac tggagaggac ggttatcgat tgcagacggc aggcggaacc
      481 cgtgttccac cagagtcgat tttcttgaaa agtcaccttt gtacatggct cgaagctgcg
      541 ggagcgtcat gtgactttca tccacaaaaa tcagcgaatc atccggaaaa taattcagta
      601 atgttgccgg cggagttccg gctgccctcc ggtcaataat gcgactgtaa ttttcgattc
      661 cggaacatga gccagtctgc tcaatcattt caagatcgta ctcagtgcgc tgcaaaagac
      721 gctgtgcttc cagaattttg ttttgggcat tcaagtcttc gagacgttcg cgcagttctt
      781 cccggatcaa gctcaatgtt tccggaatat gttctgccgg agcaacataa tgcgaggcag
      841 gataaatgac aaaatgctga tatgacatct gctttttgcc ggtcagggga tcgaagcgaa
      901 ccaggctttc gatttcgtcg ccaaacaatt cgacccgaat tgcagattct gcttccgcca
      961 gataaatatc gatcacatca cctcgtaccc ggaaagttcc cctgtgaaaa tcgatatcgt
     1021 tgcgggtgta gagtagttca accagtcttt ccagaataac ctgccgcgaa aatcgttccc
     1081 caaccttaaa aataagcagc attttttcgt atttttcagg cgagccaatt ccataaatac
     1141 aggaaacact ggccacaatc agcacatccc gacgttccaa aagcgaacgc gttgccgaca
     1201 aacgtaaccg gtcgaggtcg tcattcagtg cagtatcttt ttcgatatag agatcacggc
     1261 tgggaacgta agcttccggc tggtagtagt cataatagct gacaaaatac tcgactgcat
     1321 tttcaggaaa gaaggtttgg aattcgttgt atagttgggc ggcaagcgtt ttattatgcg
     1381 caataaccag ggttgggcgc tgcagtgtct gaacaacatt ggccatcgcg aatgtttttc
     1441 cggaaccggt aatacccagc aaaacctggt gcttatgttt accttctaca ccatttacca
     1501 aagcctcaat ggcctgaggc tgatctccgg atggtttgaa ttctgattga agattaaatt
     1561 ccataaagat tactgctatt tatcgagcac ttccaatggc cactcaatca tgccttcagg
     1621 ataaatcacg ctcaatgaat agagcccttg aggcggtgcg gtaattccgg cctgctttcg
     1681 caaaccgccc tccaatgctt tggcaacatc tttgacgctt ttataccccc gtcctacttc
     1741 aaccagagtt ccggcaatta tgcgtaccat atgctgcaaa aacgaattgg cttccatttc
     1801 aatttgtaga gtagagtgac gggaacgaac ttcgaaaatc tctattaccc tgagatcttt
     1861 aaccgaactt ggtgacgaac aacttgctgc gcgaaatgcg gaaaaatcat gtttgccgac
     1921 taataaatct gcggcctcgc gcatcagatc aatatccaaa tcctgactta cccaccagct
     1981 ctgattcagg gaaaatggtg gcttgtaggg tagattggaa atcaggtagc ggtagcgttt
     2041 accgactgca ctgtgtctgg cgtgaaaatc tgctgcaaca gtttgggcgt atttgacgac
     2101 aatatcaggc ggtaaaactg cattcagcgc aacacgccac ttttctgccg ttttgaagtt
     2161 ttctgctatt gcagtaaaat ttgctgtgaa gttgagggca tgaactccgg agtcggtccg
     2221 ccctgcacca tgaactttaa ctgtttctcc ggtcaagctc ctgacagcct gttcaagtga
     2281 atcttcaatg gtgggtgagt tgtgctgaac ctgccatccg tggtagcagg ttccttcgta
     2341 ggtcagcaga agcaaccagt tttgtgcaga aggcacggga ggtttttaag aagcgtttgc
     2401 ctgttctgcc agtgcacgaa cgcgggatgg atcgtttcca gtaacactgt ctatttcttt
     2461 tccatctttg agcaggacga tgtaagggat tccgcgtact ccaaaacgtg ctgctgactc
     2521 catattttta tcgacatcaa gcttgcatac tttgagtttg ccttccatat ctccggcaat
     2581 ttcatcgatc aatggtgcta atgacacaca cggaccacac catgctgccc aaaaatcaat
     2641 gagcgcatag cccgatttag tttcttcttc aaaattggaa tcctcaaggt ggattacctg
     2701 gtcacttgcc atatattgct cctgctaggg taatgatttg agtctgtaat ttgcatgtat
     2761 taacagagtt ttttctgtaa aatacattta ctggagccta taattttgcc aaataaacat
     2821 gagaaatgca agttctttcc tgagatcata gagattctgc cccaaaaaca ctttaataat
     2881 tattgttgag attaaagatt aaaatggttt tgagttagaa attacccgaa aaatcttgct
     2941 aaataataaa aatcttctaa tttgaaacaa gagaaaatga tttgcaacta ccccatgcta
     3001 tcaagtttaa aatgcagact gcgctctttt catttacaat caatattaca tgattgagtt
     3061 tagtcaacaa aaagtccgtc agtatctggt tcattctttc ctttactatc aattgggtga
     3121 gtcaattatt tctgacatgc agtatgatca aatttgtgtt gaagtggaaa cctatttgcg
     3181 gacaaattct aattcaaatc ctcttccata tcatgatata ataactaaaa gtcttgcgga
     3241 ggatgcatcc ggcttttcca ttcgtaaata ccctgaagaa attgtcagca ccgcaatgca
     3301 cttgctctat cagcacaatt acagaaaatc catgaccttc gatgcgtttt tatcccgttt
     3361 tggatacagt ttactttgag ataacgaatg cctagaaaaa aagtcgtaaa tacaggtcca
     3421 aagaaagaaa agttcattcc cctggaagac gaaataattt cagaggaaga agttgttgtt
     3481 ccggaggaag aaaaaattcc agaggaagaa gagtctcttc agttactggg acaacaaatt
     3541 cgtgaagcac gaatta
//
